LOCUS       DASURG010000001         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_306487, whole genome shotgun sequence.
ACCESSION   DASURG010000001 DASURG010000000
VERSION     DASURG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306487"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFY80_00005"
     CDS             <1..223
                     /locus_tag="VFY80_00005"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX6006310.1"
                     /translation="VQRLLAYPDHYDYTASDARFIAQRAAGHLLLTTAKDAVKLRELL
                     AETPLHVAEQEVVFESGAGELMSALDNVL"
     gene            241..1797
                     /gene="nadB"
                     /locus_tag="VFY80_00010"
     CDS             241..1797
                     /gene="nadB"
                     /locus_tag="VFY80_00010"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX6006311.1"
                     /translation="MLVIGSGIAGLSFALKTARDHNVVLITKKERVESSTNYAQGGIA
                     SVMSADDSLELHVRDTLLAGAGLCHPSAVAGLVREGPARVRELMNWGVEFTRSGGDLS
                     LGREGGHSRRRIVHAADLTGREIERALLAALEAHEHVQVFEDYVAVDLLVGSDERSAD
                     KRCTGALALAHRTNELVQFHATVTLLATGGFGQAYRHTTNPEIATGDGLAMAYRIGAA
                     VANLEFVQFHPTALYPAHGHAFLISEAVRGEGAILRKLDGSVLETGHVLGSLAPRDVV
                     ARAIDMTLKESGEPHVWLDLSAIPPAQVRERFPNIVAHCEAQGIKVPRDPLPVVPAAH
                     YACGGVLTNAHGQTSIAGLFAAGEVSCTGVHGANRLASNSLLEAVVYSHRAASQVAVE
                     LAQARPTVERVTAPVVGRVVDETWSDVRRRLRDVLWENAGIVRSDERLEAAADSIRGL
                     QAEVARRFSDDGVSLEGIEARNLVDTAALVVRCARMRKESRGLHYNIDYPFRDNELFL
                     RDTVVTRENL"
     gene            1799..>2869
                     /locus_tag="VFY80_00015"
     CDS             1799..>2869
                     /locus_tag="VFY80_00015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX6006312.1"
                     /translation="MEKETIANINPTESPGANEENPFEAMMERFDQAAELLSLDPGLY
                     KVLRNPERQIITSIPITMDNGEIEVFKGYRVHYNTSRGPAKGGIRYDMNVSLDEVTAL
                     AAWMTWKCAVVDVPFGGAKGGVICDPFRMSQGELERLTRRYTASLIELLGPEQDVPAP
                     DVNTNEKMMAWIMDTYSMHKRHTVTAVVTGKPISIGGSRGRKEATGRGVMLSVKEALR
                     EVGMPLLGTRVVVQGFGNVGSVAAKLMQDEGLTIVAISDKTTGIYNPKGLNIDHALQW
                     IQQNRFLEGYTEGEQISNDEILELDCDVLTPCALENVITRRNAGRIKARVIAEGANGP
                     TTAPADAILEDKGVFVVPDILAN"
BASE COUNT          545 a          789 c          995 g          540 t
ORIGIN      
        1 ggtgcagcgt ctgctggcct atcccgatca ctacgactat acagcgagcg atgcgcgctt
       61 cattgcacaa cgtgcggcgg gtcatctgct gctcacgacc gccaaggacg cggtcaagtt
      121 gcgtgagctg ctggccgaga cgccgttgca tgtggccgag caggaggtcg tgttcgagag
      181 tggtgcgggt gagttgatgt ctgcattgga taacgtgcta tgagggtcgt ggctgcggat
      241 gtgctggtga tcggcagtgg cattgccggg ttgagcttcg ccttgaagac ggcgcgggac
      301 cacaatgtcg tgttgattac aaaaaaagag cgggtcgagt cgagtaccaa ctacgcgcaa
      361 ggcggtatcg catcggtgat gtcggcggat gattcgctgg agctgcacgt gcgcgatacg
      421 ttgttggcgg gggccgggct ctgccaccct tcggctgtag ccggattggt gcgcgaaggt
      481 ccggcgcgcg tgcgggagct gatgaattgg ggcgtcgagt tcacgcgcag cgggggcgac
      541 ctgtcgctcg ggcgcgaggg cggccattca cgacgtcgca tcgtgcatgc ggccgatctg
      601 accggacgcg agatcgaacg ggcgctgttg gctgcgctcg aagcgcacga acatgtgcag
      661 gttttcgagg actacgtcgc ggtcgatctg ctggtcggtt ccgatgagcg atcggcggac
      721 aagcgttgca cgggtgccct ggcgctggcg catcgcacga atgagctcgt ccagtttcat
      781 gcgacggtca cgctgctggc cacgggtggt tttggccagg cttaccggca tacgaccaat
      841 ccggaaatcg ccacgggcga tggtttagcc atggcgtatc gcattggcgc ggccgtggcc
      901 aacctggagt tcgtgcaatt tcacccgacc gcattgtatc ccgcgcacgg gcatgctttt
      961 ctgatttccg aggcggtacg gggcgaaggc gcgattttgc gcaagctgga cggttcggtg
     1021 ctggaaacgg gtcacgtgct cggttccctg gctccgcgcg acgttgtcgc ccgggcgatc
     1081 gatatgacgc tcaaggaaag cggcgagccg cacgtctggc tcgacctgtc ggccatcccg
     1141 ccggcgcagg tacgggagcg ttttcccaac attgtcgcgc attgcgaagc gcagggcata
     1201 aaagtgccac gcgatccact gccggttgta ccagccgcgc attacgcgtg cggcggcgta
     1261 ctgaccaacg cgcatgggca aacctcgatt gcgggtttgt tcgcggcggg cgaggtatcg
     1321 tgtactggcg tgcatggggc caatcgccta gcatcaaact ctctgctgga agcggtggtt
     1381 tacagtcatc gggcggccag ccaggtcgcg gtggaactgg cgcaggcgcg tccgacggtg
     1441 gagcgggtga cggctcccgt ggtcggccgc gtggtggacg aaacctggag cgatgtgcgc
     1501 cggcgtctgc gcgacgtgct gtgggagaac gccggcatcg tgcgaagcga tgaacggctg
     1561 gaagctgcgg cggacagcat acgcggcttg caggcggaag tggcgcgccg tttctcggac
     1621 gacggcgttt cgctggaagg catagaagcg cgcaatctgg tggataccgc ggccctggtc
     1681 gtgcgttgcg cgcgcatgcg gaaggaaagc cgagggctgc actacaacat cgactatccg
     1741 ttccgtgata acgagctgtt tttacgggac actgtagtaa caagagagaa tctctaaggt
     1801 ggaaaaagaa acgatcgcca acatcaaccc gacggaatcg ccgggggcga atgaagaaaa
     1861 tcctttcgaa gcgatgatgg agcgcttcga tcaggccgcc gagctccttt ccctcgatcc
     1921 ggggctgtac aaagtcctgc gcaatcccga gcggcagatc atcacttcga ttcccataac
     1981 catggacaat ggtgagatcg aagtgttcaa gggctatcgc gtgcattaca acacgtcgcg
     2041 cggtccggcc aagggtggca tccgctacga catgaacgtt tcgctcgacg aggtgaccgc
     2101 gctggcggcg tggatgacgt ggaagtgtgc cgtcgtcgat gtgccgttcg gcggcgcgaa
     2161 gggcggcgtg atttgcgatc ccttccgcat gtcgcaaggt gagctcgagc gtctgacccg
     2221 gcgttacacg gcgtcgctca tcgaattgct gggtcctgaa caggacgtgc cggccccgga
     2281 cgtcaacacc aatgaaaaga tgatggcgtg gatcatggac acctattcca tgcacaagcg
     2341 ccacacggtc accgcggtgg tgacgggcaa gccgatttcc atcggtggct cgcgtggccg
     2401 gaaggaagca accggacgcg gggtcatgct gtccgtgaag gaagcgctgc gtgaagtggg
     2461 tatgccgctg ctcggaaccc gcgtggtggt gcagggattc ggcaacgtgg gttcggttgc
     2521 ggccaagctg atgcaggacg aaggcctgac catcgtcgcc atcagcgaca agacgacggg
     2581 catctacaat cccaagggct tgaacatcga tcacgccctg caatggattc agcagaatcg
     2641 ctttctcgag ggttacaccg agggcgagca gatcagcaat gacgaaatcc tcgagctgga
     2701 ttgcgatgta ctgacgccct gcgcgctgga gaacgtgatc acgcgtcgca acgcgggcag
     2761 aatcaaggca cgcgtgatcg cggaaggcgc gaatggtcca acgacggcgc cggcggacgc
     2821 gatcctggag gacaagggtg ttttcgtggt gcccgacatc ctggccaac
//
LOCUS       DASURG010000002         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_204559, whole genome shotgun sequence.
ACCESSION   DASURG010000002 DASURG010000000
VERSION     DASURG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204559"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /gene="carB"
                     /locus_tag="VFY80_00020"
     CDS             <1..2006
                     /gene="carB"
                     /locus_tag="VFY80_00020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX6006313.1"
                     /translation="SDGLDERTTDRDVIENELGDPGPERIFYVADAFRCGMSIAEVHE
                     HAKIDPWFLAQIEDLVRQEQSLAGGTLNALDAETLRALKRSGFSDRRLAKLISTDQHA
                     VRARRHALSVRPVYKRVDTCAAEFATRTAYMYSTYEDECEAQPSTRRKIIVLGGGPNR
                     IGQGIEFDYCCVHAAFALREDGFESIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEI
                     VHVEQPHGVIVQFGGQTPLKLARDLEANGVPIIGTSPDSIDVAEDRERFQKLLNKLKL
                     KQPPNRTARNPQRALALAEEIGYPVVVRPSYVLGGRAMEIVYQQSDLERYMREAVKVS
                     NDSPVLLDRFLNDAIEVDVDAVSDGSTVIIGGVMEHIEEAGVHSGDSACSLPPFSLSG
                     ELQDALRRQTMAMAHALNVVGLINVQFAIQNSTIYVLEVNPRASRTVPFVSKATGLPL
                     AKIAARCMAGRTLAEQGVEREIVPPYYAVKEAVFPFIKFPGADTILGPEMKSTGEVMG
                     VGKSFGEAFVKSQMAAGEKLPATGRVFISVRDADKPRTVEIARALAKLGFTLVATRGT
                     AAALAAAGLNVTPVNKVAEGRPHIVDMIKNGEIALIVNTVEEKRTAVRDSYSIRRAAL
                     QGRITLYTTLSGARAACNGMEQSAELRAYVVQALHAQLREAVH"
     gene            2031..2507
                     /gene="greA"
                     /locus_tag="VFY80_00025"
     CDS             2031..2507
                     /gene="greA"
                     /locus_tag="VFY80_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX6006314.1"
                     /translation="MGKIPLTIAGAEKLRAELQELRTVQRPAIINAISEARSHGDLSE
                     NAEYHAAKERQSFVEGRIAEIEAKLANAQIINPALLDADGRCVFGATVELEDIANREV
                     VTYQIVGEDEADIKQGKISISSPIARALIGKYSGDVAEVDAPGGTREYEILDVKYI"
     gene            complement(2547..2906)
                     /locus_tag="VFY80_00030"
     CDS             complement(2547..2906)
                     /locus_tag="VFY80_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEX6006315.1"
                     /translation="MADITPAARRALRARAHRLHPVVIIGEAGLTPAVLKEIDAALRS
                     HALIKIRVLGEDRALRRAWMTEICQKLDAAAVQEIGKLLVVHRPRPPQTAVQSVPRRH
                     GRKEKRPRRTKRSYQMQ"
     gene            2924..3547
                     /locus_tag="VFY80_00035"
     CDS             2924..3547
                     /locus_tag="VFY80_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6006316.1"
                     /translation="MARTPSSKRWLREHVTDPYVRQARNEGMRSRAAFKLKEIAERDR
                     LFGPGMTVIDLGAAPGGWSQVAARAVGASGQVYALDRLDFPPLPGVIRIQGDFEDEAT
                     VLALEKRLGGASADLVLSDMAPNISGVAALDQARTVALAERALEFALKHLKPQGKFLV
                     KVFHGAGYEAFLKTMRGRFHAVVVRKPDASRSRSREVYLLGRGVKSA"
     gene            3687..>4507
                     /locus_tag="VFY80_00040"
     CDS             3687..>4507
                     /locus_tag="VFY80_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX6006317.1"
                     /translation="MTVFNQFSTRQTTQKAMEYSQFIDEVKQGRIAKVTIEGRVLKGV
                     KTSGERFTTYSPSDPWLVSDLLKNGVIIEAKPEEEPSLLMNIFVSWFPMLLLIGVWIF
                     FMRQMQGGGRGGAFSFGKSRARMLDESNNTVTFADVAGCEEAKEEVAELVEFLRDPSK
                     FQKLGGRIPRGVLMVGNPGTGKTLLARAIAGEAKVPFFSISGSDFVEMFVGVGAARVR
                     DMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFEANEG"
BASE COUNT          849 a         1351 c         1520 g          787 t
ORIGIN      
        1 gcagcgacgg actggacgag cgcacgacgg accgggatgt gatcgaaaac gagctcggcg
       61 acccaggtcc cgagcgcatc ttctacgtcg ccgacgcgtt tcgctgcggc atgagcatcg
      121 ccgaagtcca cgaacacgca aaaatcgatc cgtggttcct ggcgcagatc gaggatctcg
      181 tgcgccagga gcagtcgctt gccggcggca cgctgaacgc gcttgacgcc gagacgctga
      241 gagccttgaa gcgcagcggt ttctcggacc gccgcctcgc gaagctcatc tccaccgacc
      301 agcacgcggt gcgtgcgcgc cggcacgcgc tcagcgtgcg accggtctac aagcgggtgg
      361 acacgtgcgc ggcggaattc gcgacccgca cggcctacat gtattcgacc tacgaggacg
      421 agtgcgaggc gcagccgagc acgcgcagga agatcatcgt gctgggcggc gggccaaacc
      481 gcatcgggca gggcatcgaa ttcgactact gttgcgtgca cgcggccttc gcactgcgcg
      541 aggacggttt cgagagcatc atggtgaact gcaacccgga gaccgtctcg actgactatg
      601 acacgtccga ccggctctat tttgagccgc ttacgctcga ggacgtgctc gagatcgtgc
      661 acgtcgagca gccgcacggc gtcatcgtgc agttcggggg gcagacgccg ctcaagctcg
      721 cccgcgatct cgaagcgaac ggcgtgccga tcatcggaac atcgccggac tccatcgatg
      781 tcgccgagga ccgcgagcgc ttccagaagc tgctcaacaa gctcaagctg aagcagccgc
      841 ccaatcgcac ggcgcgcaac ccgcaaaggg cgctcgcgct tgccgaggag atcggctacc
      901 cggtggtggt gcggccgtcc tacgtgctgg gcggccgcgc catggagatc gtctaccagc
      961 agagcgatct cgaacgctac atgcgcgaag cggtgaaggt ttcgaacgac agccccgtgc
     1021 tgctcgaccg cttcttgaac gatgcgatcg aagtggacgt cgatgccgtg tccgacggca
     1081 gcaccgtgat catcggtggc gtgatggagc acattgagga ggcgggggtc cattccggag
     1141 actccgcctg ctcgctgccg cccttcagcc tctccgggga actccaggac gcgctgcgcc
     1201 gtcagaccat ggcaatggcg cacgccctca acgtcgtggg cctcatcaac gtccagttcg
     1261 cgattcagaa cagcacgatt tacgtgctcg aagtgaatcc ccgggcttcc cgcaccgtgc
     1321 cgttcgtatc gaaggcgacc gggctcccgc tcgcaaaaat tgcggcacgc tgcatggcgg
     1381 gcaggacgct tgccgagcag ggcgtcgagc gtgagatcgt gccgccgtat tacgcggtga
     1441 aggaggcggt gtttccgttc atcaagtttc cgggggcgga tacgatactg ggacccgaga
     1501 tgaaatcgac cggcgaggtg atgggtgtcg ggaagagttt cggcgaggcc ttcgtgaaat
     1561 cgcagatggc ggcgggagag aagctgcccg caacaggcag ggtattcatc agcgtgcgcg
     1621 acgcggacaa gccgcggacg gtggaaatag cccgtgcgct ggcgaagctc ggttttacgc
     1681 tggtcgccac gcgcgggact gccgccgcgt tggcagccgc cgggctcaac gtgacgccgg
     1741 tcaacaaggt ggccgaaggc cggccgcaca tcgtcgacat gatcaagaac ggagagatcg
     1801 cgctcatcgt gaacaccgtg gaggaaaaac gcacggccgt gcgcgactcg tattcgatcc
     1861 gccgcgcggc gctgcagggc cggattacgc tctacacgac gctctcgggt gcgcgggccg
     1921 cgtgcaacgg catggagcag tccgccgagc ttcgcgcgta cgtcgtgcag gcgctccacg
     1981 cacaattgcg cgaagcggta cactgaaaat acgctccgag ggagcctggg atgggtaaaa
     2041 ttcctttgac cattgccggt gcggagaaat tgcgcgccga gctgcaggag ctgcgcaccg
     2101 tgcagcgccc ggccatcatc aacgcgattt cggaggcacg ctcgcacggt gacctctcgg
     2161 agaacgcgga atatcacgcc gctaaggagc ggcagagctt cgtcgaggga cgcatcgccg
     2221 aaatcgaggc gaagctcgcc aatgcgcaga tcatcaatcc cgcactgctg gacgcggacg
     2281 ggcgctgtgt cttcggtgcg accgtggagc tcgaggatat cgcgaatcgg gaggtggtca
     2341 catatcagat cgtcggcgaa gacgaagcgg atatcaagca gggcaagata tcgatcagct
     2401 cccccattgc gcgcgcgctg atcggcaagt attccggcga cgtcgctgaa gtcgatgcgc
     2461 ccggcgggac gcgtgaatac gaaatcctcg acgtcaagta catctgacgg gctttcgaac
     2521 gtgcgctgcg cgccgcattc cggacgctat tgcatctggt agctgcgctt ggtgcggcgc
     2581 gggcgctttt ccttcctgcc gtgtcggcgt ggcaccgact gcaccgcggt ctgcggcggt
     2641 cgtggccggt gcacgacgag cagttttccg atttcctgca ccgctgcggc gtcgagcttt
     2701 tggcaaattt ccgtcatcca ggcgcggcgc agcgcgcggt cttcgcccag gacacgaatc
     2761 ttgatcagcg catggctgcg cagagcggca tcgatttctt tcagcaccgc cggggtgagg
     2821 ccggcttcgc cgatgatgac aacggggtgg agtcggtggg cgcgcgccct gagcgcccgg
     2881 cgcgcggccg gcgtaatatc agccatggga cgagtgtagc gcaatggcgc ggaccccgag
     2941 cagcaaacgc tggctgcgcg agcacgtcac ggacccttac gtccgccagg cgcgcaacga
     3001 agggatgcgt tcgcgagccg ccttcaagtt gaaggaaatt gccgaacgtg accgattgtt
     3061 cggtcccgga atgacggtca tcgaccttgg cgcggcgccg ggcgggtggt cgcaggtagc
     3121 ggcgcgggcg gtgggcgcct ccggacaggt gtatgcgctc gaccggctcg atttccctcc
     3181 gcttccgggt gtgatccgga tccagggcga cttcgaggac gaggcgaccg tactggccct
     3241 tgaaaagcgc ttgggcggcg cgagcgcgga ccttgtgctt tccgatatgg cgcccaatat
     3301 cagtggggta gcggcgcttg accaggcgcg caccgtggcg ctcgccgagc gcgcgctcga
     3361 gttcgcgttg aagcatttga aaccgcaagg aaagttcctg gtcaaagtgt tccatggcgc
     3421 gggctacgag gcctttctca aaacgatgcg gggacgcttt cacgccgtcg tcgtgcgcaa
     3481 gcccgacgct tcgcgcagcc gctcgaggga ggtgtacctg ttaggcagag gcgtgaagag
     3541 cgcttgaggg ctttgctgca ctgcaagcat cattacggtt acaatcctcg gagatgcact
     3601 tggccgtgag ccgtgcatgt taaggagcat tcttgaataa tctgatcaaa aacgtggcga
     3661 tctggctcgt catcgcgctc gtgctgatga cagtgttcaa tcagttcagc acgcgccaga
     3721 cgacccagaa ggcgatggag tattcgcagt tcatcgacga ggtgaagcag gggcgcatcg
     3781 ccaaggtcac gatcgaaggc cgcgtgttga agggcgtcaa gacgagcggc gagcgcttca
     3841 cgacctattc gccgtccgac ccctggctcg tgagtgatct gctcaagaat ggggtgatca
     3901 tcgaggcgaa gccggaagag gagccttcgc tcctcatgaa catcttcgta tcgtggttcc
     3961 cgatgctgct cttgatcggc gtttggattt tcttcatgcg ccagatgcag ggcggcggcc
     4021 gcggcggcgc attttcgttc ggcaagagcc gcgcgcgcat gctcgacgaa tcgaacaaca
     4081 ccgtgacgtt tgccgatgtc gccggatgcg aagaggccaa ggaagaagtg gccgagctgg
     4141 tcgaattcct gcgcgatccc tcgaaattcc agaaattggg cggtcgcatt ccgcgcggcg
     4201 tgctgatggt cggcaatcca ggaaccggca agacgctgct cgcgcgcgcc atcgccggcg
     4261 aagcaaaggt gccgtttttc tcgatttccg gctcggattt cgtcgagatg ttcgtcggcg
     4321 taggtgctgc ccgcgtccgc gacatgttcg agcaggcaaa gaagcatgcg ccgtgcatcg
     4381 tcttcatcga cgagatcgat gcggtgggtc gccagcgcgg cgcagggctg ggcggcggca
     4441 atgacgagcg cgagcagacc ttgaaccagc tcctggtcga gatggacggg ttcgaggcca
     4501 acgaggg
//
LOCUS       DASURG010000003         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1227256, whole genome shotgun sequence.
ACCESSION   DASURG010000003 DASURG010000000
VERSION     DASURG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227256"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..196
                     /locus_tag="VFY80_00045"
     tRNA            124..196
                     /locus_tag="VFY80_00045"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:157..159,aa:Asn,seq:gtt)
     gene            231..1046
                     /locus_tag="VFY80_00050"
     CDS             231..1046
                     /locus_tag="VFY80_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006318.1"
                     /translation="MNCFRAGVRTLRGFAGVAAMFVSAVPAWAQTDYYNTDAGRPLQV
                     EDAYPVERRGFEVQAAPLRLARSRGGVYEWGIEPEIAFGVAARTQVEVSAPFAFIDAG
                     DAQTAGLSGLELSALRNLNAETSIPAFAIGAHVLLPVGNLGPDETYASVKGIMTRTFT
                     WARFHVNAEYTFGSDRPGAAGAAELSRWMTGIAVDRAFPLHSLLIGAELFAREPIVNE
                     VDVEWDTAVGLRYQLTTRFNIDGGIGRHLTGDDRAWYLTFGSAYAFGLPWHRR"
     gene            1043..>2149
                     /locus_tag="VFY80_00055"
     CDS             1043..>2149
                     /locus_tag="VFY80_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006319.1"
                     /translation="MNAPRSRRARRWYLRGVGIALVLAGASLASSCAQWSTTYDQFYL
                     QASHNWQFRHRYQSADRLFNAFDFGHAILYETLWTKPNADAAELETKWYGRLTESILV
                     RPPRLPLEEAAIEIAYAKLAPEAKAMFDWAHILHRQIYDVLADERLSQAAKDAEITRL
                     VAYYRTRDDLAFSSRPKSMALMQEQPYSLAFRQRYPKFNGLIWAYHWLQVGLYEPLLT
                     SPSSDARQSRVRATVARFWQMLEDPPRTMPHVMPMTSVVAPTFTARYPDAAVIFDNLH
                     SMHDVVSDILANPEVPRERKRAELLLAARRFRDDSSYVMSLDAWRAMAHHMGVENMGG
                     PAMDFLPVLPTPTVTYGAVMTHDDRTGEMTGFKYG"
BASE COUNT          351 a          682 c          726 g          390 t
ORIGIN      
        1 gagacgggcg cggcgcgagc gcggtcaccc gagcgagcgc cgaattcgcg catgtcaccg
       61 aaaggcgaat gaaggccctc tcatgggcag acggcgggtg ggtcactagc tttcagtcgc
      121 cggtggggcg tagctgagct ggcaaagcgc cggactgtta atccggagac cggtggttcg
      181 aatccacccg ccccagtcct ggtactcata acgcgaagag gttgtcgttg atgaattgct
      241 ttcgcgccgg ggtgcgcacc ctccgcggct tcgctggggt ggcggcgatg ttcgtgtcgg
      301 cggttcccgc gtgggcgcag accgactact acaacaccga tgccgggcgc ccactccagg
      361 tcgaggatgc gtatccggta gagcggcgtg ggttcgaggt ccaggcagcg ccgcttcggc
      421 tggcgcggag ccgcggcggg gtctacgagt ggggaatcga gccggagatc gcgttcggag
      481 tggccgcccg cacgcaggtc gaagtcagcg cgccgttcgc cttcatcgat gccggcgacg
      541 ctcagacggc cggcctttcc ggactcgagc tctccgcgct gcggaacctg aacgcggaga
      601 cgagcatacc ggcattcgcg atcggcgcgc atgtcctgct gcccgtgggt aacctcggcc
      661 ccgacgagac ctatgcgtcg gtgaagggaa tcatgacgcg cacgttcacc tgggcgcgtt
      721 ttcatgtgaa tgcggagtac acgttcggct ccgatcggcc cggggcggct ggcgcagcgg
      781 agctgtcgcg atggatgacg ggtatcgcgg tggatcgtgc gttcccgttg cattcgctgc
      841 tcatcggggc cgagctgttc gcccgtgagc cgatcgtgaa cgaggtggac gtggagtggg
      901 acacggcggt cgggctccgg taccagctca cgacgcgctt caacatcgat ggtggcatcg
      961 gccggcacct aacgggggac gaccgcgcct ggtacctcac gttcggctcg gcgtacgcat
     1021 tcgggcttcc atggcaccgg cgatgaacgc accccgatcg cgccgcgcac gccgctggta
     1081 ccttcgcggt gtcggcatcg cactggtact tgcgggcgca tcgctcgcgt cgtcgtgcgc
     1141 gcaatggtca acgacgtacg accagttcta tctgcaggcg agccacaact ggcaattcag
     1201 gcatcggtat cagtcggcgg accggctctt caacgcgttc gacttcggcc atgcgattct
     1261 ttacgaaacg ctctggacga agcccaacgc cgacgccgcg gagctcgaga ccaagtggta
     1321 cggacggctg actgagtcga tcctcgtgcg tccgccgcgc cttccactcg aggaagccgc
     1381 aatcgagatc gcctacgcga agctggcgcc ggaagcgaag gcgatgttcg attgggcgca
     1441 catcctccat cgccagatct acgatgtgct ggcggacgag cggctgtcgc aggcggcgaa
     1501 ggatgcggag atcacccgcc tcgtcgccta ttaccggacg cgcgacgatc ttgccttcag
     1561 ctcccgcccg aagtcgatgg cgctgatgca ggagcagccc tattcgctcg cctttcgtca
     1621 gcgctatccg aaattcaacg ggctgatctg ggcatatcac tggcttcagg ttgggctcta
     1681 tgagccgctc ctcacgagtc cgtcctccga cgcgaggcag tcgcgggtgc gcgcgacggt
     1741 cgcccggttc tggcagatgc tggaggaccc gccgcgcacc atgcctcacg tgatgccgat
     1801 gacctcggtg gtcgctccga cctttacggc gcgctacccc gatgccgccg tgatcttcga
     1861 caacctgcac tccatgcacg atgtcgtatc ggacattctc gcgaaccccg aggtgcctcg
     1921 cgagcggaag cgcgcggagc tcttgctggc ggcgcgtcgc tttcgcgacg actcgtcgta
     1981 cgtgatgtcg ctcgacgcct ggcgggcaat ggcgcatcac atgggcgtcg agaacatggg
     2041 tgggccggcg atggactttc tcccggtgct cccgactcca acggtcacct atggggcggt
     2101 gatgacgcac gatgatcgga ccggcgaaat gaccggtttc aagtatggc
//
LOCUS       DASURG010000004         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_2146, whole genome shotgun sequence.
ACCESSION   DASURG010000004 DASURG010000000
VERSION     DASURG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..582
                     /locus_tag="VFY80_00060"
     CDS             25..582
                     /locus_tag="VFY80_00060"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6006320.1"
                     /translation="MEFPQRLKTPRLLLARLEGSDFEDYLRRCGGALFSEGHPSLHDE
                     PAIRFRAHVDHWQRHGYGWWAVRDPQTRAYRGCGGLESAVLDGKRTTLVTCDGWDRDY
                     AAELVRIAVAQGFVGLDVQEIVAAIAPPPPAAARVLERAGFIRERAGAASRDAPAIYR
                     LTARSWCASPRTRDSGRAPAAIQTV"
     gene            586..1200
                     /gene="pth"
                     /locus_tag="VFY80_00065"
     CDS             586..1200
                     /gene="pth"
                     /locus_tag="VFY80_00065"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6006321.1"
                     /translation="MKLLVGLGNPGRKYEHTRHNAGFWLIERLAAKSGIALRNEAKFQ
                     ALVGRHEVTGTWLLMPQAYMNASGRPVQMLASFFKIAPQDILVAHDELDFPPGTVRLK
                     LGGGVAGHNGLKDISARLGTHDYWRLRIGIGHPGARDLVTHYLLNSHPTAEERASIDG
                     AIDRALEVMPLILSGDMQGAMHSLHTEDKQPKADAHETDRTRQQ"
     gene            1197..2291
                     /gene="ychF"
                     /locus_tag="VFY80_00070"
     CDS             1197..2291
                     /gene="ychF"
                     /locus_tag="VFY80_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX6006322.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNALTKAGISAENYPFCTIAPNVGVV
                     EVPDPRLAQLAELAKPQKVIPAVVEFVDIAGLVAGASKGEGLGNQFLANIREADAMAH
                     VVRCFEDPNVTHVAGRIDPVSDIETIDTELALADLASVERQLAKYGKVARAGGDKEAQ
                     RLVAVLEKCQAALHEGKPVRALALYEEEKTVLRTLFLLTAKPVMYVANVREDGFEGNA
                     HLDATREYAAREGAPVVAVCAAIEAEIADMADQDKRMFLADMGMSEPGLDRVIHTSYA
                     LLGLQTFFTAGPKEVRAWTIHRGDSASRAAGVIHTDFEKGFIRAEVIAFDDYIACKGE
                     QGAKEAGRMRLEGKEYVVRDGDVIYFRFNV"
BASE COUNT          422 a          752 c          737 g          382 t
ORIGIN      
        1 cttttttgac gttacggatt gcccatggaa ttcccccaac gactcaagac gccgcgcctt
       61 ctccttgcac gcctcgaggg gagcgatttc gaggattatc tccgccggtg cggtggcgcg
      121 ctcttctccg agggccaccc ctcgctccac gatgagcccg ccatacgttt tcgcgcccac
      181 gtcgaccact ggcagcgcca tggatacggc tggtgggcag tgcgcgaccc gcagacgcgg
      241 gcctaccgcg gctgcggcgg cctcgagtcg gccgtgctgg atggaaagcg gaccaccctc
      301 gtgacctgcg acggctggga tcgcgattat gcggccgagc tggtacggat tgcggtcgcg
      361 cagggcttcg tggggctcga cgtgcaggaa atcgtcgcag cgattgcgcc cccaccgcca
      421 gctgcggcac gcgttctcga gcgggcggga tttatccgcg agcgtgccgg cgctgccagc
      481 cgcgatgccc ctgccatcta tcggctgacc gcgcgatcat ggtgcgcgtc gccgcggacg
      541 cgggatagcg gccgcgcccc ggctgccatc cagacggtct agccggtgaa attgctcgtc
      601 ggtctcggca atcccggtcg caagtacgaa cacacgcgac acaatgccgg cttctggctg
      661 atcgagcggc ttgcggcaaa aagcggaatc gcgctccgca acgaagcgaa gttccaggcg
      721 ctggtcgggc gccatgaggt gaccggcacg tggctgctca tgccccaggc ctacatgaac
      781 gcgagcggcc gaccggtgca gatgctggca agcttcttca agatcgcgcc ccaggatatt
      841 ctcgtcgccc acgacgagct cgactttccg ccggggaccg ttcggctcaa gctcggcggc
      901 ggcgtggccg gtcacaacgg cctcaaagac atctcggcgc ggctcggcac gcatgactac
      961 tggcgcttga ggatcgggat cggccacccc ggcgcacgcg atcttgtcac gcattacctg
     1021 ctgaacagcc atcccactgc ggaagagcgc gcgagcatcg atggtgcgat cgaccgcgcg
     1081 ctggaggtca tgccgctcat tctatccggc gacatgcagg gcgcgatgca tagtctccac
     1141 accgaggaca agcagcccaa agcagacgcg cacgagaccg atcggacgcg gcagcaatga
     1201 gcctccagtg cggcatcgtc ggactgccca atgtcggcaa gtccacgctc ttcaatgcgc
     1261 ttaccaaagc gggcatctcc gcagagaact acccgttctg caccatcgcg cccaatgtcg
     1321 gcgttgtcga agtgccggac ccgcgcctcg cccagctcgc cgagcttgcg aaaccgcaga
     1381 aggtcattcc cgcggtcgtg gagttcgtcg atatcgccgg gctcgtggcg ggcgcatcca
     1441 agggagaggg gcttgggaac cagtttctcg ccaacatccg cgaagccgat gcgatggcac
     1501 atgtcgtgcg ctgcttcgaa gatcccaatg tgacgcacgt tgcgggcagg atcgatcccg
     1561 tttcggacat cgagacgatc gacaccgagc tcgcgcttgc cgatctcgcg agcgtcgaac
     1621 gccagctcgc gaagtatggc aaggtcgcgc gcgccggcgg cgacaaggaa gcccagcgcc
     1681 tcgtcgccgt gctcgagaaa tgccaggctg cactccacga gggcaaaccg gtacgcgcgc
     1741 tcgctctcta cgaggaggag aagacggtgc tccgcacact cttcctcctt accgccaaac
     1801 ccgtgatgta cgtcgcgaac gtgcgagaag acggcttcga gggcaatgcc catctcgatg
     1861 cgacccgcga atacgctgca cgcgaaggcg caccggtcgt cgctgtttgc gcggcgatcg
     1921 aagcggagat cgcggatatg gcggatcaag acaagcgcat gtttctcgcc gacatgggca
     1981 tgagcgagcc cggcctcgat cgcgtcatcc acaccagcta tgcgctgctc ggcctgcaga
     2041 cttttttcac cgccggcccg aaggaagtgc gggcgtggac gatccatcgc ggggatagcg
     2101 catcgagggc tgccggcgtg atccacaccg atttcgagaa gggcttcatc cgcgccgaag
     2161 tcatcgcttt cgacgactac atcgcctgca agggcgagca gggcgcgaag gaagccggga
     2221 ggatgcggct ggaaggcaag gaatacgtgg tccgggacgg cgacgtcatc tatttccgct
     2281 tcaacgtgtg acc
//
LOCUS       DASURG010000005         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_2285, whole genome shotgun sequence.
ACCESSION   DASURG010000005 DASURG010000000
VERSION     DASURG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..386)
                     /locus_tag="VFY80_00075"
     CDS             complement(81..386)
                     /locus_tag="VFY80_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006323.1"
                     /translation="MVPKERRKRRLGLKVIHKHRVGDKVLRRVSELVIIEGQPKALLG
                     WINFGGVRTPIYLCDLDLAKLHRSATSTSTFYYEEITVDPRFEGLAPGQQDDPLQKG"
     gene            524..599
                     /locus_tag="VFY80_00080"
     tRNA            524..599
                     /locus_tag="VFY80_00080"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Ala,seq:cgc)
     gene            837..1724
                     /locus_tag="VFY80_00085"
     CDS             837..1724
                     /locus_tag="VFY80_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006324.1"
                     /translation="MSKTRFGFWPRPWHIAAAGLSVEPVADCADVVEFINKHERALDG
                     WMHPPLGRRFSSALGLSVPWMPATAYNLPSTHELNLDTDDEALADFSIALIGLLDGMR
                     LVREGWNHFYKTPIEPAKLVDFVCTQDEMSRAVDMATRRWRGLDRDCQKWMFGAIHWY
                     CFTAAYEHQFERFGGLYIVLDTLYRVHVRLGGSAADTRAEQAAVLADAHRIPLPDWAQ
                     VHTVDNKEVSRLSTLRNEFFHEGRYGGEPVGFDAPEENITLDFEAFVTRVILGILSID
                     CAYVRTPVTTQQKQGLDVS"
     gene            complement(1759..1884)
                     /locus_tag="VFY80_00090"
     CDS             complement(1759..1884)
                     /locus_tag="VFY80_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006325.1"
                     /translation="MSQCQNLIGADRDKCLQDERAATMGSGGGAGTSAPPAGSIR"
     gene            1940..2077
                     /locus_tag="VFY80_00095"
     CDS             1940..2077
                     /locus_tag="VFY80_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006326.1"
                     /translation="MAEGACDGVTDGDCDGVTDGDCDGVTAGVSDGVVLGGVKPDWGW
                     A"
     gene            2294..>2890
                     /locus_tag="VFY80_00100"
     CDS             2294..>2890
                     /locus_tag="VFY80_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6006327.1"
                     /translation="MTPLRLVAALLQRLVDKIEPHEGRYRMLFERSPLPMWVVDTQTL
                     RFLAVNDAALRLYEYSRDEFLAMTALDIRGAAEDALFREFVSREGGSVRHGVFRHQKK
                     TGDPVDIDGVGHLVAWRGRPARLVQINDITERLRTQQTLERLNRELELSHERLRALSR
                     RLFEVQEEERRRLARDLHDDVGQALTALKIQLESVARAG"
BASE COUNT          508 a          880 c          925 g          577 t
ORIGIN      
        1 catcaacgcc ccggcgctgg ccgcgccgga aaagggcaaa cccacgcgcg tcgatttcgg
       61 attgaggtga gggagcggcc tcagcccttt tgcagcgggt catcctgctg cccgggcgcg
      121 agcccctcga accgcggatc caccgtgatt tcttcgtagt agaaagtgct cgtgctggtc
      181 gcggagcggt gcagcttcgc gaggtcgagg tcgcagaggt aaatcggggt gcgcacgccg
      241 ccgaaattga tccagccgag aagtgccttg ggttgccctt cgatgattac gagttccgag
      301 accctgcgca ggaccttgtc gccgacccgg tgcttgtgga tcaccttcaa ccccagccgc
      361 cttttcctgc gctccttggg caccattccg ggctccgaaa ccaatcgagc aggagtcgtg
      421 ccgctgcgct caggtcattg acctcatgta gtccgggtcg tgacaccggg agagttcggt
      481 ctgtaggatg caaagcatgt tgcggtagaa tccgccgctc tgcggggtgg tagctcagct
      541 gggagagcgt cgcgttcgca atgcgaaggt cgggagttcg atcctcctcc actccaccaa
      601 cctctcaccg tgggaccgca accggacgga ccagcgcacc gaagcgggcc tatgttttta
      661 ttatattgac gctttctcgc cgggcttcaa gcggccgcgt cacgctccgg gggtgctgct
      721 aattgatgca ttcacccttc aagagtcgct cacgcagaat tttccggtat aagcgcccac
      781 agggagggcg agtcagtgga cgatagcact gccagcctga ggccttgtcg gcgggtatga
      841 gcaaaacccg tttcggcttt tggccgcgcc cttggcacat tgcagcagca gggctcagtg
      901 tcgaaccggt tgcagactgc gctgatgtcg tcgagtttat taacaagcac gaaagagcgc
      961 ttgacggatg gatgcatccg ccgcttggac gacgcttttc ctcggctctc ggtctatctg
     1021 ttccatggat gcccgcaacg gcatacaacc tgccgtccac tcacgagctg aacctggaca
     1081 ctgacgacga ggctctcgcc gacttctcca tagccttgat tggccttttg gacggcatgc
     1141 gccttgtccg agaaggttgg aatcacttct acaagacgcc aatcgagccc gcgaagctgg
     1201 ttgacttcgt ttgcacgcag gacgagatgt cgcgggcagt ggatatggcc acgcgccgtt
     1261 ggcgtggtct ggatagggat tgtcaaaaat ggatgtttgg cgcgatccat tggtattgct
     1321 tcaccgcagc ctatgagcac caattcgagc gattcggtgg cctgtacatc gtgttggata
     1381 cgctatatcg cgttcatgtg cgactaggcg gtagtgcggc ggacactcgt gcggagcaag
     1441 cagcggtcct cgcggacgcg cacagaatcc ctcttcccga ttgggcgcaa gtccacacgg
     1501 tcgacaacaa agaggtgtcg cgtctttcca cgctgcggaa cgagttcttc catgaaggcc
     1561 ggtacggcgg cgagcctgtt ggcttcgatg cccccgaaga aaacatcacc cttgactttg
     1621 aggccttcgt aacgcgggtc attctaggga tcctgtctat tgattgtgcc tacgtgcgga
     1681 ccccggtcac gacgcaacag aagcagggcc tcgatgtttc ataggttgct tggcccgcaa
     1741 cgagcaaggc tctgagcatt accgtatcga gcccgcgggc ggtgcgctgg tgcccgcgcc
     1801 gcctccgctg cccatggttg cggcccgctc gtcctgcagg cacttgtctc tgtcggcgcc
     1861 gatcaggttc tggcattggc tcatgccgcc gacagctcgc ggtgccgtcg gcgcggtcgc
     1921 tccggaagat ggagatggag tggcagaagg cgcctgcgac ggcgtgaccg atggcgattg
     1981 cgacggcgtc accgatggcg actgcgatgg cgtgacggcg ggcgtgtcgg acggcgttgt
     2041 gctcggcggc gtcaaaccgg attggggctg ggcataggct gcgctcgcaa gcgcgagcaa
     2101 cattgcagag gcgactgact tgagggtcat tgcattctcc atcaaaggaa tcggtgtgcc
     2161 gccagccgca agctgcgtgc caccacgtcg gcctacggcg ctcgcgatag gccattttgc
     2221 cggagtcacc taaggcgcgg ccgcgagcac cgtgccggcg caaaccgcgg gccagcgtta
     2281 ccattcccgc tcgatgaccc cgcttcgcct cgtcgccgcc ctgctacagc gccttgtcga
     2341 caagattgaa ccgcacgagg gacgctaccg gatgctgttc gaacgcagcc ccttgcccat
     2401 gtgggtcgtg gatacgcaga cgctgcgttt tcttgccgtc aatgacgctg ctctgcgtct
     2461 gtacgagtac tcgcgcgacg agttcctcgc gatgactgcg ctcgacatcc gcggcgcggc
     2521 ggaagatgct ttgtttcgcg agtttgtgag ccgggaaggc ggctcggtgc gccacggcgt
     2581 cttccggcac cagaagaaga cgggcgatcc ggtcgacatc gacggcgtcg gccacctggt
     2641 cgcatggcgc ggccgaccgg cgcggctggt gcagatcaac gacatcaccg agcgcctgcg
     2701 cacgcagcag acgctcgagc ggctcaatcg cgagctcgag ctttcgcacg agcggctgcg
     2761 cgcgctgtcg cgccgcctgt tcgaagtgca ggaagaggag cgccggcgcc tcgcgcgcga
     2821 cttgcatgac gacgttggtc aagcccttac tgcgctaaag atccaattgg agtcggtggc
     2881 gcgggccggc
//
LOCUS       DASURG010000006         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1125464, whole genome shotgun sequence.
ACCESSION   DASURG010000006 DASURG010000000
VERSION     DASURG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125464"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1772
                     /locus_tag="VFY80_00105"
     CDS             123..1772
                     /locus_tag="VFY80_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6006328.1"
                     /translation="MNAHFSPTDGAYTAHVDTFARDRLPPREQWPEFRFDLPELRYPP
                     RLNCASVLLDEAILEGHGARVALYSQGGNWSYFELLERANRIANVLVRDLGVVPGNRV
                     LLRGANTPMLVASWLAVMKVGAIAVTTMPMLRARELIVMAERAQVDHALCEARLAGEL
                     REAAQATRRLSRVATYGDGHLEGLMAQRTDEFRNVATAAEDVSLLAFTSGTTGNPKAT
                     MHFHRDVLVMRDVVGRYLLETSPDDIYTGSPPLGFTFGLGALLVFPLGGRGAVALVEQ
                     PTPDNLLDTIARFRATALFTAPTAYRALLPLVPGRDLASLRRCVSAGEALPKATSDAW
                     HAATGLRIIDGIGATEMIHIFISAKGDEIRPGATGKPLPGYQACVLDEHGQPLQPPNA
                     GRLAVKGPSGCRYLDDPRQLEYVRNGWNITGDRYYVDEDGYFWFQARADDMIISGGYN
                     IAGPEVEAALLQHPAVRECAVVGARDEQRGQIVKAFVVTNEGHAAGEALARELQDHVK
                     RSIAPYKYPRAVEFVPELPKTPTGKLQRYVLREGRAASPRT"
     gene            1893..>2266
                     /locus_tag="VFY80_00110"
     CDS             1893..>2266
                     /locus_tag="VFY80_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6006329.1"
                     /translation="MRQIPKSSCPWLKLPRSGSGLNVEDFLTFRMNRLSNALRTNLTK
                     AYLEDFGLSLPEWRLLALVARFSPLRFSEVTARSGMDKGQVSRTLRVMAKRGLTKMKV
                     IRDRGSRSAEALAAPVMVSITA"
BASE COUNT          381 a          739 c          752 g          394 t
ORIGIN      
        1 ttcggcttca cgccgctgta cacgcgctac gaagacgtat ggcgggcggt cgagatcctg
       61 cgggacgtgc tgcagacgca gtgctggtcg gactcccgct atgcggtaag agctgccgtg
      121 acatgaacgc gcacttttcg ccgacggacg gcgcgtacac ggcgcacgtc gacacttttg
      181 cacgcgaccg cctgccgccg cgcgagcagt ggccggagtt ccgcttcgat ctgcccgagc
      241 tgcgatatcc gccgcggctg aactgcgcct ccgtgctgct cgacgaagcc attctcgaag
      301 ggcatggcgc acgggtggcg ctgtattcgc agggcggcaa ctggtcttat ttcgaattgc
      361 tggagcgtgc caatcgcatc gccaacgtgc tggtgcgcga tctcggtgtg gtgcccggca
      421 accgcgtgct gctgcgtggc gccaacacgc cgatgctggt cgcaagctgg ctggccgtga
      481 tgaaggtggg cgcgatcgcg gtgaccacga tgccgatgct ccgggccagg gaactcatcg
      541 tcatggcgga gcgggcgcag gtcgaccacg cgctttgcga agcgcggctc gccggtgagc
      601 tccgcgaggc tgcgcaagcc actcgtcggc tgtcgcgtgt cgcaacctac ggcgacggac
      661 atctcgaagg cctgatggcg cagcgcacgg acgagttccg taacgtcgca actgccgcgg
      721 aggatgtcag cctgctcgct tttacctccg gtacgaccgg caacccgaaa gcgacgatgc
      781 actttcatcg tgacgttctc gtaatgcgcg acgtcgtggg ccgatatttg ctggagacct
      841 cgcccgacga catctacacg ggcagtccgc cgctcggctt cacgttcggc ctgggagcat
      901 tgctcgtgtt cccgctgggc ggccgcggcg cggtcgcgct cgttgagcaa ccaacgcccg
      961 acaacctgct ggacaccatc gcgcgctttc gggcaactgc cctgttcacg gcgccgaccg
     1021 catatcgcgc actcttgccg ctcgtgcccg ggcgcgacct tgcgagcctg cgccgctgcg
     1081 tctctgcggg tgaagcgctg ccgaaggcga catccgacgc gtggcacgcg gccaccgggc
     1141 tgcgcatcat cgatggcatc ggcgccaccg agatgatcca catcttcatc tcggcgaagg
     1201 gcgacgagat ccggcccggc gcgacgggca agccgctgcc ggggtaccag gcctgcgtgc
     1261 tcgacgagca cgggcagccg ctgcagccgc cgaatgcggg gcgcctcgcg gtgaaaggtc
     1321 cgagcggctg tcgctacctc gacgatccgc gccagctcga gtacgtgcgc aacggctgga
     1381 acatcacggg cgatcgctac tacgtcgacg aggacggcta cttctggttc caggcgcgcg
     1441 ccgacgacat gatcatctcg ggcggctaca acatcgccgg cccggaggtc gaggccgcgt
     1501 tgctgcaaca tccggcggtg cgcgagtgtg ccgtggtcgg cgcgcgcgac gagcagcggg
     1561 gccagatcgt caaggccttc gtcgtaacca acgaaggtca cgcggccggc gaggcgctcg
     1621 cgcgcgaact gcaggatcac gtcaagcgct cgatcgctcc gtacaaatac ccacgcgccg
     1681 tggaattcgt accggagttg ccaaaaacgc ccacgggcaa gctgcagcga tacgtattgc
     1741 gcgaaggccg cgcagcttcg ccgcggacct gacacttctt attgttctca tcggggactc
     1801 gttgcggcag aatccgcaga ccctgatcat tgtcggcgca acggcgccgt tggaatctcc
     1861 ctgtgaacag aattcagcaa cagaagccgc gcatgcggca gatcccgaaa tcttcctgtc
     1921 cgtggctgaa gctgccgcgc agcggctcag gcctcaacgt ggaggacttc ctgactttcc
     1981 gcatgaatcg cctgtcgaat gcgctgcgta cgaatctgac gaaagcgtat ctggaagatt
     2041 tcggcctcag cctgccggag tggcgattgc tcgcgctggt tgctcgcttt tccccattgc
     2101 gattttccga agtcaccgcg cgcagcggca tggacaaggg gcaggtgagc cgcacgctgc
     2161 gcgtgatggc caagcgcggg ctgacgaaga tgaaggtgat ccgcgaccgc ggctcgcgct
     2221 cggccgaggc gcttgctgct ccggtgatgg tgtcgatcac ggcgaa
//
LOCUS       DASURG010000007         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_717155, whole genome shotgun sequence.
ACCESSION   DASURG010000007 DASURG010000000
VERSION     DASURG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717155"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFY80_00115"
     CDS             complement(<1..203)
                     /locus_tag="VFY80_00115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEX6006330.1"
                     /translation="MTAGKTLARGLSALRIDLASEAQARLMQYAALIQKWNRTYNLTA
                     VQEEEGIVIHHLLDSLAVSPHIGA"
     gene            complement(200..2089)
                     /gene="mnmG"
                     /locus_tag="VFY80_00120"
     CDS             complement(200..2089)
                     /gene="mnmG"
                     /locus_tag="VFY80_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX6006331.1"
                     /translation="MFDSYDVIVVGGGHAGTEAALAAARMGCRTLLLTHSVETLGQMS
                     CNPSIGGIGKGHLVKEIDALDGVMAAVADESGIQFRILNSRKGPAVRATRAQADRALY
                     RRAVRTRLENEPRLRIFQQAVDDLILEGDRVCGVVTQTGVRVRARSVVLTTGTFLSGL
                     IHVGLCNYQAGRAGDPPAVSLAARLRELRLPVGRLKTGTPPRIDGRSIDFSVIEAQPG
                     DEPTPVFSFLGTPDQHPRQLPCWITHTNARTHEIIRGALDRSPMFTGVIEGVGPRYCP
                     SIEDKVHRFADKAAHQIFLEPEGLTVNEFYPNGISTSLPYDVQHALVRSIRGLERAHI
                     TRPGYAIEYDYFDPRGLRGSLETKAIGGLYFAGQINGTTGYEEAAAQGLLAGINAACA
                     VLGRDPWSPRRNEAYLGVLVDDLITRGVSEPYRMFTSRAEYRLSLREDNADLRLTEIG
                     RKLGVVGDGRWAAFARKRDAIAREQERLKSTWINPRMVAAEHAKRVLGRVIEREYSLA
                     ELLRRPDVTYAQLMTLPSAAGGEQVDAQVAEQLEIQAKYRGYVERQQEEVERAAQYES
                     VPLPRDLDYRMLRGLSVEVQQKLNQHRPETIGQAARVSGVTPAAISVLLVHARRGFGN
                     SKKTA"
     gene            complement(2197..>2710)
                     /locus_tag="VFY80_00125"
     CDS             complement(2197..>2710)
                     /locus_tag="VFY80_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX6006332.1"
                     /translation="DQVEALGIGRTWRAIEEADAMVLVVDATKGETDAEHAIEARLPP
                     TQRRIVVMNKIDLIPKPPALVKEQTNRFKVWLSAKTGEGLDYLRRALLESAGWHGSSE
                     GLFLARTRHLEALRLAQSHVRQANEQVERPEFLAEELRLAHEALQSILGEFTADDLLG
                     EIFGRFCIGK"
BASE COUNT          486 a          894 c          801 g          529 t
ORIGIN      
        1 gcgccgatat ggggcgacac cgcaaggctg tcgaggagat gatggattac gatgccttct
       61 tcctcctgaa ccgcggtcag gttgtacgtc cggttccact tctgaatgag cgccgcatac
      121 tgcatgagac gcgcctgcgc ctcggatgca agatcgattc gtaatgcgct cagtccccgt
      181 gccagcgttt tgccggcagt catgcggttt tcttgctatt accgaatccg cgtcgagcgt
      241 gcacgagcag caccgagatg gcggccggcg tcacgcctga aacgcgcgca gcctgtccta
      301 tcgtttcggg ccggtgctga ttgagctttt gctgcacctc gacggaaagc ccgcgcagca
      361 tcctgtagtc gagatcgcgc ggcagcggga cactttcgta ctgtgcagcg cgctcgactt
      421 cttcctgctg gcgctcgaca tatccgcggt acttcgcctg aatctcgagc tgctcagcaa
      481 cttgcgcatc gacctgctcg ccgcctgccg cgcttggaag cgtcatcagc tgcgcatagg
      541 tcacgtcggg tcgccgcagc agttcggcta gcgaatactc gcgttcgatc acgcgtccca
      601 ggactcgttt cgcatgttct gcggccacca tcctgggatt gatccaggtg gacttcagcc
      661 gctcctgctc gcgcgcgatg gcatcgcgct tgcgcgcgaa cgccgcccag cgaccgtccc
      721 ccacgacgcc gagcttgcgc ccgatttcgg tcaacctcag atcggcattg tcttcgcgca
      781 gcgaaagccg atactccgcg cggctggtaa acatgcggta cggctccgac acaccgcgcg
      841 tgatcaaatc gtccaccaac acgccgagat acgcctcgtt gcggcgcgga gaccatggat
      901 cgcgccctag cacggcgcac gccgcgttga tccctgcgag caatccctgc gcggccgcct
      961 cctcgtaacc tgtcgtgccg ttgatctgcc ccgcgaaata gagcccgccg atcgccttcg
     1021 tctcgagcga cccgcggagg ccgcgcggat cgaaatagtc gtactcgatc gcgtagccgg
     1081 gacgcgtgat gtgtgcccgt tcgagaccgc gtatcgagcg taccagcgca tgctgtacat
     1141 cgtacggcag gctggtcgat atgccattgg gataaaactc attgaccgtc agcccttcgg
     1201 gctcgaggaa gatctggtgc gcggccttgt ccgcgaaacg atgcaccttg tcctcgatcg
     1261 agggacagta gcgtggtccg acgccttcga tgactccggt gaacatcggt gagcgatcga
     1321 gtgcgccgcg gatgatttcg tgcgtgcgcg cattcgtatg ggtaatccag cacggcagtt
     1381 gtcgcggatg ctgatcgggt gtgccaagaa aagaaaacac cggagtgggc tcatcgcccg
     1441 gctgcgcctc gataaccgaa aaatcaatgc tgcggccgtc gatgcggggc ggcgtgccgg
     1501 tcttgagccg cccgaccggc agcctcaact cgcgcaaacg cgctgccagg ctgaccgcgg
     1561 gagggtcgcc ggcgcgtccc gcctggtaat tgcacagccc gacgtggatc aggccggaaa
     1621 gaaacgttcc ggtcgtcagc acaacgctac gcgctctgac acgcactccg gtctgcgtca
     1681 ctacgccgca gacccgatcg ccttcgagga ttaggtcgtc tacggcctgc tggaagatgc
     1741 gcagtcgggg ctcgttctcg agccgcgtac gcaccgcgcg gcgatagagg gcgcggtccg
     1801 cctgtgcccg cgtggcgcga actgcaggac ccttgcgcga gttcagtata cgaaactgga
     1861 tgcccgattc gtccgcaaca gccgccatga caccatcgag cgcatcgatc tccttgacga
     1921 gatgcccttt tccgatgccg ccgatcgacg ggttgcacga catctggccc agtgtttcga
     1981 cgctgtgcgt gagaagcaga gtacgacaac ccattcgggc agcggcgagc gctgcctcgg
     2041 tgccggcgtg accgccaccc accacaataa cgtcatagct atcaaacact tgtctcaacg
     2101 cgcccttctg atgccactcc agcgcgcatg ataaacgatt ttgcgagcaa tgggcggagg
     2161 ttccacgtgg aacaacaatt cggtttcacg tgaaactcac ttccctatgc agaatcggcc
     2221 gaatatctcg cccaataaat cgtccgcggt gaactcccct aagatagatt ggagcgcctc
     2281 gtgcgccagg cgcagctcct ctgccaagaa ctctgggcgc tcgacttgct cgttcgcttg
     2341 acgcacatgg ctttgcgcga gacgcagggc ctcgagatgc cgggtgcgcg cgaggaacag
     2401 cccctccgac gatccatgcc agcccgcgct ctccaagaga gctcgacgca agtaatccaa
     2461 accctctccc gtcttggcgg acaaccatac cttgaaccgg ttcgtttgct ccttcactag
     2521 ggcgggcggc tttgggatca gatcgatctt gttcatcacg acgatgcgtc gctgtgtcgg
     2581 ggggagccgc gcctcgatcg cgtgctccgc gtccgtctcg cccttcgttg cgtcgaccac
     2641 cagcaccatg gcatccgcct cttcgatggc gcgccacgtt cgtccgatgc ccagcgcctc
     2701 cacttggtcc
//
LOCUS       DASURG010000008         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_309436, whole genome shotgun sequence.
ACCESSION   DASURG010000008 DASURG010000000
VERSION     DASURG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309436"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFY80_00130"
     CDS             complement(<1..192)
                     /locus_tag="VFY80_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6006333.1"
                     /translation="MKIATWNVNGIRARQGEFCAWLERERPDVVCLQELKAERAQVPP
                     QCAHDDYHVYWHGLRAYSGV"
     gene            complement(216..896)
                     /locus_tag="VFY80_00135"
     CDS             complement(216..896)
                     /locus_tag="VFY80_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX6006334.1"
                     /translation="MRRILVFLTCAVSLMHAHAAGLDSLTNADASMGLKQALTDAAAI
                     AVGRLGVENGYLGNPKVKIPLPPSLQRVEGAMRVMGMRRQADELVVAMNRAAEAAAPE
                     AKALLIDAVKKMTVQDAKQILTGSDTAATEYFRRTTRPQLTERFLPIVKKATDRVGLA
                     QQYNSIAGQGAALGLVREDQATIERYVTAKALDGLYLMIGEQERAFRQNPLGASSDIV
                     KKVFGALR"
     gene            895..1437
                     /locus_tag="VFY80_00140"
     CDS             895..1437
                     /locus_tag="VFY80_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6006335.1"
                     /translation="MVFSPRKWRSAGGEAKLRRWILLAIAIAAGALGYALVSRPETAP
                     AVAFPTLRGESLALNALRGKVVLIEFWATDCATCVKEMPQLVKTYERYRGRGFEAIAV
                     AMRYDPPNYVIAYTERNRLPFPVAFDPTGEIAKAFSGVKFTPTTFLVDKRGRIVARLI
                     GERDFARLNALIEEVLEEPA"
     gene            complement(1515..2435)
                     /gene="lipA"
                     /locus_tag="VFY80_00145"
                     /pseudo
     CDS             complement(1515..2435)
                     /gene="lipA"
                     /locus_tag="VFY80_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            complement(2450..3109)
                     /gene="lipB"
                     /locus_tag="VFY80_00150"
     CDS             complement(2450..3109)
                     /gene="lipB"
                     /locus_tag="VFY80_00150"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX6006336.1"
                     /translation="MNTAPATARRETALSEIPQVNVRRLGLVDYEPTWRAMQSYTAQR
                     DNAAADEIWLLQHRPVYTVGLAGRPEHLPRGANGIPVIRTDRGGQITYHGPGQAIAYL
                     LLDMRRRGLSIRPLVRRMEQAVIDLLARAGVDAHRREDAPGVYVAEAKVAALGLRIRR
                     GCCYHGIALNIDMDLTPFRAINPCGYPGLPITQMRDLGIAHDVSQVGSDLVRHLMEVL
                     Q"
     gene            3176..3679
                     /locus_tag="VFY80_00155"
     CDS             3176..3679
                     /locus_tag="VFY80_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006337.1"
                     /translation="MQNAFRLLMSVPLCAAVALMLWMASARAQPAEDTMASLAVLYNE
                     RHRLVAFKDVCSRVLPKLRRETQAAYQEWVDRHEEVLENLEARFLVMVKKVSRNQTEY
                     NENYRKYRGIVEEERQAQKNAFLAMPKEELLKECKEYPAYLRGESSDMPSRYPEEFAA
                     LYGRKKP"
     gene            3818..3934
                     /locus_tag="VFY80_00160"
     CDS             3818..3934
                     /locus_tag="VFY80_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEX6006338.1"
                     /translation="MGKGDRRTRRGKIFKGSYGKSRPHKNRTRKKKSVPKRA"
     gene            complement(3980..4243)
                     /locus_tag="VFY80_00165"
     CDS             complement(3980..4243)
                     /locus_tag="VFY80_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX6006339.1"
                     /translation="MTEASLIEYPCDFPIKILGETRAGFAQAVLEVVQRHAPDFDGAS
                     MEMKASRRGKYLSVTCVIRATSRAQLDALYRDLCDHPMVVMVL"
     gene            complement(4240..>4383)
                     /locus_tag="VFY80_00170"
     CDS             complement(4240..>4383)
                     /locus_tag="VFY80_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEX6006340.1"
                     /translation="VTSSTKEVLAVTTLDGKAVGAGKPGVVFRQVHALYQEFKRKVMR
                     RAA"
BASE COUNT          806 a         1405 c         1396 g          776 t
ORIGIN      
        1 gacgccggaa tacgcacgaa gtccgtgcca gtacacgtgg tagtcatcat gcgcgcattg
       61 cgggggcacc tgtgcgcgct cggccttgag ctcctggagg cagacgacgt ccgggcgctc
      121 ccgctcgagc cacgcgcaga attccccctg tctcgcgcgg atgccgttga cgttccacgt
      181 agcgatcttc atcgatcact tcatccgccg gcccgtcagc gcagcgcgcc gaagactttc
      241 ttcacgatat cgctcgatgc cccgagcgga ttttgccgga aggcgcgttc ctgctcgccg
      301 atcatcagat acaggccgtc gagcgctttc gccgtcacgt aacgctcgat ggtcgcctga
      361 tcttccctca cgagcccgag cgccgcgccc tgccccgcga tcgagttgta ctgctgcgcg
      421 agcccgacgc gatcggtcgc tttcttcacg atgggcagga agcgctcggt cagctgcggc
      481 cgggtcgtac gccggaaata ttcggtcgcc gcggtatcgc tgcccgtcag tatctgcttt
      541 gcgtcctgca ccgtcatttt cttcactgcg tcgatcagga gcgctttcgc ttcgggggcc
      601 gccgcctcag cggcccgatt cattgcgacg accaactcgt cggcctgccg gcgcatgccc
      661 atgacgcgca tggcaccttc gacccgctgc agcgacggcg ggaggggaat cttcaccttg
      721 ggattgccga gataaccgtt ctcgacgccg agacggccga ccgcgattgc ggctgcgtcc
      781 gtcagcgcct gcttcaatcc catcgaagcg tcggcattcg tgaggctgtc gagtcctgcg
      841 gcatgcgcgt gcatgaggct gacagcgcac gtcaaaaaaa cgagaatacg gcgcatggtg
      901 ttctccccta gaaaatggcg ctcagcgggt ggtgaagcga agctgcgcag gtggattcta
      961 ctcgcaatcg cgatcgccgc cggcgcgctg ggctacgcgc tcgtgtctcg ccccgagacc
     1021 gccccggcag tggcgttccc gacgctcaga ggggaaagcc tcgcgctcaa cgcgttgcgc
     1081 ggcaaggtgg tgctcatcga attctgggcg accgactgcg cgacgtgcgt caaggaaatg
     1141 ccgcagctcg tgaagacgta cgagcgctat cgcgggcgcg gattcgaggc gatcgccgtt
     1201 gcgatgcgct acgatccgcc caattatgtc atcgcttaca ccgagcgcaa tcgcctgccg
     1261 ttccccgtcg cattcgaccc gaccggggaa atcgcaaagg cgttcagcgg cgtgaaattc
     1321 acgcccacga cattcctcgt ggacaagcgc ggtaggatcg tggcccggct cataggcgag
     1381 cgcgacttcg cacggctcaa tgcgctgatc gaagaagtat tggaagagcc ggcgtgatga
     1441 cccgtgcagc gcgccggtgc aggcaagact cgcttgttac gcgagtcgcg ggaggaaacg
     1501 ggttagcgag cgcttcagac cacgccagct tcgtgcgcct ggcggtcggc atggtagctc
     1561 gagcgcacga gcggcccgca tgccgcgtgc gtaaatccca tcgcgtcggc ctgcctgctg
     1621 aaaaagtcga acaccgaagg ttctacgtag cgcagcacgg gcagatggtg ttcgctcggc
     1681 tgcaggtact ggccgatggt cagcatgtcc acgccgtgcg cacgcaagtc gcgcatgacg
     1741 gcaaggattt cctcgtccgt ttcgccgagt ccgaccataa ggccggactt ggtcgggagt
     1801 gcgggaaagc gggccttgaa ggacttgagc agcctgagag agtgagcata gtcggcgccc
     1861 gggcgcgcct gccggtagag gcgcggcacc gtttcgagat tgtggttcat cacgtcgggc
     1921 gggcacgtcg cgagcgcatc gagcgcaatc ccgaggcggc cgcggaaatc cggcaccaag
     1981 gtctcgattc tcgtctgtgg agagttcgcg cggaccgcat cgatgcaccg ggcgaagtgc
     2041 tgggcaccgc cgtcacgcaa atcgtcgcga tcgacgctcg tgatcacgac gtacttcagg
     2101 cgcagcgccg cgatggtgcg cgcaagattg agcggctctg acgcatcggg cgggagcggt
     2161 ttcccgtgcg cgacatcgca gaacgggcag cgccgcgtgc aaagctcgcc gaggatcatg
     2221 aaagtcgcgg tgcccttccc gaagcactcg ccgatgttgg gacacgacgc ctcctcgcac
     2281 acggtatgca gcgtgtgctc gcgcaggatg cgcttcacct cgtggaaacg ctggccgtcg
     2341 cccaagcgga cgcgtatcca gtccggcttc ttcaggcgtt ccgcgggcac gattttgaca
     2401 ggaatgcgcg ccgtcttggc ttcccccttc tgcttttcgc ccgccgcgct cactgaagca
     2461 cctccatcag atgccggacg agatcggacc ccacctgact cacgtcgtgc gcgataccaa
     2521 gatcacgcat ctgcgtgatg ggcaggccgg gatagccgca gggattgatc gcgcgaaacg
     2581 gcgtgagatc catgtcgatg ttgagggcga tgccgtgata gcagcagccg cggcggatgc
     2641 gcagcccgag tgctgcgacc ttcgcttcgg cgacgtacac cccgggcgca tcttcgcgcc
     2701 tgtgcgcgtc gacgccggcc cgagcgagca agtctatcac ggcctgttcc atgcgccgca
     2761 cgagcggccg aatgctcagc ccgcggcgcc gcatgtcgag cagcaggtat gcgatcgcct
     2821 gtcccgggcc atgataggtg atctgcccgc cacggtcggt acgaatgacc ggaatgccat
     2881 ttgcgccgcg cggcagatgt tccggcctgc cggcgagacc gaccgtatag acggggcgat
     2941 gctgcagcag ccagatttca tccgcggcag cattgtcgcg ctgcgcggtg tacgactgca
     3001 tggcacgcca cgtcggttcg taatccacga ggccgagacg ccgcacgttc acctgtggaa
     3061 tctcgctcag ggccgtctcg cgccgagcgg tagcgggagc ggtgttcatt gccgttgttc
     3121 gatcataaga acagtagcac aatgccgcaa cccgcgcgcc cgcagtcgag tttccatgca
     3181 aaacgcattc cgtctcctca tgtctgtccc gctttgcgca gcggtcgcgc tcatgctctg
     3241 gatggcttca gctcgcgcgc agccggccga ggacaccatg gcgagtctgg ccgtcctcta
     3301 caacgagcgg caccgtctcg ttgcgttcaa ggacgtatgc agccgggtgc ttcccaagtt
     3361 gcggcgcgaa acgcaggcgg cgtaccaaga gtgggtcgac cggcatgaag aggtactgga
     3421 aaacctcgag gcgcggtttc tcgtcatggt gaagaaggtg tcgcgcaatc agacggaata
     3481 caacgagaac taccgcaagt atcgcggcat cgtcgaagag gagcggcaag cgcagaagaa
     3541 cgcgttcctc gcgatgccca aggaggaact gctcaaggaa tgcaaggagt atcccgctta
     3601 cctgcgcggt gagagctccg acatgccgag tcgctacccc gaggagttcg ccgcgctcta
     3661 cggacgaaaa aagccgtagc cgctcggagg agcggtatat cgcatcctct cgtcggactg
     3721 tcgttgcacg ccttgcccgc cgacttcccc gcgcggagcc gcttccgcgc gtcatgtaca
     3781 atcggtcacg ccggctgatc attcaaggag gacaaccatg ggcaaaggcg acagacgtac
     3841 gcgacgcggc aaaatcttca agggctcgta cggcaagtcg cggccgcaca agaatcgcac
     3901 caggaaaaag aaaagcgtgc ccaaacgcgc ttgaccgcgt tgcggatgcc ctgcgcagcc
     3961 gggggggcta ttcagcgcac tagagcacca tgaccaccat gggatggtcg cagaggtcgc
     4021 gataaagcgc atcgagctgc gcgcgcgagg tcgcccggat cacgcaggtc acgctcaggt
     4081 acttgccgcg cctgctcgcc ttcatctcca tcgatgcgcc atcgaaatcc ggagcatgac
     4141 gctgaaccac ctccagcacc gcctgtgcga agccggcacg ggtctcgccg aggattttga
     4201 tcgggaagtc gcagggatat tcgatgagcg aagcttcggt catgccgctc gtcgcatcac
     4261 cttccgcttg aattcctgat agagggcgtg cacctgccgg aacaccacgc caggcttacc
     4321 ggcgccgacc gctttgccgt cgagagtcgt caccgcgagc acttccttag tggaggacgt
     4381 cac
//
LOCUS       DASURG010000009         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_615715, whole genome shotgun sequence.
ACCESSION   DASURG010000009 DASURG010000000
VERSION     DASURG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615715"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..734
                     /locus_tag="VFY80_00175"
     CDS             21..734
                     /locus_tag="VFY80_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6006341.1"
                     /translation="MPEYSPKRRTALVFAGSGTSGAYHAGVLKALEEAGTHSAEITEF
                     LPIESVDPLFFDKAYYLAPDKGGAKPYALLARALRDSRRCALGRWAARGKQYIVMIRP
                     VENGLVMQQLLYAAEVRSIKDIEIPETEVRDAELKLAQQLIEQQATDTFDPTAYKDEV
                     RERIEAAVQKKVEGQEITLAEAPETGGAQVIDLMEALRASLGKKAPAKAPEPRAATRK
                     PPKRAAQQAEPPARKRARK"
     gene            759..1574
                     /locus_tag="VFY80_00180"
     CDS             759..1574
                     /locus_tag="VFY80_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6006342.1"
                     /translation="MHQYGVRDVERLLRLPRSTIRALTEAGFVSPARGPRNAWRFSFQ
                     DLIVLRTAQALVEADVPRRRITRAVRALRRRLPEAMPFSGLSICAVGGRVVVKEGAQH
                     WQADSGQYLLSFEGDPQTGMLSVIAPRAESVPARAAEDWFKQGLALENMDAEAALAAY
                     EKAIAADRARLDARINCGRLLHEIGRHREAEQMYRDAIEDCGAEAILLYNLGVLLEDM
                     SRKTDAAEAYEAALRVDPDLADCHYNLALLCGELGRAKDAIRHMARYRKLASN"
     gene            1599..2708
                     /locus_tag="VFY80_00185"
     CDS             1599..2708
                     /locus_tag="VFY80_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX6006343.1"
                     /translation="MKTKAAIAYEPGKPLEIATLDLDGPRAGEVVIEIRASGVCHTDE
                     FTRSGADPEGLFPVVFGHEGAGVVVDVGSGVTTVRKDDHVIPLYIPECRQCKACLSRK
                     TNLCTAIRTTQGKGVMPDGTSRFSLDGRMIHHYMGCSTFSHYTVLPEIAVAKIREDAP
                     FEKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQGARMAGANRIIGVD
                     INPRRKPLAEKFGMTDFVNPKEVGGDLVQYLITLTDGGADYSFECVGNVDLMRQALEC
                     CHRGWGVSVIIGVAGAGQEIRTRPFQLVTGRVWKGTAFGGARGRTDVPRIVDWYMDGR
                     INIDDLITHVMPIERINEAFDLMHAGESIRSVVTF"
BASE COUNT          530 a          894 c          951 g          450 t
ORIGIN      
        1 cggtgataac ttaaggcctc atgcccgagt actcgccgaa gcggcggacc gcgctggtct
       61 tcgcgggaag cggcacgtcc ggggcgtatc acgccggcgt gctcaaggcg ctcgaggagg
      121 ccggcaccca cagcgccgag atcacggaat tcctcccgat cgaatcggtc gatccacttt
      181 tcttcgacaa ggcctactac ctcgctccgg acaagggcgg tgccaagccc tacgcgctgc
      241 tggcgagggc gctgcgcgac tccaggcgct gcgcgctcgg ccgctgggcg gcgcgcggca
      301 agcagtacat cgtgatgatc cggccggtcg agaacggcct ggtgatgcag cagctgctct
      361 acgccgcgga agtgcgatcg atcaaggaca tcgaaatccc cgagaccgag gtcagggacg
      421 ccgagctcaa gctcgcgcag cagctgatcg agcagcaggc gaccgacacc tttgatccga
      481 ccgcgtacaa ggatgaagtg cgcgagcgga tcgaggctgc ggtgcagaag aaggtcgaag
      541 ggcaggagat cacgctcgcc gaagcccccg agacgggcgg tgctcaggtc atcgatctca
      601 tggaggcact ccgcgcgagc ctcggcaaaa aagcgcccgc gaaggcgccg gagccgcgcg
      661 ccgcgacgcg caagccgccg aagcgcgcag cacagcaggc agagccgccc gcgcgcaagc
      721 gcgcgaggaa gtaacgcacc acgcagcgcg gcaacgccat gcaccagtac ggcgtgcgcg
      781 acgtcgagcg gttgctccgg cttccccgca gcaccatacg cgcactgacc gaggccggct
      841 tcgtctcgcc cgcacgcggc ccgcgcaacg cgtggcgctt ttcatttcag gacctgatcg
      901 tgctacgcac ggctcaggca ctcgtggaag ccgatgtgcc gcgcaggcgc atcacgcgcg
      961 ccgtcagggc gttgcgccgc cgcctgcccg aggcgatgcc cttctcgggc ttgagcatct
     1021 gcgcagtggg cggccgggtc gtggtgaagg aaggcgcgca gcactggcag gcggactcgg
     1081 ggcagtatct cctctccttt gaaggcgatc ctcagaccgg aatgctgagc gtgatcgcgc
     1141 cgcgcgccga gtcggtgccc gcacgcgccg ccgaggactg gttcaagcag ggccttgcgc
     1201 tcgagaatat ggacgcggag gccgcgctcg cggcttacga gaaagcgatc gcagccgatc
     1261 gagcgcgcct cgacgcccgc atcaattgcg ggcgcctgct ccacgaaatc ggccggcacc
     1321 gcgaggcgga gcagatgtat cgcgatgcga tcgaggactg cggcgcagag gcgatcctgc
     1381 tctacaatct tggcgtgctg ctcgaggaca tgtcgcgcaa gacggacgcg gcggaggcct
     1441 atgaagctgc gctgcgcgtc gatcccgatc tcgcggattg ccattacaac ctcgcgctgc
     1501 tctgcggcga gctcggccgc gcaaaggacg cgatccgtca catggcgcgg taccggaaac
     1561 tcgcctcgaa ctgacgatcc caatccgagg agcgctcgat gaagaccaaa gccgcgatcg
     1621 cttacgagcc gggcaagccg ctcgaaattg cgacgctcga tctcgacgga ccgagagccg
     1681 gcgaggtggt gattgaaatc cgggcatcgg gcgtgtgcca cacggacgag ttcacgcgtt
     1741 ccggcgccga ccccgaaggc ctctttccgg ttgttttcgg acacgaaggg gccggtgtgg
     1801 tggtcgatgt cggttccggt gtcaccacgg tcaggaagga cgaccacgtc attccgctct
     1861 acattcccga gtgccggcaa tgcaaggcgt gcctctcgcg caagaccaat ctctgcacgg
     1921 cgatccgcac gacacagggc aagggtgtca tgcccgacgg gacgagccgg ttctcgctcg
     1981 acggccgcat gattcatcac tacatgggat gctcgacgtt ctcccattac accgtgctgc
     2041 ccgaaatcgc cgtggcgaaa atccgtgagg acgcaccatt cgagaaagtg tgttacatcg
     2101 gatgtggcgt gacgaccggc atcggcgctg tcatcaatac cgcaaaggtc gagccgggtg
     2161 ccaacgtggt ggtgttcggt ctaggcggta tcggcttgaa cgtcatccag ggcgcacgca
     2221 tggccggcgc aaacaggatt atcggcgtgg acatcaatcc gcggcgcaaa ccgcttgccg
     2281 aaaagttcgg catgacggac ttcgtcaatc ccaaggaagt cggaggcgat ctcgtccagt
     2341 acctcatcac gctcaccgat ggcggcgcgg attacagctt cgaatgcgtc ggaaacgtcg
     2401 atctcatgcg ccaggcgctc gaatgctgcc atcgcggctg gggcgtgtcg gtgatcatcg
     2461 gtgtggcggg tgcgggtcag gaaatccgca cgcgaccgtt ccagctcgtg acgggccggg
     2521 tgtggaaggg cacggcgttc ggcggcgcgc gcgggcgcac ggacgtgccg agaatcgtcg
     2581 attggtacat ggatggacgg atcaacatcg acgatctcat cacgcacgtc atgccgatcg
     2641 agcgcatcaa cgaggcgttc gatctcatgc acgccggcga gtcgatccgt tcggtcgtga
     2701 cgttctgaat cgcattcgtc ttctcggcga aagccggcga tccaggtaag aattctggat
     2761 tccggcgtat gccggaatga cgtggctctc ggcgtacgcg ggagtggcgg cgcgctcggc
     2821 gtacg
//
LOCUS       DASURG010000010         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1331055, whole genome shotgun sequence.
ACCESSION   DASURG010000010 DASURG010000000
VERSION     DASURG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331055"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFY80_00190"
     CDS             <1..262
                     /locus_tag="VFY80_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEX6006344.1"
                     /translation="MLALSTYLPLAETVTQEVHAANARTPIFMAHGSDDQVIPLETGE
                     GSRDMLKRMGYEVEWREYPMPHSVCMEEIRDIAAWMRRVLAD"
     gene            complement(302..1825)
                     /locus_tag="VFY80_00195"
     CDS             complement(302..1825)
                     /locus_tag="VFY80_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006345.1"
                     /translation="MISWFGRAKAEQPASESARLQAFVASLPADDSAEALQQITRELQ
                     ALRSNDELKLDHRFRNLDLLDGASRSHERALLLEYLATPRHKKSHEQRLWNSAYGFWR
                     ELGAEYQRCVREGGKGGSAGKSTVPIFVGRALRTLRQQLRWSLLRYESAEPRIWTEIA
                     RLYQHAEEHGFDDEAIAVYPGSSGSGSSKQELLKALMLAASSTDSLLPSSQHLATRLV
                     AYFAGFFVISPRPDDGCTHWIDLAAPNAPVRLVREPPPGKGLRYFGAGRGLHELEQLR
                     AHVAYTRTLPEGLDLNGNYDDEVVLALLKHLEQDWAGKSQSRRYERRKIASRVTVVPG
                     LKEIIGSLEFAHNDSLDFTHRQAAESWIVEDMSEGGYGAVIPSVAGDWVEVGSLIGFE
                     GETFRDWRIGVIRRVMRTEQQQRVGVELLAQTSTLVRLRRPGAAGSPAPRDAVLFSRK
                     LEAKKNVKIALAKDMFDGKENVEMLIGDETYLLRPEELVERGPHYDVIRYAILRAVH"
     gene            complement(1883..>2997)
                     /locus_tag="VFY80_00200"
     CDS             complement(1883..>2997)
                     /locus_tag="VFY80_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006346.1"
                     /translation="MASAPALGAYADARANKKTLLALSTAGCVLFTAALALTDRGDVV
                     LAFMLVAMSSYFFGCGENLIAAFLPELGRERGLGRISGWGWSLGYVGGLVSLAASLAY
                     VIAAQERGAAATEFVPVTMLITASIFALASVPTFLFLRERAIRQEDRPAHAVVTAFSR
                     LFRTVHHARRYSDFGRFLICLVFYQAGIQAVVALAAIYAQQAMGFTTRDTLVLVLVVN
                     VTAALGAFFFGHVQDRIGHVPTIMATLVGWLVTVVVAWAAEDRTLFWIAANLAGLCLG
                     SSQSAGRALVGYLSPPDRIAEFFGLWGLAVKLSSILGPVTYGAVTWITNGDHRTAILV
                     TGTYFVIGMAIVATVDPARGRRAAISAQGDGSPCAR"
BASE COUNT          566 a          995 c          948 g          488 t
ORIGIN      
        1 gatgctcgcg ctgtcgactt acctgccgct ggcggagaca gtgacgcagg aagtgcacgc
       61 cgcgaatgcg cgcacgccga tcttcatggc acacggcagc gacgatcagg tgattccgct
      121 cgaaacgggc gaggggtcgc gcgacatgct aaagcgcatg ggctacgagg tggagtggcg
      181 cgaatacccg atgccgcatt ccgtctgcat ggaggaaatc cgggacattg ccgcctggat
      241 gagacgggtg ctcgcagact gacacaagcg actcgccgca cccgcgacgg gtggctgggt
      301 atcagtgcac cgcgcgcaga attgcgtaac gaatgacgtc gtaatgcggc ccgcgctcga
      361 ccaactcttc cggcctcagg agataggtct catccccgat cagcatctcg acattctcct
      421 tgccgtcgaa catatccttt gcaagcgcga tcttgacgtt cttcttcgcc tcgagcttgc
      481 gagaaaatag cactgcatct cgtggcgccg gtgaacccgc ggcgccgggc cggcgtaacc
      541 gcaccagcgt cgaggtttgc gcaagcagtt ccacgccgac tcgctgctgc tgctcggtgc
      601 gcatgacgcg tcggattacg ccgatacgcc agtcgcgaaa cgtctcgcct tcgaagccga
      661 tcaggctgcc tacttccacc caatcgccgg caacagacgg tatcaccgcc ccatagccgc
      721 cctcgctcat gtcttccacg atccagctct ccgcggcctg gcggtgggtg aaatcgagcg
      781 agtcgttgtg ggcgaattcg agcgagccga tgatttcctt gagtcccggc acaacggtca
      841 cccggctcgc gatcttgcgc cgctcgtagc gccgcgactg cgatttgccc gcccagtcct
      901 gctccaggtg cttgagcagc gcgagcacca cctcgtcgtc gtagttgccg ttgagatcga
      961 gcccttcggg gagcgtcctc gtatacgcga cgtgcgcacg aagctgctcc agctcgtgca
     1021 gtccgcgtcc cgcgccgaag tagcgcagcc ccttgccggg cggcggctca cgcacgagcc
     1081 gcaccggcgc gttcggcgca gcgagatcga tccaatgcgt gcatccgtcg tcgggtcgcg
     1141 gactgatcac gaagaatccc gcaaaatagg cgacgagacg ggtagcgaga tgctgtgacg
     1201 agggcagcag gctgtcggtg gacgaggcgg ccagcatcag cgccttgagc agttcctgct
     1261 tcgagctgcc gctgccgctc gatccagggt aaaccgcgat cgcctcatcg tcgaagccgt
     1321 gttcctcggc gtgctgatag aggcgcgcga tttcggtcca gatgcgtggt tcggcggact
     1381 cgtagcgcaa cagcgaccag cgcaactgct ggcgcagcgt gcgcagcgcc cgcccgacga
     1441 agatcggaac cgtgctcttt cccgcggagc cgcccttccc gccctcgcgc acacaacgct
     1501 ggtattccgc gcccagttcc cgccagaaac cgtaagcgct gttccacagc cgttgctcgt
     1561 gggacttctt gtgacggggc gtagccagat attcgagaag cagggcgcgc tcgtgcgacc
     1621 gcgaagcgcc gtccaggagg tcgagattgc gaaaccggtg atcgagctta agctcgtcgt
     1681 tgcttcgcag cgcctggagc tctctcgtaa tctgctgcag ggcttcggcg ctgtcgtcag
     1741 ccggcaggct tgcgacgaac gcctgcagcc gcgcgctctc gctagccggc tgctcggctt
     1801 tcgcgcgccc gaaccagctg atcaagtcgt tccctcccca tccgcgctag acgccctcag
     1861 gcgcgattgt agccaaaaac tcctagcgcg cgcagggcga cccgtcgcct tgcgctgaga
     1921 tggccgcgcg ccgaccccgt gcgggatcga ccgttgcgac gatcgccatg ccaatcacga
     1981 agtaggttcc ggtcacgaga atcgcggtgc ggtgatcacc attcgtaatc cacgtgacgg
     2041 cgccatacgt gaccggcccg aggattgagg agagcttgac ggcgagcccc cacagcccga
     2101 agaactcggc gatacggtca ggcggactga gatagccgac gagcgcacgc cctgcagact
     2161 ggctcgaacc gaggcacagc ccggcgaggt tggctgcgat ccagaaaagc gtccgatcct
     2221 cggcagccca cgcgactacg acggtgacga gccagccgac gagcgtcgcc atgatcgtcg
     2281 gcacgtgccc gatgcggtcc tgcacgtggc cgaagaaaaa ggcgccgagc gccgccgtca
     2341 cattgacgac gagcaccaac accagcgtgt cacgggtcgt gaatcccatc gcctgctggg
     2401 cgtaaatcgc cgcgagtgcg acaaccgcct gtatgcccgc ctggtagaag acgaggcaga
     2461 tcagaaagcg gccgaaatcg ctgtaacgcc gagcatggtg cacggtccgg aaaaggcggg
     2521 aaaacgccgt cacgaccgcg tgagccggac gatcctcctg ccgaatcgcg cgctcgcgga
     2581 ggaagagaaa tgtcggcacg ctcgcgagcg cgaatatgct cgccgtaatg agcatggtca
     2641 ccggaacgaa ctcggtcgcg gcggcaccgc gttcctgcgc tgcgatcacg taggcaaggc
     2701 ttgctgcgag gctcacgagt ccgccgacat agccgagact ccagccccaa cccgatatgc
     2761 ggccgagacc gcgttcgcgc ccgagctcgg gcagaaatgc ggcgatcagg ttctctccgc
     2821 agccgaagaa gtaactcgac atcgccacca gcatgaaagc gagcacgacg tcgccgcggt
     2881 cggtaagcgc gagcgctgcc gtgaaaagga cgcaacccgc ggtggacagc gcgagcagcg
     2941 tcttcttgtt ggcacgcgcg tccgcatagg cgcccaacgc cggcgcgctc gccatga
//
LOCUS       DASURG010000011         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_820956, whole genome shotgun sequence.
ACCESSION   DASURG010000011 DASURG010000000
VERSION     DASURG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820956"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1152)
                     /locus_tag="VFY80_00205"
     CDS             complement(160..1152)
                     /locus_tag="VFY80_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006347.1"
                     /translation="MIHIGKAFRCPLRLLALVLGALCAAAPQAWAQNYPTRSIRMVLP
                     FPPGGPTDITGRVIAQKLSELLGQPVVPDNRPGAAGNIGLELAAKAPPDGYTIVLTAP
                     PIAISPSLYKKLNYDAQKELAPISLVAEIQNIVVTHNSVPVKNLKDLVALAQRNPGKL
                     TFSSSGAGSTNHLASELLKGRYKLDMIHVPFKGSSPALVALMSGQVDWGTMAVPGAIP
                     IINAGKVRGLAVLSEKRLPTLPNVPTAAESGVPDFVMSIWYGILAPAGTPREIITRLN
                     TEIHKALAAPDVKQRLAASGVDPLVSTPDQFATHIRAETVRYAKIIKDAGITAQ"
     gene            complement(1260..2315)
                     /locus_tag="VFY80_00210"
     CDS             complement(1260..2315)
                     /locus_tag="VFY80_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6006348.1"
                     /translation="MRANLFPNPLSALATLAIFYLAWQALPPFIDWALLDAVWRAPDA
                     NACRVEGAGACWAFIADKHRFILFGTYPYEAHWRPATATLLLIGLWGVSLARAAWKAW
                     LAAVWACGLLAIGGLMWGGVAGLSYVENERWGGLTLTLLLTTIGIGCAFPLAVVLAVA
                     RRSKLPVLRGLAVGYIELIRGVPLISLLFMASVMLPLFLPAGVSIDKLLRAQIALILF
                     AAAYLAEIVRGGLQAVPRSQYEAADALGFTYWSKMLLVILPQALRIAIPPLVNSFIGF
                     FKDTSLVLIIGLFDLLSTVKIALTDPAWSGFGVEAYLIASLVYFAFCFAMSRYSVRLE
                     REYARGQKAFWSLDLPR"
     gene            complement(2354..>2759)
                     /locus_tag="VFY80_00215"
     CDS             complement(2354..>2759)
                     /locus_tag="VFY80_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6006349.1"
                     /translation="EFATLAGGLILYTAAFIAEIVRAGVQSVPSGQSEAAYALGLSSS
                     RTLRHVVLPQALQAIVPPLTNQYLNLTKNSSLAVAIGYPDLVSIANTTINQTGQAIEG
                     IAIIMIVYLTISLSISALMNWYNARVALRGRG"
BASE COUNT          531 a          860 c          925 g          443 t
ORIGIN      
        1 gaagaactcg cggcatacgg cgttcgcgcc gcgggcctga agaccgacga agactgcgca
       61 catctcggaa aacatgtgcg cctcaccaac cgctagcagt tcatcgtaaa caacccgtca
      121 caccggcggc ctaagcgcgt gcagggtgtt acgtgcggcc tactgcgccg tgatgccggc
      181 gtccttgatg atcttcgcgt agcgcaccgt ctcggcgcgg atatgcgttg cgaattgatc
      241 cggtgtactg accagcggat cgaccccgga agcggctagg cgctgcttca catcgggcgc
      301 ggcgagcgcc ttgtgaattt cggtgttgag gcgggtgatg atctcacgcg gcgttccggc
      361 tggcgcaaga atgccgtacc agatcgacat gacgaaatcc gggacgccgg attcggcggc
      421 ggtcgggacg ttgggcaacg tcggcaaccg tttttcggag agcactgcca gcccccgcac
      481 cttgccggca ttgatgatcg ggatcgcacc cggcacggcc atcgtgcccc agtccacctg
      541 gccgctcatg agcgccacga gcgctgggct cgagcccttg aacggcacgt gaatcatgtc
      601 gagtttgtag cggcccttca gaagctcgct cgcgagatgg ttcgtgctgc ccgcgccgct
      661 cgagctgaag gtaagcttgc ccgggttgcg ctgggcgagc gccacgagat ccttgaggtt
      721 ctttaccgga accgaattgt gcgtgactac gatattctgg atttccgcga cgagtgaaat
      781 cggcgcgagc tccttctgcg catcgtaatt gagcttcttg tagagcgacg ggctgatcgc
      841 gatcgggggc gcggtaagca cgatggtata accgtcgggc ggtgccttgg cggcgagctc
      901 gagaccgatg ttgccggccg ctcccggccg gttgtccggc accacgggct gaccgaggag
      961 ttcggagagc ttctgcgcga tcacgcggcc cgtgatgtcc gtcgggccgc cgggcgggaa
     1021 cggcagcacc atgcggatcg accgggtggg gtagttctgt gcccacgcct ggggcgccgc
     1081 agcgcaaagg gcgcccagca cgagcgcgag caaccggagc ggacaacgaa acgccttgcc
     1141 gatgtgaatc atgctttctc cccaatcgag ttggataact gttgcgtctt ctgacgacgg
     1201 cggtgtgcgg gaacgtcgcg gaaacggcag ccgaaatgtt gcacgccgcc cgtgcgttgt
     1261 caacgcggaa ggtcgagcga ccagaacgct ttttgccctc gagcgtactc gcgctcgagg
     1321 cggacgctgt agcgcgacat cgcgaaacag aacgcgaagt acaccagtga agcgatgagg
     1381 tacgcctcca cgccgaatcc cgaccacgcc gggtcggtga gcgcgatttt taccgtggac
     1441 agcagatcga agagcccgat gatgagcacg agcgacgtat ccttgaagaa tccgatgaag
     1501 ctgttgacga gcggcgggat cgcgatgcgc agcgcctggg gtaggatgac gagcagcatc
     1561 ttgctccagt aagtgaagcc cagcgcatcg gccgcctcgt attgcgagcg cggaaccgcc
     1621 tgcaagccgc cgcgcacgat ttctgcgagg tacgcggcag cgaaaaggat cagcgcgatc
     1681 tgcgcgcgca gcagcttgtc gatcgacacg cccgcaggaa gaaagagcgg cagcatcacc
     1741 gaggccatga agagcagcga gatgagcggc acgccacgga tgagctcgat ataaccgacc
     1801 gcgagcccgc gcagcacggg cagtttcgag cggcgcgcga cggcgagcac gacggcgagc
     1861 gggaaggcgc agccgatgcc gatggtcgtg agcagcagcg tcagagtgag cccgccccag
     1921 cgttcgtttt cgacgtagct caggcccgct accccgcccc acatgagccc gccgatcgca
     1981 agaagcccgc acgcccacac ggcggcgagc caggctttcc atgcggcgcg cgcgaggctc
     2041 accccccaca gcccgatcag aagcagggtc gccgtcgcgg gccgccagtg cgcctcgtaa
     2101 ggatacgtcc cgaacaggat gaagcggtgc ttgtccgcga tgaacgccca gcacgcgcct
     2161 gcaccttcca cccggcacgc gttcgcatcg ggcgcgcgcc acaccgcgtc gagcagcgcc
     2221 cagtcaatga agggtggcag cgcctgccag gcgaggtaga aaatggcgag cgttgccagc
     2281 gcgctcagcg gattcggaaa cagattcgcg cgcatccagc ccggaaagcc cgcgagagac
     2341 cgccctttgc ccgtcacccc cgcccccgca gcgcgacccg cgcgttgtac cagttcatga
     2401 gcgccgaaat cgagaggctg atggtgagat agacgatcat gatgatcgcg ataccttcga
     2461 tcgcctggcc ggtctggttg atggtggtgt tggcgatgga aacgagatcg gggtagccga
     2521 tcgcgaccgc gagcgaactg ttcttcgtga gattgagata ctgattggtg agcggaggca
     2581 cgatcgcctg cagtgcctgc ggcagcacca catggcgcag cgttcggctg ctcgacagtc
     2641 ccagcgcata agcggcctcg gactggccgg agggtaccga ctgcacgccg gcgcgcacga
     2701 tttccgcgat gaaagcggcg gtgtacagga tgagcccgcc cgcgagcgtg gcgaattcc
//
LOCUS       DASURG010000012         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1229323, whole genome shotgun sequence.
ACCESSION   DASURG010000012 DASURG010000000
VERSION     DASURG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229323"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1312
                     /locus_tag="VFY80_00220"
     CDS             230..1312
                     /locus_tag="VFY80_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004867878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX6006350.1"
                     /translation="MRGDSTQQLEMLSPVTPDTLVPRGHPIREIRAIVDSALGELSPV
                     FDAMYAKVGRPSIPPEHLLKAMLLQAFYSIRSERQLCERLQYDLLFKWFVGLNILDPV
                     FDHSTFSKNRDRLLEHDVATHFFLAVRSEARRRHLLSSEHFTVDGTLLDAWASMKSVQ
                     PRDERGGPPPGGKNPTVDFHGQRRTNETHASTTDPEARLARKAAGQATKLAYAGHVLM
                     ENRSGLIVDIVLSQADGRAERAAALAMLDRQERPQRVTLGADKGYDTADFIAACRERG
                     VTPHVAQHTNRRRSRIDERVTRHTGYAVSQRVRKRVEEIFGWVKTIGGGRKLRYIGLA
                     RNQLWATLTGTAFNLVRMSRIERQPA"
     gene            1468..>2062
                     /locus_tag="VFY80_00225"
     CDS             1468..>2062
                     /locus_tag="VFY80_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006351.1"
                     /translation="MRANSPRGDNPHNIDDFLRAHGYNTPEARQRARAALEAAGLTNP
                     RKKNIAAYKLPDAERALSAALVRVCGDACVRLARSMRTERREPVITTGATCEVCGGSN
                     NRRATITCAALLKRKKVKRVLVVGGTAPQQHDLRSLLEGDGLTLEFVDGTNKSHTQRD
                     AIANMRRCDLVVIWGPTPLRHAVSNHYTNELVPDVRII"
BASE COUNT          394 a          713 c          653 g          302 t
ORIGIN      
        1 ctgcgatgag attcctcgcg gcgggtcgga gtgacgggag gtgggcgctg ttgattgaag
       61 tgcgtttgtg cagcgatgag attcctcgct gcggctcgga atgacggggg gtgggcgacc
      121 tcagtcctcc aacctctagc agggtgttga aatagtagcc gcgaaaggga tcgactcgcg
      181 atcccgggcg gcgtacgatg gcatcggcgg gacgcaagga ggcgagccga tgcgcggaga
      241 ttccacacaa caactggaga tgctcagccc ggtgacgccg gacacgcttg tgccgcgcgg
      301 gcatcctatc cgcgagatcc gcgccatcgt ggatagcgcg ctcggcgagc tctcacccgt
      361 gttcgatgcg atgtatgcga aggtggggcg cccgtcgatc ccgccggagc acctgctgaa
      421 ggcgatgctc ctccaggcct tctactccat ccgcagcgag cgccagctgt gcgaacgcct
      481 ccagtacgac ctgctcttca agtggtttgt gggcctcaac atcctcgatc cggtgtttga
      541 ccactccaca ttctcaaaga accgcgaccg cctgctggag cacgatgtgg cgacgcactt
      601 ctttctcgcc gtacgcagcg aagcacgccg ccggcacctg ctgtcgtccg agcacttcac
      661 tgtcgatggc acgctcctgg atgcctgggc gtcgatgaag agcgtccagc ccagagacga
      721 gcgcggcgga ccgccgccgg gcggcaagaa cccgaccgtc gacttccatg ggcagcggcg
      781 cacaaacgag acgcacgctt ctacgaccga tccggaggcg cggctggcgc gcaaggccgc
      841 cggccaggcg acgaagctcg cctacgcggg acatgtactc atggagaacc gcagcgggct
      901 gatcgtggat atcgtgctga gccaagcgga cgggcgggca gagcgggcgg cggcgctcgc
      961 catgcttgac cggcaggaac gcccccagcg cgtcacgctc ggcgccgaca agggatacga
     1021 caccgcggac ttcatcgcgg catgtcgcga acgcggcgtc acaccgcacg tcgcgcagca
     1081 cacgaaccga cggcgcagcc gcatcgatga gcgcgtgaca cgacacacgg gctacgcggt
     1141 aagccaacgc gtgcgcaagc gcgtggagga gatcttcggc tgggtcaaaa cgatcggcgg
     1201 tggacggaag ctccggtaca tcgggctcgc gcggaaccaa ctctgggcga cgctcaccgg
     1261 caccgccttc aacctcgtgc ggatgagtcg catcgagcga cagcccgcgt aggcgcacac
     1321 cccgcgcacc aactgactcg atgaggcgcc cggcaaggcg gaaaacggat gcaccccgtc
     1381 cgatgcaacg cctaaactgg ggcctacggt cgcttcttca acaccctgct aggtcctggg
     1441 gttccccaga agcgttatac tgtgatcatg cgagccaact cgccccgtgg cgacaaccct
     1501 cacaacatcg acgactttct gcgcgcccac ggctataaca cgcccgaagc acgccagcgc
     1561 gctcgcgctg ccctcgaagc cgccggcctc acgaaccccc gcaagaagaa catcgccgcc
     1621 tacaagctgc ccgacgccga acgcgcgcta tccgccgcgc tcgtccgcgt ctgcggcgat
     1681 gcctgcgtcc gcctcgcgcg atcgatgcgc accgagcgcc gcgagcctgt catcaccacc
     1741 ggcgccacgt gcgaagtttg cggcggctcg aacaaccgcc gcgcgaccat cacctgcgcg
     1801 gcgctcctca agcgcaagaa ggtaaagcgc gtgctcgtcg tcggcggcac ggcgccgcag
     1861 caacacgatc tccggtcgct gctcgaaggc gacggcctca cactcgagtt cgtggacggc
     1921 acgaacaagt cacacacgca gcgagacgcg atagcgaaca tgcgtcgctg cgacctcgtc
     1981 gtgatctggg gtccgacgcc gctgcgccac gccgtatcca accactacac gaacgagctc
     2041 gtcccggacg tccgcatcat ca
//
LOCUS       DASURG010000013         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_412436, whole genome shotgun sequence.
ACCESSION   DASURG010000013 DASURG010000000
VERSION     DASURG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412436"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFY80_00230"
     CDS             complement(<1..809)
                     /locus_tag="VFY80_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006352.1"
                     /translation="MKTAMSVLIVLVAPAFHAMAGAQAFPSRPVRLVVPYPPGGANDI
                     IARLLAPRLGATFGQNVIVDNRGGGNTIIGSEIVARSAANGYTILIVAAGHTINPSLY
                     PKLPYDTARDFAPIVLIGDGAYVLVAHPSTGVASTAELIALARKKPGQLAYASSSTGN
                     LTHLAAELFNSLAGVKMLHVPYKGGNPAMADLLAGRVAVFFSTVAVARAHLLSGKIRG
                     LGVTTAKRSLALPDMPTIAESGLPGYEVSGWYGLVAPVKTPKEPIARIHRAV"
     gene            complement(846..2075)
                     /locus_tag="VFY80_00235"
     CDS             complement(846..2075)
                     /locus_tag="VFY80_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006353.1"
                     /translation="MSSLSGARLVAIVCAAQVLAQIGAYFWPALLPTMIPLWGLTNSE
                     AGWITGIFYAAYLVSVPVLVTLTDRIDAKRVYLFGVACTVIAHVLFGLYAEGFWTALA
                     TRALAGMGWAGTYMTGLKLLADRVDSTLMARAVSGHAAGVGVSGALSFSCAGLLADAY
                     GWPAAFIIAGLSAALAWLFVWFSVPRRARTDKAATGAPLFDFRPVLRNRSAMAYSIGY
                     CIHTLEMNALRGWGVAFLGFVAASAGAGVVHPALSPTAVLTGLGLLGTLASVLGNEAA
                     IRLGRRRLIGFAMLASATVAAAIGFIGTLSYEMAVALLLLYGFIVWLDSSSLTAGSAG
                     TADPARRGATLAIHSMLGYAGGFVGPLMIGVILDLSGGMSRAGWRASFLAIGALMLAA
                     FVVFLAMRPRVLAGDRQ"
     gene            complement(2158..>2441)
                     /locus_tag="VFY80_00240"
     CDS             complement(2158..>2441)
                     /locus_tag="VFY80_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6006354.1"
                     /translation="ASVPFYGSQPAVADVPNIRAALLIHYAEHDERINKGWPAFEAAL
                     KANRVRYEAHFYPGTQHAFHNDTTPRYDEAAAKLAWQRTVDFFNTHLRG"
BASE COUNT          476 a          803 c          792 g          370 t
ORIGIN      
        1 accgctcgat ggatgcgtgc tatcggttcc ttcggcgtct tcaccggcgc cacgaggcca
       61 taccatccgc tcacttcgta gcccggcaat cccgattcgg cgatcgtcgg catgtcgggc
      121 agcgcaaggc tgcgctttgc agtcgtgacg cccagcccgc gaatttttcc ggagaggagg
      181 tgcgcacgcg ccaccgcgac cgtggagaaa aagaccgcca cacggcctgc gagcaggtcc
      241 gccatcgccg gatttccgcc cttgtacgga acgtgcagca tcttcactcc ggcgagcgaa
      301 ttgaaaagct ccgcagcaag atgggtgaga tttccagtac tcgaggacgc gtaggcgagc
      361 tgcccgggtt tcttgcgcgc gagggcgatg agctccgcgg tggacgccac gcccgtcgaa
      421 ggatgcgcca ccaatacgta cgccccgtct ccaatcaata cgatcggcgc gaagtcacgc
      481 gcggtgtcgt acgggagctt ggggtagagg ctgggattga tggtgtgccc ggccgcaacg
      541 atgagaatgg tgtagccgtt cgcggccgac cgcgcgacga tctccgatcc gatgatcgta
      601 ttaccgccgc cgcggttgtc cacgatcacg ttctgcccga atgtcgcgcc aaggcgcggt
      661 gcgagcagcc gcgcgatgat gtcgttggcc ccgcccggcg gataaggcac cacgagccgg
      721 accggacggc ttggaaacgc ctgggcgccc gccattgcgt ggaatgccgg cgcaacgagc
      781 acgatgagca cgctcatcgc agttttcatc gaagacatca tttcaatatt ccgcctgcat
      841 ggatttcatt gtcggtcgcc ggcaagcacg cgtggccgca tcgccaagaa tacaacgaag
      901 gccgccaaca tcaatgcacc gatcgcgaga aacgacgcgc gccagcccgc ccgcgacatt
      961 ccgcccgaaa gatcgaggat cacaccgatc atcagcgggc caacgaatcc acccgcataa
     1021 cccagcatcg agtggatcgc cagtgtggcg ccgcggcgcg cgggatcggc ggttccggcc
     1081 gaccccgcag tgagcgatga ggaatccagc cagacgatga agccgtagag aagcagcagc
     1141 gcgaccgcca tctcgtacga gagcgtgccg atgaagccga tcgcggcagc cacggtagca
     1201 gacgcgagca tcgcgaatcc gatgagacgc cgccggccga ggcggatcgc cgcttcgttg
     1261 ccgaggacgc ttgcgagcgt gccgagcagc cccagtcccg tcaacaccgc ggtcggcgag
     1321 agcgccggat ggacgacgcc ggcgccggcg ctcgccgcga cgaagcccag gaaggcgacg
     1381 ccccatccgc gcaatgcgtt catctcgagc gtgtgaatgc agtaaccgat ggaatacgcc
     1441 atggccgagc ggttgcggag cacgggccgg aaatcgaaga gcggagcgcc ggtcgcggcc
     1501 ttgtcggttc gcgcacgacg cgggacggaa aaccagacga agagccacgc gagcgcggcg
     1561 ctcaatccgg cgatgatgaa cgcggcaggc cagccgtagg cgtcagcgag gagcccggca
     1621 cacgagaacg aaagcgcgcc cgatacgccc actcccgccg catgccccga gaccgcgcgc
     1681 gccatgagcg tggaatcgac gcggtctgcc aggagcttga ggccggtcat gtaggtgccg
     1741 gcccagccca tgcccgccag cgcacgggtc gcaagcgcgg tccagaatcc ttcggcgtag
     1801 agcccgaaca ggacgtgcgc gatcaccgtg cacgcaacac cgaacagata cacccgcttg
     1861 gcatcgatgc gatcggtaag ggtcaccagt accggcaccg acacgagata cgccgcatag
     1921 aagatgccgg tgatccagcc cgcttcgctg tttgtgaggc cccacagcgg tatcatcgtc
     1981 ggaagcaggg ccggccagaa ataagcgccg atctgcgcca gcacctgcgc ggcgcagacg
     2041 atcgcgacca gccgtgctcc ggatagtgag ctcactcatc tccccttcgt caccccggcg
     2101 caaggcgggg tccagtcttg ctggattctg gcgtgcgccg gaatgacgcg cgctgtttca
     2161 gccgcgcaga tgcgtgttga agaaatccac cgtgcgctgc caggcgagct tcgccgccgc
     2221 ctcgtcatag cgcggcgtcg tatcgttgtg gaacgcgtgc tgtgtgcccg gatagaagtg
     2281 cgcttcgtac ctgaccctgt tcgctttcag cgccgcctcg aatgcgggcc accccttgtt
     2341 gatgcgctcg tcgtgctccg cataatggat gaggagcgcc gccctgatat tcggcacatc
     2401 ggcgaccgca ggctggctgc cgtagaaggg taccgaggcc g
//
LOCUS       DASURG010000014         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_923658, whole genome shotgun sequence.
ACCESSION   DASURG010000014 DASURG010000000
VERSION     DASURG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923658"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFY80_00245"
     CDS             <1..495
                     /locus_tag="VFY80_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX6006355.1"
                     /translation="ASDRMGRRQTGETMRVLGLIALLVAAAAQAQIYECVDANGNKRF
                     TNIKSEAKGCKQLDVGPINTVPAYKPAPKGAPAPANFPKVDATTQRERDTDRRRILEQ
                     ELATEQKQLAAAQKALAEQQAIRQGDERNYQRVLDRLEPYQKRVKQHEENIESLKREL
                     ANLR"
     gene            560..1636
                     /gene="glnL"
                     /locus_tag="VFY80_00250"
     CDS             560..1636
                     /gene="glnL"
                     /locus_tag="VFY80_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX6006356.1"
                     /translation="MTMETFSGLDLLATAVIVLDEKLNVQYLNAAAENLLEVSNRSAA
                     GVPLAKVFREDGKLLAAIGYACAHNCSYTEHDLSLAVDNRKLDLACTVTPVEVAPAGA
                     FLLELRHVEQQLKIAREERLLDQNRANRELIRNLAHEIKNPLGGIRGAAQLLDRELER
                     PDLHEYTQVIMKEADRLQILMDRLLAPHRPPKPVLLNVHEVLERVRSVILAEYIEGLV
                     IHRDYDSSLPPLKGDREQLIQAFLNIVRNAAQALNGKGQIWLRTRIARQVTLVRKRHR
                     HAIEVTICDNGPGIPEAIRDRILYPLVSGRDNGTGLGLTIAQTFITQHHGTIAYDSAP
                     GNTCFTIMLPVDNGEDAVLSGADQ"
     gene            1633..3036
                     /gene="ntrC"
                     /locus_tag="VFY80_00255"
     CDS             1633..3036
                     /gene="ntrC"
                     /locus_tag="VFY80_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX6006357.1"
                     /translation="MKPVWIIDDDRSIRWVFEKALMRESIAFRTFASVREALDALETD
                     TPQVLVSDIRMPGESGLQLLQSAKARYPNLPVIIMTAYSDLESAVTAFQGGAYEYLPK
                     PFDVDHAVELIRRAMQESARKNGAVEEVAAAPEILGQAPAMQELFRAIGRLSQSHATV
                     LITGESGTGKELVARALWRHSPRADKQFIAINTAAIPKELLESELFGHERGAFTGAQA
                     MRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGHFYRVGGHQPIKANVRVIAAT
                     HQDLDMRVKQGLFREDLFHRLNVIRLRLPPLRERREDIPLLARYFLHKCARELGVEAK
                     RLSEATVRYLGAQDFPGNVRQLENLCHWLTVMAPSATIEIKDLPPEIRGDTPPSAEGW
                     IPLLEREVEALLSNGGIAIMEELTRKFEKALIARALAHTGGRRIEASQLLGLGRNTLT
                     RKIQELGLDSGKERNSV"
     gene            complement(3061..>3548)
                     /locus_tag="VFY80_00260"
     CDS             complement(3061..>3548)
                     /locus_tag="VFY80_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX6006358.1"
                     /translation="VCGMRAICIYVPNGEHVESDKYRYKLRWLSAVTAWLKGELGRHA
                     RLAVLGDFNIAPEERDVHDPKVWEGQVLFSALEREAFRDIVALGLADAFRLFDQPDQS
                     FTWWDYRLNAFRRSMGLRIDHILLSRELAARCTACSIDTGPRAAERPSDHAPVWADLD
                     L"
BASE COUNT          695 a         1155 c         1121 g          577 t
ORIGIN      
        1 gcaagcgacc gaatggggcg ccggcaaacc ggggaaacca tgcgagttct gggattgatc
       61 gccttacttg ttgcggccgc cgctcaggcg cagatctacg aatgcgtcga tgcgaatggc
      121 aacaagcgct tcaccaatat caagtccgag gcgaagggct gcaagcagct cgacgtcggg
      181 ccgatcaata cggtgccggc ctacaagccg gctcccaagg gtgcacccgc gcccgccaac
      241 tttcccaagg ttgacgcgac cacgcagcgc gagcgcgata cggatcgccg ccgcattctc
      301 gaacaggagc ttgcgaccga gcagaagcag ctcgctgcag cccagaaggc gctcgccgag
      361 cagcaggcga tacgtcaggg cgatgagcgc aattatcagc gcgtgctcga ccggctcgag
      421 ccataccaga aaagggtgaa gcagcacgag gagaacatcg aaagcctcaa gagagagctg
      481 gccaatctcc ggtaacgggc gttacgagtg gccgtatcgt ggcgcgcttc ttgctcccgc
      541 agtccaccgg tgcaaaggca tgacaatgga aacgttctcc ggactggact tgcttgccac
      601 tgcggtcatc gtgctcgatg aaaagctcaa cgttcaatat ctcaacgctg cggcggagaa
      661 ccttctcgag gtgagcaacc gcagtgccgc gggagttccg ctggccaagg tcttcaggga
      721 ggatggcaag cttctcgcag ccattgggta tgcctgcgcg cacaactgca gctacacgga
      781 gcacgacctc tctctcgctg tcgacaatcg caagctcgac ctcgcgtgta cggtcacgcc
      841 ggtggaagtc gcgccggccg gcgccttcct gctcgagctg cgccacgtgg agcagcagtt
      901 gaagatcgcg cgcgaggagc ggctgctcga ccagaaccgc gccaaccgcg aactcatccg
      961 caacctcgcg cacgagatca agaatccgct ggggggtata cggggtgctg cgcagctcct
     1021 cgaccgcgag ctcgagcgtc ccgacctgca cgagtacacg caggtcatca tgaaggaagc
     1081 ggatcggctc cagattctca tggaccgcct gctcgccccg caccgcccgc ccaagccggt
     1141 gctactcaac gtccacgagg tcctcgaacg cgtccgcagc gtgattttgg cggaatacat
     1201 cgagggactc gtgatccacc gcgactacga ttcgagcctc ccgccgctca agggcgatcg
     1261 cgagcagctc attcaggcgt tcctcaacat cgtgcgcaat gccgctcagg cgctgaacgg
     1321 caaagggcaa atctggctgc gaacgcgcat cgcgcgacag gttacgctcg tgcgcaagcg
     1381 ccaccgccat gctatcgaag tgaccatctg cgacaacggc cccggcatac ctgaagcgat
     1441 ccgagaccgc atcctgtatc cgctcgtgtc ggggcgcgat aacggcaccg ggctcgggct
     1501 cacgatcgcc cagactttca tcacgcagca tcacggcacg atcgcctacg acagcgcgcc
     1561 cggcaacacc tgtttcacca tcatgctgcc ggtggacaac ggcgaggacg ccgtgctttc
     1621 cggggcggat caatgaagcc cgtctggatc atcgacgacg accgctcgat acgctgggtc
     1681 ttcgagaagg cgctcatgcg cgagagcatc gcgtttcgca ccttcgcgtc cgtgcgcgag
     1741 gcgctcgatg cgctcgagac cgacaccccg caggtgctcg taagcgacat tcgcatgccg
     1801 ggtgaatcgg gcttgcagct ccttcagtcg gccaaggccc gctatcccaa cctgccggtg
     1861 atcatcatga cggcgtactc ggacctcgag agcgccgtca cggctttcca gggcggcgcg
     1921 tacgaatatc tgccgaagcc gttcgatgtc gaccatgccg tcgagctcat ccgacgcgca
     1981 atgcaggaga gcgcgcgcaa gaacggcgcg gtggaggagg ttgccgccgc gccggagatt
     2041 ctggggcagg cgccggccat gcaggagctc ttccgcgcca tcggccggct gtcgcaatcg
     2101 cacgcgacgg tcctgatcac cggagagtcc ggcaccggca aggagctcgt cgcacgtgcg
     2161 ctctggcgcc acagcccgcg cgcggacaag cagttcatcg cgatcaacac cgcagcgata
     2221 ccgaaggagc tgctcgaatc cgaactcttc ggacacgagc gcggcgcatt taccggggct
     2281 caggccatgc gtcgcggccg cttcgagcag gccgagggcg gtacgctctt tctcgacgag
     2341 atcggcgaca tgccggccga gctgcagacg cggctcctgc gcgtgctgtc ggacggtcat
     2401 ttctaccgtg tcggaggcca ccagccgatc aaggcgaacg tgcgcgtgat cgccgcgacg
     2461 caccaggacc tcgacatgcg cgtgaagcag gggcttttcc gcgaagatct ttttcatcgg
     2521 ctcaatgtca tccggctgcg gctgccgccg ttgcgggagc gccgcgagga tatcccgctc
     2581 ctcgcgcgct atttcctgca caaatgcgcg cgcgagctgg gcgtcgaggc aaagcgcttg
     2641 tcggaggcga ccgtccgcta ccttggcgcg caggattttc ccggcaacgt tcggcagctc
     2701 gaaaacctct gccattggtt gacggtcatg gcgccgtccg cgaccatcga aatcaaggat
     2761 ctgccgccgg aaatccgcgg ggatacgccg ccgtcggcgg agggctggat cccgctcctt
     2821 gagcgcgaag tggaggcgct gctcagcaac ggcggcatcg cgatcatgga agaactcacc
     2881 cgcaagttcg aaaaggccct gatcgcgaga gcgctcgctc acactggcgg gcggcgcatc
     2941 gaggcttcgc aattgctcgg cctcggtcgc aacaccttga cgcgcaaaat ccaggagctg
     3001 ggtctcgaca gcggcaagga gcgcaacagc gtatagggtc gctcgataac gccttcgaag
     3061 ctagaggtcg agatcggccc atacgggcgc gtgatccgag ggtcgctctg ccgcgcgcgg
     3121 tcccgtgtcg atcgagcacg cagtgcagcg tgccgcaagc tcccgggaga gcaggatgtg
     3181 atcgatgcgc aggcccatgc tgcggcggaa ggcgttcagg cggtaatccc accaggtgaa
     3241 tgactgatct ggctggtcga agaggcgaaa agcatcggca agccccagtg caacgatgtc
     3301 gcggaatgcc tcgcgttcaa gcgcgctgaa aagcacctgg ccttcccaca ccttggggtc
     3361 gtgcacgtcc cgttcctcgg gcgcgatgtt gaagtccccc agcacggcga gccgcgcgtg
     3421 gcgcccgagt tcccctttga gccaggcagt caccgccgaa agccagcgta acttgtaacg
     3481 atacttgtcg gactcgacgt gctcgccgtt cggcacgtaa atgcagattg cgcgcatgcc
     3541 gcagacgg
//
LOCUS       DASURG010000015         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_413008, whole genome shotgun sequence.
ACCESSION   DASURG010000015 DASURG010000000
VERSION     DASURG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413008"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFY80_00265"
     CDS             complement(<1..972)
                     /locus_tag="VFY80_00265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6006359.1"
                     /translation="MNTPLPLSEPRALGFAPERLKRIETALRIEIEARTLPGAVLVIA
                     REGKIAYFETFGHLDPVARIAMPKDALFSIASMTKPIVAVGALMLCEEGRLMVNEPVA
                     RYVPQLAQMKVAAKCDGSETVPPARAMTIEDLMRHTAGLSYGRGATPLHARYPVSSDS
                     ASSKWTGREFLEALAQLPLHYQPGTSWEYGLGLDVLGLAIEAITGTPLARYLDERLFR
                     PLGMHDTSFTVPPGKVARYAKALTHDPVTREPQTLRDGTRPHKFDCGGGCAVSTAADY
                     LRFAQMLLDGGTLEGTRVLGRKMVEYMTSDHITPEIDTSRLREYPNIN"
     gene            1134..2435
                     /locus_tag="VFY80_00270"
     CDS             1134..2435
                     /locus_tag="VFY80_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX6006360.1"
                     /translation="MLKALTAIAVVSFVSLAACADTARLPFSAGVGANPTLPPPNRTL
                     IPTVEIAPAKGWPDGAKPTATAGMIVNAFATGLDHPRWLYVLPNGDVLVAESDAPPRE
                     SGGGMKDRLMKFFMRKAGSGFPSANRITLLRDADGDGVAEVRHAFLENLNSPFGMALV
                     GSDLYVANCDAVLRFPYKEGATRIDTPGVKVTDLPGAPNHHWTKNLLPSRDGTRLYIT
                     VGSNSNAAEKGMEIEEGRAAIWELDLKSGHKRVYASGLRNPNGMAWEPATGALWTVVN
                     ERDELGSDLVPDYLTSVREGAFYGWPYSYFGQHVDRRVEPQRPDLVAKALKPDYALGP
                     HVAPLGLVHSRGNALTAQFAEGMFVGQHGSWNRRPKSGYNVVFVPFKDGRPHGEPIEV
                     LTGFLSAAGEAYGRPVGVAIDRRGALLVADDVGNAVWRVSR"
BASE COUNT          439 a          802 c          829 g          463 t
ORIGIN      
        1 attgatgttg ggatattcgc gcagccggct cgtgtcgatt tcgggcgtga tgtggtcgga
       61 ggtcatgtat tcgaccatct tgcggccgag aacgcgcgta ccctcgagcg ttccgccgtc
      121 gagcagcatc tgcgcgaaac gcaaataatc ggccgcggtg gagaccgcac agccgccgcc
      181 gcagtcgaac ttgtgcggac gggtaccgtc gcgcagtgtt tgcggttcac gcgtcacggg
      241 gtcgtgtgtc agcgctttgg catagcgcgc cactttcccg ggcgggacgg tgaacgacgt
      301 atcgtgcatg ccgagcggcc tgaacaggcg ctcgtcgaga tagcgcgcaa gcggcgttcc
      361 cgtgatcgct tcgattgcaa gccccaggac gtcgaggccg aggccgtact cccacgacgt
      421 gccgggctgg tagtgcagcg gcagttgagc gagcgcttcc agaaactcgc gcccggtcca
      481 cttcgaagac gcgctatcgg atgaaaccgg atagcgcgca tgcaacggcg tcgcgccgcg
      541 accgtagctc aggcccgcgg tatgacgcat cagatcttcg atggtcatgg cgcgcgccgg
      601 cgggacggtc tcgctgccgt cgcacttcgc ggccactttc atctgtgcga gctgcggcac
      661 gtaccgggcc accggctcgt tcaccatgag gcgcccttct tcgcagagca tcagcgcgcc
      721 gaccgcgacg atgggcttcg tcatggacgc gatgctgaat agcgcgtcct tcggcatcgc
      781 tatccgtgcc accggatcga gatggccgaa agtctcgaaa taagcgatct tcccctcgcg
      841 cgcgatgaca agcaccgcgc cgggaagcgt ccgcgcttca atctcgatcc tgagcgcggt
      901 ttcgatgcgc ttcaggcgct ccggcgcaaa accgagcgcg cgcggttcgg agagtggcag
      961 cggtgtattc attgatttgt gcgcagcgcg gcgaaggcgt tattgtgctg cagcaagctt
     1021 aacaacaaga cgcgcgtgcg ggaactccat cccgcgcggc gtgccagagc gtacgcagtg
     1081 tcctattgct ttctgtcgtc cgttgccgcc cgcgtgcatt ttgctccagc cccatgctca
     1141 aagcactgac ggccatcgcc gtcgtttctt tcgtttccct cgcagcgtgc gctgataccg
     1201 ccaggctgcc tttctctgcg ggtgtcggcg cgaatcctac attgccgccg ccgaaccgga
     1261 ccctcattcc cactgtcgag atcgcgcctg ccaagggttg gcccgacggc gcgaagccga
     1321 ctgcaactgc cggcatgatc gtgaacgcgt ttgcgaccgg gctcgatcat cctcggtggc
     1381 tctacgtgct gccgaacggc gatgtgctcg tcgctgagag cgatgcgccg cccagggaaa
     1441 gtgggggcgg catgaaggac cggctgatga agttcttcat gcgcaaggct ggatccggtt
     1501 tcccgagcgc taaccgcatt accctcctgc gcgatgccga cggcgacggc gttgcggaag
     1561 tccgccacgc attcctcgag aacctgaatt cgcctttcgg catggcgctc gtcggatccg
     1621 atctctatgt cgcaaactgc gacgccgtgc tgcgctttcc ctacaaggag ggcgcgaccc
     1681 gcatcgacac gccgggcgtc aaagtgaccg atctgccggg cgcacccaat catcactgga
     1741 ccaaaaacct cctgccgagc cgcgacggca cgcgtctcta catcaccgtc ggctcgaaca
     1801 gcaacgcagc ggagaaaggc atggagatcg aggaaggccg tgcggccatc tgggagctcg
     1861 atctcaagag cgggcacaag cgcgtgtacg ccagcggact gcgcaatccc aacggcatgg
     1921 cgtgggagcc cgcgaccggt gcactgtgga cggtcgtgaa cgagcgcgac gaactcggga
     1981 gcgatctcgt tcccgattat ctgacctcgg tccgggaggg tgcgttctac ggctggccgt
     2041 acagctattt cggccagcac gtcgatcggc gcgtcgagcc gcagcggccc gatctcgtcg
     2101 caaaggcgct gaagcccgac tacgcgctcg gcccacacgt cgcgccgctc gggctcgtac
     2161 attcgagagg caatgcgctg acagcgcagt tcgccgaagg catgttcgtc gggcagcatg
     2221 ggtcgtggaa tcgccggccg aagagcggct acaacgtggt attcgtgccg ttcaaggatg
     2281 gcaggccgca cggcgagccg atcgaagtgc tgacaggatt cctgagcgcg gcgggcgaag
     2341 cctatggaag accggtgggt gttgcgatcg acaggcgcgg cgcattgctc gtcgcggatg
     2401 atgtcggcaa tgccgtctgg cgcgtcagcc ggtaagccgt tgccgcgcac ggcgagatgc
     2461 agagtgcata tcctgggcac cggcttccgc cggtgagacg attttcgctg cgtgatcggc
     2521 ttgcaccggt cag
//
LOCUS       DASURG010000016         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_617936, whole genome shotgun sequence.
ACCESSION   DASURG010000016 DASURG010000000
VERSION     DASURG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617936"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFY80_00275"
     CDS             <1..696
                     /locus_tag="VFY80_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX6006361.1"
                     /translation="GTQGAGGNQVEVAYNRTVDKAAGERAVTHEVPIVYTRGITDALD
                     LYIGVVGQRLEPPPPASSERGWGNTALGVKWRFYENDAAKLSLALKPEVRLPVAERRE
                     KRGLGTARATYAVGFLATQETGFGAIHANLAMERIDYADPALDAAERRTRYRASIAPV
                     WDVTERWKLALDAGWITQPDPAERRYLAYGEIGAIYAPMKDLEFALGVIRNFRDGAVR
                     TTQATIGLTWRFR"
     gene            741..1349
                     /locus_tag="VFY80_00280"
     CDS             741..1349
                     /locus_tag="VFY80_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006362.1"
                     /translation="MRVFAFLEYVFVIIGAIGMAAARYFYLPKGFHLGLFMVGAGFAL
                     GGLESLATRRMSFRFATHAGEGYNGAPAVIWGLMAFAIGSALIAAAYLMEEGLWRTTV
                     SSVMRRPGLAIAAAGLLAVGAGALLMFYRGRRGAWRTLFIRIPKALAGLVLIVGGVAA
                     VGLGIVEWIDHPAYARITQRGTEYLDLYGIRSTWRDFTRRFF"
     gene            complement(1370..>2029)
                     /locus_tag="VFY80_00285"
     CDS             complement(1370..>2029)
                     /locus_tag="VFY80_00285"
                     /inference="COORDINATES: protein motif:HMM:NF004779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX6006363.1"
                     /translation="RSAADLEAARNKIADAHKIGVQTYALDLSSAENAIKLARECGPV
                     DILVNNAGAIPQGNLTDLDDKTWRAAWDLKVFGFINLTREVYRDMCARKRGVIINVIG
                     TAGARPTAGYLAGSMANSALMTMSCALGADSTRFGVRVMGVNPTATATDRGVARWRNE
                     AAKALGDPERWPEMTKSFPFGRPATVEEVANVVVFLASDRASYMSGSVVNVDGGAVWR
                     K"
BASE COUNT          323 a          659 c          671 g          376 t
ORIGIN      
        1 ggtacgcagg gtgcgggcgg caaccaggtt gaggtcgcct acaaccggac ggtggacaag
       61 gcagccggcg aacgggcggt cacccacgag gtgccgatcg tttatacgcg tggcatcacc
      121 gacgcgttgg acctctacat cggcgtcgtc gggcagagac tcgagccgcc gcccccagca
      181 tcctcggagc ggggatgggg caataccgcg ctcggcgtga aatggcgctt ctacgaaaac
      241 gacgcagcca aactgagtct cgcgctcaag cctgaagtgc gcctcccggt ggcggagcgt
      301 cgcgagaaac gcggtctcgg caccgcacgc gcgacctacg ctgtcgggtt cctcgccaca
      361 caagagacgg gctttggagc gatccacgcc aaccttgcga tggagcgaat cgactacgcc
      421 gatcccgcgc tcgatgcggc ggagcgccgg acgcgctatc gggcgtcgat cgcgccggta
      481 tgggatgtga cggagcgctg gaaacttgcg ctcgacgccg ggtggatcac ccagcccgat
      541 cccgctgagc gccgctatct tgcttacggg gagatcggcg cgatctatgc gccgatgaag
      601 gatctcgagt tcgcgctcgg cgtcatccgc aattttcgtg atggcgccgt gcgcaccaca
      661 caggcgacca tcgggttgac gtggcgcttt cgctgacgat tcggctgcgc gcgcaagtgc
      721 gcttcggcta aactcgcgcc atgcgggtct tcgcgttcct ggaatatgtg ttcgtcatca
      781 tcggcgcgat cggaatggcg gcggcgcgtt acttctatct gcccaagggc tttcatctgg
      841 gactcttcat ggtcggcgcc ggtttcgcgc tcggcgggct ggaatcgctt gcgacgcgaa
      901 ggatgagttt ccggttcgcg acgcacgctg gcgaaggcta caacggcgcg ccggccgtca
      961 tctgggggct gatggccttt gcgatcggct cggcgctgat cgctgcggcc tacctcatgg
     1021 aggaagggct ctggcgcacc acggtgagca gtgtgatgcg ccgtccggga ctcgcgatcg
     1081 ccgccgcagg actgctcgcc gtcggcgcgg gcgcgctcct catgttctat cgcggcaggc
     1141 gcggcgcctg gcgcacgctt ttcatccgta taccgaaggc gcttgcgggg ctcgtgttga
     1201 ttgtcggcgg ggtggccgcc gtcgggctcg gcatcgtcga atggatcgat catccggcct
     1261 acgcgcgcat cacgcagcgc ggcaccgagt acctcgatct ctacggcatc cgcagtacgt
     1321 ggcgcgactt cacgcgccga ttcttctgac aaaccgtccc tgcctcggtt tacttacgcc
     1381 atactgcgcc gccgtctacg ttcaccacgc tgccgctcat gtaggaggcg cggtcagagg
     1441 cgagaaacac cacgacgttc gcgacttcct cgaccgtggc cgggcggccg aagggaaagc
     1501 tcttcgtcat ttcaggccag cgctccggat ctccgagcgc cttggcggcc tcgttgcgcc
     1561 agcgggcaac cccgcggtcg gtcgccgtcg cggtcggatt gacccccatt acacggacgc
     1621 caaaccgcgt gctgtccgcg ccaagcgcgc acgacatcgt catcaaggcg gaattggcca
     1681 tgctacccgc gagataaccg gccgtcggac gcgcgcccgc agtgccgatc acgttgatga
     1741 tcaccccgcg cttgcgggcg cacatgtcgc gatagacttc gcgcgtcagg ttgatgaaac
     1801 cgaagacctt gagatcccat gcggcgcgcc acgtcttgtc gtcgagatcg gtgaggttgc
     1861 cctgtggaat cgcgcccgcg ttgttgacga gaatatcgac cggcccgcac tcgcgcgcga
     1921 gcttgattgc gttctccgcc gacgatagat cgagcgcgta ggtctggact ccgatcttgt
     1981 gtgcatccgc gatcttgttg cgggcggctt cgagatccgc cgcgctgcg
//
LOCUS       DASURG010000017         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_719633, whole genome shotgun sequence.
ACCESSION   DASURG010000017 DASURG010000000
VERSION     DASURG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719633"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..445)
                     /locus_tag="VFY80_00290"
     CDS             complement(65..445)
                     /locus_tag="VFY80_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6006364.1"
                     /translation="MNEALYEKGMATRRKVLGDAHIARRQASDDKYTKQQNELVTEVA
                     WGAIWSRPQLPLKIRSLVTLGMLAALNRPDETKGHIGGALNNGATPEEIVEVFVQAAA
                     YCGFPASNGCVRLAVEVFRERGLI"
     gene            620..1642
                     /locus_tag="VFY80_00295"
     CDS             620..1642
                     /locus_tag="VFY80_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX6006365.1"
                     /translation="MADARATAPGYPPDALLRSTPETVEWGYIRADRAPVLRIKSGQT
                     VRIDTVSHQGLNTKDDPVAYFGAAGIAPVDVLEDAKAIYRTMKRAEGAGPHIVTGPIY
                     VEEAEPGDMLEVRMLDVAFRVPYGVNSTGPGWGAAPDLLEKPAAKMIRLDLARKVALF
                     APNVEVPLAPFMGIVAVAPPPQTPRVSTKPPGPFGGNMDFRHLTAGSILYLPVFNEGA
                     LFYTGDGHAVQGDGEVDGTAIEISLAATVQLIVHKGAGRDMTWPRAEDDRHHYVMGMD
                     ADLGKALHYAIRETVLFLERRAKLERAEAYALCSLAVDFRIGEAVNNVLMVYGAIPKN
                     IFKQAI"
     gene            1654..2115
                     /locus_tag="VFY80_00300"
     CDS             1654..2115
                     /locus_tag="VFY80_00300"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6006366.1"
                     /translation="MAGGKSRARSKSSDGNSAKAAQDSPVMQGGMARALGIRLTSLTK
                     KKVMAEMDVQPMHLNRSGRVSGGAIMAFADIVGATGAVFNLPPGHRSGTIESKTNFFA
                     AAKGPVLKAVSVPLHFGRTTTVWQTTVKNGDGKVAAIVTQTQIVIPAKSPD"
     gene            2211..>2683
                     /locus_tag="VFY80_00305"
     CDS             2211..>2683
                     /locus_tag="VFY80_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6006367.1"
                     /translation="MKVWWQSSTPIHRLHDYRTTLGEHLHAVKRADTEVHINGVDDGS
                     MDLHYNAVVAINSYGPGGVLNKIMQAADQGYDAVAIGCFLDPAMQEAREVVPIPVFGL
                     GETSMLTACMYGYKFSGVAFHAKQSQYYDRKAYEYGLASRHIPFGDLGIDFNEVQ"
BASE COUNT          524 a          820 c          845 g          494 t
ORIGIN      
        1 atcctcgtag atcaccatcc cgccgtttcg cgccacccgg cctgatgcgt gaaggggaaa
       61 cttatcagat caacccgcgc tccctgaaca cttccaccgc aagccgcacg cagccgttcg
      121 aggccgggaa tccgcagtaa gccgcggcct gcacgaacac ttccacgatt tcctcgggcg
      181 tcgcgccgtt gttgagcgca ccgccgatgt gtcccttcgt ttcgtcggga cggttgagcg
      241 ccgccagcat gccgagcgtc acgaggcttc tgatcttgag cgggagctgc ggccgcgacc
      301 agatcgcgcc ccatgcgact tcggtcacca gctcgttctg ctgtttggtg tatttgtcgt
      361 cgctcgcctg acgtctcgcg atatgcgcat cgccgagcac tttccgccgc gttgccatgc
      421 ctttctcgta gagcgcttcg ttcatgtgac ctccttcgat tgaaccgcag cgcgattgtc
      481 gtcgatttga cgggaggtcg acaggggccg ccgtcgcgaa aacggtgcta atatcgagcc
      541 gtaaagcacc gaagaacctt cccatggctt cagtcttaag gcgcgcccgc gcgctttcgc
      601 ttcgaaacgg aggaggatga tggcggatgc gcgcgccacg gctcccggtt accctcctga
      661 tgcgctcttg cgctcgacac cggaaaccgt ggaatggggg tacatcagag ccgaccgggc
      721 gccggtgctg cgcatcaaat ccggacagac cgtgcgcatc gacacggttt cgcatcaggg
      781 cctcaatacg aaggacgatc cggttgccta tttcggcgcg gccggcatcg cgccggtgga
      841 cgtcctcgag gatgcgaagg cgatctaccg cacgatgaag cgcgcggagg gtgctggccc
      901 gcacatcgtc acgggaccca tatacgtcga agaggccgag cccggcgaca tgctcgaagt
      961 tcgcatgctc gatgtcgcgt ttcgcgtgcc gtatggcgtg aattccacgg gaccgggatg
     1021 gggcgccgcg cccgatctgc tggagaagcc ggccgccaaa atgatcaggc tcgatctcgc
     1081 gcgcaaggtc gcgctcttcg cgcccaacgt ggaagtcccg ctcgcgccgt tcatgggcat
     1141 cgtcgcggtc gcaccgccgc cgcagacgcc acgggtcagt accaagccgc ccggcccgtt
     1201 cggcggcaac atggatttca ggcatttgac ggcgggctcg atcctttatc tccccgtatt
     1261 caacgagggc gcattgttct atacgggaga cggacatgcg gtgcagggtg atggcgaagt
     1321 ggatggcacg gcgatcgaaa tttcgcttgc cgcgacggta cagctcatcg tccacaaagg
     1381 cgcaggccgc gacatgacat ggccgcgtgc cgaagacgat cggcatcatt acgtgatggg
     1441 gatggacgcc gacctcggca aggccctgca ttacgcgatc cgcgaaaccg tcctgttcct
     1501 agaacgtcgc gcaaaactcg aacgcgccga ggcgtatgcg ttgtgcagcc ttgcggtgga
     1561 cttccggatc ggcgaggcgg tcaacaacgt gctgatggtc tacggggcga tcccgaaaaa
     1621 catattcaaa caggcgatct aggagcgtac ggaatggcgg gaggcaagtc gcgggcaaga
     1681 agcaaatcga gcgatggtaa tagcgcaaag gcggcccagg attcaccggt gatgcagggc
     1741 ggcatggcgc gtgcgctggg gatccgcctt acttcgctca cgaagaagaa agtgatggct
     1801 gaaatggacg tgcagcccat gcacctcaac cggtccgggc gggtgagcgg aggcgcgatc
     1861 atggctttcg ccgatatcgt cggcgcgacc ggcgccgtgt tcaacctgcc gccgggacac
     1921 cgcagcggca ccatcgaatc gaaaaccaac ttctttgcgg cggcaaaagg gccggtgctg
     1981 aaggcggttt ccgttccgct gcatttcggc cgcacgacga cagtgtggca gacgacggtc
     2041 aagaacggtg acggcaaggt cgccgccatc gtgactcaga cgcagatcgt cattccggcg
     2101 aagtctcccg actgaggggt cacgcagacg agtcttgatc caggtcaagg gatgcagcag
     2161 cgcgtgcctt attctgcccg actggggtat atcgcccgga aggagatcct atgaaagtct
     2221 ggtggcagag cagcacgccc attcatcgcc tgcacgacta tcgcacgacg ctaggcgagc
     2281 atctccatgc agtgaagcgt gccgatacag aggtccacat caacggcgtg gacgacggct
     2341 cgatggattt gcattacaac gcggtggtcg cgatcaacag ctacggaccg ggcggcgttc
     2401 tcaacaagat catgcaagcc gcagatcagg gttacgatgc ggtcgcgatc ggctgttttc
     2461 tcgatcctgc tatgcaggaa gcgcgggagg tcgtgccgat tccggtcttc ggcctgggcg
     2521 agaccagcat gctcacggcc tgcatgtacg gctacaagtt ctccggcgtc gcctttcacg
     2581 ccaagcagtc gcagtactac gaccgcaagg catacgagta cggcctcgcg tcgcgccata
     2641 tccctttcgg tgatctcggg atcgacttca acgaagtgca gaa
//
LOCUS       DASURG010000018         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_107814, whole genome shotgun sequence.
ACCESSION   DASURG010000018 DASURG010000000
VERSION     DASURG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107814"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFY80_00310"
     CDS             <1..994
                     /locus_tag="VFY80_00310"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HEX6006368.1"
                     /translation="FLLTPAAHAAEPSLIECDRATACELQIYIENDSAGGGTDRYYTN
                     GIKVGGGVHANRLIERLFQRPAEQVLRQFSEKPGDVHVGLFIGQHMYTPKRITVAEPQ
                     PFDRPWAAWLYVGGVAQSVAGNRLQTAEFDLGMIGPAALGKEMQTAWHELVGADRPVG
                     WHNQLKNEPGLMLTYLEKWRFGHPTGMELVPHFGGSVGNVMTLARVGGIVRAGKNMSG
                     FGPDTIEPGGAMLQRVRMKDALSLDERREWYVFAGVDARAVAYNVFLDGNLFRSSPSV
                     DRRNFVYDLKAGFSIRMAPARISLTQIWRSEEFTTPLAGGGTQRFQSLNLSWEF"
     gene            1089..2174
                     /locus_tag="VFY80_00315"
     CDS             1089..2174
                     /locus_tag="VFY80_00315"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HEX6006369.1"
                     /translation="MQFLDTIRQGSHHIWRSLPAGAIVRTLAFAATLVPCTSAVAAES
                     RGLFCATPTACELQFYVENDILGGTDRYYTSGLKLGGAVGAEGLVERFFHAPAERVLR
                     HITDDPRGVQFGLFIGQQLYTPREIDIAPPQPSDRPWAAWLYVGGVAQSVSANRLQTV
                     EFDLGVIGPAALGKEVQTAIHEATDSTHPKGWHNQLPNEPAFLLAYMEKWRFGPRSGV
                     QLVPHFGATLGTVLTLARIGATVRAGRNMSGFGPDTIEPGGAMLQGVRLRDPEYKEGI
                     GEWYLFAGADARAVAHNIFLDGNVFRSSPSVDRQEFVYDLKMGFSVRVASARVSFTHT
                     WRSEEFTTPVRGGGNQRFQSLNVSWQF"
     gene            complement(2373..>2513)
                     /locus_tag="VFY80_00320"
     CDS             complement(2373..>2513)
                     /locus_tag="VFY80_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6006370.1"
                     /translation="GIDPETGHYYDDTKRYLDAVSWLSAEEKERIYNGNIRKAYPRFV
                     AP"
BASE COUNT          437 a          802 c          817 g          457 t
ORIGIN      
        1 atttctcctc acacctgcag cgcatgctgc ggagccgtcg ctcatcgagt gcgatcgcgc
       61 aaccgcctgc gagctgcaga tctatatcga aaacgacagc gcgggagggg gaacggatcg
      121 ctactacacc aacggcatca aggtgggtgg cggcgtccac gccaatcggc tcatcgagcg
      181 ccttttccag cggccggctg aacaggtgct gcggcaattt tcggaaaaac cgggcgacgt
      241 gcacgtgggg cttttcatcg gccagcacat gtataccccg aagcgcatca ccgtcgcgga
      301 gccgcaaccc tttgaccggc cgtgggcagc gtggctttac gtgggcggcg ttgcgcaaag
      361 tgtcgcggga aatcgcctgc agaccgccga gttcgatctc ggcatgatcg ggccagcggc
      421 gctcggcaag gaaatgcaga cggcgtggca cgagctcgtc ggcgcggacc ggccggtggg
      481 ttggcacaac cagctcaaga acgagcccgg cctcatgctg acctacctcg aaaaatggcg
      541 tttcggccat ccgaccggta tggaactcgt gccgcacttc ggcggcagcg tcggcaacgt
      601 catgacgctc gcgcgggtcg gcggcatcgt gcgcgccggg aagaacatgt cgggtttcgg
      661 ccccgatacc atcgagccgg gcggcgcgat gctccagcgg gtgcgtatga aggacgccct
      721 gtcgcttgac gagcgccgcg agtggtatgt cttcgcaggg gtcgatgcgc gcgccgtggc
      781 ctacaacgtt tttctggacg gcaacttgtt ccgctcgagc ccgtcggtgg accgccgcaa
      841 tttcgtctat gacctcaagg cgggtttctc gatccgcatg gcgcctgcgc gcatctcgtt
      901 gacgcagatc tggcgcagcg aggagttcac tacaccgctc gcaggcggcg gcacccagcg
      961 cttccagtcg ctcaacctga gctgggagtt ctgaagcgca gaacgggata tgtccggctc
     1021 cgattgacga agtgaacggg gccgaaaggc tgctgtcgta ttgccagcct tttttgcgca
     1081 gccctcccat gcagttcctc gatacgatcc ggcaaggcag ccaccacatc tggcgctcac
     1141 tcccagcggg cgcaatcgtc aggacgctcg cattcgccgc gacgctcgtg ccgtgcacga
     1201 gtgcagtcgc ggccgagtcg cgtggtcttt tttgcgctac gccgaccgcg tgcgagctgc
     1261 agttctatgt cgaaaacgat atcctgggcg gcaccgatcg ctactacacg agcgggctca
     1321 agctgggcgg cgcggtcggc gccgagggtc tggtcgagcg cttcttccac gcaccggctg
     1381 agcgggtgct gaggcacatc accgacgatc cgcgcggcgt ccagttcggt ctgttcatcg
     1441 gccagcagct ctatacgccg cgcgagatcg acatcgcccc accccagccg tcggatcgtc
     1501 cgtgggcggc gtggctctat gtcggcggcg tggcacagag cgtgtccgcg aaccgcctcc
     1561 agaccgtgga gttcgatctc ggcgtgatcg ggccagcggc gctcggcaag gaagtccaga
     1621 cggcgatcca cgaagcgact gattcgaccc atcccaaggg ttggcacaac cagcttccga
     1681 acgagcccgc attcctctta gcctacatgg aaaaatggcg cttcgggccg cgcagcggcg
     1741 tgcagctggt gccgcatttt ggagccacgc tgggaacggt gctgacgctc gcccgtatcg
     1801 gtgcgacggt gcgcgccggg cgcaatatga gcggcttcgg cccggacacc attgagcccg
     1861 gtggcgcgat gctgcagggc gtgcggttgc gcgatcccga gtacaaggaa ggcatcggcg
     1921 agtggtacct cttcgccggt gccgacgcgc gcgcggtggc ccacaacatc tttctcgacg
     1981 gcaacgtgtt ccgctcgagc ccctcggtgg accggcagga attcgtctat gacctcaaga
     2041 tgggattctc ggtgcgcgtc gcttccgcgc gggtttcatt cacgcacact tggcgcagcg
     2101 aggaattcac caccccggtg agaggcggcg gcaaccagcg gttccagtcg ctcaacgtga
     2161 gctggcagtt ttgagcgtca gtgcgtcgac actcgcgcgc catcccggac cgctcctcgt
     2221 catcgcgact ttcgcacatc gtcatcccgg acactgatcc gggatccaga aaaaagcggg
     2281 catctggatt cgttggcgta cgccggaatg aggaaccgtg cgccggaatg acatgcaccg
     2341 gagccatacg tcgagaaggc acagccgagc acctacggcg cgacgaagcg cggataggcc
     2401 ttccggatgt tgccgttgta gatcctttcc ttctcttccg cgctgagcca gctcaccgcg
     2461 tcgagatagc gcttggtgtc gtcgtagtag tgcccggtct cgggatcgat gcc
//
LOCUS       DASURG010000019         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1128822, whole genome shotgun sequence.
ACCESSION   DASURG010000019 DASURG010000000
VERSION     DASURG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128822"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..>2031
                     /locus_tag="VFY80_00325"
     CDS             284..>2031
                     /locus_tag="VFY80_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6006371.1"
                     /translation="MPVGIAHFALDGRYLYVNPAFCELVGYTASELLARRVQDLTLPE
                     DVPEGETLQRNLAAGKISGFNREKRYRCKDGTIRWCSVAVRLVESKGAAKYFISVIDD
                     ISRRKEAENRFEATFNQAAVGIAHTTEEGRIIEVNRKLCDILGYSSEELVERSTRDLT
                     HPDDRDRQDALRIELLSGRRPFFSSEKRYVRKEGTVIWVNRTVTLAHRTASGKRYLIQ
                     VIEDITERKRTEQRLARVTRARRVMAECDRVLVHAKDEGTMLAEMCHIVVESGGYKMA
                     WVGLVTGDPRRPVYPAAHAGFGDDVPMSSPMGWSTDGRYRGFMNDAIRSGEPYIARDI
                     LSDPAHVRRRGRAVQHGFQSSIALPLKHQGAILGAAAIYAAEPDAFDAEELALLSELT
                     DDIAFGIASIRAVTARREVEAALHESERRFRDIFNHAAVGITRVDLAGVLVEVNQKFC
                     DMLRYSREELIGKSVSEITHPDDYGRGAQFRMQLADGEMRSVAGEKRFVRRDGSTIWT
                     RRTMSAACDEAGRPQYVISVVEDITERKKAEEALRANQQLLNATFSQAGVGIFASSLD
                     SRYIEVNEKYCSMLGY"
BASE COUNT          404 a          615 c          690 g          322 t
ORIGIN      
        1 tcataatgag tcgccgagtc gccgccagcc ggcgcgcccc ggggtagaac gcgcccagcc
       61 gggatggccg ctggcgggac gagaacaaaa ggggagtcga tccggcatga ggagaaaaac
      121 gaaggcgaag gccgtctccg cggcccgcgc gcggccgcgc gcggaaatga ggagcgcgcg
      181 tcggtcggga cgtccttctc ataccggccc cgagccggaa gaccgccgga aggcgcgtgg
      241 gccgtcacgc gcatcgtcca aagagcgcca tgctttcggt aacgtgcccg tcggcatcgc
      301 gcactttgcg ctcgatggtc gctatcttta cgtcaatccc gccttctgcg aactggttgg
      361 ctacaccgca tcggagctgc tcgcgcgccg ggtgcaggac ctgacgctcc cggaagacgt
      421 tcccgaagga gagacgctgc agcgcaatct ggccgccgga aagatcagcg ggttcaaccg
      481 cgagaagcgc tatcgctgca aggacggcac catacggtgg tgcagcgtcg cggtgcgact
      541 ggtggagtcg aagggcgcgg ccaagtactt catttcggtc atcgacgata tctcacgacg
      601 gaaggaagca gagaatcgct tcgaggcgac gttcaaccag gctgccgtcg gtatcgcaca
      661 cacgactgaa gaagggcgca tcatcgaagt caatcgcaaa ctgtgcgaca tcctcggcta
      721 ttcgtccgag gaactggtcg agcgatcgac gcgcgatctc acgcatcccg acgaccgcga
      781 ccgccaggat gcgctgcgca tcgagctcct ttcaggccgg agaccttttt tcagcagtga
      841 aaaacgctat gtgcgcaagg aaggcaccgt catctgggtc aaccgcacgg tcacgcttgc
      901 gcatcggact gcgagcggca agcgctatct catccaggtg atcgaggaca tcaccgagcg
      961 caagcgcacc gagcagcggc ttgcacgcgt gacgcgcgca cggcgcgtca tggccgaatg
     1021 cgaccgcgtg ctggttcacg cgaaggacga ggggacgatg ctcgcggaga tgtgccacat
     1081 cgtcgtggag tcgggcggat acaagatggc atgggtcggg ctggtgaccg gagacccgcg
     1141 gcgcccggtc tatccggccg cgcatgccgg attcggggac gacgtgccga tgtcgagccc
     1201 catgggctgg agcacggacg gccgttaccg ggggttcatg aacgacgcaa tcaggagcgg
     1261 tgaaccttac atcgcccgcg acattctgag cgatcccgca cacgtgcgcc gccgggggcg
     1321 cgcggtccag catggattcc agtcgtcgat cgcgctgccg ctcaagcatc agggggcgat
     1381 acttggcgcg gctgcgatct atgcggccga gcccgacgcc ttcgacgccg aggagctcgc
     1441 actactgagc gagctgaccg acgatatcgc gttcggcatt gctagcatcc gggcggtcac
     1501 cgcacgccgg gaagtcgagg cggcgttgca cgagagcgag cgccgctttc gtgacatctt
     1561 caaccacgca gccgtcggca tcacccgggt ggatctcgcc ggcgtgctgg tcgaggtgaa
     1621 ccagaaattt tgcgacatgc tgcgttattc gagagaggag ctgatcggca aatcggtgag
     1681 cgagattacc catcccgacg attatggccg cggcgcgcag ttccggatgc agctcgccga
     1741 tggagaaatg aggtcggtgg cgggagagaa gcggttcgtt cgccgcgacg gctcgacgat
     1801 atggacgcgg cggacgatgt cggcggcctg cgacgaagcc gggcgtcccc agtacgtcat
     1861 cagtgtcgtg gaggacatca ccgagcgcaa gaaagccgag gaggcgctgc gcgcgaacca
     1921 acaactgttg aacgcgacgt tcagccaggc gggggtcggc attttcgcga gctcgctcga
     1981 ttcacgctat atcgaggtca acgaaaagta ctgctccatg ctgggataca g
//
LOCUS       DASURG010000020         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_413942, whole genome shotgun sequence.
ACCESSION   DASURG010000020 DASURG010000000
VERSION     DASURG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413942"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..531)
                     /locus_tag="VFY80_00330"
     CDS             complement(310..531)
                     /locus_tag="VFY80_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006372.1"
                     /translation="MIVPETTAREGADYRREKPMPSMERLSFTPGKGNTADLDVRLLS
                     DQDLQQALEEGRKRKPRVRPPTPDPSFDG"
     gene            701..2398
                     /locus_tag="VFY80_00335"
     CDS             701..2398
                     /locus_tag="VFY80_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6006373.1"
                     /translation="MLRQIASIPALAMVVAVSFALPQGSKANSGSHHQTKSTAHNTAG
                     RVPLYDNLGNHEYRVTTRSDLAQRYFNQGLRLYYAFNHQEAIRAFEEAARLDPTCAMA
                     HWGIALALGPNINLPMDEKAGHAAYAAIRKALELQSNASAKERALIAALAKRYAADPR
                     QDRAALDKAYATAMREVVRRHGDDLEARTLYAESLMDLSPWNYWTREGKPREHTKTLL
                     EQLEHVIAANPNHPGANHFYIHAVEAVQPERAVPAAERLAQLMPGAGHLVHMPGHIYI
                     RVGRYLDAIKANEHAVHADESYIRDQNPAFGVYVAGYYPHNYDFLAFAASMIGRSTQA
                     IGAAEKIATLATPDIMKAPGMTFAQHHNTRHLQMKVRFARWDDILKAPAPSADLPHAR
                     AMWHYARGRALAARGDASAAEAELGALRTAAADKSLAQARLEFNASTDVLAIAGVVLA
                     GHIAAARGDHKAAIAHLRKAARQEDQLVYGEPPEWSIPVRQELGVVLIKAGQAREAER
                     AFREDLKRFPDNGWSLHGLTLALEAQNRTAEARDVRKRLGKVWSDADVPLKFAGAHQ"
     gene            2440..>2692
                     /locus_tag="VFY80_00340"
     CDS             2440..>2692
                     /locus_tag="VFY80_00340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6006374.1"
                     /translation="MTGTRKDTWAAGERYEPYVGRWSRLVAREFLAWLQAKPMLDWLD
                     IGCGTGALTETVLRESQPRSVKGVDPSAGFVEHARARIRD"
BASE COUNT          515 a          847 c          888 g          442 t
ORIGIN      
        1 cgatgccttc catggagcgc ttgagcttca cgccgggaaa gggcaacacc gccgacctcg
       61 atgtgcgcct gctgtcggac caggatctgc agcaagcgct cgaggaaagg cgaaagcgga
      121 agggatgaga tgctcaaact cgacgacgac gacgcgcgcg ttctcatgaa gcgcatcgcc
      181 aaggcgctcc gcaaggcgcg ctaccaccct gcggaggtca cggacgaaga gtcggccgca
      241 ttcgaggtga ttcgtccgta tgtttcgcgg gaggtcgtgc gatcccgcgg cgctccgcgt
      301 gcggaccaat cagccgtcga agctaggatc cggcgttggc gggcgcactc ggggcttccg
      361 ctttcgccct tcctcgagcg cttgctgcag atcctggtcc gacagcaggc gcacatcgag
      421 gtcggcggtg ttgccctttc ccggcgtgaa gctcaagcgc tccatggaag gcatcggctt
      481 ttcccggcga tagtcggcgc cttcgcgtgc agtcgtttcg gggacgatca tgtgcgtgcc
      541 tcctaaaccc tgatagagca cgccgcgccg ggcagggttc cgtccccgat acaaagccga
      601 aacaattcga aaccgccggg acatcgtcat cgtcccggcg tgcgcttacc attgcgccac
      661 gaatgttttt ccgagtgtat tgttcaggaa ggagagtgcc atgttgagac aaattgcttc
      721 gatcccggcc ctcgcgatgg tggtggcggt ttcgtttgcg ctgccgcaag gttccaaggc
      781 caattccggt tcacatcacc aaacgaagag caccgcccac aataccgcgg gccgggtgcc
      841 gctttacgac aatctcggca atcacgaata ccgcgtcacg acacggagtg atctcgcgca
      901 gcgctatttc aaccagggac tgcgcctcta ctacgcgttc aatcatcagg aagcgatccg
      961 tgctttcgag gaagcggcgc gactcgatcc gacctgcgcg atggcgcact ggggcatcgc
     1021 gcttgcgctc gggcccaaca tcaatctgcc gatggacgag aaagcgggtc acgcggccta
     1081 tgccgcaatc cgcaaggcgc tcgagctgca gtcgaacgcg agcgcgaaag agcgcgcgct
     1141 gatcgcggcg ttggcgaagc gctatgccgc cgatccgagg caggaccgcg ccgcgctcga
     1201 caaagcctat gcgacggcga tgcgcgaggt ggtgcgccgg catggcgacg acctcgaggc
     1261 gcgcacgctc tatgccgagt ccctgatgga tctgagtcca tggaactact ggacacgcga
     1321 aggcaagccg cgcgagcaca cgaaaacact cctcgaacag ctcgagcatg ttatcgctgc
     1381 caacccgaat catccgggtg cgaaccactt ctacatccat gccgtggagg cggtgcagcc
     1441 cgagcgtgcg gtgcccgcgg cggagcggct cgcgcagctc atgccgggcg cggggcatct
     1501 cgtgcacatg ccgggacaca tctacatccg ggtgggacgc tatctcgacg ccatcaaggc
     1561 gaacgagcac gcggtgcatg ccgatgaaag ctacatccgc gaccagaacc cggcattcgg
     1621 cgtttatgtc gcaggctact atccgcacaa ttacgacttc ctcgcgtttg cggcgagcat
     1681 gatcggccgc agcacgcagg cgatcggcgc agccgaaaaa attgcgacgc tcgccacgcc
     1741 ggacatcatg aaagcgccgg ggatgacgtt cgcgcagcac cacaatacgc ggcacctgca
     1801 gatgaaggtg cggtttgcgc gctgggatga catcctgaag gcacccgcgc cgtcggccga
     1861 tcttccccac gcccgggcga tgtggcatta cgcgcgcggc cgggcacttg ccgcacgagg
     1921 cgacgcgagc gctgcggaag ccgagctcgg tgcgttgcgg accgcggccg cggacaagag
     1981 cctcgcgcag gcgcggctgg agttcaatgc gtccacggac gtgctcgcca tcgcgggtgt
     2041 agtgctcgcg ggtcacatcg ccgccgcaag aggtgatcac aaggcggcga tcgcgcatct
     2101 gcgcaaggcg gcacgccagg aagaccagct ggtctacggt gagccgcccg aatggtcgat
     2161 tccggtacgc caggaactcg gcgttgtcct catcaaggca ggacaggcgc gcgaggcgga
     2221 gcgcgcgttt cgcgaagatc tgaagcgctt tccggataat gggtggtctc tgcacggcct
     2281 cacgcttgca ctcgaagccc agaaccggac ggcggaagcg cgcgatgtca ggaagcgcct
     2341 cggcaaagta tggtcggatg cggatgtgcc gctcaagttc gccggggcgc accaataaga
     2401 cgtatcctag cctccacgac acacgaggag gcggatgcga tgacaggcac gcgcaaggat
     2461 acctgggcgg caggtgaacg gtacgagcct tacgttgggc gctggagtcg gctcgtcgcg
     2521 cgcgagttcc tcgcgtggct gcaggcgaag cccatgctcg actggctgga catcgggtgt
     2581 ggcactggtg cgctcaccga aaccgtgctg cgggaatcgc agccgcggag cgtgaagggc
     2641 gtggaccctt ctgccgggtt cgtcgagcac gcgcgtgcgc gcattcgtga cg
//
LOCUS       DASURG010000021         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_209830, whole genome shotgun sequence.
ACCESSION   DASURG010000021 DASURG010000000
VERSION     DASURG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209830"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1228
                     /locus_tag="VFY80_00345"
     CDS             44..1228
                     /locus_tag="VFY80_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX6006375.1"
                     /translation="MNYPLRLYSGKDALENLPAEMKRQRATRALVVCGRTVSRKTGLI
                     QRIRTILGEACAGVFDEMEKESPLKAILRARDAARVANADLLIGVGAGSVIQATRVVA
                     ILLAEKRPVEELITQYPEQGPAISPKLLEPKLPIINVLTAATSAQNRAGSAVKSETVD
                     HRMEFFDPKTRPVAVFWDADALLTAPATLARDTGAAVLWRAVMNLGATRMNPLVEGDR
                     LQAFRLARDATARLAEANDAGPRIDLCAAAFLQNRDADEGGTMFERHWAGRVIYAFAA
                     ALFNVHPHVSQGAANCALSGTVIRRLGARNLPEMARIAQALGAWNGSEPLKQAPQRAA
                     DSLDALFRSIGMPTRVGELDVPKESVDRMLEHALKNFNADPKREFVRERDVLREVLLA
                     AW"
     gene            1258..1659
                     /locus_tag="VFY80_00350"
     CDS             1258..1659
                     /locus_tag="VFY80_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6006376.1"
                     /translation="MDGLKVGMKHSLEWEVTENRCTTRGDYKVFSTPSMTQFVEMTAQ
                     ALAAPYLKPGQGQVGVSVNIRHLAPTPIGKKVRADAELVGIDRRRLTFRVKVFDDVEQ
                     VGEAEHERFVIDVDKYIERLRKKIGASPSST"
     gene            complement(1704..2702)
                     /locus_tag="VFY80_00355"
     CDS             complement(1704..2702)
                     /locus_tag="VFY80_00355"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HEX6006377.1"
                     /translation="MDPQDRRLFTALMILVLLLVGAYLAMIATASFAATGTAAKAATK
                     QRSFASAEQAMEALAAAVQAHDVAQLRAIFGPGGETILQSGDPLEDKETRGRFTAAYA
                     ESHKLEQRGESTAWIVVGKDEWPLPIPVVKRGESWTFDTRAGKEEVLNRRIGRNELSA
                     AQAALAYVDAQVEYYLRNPQNDKLLQYAQRFVSSAGKRDGLYFPTNAGEKPSPLGPLF
                     DARRAAGYSPEAGGKPGPYHGYYYRILKAQGPKAAGGAYQYVVNGKMIGGFALVAYPA
                     TYGNSGVMTFMVNHEGVVYEKNLGPDTVAIAQKMTRFNPDETWARVSPEAHTASAR"
     gene            complement(2732..>2957)
                     /locus_tag="VFY80_00360"
     CDS             complement(2732..>2957)
                     /locus_tag="VFY80_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006378.1"
                     /translation="TGDGGFGDGRSGGGVGDRRESVARPTGGRDAGAFQGVGGRGAET
                     RSFSDRGAASRASAGGGARAGGGARGGGRR"
BASE COUNT          495 a          984 c          965 g          513 t
ORIGIN      
        1 cacgcggagt cggcgctaag tgcatatctg cgatttccgc cttgtgaatt acccgctgcg
       61 cttgtacagc gggaaggacg cactggaaaa tcttccggcg gagatgaagc gtcagcgggc
      121 tacgcgggcg ctcgtcgtct gcggccgcac cgtctcgcgc aagacgggac tcatccagcg
      181 gatacgcacg atactcggtg aagcgtgcgc aggcgtcttt gatgagatgg agaaggaatc
      241 gccgctgaag gcgatcctgc gcgcgcgcga tgccgcccgg gtagcgaatg ccgatcttct
      301 gatcggcgtg ggcgccggaa gcgtgatcca ggcaacgcgc gtggtggcga tcctgctcgc
      361 ggagaagcgt ccggtcgagg agttgatcac ccagtatccg gagcaggggc ccgcgatcag
      421 tccgaagctg ctcgagccga agcttcctat catcaacgtg ctgacggctg cgacctcggc
      481 ccagaaccgc gccggctcgg cggtgaaaag cgagacggtg gatcaccgca tggagttctt
      541 cgatcccaag acgcgcccgg tcgcggtctt ctgggacgcc gatgcgctgc tcactgcacc
      601 cgcgacgctc gcgcgcgata ccggcgcggc ggtgctgtgg cgtgccgtga tgaatctcgg
      661 tgcaacgcgc atgaacccgc tcgtcgaagg cgaccggctg caggcgtttc gccttgcgcg
      721 cgacgcgacg gcgcggcttg cggaagcaaa cgacgccggc ccccgcatcg atctgtgcgc
      781 ggccgcgttc ttgcagaacc gcgacgcgga cgaaggcggc accatgttcg aacgccattg
      841 ggcgggccgc gtgatctatg ctttcgcggc ggcgcttttc aatgtccacc cgcacgtaag
      901 ccagggtgcg gcgaactgcg cgctgagcgg caccgtcatc cgcaggctcg gcgcgcgcaa
      961 tctgcccgag atggcgcgca tcgcgcaggc gctgggcgcg tggaacggtt cggagccgct
     1021 caagcaggcg ccgcaacgcg cagccgattc cctcgatgcg ctcttccgca gcatcgggat
     1081 gccaacccgg gtgggcgaac tggatgtccc gaaggagagc gtggaccgca tgctcgagca
     1141 cgccctcaag aacttcaatg cggacccgaa acgcgaattc gtgcgcgagc gtgacgtcct
     1201 gagggaagtg ctgctcgcgg cgtggtagaa gtcgaagtca gggaagggat ctgaaaaatg
     1261 gacggactca aggtcgggat gaagcactcc ctcgagtggg aggtgacgga gaatcgctgc
     1321 actacccgcg gggactacaa ggtgttctcc acgccttcga tgacgcagtt cgtggagatg
     1381 acggcgcagg cgctcgccgc gccttatctc aagccgggcc aggggcaggt cggcgtctcg
     1441 gtcaacatcc gtcacctcgc gcccacgccg atcgggaaga aggtccgtgc ggatgccgaa
     1501 ttggtcggca tcgaccgccg caggctcacg ttcagggtca aggtcttcga tgacgtcgag
     1561 caggtcggtg aagcggagca cgagcgcttc gtgatcgacg tggacaagta catcgagcgc
     1621 ctcaggaaga aaatcggcgc ttcgccatcg tcgacttgat gagaggcacg ggtcgcgcgg
     1681 ccaccgcgtc ccgactcgcc cactcaacgc gctgacgcgg tgtgcgcctc cgggctcaca
     1741 cgcgcccacg tctcgtccgg attgaatcgc gtcatcttct gagcgatggc gacagtgtcc
     1801 ggccccagat tcttctcgta gacgacgccc tcgtggttca ccatgaatgt catgacgccg
     1861 gaatttccgt aggtcgcggg atatgccacc agggcgaacc ctccgatcat cttgccgttg
     1921 acgacatact gataggcgcc gccggctgct ttagggccct gcgctttcag gatcctgtag
     1981 taatagccgt gatagggccc cggcttgccg ccagcctcgg gagagtagcc cgcggcgcgt
     2041 cgggcatcga agagcgggcc gagcggactc ggcttttcgc ccgcattggt cggaaaataa
     2101 aggccgtcgc gcttccccgc actgctcaca aaccgttggg cgtattgcag cagcttgtcg
     2161 ttctggggat tgcgcagata gtactccacc tgggcgtcca cgtatgcgag cgccgcctgc
     2221 gccgctgaca gctcgttacg gccgatgcgg cggttgagca cttcctcctt gcccgccctg
     2281 gtatcgaacg tccagctttc gccccgcttc actacgggga tcggaagcgg ccattcgtcc
     2341 ttgcccacga cgatccacgc agtcgactcg ccgcgctgct cgagcttatg tgactccgca
     2401 taggcggctg tgaatcgccc gcgcgtctcc ttgtcttcta gcgggtcgcc cgattggagt
     2461 atggtttctc cgcccgggcc gaagatcgcc ctgagctgtg caacgtcatg cgcctggacc
     2521 gccgcagcga gcgcctccat cgcctgctcg gcgctcgcga aggatctctg ttttgtcgca
     2581 gccttcgctg ccgtgccggt tgcagcaaag ctcgcggtgg cgatcatcgc caagtaggcg
     2641 ccgaccaaca gcagtacgag aatcatgagc gccgtgaaca ggcgcctgtc ctgcgggtcc
     2701 atttcggttg cctccgcccc tgaccgcttc atcatcgccg gcctccaccg cgcgcaccgc
     2761 ccccggcgcg cgcaccgccg ccggcggaag cgcggctcgc cgcaccgcgg tcgctgaagc
     2821 tgcgcgtctc cgctcccctg ccgcccacgc cctgaaatgc gcccgcatcg cggcctccag
     2881 tgggtcgcgc gacggattcg cggcgatcgc ccaccccgcc gccactgcgc ccgtcgccga
     2941 agccgccatc gcccgtg
//
LOCUS       DASURG010000022         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_720338, whole genome shotgun sequence.
ACCESSION   DASURG010000022 DASURG010000000
VERSION     DASURG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720338"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1239)
                     /locus_tag="VFY80_00365"
     CDS             complement(43..1239)
                     /locus_tag="VFY80_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HEX6006379.1"
                     /translation="MKQFRIPAVFMRGGTSNAVVFHARDLPRERALWDEIFLAAIGSP
                     DPYGRQLDGMGGGISSLSKVCVVGPASRADADIDYTFAQVQVTDAKVDYSGNCGNMSS
                     AMGPFAVDEGLVKASGQEALVRIHNTNTRKIIWSRFALDEGMSAVDGDLAIPGVSGTG
                     APVRLEFREPGGATTGKLLPTGNVVDALDVPGYGKVMASLVDAANATCFVSATDLGLK
                     GTEMPEELDRNAELLKKLAAIRIAASVAMGIGRTPEEAAEKRTVPFVGFVSAAQDAMT
                     LSGERIAGSGVDLTARMLSNGQPHRALPLTVTLCTAVAARIEGTLVNRLTRRRDDPEA
                     EIRIAMPSGVLTVAATVTRKSGEWHAEQGAFYRTQRRMFEGHVLVRASRVPGFVAATG
                     GRLKAA"
     gene            complement(1292..>2762)
                     /locus_tag="VFY80_00370"
     CDS             complement(1292..>2762)
                     /locus_tag="VFY80_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6006380.1"
                     /translation="YRSDRLELPRTRRGPSRDAFRRTACGAVMLTHELCEIIAATRFE
                     SLGAECVLRVKQAIKDGVAVAMAGSREQPIELLAAHLKSLGGKPQSTVWGAGYRTSPV
                     HAAYANGAATHVLDFEPMWLPPTHAVSPVVPAAFALAEALGIGGREVVTAVAKGMEIQ
                     GRVQYAANQFKPEKLRFHPPGIAGVLGAAVTAAHLLGLDSDRIAHALGIAASRAGTLL
                     ANIGSMTKSTHCGYAAASGVDAAMLASRGFTAHPAVLEAPRGFIETFYPDDFDRDRLL
                     AYGKPFRIVEPGLAIKRFPSQFATHWAITAGLELHPKIQDPARIGRVVIRTPVMRYCD
                     RPQPHNGHEGKFSFQYAAAVALLDGCVTIASFSDPRRFRSDMVALLGKTELVQDPTIP
                     GEWVGMHVTIEVEMRDGSTLRTTCHGPRGAWGQPPLSPVEHEEKLRDCLDRALHASRI
                     GRLLARLDQLDVQTARGVATLMQSLAGRRARARAANRKR"
BASE COUNT          458 a          943 c          883 g          478 t
ORIGIN      
        1 cgatcgacga tcgaggcgcg gtgcgcggcg ccgcgctacg gactacgcgg ctttgagcct
       61 gccaccggtt gcggcaacga agcccggcac gcgcgaggca cgcacgagca cgtgcccttc
      121 aaacatccgc cgctgtgtgc gatagaacgc gccctgctcg gcgtgccatt cgccgctctt
      181 tcgcgtcacc gtggccgcaa cggtgagcac gccggaaggc atggcgatgc gaatctccgc
      241 ctccggatcg tcgcgacggc gggtgagtcg attcacgagc gtgccctcga tgcgcgcagc
      301 caccgccgta cacagcgtga cggtgagagg cagtgcgcga tgcggctgac cgtttgacag
      361 catgcgcgca gtgagatcga cgccgcttcc cgcgatcctc tctcccgaca gcgtcatcgc
      421 gtcctgagca gcgctcacga aaccgacgaa cggcacggtg cgcttttcgg cggcctcttc
      481 cggtgtccta ccgatcccca tggcgaccga cgccgcgatg cggatcgccg cgagcttttt
      541 cagaagctcg gcgttgcggt cgagctcttc cggcatttcg gtgcctttga gtccgaggtc
      601 cgttgcgctg acaaagcagg tggcgttcgc cgcgtcgacg agcgaagcca tgactttccc
      661 gtaaccgggc acatcgagcg catcgacgac gttgccggtc ggcagaagct tgccggtcgt
      721 cgcgccgccc ggctcgcgga attcgagccg caccggcgcg ccggttcccg atacgcccgg
      781 tatcgcgaga tcgccatcca cggcggacat cccctcgtcg agcgcaaaac gcgaccagat
      841 gattttcctg gtgttggtgt tgtgaatgcg caccagcgct tcctgcccgg acgccttgac
      901 gagcccttcg tccaccgcga acggccccat cgccgaggac atgttgccgc aattgccgct
      961 gtagtccacc ttggcgtccg tcacctgcac ctgcgcgaag gtgtaatcga tatcggcatc
     1021 cgcgcgcgac gcgggtccga ccacgcatac tttggacagc gaagaaatcc cgccgcccat
     1081 gccgtcgagc tggcgcccgt acggatccgg actgccgatc gccgcgagaa agatctcgtc
     1141 ccacaatgcg cgctcgcgcg gaagatcgcg cgcgtggaac actaccgcgt tgctggtgcc
     1201 gccgcgcata aacactgcag ggatcctgaa ctgcttcatc gcctgtctcc tatcgtccgc
     1261 atcccgccgg tatgtggagc tattgtagtc ttcatcgctt cctattcgcg gctcgcgctc
     1321 gcgcacgcct gcccgcgagc gactgcatga gcgtcgccac gccgcgcgcg gtttgcacgt
     1381 ccagctgatc gagccgcgcg agcagccgac cgatgcggct ggcgtgaagc gcacggtcga
     1441 ggcagtcgcg cagcttttct tcgtgctcga cggggctgag cggcggctgg ccccatgcgc
     1501 ctcgcggtcc gtggcaggtc gtccgaagcg tcgagccgtc ccgcatttcg acttcgatcg
     1561 tcacatgcat gccgacccat tcgccgggga tggtcggatc ctgcacgagt tccgtcttgc
     1621 ctaggagcgc caccatgtcc gaacggaagc gccggggatc gctgaagctc gcgatggtaa
     1681 cgcagccgtc gagaagcgca accgcggccg catactgaaa gctgaacttg ccttcgtgcc
     1741 cgttgtgggg ctgcgggcgg tcgcagtagc gcatgaccgg cgtgcggatc acgacccgcc
     1801 cgatccttgc gggatcctga atctttggat ggagctccag tcctgccgtg atcgcccagt
     1861 gcgtcgcgaa ctgggaggga aaacgcttga ttgcgagacc cggctcgacg atgcgaaacg
     1921 gcttgccgta tgcaagcagc cggtcgcggt cgaaatcgtc gggatagaac gtctcgatga
     1981 atccgcgcgg agcttccaag acggcgggat gcgccgtaaa gccgcggctc gcgagcatgg
     2041 cggcatcgac gcccgaagcc gcagcgtagc cgcagtgcgt gctcttcgtc atcgacccga
     2101 tattcgcaag cagtgtaccc gcgcgcgacg cggcgatgcc gagtgcgtga gcgatccgat
     2161 cgctgtccag tccgagcagg tgagcggcgg tcacggccgc gcccagcaca ccggcgatgc
     2221 caggcggatg aaaccgcagt ttctcaggct tgaactggtt cgccgcgtat tgcactcgcc
     2281 cctggatctc catccctttg gcgaccgcgg tcacgacttc gcggccgccg atgccgagcg
     2341 cttcggcgag cgcgaaagca gcgggaacga cgggcgaaac cgcgtgcgtc ggcggcagcc
     2401 acatcggctc gaagtcgagc acatgcgtcg ccgcgccgtt cgcataggcc gcgtgaaccg
     2461 gcgaggtcct gtacccggcg ccccagacgg tggattgcgg cttgccgccc aggcttttca
     2521 aatgcgcagc gagcagttca atcggctgtt ccctgctgcc cgccatcgca acggcgacgc
     2581 catccttgat cgcctgcttc acgcgcagca cgcactcggc gccgagcgac tcgaaccgcg
     2641 tcgcggcgat gatttcgcac agctcgtgcg tcaacatcac ggcaccgcac gccgtgcgac
     2701 ggaaggcatc acgactcggt ccgcgtctcg tgcgaggcag ttcgagtcta tccgatcgat
     2761 ac
//
LOCUS       DASURG010000023         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_312803, whole genome shotgun sequence.
ACCESSION   DASURG010000023 DASURG010000000
VERSION     DASURG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312803"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1334)
                     /gene="prfB"
                     /locus_tag="VFY80_00375"
     CDS             complement(join(227..1252,1254..1334))
                     /gene="prfB"
                     /locus_tag="VFY80_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095522732.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX6006381.1"
                     /translation="MEAERVNAIEKRLQDVAGRTAALRRYLDFDAKAKRLAEVARLIE
                     DPAVWDDNKRAQELGKERKALEAVVATLSRLDQDIRDASELFEMARSESDDVVLLGIA
                     RDAETLERTVADMEFRRMFSNPLDPNNCFMDIQAGSGGTEAQDWAAMLERMYLRYCDR
                     KRFRAEVLEESPGEVAGIKSATIKVTGDYAYGHLRTETGVHRLVRKSPFDANNRRHTS
                     FASVFVYPEADETIEIEINPADLRIDTFRASGAGGQHVNKTDSAVRITHLPTNIVVQC
                     QNDRSQHRNRAEAMAMLKSRLYELELRKREQEKKALEESKTDIGWGHQIRSYVLDQSR
                     IKDLRTNVEIGNTQAVLDGDLDDFISASLKQGTT"
     gene            complement(1392..2438)
                     /locus_tag="VFY80_00380"
     CDS             complement(1392..2438)
                     /locus_tag="VFY80_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006382.1"
                     /translation="MQCTLLIPHLAPRADLLDAPGGGPDLPNLQLLMSRGRHRAFPPL
                     AWEAWLCQAFEVERQRDWPVAPLTLAADGGDPEESYWLRADPVHMRIHRNQLVLSDTS
                     GFAVSLEEARALVESMNAHFKPEGLRFSAPHANRWYLRVDNDPGVSTTALDDAAGDLI
                     DRHLPAGPNALAWRRILNETQMLLHAHAINEAREARGEPAINGVWFWGGGRRTAVPGR
                     HFTAVVSNEALGLALAAHADVTASPLPRNAVQWLAARSEHGAHRLVVPDALTASARRG
                     DAVAWRRALEALDADWIGPLIQALKAGRLAEVALVAPAPLACHRFELRRRDLLRFWRM
                     PRPPARWLEEPSRV"
     gene            2534..>2975
                     /locus_tag="VFY80_00385"
     CDS             2534..>2975
                     /locus_tag="VFY80_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6006383.1"
                     /translation="MRAYELFIGLRYTRAKQRTRFISFISIISVVGMALGMMVLITVL
                     SVMNGFQREIRTRILGVASHVQVSGPNNMLENWREVAAQAARHEQVVAVAPYVSAQGL
                     VTHGSAVRGVFVRGILPEQEDKVAEFDKHMRSGRLDALRAGEYGV"
BASE COUNT          519 a          926 c          953 g          577 t
ORIGIN      
        1 gcgagtttcg cacgacgttc ctcgatgatc gggttcgtct cctcgacggg cgcgggccgc
       61 tgatcgtcca tgtgggaatc cataggaatg cctacctaat atgccgtccc ataactacac
      121 gtcgtcattc cggcggaagc cggaacccgg aaaatgtgcg actcgatgcc ggttttcgcc
      181 ggcatgacgc atctcggatc agtcaggtta tcaatgggat gccacactag gtcgtgccct
      241 gcttcaggct cgcgctgatg aagtcgtcga gatcgccgtc cagcaccgcc tgcgtattgc
      301 cgatctcgac gttggtccgg agatccttga tgcgcgactg atcgagcaca taggaccgga
      361 tctggtgtcc ccaaccgata tcggtcttgc tctcttccag cgccttcttt tcctgctcgc
      421 gcttgcgcag ttcgagctca tagaggcgcg atttcaacat cgccatggcc tcggcgcggt
      481 tgcgatgctg cgagcggtcg ttctggcact gcacgacgat gttggtcggc aggtgcgtga
      541 tgcgcactgc ggagtccgtc ttgttgacgt gctggccacc ggcgccggaa gccctgaagg
      601 tgtcgatgcg aagatccgcc ggattgattt cgatttcgat cgtctcgtcg gcctcgggat
      661 agacgaacac gcttgcaaag gacgtgtggc ggcggttgtt ggcgtcgaaa ggtgacttac
      721 ggacgaggcg atggacgccg gtctcggtgc gcagatgccc atacgcatag tcgccggtca
      781 ctttgatcgt ggcgctcttg atgcctgcca cttcgccggg cgattcctcc agcacttcgg
      841 cgcggaagcg cttgcggtcg caatagcgca ggtacatccg ttccagcatc gcggcccaat
      901 cctgggcctc ggtaccgccc gagccggcct ggatgtccat gaagcaattg ttggggtcca
      961 gcgggttcga aaacatgcgg cggaattcca tgtccgccac ggtgcgttcg agcgtttcgg
     1021 cgtcgcgtgc gattccgagg agcacgacgt catcggactc actgcgcgcc atttcgaaaa
     1081 gctcggaagc gtcgcggatg tcctggtcga ggcgcgacag cgtcgcaacg accgcttcga
     1141 gtgccttgcg ctccttgccc agttcctgcg cgcgcttgtt gtcgtcccag accgctggat
     1201 cttcgatcag cctggcgact tctgcaaggc gcttcgcctt ggcatcgaag tcaaagatac
     1261 ctccgcagcg ctgccgtgcg gccagcgacg tcctggaggc gcttctctat ggcattgacg
     1321 cgttctgctt ccattcacga cacacattcg cgagaaaggc ggattataca cagcatcaac
     1381 cgatagccgc gtcaaacgcg tgacggctct tcgagccagc gcgcgggtgg gcgcggcatg
     1441 cgccagaagc gcagcagatc gcgccgccgc aattcgaacc ggtggcaggc taagggcgcc
     1501 ggcgcgacca aagcgacttc agcgagcctg ccagccttga gtgcctggat cagcggtccg
     1561 atccaatcgg cgtcgagcgc ttccagagcg cggcgccatg ccaccgcgtc accgcgtcgt
     1621 gcagaagccg tcagcgcgtc gggcacgacg agacgatggg cgccgtgctc ggaacgcgcc
     1681 gcgagccact gcaccgcgtt tcgtgggagc ggagaggccg tgacgtcggc atgcgcggca
     1741 agagccaaac cgagcgcctc gttcgacacg accgccgtga agtgccgtcc cggcaccgct
     1801 gtgcgacgcc caccgcccca gaaccacacg ccgtttatcg ctggctcacc gcgtgcttcg
     1861 cgcgcttcgt taatggcgtg cgcatggagc agcatctggg tttcgttcag gatgcgacgc
     1921 caagcaagtg cattcggacc ggcgggcaga tggcgatcga tgagatcgcc tgcagcatcg
     1981 tccagcgcgg tggtgctcac tcccggatcg ttgtctacac ggaggtacca gcgattcgca
     2041 tgcggcgcgc tgaagcgcag gccttccggc ttgaaatgcg cattcattga ttcgacgagg
     2101 gcgcgcgcct cctcgagcga aacggcaaag ccgctcgtgt cggagagcac cagctgattg
     2161 cggtggatgc gcatgtgcac ggggtctgcg cgcagccagt agctctcctc aggatcgccg
     2221 ccatcggccg caagggtgag cggcgcaacg ggccagtcgc gctgacgttc cacttcgaag
     2281 gcttggcaca accatgcctc ccaggcaagc ggaggaaacg cgcggtggcg cccgcgcgac
     2341 atgagaagtt gcagattggg gagatccggg cctccacccg gcgcgtccag taagtcggcg
     2401 cgcggggcaa gatgcggtat cagtaacgtg cactgcattt cactgtcatc ctgaatcact
     2461 gtatcacacg gagcgcgcgt gctttgacga ggccattgtg gcaaactgcg cctttcaatt
     2521 tatcccgcct cccttgcgcg cctacgagct tttcatcggc ctgcgctaca cccgcgcaaa
     2581 gcagcgcacg cggttcatct ccttcatctc catcatttcg gtcgtcggca tggcgcttgg
     2641 catgatggtg ctgatcacgg tgctctccgt catgaacgga tttcagcgcg aaattcgcac
     2701 gcgtatcctc ggcgtcgcgt cgcatgtgca ggtgagcggg cccaacaaca tgttggagaa
     2761 ctggcgcgag gtggcagctc aggcggcgcg ccacgagcaa gtggttgccg tagcgcctta
     2821 cgtcagcgcg caggggctgg tgacgcacgg ctcggcggtg cgcggcgtct tcgtgcgggg
     2881 aattttgccg gagcaggaag acaaggtcgc cgagtttgac aagcacatgc gaagcggccg
     2941 cctcgatgcg ctgcgtgccg gcgagtacgg cgtca
//
LOCUS       DASURG010000024         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1436802, whole genome shotgun sequence.
ACCESSION   DASURG010000024 DASURG010000000
VERSION     DASURG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436802"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFY80_00390"
     CDS             <1..554
                     /locus_tag="VFY80_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX6006384.1"
                     /translation="MAKSAELGDLTLAANQVLHNFLVFTSFGLDGFAHAAEAILGEAV
                     GRRDRAAFRHSMRVVFFWAALVGALNLVLYAVAGRGIIALLTNIPEVRTAAGEFLLWP
                     VIMPVVSVWAFTYDGVYLAATRTTIMRNTVIAAFVAFIVVLHVATPIYGNLGVWIAVT
                     VFLGLRGLLLHLFFSRTLRAFGV"
     gene            complement(674..1231)
                     /locus_tag="VFY80_00395"
     CDS             complement(674..1231)
                     /locus_tag="VFY80_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255440908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(983..985),aa:Sec)
                     /transl_table=11
                     /product="OsmC-related (seleno)protein"
                     /protein_id="HEX6006385.1"
                     /translation="MKPLGNEDLSERLGERVIDRPELPAAGITNENDVLTEMVTGELQ
                     LKRGTSGKFEVYCDEPSRIGGTDRYPSPMTYLAMAIGFULLTQVARYAHMMKMKVTGA
                     SCRVVMRKHLGGSVLKGTVFNRWEGISTHLKLESEEPEERLAHLVKNAKAGCFAEGLI
                     TQQVPLASRIEVNGKPLSIPGVTQV"
     gene            complement(1265..>2125)
                     /locus_tag="VFY80_00400"
     CDS             complement(1265..>2125)
                     /locus_tag="VFY80_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006386.1"
                     /translation="AIYHPVGIPMLVQNVPNPGAVIGINGLAGNLGIAVAALVTGFLV
                     KWIGWRAAFAIPGLFSIACGIVFALTCPRENEAPARRKSGKAAVVLTPAMLARAFTVM
                     TAAAVASSLLFNFTTNGNSQLLSERFRGLIEDPALLGALLALIYTIASFAQIIVGHLI
                     DRMPFKPLQLWMSIAQVPLLAFAAHTQDWWLFAALLAVMVFIFGSIPFTDAMIVRYVD
                     DRLRSRVSGMRITVAFGVSSLAVWILGPLVKEIGFANALWVLAGIAALKAAIVLLLPD
                     ESTADKVAQA"
BASE COUNT          377 a          647 c          688 g          413 t
ORIGIN      
        1 tcatggcgaa gagcgccgaa ctaggagatc tcacgcttgc ggccaaccag gtgctgcaca
       61 atttcctcgt cttcacttcc ttcgggctcg acggcttcgc acacgcggcg gaggcgatac
      121 tgggcgaggc ggtgggccgg cgcgatcgcg ccgcgttccg acattccatg cgcgtcgttt
      181 tcttctgggc ggcgctggtc ggtgcgctga atctcgtgct gtatgccgtc gcgggccgcg
      241 gcatcatcgc gcttctgacc aatatacctg aggtgcggac cgccgcaggg gagttcctgc
      301 tgtggccggt gattatgccg gtcgtctcgg tgtgggcctt cacctacgat ggcgtgtacc
      361 ttgccgcaac gcgcactacg atcatgcgca ataccgtgat cgcggcattt gttgcattta
      421 tcgtggtgct ccacgtggca acaccgattt acggaaacct gggcgtgtgg atcgccgtga
      481 ccgtttttct cggactgcgg ggcctgctgc tgcatctctt cttttcccgg acgctccgcg
      541 cgttcggcgt ctgattctcc cggaacggtg gagcgtgcca acggtctcaa ggcggccgaa
      601 cgggtgccgg gtccccggga cggcgctcgc gctaggcgct gttcgttgtc ttcccgcgca
      661 ggcggtacga cggtcagacc tgcgtgacgc cggggatgga taagggcttc ccgttcactt
      721 cgatgcggct tgcaagcggc acctgctgcg tgatcaaccc ttcggcgaag catccggcct
      781 ttgcgttctt gacgagatgc gcgagcctct cttcgggctc ctcgctctcg agcttgagat
      841 gagtgctgat tccctcccag cggttaaaaa ccgttccctt gagcaccgag ccgcccagat
      901 gcttgcgcat gaccacgcgg cagcttgctc cggtcacctt catcttcatc atgtgcgcgt
      961 accgcgcaac ttgggtgagc agtcagaatc cgatcgccat cgcgagatag gtcatcggcg
     1021 aaggatagcg gtccgtgccg ccgatgcgcg aaggctcgtc acagtagacc tcgaacttgc
     1081 ccgatgtgcc gcgcttcagc tgcagctcgc cggtcaccat ttcagtcagc acgtcgtttt
     1141 cgttggtaat gccggcagcg ggtagctccg ggcggtcgat cacgcgctct ccgaggcgct
     1201 cggagagatc ttcgtttccc aaaggcttca tggctgtgct cccgtgttga gggccgcaga
     1261 gattctacgc ctgtgcgact ttatcggcgg tcgattcgtc gggaaggagg agcacgatcg
     1321 cggctttcag cgccgcgatg ccggcgagca cccacagcgc gttggcaaaa cctatttcct
     1381 tcaccagcgg ccccagtatc cagacggcga gggaactcac accgaaggcg acggtgatgc
     1441 gcatgcccga gacccgcgag cgcagccggt catcgacgta gcgcacgatc atcgcgtcgg
     1501 taaagggaat ggagccgaag atgaacacca tgactgcgag cagcgcagcg aacagccacc
     1561 agtcctgtgt atgggccgca aaggcgagga gcgggacttg cgcgatggac atccagagct
     1621 ggagcggctt gaagggcatg cgatcgatca gatggccgac gatgatctgt gcgaacgagg
     1681 cgatggtata gatcaacgcg agcagcgcac ccagcaaggc gggatcctcg atgaggccgc
     1741 gaaaacgttc gctcaggagc tgggagttgc cattggtggt gaagttgaaa agcaggctgc
     1801 tcgcgacggc ggctgcggtc atcaccgtga acgcgcgcgc caacatggca ggggtcagga
     1861 cgaccgcggc cttcccgctc ttacgcctgg cgggcgcttc gttctctcgc gggcaagtca
     1921 gcgcgaacac gatcccgcaa gcgatcgaga aaaggccggg aatggcgaac gcggcgcgcc
     1981 agccgatcca tttcacgaga aaaccggtga ccagcgcggc gaccgcgatg ccgagatttc
     2041 cggcaaggcc gttgatgccg atgaccgctc ccggattcgg aacgttctgt accagcatcg
     2101 ggatgccgac gggatggtag atcgc
//
LOCUS       DASURG010000025         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_823967, whole genome shotgun sequence.
ACCESSION   DASURG010000025 DASURG010000000
VERSION     DASURG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823967"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFY80_00405"
     CDS             complement(<1..475)
                     /locus_tag="VFY80_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006387.1"
                     /translation="MNPTGHPSAAKRHVNVLSVVVATMVAQVASIMGVAVFPVIAPRL
                     AAEMGVSPVWVGYQVSLIYGSAMLAAPLLNAAIVRWGGCRATQAGLALNALGLAAALT
                     SNLWALIATSILFGMALAVLVPASAHLLFRFCPPERRNLVFSLKQTGVPIGWTVMA"
     gene            604..1305
                     /locus_tag="VFY80_00410"
     CDS             604..1305
                     /locus_tag="VFY80_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6006388.1"
                     /translation="MGKTITLLTDRAYERIKHDIICCILPPGTEVSEAQLCAQYRLGK
                     APVRMALTRLAHEGLVRAIPRRGYMVTAVTLKDIQDVFELRLMLEPAAARMAAGRVNA
                     QRLRTLDEACRAGYQPGDAKSVSRFIDATKSFHIAIAQAAGNVRLASAVEDLLDEMTR
                     LLHLGLGLRNRSQEMQHEQRALVKALLRGDGATAERLCREQIEATRNMVLSAILTSSS
                     VVHLAITTAGGRAGP"
     gene            1318..2322
                     /locus_tag="VFY80_00415"
     CDS             1318..2322
                     /locus_tag="VFY80_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6006389.1"
                     /translation="MKAVVLREHGGLERLQYVTDFSDPTVTEGHVVIRVRATSFNYHD
                     VFTVHGMPGIKVPMPMIIGLDMAGDIVEVGSGVKAWKPGERVLVNPLNRQKGLMGEMM
                     HGGLAEKCLVAEHQLIRMPDRVTYEQAASLPVAYGTAHRMIVTHDTIRKGDKVLVLGA
                     SGGVGTGCVLLAKMRGAEVIACASSADKLERLKELGADHVINYREADFSKWVIEKFGK
                     PQRRSYDGGVDVVVNFTGGDTWAPTLRCVKRGGKILVCGATAGYDPKEDLRYIWSFEL
                     KVIGSNSFYDENLTALMDLIRRGAMKPVIDVTLPLERAVEGLRMIESREVFGKVVVTP
                     "
BASE COUNT          478 a          793 c          804 g          387 t
ORIGIN      
        1 gcgccatcac cgtccacccg atcggcacgc cggtctgctt gagcgaaaaa accagattgc
       61 gccgctccgg cggacagaat cggaacagca gatgggccga tgccggtacc aacaccgcga
      121 gcgccatgcc gaacaaaatg gacgttgcta tcaacgccca gagattggac gtgagcgccg
      181 cggccaggcc tagcgcgttg agggcgagac ccgcttgtgt cgcgcggcaa ccgccccaac
      241 ggacgatggc ggcattcagc aacggcgcag caagcatcgc cgacccatag atcaggctca
      301 cctgatagcc gacccatacc ggcgagacgc ccatctccgc ggcaaggcgc ggcgcaatga
      361 cgggaaacac ggccacgccc atgatcgatg caacctgcgc caccatggtc gccacgacga
      421 cgctcaatac gttgacgtgc cgcttcgcag ctgacgggtg gccggtcgga ttcacggagg
      481 cgcatacatc ggaagagcgg cattataccg gcgccccggg cgcgtcccgc ctgatatcgc
      541 gttgacatgt cacggagcga ttcctagaat ttcattccct tcacttccgc cgcccgcagg
      601 ttcatgggca agaccataac gctccttacc gatcgcgcct acgagcgcat caagcacgac
      661 atcatctgct gcattctgcc gccgggcacg gaagtttcgg aggcgcagct ttgcgcacag
      721 taccggctcg gcaaggcgcc ggtgcgcatg gcgctcacgc ggctcgccca tgaaggactc
      781 gtgcgcgcga ttccgcggcg cggctacatg gtgaccgccg tgacgctcaa ggacatccag
      841 gacgtgttcg agttgcggct catgctcgag ccggccgccg cacgcatggc ggccgggcgc
      901 gtcaacgcgc agcgcctgcg cacgctcgat gaagcatgcc gcgcgggcta tcagccgggg
      961 gacgcgaaga gcgtttcgcg cttcatcgat gccaccaagt ctttccatat tgcgattgcg
     1021 caagccgccg ggaatgtgcg gctcgcgagc gcggtcgaag acctgctgga cgaaatgacg
     1081 cggctgctcc atctcgggct cgggctgcgc aaccgctcgc aggagatgca gcatgagcag
     1141 cgcgcgctgg tgaaagcgct gctgcgcggc gacggcgcca ccgccgagcg gctctgccgc
     1201 gaacagatcg aagccacgcg caacatggtg ctgtccgcaa tcttgacttc cagctcggtc
     1261 gtccacctcg ccatcacgac ggcgggcggc cgggccggcc cctgacagga gagttgcatg
     1321 aaagcagttg tgctgaggga acacggcggt ctcgagaggc tgcagtacgt gacggacttt
     1381 tccgatccca cggtaacgga agggcacgtc gtcatccgcg tccgcgcgac ctcgttcaac
     1441 tatcacgacg tttttaccgt acacggcatg ccgggaatca aggtgccgat gccgatgatc
     1501 atcggcctcg acatggcggg cgatatcgtc gaagtcggtt ccggcgtcaa agcgtggaaa
     1561 cccggggagc gcgtgctcgt caatccgctc aaccgccaga agggcctgat gggcgagatg
     1621 atgcacggcg ggctggccga aaagtgcctg gtggccgaac accagctgat ccgcatgccc
     1681 gaccgcgtca cgtacgagca ggcagcctcg ctgccggtgg cctacggcac tgcgcatcgc
     1741 atgatcgtta cgcacgacac catccgcaag ggcgacaaag tgctggtgct gggcgcaagc
     1801 ggtggcgtag gtaccggctg cgtgctgctc gccaagatgc ggggcgccga ggtgatcgcg
     1861 tgcgcgagca gcgccgacaa actcgagcgc ctgaaggaac tcggtgcgga ccacgtcatc
     1921 aactacaggg aagcggactt ttcgaagtgg gtgatcgaga agttcggcaa gccgcagcgg
     1981 cgcagctacg acggcggcgt ggacgttgtc gtcaatttca ctggcggcga cacctgggcg
     2041 cccaccctgc gctgcgtcaa gcgtggcggg aagatcctcg tgtgcggcgc gacggccggc
     2101 tacgatccga aggaagacct gcgctatatc tggagcttcg agttgaaggt gatcggctcg
     2161 aacagcttct acgacgagaa cctgaccgcc ctcatggacc tgatccggcg cggcgccatg
     2221 aaacccgtga tcgacgtcac cttgccgctc gagcgcgccg tcgagggtct ccggatgatc
     2281 gagagccgcg aagtgttcgg caaagtcgtg gtgacgccct gagcgatggc gattccgtat
     2341 cccgaaccgc ggggccatcc cggcaacctg gggctcctgt ttgcagacca cgcagaagag
     2401 gcgcgcaccg caatcgtcga tctacgagag ccgggggcac cgctcgagtt gagcttccgg
     2461 ga
//
LOCUS       DASURG010000026         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1334900, whole genome shotgun sequence.
ACCESSION   DASURG010000026 DASURG010000000
VERSION     DASURG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334900"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VFY80_00420"
     CDS             <1..1105
                     /locus_tag="VFY80_00420"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX6006390.1"
                     /translation="RMRPAPAYFSPDYTTARDRFRAAARSAGAEVHVLKLDARGPGGE
                     TLTIDIARLGRPDAQRVLLHTSGLHGVEAFTGSAIQLALLEAPPEPPEKCALILVHVL
                     NPYGMAWLRRANESNVDLNRNFLDECDTWSGAPQIYGRIDAVLNPPSAPGRDFFYLKA
                     LWQTVRHGFGPLKQAVAEGQYEYPRGLFYGGKMLEQGPRLYIAWMTEHLERARYVFGV
                     DVHTGLGRWGTDTLFLEAGAGVTPKEALSAALAHEVVDVAADARAAYRVRGGMGAMLP
                     RVLRKAAVDFVLQELGTYPPLAVFHALREENRWHHWGGGGIDHPVKRLLLERLCPADA
                     VWRSRVLAHGLARARAAAAWAYAGVKPNIPPVE"
     gene            1294..2886
                     /locus_tag="VFY80_00425"
     CDS             1294..2886
                     /locus_tag="VFY80_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006391.1"
                     /translation="MNAQSLNPVGSASGLHFTDSASCKRWVESLPLGNIPQAREILSA
                     ELAALRGTELAAIERLRMLETLKDSVAFVQAEVADRYAGKPLPLDVNEAESWAAVNEL
                     WRHAHSNYERCLQAYRDGDLDIAPHAPLVAMRCLSAIAVQMVEHYRVYRQVPESLWRA
                     LNELYAFAEQHGFARTRPHGLGGQTTEASCVETYVETLLVHLANPYALSVRQLAFVES
                     WSRKWATLVGLSPQPLPSGSSTPLAVSLDTSAGAVLVSEMTARSNVRYLDLEQLSKTL
                     RQTITLLKQGRTPAELGLGEAARQPGCENLLMLLYVQWCRAGTARGEDREATDERVSV
                     CFGITAAHFQVSGRRELRQPTELTAREKQDFDTFGFVPRAPEHRSAEALEVWNILNYS
                     PSGFMCILRDPTSKVRIVHNQLVAVQRRGTRHFQVGMVQWLRVEREREIQLGVRVFPG
                     IPQAVAVRPSNFTPESSRYEAALLLPEVPASATPATLILPPGWFQSGRFVETQSDHKQ
                     VAKLLNLLEVGSDFDRCTITLI"
     gene            2962..3333
                     /locus_tag="VFY80_00430"
     CDS             2962..3333
                     /locus_tag="VFY80_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX6006392.1"
                     /translation="MAVTVQPTEIKLHQKSRILEITFADGRNFRLPCEFLRVYSPSAE
                     VRGHGPGQEVLQAGKKEVTIEKIEPVGHYAVQLTFSDGHDTGIYSWDLLYDYGLQLDE
                     MWARYLKRLADAGASREPQPK"
     gene            3342..>3965
                     /locus_tag="VFY80_00435"
     CDS             3342..>3965
                     /locus_tag="VFY80_00435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6006393.1"
                     /translation="MSSDPRVTDFGYQTVPEEEKAERVAGVFDSVATRYDLMNDLMSA
                     GLHRWWKRFAVEQSAVRPGDRVLDVAGGTGDLARLFARRVGIQGRVVMTDINGSMLRI
                     GRNRLLDEGVIAPIVQCDAEKLPFAADRFDCVSVAFGLRNMTRKPLALAEMRRVLKPG
                     GRLLVLEFSRVWKPLGPLYDAYSFQVLPRLGELIARDAASYRYLAESI"
BASE COUNT          684 a         1272 c         1300 g          709 t
ORIGIN      
        1 gcgcatgcgg cccgcccccg cttatttctc tcccgactac acgacggcac gagaccgctt
       61 ccgggccgcc gcgcgctcag ccggcgctga agtgcacgtg ctcaagctcg atgcgcgtgg
      121 acccggcggc gaaacgctca ccatagacat cgcccgactc gggcgcccgg atgcgcaacg
      181 cgtgctgctc catacgagcg ggctccacgg ggtggaagcc ttcaccgggt cggcaattca
      241 gcttgcgctg ctcgaggcgc cccccgaacc cccggagaaa tgtgcgctca ttctcgttca
      301 cgtgctcaat ccgtacggca tggcctggct gcgacgcgcc aacgagagca atgtggacct
      361 caatcgaaat tttctggacg agtgcgacac gtggtcgggc gccccgcaga tctacggtcg
      421 catcgacgcg gtgctcaatc cgcccagcgc accgggccgg gatttcttct atctgaaagc
      481 gttgtggcag acggtgcgcc acggctttgg tccgctcaaa caggcggtcg ccgaagggca
      541 gtacgaatac ccgcgcggcc tcttctatgg cggcaagatg ctcgagcagg gcccgcgcct
      601 ctacatcgcc tggatgaccg agcatctgga acgggcgcgc tacgttttcg gcgtcgacgt
      661 ccataccggt ctcggccgat ggggaaccga tacgcttttc ctcgaggcgg gcgcgggcgt
      721 aaccccgaag gaggcgctga gcgccgcgct tgctcatgag gtggtcgatg tcgcagcgga
      781 cgctcgcgcg gcataccgcg ttcggggcgg aatgggcgca atgctgccgc gcgttctgag
      841 gaaggcggcg gtggacttcg tgctgcagga actcgggacg tatccgccgc ttgcggtgtt
      901 ccacgccttg cgcgaggaga atcgctggca ccactggggc ggagggggga tcgatcaccc
      961 ggtcaagcgg cttctactcg agcggctttg ccccgcggat gccgtgtggc ggtcgcgggt
     1021 gctcgcacac ggtctggcac gcgcgcgtgc cgccgccgca tgggcgtatg ccggcgttaa
     1081 gccaaatatc ccgccggtcg agtgaacgga aatttatcgc tccggggcct tgcggcagtg
     1141 tcgcgaagtg cgaaaatgtg aactgcttcc gctgcttccg gcatcaattt gagacatggg
     1201 gtgactttgt tcacaggcgg gaaaggcagc gttcagtaag ctattgcgac gggatttggg
     1261 gcgcgaccaa gcgcgcgagg gaggaaggac ggcatgaacg cacaatcgct caatcccgtg
     1321 ggaagcgcat ccgggctgca tttcaccgac agcgcgagct gcaagcgctg ggttgagtcg
     1381 ctccctcttg gcaacatccc gcaggcacgc gagatcctca gcgcagaact tgcggccctt
     1441 cgtgggaccg agctcgcagc gatcgagcgt ctgcgcatgc tcgagacact caaagattcc
     1501 gtcgcttttg ttcaagccga agtcgccgac cgctacgccg gcaaaccgct gccgctcgat
     1561 gtaaacgaag cggaaagctg ggcggccgtc aatgaactgt ggcggcacgc gcattcgaac
     1621 tacgagcgct gcctgcaggc ttatcgcgac ggtgatctcg acattgcccc tcacgccccg
     1681 cttgtcgcga tgcgctgcct gtccgcgatc gcggtgcaga tggtagagca ctaccgcgtc
     1741 taccgtcagg tgccggaatc gctatggcgc gcgctgaacg agctttatgc tttcgccgag
     1801 cagcacgggt tcgctcgaac ccggccacac ggcctgggcg gtcagacgac cgaggcgagc
     1861 tgcgtcgaga cctacgtcga aacgctgctg gtccacctcg ccaatcccta cgcgctgtcg
     1921 gtgcggcagc ttgcattcgt agagtcgtgg tcgcggaagt gggcgacgct cgtgggcctc
     1981 tcgccccagc ccttgccgtc aggaagcagc acgccgctcg cggtcagcct tgacacgtcg
     2041 gccggtgccg tcctcgtgag cgagatgacc gcccggtcga acgtgcgcta tctcgatctc
     2101 gagcagctgt cgaagacgct gcgccagacg atcacgctac tcaagcaggg acggacaccg
     2161 gcggaactgg gactgggcga ggcggcccgg cagcccggat gcgaaaatct cctcatgctg
     2221 ctctacgtgc agtggtgtcg ggccggaacc gcgcgcggcg aagatcgcga ggctacggac
     2281 gagcgcgtta gcgtgtgctt cggcatcacc gccgcccatt ttcaggtgag cgggcggcgc
     2341 gaattacgcc agcccaccga actcaccgcc cgggagaaac aggatttcga tactttcggc
     2401 tttgtgccac gcgcacccga gcaccgaagc gcggaggcgc tcgaagtctg gaacattttg
     2461 aattacagcc cgagcggctt catgtgcatc ctgcgcgatc cgaccagcaa ggtgcgcatc
     2521 gtgcacaacc aactcgtcgc ggtgcagcgg cgcgggacgc gtcattttca ggtcggcatg
     2581 gtgcagtggc tgcgggtcga gcgcgagcgc gagatccagc tcggcgtgcg cgtgttcccc
     2641 ggcatcccgc aggcggtcgc cgtgcggccg tccaatttca ccccggaatc cagccgttac
     2701 gaggcggcgt tgctcctgcc cgaagtgccg gcctcggcga cgcccgccac gctcatcctg
     2761 ccacccggct ggttccagag cggccgcttc gtcgaaacgc agtccgatca taaacaggtc
     2821 gcaaagctcc tcaatctgct cgaagtgggc agcgatttcg accgctgcac catcaccctg
     2881 atctaggcgc agagcgctgt cgctgttctg cggacgcggc gcgtcggtta tgatgcggat
     2941 tcctcgcatc gcgctacgcc gatggccgtc accgttcaac ccacagaaat caagctgcac
     3001 cagaagtcgc gcatactcga aatcacgttc gccgacggcc gcaactttcg gctgccctgc
     3061 gagttcctgc gcgtctactc gccgtcggcc gaggtgcggg gccatgggcc cggccaggag
     3121 gtgctgcagg ccggcaagaa agaggtgacg atcgagaaga tcgagccggt cggtcattac
     3181 gcggtgcagc tgacgttctc cgatggtcac gataccggca tctattcgtg ggacctgctc
     3241 tacgactacg ggcttcaact ggatgagatg tgggcgcgct atttgaagcg tttggccgac
     3301 gcgggcgcga gccgcgagcc gcagccgaag tagagcgcac gatgtcgagc gacccgcgcg
     3361 tgaccgactt cggttatcag accgttcccg aggaggaaaa ggccgaacgc gttgccggcg
     3421 tgttcgattc ggtcgccacg cgctacgacc tcatgaacga tctcatgtcg gcgggcttgc
     3481 accgctggtg gaagcgcttt gccgtcgagc agagcgccgt gcgcccggga gatcgcgtgc
     3541 tagacgtggc gggcggaacg ggtgaccttg cgcggctctt tgcacgacgc gtcggcattc
     3601 agggcagggt ggtcatgacc gacatcaacg gctcgatgct tagaatcggt cgcaatcgtc
     3661 tgctggacga aggcgtgatc gcgccgatcg ttcaatgcga tgccgagaag cttccgttcg
     3721 ccgccgaccg cttcgattgc gtcagcgtcg cctttggtct caggaacatg acacgcaagc
     3781 ccttggcgct cgcggaaatg cgtcgcgtgc tgaagccggg cgggcggctc ctcgtgctcg
     3841 agttctcgcg cgtgtggaag ccgctcggcc cgctttacga cgcctactcg ttccaggtgc
     3901 tcccgcggct gggcgagctt atcgcccgcg acgctgccag ctaccgttac cttgccgaat
     3961 cgata
//
LOCUS       DASURG010000027         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_415434, whole genome shotgun sequence.
ACCESSION   DASURG010000027 DASURG010000000
VERSION     DASURG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415434"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFY80_00440"
     CDS             <1..485
                     /locus_tag="VFY80_00440"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6006394.1"
                     /translation="ADAIRAARVAHHFIGVTKTGACAIVATSGNDDCHIVLRGGRAPN
                     YDAASVEATCSLLSASALTPNVMIDLSHANSSKQYEKQLEAGADVARQVAGGDKRIVG
                     VMAESHLKPGRQDLAPGKPLVYGQSITDACIGWGDSMRLLELLADAVAQRRVAGNPRS
                     "
     gene            complement(563..1822)
                     /locus_tag="VFY80_00445"
     CDS             complement(563..1822)
                     /locus_tag="VFY80_00445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006395.1"
                     /translation="MERATQSAAPTGRRLLAMVFLPFAAGYYVSFLFRNVNSVVFPEL
                     TREFGLSAGSLGLLTSAYFLTFAGAQLPLGILMDRYGPRRVNAAMLLVAALGAVVFAF
                     AGGLPMLTIGRALIGLGVAVCLMSSMTAFVLWFPLERMATLVGWMLLVGAIGALSATK
                     PVELALRVMDWRALFLMLAGYAVCASIAIFLAVPEKKPDGMAPALRSQIADVWTILRD
                     RGFWSIGCAAAFVQGVAISLLGLWAGPWMRDVAGLDRATLAFNLLFAAAAFGVGGVLW
                     GTLSDKLAARGVPVLVTYLIGCAACTAAMIPIVVGYGAASPLLWTLFMALSPFGTLSY
                     PLLAARFPKAMTGRVVTALNMVTFMLAFVVQAGVGAIINLWPAVDGRYAFEGYRTAFG
                     LCFLLQTASVVWLAYAERRNFRSTGTR"
BASE COUNT          423 a          667 c          653 g          274 t
ORIGIN      
        1 cggccgatgc gatacgggcc gcccgcgtcg cccatcactt catcggcgta accaaaaccg
       61 gtgcctgcgc gatcgtcgcg acctccggca acgacgattg ccacatcgtg ctgcgcggcg
      121 gccgggcgcc gaattacgat gccgcgagcg tcgaagcgac atgcagcctg ctcagcgcct
      181 cggcgctcac gccgaacgtg atgatcgatc tgagccatgc caattcctcg aagcagtacg
      241 agaagcagct cgaggcgggc gcggatgtcg ctcggcaggt tgcgggcggg gacaagcgca
      301 tcgtcggcgt gatggccgag agccacctca agccgggccg gcaggatctc gcaccaggca
      361 agccgctcgt atacggacaa agcatcacgg atgcctgcat cggctggggg gacagcatgc
      421 gcctgctcga gctgctggcc gatgccgtcg cgcaacggcg cgtcgcggga aacccgcgct
      481 cctgagtcat tccagcgcca actgcaaatt tttctggatg accggctttc gccggtataa
      541 cgcgcttgca gccctgcgac gcttatctcg tgcctgtgct gcggaaattg cggcgctcgg
      601 cgtaggcgag ccagaccacc gacgcggtct gcagcagaaa gcacagtccg aaggcggtgc
      661 ggtagccttc gaaagcgtag cggccgtcca ctgcgggcca gagattgatg atcgcaccca
      721 cgcctgcctg gacgacgaac gcgagcatga aggtcaccat attgagggcc gtgaccacgc
      781 gcccggtcat cgctttggga aaacgcgcgg cgagcagcgg ataagacagg gtcccgaacg
      841 gtgagagggc catgaagagc gtccagagaa gcgggctcgc agcgccgtat ccgacaacga
      901 tcggaatcat cgctgcggtg cacgccgcgc acccgatgag gtaagtgacg agcaccggca
      961 cgccgcgcgc agcgagcttg tccgagagcg tgccccacag cacgccgccc acgccgaacg
     1021 cggccgccgc aaaaaggaga ttgaaggcaa gcgtcgcgcg gtcgaggccc gcgacgtcgc
     1081 gcatccacgg tcccgcccag agcccgagaa gactgatcgc aacgccctgg acgaaggccg
     1141 ccgcgcagcc gatcgaccag aagccgcgat cacgcagaat cgtccataca tccgcgatct
     1201 ggctgcgcag cgccggcgcc atgccgtccg gctttttctc cggaacggcg agaaagatcg
     1261 cgatgctcgc gcacacggca tagccggcca gcatgagaaa caacgcacgc caatccatga
     1321 cgcgcagggc aagctccacc ggcttcgtgg cggaaagcgc accgatcgcc ccgacgagca
     1381 gcatccatcc gacgagcgtc gccatgcgct ccagcggaaa ccacagcacg aaggccgtca
     1441 tggacgacat gaggcacacc gcgacaccga gcccgatcag ggcccgcccg atggtcaaca
     1501 tgggaagccc gcctgcgaag gcgaagacga ccgcgcccag cgcggcgacg agcagcatgg
     1561 ctgcattcac ccgccgcggg ccatagcggt ccatgaggat accgagcgga agctgcgcgc
     1621 ccgcaaaggt gagaaagtat gcgctcgtga gtaaacccag cgacccggct gacaagccga
     1681 attcgcgcgt cagttcggga aagacaaccg agttgacgtt gcggaacaga aaggagacgt
     1741 agtagccggc cgcgaacggc aggaacacca tggcgagcag ccggcggccg gtcggcgccg
     1801 cggactgcgt cgcgcgttcc atgtatcgtc cccgcaccgg cttcccgtcc gtagatcgcg
     1861 gcggtagtgt agccgaacgc cgcaaggtcc tgcgccagcg atgagctgat cgtccgttga
     1921 cagtgggata tcaggctcct accatcgttt gcccctgatt cgtcgtgctc gaggagaaaa
     1981 tacatgcatg cccaaagggc gacactcatc gttctgg
//
LOCUS       DASURG010000028         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1335600, whole genome shotgun sequence.
ACCESSION   DASURG010000028 DASURG010000000
VERSION     DASURG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335600"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /gene="hemN"
                     /locus_tag="VFY80_00450"
     CDS             complement(<1..1015)
                     /gene="hemN"
                     /locus_tag="VFY80_00450"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX6006396.1"
                     /translation="MNSSIAHLPLDVDLDLIRRLDCSGPRYTSYPTADRFVEAFGPES
                     YRSWAGKRNIGGIQRALSLYVHLPFCSTLCFYCACNKIATRDHAKGDKYLGYLAREIE
                     LQAELFRRDPLVRQMHWGGGTPTFFAAEQLEALFARLRGAFRLADDGEYSIEIDPRTV
                     DARYIAALRAMGFNRMSLGVQDFDPEVQRAVNRIQSPEQTLGVMSAARDAGFLSVNVD
                     LIYGLPRQNLISFNRTLARIIAAQPDRIAIYNYAHLPARFKPQRRIKESDLPAPEVKL
                     KLLGLAARRLAEAGYVYIGMDHFAKPGDALAVAQRQGRLHRNFQGYSTQPDCDLIGLG
                     VSSI"
     gene            1119..1862
                     /gene="fnr"
                     /locus_tag="VFY80_00455"
     CDS             1119..1862
                     /gene="fnr"
                     /locus_tag="VFY80_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEX6006397.1"
                     /translation="MTPHSGLSSVDVNRLKAACSTCSLRELCLPAGLDTRELERVDRM
                     VNRRRPVRRGDYLYRSGTQLESLYAIRTGFMKSCVLHDDGREQVAGFHMMGDLMGLDA
                     ISTGRHMCDVVALEDTEVCDIPLARLEQLSRDIPSLQQHFHRIMSREIVRDHGVMLLL
                     GTMRAEERLAAFLLNLSQRFAARGYSSNEFYLRMTREEIGSYLGLKLETISRALSRFH
                     EEQLIEVHTKYVRILDMDTLRRMVGVPVS"
     gene            complement(1870..2151)
                     /locus_tag="VFY80_00460"
     CDS             complement(1870..2151)
                     /locus_tag="VFY80_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX6006398.1"
                     /translation="MGAARVVAGMLLVAVLPAEGADIPSVERGRLLYENHCVVCHTPN
                     IHRRPNRVPLNAAELRDIVNGWQREEKLRWSEQDVADVVQYLRETRYKF"
BASE COUNT          388 a          742 c          724 g          395 t
ORIGIN      
        1 cgatcgacga tacgccgagc ccgatgagat cgcagtcggg ttgcgtcgaa tagccctgga
       61 agttgcgatg caggcgcccc tggcgctgcg cgacggcgag cgcatcgccg ggcttcgcga
      121 agtgatccat gccgatgtag acgtagcccg cttcggcgag ccggcgcgcc gcgaggccca
      181 gcagcttgag cttgacttcc ggcgcgggca gatcgctttc cttgatccgc cgctgcggct
      241 tgaagcgcgc cggaaggtgc gcatagttgt agatcgcgat gcgatccggc tgggctgcga
      301 tgatacgggc gagcgtgcgg ttgaagctga tgagattctg acgcggcagt ccatagatca
      361 gatcgacgtt caccgagaga aagccggcgt cgcgcgccgc cgacatcacc ccgagcgtct
      421 gctccgggct ctgaatgcgg ttcaccgcgc gctgcacctc cgggtcgaag tcctgcacgc
      481 ccaggctcat gcggttgaaa cccatcgcgc gcaacgccgc gatgtaacgg gcgtccaccg
      541 tgcgcggatc gatctcgatc gaatactcgc cgtcgtccgc aagcctgaat gcgccccgca
      601 ggcgggcgaa aagtgcctcg agctgctcgg ccgcgaagaa cgtcggcgtg ccgccgcccc
      661 agtgcatctg acgcacgagc ggatcgcgcc tgaaaagctc cgcctgcagc tcgatctcgc
      721 gcgcgagata gccgaggtac ttgtcgccct ttgcgtggtc gcgcgttgcg atcttgttgc
      781 acgcgcagta gaagcagagc gtgctgcaga acgggaggtg aacgtagagc gagagcgcgc
      841 gctggatgcc gccgatgttg cgcttgcccg cccacgaccg atagctctcc ggcccgaacg
      901 cctcgacgaa acggtccgcg gtggggtacg aggtatagcg cggaccgctg cagtccagcc
      961 gccggatcag gtcgaggtcc acgtccagcg gcaggtgcgc gatcgatgaa ttcatggatt
     1021 gtgctcgctc cttgggcgcc agtattgcac ccgccggcgg gaacgacttg atccacatca
     1081 aacgccgtgt aacatcgcga tatatcctac gcaatgtcat gacaccccat tccggcctgt
     1141 cctccgtgga cgtcaatcgc ctcaaggcgg cgtgctcgac gtgcagtctg cgcgagctgt
     1201 gcctgccggc cggtctcgac acgcgcgagc tcgaacgcgt ggaccggatg gtgaaccggc
     1261 gccggccggt gcggcgggga gactacctct accgctccgg cacgcagctc gagtcgctct
     1321 atgcgatccg caccggcttc atgaagagct gcgtgctgca cgacgacggc cgcgagcagg
     1381 tcgcgggctt tcacatgatg ggagacctga tggggctcga cgccatcagc accgggcggc
     1441 acatgtgcga tgtggtggcg ctcgaggaca ccgaagtgtg cgacatcccg ctcgcgcggc
     1501 tggagcagct ttcgcgcgac atcccgtcgc tccagcagca ctttcaccgc atcatgagcc
     1561 gcgagatcgt gcgcgaccac ggcgtgatgc ttctgctcgg gacgatgcgc gctgaagagc
     1621 ggctcgccgc cttcctcctc aatctctcac agcgcttcgc ggcgcgcggg tactcctcga
     1681 acgagtttta tctgcgcatg acgcgcgagg agatcggaag ctacctggga ctcaagctcg
     1741 agacgatcag ccgtgcgctg tcgcgcttcc acgaagagca gctgatcgag gtgcatacca
     1801 agtacgtcag gatactcgac atggacacgc tgcgccgcat ggtcggcgtg ccggtctctt
     1861 gaacgggtac tagaacttgt agcgggtttc ccgcagatac tgcaccacgt cggccacgtc
     1921 ctgctcgctc cagcgcagtt tttcttcgcg ctgccagcca ttcacgatgt cgcgcaattc
     1981 cgcagcgttc aggggcacgc ggttgggccg ccgatggatg ttcggcgtgt gacacaccac
     2041 gcagtggttc tcgtaaagca ggcgaccgcg ctcgacactc gggatgtcgg cgccctccgc
     2101 cggtaaaacg gccacgagca gcatcccggc aaccacgcgc gccgcgccca tcaacggttg
     2161 cgaccgcgcc gcagcactgc ttcgatttcg gtcttggcct cctgccgatc ctcttcccgg
     2221 cagccctcgc cttcgacctg acgaaagcg
//
LOCUS       DASURG010000029         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1131121, whole genome shotgun sequence.
ACCESSION   DASURG010000029 DASURG010000000
VERSION     DASURG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131121"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1595
                     /locus_tag="VFY80_00465"
     CDS             228..1595
                     /locus_tag="VFY80_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6006399.1"
                     /translation="MTSRSTPPYLETLSRFICSTTLADLSAEAKERARWVIADCVPVI
                     AAGMQQPEMKAFVARHLERAAPGSAWVIGAKRRAAALDAALLNGTAGTWLELDEGNLF
                     AKGHPGIQVVPAAIALAQEERASGADLLLAVALGYEASARVSRASQMRLTIHPHGTYG
                     VMGAAIAAGRLKKFNADQMRELLNVAATMGMATSRQTLLDGATVRNIFTGHSGYMGLT
                     AARLVECGFTGEIDGIANIYGRVLSDTFDRDAVISGLGTEWLITRNYFKLHPTGRYVH
                     SAIDALENLLEHVPGGRLNVERIKEIKVRTYMLAAMLAEKKVVSSFGARFSIPFALAS
                     ILYHGRSGLASFDEQAVANPKIQALASRVDLKEDERHTARYPDEQRCELTVTLDDGTT
                     FEGRCTVMKGEPANPHRPEELEGKFSELGTPLWGRIVTRNLFEGLRHLETIPDFGAFA
                     SEFSL"
     gene            1614..2504
                     /locus_tag="VFY80_00470"
     CDS             1614..2504
                     /locus_tag="VFY80_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6006400.1"
                     /translation="MLKTHGRYDYSPITKRADYSWPGGKRLAVHFSLNVEHFAFGEGL
                     GNDYGAPHPPPNSRSYAWRDFGNRVGAWRLIELADRYEIPYAVLLNTELYDYCPELIA
                     AFRARGDEIVGHGRTDAERQADMSYEEERACIFEATAAIEKHEGKKPSGWLAPYISQT
                     YDSLDLLKEAGYRYMMDWPLDDQPVWFRTKHGPILSVPYAHDLNDSIECVSRRTAAQL
                     HCENLIDHFDALLEESERRPLVMPIVLHSFVLGQAYRARQFGRVVEHILRSRDRIWIT
                     RPGDICAYIESLPKGIVPGS"
BASE COUNT          488 a          844 c          833 g          473 t
ORIGIN      
        1 ggattccgat ccgccgctta cgcagtgccg tcttcccggc gtacgccggc atccagagag
       61 gcgcctggat tccggctttc gccggaatga cgagcgggcg ggagtgacga ggtggtcgct
      121 ttcgccggaa tgacgggtcc tccggagcgg gtgcttataa tgagcacgtc ccgcaccaag
      181 tgcgtcacgc ccgaatctca caatcacctc caacggccat tgagcatatg acttcgcgca
      241 gcacgccgcc ctatctcgaa acgctgagcc gctttatctg cagcacgacg ctcgccgatc
      301 tttccgcgga agcgaaggag cgcgcgcgct gggtcatcgc cgactgcgtt cccgtcatcg
      361 ccgccggcat gcagcagccg gagatgaaag cattcgtcgc gcgccatctc gagcgtgccg
      421 cgcccggcag cgcatgggtg atcggcgcaa aacggcgtgc cgccgcgctc gatgccgcgc
      481 tcttaaatgg caccgcgggc acgtggctcg agctcgacga aggcaatctc ttcgccaagg
      541 gccatccggg catccaggtg gtgccggcgg cgatcgcgct cgcgcaggaa gagcgcgctt
      601 caggcgccga tctgctgctc gccgtcgcgc tgggttacga agcgtcggca cgcgtgagcc
      661 gcgcgtcgca gatgcgcctt acgattcatc cccacggcac ctacggcgtg atgggcgccg
      721 caatcgcagc ggggcggctc aagaagttca acgccgatca gatgcgcgag cttctcaacg
      781 tcgcggcgac catgggcatg gcgacgagcc gccagacgct cctcgacggt gcaaccgtgc
      841 gcaacatctt taccgggcat tcgggataca tggggctgac cgcagcgcgc ctcgtcgagt
      901 gcggcttcac cggtgagatc gatggcatag cgaacattta cggcagggtt ctctccgata
      961 ccttcgatcg ggacgccgtg atatcgggat tgggcacgga gtggctgatc acgcgcaact
     1021 atttcaagct ccatcccacc gggcgctacg tgcattccgc catcgacgcg ctcgagaacc
     1081 tgctggagca tgtcccgggc ggccggctca acgtagagcg catcaaggaa atcaaggtgc
     1141 gcacttatat gctcgcggcg atgctcgctg aaaagaaggt tgtctcgagt ttcggcgcgc
     1201 gcttttctat tccgttcgcg ctcgcgagca tcctctatca cgggcgctcc gggctcgcga
     1261 gtttcgacga gcaggcggtc gccaatccga aaatccaggc actcgcctca cgcgtcgatc
     1321 tgaaggaaga cgagcggcac acggcgcggt atccggacga gcagcgctgt gagctcaccg
     1381 tcacgctcga cgacgggacg actttcgaag gccgctgcac cgtgatgaaa ggcgagcccg
     1441 cgaatcccca tcggcccgag gaactcgagg gcaagttttc cgaactcggt acgccgttgt
     1501 ggggcaggat cgtcacgagg aatctgttcg aggggctcag gcacctcgag accattccgg
     1561 acttcggtgc tttcgcgtcc gaattctccc tttagatagc gtcgagtcga gccatgttga
     1621 agacccacgg tcgttacgat tattcgccga tcacgaagcg ggccgactac agttggccgg
     1681 gcggaaaacg cctcgcggtg cacttttcgc tgaacgtcga gcatttcgca ttcggcgagg
     1741 gcctgggcaa cgattacggc gcgccacatc cgccacccaa ttcgcgcagt tacgcgtggc
     1801 gcgatttcgg caaccgcgtg ggcgcgtggc gtctcatcga actggcggac cgctacgaga
     1861 ttccctacgc cgtccttctc aacaccgagc tctacgacta ctgcccggag ctgatagcgg
     1921 cgttccgcgc gcgcggcgac gaaatcgtcg gccacggccg gacggatgcc gaacgccagg
     1981 ccgacatgag ctacgaggaa gagcgcgcct gcatctttga agccaccgcg gcgatcgaaa
     2041 agcacgaagg taagaagccg tcggggtggc tcgcgccgta catctcgcag acctacgact
     2101 cgctcgatct cctgaaggaa gcgggctacc gctacatgat ggactggccg ctcgacgatc
     2161 agccggtgtg gttcaggacc aagcacgggc ccattctctc cgtgccctac gcgcacgacc
     2221 tcaacgattc gatcgaatgc gtgtcgcgcc gtacagccgc gcagcttcac tgcgaaaacc
     2281 tgatcgatca cttcgatgcg ctgctggaag agtcggagcg gcgcccgctc gtaatgccga
     2341 tcgtactcca cagcttcgtg ctcgggcagg cgtatcgcgc gcggcagttc ggccgcgtgg
     2401 tggagcatat cctgcgcagc cgcgaccgca tctggatcac gcggcccggc gacatctgcg
     2461 cctatatcga gtcgctgccg aaggggattg tgcccggaag ctgaggtgcg ctgtcgagga
     2521 ggagaggcaa tgaataacga atcacagcag cttgcgtttc tctttcgagg tgtggcgctc
     2581 gccgtgcttc tttttatgtg cggcccgacg atcgccgcgc agagtgcgag ctacccga
//
LOCUS       DASURG010000030         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1131569, whole genome shotgun sequence.
ACCESSION   DASURG010000030 DASURG010000000
VERSION     DASURG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131569"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFY80_00475"
     CDS             complement(<1..662)
                     /locus_tag="VFY80_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6006401.1"
                     /translation="MNPRDFQLDAYWMPFTANRQFKAAPRLFVAAKDMHYVTEDGRRV
                     LDGTSGLWCVNAGHCRPRIVEAIKKQAETMDYSPAFQMGHPGAFRVAERVAKLAPGDL
                     DHVFYANSGSEAVDTALKIALAYHRARGDAQRTVLIGRERGYHGAGFGGMAVGGIPAN
                     RKAFGNTLGRVDHLGHTHCPQENAFSRGQPAWGAHLADELENRIIPLHDGSNIAAVIV
                     EP"
     gene            761..1600
                     /locus_tag="VFY80_00480"
     CDS             761..1600
                     /locus_tag="VFY80_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX6006402.1"
                     /translation="MARHIACLTFDFDALSGWIARGMTTPGPISRGEFGPNVALERIL
                     ALLARCGVPSTWFIPGHTLETYPRACERVLAAGHEIGHHGWTHRPPASLSREEEEAEL
                     VRANDAIRKLTGRTARGYRSPSWDLSTHSVALLLEHGFIYDSSMMGDDYTPYRVREPD
                     VIELERPAVFGKPTRLIEMPVSWTLDDYPHFEFIRTPTVVLPGLMNAQSVLGNWIDDF
                     IYLRDHLDWGVITYTFHPFVIGRGHRMVALEKLVRTLADDGAVFLTMEQAVREFVAHE
                     SAS"
     gene            1655..2398
                     /locus_tag="VFY80_00485"
     CDS             1655..2398
                     /locus_tag="VFY80_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6006403.1"
                     /translation="MIALENVSRSYGPHVALAPTSIAFSAHATTALIGPSGCGKSTLL
                     RLLVGLIQPTTGRILIQGEPLAAENVKRVRHWIGYVIQDGGLFPHLTARHNVTLLARH
                     LKRDARWIAERTEALAELMRLDQSTLDRFPRDLSGGQRQRVSLMRALMLDPPILLLDE
                     PLGALDPITRYELQDELERLFSGLRKTVILVTHDMTEAAHLSDRIAVMRAGRILQHGT
                     LAELLHQPAEDYVTDFIRAQRRSLPGISA"
     gene            2395..>2736
                     /locus_tag="VFY80_00490"
     CDS             2395..>2736
                     /locus_tag="VFY80_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6006404.1"
                     /translation="MRRMLVICALIAPTLCAAQESIGVGSKRFTESYILGEIAAQTVR
                     SARETNVVHHRGLGNTAILFAALKSGAIDVYPEYTGTIAIELLREPGGASLTEINSKL
                     ASHGLAAAIRLG"
BASE COUNT          475 a          876 c          880 g          505 t
ORIGIN      
        1 atcggctcga cgatgacggc tgcgatattg gacccgtcgt gcagcgggat gatgcggttt
       61 tcgagctcgt cggcgaggtg cgcaccccac gccggttgtc cgcgactgaa tgcgttctcc
      121 tgcggacaat gcgtgtgtcc caagtgatcg acgcggccga gggtgttgcc gaacgccttg
      181 cggtttgcgg ggatgccgcc gaccgccatg ccgccgaagc cggcgccgtg gtagccgcgc
      241 tcgcggccga tcagtacggt gcgctgcgcg tcaccccgcg cgcgatgata ggcgagcgcg
      301 atcttgagcg cggtatccac ggcttcggac cccgaattcg catagaacac gtggtcgaga
      361 tcccccgggg cgagcttcgc gacgcgttcg gcgacgcgga acgcgccggg gtggcccatc
      421 tgaaacgccg ggctgtaatc catggtctcc gcctgcttct tgatcgcctc gacgatgcgc
      481 ggacggcaat gcccggcatt gacgcaccac agcccggagg tgccatcgag gacacgccta
      541 ccgtcttcgg tcacgtaatg catgtccttt gcggcaacga agaggcgggg agccgccttg
      601 aactggcggt tcgcggtaaa cggcatccaa taggcgtcga gctgaaagtc ccggggattc
      661 atgtgtgctt tcctcgaggg ggttagcgtg gggtacccca ttctgccagc atggcgcccg
      721 gccgtcatcc ggcagagcac gcgtgctaac atccgggcgc atggcccgac atatcgcctg
      781 cctcacattc gattttgacg ccctgtcggg atggatcgcg cgcgggatga caacgcccgg
      841 gccgatctcg cgcggagaat tcgggccgaa cgtcgctctg gagcgcatct tggcgttgct
      901 tgcgcgctgc ggggtgccga gcacctggtt cattccggga catacgctcg agacgtatcc
      961 ccgggcctgc gagcgcgtgc tggcggccgg acatgaaatc ggccaccacg gctggaccca
     1021 tcgcccgccg gcaagccttt cacgcgagga ggaggaagca gagctcgtgc gcgcgaatga
     1081 tgcgattcgc aagctcaccg ggcgcaccgc gcgcggttat cgctcgcctt catgggatct
     1141 ttccacgcat tcggtggcgc tgctgctcga gcacggtttc atctacgaca gcagcatgat
     1201 gggcgacgat tacacgccgt accgcgtgcg cgagccggac gtgatcgagt tggagcgccc
     1261 agcggtgttc ggcaagccga cccggctgat cgaaatgccc gtcagctgga cgctcgacga
     1321 ctatccgcat ttcgaattca ttcgcacacc gacggtcgtc ctgcccggac tcatgaatgc
     1381 gcaaagcgtg ctcgggaact ggatcgacga cttcatctat ctcagggacc atctcgactg
     1441 gggcgtcatc acgtacacgt ttcatccgtt cgtgatcggc cgtgggcatc gcatggtggc
     1501 gctcgagaag ctcgtccgca cgctggcgga tgacggcgcg gtttttctca cgatggagca
     1561 ggctgtgcgc gagttcgtgg cgcacgagtc cgcgtcctga ggggccgcct ccgcgcgctg
     1621 aagccgccac gttcgatcgc gcggataaag agtcttgatt gctcttgaaa acgtttcccg
     1681 gtcctacggg ccgcacgtcg ccctggctcc gacgagcatc gccttttcag ctcacgcgac
     1741 gacggcgctg atcgggccga gcggttgcgg aaaatcgacg cttttgaggc tgctcgtagg
     1801 actgatacag ccaaccaccg gcaggatcct cattcagggt gagccgcttg ccgcggaaaa
     1861 cgtaaaacgc gtgcggcact ggatcggcta cgtgatccag gacggcgggt tatttcccca
     1921 cctgactgcg cgccacaatg tcacgctgct cgcccgccac ctgaagcggg acgcacgttg
     1981 gattgcggag cggacggaag cgcttgccga gctcatgcga ctggatcagt cgactctcga
     2041 tcgttttccg cgcgatcttt ccggaggaca gcgccagcgc gtgagcctca tgcgcgcgct
     2101 gatgctcgat ccgccgatcc tgctcctcga cgagccgctg ggcgccctcg atccgatcac
     2161 ccggtatgag ttgcaggatg agctggagcg cctcttttcc ggattacgaa agaccgtgat
     2221 cctggtgacg catgacatga ccgaggcagc gcacttgagc gaccgcatcg ccgtgatgcg
     2281 ggccggccgc attctccagc acggcacgct ggcggaactg ctccaccagc cggcggagga
     2341 ctacgtaacc gatttcattc gggcgcagcg tcgcagcctt ccgggaatct cagcatgagg
     2401 cgcatgctcg tcatctgtgc gctcattgcc cccacgctct gcgccgcaca ggaatcaatc
     2461 ggcgtgggct caaagcgctt caccgagtcc tacatcctgg gtgagatcgc tgctcaaacc
     2521 gtgcgcagcg ctcgagagac gaacgtcgtg caccatcgcg gcctcgggaa taccgccatt
     2581 ctgttcgcag cgctgaaaag cggtgcgatc gatgtctacc cggagtacac cggcacgatc
     2641 gcaatcgaac tgctgcgcga gccgggagga gcgagcctga ctgagatcaa cagcaagctc
     2701 gcctctcacg ggctcgcggc ggcaatccgg cttggc
//
LOCUS       DASURG010000031         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_519143, whole genome shotgun sequence.
ACCESSION   DASURG010000031 DASURG010000000
VERSION     DASURG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519143"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1187)
                     /locus_tag="VFY80_00495"
     CDS             complement(198..1187)
                     /locus_tag="VFY80_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX6006405.1"
                     /translation="MAANAKVVLTDYVWESLDVEKKTLAGLADLVALQTKKPDEFITA
                     AADCDALLNTYAGPITAEVMAKMPKCKIIARYGIGVDTIDLDAATAAGIIVTNNPSYC
                     IEEVAEHTMALLLTSARKVAFYDRLVRTGRWEVPPGKPLFRLAGRTLGLVGFGNIARQ
                     VAVRAASFGMRVLFADPYIKDGQFKEPGKKVEMGELLRESDYVSLHPPLTPQTRKMMS
                     DEAFSQMKPTAVLINCARGPVVDTDALVRALDAKKLAGCALDTTDPEPLPNPHPLRGR
                     ENVLLNPHVAWYSEQALVGLQAGAPNEVRRVLTGEWPVNVVNRAVKGRNRAGL"
     gene            complement(1204..2190)
                     /locus_tag="VFY80_00500"
     CDS             complement(1204..2190)
                     /locus_tag="VFY80_00500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX6006406.1"
                     /translation="MTRPRIFVTQPVAESALERLRKVAEVDLNTDPVHIATKDELLDA
                     VRSHEILFCLLHDRVDRDVIAANPGLKAVASMKITPSDIDVEEATRRRIPVTVIPPIV
                     TEATADLNMALMLAVTRRILEGDQLVRKGIFPGAQSSYLEGVMLGGKVLGLIGGGGRI
                     GRAVARRALGFGMKLIYSSPRRKPEAEQELGMKYVELDELLAQADIVSVHTPLTPETR
                     HQLGAREIGLMKPTAYLVNTSRGPIIDEAALTRALLERRIAGAALDVFEHEPKVDPRL
                     ITLSNVVLTPHLGSAARELREVMANVVVDNIMAVLEGHPPPNCWNKEIYSVS"
     gene            complement(2187..2552)
                     /locus_tag="VFY80_00505"
     CDS             complement(2187..2552)
                     /locus_tag="VFY80_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006407.1"
                     /translation="MLGQALGLTDAEFDALQGEYRASSLFTDREKAALAWSEAMTLNT
                     AKRDEAIWAELRHHFNDAEIVEISLACAMFNMINRLNDSFRTELEPEEYNRRQHGAVG
                     VTAQALEDYACRICEAART"
BASE COUNT          436 a          840 c          855 g          525 t
ORIGIN      
        1 gctcaccgcg gtcgatcccg cgctgtggtc cgaggaactc cgggatcacg cgcggctttt
       61 cgagaccctg cagagccgca tgccgcgcga gctcaccctc cagcgcgagc tgatggaatt
      121 gacactgccg cactaggcgg gcgtcaaaat aagacggggc gccgcggcgc cccttttttg
      181 tctgttaccg gaatttgtca gagccccgcg cgattcctgc ccttcacagc gcggttcacc
      241 acgttcaccg gccattctcc ggtaagcacg cgtcttacct cgttcggcgc gcctgcctgc
      301 aggccgacca gtgcctgctc gctgtaccag gcaacgtgcg gattgagaag cacgttctcg
      361 cggccgcgca gagggtgcgg gttcggcagc ggctcggggt cggtggtgtc gagcgcgcaa
      421 ccggccagtt tcttggcatc cagcgcccgc accagcgcgt cggtatcgac caccggaccg
      481 cgggcgcaat tgatgaggac cgcggtcggc ttcatctgcg agaacgcttc gtcgctcatc
      541 atcttcctcg tctgcggggt gagcggggga tggagtgaaa catagtcgga ttcgcgcagc
      601 agttctccca tctctacctt tttccctggc tccttgaact ggccgtcctt gatgtagggg
      661 tcggcgaaga gcacgcgcat gccaaagctt gcggcgcgaa cggcgacctg ccgcgcgatg
      721 ttgccgaaac cgacgaggcc cagcgtgcga cctgccaggc ggaagagcgg cttgcccggg
      781 ggcacttccc agcggcccgt gcgcaccagc cggtcgtaga acgcaacctt gcgggcgctt
      841 gtaaggagga gtgccatggt gtgctcggca acttcctcga tgcagtacga tgggttattg
      901 gtcacgatga tgcccgccgc agtggcggcg tcgaggtcga tggtatctac gccgattccg
      961 taacgtgcga tgatcttgca cttcggcatc ttcgccatca cctcggccgt gatcggcccc
     1021 gcgtaggtgt tgaggagcgc atcgcagtcg gcggccgcgg tgatgaattc atcgggcttc
     1081 ttggtctgca acgcgaccag atcggccagc cctgcgagcg ttttcttttc cacgtccagc
     1141 gattcccaga cgtaatcggt cagcaccact ttggcgttcg cggccatgac gggactcctc
     1201 tctctacgag accgagtaga tttccttgtt ccagcagttg ggcggcgggt ggccttccag
     1261 gaccgccatg atgttgtcca ctacgacatt cgccatcacc tcgcgaagtt cgcgcgcggc
     1321 actgccgaga tgcggcgtca ggacgacgtt ggagagcgtg atgagccgcg gatccacctt
     1381 cggctcgtgc tcgaacacgt cgagtgcggc gcccgcgatt cggcgctcga gcagcgcgcg
     1441 ggtgagggcg gcctcgtcga tgatcgggcc gcgcgaggtg ttgacgagat acgccgtggg
     1501 cttcatgagt ccgatctcgc gcgcccccag ctgatggcgc gtttccggcg tgagcggcgt
     1561 atgaaccgag acgatatcgg cctgcgcgag aagctcatcc agctctacgt atttcatgcc
     1621 gagctcctgt tcggcttccg gcttgcgccg agggctcgag tagatcagct tcatgccgaa
     1681 tccgagtgca cggcgcgcca cggcgcggcc gatgcgcccg ccgccgccga tcaacccgag
     1741 caccttgccc cccagcatga cgccctcgag gtaggaagac tgagcgccgg ggaagatccc
     1801 cttgcgcacg agctgatcgc cttcgagaat acggcgcgtc acggcgagca tgagcgccat
     1861 gttgagatcg gcggttgctt cagtgacgat cggcgggatg acggtgaccg ggatccggcg
     1921 acgcgtcgct tcttccacgt cgatatcgga cggcgtgatc ttcatcgaag cgacggcctt
     1981 gagccccgga ttcgccgcaa tgacgtctcg atccacccgg tcgtgcagca ggcagaacag
     2041 gatttcgtgg ctgcgcaccg catcgagcag ctcgtccttg gtcgcgatgt gcaccggatc
     2101 ggtgttgaga tcgacttcgg cgaccttgcg caggcgctcg agcgcgctct ccgctacggg
     2161 ctgtgtgaca aaaatgcgag gccgcgtcat gtgcgtgcag cctcacagat gcggcaggcg
     2221 tagtcttcga gggcctgcgc ggttacgccg acggccccgt gctgtcggcg gttgtattcc
     2281 tcgggctcga gctcggtgcg gaacgagtcg ttcaaacggt tgatcatgtt gaacatcgcg
     2341 cacgcgagcg agatttccac gatttccgcg tcgttgaagt ggtgccgcag ttcagcccat
     2401 atggcctcat cgcgtttcgc ggtattgagc gtcatcgctt ccgaccaagc gagcgcagcc
     2461 ttttcgcgat cggtaaagag actgctcgcg cggtattccc cctgcagagc atcgaactcc
     2521 gcgtcggtaa ggcccagtgc ttgaccaagc acccggttgt gctccgttca gtatcggcag
     2581 ccgttgagcg tggaggtctt gagaatagcg agattgcgca gcctcacgtc cgacaccgca
     2641 cctgctctag gctgcc
//
LOCUS       DASURG010000032         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_416921, whole genome shotgun sequence.
ACCESSION   DASURG010000032 DASURG010000000
VERSION     DASURG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416921"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFY80_00510"
     CDS             <1..396
                     /locus_tag="VFY80_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006408.1"
                     /translation="QLSGRVPRFFADWCKKNPADPDWNGVSWPAWFFANNWHEVVYYA
                     VAQKCVNPASAGCSAAGAFLTVYGLPPPNDNIRAIVITPGRAYAGQTRPCAAASDCLE
                     DPENTNADDYYRKSSVTPTVNDRLLVVSP"
     gene            393..1169
                     /locus_tag="VFY80_00515"
     CDS             393..1169
                     /locus_tag="VFY80_00515"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6006409.1"
                     /translation="MKFALHKAGFTLIELAVAIFVLMLLLGSLIVPLTTQVEQRKVSE
                     TRKALDEAREALLGFAAANGRLPCPASSASNGNESFAAGGSAADGNCSNFFDGFLPAA
                     ALGLAPTDALGFAVDGWGLVQNRIRYAVSKQTVNGVTNALTRSGGMSAAGLASLAASQ
                     LLYVCGSGIGVNAGTNCGTAVTLSSNAVAIIYSVGANAATGGTSAHELENPNPNGGSA
                     DRIFVSRDWSTASGSEFDDMVTWIGTNPLFSRLIAAGQLP"
     gene            complement(1135..2871)
                     /locus_tag="VFY80_00520"
     CDS             complement(1135..2871)
                     /locus_tag="VFY80_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6006410.1"
                     /translation="MNASALLAQRLITAHPQWSIALMLIALHAALAWGIHAWWVPGAL
                     LAHFGLFLLWQPVWRGEQEIDRRHAILVVVAGTVLAAWNSWWLIAVWLAVLFGLIGGS
                     VPGTADRRQRRASLLAAFYVLSLLLIWAVPHLFSDEVPDAMHVLLVRFGLFFVPVAIL
                     LTRFESRPAEGPVAVDLFYSVLLFLLVMVVVLGSFVVKVLSQGIYLLALAQTLFVMAF
                     LLVALSWLWNPHSGFAGLGHMLSRYVMSLGLPFERWVQQLASYAEQESEPQRFLAQAL
                     QHMLTLPWVGGVRWETQSGRGEFGEPSEHTSVFVIRDLTLKVSTRWALTPAVLLHLRL
                     LAQMVGHFYDAKRRELIQRQTAYTQAVYETGARLTHDVKNLLQSLRSLCAAAEASDAG
                     EAAALQALMQRQLPQITQRLNATLDKLRTPHPAEADVVEAAIWWEGLKQRFATRGVKF
                     VPETVTATLRIPAELFDSVADNLIENALAKRSGTRPVDVEVRFAASEGGVLSVCDNGP
                     PVARAVASRLFEAAVPSQTGLGIGLYHAAKQAERLGYRLELTENEPGRVHFSLGRSAR
                     ALTEAVPLRSIG"
     gene            2947..>3318
                     /locus_tag="VFY80_00525"
     CDS             2947..>3318
                     /locus_tag="VFY80_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6006411.1"
                     /translation="MTHQIATIARYTLLEALRTRLPHVLISIVAILGAVSLFVGAIAI
                     AESTRMQIGTYAASMRLATVFVAAMYVLASVSREFNDKVLDVALALDLPRSHYILGKL
                     SGFVLVATGLALVAALPLAFLA"
BASE COUNT          656 a         1091 c         1018 g          553 t
ORIGIN      
        1 caactgagcg gacgcgttcc gcgtttcttc gccgactggt gcaagaagaa tccggcggat
       61 ccggactgga acggcgtcag ttggccagcg tggttcttcg ccaacaactg gcacgaggtg
      121 gtctactatg ccgtggccca aaaatgcgtg aatccggcgt ccgccgggtg tagtgcggca
      181 ggggcattcc tcacggtgta tggcctgccg ccgcccaacg acaacatccg tgcgattgtc
      241 atcacgccgg ggcgcgcata cgcaggccaa actcggccgt gcgcggcagc aagcgattgt
      301 ctcgaagatc ccgagaacac caacgccgac gactattatc gcaagtcgtc ggtgacaccg
      361 accgtgaacg accgcttgct cgtggtgtcg ccgtgaagtt cgcattgcac aaagcgggat
      421 tcactctgat cgagctggca gtggcgatct tcgtcctgat gctgctgctg ggcagcctca
      481 tcgttccgct cacgacgcag gtggaacagc gcaaggtaag cgagacgcgc aaggcactcg
      541 acgaggcccg cgaggcactg ctcggatttg cagcggcgaa cggtcgactg ccgtgcccgg
      601 catcatcggc gagcaacggc aacgagagct ttgccgcggg cggaagcgcc gccgacggca
      661 actgctccaa tttttttgat ggctttctgc ccgccgccgc cctcgggctc gcgcccacgg
      721 acgccttggg gtttgcggtg gacggatggg gtctcgttca gaatcgcata cgctatgccg
      781 tctcaaaaca gaccgttaac ggcgtgacga acgcgctcac gcgtagcggc ggcatgagcg
      841 cagcgggtct tgcaagcctc gccgcttcgc agctcctcta cgtatgcgga agcggtatcg
      901 gggtgaatgc gggaacgaat tgcggaactg ctgtgacgct ctcgtcgaat gcggtggcga
      961 tcatctactc cgttggcgcg aacgcagcca ccgggggcac cagcgcgcac gaacttgaga
     1021 acccgaatcc caacggcggc agcgccgacc gcattttcgt aagccgcgac tggagtaccg
     1081 cgtcgggttc cgagttcgat gatatggtaa cgtggatcgg gacgaacccg cttttcagcc
     1141 gattgatcgc agcgggacag cttccgtgag cgcgcgggcc gagcggccca aggaaaaatg
     1201 aacccggccc ggctcgtttt cggtgagctc cagccgatag ccaagacgct ccgcctgctt
     1261 ggcggcgtgg taaaggccaa tgccaagccc cgtctgcgag ggcaccgcag cctcgaaaag
     1321 acgtgaagcg accgcccggg ccactggcgg cccgttatcg cacacggaaa gcacaccgcc
     1381 ttccgacgca gcgaatcgca cctcgacgtc cacgggtcgc gtcccgctcc tcttcgccag
     1441 cgcattctcg atcaggttgt ccgccacgct atcaaaaagc tcagccggaa tgcgcagcgt
     1501 cgctgtcacg gtctccggca cgaacttcac gccgcgcgtg gcaaagcgct gcttgagccc
     1561 ttcccaccag atcgcggcct ccactacgtc ggcctcggcg gggtgcgggg tacgcagctt
     1621 gtccagtgtc gcgttgagcc gctgcgtgat ctgaggcagc tggcgctgca tgagtgcctg
     1681 caaggctgct gcctcaccgg cgtcgctcgc ctcggcggcc gcgcacaggg agcgcagcga
     1741 ttgcagcagg tttttgacgt cgtgcgtgag ccgcgcaccg gtttcgtaca ccgcctgcgt
     1801 ataggcggtt tggcgctgga tcagttcacg acgctttgcg tcgtaaaagt gaccgaccat
     1861 ctgtgcgagc agcctcagat gcagcagaac cgccggcgtg agcgcccagc gcgtagagac
     1921 cttcagggtg agatcgcgta tgacgaacac ggatgtgtgc tccgaaggct cgccgaactc
     1981 accccgcccc gactgcgttt cccaccgcac cccgccaacc cacggcaggg tcaacatgtg
     2041 ctgcagggcc tgcgccagaa agcgctgcgg ctctgattcc tgctcggcgt agctcgcaag
     2101 ttgctgcacc cagcgctcga agggcagccc caggctcatg acatagcgcg acagcatgtg
     2161 acccagcccc gcaaatccgc tgtgcgggtt ccacagccag gacagcgcga cgagcaggaa
     2221 cgccatcacg aagagcgtct gggcgagcgc aagcagatat atgccctggc tcaggacctt
     2281 gacgacgaag cttcccagca cgaccaccat gaccagcagg aagagcagca cactataaaa
     2341 gaggtccact gcaacgggac cttcggccgg ccgcgattcg aagcgcgtaa ggaggatcgc
     2401 aaccggcacg aaaaagaggc cgaagcgcac gagcaggaca tgcatcgcat cgggaacttc
     2461 atctgaaaaa agatgcggta ccgcccagat gagcaacagc gacagcacat aaaaagcagc
     2521 gagcagcgag gcgcggcgtt gccggcggtc cgccgtgccc ggcaccgaac cgccgatgag
     2581 accgaacagc acggcaagcc acaccgctat cagccaccag ctgttccatg cggcaaggac
     2641 ggtacccgcg acaaccacca gaatggcgtg gcgccgatcg atctcctgtt cgccgcgcca
     2701 gacgggctgc cacagcagga acagcccgaa gtgagcaagc agcgcgcccg gaacccacca
     2761 cgcgtggatg ccccatgcga gcgcggcatg cagcgcaatc aacatcaatg cgatggacca
     2821 ctgcggatgg gcagtgatga gccgctgtgc cagaagcgcg gatgcgttca tcgggcacgg
     2881 ccttcccggc agacgtaccc gctacgcact ttcgctatac tttcgtccat ccaggcgaga
     2941 cctcccgtga ctcaccagat cgccaccatt gcccgctaca cgctgctcga ggcgctgcgc
     3001 acacgtctgc cgcacgtcct catctccatc gtcgcgatcc taggcgctgt gagtcttttc
     3061 gtaggcgcca tcgccatcgc cgaaagcacg cgcatgcaga tcggcacgta tgccgcgagt
     3121 atgcgcctcg cgacggtatt cgtggccgca atgtacgtcc tcgcaagcgt ctcgcgcgaa
     3181 ttcaacgaca aggtgctcga cgtcgcgctg gcactcgatc tgccgcgcag ccattatatc
     3241 cttggaaaat tgtccggctt cgtgctcgtc gccaccgggc ttgcattggt agccgcgctc
     3301 cctcttgcct ttctggcc
//
LOCUS       DASURG010000033         5836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1132025, whole genome shotgun sequence.
ACCESSION   DASURG010000033 DASURG010000000
VERSION     DASURG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132025"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="rpoB"
                     /locus_tag="VFY80_00530"
     CDS             <1..979
                     /gene="rpoB"
                     /locus_tag="VFY80_00530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923551.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6006412.1"
                     /translation="SLAQTKSLFDKMFDEKKKKLTQGDELPPGVQKMVKVYLAVKRRL
                     QPGDKMAGRHGNKGVISKIVPVEDMPYMADGTTVDIVLNPLGVPSRMNVGQILETHLG
                     WAAKGLGLKIGEMLRAQANAAEVKKFLNEIYNHSGRTEDISVLNDGEALELARNLQQG
                     VPFATPVFDGATEHEIKRMLDLAGMPRSGQVTLHDGRTGDAFERSVTVGYMHMLKLHH
                     LVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKS
                     DDTEGRRKVYESIVKGDHRIEAGMPESFNVLVKEIRSLAIDIDLDHNRY"
     gene            1057..5292
                     /gene="rpoC"
                     /locus_tag="VFY80_00535"
     CDS             1057..5292
                     /gene="rpoC"
                     /locus_tag="VFY80_00535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6006413.1"
                     /translation="MKALLDLFKQVTQEEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVIDPGMTPLKRCQLM
                     TEDDYLAKVEEFGDDFSAAMGAEGVRELLRNLDLRAEIETLRTELASTNSDAKIKKIA
                     KRLKVLEAFNKSGIKPEWMIMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLEVMGLAT
                     TIKAAKRMVEAEEPIVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLSSNNILSPANGEPIIVPSQDIVL
                     GLYYISREKMGARGEGSRFINVAEVSRAYDTGQVEVNARIEVRIREYEVDEVGERREK
                     VTRYQTTVGRALLSEILPAGLPYELINKPLKKKEISRLINASFRRCGLRETVIFADKL
                     MYAGFTMATRAGLSISVDDMLVPAQKNELIAESEKEVQEIEKQYTSGLVTQGERYNKV
                     VDIWGRAGDLVAKAMMEQLGVEPVEDWDAKTGQRVQRKDKSGKPVMQESFNSIYMMAD
                     SGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLAD
                     TALKTANSGYLTRRLVDVTQDLVVTEDDCGTSQGVSMKALIEGGEVVESLRERILGRV
                     CANDVINPESSQVLYPANTLLDEDAVDAIEAAGIDEVKVRTPLTCETRYGLCAKCYGR
                     DLGRGTPVNVGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVVSQVESKSNGVVR
                     YSAGMRYVTNSRDELVAISRNGEVLIQDENGRERERHKVPYGATLMARDGESVKAGQV
                     LVTWDPHTRPIITEYAGRIKFENVEEGVTVARQVDEVTGLSTLVVVDPKRRGSVQAKG
                     LRPQVKLLDAQGQEIKIAGTDQPVNITFQIGSIITVRDGQDVGVGEVLARIPQETSKT
                     RDITGGLPRVAELFEARSPKDAGMLAEVTGTVSFGKDTKGKQRLVITDLDGVAHEYLI
                     PKDKHVMAHDGQVVNKGEMIVDGPADPHDILRLLGVEELARYICNEVQDVYRLQGVKI
                     NDKHIEVIVRQMLRRVQIVEPGETRFITGEQLERAEVLEENERVTVEGKKPGIYEYML
                     LGITKASLSTDSFISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGLA
                     YHRIRKKQAAGTDMGEAIEPAAEIPAESHEQVA"
     gene            5484..>5836
                     /gene="rpsL"
                     /locus_tag="VFY80_00540"
     CDS             5484..>5836
                     /gene="rpsL"
                     /locus_tag="VFY80_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6006414.1"
                     /translation="MPTINQLVRGARRSPPTKSKVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHVVRGSL
                     DTAGVKDRKQGRSKYG"
BASE COUNT         1230 a         1743 c         1942 g          921 t
ORIGIN      
        1 aagcctcgcg cagaccaagt cgctgttcga caagatgttc gacgagaaga agaaaaagct
       61 gactcagggt gatgagctgc cgcccggagt ccagaaaatg gtcaaggtat acctggcggt
      121 caagcggcgc ctgcagccgg gcgacaagat ggcgggacgc cacgggaaca agggcgtcat
      181 ctcgaagatc gtaccggttg aggacatgcc ctacatggcg gacgggacca ccgtcgacat
      241 cgtgctgaat ccgctcggcg tgccctcgcg catgaacgtc gggcagattc tcgagaccca
      301 cctcggctgg gcggcaaaag ggctgggact caagatcggc gagatgctgc gggcgcaagc
      361 caacgctgcg gaagtgaaga agttcctgaa cgagatctac aaccacagcg gccgcaccga
      421 ggacatcagc gtcctcaacg acggcgaagc gctggagctc gcccgcaacc tgcagcaggg
      481 cgtgccgttt gcgacgcccg tgttcgatgg cgcgaccgag cacgagatca agcgcatgct
      541 ggatcttgcg ggcatgccgc gctctggcca ggtcacgctt cacgacggtc gcaccggtga
      601 cgccttcgag cgttccgtga cggtggggta catgcacatg ctgaagctgc accacctggt
      661 ggacgacaag atgcatgccc ggtcgacagg tccgtacagc ctcgtcacgc agcagccgct
      721 cggcggcaag gcgcagttcg ggggccagcg cttcggcgag atggaagtct gggcgctgga
      781 ggcgtacggt gcggcgtaca cgctgcagga gatgcttacg gtcaagtccg acgacaccga
      841 gggccggcgc aaggtctacg agtcgatcgt caagggcgac caccgcatcg aagccggcat
      901 gccggaatcg ttcaacgtcc tggtgaagga gatccgctcg ctcgcgatcg acatcgacct
      961 ggaccacaac cgttattgag atttgcaggg tgaagagtga agagtgcagt acccaaggcc
     1021 cctcgtcatg cctcgcgttt cacctaggag tttgaaatga aagcactgct tgatctattc
     1081 aaacaggtaa cgcaagaaga agaattcgac gccatcaaaa tcgggcttgc atcgcccgag
     1141 aagatccgct cgtggtcgta cggcgaagtg aagaagccgg agaccatcaa ctaccgcacc
     1201 ttcaagccgg agcgggatgg actcttctgc gccaagatct tcgggcctgt gaaggactac
     1261 gagtgcctgt gcggcaagta caagcgcctg aagcaccgcg gcgtcatctg cgagaagtgc
     1321 ggcgtcgaag tgacgctctc caaggtgcgg cgcgagagga tggggcacat cgagctggcg
     1381 agccccgtcg cgcacatctg gttcctgaag tcgctgccct cgcggctcgg catggtgctc
     1441 gacatgacgc tgcgcgatat cgagcgcgtg ctgtacttcg aagcctacgt cgtgatcgat
     1501 cccggcatga cgccgctcaa gcgctgccag ctgatgaccg aggacgatta cctcgcgaag
     1561 gtcgaggagt tcggcgacga cttttccgcg gctatgggcg cggaaggcgt gcgggaactg
     1621 ctgcgcaacc tcgacctccg ggcggaaatc gagacgctgc gcaccgagct cgcttccacc
     1681 aactcggacg cgaagatcaa gaagatcgcg aagcgcctga aggtactgga ggcgttcaac
     1741 aagtccggca tcaaacccga gtggatgatc atggaggtgc tcccggtgct gcccccggag
     1801 ttgcgtccgc tcgtgccgct cgacggcggt cgcttcgcca cctcggacct gaacgacctc
     1861 taccggcgcg tcatcaaccg caacaacagg ctcaagcgcc ttctggagtt gaaggcgccc
     1921 gaaatcatcg tgcgcaatga aaagcgcatg ctgcaggaag cggtcgattc gctgctcgac
     1981 aacggccgcc gtggcaaggc gatgaccggc gccaacaagc gcccgctgaa gtcgcttgcc
     2041 gacatgatca agggcaaggg cggacgtttc cggcagaacc ttctcggcaa gcgtgtggac
     2101 tactccggcc gttcggtgat cgtggtgggg ccgcaactga agctccacca gtgcgggctg
     2161 cccaagaaga tggcactcga actcttcaag ccgttcatct tcaacaagct cgaagtgatg
     2221 ggcctcgcca cgacgatcaa agccgcgaaa cgcatggtcg aggcggaaga gccgatcgta
     2281 tgggacatcc tcgaggaggt gatccgcgag catccggtcc ttttgaaccg cgcgccgacg
     2341 ctgcaccgtc tcggcatcca ggcattcgag ccggtgctga tcgagggcaa agcgatccag
     2401 ctccatccgc tcgtgtgtgc cgcgttcaac gcagacttcg atggcgacca gatggcggtg
     2461 cacgtgccgc tctcgctcga ggcgcagatg gaagcgcgga cactcatgct ctcctcgaac
     2521 aacatcctct cgcccgcgaa cggcgagccg atcatcgtgc cctcgcagga catcgtgctc
     2581 ggtctctact acatcagccg cgaaaaaatg ggcgcgcgcg gcgagggctc gcgtttcatc
     2641 aacgtggcgg aagtctcgcg cgcctacgat accgggcagg tggaggtgaa cgcccggatc
     2701 gaagtacgca tcagggaata tgaggtggat gaagtcggcg agcgccgcga aaaggtgacg
     2761 cgctaccaga cgacggtcgg ccgcgcgctc ctttccgaaa tcctgcccgc ggggctgccc
     2821 tacgagctga tcaacaagcc gctcaagaag aaggagatct cgcggctgat caacgcgtcg
     2881 ttccgccgct gtggcttgcg cgagacggtg atctttgccg acaagctcat gtacgcgggc
     2941 ttcaccatgg caacgcgcgc tgggctctcg atttcggtcg atgacatgct ggtgccggcg
     3001 cagaagaacg agttgatcgc cgaatcggaa aaagaagtac aggagatcga gaagcagtac
     3061 acctcggggc ttgtcacgca gggcgagcgc tacaacaagg tggtggacat ctggggccgt
     3121 gcgggcgacc tcgtggcgaa ggccatgatg gagcagctcg gcgtcgagcc ggtcgaggac
     3181 tgggacgcga agaccggcca gcgggtgcag cggaaggaca agagcggaaa gccggtgatg
     3241 caggaatcgt tcaactcgat ttacatgatg gccgattcgg gcgcgcgcgg ttccgccgcg
     3301 cagatccggc agctcgccgg gatgcgcggt ctcatggcga aaccggacgg gtcgatcatc
     3361 gagaccccga tcaccgcgaa cttccgcgaa ggcctgaacg tcctgcagta cttcatctcg
     3421 acgcacggtg cacggaaggg cctcgcggat accgcgctca agacggccaa ttccggctac
     3481 ttgacgcgcc gtctcgtgga cgtgactcag gatctcgtcg tgaccgagga cgactgcggc
     3541 acgagtcagg gcgtctcgat gaaggcgctg atcgaaggtg gtgaagtcgt ggagtcactg
     3601 cgcgagcgca tcctcggacg cgtatgcgca aacgacgtga tcaatccgga atcgagccag
     3661 gtgctctacc cggccaatac gctgctcgac gaggatgcgg tggacgcgat cgaggcggct
     3721 ggcatcgacg aagtgaaggt gaggacgccg cttacgtgcg aaacgcgcta cggattgtgc
     3781 gccaagtgct atggccggga tctcggccgc ggcacgccgg tcaacgtcgg cgaggcggtc
     3841 ggcgtgatcg cagcgcaatc gatcggcgag cccggcacgc agctcaccat gcgcaccttc
     3901 catatcggcg gcgcggcctc gcggaccgcg gtcgtgagcc aggtcgagag caagtcgaat
     3961 ggcgtcgtgc gttacagcgc tggcatgcgt tacgtcacca attcgcgcga cgagctcgtt
     4021 gcgatctcgc gcaatggcga ggtgctgatc caggacgaga acgggcgcga gcgcgagcgt
     4081 cacaaggtgc cgtacggcgc aacgctcatg gcgcgcgacg gcgagagcgt gaaagcgggt
     4141 caggtgctcg tgacctggga tccgcatacc cggcccatca tcacggaata cgccggccgc
     4201 atcaaattcg agaacgtcga ggagggggtc accgtcgccc gccaggtgga tgaagtgacc
     4261 gggctctcaa ccttggtggt cgtcgatccc aagcgccgcg gcagcgtgca ggcgaagggc
     4321 ttgcggccgc aggtgaagct gctcgacgcc caggggcagg agatcaagat cgcgggcacc
     4381 gaccagccgg tcaacatcac tttccagatc ggctcgatca tcacggtgcg cgacggtcag
     4441 gacgtgggcg tgggcgaagt gctcgcgcgc attccgcagg agacctcgaa gacccgcgac
     4501 attaccggcg ggcttccgcg cgtagcggag ctcttcgaag cacgctcgcc caaagacgcc
     4561 ggcatgctcg ccgaagtgac cggcacggtg tcgttcggca aggatacgaa aggcaagcag
     4621 cgcctcgtga ttacggatct cgacggtgtc gcgcacgagt atctgatccc gaaggacaag
     4681 cacgtgatgg cgcacgacgg gcaagtcgtg aacaagggcg agatgatcgt ggacggaccg
     4741 gccgatccgc atgacatcct gcgtctgctc ggcgtcgagg agcttgcccg ctacatctgc
     4801 aacgaggtgc aggacgtcta ccggctgcag ggtgtgaaga tcaacgacaa gcacatcgaa
     4861 gtgatcgtgc gccagatgct gcgccgggtg cagatcgtcg aaccgggtga gacccggttc
     4921 atcacggggg agcagctcga gcgcgccgaa gtcctcgagg agaacgagcg tgtcacggtg
     4981 gagggcaaga agccggggat ctacgagtac atgctgctcg gcatcaccaa ggcctcgctt
     5041 tcgaccgatt cgttcatctc cgcagcgtcc ttccaggaga cgacgcgtgt cctgaccgag
     5101 gcggcgatca tgggcaagcg cgacgagctg cgcggtctca aggaaaacgt catcgtgggc
     5161 cgactcatcc cggccggaac gggcctcgcc taccaccgaa tccgcaagaa gcaggccgcg
     5221 ggtacggaca tgggcgaagc gatcgagccg gcagccgaaa ttccggcgga aagccacgag
     5281 caggttgctt gacagagatg cggtaagttc atagaattct cggtcttttt agccgcttaa
     5341 ccccttcgat ccgggcggcg gcaaaaccac gaacgggatc aggcagggac ggtggagacg
     5401 ccgtccccgc ttttttgggc atcggaaaac agcgaacacg cggtgcgtga aagccccgcg
     5461 aaacgacagg acagcggaga aacatgccaa caatcaacca actcgtgcgc ggcgcccgcc
     5521 gctcgccgcc gacgaaaagc aaggtgccgg cgctcgcggg cagcccgcag aagcgtgggg
     5581 tgtgcacgcg tgtgtacacg acgacgccga agaagcccaa ttcggcgctc cgtaaggtgg
     5641 ccaaggtccg gctcaccaac ggcttcgagg tgatcggcta tatcggaggc gaaggccaca
     5701 atttgcagga gcactcggtg gtgctgatcc gcggcggccg ggtgaaggac ctccctggcg
     5761 tgcgctacca cgtcgtgcgc ggaagcctcg ataccgcagg ggtgaaggat cgcaaacagg
     5821 gccgttccaa gtacgg
//
LOCUS       DASURG010000034         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_315814, whole genome shotgun sequence.
ACCESSION   DASURG010000034 DASURG010000000
VERSION     DASURG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315814"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFY80_00545"
     CDS             complement(<1..1034)
                     /locus_tag="VFY80_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6006415.1"
                     /translation="MSSAAASGFGRLLNPRGIAVVGASGDPRRIGSEPVRILQQTGYR
                     GAIYPVNPKYGELNGLACYPDLAALPQPCDLAIVAVNAALVPSVIRECGQARIPFAIV
                     FSAGFREIGATGSALEGDLRRAARETGVRVVGPNCIGMMNLIDRVYCGFGAGFANAKL
                     KSGPVAFVSQSGGFAFSVVGLADFEGIGFSYVVSSGNEADITTLDWIDDFLDRGDVEI
                     VVSYMEGVSDGRRLRALGRRALELKKPILVWKVGNSTAARAAAESHTASMTADYQLYR
                     TAFDEGGFIEIEDVHDLVDATRAFLSRRLPRGGNVAVITTSGGSGVLTADVCERHGLT
                     LPALTPGTIE"
     gene            complement(1031..2002)
                     /locus_tag="VFY80_00550"
     CDS             complement(1031..2002)
                     /locus_tag="VFY80_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006416.1"
                     /translation="MKAALLTVAALNALAIAAASAQTTQAYPARAVRIIVPYAAGGNT
                     DITARAIGGKLSEVFGQQVIVDNRPGGATNIGSELAAKAPADGHTLFMGGASNAINMS
                     LYAKPPYDTLRDFAPITLCVKGANVLSVHPSLPARTLKELIGLAKARPGQLNFASSGL
                     GSSNQMAGELLKVMAGIHLVHVPYKGNAPALTDTVAGHVHMIFSGVPALVPHIQSGRL
                     RAIAIGSLKRFPAIPDVPTFDESGVKGYEATTWFGLMAPIRTPKDIVSRLNAEVTKII
                     ASPEISKRFVNDGLEPIGNSPAEFAQFIRDEIAKYEKVIKAAKITPL"
     gene            complement(2022..>2881)
                     /locus_tag="VFY80_00555"
     CDS             complement(2022..>2881)
                     /locus_tag="VFY80_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6006417.1"
                     /translation="STRVEAAFFSGDVFPEYSRQFFSAARKAPNLKWLHVFNTGVDHP
                     IYAEMLARGVRLTTSAGATAQSIAHTAIGALLMLARGFPHWLTAQRRHVWTPLEGADI
                     PRDLAEQTVVVLGLGHIGREFARIARTLGLHVIGVRRSGARPDDPVDESHSPDRLAEI
                     LERADWLLIACPLTHETRGLIDARMLAALRRGARVINVARGEIVDEGALVAALRSGHL
                     AGAYLDVFENEPLPPDSPLWEMPNVFVSPHNSSAASGNDARVYAIFQDNLERWMRGAP
                     LVNEVHRAH"
BASE COUNT          517 a          919 c          937 g          508 t
ORIGIN      
        1 ttctcgatcg tcccgggtgt cagcgcggga agcgtgagcc cgtgccgctc gcacacatcg
       61 gccgtcagca cgcccgaacc gccggaagtc gtgatgaccg cgacgttgcc gccgcgcggc
      121 aggcgccgcg acagaaacgc gcgcgtcgcg tccacgaggt cgtgcacgtc ctcgatttcg
      181 atgaatccgc cttcgtcgaa agccgttcgg tagagctggt agtccgcggt catgctggcg
      241 gtatgagact ccgctgcagc gcgcgcggcg gtggaattgc cgaccttcca gacgaggatg
      301 ggctttttca gctccagtgc gcggcgtcca agtgcccgaa ggcgccgtcc gtcggacacg
      361 ccttccatat aactcacgac gatttcgacg tccccgcgat cgaggaagtc gtcgatccag
      421 tcgagcgtcg tgatgtccgc ttcgttcccg ctcgacacca cgtagctgaa gccgatgccc
      481 tcgaaatcgg cgagaccgac gacactgaaa gcgaaccctc cgctttgcga gacgaatgcg
      541 acgggcccgg acttcagctt cgcgttcgcg aatccggcgc cgaagccgca gtacacgcgg
      601 tcgatcaagt tcatcatgcc gatgcagttc ggcccgacca cgcgcacgcc ggtttcgcgg
      661 gcggcacgcc tcaaatcgcc ctccagcgcg gaaccggtcg caccgatttc gcgaaagccc
      721 gcagaaaaaa caatggcgaa cgggatgcgg gcttgtccgc actcgcgtat gacggaaggc
      781 acgagcgcgg cattgacggc gacgatcgcg aggtcgcacg gctgcgggag cgcagcgagg
      841 tccggatagc aggcgagtcc gttcagttcc ccatacttcg ggttgaccgg atagatcgcg
      901 ccccggtagc cggtctgctg caggatcctg accggttcgc tgccgatgcg ccgggggtcg
      961 cccgatgcgc cgacgaccgc gatgccgcgc ggattcagta accgtccgaa tccgctcgcc
     1021 gccgcgctgc tcatagcggc gtgatctttg cggctttgat cactttctcg tacttggcga
     1081 tctcgtcgcg aatgaactgc gcgaattcgg ccgggctgtt gccgatcggc tccagaccat
     1141 cgttgacgaa gcgcttgctg atttccgggc tcgcgatgat cttcgtcact tcggcgttga
     1201 gccgcgagac gatgtccttc ggcgtgcgga tcggcgccat gagcccgaac caggttgtcg
     1261 cctcgtagcc cttcacaccg gactcgtcga aagtcggcac gtcgggaatc gccgggaagc
     1321 gcttgaggct cccgatcgcg attgcccgca gcctaccgga ctggatgtgc ggcacgagcg
     1381 ccggcacgcc gctgaagatc atgtgcacat gacccgcgac cgtatccgtc agcgcgggcg
     1441 cattcccctt gtaaggcacg tgcacgagat ggatgccggc catcaccttc agcagctcgc
     1501 ctgccatctg gttggagctg ccgagcccgc tggaggcgaa gttgagctgt cccgggcgcg
     1561 ctttcgcgag cccgatcagt tccttcagcg tcctcgcagg aagtgaagga tggaccgaca
     1621 gcacgttggc gccctttacg cacaacgtaa tcggcgcgaa atcgcgcagc gtatcgtagg
     1681 gcggtttcgc gtagagcgac atgttgatcg cgttcgaagc gccgcccatg aagagggtat
     1741 ggccgtcggc aggcgccttg gcggcgagtt ccgaaccgat gttggttgcg ccgccgggtc
     1801 gattgtcaac gatcacctgc tgtccgaaaa cttcggacag tttgccgccg atagcccgcg
     1861 cagtgatatc cgtgttgccg cccgccgcgt acggaacgat gatgcgcacg gcgcgcgcag
     1921 ggtaggcctg tgtcgtttgg gcggaagcgg cagcgatggc gagcgcatta agcgcggcga
     1981 cggtcaaaag cgcagctttc atgagtaatc gttctccgaa atcagtgggc gcggtgcact
     2041 tcgttcacga gcggtgcgcc gcgcatccag cgctcgagat tgtcttggaa gatcgcatag
     2101 acgcgggcat cgttgcccga agcggccgac gaattatggg gcgacacgaa gacgttcggc
     2161 atttcccaga ggggtgagtc gggcggcaaa ggctcgtttt cgaacacgtc gagatatgcg
     2221 ccggcgagat gcccgctccg aagggccgca acgagcgccc cctcgtccac gatttcgccg
     2281 cgcgcgacgt tgatcacacg cgcgccgcgt cgcaacgccg ccagcatgcg agcatcgatg
     2341 aggccacggg tctcgtgggt gagcgggcag gcgatcagca gccagtccgc gcgctcgagg
     2401 atttccgcga gacgatccgg cgaatgcgat tcatcaaccg ggtcgtccgg ccgcgctcca
     2461 cttcggcgca ccccgatcac atggaggccg agcgttcggg cgatacgtgc aaattcccgg
     2521 ccgatgtgac ccaatcccag cacaacgacc gtctgctccg cgagatcgcg cggtatgtcg
     2581 gcgccttcga gaggcgtcca cacatgcctg cgctgcgcgg tgagccagtg cggaaaccca
     2641 cgcgcgagca tgaggagcgc gcctatggcc gtgtgtgcaa tgctctgggc cgtcgctccc
     2701 gcggaggtcg tcaggcgcac tccacgcgcg agcatttcgg cataaatcgg atgatccacg
     2761 ccggtgttga agacgtgcag ccacttcaga ttcggcgctt tccgcgcggc agagaagaac
     2821 tggcgcgaat attcgggaaa cacatcgccg gaaaagaatg cggcttcaac gcgcgtgctg
     2881 t
//
LOCUS       DASURG010000035         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_622303, whole genome shotgun sequence.
ACCESSION   DASURG010000035 DASURG010000000
VERSION     DASURG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622303"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFY80_00560"
     CDS             <1..449
                     /locus_tag="VFY80_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6006418.1"
                     /translation="TPGHASNHLCYLLEQRQLLFTGDHVMQGSTVVISPPDGDMQKYL
                     DSLERLLALDIAAIAPGHGQLIDTPHEEARRLIAHRLKREQKVLDAFSRKHPATLDEL
                     LPIVYSDTSPRLHPVARRSLEAHLIKLLREGRVAEKEGAWKPSTAA"
     gene            complement(459..899)
                     /locus_tag="VFY80_00565"
     CDS             complement(459..899)
                     /locus_tag="VFY80_00565"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX6006419.1"
                     /translation="MDRMLAQWRAEEVALNEGAQAMQIESFERLIGVPLAEDVRAFYT
                     AANGMQDYQHDPRMVSMWSLERITRERNIQDGEDEWGRFQDVAFADVLFSAWYFRLRV
                     RENGGSSVLSELTHEELPSLYAMFDAFLQRPGSMGLATPSPARK"
     gene            complement(1064..2041)
                     /locus_tag="VFY80_00570"
     CDS             complement(1064..2041)
                     /locus_tag="VFY80_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006420.1"
                     /translation="MKPLAAATLVLLLALAAVSGTALAQKSAYPDKPIRFVVPFAPGG
                     GTDIIARTMAQKLSDAFGQSVIVDNRGGAGGTIGAEIAAKAAPDGYTLIMVSGSYAVN
                     AGLYKLPYDPLNGIDTVSLVGTTPFIFAVNPSVPAKSVKELIALAKSKPGTLNYASTG
                     TGGITHMTTELFRLMSGINIVHIPYKGTGPALTDVIGGQVQMIFGAILATLPHVKSGK
                     LRGLAVTGPTRTDAAPSLPTVAESGVPGYEVTLWYGVWGPKGLRKDVIARWHSELTRI
                     LQSPDMKARLATEGLEAVGSTPEEFRAHLKAEIAKWAKVIKAANVKVGS"
     gene            complement(2038..3426)
                     /locus_tag="VFY80_00575"
     CDS             complement(2038..3426)
                     /locus_tag="VFY80_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6006421.1"
                     /translation="MNFDRRAVSAPTREVAAWISGLRYEDLPQRTREVVRSALLDTLG
                     CGVYGFNTSWAGLLLEWARAGGTGGECTVWGEAQPSLRAADAALVNGTASHAFELDDY
                     HNAKLHPGAVVVPAAVALAEKLGASGARLVTAIAAGYEVMIRSSLALNPSAARLRGWH
                     LTGVCGPFGAAAACASLLGFDEEHTAWALGLAGTQGAGLWAFNADGTMSKRLHAGKAA
                     HSGVLAAELAAMGFTGPTQIYEYHDGGVLKAFSDESDPAPLTRDLGTHFHLDTTSIKP
                     YSCCGSTHAYVDAALELRRKLGSPWDTRRRVKIGLSKVVDVQCGFPYAPSTALNAQMS
                     LRYIVAAALTDGQALPPQFTQQRIADPEITRLAASLELVPDPRLDGLYPAHFAGWVAA
                     EANGDWLRADILDPSGSPAAPLDARAITEKFRGINPQLPTDRIAAVALDIERHTVSDL
                     ITLLVHRGQESR"
     gene            3640..>4376
                     /locus_tag="VFY80_00580"
     CDS             3640..>4376
                     /locus_tag="VFY80_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6006422.1"
                     /translation="MDYDVIVVGAGNAAFSAAVSAQENGAKRVLVLEKAPREKRGGNT
                     HFSGAVFRFIYDDVSELDRFVPNAERDYPGFHKGVQPYPVEAFHADLMRVTQDRSDPE
                     LSKILIENSYDTMCWLQERGRMEFELAVSVMGVRVGNEVKWPRGAMIRTVHEGVGLSK
                     TWFDTAEKTGIEIRYESGARALTQAESGRVNGVVVQGPNGIETIHAKAVVLGCGGFET
                     NPAWRRLYLGDPWDHAKVRGSNFNYGEG"
BASE COUNT          763 a         1406 c         1420 g          787 t
ORIGIN      
        1 acacgcccgg tcatgccagc aaccatctct gctatctgct ggagcagcgg cagcttctct
       61 ttaccggcga ccacgtaatg cagggctcga cggtcgtgat ctcgccgccc gacggcgaca
      121 tgcagaagta tctggactcg ctcgagcgcc tgcttgcgct cgacatcgcg gcgatcgccc
      181 ccggacacgg tcagctcatc gacactccgc atgaagaggc gcgtcgattg attgcgcacc
      241 ggctgaagcg cgagcagaag gtgctcgacg ctttttcgcg taagcatccg gcgacgctcg
      301 acgaactttt gccgatcgtc tactcggata cgtcaccgcg cctgcatccg gttgcacgcc
      361 gctcgctgga agcgcatctc atcaagctct tgcgggaagg gcgtgtggcc gaaaaagagg
      421 gcgcatggaa accctcgacg gccgcctgac gaagcgatct atttccgtgc gggggacggt
      481 gtggcgagcc ccatactgcc gggccgctgc aggaacgcgt cgaacatcgc gtacaggctg
      541 ggcagctctt cgtgcgtgag ttcagagagc acgctgctgc cgccgttttc gcgcacgcgt
      601 aggcgaaaat accacgccga aaacagcacg tcggcgaagg cgacgtcctg gaagcggccc
      661 cattcgtcct cgccgtcctg gatgttgcgc tcgcgtgtga tgcgctcgag cgaccacatc
      721 gacaccatgc gggggtcatg ctggtaatcc tgcatgccgt tcgcggcggt gtagaacgcc
      781 cgcacgtctt cggcgagcgg cacgccgatc aggcgctcga atgattcgat ctgcatcgcc
      841 tgggcgcctt cgttgagcgc gacctcttcc gcgcgccatt gcgccagcat ccggtccatc
      901 gagttcatgc tcctccccgc ttgttgaagt tccgcgactg cgcatcgcgc agtcccattt
      961 tcaggacagc ttcattttcg tgccgcagga gtataagcac gtcgccccaa gcggtatgta
     1021 ggacaaagga cgacgcgccg cgaatggtgg ccattccatg cgtttagctc ccgactttca
     1081 cgttagccgc tttgatgact ttcgcccact tggcgatctc cgccttgaga tgcgcgcgaa
     1141 attcctcggg cgtgctgccc acggcttcca gtccttccgt cgcgaggcgc gccttcatgt
     1201 ccggcgactg caggatgcgc gtgagctcgg aatgccagcg tgcgatcacg tcttttcgca
     1261 ggcccttggg tccccacacg ccgtaccaca acgtcacttc gtaacccggc acgcccgact
     1321 cggcgacggt cggcagactg ggcgcagcat cggtgcgtgt cggtcccgtc acggcgagcc
     1381 cgcgcagctt gccgctcttc acatgcggca gtgtcgcgag gatggcgccg aagatcatct
     1441 gcacctgtcc gccgatcacg tcggtcaacg ccggaccggt gcccttgtat ggaatgtgga
     1501 cgatgttgat gcccgacatg agacggaaga gctcggtcgt catgtgcgtg atcccgccgg
     1561 tgccggtgga cgcgtagttg agcgtgccgg gcttcgactt ggcgagcgcg atcagttcct
     1621 tcacgctttt cgccggcacg gaaggattga ccgcgaagat gaagggcgtg gtgccgacga
     1681 gggatacggt atcgattccg ttgaggggat catacggcag cttgtagagc ccggcgttca
     1741 cggcgtagct gcccgatacc atgatcagcg tgtagccgtc cggtgcagct ttcgcggcga
     1801 tctccgcgcc gatggtgcct ccggcgccgc cccgattgtc cacgatgacc gattgcccga
     1861 acgcgtctga cagtttttgt gccatggtgc gcgcgatgat gtccgtgccg ccacccggcg
     1921 cgaatggaac gacgaaccgg atgggcttgt cgggatacgc gctcttctgc gcgagcgcgg
     1981 tgccggaaac cgcggcaagc gcgagcagca gcacgagtgt cgcggcggcg agcggcttca
     2041 tcggctctct tgcccacggt gcaccagcaa cgtgatgagg tcgctcaccg tatggcgttc
     2101 gatatcgagc gcgacagcgg cgatgcggtc cgtcggcagt tgcggattga tgccgcgaaa
     2161 cttttccgtg atcgcccgtg catcgagcgg agccgcaggc gagcccgatg gatcgagaat
     2221 gtcggcgcgt agccagtcgc cgttcgcttc cgccgccacc caaccggcga agtgcgccgg
     2281 atagagcccg tcgagccgcg gatcgggcac gagctccaag cttgcggcca gccgggtgat
     2341 ctccgggtcc gcaatccgct gctgcgtaaa ctgcggcggc agggcctgtc cgtcggtgag
     2401 cgctgccgcg acgatgtatc gcaggctcat ctgcgcattg agcgcggtgc tcggcgcgta
     2461 gggaaaaccg cattgcacat ccacgacttt gctcagcccg atcttgaccc ggcggcgcgt
     2521 atcccacgga ctgccgagct tgcgcctcaa ttcgagcgcg gcatcgacgt aagcatgcgt
     2581 gctgccgcag catgagtacg gcttgatgga ggtcgtgtcg agatggaagt gcgtgcccag
     2641 atctctcgtg agtggggccg ggtccgactc gtccgaaaag gccttcagca cgccgccatc
     2701 gtgatattcg tagatctggg tgggtccggt gaaccccatc gcggcgagct ctgccgccag
     2761 cacgccagag tgcgccgcct tgccggcgtg cagacgcttg ctcatcgtac cgtcggcgtt
     2821 gaacgcccac agtccggcgc cctgtgtgcc ggcaagtccg agcgcccatg cggtgtgttc
     2881 ctcatcgaaa cccaggagcg acgcgcatgc ggctgctgcg ccgaacgggc cgcacacccc
     2941 ggtcaaatgc cagccgcgaa ggcgggccgc ggagggattg agcgcaaggc tcgagcgaat
     3001 catcacctca tagcccgccg caatcgcggt gacgagccgg gcgccgctcg caccgagctt
     3061 ttcggccagc gcgaccgccg cgggtacaac caccgcgccg ggatggagct tggcgttgtg
     3121 atagtcgtcg agctcgaatg catgtgaggc ggtgccgttg acgagcgcgg cgtccgctgc
     3181 gcgcagcgac ggctgcgctt cgccccacac ggtgcactca ccacccgttc cgccggcgcg
     3241 cgcccattcg agcagcaggc ccgcccacga cgtgttgaat ccgtatacgc cgcacccgag
     3301 cgtgtcgagt agcgcgctgc gcaccacctc gcgcgtgcgc tgcggaaggt cctcgtagcg
     3361 caacccggaa atccatgcgg ccacttcacg ggtaggggcc gaaacggcgc ggcgatcgaa
     3421 attcatgcgg gcctccgata catgtgatcc agcattcgag acgcatgctg attgtagatt
     3481 caacgctgcg ctcgtgtcat tcgatccgac cgccttgaca ctcttttgga gcgaaccgca
     3541 tactcgcaac cgtccgtcgc ggccgggcct aaagtccact atccggtcgc gcccccgcta
     3601 ataccacggc cccggagacc acagagggag tttcgcaata tggattacga tgtcatcgtc
     3661 gtcggcgcag gcaacgcggc cttcagcgcg gcggtatcgg cgcaggagaa cggcgcaaag
     3721 cgggtgctgg tgctcgagaa agcgcccagg gaaaagcgcg gcggcaacac gcacttcagc
     3781 ggtgcggtct tccgcttcat ctacgacgat gtctccgaac tcgatcgctt cgtgccgaac
     3841 gcggaacgcg attaccccgg tttccacaag ggcgtccagc cctatcccgt ggaggcattc
     3901 cacgcggacc tgatgcgcgt cacacaggat cgcagcgatc ccgagctctc gaagatcctg
     3961 atcgagaact cgtacgacac gatgtgctgg ctgcaggagc gcggaaggat ggagttcgag
     4021 cttgcggtct cggtcatggg cgtgcgtgtc ggcaatgaag tcaagtggcc gcgcggggcg
     4081 atgatccgca ccgtgcacga gggggtcggc ctgtcgaaga cgtggttcga tacggccgaa
     4141 aaaaccggca tcgagatccg ttacgaaagt ggcgcgcgcg cactcacgca agccgaaagc
     4201 ggtcgcgtga acggtgtcgt ggtgcagggg ccgaacggca tcgagacgat ccatgccaag
     4261 gcggtggtgc tcggctgcgg cgggttcgag accaatccgg catggcgccg gctgtacctg
     4321 ggcgatcctt gggaccacgc caaggtgcgc ggctccaact tcaattacgg cgaggg
//
LOCUS       DASURG010000036         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_111418, whole genome shotgun sequence.
ACCESSION   DASURG010000036 DASURG010000000
VERSION     DASURG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111418"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VFY80_00585"
     CDS             <1..1704
                     /locus_tag="VFY80_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6006423.1"
                     /translation="NMPKTTGSRLFAEMLKGYGITHVFFVPTILMDALAEMDDLGIRK
                     VMTHGEKAAAYMADGYARAKGAPGVCMAQQIGASNIAAGLRDAYMGCVPLIVVSGGST
                     VGGRYRHAYQEVEDFSQFDAVTKLNLQLDDVTRLPDLLRQMFRTATTGAPGPVHMRLR
                     GSHGQNVEVDADLEPLVAERFGRVPAFRPAPEPEAVREAVAALSKAQRPIIVAGGGVV
                     TSQARSELVQLAEKLQIPVATSLTAKDAILDSHPLAVGVAGIYSRACANQAVSEADLV
                     FFVGAHAGGQVTANWKIPAAATPVIQLDIDPQQLGRNYPNTVSLHADAKVGLRHLIDA
                     AQAKPPEAAKSRLARVRELVGQWREGAERMRNSDAVPLRPERICKEITDALPADGVVV
                     SDTGHAGMWTGQMIYITQPGQRYIRCEGSLGWGLPGALGVKCALPDRPVVLFTGDGGI
                     YYHLAELETAARCGINLVVVINNNNSLNQEIPLVKAAYREKRDERSGEIWRFQRGINL
                     AHVAETLGCAGFRVEKPGEVKELLPRAFAMGKPVVIDVASEEQALAPTAWLPGGGRQG
                     Y"
     gene            1761..2744
                     /locus_tag="VFY80_00590"
     CDS             1761..2744
                     /locus_tag="VFY80_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006424.1"
                     /translation="MREWKMCAMLTVALSALAALPAWAQGAAASYPSKPIRLIVPLAA
                     GGPSDTMARTLAQKLGEVVKHNVIVDNRPGASGIIGTELVAKSAPDGYTILLVSSAIS
                     INPSLFRKLPYDTLKDLTAVSLLAGAPYLLAVHPSLPVKNVKQLIALAKARPGELNHA
                     SGGSGTGPHLGMEVFMQRTGIKVVHVTYKGGGPALNDFVAGHTQVYMGNMITLYPQAK
                     ANRIRALAVTSARRSPAAPDIPTVAESGVSGFEEGGHHGIVAPSGVPKEIIAKLNQAI
                     VAAMRSPDIEKRLSVEGAQVIASSPEEYDALIRRDLAKWAKVIAAAGITPQ"
BASE COUNT          520 a          894 c          921 g          450 t
ORIGIN      
        1 aacatgccca agaccaccgg ttcgcggcta ttcgccgaaa tgctgaaagg ctacggcatc
       61 acgcatgttt tcttcgtgcc gacgattctc atggatgcgc tggctgaaat ggacgacctc
      121 ggcatccgca aggtgatgac gcacggcgag aaggcggcgg cctacatggc ggacggttac
      181 gcccgcgcga aaggggcgcc gggtgtatgt atggcgcagc agatcggcgc atcgaatatc
      241 gctgccggct tgcgcgatgc gtacatggga tgcgttccgc tcatcgtcgt gagtggcggc
      301 tcgaccgtcg gggggcgtta ccgccatgcc tatcaggagg tcgaggactt cagtcagttc
      361 gacgcggtga cgaagttgaa cctccagctc gacgacgtca cccgcttgcc cgacctcctg
      421 cggcagatgt ttcgcaccgc cacgaccggc gcgcccgggc cggtgcacat gcggctgcgc
      481 ggctcgcacg gtcagaacgt cgaagtcgat gcggacctgg agccgctcgt ggcggagcga
      541 ttcgggcgcg tgcccgcgtt ccgcccggcc cccgagcccg aagccgtgcg cgaggcggtt
      601 gccgcccttt ccaaagcaca gcgcccgatc atcgtagccg gtggcggcgt cgtcacttcg
      661 caggcgcgga gcgagctggt ccagctcgcg gagaaactgc agatacccgt cgcgacttct
      721 ctcaccgcga aggatgccat tctcgactcc catccgctgg cagtcggggt ggcgggtatt
      781 tactcgcggg cgtgcgccaa tcaggcggtg tccgaagcgg atctcgtgtt cttcgtcggc
      841 gcgcacgcag gcggacaggt gacggcgaac tggaagatcc ctgcggcagc tacaccggtg
      901 atccagctcg acatcgaccc gcagcagctc ggccggaatt atccgaacac ggtttcgctg
      961 cacgccgacg cgaaggtggg cctgcgccac ctgatcgatg cagcgcaggc gaagccgccg
     1021 gaagcagcga agagcaggct cgcgcgcgtg cgcgagctcg tggggcaatg gcgcgaaggt
     1081 gcggagcgga tgcgcaattc agacgcagtc ccgttacggc ccgagcgtat ctgcaaggag
     1141 atcaccgacg cccttcctgc ggacggcgta gtggtgtcgg ataccgggca cgccggcatg
     1201 tggacgggac agatgatcta cattacgcag cctgggcagc gctacatccg ctgcgagggt
     1261 tcgctcggct ggggtctccc cggcgcgctg ggcgtgaaat gcgcattgcc tgaccgaccg
     1321 gtcgtgctct tcaccggaga cggcggcatc tactaccacc tcgccgaact ggaaaccgcg
     1381 gcgcgctgcg gcatcaatct cgtggtggtg atcaacaaca acaactcgct caaccaggag
     1441 atcccgctgg tgaaggcggc ctaccgcgag aagcgcgacg agcggtccgg tgaaatctgg
     1501 cgcttccaaa gaggcattaa cctcgcgcac gtcgcagaaa cactgggctg tgcgggattc
     1561 agggtcgaga agcccggcga agtgaaggaa ctgctgccgc gcgcattcgc gatggggaag
     1621 cccgtcgtga tcgatgtcgc aagcgaggag caggcgctcg cacccacggc atggttgccc
     1681 ggcggcgggc ggcagggtta ttgaccgccc gggatctccc gctgtcccga tttgacgatt
     1741 ccgttcgtaa ggaggatcgg atgcgagaat ggaaaatgtg cgccatgctc acggtcgccc
     1801 tctcagcgct cgccgcgctg ccggcatggg cgcagggcgc ggccgcaagc tatccgtcga
     1861 aacccatacg gttgatcgtt ccgctcgcag cgggcgggcc gagcgacacg atggcacgca
     1921 cgctcgccca gaaactcggc gaggtcgtga agcacaacgt gatcgtggac aaccggcccg
     1981 gggcgagcgg catcatcggc accgaactcg tcgcaaaatc cgcacctgac ggctacacga
     2041 ttctgctcgt atcgagtgcg atttcgatca atccgagcct cttccgcaag ctgccgtacg
     2101 acacgctcaa ggatctgacc gccgtgtcgc tgttggcagg cgcgccctat ctactggccg
     2161 tgcatccctc gctgccggtg aaaaacgtga agcagctcat cgcgctcgcg aaggcgcggc
     2221 ccggtgagct caatcacgcc tcgggcggca gcggcaccgg gccacacctc gggatggaag
     2281 tattcatgca gcgcaccggc atcaaggtcg tgcacgtgac ctacaagggc ggcgggccgg
     2341 cgctgaacga cttcgtggcg ggtcacacgc aggtgtacat ggggaacatg atcacgctct
     2401 atccgcaggc caaggcgaac cgcatccgtg cgctcgctgt cacctcggcc aggcgctctc
     2461 ccgcagcgcc ggatatcccg accgtcgcgg aatccggcgt gtcgggattc gaggaaggcg
     2521 gccatcacgg catcgtggcg ccatcgggcg tgccgaagga gatcatcgcg aagctcaacc
     2581 aggccatcgt cgctgccatg cgctcgcccg acatcgaaaa gcgcctgagc gtcgaaggcg
     2641 cgcaagtgat tgcgagctct cccgaagaat acgacgccct cattcgccgc gacctcgcca
     2701 aatgggcgaa ggtgatcgcc gcagccggca tcaccccgca gtgaggtcgt cattccgttg
     2761 aaaaacggaa tcaagtctca ccgcg
//
LOCUS       DASURG010000037         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1439443, whole genome shotgun sequence.
ACCESSION   DASURG010000037 DASURG010000000
VERSION     DASURG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439443"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFY80_00595"
     CDS             <1..726
                     /locus_tag="VFY80_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6006425.1"
                     /translation="MHAVRSRGWRGAVVHFRGCSGEPNRLPRAYHSGDTDELEWILQR
                     LQAERAGAPLYAAGVSLGGNVLLKWLGERGAAAAPYVTRACAVSAPVDLMSAGAALGE
                     GFNLFYARHFLATLKRKSVRMLESYPGLFALERVLAARTLREFDDVVTAPLHGFRDTD
                     DYWTRASSKPLLRHIDVPTLMINARNDPFLPAHALPRPHEVSGRVTLDFPESGGHAGF
                     ITGRFPGRTDWLPRRMLHYFAGN"
     gene            769..2145
                     /locus_tag="VFY80_00600"
     CDS             769..2145
                     /locus_tag="VFY80_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX6006426.1"
                     /translation="MNTLPPEIFKAYDVRGIVGRTLTPHIVEAIGQAIGSEARARRCN
                     AVAIGRDGRLSGPELAAALARGLQKSGVDVIDVGQVATPMLYFAAYHLETHSGVSLTG
                     SHNPPDYNGLKMMVGGETLWGDGIQALRRRIERGDLAQGSGSYREQSIHDAYLQRITA
                     DVKLERPMSVVVDCGNGVAGGTAPELYRQLGCSVTELFCEVDGRFPNHHPDPSQPKNL
                     EDLRKALAAGKGEIGFAFDGDGDRLGVVTKRGKIIFPDRLLMLLAADVLKRAPGAQII
                     FDVKSTRNLFSWVRDHGGKPLLWKTGHSLIKQKLKETGAALAGEMSGHFFFKERWYGF
                     DDGLYAGARLLEILSGESDPGAVLESLPDAISTPELHIQLAEGENFTLVEKLRQTARF
                     EGAQDIITIDGVRVEYADGFGLARPSNTTPVVVLRFEADNESALQRIQEDFRRAIEAV
                     KPGVSLPF"
     gene            complement(2191..2664)
                     /locus_tag="VFY80_00605"
     CDS             complement(2191..2664)
                     /locus_tag="VFY80_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6006427.1"
                     /translation="MAVRPLLRAQDIAAMPEHARVHALDPGAIRHTRSLGDAAGLSTL
                     GVHLVRLTQGCRSSVLHFHHHDEEWIYVLSGRGVAELGDARHEVGAGDFMGFVAGSVP
                     HNLFNPNAEDLIYLVGGNRLPYDVCDYPHIRMRRYRTNGVNEYVSLDAITPRPQR"
     gene            complement(2685..>2981)
                     /locus_tag="VFY80_00610"
     CDS             complement(2685..>2981)
                     /locus_tag="VFY80_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX6006428.1"
                     /translation="IEGADVKALREAVDKLKGKLKTAAVVLATADGGKVSLIAGVTAD
                     MTAKVKAGELVNYVAQQVGGKGGGRADMAQAGGTDAAKLPQALASVRAWIEQRL"
BASE COUNT          509 a          988 c          949 g          535 t
ORIGIN      
        1 atgcatgcag tgcgctcgcg cgggtggcgc ggtgccgtcg tgcattttcg cggctgcagc
       61 ggcgagccca atcgcctgcc gcgcgcatac cattcggggg atacggacga actcgagtgg
      121 atcctgcagc gcctgcaggc cgagcgcgca ggcgcgcccc tttacgcagc cggcgtctca
      181 ctcggcggca acgtgctttt gaagtggctc ggcgagcgcg gcgcggccgc tgcgccctat
      241 gtcacgcgtg cgtgcgcggt gtccgcgccg gtcgatctta tgagcgcggg ggccgcgctg
      301 ggtgaggggt tcaacctctt ctacgcccgg cacttcctcg cgacgctcaa gcgtaaatcc
      361 gtccgcatgc tggaatcgta cccgggtctc ttcgcgctcg agcgcgtgct tgccgcacgg
      421 accctgcgcg aattcgatga cgttgtcacc gcgccgctgc acggctttcg cgacaccgac
      481 gattactgga cgcgcgcgag cagcaagccg ctgctgcgcc atatcgacgt gccgaccctc
      541 atgatcaatg cccgcaacga tcctttctta ccggcgcacg ccctgccgcg cccgcacgaa
      601 gtctcggggc gcgtcacgct cgattttccg gagtccggcg ggcatgccgg attcatcacc
      661 ggacggtttc ccggacgcac cgactggcta ccgcggcgca tgctgcacta cttcgcggga
      721 aactgaatca ggattaagct gtccactccc catcctaatg gcgcatcaat gaacacactt
      781 cctcccgaga ttttcaaggc gtacgacgtg cgcggcatcg ttggccgcac gcttacgccc
      841 cacatcgtcg aagcgatcgg acaggccatc ggttccgaag cgcgcgcccg ccggtgcaac
      901 gcggtcgcga tcggccgcga cgggcgcctc tccggtcccg aactcgccgc agcgcttgca
      961 cgcgggctgc agaagagcgg cgtcgatgtc atcgatgtcg gtcaggtggc gacgccgatg
     1021 ctctacttcg ccgcttacca tctcgagacc cactcgggcg tctcgctgac cggatctcac
     1081 aatccgcccg attacaacgg cctcaagatg atggtcgggg gcgagaccct gtggggcgac
     1141 ggcatacagg cgcttcgccg tcgcatcgag cgtggtgacc tcgcgcaggg atcgggcagc
     1201 taccgcgagc agtcgatcca cgatgcgtac ttgcagcgca tcaccgcaga cgtgaagctc
     1261 gaacgtccca tgtcggtcgt ggtggactgc ggaaatggcg tcgccggggg caccgcgccc
     1321 gagctctatc gccagctcgg ctgcagcgtg acggagcttt tctgcgaagt ggacgggcgg
     1381 tttcccaacc atcatcccga tccctctcag cccaagaatc tggaagacct gaggaaggcg
     1441 ctcgcggcgg gcaagggcga gatcggtttc gcgttcgacg gcgacggcga ccggctcggc
     1501 gtagtgacga agaggggaaa gatcatcttc ccggatcggc tgctcatgct cctcgccgcc
     1561 gatgtcttga agcgcgcgcc gggcgcgcaa atcatcttcg atgtcaaatc gacgcgcaat
     1621 cttttttcct gggtacgcga ccatggcggc aaaccgctcc tctggaaaac cggacactcg
     1681 ctcatcaagc agaaattgaa ggaaaccggc gccgcgctcg cgggtgaaat gagcgggcac
     1741 ttcttcttca aggagcggtg gtacggtttc gacgatgggc tctacgccgg tgcgcgattg
     1801 ctggagatcc tgtcgggcga aagcgatccc ggtgcagtgc tggagagcct gcctgacgcg
     1861 atctcgacgc cggagcttca cattcagctc gccgagggcg aaaacttcac gctcgtcgag
     1921 aagttgaggc aaacggcgcg tttcgaaggc gcgcaggaca tcatcacgat cgacggcgta
     1981 cgggtggaat atgccgacgg attcgggctc gcgcgtccct caaacacgac gccggtcgtc
     2041 gtgctgcgtt ttgaagccga taacgaatcg gcgttgcagc gcattcagga agacttccgc
     2101 cgtgcgatcg aggcggtgaa gccgggagtc agccttccct tctagatgag gcgccgccgg
     2161 agcggataac cgtttcgccg aagggaggac tcaccgctgc gggcggggcg tgatcgcgtc
     2221 caggctcacg tactcgttca cgccgttcgt gcggtagcgc cgcatgcgga tgtgcgggta
     2281 gtcgcagacg tcgtacggca gccggtttcc gcctaccaga taaatcagat cttcggcgtt
     2341 cgggttgaag agattgtgcg gaacggaacc ggcgacgaaa cccatgaagt cgcccgcgcc
     2401 cacttcgtgc cgtgcatcac cgagttccgc gacgccgcgc cccgagagca cgtagatcca
     2461 ctcctcgtcg tggtggtgga agtgcagcac gctgcttctg cagccttgcg taagccgcac
     2521 gaggtgcacg ccgagcgtgc tcaggcccgc ggcatcaccg agcgagcgcg tgtgccgtat
     2581 cgcaccggga tcgagcgcgt gtacgcgcgc atgctccggc atcgccgcga tgtcctgcgc
     2641 gcggagcagt ggacggacgg ccatgtgacc ctcctgaaat cgtatcagag ccgttgttca
     2701 atccacgccc gcacggatgc gagcgcttgg ggaagcttcg cggcatcggt gccgccggcc
     2761 tgtgccatgt ccgcgcggcc gccgcccttg ccgccgacct gctgcgcgac gtaattcacg
     2821 agctcgcccg ccttcacctt cgccgtcatg tcggccgtga cgccagcaat aagcgaaacc
     2881 tttcccccat ccgccgtcgc cagaacgacc gccgcagtct tgagcttgcc tttcagcttg
     2941 tccacggcct cgcgcagcgc tttcacatcc gcaccctcga t
//
LOCUS       DASURG010000038         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_316283, whole genome shotgun sequence.
ACCESSION   DASURG010000038 DASURG010000000
VERSION     DASURG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316283"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFY80_00615"
     CDS             <1..523
                     /locus_tag="VFY80_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6006429.1"
                     /translation="PIKGLRWAFSHLDQLTSAQIERMKRLGMSAQIHSRPLIQGALMH
                     KFHGERAWEMPPMRMVQDSGIMWGLGSDATAVTTSDPFFTLWFAVTGKMIGGRKVNRQ
                     TITREEALTAHTRNNAYIVFQESNLGSLQPGKYADLVVLDRDYLTVPEDDIKDLNPLM
                     TMVGGRIVYEAKK"
     gene            533..1765
                     /locus_tag="VFY80_00620"
     CDS             533..1765
                     /locus_tag="VFY80_00620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6006430.1"
                     /translation="MDVIMPQLGETVAEGTVTRWYKKVGEPVKADEALFDVETDKVST
                     EIPAPVNGVLAEIVVAEGVTAKVGARLAVIRESGVATASPAAPSAQPVATREGAGSPR
                     ATSAAPALRADASERLSPVVRKLCAEQGIDPKAIAGTGRDGRITREDVLEYIAARGAE
                     AQPAPAQHVPDAAAPGPIGLTNVRRRTAEHMARSWATAPHVMQAVEADFHRVVDARRK
                     AGEQWKAREGYALTYLPFIARAVSVALSKFPNLNATFDGTNLSLHRRINLGIAVDLNF
                     EGLVVPVVKDVPSKSLPQIAREINDLAARARAGRLKPDDLTEASYTITNNGAFGTLFT
                     APIINTPQVAILSTDAIRKKPVVIEAPDGDSIAIRPVGMLAQSFDHRAVDGAYAAAFL
                     QEVKAVIETRDWLQALQA"
     gene            1785..>2488
                     /locus_tag="VFY80_00625"
     CDS             1785..>2488
                     /locus_tag="VFY80_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6006431.1"
                     /translation="MAKAALVDPAWVHRHRNDPKVKLIEIAGMGQEEMQAYRAGHVPG
                     AVCWKWKEMLWDSHARDFPAPEEFARRCAAAGIGNDTTVVFYGEGVQFGVYAWWTFRY
                     CGHPKVRVLDGARYRWAAEGRPLVTEDPPPPTPAGYRPVKRKEAMRVHREKVLRALGR
                     EGLVILDARSPEEYRGERVGGPGGPDVGAVRYGRIPGAKHLYFEDLLDANKAFRPEAE
                     IRALVESRGATPENDV"
BASE COUNT          459 a          772 c          864 g          393 t
ORIGIN      
        1 gccgatcaag ggtttgcgct gggctttttc gcacctcgac cagttgacgt cggcgcagat
       61 cgagagaatg aagcggctcg gcatgtcggc acagattcac agccggccgc tgatccaggg
      121 tgccctcatg cacaagtttc acggcgagcg cgcttgggag atgccgccga tgcgcatggt
      181 gcaggacagc ggcatcatgt gggggctggg ctccgacgca acggccgtga ccacgtccga
      241 cccgttcttc accctgtggt tcgcagtgac ggggaagatg atcggaggcc gcaaggtgaa
      301 ccgccagacc atcacgcgcg aggaggcgct gaccgcgcat acgcgcaaca acgcctacat
      361 cgtcttccag gaaagcaatc tcgggagcct tcagcccggc aagtacgcgg atctcgtcgt
      421 gctcgaccgg gattacctca cggtcccgga agacgacatc aaggacctga atccgctcat
      481 gaccatggtg ggcggcagga tcgtgtatga ggcgaagaaa taggagaagc ggatggatgt
      541 catcatgccg caactcggcg agacggttgc cgagggcacc gtcacccgct ggtacaagaa
      601 agtgggcgag ccggtgaagg cggatgaagc gctgttcgat gtcgaaacgg acaaggtcag
      661 caccgagata ccggcgccgg tgaatggcgt gctggccgag atcgtcgttg cggagggcgt
      721 tacggccaaa gtgggtgcgc ggctcgccgt gatccgcgaa agcggtgtgg cgacggcctc
      781 gcccgccgca ccgagcgcgc agcccgtcgc gacacgagag ggggccggca gtccgcgcgc
      841 tacgtccgcg gcaccggccc tgcgagccga cgcatcggag cggctctcgc cggtggtgcg
      901 gaagctgtgc gccgagcagg gtatcgatcc caaagcgatc gccgggaccg gccgggacgg
      961 acgcatcacg cgcgaggatg tcctggagta catcgcggcc cgtggcgcgg aggcacagcc
     1021 tgccccggcc cagcatgtcc ccgacgcggc tgcccctggt cccatcgggc tcaccaatgt
     1081 gcgccgccgc accgccgagc acatggcgcg ttcgtgggcg actgcgccgc acgtcatgca
     1141 ggcggtggaa gcggatttcc atcgcgtcgt cgatgcgcgg cgcaaggcgg gcgagcagtg
     1201 gaaagcgcgg gagggttatg cgctcacgta tctgcccttc attgcacgtg ccgtatcggt
     1261 cgcgctatcg aaattcccca atctcaatgc gacgttcgac ggcacgaatc tttcactgca
     1321 ccgcagaatc aatctcggca tcgcggtcga tctcaacttc gaggggctcg tcgtgcctgt
     1381 ggtgaaggac gtgccttcca aatcgctgcc gcagatcgca cgcgagatca acgatctcgc
     1441 agcgcgggca cgcgcggggc ggttgaaacc tgacgatctc acggaggcga gctacaccat
     1501 caccaataat ggcgcgtttg gcacgctctt caccgcgccg atcatcaaca ccccgcaggt
     1561 cgcgatcctt tcgaccgacg cgattcgaaa gaagccggtc gtcatcgaag cacctgatgg
     1621 cgacagcatc gcgatccgcc cggtcggcat gctggcacag agcttcgatc accgcgcggt
     1681 ggacggcgcc tatgccgccg ctttcctgca ggaagtgaag gcggtcatcg agacacgcga
     1741 ctggctgcag gcgctgcagg cctgaaggcc gaaggaggga cacgatggca aaagcggcat
     1801 tggtcgatcc ggcatgggtt caccggcacc ggaacgatcc caaggtgaag ctgatcgaga
     1861 tcgccggcat gggccaggaa gagatgcagg cctacagggc cggccacgtg ccgggtgccg
     1921 tgtgctggaa gtggaaggag atgctgtggg attcccacgc gcgggatttt cctgctccgg
     1981 aggaattcgc gcgccgctgc gctgctgcgg gtattggcaa tgacaccacc gtcgtgtttt
     2041 acggcgaggg cgtgcagttc ggcgtgtacg cgtggtggac tttccgctac tgcgggcatc
     2101 cgaaagtgag ggtgctcgat ggcgcccgct atcgctgggc ggcggaaggg cggccgctcg
     2161 tcactgagga tcccccgccc ccgacgccgg ccggttaccg gccggtcaag cgcaaggaag
     2221 cgatgcgcgt ccatcgcgag aaggtgctgc gcgccttggg ccgcgaaggg ctcgtcatac
     2281 tcgatgcccg ctcccccgag gaataccgcg gcgagcgggt cggcgggccg ggcgggcccg
     2341 acgtcggcgc cgtgcgttat gggcgcatcc cgggcgcgaa gcatctctac ttcgaggacc
     2401 tcctcgacgc caacaaggcg ttcaggccgg aagcggagat ccgggcgctc gtcgaatcgc
     2461 gtggtgcgac gccggagaac gacgtgat
//
LOCUS       DASURG010000039         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_520331, whole genome shotgun sequence.
ACCESSION   DASURG010000039 DASURG010000000
VERSION     DASURG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520331"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFY80_00630"
     CDS             complement(<1..575)
                     /locus_tag="VFY80_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6006432.1"
                     /translation="MLKVAGAIARTAAVLLPGLLFSQAQAQSPRVPYVPTPQEVVERM
                     LEIAKVSASDYLIDLGSGDGRIVVTAAKKYGTRGFGVDINPERISEANENAKSAGVTD
                     KVAFYQRDLFETDLSEATVITMYLLPRVNLELRPRLLDLRPGTRIVSHDFSMEDWKPD
                     VHVKMEAKEKYGGSGGQSDIYFWVVPAKVNGA"
     gene            721..2316
                     /locus_tag="VFY80_00635"
     CDS             721..2316
                     /locus_tag="VFY80_00635"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6006433.1"
                     /translation="MLYTVIFISGGAILALELLASRIMTPYFGVSLYIWTGILSITLV
                     SLALGYWAGGRFAGGERGRPVTDLTQRYAMMPAVAALSIVGACLAYPHLFSPLAGWSL
                     ILGAFAACLILLFLPLVATSAMNPLLVAIALQNRARHSGDAGAGRVFFVSTIGSVAGV
                     LVTAFVMTPYLTNFAAVLVVAFTLALLSLAVAMTPSIRLSSRPAVVALSLAGASASLL
                     LLWQADAYTGRLQAASYAGRTWQVETSVNSLFGTVKILKSRPEDNGEFLRIYFQDGLT
                     QNTVDSNGRSMSFYTYALEALARAYRPDLQSALVLGLGAGIVPMQLARSGIAVDVVEI
                     DPASLYVAQHYFGFDPARAHVHQADARAFVRGCPRRYDVVIVDLFHGDGTPDYLVTRD
                     FFRDLKRCLADDGVAVFNTFADLDRPAAYAHLLATLHSELPAIALYRPDWRAVQLNSF
                     LVAARGALPRPAKVTLDWVPAHHASTLWSMLASPRPVDEKATQAGRVITDALNAVALD
                     FAAAQIAFRRSVVEAMPPALLLN"
     gene            complement(2318..>2978)
                     /locus_tag="VFY80_00640"
     CDS             complement(2318..>2978)
                     /locus_tag="VFY80_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006434.1"
                     /translation="LTAAKQFRARGLGIDIDPQLVKLSNDSAQRMGVADRASFQVQDV
                     FKADLSKASVLTLYLLPSMMGELQQKVYSELKPGARVVSHDYHFGDDWRPDDYVSFDV
                     PEKEKVNGVPRATVYLWVVPARIGGKWRLRIDGSAPEYEIEFKQRFQHFDGAAHAGGK
                     TLKLELPQLRGEDIRFAFSAGAGRHVFYGRVKGDAMEGKVQLGSGKGTARWTAARAGT
                     P"
BASE COUNT          459 a         1026 c          890 g          603 t
ORIGIN      
        1 gcgccgttca ccttcgcggg aacgacccag aagtagatgt cactctggcc gcccgagccg
       61 ccgtactttt ccttcgcttc catcttgacg tgcacgtcgg gcttccagtc ttccatcgaa
      121 aagtcgtgcg agacgatacg cgtgccgggc ctcaaatcga ggagccgggg ccgcagttcg
      181 agattgaccc gtggcagcag atacatcgtg atgacggtcg cttcactcaa gtcggtctcg
      241 aagaggtccc gctggtagaa cgccaccttg tctgtcacgc cggcgctctt cgcgttctcg
      301 ttcgcttcgc tgatgcgttc agggttgatg tctacgccga aaccgcgcgt gccatacttc
      361 ttcgccgcgg tgaccacgat ccggccgtcg cccgacccca ggtcgatcag ataatcgctt
      421 gcggatacct tcgcgatttc gagcatgcgc tcgacgacct cctgcggcgt aggcacgtag
      481 ggcacgcgtg gggactgcgc ctgcgcctgg gaaaacaaca aaccgggaag caaaaccgct
      541 gcggtgcgcg cgattgcgcc tgcgaccttc agcatgtgct gcacgtgcgc actccttttt
      601 ccagatccgt cctgctcctc ggagcgctgc ccggaagcct cgggcacaac gttcttatga
      661 tagattcgcg ggcacgccag acggcgcccc gttaaagcgc ggcattatac ccgaacaatc
      721 atgctctaca ccgtcatctt catctccggc ggcgcgatcc tcgcgctcga gctgctcgcc
      781 tcgcgcatca tgacgcccta cttcggcgtg agtctctaca tctggaccgg catcctgtcg
      841 atcaccctcg tatcgctggc cctcggatac tgggccggcg gccggtttgc cggcggcgag
      901 cgcggccggc cggtgaccga cctcacgcag cgctatgcca tgatgccggc ggttgccgcg
      961 ctctcgattg tcggcgcctg ccttgcctat ccccatcttt tctcgccgct cgcaggctgg
     1021 agcctcatac tcggcgcctt cgcagcctgc ctgatcctgc tctttctgcc actcgtcgcc
     1081 acttccgcga tgaatccgct gctcgtagcg atcgcgcttc agaatcgcgc gcggcacagc
     1141 ggcgacgccg gcgccggccg cgtcttcttc gtgagcacca tcggctcggt cgcgggcgta
     1201 ttggtcaccg ctttcgtgat gacgccgtat ctcaccaatt tcgcagccgt gctggtggtt
     1261 gccttcacgc tggcgctgct ttcccttgcc gttgcgatga cgccctcgat ccgtctcagc
     1321 tcgcggccag ctgtcgtcgc gctatcgctc gcgggcgctt cagcgtcgct tctcctgctc
     1381 tggcaggccg atgcctatac cggacggctt caggccgcct cctatgcggg ccgaacctgg
     1441 caggtcgaga cgagcgtgaa ttcgctgttc ggaacggtga agatcctgaa gagccgaccc
     1501 gaggacaacg gagaattcct ccgtatctat ttccaggacg gacttacgca gaacacggtg
     1561 gattccaacg ggcgttcgat gtcgttctac acctacgcgc tcgaagcgct ggcgcgcgct
     1621 taccgcccgg atttgcagag cgcactcgtg ctcggtctgg gcgccggtat cgttcccatg
     1681 cagcttgcgc gcagcggcat cgccgtggac gtcgtggaaa tcgatccggc atcgctctac
     1741 gtagcgcagc actacttcgg cttcgatccc gctcgcgcgc acgtgcacca ggccgatgcc
     1801 cgcgccttcg tgaggggctg cccgcgccgc tacgacgtcg tcatcgtcga tctctttcat
     1861 ggcgatggca cgcccgacta tctcgtaacg cgagatttct tccgggactt gaagcgctgt
     1921 ctcgccgatg acggtgtcgc ggtattcaat actttcgcgg atctcgatcg gcccgcagcc
     1981 tacgcgcatc tgctcgccac gctgcattcg gagctgccgg ccatcgcgct ctatcggccg
     2041 gactggcgtg ccgtgcagtt gaacagtttt ctcgtcgctg cgcgcggcgc gctgccacgt
     2101 cctgccaagg tgacgctcga ctgggtgccg gcgcatcacg cgagcacgct gtggagcatg
     2161 cttgccagcc cacgcccggt ggacgaaaaa gcgacacagg cgggccgcgt cataaccgac
     2221 gcgctcaacg ctgtcgcgct cgattttgcc gcggcgcaga tcgccttccg gcgttcggtc
     2281 gtggaggcga tgccgcccgc gctgctactg aattagctca gggggtgccg gcgcgggcgg
     2341 cggtccagcg cgccgtgccc ttgcccgagc cgagctgcac cttgccttcc attgcgtcgc
     2401 ccttcacgcg tccgtagaag acgtgccggc cagcgcccgc gctgaacgca aagcggatgt
     2461 cctcgccacg caactgtggc agctcgagct tgagcgtctt cccgcccgca tgtgccgcgc
     2521 cgtcgaagtg ctggaagcgc tgcttgaact cgatctcgta ctctggcgca gagccgtcga
     2581 tcctgaggcg ccattttccg ccgatccggg caggcacgac ccacagatat acggtcgcgc
     2641 gcggcacgcc gttcaccttt tccttttcgg gcacgtcgaa gctcacgtaa tcgtccgggc
     2701 gccagtcatc cccaaagtga tagtcgtgcg ataccacgcg cgcaccgggc ttaagctcgc
     2761 tgtacacctt ctgctgcagc tcgcccatca tggaaggcag cagatagagg gtgagcacgg
     2821 aagccttgct caaatccgcc ttgaagacgt cctggacctg aaaggaggcg cggtccgcca
     2881 cgcccatgcg ctgcgccgag tcgttgctca gcttcacgag ctgcggatcg atgtcgatgc
     2941 ccagcccgcg cgcccggaac tgcttcgcgg cggtgagc
//
LOCUS       DASURG010000040         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_112161, whole genome shotgun sequence.
ACCESSION   DASURG010000040 DASURG010000000
VERSION     DASURG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112161"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFY80_00645"
     CDS             <1..775
                     /locus_tag="VFY80_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006435.1"
                     /translation="FRGDLVASIRESLARYRIEYIPGAADRGDYDPTSTTIALSIGDM
                     QSDLPQHALHATFERYWAEFLTRRNRANWNDYTPYELRNVGAFARLGWRDRIDPLLEF
                     FHGDRRPRAWNQWAEVVGREPRTPRFIGDMPHGWVASDYINAVLDLFAYERRADRALV
                     LAAGIPGPWLEGEGIGITHLRTPYGALTYRLRRQAGELMLEADGTAMPPGGFVLPWPY
                     AGRPGRAMLDGQALHWEGDRELRIRTLPARVRIEVPGDP"
     gene            841..1704
                     /locus_tag="VFY80_00650"
     CDS             841..1704
                     /locus_tag="VFY80_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6006436.1"
                     /translation="MNFSEQRKKLRAVLGGTKCVSPASVFDPLSARVAESVGYEIGML
                     AGSVASNTTLAAPDLIVLTLTELADQIRRIMRVSRLGLFVDADHGYGNALNVMRTVQE
                     LEHAGVSGMSIEDTALPLRFGQPEGADELISTDEMIGKLRAAVTIRTDPSLVIAGRTA
                     ALKAEGTEAAVARAKAYAATGVDAIFVVGVENLDQVAAVHDAARVPIIVGSAPGSLKR
                     EDFAARGARILLQGHQPVAAAVKALHETYTHLYNGGAPADLKSRIASAQEMERLVFGA
                     DYRTWQREFLR"
     gene            1730..2659
                     /locus_tag="VFY80_00655"
     CDS             1730..2659
                     /locus_tag="VFY80_00655"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6006437.1"
                     /translation="MKKVSSPSHTSVGIIGLGIMGGAMAQALCAAGYPVCGYDVALPA
                     RRRLRKSGGLPLDSSTAVAARADVVITSLATVAALEDVVGKIAGVPREKVRTRVLIET
                     STLPLGDKVLAMERLRRNGIATLDCPISGTAVRLKQGAWTIFASGSRAAFRRILPILG
                     VFTRNVPYVGAFGNGTRMKFVANHLVAIYNVAAAESLVFARKMGLDPQQVLDLFGPSP
                     VVGNGVLRLRGAQMVARKYEPATMKVEVWQKDMRVIGDMARAVDCPVPLFAGCMPIYN
                     AAMAQGLAHADTASVHEVLSRMAGVGAARPRRA"
     gene            2666..3430
                     /locus_tag="VFY80_00660"
     CDS             2666..3430
                     /locus_tag="VFY80_00660"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6006438.1"
                     /translation="MAHSTTMRQVAFVSGASQGLGAAIALALARDGFDVAVSSTNAAN
                     LAQTVKGIEAAGRRAFAVALNLSEVLGVERAIAAAVAALGPLELLVNNAGITTRKSAL
                     EMTPDEWDPILAVNLKGAFFLTQQMGRHLVASGRPGAIVNLASTHGIVAFPQRLAYGV
                     SKAGLIHMTKMLAVEWAPYGIRVNAIAPGTVSTPSREAHFSSDPAARETMVARVPLKR
                     FATMEEVGAAVCYLASPAARYVTGHTLVLDGGLTAY"
     gene            3476..4447
                     /locus_tag="VFY80_00665"
     CDS             3476..4447
                     /locus_tag="VFY80_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006439.1"
                     /translation="MKILFCCWTLVAASIGCAAPALAQAPYPAKPVRLIVPYPPGGGT
                     DTMARTLALKLAETLGQQVVVDNRPGGGANIGAEIAAKSPADGYTLLMCTIAHATSGS
                     LYRKLGYDVLRDFAPVSLLATTPHILVTHPSVPVKSVKELMLLAKARPNELVYSSSGS
                     GTPAHLAGELFKHMAGVNMTHVPYKGGGPSVVALLSGEVMLAFATTPSVINHVKAGRL
                     RAIAVTTARRSVATPDLPTISELGLKGYDVGSWYGLLAPAGIPKDVVVRLNAEVHRVM
                     RLPDVKQRLDAGGFEAWTSTPEEYGVFLRGEVEKWAKIIKAAGIRAD"
     gene            4477..>4775
                     /locus_tag="VFY80_00670"
     CDS             4477..>4775
                     /locus_tag="VFY80_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006440.1"
                     /translation="MSAVRRTALWASIGLALMASATAWGQSYPARPVRVIVGFGAGAP
                     DTVARIVTQQLAAQMGQPFVVDNRPGANGIIGAELVVKATPDGYTLLVTSASFAV"
BASE COUNT          829 a         1487 c         1615 g          844 t
ORIGIN      
        1 atttcgtggc gacctggtag cctcgatacg cgagagtctc gcacggtacc ggatcgaata
       61 cattccgggt gctgccgatc gcggcgatta tgacccgact tcgaccacca tcgcgctttc
      121 gatcggcgac atgcaatcgg acctgccgca gcacgcgttg catgcgacgt tcgagcgcta
      181 ctgggcggaa ttcctcacac gccgcaatcg cgcaaactgg aacgactaca caccgtacga
      241 actgcgcaac gtcggtgcgt tcgcccggct cggctggcgg gaccgcatcg atccgctgct
      301 cgaatttttc cacggcgatc gccggccgcg tgcatggaat cagtgggcag aggttgtcgg
      361 gcgggagccg cgcacgccgc gctttatcgg cgacatgccg cacgggtggg tcgcgtcaga
      421 ctatatcaac gctgttctcg atctcttcgc ctatgagcgg cgcgcggatc gtgcactcgt
      481 tctcgccgcc ggcattccgg gaccctggct cgaaggggag gggatcggta tcacgcatct
      541 gcggacgccc tatggcgcgc tcacttaccg gctgcggcgg caagccggcg agctgatgct
      601 cgaagcggac ggcacggcga tgcccccggg tgggttcgta ctcccatggc cgtatgccgg
      661 gcgcccgggt cgggcgatgc tcgacggaca ggcgcttcac tgggagggcg accgcgagct
      721 tcgcatccgt acgctgcctg cgcgcgtgcg catcgaagtc ccgggcgatc cgtgaggcga
      781 cgctgcgcgc gtgctggtat gatcgcggcc ggaccgcgat caatggagag ctcgggaatc
      841 atgaatttca gcgaacagcg taaaaagcta cgcgcggtac tgggaggcac gaaatgcgtt
      901 tcgccggcga gcgtgttcga tccgctttcg gcgcgtgtcg cggaaagcgt cggctatgaa
      961 atcggcatgc tggccggctc ggttgcttcg aacacgaccc ttgccgcacc ggatctgatt
     1021 gtcctcacgt taaccgagct tgcagatcag atccggcgca tcatgcgggt cagcaggctt
     1081 ggtcttttcg tggatgcgga tcatggctat ggcaatgcgt tgaacgttat gcgcaccgtg
     1141 caggagctcg agcacgccgg cgtgtcgggc atgagtatcg aagacaccgc gctccccctg
     1201 cgatttggcc aacccgaagg cgcggatgaa ttgatctcca ccgacgagat gatcgggaag
     1261 ctccgcgcgg cggtcacgat ccggaccgat ccctcactcg tcattgcggg ccgcacggca
     1321 gcgctcaaag cggaaggcac ggaagccgcg gtcgcgcgcg caaaagcgta cgccgctaca
     1381 ggggtggacg cgatctttgt cgtgggggtc gaaaacctcg atcaggtcgc agcggttcac
     1441 gatgcggcgc gcgtgcccat catcgtgggg tctgcgccgg gatcgctcaa acgcgaagac
     1501 ttcgccgcgc gcggcgcgcg cattctgctt caggggcatc aaccggttgc cgcagcggtg
     1561 aaggcgctgc acgagaccta tacgcatctt tacaacggag gggcgccggc ggatctcaag
     1621 tcgcgcatcg cgagcgcgca ggagatggag cgcctcgtgt tcggcgcgga ctaccgcacc
     1681 tggcagcgcg agtttctgcg ctgacgcccg aagttcgctg gactgcccgg tgaagaaggt
     1741 ctcgtctccg tcacatacga gcgtcggcat catcggcctc ggtatcatgg gcggtgccat
     1801 ggcgcaggcg ctttgtgcgg cgggataccc ggtgtgcggc tatgacgtcg cgctaccagc
     1861 gcggcgacgg ttgaggaaat ccggcggtct cccgttggat tcgagcacgg ccgttgcggc
     1921 gcgggccgat gtggtgatca cttcgctcgc cactgttgcg gcgctcgaag acgtagtcgg
     1981 aaaaatcgcc ggcgtcccgc gcgagaaagt ccgaacgcgg gtgctcatcg agacgagcac
     2041 tttgccgctc ggcgacaagg tgctcgcgat ggagcgcttg cgccggaacg gcatcgctac
     2101 gctcgattgc cccatcagcg gcaccgcggt acggctgaaa caaggcgcgt ggaccatttt
     2161 cgcgagcggc agccgggcag cattccggcg cattttgccg atcctcggcg tattcacccg
     2221 caatgttccg tatgtcgggg cattcggcaa cggcacgagg atgaagttcg ttgctaacca
     2281 ccttgtcgcc atctacaacg tggccgcggc agagtctttg gtttttgcgc gcaagatggg
     2341 actggacccg cagcaggttc tcgacctgtt cggcccaagt ccagtcgtgg gcaacggcgt
     2401 gctgcggctg cggggcgcgc agatggtcgc gcgtaagtat gagcccgcga cgatgaaggt
     2461 cgaagtctgg cagaaggaca tgcgagtcat aggtgacatg gcgcgagcgg tcgattgtcc
     2521 cgtgccgctc ttcgcaggct gcatgccgat ctacaatgcg gcgatggcac agggccttgc
     2581 gcacgcggac accgcctcgg tacacgaagt actctcacgc atggcgggag tcggcgccgc
     2641 cagaccccgc agggcctgag tcagcatggc gcattcgacc acgatgcggc aagtcgcctt
     2701 tgtctctggc gcatcacaag gcctcggcgc ggcgattgcg cttgcgcttg cgcgggacgg
     2761 tttcgacgtg gcagtgtcga gcaccaacgc ggcgaatctc gcgcagaccg taaagggtat
     2821 tgaagccgcc ggccggcgcg cgtttgccgt tgctctcaac ttgtcggaag tattgggtgt
     2881 cgagcgcgcg atcgcggccg cagtcgcggc actggggccg cttgagctcc tcgtcaacaa
     2941 cgccggcatc acgacacgca aatccgcact cgaaatgact cccgacgagt gggatcccat
     3001 ccttgcggtc aatctgaagg gcgccttctt tctgacccag cagatggggc gccacctcgt
     3061 cgcatccggc aggccgggcg ccatcgtcaa cctcgcgtcg acgcacggca tcgtcgcctt
     3121 tccgcagagg ctggcgtacg gggtatcgaa ggcgggcctc atccatatga cgaaaatgct
     3181 cgcggtcgag tgggcgccat acgggatacg tgtgaacgcg attgcgcccg gcacggtgag
     3241 tacgccctcg cgcgaggcgc atttctcgag cgatccagca gcgcgcgaga cgatggtcgc
     3301 gcgggtgccg ctcaagcgat tcgcgacaat ggaggaagtc ggggccgcgg tgtgctatct
     3361 cgcgagccct gcagcccgat atgtaaccgg tcatacactc gtgctcgacg gcggactgac
     3421 tgcgtactga agagcggtgc ggggctatgg cacgagagag aaaggaggga gtgcgatgaa
     3481 gatactgttc tgctgttgga cgctcgtcgc ggcatcaatc ggctgcgccg ccccggcgct
     3541 cgcccaggcg ccatatcctg ccaagcccgt ccggctcatc gtgccttacc cgccgggcgg
     3601 cggtaccgac accatggcgc gcacgctggc gctcaaactg gcggagacgc tggggcagca
     3661 ggtggttgtg gacaaccggc cgggcggcgg agcgaacatc ggcgcggaga tcgcggccaa
     3721 gtcgccggcc gacggctata cgctgctgat gtgcaccatc gcgcacgcca cctcgggcag
     3781 cctctatcgc aagctcggat acgacgtgct gcgcgatttc gccccggtgt cgctgctcgc
     3841 cacgaccccg cacatactcg tgacccatcc gtcggtgccg gtgaaatcag tcaaagagct
     3901 gatgcttctc gcgaaggcgc ggccgaacga gctcgtgtac tcttcctccg gcagcggcac
     3961 gcccgcccat cttgccggcg agcttttcaa gcacatggcc ggggtaaaca tgacccatgt
     4021 tccctacaaa ggcggcggcc cttcggtcgt cgcattgctg agcggcgaag tgatgctcgc
     4081 cttcgcgacc accccgtcgg tgatcaacca cgtgaaggca ggccgactgc gcgctattgc
     4141 agtcaccacg gcacggcgct cggtggcgac accggatctg cccacgatca gcgagctggg
     4201 tctcaagggc tacgacgtgg gatcgtggta cgggctcctg gcgccggccg gtatcccgaa
     4261 ggacgtcgtc gtgcgactga acgccgaggt tcacagagtg atgaggcttc ccgatgtgaa
     4321 gcagcgcctc gacgccgggg ggttcgaagc gtggacgagc acgcctgagg agtatggggt
     4381 tttcctgcgc ggggaagtgg aaaaatgggc gaagatcatc aaggcggcgg gcattcgcgc
     4441 ggactgaggc cgtcttatca tccggagcat tgcagcatga gcgcggtgcg gcgaacagca
     4501 ctgtgggcat cgatcgggct cgcgcttatg gcgagcgcaa ccgcgtgggg ccagtcctat
     4561 cccgcgcgtc cggtgcgcgt catcgtaggt ttcggtgccg gcgcccccga tacggtggca
     4621 cgcatcgtga cgcagcagct cgcggcgcag atgggacagc cgttcgtcgt ggacaaccgg
     4681 ccgggcgcca acggcatcat cggggcggaa ttggtcgtca aggctacacc ggacggctac
     4741 accttgctcg tgacctcggc gtccttcgcc gtgaa
//
LOCUS       DASURG010000041         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_418332, whole genome shotgun sequence.
ACCESSION   DASURG010000041 DASURG010000000
VERSION     DASURG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418332"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFY80_00675"
     CDS             <1..500
                     /locus_tag="VFY80_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX6006441.1"
                     /translation="TTGVGIEKKLDEFEKAHDDYSSIMLKALADRLAEAFTELLHKRV
                     RKEFWAYAPDESLDNAALIKEQYRGIRPAPGYPACPDHTAKGPLFDMLQCERVGIRVT
                     ESYAMQPASSVSGFYFSHPESRYFAVGKVGRDQIADYARRKGVPVAEAERWLAANLGY
                     EPARG"
     gene            620..1471
                     /locus_tag="VFY80_00680"
     CDS             620..1471
                     /locus_tag="VFY80_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6006442.1"
                     /translation="MGGERYLALVREGRNEGWRYVLSVVLIISCWLVLGYLPYYALAA
                     HGPLNRLTLYIAVNCSIFAMLVGLALAMRFLHRRPLMTLVSAEQRFDWKRIVQAAAVW
                     AAIGLTAAAIEHLLYPDRYYVTFDPGAFFVFAAIALVLTPIQTTAEELLFRGYAMQAL
                     SLVVRRPLAIAAVSALVFTLPHLLNPEVRQAGMLVAASYYTIGFLLAVITLRDGRLDL
                     ALGVHAANNLGLALIANYEGSALTGESIFTARELDPVYTFFSLAAGSVLFYAFFFARR
                     STAAIKT"
     gene            1478..>2210
                     /locus_tag="VFY80_00685"
     CDS             1478..>2210
                     /locus_tag="VFY80_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX6006443.1"
                     /translation="MGASAFSAAELAQFAHDGYVIARRLAPPETLHRMLELARAYLAA
                     EAAPLEYEADLHYPGAPASRDAPGGRTVRRLLQAYARDPLFRDWATSADLRRRLEPLL
                     GGPVELAQAHHNCIMTKGPRYSSVTGWHQDIRYWAFDRPELVSVWLALGSERRENGCL
                     LVLPGSHRMDFEAHRLDAAKFLRSDLEENGVLLRTELAVELDPGDALFFHCRLFHAAG
                     ANRTDATKYSAVFTYHAADNRPVPGT"
BASE COUNT          342 a          747 c          705 g          416 t
ORIGIN      
        1 tgacgacggg cgtcggaatc gagaagaagc tagacgagtt cgagaaggcg cacgacgact
       61 attcgtcgat catgctgaag gcgctcgcgg accgtctcgc ggaggcattc acggagctac
      121 tgcacaagcg cgttcggaag gagttctggg cctacgcgcc cgacgagagc ctcgacaatg
      181 ctgcgcttat caaggagcag tatcgcggca tccgacctgc gcccggctac ccggcgtgcc
      241 ccgatcacac cgccaagggc ccgcttttcg acatgctcca gtgcgagcgg gtcgggatac
      301 gcgtgaccga aagctacgcg atgcagccgg cctcgtccgt gagcggcttc tacttttccc
      361 atcccgagtc gcgttatttc gcggtgggga aggtcgggcg cgaccagatc gccgactatg
      421 cgcgccgcaa aggcgttccg gttgcggaag cggagcgctg gcttgccgcg aatctcggtt
      481 atgagccggc gcgcggataa gcgcgctatg ccgcccatcg cttgcttcaa ccctccctca
      541 ggtggggccg gggcgaggct ttgtcgggcg accgctcggc ctcagagcat taaagcgcaa
      601 cgcgggcgtg ttcccgggca tgggcggtga acgttatctc gcgctcgtgc gcgagggacg
      661 caacgaagga tggcgctacg tcctatcggt cgttctcatc atttcgtgct ggctcgtgct
      721 cggatatctg ccgtactacg cgcttgcggc gcacggcccg ctcaaccggc tcaccctcta
      781 catcgcggtc aactgcagca ttttcgcgat gctcgtcggt ctcgcgctcg ccatgcgctt
      841 cttgcaccgg cgccccctca tgacgctcgt cagtgcggag cagcgtttcg actggaagcg
      901 catcgtgcag gctgcggcgg tatgggctgc catcggcctt accgccgcag cgatcgagca
      961 cctgctgtac ccggatcgct actacgtcac cttcgatccc ggagcgttct tcgtctttgc
     1021 ggcgatcgcg ctggtgctga cgccgatcca gacgacggcc gaggagttgc tctttcgcgg
     1081 ttatgccatg caggcgctgt cgctcgtcgt gcgcagaccg ctcgcgatcg ctgcggtgag
     1141 cgccctcgtc ttcacgctgc cgcatctgct caatccggaa gtgcggcagg cgggaatgct
     1201 cgttgccgcc agctattaca cgatcggctt tctgctcgcc gtcatcactc tgcgcgatgg
     1261 gcggctcgac ctcgccctcg gcgtccacgc ggcgaacaac ctcgggctcg cgctcatcgc
     1321 gaattacgag ggctccgcgc tgaccggtga gtcgatcttc accgcgcgcg agctcgatcc
     1381 cgtctatacg tttttttccc ttgcagccgg ctcggtgctg ttctacgcgt ttttcttcgc
     1441 gcgccgtagc accgcggcga ttaagaccta agagcccatg ggcgcttctg ccttcagtgc
     1501 tgcggaactc gcacaattcg cgcacgacgg ctatgtgatc gcgcggcgtc tggcgccgcc
     1561 cgaaacgctg catcgcatgc tggaacttgc gcgcgcgtat ctggcagccg aagcggcgcc
     1621 gctggagtac gaagcggatc tgcactaccc gggcgcaccc gcctcgcgcg atgcaccggg
     1681 cggacgcacg gtgcgccgac tgctgcaggc gtatgcgcgc gacccgctat tccgcgactg
     1741 ggcgacgtcg gcagacctgc ggcggcgact cgagccgctt ctcggcggcc cggtcgagct
     1801 cgcgcaggcg caccacaatt gcatcatgac caaagggccg cgctacagca gcgtgaccgg
     1861 ctggcaccag gacattcgct actgggcgtt cgaccggccg gaactcgtct cggtatggct
     1921 ggcgctgggc tccgagcgtc gcgaaaacgg gtgcctgctt gttctacccg gcagccaccg
     1981 aatggacttc gaggcgcatc gtctcgatgc cgccaagttt ctccgcagcg atctcgagga
     2041 gaacggggtg ctgctgcgca cggaactcgc cgtggagctc gacccgggtg acgcgctgtt
     2101 cttccactgc cggctcttcc acgccgcggg cgcgaaccgg acggatgcga cgaagtactc
     2161 cgccgtcttc acctaccacg ccgccgacaa tcgccccgtg ccgggcacgc
//
LOCUS       DASURG010000042         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_214099, whole genome shotgun sequence.
ACCESSION   DASURG010000042 DASURG010000000
VERSION     DASURG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214099"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFY80_00690"
     CDS             complement(<1..663)
                     /locus_tag="VFY80_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6006444.1"
                     /translation="MSTTDVISPDLRTVLRRLKLSPILTTLPERLTLARQQQIAHQDF
                     LLLVLADEAARRDSLATSLRVQRARLDPAMQLELWDTTARVTYDQALWQELVSLRFLE
                     QHKHLIIAGPVGVGKTFLAHALGHIACRHGHSVLAVRADKMLKTLKHARLDNSYEAEL
                     RKFLAVDLLIVDDFALDSMDAVESRDAHEIITERHRAGAMIFTSNRGPDEWLTMFADP
                     MRA"
     gene            complement(678..2240)
                     /gene="istA"
                     /locus_tag="VFY80_00695"
     CDS             complement(678..2240)
                     /gene="istA"
                     /locus_tag="VFY80_00695"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX6006445.1"
                     /translation="MAYREVTSVETKEVLRQWLGGARHKRIATRLGLDVKTVRRYVRA
                     AQVAGLTRTSGESALTDELLTAMLASLRTTQPGRPRGEAWERCEAHRERIAQWLAQGL
                     RLSKLRKLLLRHGVQVPYATLHRFAVAELGFGRSAATVPIADCAPGEELQVDTGWMGS
                     LEPDVFGKRRRFRAWIFTAVYSRHRFVWPCLHETTASAIEACEQAWQFFGGVFRVLLP
                     DNTKAIVTQADPLGARINETFLEYAQARGFVVDPARARHARDKGRVERAVPSVRDDCF
                     AGETLHAITEARGLAERWCRQEYGLRRHTRTQRLPLECFEAEEQPHLLPAPALPYDVP
                     HWCDPKVGRDHLAQVAKALYSLPTRFIGQHLRARADRALVRFYDQGMLVKTHPRQPPG
                     GRSIDANDFPAHKSAYAMRDIAFLERQAFQHGEAIGRFAHQVLEGPLPWTRMRRVYAL
                     LGLVRRYGAARVEPTCSVALDAQMHDVRRLDRMLQAASVAPTTPPPTTTAPARHLRPA
                     SQYALPFTTREH"
     gene            complement(2483..3178)
                     /locus_tag="VFY80_00700"
     CDS             complement(2483..3178)
                     /locus_tag="VFY80_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6006446.1"
                     /translation="MVAVVLLPGMDGSGVLFDEFASELDCRSIVISYPTDRPFGYEEL
                     EHHVRSLLPADESLILLAESFSGPLAIALAANPPPQLKALVLVCTFARFPVPSFALPC
                     FKALGLLPLWRAPMSLASRMLFGRFRSEAKEAVLSRAIRAVTPQVWRAGLKAVLSIDK
                     TSSLCHIRVPLLYLRASEDRVVFSSASAVISARVPGTKVVSIEGPHFLLQSKPQECAA
                     AVRAFAREMKDEP"
BASE COUNT          662 a         1063 c         1119 g          568 t
ORIGIN      
        1 agcgcgcatc gggtcggcga acatggtgag ccattcgtcg ggcccacgat tggaggtgaa
       61 gatcatcgca ccggcgcggt ggcgctcggt gatgatttcg tgagcgtcgc ggctctccac
      121 cgcatccatg ctgtcaagcg cgaagtcgtc gacgatcaag agatcgacgg cgaggaactt
      181 gcgcagctcc gcttcatagg agttgtcgag tcgcgcgtgc ttgagcgtct tgagcatctt
      241 gtcggcgcgc acggcaagca ccgagtgacc atgacgacag gcaatgtggc cgagcgcatg
      301 cgccaggaac gttttgccga caccaaccgg gccggcgatg atcagatgct tgtgttgctc
      361 gaggaagcgc agcgacacga gctcctgcca cagcgcctgg tcataggtga cgcgagcagt
      421 ggtgtcccac agctcgagct gcatagcagg gtcgaggcgt gcacgttgca cgcgcaacga
      481 ggtggcgaga ctgtcgcggc gggcggcttc atcggccaac accagcaaca ggaagtcctg
      541 atgagcgatc tgttgctggc gggcgagcgt gagtcgttct ggaagcgtag tgaggatcgg
      601 tgagagcttc aggcggcgca acaccgttct gaggtcgggc gagatgacat cggtcgtgct
      661 catggcagtc ctccggatca gtgctcccgc gtggtgaacg ggagagcgta ttggctcgcg
      721 ggccgcagat ggcgcgcggg cgcagtggtg gtgggcggcg gcgtagtggg cgcaacgctc
      781 gccgcctgca acatgcgatc gaggcgtcgg acgtcatgca tctgagcatc gagggcaacg
      841 ctgcacgtcg gttcgacacg cgccgcgccg tagcggcgca ccagacccaa cagcgcgtag
      901 acacggcgca tgcgtgtcca aggcagcggt ccttcgagca cctgatgagc gaagcgcccg
      961 atggcttcgc cgtgctggaa ggcttgccgc tcgaggaagg caatgtcgcg catcgcatag
     1021 gcgctcttgt gcgcggggaa gtcgttcgca tcgatcgagc ggccgcccgg gggttggcgt
     1081 ggatgcgtct tcaccagcat gccctgatcg tagaagcgca ccagggcacg atccgcgcgg
     1141 gcgcgcagat gctggccgat gaagcgggtg ggcagcgagt acagcgcctt ggcgacctgc
     1201 gcgagatgat cgcgtccgac cttcgggtcg caccagtggg gcacgtcgta gggcagcgcg
     1261 ggcgccggca gcaggtgcgg ctgctcctcg gcctcgaagc actccagcgg cagtcgctgc
     1321 gtgcgagtgt gacgccgcag cccgtactct tgccgacacc agcgctcggc gagcccacgt
     1381 gcctcagtga tcgcgtgcag cgtctcgccg gcaaagcagt cgtcgcgcac gctcggcact
     1441 gcgcgctcga cccggccctt gtcgcgggca tggcgagcgc gtgccggatc gaccacgaag
     1501 ccgcgcgcct gcgcgtactc gaggaaggtc tcgttgatgc gtgcaccgag cggatcggcc
     1561 tgcgtgacaa tcgccttggt gttatccggc agcagcactc ggaacacgcc accgaagaac
     1621 tgccacgcct gctcacacgc ctcgatcgcg ctcgccgtgg tctcgtgcaa gcacggccac
     1681 acgaagcggt ggcgcgagta gacggcggtg aagatccagg cgcgaaagcg tcggcgcttg
     1741 ccgaacacat cgggctcgag agagcccatc cagccggtat cgacctgcag ctcctcgccc
     1801 ggcgcgcagt cagcgatcgg cacggtggcg gcgctgcggc cgaagccgag ctcggcaacc
     1861 gcgaagcgat gcagcgtcgc gtagggcact tgcacgccat ggcgcagcag gagcttgcgc
     1921 agcttcgaca accgcagtcc ttgcgcgagc cactgtgcga ttcgctcgcg atgcgcttcg
     1981 cagcgctccc aggcctcccc acgcggacga cccggctgcg tcgtacgcag cgatgcgagc
     2041 atcgccgtga gcagctcatc ggtgagcgcg ctctcgccgc tggtgcgtgt cagtccggcc
     2101 acctgcgctg cacgcacgta gcggcgcacg gtcttgacgt ccaatcccag ccgcgtggcg
     2161 atgcgcttat ggcgggcgcc gcctaaccat tgccgcagaa cttctttcgt ctccacgctc
     2221 gtgacctcgc gatacgccat ggcggggttc tcctccggtc acaggccaag tttggctgga
     2281 ccgcaaggat cgcccgatgc gctgcgtcac gcgccgtggg aggggggaat gattttgaga
     2341 aactctccct cagcgggggg aaaggttctg agaaatcctc acccgagcgg ggggaatggt
     2401 tctgagaaaa tcagtgccgg cggggtccat aggttctgag aaccgacacg gataagcacc
     2461 atctgctggc cattgtcgta actcatggtt cgtccttcat ctcacgagca aacgccctga
     2521 cggcggcagc acattcttgt ggcttcgatt gcagcagaaa atgggggcct tcaatcgaaa
     2581 ccaccttcgt ccctggcacg cgcgctgaga ttaccgccga ggcagacgag aaaactaccc
     2641 tgtcttcaga agcccggagg tacagcagcg ggacccggat gtgacataga gatgacgtct
     2701 tatcgatgga cagaacagcc ttgagccccg ccctccacac ctgcggcgta actgctcgaa
     2761 tggcgcgact gagcacagcc tctttggctt cggagcgaaa gcgaccgaac aacattctgg
     2821 aagccaatga catcggagcg cgccagagag gtaacaatcc gagagccttg aaacagggca
     2881 acgcaaagga cggaactggg aagcgcgcga aggtgcagac gaggaccagt gccttgagct
     2941 gcggcggcgg atttgcggcc agtgcgatgg ccaggggccc cgaaaaagac tccgccaaca
     3001 ggatcagcga ttcatctgct ggaagaagcg agcgcacgtg gtgctcgagc tcctcgtacc
     3061 caaatggccg atccgtcgga taggagataa cgatcgatct gcagtcgagc tccgaagcga
     3121 actcatcgaa aaggacgccg gacccgtcca ttcctggcag gagaactact gctaccactc
     3181 ttctttcgct ctaacgacaa ccgtcagccg tgcgcggctt cgcacgtcgg ctccaagttt
     3241 gtcagtcctc tttggattcg catatttccc aaccgcgttt ccacacgttc cgctcaccgg
     3301 tacagtcgcc gatctcacca aacgccaaca gcaggcaaac aaagaccatc aggaacggaa
     3361 cgaacgagag ccagtctgcg gctcgacgcg tcaagtccca ttcgctcaca aa
//
LOCUS       DASURG010000043         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_725178, whole genome shotgun sequence.
ACCESSION   DASURG010000043 DASURG010000000
VERSION     DASURG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725178"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1506)
                     /locus_tag="VFY80_00705"
     CDS             complement(22..1506)
                     /locus_tag="VFY80_00705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6006447.1"
                     /translation="MGALPTLSTVIWLPILAGIVILFTGSDRNAGMARLLALVSSIAG
                     FLVAIPLWTGFDPSASGFQFVERHVWIESFNVYYHLGIDGISLLLILLNCVTTVLVVI
                     AGWKVIDTRVAQYMASFLILSGMMNGVFCALDGLLFYVFFEATLIPMFIVIGVWGGPN
                     RVYAALKFFLYTLMGSLLALIALIYLYLVSGGSFSLQDWYATPIPFVAQVLVFIALFL
                     AFAVKVPMVPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFLRLSLPIVPDASHALA
                     GLVIALSLIAVIYIGLVALVQADMKKLIAYSSISHMGFVTLGIFVFNAVAMEGAVIQM
                     VSHGFVSGALFLCVGVLYDRMHSRQIADYGGVVNRMPVFASFFMLFAMANAGLPGTSG
                     FVGEFLVIMGSMKVNFWYAAAASTTLILGAAYTLWMYKRVVFGAVANDHVAQLEDLNA
                     REWIFMAVLAALVLLMGVYPAPFVDVLHVSVNDLLAHAARSKLP"
     gene            complement(1526..>2383)
                     /locus_tag="VFY80_00710"
     CDS             complement(1526..>2383)
                     /locus_tag="VFY80_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6006448.1"
                     /translation="GFFSKDAIIEAAHLATTPGAGFAYFCVTACVFVTAFYTFRMMFM
                     TFHGPERFRQAHHASHGHVDASTAQAAASQHEAHASGHAIEASGHHDAHAHEPHESPP
                     VVTVPLIALAIPSIAAGWLIGPVLFGNYFGDAIFVAPEHQALAKMGEAFHGVVGMMAH
                     ALQTWPFWLSVAGIATAWYLYIKRPDLPAVIARRFSLVYRVLEKKYGFDEFNDWFFAG
                     GTRGLARGLWRYGDVALIDNALVNGAARLVGWCAAVIRHFQSGFIYHYAFTMIIGVLL
                     LITLLFMQS"
BASE COUNT          495 a          755 c          799 g          334 t
ORIGIN      
        1 ggctgcttct ccgttcgtgg tctacggcag cttgctgcgc gccgcgtgcg cgagcaggtc
       61 gttcacgctg acgtgcagca cgtcgacgaa cggcgccggg tagactccca tcagcagcac
      121 cagcgcggcc agcaccgcca tgaagatcca ctcgcgagcg ttcagatcct ccagctgcgc
      181 cacatggtcg ttcgccacgg cgccgaagac gacgcgcttg tacatccaca gcgtataggc
      241 ggcgcccagg atgagcgtgg tcgaggcagc cgctgcgtac cagaaattga ccttcatcga
      301 gcccatgatg accaggaact cgccgacgaa gccgctcgtg ccgggaagcc cggcgttcgc
      361 catcgcgaac agcatgaaga agctggcgaa gaccggcatg cggttgacca cgccgccata
      421 gtcggcgatc tggcgcgagt gcatgcggtc gtagagcacg ccgacgcaga ggaacagtgc
      481 gccggagacg aagccgtgcg agaccatctg gatcaccgca ccttccatcg ccaccgcgtt
      541 gaagacgaag atgccgagcg tgacgaagcc catgtgcgag atcgaggagt acgcgatcag
      601 cttcttcatg tcggcctgca ccagcgcaac caggccgatg tagatgaccg cgatgagcga
      661 cagcgcgatc acgagcccgg cgagcgcgtg gctcgcgtcg ggcacgatgg gaagcgacag
      721 ccgcaggaag ccgtacgcgc ccagcttcag catgatcgcc gccagcacca ccgacccccc
      781 ggtgggcgcc tcgacgtgcg catcggggag ccacgtgtgc acgggcacca tcggaacctt
      841 gacggcgaag gcgaggaaca gggcgatgaa gaccagcacc tgcgccacga acgggatcgg
      901 cgtcgcatac cagtcctgca gggagaagct tccgcccgag accaggtaga ggtagatgag
      961 cgcgatcagg gcgagcagcg accccatgag cgtatagagg aagaacttga gcgccgcgta
     1021 gacgcggttg gggccgcccc agacgccgat tacgatgaac atcgggatca gcgtcgcctc
     1081 gaagaagacg tagaagagca gcccgtcgag cgcgcagaac acgccgttca tcatccccga
     1141 gaggatgagg aacgaggcca tgtactgcgc cacccgcgtg tcgatcacct tccagccggc
     1201 gatcacgacg agcaccgtgg tgacgcaatt gagcaggatg agcagcagcg agatgccgtc
     1261 gatgccgagg tgatagtaga cgttgaagct ctcgatccag acatggcgtt cgacgaactg
     1321 gaacccgctc gccgacggat cgaatccggt ccagagcgga atggccacca ggaagccggc
     1381 gatcgagctc accagcgcga gcagtctcgc catcccggcg ttgcggtcgg aaccggtgaa
     1441 caggatgacg atcccggcca ggatcggcag ccagatgacg gtgctcagcg tcggcagtgc
     1501 gcccatgcgt gcctgctctc cctgctcagc tctgcatgaa cagcagcgtg atgagcagca
     1561 gcacgccgat gatcatggtg aacgcgtagt gatagatgaa accggactgg aagtgacgga
     1621 tgaccgccgc gcaccagccg acgaggcgcg cggctccgtt gaccagcgca ttgtcgatga
     1681 gcgcgacgtc gccgtagcgc cacaggcctc gcgccaggcc acgcgtgccg ccggcgaaga
     1741 accagtcgtt gaactcgtcg aagccgtact tcttctccag cacccgatac acgagcgaga
     1801 agcgccgcgc gatcaccgct ggcaggtcgg gccgcttgat gtagaggtac caggcagtcg
     1861 cgatgccggc gaccgagagc cagaaaggcc aggtctggag cgcatgcgcc atcatgccga
     1921 cgacaccgtg aaacgcttcg cccatcttcg ccagcgcctg gtgctcgggc gcgacgaaga
     1981 tcgcgtcgcc gaagtagttg ccgaacagca ccggaccgat cagccagccg gccgcgatgc
     2041 tgggaatcgc gagcgcgatg agcggcacgg tgacgaccgg cggcgactcg tgcggctcgt
     2101 gcgcgtgcgc atcatgatgg ccggatgcct cgatcgcatg gccgctcgcg tgcgcctcgt
     2161 gttgcgacgc cgcagcctgt gccgtgctcg catcgacgtg gccgtgggat gcgtggtgcg
     2221 cctggcggaa gcgctccggg ccatggaagg tcatgaacat catgcggaag gtgtagaagg
     2281 cggtcacgaa cacgcacgcg gtgacgcaga aataggcgaa accggcgccc ggcgtggtgg
     2341 cgagatgcgc cgcctcgatg atcgcgtcct tggagaagaa ccc
//
LOCUS       DASURG010000044         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1236008, whole genome shotgun sequence.
ACCESSION   DASURG010000044 DASURG010000000
VERSION     DASURG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236008"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFY80_00715"
     CDS             <1..1077
                     /locus_tag="VFY80_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6006449.1"
                     /translation="TVSATGKLQPTNQVDVGSELSGTIEQVFVDDNDLVKQGQVIARL
                     DVSKLKDQVAKSRAAVAAAEAQLKQMQATEAEARANLARLRQVAVLSGGKVPSKAEME
                     TAEASVARAIANIASARATIAQAVASLRSDETNLAKASIRSPINGVILTRKVEPGQTV
                     AASFQAPVLFTIAEDLTKMRLEVDVDEADVGSVREGQSATFSVDAYPNRRYPSDVTRV
                     GYGAQTKENVVSYLTVLTVDNSDLSLRPGMTGTADIVTAQRSNVLLVSNAALRFSPPP
                     TNAAAKKSGGLLSAMLPRPPRSQTRPTARAPGEAQRVWVLRDGTPTPIAVTAGPTDGR
                     MTELTGGEVQEGMQVITEAYSAPK"
     gene            1080..1769
                     /locus_tag="VFY80_00720"
     CDS             1080..1769
                     /locus_tag="VFY80_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6006450.1"
                     /translation="MIALTGITKTFGAGKAAFQALRGVDLALDEGEFVAVMGPSGSGK
                     STFMNILGCLDMPSTGEYLFREVHVERLSRRQRALLRRYFIGFVFQGFNLLGRTTALE
                     NVELPLLYRGSARSARHATARDALASVGLGGWEHHTPAELSGGQQQRVAIARALVTKP
                     AVLLADEPTGNLDTRTSREIMDLLAALNRDHGITIVMVTHEPDMAQYAKRIVHFVDGR
                     VESDTEQPAVH"
     gene            1769..>2449
                     /locus_tag="VFY80_00725"
     CDS             1769..>2449
                     /locus_tag="VFY80_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6006451.1"
                     /translation="MLWNTFLLALRAILRNLMRSVLTMLGIVIGVSAVITMVTLGNGA
                     TQSVRAQIAGMGSNLLIVLPGQGFGPGAGDAPRFRIADAESLRAQLSTAQAVAPTVTR
                     RTTAVYGARNWMTAVIGTTSAYFTAGNWTLAEGRTFTDPEVRAGASVCVLGDSVRRNL
                     YGNQNPVGATLRIGQFSCSVIGLLRPKGQSAMGSDQDDIIAMPLRTVQRRLTGTQDVA
                     SIMVSITDS"
BASE COUNT          444 a          807 c          820 g          378 t
ORIGIN      
        1 accgtgtcgg caaccgggaa actgcagccg acgaaccagg tcgacgtcgg gagcgaactt
       61 tcgggcacga tcgagcaggt attcgtggac gacaacgatt tggtcaagca gggccaggtg
      121 atcgcgcggc tcgacgtttc gaaactgaag gaccaggtcg ccaagtcgcg cgcggcggtg
      181 gcggccgcgg aggcgcagtt aaagcagatg caggcgacgg aggcggaagc acgggcaaat
      241 ctcgcgcgct tgcggcaagt cgccgtgctc tcgggcggaa aggtaccgtc caaagccgag
      301 atggaaacgg cggaagcgag cgtcgcacgc gcaatcgcga atatcgcgag cgcccgcgca
      361 acaattgcgc aggccgtcgc ctcactccgg tccgacgaga cgaacctcgc gaaggcttcg
      421 attcgctcgc cgatcaacgg cgtgatcctc acgcgcaagg tggagcccgg gcagacggtt
      481 gcggcctcgt ttcaggcgcc ggtgctcttc accatcgccg aagacctcac gaagatgagg
      541 ctcgaagtcg acgtcgatga agccgacgtc ggcagcgtgc gggaaggaca gagcgcaacg
      601 ttcagcgtgg acgcctatcc caatcggcgc tacccgtccg atgtgacgcg cgtgggctac
      661 ggcgcgcaga ccaaggagaa cgtggtctcc tatctgacgg tgctcaccgt ggacaactcc
      721 gacctgagcc tgcgccccgg catgacgggc acggcggaca tcgtgaccgc ccagcgcagc
      781 aacgtgctgc tcgtgtccaa tgccgccctg cggttctccc ctccgccaac gaatgcggcg
      841 gcgaagaagt cgggcgggct tctcagtgcc atgctgccgc gtcccccgcg ctcgcagacg
      901 cgccccaccg cgcgggcacc gggcgaagcg cagcgcgtct gggtgctgcg cgacggtacc
      961 ccgacgccga tcgccgtcac tgccggtccc accgacggcc gcatgaccga acttacgggc
     1021 ggcgaagtgc aagagggtat gcaagtaatc acggaagcgt acagcgcacc gaagtgaagc
     1081 tgatcgcgct caccggtatc accaagacct tcggcgccgg caaggcggct tttcaggcgc
     1141 tgcgcggggt agacctcgcg ctcgacgaag gggagttcgt cgcggtgatg gggccgagcg
     1201 gctcgggtaa atccactttc atgaatatcc tgggctgcct cgacatgccg agcacgggcg
     1261 aatatctctt ccgggaggtg cacgttgagc gcctctcccg tcggcagcgg gcgctgctgc
     1321 ggcgctattt catcggattc gtgttccagg gcttcaatct cctgggacgc acgacggcac
     1381 tggagaatgt cgagctgccg ctgctctacc gcggttccgc gcgaagcgcg cgtcacgcca
     1441 ccgcacgcga tgcgctcgcc tcggtcgggc tcggtggctg ggaacaccat acgcctgcgg
     1501 aactctcggg cgggcagcag cagcgcgtcg ccatcgcgcg cgcgctcgtc acgaaaccgg
     1561 cggtgctgct cgcagacgag cccaccggca atctcgacac gcggacgagc cgcgagatca
     1621 tggacctcct cgcggcactc aatcgcgacc acggcattac catcgtgatg gtgacgcacg
     1681 agcccgacat ggcgcagtat gcgaagcgca tcgtgcactt cgtcgacggg cgtgtggagt
     1741 ccgataccga gcaaccggcg gtgcactgat gctgtggaac accttcctcc tcgcgctgcg
     1801 cgcgatcctg cgcaacctga tgcgctcggt cctcaccatg ctcggcatcg tgatcggcgt
     1861 ctcagccgtg atcaccatgg tgacgctcgg aaacggcgcc acgcagtcag tgcgcgcgca
     1921 gatcgccggc atgggaagca acctgctgat cgtgctcccc ggccagggat tcggtccggg
     1981 tgcgggcgat gcaccgcgtt tcaggatcgc cgatgcagaa tcgctccgcg cgcagctcag
     2041 tacggcgcag gcggtggccc cgaccgtgac gcggcgcacg accgcggtct acggagcacg
     2101 caactggatg acggccgtca tcggcacgac gagtgcctac ttcaccgcgg gaaactggac
     2161 gcttgccgag gggcgcactt ttaccgatcc agaggtgcgg gccggtgcat cggtctgcgt
     2221 gctcggggat tcggtccgcc gcaacctgta cggaaaccaa aacccggtcg gcgccacgtt
     2281 gcggatcggg cagttctcct gctcggtcat cgggctgctg cggcccaagg gtcagtccgc
     2341 gatgggctcc gaccaggatg acatcatcgc catgccgctg cggacggtgc agcgacggct
     2401 caccgggaca caggacgtcg catcgatcat ggtctcgatc accgacagc
//
LOCUS       DASURG010000045         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1441167, whole genome shotgun sequence.
ACCESSION   DASURG010000045 DASURG010000000
VERSION     DASURG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441167"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /gene="uppS"
                     /locus_tag="VFY80_00730"
     CDS             complement(<1..586)
                     /gene="uppS"
                     /locus_tag="VFY80_00730"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX6006452.1"
                     /translation="MFESSTEQIPASRDMPSHIAIIMDGNGRWAKRRFLPRMAGHKRG
                     VEAVRRMVRACAERGIAYLTLFAFSSENWRRPPEEVSFLMRLFMLSLDQEVGKLHENG
                     IRFRVIGDLSRFEPELVRLIRDAETLTAGNDRLILTVAANYGGRWDIMQAVKRMLAEH
                     PELATSFTESDLRSYFALGHTPEPDLFIRTGGEQR"
     gene            complement(622..1182)
                     /gene="frr"
                     /locus_tag="VFY80_00735"
     CDS             complement(622..1182)
                     /gene="frr"
                     /locus_tag="VFY80_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX6006453.1"
                     /translation="MMIADVKKTAEQKMKRTLEALKADLGKVRTGRVHTGLLDHIMVD
                     YYGTPTPVPQVGNVTLLDARTIGVTPWDKKMIAPVEKAIRDSDLGLNPGAQGDTIRVP
                     MPPLTEERRRELIKVVRHEGENARVAVRNVRRDAIHHLKELLKQKKVAEDEERRAQED
                     IQKLTDRHIGEIDKMLQAKEADLMAI"
     gene            complement(1194..1913)
                     /gene="pyrH"
                     /locus_tag="VFY80_00740"
     CDS             complement(1194..1913)
                     /gene="pyrH"
                     /locus_tag="VFY80_00740"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6006454.1"
                     /translation="MPETPAYKRILLKLSGEALMGEDSYGINRDTIERIVDEIAEVVN
                     LGVEVAVVIGGGNIFRGLAPGAAHMDRATADYMGMLATVMNALALQDAMRRVNLVSRV
                     QSALNIEQVVEPYIRGKAMRYLEEGKIVIFAAGTGNPFFTTDTAAALRGMEMNVNLVL
                     KATKVDGVYTDDPKANPEAMRYKTLTFDEAIIKNLKVMDATALTLCRDQKLPVNVFSI
                     FKPRALRRVVVGEDEGTMVRA"
     gene            complement(1960..>2695)
                     /gene="tsf"
                     /locus_tag="VFY80_00745"
     CDS             complement(1960..>2695)
                     /gene="tsf"
                     /locus_tag="VFY80_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6006455.1"
                     /translation="KAASRITAEGIVAAYVNDERAVGALIEVNCETDFVAKNTEFAGF
                     AGTVAQLVAQQAPADVAALSALKLGAGTVEETRKALIGKIGENITLRRFSRIAARGRL
                     HTYIHGGAKIGVLVDVVSGDDALGKDLAMHIAASKPIALDAVDVAPEVVRKERDIAAA
                     KAAESGKPGNIVEKMVEGSVQKFLKEVTLLGQPFVRNDKQSVGQLLKSKGASVASFTL
                     YVVGEGLERRKEDFAAEVMAQVASQR"
BASE COUNT          436 a          885 c          793 g          581 t
ORIGIN      
        1 tccgctgctc cccgccggtg cggatgaaaa gatccggctc cggcgtgtga ccgagcgcaa
       61 agtaggacct gagatcgctt tccgtgaacg atgtcgcaag ttccggatgc tcggccagca
      121 tgcgcttcac tgcctgcatg atgtcccaac ggcccccgta gttggccgca acagtaagta
      181 tgagacgatc gttccccgcg gtgagtgtct ccgcgtcacg gatgagacgc accaattccg
      241 gttcgaagcg ggagagatcg ccgatcaccc tgaagcgaat accgttctcg tgcagcttcc
      301 cgacttcctg gtcgagcgag agcatgaaca gccgcatcag gaaagacact tcctcgggag
      361 gacgccgcca gttttcgctg ctgaaggcga agagcgtgag ataagcgatg ccgcgctcgg
      421 cacacgcgcg aaccatgcga cgcaccgcct ctaccccgcg cttgtgcccg gccatgcgcg
      481 gcaaaaagcg gcgcttcgcc cagcgcccgt tgccgtccat gatgatcgcg atgtggctcg
      541 gcatgtcacg cgatgctgga atctgctccg tcgagctttc gaacattcgt ctccctgctt
      601 tcgtgcgatc gccggcaatc gtcagattgc catgagatcg gcttccttcg cctgcagcat
      661 cttgtcgatc tccccaatat gccggtcggt gagcttctgt atatcctcct gcgcccgccg
      721 ttcctcgtcc tcggcaacct tcttctgctt gagcagctct ttcagatgat ggatcgcgtc
      781 gcggcggacg ttgcgcaccg ccacccgcgc attttcgccc tcatggcgca cgactttgat
      841 cagctccctg cggcgctctt cggtcagggg cggcatcggc acgcggatgg tatcgccctg
      901 cgcgccgggg ttgaggccga gatcggagtc gcggatcgcc ttttcgaccg gcgcgatcat
      961 cttcttgtcc catggcgtta cgccgatcgt gcgcgcatcg agcagcgtca cgttccccac
     1021 ctgcgggacg ggcgtgggcg tgccgtagta gtccaccatg atgtgatcga gaagccccgt
     1081 atggacgcgc ccggtgcgca ccttcccgag gtccgccttg agcgcttcga gcgtcctctt
     1141 catcttctgt tcggcggtct ttttgacgtc ggcaatcatg atccacctcc gttctatgcg
     1201 cgaaccattg taccctcgtc ttcgcccacc accacgcgcc gcagcgctct cggcttgaag
     1261 atgctgaaga cgttgactgg cagcttctgg tcacggcaga gcgtgagcgc cgtcgcgtcc
     1321 atgactttca ggtttttgat gatggcctcg tcgaaggtga gcgtcttgta gcgcatcgcc
     1381 tccgggttcg ctttcgggtc gtcggtgtag acgccgtcca ctttggtcgc cttgagcacg
     1441 agattcacgt tcatctccat gccgcggagt gcggcggcgg tatcagtggt gaagaagggg
     1501 ttgccggtgc ctgccgcaaa aatgacgatc ttgccctctt cgaggtagcg catggccttg
     1561 cctcggatat acggctccac cacctgctcg atattgagcg ccgactgcac ccggctcacg
     1621 agatttaccc ggcgcatggc gtcctggagc gcaagcgcat tcatgacggt tgcaagcatg
     1681 cccatgtagt cagcggtggc gcggtccata tgagcggcgc cgggcgcaag cccgcgaaag
     1741 atgtttccgc cgccgatgac gaccgcgact tccacgccga gattgaccac ttcggcgatc
     1801 tcatcgacga tgcgctctat ggtgtcccga ttgatgccat agctgtcctc ccccatgagt
     1861 gcctcgccgc tcagcttgag aaggatgcgc ttgtaggcgg gcgtctcggg catgggtttt
     1921 ctcgttgttc tcggtgcgca ctctagcgca gggccgcgct catctttgcg aggccacctg
     1981 cgccatcact tccgctgcaa aatcctcctt gcgccgctcg aggccttcac cgacgacgta
     2041 gagcgtgaag ctcgcgaccg aggcgccctt cgatttcagc agctggccca ccgactgctt
     2101 gtcgttcctg acaaacggtt gcccgagcag cgtcacctcc ttcagaaact tctgcaccga
     2161 gccttccacc atcttctcga cgatgttgcc gggctttccg gattccgcag ccttcgccgc
     2221 tgcaatgtca cgttctttcc ggacgacttc cggcgccacg tcgacggcat caagggcaat
     2281 cggcttgctt gccgcgatat gcatcgcgag atccttgccg agcgcatcgt ccccgctcac
     2341 gacatccacc agcacgccga tctttgcgcc gccgtggatg taggtatgga gccggccgcg
     2401 cgcggcgatg cgcgagaagc gccgcagcgt gatgttctct ccgatcttgc cgatcagcgc
     2461 cttgcgggtt tcttccacgg tgccggcgcc gagtttcagc gcggacaacg ccgccacgtc
     2521 cgcgggggcc tgttgtgcga ccaactgggc gactgtgccg gcgaaacccg caaattcggt
     2581 attctttgcg acgaaatccg tttcgcagtt cacttcgatg agcgctccga cagcgcgctc
     2641 gtcgttcacg taagcggcga cgatgccctc cgcagtgatg cgggaagcgg ccttg
//
LOCUS       DASURG010000046         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_419482, whole genome shotgun sequence.
ACCESSION   DASURG010000046 DASURG010000000
VERSION     DASURG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419482"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>2952
                     /locus_tag="VFY80_00750"
     CDS             53..>2952
                     /locus_tag="VFY80_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX6006456.1"
                     /translation="MAFILIQHLDPTHQSMMAELLSGHTSMKVQNAADGMRIEHDCVY
                     VIPPGVYLSIRDGALHLSHPQERHGARAPFDFFLRSLAEEQAERAVCVILSGTGTDGS
                     LGLKAIKENGGFAIAQDPHEAVHDGMPRNAIMTGAVDLVLPVAKIPAALAEYSRQLRA
                     PHKRSDAGAVDESAHAISGIIALLRTQAPLDFTLYKPGTLQRRIERRAAMAADGDLSR
                     YLAVLNRDARELDLLAKDLLINVTSFFRDPPAFELLANKIVPDLVRRHAPDQPLRIWI
                     AGCSTGEETYSIAMLLREGLDAAGRNIRLQIFASDVDPDAVAFAREGLYPRSIEADVS
                     PTRLDRFFAKEDRGYRIIPALRELVVFAVQDVLADPPFSRLDLISCRNLLIYLRPEAQ
                     QKILLLFHFALREGGILFLGGSETAGGSDRYFEPISKTQRIYRHVGRIRPGERDFPAS
                     ARTGVRTPVRPEAPWTIPADTQLREAAQQLLIETYAPASVVINYKCETLYYLGPVDRY
                     LRVASGKPSRDLLAMAREGLRNKLRTAVHQAIRERAYAVNSGARLKRNGASITVTIAV
                     HPIESANLELLLVSFLEEAERTEEPAVTTTQGGSASRVADLERELDATRSDLQSTISE
                     LERANEEQKAVSEEAMSVSEEFQSTNEELVTSKEELQSLNEELTALNSQLQETLEQQR
                     NTSNDLQNILHSSAIATLFLDSSLNIRFFTPAATSLFSVIATDIGRPLADLTPLSSDA
                     NLLSDARTVLATLMPIRREIQGQGAAWYIRSVLPYRTHDDRVEGVVVTFTDLSETKAA
                     EARIEAARAYSESIINTIRQPLLVLDSELRIISGNNAFYRFFALTADRAVGRPFAAVS
                     DQLAAMAELPAFLDRVRTAPGSISDCEIEAELPELGRRALLFNGRKIHDGLPGQAKIL
                     LAIDDITDRKRAAEALEAAKRRAEQANLGKSRFLAAASHDLRQPLYSLSLLTA"
BASE COUNT          594 a          986 c          833 g          539 t
ORIGIN      
        1 cggcctcgat gcgttccaga agttcttcga cgccgtgcca cagggcagcg gcatggcctt
       61 catactgatc cagcatctcg atccgacgca ccagagcatg atggccgaat tgctgtctgg
      121 acacacatca atgaaggtgc agaacgccgc cgatggaatg cggatcgagc acgactgcgt
      181 ctatgtcatt ccacccggtg tctatctttc gattcgggac ggcgcgcttc atctttccca
      241 tcctcaggag cgtcacggtg cccgtgcgcc cttcgacttc tttctacgct cgctggcgga
      301 agagcaagcc gagcgcgccg tttgcgttat cctttcgggg acgggaaccg acggcagcct
      361 cgggctgaag gcgatcaaag agaacggcgg gttcgccatc gcacaggacc cccacgaagc
      421 ggtccacgac ggcatgccgc ggaacgcaat catgacaggc gcggtcgatc ttgtgcttcc
      481 agtggcgaag attccggcgg cgctcgccga atattcgcgg cagctccgcg cgcctcacaa
      541 gcgaagcgac gcaggtgccg ttgacgaatc cgcgcatgcg atctccggca tcatagctct
      601 tctgcgcacg caggcgccgc tcgacttcac cctgtacaag ccgggcacgc tgcagcgccg
      661 aatagaacgc cgcgccgcga tggccgcgga tggcgaccta agccgctacc tggcggtgct
      721 gaaccgcgac gcccgcgaac tggacctcct cgccaaggac ttgctgatca acgtcaccag
      781 cttcttccga gatcctccgg cattcgagct tctggcaaac aagatcgttc ccgacctcgt
      841 gcgccggcac gcaccggatc agccgctcag aatctggatc gccggatgta gcaccggcga
      901 agagacttac tcgatcgcca tgctcctccg cgagggactc gatgcagccg ggcgcaatat
      961 aaggcttcag atctttgcct ccgatgtcga tcccgacgcc gtcgccttcg cgcgtgaagg
     1021 cctctacccg agatcgatcg aagcggacgt atcgccgacg cgcctggatc gcttcttcgc
     1081 aaaggaggac cgcggctacc gcataatccc ggcgctgcgc gagctcgtgg tcttcgccgt
     1141 tcaggacgta ctggccgacc ctcccttctc tcgtctcgat ctgatctcct gccgcaacct
     1201 tctcatctac cttcgtcccg aggcgcagca aaaaatcctc cttctctttc atttcgcctt
     1261 gcgagaaggc ggcatcctct ttctcggcgg gtccgagacg gcgggaggaa gcgatcgtta
     1321 tttcgaacca atctccaaga cccagcggat ctaccgccat gtcggtcgca tccgacccgg
     1381 cgagcgcgat tttcccgcct cggccagaac cggcgtgcga acccccgtgc ggccggaagc
     1441 gccgtggacg atacctgccg atacgcaact ccgtgaggct gcgcaacagc ttttgataga
     1501 gacctatgcc cccgcctcgg ttgtgatcaa ctacaaatgc gagaccctct attacctggg
     1561 tcctgtagat cgctacctgc gtgtggcctc gggcaaaccc agccgcgact tgcttgcgat
     1621 ggcgcgggag ggcttgcgca acaagctcag gaccgcggtc catcaggcta tccgagagcg
     1681 tgcttacgcg gtcaatagcg gggcaaggct taagcgcaat ggcgcgtcga tcacagtcac
     1741 gatcgcagtc cacccgatcg aaagcgcgaa ccttgagttg ctgctggtga gttttctgga
     1801 agaagccgag cggacggaag agcccgccgt aacaaccacg cagggcggca gcgcgtcgcg
     1861 cgtcgcggac ctcgagcgtg agctcgatgc cacgcgcagc gatctacaaa gcacgatcag
     1921 cgaactggag cgagcaaacg aggaacagaa ggcggtcagc gaagaagcca tgtccgtcag
     1981 cgaggagttt caatcgacga acgaggagtt ggtaacgtcg aaggaagagc tgcagtcact
     2041 caacgaagag ctgaccgccc tcaacagcca actccaagaa accctcgagc agcagcgcaa
     2101 tacatccaac gacctgcaga acatcctgca cagctcggca attgcgacac tcttcctgga
     2161 cagcagcctc aatattcggt tcttcacgcc ggccgcgaca tcgctcttca gcgttatcgc
     2221 caccgacatc ggccgcccgc tcgccgacct cacccctctt tcctccgacg caaatcttct
     2281 ctccgatgcc cgtaccgtac tcgcgaccct catgccgata cggcgggaaa ttcaagggca
     2341 aggcgctgcc tggtatatcc gcagcgtctt gccttatcga actcatgacg atcgggtcga
     2401 aggcgtcgtg gtcactttca ccgacctgtc cgaaacaaag gctgcggaag cacgcataga
     2461 ggcagcccgc gcgtattccg agagtatcat caataccatc cgccagccgc tcctggtgct
     2521 cgactcggag ctgcgcatca tatcgggcaa caatgcgttt tatcgcttct tcgccctcac
     2581 agccgaccgc gctgtcggcc ggcccttcgc ggccgtcagc gatcagctcg cggctatggc
     2641 ggagctccct gcctttctcg atcgggtccg aaccgccccc ggttccattt ccgactgcga
     2701 gatcgaggcc gagttgccgg agctcggccg aagagccctg ctcttcaacg ggcgaaagat
     2761 ccatgacggg ttgcccggac aagcgaagat cttacttgcc atcgacgaca ttaccgaccg
     2821 aaagcgggct gcggaggcgt tggaagcagc caaacgacgt gccgagcagg caaacctcgg
     2881 taaatcgcga ttccttgccg ccgcgagcca tgacctgcgc cagccgctgt actccctgag
     2941 cctgctcacc gc
//
LOCUS       DASURG010000047         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_828036, whole genome shotgun sequence.
ACCESSION   DASURG010000047 DASURG010000000
VERSION     DASURG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828036"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1139
                     /locus_tag="VFY80_00755"
     CDS             171..1139
                     /locus_tag="VFY80_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006457.1"
                     /translation="MKSLAFIVCALALLGSGTMPASAQSYPAKPIRLVVGFTPGGGVD
                     INARLLGPKITEYLGQQVIVDNRPGAGTNIANEHVAKSPPDGYTLLINTAAVAINMSL
                     YKKVPFDTLRDFAPVSVFSQSPNILVVHASVPAKNVKELLALARAKPGALNYSSAGSG
                     TTQHLTGELFKLRTKTDIVHVPYRGSAPSLTALIGGEVSLSFANIPAIHNHVKSGRLR
                     PLANAGPKRSPLMPEVPTMGEAGVKGVEVVVWYGVLAPANTPKGVIDTLAGAIRKAAQ
                     SPDIRQRLADQGAEPVGNTPEEFAKLLREEVTRWAEVVKVSGAKVD"
     gene            complement(1146..2312)
                     /locus_tag="VFY80_00760"
     CDS             complement(1146..2312)
                     /locus_tag="VFY80_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006458.1"
                     /translation="MTIWLVIALTLLTHTAFKGSRVVISLYAIQFGATPFEIGILFSM
                     YSVFPVLLSVYAGKISDRLGFRLPMMFGACGLMIGLSIPFFFPRLAALYASAVLIGMC
                     YIFYTVTVQHFIGAAGEGHERTRNYSIFSLGIAVTSMLGPTSAGFAIDLIGHRSTYLM
                     LAAMPALPFLALVLFPRLLPHLHVKEEARHGQRVMDLVRDPPLRRALIAAGLVETGLE
                     LFNFFLPIYGHYSGLSATQIGICMGAFAAALLLVRGLMPTLTRRSSEEAVLSGSLFLA
                     AGTALLFPLVSSFVLLSATSFVLGLGLGCGSPLSMILAYNRAPPGRSGEAMGLRQTVN
                     KATEVAMPIVFGSLSTALGMPPVFWMEALILAWAGALARRDAAARKAASPSVSR"
     gene            complement(2319..3233)
                     /locus_tag="VFY80_00765"
     CDS             complement(2319..3233)
                     /locus_tag="VFY80_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6006459.1"
                     /translation="MSRQAQTMAVLFADICDSTGLYQKLGDATARNVVNACLSTISSV
                     LPRFDGRVVKTIGDEILCVFPSTDLAVLAASEMQSAVSAATPSNYPVKIHIGLHYGPV
                     LTENEDVFGEAVNVAAYLTAVATPEQILTTESTEKCLSAALKSCVRPVFRVLLKGNTK
                     ESTVYQVLWRIEDEHKTDVNVHSDKLIPGDTGSLVLTLGEQTVRLDQWVPSVTVGRAA
                     DCSLVVTSKFASRRHLTVKLMRTNVYLLDHSINGTFVCLEGGHEIHVLRSELLLEGKG
                     HIILGRSRVENPTEIIQFARDRRSMYRL"
     gene            complement(3314..>3667)
                     /locus_tag="VFY80_00770"
     CDS             complement(3314..>3667)
                     /locus_tag="VFY80_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX6006460.1"
                     /translation="RQVYSVDIIQELAEGAGKRLKRLGYTNIEVRVANGYYGLPEHAP
                     YDNIIVTAACELIPPPLLAQLKPGGRMVIPTGIPEKQALTLVEKSASGKLATRDVLPV
                     RFSELEEPGAVAGLA"
BASE COUNT          711 a         1143 c         1192 g          621 t
ORIGIN      
        1 tgccggtcgc tcgttgcatc ccatgaggag cacgagcatc aacgacaccg tcatcgagac
       61 gcgcgccatg cgttggcatt tcgaacgagg aaccgcgcta tgttaacagt ttgccatttg
      121 ctaccattac ggctcttccg ctccgtttcc tgaacaggag atctcccgcg atgaaatcgc
      181 tcgcgttcat agtgtgcgca ctcgctctgc ttggcagcgg cacgatgcct gcctccgcgc
      241 agagttatcc cgcgaaaccg atccggctcg tcgtcggctt cacgccgggc ggcggggtcg
      301 atatcaacgc gcgcctcctg gggccgaaga tcaccgagta cctcggccag caggtgatcg
      361 tagacaatcg tcccggggca ggaacgaata tcgcgaacga gcacgtcgca aagtcgccac
      421 ccgacggtta cacgctgctc atcaacaccg ccgcggtcgc gatcaacatg agcctttaca
      481 agaaagtgcc attcgacacg ctgcgcgact tcgcgcccgt ttcggtgttc tcgcaaagcc
      541 ccaatatcct cgtcgttcac gcatcggttc cggcgaagaa cgtgaaggag ttgcttgcct
      601 tggcacgcgc aaaaccgggc gcgctcaatt attcatcagc gggcagcggc accacgcagc
      661 acctgaccgg tgagctcttc aagctgcgca cgaaaaccga catcgtgcac gtgccctaca
      721 ggggcagcgc gccgtcgctc accgctttga tcggaggcga agtctcgctt tccttcgcca
      781 acatcccggc gatacacaac cacgtcaaat ccgggcggct gcgcccgctc gcgaacgccg
      841 gcccgaagcg ctcgccgctc atgcctgagg tgccgacgat gggggaagcg ggcgtcaaag
      901 gcgttgaagt ggtggtgtgg tacggtgtgc tggcgcctgc caatacgccg aagggcgtca
      961 tcgatacgct ggcaggcgcg atccgcaagg cggcgcaatc gccggacatc cggcagcggc
     1021 tcgcggatca gggcgccgag ccggtcggca atactccgga ggaattcgcg aagctgctgc
     1081 gagaggaagt cacgcgctgg gcggaagtgg tcaaggtctc cggcgccaaa gtcgattaac
     1141 ggcgctcacc tggacacgct cggcgaagct gccttgcggg cagccgcatc ccgccgtgcg
     1201 agcgcgccgg cccaggcgag gatgagcgcc tccatccaga ataccggcgg catgccgagt
     1261 gcggtgctga gcgacccgaa cacgatcggc atcgcgactt cagtcgcctt gttcaccgtc
     1321 tgacgcagcc ccatcgcctc gccactgcgt ccgggcggtg cgcgattgta ggcgagaatc
     1381 atggaaagcg ggctgccgca gcccaacccg agcccgagca cgaaggaagt cgcgctcagc
     1441 agcacgaagc tcgagacgag cggaaagagg agtgccgtgc ctgccgcaag aaagagcgag
     1501 ccgctcagca ccgcttcctc gctggaacgg cgcgtgaggg tcggcatgag cccgcgcacg
     1561 aggagcagcg cggcagcaaa ggcgcccatg cagatgccga tctgcgtggc cgaaagcccg
     1621 ctgtagtggc cgtagatcgg gaggaagaaa ttgaagagct cgagcccggt ctcgacgagc
     1681 cctgcggcga tgagcgcgcg gcgcagcggc ggatcgcgca cgaggtccat gacgcgttgc
     1741 ccgtggcgcg cctcttcctt gacgtgcaag tgcggcagca gtcgcggaaa caacacgagc
     1801 gcgaggaacg gcaatgccgg catcgccgcg agcatgagat aggttgagcg gtgaccgatg
     1861 agatcgatcg cgaatccggc gctggttggg ccaagcatcg acgtgaccgc aatccccagg
     1921 ctgaagatgc tgtagttgcg cgtgcgctcg tgcccttctc ccgccgcacc gatgaagtgc
     1981 tgaaccgtga ccgtgtagaa gatgtagcac atgccgatca gcacggcgga tgcgtagagc
     2041 gccgcgaggc gcggaaagaa gaagggaatc gaaagcccga tcatgagccc gcaggcgccg
     2101 aacatcatcg gtagccggaa tccgaggcgg tccgagatct tgccggcgta aacggagagc
     2161 agcaccggaa acaccgagta catcgaaaac agtattccga tctcgaacgg cgtcgcgccg
     2221 aactggatcg cgtagagcga tatgacgacc cggctgccct tgaaagcggt gtgcgtgagg
     2281 agtgtgaggg cgatgacgag ccagatcgtc atcgcgactc agaggcggta catcgaccgg
     2341 cggtcccgcg cgaactgaat gatctcggta gggttttcga cgcggctgcg gccgaggatg
     2401 atgtgccctt tgccctccag cagcagctcg ctccgcagca cgtgtatttc atggccgcct
     2461 tccaggcaga caaaggtgcc gttgatgctg tggtccagga gatagacgtt cgtgcgcatc
     2521 agcttcacgg tgagatgccg gcgcgaggcg aacttactgg tgacgacgag actgcaatcc
     2581 gccgcacggc cgaccgtcac cgacggcacc cactggtcga ggcgcacggt ttgctcgccc
     2641 agcgtgagga caaggctgcc ggtatcgccc gggatcagct tgtccgagtg cacgttgacg
     2701 tccgtcttgt gctcgtcctc gatgcgccac agcacctgat agacggtcga ttctttcgtg
     2761 ttgcccttga gcagcacacg gaacacgggt cgcacgcacg atttgagcgc ggcggacagg
     2821 catttctccg tgctttcggt ggtcaggatc tgttccggcg tcgcgacggc ggtgagatac
     2881 gctgcgacgt tcaccgcctc gccgaagacg tcttcgttct cggtcagcac cggcccgtag
     2941 tgcaggccga tgtggatctt caccgggtaa ttgctgggcg tcgcggcact tacagcggat
     3001 tgcatctcgc ttgccgccag cacggcgaga tcggtgctgg gaaagacgca caggatttcg
     3061 tcgccgatgg tcttcaccac ccggccgtcg aagcgcggca gcacgctcga aatggtggag
     3121 aggcaggcgt tcaccacatt gcgagcggtt gcgtccccga gcttttgata gaggccggtg
     3181 ctatcgcaga tgtccgcgaa cagcacggcc atggtttggg cctgccgcga catggtcagc
     3241 aagggatgat agcggggaat gtcgcggccc gacaagccgc aggccactta acagcacgct
     3301 gtcttcgact gattcaggcg agcccggcga cggcgcccgg ctcttccagc tccgagaagc
     3361 gcaccggcag cacatcgcgc gtggcgagct tgccgcttgc ggatttctcc accagcgtca
     3421 acgcctgctt ctccgggatg ccggtgggaa tgaccatgcg cccgccgggc ttgagctgtg
     3481 cgagcagcgg cggcgggatg agctcgcacg cagccgtcac gatgatattg tcgtaaggcg
     3541 cgtgctcggg aaggccgtag taaccgttcg ccacgcgcac ttcaatgttg gtatagccga
     3601 gccgcttgag acgcttcccg gcgccttccg cgagctcctg aatgatatcg acgctgtaga
     3661 cctgccg
//
LOCUS       DASURG010000048         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_828160, whole genome shotgun sequence.
ACCESSION   DASURG010000048 DASURG010000000
VERSION     DASURG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828160"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1075)
                     /locus_tag="VFY80_00775"
     CDS             complement(104..1075)
                     /locus_tag="VFY80_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006461.1"
                     /translation="MRFRWYGVAALLLLAMDAGAQAWAPQKNVEIVAGSAPGGSNDKT
                     ARAMERILGAQKLVPTTLTVINRPGGGGSIAYTYVAQRAGDPHYLAVAGSNILTGSIL
                     GSTTVTVQDFTPIAQLYEDYAVFAVQSGSPIRNGKELAERMKNPQKIVVGFANAFGSS
                     RHIAVGLLVKSMGSNVRDLKPVVYKGSAEAITSVLGGHIEAVVVGATNAAGHVANGRM
                     RVVAVAAPRRFSSGPLADAPTWREQGIDVVYGNWRQIYGPKGLTPAQLRYWEEALRKM
                     SDTQEWKADLEKNYWTPEFIVGDQLRAEIEREHKYLKTLLTELGLAK"
     gene            1215..>2080
                     /locus_tag="VFY80_00780"
     CDS             1215..>2080
                     /locus_tag="VFY80_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX6006462.1"
                     /translation="MNGAQSLVRTLVANGVNVCFTNPGTSEIHFVAALDKVDGMRCVL
                     GLFEGVVTGAADGYYRMTGRPACTLLHLGPGLGNGLANLHNAKKARSGIVNIVGEHAT
                     YHLKHDAPLTADIAGIARPVSHWVHTAGNARSLAGDCAAAIQQARHPPGQIATLIAPA
                     DTAWGEAEAHARAEPPPARNKVTGSVVADAARALKSGEPAMLLLGGAALRGGALEDAG
                     RIAAKTGCRLMSEYNNARAEGGAGRVRVDRLPYPVDQALATLKDVRQLVLAGAKVPVS
                     FFAYPGKPSVLIP"
BASE COUNT          343 a          710 c          679 g          348 t
ORIGIN      
        1 taccggcttg gtcggatagg gctgtgcggc gtaggcctgg accgccccgc acagcaggaa
       61 ggcggcgagc cagcgtgcag cgatcatcac aaatctccgc gctttatttt gcgagaccga
      121 gttcggtcag cagcgtcttc aggtacttgt gctcgcgctc gatctcggcg cgcaactggt
      181 cacctacgat gaactccggc gtccagtagt tcttctcaag atcggccttc cattcctgcg
      241 tatcggacat cttgcgcagc gcctcttccc agtaccgcag ttgagccggc gtgagaccct
      301 tcgggccata gatttgacgc cagttcccat agacgacgtc gatgccctgt tcgcgccacg
      361 tcggcgcatc ggcgagcggg ccgctcgaaa acctgcgcgg cgcggcgacg gcgacgaccc
      421 gcatgcggcc gttcgcgacg tgacccgcag cgttggttgc accgacgacg accgcttcga
      481 tatgaccgcc cagcaccgag gtgatcgcct ccgcggaacc cttgtaaaca acaggcttca
      541 gatcccgcac gttgctgccc atcgacttca cgagcaggcc cacggcgatg tggcggctgc
      601 tgccgaaggc atttgcgaaa cccaccacga ttttctgcgg gtttttcatg cgctccgcga
      661 gttcctttcc gttcctgatc ggagagccgc tctgcaccgc aaacaccgca tagtcctcgt
      721 acagttgcgc gatcggggtg aagtcctgaa ccgtgaccgt ggtcgagccc aggatcgaac
      781 cggtcaggat gttgctgccc gcgacggcca gatagtgcgg atcgccggcg cgctgcgcca
      841 cgtaagtgta ggcaatgctg cccccgccgc ccggccggtt gatgaccgtg agtgtggtcg
      901 ggaccagctt ctgcgcgccg agtatgcgct ccatcgcgcg cgctgtcttg tcgttgctgc
      961 ccccgggcgc cgagccggcg acgatttcga cgttcttctg cggcgcccac gcctgcgcgc
     1021 cggcatccat cgcgagcaac agcagggcgg ccacgccata ccagcggaat ctcatgagcg
     1081 gctcctccga aacgcgtcat gcgcgtggcg catcataagt cggccgcgac gggcgcgcca
     1141 agccgtgccg ctaccgtcca cgacgtgaac gcggtaacct tccgttcaaa cccatctgtc
     1201 cggagccttc tcccatgaac ggcgcgcaaa gcctcgtgcg cacgctcgtc gcaaacggcg
     1261 tgaacgtctg cttcaccaat cccggaacgt cggagatcca tttcgtcgct gccctcgaca
     1321 aggtggacgg catgcgctgc gtgctggggc tgttcgaggg tgtggtcacc ggtgcggctg
     1381 acggctacta ccgcatgacg ggccggccgg cgtgcaccct gctgcatttg ggtccggggc
     1441 tggggaacgg gctcgcgaac ctgcacaacg cgaagaaggc gcgctcgggc atcgtcaaca
     1501 tcgtcggcga gcacgcgacc taccatctga agcacgacgc gccgctcacc gcggatatcg
     1561 cggggattgc ccgcccggtg tcgcactggg ttcacaccgc gggcaatgcg cgtagcctcg
     1621 ccggggactg cgctgccgcg atccagcagg cgcgccatcc gccggggcag atcgcgacgc
     1681 tgattgcgcc ggccgacacg gcgtggggcg aggccgaagc gcacgcgcgc gccgagccgc
     1741 cgcccgcgcg caacaaagtg acgggcagcg tcgtcgccga cgccgcgcgc gcgctcaaga
     1801 gcggcgagcc ggccatgctg ctgctcggcg gcgcggcatt gcgcggcggg gcgctggaag
     1861 acgcagggcg tatcgcggcg aaaaccggat gccgcctcat gtccgagtac aacaacgccc
     1921 gcgccgaggg cggagcgggg cgcgtgcgcg tggaccgcct gccgtatccg gtggaccagg
     1981 cgcttgcaac actgaaggac gtgcgtcagc tcgtgctcgc aggcgcgaaa gtaccggtat
     2041 cgttcttcgc ctatcccgga aaaccgagcg tgctgattcc
//
LOCUS       DASURG010000049         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_419660, whole genome shotgun sequence.
ACCESSION   DASURG010000049 DASURG010000000
VERSION     DASURG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419660"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1843)
                     /locus_tag="VFY80_00785"
     CDS             complement(146..1843)
                     /locus_tag="VFY80_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX6006463.1"
                     /translation="MHSLQLSENTILMVFSVAVGAASALGVVAFYKLIDLAFAVFFRI
                     PSEQVSRSVFLAYRPIITALGLMTAWWIWQRFARMDQGMTVPDVQLAVARRGGYLPAR
                     PAVARTAASAVTLGSGGSAGSEGPVAVLGSAIGSWIGRIFDFDAGKVRVLVGSGAAAG
                     IAAAFNAPLAGAFFALEEILFAFSAAAFAPVVISSVVGALVSRAFFGNHPAFPIPEEY
                     GFALSSEIVVLYPLLGVICGVVAALFIRTHFRLQAAAERHRHHPLLLAGIGGAAVGVL
                     VYLSQGILVGYGHLAVRVEVFGRMAWYALALLAAGKILATSLTLNLGGSGGVFTPSLY
                     IGAATGGAFGAAVNQFVPNLTVAPAAYALVGMGAVVAAATDARITAILIVFEMTNDYA
                     IVPALMLVVAIASVVARKLEPYSLYSGHLHRLGEVLHRPPRRDVFASVRVADVLDPIE
                     PIAASATVDVLIERFATDGVTELPVINEEGRLLGIVGFAEVARVAREPDDGGRAIIAS
                     DLTAPTEIVIPQDTLLYAIEKMARRESGTLPVVNAQNGRLVGVITRRHILDAYERGT"
     gene            complement(1900..>2094)
                     /locus_tag="VFY80_00790"
     CDS             complement(1900..>2094)
                     /locus_tag="VFY80_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006464.1"
                     /translation="ADTWQAFEEADNVFDPTIARRLRQYILAPGNTTDRAEAYRQFRG
                     RDPRVEALLQVRGFPVGESN"
BASE COUNT          438 a          710 c          644 g          302 t
ORIGIN      
        1 gcgcacgtag gccagccacg gatagctcgg ctccaacagg tgcgcgccca caaagaccag
       61 ggtagccacc accagcagtc cggtcgccac ccgtttcatg cggcgcaaca gcagcagccg
      121 cgtggcgtcg tcgatcggtt cggtattagg tgccgcgctc atatgcatcc agaatgtggc
      181 gacgcgtgat gacaccgacc agtctgccgt tctgcgcatt cacgaccggc agcgtgccgc
      241 tttcgcgtcg ggccatcttt tcgatggcat agagcaatgt gtcctgtgga atcacgattt
      301 ccgtaggtgc ggttaggtcg gaagcaatga tggctcggcc gccgtcgtcc ggctcacgcg
      361 ccacacgcgc cacctcggcg aagcccacaa tgccgagcag ccgcccttcc tcattgatca
      421 ccggcaattc cgtcactcca tcggtggcaa agcgctcgat cagcacatcg acggtcgcac
      481 tcgccgcaat cggttcgatt ggatcgagca catcggccac ccgtacactg gcgaacacat
      541 cacgccgcgg cggccggtgc aacacttcgc ccagccgatg cagatgaccg ctgtagagcg
      601 aatacggctc gagcttgcgc gcgaccaccg acgcaatggc cacgaccagc atgagagccg
      661 gtacgatcgc gtagtcattc gtcatctcga aaacaatcag aatcgccgtg atgcgcgcat
      721 cggtcgcggc ggcgaccacc gcgcccatac cgaccagcgc atacgctgcg ggagctaccg
      781 tcagattggg tacgaactga ttcaccgcgg caccgaacgc gccgcccgta gccgcgccga
      841 tgtaaagcga gggtgtgaag acgccgccgg agccgccgag gttgagcgtg agcgacgtcg
      901 ccagaatctt gcccgcggcc agcagcgcca gcgcatacca cgccatccgg ccgaacactt
      961 cgacacgtac ggccaggtgg ccgtagccca ccagaatccc ctgggagaga taaaccagta
     1021 cccctacggc tgcgcccccg ataccggcca gtaacagcgg gtgatgccgg tgtcgctcgg
     1081 ccgctgcctg caaccggaaa tgtgtgcgaa tgaacagcgc ggccaccaca ccacagatga
     1141 cgccgagcag tggatacagc acgacgatct cgctggacag cgcgaagccg tactcctccg
     1201 ggatgggaaa cgcagggtga ttgccgaaga aagcgcgcga taccagcgcg cccacgacgc
     1261 tgctgatcac gacgggcgcg aacgccgccg cgctgaaggc aaacaggatt tcttcgagtg
     1321 cgaaaaaggc gccggcgagg ggtgcattga acgccgcggc gatgcccgcg gccgcacccg
     1381 agcccacgag tacgcgaacc ttgccggcat cgaagtcgaa gatgcgcccg atccacgagc
     1441 cgatggccga gccgagcacg gcgaccggac cctcgctgcc cgcagaaccg ccgctgccca
     1501 gcgtcacggc gctggccgcc gtgcgcgcca cggcaggacg cgcgggcagg taaccgccac
     1561 gccgggcaac ggccagctgc acatcgggca cagtcatgcc ctgatccatg cgcgcgaagc
     1621 gctgccagat ccaccacgcg gtcatgagac cgagggccgt gatgatggga cgataggcga
     1681 ggaagacgct gcgcgagacc tgctcactgg gaatgcggaa gaagacagcg aatgccaggt
     1741 cgatcagctt gtagaatgca accacgccca aagcggaagc ggcacccacc gcaaccgaga
     1801 agaccatgag gatggtgttc tcgctcagct gcaatgagtg cacgcctcgg acgacccgtt
     1861 cccacgaccg ccgtagctgc tgcaccggcg gcttaaccac taattcgatt cacccacggg
     1921 aaaaccacgc acctgcaata gcgcttccac gcgcggatcg cgtccccgga actgccgata
     1981 cgcttcggcg cgatcggtgg tgttgcccgg cgcgaggata tactgacgca gtctgcgcgc
     2041 gatggttgga tcgaatacgt tgtccgcttc ctcgaacgcc tgccaggtgt ccgc
//
LOCUS       DASURG010000050         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_419847, whole genome shotgun sequence.
ACCESSION   DASURG010000050 DASURG010000000
VERSION     DASURG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419847"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFY80_00795"
     CDS             <1..264
                     /locus_tag="VFY80_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6006465.1"
                     /translation="FRHGIPDGTEGTVKISIEQLPDTVRIEVADDGVGTKQTARSEVV
                     GSGRSIVDALARDELNGKFEFISTEHGTRAIVTFPRENARAAS"
     gene            complement(267..1031)
                     /locus_tag="VFY80_00800"
     CDS             complement(267..1031)
                     /locus_tag="VFY80_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6006466.1"
                     /translation="MDTKTYTVSQVSRMARVTVRTLHHYDDIGLLIPSQRSDAGYRLY
                     TDSDLQRLQQILLFRELGFALDAIAALLEQPAAERLSALVMQRARLEAELQQTSAVLR
                     AIETAIKNNEGGVSMSAEEFFEGMEDFNHAQYADEARERWGDTEAYQESVRRAKRYSK
                     DDWARMKEESEAITRSLADAMQAGKSADSLEATSLVEQHRLHIDRWFYPCSREFHVNL
                     GEMYVSDPRFTATYDKVRPGLARYIRDAIVANAGKE"
     gene            complement(1123..2487)
                     /gene="glnA"
                     /locus_tag="VFY80_00805"
     CDS             complement(1123..2487)
                     /gene="glnA"
                     /locus_tag="VFY80_00805"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6006467.1"
                     /translation="MRPSKETGPGRSRGLTRNDLLELMRAQQVRFLRLQFSDIMGIIK
                     NVEVPQTQFEKALAGDIMFDGSSIEGFVRVEESDMVLKPDYDTFAVFPWGDPENRVAR
                     LICDVYTADDRPFPGDPRWVLKRQIEAAARLGFQMMAGCEAEFFLFHRTADGSPSRST
                     HDSGGYFDLTPVDLGEVARRDIVNVLEMMGFEVETAHHEVAAGQHEIDFRYSDALTTA
                     DDLATFKFVVRNIALQHNLHATFMPKPVFGQNGSGMHTHQSLFKGEENAFFDASAPDQ
                     LSKVMRWYVAGLLRHARAFCAVTNPLVNSYKRLVPGYEAPVNVAWSHHNRSPLVRVPA
                     RRGKGTRLELRMPDPSANPYLALAVQLAAGLDGIRNKVNPPDPVDRNIWELSVRDRRK
                     YKIAELPRDLGEAVDLMKRSPFMKQVLGDHVYANFLAAKEQEWQEYIAQVHDWEIEQY
                     LALY"
     gene            complement(2502..3074)
                     /locus_tag="VFY80_00810"
     CDS             complement(2502..3074)
                     /locus_tag="VFY80_00810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6006468.1"
                     /translation="MGNIRLARPADAAEIRAIYAPIVRDTAISFEYEVPSVAEIERRL
                     ARVLAGRPWLVHVEAGRVLGYAYASPFRERAAYDWSVEVSIYVDSSVQRSGIGRRLYA
                     TLFDVLRALGYHQVIAGATMPNPASERLHRSLGFAQVAHFPAIGYKFGCWHDTVFWAL
                     PLRPTAGEAPAIININQLVKTAEWEWLTRD"
     gene            3311..>4290
                     /locus_tag="VFY80_00815"
     CDS             3311..>4290
                     /locus_tag="VFY80_00815"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX6006469.1"
                     /translation="MAQSTTTRRFDTLAAVKSWNPAGNAAGNNGNPRTPMDIEKIFGE
                     NTFGLAEMKARLPKPTYKALLATIESGAELDPSLADAVAIAMKEWALEKGATHFTHWF
                     QPLTGLTAEKHDSFVTPNVGGGAVAEFSGKDLVQGEPDASSFPSGGLRATFEARGYTA
                     WDPTSPAFIVEGPSGCYLAIPTAFASWAGDALDQKTPLLRSVHALSAQARRALKLFGV
                     EVNHVGATLGPEQEFFLIDQEFYYRRPDLVTTGRTLFGAKPPRGQELEDHYFGTIPER
                     VLCCMMEVERELYRLGVPVKTRHNEVAPGQFEMAPIYEQANIAADHQQLL"
BASE COUNT          838 a         1373 c         1293 g          786 t
ORIGIN      
        1 ttccgccacg gcattcccga tggcaccgaa ggaaccgtca agatcagcat cgagcagttg
       61 cccgataccg tgcgcatcga ggtggccgat gacggcgtcg gaacgaagca aaccgcgcgc
      121 tccgaagtcg tgggcagcgg ccggtcgatc gtggatgcgc tcgcgcgaga cgagctgaac
      181 ggcaagttcg agttcatctc caccgagcac ggcacacgtg ccatcgtgac tttcccgcgg
      241 gaaaacgccc gcgccgcttc ctgatcctat tctttgccgg cgttggccac gatggcgtcg
      301 cgtatgtagc gcgccagacc cggtcgcacc ttatcgtagg ttgccgtgaa gcgcggatcg
      361 ctcacgtaca tttcaccgag attgacgtga aactcacgac tgcacgggta aaaccagcga
      421 tcaatgtgca gacgatgctg ctccacgagc gacgtcgctt ctaaactgtc cgcagacttg
      481 cctgcctgca tggcgtccgc cagcgagcgc gtaatcgcct cgctttcctc cttcatgcgc
      541 gcccagtcgt ccttgctata gcgtttcgcc cgccgcacgg actcctggta ggcttccgta
      601 tcaccccaac gctcgcgcgc ttcatccgca tactgcgcgt ggttgaagtc ttccatgccc
      661 tcgaaaaatt cttccgcact catgctgacg cctccctcgt tgttcttgat ggccgtttcg
      721 atggcacgaa gcaccgcact cgtctgctgc agctccgctt ccagccgcgc gcgctgcatg
      781 accagtgcgc tgagccgctc ggcggcgggt tgctccagca acgcggcaat cgcatcgagc
      841 gcgaacccca gctcccggaa caacaggatc tgctgcagtc gctgcagatc ggagtcggta
      901 tacaagcgat agccggcgtc actgcgttgc gaggggatca gcagcccaat gtcatcgtag
      961 tgatgcagcg tgcgcacggt cacgcgtgcc atgcggctca cctggctgac ggtgtaggtc
     1021 ttcgtgtcca tggctgcaat ggtaggggat gacgtagcgt gagggtcaag gggttcagga
     1081 tcatgtcgaa aagcgacatg cggaaggcga cgccttccgc cgtcaataaa gcgccaaata
     1141 ctgctcgatc tcccaatcgt gcacctgcgc aatatactcc tgccattcct gctccttggc
     1201 cgccaggaag ttggcataaa cgtggtcgcc cagcacctgc ttcatgaacg ggctgcgttt
     1261 catcagatcg accgcctctc ccaaatcacg gggcagctcg gcgatcttgt acttgcggcg
     1321 gtcacgcacc gacagctccc agatgttgcg gtcgaccgga tcgggcggat tgaccttgtt
     1381 gcgaatgcca tccagtcccg cggccagctg caccgccaac gccaggtaag gattggccga
     1441 aggatccggc atgcggagtt ccagccgggt gcctttgccc cggcgggcag gcacgcgcac
     1501 cagcggtgag cgattgtggt gcgaccacgc gacattgacc ggcgcttcgt aaccgggtac
     1561 gagccgcttg taggaattca ctaatggatt ggtgaccgcg cagaaggcac gggcatgccg
     1621 cagcaggccg gccacatacc agcgcatcac cttcgacaac tggtccggag cactcgcatc
     1681 gaagaacgcg ttttcttcac ccttgaaaag cgattgatgc gtgtgcatgc ccgagccgtt
     1741 ctgtccgaac accggcttgg gcatgaaggt ggcgtgcagg ttgtgctgca gggcaatgtt
     1801 gcgcacgacg aacttgaagg tcgccaagtc atcggccgtg gtcagcgcgt cgctgtagcg
     1861 gaaatcaatc tcgtgttgcc ccgccgcaac ctcatgatgc gcggtctcga cttcaaaacc
     1921 catcatctcg agcacgttca cgatgtcacg acgcgcaact tcgcccaggt cgaccggcgt
     1981 caggtcgaag tagcctccgg aatcgtgtgt gctgcgtgaa ggcgagccgt ccgccgtccg
     2041 gtgaaacaga aagaactcgg cttcacaccc cgccatcatc tgaaacccga gcctggccgc
     2101 ggcttcaatc tgccgcttca acacccagcg aggatcgccc gggaaaggac gatcatcggc
     2161 ggtgtagacg tcgcaaatca gccgggctac gcgattctcc ggatcccccc acggaaaaac
     2221 cgcaaacgtg tcgtaatccg gcttcaacac catatccgac tcttccacac gcacgaagcc
     2281 ttcaatcgaa gaaccgtcga acatgatgtc gcccgccagt gctttctcga actgcgtctg
     2341 cggcacttcc acgttcttga tgatgcccat gatgtcggag aactgcaggc gaagaaaacg
     2401 cacctgctgc gctcgcatca gctcgagcaa atcgttgcgc gtcagaccgc ggctgcgtcc
     2461 ggggcccgtt tccttcgaag gccgcacttt gtgctcagat actagtctct tgtcagccat
     2521 tcccactcgg cggtcttaac cagctggttg atgttgatga tggcgggcgc ctccccggcc
     2581 gtcggccgca gcggcaaggc ccagaaaacc gtgtcgtgcc agcagccgaa cttgtagccg
     2641 atggcgggga aatgagcgac ctgcgcaaag cccaggctgc gatgcagccg ctcactcgcg
     2701 ggattgggca tggtcgcgcc cgcgatcacc tgatggtagc cgagcgcgcg cagcacgtcg
     2761 aacagcgtcg catacaaccg ccgcccaatg ccgcttcgct gcaccgagct gtcgacataa
     2821 atcgacactt ccacgctcca gtcgtaggcc gcccgctcac ggaacgggga agcataggcg
     2881 tacccgagca cgcgcccggc ctccacgtgg accagccagg gccggccggc cagaacacgg
     2941 gccaggcgac gctcgatctc ggcgaccgaa ggcacctcat actcgaagga gatggcggtg
     3001 tcgcgcacga taggggcata aatggcccgg atttcggcag cgtccgccgg cctggccagc
     3061 cggatgtttc ccatgccggg atcgtagggt gggacggggc cggctgtcaa ttgaaatgtt
     3121 gccaatcggc aacttctgat gtaacatctc gcccgtctgg caccattttc gcaaaattcg
     3181 tccactgaag atggcctcct ggccacttta catgatacac aaccccggcc cgcccggacg
     3241 gccaacgacg gcccccgaaa tgcaccggag accacacacc atcagcccag gccctggccc
     3301 cgaggagcgt atggcgcaat ccacgaccac ccggcgtttc gacacgctgg ccgcagtcaa
     3361 gagctggaat ccggcgggca atgccgccgg caacaatggc aacccgcgca caccgatgga
     3421 catcgagaaa atttttggtg agaacacgtt cgggctggcg gagatgaagg cgcgtcttcc
     3481 gaagccgacc tacaaggcct tgctggccac gatcgagagt ggtgcggagc tcgatccatc
     3541 gctggcggac gcagttgcca tcgccatgaa ggagtgggcg ctggagaaag gtgcgacgca
     3601 cttcacccac tggttccagc cgctcacggg gctgacggcg gaaaagcacg actccttcgt
     3661 cacgcccaac gtgggtggcg gtgcggtcgc cgaattcagc ggcaaggacc tggtgcaggg
     3721 ggagccggac gcatcgtcat tcccgtcggg cgggttgcgt gcgacgttcg aagcgcgcgg
     3781 ctacacggcg tgggatccga cgtcgccggc gttcattgtt gaaggtcctt ccggctgcta
     3841 tctggccatc ccgacggcat tcgcatcgtg ggcgggggat gcgctcgatc agaaaacgcc
     3901 gctgttgcgc tcggtgcacg cgctttccgc gcaggcgcgc cgtgctctga agctctttgg
     3961 ggtggaggtc aatcatgtgg gagccacgct tggtccggaa caggagtttt tcctgatcga
     4021 tcaggagttc tactaccggc gccccgacct ggtcaccacc ggtcgcacgc tcttcggtgc
     4081 caagccgcct cgcgggcagg agctggaaga tcactacttc ggcaccattc ccgaacgggt
     4141 gttgtgctgc atgatggagg tcgagaggga gctgtatcgg ctgggcgtac cggtcaagac
     4201 acgccacaac gaagttgcgc ccgggcaatt cgagatggcc ccgatctatg agcaggccaa
     4261 catcgcagcg gaccatcagc agctgctgat
//
LOCUS       DASURG010000051         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_522296, whole genome shotgun sequence.
ACCESSION   DASURG010000051 DASURG010000000
VERSION     DASURG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522296"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VFY80_00820"
     CDS             complement(<1..1351)
                     /locus_tag="VFY80_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX6006470.1"
                     /translation="MYGTLSTPTPAHARRVLPLTFAAIGVVFGDIGTSPLYAFREAFS
                     GHHPLAVTEGHVLGVLSMIFWAVTLVVIVKYLLFVLRFHNKGEGGVLALLAYAGRLTR
                     ENPRLQFFTAMIAIFAASLFFGDAVITPAISVLSAVEGLAVATPALEHWIVPATMVII
                     VWLFTIQRGGTSRIGRYFGPITTLWFVTIAILGAASIAQTPGVLAAIDFRHAAGFALE
                     SPLAAFLALGAVFLAITGAEALYADMGHFGAGPIRIGWFGLVFPSLMLNYFGQGALVL
                     RDPATARNPFYLLAPDMLLIPLIVLATAATVIASQATISGAFSVTQQASRLGYMPAFR
                     ITHTSESERGQIYLPQMNWAMLVIVVLLVLGFGSSSNLAAAYGIAVSGTMLLETMLVA
                     VVAWALLPRLRVAIAVGVAIVALTELMFLASNATKIVAGGWFPIVCGLAMFTVLTTWK
                     "
     gene            complement(1417..>2059)
                     /locus_tag="VFY80_00825"
     CDS             complement(1417..>2059)
                     /locus_tag="VFY80_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX6006471.1"
                     /translation="AKPVKGMLTGPVTLLQWSFVRDDQPRHATALQLALAMRDEVFDL
                     EAAGICIIQIDEPALREGLPLRRKEWDAYLDWAVRAFRLTASIASEETQIHTHMCYAE
                     FSDILPAIAAMDADVVTIEASRSKMMLLESFRNFDYPNGIGPGVYDIHARRVPQVGEM
                     VRLLEQAAAVIPVERLWANPDCGLKTRGWKETEAALRNMVAAARNVRDRHARM"
BASE COUNT          408 a          667 c          681 g          303 t
ORIGIN      
        1 gcttccacgt cgtcagcacg gtgaacatcg cgagcccgca cacgatcggg aaccagccgc
       61 cggcgacgat cttcgtggcg ttcgacgcga gaaacatcaa ttccgtcagc gcgacgatcg
      121 cgacaccgac cgcgatggca acccgcaggc ggggcagcag cgcccacgcg accaccgcga
      181 cgagcatcgt ctcgagcagc atcgtgcccg ataccgcgat accataggcg gccgcaaggt
      241 tggacgaaga gccgaagccg agcaccagca gcacgacgat caccagcatc gcccagttca
      301 tctgcggcag gtagatctgg ccgcgctcgg actcggaagt gtgcgtgatg cgaaacgccg
      361 gcatgtagcc gagccgcgag gcctgctggg tgaccgagaa cgcccccgag atcgtcgcct
      421 gcgacgcgat gacggttgcc gccgtggcga gcacgatgag cgggatcagc aacatgtcgg
      481 gcgcgagcag gtaaaacggg ttgcgcgccg tggctggatc gcgcagcacc agcgcgccct
      541 ggccgaaata gttcagcatg agcgacggaa agacgagccc gaaccagccg atccggatcg
      601 gccctgcgcc gaagtgtccc atgtccgcgt agagcgcctc ggcgccggtg atggcgagaa
      661 acaccgcgcc gagcgcgaga aacgcggcaa ggggcgattc gagcgcgaag cccgcggcat
      721 gccggaaatc gatcgcagcg agcacgcccg gcgtttgcgc gatgctcgcc gcgcccagaa
      781 tggcgatcgt gacgaaccac agcgtggtga tcgggccgaa gtagcggccg atgcgactgg
      841 tgccgccgcg ctggatcgtg aacagccaca cgataatcac catcgtggcc ggcacgatcc
      901 aatgttcgag cgccggcgtc gcaaccgcca agccctccac cgcggacagc accgaaatcg
      961 ccggcgtgat caccgcgtcg ccgaaaaaga gcgaagcggc gaagattgcg atcatcgcag
     1021 tgaagaactg cagccgcgga ttttcgcgcg tgaggcggcc cgcgtacgcg aggagtgcca
     1081 gcacgccacc ctcacccttg ttatggaatc gcagcacgaa gaggaggtac ttcacgatca
     1141 ccacgagcgt aacggcccag aagatcatcg agagcacgcc gagcacgtgc ccttcggtca
     1201 cggcaagcgg gtggtgtcca ctgaacgctt cgcggaatgc atagagcggg cttgtgccga
     1261 tgtcgccgaa gacgaccccg atggcggcga aggtgagcgg aagtacgcgc cgggcatgtg
     1321 cgggcgtggg tgtggacaag gtgccataca tggcggacgg ctccggcggc tgatgcgcca
     1381 gcgcgaatta taaccgtcgc ccaccgccga tgagcgtcac atgcgcgcgt ggcggtcgcg
     1441 cacgtttcgg gccgccgcga ccatgttgcg cagcgccgct tcagtctctt tccagccgcg
     1501 cgttttcagt ccgcagtcgg gattggccca cagccgctcc acggggatca cggcggcggc
     1561 ctgctcgagc aaccgtacca tttcgccgac ctgcggcacg cgccgcgcgt ggatgtcata
     1621 aacgcccggg ccgatgccgt tgggatagtc gaaattgcga aatgactcga gcagcatcat
     1681 cttcgagcgc gatgcctcta tcgtcacaac gtccgcgtcc atcgccgcga tcgccggcag
     1741 aatgtcgctg aactcggcgt agcacatgtg ggtgtggatc tgcgtttcct cggacgcgat
     1801 ggacgcggtg agccgaaacg cacgcacggc ccagtcgaga taggcgtccc attccttcct
     1861 tcggagcgga agcccctctc gcagcgccgg ctcgtcgatc tggatgatgc aaatgccggc
     1921 tgcttcaaga tcgaagactt catcgcgcat cgcgagggcg agctgcagcg cggtcgcgtg
     1981 gcgcggctgg tcgtcgcgca caaacgacca ttgcagcagg gtaaccggtc cggtcagcat
     2041 gcctttcacc ggtttggcc
//
LOCUS       DASURG010000052         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_12182, whole genome shotgun sequence.
ACCESSION   DASURG010000052 DASURG010000000
VERSION     DASURG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12182"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="glgP"
                     /locus_tag="VFY80_00830"
     CDS             complement(<1..776)
                     /gene="glgP"
                     /locus_tag="VFY80_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX6006472.1"
                     /translation="MISPFIHSAHIGYFSMEIALAPDIPTYSGGLGVLAGDTLRSAAD
                     LRLPLVGVTLVSRMGYFRQEIDGAGQQNEQAASWNPAEKCEPLEAKIVVNIMGRKVWV
                     GGWLYVVKSHLGGTLPVILLDTDLPENDRADREITHYLYGGDQAYRLKQEIVLGIGGV
                     RMLLALGLQVRKYHMNEGHSALLAVELMRRFELFNRRRPHEPTYHLPAVRAQCIFTTH
                     TPVEAGHDQFDWSLVQEILQDYIALDEFKKLAGEDRLNMTR"
     gene            954..2048
                     /locus_tag="VFY80_00835"
     CDS             954..2048
                     /locus_tag="VFY80_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6006473.1"
                     /translation="MKISRIETRVVRLPAEEPLAGGPASPGATRDFVTLRVATDDGVE
                     GLGVTFFGAALTPALKSAVDSLGALAIGEDPICVEAVTGKLRAAAGSAGPAGIFTLAL
                     AAVDIALWDIKGKVLNLPVARLAGGYRDRVPTYASGALMRTFPLDHVVRAADALVEKG
                     YRQMKTQLALPGETTPEREVERIRLIREAIGPDIDLMCDINQRWDVRQAISIGSRLEQ
                     YHLYWLEDVVASDDHPGLAAVASALATPIAAGEYVYGKVPFRHMLEARAVDIVMIDVL
                     RAGGITQWLKIAGMAEAFNLPVVNHLYPEVSVHLVAAIPNGLTVEYMPWSLRLFRDVP
                     VPVGGELKVPDKPGLGLEFDETVVRRYGVD"
     gene            complement(2053..>2596)
                     /locus_tag="VFY80_00840"
     CDS             complement(2053..>2596)
                     /locus_tag="VFY80_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX6006474.1"
                     /translation="ADFLPESLYLDAIKAHSDVVRPFATEQQATLTVNPYHLGFSMWE
                     HIVESHGIEQARRICREEDDFGFIRNYLDRDLAKKLGLFVFEAKSGGEIRITGRGIDA
                     IREAILAPKFNYGAPRIAVSELRTDGTLVLVHDHASDGRGLDLSRAARVLDYVHRVWR
                     RPVILHTADARGEPQELHKQ"
BASE COUNT          457 a          823 c          832 g          484 t
ORIGIN      
        1 cgcgtcatgt tgagccgatc ttcaccagcg agcttcttga attcgtcgag cgcgatgtag
       61 tcctgcagga tttcctgcac caggctccag tcgaactggt cgtgacccgc ttcgaccggc
      121 gtgtgcgtcg tgaagatgca ttgggcgcgc accgcgggca ggtgataggt cggctcgtga
      181 ggtcgccgcc gattgaacag ctcgaagcgg cgcatcaact ccacggcgag gagcgcggaa
      241 tgcccttcgt tcatgtggta cttgcgtacc tgaaggccca gcgcgagcag catgcgcacc
      301 ccgccgattc cgagcacgat ctcctgcttc agtcggtagg cctgatcgcc gccatagaga
      361 taatgcgtga tttcgcggtc ggcccggtcg ttctccggca aatcggtgtc gagcagaatc
      421 accgggaggg tgccgcccag atgacttttc acgacgtaaa gccagccgcc cacccaaacc
      481 ttgcgtccca tgatgttgac gacaatcttg gcttcgagcg gctcgcactt ttctgcagga
      541 ttccatgagg cggcctgctc gttctgctgg ccggcgccgt cgatctcctg cctgaagtat
      601 cccatccggc tgaccaacgt tactccgacg agcggcagac gcaggtccgc ggccgagcgt
      661 agcgtgtcgc cagccagcac gcccagcccg ccgctgtagg tcggaatatc gggcgcgagc
      721 gcgatctcca tcgagaagta accgatgtgc gcggaatgga tgaagggcga aatcaaaagg
      781 aacctcccgc aaactaaccc gtaagcttac cagcagcgga gcgttgcgcg gtagccactt
      841 ttcggttttt gcgccacatc gcgttcgttc gccttaccct gcggtgatgg tctcgctcga
      901 gcgcactgat caggtatagt gcgtcttccc ttcacccggc ccagcgctgt gccatgaaaa
      961 tctctcgtat cgaaacgcgc gtggtgcggc tccccgccga agagcctctt gccggcgggc
     1021 ctgcatctcc aggtgcaacg cgcgacttcg tcacgctgcg ggttgccacc gacgacggcg
     1081 tcgagggtct gggcgtcact tttttcgggg cggcgcttac cccggcgctc aagtccgccg
     1141 tcgacagcct gggcgcgctc gcgatcggcg aagacccgat atgcgtcgaa gcagtcacag
     1201 gcaagctgcg ggcggcagcg ggcagcgcgg gccccgcggg catatttact ctcgcgctcg
     1261 cggccgtcga catcgcgctt tgggacatca agggcaaggt gctgaacctg ccggttgccc
     1321 gtctcgcggg aggctatcgc gatcgagtgc ccacctatgc gagcggagcg ctcatgcgca
     1381 cctttccgct cgatcacgtg gtgcgtgcgg cggacgcgct ggtcgaaaaa ggttatcggc
     1441 agatgaagac acaactcgcc ctgccgggcg agacgacacc cgagcgcgag gtggagcgca
     1501 tccgcctcat acgcgaagcg atcggacccg acatcgacct gatgtgcgac atcaatcaac
     1561 gctgggatgt gcgccaggca atctcgatcg gcagccgcct ggagcaatac catctctact
     1621 ggctggaaga cgtggtcgca tccgacgacc accctggact cgcagccgtc gcttcagcgc
     1681 tcgccacgcc gatcgctgcg ggcgagtacg tgtatggcaa ggtgccattt cgccacatgt
     1741 tggaggcgcg cgcggtcgat atcgtcatga tcgacgtgct gcgcgcgggc ggtatcacgc
     1801 agtggctcaa gatcgcaggc atggccgaag cgttcaacct gccggtcgtc aaccatctgt
     1861 acccggaagt gtcggtgcac ctcgtcgcgg cgattcccaa cgggttgacg gtggagtaca
     1921 tgccctggtc gctgcggctc ttcagggatg tgcccgtgcc ggtcggaggc gagctcaagg
     1981 tgcccgacaa acccgggctc ggcctcgagt tcgacgagac ggtggtccgc cgctacgggg
     2041 tggactgaag cgctattgct tgtggagttc ctgcggctcg ccgcgcgcgt cggcggtgtg
     2101 caggatgacg ggacgacgcc atacgcggtg gacataatcg agcacgcgcg ccgcgcggct
     2161 caggtcgagc ccgcgaccat cgctcgcatg atcgtgcacg agcacgagcg tgccgtcggt
     2221 gcgcagctcc gacaccgcga tccggggggc gccgtagttg aacttcggcg cgaggatcgc
     2281 ttcacgtatg gcgtcgatgc cgcgcccggt gatcctgatt tcgccgccgc tcttggcttc
     2341 aaagacgaaa agcccgagtt tcttcgctag gtcgcgatcg aggtagttcc ggatgaagcc
     2401 gaaatcgtct tcctctcgac agatgcgccg cgcctgctcg atcccgtgcg actcgacgat
     2461 gtgctcccac atcgagaacc cgaggtgata cggattcacc gtaagcgtcg cctgctgctc
     2521 cgtggcaaag ggccgcacga cgtcggaatg cgccttgatg gcgtcgagat agagcgactc
     2581 gggcagaaaa tcggct
//
LOCUS       DASURG010000053         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1033059, whole genome shotgun sequence.
ACCESSION   DASURG010000053 DASURG010000000
VERSION     DASURG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033059"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFY80_00845"
     CDS             <1..461
                     /locus_tag="VFY80_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6006475.1"
                     /translation="ATSEPATRAHVVSQFDYVRTAHRFEGGNPNFLGIRVLRYGADFI
                     SAIGLSQIEQRVRELTTTCLALLRQAGLRTQTPEEWEERAHIVNVLVPDAAGLMERLR
                     ARHRVVTNVKDGALRLSMSFFNNEQDLERAVHAIQRELAGTAGATAVSIT"
     gene            538..1440
                     /locus_tag="VFY80_00850"
     CDS             538..1440
                     /locus_tag="VFY80_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6006476.1"
                     /translation="MSWKFELLMKPSAVPLTEGPVWDGEALLFTHIRASRIMRYDPKS
                     GALGEWRTGTNRTNGLAFDADGRLFGCCSGGRSIVRFDPAGKIVTVADRVDGQRLNTP
                     NDLAIDRKGRIWFTNPWNEGNIDPTEKQELDNRSVLRADPQKDGSYTVTRVTFDTTQP
                     NGILVSQDQSTLYVAESGYDTKIPRELRAYPIREDGSLAAYSTLFTWGADVRGLHRGI
                     DGMCLDRDGNIVATAGWEVNGAGPMIYVFTPRGRVLETHPVPANRPTNCCFGGPDLTT
                     LFVTTTQGHFFKAHTDRVGWAMYP"
     gene            complement(1454..2437)
                     /locus_tag="VFY80_00855"
     CDS             complement(1454..2437)
                     /locus_tag="VFY80_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX6006477.1"
                     /translation="MEHPGGLEYSVVEGWEQIPRGYMHRDVAGVAVDGEDRVYLICRG
                     DHPVMVYDRNGNFLRSWGEGEFTYRTHGISVGPNGTIFCTDDGNHTVRQFTPEGRLLM
                     TLGTMNTPSDSGYDGKNLDTIKRGAGPFNRPTNIAVGPKGDLYVSDGYGNARVHQFSP
                     TGQLKRSWGSPGRGPGEFHLPHGIAVASDGRVFVCDRESDRIQIFSPDGEYLEEWTDT
                     QRPTHLVFDAHGRAYVTELWWHEGDKSYTHGPITKARHARVSIFDRDGTLLARWGTPE
                     ATAAGSFAAPHGLALDSRGDLYVSEVTWTFAVSRGKAPADCHTFQKFALKR"
     gene            complement(2895..>5379)
                     /locus_tag="VFY80_00860"
     CDS             complement(2895..>5379)
                     /locus_tag="VFY80_00860"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX6006478.1"
                     /translation="MRILAEQFVLAETIEDAMARSRARRPYLFSYDMLGEAARTADDA
                     ANYFASYRRALEAIEVPDSISVKLSALHPRFEESKRERVFSELLPRLQSLCEIAAARG
                     VGLTIDAEESERLELTLDLFDALVPDSRIGLAVQAYQKRALAVCEWLASLKRPVSVRL
                     VKGAYWDSEVKRAQQLGMPDYPVFTRKCATDVSYVACATRLLSAAHVHPAFATHNCRT
                     VATILEMTHEAMRGRRGSERYEFQKLLGMGDALYDALLAERSVPVRVYAPVGSFRDLL
                     PYLVRRLLENGANTSFVHQIADPAVPVAQLVADPVATLPEPYTPDPRIPLPRNLYPDR
                     LNSIGIDLSVRANLSELHDRVVADRERILAPVEETSPASLDAMLSRAETAFESWSRTP
                     AAARALVLERTADALEAQQVDLVSLIVREGRRTYGDAVSEVREAADFCRYYAAQARAK
                     FASPQTLPGPAGERNTLSLHGRGVFACVSPWNFPLAIFTGQVAAALAAGNTVIAKPAE
                     QTPRIGARAISIFRACGLPEEVAACAIGDGERVGAPLVADARIRGVAFTGSVDTAQHI
                     SRALAARNGPIVPLIAETGGVNAMIVDASALPEQVVDDVVISAFHSAGQRCSAQRILF
                     VDEGCAARVMTLVAGALAELKVGDPAEPDTDIGPIIDAPSHATLQRHVEGLRKSARVI
                     GEAPLDPRIADADCYLAPIAFELSIDALPRTEIFGPVLHVVMYRRSDLGRVLEWIRAT
                     GYGLTLGIHSRIEGFVDDVVRAARVGNVYVNRSMIGAVVGVQPFGGEGLSGTGPKAGG
                     PNYLLRFATERTLSVNTAAVGGITELLSK"
BASE COUNT          969 a         1784 c         1732 g          894 t
ORIGIN      
        1 ccgcgacctc ggaaccggcg acgcgggcgc acgtcgtaag ccagttcgac tacgtgcgca
       61 ccgctcaccg cttcgaaggg ggcaacccca actttttggg catccgcgtg ttgcgctacg
      121 gcgcggactt catcagcgca atcggtcttt cgcagatcga gcagcgggtg cgcgagctca
      181 cgacaacatg ccttgcgctc ctgaggcagg cagggctgcg cacgcagacg cccgaagagt
      241 gggaagaacg cgcgcatatc gtcaacgtgc tcgtcccgga tgccgccgga ctcatggagc
      301 ggctgagggc gcgccatcgc gtcgtcacca acgtcaagga cggcgcgctg cggctgtcga
      361 tgagcttctt caacaacgag caggacctcg agcgtgccgt gcatgccatc cagcgtgaac
      421 tcgccggcac agcaggcgct accgccgttt cgatcacttg agccgttccg gcgaacgccg
      481 ggaatcccca cgaccggatg ccggtttcac tcggcacgga tcagcgaagg agacgccatg
      541 agctggaaat tcgagctgct gatgaagcct tccgccgtgc cgctcaccga aggcccggta
      601 tgggacgggg aggcgctgct cttcacccac atccgcgcga gccgcatcat gcgctacgac
      661 ccgaaaagcg gcgcgctcgg cgagtggcgc accggcacca atcgcaccaa cgggctcgcc
      721 ttcgatgccg acggccgact ttttggctgc tgttcaggcg ggcgttcgat cgtacgtttc
      781 gatcccgccg gcaagatcgt caccgttgcc gaccgtgtag acggccagag gctcaacacg
      841 ccgaacgatc tcgcgatcga ccgcaagggt cgcatctggt tcaccaatcc gtggaacgag
      901 ggcaacatcg acccgacgga gaagcaggag ctcgacaatc gctccgtgct ccgtgccgac
      961 ccgcagaagg acggcagcta tacggtcacg cgcgtcacct tcgacacgac gcagccgaac
     1021 ggcatcctcg tttctcaaga ccagagcacg ctttacgtcg cggaaagcgg ttacgacacg
     1081 aaaataccgc gcgagcttcg cgcctatccg atacgcgaag atggcagtct ggccgcatac
     1141 tcgacgctct tcacctgggg cgccgatgtg agagggctgc atcggggcat cgacggcatg
     1201 tgcctcgatc gcgacggcaa catcgtcgcg accgcgggat gggaagtgaa cggggcggga
     1261 ccgatgatct acgtcttcac gccgaggggc cgcgtgctcg agacgcatcc tgtgccggcg
     1321 aatcgcccca ccaactgctg ctttggcggc cccgatttga cgacgctatt cgtcaccact
     1381 acgcaggggc acttcttcaa ggcgcatacc gaccgtgtcg gctgggcgat gtacccctga
     1441 gccgggcgga atatcagcgc ttcagggcga atttctggaa cgtgtggcag tccgccggcg
     1501 ccttgccgcg gctcacggcg aacgtccacg tcacttcgct cacgtagagg tcgccgcgcg
     1561 aatcgagggc gagcccgtgt ggcgcggcga agctgccggc ggcagtcgct tcgggcgtcc
     1621 cccagcgcgc gagaagcgtg ccgtcccggt cgaagatgct cacccgggcg tggcgtgcct
     1681 tcgtgatcgg cccgtgggtg tacgacttgt cgccctcgtg ccaccagagt tcggtcacgt
     1741 acgcgcgtcc gtgcgcatcg aatacgaggt gcgtcggcct ttgagtatcc gtccattcct
     1801 ccagatattc gccgtcgggg ctgaagatct ggatgcggtc gctctcgcga tcacacacga
     1861 agacgcggcc gtcggacgcc accgcgattc cgtgcggaag atggaactcg cccggtccgc
     1921 gtcctggcga tccccacgag cgcttcaact gtcccgtggg cgaaaactgg tggacacgcg
     1981 cgttgccgta accatcggag acgtaaaggt ctcccttcgg gccgaccgcg atgttggtag
     2041 gacggttgaa cggcccggcc ccgcgcttga tggtgtcgag attcttcccg tcgtagccgg
     2101 agtccgaagg ggtgttcatg gtgcccaggg tcatgagcag cctcccctcg ggcgtgaact
     2161 gccgcaccgt gtgattgccg tcgtcggtgc agaaaatcgt gccattgggg ccgaccgaga
     2221 tgccgtgggt gcgataagtg aattctcctt cgccccatga gcgcaggaag ttgccgttcc
     2281 ggtcgtagac catgacggga tggtcaccgc ggcagatgag atacacgcgg tcctcgccgt
     2341 ccaccgccac cccggccaca tcgcggtgca tgtaaccccg cggaatctgc tcccagcctt
     2401 cgacgacgga atattcgaga cctcccgggt gttccatgtt tcctccaagt tcgcgaccgt
     2461 atacggaccg ccatgatgcc atcgccacga cacgaccgca cgcgcctcgt gacaacccgc
     2521 actggcagtc tgcatcggtt gcagagcgcg gaatacgttt tccaggaaaa aagcatgaag
     2581 aatttacgtc ctacgcgagc gctgctcgcg gtgacctgcg actttcgtca agctcgtgac
     2641 gggacggcca gtgcatctcg tgctcagcga tcgcacgatg gagttcgacg acagcggccg
     2701 ctgcgtagcg gggtgctgag cgcagcgcgc atcctggtct ttcctggatt ccggatcatt
     2761 gtccggaatg acgatactgc gggcgccatg gcgaggctgc gtatcccgga atgacgacag
     2821 tgaggtccgc aacgacgtcg caccggcgaa agccggtgtc caggcttccg tagggccggc
     2881 ttcgattctg gctcctattt actcagcaac tcggtgatgc cgccgaccgc ggcggtattg
     2941 acgctcagcg tcctttcggt cgcgaaccgc agcagataat tcggtccgcc ggccttgggc
     3001 cccgtgccgg agagcccttc gccgccgaac ggctgcacac cgacgaccgc gccgatcatg
     3061 ctgcggttga catagacgtt tcccacgcgc gctgcgcgca ccacgtcatc gacgaatcct
     3121 tcgatgcgcg aatgtatgcc gagcgtgagc ccatagccgg tggcgcggat ccactccagt
     3181 acacgaccaa gatcgctgcg gcgatacatc accacgtgga ggacgggccc gaagatttcg
     3241 gtgcgcggca acgcatcgat ggaaagctcg aaggcaatcg gcgcgagata gcagtccgca
     3301 tcggcgatgc gtggatcgag cggggcttcc ccgatcaccc gcgccgattt gcgcagtccc
     3361 tcgacgtggc gttgaagcgt ggcgtggctc ggagcatcga tgatgggccc gatgtcggta
     3421 tcgggttcgg ccggatcgcc cactttgagc tccgccagcg cgcccgcgac gagtgtcatc
     3481 actcgcgctg cgcagccctc gtcgacgaag agaatgcgct gcgcggagca gcgctggccc
     3541 gcgctgtgga acgccgagat caccacatcg tccaccacct gctccggtag tgccgaagca
     3601 tcgacgatca ttgcgttgac gccgccggtt tcggcaatca gcggaacgat cggcccgttg
     3661 cgcgcagcca atgcccggct gatgtgctgc gcggtgtcga ccgatccggt gaatgcgacg
     3721 ccgcgtatcc gggcatcggc gacgagcggc gcgccgacgc gctcgccatc cccaatggcg
     3781 cacgccgcca cctcctccgg cagcccgcac gcccggaaga tcgagatcgc gcgcgccccg
     3841 atacggggtg tttgctccgc gggtttcgcg atcacggtat tgcccgcagc gagcgccgcc
     3901 gcgacctgac cggtgaaaat cgccaacgga aaattccacg gactcacgca cgcgaagacg
     3961 ccgcggccgt gcagggacag cgtgttgcgc tcgccggccg gacccggcag cgtctgcggc
     4021 gatgcgaatt tcgctcgcgc ctgcgccgcg taatagcgac agaagtccgc cgcctcgcgc
     4081 acctcggaca ccgcatcgcc ataggtgcgc cggccctcgc gcacgataag cgaaacgaga
     4141 tcgacctgct gcgcctccag ggcatcggcg gtcctctcaa ggaccagcgc gcgcgcggcc
     4201 gccggcgttc gcgaccagga ctcgaacgca gtctccgcac gtgagagcat ggcgtcgagc
     4261 gaggcgggag aagtctcttc cacgggagcg agaatgcgct cgcggtcagc cacgacgcgg
     4321 tcgtgaagct ccgagaggtt cgcccggacc gaaagatcga tgccgatgga attgaggcgg
     4381 tcggggtaga gattgcgcgg gagcgggatg cgcggatcgg gcgtataggg ctccggcaac
     4441 gtcgcgacgg gatcggcgac gagctgcgcg actggcacgg cgggatcagc aatctggtga
     4501 acgaacgagg tgttggcgcc gttctccaga agccgccgca cgagatacgg caggagatcc
     4561 ctgaagcttc ccaccggcgc atacacgcgt accggcacgc tcctctcggc gagcagcgcg
     4621 tcgtagagcg catcgcccat cccgaggagc ttctggaatt cgtagcgctc gctgccgcgc
     4681 cggccgcgca tcgcctcgtg cgtcatttcc agtatggtcg ccaccgtgcg acagttgtgc
     4741 gtggcaaagg caggatggac gtgcgccgcg gaaaggagtc gcgtcgcgca cgcgacgtag
     4801 ctcacatccg tcgcgcactt acgcgtgaat actgggtagt cgggcatgcc cagctgctgt
     4861 gcgcgcttca cctcgctgtc ccagtacgcg cccttcacca gccgcacgct gacgggccgc
     4921 ttcaggctcg caagccactc gcacacggcc agcgcgcgct tctgataagc ctgcaccgcg
     4981 agcccgatcc gcgaatcggg cacgagtgcg tcgaagaggt cgagcgtgag ctcgagccgc
     5041 tccgactcct cggcatcgat agtgagtcct accccgcgcg ccgctgcaat ctcgcacagc
     5101 gattgaaggc gcggcaacag ctcggaaaac acgcgctcgc gcttgctctc ctcgaagcga
     5161 ggatgaagcg cggaaagctt gaccgagatc gaatcgggca cctcgatcgc ctccagggcg
     5221 cgccgatacg aggcgaagta gttcgccgcg tcatcggccg tgcgcgccgc ctcgcccagc
     5281 atgtcgtagg aaaagagata gggccgccgt gcccgactgc gcgccatcgc gtcctcgatg
     5341 gtttctgcca gtacgaactg ctcggccaga atccgcatc
//
LOCUS       DASURG010000054         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_114474, whole genome shotgun sequence.
ACCESSION   DASURG010000054 DASURG010000000
VERSION     DASURG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114474"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /gene="ribA"
                     /locus_tag="VFY80_00865"
     CDS             <1..1235
                     /gene="ribA"
                     /locus_tag="VFY80_00865"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX6006479.1"
                     /translation="YIVERVLPDVRRGRMVILVDSEDRENEGDLFVAAERATPEAINF
                     MAQHGRGLISLALTEERMRALGLALIADDQINTTKFQTAFATPIDAREGVGSGMSAYD
                     RARTIEVAIADNTRPADLIRPGRLQTLRAVEGGVLARRGHTEAAVDLARLAGLKPAGV
                     ICEIMNEDGTMARMPDLERFAAQHGISILRISDLVAYREMERLIRRRAETVLPTRKAG
                     DFRLLIYSDNLETTLYVALVKGEIRADQPTLVRLHSQCLTGDVFGSERCDCGEQLDAA
                     LGMIDQEGSGVLVYMFEEGRGIGLLNKVRAYALQDQGHDTIEANHALGFAADMRDYKA
                     GAHILFDLGVKQVRLMTNNPEKVAALEDYGLTVTTRVPIEAMPRSANRGYLETKRTRF
                     GHLLSLVGDAPAPDKAKE"
     gene            complement(1329..1979)
                     /locus_tag="VFY80_00870"
     CDS             complement(1329..1979)
                     /locus_tag="VFY80_00870"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX6006480.1"
                     /translation="MLKRNIIPLLALLLCGAALAHAADINVIGLMSGKAVLVIDGGKP
                     RTLSVGQATPEKVRLISATSEEAVVEVGGERRRLTLGDRISMGSGRAGAQSTTISADA
                     RGHFITTAAVNGISLRFLVDTGASVVTLSAADARRAGVSYLKGPRGLLQTANGVVSAY
                     RVKLDTVTLGPITLHNVDGVVIDNDMMGGVALLGLSFLNRTEMRRDGDTMTLTRRY"
     gene            1995..>2245
                     /locus_tag="VFY80_00875"
     CDS             1995..>2245
                     /locus_tag="VFY80_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006481.1"
                     /translation="MKPVAIMRHAPTEGPGYFATCLERHGVPWRIVRIDADEQLPELS
                     ECSGLAFMGGPMSVNDELAWIAPALALIRRAVAEAIPVIG"
BASE COUNT          411 a          734 c          726 g          374 t
ORIGIN      
        1 aatacatcgt cgagcgcgtc ctgcccgacg tccgccgggg gcgcatggtg atcctcgtgg
       61 acagcgagga tcgcgagaac gagggggacc tcttcgtcgc ggcggaacgc gcgactccgg
      121 aagcgatcaa cttcatggcg cagcacgggc gcggtctcat ttcgctcgcg ctcaccgagg
      181 agcggatgcg cgcgctcggc cttgcgctca tcgccgacga ccagatcaac accaccaaat
      241 tccagacggc gtttgcgacg ccgatcgacg cgcgagaggg cgtgggctcc ggcatgtcgg
      301 cctacgaccg ggcccgcacg atagaagtcg cgatcgcgga caacacccga ccggcggatc
      361 tcattcgtcc cggacgcctg cagacgctcc gggcggtgga gggtggcgtg ctcgcgcgtc
      421 gcgggcatac cgaggcggcg gtggaccttg cccgcctcgc aggcttgaag cctgccggcg
      481 tcatctgcga gatcatgaac gaggacggca cgatggcgcg gatgccggac ctcgaacgct
      541 ttgccgcgca gcacggcatc agcatcctgc ggatctccga tctggtcgct tatcgcgaaa
      601 tggaacggct gatccgccgc cgcgccgaga ccgtgctgcc cacgcggaag gcgggtgact
      661 ttcgcctgct catctacagc gacaacctcg agaccacact ttatgtcgcg ctggtgaagg
      721 gcgagatccg tgccgaccag ccgacgctcg tgcggcttca ttcacagtgc ctgacggggg
      781 acgtcttcgg cagcgagcgc tgcgactgcg gagagcagct cgatgccgcc ctcggcatga
      841 tcgaccagga gggcagcggc gtcctcgtct acatgttcga ggaagggcgg ggcatcgggc
      901 tcctcaacaa agtccgcgcc tatgcgctgc aggatcaggg ccacgacacg atcgaagcga
      961 accatgcgct cggcttcgcc gccgacatgc gcgactacaa ggcgggcgcg cacattcttt
     1021 tcgatctcgg ggtgaagcag gtgcggctca tgaccaacaa tcccgaaaaa gtggccgcgc
     1081 tggaagacta cggactgacc gtcactacgc gcgtgccgat cgaagcgatg ccgcgttccg
     1141 ccaaccgcgg ctatctcgag accaagcgca cccgcttcgg gcatttgctg tccctcgtcg
     1201 gcgacgcgcc agctccggac aaagcgaaag agtgagggac ggccttaagg gctgggccgg
     1261 aatgcaatca ggacagccca caaaaagctt gggccacagt tcgtcaaaca agcgggctcg
     1321 agaggcggct agtagcggcg ggtgagggtc atcgtgtcgc cgtcgcgccg catctccgtg
     1381 cgattgagaa agctcagccc gagaagcgcg acacccccca tcatgtcgtt gtcgatcacc
     1441 acgccatcga cgttgtggag cgtaatgggt ccgagcgtga ccgtgtcgag cttcacgcgg
     1501 taggcggaaa cgacgccgtt tgcggtctgc agcaggcccc tgggtccctt gaggtacgag
     1561 acacccgcgc gccgtgcgtc cgcggcgctc agcgtcacca ccgatgcgcc ggtatcgacg
     1621 agaaatctca gggaaatacc gttcacggcg gcagtcgtga tgaagtgtcc gcgcgcatcg
     1681 gcgctgatcg tggtgctctg cgcgccggct cttccgcttc ccatcgaaat gcgatcgccg
     1741 agcgtgagcc tgcgccgctc tccgcccact tcgaccaccg cctcctcgct cgtcgcactg
     1801 atcaacctga ccttctccgg cgtcgcctgg ccgaccgaca gcgtgcgcgg ctttccgccg
     1861 tcgatcacga gcaccgcctt ccccgacatg aggccgatga cgttgatatc ggcagcgtgc
     1921 gccagcgctg cgccacacag taacagggcg agcagtggaa taatatttct tttcagcata
     1981 ccctcatcgc cacgatgaaa ccggttgcca tcatgcgcca cgcgcctacc gaaggcccgg
     2041 gctattttgc cacatgtctg gagcggcacg gcgtgccgtg gcgaatcgtc aggatcgatg
     2101 ccgacgagca gctgcctgag ctttccgaat gcagtgggct cgcgtttatg ggcgggccga
     2161 tgagcgtaaa cgacgaactg gcatggattg cgccggcgct cgcactgatt cgccgggcgg
     2221 tggctgaagc gatacccgtg atcgg
//
LOCUS       DASURG010000055         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_726917, whole genome shotgun sequence.
ACCESSION   DASURG010000055 DASURG010000000
VERSION     DASURG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726917"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFY80_00880"
     CDS             <1..892
                     /locus_tag="VFY80_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6006482.1"
                     /translation="IQGVILTVPNEQHYPLAEEVAKARKHVYTEKPIAQTLEDGLKIE
                     ALEKRHGVTVTVGHSARLLAGVRLIKERIDKGELGRIAFIEANFSNERALELTPNTWR
                     WYKDRAPGGPLSQLAIHQFDILHLLGGEIVEVNSMASKLSPVGAEVDDQSMTLIRFAD
                     GKVGYVGSCWTSPGIFSVRVFGQKALMHYEVDFGTWDTPHLLHRTSSLYIQRGKDGYG
                     KREDIALPEGDMFRAELEMFAESCERGVPNELTAHNGNVAVAVVYAALKSVERKGEAV
                     RLAEVMEESRARIVAQARAA"
     gene            909..1718
                     /locus_tag="VFY80_00885"
     CDS             909..1718
                     /locus_tag="VFY80_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX6006483.1"
                     /translation="MSKLPTRHFIDLTQPEIAAQLKRNPLVILPAGSVEQHGPHLPTG
                     TDIYASNVIGHAVAERMDGLVLPGGPLGVTPMHMPFEGTITLSPETYQRVVIETGVST
                     VKHGAKYLLILNWHEGNIPSLAIAAESLHRDHGMTVLTVQACYVAAELYGHDCGGLTH
                     GGEIEALAVLAYRPDLVHLDRIEYSSDHSHGRKWDKLRRTRSYQPVLRDIRTIAPTGW
                     YGSPEHATVDKGLRMLNDIADAIAKEASEIFAMLDEAQGGTAEIKHLKMAS"
     gene            1730..2707
                     /locus_tag="VFY80_00890"
     CDS             1730..2707
                     /locus_tag="VFY80_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6006484.1"
                     /translation="MPYDVVCLRPEADFRRVNALPPPSFTITYRAPDDAELAALVGEA
                     RALVIPAVGPRLPAELFERSTVRLVQVTGAGVDRLDEPALKRLGIPVCNVPGGSNRAV
                     AEYAAACASTLLRRLAWASAEVRAGRYEPFRKRMLADNLAGIEALKVGIVGLGVIGLA
                     VAEAFHALGARIAYYDPAPRDPLAAAALGAQSLALEALLATSDVVTLHVPLLPATEGL
                     IGERELAAMKPGAILINASRGGVVDEKALAASLDSGRLGGAAVDVYSTEPPAPDNPLL
                     QLSGEAAAKLILTPHIAGVNRQSFAHLFKAAWQNVERVLRGETPVNRVY"
     gene            2720..>3056
                     /locus_tag="VFY80_00895"
     CDS             2720..>3056
                     /locus_tag="VFY80_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6006485.1"
                     /translation="MNEKQLGIAVVGAGRIGTLRARLSAKHPAVGYIAISDRDPARAR
                     ALAEQTGAQFHSADNLAVIERPEVNAVFVSTPEGEHAHPVRRALQLGKPVLVEKPIAL
                     SLADADAMLE"
BASE COUNT          568 a          996 c          997 g          495 t
ORIGIN      
        1 catccagggc gtcatcctca cggtgccgaa cgagcagcat taccccctcg cggaagaagt
       61 ggcgaaggca cgcaagcacg tctataccga aaagccgatt gcgcagacgc tcgaagacgg
      121 gctcaaaatc gaggcgctcg aaaagcgcca cggcgtcacg gtgaccgtcg gccacagcgc
      181 gcggctgctc gccggagtgc gcctcatcaa ggaaagaatc gataagggcg agctcggccg
      241 tattgctttc atcgaagcaa acttctccaa tgagcgcgcg ctcgagctca cgccgaatac
      301 ttggcgctgg tacaaggatc gcgcacccgg cggcccgctg tcgcagctcg cgatccatca
      361 gttcgacatc cttcatctcc tcgggggcga aatcgtcgag gtaaactcga tggcgtccaa
      421 gctctcgccg gtcggcgcgg aagtggacga ccaatcgatg acgctcatcc gcttcgcgga
      481 tggcaaagtc ggatacgtcg gctcgtgctg gacctctccc ggaatcttca gcgtgcgcgt
      541 attcggccag aaggcgctca tgcactacga agtggacttc ggtacgtggg acacgccgca
      601 cctcctgcac aggacatcga gcctctacat ccagcgcggc aaggacggct acggcaagcg
      661 cgaggatatc gcgctgcccg aaggcgacat gttccgcgcg gagctcgaaa tgtttgcgga
      721 gagctgcgag cgtggcgtgc cgaacgagct gacggcgcac aatggcaacg tggcggtggc
      781 cgtggtctat gccgccctga agtcggtcga gcgcaaaggc gaagccgtgc gtctggcaga
      841 ggtcatggaa gaaagccgag cccggatcgt cgcccaggcg cgcgcggctt gatagaggag
      901 agcgcagcat gtcgaaattg ccgacgcgtc acttcatcga tctcacgcag cctgaaatcg
      961 cggcgcaact gaaacgcaat ccgctggtca tcttgccagc gggcagcgtc gaacagcacg
     1021 gcccgcacct gccgaccggc accgatatct acgcctcgaa cgtgatcggt cacgccgtag
     1081 ccgaacgcat ggatgggctg gtgttgccgg gcgggccact cggcgtgacg cccatgcaca
     1141 tgccgttcga gggcaccatc acgctcagtc ccgagaccta ccagcgcgtc gtcatcgaga
     1201 cgggggtctc cacggtgaag cacggcgcga agtatctcct catcttgaac tggcacgaag
     1261 gcaacatccc ttccctcgcc atcgcggcgg aatcgctgca tcgcgaccac ggtatgacgg
     1321 tgctcacagt acaggcctgt tacgtcgctg ccgagctcta cggccatgat tgcgggggcc
     1381 tcacgcacgg gggcgagatc gaagcgctcg ccgtgctcgc ataccggccg gatctcgtgc
     1441 acctggaccg catcgagtac tcgtccgacc actctcacgg ccgcaagtgg gacaagctgc
     1501 gccgtacgcg cagttaccag ccggtgctgc gcgacatccg caccatcgca ccgaccgggt
     1561 ggtacggttc gccggaacat gcgacggtcg acaagggact gcgcatgttg aacgacatcg
     1621 cggacgccat tgcgaaagag gcgagcgaga tctttgcgat gctcgacgag gcgcagggcg
     1681 gaactgccga aatcaagcat ctcaagatgg cgagctgagg cggcgcgcca tgccctacga
     1741 cgtcgtatgc ctgcggcccg aggctgactt caggcgcgta aacgccctgc cgcccccgag
     1801 cttcacgatc acgtatcgcg ctccggatga cgccgagctt gccgcgttgg tcggggaagc
     1861 gcgcgcgctc gtcattccgg cggtgggacc caggctgccg gccgagctct tcgagcgcag
     1921 caccgtgagg ctcgtgcaag tcactggcgc cggtgtcgac cgcctcgatg agcctgcgct
     1981 gaaacgtctc ggcatacccg tgtgcaatgt gccgggcggc agcaaccgcg ccgttgcgga
     2041 atacgccgct gcctgtgcgt cgacgctgct caggcgtctc gcgtgggcga gcgcagaagt
     2101 gcgcgccgga cgctacgagc cctttcgcaa gcgcatgctg gcggacaatc tcgcgggtat
     2161 cgaggccttg aaggtaggca tcgtcggact gggcgtaatc ggtcttgcgg tggcggaggc
     2221 gtttcacgcg ctcggtgcgc ggatcgccta ctacgacccc gccccgcgcg acccgctcgc
     2281 ggcagcagcg ttgggcgcgc aatcacttgc gctcgaggcg ctgctcgcaa cttcggacgt
     2341 ggttacgctc cacgtcccgc tgctccctgc cacagagggc ctgatcggcg agcgcgagct
     2401 ggcggcgatg aaaccggggg cgatcctgat caatgcgtcg cgcggcggcg tggtcgatga
     2461 gaaagcactc gccgcatccc tggattcagg ccggctcggc ggcgctgccg tggatgtgta
     2521 ttcgaccgag ccgcccgcgc cggacaaccc gctgctacag ttgagtggcg aggcggccgc
     2581 aaaattgatc ctcacaccgc acatcgcggg cgtgaaccgt cagtcgttcg cgcatctatt
     2641 caaggctgca tggcagaacg tcgagcgcgt gctgcgcggc gaaacgccgg tcaatcgcgt
     2701 ctactaggag tcgctcccca tgaacgaaaa gcaactcggc atcgccgttg tcggcgccgg
     2761 gcgcatcgga acgttgcgcg cgcggctatc cgcgaagcat ccggcggtcg gctacatcgc
     2821 gatatccgac cgtgatccgg cccgcgcccg agcgctcgca gagcagaccg gcgcgcaatt
     2881 tcattcggcg gacaatcttg ccgtcatcga gcgtccggaa gtgaacgcgg tattcgtctc
     2941 gacgcccgag ggcgagcatg ctcacccggt ccggcgcgcg ctgcagctcg gcaagcccgt
     3001 gctggtggag aagccgatcg cgctttcgct cgcggacgcg gacgcaatgc tcgaaa
//
LOCUS       DASURG010000056         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_114674, whole genome shotgun sequence.
ACCESSION   DASURG010000056 DASURG010000000
VERSION     DASURG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114674"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFY80_00900"
     CDS             <1..309
                     /locus_tag="VFY80_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006486.1"
                     /translation="GMTTAMMVALGENGIKTLEDFADCATDDLVGWTERKKEKDAEPI
                     RHKGVFDGFDIGRKEAEDMIMAARVLLGWIEPPAEEVAEGEEGEAGDEAAAGEAGSSE
                     "
     gene            343..1044
                     /locus_tag="VFY80_00905"
     CDS             343..1044
                     /locus_tag="VFY80_00905"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6006487.1"
                     /translation="MAIRADIAEKPVAERQSPLRTCVVSRAQKPPEELIRFVPGPDGQ
                     IVPDVARRLPGRGVWVTAERGAVAEAVKRKAFARSLRRQVSVQPDLADCVERLLIKRA
                     MHALSLANKAGLVTTGFTRIEAAIINGSVAALLHGVEAAADGVGKLNRRFQAVSAQIG
                     RPAPIIQELTVEQMSLALGRSNVVHAALSAGGATNNFLNEVGRLTRYRSGASASGREQ
                     AADDAAQGTFRTGKV"
     gene            1131..>3126
                     /gene="infB"
                     /locus_tag="VFY80_00910"
     CDS             1131..>3126
                     /gene="infB"
                     /locus_tag="VFY80_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6006488.1"
                     /translation="MQQKFSHGRSKSVVVEKKRKRTLVGQEPEETPAATQARLRPEQP
                     QQRPAAAPAPKPQEQQPQPKILRTLSEEEQQARARALAAAQARELEEQRLREERARQE
                     AERRQREEEERKKAAAEAPTVTEPETAEAAQAAEPAAGAAAQPEEVAPVEANTETREE
                     RPEEPREPAEAAQPTAAEQPQTPEVPQVLPQPVGMTRPIIVSRPERPKPAETAKKAEP
                     EVAPKRALKPGALAELADEEEGRAKKKGGKLLRAPLKGADDTRVRGKLTITNAFDERQ
                     RERSLASLKRRREREKLRAMGIPQSREKIVREVVIPEAITIQELSNRMSERAVDVIKF
                     LMKQGAMHKITDVIDADTAELIVQEFGHTPKRVSEADVEEGFIGADDAPEMLKPRPPV
                     VTIMGHVDHGKTSLLDAIRHANVAAGEAGGITQHIGAYQVKTQSGETITFIDTPGHEA
                     FTAMRARGAKVTDIVVLVVAADDGVMPQTVEAINHARAANVPIIVAINKIDKPDADPS
                     RVRTELLQHEVVVESMSGDVLEVEVSALKRMNLDKLLEAISLQAELLELKANPDRSAE
                     GFVIEAKLERGRGPVGTLLVQRGTLHVGDIVVAGTAWGRVRALINDKGENVKDAGPSV
                     PVEVLGFDSAPEAGDQFAVVENEARAREITDYRIRKRRDTL"
BASE COUNT          639 a          955 c         1090 g          442 t
ORIGIN      
        1 ggcatgacca ccgcgatgat ggtcgctttg ggtgagaacg ggatcaaaac cctggaagac
       61 ttcgcagact gcgccaccga cgacctcgtc ggctggaccg agcggaagaa ggaaaaggat
      121 gccgagccca ttcgccacaa gggcgttttc gacggcttcg atatcggccg gaaagaggcc
      181 gaggatatga tcatggcggc tcgcgtgctg ctgggatgga tcgagcctcc ggccgaagaa
      241 gttgccgagg gtgaggaagg cgaggcgggc gacgaagctg cggccggcga agccggttcg
      301 agcgaatgat ccgccccgtg acgaggagca gacataaagc ggatggcgat tcgggcagat
      361 atcgcagaga agccggtagc ggagcgtcag tctccgctgc ggacctgcgt tgtctcgcgc
      421 gcacagaagc cgcctgagga gctgatccgc tttgttccgg ggcctgatgg ccagatcgtt
      481 cctgacgtag cgcgccgcct gccgggtcgc ggcgtgtggg tgacggccga acgcggcgcc
      541 gttgccgagg ccgtcaagcg caaggcgttt gcgcgcagcc tgagacggca ggtttcggtg
      601 cagccggacc tggcggattg cgtggaacga ctgttgatca agcgcgcaat gcatgcgctc
      661 agcctagcca ataaggccgg gctcgtaacg actggcttca cccggatcga agcggcaatc
      721 ataaatggaa gtgttgccgc attgctgcac ggcgttgagg ccgcagcgga tggcgtgggc
      781 aagctgaatc gccgctttca ggcggtttcg gctcagattg gccggcccgc gccgatcatt
      841 caggaattga ccgtcgagca aatgagtttg gccttggggc ggtcgaatgt ggtacatgct
      901 gcgctgagcg caggcggtgc cacgaacaat ttcctgaacg aggttggccg attgacccga
      961 taccggtcgg gcgcgtcggc atcgggcaga gaacaagcgg cagatgatgc cgcccaaggg
     1021 acgtttagaa cgggcaaggt atgagcgaaa cgaaagagac aaccgacaag accgcgctgg
     1081 caggtacgcg caagccgctg agcctgaagc ggacggtcga gtccggccac gtgcagcaga
     1141 agttcagcca cggccggtcg aagtcggttg tggtcgagaa aaagagaaag cgcacccttg
     1201 tggggcagga gccggaggag acccctgcgg cgacgcaagc gcgcctgcgc ccggagcagc
     1261 ctcaacagcg gcctgccgca gctcccgcgc ccaagccaca ggagcagcag ccgcagccaa
     1321 agattctgcg cactctctcc gaggaagagc agcaggcgag agcgagagcc ctcgcagccg
     1381 cgcaagcgcg tgagctggaa gagcagcgcc tgcgcgaaga gcgcgcgcgc caggaggccg
     1441 agcgtcgcca gcgcgaagag gaagagcgca agaaagctgc ggcagaggcg ccgaccgtaa
     1501 ccgagcccga gacggccgaa gccgctcagg cagctgagcc ggccgcaggg gccgctgccc
     1561 agccggaaga ggtcgccccg gtagaagcca acacggagac tcgtgaagag cgtcccgagg
     1621 agccgcgtga gcctgctgaa gccgctcagc cgacggctgc cgagcagccc caaaccccgg
     1681 aagtgccgca ggtgcttcca cagcccgtcg gcatgacgcg gccgatcatc gtctcgcgtc
     1741 ctgagcggcc caagcccgcc gagacggcca agaaagctga accggaggtg gcgccgaagc
     1801 gcgccctcaa gcccggtgct ctcgccgaac ttgcggatga agaggaaggc cgcgcgaaaa
     1861 agaagggcgg caaacttctt cgtgcgcctc tgaagggcgc cgacgacacc cgcgtgcgtg
     1921 gcaagctgac gatcacgaac gctttcgacg agcgccagcg cgagcggtca ctcgcctcgc
     1981 tgaagcgtcg tcgcgagcgc gagaagctgc gcgccatggg catcccccag tcgcgcgaga
     2041 agatcgtgcg cgaggtggtg atccccgagg cgatcacgat ccaggagctc tcgaaccgta
     2101 tgtcggagcg cgccgtcgac gtcatcaagt tcctgatgaa gcagggcgcg atgcacaaaa
     2161 tcacggacgt gatcgatgcc gacacggccg agctcatcgt gcaggagttc gggcatacgc
     2221 cgaaacgtgt gtccgaggct gacgtggagg aaggcttcat cggcgccgat gatgcgcccg
     2281 agatgctcaa gccgcggccg ccggtggtca ccatcatggg ccacgtcgac cacggcaaaa
     2341 cctcgctgct cgacgcgatc cggcatgcga atgtggccgc cggtgaggct ggcggcatca
     2401 cccagcacat tggtgcgtat caggtgaaaa cgcagtctgg cgaaacgatc accttcatcg
     2461 atacgccagg ccacgaggcg ttcacagcca tgcgcgcccg cggtgccaag gtgaccgaca
     2521 tcgtcgtgct ggtggttgcg gcggacgacg gcgtgatgcc ccagacggtg gaggccatca
     2581 atcacgcccg ggcggccaac gtgccgatca ttgttgccat caacaagatc gataagccgg
     2641 acgcagaccc gagccgtgtc cgaacggaac tgcttcagca cgaagtggtg gtcgaaagca
     2701 tgtcgggtga cgtgctcgag gtcgaagtat cggccctgaa gcgcatgaac ctcgacaagc
     2761 tcctcgaggc gatttcgctc caggccgagc tgctcgaact caaggccaac ccggaccgct
     2821 ccgcggaggg cttcgtcatc gaggccaagt tggagcgtgg tcgtggccct gtgggcacgc
     2881 tgctcgttca gcgcggtacg ctccacgtcg gcgatattgt cgtcgccgga acggcctggg
     2941 gccgcgtgcg cgcgctcatc aacgacaagg gcgagaacgt caaggacgct ggtccctcgg
     3001 ttccggtcga ggtgctgggc ttcgactccg cgcccgaggc cggcgaccag ttcgccgtgg
     3061 tcgagaacga ggcgcgcgcc cgcgagatca cggactaccg catccgcaag cggcgcgaca
     3121 cgctgg
//
LOCUS       DASURG010000057         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_625754, whole genome shotgun sequence.
ACCESSION   DASURG010000057 DASURG010000000
VERSION     DASURG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625754"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /gene="tcuA"
                     /locus_tag="VFY80_00915"
     CDS             complement(<1..1443)
                     /gene="tcuA"
                     /locus_tag="VFY80_00915"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6006489.1"
                     /translation="MNTSIFGTPDVIVVGAGNAAACAALAARESGANVIMLEAAPIDE
                     CGGNSRYTAGLMRVVYNGVDDLAQLVPDLTEEEKKLHDFGAYTADNYFDDMGRITQYR
                     TDPDLCEILISRSFETLVWLRKKGVKFQASYGRQSYKIDDKRFKFWGGLAAETWGGGP
                     GLVDNEHKACAREGITIHYETPAIALLTDDEGRVIGVRAKHKGRNVEIRSKAVILACG
                     GFESNAEMRTRYLGPGWDLAKVRGTRYNTGLGIRMALDVGALSYGHWSGCHAVGWDLN
                     APPFGDLSVGDNFQKHSYPWGIMVNARGERFVDEGADFRNYTYAKYGRVILEQPGMFA
                     WQIFDAKIIPVLRDEYRIKRVTKARADTLEELVKKLEGVNAEACLREIQEYNKAVRTD
                     IPFNMAIKDGRCTAGLRINKSNWANTVDTPPFEAYAVTCGVTFSFGGVKVSTDAEVED
                     TAGKPIPGLYAAGEMVGGIFYHNYPGGTGLT"
     gene            complement(1493..2326)
                     /locus_tag="VFY80_00920"
     CDS             complement(1493..2326)
                     /locus_tag="VFY80_00920"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX6006490.1"
                     /translation="MPDAARATSAPAFALPSRSGKPRRRGITSMIDFGPDTFGWTGGE
                     SGIRNVLEVAADYIDYAKIYAMNALLMPEGSVKRAARLYRDAGVVPFAGGILFEYAWQ
                     RSELDGLLALLARLDLPGLELSENYVTLSEAERDRVIERFQNAGLQVVYEFGRKNPED
                     ALSIDYLAAIVKSVAAHGIHHVTVEQCEMDLLVRDAPDAIRTLAAEPWFDAVVIEADP
                     YRFPQQHVELLRDFGPEANLANITPGQVLRLEGFRRGIGRAVNYSLLSGDPSHAAGSH
                     I"
     gene            complement(2342..2785)
                     /locus_tag="VFY80_00925"
     CDS             complement(2342..2785)
                     /locus_tag="VFY80_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6006491.1"
                     /translation="MKKTLFATPQDAEAAFYDAFTKGDLDAMMEVWADDDDIYCVHPG
                     GARMSGVAEVRESWRKIFAAGQSLRFQLRGQHYVNGMMLSAHSVYEHINVAGETRARG
                     PMIATNIYLRTEHGWRMVAHHASPVPAPATAADPEQRRQTKATLH"
     gene            complement(2806..>3477)
                     /gene="waaF"
                     /locus_tag="VFY80_00930"
     CDS             complement(2806..>3477)
                     /gene="waaF"
                     /locus_tag="VFY80_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX6006492.1"
                     /translation="LNDVRSLNERALPRMVQRYAALALARGDPVSPPPPLALRIDEPA
                     RLATLGRLRLDVNLPAAALCPGAEYGPAKRWPLHHFATLGKDLAARGFDVWIVGSGRE
                     RGLGEEIEKLAEGACRNLCGLTTLGEAIDVLASAALVVSNDSGLMHVAAALGRPLVAL
                     YGSSSPEFTPPHTADAKIVRLGLECSPCFARECPLGHFNCMMQLTPERVCSVLDFDKI
                     RLQPR"
BASE COUNT          591 a         1125 c         1117 g          644 t
ORIGIN      
        1 ggtgaggccg gtaccgccgg gataattatg gtagaagatt ccacccacca tttcgcccgc
       61 agcatagagt cccggaatcg gcttccccgc ggtgtcctcg acctccgcgt cggtgctcac
      121 cttgactccg ccgaagctaa aggtgacccc gcacgtcacc gcataggcct cgaacggcgg
      181 agtgtcgacg gtattcgccc agttcgactt gttgattctg aggcccgcgg tgcagcgccc
      241 gtccttgatc gccatgttga acgggatgtc ggtgcgcacc gccttgttgt actcctggat
      301 ttcgcgcagg cacgcctcgg cgttcacgcc ttcgagcttc tttaccagct cttcgagcgt
      361 atccgcgcgc gccttggtga cgcgcttgat gcggtattcg tcgcgcagta ccgggataat
      421 ctttgcgtcg aagatctgcc atgcgaacat gccaggctgc tcgaggatga cgcggccgta
      481 tttcgcgtag gtgtaattgc gaaagtccgc gccctcgtcc acgaagcgct cgccgcgcgc
      541 gttcaccatg atgccccacg ggtagctgtg cttctggaaa ttgtcgccta ccgaaagatc
      601 accgaaaggc ggcgcgttga ggtcccaccc gaccgcgtga cagcccgacc agtggccgta
      661 ggaaagtgcg ccgacgtcga gcgccatcct gatgccaaga ccggtgttgt atcgggtgcc
      721 gcgcaccttc gcgagatccc agccggggcc gagatagcgc gtgcgcattt cggcattcga
      781 ttcgaatccg ccgcacgcca ggatcactgc cttcgaacgg atctcgacgt tcctgccctt
      841 gtgcttcgca cgcacgccga tgacgcggcc ctcatcgtcc gtcagaagtg cgatggccgg
      901 cgtttcgtaa tggatggtga tgccttcacg cgcacacgcc ttgtgctcgt tatcgacgag
      961 tccgggcccg ccgccccatg tctcggcggc gaggccgccc cagaacttga agcgcttgtc
     1021 gtcgatcttg tacgactggc ggccgtagct cgcctggaac ttgacgccct tctttcgcag
     1081 ccacacgagc gtctcgaagc tccgcgagat caggatctcg cacagatcgg gatcggtgcg
     1141 gtactgcgtg atgcgcccca tgtcgtcgaa gtagttgtct gccgtatacg cgccgaagtc
     1201 gtgcagcttc ttctcttcct cggtgagatc ggggacgagt tgcgcaaggt cgtccacgcc
     1261 gttgtacacc acgcgcatca atcccgcggt gtagcggctg ttgccaccgc attcatcgat
     1321 cggtgccgcc tcgagcatga tgacgttcgc gccgctctcg cgcgcggcga gcgccgcgca
     1381 ggcggccgcg ttgccggcgc ctaccacgat gacgtcggga gtgccaaaga tgctggtgtt
     1441 cattgcgatc ctctatcagg ttgcagaaag ccgcccatac cgggcggcac gatcaaatgt
     1501 ggctgcctgc tgcgtgggaa gggtcgcccg agagcagcga atagttcacc gcccgcccga
     1561 tgccgcgccg gaatccctcg agccgcagga cctgacccgg cgtgatgttg gcgagattcg
     1621 cctcggggcc gaaatcgcgc agaagttcga cgtgctgctg cggaaagcgg taggggtccg
     1681 cttcgatcac gaccgcatcg aaccatggct ccgcggcgag cgttcgaatc gcatccggtg
     1741 catcgcgaac gagcagatcc atttcgcact gctccacggt aacgtgatgg atgccgtgcg
     1801 cggcaaccga cttcacgatc gctgcgagat aatcgatgct caaggcatct tccggattct
     1861 tgcggccgaa ctcgtacacg acctgcaggc ccgcattctg gaagcgctcg attacccggt
     1921 cgcgctctgc ttcggagagc gtcacgtagt tctccgagag ctcgagcccc ggcagatcga
     1981 ggcgtgccag caacgcgaga agcccgtcca gctcgctgcg ctgccacgca tattcgaaaa
     2041 ggatgccgcc ggcgaaaggg acgacgcccg catcgcgata gaggcgcgct gcgcgcttta
     2101 ccgagccttc cggcatgagc aatgcattca tcgcgtagat cttggcgtaa tcaatgtagt
     2161 ccgccgcgac ctcgagcacg ttgcgtatgc cggattcgcc tccggtccag ccgaaagtgt
     2221 cggggccgaa atcgatcatc gatgtgatgc cgcgtcgccg cggcttgccg ctgcgcgacg
     2281 gcagcgcgaa cgccggtgcg cttgtcgctc gtgccgcgtc aggcatggtg cgccatgccc
     2341 gtcaatggag cgtcgccttg gtctggcggc gctgctcggg atcggccgcg gtagccggtg
     2401 caggaaccgg cgaagcgtga tgggcgacca tgcgccagcc gtgctcggtg cgaagataaa
     2461 tgttggttgc gatcatcggg ccccgcgcgc gcgtttcacc cgcgacgttg atgtgctcat
     2521 agacgctgtg cgccgagagc atcatgccgt tcacgtagtg ctgcccgcgc aactgaaagc
     2581 gcagcgactg gccggcagcg aagattttgc gccacgactc gcgcacctcc gcgacaccgc
     2641 tcatgcgcgc gccgcccgga tgcacgcaat agatgtcgtc gtcgtcggcc cagacctcca
     2701 tcatcgcgtc gagatcgccc ttggtaaagg catcgtagaa cgcagcttcg gcgtcctggg
     2761 gcgtggcaaa gagcgtcttc ttcacgggat cggaagtcgg gcgcgtcaac ggggttgaag
     2821 ccggatttta tcaaagtcga ggacgctgca tacccgctcc ggcgtgagtt gcatcatgca
     2881 gttgaagtgg ccgagcgggc actcgcgcgc gaagcacggg ctgcactcga gtcccaggcg
     2941 aacgatcttc gcgtccgccg tgtgcggcgg cgtgaactcc gggctgctcg agccatagag
     3001 cgcgacgagc ggccttccca gcgcggccgc gacgtgcatc agccccgagt cgttgctgac
     3061 caccagcgca gccgaggcaa gcacgtcgat cgcctccccg agcgtcgtca ggccgcacag
     3121 attgcggcac gcgccttccg cgagcttctc gatctcttcg cccagtccgc gctcgcgccc
     3181 ggagcccacg atccacacgt cgaaacccct tgcagcgagg tcctttccga gggttgcaaa
     3241 gtggtggaga ggccagcgct tggcggggcc gtattcggcg ccggggcaga gtgcggcggc
     3301 gggaagattc acatccaggc gcagtctccc gagcgtcgca aggcgtgcgg gttcgtcgat
     3361 gcgcagcgcg agcggcggtg gaggggagac gggatcgcca cgggcaaggg cgagcgccgc
     3421 gtagcgctgc accatgcgag gcagcgccct ctcgttgagc gagcgaacgt cgttgag
//
LOCUS       DASURG010000058         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_932223, whole genome shotgun sequence.
ACCESSION   DASURG010000058 DASURG010000000
VERSION     DASURG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932223"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFY80_00935"
     CDS             complement(<1..504)
                     /locus_tag="VFY80_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6006493.1"
                     /translation="MKICFVGLDNLPVLAPEFQHLPVGGESVQQALLADALVRRGIEV
                     STVVADWGQEDGRQWQGIRVFKAYRRQAGIPMLRFFHPRWTRLWSALARADADVYYTS
                     CAGMHVGLIAMFCRRRGRRFVFRAASDPDCDRSRLPLLVRYARDRWLYEYGLRRADAI
                     LVQSAGQA"
     gene            complement(524..1672)
                     /locus_tag="VFY80_00940"
     CDS             complement(524..1672)
                     /locus_tag="VFY80_00940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6006494.1"
                     /translation="MNSVRPLRAEQMRPPARAGSLVREARAEDLPHVADLHARVFGST
                     SGRTPRALAEYFEEILFRNPWRDAALPSFVSVRNGEITGFLGVVPRPMRIGQRIIRVA
                     VCTQFMVDPRHRSELAAVQLLKAFFSGPQELSLADGANESARRMWLALGGDAPLLYSL
                     HWVRPLRPARYVLSLLERRTPTLRALAIAGRPVAALADFVAGRIPLNALSGKEAAGWS
                     EEPLRPGTVYAYLPEAMAGTTLQPLYEARTLEWLLDQAARKHRHGSLRARAVRDREQR
                     LAGWFIHYARGGGISEVVQIGARAGAFVPVLQRLVLDAWRVGATALRGRLDPRYAQAL
                     SEQHCWMRRDCNWTLVHSRDKDILCAIHEGKVFLSRLEGEWWMRFVDE"
     gene            complement(1669..1953)
                     /locus_tag="VFY80_00945"
     CDS             complement(1669..1953)
                     /locus_tag="VFY80_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6006495.1"
                     /translation="MRIDADLPDLALYDEIITIFLERLNVTVPSLDADLLASGVLDSL
                     ALVELLVQIEARYGIQISLEKLDFDDFRSVSAIAAFVARERNGAKVQSLQ"
     gene            complement(1943..2896)
                     /locus_tag="VFY80_00950"
     CDS             complement(1943..2896)
                     /locus_tag="VFY80_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX6006496.1"
                     /translation="MSEWRRRKGVNVVLVAEEAAGIQTLRAILKTRHRIVGVVASSQA
                     DAPQRGATVRGVAEANGCRIWPGTAVRDPHFGTILREASVDVLLNVHSLRVIHPAVLE
                     APGIGSFNLHPGPLPEYAGLNAPSWAIYNGEPLHAVTVHWMTAEIDTGPIAFRQEIPT
                     DDADTGLTLSAKCVRAGVPLLVKLLDVAEEDPAAIPSQPQDGSRRRYYGREVPDDGRL
                     HWSQPADRIVRFVRACDYSPLPSPWGHPRTVIRGQPCEIVKAFRTRRACAAPAGTVGP
                     VENDAAFVAAADEWVAISRLSLSGRALRAAEVLKTGACLED"
     gene            complement(2889..>4092)
                     /locus_tag="VFY80_00955"
     CDS             complement(2889..>4092)
                     /locus_tag="VFY80_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6006497.1"
                     /translation="APPETGRPAFTARDLPALPATRPACRGCDTDAAHILFTSGSTGV
                     PKGVTITHRNVAHFIRWAQRYFGTAADDRISQQPPFHFDLSTFDIYCTLWSGAELHLV
                     PAELSLLPHKLVQFMREGELTQWFSVPSVLNLMAKYDAVAAGSLPALKRVLWCGEAMP
                     TPTLIYLMQRLPRARFTNLYGPTEATIASSYYTVARCPQHAREAIPIGSACEGETLLV
                     LDERLRPVAPGEIGDLYIGGVGLSPGYWRDPEKTAAAFIMHRDEDNVERRIYRTGDLA
                     RVGEDGLLYYLGRSDSQIKSRGYRIELGEIEAAAHEIGCLRECAVVAIKSQGFEGHAI
                     CLAYVPQAGESISPAMLRERLKSLLPQYMLPTHWRAFDALPKNPNGKIDRPALRVAFL
                     RREECAHV"
BASE COUNT          751 a         1324 c         1350 g          667 t
ORIGIN      
        1 cgcttgtccg gcactctgga ccagtatggc atccgcacgc cgcaagccgt actcgtagag
       61 ccaccggtcg cgcgcgtagc ggacgagaag cggcaggcgg gagcggtcgc agtcgggatc
      121 gctggcggcg cggaagacga atctgcggcc gcggcgtcgg cagaacatcg cgatcaagcc
      181 gacatgcatc ccggcgcagc tcgtgtagta aacatcggca tcggcacggg cgagcgcgga
      241 ccacagacgt gtccatcggg ggtggaagaa acgcagcatc gggatgccgg cctggcggcg
      301 gtacgctttg aacacgcgga taccttgcca ctgccttccg tcctcctgtc cccagtcggc
      361 cacgactgta gacacttcga tgccacgccg caccagcgcg tcggcaagca gtgcctgctg
      421 cactgattcg ccgccgacgg gaaggtgctg aaactccggt gcgagcacgg ggagattatc
      481 gaggccgacg aagcagatct tcatagcgct caacgtgtgg cccttattcg tcgacgaaac
      541 gcatccacca ttcgccctcc agccggctca gaaatacttt cccttcatgg attgcgcaga
      601 ggatgtcctt gtcgcgcgaa tgcacgagtg tccagttgca gtcgcgccgc atccagcagt
      661 gctgctcgga caacgcctgc gcgtaacgcg ggtcgagcct gccgcgaaga gcggtcgcgc
      721 ccactcgcca cgcgtcgagt acgagcctct gcagcaccgg cacgaaggcg cccgcgcgcg
      781 ctccgatctg cacgacctcg ctgatgccac cgcctcgcgc gtagtgtatg aaccatcccg
      841 cgagccgctg ctcccgatcg cgcacggcac gcgcccgcag gctgccgtgc cggtgcttgc
      901 gcgcggcctg gtcgagcagc cattcgagcg tgcgagcttc atagagcggc tgcaaagtgg
      961 tgcctgccat cgcttcgggg agatacgcgt ataccgtgcc gggacgaagc ggctcctccg
     1021 accagccggc agcctctttt cccgacagcg cattgagcgg gatacggccg gctacgaagt
     1081 cggccagcgc ggcgaccggc cgccccgcga tcgcgagcgc gcggagcgtt ggcgtgcgcc
     1141 gctcgagcag cgagagcacg tagcgcgcgg gacgcaaggg ccggacccaa tgaaggctgt
     1201 agagcagcgg cgcatcgcct cctagagcga gccacatgcg gcgcgcgctt tcgttcgctc
     1261 catccgccag tgaaagctcc tgtggaccgg aaaagaaagc cttcaggagc tggacggccg
     1321 cgagctcgct gcgatgccgc ggatccacca tgaactgcgt gcagacagcg acacggatga
     1381 tgcgctgacc gatccgcatc ggacggggca cgacacccag aaatccggtg atttcgccgt
     1441 tgcgaaccga tacgaaagaa ggaagggcgg catcgcgcca cgggttgcga aaaaggatct
     1501 cctcgaaata ttcggcgagc gcgcggggcg tgcgtccgga agtcgacccg aagacgcgtg
     1561 cgtgcaggtc ggccacgtgt ggcaggtcct cggcgcgcgc ttcgcgcact aagcttccgg
     1621 cgcgtgccgg cgggcgcatc tgctcggctc gtagcgggcg tacggagttc attgcagcga
     1681 ctgcaccttc gcgccgttgc gttcccgtgc aacgaacgcc gcaatggcgg agaccgatct
     1741 gaagtcgtcg aagtcgagct tctcgagcga gatctggatg ccgtatcgcg cctcgatctg
     1801 cacgagcagc tccaccagtg cgagcgagtc gagcacgccg ctcgcaagaa ggtcggcgtc
     1861 gagtgagggg accgtcacgt tgaggcgctc gagaaatatc gtgatgattt cgtcgtagag
     1921 cgcaagatcg ggcaggtcag cgtcaatcct caaggcaggc tcctgttttc aacacttcgg
     1981 cggcgcgcaa cgcacggccg gaaagactga gccggctgat cgccacccat tcatccgcgg
     2041 ccgcgacgaa ggccgcatcg ttttccaccg gaccgaccgt gccggcgggg gccgcgcagg
     2101 cgcgccgggt acgaaacgct ttgacgatct cgcacggctg gccacgaatg acggtccggg
     2161 gatgccccca cggagaaggc agcggagaat aatcgcaggc gcgcacgaac cgcacgatgc
     2221 ggtccgcggg ttgcgaccaa tgcaaacggc catcatctgg aacttccctg ccgtagtaac
     2281 gccggcgaga cccgtcctgc ggttgtgacg ggatagcggc gggatcttcc tcagcgacat
     2341 cgagcagctt cacgagcaac ggcacgcccg cgcgcacaca cttggcgctc agcgtgagcc
     2401 cggtatccgc gtcgtccgtc ggaatctcct gccggaatgc gatggggccg gtgtcgatct
     2461 ccgcagtcat ccagtggacc gtcacagcgt gtagcggttc gccgttgtag atggcccaac
     2521 tcggcgcgtt gagtcctgca tactcgggca gcggccccgg atggaggttg aagctgccga
     2581 ttccgggcgc ctccaggact gccggatgga tcacgcgcag ggaatgcacg ttgagcagca
     2641 catccacgga cgcttcgcgc aggatcgtcc cgaaatgcgg gtcgcgcacc gcagtgccgg
     2701 gccaaatccg gcatccgttc gcctccgcga caccgcggac ggtcgccccg cgctgggggg
     2761 cgtcagcttg cgatgaggcc actacgccga caatgcgatg gcgtgttttc agtatggccc
     2821 gcaaggtctg tatgccggcc gcctcttccg ccacgagcac gacgttcacg cctttgcggc
     2881 gccgccattc agacatgtgc gcattcctcg cgacgcagaa acgctacgcg gagcgccggc
     2941 cggtcgatct tcccgttcgg gttcttggga agcgcgtcga acgcccgcca gtgggtgggc
     3001 agcatgtact gcggcaacag cgacttcagc cgttcgcgca gcatcgcggg agaaatggac
     3061 tctccggcct gcgggacata ggcgagacaa atcgcgtgac cttcgaaccc ttggctcttg
     3121 atcgcaacga ccgcgcattc cctgagacag ccgatctcat gggcagccgc ttctatttcg
     3181 cccagctcga tgcgatagcc ccggctcttg atctgggaat cggaacgtcc caggtaatag
     3241 agcagtccat cctcgccgac acgcgcgaga tcgccggtgc ggtagatacg ccgttcgacg
     3301 ttgtcttcat cccgatgcat gatgaaggcc gcggcggttt tttccggatc gcgccagtag
     3361 ccggggctca ggccgacacc gccgatgtag agatctccga tctcgccggg ggccacgggc
     3421 cggaggcgct cgtctagcac gaggagcgtt tcaccctcgc acgcgctacc aatgggaatt
     3481 gcctcgcgcg catgctgcgg gcagcgcgcc accgtgtagt aactgctcgc gatggtggct
     3541 tccgttgggc catacagatt ggtgaatcgg gcacgcggaa gccgctgcat caggtagatc
     3601 agcgtgggcg tcggcatcgc ttctccgcac cacagcacgc gcttcagcgc gggcagtgag
     3661 cccgcggcaa ccgcgtcgta cttcgccatg aggttgagta ccgacggcac ggaaaaccac
     3721 tgcgtcagct cgccttcgcg catgaactgc acgagcttgt gcgggagcag gcttaactcc
     3781 gccggcacga gatgcagctc ggcgccggac cagagcgtgc aatagatgtc gaaggtcgac
     3841 aggtcgaagt gaaacggcgg ctgctgcgaa atccggtcgt cggctgcagt accgaagtag
     3901 cgctgtgccc accgtatgaa gtgcgcgacg ttacgatgcg tgatcgtgac gcccttcgga
     3961 acgccggtcg agccggaagt gaaaaggata tgtgccgcgt ccgtatcgca cccgcgacac
     4021 gcggggcgcg ttgccggtaa agcgggcaga tcgcgtgcgg tgaacgcggg cctacccgtt
     4081 tccggaggcg ca
//
LOCUS       DASURG010000059         7119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1341177, whole genome shotgun sequence.
ACCESSION   DASURG010000059 DASURG010000000
VERSION     DASURG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341177"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFY80_00960"
     CDS             complement(<1..913)
                     /locus_tag="VFY80_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6006498.1"
                     /translation="MADPTLGAYNIADLRAMALKRVPKGLFEFVDRGTEDEVSLRNNR
                     AVFERIRFKPRTLVDVSKRSQQITLFGKPHKMPIVIAPTGTAGLMWHEGEIALARAAA
                     EAGIPFTLATGSMTAMEKVAERAGGELWFQLYMWPDRRLSHQLVERARAAGFKALVVT
                     VDGVVAGNREYNLRNGFTIPFSFTRRNVTDVLMHPRWLAGVLARYVMTTGMPRYENYP
                     SEIKRRITAQPMGRSQMRNDTLNWNDLRELRKMWPHTLIVKGILHPQDAILAADCGAD
                     AVAVSNHGGRNLDGAISPIEALPEVVDA"
     gene            complement(906..1121)
                     /locus_tag="VFY80_00965"
     CDS             complement(906..1121)
                     /locus_tag="VFY80_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006499.1"
                     /translation="MMQFKRIRGQVLVPKAWRGLAVREKTALVRELSQVAELGCDAMA
                     HGARRCPISPLLYNPTAGAVIREVRHG"
     gene            complement(1118..1435)
                     /locus_tag="VFY80_00970"
     CDS             complement(1118..1435)
                     /locus_tag="VFY80_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HEX6006500.1"
                     /translation="MSYANRLAHIEGRKARLIAQCARQRDQFAAELNAWRGPVSVIDR
                     GIQAGRLLKAHPVIAAVLLIAVAVIGRRHLLRWAGRGLIVWKFWNTVRSALPARGAWS
                     REA"
     gene            complement(1432..1830)
                     /locus_tag="VFY80_00975"
     CDS             complement(1432..1830)
                     /locus_tag="VFY80_00975"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX6006501.1"
                     /translation="MASDPSTRGGEPPAGLMQSLRNLAATGVGMLQTRLELLLSEIEE
                     ERVRLLQLLVWGFAALLFLAFALLMFTLAVVAAFWDTHRLLALLALGILYLAIGLALA
                     FGARRRAERPKMFASSMAELRKDHETLGSS"
     gene            complement(1843..2154)
                     /locus_tag="VFY80_00980"
     CDS             complement(1843..2154)
                     /locus_tag="VFY80_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX6006502.1"
                     /translation="MTSENPASSEKLMQDLRVVVADAEELLRATASQAGERAAAARAK
                     IEQSLERARAKLTEVETALAERSRQVAHATDEYVHEHPWTAVSVAAGIGLIIGLLISR
                     R"
     gene            2321..3850
                     /locus_tag="VFY80_00985"
     CDS             2321..3850
                     /locus_tag="VFY80_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006503.1"
                     /translation="MRPSHLKASSLAMAVASAACPPLAAGADTPNGTAITIYSTLRPG
                     AVPPELYRDAGRGQSVPGYAMVRNQRELALKTGRNTVRFTDVAALIDPTTVSFESLTD
                     SKGTTVIEQNFQFDLVNSQRLLQKYVDRAITVDQVRGDAVESFQGTLLSVAGGLTLKR
                     DNGTIHILPHNAGIRLPELPGGLITRPTLVWDIAAKTAATHRTRVSYQTGGITWWSDY
                     NVTYEDGAHANACKLDIGAWVSIVNQSGASYADAKLKLVAGDVQRIAPSGRVHREPTA
                     ARAMAMEDRAPGFEEKAFFEYHLYTLGRATTIPDNSTKQIELFSAARGVPCEKTLVYY
                     GLTPGHGFLPNPITDRNYGTQSNKKVDVYLGFKNAKENNMGMPLPAGRVRVNKLDTTD
                     STLEFIGEDKIDHTPRNEKVLIKMGSAFDVVGERRQVAFSVDTSRKTMSEEIEVKLRN
                     HKKAAVTVIVKENLYRWVNWEIVTKSHDYKKQDARTVHFPLRVAADGETTLRYTVQYT
                     W"
     gene            complement(3862..4347)
                     /locus_tag="VFY80_00990"
     CDS             complement(3862..4347)
                     /locus_tag="VFY80_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006504.1"
                     /translation="MNDLMQRGYAYELVEPTGRNFHPDFRPELTPKEMLRLGVFGGKY
                     MTDCAAEYPSDWFERAKLCVERHDPQLNCFGVNASQPLAVWRAKGWIYHEDPRGWFQW
                     YCRYYLGRRCADDERQIRRWRAIARHVAQIRRNCQRGDLGCRPRQRQAVLHWAYDARK
                     I"
     gene            complement(4373..5566)
                     /locus_tag="VFY80_00995"
     CDS             complement(4373..5566)
                     /locus_tag="VFY80_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6006505.1"
                     /translation="MTPSELPEGELAFSYFSDDYRWSHGVLIALGGAPWGGGEIGEIH
                     RIGLRLKNHVGDDRAWFREWCAEAERLEAIAEERAAKGHKLGAASYLLRAAHYYHVGE
                     RFVQPKHDESQAAYRRGVEAFRKAAGFIRRPRIEHVEVPYEGKSLPGMIVHADRAPGR
                     PAQGPVMLFLDGFDVTKEIQYFRGIPDLAARGIACLILDGPGNGESIRFRGLTLHHET
                     ERHAGAAYDYLAARPEFDPKRIGIMALSLGGYYAPRAAAMDGRFACCISWGPEWDYHT
                     KWKQRLERIERGEVLSLSVPWEHLLWVFGVKTKEEAFRKLEGFKLDGVIQRMRCPYLL
                     IHGEGDQQAPFEHAQKMIAAAGSARKELKVFTREEGGYHHCQVDNVSIGTCYMWDWLE
                     DVLQP"
     gene            complement(5597..6553)
                     /locus_tag="VFY80_01000"
     CDS             complement(5597..6553)
                     /locus_tag="VFY80_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006506.1"
                     /translation="MTLSSRLLALACLAALAGVCHAQTQRPVRVLVASAPGGPSDVQA
                     RLITPRMSEALTRTLVVDNRPSANGLLATELCARAEPDGATICVGNSGTHAVNATLYR
                     KLAYDPVRDFIPISQFSTTGMVLAANPKLPGRTVQDLAAHAKANPGRTNLAIAGATGQ
                     LAGDALWARLGIQMNNVPYKGSSPSEMAVVAGESQLSLLTPLASAAHIKSGRMKAYGI
                     TSPKRHYLLPDVPTVAEQGVTGYDFQFWNGMFAPAKTSREQVLRFHKAIRTALDTPEV
                     KERFTQLGLVIVGSTPEEFAKVVKTDVEKFRKIILESGIPRL"
     gene            6733..>7119
                     /locus_tag="VFY80_01005"
     CDS             6733..>7119
                     /locus_tag="VFY80_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006507.1"
                     /translation="MKQILLYGLALAVAGALASGPSYADKPAWAGGDRQEKGKGHSKE
                     RDRGGKRNDDRGGGPRAELHFSDQHRVVIRDYYATEFRSGHCPPGLAKKRNGCMPPGQ
                     AKKWRTGHPLPRDVIYYDLPPALVVKI"
BASE COUNT         1330 a         2277 c         2238 g         1274 t
ORIGIN      
        1 ccgcatcgac cacctcgggc agcgcctcga tcggtgagat ggcaccatcg aggttgcgcc
       61 cgccgtggtt ggagacagcc accgcgtcgg cgccacagtc cgccgcgagg atcgcatcct
      121 ggggatggag gatgcctttc acgatcagtg tgtgcggcca catcttgcgc aactcgcgca
      181 gatcgttcca gttgagcgta tcgttgcgca tctgtgagcg ccccatgggc tgcgccgtga
      241 tgcggcgctt gatctcactc ggatagtttt catagcgtgg catcccggtt gtcatgacgt
      301 agcgcgcaag cacgcctgcg agccagcgcg gatgcatgag aacgtcggtc acgttgcggc
      361 gggtgaagct gaagggaatg gtgaagccgt tgcgcaggtt gtattcccgg ttaccggcga
      421 cgacgccatc gactgtcacc acgagcgcct tgaatcccgc ggcgcgcgca cgctcgacga
      481 gctgatgcga gagcctccga tcgggccaca tgtaaagctg aaaccacagc tcgcctccgg
      541 cgcgctccgc gaccttttcc atcgccgtca tcgagcccgt cgcgagcgtg aacgggatgc
      601 cggcctcggc ggcggcgcgc gcgagcgcga tctcgccctc gtgccacatg agaccggcgg
      661 taccggtggg cgcaatgacg atcggcatct tgtggggctt gccgaagagc gtgatttgct
      721 ggctgcgctt cgacacatcg acgagtgtgc gaggcttgaa ccggatgcgc tcgaagaccg
      781 cgcggttgtt ccgaagcgac acttcgtcct cggtgccgcg atcgacgaac tcgaagagac
      841 ccttcggcac gcgtttgagc gccatcgcgc gcagatcggc gatgttgtat gcgcccagag
      901 tcgggtcagc catgacgaac ctctcggatg acggcgcctg ctgtcggatt gtacagcagc
      961 gggctaatgg ggcatcgccg cgcgccgtgg gccattgcgt cgcagccgag ctccgcgacc
     1021 tgcgagagct cgcgcacgag cgcagtcttc tcgcgcaccg ccagtccgcg ccaggccttc
     1081 ggcacgagca cctggccgcg gatgcgcttg aattgcatca tgcctctctg ctccatgccc
     1141 cgcgcgcagg cagagcgctg cgcacggtgt tccagaactt ccatacgatg agtccgcggc
     1201 cggcccaacg gaggagatgg cggcgtccaa tcacggcgac ggcaatgagc aggactgcgg
     1261 cgatcacggg atgcgccttg agcagacggc cggcctgtat gccgcggtcg ataactgaaa
     1321 caggaccccg ccacgcgttc aattcggcgg cgaactgatc gcgctgacgg gcgcactgcg
     1381 cgatgagccg cgctttgcgc ccctcgatgt gcgcgagtcg attggcgtag ctcaagacga
     1441 accgagtgtc tcgtgatctt tccgaagctc cgccatgctc gaagcgaaca tcttcggacg
     1501 ctcggcccgc cggcgagcgc cgaaagccaa agcgaggccg attgcaagat aaagaattcc
     1561 cagtgcgagc agagcaagca ggcggtgcgt atcccagaag gccgcgacga cggcgagcgt
     1621 gaacatgagc agcgcgaaag cgaggaagag cagcgcggcg aagccccaca ccaggagctg
     1681 caggaggcgt acgcgctcct cttcgatttc gctcagcagc agctcgagcc gcgtctgaag
     1741 catgccgacc ccggtcgctg cgagattgcg cagcgactgc atgagtcccg ccggcggctc
     1801 tccgccgcgc gtggaggggt cgctcgccat aaagacgcgg tgtcagcggc ggctgatcag
     1861 taaaccgatg atcagtccga ttccggccgc aacgctgact gcagtccaag ggtgctcgtg
     1921 gacgtattca tccgtcgcat gcgccacctg acggcttcgc tccgcaaggg ccgtctccac
     1981 ttctgtgagc ttggctcgcg cgcgctcgag gctctgctcg atcttcgcgc gtgccgcggc
     2041 tgcgcgctcg cccgcctggc tggcggtcgc gcgcagcaac tcctctgcgt cagcgacgac
     2101 gactcgcaga tcctgcatga gtttttcact acttgccggg ttttccgatg tcatgacatc
     2161 cctccagggt tcatccgcat ggaaagcgat ggaataggcg gaatttgcag gcaaattata
     2221 ccgttttcgc gcgctttaac cccacatacc gccgcggcac gtttatggtc gtcagaaacc
     2281 ggtcccgcag ccggtatgtg gccaacgaaa ggagaacaaa atgcgtccgt cacatctcaa
     2341 agcctcgtct ctcgccatgg cggtggcgtc cgctgcctgc cccccgcttg cggccggtgc
     2401 ggacactcca aacggcactg cgatcacgat ctacagcacg ctcaggccgg gtgcggtgcc
     2461 gcccgagctc tatcgcgacg ccggccgcgg gcagagtgtt cccggctatg ccatggtgcg
     2521 caaccagcgc gagctggcac tgaagaccgg ccggaacacg gtgcgcttca ccgatgttgc
     2581 cgcgttgatc gatcccacca cagtaagctt cgaatcgctg accgacagca agggcaccac
     2641 ggtcatcgag cagaacttcc agttcgatct cgtgaattcg cagcgcctgt tgcagaaata
     2701 tgtcgatcgc gccatcacgg tggatcaagt gcgcggcgac gcggtcgaat cgttccaggg
     2761 cacactgctt tcggtcgcgg gcggactcac gctcaagcgc gacaatggaa cgatacacat
     2821 ccttccgcac aatgcgggaa tccggcttcc ggagctgccg ggcggcctca tcacgcgccc
     2881 gacgctcgta tgggacatcg ccgcgaagac ggccgcgacg caccgtactc gggtttcgta
     2941 ccagaccggg gggatcacgt ggtggtccga ttacaatgtg acctacgaag acggcgcgca
     3001 tgccaatgcc tgcaagctcg acatcggggc gtgggtcagc atcgtgaatc agtccggcgc
     3061 aagttatgcc gatgcgaagc tgaagctcgt ggcgggcgac gtgcagcgca tcgctccttc
     3121 cggaagagtg catcgtgagc ccacggctgc acgcgccatg gccatggaag accgcgcgcc
     3181 cggttttgag gaaaaagcct tcttcgagta ccacctctac acgctggggc gcgccacgac
     3241 catcccggac aattcgacca agcagatcga gcttttttcc gccgcgcgcg gcgtcccgtg
     3301 cgaaaagacg ctggtctact atgggctcac gcctgggcac ggattcctcc ccaatccgat
     3361 aacggaccgc aattacggca cgcagagcaa caagaaggtg gacgtctacc tcggcttcaa
     3421 gaacgccaag gaaaacaaca tgggcatgcc gcttccggcg ggtcgggtgc gcgtgaacaa
     3481 gctcgacacc acggattcga cgctcgaatt catcggcgag gacaagatcg atcacacccc
     3541 gcgcaacgag aaggtgctga tcaagatggg ctcggcgttc gacgtggtgg gcgagcggcg
     3601 gcaggtcgct ttctcggtgg acacgtcacg caagacgatg agcgaggaaa tcgaagtgaa
     3661 gctgcgcaat cacaagaagg cggccgtgac cgtcattgtc aaggagaacc tctaccgctg
     3721 ggtgaactgg gagatcgtca cgaagagcca cgactacaag aagcaggacg cgcgcaccgt
     3781 gcactttccg ctcagggtcg ccgcggacgg cgagacgaca ctgcgctaca ccgtgcagta
     3841 cacctggtga gcagcgatgc ttcagatctt tcgcgcgtcg taggcccagt gcagtacggc
     3901 ctgtcgctgc ctgggacggc agccgagatc tccacgctgg cagttccgtc ggatctgcgc
     3961 cacgtgccgt gcgatcgcgc gccagcgccg gatctggcgt tcgtcatcgg cgcagcgccg
     4021 gccgaggtaa taacggcaat accactggaa ccagccgcgc gggtcctcgt gatagatcca
     4081 gcccttcgcg cgccagactg caagcggctg cgaggcgtta acgccgaaac agttgagctg
     4141 cgggtcgtgc cgctccacgc acaattttgc cctttcaaac cagtcggacg gatactcggc
     4201 ggcgcagtcg gtcatgtact tgccgccaaa gacgccgagc cggagcatct ccttgggcgt
     4261 cagctcggga cgaaaatcgg gatggaaatt gcgtccggtc ggctcgacga gctcgtaggc
     4321 gtaacctcgc tgcatcaggt cgttcactgc gatccgccgc ggcttcatct tttcagggct
     4381 gcagcacgtc ttccaaccag tcccacatgt agcaggtgcc gatgctcaca ttgtccacct
     4441 gacaatggtg atagccgcct tcctcgcgcg tgaagacctt gagctccttg cgcgccgagc
     4501 cggctgccgc aatcattttc tgcgcgtgct cgaaaggcgc ctgctggtcg ccttcgccgt
     4561 gaatcagcag atacgggcag cgcatcctct ggatcacgcc gtccagcttg aagccttcga
     4621 gtttgcggaa ggcttcctct ttggtcttca cgccgaacac ccagaggaga tgctcccagg
     4681 gtacggatag cgagagcact tcaccgcgct cgatacgctc gagccgctgc ttccatttgg
     4741 tgtgatagtc ccattcgggt ccccatgaaa tgcagcacgc gaaccgtccg tccatcgctg
     4801 cggcgcgcgg cgcataatag ccgccgaggc tcaacgccat gatgccgatg cgcttcggat
     4861 cgaattccgg ccgtgcagcg agatagtcgt acgcagcgcc cgcgtggcgc tcggtctcat
     4921 ggtggagcgt caacccgcga aagcggatcg actcaccgtt ccccggcccg tcgagtatga
     4981 ggcacgcaat gccgcgcgcc gcaagatcgg ggatgccgcg aaagtattgg atctctttgg
     5041 tgacgtcgaa tccgtcgagg aagagcatga cgggcccttg ggcagggcga cccggcgccc
     5101 ggtccgcgtg cacgatcata ccggggagac tctttccttc gtacggcact tcgacgtgct
     5161 cgatgcgcgg tcggcggata aagcccgctg cctttctgaa cgcttcgacg ccgcgccgat
     5221 aggcggcctg gctttcgtcg tgcttcggct gcacgaaccg ttcgccgacg tggtaatagt
     5281 gcgctgcccg caaaagatag gatgccgcac cgagtttgtg gcccttcgcg gcccgctcct
     5341 cagcgatcgc ttccaatcgc tcggcttccg cgcaccattc ccggaaccac gcgcggtcat
     5401 caccaacatg gttcttgagt cgaagaccga tgcggtggat ttcgccgatt tcgccgccgc
     5461 cccatggcgc cccgcccagc gcgatgagca cgccgtgcga ccaccggtag tcgtctgaaa
     5521 aatacgagaa ggcgagttcg ccctccggca gttctgatgg ggtcatgccg tttccattct
     5581 tgatttatac cgcgggctac aggcgcggga tgccggattc gagtatgatc ttcctgaatt
     5641 tctcgacgtc agtcttcacc accttggcga actcctccgg cgtgctgccg acgatgacaa
     5701 gaccgagctg ggtgaagcgc tccttcactt cgggcgtatc gagcgccgtg cggatcgcct
     5761 tgtgaaagcg cagcacctgc tcccggctcg tcttcgccgg tgcaaacatc ccgttccaga
     5821 actgaaaatc atagcccgtc acgccctgct cggcgacggt aggcacatcg ggcagcagat
     5881 aatggcgctt gggactcgtg atgccgtacg ccttcatgcg ccccgacttg atgtgcgctg
     5941 cgctcgcgag cggcgtgagg agcgaaagtt gcgattcacc cgccaccacg gccatttccg
     6001 aaggcgaact gcctttgtag ggcacgttgt tcatctggat ccccagccgt gcccacagcg
     6061 cgtcccccgc cagctgcccc gtcgctccgg cgatggcaag attggtccga cccggattcg
     6121 ccttcgcgtg cgcggcgaga tcctgcaccg tgcggccggg aagcttggga ttcgccgcga
     6181 gcaccatgcc ggtggtggag aactggctga tcggaatgaa atcgcggacg ggatcgtagg
     6241 caagcttgcg ataaagcgtc gcgttcaccg cgtgcgtgcc gctgttgccg acgcagatgg
     6301 tggcaccgtc gggctccgcc ctggcgcaca gctcggttgc aaggagaccg ttcgcgctcg
     6361 gacggttgtc caccaccagc gttcgggtga gcgcctcgct cattctcggg gtgatgaggc
     6421 gcgcctggac gtcggaaggc cctccgggtg cggacgcaac gagcacgcgc accggtcgct
     6481 gggtctgcgc atggcagacc cctgccagcg cagcgagaca tgcgagagcg agcagccgcg
     6541 aggatagcgt catatggacc tcccctgtat ttcacgaaat gaagcgatga tagcggtttt
     6601 cacccgtgaa gtattacgca accgcacccc caggtccgat ttacgctgtg acatatgccg
     6661 cgcttctcgc tggcataacc cgcgcccttg tgcaaagata aaccacactg tgtatcagga
     6721 cgaggagaca tcatgaagca aatcctgttg tacggactcg cgctcgcggt tgcgggtgcg
     6781 ctcgcgagcg gtccttcata tgccgacaaa ccggcatggg cgggcggcga ccggcaggag
     6841 aagggcaaag gccattcgaa ggagcgggac cggggcggca agcgcaacga tgatcgcggg
     6901 ggcggcccgc gcgcggagct ccattttagc gatcagcatc gcgtcgtcat ccgcgattac
     6961 tatgcaacag agtttcgaag cgggcactgc cctccggggc tcgccaagaa gaggaacggc
     7021 tgcatgccgc ccggacaggc gaagaagtgg cgcacgggcc acccgctgcc gcgggatgtc
     7081 atttactacg atctcccgcc cgcgctcgtg gtcaagatc
//
LOCUS       DASURG010000060         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1034320, whole genome shotgun sequence.
ACCESSION   DASURG010000060 DASURG010000000
VERSION     DASURG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034320"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /gene="pgk"
                     /locus_tag="VFY80_01010"
     CDS             complement(<1..708)
                     /gene="pgk"
                     /locus_tag="VFY80_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6006508.1"
                     /translation="MPILKMTEVDLRGKRVFIRVDFNVPLKDGAISDDTRIRAALPGI
                     RHALSQQARVMLTSHLGRPKEGEPTEADSLAPVARHLSTLLGMEVPLQRDWLDGVKVE
                     PGHAVLLENCRFNKGEKKNDDALARRMAALCDVYVNDAFGTAHRAEASTHGIAKFAPV
                     ACAGPLMAAEIDALTRALEKPARPMVAVVGGSKVSTKLTILAAFAAKVDQLIVGGGIA
                     NTFLLAAGMKIGKSLAEP"
     gene            complement(731..1744)
                     /gene="gap"
                     /locus_tag="VFY80_01015"
     CDS             complement(731..1744)
                     /gene="gap"
                     /locus_tag="VFY80_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6006509.1"
                     /translation="MSIKVAINGYGRIGRNILRAHYEGGKKHDIQIVALNDLGNAETN
                     AHLTRYDTAHGKFPGRVEVDGDAMVVNGDRIKVLAVRNPAELPWSALGVDVVLECTGL
                     FTSKEKASSHLKGGAKKVIISAPGGKDVDATIVYGVNHKTLKASHTVISNASCTTNCL
                     APMVKALHEKIGITHGLMTTVHAYTNDQVLTDVYHEDLRRARAATMSMIPTKTGAAAA
                     VGLVLPELNGKLDGFAIRVPTINVSIVDLTFTAARATSVEEVNAAMKAAAEGELKGVL
                     EYSKAPLVSVDFNHNAASSIFDATLTKVTGGTLVKALAWYDNEWGFSNRMLDATVALM
                     SAK"
     gene            complement(1840..>2230)
                     /locus_tag="VFY80_01020"
     CDS             complement(1840..>2230)
                     /locus_tag="VFY80_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6006510.1"
                     /translation="AIDHGGYVLSEAPGDAPRCVLIATGSEVALAMEAQKRLAADGVP
                     VRVVSMPSTSVFDRQDANYRDQVLPRGVPRVAVEAGVGDYWRKYVGLDGAVVGVDRFG
                     ESAPAGEVFRFFGFTPENVVQTVRKLL"
BASE COUNT          381 a          722 c          689 g          438 t
ORIGIN      
        1 gggctcggcg agcgacttac cgattttcat gccagccgcg agcagaaagg tgttcgctat
       61 gccgccgccg acaatcaatt gatcgacttt cgccgcgaac gccgcgagga tcgtgagctt
      121 ggtcgagact ttcgagccgc ccacaacggc gaccatcggc cgggccggtt tttcaagggc
      181 gcgcgtgagc gcatcgatct ccgccgccat cagcgggccc gcacaggcaa ccggcgcaaa
      241 tttcgcgatg ccgtgcgtcg aggcctccgc gcggtgcgcc gtgccgaacg catcattcac
      301 gtagacgtcg cacagtgcgg ccatgcggcg cgcgagcgcg tcgtcgttct tcttctcacc
      361 cttgttgaag cggcagtttt cgagcaggac ggcgtgcccc ggttccacct tgacgccgtc
      421 gagccagtcg cgctggagcg gcacctccat cccgagcagc gtcgagagat ggcgcgcgac
      481 cggcgccagg gaatcggcct cggtcggctc gccctccttg gggcgcccaa gatgggaagt
      541 gagcatgacg cgcgcctgct gcgacaacgc gtggcggatg cccggcagcg cggcgcgtat
      601 gcgtgtgtca tcgctgatcg cgccatcctt gagcggcacg ttgaagtcca cgcggatgaa
      661 gacgcgtttt cccctgaggt cgacctcggt catcttgagg atgggcatag ctcttcccgt
      721 tcggctttcc ctacttggcg ctcatcagtg cgaccgttgc atcgagcatg cggttgctga
      781 agccccactc attgtcgtac caggcgaggg ctttgacgag cgtgccgccc gtcaccttcg
      841 tcagcgtggc atcgaagatg ctggatgccg cgttgtgatt gaagtccacc gagacgagcg
      901 gcgccttgct gtactcgagc acacccttga gctcaccttc cgctgcagcc ttcatcgccg
      961 cgttcacttc ctcgacgctg gttgcgcgcg ccgccgtgaa cgtgagatcg acaatcgaca
     1021 cgttgatcgt cggcacgcgt atcgcgaagc cgtcgagctt gccgttaagc tcgggcagca
     1081 cgagcccgac cgccgccgcc gcaccggtct tggtcggaat catcgacatc gtcgccgcgc
     1141 gcgcgcgccg cagatcctcg tgatagacgt ccgtcaacac ctggtcgttg gtataggcgt
     1201 gaaccgtcgt catcagccca tgcgtgatgc cgatcttctc gtgcagcgcc ttgaccatgg
     1261 gcgcaaggca gttcgtcgtg cacgaggcat tggaaatgac ggtgtgactc gccttgagcg
     1321 tcttgtgatt gacgccgtag acgatggtcg catcgacgtc cttgccgccg ggggcggaaa
     1381 tgatcacctt cttcgctccg cccttgagat gggaggaagc cttctccttg ctggtgaaaa
     1441 ggccggtgca ttcgagcacg acatccacac cgagcgcgct ccaaggcagc tcggcaggat
     1501 tgcgcacggc gaggaccttg atgcggtcgc cattaaccac catggcgtcg ccgtccacct
     1561 ccacccgtcc tggaaacttg ccgtgggcgg tgtcgtaacg cgtcaggtgc gcattggttt
     1621 ccgcgttccc gagatcgttc agggcaacga tctggatatc gtgtttctta ccgccttcgt
     1681 agtgcgcgcg caggatgttg cgcccgatgc ggccatagcc attgatggca accttgatgc
     1741 tcatcgcagt tctccgattg gaagatagat gaaggttgga tctgcacctc tatgacaggg
     1801 gcggaccggc ttgcgttctc gcaagctccg ggcacgtcct tacagcagtt ttcttacggt
     1861 ctggaccacg ttctccggcg tgaaaccaaa gaagcggaac acttctccag caggtgccga
     1921 ttcgccgaaa cgatcgacgc cgaccactgc accgtcgagt cccacgtatt tgcgccagta
     1981 atcgcccacg ccggcttcga cggcaacacg cggcacgccg cgcgggagaa cttggtcccg
     2041 atagttggcg tcctgccggt cgaaaacgga tgtgctcggc atggatacga cgcgcaccgg
     2101 gaccccgtcg gctgcgagcc gcttctgagc ctccatggcg agcgcgactt ccgaacccgt
     2161 cgcgatgagc acgcaccgcg gtgcgtcacc cggcgcttcg gagagcacat acccgccgtg
     2221 gtcaattgca
//
LOCUS       DASURG010000061         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_830266, whole genome shotgun sequence.
ACCESSION   DASURG010000061 DASURG010000000
VERSION     DASURG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830266"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1474
                     /locus_tag="VFY80_01025"
     CDS             44..1474
                     /locus_tag="VFY80_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6006511.1"
                     /translation="MWKAVLTALVALLAACEQRQPAVSDPYANVELKSPPAKVTAERN
                     KGSFAEGAALSMGPELKPLDSAPSKTVRLDVTHRIIDIMPGVKFAAWTFGDQVPGPTI
                     RARVGDRIKFVMTNRSDEPVGPPEIVTAPMMHSMDFHAAMVSPQDKYRSIAPGQTIEF
                     EFTLNYPGVFMYHCGTPMVLEHIASGMYGAVVVEPRNGYPTKVNREYVVIQSEFYTRP
                     DPQNRKIDGVPVHVLDTERLRASQPNHTVFNGVHDGMIKKPLAANPGERVRLYLLNVG
                     PSKTSSFHVVGTIFDRVWFEGNPDNQFRGMQTVLLGSSNSAIVEFVIPEPGSYVMVDH
                     HFANASQGAVGLIEAGVKAEEASAEHHNLPASANPADPEALKAKTSFESKCVACHAIS
                     GGEGKKIGPDLLGVTKRRNEDWLARFLSSPEQMIATDPTAKALLEKYKVPMPNQNLSE
                     DEIRGFIKYFHFLDGTEQAPAKAAAH"
     gene            complement(1514..1939)
                     /locus_tag="VFY80_01030"
     CDS             complement(1514..1939)
                     /locus_tag="VFY80_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006512.1"
                     /translation="MLIRGSCHCANIAFTLRVEPDPAEIPARACTCSFCAKHGGLWTS
                     YPGGTLEVSIEDAAQVSNYMFGTRTAEFHICTRCGVVPVVTSLIEGRIYAVVNVNTFE
                     GIDPALIRRTPASFDGEGEGPRLARRKRNWIADVRMAAK"
     gene            complement(1959..2933)
                     /locus_tag="VFY80_01035"
     CDS             complement(1959..2933)
                     /locus_tag="VFY80_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006513.1"
                     /translation="MDRSITKRCTVLLMLAAGAAAAHAQAPAYPTKPIRIIIALAPGG
                     GVDTTGRFIGQKLNEAWGQPVVADNRPGAGGSIAAELVARAAPDGHTLLMTSSGVTIT
                     PSLMKLGYDAKTDLLPVTQAVISPGVMVVHPSLPVKSVKELIVFAKGKPGELFFSSSG
                     QGSAQHLTIELFCQMTGLSMTHVPYKGTAPSITDVVAGRIALTVASVISTRPMFTTGK
                     LRALAVVGTKRTPALPEYPTVAESGVPGFGVDQWYGLFLPGGTSQAIAVKLQEAISRA
                     LLDPATRKTLLAQGLDAVGSSQGEFAKLYAAELEKWAKVVRSVGLQAK"
     gene            complement(3365..3859)
                     /locus_tag="VFY80_01040"
     CDS             complement(3365..3859)
                     /locus_tag="VFY80_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX6006514.1"
                     /translation="MRNDAAATGLSYIALAVYLALCFAVAAVGGAVTASSVATWYANL
                     TKPAFNPPDWVFGPVWTMLYAVIAIAGWRIWLRRREPGAGIALGAWSLQLVLNLAWSL
                     IFFGARMIGAALLEIVVLLAVILFTTVYFWRIDQLAGALFVPYAMWVAFATALNAALW
                     QLNP"
     gene            4040..4354
                     /locus_tag="VFY80_01045"
     CDS             4040..4354
                     /locus_tag="VFY80_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006515.1"
                     /translation="MKLLIAGLTTGLLFTATPALADRWKDESGHGWRRSHQETRHWDQ
                     RWERGRHHSDRHRHWSRHRHHHHYYPRHHSYRNYSYSYSYPAPSAYVGTPSIYFSWSS
                     GY"
BASE COUNT          878 a         1420 c         1329 g          792 t
ORIGIN      
        1 attccattgt cttttcgatt tcaattccct caaggaacgc agcatgtgga aagccgtcct
       61 gaccgctctc gtcgcgctcc tcgccgcgtg cgagcagcgt cagccggcgg tgtccgatcc
      121 ctatgcaaac gtcgaactga aatccccgcc cgccaaagtc accgccgagc ggaacaaggg
      181 ctctttcgcg gaaggcgccg cgctcagcat ggggcctgag ctgaagccgc tcgactctgc
      241 tcccagcaag acagtccggc tcgacgtcac gcatcgcatc atcgacatca tgccaggcgt
      301 caaatttgcc gcctggactt tcggtgacca ggtgcccggg cccacgatcc gcgcgcgcgt
      361 cggggaccgc atcaagttcg tcatgacgaa ccgcagcgac gagccggtcg gtcctccgga
      421 gatcgtgacg gcgccaatga tgcactcgat ggacttccac gccgcgatgg tatcgccgca
      481 ggataagtac cgctcgattg cgcccggcca gacaatcgag ttcgagttca cgctcaatta
      541 ccccggcgtg ttcatgtacc attgcgggac gccgatggtg ctcgagcaca ttgcatccgg
      601 catgtacggc gcagtcgtgg tggagccgcg caatggctat ccaaccaaag tcaatcgtga
      661 atacgtcgtc atccagagcg agttctatac caggccggat ccacaaaacc gcaagatcga
      721 cggcgtgccc gtgcacgtgc tcgacaccga acggctgcgc gcctcgcagc cgaaccacac
      781 cgtcttcaac ggcgtgcacg acggcatgat caagaagccg ctcgctgcaa atcccgggga
      841 acgcgtgcgg ctctatctcc tcaacgtcgg cccgagcaag acgtcgagct tccacgtggt
      901 cggcacgatc ttcgatcgcg tgtggttcga aggcaatccc gacaatcagt tccgcggcat
      961 gcaaaccgtg ctgctcggct cgtcgaacag cgcgatcgtg gagttcgtga ttcccgagcc
     1021 cggctcgtac gtgatggtgg atcaccattt cgccaatgca tcgcagggcg ccgtgggcct
     1081 catcgaggct ggcgtgaaag cggaagaagc ttcggccgag caccataacc tgccggcatc
     1141 cgcgaatccc gccgatccgg aagccctcaa ggcgaaaacg agcttcgaat cgaagtgcgt
     1201 cgcctgccac gcgatctcgg gtggagaagg caagaagatc gggccggacc ttctcggcgt
     1261 caccaagcgg cgcaacgaag actggctcgc acgcttcctc agctcgccgg agcagatgat
     1321 cgcgaccgac cctaccgcca aggcgctgct cgagaaatac aaggtgccga tgccgaacca
     1381 gaatctttcc gaggacgaga tccgcggctt catcaagtat ttccacttcc tcgacgggac
     1441 ggaacaggcg ccggcgaagg ctgcggcgca ttagaagaca ataccaacgg accgacgaga
     1501 gcctcgcgcg cgcttatttc gcggccatcc tgacgtcggc gatccagttg cgctttctcc
     1561 gggcaagccg cggaccttcg ccttcgccat cgaagcttgc cggtgtgcgg cgtatgagcg
     1621 cgggatcgat cccttcgaaa gtgttcacgt tcacgacagc gtaaattcgg ccctcgatga
     1681 gactcgtgac gaccggcacg acgccgcatc gggtgcagat gtggaattcc gcagtgcgcg
     1741 ttccgaacat gtagttcgac acctgggcgg catcctcaat cgatacttcg agcgtgcccc
     1801 cgggataaga ggtccacagc ccgccgtgct ttgcgcagaa cgagcaggtg caggcgcggg
     1861 ccgggatttc ggccgggtcg ggttcgacgc gcagcgtgaa agcgatgttg gcgcagtgac
     1921 agcttccccg gatcagcatg cgcggaccgc gggttacctt actttgcctg caggcccacg
     1981 ctgcgcacga ccttcgccca tttttcgagc tcggctgcgt acagtttcgc aaactccccc
     2041 tgcgaacttc cgaccgcatc cagcccttgc gcgagcagcg tcttgcgtgt cgcgggatcg
     2101 agcagagcgc gcgatatcgc ctcctgcagt ttcacggcga tcgcctggct cgtgccgccc
     2161 ggaagaaaga gcccatacca ctgatcgacg ccgaatcccg gcacgccgga ttccgcaacg
     2221 gtgggatact cgggaagtgc cggcgtgcgc ttggttccca ctacagcgag cgcccgcagt
     2281 ttcccggtcg taaacatcgg acgcgtcgag attacgctcg cgacggtcag cgcgatgcgg
     2341 cccgcgacga cgtcggtgat gctcggcgcc gtacccttgt acgggacgtg cgtcatgctg
     2401 agccccgtca tctggcagaa gagttcgatc gtcagatgct gcgccgaccc ctggccggaa
     2461 gaggagaaga aaagttcacc cggcttcccc ttggcgaaca cgatcaactc cttgacgctt
     2521 ttcaccggta gcgagggatg gacgaccata acgcccggag agatcaccgc ttgggtgacg
     2581 ggcaggagat ccgtcttcgc gtcgtagccg agcttcatga ggctcggcgt aatcgtcact
     2641 ccggacgagg tcatgagcag cgtatggccg tccggcgcgg cgcgggccac cagttcagcg
     2701 gcgatcgatc cgccggcacc gggtcggttg tcggcgacga ccggctgtcc ccacgcctcg
     2761 ttcagcttct ggccgatgaa ccggccggta gtatcgacgc ctccgcccgg agcgagcgca
     2821 atgataatgc gtatcggctt ggtcggataa gccggcgcct gcgcatgagc cgccgctgct
     2881 cccgccgcga gcatgagcag gactgtgcaa cgttttgtga tcgaccgatc catggttccc
     2941 tccagtgacc ggcgaaagta tagaggatta tcgccgggtt cgagctggcg ggaggccagg
     3001 cgcgcggcgt gcggagcgcc tcgcggagcg cctggacggc accgaacgca ccgcgcttcc
     3061 tggatttttc gattgcccgg tggattgctg ggcgcgggag aggtcgggcg gcgcgcacca
     3121 caggatataa gtcggaagag ccatacagca tcacatcctt tgtagcgact atagcggtcc
     3181 ttcgtttcag aagtgtttcc gacccggccg ggtttgtaac gttccggagt cggcctttcg
     3241 cctcatagtc gaacgacacg gctcatgatc gacatgtgca tcacgcgcgc accatcgccc
     3301 cccgctgact tgtggggtgt tacacggctg aaacaaacag ttcgaggggc gctgcccgat
     3361 gcggttaggg gttgagctgc cacagcgctg cattcagcgc cgttgcaaat gcaacccaca
     3421 tcgcgtacgg aacaaagagc gcaccggcga gctggtcgat gcgccagaag tacacggtcg
     3481 tgaacaggat tacggcgagg agaacgacaa tttcgagcaa tgccgcgcct atcatgcggg
     3541 cgccgaagaa gatcagcgac catgcgagat tcagcacgag ctgcaggctc cacgcgccga
     3601 gtgcgatgcc cgcacctggc tcgcgtctcc gcagccaaat ccgccacccc gcaatggcga
     3661 tcacggcgta gagcatcgtc cacaccggtc cgaacaccca gtctggcgga ttgaatgcgg
     3721 gcttggtgag atttgcgtac caggttgcca cactgctcgc tgttactgcg ccgcccacag
     3781 cggcgactgc gaagcacaac gccagataga ccgccagcgc tatgtacgaa agaccggttg
     3841 ccgctgcgtc attacgcatt cagaacgccc atgtaaaagt gttttgcccg cggccggctt
     3901 ggcaacaatt tcttacaaca aacgatgcgt ggcttgcaaa cggatcgccg cttctcgcta
     3961 ctctaaccgt aacggcaatc ggcagcacat tccactccgg aatctgcccg gttatccgct
     4021 actcgaggag gctcgcgcca tgaaactctt gattgctgga ctgacgaccg gcttgctctt
     4081 cacggctaca cccgcattgg ccgatcgatg gaaagacgaa agcggccatg gatggcgcag
     4141 aagccatcag gaaacccggc attgggatca gcgctgggag cgtggtcgcc accactcgga
     4201 ccgccatcgc cactggagcc gccaccgcca ccatcaccac tattatcccc ggcaccattc
     4261 ctaccggaat tactcctact cctactccta tccggcgccg agtgcgtatg taggcacacc
     4321 gagcatctat ttctcgtggt cgagcggcta ttgaccgcaa gccctgaaac cgcgggcacg
     4381 ccggaatgcg cgcgcagaag ttggcgcggg ctacgagga
//
LOCUS       DASURG010000062         6507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1341696, whole genome shotgun sequence.
ACCESSION   DASURG010000062 DASURG010000000
VERSION     DASURG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341696"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..941)
                     /locus_tag="VFY80_01050"
     CDS             complement(51..941)
                     /locus_tag="VFY80_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6006516.1"
                     /translation="MRAFELLSAENARHAAALLAEHGPSARLLAGGTDLLVELKSAPA
                     TPRLIIDLSRAEDLKRIAVTEEGLSIGALVTHAQIMRSPLIRDGAPVLVDAAHAIGAA
                     QTRNLGTLGGNLVTGVPSMDSGPALFALDAQLTLVCASGRRRLPISDFFVGPRKTVLK
                     PDELLSEILIPAASLRKPARFEKVGLRKGQALALVNVAASMWIDWDTHSFVTPRIALG
                     AVAPTVMRAHSAEAFLEGQPVTLEAMAEAACLAAGEARPIDDFRASAWYRRDLVAVLA
                     RRALFAAHGTALRMHEAGIA"
     gene            complement(955..1824)
                     /locus_tag="VFY80_01055"
     CDS             complement(955..1824)
                     /locus_tag="VFY80_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6006517.1"
                     /translation="MEPQLLIPGVIFGLSIGSIFFLMAIGLSLCFGLMRIISLDQLLY
                     YSLGAYLTYTISVASGNVWLGMVVGVIVAGIASLAVERFVFPRIYERELTFSMITSFG
                     ILIGGVGVIKYFWGVVPRPVAIPTLMQINIAGTDVPIYRLMVIGIAALVFGGIWLFLN
                     RTIIGKAIRAGIENVDHVEGLGINVSRLFTITFIIAGTLSGLAGGLHAPLIMVDPQMG
                     LEVLAFVFMVVIIGGLGSIKGTLVSAFIVGQVVSLGSLIYAPLAQMAPFAIMLLILLI
                     KPTGLYGSALLKR"
     gene            complement(1824..2810)
                     /locus_tag="VFY80_01060"
     CDS             complement(1824..2810)
                     /locus_tag="VFY80_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6006518.1"
                     /translation="MTNAIKWWWGLLLAFGLIALARPFVPLPFIIEVLIFSIYTMGCN
                     FLLGRVGFISFGQPAYLAAGAYGTAFYLFYFGTNPYLGILVGIASGLAVAAIVGPLFV
                     RLRSDYFALVNLALTVIIYYLMQKVLAGVTKGDNGLWFLTRMTSTPLLDIATPKGFFI
                     FALLIAFGLWAFYKYLDDSIFGACCLATKQNEDKLEFLGYSNFRVRLMAFLIANTTTA
                     LAGAMYAVYLGFVSPEISSPHRAPDPVVVTILGGTGTLYGPIVGAVAYTGMKDVISKL
                     IGNWELFIGFLLVFIMLAGEKGIWGTAQPYLEKLFVRRKAAASVGVEAEARR"
     gene            complement(2824..3540)
                     /locus_tag="VFY80_01065"
     CDS             complement(2824..3540)
                     /locus_tag="VFY80_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6006519.1"
                     /translation="MPPDSGVAAMTERILAVERLNVSYGASKVLFELSMSVRPCEIVA
                     CVGRNGAGKSTLLKTIAGFLKPDSGTVKSGEANLVGLRSYEIARRGIKYIPQDKKVFS
                     DLTVRENLELASYASKDYNWEPITGYFPKLKQLMDRKAGYLSGGERQMLMIGRALLGS
                     PKLLLVDEPAEGLAPSIVSHLREVLRQLSANTALVLVEQNLPLVCAIAAKVYAIKEGR
                     IVAELADRDSITPQECERYL"
     gene            complement(3530..4321)
                     /locus_tag="VFY80_01070"
     CDS             complement(3530..4321)
                     /locus_tag="VFY80_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6006520.1"
                     /translation="MNDAGAEIIRAQGLTKRFGAQAAVDRVDFTLRKHEVAGIIGSNG
                     AGKTTLFNLITGYYLPDEGVILHKGIDVTRMTPQQRVAAGMMRTFQLTSTFDNLSVTD
                     NLVLAFFRAYRKPSLLGLIFNTCKRYRNEPKILAILETFDLASLRDRQVKNLSLGEKR
                     RLEIAMAVLVEPELLLLDEPLAGLAESEIKSVLEVLRRHIGKRTILIVEHKISHVKDF
                     VQRLTVMHEGRIIAEGDYEACLRDPEVRRSYWQIDAAAEERPHAA"
     gene            complement(4397..5635)
                     /locus_tag="VFY80_01075"
     CDS             complement(4397..5635)
                     /locus_tag="VFY80_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6006521.1"
                     /translation="MKKLVSLIAAAALSVLAGQAGAASPQKVKIGILLPLTGTFAAVA
                     ETQKEGALLAVDVINKRGGLNMPGGKVMLEGVVQDDEAKQDIGVRRYRYLVSEGVKGV
                     GGQTWAPLAYAVNAIVSKEPMPYFPVCVMAKEAFEKGKLAGSTFATAYSPWSVGYMAG
                     QAAIKTLGKKRIFFLARADSWGWDMRDGAYAAAKELGAEIVGYDEVSLGTPDFTTVLQ
                     KVRQAKPDVFISAQFAADAVALLKQVHQAGLGKEMTIFNAFITNVVAKGLPPEALEGV
                     YAMHYFYYDLANFADKEVAKSAQEFTDLYRAKYKRPPDAYATIAYIAYMEMFRGFEAA
                     KSFEPQKVSAALMANKGQFNSVKGPAYWRQDHSAVYKHAAFLVRGKGAKDQKTEWDLF
                     SVMGSVGGEQVMPSLKSLGY"
     gene            complement(5794..>6507)
                     /gene="panC"
                     /locus_tag="VFY80_01080"
     CDS             complement(5794..>6507)
                     /gene="panC"
                     /locus_tag="VFY80_01080"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6006522.1"
                     /translation="EQRAACKVVSIFVNRLQFGPREDFDRYPRTLEADCAKLRAVGVN
                     VVFAPDEKEIYPEPQTFLVEPSDLQHILEGEHRPGHFRGVATVVLKLFNLVMPHSAVF
                     GKKDYQQYMVLRDMVRQFALPIEILPAETVRAADGLALSSRNAYLSAQERREAPRLYA
                     LLRQAHDEVAGGARDFQRIELHSMSELANHGWKPDYVAVRRQADLQPPTPEDHDLVVL
                     AAARLGRTRLIDNVEVFLR"
BASE COUNT         1266 a         2046 c         2021 g         1174 t
ORIGIN      
        1 cggaaccttt accgtatgcg ctctgccgtt cacccgcaac gtgatcgcgt tcatgcaatc
       61 cccgcctcgt gcatgcgcag cgcggtgccg tgtgcagcga agagcgcacg gcgcgcaagg
      121 acggcgacga gatcgcgccg ataccacgcg gacgcacgga aatcgtcgat cggccgcgcc
      181 tcgcccgccg caaggcacgc cgcctcggcc atcgcttcca aagtgaccgg ttgaccttcg
      241 agaaacgctt ccgcgctgtg cgcgcgcatg accgtcggcg ccacggcacc gagtgcgatg
      301 cgcggcgtga cgaagctgtg cgtatcccag tcgatccaca tgctcgccgc aacgttgacg
      361 agcgcaagcg cctgtccttt gcgcaagcca accttctcga aacgggccgg cttgcgaagg
      421 ctcgcggcgg gaatgagaat ttcggagagg agttcgtccg gcttgaggac agtcttccgc
      481 ggcccgacga agaaatcgga gatgggcagt cgacggcgtc ccgaagcgca cacgagcgtg
      541 agctgggcgt cgagcgcaaa gagcgcgggc ccgctatcca tggatggcac gcccgtgacg
      601 agattgccac cgagcgtccc gagattgcgc gtctgcgctg cgccgatggc atgcgcggcg
      661 tccacaagca ccggcgcccc gtcccggatg agcggcgagc gcatgatttg agcgtgcgtg
      721 acgagcgcgc cgatgctcaa gccctcttcc gtcaccgcga tgcgcttcaa gtcttcggca
      781 cgcgaaagat cgatgatgag ccgcggcgtg gccggcgcgc tcttgagttc aacgagcaga
      841 tcggtgccgc ccgcaagcag gcgcgcagac ggcccgtgct cggcgagcag cgctgcggcg
      901 tgccgggcat tttccgcact caacagttcg aaggcgcgca tgacggcttt gagatcagcg
      961 cttcaggagc gcactcccgt acaggccggt cggcttgata aggagaataa gcagcatgat
     1021 ggcgaacggc gccatctgcg cgagcggcgc atagatgagc gaaccgagcg acaccacctg
     1081 tccgacgatg aaggcggaga cgagcgtccc cttgatactg cccagccccc cgatgatcac
     1141 aaccatgaac acgaatgcga gcacctcgag gcccatctgc ggatcgacca tgatgagcgg
     1201 cgcgtgcagg ccgccggcaa ggcccgagag cgtgcccgcg atgatgaatg tgatggtgaa
     1261 gagccgcgag acattgatgc ccagcccctc cacgtggtcc acgttctcga tgccggcgcg
     1321 gatcgccttg ccgatgatgg tccggttgag gaagagccag atccctccga agaccagcgc
     1381 ggcgatgcca atcaccatca ggcgatagat cggcacgtcg gtgcctgcga tgttgatttg
     1441 catgagcgtc gggatggcaa ccgggcgtgg aaccactccc cagaagtact tgatgacacc
     1501 gacgccgccg atcaggatgc cgaaggaggt gatcatgctg aacgtgagtt cgcgctcgta
     1561 gatgcgcgga aagacgaaac gctccactgc aaggcttgca attcccgcga cgatcacccc
     1621 cacgaccatc ccaagccaca cgttgccgct cgcgacgctg atcgtgtagg tgagataggc
     1681 gccgagggaa tagtagagaa gctgatccag gctgatgatg cgcatgaggc cgaagcacag
     1741 tgagagcccg atcgccatca ggaaaaagat cgacccgatg gagagcccga aaatgacgcc
     1801 gggtatcaga agttgaggct ccactatctg cgcgcctccg cctcgacgcc tacggaagcc
     1861 gcggccttcc gtcggacgaa caacttctcc aggtacggct gggcggttcc ccagatgcct
     1921 ttttcaccgg caagcatgat gaagaccaga aggaaaccta tgaagagctc ccagtttccg
     1981 atcagcttgc tgatcacatc cttcatcccg gtataagcga ctgcccccac gatcggcccg
     2041 tagagcgttc ctgtcccccc gagtatcgtc acgaccaccg gatcgggtgc gcgatgcggg
     2101 ctggagatct cggggctcac gaagcccaga tacacggcgt acatcgcgcc ggcgagcgcg
     2161 gtcgtcgtat tggcgatgag aaacgccatg agccgcacgc ggaaattgct gtagcccagg
     2221 aactcgagct tgtcctcgtt ctgcttggtg gcgagacagc aggcgccgaa gatggagtca
     2281 tccaggtact tgtagaacgc ccatagtccg aatgcgatca gcaaggcgaa tatgaagaat
     2341 cccttggggg tggcgatatc gagcaacggg gtcgaagtca tgcgcgtcag gaaccagagt
     2401 ccgttgtctc ccttggtgac cccggcgagg accttctgca tgaggtaata gatgatcacc
     2461 gtaagcgcga gattcacgag cgcgaagtag tcgctcctca atcgcacgaa caaagggccc
     2521 acgattgcgg cgaccgcgag acccgaagcg atgccgacga ggataccgag atagggattg
     2581 gtgccgaagt aaaagagata gaaggccgtg ccgtaggcgc cggccgcgag gtatgccggc
     2641 tgcccgaagg aaatgaatcc cacccgaccg agcaggaaat tgcagcccat ggtgtagatc
     2701 gagaaaatca ggacctcgat gatgaagggc agcggcacga aaggccgcgc gagcgcgatc
     2761 aatccgaacg cgagaagaag gccccaccac catttgattg cgtttgtcat cgtcggtgag
     2821 gcgctagaga taccgctcgc attcttgcgg cgtgatggag tcgcgatccg ccagctctgc
     2881 gacaatacgg ccctccttga tcgcatacac cttggccgca atcgcgcaga cgagcggcag
     2941 gttctgctcc acgagcacga gcgcggtatt cgcgctcagt tgtcgcagca cttcccgcag
     3001 gtgggacacg atgctcggag caagcccttc ggccggctcg tccacgagca gcagtttcgg
     3061 gctgccgagc agcgcccgcc cgatcatcag catctgccgc tcgccgccgc ttagatagcc
     3121 ggccttgcgg tccatcagtt gcttgagctt cgggaaataa ccggtgatgg gctcccagtt
     3181 gtaatccttg ctcgcatagc tcgcgagctc cagattttcc cgcacggtga gatcggagaa
     3241 gaccttcttg tcttgcggaa tgtacttgat gccccggcgg gcgatctcat acgacctcag
     3301 gccgacgaga ttcgcctccc ctgacttcac ggttccggag tcgggcttca ggaatcccgc
     3361 gatggtcttg agcagcgtgc tcttgccggc cccgttgcgg ccgacacacg ccacgatctc
     3421 gcacggacga acgctcatgc tgagctcgaa cagcaccttc gatgcgccat acgacacatt
     3481 gaggcgctcc acggcgagaa tgcgctccgt catcgcggca accccgctgt caggcggcat
     3541 gcggtctctc ctccgcggcg gcgtcgatct gccagtagct ccttctcacc tcgggatcgc
     3601 gcaggcacgc ctcgtagtcg ccctcggcga tgatccgtcc ttcgtgcatg accgtgaggc
     3661 gctgcacgaa gtcctttacg tgagagatct tgtgctcgac gatgaggatg gtgcgcttgc
     3721 cgatgtgccg gcgcagcacc tcgagcacac tcttgatctc cgattccgca agacccgcga
     3781 gcggctcgtc gagcagcagc agttccggct cgaccagcac tgccatggcg atttcgagtc
     3841 ggcgcttttc ccccaggctc agattcttga cctgccggtc gcgcaatgac gcgagatcga
     3901 aggtttccag tatggcgagt atcttcggtt cgttgcggta gcgcttgcag gtgttgaaaa
     3961 tgaggccgag gagcgatggc ttgcgatagg cccggaaaaa cgcgagcacc aggttgtccg
     4021 tcacgctcag gttgtcgaac gtggaggtca actggaaggt gcgcatcatg cccgccgcca
     4081 cccgctgctg cggtgtcatg cgcgtgacat cgatcccctt gtgcaggatg acgccctcgt
     4141 ccggcaggta gtagccggtg atgagattga acagcgtcgt cttgccggcg ccgttcgagc
     4201 cgatgatgcc tgccacttca tgcttccgca gcgtgaaatc gacccggtcc acggcagcct
     4261 gagcgccaaa gcgcttggta agcccctgtg cccggatgat ttcggcgcct gcgtcattca
     4321 tggccagctc tgtttcgggc gccgcttcga caaaggaacg aaactcgcca aggcgtgccc
     4381 ttcctttccc cggatcctag tagccaagag acttgagcga gggcatgacc tgctcgccgc
     4441 cgacggagcc catgaccgag aagagatccc actcggtctt ctggtccttc gcgccttttc
     4501 cgcgcaccag gaaggcggcg tgcttgtaca cggccgagtg gtcctgacgc cagtacgcgg
     4561 gacctttgac ggaattgaac tgccccttgt tcgccatcag cgcggcggac accttctgcg
     4621 gttcgaacga cttggcggcc tcgaagccgc ggaacatctc catgtacgcg atatacgcga
     4681 tcgtcgcgta ggcatcaggc ggccgcttgt acttcgcgcg gtaaaggtcg gtgaattcct
     4741 gcgcgctctt cgcaacctct ttgtccgcga aattggcgag gtcgtagtag aaataatgca
     4801 tggcgtacac gccttccagc gcttccggcg gcaggccctt cgcgacgacg ttggtgatga
     4861 aggcgttgaa aatggtcatt tccttgccga gacccgcctg gtgcacttgc tttagcagcg
     4921 ctaccgcgtc ggcagcgaac tgcgcggaaa tgaatacatc cggcttcgcc tggcgcactt
     4981 tctgcagcac cgtcgtgaaa tccggcgtgc cgagggagac ttcatcgtag ccgacgattt
     5041 cggcgcccag ctccttggcg gcggcatacg cgccatcgcg catatcccat ccccagctgt
     5101 cggcgcgcgc gaggaagaag atgcgcttct tgcccagcgt cttgatcgcg gcctggccgg
     5161 ccatgtagcc taccgaccac gggctgtagg ccgtggcgaa cgtggagccg gcgagtttgc
     5221 ccttttcgaa cgcttccttc gccatcacac acaccgggaa gtagggcatc ggctccttgc
     5281 tcacgatggc gttgacggcg taggcgagcg gcgcccaggt ctggcctccg acgcccttca
     5341 cgccttccga gacgaggtat cggtagcgac gcacgccgat gtcctgtttc gcttcgtcgt
     5401 cctgcaccac gccttcgagc atgaccttcc cgcccggcat gttgagcccg ccgcgcttgt
     5461 tgatgacatc caccgcaagc agtgcgcctt ccttctgcgt ttccgcgacc gcggcaaacg
     5521 ttccggtgag aggaagcagg atgccgatct tgaccttctg gggactcgct gcccccgctt
     5581 gaccggcgag aacggataat gctgcagcag cgatcaggct tacgagcttc ttcatgactc
     5641 cctccgcagg cgaaagaaac cggacaatac gtcgcgcgcg cgagcatcgc gcgtcgggac
     5701 gcggtgcaat tctcgccgct gggagaagcc ccctgtttga tataggtcaa caaacaacgc
     5761 tgccgcagcg cggccgcgcc ggggtgcggg aaattacctg aggaaaactt cgacgttgtc
     5821 gatcaggcgc gttctgccga ggcgggcggc ggcgagcacg acaagatcgt gatcttcggg
     5881 cgtcggcggc tgaagatccg cttggcggcg cacggcaacg taatcgggct tccatccgtg
     5941 gttcgcaagc tcggacatgc tgtgcaattc gatgcgctga aaatcgcgcg cgccgccggc
     6001 aacctcatca tgcgcttggc gcagcagcgc atagagccgc ggcgcctcac ggcgctcctg
     6061 tgcggagaga tacgcattgc gcgacgagag ggcaagaccg tccgctgcac gcaccgtctc
     6121 cgcggggagg atttctatcg gcagcgcgaa ctgacgcacc atgtcgcgca gcaccatgta
     6181 ttgctggtag tccttcttgc cgaacaccgc ggaatgcggc atcacgaggt tgaacagctt
     6241 cagcacgacg gttgctacgc cccggaagtg accggggcgg tgctcgcctt ccaggatgtg
     6301 ctgaagatcc gaaggctcca caagaaacgt ctgcggctcg ggatagattt ctttctcgtc
     6361 cggcgcaaag acgacgttca cgccgaccgc gcgcagcttg gcgcagtccg cctccagcgt
     6421 gcgcggatac cggtcgaaat cctcgcgcgg gccgaactgc aggcgattga cgaagatgct
     6481 gacgaccttg cacgccgcgc gctgctc
//
LOCUS       DASURG010000063         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_728913, whole genome shotgun sequence.
ACCESSION   DASURG010000063 DASURG010000000
VERSION     DASURG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728913"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFY80_01085"
     CDS             complement(<1..731)
                     /locus_tag="VFY80_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX6006523.1"
                     /translation="MQGVALRSVIAVLAAALLIAGCAAPRRAPVTERAPQAKPTPPLA
                     PAPAKKPAVSPESRPAFHTVKKGETLYSIALEYGLDYKDIAAWNSVDPGRVQVGQQLR
                     LTPPDAPVSAAPLKSAPPTIEARPLGVPSTAPGSSVAAPGGTVVKSEPRGVRVPYSDQ
                     TYAQLARLKPEEAAAAKPPEAAPPKPEAAPAPAGMAWTWPAKGKVIGTFNGSTTKGIV
                     IAGKLGQPVVASAAGKVIHSGTGIRG"
     gene            complement(739..1401)
                     /locus_tag="VFY80_01090"
     CDS             complement(739..1401)
                     /locus_tag="VFY80_01090"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX6006524.1"
                     /translation="MTSSRHDGIGMTSQRTRLRMVERLRESGIRDEVVLAAMSEVPRH
                     LFIDEALATRAYDDCSLPLGFGQTISSPYIVARMTELARGGRELGKVLEIGTGCGYQT
                     AVMARIAKEVYSIERLAPLLARARRTLREVRVTNARLRHGDGLRGIPELAPFDSIIMA
                     AAATHVPEVLREQLTAGGRMVLPMASKSGQQLCVIERTTQGYTEKRMDEVKFVPLLPG
                     VG"
     gene            complement(1398..2138)
                     /gene="surE"
                     /locus_tag="VFY80_01095"
     CDS             complement(1398..2138)
                     /gene="surE"
                     /locus_tag="VFY80_01095"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX6006525.1"
                     /translation="MRILISNDDGYFAPGIAMLQEALTDVAEVTVVAPERDRSGASNS
                     LTLDRPLTVRRSQNGFYYVNGTPTDCVHLAVTGLLEALPDVVISGINHGANMGDDTIY
                     SGTVAAATEGFLLGIPSLAISLVAEGGDHYETAARVAVELVQRFSSSPLAQAVLLNVN
                     VPDVAYERLQGREVTRLGKRHKAEPVVKSTTPRGDVVYWIGAAGGAQDAGEGTDFHAV
                     AKHAVSITPLQMDLTRYSQLEVVRKWLQ"
     gene            complement(2301..>3262)
                     /locus_tag="VFY80_01100"
     CDS             complement(2301..>3262)
                     /locus_tag="VFY80_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6006526.1"
                     /translation="AQVDRDTGDRQLIPDARTARRFTRASPACTMRSFDRPRGGALSA
                     SDAKAFRAACIQLNSGNDPQANLETVRRLARAAAAGADFLLLPEYALLLDGSGRVMRE
                     RAFDAEGGEALAALQALAAELGKWLLVGSLTVRTDEARIANRSYLLSSDGGVVATYDK
                     IHMFDATLPDGTVIRESSAYRPGAHAIIADIPWGRIGLTVCYDLRFPALFRSLAQAGA
                     QFITVPSSFQRQTGSAHWHALLRARAIENGCYILAPAMCGDHPGRRQTYGHSLIVDPW
                     GEILAEAGEDVGVVYADIEPARVARVRGMIPSLEHDRSFALSA"
BASE COUNT          557 a         1098 c         1026 g          581 t
ORIGIN      
        1 ccgcgtatgc ccgtgccgct gtgtatcacc ttgcccgctg cgctcgctac caccggttga
       61 cccagcttgc cggcgatgac aatacccttg gtggtgctgc cgttgaacgt accgatcacc
      121 ttgcccttgg caggccacgt ccatgccatc ccggccggcg cgggcgccgc ttcgggcttg
      181 ggcggtgccg cctccggggg cttagccgcc gcagcctctt ccggtttcaa ccgtgcaagc
      241 tgtgcatagg tctgatccga atacggcacg cgcacacctc tcggctcgct cttcacgacg
      301 gttccgcccg gtgctgcgac actggacccc ggcgcggtgg acggtacccc cagcggccga
      361 gcctcgatag tgggcggtgc cgatttgagc ggggccgcgc taaccggggc gtccggcggc
      421 gtgagccgca gctgctggcc gacctgcacc cttccaggat ccacgctatt ccaggcagcg
      481 atgtccttgt agtcgagacc gtactccaga gcaatgctgt agagcgtctc tcccttcttg
      541 acggtatgga acgccgggcg cgactcaggg gaaacagcgg gctttttggc gggcgccggc
      601 gccagcggtg gtgtcggttt ggcttgcggc gcgcgttccg tcacgggtgc acgccgcggc
      661 gctgcgcaac ctgcgatgag cagcgcggcc gccagcactg caatcacgga cctcaacgca
      721 acgccctgca cggtggtgtc accccacacc ggggagcaac ggcacgaact tgacttcgtc
      781 cattctcttt tccgtatagc cctgcgtcgt gcgttcgatg acgcatagct gttgtccgct
      841 tttgctcgcc atgggcaaaa ccattctacc accggccgtg agctgctcac gcagcacttc
      901 gggcacgtgc gttgctgccg cagccatgat gatcgagtcg aagggcgcga gttccggtat
      961 tccgcgcagg ccgtcgccat ggcgcaggcg cgcgttcgtg acccgaactt cgcgcagagt
     1021 gcgccgcgcc cgtgcgagca gcggcgccag ccgctcgatg gaatagactt ctttcgcaat
     1081 gcgggccatc accgccgtct ggtagccaca gccggtcccg atttcgagca ctttgccgag
     1141 ttcgcgcccg ccgcgcgcaa gctcggtcat gcgcgcgacg atataagggc tcgagatcgt
     1201 ctgtccaaag ccgagcggga gcgagcaatc atcgtaggct cgcgttgcaa gcgcttcgtc
     1261 aatgaacaga tgccgcggca cctcgctcat ggcggcaagg acgacttcgt cgcgtatccc
     1321 ggactcgcgc agccgctcca ccatgcgcag ccgcgtgcgc tgcgaagtca tgccgatgcc
     1381 atcgtgtctg gaagaggtca ttgcagccac ttcctgacca cttcgagctg ggaatagcgt
     1441 gtgaggtcca tctggagcgg cgtgatcgac accgcatgct ttgctacagc gtgaaaatcc
     1501 gtgccctcac ccgcgtcttg cgcacccccc gccgcaccga tccagtacac cacgtccccg
     1561 cgcggcgttg tggatttcac cacgggctcc gccttgtgac gcttgccgag ccgtgtcacc
     1621 tctctgccct gcaaacgctc atacgccacg tcgggcacgt tcacgttgag cagcaccgcc
     1681 tgcgcgagcg ggctcgagct gaaacgctgc accagttcga cggcgacccg cgccgccgtc
     1741 tcgtagtgat caccgccctc cgcgacgagc gagatggcga gagacggtat gcccagcagg
     1801 aatccctcag tggccgcagc gacggttccc gaatagatgg tgtcgtcacc catattggcg
     1861 ccatgattga ttcccgatat gaccacgtcg ggaagcgcct cgagcagccc tgtcacagcg
     1921 aggtggacgc agtcggtcgg cgtaccgttc acataataaa acccgttctg cgatcgtcgc
     1981 acggtgagcg gacgatccag cgtaagagag ttgctggcgc cgctgcgatc gcgctcgggc
     2041 gctaccaccg tcacctccgc tacatccgtg agggcctcct gcagcatggc gatacccggc
     2101 gcgaaatagc catcgtcgtt gctgatgagg atgcgcatgg atcagcggaa gaacccggtc
     2161 atcgcggggc ggcgcggggt gatcgcaagc gtcgtaagca gcgattatcc ttcgctggct
     2221 gagatggtta aaacacgctg gattgtatca tgccccagcg gtctgcccgg gcgctgcgac
     2281 ctcggtttcc tcaatgcgcc tcaggcggat aacgcaaagg agcgatcgtg ctcgagcgac
     2341 ggaatcatgc cgcgcacgcg agcgacacgc gcaggctcga tgtcggcgta caccacgccg
     2401 acatcctccc cggcctcggc gagaatttcg ccccacggat cgacgatgag cgaatgcccg
     2461 taggtctgcc tcctgcccgg atgatcgcca cacatcgcgg gtgcgaggat gtagcaaccg
     2521 ttttcgatcg cacgggcacg cagcaatgca tgccagtgcg cacttccggt ctgccgctgg
     2581 aacgaggacg gcacggtgat gaactgcgca ccggcttgcg ccagcgaacg gaagagcgcg
     2641 gggaagcgca ggtcatagca cactgtcagc ccgatacgtc cccacgggat gtccgcgatg
     2701 atggcgtgcg cgcccggccg gtacgccgaa gattcgcgga tcaccgtccc gtcgggaagc
     2761 gttgcatcga acatgtgtat cttgtcgtaa gtcgcaacga cgccgccgtc ggacgacagc
     2821 agatacgagc ggttcgcaat gcgcgcttcg tccgtccgta cggtgagcga tcccacgagc
     2881 agccactttc cgagctcggc ggcgagcgcc tgcagggcgg cgagcgcctc cccgccctcc
     2941 gcatcaaacg cacgctcgcg catcacacgg ccgctgccgt cgagcagcag cgcatattcg
     3001 ggcaggagca gaaagtccgc acccgcggcg gcggcgcgcg ccagccttcg caccgtttcg
     3061 agattggcct gcgggtcatt tcccgaattg agctgaatgc atgccgcccg aaatgccttt
     3121 gcgtcgctcg cgctcaatgc gcctccacgg ggtcgatcga agcttcgcat agtacacgca
     3181 ggcgacgccc gggtgaaccg acgtgcggtt cgtgcgtcgg gaatcagctg acggtcgccg
     3241 gtgtcgcgat cgacctgtgc gg
//
LOCUS       DASURG010000064         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_831378, whole genome shotgun sequence.
ACCESSION   DASURG010000064 DASURG010000000
VERSION     DASURG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831378"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFY80_01105"
     CDS             <1..1247
                     /locus_tag="VFY80_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6006527.1"
                     /translation="AAMAETLVDALRSRVPDVEFIPVGSLRRAVETCGDLDILAIGAR
                     PEVMDTFVSHPLVERVLGRGETKSSVLLRGGYQADLRLVPAESRGAAMQYFTGSKAHN
                     IALRDRALDRGLRLNEYGLFRVADNVSLAGEAEEGIYEALELAWIPPELREYRGEIAA
                     AAEGRLPALITHGDLRGDLHMHTTETDGKDELEVMARAARAAGLEYIAVTDHSKALAM
                     ANGLDEKRALEHAARIRALDGQIDGLRVLAGIECDILPDGSLDLSDECLSALDIVIAS
                     VHSAMRQEEAEMTARIIRAIEHPAVDIIAHPTNRILLRREPVKVNLERVLDAAARHGV
                     ALEINCQTDRLDLSDVNARLARDRGVKLVISSDAHSTSALSWTRWGTLVARRAWLTPA
                     DVLNTLPFEEFTRALRRNRARV"
     gene            1251..1442
                     /locus_tag="VFY80_01110"
     CDS             1251..1442
                     /locus_tag="VFY80_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006528.1"
                     /translation="MDPLAEIKKLYFSTTPKTIARDFARAIDLLKQMPEEERERAAVF
                     MEGLAELRKEWKGGRRRDE"
     gene            complement(1449..>2155)
                     /locus_tag="VFY80_01115"
     CDS             complement(1449..>2155)
                     /locus_tag="VFY80_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006529.1"
                     /translation="LLAARGAIAPRALEQLALLALLTEDADPEVRRTAAETLDRIPTE
                     AISTFIARSEVPAELRAFFVARGVPVASAPAAEAPEAFVDEDTVDYGPEPVTEEEKQS
                     IVQRLATMSVPERVKAAMKGGREMRAILIRDPNKLVALAVLSSPKMSETEVESIARMG
                     SVSEDVLRAIAQNRAWTKNYGIILALVRNPKTPLTFSMNFLSRLVDKDLKQLSTNRNV
                     PETLRIAARKKVVLSG"
BASE COUNT          352 a          736 c          713 g          354 t
ORIGIN      
        1 ccgcggcaat ggccgagacg ctcgtcgacg cgcttcggag ccgcgtgccc gacgtcgagt
       61 tcattcccgt cggcagcctg cgccgggccg tcgaaacctg cggcgacctc gacatcctcg
      121 ccattggcgc gcggccggag gtgatggaca cgttcgtttc gcatccgctc gtcgagcgtg
      181 tgctcggacg cggcgaaacg aagtcgagcg ttctgctgcg cggcggctac caggcggatc
      241 tgcgcctggt tccggccgag agccgcggcg ccgccatgca gtacttcacc ggctcgaagg
      301 cccacaacat cgcgctgcgc gatcgcgcgc tcgaccgcgg tctgcgcctc aacgagtacg
      361 ggctcttccg cgtcgcggac aacgtgagcc tcgcgggcga ggccgaagaa ggcatctatg
      421 aggcgttgga gctcgcctgg atcccgcccg agctgcgcga gtaccgcggc gagattgccg
      481 cggccgcgga aggacggctt ccagcgctca tcacacacgg cgatctgcgc ggcgacctgc
      541 acatgcacac gaccgagacc gacggcaagg acgagctcga ggtcatggca cgagccgcgc
      601 gcgctgcagg tctcgagtac atcgcggtga ccgatcacag caaggcgctg gcgatggcca
      661 acgggctcga cgagaagcgc gcgctcgagc atgccgcccg catccgcgcg ctcgacggcc
      721 agattgacgg cctgcgcgtg ctcgcgggaa tcgaatgcga catcctgccc gacggctcgc
      781 tggacctctc cgacgaatgc ctctcggccc tcgacatcgt cattgcctcg gtgcattcag
      841 cgatgcggca ggaagaggcg gagatgaccg cgcgcatcat ccgcgccatc gagcatcccg
      901 cggtcgatat catcgcgcat ccgaccaatc gcatcctgct gcggcgcgag ccggtgaagg
      961 tgaacctgga gcgcgtcctc gatgccgccg cgcgtcacgg cgtcgcgctc gaaatcaact
     1021 gtcagaccga tcggctcgat ctctccgacg tgaacgcccg gctggcgcgc gaccgcggcg
     1081 tcaagctcgt gatctcgagc gatgcccact cgacgagcgc gctctcgtgg acgcgctggg
     1141 ggacgctcgt tgcgcgccgc gcctggctca cgccggccga cgtgctcaac acgctgccct
     1201 tcgaggagtt cacccgagcc ttgcggcgga accgcgcccg ggtgtaggcc atggatcccc
     1261 tggcggagat caagaagctg tacttctcca ccacaccgaa aacgattgcg cgggatttcg
     1321 cgcgcgcgat cgacctgttg aaacagatgc ccgaagagga gcgtgagcgc gcggcggttt
     1381 tcatggaagg gctcgccgag ctgcggaagg aatggaaggg cggccgccgg cgcgacgagt
     1441 gaaacgactc agccggacag caccactttc ttccgcgcgg caatgcgcag cgtctcgggc
     1501 acgttgcggt tggtcgacag ctgcttcaga tccttgtcga ccagccggct caggaaattc
     1561 atcgagaacg tcagcggcgt cttgggattt cgcaccagcg ccaggatgat cccgtagttc
     1621 ttggtccagg cgcgattctg cgcgattgcc ctcagcacgt cctcgctcac gctgcccatc
     1681 cgcgcgatgg attcgacttc ggtttcggac atcttggggc tgctcagaac ggccagcgcc
     1741 acgagcttgt tcggatcgcg gatgaggatg gcgcgcatct cgcgcccgcc tttcatcgcc
     1801 gccttgacgc gctccggcac cgacatcgtt gcaagccgct gcacgatgga ttgcttctct
     1861 tcctccgtga ccggctcggg tccgtaatcg accgtgtcct catcgacgaa cgcttcaggc
     1921 gcttcggcag cgggagcgct tgcgacgggc acgccgcgcg cgacgaagaa cgcgcgcagc
     1981 tcggcgggca cttcgcttct ggcgataaag gtcgagattg cctccgtcgg aattcgatcg
     2041 agcgtttccg ccgcggtgcg ccggacctcg ggatccgcgt cttcggtcag cagcgcaagc
     2101 agcgcgagct gttccagggc ccgcggcgcg atggcgcctc gcgccgcgag cagtc
//
LOCUS       DASURG010000065         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1035449, whole genome shotgun sequence.
ACCESSION   DASURG010000065 DASURG010000000
VERSION     DASURG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035449"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFY80_01120"
     CDS             complement(<1..415)
                     /locus_tag="VFY80_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6006530.1"
                     /translation="MSESGPTAAPPRQLGFWMCLALVVGNMIGSGVFLLPASLAPYGL
                     NSILGWIFTASGGVLLAIVFAQLARVYPQAGGPYLYPRLAFGECCGFVMAWGYWMSVW
                     VGNAAIAIGSVAYLAELVPAIKTTQGAPAIVAVVLI"
     gene            complement(431..>2176)
                     /locus_tag="VFY80_01125"
     CDS             complement(431..>2176)
                     /locus_tag="VFY80_01125"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX6006531.1"
                     /translation="GNVALWLLEVVGGKRTRIAARERQYARPMATLKIQLPAAGRISV
                     VGSDNRYYGPTDRWIHADDGFDPVRVPSEVRYFHCPAECEVPVPEGETYVTAWRGMEF
                     TPMSQKIDAQRDGASVVAFNLLPLKLPDWAPRSVSADLHVHMNYGGHYRNTQENLIEQ
                     ARAEDLDVVYNLVVNKEQRIPDIASVIPAQTAIPAQAVIPAQAVIPAKGVIPAKAGTQ
                     MDSRLRGNDGSQMDSRLRGNDGSQMDSRLRGNDGIRLFTNQEYHTSFWGHLGLLHTQR
                     YLTPDFSAYQFSALSSPYPTNGVIADLTHEQGGLVGYVHPFDWFPEPERESLTNALPA
                     DVALGKVDYMEVVGFADHKATAAVWYKLLNLGFRVPTGAGTDAMANYASLRGPVGMNR
                     VYLATGGSVEPEALVSALEEGRTFATNGPQLALEVDGKAPGDTLTLPAGGQTVRYRAA
                     LRSPVAIDHFEVVQNGVVVARHRLGKGRTQADVSGQLKLRQSGWIVLRAWNDGADPLI
                     FDLYPYATTSPVYVSVGGAPANSPADAQYFVHWLDRTIAAAEARTDYNDERERRETLD
                     YLHAARAVYVQKAQQ"
BASE COUNT          364 a          699 c          729 g          384 t
ORIGIN      
        1 agatcagcac gaccgcaacg atcgccggcg cgccctgcgt cgtcttgatc gccggcacga
       61 gctctgccag atacgcgacg ctgccgatcg caatcgcggc gttgccgacc cacaccgaca
      121 tccagtagcc ccacgccatg acgaagccgc agcattcgcc gaatgcgagg cgcggataga
      181 gatacgggcc gcccgcttgc gggtagacgc gcgccagctg cgcgaacacg attgcgagca
      241 gcacgccgcc gctcgccgtg aaaatccagc cgaggatgct gttcaaaccg tagggagcga
      301 gcgacgccgg cagcaggaac acgcccgagc cgatcatgtt gccgacgacc agcgcgaggc
      361 acatccagaa gccgagctgg cggggcggcg ctgcggttgg gccggattcg ctcatgacat
      421 catctgggaa tcactgttgc gccttctgga cgtacacggc ccgcgccgcg tgcagatagt
      481 cgagcgtttc gcgccgctcg cgctcgtcgt tgtagtcggt ccgggcctcg gctgccgcga
      541 tcgtgcggtc gagccagtgt acgaaatatt gcgcgtcggc aggcgaattc gccggcgcgc
      601 cgccgacgct gacataaacc gggctggtgg tggcgtacgg gtagaggtcg aagatcaggg
      661 gatcggcgcc gtcgttccat gcgcgcagca cgatccagcc gctctggcgc agtttgagct
      721 gcccggacac gtccgcttgc gtgcgccctt tgccgaggcg atggcgcgcg acgacgacgc
      781 cgttctgcac tacctcgaag tgatcgatgg cgacaggcga gcgcagcgcg gcgcgataac
      841 gaacggtctg tccgccggca ggcagcgtca gtgtgtcacc gggtgctttg ccatccactt
      901 cgagcgcgag ctgcggtccg ttcgtcgcga acgtgcggcc ttcctcgagt gcagacacga
      961 gcgcttccgg ctcgacgctg ccgcccgttg cgagatatac gcgattcata ccgaccggtc
     1021 cgcgcaggct cgcatagttc gccatcgcgt cggtgcccgc gccggtgggc acgcggaagc
     1081 cgaggttcag cagcttgtac cagactgcag ccgtagcctt gtggtccgcg aagccgacga
     1141 cctccatgta atcgaccttg ccgagcgcga cgtcggccgg caaggcgttg gtcagcgact
     1201 cgcgttcggg ctcgggaaac cagtcgaacg gatgcacgta gccgaccagc ccgccctgct
     1261 cgtgcgtgag gtccgcgatc acgccgttcg tcgggtaagg cgagctcaga gcgctgaact
     1321 ggtacgcact gaagtccgga gtcaggtagc gctgcgtgtg cagcaggccg aggtgacccc
     1381 agaagctggt gtgatattcc tggttcgtga agagcctgat cccgtcattc ccgcgcaagc
     1441 gggaatccat ctggctcccg tcattcccgc gcaagcggga atccatctgg cttccgtcat
     1501 tcccgcgcaa gcgggaatcc atctgggtcc ccgctttcgc ggggatgacg cctttcgcgg
     1561 ggatgacggc ttgcgcgggg atgacggctt gcgcggggat ggcggtttgc gcggggatga
     1621 cgctcgcgat gtccggaatg cgctgctcct tgttgacgac gaggttatag accacgtcga
     1681 ggtcttcggc gcgcgcctgt tcgatcaggt tctcttgcgt gttccggtag tgaccgccgt
     1741 agttcatgtg cacgtgcaga tcggcgctga cgctgcgcgg cgcccagtcc ggaagcttca
     1801 gaggcaggag gttgaaagcg acgacgcttg cgccgtccct ttgcgcatca atcttctgac
     1861 tcatcggcgt gaattccatg ccgcgccatg cagtcacgta ggtctcgccc tccggcactg
     1921 gcacttcgca ttcggccggg cagtgaaagt aacgaacttc ggagggcact cgtacgggat
     1981 cgaagccgtc gtcagcgtgg atccagcgat cggtcggacc gtagtagcgg ttgtcggagc
     2041 cgaccacgct gatgcggccg gcagcgggga gctgaatttt cagcgtcgcc atcgggcgag
     2101 cgtactgccg ctcgcgcgct gcaatgcgcg tgcgcttgcc gccgaccacc tcgagcagcc
     2161 acagcgccac attgcc
//
LOCUS       DASURG010000066         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1342908, whole genome shotgun sequence.
ACCESSION   DASURG010000066 DASURG010000000
VERSION     DASURG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342908"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2505)
                     /gene="rpoC"
                     /locus_tag="VFY80_01130"
     CDS             complement(<1..2505)
                     /gene="rpoC"
                     /locus_tag="VFY80_01130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6006532.1"
                     /translation="MKALLDLFKQVTQEEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVIDPGMTPLKRCQLM
                     TEDDYLAKVEEFGDDFSAAMGAEGVRELLRNLDLKGEIDTLRTELAATNSDSKIKKIA
                     KRLKVLEAFNKSGIKPDWMIMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGQRVDYSGRSVIVVGPTLKLHQCGLPKLMALELFKPFIFNKLEVMGLAT
                     TIKAAKRMVESEEPIVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLSSNNILSPANGEPIIVPSQDIVL
                     GLYYITREKVGARGEGSCFINVAEASRAFDTGQVEVNARIQVRIREFEVDEVGERREK
                     VTRYETTVGRALLSEILPAGLPYELINKPLKKKEISRLINASFRRCGLRETVIFADKL
                     MYAGFTMATRAGLSIAVDDMLVPAQKNALIAEAEKEVQEIEKQYTSGLVTQGERYNKV
                     VDIWGRAGDLVAKAMMEQLGVEPVEDWDPKTGERVLRRDKDGKAVMQESFNSIYMMAD
                     SGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLAD
                     TALKTANSGYLTRRLVDVTQDLVVTEDDCGTSQGVSM"
BASE COUNT          421 a          839 c          755 g          517 t
ORIGIN      
        1 catcgatacg ccctggctcg tcccgcaatc gtcctcggtg accaccagat cctgcgtcac
       61 gtccaccaga cgccgcgtga ggtagcctga gttcgcagtc ttgagcgcgg tatcggcgag
      121 acccttgcgg gcgccgtgcg tcgagatgaa gtactgcagc acgttcaggc cttcgcgaaa
      181 gttcgccgtg atcggcgtct cgatgatcga gccgtccggt ttcgccatga gaccgcgcat
      241 gccggcgagc tgccggatct gcgcggcgga gcccctcgcg cccgaatcag ccatcatgta
      301 gatggagttg aacgattcct gcatcaccgc cttgccgtcc ttatccctgc gcagcacgcg
      361 ctcgcccgtc ttcggatccc agtcctccac cggctcgacg ccgagctgct ccatcatcgc
      421 cttggcgacg aggtctccgg cgcggcccca gatgtccacg accttgttgt agcgttcgcc
      481 ctgcgtgacc agccccgagg tgtactgctt ctcgatttcc tggacctcct tctccgcttc
      541 ggcgatcagc gcgtttttct gcgccgggac cagcatgtcg tcaaccgcga tcgacagacc
      601 ggcgcgggtc gccatggtga atccggcgta catcagcttg tcggcgaaga tcaccgtctc
      661 gcgcagcccg cagcggcgga acgaggcgtt gatgagccgc gagatctcct tcttcttgag
      721 cggcttgttg atgagttcgt acggcagtcc cgcaggcaga atttcggaca gcagcgcgcg
      781 cccgacagtc gtttcgtagc gcgtaacctt ctcgcgccgc tcgcctacct cgtccacttc
      841 gaactccctg atgcggacct ggatgcgggc attcacctcg acctgaccgg tgtcgaaagc
      901 gcgcgacgcc tccgccacgt tgatgaagca agagccctcg ccgcgcgcgc cgaccttctc
      961 gcgcgtgatg tagtagaggc cgagcacgat atcctgggag ggcacgatga tcggttcgcc
     1021 atttgccgga gacaggatgt tgttcgagga cagcatcagc gtgcgcgctt ccatctgcgc
     1081 ctccagcgag agcggcacgt gcaccgccat ctggtcgccg tcgaagtccg cgttgaacgc
     1141 cgcgcacacc agcggatgga gctggatcgc cttgccctcg atcagcaccg gctcgaaggc
     1201 ctggatgccg agccggtgca gcgtcggcgc gcggttgagc agcaccgggt gctcgcggat
     1261 cacctcttcg aggatgtccc agacgatcgg ctcctcggac tcgaccatgc gcttcgccgc
     1321 cttgatcgtt gtcgcaagcc ccatcacttc cagcttgttg aagatgaacg gcttgaagag
     1381 ctcgagcgcc atgagcttcg gcagcccgca ctggtgcagc ttcagcgtcg ggccgacgac
     1441 gatcaccgag cggcccgagt agtcgacgcg ttgtcccagc aggttctggc ggaaacggcc
     1501 gcccttgccc ttgatcatat cggcgagcga cttgagcggg cgtttgttgg cgccggtcat
     1561 cgccttgccg cgacggccgt tgtcgagcag tgaatccacc gcctcctgca gcatgcgctt
     1621 ttcgttacgc acgatgatct cgggcgcctt cagctccagc aggcgcttaa ggcggttgtt
     1681 gcgattgatc acgcgccggt agaggtcgtt gagatccgag gtcgcaaacc gcccgccgtc
     1741 gagcggcacc agcgggcgca gctccggcgg cagcaccggc agcacctcca tgatcatcca
     1801 gtcgggtttg atgcccgact tgttgaatgc ctccagcact ttgaggcgct tcgcaatttt
     1861 tttgatcttt gaatcggaat tcgtcgccgc cagctcggtg cgcagggtat cgatctcacc
     1921 cttgagatcg agattgcgca gcagctcgcg cacgccctcg gcgcccatcg ccgctgaaaa
     1981 gtcgtcgccg aactcctcga ccttggcgag atagtcgtcc tcggtcatga gctggcagcg
     2041 cttgagcggc gtcattccgg ggtcgatgac gacataggcc tcgaaataca gcacgcgctc
     2101 gatatcacgc agcgtcatgt cgagcaccat gcccagccgc gagggcagcg acttcagaaa
     2161 ccagatgtgc gcgacggggc tcgcaagctc gatgtggccc atgcgctcgc gccgcacttt
     2221 cgacagcgtc acttccacgc cgcacttctc gcagatgacg ccgcggtgct tcagccgctt
     2281 gtacttgccg cacaggcatt cgtagtcctt gaccggcccg aagatcttgg cgcagaacag
     2341 gccatcgcgc tccggcttga aggtgcggta gttgatcgtc tccggcttct taacctcgcc
     2401 gtacgaccac gagcggatct tctcgggcga cgccagcccg atcttgatgg cgtcgaattc
     2461 ttcttcttgc gttacctgtt tgaataagtc cagcagtgct ttcatttcaa actcctgggt
     2521 ataacgcggc tt
//
LOCUS       DASURG010000067         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1343173, whole genome shotgun sequence.
ACCESSION   DASURG010000067 DASURG010000000
VERSION     DASURG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343173"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..2091
                     /locus_tag="VFY80_01135"
     CDS             13..2091
                     /locus_tag="VFY80_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX6006533.1"
                     /translation="MRPGTDIPVLMGMMWHIFKNGWEDKEFIKQRVWGFDETRKEIEK
                     WTPEEVERVSGVPGAQLERVARMFATEKPATLLWSMGQTQHTIGTANVRASCTLLLAT
                     GNVGKPGTGANIIRGHDNVQGATDIGLDTVTLPFYYGLTESAWRHWGRVWEVDYDYLL
                     SHFDSKKMMETPGIPLTRWFDAVLLPKDQVDQKDNVRAMFVQGHASNSVTRIPESMKA
                     LPKLELLVVADPHPTTWASLAMQAGRKDNTYLLPVCTQFETSGSRVASNRSLQWGELV
                     VPPSFESKDDYQVLYLMARKLGLADLMFKNIKVEGDRPVPEDILREINRGSWSTGYTG
                     QSPERLKLHMANQKDFDLVTLRAKDGPCKGDYYGLPWPCWGTPEIKHPGSPILYNADA
                     AAMDGGNGFRPRFGVERNGQTLLAEGSYLPGSEIQDGYPEFTMGVLKKLGWDKDLTPA
                     ELETIMKVAGSPDHVDTVSWSTDLSGGIQRVALKHGCSPYGNGKARTVAWNLPDPTPV
                     HREPIYTPRVDLVAQYPTLPNAKQFRLPNIGFSVQKAAVDNGIAKRFPLIVSSGRLVE
                     YEGGGEETRSNRWLAELQQEMFIEINPADAADRGIKDGSWVWVTGAENNAKAKMKALV
                     TERVGKGVTWMPFHFGGWFGGEDLRSNYPQGTDPIVVGESANTIMTYGYDPATGMQEP
                     KVSLCQIRAA"
     gene            2104..2700
                     /gene="fdh3B"
                     /locus_tag="VFY80_01140"
     CDS             2104..2700
                     /gene="fdh3B"
                     /locus_tag="VFY80_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEX6006534.1"
                     /translation="MARMKFLCDADRCIECNACVTACKNENEVPWGINRRRVVTINDG
                     NPGEKSVSMACMHCTDAPCQAVCPVNCFYTTADSVVLHSKDLCIGCGYCFYACPFGAP
                     QYPRVGNFGSRGKMDKCTYCAGGPEQDGTMAEYEKYGANRLAEGKLPLCAEMCATKSL
                     LAGDGEIVAQIYKERVLKRGYGSGSWGWKTAYRETIAS"
BASE COUNT          608 a          880 c          901 g          490 t
ORIGIN      
        1 gaatatgttc gcatgcgccc cggcaccgac atcccggtgc ttatgggcat gatgtggcac
       61 atcttcaaaa acggctggga ggacaaggag ttcatcaagc agcgcgtctg gggcttcgac
      121 gaaacgcgca aggaaatcga gaagtggacc cctgaagaag tcgagcgcgt cagcggtgtt
      181 cccggcgcgc agctcgagcg cgtcgccagg atgttcgcaa ccgagaagcc ggcgaccctg
      241 ctgtggtcca tgggccaaac gcagcacacg atcggcacag cgaatgtccg cgccagctgc
      301 actttgctgc tagccaccgg caacgtcggc aagccaggaa caggcgccaa tattatccgc
      361 ggccatgaca acgtgcaggg cgcgaccgac atcggcctcg ataccgtgac gctgccgttc
      421 tactacgggc tcaccgagag cgcgtggagg cactggggcc gcgtctggga agtcgactat
      481 gactacctgc tctctcactt cgattccaag aagatgatgg aaaccccagg aattccgctg
      541 acgcgctggt tcgatgccgt tcttctgcca aaagatcagg tggatcagaa ggacaacgtg
      601 cgggccatgt tcgtgcaagg ccatgccagc aacagcgtca cgcgcatccc ggaatcaatg
      661 aaagcgttgc cgaagctcga gttgctggtc gtcgcggatc cgcatccgac cacgtgggcc
      721 tcgcttgcga tgcaggcagg acgcaaggac aacacgtacc tgctgccggt gtgcacccag
      781 ttcgagacct ccggctcgcg cgtcgcgtcc aaccgctcct tgcagtgggg cgaactggtc
      841 gttccgccta gcttcgagtc caaggatgac tatcaggtcc tgtatctgat ggcccggaag
      901 ctcggcttag cggatctgat gttcaagaac atcaaggtgg agggcgaccg gccggtccca
      961 gaagacatcc tgcgcgaaat aaatcgcgga agctggagca ccggctacac cggccagtcg
     1021 ccggagcgat tgaagctgca catggcgaac cagaaggact tcgatctcgt caccctgcgg
     1081 gccaaggatg gtccatgcaa aggtgactac tacgggttac cctggccgtg ctggggcacg
     1141 cccgaaatca agcatcccgg ctcccccatt ctctacaatg ccgacgctgc cgccatggac
     1201 ggcggaaacg gatttcgtcc ccgctttggc gtggagcgca acggccagac gctgctcgcc
     1261 gaaggctcct acctaccggg ttcggaaatt caggatggct atccagaatt cacgatgggt
     1321 gttttgaaga agctgggctg ggacaaggac cttacgcccg ctgaacttga gaccatcatg
     1381 aaggtcgcag gcagtcctga ccatgtcgat accgtgtcat ggtcgacgga tctttccggc
     1441 ggcatccagc gcgttgcgct caagcacggc tgttcgccct acggcaacgg caaggcgcgg
     1501 acggtagcgt ggaacttgcc cgatcccacc cccgtccatc gcgaaccgat ctacacgccg
     1561 cgcgtcgacc tcgttgcgca gtacccgacg ttgcccaacg cgaaacagtt tcggttgccg
     1621 aatatcgggt tctccgtgca gaaggcagcc gtcgacaacg gcatcgcgaa gcggttcccg
     1681 ctgatcgttt cgtccgggcg actggtggag tacgagggcg gtggcgagga gacccgctcc
     1741 aacaggtggc tcgccgaact gcagcaggag atgttcatcg agatcaaccc ggcggacgcc
     1801 gccgatcgcg gcatcaagga cggaagctgg gtctgggtga cgggagcgga gaacaacgcc
     1861 aaagccaaga tgaaagctct tgtcaccgag cgtgtcggaa aaggcgtgac ctggatgccg
     1921 ttccatttcg gcggctggtt cggcggtgag gatctgcgat ccaattaccc gcaggggacc
     1981 gatccgatcg tggtcggcga aagcgccaac acgatcatga cctacggcta cgacccggca
     2041 accggcatgc aggaacccaa ggtcagcctg tgccaaatca gggcggcata aggaggcggc
     2101 accatggctc ggatgaagtt tctctgcgac gccgatcgtt gcatcgagtg caacgcttgc
     2161 gtcaccgcct gcaagaacga gaacgaggtc ccctggggca tcaaccgccg ccgtgtcgtc
     2221 accatcaatg acggcaaccc gggcgaaaag tctgtgtcga tggcgtgcat gcactgcacg
     2281 gatgcgccgt gccaggcagt gtgcccggtc aactgcttct ataccaccgc agacagcgtg
     2341 gtgctgcact caaaggatct ctgcatcggc tgcggctact gcttctacgc ctgcccattc
     2401 ggggccccgc aataccctcg cgtcggcaat ttcggttcgc ggggcaaaat ggacaaatgc
     2461 acctattgcg ccggcggccc ggagcaggat ggcaccatgg ccgagtacga gaaatacggc
     2521 gccaaccgcc tggccgaggg caaactgccg ctgtgcgcgg agatgtgcgc gaccaagtcg
     2581 ctgctggcgg gcgacggcga gatcgtcgcg cagatctaca aggagcgcgt gctcaaacgc
     2641 ggctacggct ccggctcctg gggctggaag accgcctatc gcgagaccat cgcgtcttga
     2701 tgatcgatga cgcgatgagg tcgacgcgtc acggccgacg cagacaaggg aataggagaa
     2761 agcggcatga agacgctggt cgccaagctc cgcatggtcg ctgggggcct tgcgctcctg
     2821 gcgttcgcaa ttgcggcgca acccgtgaat gcccagcagc ccaagtcaat caatcccca
//
LOCUS       DASURG010000068         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_423402, whole genome shotgun sequence.
ACCESSION   DASURG010000068 DASURG010000000
VERSION     DASURG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423402"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1343
                     /locus_tag="VFY80_01145"
     CDS             3..1343
                     /locus_tag="VFY80_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX6006535.1"
                     /translation="MQAARKAFASGEWPKLNASKRGALLRKLGDLVAEKSKFLAEVEV
                     RDNGKLYAEMSAQTAYMAQWYYYYGGLADKIEGAVIPTDKPDTFNYTRYEPLGVVAAI
                     IPWNSPLLLLAWKLAPALAAGNTVVIKPSEYTSASTLEFMKLVEQAGFPQGVVNVVTG
                     FGAEVGTPLVEHPQVAKVAFTGSDATGQRIYEAAAKGLKRVTMELGGKSPNIVFDDAH
                     LDNAVKGVISGIFAATGQTCIAGSRLLVQRSIHDQFVEKLVAFARTAKMGNPMDATTQ
                     VGPVTNPPQFEKILKYIEVAKSEGAQAVLGGARATRPECGEGWFVEPTIFTGVRNAMR
                     IAQEEVFGPVLAVIPFEDEDEAVAIGNDVVYGLAAGVWTQNMRRAFTMAERLQAGTVW
                     VNTYRAVSYLSPFGGYKRSGLGRESGQEMIKEYLQVKSVWISTATEVPNPFILR"
     gene            1443..1895
                     /locus_tag="VFY80_01150"
     CDS             1443..1895
                     /locus_tag="VFY80_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6006536.1"
                     /translation="MYFTESKRLTMAGAKKMMETAIRIAEREKFAVTVAIADAGGHLM
                     LLERMDGGRFHTVHSSTTKAACAASNKRPTTAKGAAAQPLDTSHAIGLALAAGPERWT
                     AMEGGYPVIVDGECLGGIGVSGANWELDDRIAREAVESIGAGWKIDSR"
     gene            1924..2925
                     /locus_tag="VFY80_01155"
     CDS             1924..2925
                     /locus_tag="VFY80_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6006537.1"
                     /translation="MALQPVKVAAIGMGWWSDVLADAVKRSGKLEIVACYTRSPEKRD
                     AFATKYGCRAVPSYEAILEDRSVEAIINTTPNNVHCETTIAAAKAGKHVFLDKPIANT
                     VADARKLTRACRDAGVVLALGYQRRKESHFRWVRRSIEDGSFGRLVNAEANISRDRLG
                     QFDLKSWRYTAAGMPGGVMLQIGIHYSDVLEYLMGPVKAVCGSLAQLVLPGDNPDVAS
                     LILEHENGARSTLNACYASASEYYLMNIYGKEATAYYDLHNGLRVLKRGTNRAEPVPC
                     AKNDTIVEELEDFARAVRGDGAPEMDGEKATASLAVLLAGIKSARDGRRVEIAEILA"
     gene            3023..>3456
                     /locus_tag="VFY80_01160"
     CDS             3023..>3456
                     /locus_tag="VFY80_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006538.1"
                     /translation="MAVAVAASAQEAPFPKRGAIEMTVLFPAGTSADVTARALSAGMA
                     KALGVNVNVVNRPGAGGAIGYKHVAAQKPDGYSIVWNSNSISTTYHSGQLPFDYKNFE
                     PVARALVESVVVAVRADAKWKTLKELIAEAKSRPEQITVANSG"
BASE COUNT          707 a         1043 c         1142 g          564 t
ORIGIN      
        1 cggtgcaggc ggcgcgcaag gcgtttgcct ctggagaatg gccgaagctc aatgcttcga
       61 agcgcggcgc gctgctccgc aagctcggcg atctcgtcgc cgaaaagtcg aagtttctcg
      121 cggaggtcga ggtccgcgac aacggcaagc tctacgcgga gatgagcgcg cagaccgcct
      181 acatggcgca gtggtattac tactatggcg ggctcgcgga caagatcgaa ggtgccgtca
      241 tcccgaccga caagcccgac acattcaact atacgcgcta cgagccgctg ggcgtggtgg
      301 ccgccatcat cccgtggaat tcgcctctgc tgctgctcgc ctggaaactc gcacccgcgc
      361 tcgccgccgg caacaccgta gtgatcaagc cgtccgaata cacctctgcg tccaccctcg
      421 aattcatgaa gctcgtcgag caggcgggtt ttccgcaggg tgtggtcaac gtcgtgaccg
      481 gattcggcgc cgaagtcggc acgccgctcg tcgagcatcc gcaggtcgcg aaagtggcct
      541 ttacgggctc ggacgcgacg ggtcagcgca tttacgaagc cgcggcgaaa ggattgaagc
      601 gcgtgacaat ggaactgggc gggaaatcgc ccaacatcgt gttcgacgac gcgcatctcg
      661 acaacgccgt caagggcgtc atctcgggca tcttcgcggc aacgggacag acttgcatcg
      721 caggctcacg gcttctggta cagcgctcga tccacgatca attcgtggag aaactcgtcg
      781 cgttcgcacg cacggcgaag atgggaaacc cgatggacgc gacgacgcag gtcgggccgg
      841 tcacgaatcc accgcagttc gaaaaaatcc tgaagtacat cgaagtcgcg aagagcgaag
      901 gcgcgcaggc cgtactgggt ggcgcgcgcg cgacgcgacc ggagtgcggc gaaggctggt
      961 tcgtcgagcc caccattttc accggcgtca ggaacgcgat gcgcatcgcc caggaggaag
     1021 tgttcggccc ggtgctcgcc gtcattccgt ttgaagacga agatgaagcc gtcgccatcg
     1081 gcaacgatgt ggtttacggg ctcgcagccg gcgtgtggac acagaacatg cgccgcgcct
     1141 tcaccatggc ggagcgtctg caggcgggca ccgtgtgggt caatacttac cgcgcggtga
     1201 gctatctttc gcctttcggc ggctataagc gcagcggtct cggccgcgag agcggacagg
     1261 agatgatcaa ggagtacctg caggtgaaga gcgtctggat ttccaccgcc accgaggtcc
     1321 cgaatccttt tatactgcgc tagccgtccc gtcggcgccc cgcgcttccc tgaccaacga
     1381 gcgagaattg cgttccgcct ctgcatgaat agcgcgccgg ggtgattaga agaggaaaag
     1441 acatgtactt caccgaatcg aaacgcctca caatggcggg cgcgaagaaa atgatggaga
     1501 ccgcgattcg catcgccgag agagagaagt tcgcggtcac ggttgcgatt gcggatgccg
     1561 gcggtcacct gatgctgctc gaacggatgg acggcggacg attccacact gtccattcct
     1621 cgacgaccaa ggccgcctgt gcggcatcga acaaacgccc gaccaccgcg aaaggcgctg
     1681 ccgcccaacc gctcgacacg agccacgcca tcgggcttgc gctcgccgcg ggtcccgagc
     1741 ggtggaccgc gatggaaggc ggctatcccg tcatcgtgga cggcgaatgc ctcggaggca
     1801 tcggcgtgag cggcgcgaac tgggagctcg acgaccgcat cgcacgagag gcggtggagt
     1861 cgatcggcgc gggctggaag atcgattcac gataagggaa tgacggagga ggaagcacac
     1921 gctatggcat tgcaaccggt caaagtcgcc gccatcggca tgggttggtg gtccgacgtc
     1981 ctcgccgacg cggtcaagcg ctccggcaag ctcgagatcg tcgcctgcta cacgcgatcg
     2041 ccggagaaac gagacgcctt cgcgacgaag tacggctgcc gcgcggtgcc aagctacgaa
     2101 gcgatcctcg aagaccgcag cgttgaagcg atcatcaaca cgacgcccaa caacgtgcac
     2161 tgcgagacga cgattgcggc ggcgaaggca ggcaagcacg tctttctcga caaaccgatc
     2221 gccaatacgg tcgccgatgc gcggaagctc acccgtgcgt gtcgtgacgc gggggtggtg
     2281 ctcgcgctcg gataccagcg gcggaaggag agtcacttcc gctgggtccg gcggagcatc
     2341 gaggacggct cgttcggccg gctggtcaac gcggaagcca acatcagccg cgatcgactg
     2401 gggcagttcg atctcaaatc ctggcgctac accgcggcgg gcatgccggg cggcgtcatg
     2461 ctgcagatcg gtatccatta cagcgacgtg ctcgagtacc tgatgggacc ggtcaaggcg
     2521 gtgtgcggca gtctcgcgca gctcgttctt cccggggata acccggatgt cgcaagtctc
     2581 atcctcgagc acgagaacgg cgcgcgctcg acgctcaacg catgctacgc ctccgcgtcc
     2641 gagtactacc tgatgaacat ctacggcaag gaagcgaccg cgtactacga tctgcacaac
     2701 ggcctgcgtg tgctcaagcg cgggacgaac cgcgccgagc ccgtgccgtg cgcgaaaaac
     2761 gacaccatcg tcgaggagct cgaggacttc gcgcgggccg ttcgcggcga cggagcgccg
     2821 gaaatggatg gcgaaaaggc gaccgcgtcc cttgccgtgc tgctcgcagg tatcaaatcg
     2881 gcgcgtgatg gacggcgcgt ggagatcgcg gaaatcctgg catgacattt cccctgaaaa
     2941 tgacagcgta gtgaatacga cgaggaggag agcgatgaaa ggatggaggc attttgttcg
     3001 ggtagcggga tcgatagcgt gcatggccgt cgccgtcgcg gcgagcgcac aggaggcgcc
     3061 atttccgaag agaggcgcga tcgagatgac ggtgctcttt cccgccggca cttcggccga
     3121 cgtgacagcg cgcgcgctct ctgccggcat ggcgaaagcg ctcggcgtca atgtgaacgt
     3181 cgtcaatcgt cccggtgcgg ggggcgcgat cggctacaag catgtcgccg cgcagaagcc
     3241 ggacggctat tcgatcgtgt ggaactcgaa ttcgatatcg accacttatc actccgggca
     3301 gctgcctttc gactacaaga atttcgagcc ggtcgcacga gcgctggtgg agtcggtggt
     3361 ggttgccgtg cgggccgatg ccaaatggaa gacgctgaag gagctcatcg ccgaggcaaa
     3421 gtcgcgcccc gaacagatca cggtggcgaa ttccgg
//
LOCUS       DASURG010000069         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1343321, whole genome shotgun sequence.
ACCESSION   DASURG010000069 DASURG010000000
VERSION     DASURG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343321"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1424
                     /gene="glmM"
                     /locus_tag="VFY80_01165"
     CDS             78..1424
                     /gene="glmM"
                     /locus_tag="VFY80_01165"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6006539.1"
                     /translation="MKRKYFGTDGVRGKVGVAPITPEVVMHLGYAAGKVLVARNPGSE
                     RPAVLIGKDTRISGYMLESALEAGLSAAGIDVLLVGPMPTPAVAYLTRALRLAAGIVI
                     SASHNPFDDNGIKFFSADGTKLPDETENAIERELAQPLACLASSALGKARRIDDAAGR
                     YIEFCKSAFPNHLDLKGIRIVLDCAHGAAYHIAPHVFHELGADVIAIGTEPDGFNIND
                     GVGTTHPRALQQAVKRNKAHLGIALDGDGDRVLMADADGVLHDGDELLYVIARHKLES
                     GELKGGIVGTQMTNLGLEQALAALGIAFARARVGDRYILEMLHERGWRLGGENSGHIV
                     CLERHTTGDGIISALQVLRALREGGMTLERAAAGVTLYPQVLINVQLTKRFNFRNHRS
                     VKSVIAKAEQDLGSSGRVLLRESGTEPVIRVMVEGKPRARVKMLARTIADAVRGAV"
     gene            1477..2238
                     /gene="tpiA"
                     /locus_tag="VFY80_01170"
     CDS             1477..2238
                     /gene="tpiA"
                     /locus_tag="VFY80_01170"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6006540.1"
                     /translation="MRGKFVAGNWKMNGSLAFNRALLEELVPAISPLGSVKRAMCAPF
                     PYLAQVGALLERSGIELGAQDVCQFDAGAYTGGVSGAMLSDVGCRYVIVGHSERRSIF
                     GESDELVALKFVAALKAGLVPILCLGESLAEREGGLTESVVARQLDAVIARAGAASLG
                     RAVIAYEPVWAIGTGRTATPEQAQAVHAFIRGRVSAGEERVAAGLTLLYGGSVKASNA
                     AQLFSMPDIDGGLIGGASLVAAEFSAICRSAAGTA"
     gene            2251..>2531
                     /gene="secG"
                     /locus_tag="VFY80_01175"
     CDS             2251..>2531
                     /gene="secG"
                     /locus_tag="VFY80_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX6006541.1"
                     /translation="MEIVILVLHVIAALAIIGLVLLQHGKGADVGAAFGSGASGSLFG
                     ATGSANFLSRATAILAVIFFATSLGLTLFSTRKGEDKGVMATQPAPKPAA"
BASE COUNT          450 a          756 c          882 g          443 t
ORIGIN      
        1 cggggattcc gacggcggaa aggctcgttg ctgggcgagc acttggcgcg agcgtggcgt
       61 gacggcagaa tgagaccatg aaaagaaaat acttcggcac cgacggcgtg cgcggcaagg
      121 tgggcgttgc accgatcacg ccagaagtcg tgatgcacct gggttatgcg gccggcaagg
      181 tgctcgttgc acgaaatccc ggctcggaac gtcccgccgt actgattggg aaggacacgc
      241 ggatatcagg ctacatgctg gagtccgcgc tcgaggccgg gctctccgcg gcgggtatcg
      301 acgttttgct ggtgggaccg atgccgacgc ccgcagtcgc ctatctcacc cgcgcgctgc
      361 gcctcgcggc agggatcgtg atcagcgctt cgcacaaccc gttcgacgat aacggcatca
      421 aattcttctc cgcggacggc accaagctcc cggacgaaac ggaaaacgcc atcgagcgcg
      481 agctcgcgca gccgctcgcg tgcctagcgt catcggctct cggcaaggca cgccgtatcg
      541 acgatgcggc cgggcgctac atcgagttct gcaagagcgc gtttcccaac cacctcgatc
      601 tcaaggggat tcggatcgtg ctcgactgcg cgcacggagc ggcgtatcac atcgcgccgc
      661 acgtgtttca tgagctgggc gccgacgtca ttgcgatcgg cacggagccg gacgggttca
      721 acatcaacga cggggtgggt acgacgcacc cgcgcgcgct gcagcaggcc gtgaagcgca
      781 acaaggcaca tctcggcatt gcactggatg gtgacggtga ccgcgtgctg atggccgatg
      841 ccgacggcgt gctgcacgac ggcgacgaac tgctctacgt gatcgcccgc cacaagctcg
      901 agagtggcga actgaagggc ggaatcgtcg gcacacagat gaccaacctg ggcctcgaac
      961 aagcgctcgc cgcgctgggc atcgcctttg cgcgggcgcg cgtgggagac cgctacatcc
     1021 tcgagatgct gcacgagcgt ggatggcgtc tcgggggcga gaattcaggg catatcgtct
     1081 gcctcgagcg gcacacgacg ggcgacggca tcatctcggc gctgcaggtg ctgcgcgcgc
     1141 tgcgcgaggg cgggatgaca ctcgaacgcg cggctgccgg tgtcacgctg tatccccagg
     1201 tgctcatcaa cgttcagctt acgaagcgtt tcaatttcag gaatcaccgc tcggtgaaat
     1261 cggttatcgc caaagcagaa caggacctcg gatcgtcggg tcgcgtgctg ctgcgcgagt
     1321 cgggaaccga gcccgtcatt cgcgtcatgg tggaaggcaa gccgcgggcg cgcgtgaaga
     1381 tgctcgcgcg gacgatcgcg gacgcggtgc gcggcgcggt ttgatcgccc cttcgcgctg
     1441 cgataaaatt cgacgatttc gcgagccgga tgggcgatgc gcggcaagtt cgtagcgggc
     1501 aactggaaga tgaacggcag cctcgctttc aaccgggcgc tgctcgagga gcttgtcccg
     1561 gcgatatcgc ctctcggcag cgtcaaacgc gcaatgtgtg cgccgtttcc gtatctcgcg
     1621 caggtggggg cgctgctgga acgcagtggc atcgaactcg gcgcacagga tgtctgtcag
     1681 ttcgatgcgg gtgcctacac cgggggtgtt tccggggcga tgttgagcga tgtcggatgc
     1741 cgctacgtga tcgtggggca ttcggagcga cgctcgattt tcggcgagtc ggacgaactc
     1801 gtcgcgctga aattcgttgc ggcgctgaaa gcaggactcg ttccgatcct gtgcctgggc
     1861 gaatcgctgg cggagcgcga gggcgggctc accgagtcgg tcgtcgcccg gcaacttgat
     1921 gcggtcatcg cacgtgccgg cgcagcaagt ttggggcggg ccgtgatcgc gtatgaaccg
     1981 gtgtgggcca tcggtacggg cagaacggcg acgcccgagc aggcgcaggc ggtgcacgcg
     2041 ttcatacgcg gtcgggtttc ggccggggaa gagcgagtgg cagcgggcct taccctgctt
     2101 tatggcggga gtgtcaaggc ttcgaacgcg gcgcaattgt tcagcatgcc cgacatcgac
     2161 ggcggtctca tcggcggcgc atcgctggtg gctgcggaat tcagtgcaat ctgccgctcc
     2221 gcagccggaa cggcatgaca tgagagtcag atggaaatag tcatactcgt cttacacgtg
     2281 atcgcggcgc tcgccatcat cgggctcgtg ctcctgcagc acgggaaggg cgccgatgtc
     2341 ggcgcggcct tcggcagcgg cgcctccggt agtctcttcg gggcgaccgg ctcggcaaac
     2401 tttctgagcc gggcgacggc gatacttgcc gtcattttct ttgcgacgag tctgggactg
     2461 acgctgtttt cgacccgaaa aggcgaggac aagggcgtca tggccacgca gccggcgcca
     2521 aagccggcag c
//
LOCUS       DASURG010000070         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_729905, whole genome shotgun sequence.
ACCESSION   DASURG010000070 DASURG010000000
VERSION     DASURG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729905"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFY80_01180"
     CDS             complement(<1..580)
                     /locus_tag="VFY80_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6006542.1"
                     /translation="MSASGGYARLFNPRGIAVVGASADPRRIGADPVRILQDTGYRGT
                     IYPVNPKYRELRELHCYPDVAALPAPCDLAIVAVNAAAVPAVLRESGRAGIPYAIVFS
                     AGFREAGASGAALEQELKSAARDAGVRVVGPNCIGMMNLNDRVYCGFGAGFANPHLKS
                     GPVALVSQSGGFAFSVVGLADAEGIGFNYIVSA"
     gene            complement(577..1542)
                     /locus_tag="VFY80_01185"
     CDS             complement(577..1542)
                     /locus_tag="VFY80_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006543.1"
                     /translation="MKAAAAVLLAVSAMLATGHATAQSYPARTVRVIVPYAAGGNTDI
                     TARAIGGKLAEVFNQQVIVDNRPGGATNIGSELAAKAAPDGYTLFMGGASNAINMSLY
                     ARPPYDTLRDFAPITLCVKGANVLSIHPSLPVKNVKELITLAKSRPGQLNFASSGLGS
                     SNQMAGELLKVMAGINIVHVPYKGNAPALTDTVAGHVHMIFSGVPALVPHIQSGRLRA
                     VAIGSLKRFQAIPEVPTFDESGVKGYEATTWFGLMAPVRTPKDIVARLNSEVTKIIAS
                     PEITKRFINDGLEPIGNSQAEFAQFIRDEIAKYAKVIKAANIKPL"
     gene            complement(1564..>2386)
                     /locus_tag="VFY80_01190"
     CDS             complement(1564..>2386)
                     /locus_tag="VFY80_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6006544.1"
                     /translation="YPDYSRQFFSAARKAPNLKWLHVFNAGVDHPIYSEMLGRGVRLT
                     TSSGSTAAAIAQTAIAGLLLLARNFPHWLAAQRRREWRPMEGRDVPRDLEGQTVLVLG
                     LGHIGKAFAHIARALGLHVIGVRRSTRQPDDPVNELHAPDRFSELLPRAQWLVVACPL
                     TPETRGLIDGEALARLPRGARLINIARGEIVNEPALITALQSGHLAGAYLDVFEKEPL
                     PPESPLWDMPSVFISPHNSAAADGNNRRVYEIFRDNLVRWMRGAPLVNEVRRAAN"
BASE COUNT          407 a          751 c          795 g          433 t
ORIGIN      
        1 cggcggagac gatgtagttg aagcctatgc cttccgcgtc cgcgagcccg accacgctga
       61 aggcgaagcc gccgctctgc gaaacgagcg caacggggcc ggacttgaga tggggatttg
      121 cgaagcccgc gccgaagccg cagtagacgc ggtcgttgag gttcatcatg ccgatgcaat
      181 tggggccgac cacgcgcact ccggcatcgc gcgccgccga cttcagctcc tgctccagcg
      241 ctgcaccgga ggcgccggcc tcgcgaaatc ctgcggagaa gacgatggca tagggtatgc
      301 cggcccgccc ggactcgcgc aggacggcag gcacggcggc cgcgttgacc gcgacgatcg
      361 cgagatcgca cggcgccggc agtgctgcaa catcgggata gcagtgcagt tcgcgcaatt
      421 cccgatattt cgggttcacg ggatagatcg tgccgcgata cccggtgtcc tggaggattc
      481 gcaccgggtc ggcgccgatg cgccgcggat cggccgaggc gccaacgacc gcaatgccgc
      541 gcggattgaa aaggcgcgcg tatccgccgc tcgcgctcat agcggcttga tattcgccgc
      601 cttgatgact ttggcgtact tggcgatctc gtcgcggatg aactgcgcga attccgcctg
      661 actgttgccg atgggctcca ggccgtcgtt gatgaagcgc ttcgtgattt cggggctggc
      721 gatgattttc gtgacttcgc tgttgaggcg ggccacgatg tccttcggtg tcctgaccgg
      781 cgccatcaac ccgaaccagg tggtggcctc gtagcctttt acccccgact cgtcgaaggt
      841 cggcacttcg gggattgcct ggaaccgctt gaggctgccg atggcaacgg cacgcaagcg
      901 tcccgactgg atgtgcggca ccagcgccgg cacgccgctg aaaatcatgt gcacgtgccc
      961 ggcgacggtg tccgtgagcg ccggcgcgtt gcctttgtag ggtacatgca cgatattgat
     1021 gcccgccatc accttgagca gttcgcccgc catctggttg gagctgccga gtccgctcga
     1081 ggcgaaattg agctggccgg gccggctttt tgcgagcgtg atcagctcct tgacgttctt
     1141 taccgggagc gagggatgga tcgaaagcac gtttgcgccc ttcacgcaca aggtgatcgg
     1201 cgcgaagtcg cgcagcgtgt cgtacggcgg ccgcgcatag agcgacatgt tgatcgcgtt
     1261 ggaagctccg cccatgaaca gcgtatagcc gtcgggcgct gccttggcgg ccagttcgga
     1321 gccgatgttg gtcgcgccgc ccgggcggtt gtccacgatc acctgctggt tgaacacttc
     1381 ggccagtttg ccgccgatcg cgcgagcggt gatatcggtg ttgccgcccg ccgcatacgg
     1441 aacgatgact cgcacggtgc gcgccgggta gctctgcgcc gttgcatgac cggtcgcgag
     1501 catagcggaa acggcgagta gcacggctgc tgcggctttc atttcaatac tcctgtcgat
     1561 gtttcagttc gcggcgcgcc tcacttcgtt caccagcgga gcgccccgca tccagcgaac
     1621 gagattgtcg cggaagatct cataaacccg gcggttattg ccgtcggccg ccgcggagtt
     1681 gtgcggcgag atgaaaacgc tcggcatgtc ccacagcggc gactcgggcg gcaggggctc
     1741 tttttcgaat acatccagat acgcaccggc aagatggccg ctctggagag cggtaatcaa
     1801 cgccggctcg ttcacgattt cgccgcgtgc gatgttgatg agtcgggcac cgcgtggcag
     1861 gcgggcgagc gcctcgccgt cgatcagccc gcgtgtttcg ggcgtgagcg gacaggcgac
     1921 gaccagccac tgcgcccgcg gcagcagttc agaaaacctg tccggtgcgt gcagttcgtt
     1981 caccgggtcg tcgggctgtc gcgtggaacg gcgcacgccg atcacgtgca gacccagcgc
     2041 ccttgcgatg tgcgcgaacg cttttccgat atggcccagt cccaggacga gcaccgtctg
     2101 gccttcgaga tcccgcggca catcgcggcc ttccatgggc cgccactcgc gcctccgctg
     2161 cgcggccaac cagtggggaa aattgcgcgc gagcagcaac aaacccgcga tcgcggtctg
     2221 cgcgatggct gccgcggtgg aaccggaaga agtcgtcaac cgcacgccgc gcccgagcat
     2281 ctcgctgtag atcggatgat ccacgccggc gttgaagaca tgcagccact tcagattcgg
     2341 cgccttgcgt gccgcagaga aaaactggcg cgagtagtcg ggatag
//
LOCUS       DASURG010000071         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_16186, whole genome shotgun sequence.
ACCESSION   DASURG010000071 DASURG010000000
VERSION     DASURG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16186"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /gene="lepB"
                     /locus_tag="VFY80_01195"
     CDS             <1..455
                     /gene="lepB"
                     /locus_tag="VFY80_01195"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6006545.1"
                     /translation="PSLDYIKRVVGLPGDRVAYRNKRLWINGEEVKVRPAGEYNYIES
                     GLSFISALKFEEVLGGHEHAILIQPEAPSVQLGGVKSFPSRDNCTYNDSGLTCTVPAG
                     HYFLMGDNRDSSSDSRYWGFVPEENIVGKAFMIWWNFDDFTRIGRSIN"
     gene            473..826
                     /locus_tag="VFY80_01200"
     CDS             473..826
                     /locus_tag="VFY80_01200"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX6006546.1"
                     /translation="MRGQRGLTLTGFMVTAVILEILALLGFKIMPAYMEFYTIQRQIR
                     IVANEVGAADRRAIENAFDRRADVENITAITGKDLEITKDGDGVIITAEYAVKVPLVG
                     NLSAYMDFKASSDKK"
     gene            854..1525
                     /gene="rnc"
                     /locus_tag="VFY80_01205"
     CDS             854..1525
                     /gene="rnc"
                     /locus_tag="VFY80_01205"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX6006547.1"
                     /translation="MDEKEFGLLIGHSFRDPQLLRRALTHRSYGSLHNERLEFLGDSV
                     LNCVIAVELFERFPKLTEGELSRLRASLVRQQTLYGVAQRFRFGEQMRLGEGEIRSGG
                     GQRPSILADAVEAVIGAAFLDGGFDASAKLVRVLFDEELDNAHPTRVGKDPKTLLQEY
                     LQSRRLGLPHYGVVATRGRADQHVFEVECVIPELNIRSMGEGTSRRSAEQDAARRAYE
                     LAMRE"
     gene            1522..2418
                     /gene="era"
                     /locus_tag="VFY80_01210"
     CDS             1522..2418
                     /gene="era"
                     /locus_tag="VFY80_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6006548.1"
                     /translation="MSASRHRAGYVAIVGRPNAGKSTLLNALVGQKVSITSRKAQTTR
                     RRIVGILTREDVQVVFVDTPGFPARRDTALNRLMNRSVIAGLHDVNAVLLVLDGMKAD
                     RREVELRKAVPAAVPVVMAINKIDRIRNKSELLPFMRKIAQQLDCSRIVPVSASRGMG
                     LDELVSVTAALMPEGPALYADDEITRSSERFLAAELIREKVFRTMGDELPYATAVDIA
                     RFETRGGVRRIYAEILVDKESQKPILIGRNGEKLKAIASRSREDMERLFGGKVFLDVW
                     VKVRRGWADDARALRRLGYEEQ"
     gene            2443..3171
                     /gene="recO"
                     /locus_tag="VFY80_01215"
     CDS             2443..3171
                     /gene="recO"
                     /locus_tag="VFY80_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6006549.1"
                     /translation="MRVDRAGHEGGPAFVLHSYPFRETSLVLEVFTRDSGRIGLVARG
                     ARRPRSALRGVLRAFQPLLLTWSGKGELRTLHKAEWHGAYAPLRGLALICGFYLNELL
                     LKFLARDDAHERLYEVYAETLNALASEADSAPTLRRFEKHLLRELGYGLILDREAESG
                     APIAAEERYTYVVDRGPLAWRGDEAGNGVELTGQTLIDMQSDTYTTMATLQQSKALMR
                     ALINHYLGDRTLHTRQLLRDLRAL"
     gene            3171..>3650
                     /locus_tag="VFY80_01220"
     CDS             3171..>3650
                     /locus_tag="VFY80_01220"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX6006550.1"
                     /translation="MLLGVNIDHVATLRQARGTRFPSPLEAALVAETAGADYITLHLR
                     EDRRHIQERDVIVLRETLKTRMNLESAVTDAMTDFALRVRPHDVCLVPEKREELTTEG
                     GLDAAGQVDRITKAVRILSATGIRVSLFIDPESRQIDAARACGAPVIELHTGKYADAP
                     "
BASE COUNT          729 a         1082 c         1184 g          655 t
ORIGIN      
        1 atccgtcact cgactacatc aagcgcgtgg tcggtctccc tggcgaccgg gtggcctacc
       61 gcaacaagcg actctggatc aacggcgagg aagtaaaagt acggcctgcg ggcgagtaca
      121 actacatcga gtcggggctc agctttatct ccgcgctgaa gttcgaggag gtgctgggcg
      181 ggcacgagca cgccatcctg atccagccgg aggcgccatc ggtccagctt ggcggcgtga
      241 agtcgtttcc ttcacgtgac aattgcacat acaatgacag cggtttgacg tgcaccgtgc
      301 ccgccgggca ctattttctg atgggcgaca atcgtgacag cagcagtgac agccgctatt
      361 ggggcttcgt cccggaagag aacatcgtag gcaaagcgtt catgatctgg tggaatttcg
      421 acgatttcac gaggataggg cggtcgataa actgaaaaag gggaggcgca tcatgcgcgg
      481 tcaacgcggt ttgacgctga cgggtttcat ggtcacggct gtaattctgg agatcctggc
      541 cttgctcggc ttcaagatca tgccggcgta catggagttc tacacaattc agaggcagat
      601 ccggatcgtt gcgaacgagg tgggcgctgc cgaccggcgg gcgatcgaga acgcgttcga
      661 tcgccgcgcc gatgtcgaaa atattaccgc catcaccggg aaggacctcg agatcaccaa
      721 ggacggtgac ggggtgatca tcacggcgga atacgcggta aaagtgccgc tggtaggcaa
      781 tctcagcgcc tacatggact tcaaagcgag ttcggacaag aagtagccgc agagacatgc
      841 ggttgcttgc aagatggatg aaaaggaatt cggcctcctg atcgggcaca gttttcgcga
      901 tcctcaactg ctgcggcggg cgctcacgca ccgcagctac ggttccctgc acaacgagcg
      961 tctcgagttc ctcggcgata gcgtgctcaa ctgcgtgatc gcagtagagc tcttcgagcg
     1021 attcccgaag ttgaccgaag gcgagctctc gcggcttcgc gcaagcctgg tcaggcagca
     1081 gacgctctat ggtgtcgccc agcgctttcg gttcggcgag cagatgcggc ttggcgaggg
     1141 tgaaatccgc agcgggggcg gacagaggcc ctcgatactc gcggacgcag tcgaggccgt
     1201 gatcggtgcc gcgttcctcg acggcggatt cgatgcatcg gcaaagctgg tacgcgtgct
     1261 gttcgacgag gagcttgaca acgcgcatcc tacccgcgtc ggcaaggatc ccaagacgct
     1321 ccttcaagag tatctccaga gccggcgcct cggtctgccg cactacggcg tcgtggcgac
     1381 acggggacgg gccgaccagc acgtcttcga agtcgagtgt gtgataccgg aactgaacat
     1441 ccgctcgatg ggggaaggca cgagccgccg cagcgccgaa caggatgcgg cacgccgcgc
     1501 gtacgagctc gcgatgcgcg aatgagcgca tcgcgccatc gcgccgggta tgtcgcgatc
     1561 gtgggccggc ccaacgccgg gaaatccacg ctgctcaacg cgctggtcgg gcagaaagtc
     1621 agcatcacct cgcggaaggc gcagaccacg cgccgacgca tcgtcggcat acttacgcgc
     1681 gaggacgtcc aggtcgtctt tgtcgatacg ccgggttttc cggcgcgccg ggatacggct
     1741 ttgaaccggc tcatgaaccg cagcgtgatc gcggggctcc atgacgtgaa tgcggtgctg
     1801 ctcgtgctcg atggaatgaa ggccgaccgg cgtgaagtcg agttgcgcaa agcggtgccg
     1861 gccgccgtgc cagtcgttat ggccatcaac aagatcgacc gtatccgcaa caaatccgaa
     1921 ctgctcccgt tcatgcgcaa gatcgctcaa cagctggact gctcgcgcat cgtgccggtg
     1981 tcagcgtcca gaggaatggg actggacgaa ctggtcagcg tcacagcggc cctcatgccg
     2041 gaaggccccg cgctttacgc agacgacgaa atcacgaggt cgagcgagcg ctttctcgcc
     2101 gctgaactca ttcgtgaaaa agtctttcgc accatgggag acgagcttcc gtatgcaacc
     2161 gcggtggaca tcgcgcgttt cgaaacccgc ggcggcgtgc gccgcatcta tgcagaaatt
     2221 ctcgtcgaca aagagagcca gaaaccgatc ctcatcggcc gcaatgggga gaagctcaag
     2281 gccatcgcat cgcggtcacg cgaggacatg gagcgtctgt tcggcggcaa ggtctttctt
     2341 gatgtctggg tgaaagtcag gcgcggctgg gccgacgacg cacgggccct gaggcgcctc
     2401 ggctacgagg aacagtagtg cccgtgagct tatccgaggt acatgagagt tgatcgcgcc
     2461 gggcacgagg gcgggcccgc cttcgtgctg catagctatc cttttcggga aacgagcctg
     2521 gtcctcgagg tctttacacg tgactcgggc aggatcggac tggttgcccg cggggcgcgt
     2581 cggccacgct ctgcgctgcg cggcgtgttg cgtgcgttcc agcctctgct gctgacgtgg
     2641 tcgggaaaag gcgagcttcg gaccttgcac aaggccgaat ggcatggcgc gtacgcgccg
     2701 cttcgagggc tcgcactgat ctgcgggttc tatctcaacg aattgctgct caagtttctc
     2761 gcccgggatg atgctcacga gcgcctctac gaagtgtacg cggagacttt gaacgccctt
     2821 gcgagcgaag cggattcggc tccgacgctg cggcggttcg agaaacatct gctgcgcgaa
     2881 ctgggctacg gtttgatcct cgaccgggag gcggagagcg gcgcgccgat cgcggctgaa
     2941 gagcgctata cctacgtcgt cgatcgcgga cccctcgctt ggcgcggcga cgaagcagga
     3001 aacggggtag aattgaccgg gcagacgctg atcgacatgc agtcggatac ctatacgacc
     3061 atggccactc tgcagcagag caaggcgttg atgcgggcgc tgatcaacca ctatctcggc
     3121 gacaggacgc ttcacacccg ccagctgctg cgcgacctgc gcgcgctgtg atgcttctcg
     3181 gcgtcaatat cgaccacgtc gctacgctgc ggcaggcgcg cggcacacgc ttccccagcc
     3241 cgctcgaggc cgcgctggtc gcggaaacgg caggcgctga ttacatcacc ctgcacctgc
     3301 gggaagaccg ccggcatatc caggagcggg acgtcatcgt cctgcgcgaa acactgaaga
     3361 cgcggatgaa tctggaatcg gccgtcaccg atgcgatgac cgatttcgca cttcgcgtgc
     3421 gcccccatga cgtatgcctg gtcccggaga agcgcgagga gctgaccacg gaaggcggtc
     3481 tcgacgccgc cggtcaagtg gacagaataa ctaaggcggt gcgcatattg agtgcgaccg
     3541 gcattcgggt gtctttattc atcgaccctg agtcgcggca gatcgacgcc gcgcgcgcat
     3601 gcggcgcacc agttatcgaa ctgcacacgg gtaagtatgc cgacgcgcca
//
LOCUS       DASURG010000072         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_16318, whole genome shotgun sequence.
ACCESSION   DASURG010000072 DASURG010000000
VERSION     DASURG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16318"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFY80_01225"
     CDS             <1..544
                     /locus_tag="VFY80_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006551.1"
                     /translation="ALAKTQPGAILMASAGIGSNTHLTGELFQVVTGVKLTHVPYKGA
                     GPAAIDLVAGHAQCIFDQVTSSGRYVKAGKLRALAVTSDKRSMQFPDVPTMSEAGAPG
                     VESTAFTGIFLPAATPRDIVTRVHGALLKVLDQPAVREAFNRLGAEVVKSTPDEVTRR
                     LTSEIAKWRKVQSQTGIRID"
     gene            560..847
                     /locus_tag="VFY80_01230"
     CDS             560..847
                     /locus_tag="VFY80_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006552.1"
                     /translation="MGKIFLVVPDAPLDEVAQVKPDRTLGTHGIRLGVLDNSKGNADH
                     LLNFIVEGVKQEFRVDSVLVKRKPASSAGATPQILDELAKEADLVVSAMAD"
     gene            878..1096
                     /locus_tag="VFY80_01235"
     CDS             878..1096
                     /locus_tag="VFY80_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006553.1"
                     /translation="MAQLTKRGLIAAVVCSDQFMKLGTAQAKVFGVPDLPLLQIPHPL
                     GGLSMDKVRERAAAALPQLVKVIREKRT"
     gene            1224..2339
                     /locus_tag="VFY80_01240"
     CDS             1224..2339
                     /locus_tag="VFY80_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006554.1"
                     /translation="MRSLGDLLKRPGAMVECDDDPEAVFELLCEKGWSDGLPVIPPTP
                     ERVERMLSYCDRDLDKPVVKIPPRYGAATPIRVAANAVMAGCKPEHFPLVLLALEAMA
                     EEPYNLYGTQATTHPCTPLLIFNGPIARELGINCGHNVMGNYFRPNAVIGRAVRLALV
                     NIGAAIPGIGDMATHGTPAKFTYCIAENEAQSPWEPLHVELGFPKDATAVTVVAGEGP
                     HNINDHESLTPEGILTMCAGTIATTGSNNAYYRGQPVLAFGPEHAATVASGGMTKREV
                     KQWLYDHATIPLSKFSKEGIERRFRRKQADEFANAPLDAPVHMWKKPDDLIVVVTGGA
                     GKHSQYVPTFGNTRAVTRMLTRASGEIVKNIGEFKSA"
BASE COUNT          490 a          778 c          826 g          395 t
ORIGIN      
        1 cgcgctcgcg aagacgcagc ccggcgcgat tctcatggcc tccgcgggca tcggcagcaa
       61 cacacatctc acgggcgaac tctttcaggt cgtgacgggc gtcaaactga cgcacgtccc
      121 gtacaaaggc gcgggccctg cggcgatcga tctcgttgcc gggcacgcgc aatgcatctt
      181 cgaccaggtc acgtcttcgg gccgttacgt gaaggccggc aaactacgcg cgctcgccgt
      241 cacctccgac aagcgctcca tgcaatttcc ggatgttccg acgatgagcg aggcgggcgc
      301 gccgggcgtg gaatcgactg cgttcaccgg tatttttctg ccggctgcga cgccgcgcga
      361 tatcgtgacg cgggtccacg gcgcgctcct gaaggtgctc gatcagcccg cggtgcgcga
      421 ggcgttcaat cgcctcggcg cggaggtcgt gaagagcacg ccggatgaag ttacgcggcg
      481 cctcaccagc gaaatcgcga agtggcgcaa ggtgcagagc caaacgggca ttcgtatcga
      541 ctgagctgga gaagacgaaa tgggcaagat atttctggtg gtacctgatg cgccgctcga
      601 tgaagtggcg caggtcaaac ccgatcgcac cctgggcacc cacggcatac gcctcggcgt
      661 gctggacaac agcaagggca acgccgatca cctgctcaac ttcatcgtcg aaggcgtgaa
      721 gcaggaattc agggtggatt cagtgttggt caagcgcaag cccgcatcga gcgcgggcgc
      781 gacaccgcag attctcgatg agctcgcgaa ggaggcggac ctcgtcgtca gtgccatggc
      841 cgattgaggg tcctgcacgt cgtggagtgt ccacgacatg gcgcagttga cgaagcgggg
      901 cctcatcgcc gcggtggtgt gctcggacca gttcatgaag ctcggcacgg cgcaggcgaa
      961 agtgttcggc gtgcccgatc tgccgctgct gcagatcccg catccgctcg gcggactcag
     1021 catggacaag gtgcgtgaac gtgcggcggc tgcgctgccg cagctcgtca aggtgatcag
     1081 ggagaagcgg acatgagcgg cggagcaggg atcggcggaa cgcggagcgg ggaccccgtt
     1141 tgcggggatg cgacccgcga aggacgcttg ctgccgacat gagggcgaag gagatgtgtg
     1201 atggtgctaa cggaggcagc ggcatgagat cgcttggcga tctgttgaag cgccccggcg
     1261 cgatggtcga gtgcgacgac gaccccgagg ccgttttcga gcttctctgc gagaagggct
     1321 ggagcgacgg cctgcccgtc attccgccca cacccgagcg cgtcgagcgc atgctgtcgt
     1381 actgcgaccg cgatctcgac aagccggtcg tcaagattcc gccgcgctac ggcgccgcga
     1441 cgccgatccg tgtcgccgcc aacgccgtga tggcgggctg caagccggag cattttccgc
     1501 tggtcctgct tgcgctcgaa gcgatggccg aagagccgta caacctctac ggcacgcagg
     1561 cgacgacgca cccgtgcacg ccgctcctga tcttcaacgg gccgatcgcg cgcgaacttg
     1621 gcatcaactg cggccacaac gtgatgggga attacttccg tcccaacgcg gtgatcggcc
     1681 gcgccgtgcg gctcgcgctg gtcaacatcg gcgcggcaat tccgggcatc ggggacatgg
     1741 ccacacacgg aacgcccgcg aagttcacct actgcatcgc ggaaaacgaa gcgcagagcc
     1801 cgtgggagcc gttgcacgtc gagctcggat ttccgaaaga cgcaaccgcc gtgacggtgg
     1861 tggcgggaga aggcccgcac aacatcaacg accacgagag cctgacgccc gaagggatcc
     1921 ttacgatgtg cgcgggcacc atcgcaacga cgggctccaa caatgcctac tacaggggac
     1981 aacccgtcct cgcgttcggc cccgagcacg ccgcgaccgt ggcgagcggc ggcatgacca
     2041 agcgcgaagt gaagcaatgg ctgtacgacc atgcgaccat tccgctctcg aagttctcga
     2101 aggaaggcat cgagcgccgc ttccgccgca agcaggccga cgagttcgcc aacgcgccgc
     2161 tcgatgcgcc cgttcacatg tggaaaaagc cggatgatct gatcgtcgtc gtcaccggcg
     2221 gcgcgggcaa gcactcgcag tatgtgccga ccttcggcaa cacgcgtgcg gtgacacgga
     2281 tgctcacgcg tgccagtggc gaaatcgtga aaaatatcgg tgaattcaag tccgcataga
     2341 ccggtcccga aaagcggcgc tcagtgcttc ttcacccgcc gcagcacgga tgggcgatag
     2401 tgcacaaacg gcttcgcgtc tttgcgatcc cagatcgggt gcgttgcacc gaagccatag
     2461 acgatgcagt tcagcagtcg cagggggag
//
LOCUS       DASURG010000073         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_935205, whole genome shotgun sequence.
ACCESSION   DASURG010000073 DASURG010000000
VERSION     DASURG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935205"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..403)
                     /gene="nuoK"
                     /locus_tag="VFY80_01245"
     CDS             complement(98..403)
                     /gene="nuoK"
                     /locus_tag="VFY80_01245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6006555.1"
                     /translation="MITLSHYLVLGAILFAISVVGIFLNRRNVIVLLMAIELMLLAVN
                     MNFVAFSHFLDDPAGQVFVFFILTVAAAESAIGLAILVVLFRNLSTIDVEDLDRLKG"
     gene            complement(400..1020)
                     /locus_tag="VFY80_01250"
     CDS             complement(400..1020)
                     /locus_tag="VFY80_01250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6006556.1"
                     /translation="MTFQAFIFYFFAAVLVFAALRVITARNPVHAALFLVLAFFTSAG
                     IWLLLQAEFLAIALVLVYVGAVMVLFLFVVMMLDINVGRMRERFWRYLPLGGLVALLL
                     VAEMALVLVGRYFGPEGSPVPPPLPEGYSNTKELGRLLYTEYVYPFEIAAVILLVAIV
                     AAIALTYRRRKQTKYQDPARQVAVRREERVRIVPMPAERSTAEKPQ"
     gene            complement(1023..1514)
                     /gene="nuoI"
                     /locus_tag="VFY80_01255"
     CDS             complement(1023..1514)
                     /gene="nuoI"
                     /locus_tag="VFY80_01255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX6006557.1"
                     /translation="MISRIAAFFRTFLLVELVKGLTLTGRYLFARKITVLYPEEKTPQ
                     SPRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIDSEVRADGTRRTTRYDVDLTK
                     CIFCGYCEESCPVDSIVETRILEYHGEKRGDLIYTKPMLLAIGDAYEAQIAKDRAEDA
                     RYR"
     gene            complement(1511..>2332)
                     /locus_tag="VFY80_01260"
     CDS             complement(1511..>2332)
                     /locus_tag="VFY80_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX6006558.1"
                     /translation="LLTKELIRPTAANGGLFRLGPIMAIMPALAAWAVIPFGPERVLA
                     NIDAGLLYIMAITSMGVYGIIIAGWASNSKYAFLGSLRSAAQMVSYELAMGFALVCVL
                     MAAQTLNLGEIVRMQQGSYGLFNWFWIPLFPIFLVYLISGVAETNRAPFDVAEGESEI
                     VAGFHVEYSGMAFAVFFLAEYANMILVSTLASVMFLGGWLSPVPFLPDGFPWLAAKVA
                     FLLFCFLWFRATFPRYRYDQIMRLGWKVFLPLTIAWVIVLGAWMQTPLWVWWRPL"
BASE COUNT          505 a          728 c          743 g          356 t
ORIGIN      
        1 ggcacgatga ggtagatcaa ctgcattcgc gtcatgttct atggtcgcgt tgcgccgtgg
       61 gttgcggcca gtcgcccgtc tttcttccga tcctttttca tcccttcagc cggtcgaggt
      121 cctcgacgtc gatcgtgctc aagttgcgga acagcacgac cagaatggcg agcccgatgg
      181 ccgattccgc ggcggcgacc gtgaggatga agaacacgaa aacctgcccc gccgggtcgt
      241 cgagaaagtg cgagaacgcg acgaagttca tgttgacggc aagcagcatg agctcgatcg
      301 ccatcagcag cacgatcacg ttccggcggt tcaaaaagat gcccacgacg ctgatcgcga
      361 aaaggatcgc gccgagcacg aggtagtgcg agagcgtgat cactgcggct tctccgccgt
      421 cgagcgctcg gctggcatcg gcacgatccg cacccgctcc tcacgccgca cggcgacctg
      481 ccgtgccggg tcctgatact tggtctgctt gcggcggcgg taggtaagcg caatcgccgc
      541 cacgatggca accagcagga tcacggcagc gatctcgaag ggatacacat actcggtgta
      601 gagcagccgt ccgagctcct tggtgttgct gtagccctcc ggcaacggtg gcggcaccgg
      661 ggagccctcg gggccgaaat agcgcccgac cagcaccagc gccatttcgg cgaccagcag
      721 cagcgcgacc agcccgccga gcggcagata gcgccagaag cgctcgcgca tgcgcccgac
      781 gttgatatcg agcatcatca cgacgaacag gaagagcacc attaccgcac cgacgtacac
      841 cagcaccagc gcgatcgcga ggaactccgc ctgcagcagg agccagatgc ctgccgaggt
      901 gaaaaaggcg agcacgagga agagcgccgc gtgtaccggg ttgcgggcgg tgatgacccg
      961 cagcgcggca aaaacgagca ccgccgcgaa gaagtagaag atgaacgcct ggaaggtcat
     1021 ggtcagcggt agcgcgcgtc ttcggcccga tcctttgcga tctgggcctc gtaggcatcg
     1081 ccgatggcaa gcagcatcgg cttggtgtag atgagatcgc cgcgcttctc gccgtggtac
     1141 tcgaggatgc gggtctcgac gatggaatcg acgggacagg actcctcgca gtatccgcag
     1201 aaaatgcact tggtgagatc gacgtcgtag cgcgtggtgc gccgcgtacc gtccgcgcgc
     1261 acctctgaat cgatcgtgat tgcgagcgcc gggcatacgg cctcgcacag cttgcacgcg
     1321 atgcagcgct cttcgccgtt cggatagcgc cggagcgcat ggaggccgcg aaagcgcggg
     1381 ctctgcgggg tcttctcctc cggatacagc accgtgatct tgcgcgcgaa gaggtagcgc
     1441 ccggtcagcg tgagaccctt cacgagctcc accagcagaa aagtccggaa gaacgcggca
     1501 atccgggaaa tcatagcggc ctccaccaga cccagagtgg cgtctgcatc caggcgccga
     1561 gcacgatgac ccaggcgatc gtgagcggca ggaacacttt ccatccgagc cgcatgatct
     1621 gatcatagcg gtaacgcggg aaggtcgcgc ggaaccacag gaagcagaag agcaggaatg
     1681 ccactttcgc ggccagccaa ggaaatccgt ccggcaggaa cggcacggga gagagccagc
     1741 cccccaggaa catcacgctc gccagagtag acaccagaat catgttggcg tactccgcga
     1801 ggaagaacac ggcgaacgcc atccccgagt attcgacgtg aaatcccgcc acgatttcgg
     1861 attcaccctc cgcgacgtcg aacggcgcgc ggttggtctc cgcgacaccg gaaatgagat
     1921 acacgagaaa gatcgggaag agcggaatcc agaaccagtt gaagaggccg tagctcccct
     1981 gctgcatgcg cacgatttcg cccagattga gcgtttgcgc ggccatcagc acgcacacga
     2041 gcgcgaaccc catggcaagc tcatacgaga ccatctgagc tgcggagcgg agacttccga
     2101 ggaaggcgta cttggagttg gacgcccagc cggcgatgat gatgccgtag acgcccatcg
     2161 aggtgatcgc catgatgtag agcagccccg cgtcgatgtt cgcgagcacg cgctccggcc
     2221 cgaacgggat cacagcccac gcggccagcg ccggcatgat ggccatgatc ggccccaggc
     2281 ggaacaaccc accgttggcc gccgtggggc ggatcaattc cttggtcagc ag
//
LOCUS       DASURG010000074         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_832618, whole genome shotgun sequence.
ACCESSION   DASURG010000074 DASURG010000000
VERSION     DASURG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832618"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFY80_01265"
     CDS             <1..441
                     /locus_tag="VFY80_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006559.1"
                     /translation="FGLEGLEEEEMTIPKVIGMGIKSIALFYLDPERFSPRGRNGLYG
                     LYFLSGRDHFGLPSNSSEFLMINDRTPASDGSIVMDQNYWYPYNVFTLYSVRVFRWIA
                     AKAATAGYTVDPKLRFVFTDALFESVCDRHKEDLGTMRAHERFG"
     gene            438..2066
                     /locus_tag="VFY80_01270"
     CDS             438..2066
                     /locus_tag="VFY80_01270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX6006560.1"
                     /translation="MSGFPGTPASALARWFPNTVTFRDRQEAALKRLWGARSTVVLMP
                     TGTGKSLVYQLPVLASQQIGLVISPLIALMDQQARNLTDVGARTLTLSGVESRKAYED
                     LRRFDWSAGPAFLFVSPERIENDGYLEYLIHKHRARIVLTAVDEAHCISQWGHDFRPP
                     YKTIPSFLDRAFGRAHWPTVVCLTGTLNKGALDEILSDFRLGSEDVVRSGTMVRSNLK
                     LSVQAFADRDSKLAALDELLETHRGKKLIVYAHLKHNRITGTRALTDRYLAKRHRCAA
                     FDADLGTTEKDSLLRDFEAGTIDVVFATGAFGMGINIPDIRGVIHYLLPESIEQYYQE
                     VGRAGRDGQPAFGCLLYTSTNARVRRDQIKSNRRTKADVEEVWRAVCNESVNSLKTVS
                     PWSEFKGREAEYAIFQNLVRLGAVEILARGPNRLTCLEARGANGAAMLNRLSSATRAG
                     LTAAAMRKLGDDPVTTISRLFELYDEREVTLVQNPDKTLFFRARALSEEAVVALVESL
                     DAKIDHRLAEFETLVSLIEGGHDLQAVLSERFDT"
     gene            2269..3015
                     /locus_tag="VFY80_01275"
     CDS             2269..3015
                     /locus_tag="VFY80_01275"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX6006561.1"
                     /translation="MSIDIFFGRDIGNIAERRCLRRLLCHLTAVQTDAVVLANFVVGP
                     QRRQVDMVVATPKTAVVVEIKGYTHPVHGDLNGPWHLDLGDGKTRSLGPTNPYHQALD
                     CRYAVCDALSAHADLQGHIARAMVLGSLCVFPSPLATSALPASDHKVVIGGAPELEAL
                     LERPLPTAMALDTWRSFARSLQLSHGGEAEPRPASTLVESYLARFLELRRALAVPYIE
                     PLIAGEQVATSHLVGRLVDGVQLHIVGPSG"
     gene            3063..3389
                     /locus_tag="VFY80_01280"
                     /pseudo
     CDS             3063..3389
                     /locus_tag="VFY80_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          671 a         1028 c         1020 g          670 t
ORIGIN      
        1 ttcggtctgg aaggtttgga agaagaagaa atgaccatac cgaaggtgat cggcatggga
       61 atcaagagca ttgcgctctt ctatctcgat cccgaacggt tctcccctcg cggccgtaac
      121 ggattgtacg gcctttattt cctctcggga agggatcatt tcggattacc gagtaactcc
      181 tctgagttcc tgatgattaa tgatcgaacc ccggcttctg acggcagcat cgtgatggat
      241 cagaactatt ggtatcccta caacgtcttc acgctctaca gcgtgagggt gttccggtgg
      301 atcgccgcca aagcggcgac ggcggggtac acggtggatc caaaattgcg gttcgtgttt
      361 acagacgccc tctttgagag tgtctgcgac cggcacaagg aagatctcgg gaccatgcga
      421 gctcatgagc gctttggatg agcggcttcc caggaacgcc agcttccgcg ctcgcccgct
      481 ggtttcccaa tactgtgacc tttcgcgacc gccaggaggc agccctcaag cgcctgtggg
      541 gcgcgcgatc taccgtagtg ctgatgccca ccggcaccgg aaaatcgcta gtttaccagc
      601 ttcctgtctt ggcttcgcag caaatcggtc tggtgatctc accactgatt gcattgatgg
      661 accagcaggc gcggaatctt accgatgtgg gcgcacggac gctaacgctt tcaggtgtgg
      721 agagccgaaa agcctacgaa gacctgcgcc ggttcgactg gagtgcgggg ccggcctttc
      781 tgttcgtatc gccggagcgg atcgagaatg acgggtacct ggagtacctg atccacaagc
      841 atcgcgcacg cattgtgctg accgccgtcg atgaagccca ctgcatctcg caatgggggc
      901 acgattttcg gccgccctac aagacgattc cgagcttcct ggaccgcgcg ttcggacgcg
      961 cccattggcc gacagtcgtc tgcctcacag gtacgctcaa taagggcgcg ctcgacgaga
     1021 tcttgtccga cttccggctt ggctccgaag acgtcgtgcg cagcggcacg atggtgcgga
     1081 gcaacctgaa gcttagcgtc caggccttcg ccgaccgcga tagcaagctc gccgctttgg
     1141 acgaattgct tgagacgcac cgagggaaga aactcattgt ctacgctcat ttgaaacaca
     1201 acaggattac gggtacgcgg gcgctgaccg atcgctactt ggcgaaaagg catcggtgcg
     1261 ccgcattcga cgcggacctc gggaccaccg agaaagatag ccttctgcgc gactttgagg
     1321 cgggcactat cgatgtcgtg tttgcgaccg gcgcattcgg tatgggaatc aatattcccg
     1381 acataagagg cgttatccat taccttcttc cggagtctat cgaacaatat taccaggagg
     1441 ttggacgcgc gggacgagac ggacagcccg cttttgggtg cctcttgtac acgtcgacca
     1501 acgcacgagt ccggcgcgat cagattaaga gcaaccgtcg taccaaagcg gacgtggaag
     1561 aggtgtggag agcggtctgc aatgagagtg taaactccct caagacagtg agtccgtggt
     1621 cggaattcaa gggccgcgaa gcggagtacg ccatcttcca gaacctggtt aggctgggcg
     1681 ccgtcgagat actagcacga gggccgaacc ggcttacctg tctcgaagcg cgcggggcca
     1741 acggagcggc aatgctgaat cggctttcgt ccgcgacgcg agccgggctt acagccgctg
     1801 ctatgcgaaa gcttggtgac gatccggtca ccacgataag caggctcttt gaactctacg
     1861 acgaacgaga agtcaccctc gtccagaacc ccgacaagac gctcttcttc cgcgcgcgcg
     1921 cgctctcgga ggaggctgta gtcgcgctgg tggagagttt ggacgcaaaa attgaccacc
     1981 gcttggcgga gtttgagacg ttggtatccc tcatcgaagg cggccacgat ctccaagcgg
     2041 tcctttccga gcggttcgac acataaatta ctgcgttgcc gcatacccct tcgccgaacg
     2101 tcggtggcgc agagttagcg gacgcgtgca tgccagatcc gatcgagcac agtggcccga
     2161 tgggcaatgt cccctaccca ttccggaccg gtctcatggc gcgcgcttga agcgtcctaa
     2221 ggcggaccgt attgttaggc gacggcactc ccaatggcac atcgagactt gagcatcgac
     2281 atcttcttcg gccgcgatat cggaaatatc gccgagcgcc gctgccttcg ccgcctcctt
     2341 tgccacctta cagccgtgca gaccgacgcc gtggtgctcg caaactttgt cgtcggaccc
     2401 cagcggcgac aggtcgatat ggtcgtcgcc acgcccaaga cggcggtcgt ggtcgagatc
     2461 aaaggttaca cacatcccgt tcacggcgat ctcaacggtc catggcacct ggaccttggc
     2521 gacggcaaga ctcgctcgct cggcccaacc aatccctatc atcaggcgct cgactgccgg
     2581 tacgccgtgt gcgacgcact cagcgctcac gccgacctcc aaggccatat cgcgcgcgcg
     2641 atggttcttg gcagtctttg cgtttttccg tcgccgctcg ccacatccgc attgcccgcc
     2701 tcagaccata aggttgtgat cggtggcgct cccgaattgg aggcgttgct cgagcggcct
     2761 cttccgacgg cgatggcgct cgacacatgg cgttccttcg cccgatcctt gcaactgtcc
     2821 cacggcggcg aggccgagcc ccgcccggcc tccacacttg tcgaaagcta tctcgccagg
     2881 ttcctggagc ttcgccgcgc ccttgctgtg ccctacatag aaccgctcat cgccggcgag
     2941 caggtcgcga catcgcatct cgtcggacgc cttgttgatg gggtacagct gcacatcgtt
     3001 ggcccctctg gttgaaccgc cccgggtttg tcggaggctc cagatcttga gagattggag
     3061 ctatggcgaa tcacaagacg tccccaaagt tttcagccga ggtgcgtgag cgcgcggtgc
     3121 ggatggtgtt ggagcaccgc gcagatcaca cgtcgcaatg ggcggcgatc tcctcgatcg
     3181 cgggcaagat cggctgcaca gcgcagaccc tgtcgaactg ggttcggcag gccgagcgcg
     3241 accagggccg acgccctggc gcgagcactg acgagcgcga gcggatcaag gcgttggagc
     3301 gggagaaccg cgagctgcgc caggccaacg agatcctgcg caaggccagc gcgtattttg
     3361 cccaggcgga gctcgaccgc cgatggaag
//
LOCUS       DASURG010000075         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_323363, whole genome shotgun sequence.
ACCESSION   DASURG010000075 DASURG010000000
VERSION     DASURG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323363"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFY80_01285"
     CDS             complement(<1..688)
                     /locus_tag="VFY80_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006562.1"
                     /translation="MGLAATRKLHLHRTYFAGRTIQRILSAADRFPASHPDAGCGTQR
                     GTVIVLRSLWMLAAACALSALQSASAQDADALRARHAALAGALSSNPFQRPLHIESAQ
                     ATDALRGDVYARLEQPHQKVANALADIDQWCDILILHLNVKSCRRSHAGSATTLVVLI
                     GRKYDQRPEQAYRVDFLYRIAAATPDYLRVVLNAESGPLGTKDYRIVLQAAALDAQRT
                     FVQLSYSYSYG"
     gene            715..996
                     /locus_tag="VFY80_01290"
     CDS             715..996
                     /locus_tag="VFY80_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006563.1"
                     /translation="MSAAPKKAMLAAMGAILRNDGLGSAGPIFPARPAFSFYEIALAT
                     AQYRDTHILSLGGGWYCYTVTPWVFTYRVPSWTEASVEAHRARIRRGLW"
     gene            complement(1016..2368)
                     /locus_tag="VFY80_01295"
     CDS             complement(1016..2368)
                     /locus_tag="VFY80_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006564.1"
                     /translation="MATETAKGASRAAGDWRARLPFFYGWIILGGSLIALGLTYSVMY
                     SFSVFYVALLEEFGWGRGEAAGIYSVFMIVTGLGALVSGALSDRFGPGRVLAAGALCL
                     GIGLMLASRTTALWHLYLSFGLVAGLGVSLAGWTPCAIMVSQWFSVRLGLALGIASGG
                     IGVGIMALVPLVQFLIGSLGWRSAYVALAAIVCLGLLPMGLFVLRGRPEDLGQPVDGI
                     APESAQLRKSGARRTRKEMHIVDAEWANRAWTVPTAVRTMRLWLLSAVKLLGGVATQM
                     IFVHQVVFLVENGYDRMLAASVVGIIGFLSVGAKVLWGWAADNIGREMTYTLGCGAMI
                     VAVGLLALAAVAPSPAVLYLYAAIFALGYGVSAPMWPIVTSDLFAGRKFGSIYGVISV
                     FSGFGNALGAWVGGFVYDLTGSYAIAFGVAIAAKAASAGALWIVAPRKVRRVQLMRGA
                     "
BASE COUNT          519 a          849 c          780 g          420 t
ORIGIN      
        1 agccatagct gtacgaatac gacaactgaa cgaatgtccg ctgcgcatcg agtgccgccg
       61 cctgcaatac gatacggtag tccttcgtgc cgagcggtcc ggattcggca ttgagaacca
      121 cacgcagata atcgggcgtc gcagcagcga tcctgtagag aaagtccacg cggtaagctt
      181 gttccggtcg ctggtcgtac tttcttccga tgagaaccac cagggtggtt gcggacccgg
      241 cgtgcgatct tctgcagctt ttcacgttga gatgcaggat caggatgtcg caccactggt
      301 cgatgtccgc caacgcgtta gcgaccttct ggtggggttg ttccagcctc gcatagacgt
      361 caccgcgcaa agcgtcggtg gcctgagccg attcgatgtg cagcggacgc tggaacgggt
      421 tactgctgag cgctccagcg agcgccgcgt ggcgagcacg aagcgcgtcg gcgtcctgag
      481 cgctcgcgct ttgtaaagcg ctcaaagcac aggccgcggc cagcatccat aagcttcgca
      541 acacgattac cgtgcctcgc tgcgttccgc agcccgcatc gggatgcgag gcgggaaagc
      601 ggtccgccgc cgataaaatg cgctggatgg tgcgtccggc gaaataagtc cgatgaagat
      661 gcagcttacg cgtcgcggct aagcccatgt tgatcctcgt caacctcgaa gggcttgagc
      721 gcagcgccga aaaaagcgat gctagcggcc atgggcgcca tcctcagaaa cgacgggctc
      781 gggagcgcgg ggccgatctt cccggcccgc ccggcatttt cgttctacga gatcgcgctc
      841 gcgaccgcgc agtaccggga cacccacatc ctgagcctgg gcggcggctg gtactgctat
      901 accgtgacgc cgtgggtctt cacctaccgc gtaccgagct ggaccgaggc gagcgttgaa
      961 gcgcaccgcg cgcggatccg gagggggtta tggtgagcgt cgaaggccgc cgatatcagg
     1021 cgccacgcat cagttgcact cttctgactt tgcgcggcgc gacgatccac agcgcgccgg
     1081 cgcttgcggc tttggcggcg atggccacgc caaaagcgat cgcgtagctc ccggtcaggt
     1141 cgtacacgaa gccgcctacc cacgcgccca gcgcgttgcc gaagccgctg aatacgctga
     1201 tcacgccgta gatcgagccg aacttgcgtc cggcgaagag atcggaggtc acgatcggcc
     1261 acatcggtgc cgatacaccg tatccgagcg cgaagatcgc ggcatacaga tacaggactg
     1321 ccggggacgg tgcgaccgct gccagggcga ggagtcccac ggctacgatc atggcgccgc
     1381 atcccagcgt gtaggtcatc tcccggccga tgttgtcggc ggcccatccc cagagcactt
     1441 tcgcgcccac actcaagaat ccgatgatgc cgacgacgga ggcggcgagc atccggtcgt
     1501 agccgttttc cacgagaaag accacttggt ggacgaatat catctgcgtc gcgacgccgc
     1561 ccaggagctt gaccgcagag agcagccaga ggcgcatcgt ccgaaccgcc gtcggcactg
     1621 tccatgcgcg attcgcccat tcggcgtcca cgatgtgcat ttccttgcgg gtacgccgcg
     1681 cgcctgattt cctgagctgc gcgctctccg gtgcaatacc gtcgaccggt tggcccagat
     1741 cttcgggccg tccgcgcagc acgaagagcc ccatcggcag caggcccaag cacacgatcg
     1801 ctgcgagcgc aacgtaggcg ctgcgccaac cgagactccc gatcagaaac tggacgagcg
     1861 gcacgagcgc catgatgccc acgccgattc cgcccgatgc gatgcccagg gcgaggccga
     1921 gccttaccga gaaccattgg ctcaccatga tcgcgcacgg cgtccatccc gcgaggctca
     1981 ctccaaggcc ggcgaccagc ccgaacgaga gatagagatg ccacaacgcg gtggtgcgac
     2041 tcgcaagcat gaggccgatc ccgaggcaga gcgcgcccgc tgcaagcacg cgcccgggtc
     2101 cgaaccggtc ggaaagcgcg cccgaaacga gcgcgccgag gccggtcacg atcatgaaga
     2161 cggaatagat cccggccgcc tctccgcgcc cccaaccgaa ctcctccagc agcgccacgt
     2221 aaaacaccga aaaggagtac atgacggagt aggtcaggcc aagcgcgatg agactcccgc
     2281 cgagaatgat ccagccgtag aaaaaaggca gccgcgcccg ccagtcaccc gcagcccgac
     2341 tcgctccctt tgccgtctca gtggccatcg aaccctctaa ctagcgcatc gtggtatcag
     2401 cggtactgac ataacgcaaa cggactgcaa gtcgattggc tcatatcaag cggcctgcgc
     2461 gccttgatgt catactttca cggtcggctt tatatcctcc cacgatgaac cgcaccaaaa
     2521 tcgccgcctg gctacgccgc atattcctgt ccctcccgtt cgcgattg
//
LOCUS       DASURG010000076         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_221200, whole genome shotgun sequence.
ACCESSION   DASURG010000076 DASURG010000000
VERSION     DASURG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221200"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="ccmC"
                     /locus_tag="VFY80_01300"
     CDS             complement(<1..662)
                     /gene="ccmC"
                     /locus_tag="VFY80_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX6006565.1"
                     /translation="MSHPGEVAEGPRPTWGRINWFKYSSPQSFFPLAGDAIPWFAVAA
                     ALLCAAGLYVGFFVAPTDQQQGDAYRIIFIHVPAAWMSMFIYLVMAFWAGVGLVLNTR
                     LSHMMANALAPTGALFTFIALWTGALWGKPTWGAWWVWDARLTSELILLFIYFGYMAL
                     GAAIDDPRRADRAAALFALVGVVNIPIIYFSVQWWNTLHQGASVSLTRAPTMAATMFL
                     GM"
     gene            complement(739..1407)
                     /gene="ccmB"
                     /locus_tag="VFY80_01305"
     CDS             complement(739..1407)
                     /gene="ccmB"
                     /locus_tag="VFY80_01305"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX6006566.1"
                     /translation="MLTVIGVVIGRDLKLAMRRRSDVANALLFFIVVASLFPLGVGPE
                     PNLLRTIAPGVIWVAALLASMLALGRMFAADYADGALEQMLLGAAPLGAIVAGKVIAH
                     WLVTGLPLMVLAPLIAIQFDLPPGALGVLVLSLAIGTPGLTLIGAIGAALTLGVRGSG
                     VLTSLLVLPLYVPVLILGTGAVDAAAGGVAVQAHLLLLAAFLVFAAAFAPWATAGALR
                     ISLD"
     gene            complement(1415..2032)
                     /gene="ccmA"
                     /locus_tag="VFY80_01310"
     CDS             complement(1415..2032)
                     /gene="ccmA"
                     /locus_tag="VFY80_01310"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX6006567.1"
                     /translation="MLEAIDLACERGERRLFDGLDFKLEPGTLLRIAGANGSGKTSLL
                     RILCGLALPAHGQVRWEGESIRNLREEYHRELTYIGHLPAVKDDLTARENVMISCALG
                     GAPVDEAAADAMLERVGLARYAEYPARHLSQGQRRRVALARLAARRPNRLWVLDEPFT
                     ALDAVAVTLVVDMIAEHLALGGAAVITSHQEAMLASVTTTIIDLG"
     gene            complement(2167..>2511)
                     /locus_tag="VFY80_01315"
     CDS             complement(2167..>2511)
                     /locus_tag="VFY80_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006568.1"
                     /translation="RQHWGMQRYVAGLGEREAENLAAFYAAQERGAADVVPTSAKELA
                     DKCNRCHDADDNAKVAMPKIRGQDKDYLVMALRAYRDDRRASSAMHRMSSVYSNAIIE
                     DIASYYAAQPAQ"
BASE COUNT          502 a          842 c          787 g          380 t
ORIGIN      
        1 atcattccga ggaacatcgt tgccgccatc gtcggcgcgc gcgtgaggct gaccgatgcg
       61 ccctggtgca gcgtgttcca ccattggacc gagaaataga tgatcgggat gttgacaacg
      121 cctaccagcg cgaagagcgc cgccgcgcgg tcggcacggc gcgggtcgtc gatcgccgca
      181 cccagcgcca tgtagccgaa atagatgaag agcaggatca gctcggatgt gagccgcgca
      241 tcccacaccc accatgcgcc ccaggtcggt ttgccccaga gcgcgcccgt ccacagcgcg
      301 atgaaggtga agagtgcacc ggtcggcgcc agcgcattcg ccatcatgtg cgaaagccgc
      361 gtgttcagca cgagcccgac accggcccag aatgccatca ccagatagat gaacatcgac
      421 atccaggcgg ccggcacatg gatgaagatg atgcgatacg cgtcgccctg ctgctggtcg
      481 gtcggcgcca cgaaaaaccc gacgtagagg cccgctgcgc agagcagcgc ggcagccacg
      541 gcaaaccatg ggatggcgtc gcccgccagt gggaaaaaag actggggtga cgagtacttg
      601 aaccagttta tccggcccca cgtcgggcgt ggaccttctg cgacttcacc gggatgagac
      661 atcagcacga atcataaaaa tttcgaggta ctctaaggca attcgcccct ctgcggttga
      721 tttgcatcaa cctgcaggct agtcgagcga aatcctgagc gcccccgccg tcgcccacgg
      781 cgcaaacgcg gcggcaaata caaggaacgc cgcaagcagg agcagatgcg cttgcacggc
      841 cacccctccc gctgccgcat cgaccgcgcc ggtccccaga atcaacaccg gaacgtaaag
      901 cggaagcacc agcagcgacg tgagtacgcc tgagccgcgc acacccaagg tgagcgctgc
      961 accgatcgcc ccgatcagcg tcagcccagg ggttccgatc gcgagcgaca acacgagaac
     1021 gccgagcgcc cccggcggca ggtcgaattg tatcgcaatg agcggtgcga gaaccatgag
     1081 cggcagcccc gtgacgagcc agtgtgcgat caccttgccg gccacgatcg cgccaagcgg
     1141 cgccgcgccg agcagcatct gctccagggc gccgtccgcg taatcggcgg cgaacatgcg
     1201 acccagggcc agcatggaag ccaggagcgc cgccacccaa atgacaccgg gcgcaatggt
     1261 gcgaagcaga ttcggctccg gtcccacgcc gagcgggaaa aggctcgcga cgacgataaa
     1321 gaacagcagc gcattcgcaa cgtccgagcg gcgccgcatc gcgagcttga gatcccggcc
     1381 aatcactacg ccgatgacgg tcagcacgtg ctcgtcaccc gagatcgatg atggtcgtcg
     1441 tcaccgaagc gagcatcgct tcctggtggc tcgtaatgac ggcggcgccc ccgagcgcca
     1501 ggtgctccgc gatcatgtcc acgaccagcg tgacagcgac cgcatcgagc gcggtgaatg
     1561 gctcgtcgag cacccagaga cgattcggtc ggcgcgccgc gaggcgcgcg agcgccacgc
     1621 gccgccgctg gccctgcgag agatggcgcg ccggatactc ggcatagcgc gcaagcccca
     1681 cgcgctcgag catcgcatcc gcagccgcct catccaccgg cgctccgccg agcgcgcagg
     1741 aaatcatcac gttttcgcgc gccgtcagat cgtccttcac cgccggaaga tggccgatgt
     1801 aggtcaactc gcggtgatac tcctcgcgca gatttcgtat cgactcgcct tcccagcgca
     1861 cttggccgtg cgccggaagt gcgagtccgc acagaatgcg aaggaggctc gtcttgccgc
     1921 tgccgttcgc cccggcgatg cgcaggagcg tgcccggttc aagcttgaaa tcgagaccgt
     1981 cgaacagcct gcgctctccg cgctcgcagg cgagatcgat cgcttcaagc atggcgcgcg
     2041 cgggccggta ctgtcactta agcattcctg tgtcggaaag tcgcgggcgc cattctatcc
     2101 ggtttcgagg acgtactgac gcatgcgcgg tgcgcatggc agcgcgattc agccggcaag
     2161 ctcagactac tgcgcaggct gggccgcgta gtatgaagcg atgtcttcga tgatggcgtt
     2221 gctgtagacg gagctcatac ggtgcatcgc cgagcttgcg cgccggtcgt cgcgatacgc
     2281 gcgaagcgcc atgacgagat agtccttgtc ctggccgcgg atcttcggca tggcaacctt
     2341 ggcgttgtca tcggcgtcgt ggcaacgatt gcacttatcc gccagctcct tggcggaagt
     2401 cggcaccacg tccgccgcgc cgcgctcctg cgctgcgtag aaagcagcga gattctccgc
     2461 ctcgcgctcg ccgagccccg caacgtaacg ctgcatgccc caatgctggc g
//
LOCUS       DASURG010000077         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1038211, whole genome shotgun sequence.
ACCESSION   DASURG010000077 DASURG010000000
VERSION     DASURG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038211"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFY80_01320"
     CDS             complement(<1..606)
                     /locus_tag="VFY80_01320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEX6006569.1"
                     /translation="MSAIRIGILDQSPIISGHSPAEAVHETIRLAQAAERLGYHRYWL
                     AEHHSTAALADPCPEILVTRVAAATTRIRVGTGGVLLPYYSAFKVAEQFRMLEALYPG
                     RIDLGLGRAPGGDRLTALAVAEGKLLDAESFPEQVRYVAAFLSDSLPRDHPLAAVKAM
                     PAGDTVPQIWLLGSSDYSGALAARLGLRFAFAHFINADGGDA"
     gene            complement(660..1616)
                     /locus_tag="VFY80_01325"
     CDS             complement(660..1616)
                     /locus_tag="VFY80_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006570.1"
                     /translation="MKTEVSLYVGEALGKYGFPNGHPFGPDRQDAFWKEAVKQGLDKE
                     AALCEPRPAAREEIRRFHTDEHVERVDHLSRLGYGSIDYGDTPAYPGVFDASSAVVGA
                     ALEGLERVMSGETYRTFQPIGGLHHARRGTAAGFCVFNDCGVVIDTLRELYGLKRIAY
                     VDIDVHHGDGLYYPYEDDPALIYADIHEDGNYLYPGTGHDFERGTGGAEGVKLNIPMQ
                     PGWGDKEFVRAWERVVNHLARYEPEFIVMQCGADSLAGDPLAHLQYTPAAHAHAARSL
                     CELANRYARGRIMGFGGGGYNRANLAAAWCAVLQEFIRGSER"
     gene            complement(1675..>2580)
                     /locus_tag="VFY80_01330"
     CDS             complement(1675..>2580)
                     /locus_tag="VFY80_01330"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX6006571.1"
                     /translation="RNESPDIPFRQSINPYEGCEHGCGYCYARPSHAYRNLSPGIDFE
                     TRLFAKVNAAEVLRAELARPNYRCDVISLGANTDPYQPIERRYGITRGILEVLAEFNH
                     PVGIVTKNAMIERDLDILAPMAEKRLVNVYISCNNLDHELARRLEPRCSAPRRRLRAM
                     KRLSEAGVPVGVLVAPVIPFLTDHEIEAVLEAAWAHGARQAGYVLLRLPWEIKHIFRD
                     WLERHYPLKVKHVMSRVQAMRGGRDNDPHFGTRMSGTGELAELLERRFEVACRKLGFN
                     HGSRYVTLDTTKFAVPGSGRQKTLF"
BASE COUNT          449 a          850 c          820 g          461 t
ORIGIN      
        1 ggcgtcgccg ccgtcggcgt tgatgaaatg cgcgaatgcg aatctcagcc cgagcctcgc
       61 cgccagcgcg ccgctgtagt ccgaggagcc gagcagccag atctgcggca ccgtgtcccc
      121 cgccggcatc gccttcaccg ctgcgagcgg atggtcgcgg ggaagcgagt cgctcaggaa
      181 agccgcgacg taccgcacct gctcgggaaa gctctcggcg tcgagcagct tgccttccgc
      241 caccgcgagc gcggtgagcc ggtcgccgcc cggcgcgcgc ccgaggccga ggtcgatgcg
      301 gccgggatag agcgcctcca gcatgcggaa ctgctcggcc accttgaatg cactgtaata
      361 aggaagcagc acgccgcccg tgcccacgcg tatccgggtc gtggccgcag cgacgcgcgt
      421 cacaagtatt tcgggacagg gatcggcaag cgcggcagtc gaatgatgtt cggcgagcca
      481 gtagcggtgg tagccgagcc tttcggccgc ctgcgcgagg cggatcgttt catgcactgc
      541 ttcggcgggg gaatgaccac tgatgatcgg cgactggtcg aggatgccga ttcggatggc
      601 gctcattctg tcttatagca gccggaatgc gtgctttggg gacttcgttt ccgcgcttgc
      661 taccgctcgg agccgcggat gaattcctga agcaccgcac accacgccgc ggcgaggttc
      721 gcccggttgt agcccccgcc gccaaagccc atgatcctgc ctctcgcata cctgttcgca
      781 agctcgcaca ggctgcgcgc ggcgtgggca tgcgccgcgg gcgtgtactg caggtgggca
      841 agcggatcgc ctgcgagact gtctgcgccg cactgcatga cgatgaactc cggctcgtaa
      901 cgcgcaagat gattcaccac gcgttcccat gcgcgcacga attccttgtc gccccagcca
      961 ggctgcatgg gaatgttgag cttcacgccc tccgccccgc ccgtcccgcg ctcgaagtca
     1021 tgaccggtcc cgggatacag atagttgcca tcttcgtgga tgtcggcgta gatgagggcg
     1081 gggtcgtctt cgtacgggta atagagtccg tcgccgtgat gaacgtcgat gtccacataa
     1141 gcgatgcgct tcagcccgta taactcgcgc aaggtgtcga tcacgacgcc gcaatcgttg
     1201 aaaacgcaaa agcccgccgc cgtgccgcgc cgcgcgtgat gcagcccgcc gatcggctgg
     1261 aacgtgcggt atgtttctcc gctcatgacg cgctccaggc cttcgagcgc agcgccaacg
     1321 accgcggacg aggcatcgaa cacgccggga tacgccggtg tgtcgccata gtcgatcgaa
     1381 ccgtatccaa gacgcgagag atgatccacg cgctcaacgt gctcatcggt atggaagcgc
     1441 cggatctcct cgcgggccgc agggcgcggc tcgcacagcg cggcctcctt gtcgagccct
     1501 tgcttcaccg cctccttcca gaacgcgtct tgacggtcag gcccgaaagg atggccgttc
     1561 ggaaagccat acttgccgag cgcttccccg acataaagcg aaacttcggt cttcatcgcg
     1621 attcctctga tcccgaccgc cccgcacggc cgtcgttcaa gatcggcgcg tacgtcagaa
     1681 aagcgtcttc tgccggccgg agccgggcac ggcgaacttc gtcgtgtcca gtgtaacgta
     1741 gcggctgccg tgattgaatc cgagcttgcg gcacgccact tcaaaacggc gctcgaggag
     1801 ctcggcaagc tcgcccgttc cgctcatgcg cgtgccgaaa tgggggtcgt tgtcgcgtcc
     1861 tccgcgcatc gcctgcacac ggctcatcac gtgcttcacc ttgaggggat agtgccgctc
     1921 gagccagtcc ctgaagatgt gcttgatctc ccaaggcagc cggagcagca cgtagcccgc
     1981 ctgacgcgca ccgtgcgccc acgcggcttc gagcaccgct tcgatctcgt gatcggtgag
     2041 aaacggaatc accggcgcga ccagcacgcc gaccggcaca ccggcttcgg acagccgctt
     2101 catcgcccgc agccgtcgcc gtggcgccga gcagcgcggc tccagccgcc gcgcgagctc
     2161 gtgatcgagg ttgttgcacg aaatgtatac gttgacgagc cgcttctccg ccatcggggc
     2221 gaggatgtcg agatcgcgct cgatcatcgc attcttggtg acgatgccaa ccgggtggtt
     2281 gaactcggca agcacttcga ggatgccgcg cgtgatcccg tagcggcgct cgatcggctg
     2341 atacgggtcg gtgttggcgc cgagcgaaat tacgtcgcac cgatagttcg gcctcgcgag
     2401 ttcggctcgc agcacttccg ccgcgttcac tttcgcgaag agccgcgtct cgaagtcgat
     2461 ccccggcgat agattgcgat aggcgtgaga agggcgagcg tagcagtatc cacacccgtg
     2521 ctcgcatccc tcgtacggat tgatggattg cctgaaaggg atgtccggcg actcattgcg
//
LOCUS       DASURG010000078         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_833701, whole genome shotgun sequence.
ACCESSION   DASURG010000078 DASURG010000000
VERSION     DASURG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833701"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..695
                     /gene="recR"
                     /locus_tag="VFY80_01335"
     CDS             99..695
                     /gene="recR"
                     /locus_tag="VFY80_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX6006572.1"
                     /translation="MKTPSSLDELIEALRCLPGVGPKSAQRMAYHLLQRDTPGAQRLA
                     SALADALGRIRHCEKCNSFAEEPVCGLCRSPRRDATQLCVVESPGDLLVMEQAQCFHG
                     LYFVLMGTLSPLDGIGPKEIHLDRLIRRATDGVVREVILATNFTVEGEATAHYVAELL
                     REQGIKVSRIARGLPVGGELEHVDSGTLAQAVIERRGV"
     gene            692..1558
                     /locus_tag="VFY80_01340"
     CDS             692..1558
                     /locus_tag="VFY80_01340"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX6006573.1"
                     /translation="MSSRPKSAGEARHGPVARADTTQKLHKLLAQAGLGSRRAMEEWI
                     RGGRVTVNGTVATIGTRVSPRDIVRVGRRIVRWPREERLPRVVLYHKPEGEIVSRADP
                     EERTSAFDRLPPVRGGKWIAVGRLDYNTGGLLVFTTSGEVANRMMHPRYALEREYALR
                     VLGTLKPEQLARLKSGIELADGPAHLVSIEDEGGEGANHWYRIVLAEGRNRIVRRIFE
                     ALGVTVSRLMRVRFGCIVLPRRLRRGQWLELPPAEVKALLRELGLTYSPPANPRGVPQ
                     KRNHHRRSSPAR"
     gene            complement(1479..2405)
                     /gene="prmB"
                     /locus_tag="VFY80_01345"
     CDS             complement(1479..2405)
                     /gene="prmB"
                     /locus_tag="VFY80_01345"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX6006574.1"
                     /translation="MQRRKPRRASSPDLPSRTVAELIRCGESRFKKARLCFGHGLPSA
                     YDEAAFLVLHVLKLSPATLETRLEERPSPAETRKILRLFRERVEWRKPAAYLTGEAWL
                     GGLRFRIDENVIVPRSYIAELLSDALSPWVLRPRQVRSALDLCTGSGCLAILLAHAFP
                     KAGIDAADISPRALRIARHNVSDYGLEERVRVIRSNLFAALAGRRYDVIVSNPPYVAA
                     ASLRRLPPEYRHEPEIALAGGGDGLDFVHAILRDAGRHLNRHGWLVVEAGHNRERLER
                     AFPRTPFIWPVTSGGDDCVFVVRREDLPAGNT"
BASE COUNT          422 a          792 c          850 g          399 t
ORIGIN      
        1 ccgcgatcac tgcaggcatg ccgatgccgc ccggcttcaa gatgccgttc tagtcgcgta
       61 tctccgcatc cggcttaccc tgcctttcgc tggatctcgt gaagactccg tccagcctcg
      121 atgagctgat tgaggcactg cgctgccttc caggtgtggg gccgaagtcg gcgcagcgca
      181 tggcgtatca cctgctgcag cgggacactc ccggcgcgca gcgactggcg agcgcgctgg
      241 ccgatgcgct cggccgcatc cggcattgcg agaagtgcaa cagctttgcc gaagagccgg
      301 tctgcgggct gtgtcgttcg ccccggcgcg atgcaactca actctgcgtc gtcgagagtc
      361 cgggcgatct gttggtcatg gagcaggcac agtgctttca cggcctctac ttcgtgctga
      421 tgggcacgct gtccccgctc gacggcatcg ggccgaaaga aatccatctc gaccgcctca
      481 ttcggcgtgc gacagatggt gttgtgcgcg aagtgatcct cgcgacgaac ttcaccgtcg
      541 aaggcgaagc gaccgcgcat tacgttgcgg agctgctccg cgagcagggg atcaaggtga
      601 gccgcatcgc gcgcgggctt ccggtcggcg gcgagctcga gcacgtggac agcgggacgc
      661 ttgcgcaagc tgtcatcgag cggcgcggcg tgtgagttca cgcccgaaat cggcgggcga
      721 ggcccggcac ggtccggtcg cgcgcgccga taccacgcag aagctgcaca agctcctcgc
      781 gcaggcgggc ctcggctcgc gccgcgccat ggaggagtgg atacgcggcg gacgcgtgac
      841 ggtgaacggg acggtcgcca cgatcggcac gcgcgtatcg ccgcgcgaca tcgtgcgcgt
      901 cgggcggcgc atcgtgcgct ggccccgcga agagcggctc ccgcgggtgg tgctctacca
      961 caagcccgaa ggcgaaatcg tgtcgcgagc agatcccgaa gagcgcacca gcgcgttcga
     1021 tcgcctgccc cctgtacggg gcggcaaatg gatcgccgtc gggcgcctcg attacaacac
     1081 cggaggcctg ctcgtcttta cgacctccgg cgaagtcgca aaccgcatga tgcacccacg
     1141 ttacgcgctg gagcgcgaat acgcgctgcg cgttctcggc acgctcaagc ccgagcagct
     1201 cgcgcgactc aagagcggaa tcgagctcgc agacggcccg gcgcacctcg tctcgatcga
     1261 ggacgaaggc ggtgaaggcg cgaatcactg gtatcgcatc gtgctcgcgg aagggcgtaa
     1321 ccgcatcgtc cgccgcatct ttgaagcgct gggcgtcacg gtaagccggc tgatgcgcgt
     1381 gcgtttcggg tgcattgttc tgccccgccg gctgcggcgc gggcagtggc tcgagctgcc
     1441 gcctgcggag gtgaaggcgc tgctacgcga gctcggcctt acgtattccc cgccggcaaa
     1501 tcctcgcggc gtaccacaaa aacgcaatca tcaccgccgc tcgtcaccgg ccagatgaag
     1561 ggcgtgcgcg gaaaggcgcg ctccagccgc tcgcggttgt gaccggcctc caccacgagc
     1621 cagccgtggc ggttgagatg gcggcccgca tcgcgcagga tcgcgtgcac gaagtcgagg
     1681 ccatcgcccc cgcctgccag cgcgatctcg ggctcatgcc gatattccgg gggaaggcga
     1741 cgcagggagg cggccgcgac gtaaggcggg ttggaaacga tgacgtcgta gcgccggcct
     1801 gccagcgcgg cgaagagatt cgagcggatg acacgcacgc gttcttcgag accgtagtcg
     1861 gatacgttgt ggcgggcgat gcgcagtgca cggggcgaga tgtccgccgc atcgatcccc
     1921 gccttgggga aagcgtgcgc gagcagaatc gcgagacagc ccgatcccgt gcagagatcg
     1981 agtgccgagc gaacctgtcg cggtcgaagc acccacggac tcagcgcatc cgagagcagt
     2041 tccgcgatgt aggagcgcgg aacgatgacg ttctcgtcga tgcgaaagcg cagtccgcca
     2101 agccatgctt ccccggtgag ataggcagcg ggcttgcgcc attcgacccg ttcgcggaag
     2161 agtcgcagga tcttccgggt ctctgcgggc gaagggcgct cttcgagccg ggtctccagg
     2221 gtagccgggg agagcttgag gacgtgcagt accagaaacg ccgcctcgtc gtaggcgctt
     2281 ggaaggccgt gaccgaagca cagtcgcgct tttttgaagc ggctctcacc gcaacggata
     2341 agctccgcta cggtgcgcga gggaagatcc gggctggagg cgcgccgcgg ctttcttctc
     2401 tgcacgcggc ccgttatccg agggcgtcga gattcagctt gatgttcgag agatcatccg
     2461 cgc
//
LOCUS       DASURG010000079         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_119749, whole genome shotgun sequence.
ACCESSION   DASURG010000079 DASURG010000000
VERSION     DASURG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119749"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFY80_01350"
     CDS             complement(<1..768)
                     /locus_tag="VFY80_01350"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEX6006575.1"
                     /translation="MCGIVGYIGDKRAAPLLIQGLRRLEYRGYDSAGIAVQVNGHVET
                     KKSVGKIVELEKILSNGGEPAGTVGIAHTRWATHGAPNTTNAHPHIDCKNEIAVVHNG
                     IIENASTLKQKLTALGHTFTSETDTEVVAHLIEEAFEGNLEEAVRAALQQVEGAYGLA
                     IISSRDPNKIVVARKGSPLLLGITDNGEYFVASDVAAVLAQTRQVIYLDDGEMAVLTR
                     DGYRTLSLRGGEITKEIAHVAWDLDAIEKGGHEHFMLK"
     gene            complement(847..2238)
                     /gene="glmM"
                     /locus_tag="VFY80_01355"
     CDS             complement(847..2238)
                     /gene="glmM"
                     /locus_tag="VFY80_01355"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6006576.1"
                     /translation="MTMQFELPKELMVSVSGVRGRVAEALTPEIVARFTAAYGTYLRT
                     ERKKRPQVIVGRDSRTSGPMFVRAVVSALQSVGCDVREVGIAPTPTCLYAIRHHQADG
                     AIVVTASHNPVEWNALKFASWESMFLDAEQAPRMREYLPEEKIHRAQWDELGVVDVDN
                     DAVTRHINAVLATRYLDVPALQRRRFTVALDCVHGAGSVLLAPLLQALGCTVHVMGGA
                     PDGRFPREPEPVAANLGELERLVRETGADIGFATDPDADRLSLVSNEGKAVGEDFTLA
                     LASMLVLRHRKGPVVTNLSTSRVLEDVAAQAGVPLIRAAVGEINVARRMEKEKAVIGG
                     EGNGGVILPDVHLTRDSAVATSLILQLLLESDKSVAQLVKEQTPYVIVKDKLPRDSGS
                     LEDAFAKLESKLKAAEADRQDGLRLAWPAERRWLHLRASGTEPILRIIAEAPDEKGAR
                     ELVSQARTALGVK"
BASE COUNT          424 a          889 c          771 g          506 t
ORIGIN      
        1 cttgagcatg aagtgctcat gcccgccctt ctcgatcgcg tccagatccc acgcaacgtg
       61 cgcgatctcc ttcgtgatct ccccgccccg cagcgatagc gtgcgatagc cgtcgcgcgt
      121 cagcaccgcc atctcaccat cgtcgagata gatgacctgg cgcgtctgcg ccagcaccgc
      181 ggccacgtcg ctggccacga aatactcgcc gttgtccgtg ataccgagca gcaacggact
      241 gcctttgcgc gcaaccacga tcttgttcgg atcccggctg ctgatgatgg ccaggccgta
      301 tgcgccttcc acctgctgca gcgctgcccg caccgcttcc tcgagattgc cctcgaaggc
      361 ttcctcgatc aggtgcgcca cgacctccgt atcggtctcg ctcgtgaacg tgtgaccgag
      421 tgcagtcagc ttctgcttca gcgtgctggc attctcgatg atgccgttgt gcactacggc
      481 gatctcgttc ttgcagtcga tatgcgggtg cgcgttcgtc gtgttgggcg cgccatgcgt
      541 ggcccagcgc gtatgcgcaa tgccgaccgt gcccgccggc tcgccgccgt tgctcaggat
      601 cttctccagc tcgacgatct tgccgaccga ttttttggtt tcgacgtggc cgttcacctg
      661 cacggcgatg cccgccgagt catagccgcg gtactccagg cggcgcagcc cctggatcaa
      721 cagaggtgcc gcccgcttat cgccgatata gcctacaatt ccacacatat ttgatctggg
      781 gaaaatggac cttcctgccg cacccgtgtg gtgtcagccc ggcttaccaa tgcaaaggct
      841 ctgccgttat ttgacgccca gggcggtccg ggcctggctg accagctcgc gcgccccttt
      901 ttcatccggc gcctcggcga tgatgcgtag aatcggctcc gtccctgacg ctcgcagatg
      961 cagccagcgg cgttcggcgg gccaggccag cctcaaaccg tcctgccggt cggcctcggc
     1021 tgccttcagc ttcgactcca atttcgcgaa cgcatcctcc agggaacccg agtcgcgcgg
     1081 cagtttgtcc ttcacgatga cgtacggcgt ctgctctttt acgagctgcg ccacactttt
     1141 gtcgctctcc agcaacagtt gaagaatgag cgacgtcgcc accgccgagt cacgcgtgag
     1201 gtggacatcc ggcaggatca cgccgccgtt gccttctccg ccaatcaccg ctttttcctt
     1261 ttccatgcgg cgtgcaacat tgatctcacc caccgccgcg cgaatgagcg gcacgcccgc
     1321 ctgtgcggcc acgtcttcca gcacgcgact ggtggacaga ttcgtcacga ccggtccctt
     1381 gcgatggcgc agtacgagca tgctggccag cgcgagcgtg aaatcctcgc ctacggcctt
     1441 gccttcgttg ctcaccagtg acaagcggtc cgcatccgga tcggtggcaa acccgatgtc
     1501 ggcaccggtc tcgcgcacca gacgctcgag ctcgccgaga ttcgcggcca ccggctccgg
     1561 ctcacgcgga aaacgtccgt cgggtgcgcc gcccatgacg tgcaccgtgc agccgagtgc
     1621 ctgcagcagc ggcgcgagca gcaccgagcc ggccccgtgc acgcagtcga gcgccaccgt
     1681 gaagcgccgg cgctgcaagg ccggcacatc gagatagcga gtggccagca cagcattgat
     1741 atgacgcgtg acggcatcgt tgtcgacgtc gacgacaccc agctcatccc actgcgcgcg
     1801 atgaatcttc tcttccggca gatactcgcg catgcgcggc gcctgctccg catccaggaa
     1861 catgctctcc catgatgcga acttgagcgc attccattcg accggattgt ggctcgcggt
     1921 cacgacaatc gcgccgtccg cctggtgatg ccggatggcg tacagacagg tcggcgtcgg
     1981 tgctatgccc acctcgcgca catcgcagcc gaccgattgc agggccgaca cgaccgcgcg
     2041 cacgaacata gggccggagg tgcgtgagtc gcgtccgaca atcacctgcg gccgcttctt
     2101 tcgttcagtt cggagatatg tgccgtaggc cgcggtgaag cgcgccacga tttccggcgt
     2161 gagtgcctcg gccacgcggc cgcgtacgcc ggagacggag accatcagtt ctttcggtag
     2221 ctcgaattgc atcgtcatga ctgccggttt gagacgggcc ggatattagc gagcaggcat
     2281 ggggtgcgcg agggaggggt tcgacgccat attgcatgtc cgatcgtcac tcgcccagcc
     2341 aaggagtcgc cctcagcatg cagtctcgca gcaatacatt cccggccgtc gccctcttcc
     2401 ttgtttgttg gacctgcccg ctcactgcac aacagcccgt cgcaccggcc agcgtcaccg
     2461 ctgagcagac cggagccggt gaagtgcggc tacgctggtc ggcggtgccg ggtgcgcagg
     2521 cgtatgtcat ccagcgctcg gtctggccga atggctttca gcgcttcgtg cctgagggcg
     2581 tggccgaaac
//
LOCUS       DASURG010000080         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_426071, whole genome shotgun sequence.
ACCESSION   DASURG010000080 DASURG010000000
VERSION     DASURG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426071"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFY80_01360"
     CDS             complement(<1..1035)
                     /locus_tag="VFY80_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6006577.1"
                     /translation="MEMLAGLMHGFAVALEPMNLFWCFVGVFFGTVVGILPGLGPSAT
                     VALLLPLAMQMNPTSGLIMMAGIYYGAKYGGSTTSILLNVPGESASVVTCLDGYEMAR
                     RGRAGPALGIAAISSFVAGTIGVLLLMLVAPPVAEFALRFGPPEYFALMAMGLAMVVL
                     LAGKSMIKALLSMFLGLLLATVGVDLFTDNARFTFDRVELVDGVNFVVVAIGTFAIGE
                     VLANLTSTGGGQLFNVPKGLRNLLPNLQDLKDSRFAFLNGSLVGFFVGVLPGAGSTIA
                     SFVSYGIEKAVSRHPEKFGTGAVEGVAAPEAANNSETGGALVPLLTLGIPGSGTTAIL
                     LVALVMWGLRP"
     gene            complement(1048..1521)
                     /locus_tag="VFY80_01365"
     CDS             complement(1048..1521)
                     /locus_tag="VFY80_01365"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6006578.1"
                     /translation="MSPRKSIDRSELVASVTLIALGLFAIHEARAWEYMTHDGPGPGF
                     FPLWIGIAMVTLSALYLASHAYDVMRGQPVHRTGWSGTALVIAGWAAFMVVVALIKPI
                     GFIAALIVLVVIYVRVIYRRSYITTIATAAACALSFWFLFVKLLGLRLPAGPWGF"
     gene            complement(1575..1964)
                     /locus_tag="VFY80_01370"
     CDS             complement(1575..1964)
                     /locus_tag="VFY80_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6006579.1"
                     /translation="MSRIVHLALKVEDLDRTTEFYQTVFGFREVETRKTRDHLSRHLT
                     DGALDFTLIKYDEGADSAESKASGEGPCIHHFAIEVEDIAKSAAQLRQYGCEIISDPG
                     VIPVKFRAPGGTVAELVPVGRYKKSAN"
BASE COUNT          452 a          696 c          683 g          334 t
ORIGIN      
        1 tggacgcagt ccccacatga cgagcgcaac cagcaggatc gcagtcgtgc ccgaacccgg
       61 aatgccgagc gtgagcagcg gcacgagcgc gccaccggtt tcggaattgt tggcggcttc
      121 cggcgcagcg acgccttcca ccgcaccggt gccgaacttc tcgggatggc gcgataccgc
      181 cttctcgata ccgtaggaca cgaaggaagc gatcgtcgag ccggcgccgg gcagcacgcc
      241 gacgaagaac ccgacgagcg agccgttgag gaaggcgaag cgactgtcct ttaaatcctg
      301 gaggttgggc agcaaattac gcaatccctt cggcacattg aagagctgcc cgccgcccgt
      361 gctcgtcagg ttggcgagca cctcgccgat ggcgaaggtg ccgattgcga cgacgacgaa
      421 gttgacgccg tccacgagct cgacacgatc gaaagtgaag cgcgcgttgt cggtgaaaag
      481 atcgacgccg acagtcgcga gcagcaggcc aaggaacatc gacagcagcg ccttgatcat
      541 cgacttgcct gcaagtagca ccaccatcgc gagtcccatc gccatgaggg cgaagtattc
      601 cggcggcccg aaccgcagcg cgaattccgc cacgggcggc gcgaccagca tcaagagcag
      661 cacgccgatg gtgcccgcga cgaaggatga aatcgccgcg atgccgagcg ccggcccggc
      721 ccgaccccgg cgcgccatct cgtagccgtc caggcaggtg acgacggaag ccgattcacc
      781 aggaacgttc agcaggatgg acgtggtgga cccgccatac ttggcaccgt aatagatgcc
      841 cgccatcatg ataaggcccg acgtgggatt catctgcatg gcgagcggca gcagcagcgc
      901 gacggtggct gacggcccga gccccggaag aatgccgacg acagttccga agaacacacc
      961 gacgaagcac cagaagagat tcatcggctc gagcgccacc gcgaatccgt gcatgagtcc
     1021 ggcgagcatt tccatggcga gcccggtcta aaaaccccac ggcccggcgg gaagccgcaa
     1081 gccgagcaac ttcacgaaga gaaaccagaa gctcagcgca catgcggcag ccgtggcgat
     1141 cgtggtgatg tacgaccgtc ggtagatgac acgcacgtag atcacgacga ggacgatgag
     1201 cgcagcgatg aagccgatcg gcttgatcag cgcaacgact accatgaacg ctgcccagcc
     1261 ggcgatgacg agggcggttc ccgaccagcc cgttcgatgc accggctgcc cgcgcatcac
     1321 gtcgtacgcg tgactcgcga ggtagagcgc ggagagcgtg accatcgcaa tgccgatcca
     1381 gagcgggaag aaacccggtc ccggcccgtc atgggtcatg tactcccatg cgcgcgcctc
     1441 gtgaatcgcg aagagcccga gcgcgatgag cgtaacgctc gcgacgagtt cactcctgtc
     1501 gatcgatttc ctgggactca ctcctgcccc ggcgatccgc cggtcctcct ctctcgcgac
     1561 gcgccgcgcg gaagtcagtt ggcgcttttc ttgtagcggc cgaccgggac gagctcggcg
     1621 accgtcccgc cgggcgcacg gaatttaacc ggaatcactc cgggatcgct gatgatctcg
     1681 cagccgtact gcctcagctg cgccgcggac ttcgcgatgt cctccacttc gatcgcgaaa
     1741 tgatggatgc agggtccctc gcccgaggcc ttggactcgg cggaatcggc accctcgtcg
     1801 tacttgatga gcgtgaaatc gagcgcccca tcggtcagat ggcgcgacag atgatcgcgc
     1861 gtcttgcgcg tttcgacctc gcgaaagccg aataccgtct ggtaaaactc cgtggtccgg
     1921 tcgagatcct cgaccttcag ggcaaggtga acgatgcggc tcatgtctca gctcttcggg
     1981 taaatgaggt agcggaatca gctctccact tcgaaacctg ccttggccag gatgggtcgg
     2041 ctcgcgggcg cggtgaggcg cgacagaaaa agctgcgccg cctcgggttc ctgcgcctgc
     2101 acggtcaccg ctgcagaata aacggtcttc gcctgcagac cttgcggtaa cggcccggca
     2161 agtgt
//
LOCUS       DASURG010000081         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_834456, whole genome shotgun sequence.
ACCESSION   DASURG010000081 DASURG010000000
VERSION     DASURG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834456"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VFY80_01375"
     CDS             complement(<1..1323)
                     /locus_tag="VFY80_01375"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6006580.1"
                     /translation="MRVSRFFISTLKEAPSDAEVVSHRLMLRAGLIKRLTSGVYTWMP
                     LGLRVLRRVEAIIREEMNASGAVELLMPAVQPAELWQESGRWAEYGPELLRMKDRHQR
                     DYCFGPTHEEVVSDVVRREVKSYRQLPLNLYQIQTKFRDEIRPRFGVMRGREFIMKDA
                     YSFHAHKASADATYREMYDCYSRIFTRLGLKFRAVAADTGAIGGTGSHEFHVLADSGE
                     DAIAFCPSSDYAANVELAEALAPARPRAAPSQHLTRVHTPGVKTIAELCAFLRIPVTQ
                     TVKAVCIDGADGRPVLMMVRGDHQLNLIKAGKLPQVKRPIGFSSAAAIREAFGADPGS
                     LGPVGFKGTMIADRTVGAMADFVVGANEDDYHYTGANIGRDFPEPVYADLRNVVEGDP
                     SPDGKGVLDICRGIEVGHIFQLRTKYSSTMNLTFLDESGKPQLMEMGCY"
     gene            1383..2171
                     /locus_tag="VFY80_01380"
     CDS             1383..2171
                     /locus_tag="VFY80_01380"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX6006581.1"
                     /translation="MKFLAKWRLRKPADDAETVYVSEKYGVRSLHIGSDTVQSAMRIA
                     KPNDLELSYTRSMMAFLLFSDRPTRILMVGLGGGSLAKFVYHRLPWVTTEVIEVNPRV
                     VAIARQCFHVPQDEARIKVIVGNGADYVSEPGRIFDVIAVDGYDGESQVEELSDKAFY
                     LACRARLTAGGMVVVNLWGSDRKFQETLVKIEAAFPSGTLCLPAEKPGNIIVFGFRQC
                     PPAWRWDALAARARELETRYELEFPRFVAGLRKMNRHDADQLYA"
BASE COUNT          422 a          724 c          743 g          433 t
ORIGIN      
        1 gtagcagccc atctccatca gctgaggctt tcccgactcg tcgaggaacg tcaaattcat
       61 ggtactggag tacttggtgc gcagctgaaa gatgtggccc acctcgatgc cgcggcaaat
      121 gtcgagcacg cccttgccgt ccgggcttgg gtcgccctcg accacattgc ggaggtcggc
      181 gtagaccggt tccggaaagt cccggccgat gttggcgccg gtgtagtggt agtcgtcctc
      241 gtttgcgccg acgacgaaat ccgccattgc gccgaccgta cggtcggcga tcatcgtgcc
      301 cttgaagccc accgggccga gcgagccggg gtcggcgccg aacgcttcgc gtatcgcagc
      361 ggcggaggaa aagccgatcg gccttttcac ctgaggaagc ttcccggcct tgatgagatt
      421 gagctgatgg tcgccgcgca ccatcatgag caccggacgc ccgtccgcgc catcgatgca
      481 gacggctttc acggtctggg tgacgggaat cctgagaaag gcgcaaagct ccgcgatggt
      541 cttcacgccg ggcgtgtgaa cccgcgtgag gtgctgcgaa ggcgcggcgc gcgggcgtgc
      601 aggcgcgagc gcttcggcga gctcgacgtt cgccgcgtaa tcggagctcg ggcagaacgc
      661 gatcgcatcc tctccggaat ccgccagcac gtggaactca tgcgaaccgg tgccgccgat
      721 cgcgccggtg tccgctgcga ctgcgcggaa tttaagccca agccgcgtga agatgcgcga
      781 atagcagtcg tacatctcgc gataggtcgc atcggcgctc gctttgtgcg catggaagga
      841 gtaagcgtcc ttcatgatga actcgcgtcc gcgcatgacg ccgaatcgcg gccggatctc
      901 gtcgcgaaac ttggtctgga tctgatagag attgaggggc agctgccgat agctcttcac
      961 ctcgcgccgc acgacgtcgc tcaccacttc ctcgtgcgtt ggcccgaagc agtagtcgcg
     1021 ctgatggcga tccttcatgc gcaggagctc cggaccgtat tccgcccagc gtccggactc
     1081 ctgccacagc tcggccggct gcacggcggg catgaggagc tcgacggcgc cgctcgcgtt
     1141 catctcttcg cggatgatcg cctcgacccg gcgcagcacg cgcagtccca gcggcatcca
     1201 ggtgtacacg ccgctcgtga gccgcttgat gagtcccgcg cgcagcatca gcctgtggct
     1261 caccacctcg gcgtcggagg gcgcctcctt gagcgtcgag atgaagaatc gcgagacccg
     1321 catggctgtt ccgcaagcaa aaaagccatt attctaccgg acctcgaaat tcccgacgca
     1381 tcatgaaatt ccttgcgaaa tggcgcctgc gcaagcctgc ggacgacgcc gagaccgtgt
     1441 atgtgagcga gaagtacggc gtgcgctctc tgcacatcgg cagcgacacg gtccagagcg
     1501 cgatgcgcat cgcgaagccg aacgatctcg agctctctta cacgcgcagc atgatggcgt
     1561 ttctgctctt cagcgatcgg ccgacccgca tcctgatggt ggggctgggc ggcggctcgc
     1621 tcgcgaaatt tgtctaccac cggctgccat gggtgacgac cgaagtgatc gaagtgaatc
     1681 cccgcgtggt agcgatcgcg cgccagtgct ttcatgtgcc gcaggacgaa gcgcgcatca
     1741 aggtcatcgt gggcaacggc gccgattacg tcagcgagcc cggtcgcatc ttcgatgtga
     1801 tcgcagtgga cggctacgac ggcgaatcgc aggtcgagga gctgtccgac aaggcgtttt
     1861 atttggcgtg ccgcgcgcgc ctcactgcgg gcggaatggt cgtggtcaac ctctggggaa
     1921 gcgaccgcaa gttccaagag acgctcgtca agatcgaagc cgcttttccc tcgggcacgc
     1981 tgtgtctccc ggcggagaag ccgggcaaca tcatcgtgtt cggctttcgc cagtgccctc
     2041 cggcgtggcg ctgggacgcg cttgcggcgc gcgcgcgcga actggagacc cggtacgaac
     2101 tggaatttcc gcggtttgtc gcgggcttgc gcaagatgaa ccgtcacgac gccgaccagc
     2161 tctacgcgta atccgcatgc gcgtgatccg cgcgctttca ttcctgcgga aattgtggaa
     2221 aaatccttgc caactgacag agttacgggg tattcccatg gtagaccgtg atggatatcg
     2281 ccccaacgtt ggcatcattc tgtgcaactg gaagaacgag gt
//
LOCUS       DASURG010000082         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1448310, whole genome shotgun sequence.
ACCESSION   DASURG010000082 DASURG010000000
VERSION     DASURG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448310"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VFY80_01385"
     CDS             complement(<1..1125)
                     /locus_tag="VFY80_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6006582.1"
                     /translation="MPSWLLNVFPFLRWRASITRETLRADAMAGLVGAAIVLPQGVAF
                     ATLAGMPPQYGLYAAMVPAVVAALFGSSWHLVSGPTNVISIVVFASLSPVAIPGSPEY
                     VRLALTLTFLTGIMQLAMGLARMGILVNFISHTVIIGFTAGAACLILAAQIPNFFGVE
                     ITRGASFPETLSQFAAHLGDINPYVTAVGVITLLSGIAARRYLPRLPYMIVAMVAGTL
                     FALGLNLGLGVEETRIATLGVLPAALPSLSAPDFSPNTLKNTLPVALAVTILGLTEAV
                     SIAKSLAVKSGQRIDANQEFIGQGLSNIFGSFFSAYASSGSFNRSGANQEAGARTPLA
                     AIFSAAMLVIILFAVAPVVAYLPLAVMGAILFMVAYGLFDL"
     gene            1307..1651
                     /locus_tag="VFY80_01390"
     CDS             1307..1651
                     /locus_tag="VFY80_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6006583.1"
                     /translation="MKLSPTKLIAASLLAATCGPLAAAQGNAEAGARKNSMCAGCHGI
                     PGYKTAFPEVYSVPKIGGQHAAYIVKALQAYKSGERSHPSMRAIAATLSDQDMADLAA
                     FYASGQSQQASK"
     gene            1669..1989
                     /locus_tag="VFY80_01395"
     CDS             1669..1989
                     /locus_tag="VFY80_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6006584.1"
                     /translation="MNKLLLAAGCALALSAGQAMAGDAYAGEKKSKPCEACHGVAGNS
                     ASAEFPRLAGQHYDYLVKTLSDYKTGVRKNPIMAPQAANLSRRDIEDLAAFYSKQRGL
                     YVKY"
     gene            complement(1986..2642)
                     /gene="nth"
                     /locus_tag="VFY80_01400"
     CDS             complement(1986..2642)
                     /gene="nth"
                     /locus_tag="VFY80_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6006585.1"
                     /translation="MSPAKVRELFSRLRAQNPAPRGELEFNSPFELLVAVILSAQATD
                     KSVNLATRELFRAANTPQALLSLGQEGLERYIKRIGLYRTKAKNILAACRILADRHGG
                     AVPRNREALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGADVLAVE
                     NKLLKVVPEEFRHDAHHWLILHGRYVCRARKPDCPRCIICDLCQYRPKTRGDGARTPA
                     "
     gene            complement(2694..>3071)
                     /locus_tag="VFY80_01405"
     CDS             complement(2694..>3071)
                     /locus_tag="VFY80_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEX6006586.1"
                     /translation="IEEAACIGCTLCIQACPVDAIVGAAKLMHTVIADECTGCELCLP
                     PCPVDCIVMQPRRIAPTVEQRRHVAAQARRRHEAKTARLARERASRAARPDASRSAAQ
                     ERKRAAVERAIARAQLRLQQRGR"
BASE COUNT          579 a          980 c         1008 g          504 t
ORIGIN      
        1 cagatcgaac agcccgtagg ccaccatgaa caggatggca cccatcaccg cgagcggcag
       61 gtatgccacc acgggcgcta cggcgaacag aatgatcacc agcatcgccg cggagaagat
      121 tgcggcgagc ggcgtgcgtg cgccggcctc ctggttcgcg ccgctgcggt tgaaggatcc
      181 gctgctcgca taagcggaga agaagctgcc gaaaatgttc gaaagaccct gcccgatgaa
      241 ctcctggttc gcatcgatgc gctgaccgga tttgaccgcc agcgatttcg ctatcgaaac
      301 cgcttcggtc agcccgagga tcgtgactgc cagtgcgacg ggcaacgtgt tcttgagcgt
      361 attgggtgaa aaatccggcg ctgacaggga cggcagggcg gcgggcaata cccccagcgt
      421 agcaatgcgg gtctcttcca cgcccagacc gagattgagt cccagtgcga aaagggtccc
      481 tgcgaccatc gccacgatca tgtacggcag gcgcggaagg tagcggcgcg ctgcgatccc
      541 cgagagcagc gtgatgacgc cgaccgccgt cacgtacgga ttgatgtcgc cgaggtgcgc
      601 ggcgaactga ctcaaagttt ccgggaatga tgcgccgcgc gttatctcca cgccgaagaa
      661 gttcggtatc tgggcggcga gaatgaggca ggcggcgccc gcggtgaagc cgatgatcac
      721 cgtgtgcgag atgaagttca ccaggatgcc catccgggcg agccccattg caagctgcat
      781 gatgccggtg aggaacgtga gcgtaagcgc gaggcgtacg tactcggggc tgccgggaat
      841 cgcgaccggg ctcaggctcg cgaagaccac gatggaaatc acgttggtgg gcccggagac
      901 caggtgccag gacgagccga acaatgcagc caccaccgcc ggcaccatgg cggcgtacag
      961 cccgtattgc ggcggcatgc cggccagcgt cgcaaaagcg acgccctgcg ggagcacaat
     1021 ggccgccccg acgagtccgg ccatcgcgtc cgcgcgcagc gtctcccgcg tgatcgacgc
     1081 gcgccagcgc agaaacggaa acacgttgag cagccaggac ggcaagtgag agcgcgattg
     1141 cgtgttcaat ggtatggagc ggtccgctgc aaaatgcatt atcgggcatc atgcacagtg
     1201 cggcaacgcg agagtgcagg ttttccgtgc ctgctcgttg aggcggcgac catgcatcgg
     1261 gtatactagc gcccggaaaa accgcccacg ccatcgcgaa gcaaagatga agctttctcc
     1321 gacgaagttg attgccgcct ccttgctcgc ggcaacttgc ggtccgctcg ccgccgctca
     1381 aggcaacgcc gaggcgggcg ctcggaaaaa ctccatgtgc gcaggctgcc acggcatacc
     1441 cggttacaag accgcgttcc ccgaggtcta cagcgtgccg aagataggcg gtcagcacgc
     1501 tgcctatatc gtcaaggcgc tgcaggcgta caagtccggg gagcgcagcc atccctccat
     1561 gcgcgcgatc gcggcgacgc tttcggacca ggacatggcc gatctagcag cgttctacgc
     1621 gtccgggcag tcgcagcagg cgtccaagtg atcgcggggg catgccggat gaataaattg
     1681 ctactcgctg caggctgcgc gctggcattg agcgccggac aggcgatggc gggcgacgcc
     1741 tacgccgggg aaaagaaatc caaaccgtgc gaagcctgtc acggcgtcgc cggcaacagt
     1801 gcatctgcgg agtttccacg gctcgcgggt cagcactacg actacctcgt caagacgctc
     1861 agcgattaca aaacgggcgt gcgcaagaac cccatcatgg cgccgcaggc ggccaatctc
     1921 tcccggcggg atatcgagga tctcgctgcg ttctattcga agcagcgcgg cctctacgtc
     1981 aagtactagg cgggagtgcg cgcaccgtcg ccgcgggtct tgggacggta ctggcacaga
     2041 tcgcaaatga tgcacctcgg gcagtcgggc ttgcgtgccc ggcacacgta gcgtccgtgc
     2101 aggatgagcc agtggtgcgc atcgtgcctg aactcctcgg gcaccacctt gagcagtttg
     2161 ttctccacgg cgagcacatc cgcgcccggc gcgaggccgg tacggttcgc gaccctgaag
     2221 atatgagtgt ccacggctat cgtcggctcc ccgaacgcgg tgttgagcac gacgttggct
     2281 gtcttgcggc ctacccccgg cagcgcttcg agcgcctccc ggtttctagg caccgcgccg
     2341 ccatgccgat ccgccaggat cctacatgcc gccagtatgt tcttcgcctt ggtgcgatac
     2401 aggccgatgc gcttgatgta acgctccagt ccttcctgcc ccagcgaaag tagcgcctga
     2461 ggcgtattcg ccgcccggaa cagctcgcgc gtggcgagat tgacgctctt gtcggtggcc
     2521 tgtgccgaca ggatgaccgc gacgagcagc tcgaacggcg agttgaactc gagctcgccg
     2581 cgtggcgcgg ggttttgtgc gcgcagacga gagaacagct cccggacttt ggcggggctc
     2641 atgggctagc gtcttctggc gatcatcgcc cgatggccag agcgcatcgg cgctcagcgc
     2701 ccacgctgct gcagccgcag ctgtgcgcgc gcaatcgctc tttccaccgc cgcgcgcttg
     2761 cgctcctggg cggcgctccg cgaggcatcc ggtcgcgcag cacgcgatgc ccgttcgcga
     2821 gcgagacgcg cggttttcgc ttcatggcgc cgccgcgctt gagcggcgac atgtcggcgc
     2881 tgttcgaccg tgggcgcgat ccgccggggc tgcatgacga tgcagtccac cggacatggc
     2941 ggcaggcaca gctcgcaacc ggtgcactcg tccgcgatga cggtgtgcat gagcttcgcc
     3001 gcgccaacga tcgcgtctac cgggcacgcc tgtatgcaca gcgtacaacc gatacaggcg
     3061 gcttcctcga t
//
LOCUS       DASURG010000083         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1243612, whole genome shotgun sequence.
ACCESSION   DASURG010000083 DASURG010000000
VERSION     DASURG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243612"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFY80_01410"
     CDS             <1..572
                     /locus_tag="VFY80_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX6006587.1"
                     /translation="AELIVQGSHELAALMASGDDHEKRAYNIESIEKRADRVTRSTIE
                     LLHKSFITPLDRDDIHQLITKMDDILDLLEDSAQLMFLYGVRRTTEEGRRLAEICVAC
                     CEKVRSTVALLSNMKNAAQIMALCGDMDRLESEADHVMRAAIARLFREEPDVRELIKL
                     RSVYEHLETVTDRCEDVANIIQGIVLENS"
     gene            613..1605
                     /locus_tag="VFY80_01415"
     CDS             613..1605
                     /locus_tag="VFY80_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX6006588.1"
                     /translation="MSFEVLVFLIVVALAFDFMNGFHDAANSIATVVSTRVLKPHQAV
                     LLAAAFNFAALFVFHLKVATTVGKGIIDPQIVDHYVVFGALSGAIAWNLITWYYGIPS
                     SSSHALIGGLIGAALAKAGGWALVPSGILKTVAFIFISPMVGFLLGALLMLLVSMLFM
                     RTTPRRIDRWFRRLQLVSASLYSLGHGGNDAQKTAGIIWMLLIAAGSLRPTDPIPIWV
                     VVACYVTIAFGTAFGGWRIVKTMGQRITKLKPVGGFCAETGGAITLFVATALGIPVST
                     THTITGAIVGVGSMHKFSAVRWGIAGDIVWAWILTIPCSAFMAAVGWWVATHFL"
     gene            1708..2988
                     /gene="egtB"
                     /locus_tag="VFY80_01420"
     CDS             1708..2988
                     /gene="egtB"
                     /locus_tag="VFY80_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX6006589.1"
                     /translation="MPSAAPASTAESVVHELASRFRDVRAVTERLAAPLSAEDCALQS
                     MPDASPTKWHLAHTSWFFETFVLERGLPHFAYFHPRFRTLFNSYYVGVGARHPRPERG
                     LLSRPSLDEVLAYRRHVDRAMERLFTAGLDRNLADIVELGIHHEQQHQELILTDLKHL
                     LSRNPLHPAYLTLSPAREASTTRALDWIAHPAGLRSIGHDAAGFCFDNETPRHRAYVA
                     AFEIADRPVTNGEYLAFIEDKGYARPELWLAEGWDTRAAHDWQAPLYWLQDAGDWFSF
                     TVRGLQPLDPAEPVAHVSYYEADAYARWTGARLPTEHEWETLATSCPVDGHFLESGRW
                     HPRATLSERDGAVQVYGDVWEWTGSAYSPYPGYRPMAGAIGEYNGKFMVNQYVLRGGS
                     CATPRSHIRATYRNFFPAAARWQFSGIRLARDRE"
     gene            complement(2992..3672)
                     /locus_tag="VFY80_01425"
     CDS             complement(2992..3672)
                     /locus_tag="VFY80_01425"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX6006590.1"
                     /translation="MNPMTRLLVAAALFLATHYVSSTPLRARLIRGLGTNGYLLLYSA
                     IAFVTLAAMVWAYYRAPYIGLWHAPLLRYVPLVVMPAAFILMACGLLTPNPTMVGADR
                     LLHSAEPARGILRVTRHPLMWGIALWGAAHLLARGDVAALVFFGSFTVLAMSGTALID
                     RRKRAASGELWQRYARVTSNIPFAAIAAGRNRFSAREIGWSRPAAGLALYAVGLWLHP
                     ILFGARPY"
     gene            complement(3721..>4051)
                     /locus_tag="VFY80_01430"
     CDS             complement(3721..>4051)
                     /locus_tag="VFY80_01430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX6006591.1"
                     /translation="GGTVYRYGGEEFSVVFPGKSVEEALPHLEALRKDVAAYRLSLRA
                     PGREEASPPGKKRRGTHPEARAVSVTVSIGVAECSAAAATPEEVIHAADRALYRAKNR
                     GRNQVSR"
BASE COUNT          725 a         1279 c         1294 g          753 t
ORIGIN      
        1 acgccgaact catcgtgcag ggcagtcacg agcttgcggc attgatggcg agcggcgacg
       61 accacgagaa gcgcgcctac aacatcgagt ccatcgaaaa gcgcgcggac agggtcactc
      121 gcagcaccat agaactgctg cacaagtcgt tcatcacgcc gctcgaccgt gacgacatac
      181 accagctcat caccaagatg gacgacatcc tcgatctgct ggaggattca gcccagctca
      241 tgtttctcta cggcgtgcgc cgcacgaccg aggaggggcg gcggctggcc gagatctgtg
      301 tcgcctgttg cgaaaaggtg aggagcaccg tcgccctgct ctcgaacatg aagaacgccg
      361 cgcagatcat ggcgctttgc ggcgacatgg atcgcctgga atcggaagcg gatcacgtga
      421 tgcgcgccgc gatagcgcgt ctgttccggg aagagccaga cgtgcgcgag ctgatcaagc
      481 tgcgctcggt ctacgagcat ctcgaaaccg tgacggaccg ctgcgaagac gtcgccaaca
      541 tcattcaggg gatcgtgctg gagaattcct gatccgcccg gcccaacaac aacaaatcca
      601 ggcagcgcgc gcatgagctt cgaggtgctg gtgttcctga tcgtcgtcgc gctcgcgttc
      661 gacttcatga acggcttcca cgacgcggcc aattcgattg cgaccgtcgt gtccacccgc
      721 gtgctgaagc ctcatcaggc cgtgctactc gcagccgcct tcaattttgc cgcgctgttc
      781 gtatttcatc tcaaggtggc gacgaccgta ggcaagggga tcatcgaccc gcagatcgtc
      841 gatcactacg ttgtattcgg tgcgctctcc ggcgcaatcg cgtggaacct gattacgtgg
      901 tactacggca ttccatcgag ttcctcgcat gcgttgatcg gcgggctgat tggcgcggcg
      961 ctcgccaagg cgggtggctg ggccctcgtg ccgagcggca ttctcaagac cgtggcgttt
     1021 atcttcattt caccgatggt aggtttccta ctgggcgcgc tgctcatgct cttggtatcc
     1081 atgctcttca tgcgcacgac gccccggcgg atcgatcgat ggttccgcag gctgcagctc
     1141 gtgtcggcct cgctctacag cctgggtcac ggcgggaacg acgcgcagaa gacagcgggc
     1201 atcatctgga tgcttctcat cgccgccgga tccttgcgcc cgaccgatcc cattccgata
     1261 tgggtcgtcg tggcctgtta tgtcacgatt gcgttcggca ccgcattcgg cggctggcgc
     1321 atcgtcaaga cgatgggcca gcgcattacc aagctcaagc cggtgggtgg attctgcgcg
     1381 gagacgggtg gtgcgataac gctgttcgtc gcaacggcgc tgggaattcc ggtctcgacc
     1441 acccatacca tcaccggggc catcgtgggc gtcggttcga tgcacaagtt ctcggcggtg
     1501 cgctggggga tcgcgggcga catcgtgtgg gcgtggatac tgacgattcc ctgctccgcg
     1561 ttcatggcgg ctgtcggctg gtgggttgcc acgcacttcc tgtagtcatt cgttcggcag
     1621 cgcggaacaa caacggcggc tacgcggtca gcgtaagact gaaccgttcg attagaggag
     1681 ggcctcagca tgactttacg gcccggcatg ccgtcggcgg caccggcttc gacggcggaa
     1741 tcggtcgtgc acgaactggc gtcccgcttc cgcgatgtgc gcgccgtgac cgaacggctc
     1801 gcggccccgc tatccgctga ggactgtgcg ctgcagtcca tgccggatgc tagccccacc
     1861 aagtggcatc ttgcgcatac gagctggttt ttcgagacgt tcgtgctgga gcgtggattg
     1921 cctcatttcg cttacttcca tccgcggttt cggacgcttt tcaattccta ttacgtaggc
     1981 gtgggcgcac gccatcccag accagagcgc ggcctgctgt cgaggccgtc gctcgacgaa
     2041 gtgcttgcct atcgccggca cgtggaccgg gccatggagc gactctttac ggcgggactc
     2101 gatcggaatc tcgccgacat cgtggagctc ggcattcatc atgagcagca gcatcaggag
     2161 ctgatcctca ctgacctgaa gcacctgctt tcgcgcaatc cgctgcatcc ggcgtacctg
     2221 acgctttctc cggcacggga agcttccaca acgcgcgcac tcgactggat cgcacatccg
     2281 gccgggctgc gatcgatcgg acacgatgca gccggtttct gcttcgacaa cgagacgccc
     2341 cgccaccgcg cgtatgtcgc agcgttcgag atcgccgacc gaccggtaac gaacggcgag
     2401 tatctcgcgt tcatcgagga caaaggctac gcgcggcccg agctgtggct cgcagagggt
     2461 tgggacacgc gggctgctca cgactggcaa gcgccgctct actggctgca ggatgccggt
     2521 gattggttct ctttcacggt gcgcgggctc cagccgctcg acccagcgga gcccgtggct
     2581 catgtgagct actacgaagc ggatgcctat gcgcgctgga ccggcgcacg gctgccgacc
     2641 gagcatgagt gggaaacttt ggcgacctct tgcccggtgg acggtcactt cctcgaatcc
     2701 ggtcgctggc atccgcgcgc cactcttagc gagcgtgacg gcgcggtgca ggtgtacgga
     2761 gacgtttggg aatggacggg cagcgcctat tcgccctatc ctggataccg ccccatggcc
     2821 ggtgcaatcg gcgagtacaa tggcaagttc atggtgaatc agtacgtcct gcgcggagga
     2881 tcctgtgcga cgccgcgctc acacatccgc gccacctacc gcaatttctt tcccgctgcg
     2941 gcgcgctggc agttcagcgg catccggctc gcgcgcgacc gcgagtgatc gttaataggg
     3001 gcgcgcgccg aaaagaatcg gatgcagcca aaggccgacg gcgtacagcg cgagtccggc
     3061 ggcgggtcgt gaccagccga tttcacgtgc gctgaagcga ttgcgcccag ccgctatggc
     3121 ggcaaagggg atattcgacg tgactcgcgc atagcgctgc cagagctcac cgctggccgc
     3181 gcgcttgcgc ctgtcgatga gagcggtgcc gctcatggcg agcactgtga aactaccgaa
     3241 gaatacgagc gctgcaacat cgccgcgtgc gagaaggtgc gccgcgcccc agagggcaat
     3301 tccccacatc agcgggtggc gcgtcacgcg caggatgccg cgcgccggct ccgcggagtg
     3361 tagcaggcgg tcggcaccca ccatggtcgg attgggcgtg agcagcccgc acgccatgag
     3421 aatgaatgcg gccggcatga cgacgagggg cacatagcgc agaagtggcg catgccagag
     3481 cccgatgtac ggcgcgcgat agtaggccca taccatcgcg gcgagtgtaa cgaacgcgat
     3541 cgcgctgtag agcaggagat agccgttggt gccgagtcca cggatgagcc gcgcgcgcag
     3601 cggcgtgctc gatacataat gcgtcgcaag aaacagcgcg gctgcgacga gcagccgtgt
     3661 catcgggttc atctgcgctt tacttcgtcg ccgtatggac caccattgta gcgaggaaca
     3721 ctagcggctc acctgattgc gcccgcgatt cttggcgcga tacagcgctc ggtccgccgc
     3781 atggatgacc tcttcgggcg ttgcggcggc tgcactgcat tccgcaacgc cgatactgac
     3841 cgtcacggaa accgcacggg cctcaggatg cgtgccgcgg cgctttttcc cgggcggcga
     3901 cgcctcttca cgccccgggg cacggagaga gagccggtag gccgcaacat ccttgcgcaa
     3961 tgcctcgagg tggggcagcg cttcctccac cgacttcccc ggaaagacga cgctgaactc
     4021 ctcgccgccg tagcggtaca cggtcccgcc g
//
LOCUS       DASURG010000084         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_18853, whole genome shotgun sequence.
ACCESSION   DASURG010000084 DASURG010000000
VERSION     DASURG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18853"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..909)
                     /locus_tag="VFY80_01435"
     CDS             complement(307..909)
                     /locus_tag="VFY80_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEX6006592.1"
                     /translation="MPTAPRPQPIALRIELLDVAPLVWRRVLVSNQWTLASLHGYLQW
                     VMGWTDSHAHEFQIGAGIVAPDWWIREADFDTETRDCRDERRVSVAAVVSELGAAGEF
                     EYRYDMGDGWQHRLVIESLPRVAETQDLSLPVCLAGENACPPEDVGGPGGYELFLEIL
                     ADRAHEQHHDMLRWIGGVFDPKGFDLNRVNRDFRARKRRR"
     gene            complement(1449..>2065)
                     /locus_tag="VFY80_01440"
     CDS             complement(1449..>2065)
                     /locus_tag="VFY80_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6006593.1"
                     /translation="VPAREEELRKGVAQYAGFSLHAGIGVEADEREKLERLARYVSRP
                     PVSMDRLSLTAQGQVRYRLKTPYRDGTTDIVLEPLDFIARLAALVPPPRVHLTRYHGV
                     FAANAILRAAITPAGRGSKARSRPLERRPPKHVALNWARRLKRVFGIEIEQCARCGGR
                     LEVIASIEEPELIERILAHRRETEGDEGLTVSLGARAPPQASLF"
BASE COUNT          394 a          692 c          618 g          361 t
ORIGIN      
        1 acgaggtaat agcgaccgtt cattcagatt gccctccgct gcgcggacgg atggcctcga
       61 aagtgctgac acccttcgag gccaaggtgt tacccatgtc ttcggtatga gatggtggcc
      121 agggacggaa tcgaagagaa cgggcaactt gctgatttag cggtcaaatc ctagttctga
      181 aaatcgctgt taccacgaag gttacccagg gatttttctc gtttttttta gagcccgaac
      241 tacgggtcgc ggaacctatc tgcgcgcgct tcgcacacac tttgcgaaca ttctccgccg
      301 cgccgatcaa cgccggcgct tcctcgcgcg gaaatcccgg ttgactctgt tcaagtcgaa
      361 gcccttcgga tcgaacacgc caccgatcca ccgcaacatg tcgtggtgct gctcgtgcgc
      421 gcgatcggca agaatctcga ggaacagctc atagccaccc ggaccaccga catcttcggg
      481 tggacaggca ttttcgccgg caagacatac cggcagcgac agatcctgag tctccgcaac
      541 gcgtggcagc gactcgatca caagccgatg ctgccatccg tcacccatgt cgtagcgata
      601 ttcgaactcg ccagcggcgc ccagctccga aacgacggca gcgacggaga cgcggcgctc
      661 atctcgacag tcacgggttt cggtgtcgaa gtcggcttcc cgaatccacc agtccggcgc
      721 gacgattccg gccccgatct ggaattcgtg cgcatgcgaa tccgtccacc ccatcaccca
      781 ctggagatag ccatggagac tggcgagcgt ccactgattg gacaccagca cgcgacgcca
      841 aacgagtggc gctacgtcga gtaactcgat gcgcagagcg atgggctgtg gccgtggggc
      901 agtcggcacg tcggacaact ccaatgcaac ggatggaatg agaacacgag ggcgcgcccg
      961 atggcgagca gcaggcgtcg cctactcgac ttgcggccga ctcgcaacgt cacttgcacg
     1021 agtcccatcg ataacccgaa cgtgtataga aacggtcgcg gctgactttc ggctatcgcc
     1081 atcatcttcg gcgcgagggc gatgacccga tcgaacgcga taccggccgc tgtcagcttc
     1141 gcaacaaggg ccttcgagag gatcgactcg tcgatgtgga agatcggcgc tgcggaggcg
     1201 ggcggagctc gtcagggcag cgggcggata ggtgttttga acacgtgctt ccgcgccggc
     1261 ggcaagagcg cgtaatggga atcgtgtcaa agcctctgcc tgaggcagcg cggaatcggc
     1321 cgcgcgaccc acttcccagc gaatttcggc gcgtcggcgc cgcggatccg ggcctgtgaa
     1381 agcaccgcaa gcagcgccgc gcccgctcgc gctcgagtaa gcgcaagccc gtcgacgatc
     1441 gccccgaatc agaataacga cgcctgcggc ggcgcccggg ctccgagtga gaccgtcagc
     1501 ccctcgtccc cttccgtctc ccgccggtgc gcgagaatgc gttcgatgag ctccggctcc
     1561 tcgatgctcg cgatcacttc gaggcgccca ccacagcgcg cgcactgctc gatctcgatc
     1621 ccgaacacgc gcttcaagcg ccgtgcccag ttcaacgcca cgtgcttcgg cggccgtcgc
     1681 tcgagcggtc ggctgcgcgc cttagagcct cgccccgccg gcgtgatcgc ggcgcgcagt
     1741 atcgcattgg cggcgaacac cccgtgatat ctcgtcagat gcacgcgtgg cggcggcacc
     1801 aacgccgcta ggcgcgcgat gaagtcgagc ggctccagca cgatgtccgt cgtgccgtcg
     1861 cgatacggcg tcttcaaccg ataacgcacc tgcccttgtg ccgtcagcga taaccgatcc
     1921 atcgacaccg gcggccggct cacgtagcgg gcgagccgtt cgagcttttc ccgctcatcg
     1981 gcttctacac cgatcccggc gtgcagcgag aaccccgcgt actgcgccac acctttcctg
     2041 agctcctctt ctcgcgccgg caccg
//
LOCUS       DASURG010000085         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_528904, whole genome shotgun sequence.
ACCESSION   DASURG010000085 DASURG010000000
VERSION     DASURG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528904"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFY80_01445"
     CDS             complement(<1..938)
                     /locus_tag="VFY80_01445"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6006594.1"
                     /translation="MSDLSTLSAIHAASTQLPVNWYFDPRIYELEKRLLFDAGPGYMG
                     HELMVPNVGDYQTLDWLGHSKALVHGAQGVELLSNVCRHRQAIILKGRGTARNIVCPL
                     HRWTYDLGGKLLGAPHFPHNPCLDLAKSPLTRWNGLLFAGRRDVAHDLALLGGMRDLD
                     FSGFMFDRVEVDEYSCNWKTFIEVYLEDYHVEPFHPGLGNFVDVSDLQWEFGDWYSVQ
                     TCGVKKALAKPGSEVYRRWHEEVLRYGEGRTPRHGAIWLTYFPNIMVEWYPHSLVVST
                     IIPRGPEACSNVVEFYYPEDIVLFEREFVEAEQAAYR"
     gene            complement(1129..1761)
                     /locus_tag="VFY80_01450"
     CDS             complement(1129..1761)
                     /locus_tag="VFY80_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6006595.1"
                     /translation="MPRTNPDARLLVFTKAPRAGAVKTRLAPLLGEEGASSLQRRLIV
                     HTLATARRAAIGALELHAAPDCDDAFLQSCARRYGARLVAQVAGDLGTRMASAMSRAL
                     AESARAIAIGTDCPVLSARHLVAACNALADNEAVLIPTEDGGYALIGLVQHDPRLFRD
                     IDWGTERVMNQTRARLSELGRRWHEMETLWDVDRPEDYRRLEALTLMLES"
     gene            complement(1742..>2233)
                     /locus_tag="VFY80_01455"
     CDS             complement(1742..>2233)
                     /locus_tag="VFY80_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6006596.1"
                     /translation="MNAGAARASGGILLFLHADCRLPLEADRLVIEGLQRTRRSWGRF
                     DVHIESRSPLLRLVAALMNLRSRMTGIATGDQALFVTRSLFETVGGFPAIGLMEDIAL
                     SRRLKRYGRPLCLKHKVAVSARRWERHGILATIVLMWRLRFAYWLGADPDELAMRYAP
                     HKP"
BASE COUNT          410 a          702 c          743 g          378 t
ORIGIN      
        1 cggtaggccg cctgctccgc ctcgacgaat tcgcgctcga acagcacgat gtcctcggga
       61 tagtagaact cgaccacgtt actgcacgcc tcggggccgc gcgggatgat ggtactcacg
      121 acgagcgaat gcggatacca ctccaccatg atgttcggaa aataggtgag ccagatggcg
      181 ccgtgccgcg gcgtgcggcc ctcaccgtaa cgcagtacct cctcgtgcca gcggcgatac
      241 acttcgctac ctggcttggc gagtgccttt ttgacaccgc aggtctgcac gctgtaccag
      301 tcgccgaact cccattggag gtcggagacg tcgacgaagt tgccgaggcc cggatgaaac
      361 ggctcgacgt ggtagtcctc gagatagacc tcgatgaacg ttttccagtt gcaggaatat
      421 tcgtccactt ccacgcgatc gaacatgaag ccggaaaagt cgagatcgcg catcccgccc
      481 agcagcgcga ggtcgtgcgc gacatcgcgg cggccggcga agagcagtcc gttccaccgc
      541 gtgaggggcg acttcgcgag gtcgaggcag ggattgtgcg gaaagtgtgg cgcgcccagg
      601 agctttccgc ccaggtcgta ggtccagcgg tgaagcgggc acacgatatt gcgcgccgtc
      661 ccgcgccctt tgaggatgat cgcctgccgg tggcggcata cgttcgagag gagctcgacc
      721 ccctgcgcgc catgcaccag cgctttcgaa tggcccagcc agtcgagcgt ctggtagtcg
      781 ccgacattcg gcaccatgag ctcgtgcccc atgtacccgg ggcccgcgtc gaaaagcagg
      841 cgcttttcca gctcgtagat gcggggatcg aagtaccagt tgacgggaag ctgcgtcgat
      901 gcagcgtgaa tggcggacag cgtcgaaaga tcggacatgc agaggacgtt cggtcgtatt
      961 tagtgggatc gtcggcggcg tctggaacgc atggaatgca gtaagcccct gaaaaagagg
     1021 cgctattctt cccgaaccgg ttggaaaagg caatcggttt ttgcgcggca acgacgcctg
     1081 ccggcgccgt gcggactaac tgccggttta tgcaggacta atcccttgtc aactttccag
     1141 catgagcgtg agtgcttcga ggcgccggta gtcctccggg cgatccacgt cccagagggt
     1201 ttccatctcg tgccagcgcc gcccaagctc agaaagccgc gcgcgggttt ggttcatcac
     1261 gcgctccgtt ccccaatcga tgtcccggaa gaggcggggg tcgtgctgca caagaccgat
     1321 cagcgcatag ccgccgtctt cagtgggaat gagaaccgcc tcgttgtccg cgagcgcatt
     1381 acaggctgcc acgagatggc gcgcgctcag caccggacag tcggtgccga tggcgatcgc
     1441 gcgagcgctc tcggcgagcg cccggctcat cgcgctcgcc atgcgcgtgc cgagatcgcc
     1501 cgctacctgc gccacgagcc gggcaccgta acgcctggcg caggactgaa ggaacgcatc
     1561 gtcgcagtcg ggcgcagcat gcagttcgag cgcgccgatt gcggcccggc gcgccgttgc
     1621 gagcgtatgg acgatcagcc gtcgctgcag cgacgaggcg ccttcttcgc ccagaagcgg
     1681 ggcgagccgc gtcttgacgg cgcccgctcg cggcgccttg gtgaagacga gaaggcgcgc
     1741 gtcagggttt gtgcggggca tagcgcatgg caagctcgtc cgggtcggcg ccgagccagt
     1801 aggcgaagcg caagcgccac atgagcacta tcgtcgcgag gattccgtgc cgctcccagc
     1861 gccgcgccga gaccgcgacc ttgtgcttca agcacagcgg tcttccatag cgcttgaggc
     1921 gccttgaaag cgcgatgtcc tccatgagcc cgattgcggg aaatccgccc accgtctcga
     1981 agagcgagcg cgtgacgaag agcgcctggt cgccggtcgc gattccggtc atgcgcgagc
     2041 ggagattcat gagcgccgcg accagtcgca gcagcggcga gcggctctcg atatgcacgt
     2101 cgaaccgccc ccagcttcgg cgcgtgcgct gcaggccttc gatcacgagc cggtcggctt
     2161 cgagtgggag acggcagtcg gcatggagaa agagcagtat gccgcctgac gcacgagcgg
     2221 cgccggcgtt cat
//
LOCUS       DASURG010000086         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_834843, whole genome shotgun sequence.
ACCESSION   DASURG010000086 DASURG010000000
VERSION     DASURG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834843"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>2146
                     /locus_tag="VFY80_01460"
     CDS             22..>2146
                     /locus_tag="VFY80_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX6006597.1"
                     /translation="MALLTIDAGVAHVRLRALDAAGVPQYDVLFASNRDFATFFNEQR
                     ESGAFADDSRTPVCITGKLAPTVRRALGRGKTFMPAALFWLVASDARAARHAASLALV
                     EISASGYTVIGVDGEGRLKDDLLVVNPRCGAGSGVNVDRVLQKLGLRRDEVDTLLADY
                     LGEGGRSLREQVLTRADRCGVFSSSATISDKNQGIPLPVALATTLKSELLKTCHKVPQ
                     GFEAVVLCGRFFRWQFVRDCAEDYFASIGVHRVEYDAENTLALDTMARLVRTEGSERI
                     AQPDARLISEAPLASYPAFAELHAQYLADRRYRRAESAHPDASEFIAPGDPVHVGLDV
                     GSTMAKICIADAAGEPLHLSAYGNAGDTIETVKTIFRDLQAMGFADLAVRSIGITGSA
                     RYQVKEALAHIYPGLSPRINVLVENYAHARGSIDEARRHLAELKRCGVGALNEDFCVL
                     VDVGGEDTKISTIALKEAELFNNAMNIKCSAGTGSLIDTLAALFGLAGPADASKLGYA
                     APRALAINATCAVFLMENAQKLQAQGVPRGEIIASASWAVVENMARTLWSQVDLPPNC
                     VVLLHGQTMLSDPLPLAITHRLQSFLGQPAYALVPPYPGHRACLGLIRTAVQASVDGV
                     ETIALRDFVTARFEKRLVQCKGAACDDADAVCNRTALKCQGADGRQFSFTLGGCSAIN
                     ELFARSKSRHGETRASVGSDAYKEIW"
BASE COUNT          365 a          682 c          711 g          388 t
ORIGIN      
        1 aggcaggctt cccctaccgc ggtggcctta ttgaccatcg acgccggtgt ggcacacgtc
       61 aggctgcgcg ccctcgatgc cgcgggcgtg ccacagtacg acgtgctctt cgcgagcaat
      121 cgggattttg cgactttctt caacgagcag cgcgaaagcg gagcgttcgc cgacgattcg
      181 cgcacgccgg tctgcattac cggcaaactg gcgcccacgg tgcgtcgggc cctcggtcgt
      241 ggaaagacgt ttatgcccgc ggcgctgttc tggcttgtgg ccagcgatgc gcgcgctgcg
      301 cggcatgcgg cgtcccttgc gctggtggag atctccgcct ccggctatac cgtgatcggc
      361 gtagacggtg aagggcggct caaggatgac ctgctggtgg tcaatccgcg atgcggggcc
      421 ggtagcggcg tcaacgtcga tcgcgtgctg cagaagctcg gtctgcgtcg cgatgaggtc
      481 gacacgctcc ttgccgacta tctgggcgaa ggtggccggt cgctgcgcga gcaggtcctc
      541 acgcgggcgg accgttgcgg cgtattcagc tcctcggcga cgatctccga caagaaccag
      601 ggcattcctt tgcccgtcgc gctggcgaca acgctcaagt ccgaattgct caagacctgc
      661 cacaaggtac cgcagggctt cgaggcggtc gtgctctgcg gccgcttctt ccgctggcaa
      721 ttcgtgcgcg actgcgccga ggactacttc gcgagcatcg gcgtgcaccg ggtcgagtac
      781 gacgccgaga acaccctggc gctcgatacc atggcgcggc tcgtcagaac agagggcagc
      841 gagcgcattg ctcaacccga tgccaggctg atttccgagg cgccgctcgc ctcctatccg
      901 gcgttcgcgg aactgcacgc gcagtatctt gcggaccggc gctataggcg cgccgaatcg
      961 gcacatcccg acgcgtcgga attcatagcc ccgggcgatc cggtgcacgt cgggctcgac
     1021 gtgggctcga ccatggcgaa gatctgcatc gccgatgctg cgggcgaacc gctgcacctg
     1081 tccgcctatg gcaatgccgg tgacaccatc gaaacggtca agacgatctt tcgtgacctg
     1141 caggctatgg ggttcgccga tctcgctgtg cgaagcatcg gcatcacagg ctcggcgcgc
     1201 taccaggtga aggaggcgct cgcccacatt tatcccggcc tgtctccgcg gatcaacgtg
     1261 ctggtggaga actatgccca tgcccgcggt tcgatcgacg aggcgcggag gcatctcgca
     1321 gagctgaagc gatgcggcgt cggcgcgctg aacgaggact tctgcgtgct cgtcgacgtc
     1381 ggcggcgaag ataccaagat cagcaccatc gcattgaagg aagcggagct cttcaataac
     1441 gcgatgaaca tcaagtgctc cgccggaacc ggtagcctca tcgatacgct cgctgcgctt
     1501 ttcgggctcg ccgggccggc cgatgcaagc aagctgggct atgcggcgcc ccgggcgctt
     1561 gccatcaacg ccacctgcgc ggtgtttctc atggagaatg cccagaagct gcaggcgcag
     1621 ggcgtgccga gaggcgagat tatcgcctcc gccagctggg ccgtggtcga gaacatggcg
     1681 cgcacgctgt ggtcgcaggt cgatctgccg cccaattgcg tggtcctgct gcatgggcag
     1741 accatgctgt cggatccttt gccgctcgcc atcacccatc ggctgcagag ctttctggga
     1801 cagccggctt acgcgctcgt gccgccctac ccggggcatc gggcctgctt ggggctgatc
     1861 cgcaccgccg tacaggcgtc ggtcgacgga gttgaaacga ttgcgctccg cgacttcgtg
     1921 acggcccgct tcgagaagcg gctggttcag tgcaaaggcg cagcatgtga tgacgccgac
     1981 gcggtatgca accgcaccgc gctcaagtgc cagggcgctg acggcagaca gttcagcttt
     2041 accctgggcg gatgcagtgc gatcaacgag ctctttgccc gcagcaagtc gcggcatggc
     2101 gagacacgtg cgagcgtggg atccgatgcc tacaaggaga tatggg
//
LOCUS       DASURG010000087         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_937835, whole genome shotgun sequence.
ACCESSION   DASURG010000087 DASURG010000000
VERSION     DASURG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937835"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFY80_01465"
     CDS             <1..406
                     /locus_tag="VFY80_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6006598.1"
                     /translation="YAPRAACFEKRIKACVALSGAYERSSSFEGRPIINVEAFRVRSK
                     SANLEGAGKVAARMTLKGIARNITCPIYIVAGTRDRLTPHTHAEKLAAEVSGPCELSI
                     IEGGNHVVNNLWYRYRDQTADWVATQLGLPKR"
     gene            468..1829
                     /locus_tag="VFY80_01470"
     CDS             468..1829
                     /locus_tag="VFY80_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6006599.1"
                     /translation="MPAKRKNKVEPKAVRRTDIDPKRAVSSAPDGLVDPADPGHRWDR
                     PLQAPGRMQVDFEERIDFRRLHDYRLARTRNALARSGLGALLVFDQYNIRYISSTVIG
                     EWARDKLTRWCLLTGNGEPWVWDFGSAARHHKIYAPWLPKNHCLAGLAGLRGAVSPKV
                     GLFEDAAKEIRDILEQEGVADMPLGIDVVEPPFLFALQKLGIEVRDGQQVMLDAREIK
                     SHDEITLLNMAASMGDGVYQDIFEALKPGVRESEIVALATKRLYEMGSDCVEAINAIA
                     GERCSPHPHNFTDRMIRPGDQAYFDIIQTYMGYKTCYYRTFTVGSATTAQIEAYRKAR
                     DWMDSAIDALKPGVSTDRVVQAFPKAQEIGFETEMAAFGLNFCHGLGLGLHERPLISR
                     LNSFKDSYELKAGMVFAVETFCPAADGISAARIEEEVVLTPDGAKVITLFPAQELPIA
                     NKY"
BASE COUNT          406 a          630 c          648 g          332 t
ORIGIN      
        1 ttacgcgccg cgcgccgcgt gcttcgagaa gcgcatcaag gcctgtgtcg cgctgtccgg
       61 agcgtacgag cgctcgtcga gcttcgaggg tcggccgatc atcaatgtcg aagcgttccg
      121 cgtgcgctcg aagagcgcca acctcgaggg agcgggcaag gtggcggcgc gcatgacgct
      181 gaagggcatc gccaggaaca tcacgtgccc gatctacatc gtcgccggca cccgcgaccg
      241 cctcacgccg cacacgcatg cggaaaaact cgcagccgaa gtgtcggggc cgtgcgagct
      301 ttcgatcatc gagggcggca accacgtcgt gaacaacctg tggtaccgct atcgcgacca
      361 gaccgcggac tgggtggcga cacagctcgg gctgccgaag cgctgatatg ccggtgcgaa
      421 cgggtaatcg cagaggttgc ccgtgtccgt gattctggag aacacaaatg cctgccaaac
      481 gaaaaaacaa ggtggagccg aaagccgtcc gccgcaccga catcgatccc aaacgcgcgg
      541 taagcagcgc gccggacggg ttggtcgatc ctgccgatcc cggacaccgc tgggaccggc
      601 cgctccaggc gccgggccgc atgcaggtcg acttcgagga gcgcatcgat ttccggcgcc
      661 ttcacgacta ccggctcgcc cgcacgcgca atgcgcttgc ccggtcgggc ctcggcgcgc
      721 tgctggtctt cgatcagtac aacatccgct acatttcgag cacggtcatc ggcgagtggg
      781 cgcgcgacaa gctgacccgg tggtgtctgc tcaccggcaa cggcgagccg tgggtctggg
      841 acttcggttc cgcggcgcgc catcacaaga tatacgcgcc gtggctgccg aagaatcatt
      901 gtctcgccgg gcttgcgggc ctgcgcggcg cggtgtcgcc caaagtcggg ctgttcgagg
      961 atgcggcaaa ggagatcagg gacatcctcg aacaggaagg cgtggcggac atgccgctcg
     1021 gcatcgacgt ggtcgagccg cctttccttt tcgcgctgca gaagcttggg atcgaagtgc
     1081 gcgacggcca gcaggtgatg ctggatgcgc gcgagatcaa gagccacgac gagatcacgc
     1141 tgctcaacat ggcggcgtcg atgggcgacg gcgtctacca ggatatcttc gaggcgctca
     1201 agccgggcgt gcgcgaaagc gagattgtcg cgctggcgac gaaacgcctc tatgaaatgg
     1261 gctccgactg cgtggaagcg atcaacgcca tcgccggaga gcgctgcagc ccgcatccgc
     1321 acaacttcac cgaccgcatg attcgtcccg gcgaccaggc ctatttcgac atcatccaga
     1381 cctacatggg ctacaagacc tgctactacc ggaccttcac cgtggggtcg gcgaccacgg
     1441 cgcagatcga ggcctaccgt aaggcccgcg actggatgga cagcgccatc gacgcgctca
     1501 agcccggcgt ttccacggat cgcgtggtac aggcctttcc caaagcgcag gaaatcggct
     1561 tcgaaacaga aatggccgcc ttcggcctca acttctgcca cggactgggt ctcggactgc
     1621 acgagcgccc cctcatctcg cggctcaatt cgttcaagga ttcgtatgag ctcaaagcgg
     1681 gcatggtctt cgccgtcgaa acgttttgcc cggcggcgga cgggatctcg gcggcacgca
     1741 tcgaggagga agtcgtgctg acgccggacg gcgcgaaagt gatcacgctt ttcccggcgc
     1801 aagagttgcc catcgccaac aaatattgaa cttgaaacgc atgaacgcaa aggacgcaaa
     1861 ggacgcgaag gaatccctgg gaatgaatct ttttcctttg cgtccagcgc gtccgcgctt
     1921 tgaaagctga ggtggcatag tggatacgtc cgtcaagcgg aaaaaaacgg ctgcgcccga
     1981 gatcgacatc gggcgggaga cgcggctcga gctcta
//
LOCUS       DASURG010000088         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_938205, whole genome shotgun sequence.
ACCESSION   DASURG010000088 DASURG010000000
VERSION     DASURG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938205"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFY80_01475"
     CDS             complement(<1..697)
                     /locus_tag="VFY80_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX6006600.1"
                     /translation="MVVNEVPVKELLLALARDTKQNIDIHSGLQGLVSLNAINETLPA
                     ILERISRQVDMRYRIEGNTIVVSPDTPYLRTYRVNYVNMTRNTTSTIGVSGELTVTTG
                     GTAATPGASAAAAPAASAGGASGSSTVVRTISNNDFWDQLRDNIRAILAATRARTLNA
                     EEKAKFAEERRTARETALKQAEAVARAGQGATALYAAAFRATPPSPLADIRDDIIVNP
                     VSGTVSVLATERQH"
     gene            complement(1043..>2083)
                     /locus_tag="VFY80_01480"
     CDS             complement(1043..>2083)
                     /locus_tag="VFY80_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006601.1"
                     /translation="EESQSMPLATLEEIRLLSNLETRNEKLLQIVLFGQPELDENLRD
                     GRIRQLKERITHSFRLGPLNTAETRDYLMFRLRAAGYRGPDLFPNRIARYIARASSGL
                     TRRINLIADKALLAAFSENTHTVGLRHVTAAVRDSDFGQDAHPARGLRIVWGAALLLI
                     GALTGALALAWLGESALPVDARKSDRAVHLPSSAGTPANGESPAGMDSPSSASEGRAP
                     IATPAPSAGPDASGALPTLVEQRIAMTREWLAEAPPMTYSIQLMGIQDESQLTLHLND
                     MANFIEVNKVFLYRTVANRKASMTVLYGSFSDRRAAQNALETLPAALKVNRPILRTVR
                     GIRTEIMRHWAS"
BASE COUNT          335 a          629 c          714 g          405 t
ORIGIN      
        1 ggtgctggcg ttcggttgcg aggacactga ccgttccgct tacggggttc acgatgatgt
       61 cgtcgcgaat atccgcaagc ggggagggtg gcgtggcgcg aaacgccgct gcatagagcg
      121 cggttgcccc ctggcctgcg cgcgccacgg cctcggcctg tttgagcgcc gtctcgcgcg
      181 ccgtgcgccg ctcttcggcg aacttcgcct tttcctcggc gttcagggta cgggcgcggg
      241 tggcggcgag aatcgcccga atgttgtcgc gcagctgatc ccagaagtcg ttgttcgata
      301 tcgtccgcac gacggtgctc gagccgctcg ccccaccagc ggacgctgca ggcgccgctg
      361 ccgcggaggc gcctggcgtg gccgccgtgc cgcccgttgt cacggtcagc tcgcccgaca
      421 caccgatggt ggaggtcgta ttgcgcgtca tattgacgta attcacgcga taagtgcgca
      481 aatacggcgt gtccggcgaa acgacgatgg tgttcccttc gatgcgatag cgcatgtcga
      541 cctggcgcga aatgcgttcc aggatcgccg gcaacgtttc gttgatggcg ttcaggctga
      601 cgagcccctg caggccggag tgaatgtcga tgttctgctt ggtgtcacgc gccagcgcga
      661 gcagcagctc cttcactgga acttcgttga cgaccacgct gtaggtttgt ggtttcgcag
      721 ccggcttggg cggcgggacg aagatcgtgg cgcgggccgg gggcggaatt gcggcatccg
      781 gttcaggctt tacgcgcgac gcggagatgt ggccttcgga taggggagct tgcggctgga
      841 tgtggccgca cgacgcagcg aggaacggaa gccccaatcc ggcgacgatt ccgatattcc
      901 taaaggcggc gccgacactg cgcgtttgct ggactgaggc gccggcccgg aggccagtgg
      961 caacaggccc tgctttctgc atcatcgtgc ccccccgctc aaccgccacc acgcgattca
     1021 gcaaaaagtg tctgcgtgcg cctcaagatg cccagtggcg catgatctct gtccgtatgc
     1081 cgcgcacggt gcgcaaaatg ggccggttga ccttgagtgc ggcaggcagg gtctccagcg
     1141 cattctgcgc cgcgcggcga tcgctgaaac tgccgtacag gacggtcatc gacgccttgc
     1201 ggttcgcaac cgtacgatac aaaaaaacct tattgacctc aatgaaattt gccatgtcat
     1261 tgagatgaag cgtgagctgg ctttcgtcct ggattcccat gagttgaatg ctgtatgtca
     1321 tcggtggagc ttcggcgagc cattcccgcg tcatggcgat gcgctgctcg acgagggtcg
     1381 ggagcgcacc ggacgcgtcg ggcccggcgc tgggcgcagg cgttgctatc ggcgcccgcc
     1441 cttcgctggc gctggacggc gaatccatgc cggctggcga ctcaccgttc gcaggcgtcc
     1501 ctgcgctgga cggaaggtgc actgcacggt ccgattttct cgcgtcgacc ggtagtgcgc
     1561 tttcgccgag ccatgcgagc gccaatgcgc cggtcagtgc gccgataagc aggagcgcgg
     1621 ccccccagac gatccggagg ccgcgtgccg gatgcgcgtc ctggccgaag tcgctgtcgc
     1681 gaacggcggc cgtgacgtgg cgcagtccga ccgtgtgggt gttctcggag aaggctgcga
     1741 gcagcgcctt gtcggcgatc aggttgatgc gtcgcgtaag accgctcgac gcgcgggcga
     1801 tgtagcgcgc gatccggttt ggaaagagat caggcccacg gtatcctgcc gcgcgcagtc
     1861 gaaacatgag gtaatcccga gtctcggcgg tattgagtgg cccgaggcgg aagctgtgag
     1921 tgatgcgctc cttcaactgt cggatcctgc catcgcgcag gttttcatcg agctcgggct
     1981 gcccgaagag cacgatctga agcagctttt cgttgcgcgt ctcgaggttg gagaggagac
     2041 ggatttcctc cagtgtggcg agtggcatgc tctgcgactc ctc
//
LOCUS       DASURG010000089         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_632058, whole genome shotgun sequence.
ACCESSION   DASURG010000089 DASURG010000000
VERSION     DASURG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632058"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFY80_01485"
     CDS             complement(<1..188)
                     /locus_tag="VFY80_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6006602.1"
                     /translation="MTLRIGILEGDDIGLEVVPECVKVMKAAAAKTGLDVDWRPIAIA
                     RKAHDEYGHTIPAGTLEAL"
     gene            complement(217..1272)
                     /locus_tag="VFY80_01490"
     CDS             complement(217..1272)
                     /locus_tag="VFY80_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6006603.1"
                     /translation="MLKIGVLMGDDIGPEVVPEAVKVMKRAAEKAGVSVQWSEHPIGK
                     RGHELHGHTFPQATIDALEKLDGWILGPIGHGAYPRNDPTWIMPPVRKRFELFASVKP
                     VKSYPNVRSLHKDVDIVFLREVTEGMQSSDTVVMGAGEFRPNDEITIAARVITRRGSN
                     RVARAAFEIARTRKRRKVTAVHKEPVYRLACGMFAEECRKVAKEYPDVAYDEALVDGF
                     SMKLMMNPWQYDVVVTTNQFGDILTDQGAGLVGGLGLAPGLCIGERQAMAQATHGSAP
                     DIAGRNIANPYAMIMSGQMLLEWLGRNRGDEKAVAAAQRIDAAVEHVIAEGKQLTADL
                     GGKASTSAMGDAIAAAV"
     gene            1412..>2390
                     /locus_tag="VFY80_01495"
     CDS             1412..>2390
                     /locus_tag="VFY80_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006604.1"
                     /translation="MSIRSSHKLHVLALGALLLCPAAALAQAYPSKPIRIIVPFPAGG
                     GVDYSGRLVGKGMSERLGQQVVIDNRPGANAILGLEVLKASAPDGYTLAAASAGPLAV
                     NPFIYSKLPHDTLKDFTQIASMCNFPLLLVVHPSLPVKSVKELIALARSRPGEVSYSS
                     PGAGNSSHLAGELFNSMAKVKILHIPYKGMAPAVVSTVSGESQLIYASIPPILPHVRS
                     GRVRALGVGNAQRVPTLPDIPTISEAGLPGYEAYAWGGMIGPANMPRDIVMKLNKAIV
                     DTLKQKDVIDKMLADGTVPTPSSPEEFTAYMKAELKKWGAVVKMANIKLE"
BASE COUNT          400 a          813 c          717 g          460 t
ORIGIN      
        1 agcgcttcga gcgttccggc gggtatcgtg tgtccgtatt cgtcgtgcgc cttgcgcgcg
       61 atcgcaatgg gccgccagtc cacatcgagc ccggtctttg cagcggccgc cttcatcacc
      121 ttgacgcatt cgggcacgac ttcgagcccg atgtcatcgc cttcgagtat gccgattctg
      181 agtgtcatct ctcgctcgcg tatcgaatcg gcacgttcaa acggccgcgg cgatagcatc
      241 gcccatcgcg ctcgtgctcg ccttgccgcc caggtccgcc gtcaattgct ttccttctgc
      301 gatgacatgt tccacggcgg catcgatgcg ctgcgcggct gcgaccgctt tttcgtcgcc
      361 gcggttgcgg cccagccatt cgagcagcat ctggcccgac atgatcatcg cgtagggatt
      421 cgcgatattg cggcccgcga tgtccggcgc ggagccgtgc gtcgcctgcg ccatcgcctg
      481 gcgctcgccg atgcatagcc cgggagcgag cccgagcccc ccgacgagtc ccgcgccctg
      541 gtcggtgagg atgtcgccga actgattggt agtgaccaca acgtcgtact gccacggatt
      601 catcatgagc ttcatcgaga agccgtcgac cagcgcttcg tcgtaggcca cgtccgggta
      661 ctccttcgcg accttgcggc attcctccgc gaacatgccg cacgccaggc ggtagaccgg
      721 ctccttgtgg accgcggtca ctttcctgcg cttgcgcgtg cgcgcgatct cgaacgccgc
      781 gcgcgccacg cggttcgagc cgcggcgcgt gatgacgcgc gcggcgatcg tgatttcgtc
      841 gttgggccgg aactcgccgg cgcccatcac cacggtgtcg gaggactgca tgccctcggt
      901 cacttcgcgc aggaacacga tatcgacgtc cttgtgcagc gatctcacgt tgggatagga
      961 cttgaccggc ttcacgctcg cgaaaagctc gaagcgcttg cgcacggggg gcatgatcca
     1021 cgtcgggtcg ttgcgcggat aggcgccgtg cccgatcggg cccagaatcc atccgtcgag
     1081 tttttcgagc gcgtcgatgg tcgcttgcgg aaacgtgtgg ccgtgcaatt cgtggccgcg
     1141 ttttccgatc ggatgctcgc tccactggac gcttacaccg gctttctcgg ccgcccgctt
     1201 catcaccttg accgcttcgg gcaccacttc cggcccgatg tcgtcgccca tcaatacgcc
     1261 gattttcagc acgattcttc tccttcacgc gagcccgcaa gtatatcgtc cgctgcgccg
     1321 gcatgccgcc catgaggctg acgaagttga caagcgcggc ggattcagcg acctttcttt
     1381 tcctatatac acaatacaaa aggaggagcc catgtcgatc cgtagctcac acaagttgca
     1441 tgtcctcgcg cttggcgcgc tcctgctttg cccggccgcc gcgctcgccc aagcctatcc
     1501 cagcaagccg atccgcatca tcgttccatt cccggcgggg ggcggcgtgg actacagcgg
     1561 ccggctcgtc ggcaagggca tgtccgagcg gctcggccag caggtcgtga tcgacaaccg
     1621 tcccggagcg aacgccattc tcggcctcga ggtcctgaaa gcctcggcgc cggacggcta
     1681 cacgttggca gcagcctccg ccggcccgct cgcggtgaat cccttcatct attccaagct
     1741 gccccacgac acgctgaagg acttcacgca gattgccagc atgtgcaact tccccctcct
     1801 gctggtggtg cacccttcgc tgcccgtcaa gagcgtcaag gagctcatcg cgctcgcccg
     1861 gtcgcgtccc ggtgaagtga gctactcgtc ccccggggcg ggcaactcct cgcatctcgc
     1921 cggagaactg ttcaactcga tggcaaaagt gaagatcctg cacatcccgt acaagggcat
     1981 ggcgcccgcg gtcgtatcga ccgtatcggg ggaatcgcag ctcatctatg cgagcattcc
     2041 gcccatactt ccgcacgtgc gctccggccg ggtgcgcgcc ctaggcgtgg gcaacgccca
     2101 gcgcgtgccg acgctgcccg acataccgac gatatccgag gcgggcttgc ccggatacga
     2161 ggcctatgcg tggggcggca tgatcgggcc ggccaatatg ccgcgagaca tcgtcatgaa
     2221 gctcaacaag gcgatcgtcg ataccctcaa gcagaaggac gtcatcgaca agatgctggc
     2281 ggacggaacg gtgcccacgc cctcgagccc cgaggaattc accgcttaca tgaaagcgga
     2341 gctcaagaag tggggcgcgg tcgtgaagat ggccaacatc aagctggaat
//
LOCUS       DASURG010000090         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1142497, whole genome shotgun sequence.
ACCESSION   DASURG010000090 DASURG010000000
VERSION     DASURG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142497"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..573)
                     /locus_tag="VFY80_01500"
     CDS             complement(76..573)
                     /locus_tag="VFY80_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX6006605.1"
                     /translation="MPSPLGHALGGLLAGLGTLPRRQPDTWLRDLGLCALVACLPDID
                     FAWGRHNMETHSLGAALAAGLIVFLVSRRRDLAIACTLAWSTHVLFDWLGSDDYPPLG
                     VMALWPLTDAFFFADAFLFDAITRQFHEPGVWQQNLRAVLKELVILAPPALVLWWWRV
                     RTRRA"
     gene            793..1821
                     /gene="recA"
                     /locus_tag="VFY80_01505"
     CDS             793..1821
                     /gene="recA"
                     /locus_tag="VFY80_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX6006606.1"
                     /translation="MAVDERQERMKALEVAVGQIEKQFGKGSIMRLGARNTIAPTEAI
                     STGSISIDYALGIGGLPRGRVTEIYGPESSGKTTLALQVIAEAQRTGGLAAFVDAEHA
                     LDAEYAKKLGVDIDNLLVSQPDNGEQALEIVEVLVRSGSVDVVVVDSVAALVPRAEIE
                     GEMGEAQMGLQARLMSQALRKLTGSISKSKTVLIFINQLREKIGVMFGNPETTTGGRA
                     LKFYASIRIDIRRIGAIKDGDVATGGRTRVKVVKNKMAPPFREAEFDIMYGEGISREG
                     DLLDLAVERKIVEKSGAWFAFGGERLGQGRENVKQFLKEHTDIRAAIEDRVRRDLGLV
                     REAEPQPV"
     gene            1987..>2170
                     /locus_tag="VFY80_01510"
     CDS             1987..>2170
                     /locus_tag="VFY80_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006607.1"
                     /translation="MPPALHSFDLWLFHALTPWRADWLDALMSVISIVGTGSAIWIVI
                     ALYALTRPRHRAAGWRL"
BASE COUNT          417 a          685 c          716 g          352 t
ORIGIN      
        1 cagctccctt gccgcggaca ggaggcgccc caatcagcgc actggcggtg agtctgtgcg
       61 accgtgtgcc ggctgtcaag cgcggcgcgt gcggacccgc caccaccaca ggacgagcgc
      121 cggcggcgca aggatcacga gctccttgag cacggcccgg aggttctgtt gccaaacgcc
      181 gggctcgtgg aactgccgcg tgatggcatc gaagaggaac gcgtcggcaa agaaaaacgc
      241 gtcggtcaac ggccacaacg ccatcacacc aagaggtgga taatcgtccg acccgagcca
      301 gtcgaacagc acgtgcgtgg accaggcaag cgtgcaggcg atcgcaaggt cgcggcggcg
      361 tgaaacgagg aagacgatga ggccggcggc gagcgcggcg ccaaggctat gcgtttccat
      421 gttgtggcgg ccccacgcga agtcgatgtc gggcagacag gcgacaagcg cgcagaggcc
      481 gaggtcgcgc agccaggtgt ccggctgccg ccgcggaagg gtccccaacc cggcgagcag
      541 ccccccaagc gcgtgtccca acggactcgg catctgctca ccgtacggaa gttctgcaat
      601 tccggtcaac cgacgggctg cacgaactgc catcaccagg caaaaaggcg aaagtggagt
      661 ctcgctgtaa gttattgaaa aaccgctttc tacagccttc gcgtcgctat tttccccttg
      721 actttcgctc ttgtttccac catcatgccg gtcggggctt cactcccctt caccttacga
      781 aaaggacggg ttatggcagt ggacgaacgc caggagcgca tgaaggctct cgaagttgcg
      841 gtgggccaga tcgaaaagca gtttggcaag ggctcgatca tgcggctggg cgcccgcaat
      901 acgattgcgc cgaccgaggc catttccacc ggctccatca gcatcgacta tgcgctcggc
      961 attggcgggc tgccgcgcgg ccgggtcacg gaaatctacg gtccggaatc gtcgggcaaa
     1021 accaccctgg cactgcaggt cattgccgag gcgcagcgta cgggcgggct ggcggcgttc
     1081 gtggacgccg agcacgcgct cgatgcggag tatgcgaaga agctgggcgt cgatatcgac
     1141 aacctgctcg tgtcgcagcc cgacaacggc gagcaggcgc ttgaaatcgt cgaggtcctg
     1201 gtgcgctccg gcagcgtcga cgtcgtcgtc gtcgactcgg tcgcggcgct cgtgccgcgc
     1261 gccgaaatcg aaggcgagat gggggaagcg cagatgggtc ttcaggcgcg gctcatgtcg
     1321 caggcgctgc gcaagctcac cggcagcatc tcgaagtcca agaccgtcct catcttcatc
     1381 aaccagctgc gggaaaagat cggcgtgatg ttcgggaatc ccgaaaccac gacagggggc
     1441 cgcgcgctca agttctatgc gtcgatccgc atcgacatcc gccgcattgg cgccatcaag
     1501 gacggcgacg tggccaccgg cggccgcacg cgggtgaagg tggtgaagaa caagatggcg
     1561 ccgcccttcc gcgaggccga gttcgacatc atgtacggcg aaggcatctc gcgcgaaggc
     1621 gacctgctgg atcttgccgt cgagcggaag attgtcgaga agagcggcgc gtggttcgcg
     1681 tttggcggcg agcgcctcgg ccagggccgc gagaacgtga agcagttcct caaggagcac
     1741 acggatattc gcgctgctat cgaggatcgc gtgcgcagag atcttggcct ggtacgcgag
     1801 gccgaaccgc agccggtgta gcgcgttggg cgcgcgacgt acgtcacgcg gagtacgtag
     1861 cgcggagctt tagctccgcg accgataggt cagttccgcg cgcacgccac cacgacgaat
     1921 ctaccctcta ttccgacgat cgttcgcgga gctgaagctc cgcgctacgt acgtaaacag
     1981 gggcacatgc cacccgctct gcactcgttc gatctgtggc tcttccacgc gctcactccc
     2041 tggcgcgcgg actggctcga tgcgctgatg agcgtcatca gcatcgtggg caccggatcg
     2101 gcaatctgga tcgtgatcgc cctctacgcg ctcacgcgcc cgcgccatcg agctgccggt
     2161 tggcgcctcc
//
LOCUS       DASURG010000091         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_121030, whole genome shotgun sequence.
ACCESSION   DASURG010000091 DASURG010000000
VERSION     DASURG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121030"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFY80_01515"
     CDS             <1..625
                     /locus_tag="VFY80_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6006608.1"
                     /translation="GAGIHAPEHHSESPEAMFAHIPGIRVVYASSPRRAYGLLLAAIR
                     DPDPVIFLEPTRLYRLFKEEVEDDGRALPLDTCFTLHEGTDITLVAWGAMTYEALAAA
                     NDLADEGVSAEVLDVATLKPLDMDTILASVAKTGRCVIVSEAARTCSFASEIAANLAE
                     EGLLSLLAPVQRVTGYDVVVPLPRLEKHYMPDKTRIVAAVRKAVAFG"
     gene            632..2005
                     /gene="nhaA"
                     /locus_tag="VFY80_01520"
     CDS             632..2005
                     /gene="nhaA"
                     /locus_tag="VFY80_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX6006609.1"
                     /translation="MQQPSYPLELLFGRVLSPFERFLRRTTAGGIILVATTAIALALA
                     MLVGGDTVARIWETPLAVAAGERYRLGMSLQHWVNDGLMAFFFLLVGLELKREVLVGE
                     LSSLKDAALPIAAALGGMVVPGLIYAAFNANTPTASGWGIPMATDIAFAIGILVLLAW
                     RVPKNLIIFLTALAIADDLGAVLVIALFYTAQLDLKSLGAAGLLFATLVLFNRGGIRH
                     PLPYALVGVLLWLAVHTSGVHATLAGILLALTIPARPTFTPAHFEQRIDELHAQFRAD
                     RRDLGTPDDPLSNERMASIAETMEQSAAAVQSPLQRIEHGLTPWVTFVVIPVFALANA
                     GIDLRAIAWSEALTNSVTLGVLAGLVIGKFSGIGLFSWVAVKLGLARLPSGVEWKHLL
                     GAAWLGGIGFTMSLFIAQLAFRDAALVDDAKLGILLASVTAALIGLTWLVWAGSARPR
                     APGSGRA"
BASE COUNT          360 a          719 c          694 g          430 t
ORIGIN      
        1 cggcgccggc attcatgcgc ccgagcatca ttcggagagc ccggaagcga tgttcgcgca
       61 tatccccggc atccgcgtcg tctacgcaag ttcgcccagg cgggcgtacg gtctgctgct
      121 cgccgcgata cgcgatccgg atccggtgat cttcctcgag ccgacacggc tctaccgcct
      181 gttcaaggaa gaggtggagg acgatggccg cgcgcttccg ctcgatacct gcttcacatt
      241 gcatgaaggg acggatatca cgctcgttgc ctggggcgcg atgacgtacg aagcgttggc
      301 cgcggcgaac gatctcgccg acgaaggcgt gagcgcggaa gtcctcgacg ttgcgacact
      361 caagccgctc gacatggaca ccatcctcgc gtcggtcgcg aaaaccggac ggtgcgtgat
      421 cgtctcagaa gcggcacgga catgcagctt tgcatctgaa atagcagcca acctggctga
      481 agaggggctt ctctcgttgc tcgcaccggt gcagcgcgtc accggttatg atgtggtggt
      541 tcccctcccc cgcctcgaga aacattacat gcccgataaa acgcgcatcg tcgccgccgt
      601 aagaaaagcg gtcgcattcg gctagttcgg catgcagcag ccgagctatc cgctcgaatt
      661 gctgttcggg cgcgtcctgt cgccgttcga gcggtttctg cgacgcacga ccgcgggcgg
      721 catcatcctc gttgcgacca cggcaatcgc gctcgcgctt gcgatgctgg tgggcggcga
      781 tacggtggcg agaatctggg agacaccgct tgcggttgcg gcaggcgaac gctaccgcct
      841 cgggatgtcg ctccagcact gggtaaacga cggtctgatg gcgttcttct tcctgctggt
      901 cgggctcgag ctcaaacgcg aagtcctggt cggcgaattg tcttcgctca aggatgcagc
      961 actgcccatt gccgctgcgc ttggcggaat ggtcgtgccg ggcttgatct acgccgcatt
     1021 caacgccaat acacccaccg catccgggtg gggaatcccg atggcgaccg atatcgcctt
     1081 cgcgatcggc atcctggtgc tgcttgcctg gcgggtgccc aagaacctca tcattttcct
     1141 cacggcactc gccatcgcgg acgacctggg tgccgtgctc gtcatcgccc tcttctacac
     1201 ggcgcagctc gatttgaaat cgctcggtgc cgcggggctg ctcttcgcga cgcttgtgct
     1261 cttcaatcgc ggcggcatcc gccatccgtt gccctatgcg ctggtcggcg tgctgctgtg
     1321 gcttgctgtg cacacttcag gcgtacacgc gacacttgcc ggcatcctgc tcgcactcac
     1381 gatacccgcg cgtcccacat tcacccccgc gcatttcgag cagcgcatcg atgaactgca
     1441 tgcgcaattt cgcgccgatc gccgcgacct gggcacgccc gacgatccgc tcagtaacga
     1501 gcgcatggca tccattgcgg aaacgatgga acagtcggcg gccgcggttc agagccccct
     1561 gcagcgcatc gagcacgggc tcacgccctg ggtgacgttc gtcgtcattc cggtcttcgc
     1621 gctcgccaac gcggggatcg acctgcgcgc cattgcgtgg tccgaagcgc tcacgaacag
     1681 cgtgacgctc ggcgtgctcg caggcctggt gatcggaaag ttcagcggca tcggtctctt
     1741 cagctgggtg gcggtgaaac tcggcctcgc gcgcctgccc tcgggcgtgg agtggaaaca
     1801 cctgttgggt gcagcatggc tgggcggtat cggctttacg atgtcgctct tcatcgcgca
     1861 gctcgcgttt cgcgacgccg cactcgtgga cgacgcgaag ctcggcatcc tgctcgcctc
     1921 cgtaaccgcc gcattgatcg ggctcacatg gctcgtctgg gcggggtccg cccgaccgcg
     1981 agcgccgggc agtggtcgag cgtagcgact ggcatgcgct caccggcgaa caggcactcg
     2041 cggcagtgca ttgagtgccg gcctcaccgc accaccactc cggccagatc gatccactgc
     2101 tactgtagag ctatgcgcgg gcttgttttg tcatgcgcgg cgcgggagct atcgctgcta
     2161 tattcgcggc gaagtgcgaa tgcctgcggg acggagaagg agc
//
LOCUS       DASURG010000092         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_325952, whole genome shotgun sequence.
ACCESSION   DASURG010000092 DASURG010000000
VERSION     DASURG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325952"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /gene="rplU"
                     /locus_tag="VFY80_01525"
     CDS             <1..263
                     /gene="rplU"
                     /locus_tag="VFY80_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6006610.1"
                     /translation="EKIKVEQIPAEVGAEIALEEVLVVADGAEVKLGNPLVTGASVKA
                     KVIGHGRGEKVHIFKMRRRKHYRKSQGHRQNYTEIEILGIGS"
     gene            294..551
                     /gene="rpmA"
                     /locus_tag="VFY80_01530"
     CDS             294..551
                     /gene="rpmA"
                     /locus_tag="VFY80_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6006611.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKCFGGEVVPAGSIILRQRGTQ
                     VHPGRNVGVGRDHTLFAKITGKVEFSQKGPRNHKTVNVLPA"
     gene            614..1771
                     /gene="obgE"
                     /locus_tag="VFY80_01535"
     CDS             614..1771
                     /gene="obgE"
                     /locus_tag="VFY80_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6006612.1"
                     /translation="MKFIDEAIIEVHAGKGGDGVASFRREKYIPRGGPDGGDGGRGGS
                     IYAVADRNVNTLIDYRYARIHRARNGEKGQGSDCYGRSAPDIELRMPVGTVVRDLETG
                     QLVADLDHDGERALLAKGGKGGLGNLHFKSSINRAPRQFTRGEPGESRRLKLELKVLA
                     DVGLLGMPNAGKSTFIRAVSAARPKVADYPFTTLHPNLGMVRVDVSRSFVVADIPGLI
                     EGAAEGAGLGHQFLRHLARTRLLLHIVDMAPLDENADPVREAKAIVKELRKYDEALYR
                     KPRWLVLNKADMVPEDEREKAAKRLVRGLGWKGKSFIISALTGEGCRELTYEIMACLK
                     DGAADRERKAADERATEQSTGHSSDSSLSSPRKPQLSSSRRKPGSRKTTPH"
     gene            1779..2897
                     /gene="proB"
                     /locus_tag="VFY80_01540"
     CDS             1779..2897
                     /gene="proB"
                     /locus_tag="VFY80_01540"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX6006613.1"
                     /translation="MTSVLTDAKRLVVKVGSSLVTNRGRGLDHARLAVWAAQVAELRH
                     MGREVILVSSGAIAEGLQRLGWKRRPRALHELQAAAAVGQMGLIEVYESCFREHGLLT
                     SQILLTHDDLADRKRYLNARSTLRTLLGLGVIPIINENDTVAMDEIRFGDNDTLAALV
                     TNLIEADALIILTDQGGLYRSDPRKDPRAKLVEEADADDPKLEAMAGGAGSAIALGGM
                     LTKVLAARRAARSGAHTVIASGHERDVLLRLARGERIGTQLVARSATLAARKQWLADH
                     LQVRGTLLLDSGAVKALKVDGKSLLAIGVHEAAGDFERGEVVSCLDESRREIARGLIN
                     YNASETRRILRTPSHEIENKLGYIDEPELIHRDNLVLL"
BASE COUNT          631 a          912 c         1020 g          472 t
ORIGIN      
        1 gcgagaaaat caaggtcgaa cagatcccgg cggaggtcgg ggctgaaatc gctctggagg
       61 aggtactggt cgtagcggac ggcgcggaag tgaagctcgg taatccgctc gtcaccggcg
      121 catcggtcaa ggccaaggtg atcggccatg gccgcggcga gaaggtccat atcttcaaga
      181 tgcggcggcg caagcattat cggaaaagcc agggacatcg gcagaattac accgagatcg
      241 agattctcgg cattggaagt tgacggttat tcgccccgga taaggagtgg cagatggcac
      301 acaagaaggc aggcggcagt tcacgcaacg gccgcgattc gcagtcgaag cgtctgggcg
      361 tcaagtgttt cggcggtgaa gtcgttcccg ccggcagcat catcctgcgc cagcgcggga
      421 cgcaggtgca tcctggacgc aatgtcggcg tcggccgcga tcacacgctg ttcgcaaaga
      481 tcacgggcaa ggtcgagttc tcccagaagg gcccgcgcaa ccacaagacg gtgaacgtcc
      541 ttcccgcctg acgcgcgcgc agagcgaacg aagaggccct gtcggacgac agggcctttt
      601 ttgtttgagt gccatgaaat tcatcgacga agcgatcatt gaagtccacg ccggcaaggg
      661 tggggacggg gtcgcgagct tccggcgcga aaagtacatt ccgcgcggcg ggccggacgg
      721 tggcgacggc gggcgcggcg gaagcattta tgctgtcgcc gatcgcaacg tcaatacgct
      781 tatcgactat cgctacgctc gcatccaccg ggccaggaac ggcgagaagg gtcagggctc
      841 ggactgctat ggccgctcgg cgccggacat cgaactgcgc atgcccgtcg ggacggtggt
      901 gagagacctg gaaaccggtc agctcgtggc cgatctcgat cacgatgggg agcgtgcgtt
      961 gctcgcgaaa ggcggaaaag gcggtctcgg caatctgcac ttcaagtcga gcatcaatcg
     1021 cgcgccgcgc cagttcacgc gcggcgagcc gggagaaagc cgcaggctca agctggaact
     1081 aaaggtgctc gccgacgtgg gtcttctcgg gatgccgaac gccggcaaat cgacgttcat
     1141 ccgcgctgtg tcggcggcac gcccgaaggt cgccgactat ccgttcacga cgctgcatcc
     1201 caatctcggc atggtgcgcg tcgatgtgag tcgcagcttc gtcgtcgccg acattcccgg
     1261 tctcatcgaa ggcgcggcgg aaggcgcagg gctcggacac cagtttctga ggcacctcgc
     1321 gcgcacgcgg ctcctgcttc acatcgtcga catggcgcca ctcgacgaga acgcagaccc
     1381 ggtgcgcgag gcgaaggcga tcgtgaagga gctcagaaaa tacgacgaag cgctctatcg
     1441 gaagccgcgc tggctcgtgc tcaacaaggc agacatggtg ccggaggacg agcgtgagaa
     1501 agcggcgaag cggctcgtgc gaggtttggg ttggaaggga aaaagcttta tcatctcagc
     1561 gcttacggga gagggatgca gggagctcac gtacgagatc atggcctgcc taaaagatgg
     1621 cgcagccgat cgcgagcgga aagcagcgga cgaaagagct actgaacaaa gcaccggcca
     1681 ctcatcggac tccagcctgt cctccccgcg aaagccgcaa ctatcgtcat cccggcgaaa
     1741 gccgggatcc agaaaaacaa cgcctcactg acatccccat gacttcagtc ctgacagacg
     1801 ccaagcgcct cgtcgtcaaa gtgggcagca gcctcgtgac gaatcgcggg cgcgggctcg
     1861 atcacgcgcg gcttgcggtg tgggcggcgc aggtcgcaga actgaggcac atggggcgcg
     1921 aagtcatcct cgtctcgagc ggcgccatcg ccgaaggatt gcagcgcctc gggtggaagc
     1981 gcagaccgcg cgcgctccac gagctgcagg cggccgccgc agtggggcag atgggactca
     2041 tcgaagtcta cgagtcgtgc ttccgcgagc atggtctgct cacctcgcag atcctgctca
     2101 cgcacgacga tctggccgat cgcaagcgct acctcaacgc gcgctcgacg ctgcgaacgc
     2161 tgctcggcct gggcgtcatc ccgatcatca acgaaaacga cacggtggcg atggacgaga
     2221 tccggttcgg cgacaacgat acgctcgccg cgctggtcac caatctgatc gaggccgatg
     2281 cgctcatcat cctgaccgac cagggcggcc tctaccgcag cgacccacgc aaggatccgc
     2341 gcgcgaaact ggtcgaagag gcagatgcgg acgacccgaa gcttgaagcc atggcgggcg
     2401 gcgcgggcag tgccatcgcg ctcggcggga tgctgaccaa agtgctggcc gcccgccgtg
     2461 cggcgcgtag cggcgcgcac acggtgattg catcggggca tgagagagac gtgctgctgc
     2521 gcctcgcgcg cggggagcgc atcggcacgc agctcgtcgc ccggagcgca acactggcgg
     2581 cgcgcaagca gtggctcgcc gatcacctgc aggtgcgggg cacgctactc ctcgacagcg
     2641 gtgcagtgaa agcgctcaag gtggacggca agagcctgct ggcgatcggc gtccacgaag
     2701 cggcgggcga ttttgagcgc ggcgaagtgg tgagctgcct cgacgagtcg cggcgggaaa
     2761 tcgcgcgcgg gctcatcaat tacaatgcga gcgagacgcg ccgtatcctg cgcacgccgt
     2821 cgcacgagat cgagaacaag ctcggttaca tcgacgagcc ggagctgatc caccgcgaca
     2881 atctggtctt gctttaggca ataccagcga agcgtgtcat cccggcgacg gtaagcatgc
     2941 ccggaaggat tgaatccggg gccagatcca gtctggattc cggatcattg tccggaatga
     3001 cgagttgcca ttcgtcatga gagcatccgt cttcc
//
LOCUS       DASURG010000093         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_19661, whole genome shotgun sequence.
ACCESSION   DASURG010000093 DASURG010000000
VERSION     DASURG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19661"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFY80_01545"
     CDS             complement(<1..551)
                     /locus_tag="VFY80_01545"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6006614.1"
                     /translation="MQTRVDEIAPDVYRISTYIPDIDLQFNQFLVKDEQPLLWETGMK
                     ALFPAVREAVSKVVAPETIRWVAYSHYEPDECGSLNEWLGLAPRAQAFCTEIGAIVFA
                     NDAALRPVRGIGKNERIETGKYRFRLHPTPHLPHGWDAGTLFEETNRTLLCSDLFTHN
                     GDVEPSMQSDIVGRFSASLKTYQA"
     gene            663..1637
                     /locus_tag="VFY80_01550"
     CDS             663..1637
                     /locus_tag="VFY80_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006615.1"
                     /translation="MKRRAVAIFIMLCCGCSAALAQSGDTARYPTRPVRIVVPFGAGG
                     PSDLLARTVAQKMSESLGQQVIVENRGGANGVVGVEVVARSAPDGYTLVLGTTGTHGI
                     NASLVPKLPYDTIRDFAPIARLGISNYVLVAHPSLPVRTVKELLQLAKARPGELTWAA
                     GGGVTQLAAELFKHSAGINVIVVPYKGNAPAVTATMSGEVSLIFGGIAQAAKLVEAGR
                     LRAIAVSGAQRSPALPGVPTVAESGVPGFEAGSWYGLLAPAATPRGIVERLNGEVLRV
                     LKLPDVRSRLLAEAFDIPLDTPESFAAHIRADVPKWAKVIRDAGIRAN"
     gene            1637..>2533
                     /locus_tag="VFY80_01555"
     CDS             1637..>2533
                     /locus_tag="VFY80_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6006616.1"
                     /translation="MAHKQIGVAIIGAGRIGTLRAHMAATHPGVRFIAVADAELQRAR
                     TLAQRVNAHLVCHDNREAISHADVDAVIVSTSEHEHTAPVLQALERGKAVLVEKPLAL
                     ELEDAGRILAALEATRGNLRVGYSRRFKRRYLTAKEQVKRGRLGTIVGVSARLYNTRA
                     QVYQMLDRNPGATPVVDSLTYYVDLLGWFLPDNPVVEVSAWGQAGAIRARGYDCDDVT
                     WAILRLADGALVNLGVSFALPDKYPSLGYSGRFELLGTEGSLIIDDDHLDQLLYTERG
                     IPHIYIPDHTVNMAFLGSSAPGD"
BASE COUNT          443 a          789 c          850 g          451 t
ORIGIN      
        1 gcctggtagg ttttgagcga agcgctgaaa cgccctacga tgtcgctctg catgctcggt
       61 tccacgtctc cgttatgggt gaagagatcg gaacaaagca acgttcgatt ggtttcctcg
      121 aacagcgtgc ctgcatccca gccatggggc agatgaggcg tgggatggag ccggaatcgg
      181 tattttcccg tttcgatgcg ctcgttcttg ccgatgccac gcactggacg aagcgccgca
      241 tcgttggcga agacgatcgc gccgatctcg gtgcagaacg cctgcgcccg cggggcaagc
      301 ccgagccatt cattgagcga tccgcattcg tccggctcgt aatggctgta cgcgacccaa
      361 cgtatggttt cgggggccac tacctttgaa accgcctcgc gcaccgcggg aaaaagcgct
      421 ttcattccgg tctcccacaa gagcggctgc tcgtccttca ccaggaactg gttgaactgc
      481 agatctatgt ccgggatata ggtggagatt cgataaacat ccggtgcgat ctcatcaacg
      541 cgggtctgca tcgtggtcct ttccagttga cacctgaaga ggccatcaag tctaatcggg
      601 ctccaccgga gtaaacaaga agacgcggcg tgccagcgcg agcgcgtagt tgaggagccc
      661 cgatgaagcg tcgagcagtt gccatcttca tcatgctgtg ttgcggttgc agcgcggcgc
      721 tcgcgcagag cggcgatacc gcgcgctatc ccacgcgtcc ggtgcgcatc gtcgttccgt
      781 tcggcgcggg cggcccgtcc gatcttcttg cgcgcaccgt ggcgcagaaa atgtcggaga
      841 gcctcgggca gcaggtgatc gtcgaaaatc gcggcggggc gaacggcgtg gtcggcgtgg
      901 aggtggtcgc gcgcagcgcg ccggacggct ataccctggt tctgggcacg accggaacgc
      961 atggcatcaa cgcgagcctc gtgccgaagc tgccttacga cacgatcagg gacttcgcgc
     1021 cgatcgcccg cctcggcatc tccaactacg tgctggtcgc ccacccctca ctgcccgtgc
     1081 gtaccgtgaa ggaactgctg cagctcgcga aggcgcggcc gggcgagctc acctgggccg
     1141 caggcggcgg cgtgacgcag ctcgccgcgg agcttttcaa gcacagcgcc ggcatcaacg
     1201 tgatcgtcgt cccctacaag gggaatgcgc cagcggtgac cgccaccatg agcggcgaag
     1261 tatccctgat ctttggcggc atcgcgcagg cggcaaagct ggtggaggcg ggccggttga
     1321 gagccattgc cgtgagcggt gcgcaaagat cgcccgcatt gccaggcgtc ccgacggtcg
     1381 ccgaatccgg cgtgccggga ttcgaggcgg gctcctggta tggattgctc gcaccggcgg
     1441 ccacgccgcg cggtatcgtc gagcgcctca acggcgaggt gctgcgcgtg ttgaagcttc
     1501 ccgacgtgcg cagtcgcctt ctcgcggaag ccttcgatat accgctcgac acgccggagt
     1561 ccttcgcggc tcacatccgg gccgacgtgc cgaagtgggc gaaagtgatc agggatgcag
     1621 gcatcagggc gaactgatgg cgcacaagca gatcggggtc gcgataatcg gggcggggcg
     1681 gataggcacg ctgcgcgcgc atatggccgc gacgcatccg ggcgtgcgct ttatcgccgt
     1741 tgccgacgca gagctgcagc gtgcgcgaac gctcgcgcag cgggtgaacg cgcatctcgt
     1801 ttgccacgac aacagggagg cgatatcgca tgccgacgtg gatgcggtga tcgtttccac
     1861 cagcgagcac gagcacaccg cgccggtgct gcaggcgctg gagcgcggca aggcggtgct
     1921 ggtggaaaag ccgctcgcgc tcgaactgga ggacgcgggt cgcatccttg ccgcgctgga
     1981 ggcgacgcgg ggcaatctgc gcgtcggcta cagccggcgc ttcaaacggc gctatctcac
     2041 cgcgaaagag caggtgaagc gcggccggct gggaacaatc gtcggcgtgt ccgcgcgcct
     2101 gtacaacacc cgcgcccaag tctatcagat gctggaccgc aatccgggcg cgacgccggt
     2161 ggtcgattcg ctcacctact acgtggatct gctcggctgg tttcttccgg acaatcccgt
     2221 ggtggaggtg tcggcgtggg ggcaggcggg ggcaattcgc gcccgcggct acgactgcga
     2281 cgatgtgacg tgggcgatcc tgcggctcgc cgatggtgcg ctcgtcaatc ttggcgtgag
     2341 cttcgcgctg ccggataagt atccgtcgct gggctacagt ggccgcttcg aactgctcgg
     2401 caccgaaggc tcgctcatca tcgacgacga tcatctcgac cagttgctct acaccgagcg
     2461 cggcattccg cacatctaca ttccggacca caccgtcaac atggcgtttc tcggcagctc
     2521 ggcgccgggg gat
//
LOCUS       DASURG010000094         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_734043, whole genome shotgun sequence.
ACCESSION   DASURG010000094 DASURG010000000
VERSION     DASURG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734043"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFY80_01560"
     CDS             complement(<1..542)
                     /locus_tag="VFY80_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6006617.1"
                     /translation="MNVRCPQCESTFRVDPERIPAAGIKARCSKCATVFELSRQGAPA
                     ARPKAAAPAPAMATGPAGPQPEPMSAAQPRLSVVPPTSAASEPALRQAETAPVAAPPA
                     PPSFPKPALAPVWPSLPGRPPSPPAPGVLKVGPPPPNPPSPPLMPSAPAAPTSTPSTE
                     PGATGTRATTFTPRPPCAVAP"
     gene            complement(539..1444)
                     /locus_tag="VFY80_01565"
     CDS             complement(539..1444)
                     /locus_tag="VFY80_01565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6006618.1"
                     /translation="MNEIPDRNEVYALVNRTFPAGIVCDTEPGNGTIELCRKLKSDPF
                     SAIVPIVLAVRPDQRALVIEGLEAGADDVIPDDMETRERTLRMHMILNRADRDVSVHP
                     TTRLPGTPQIERDINERIRRDEVFAVCYADLDHFKEFNDRYGYNEGDGVIKLLSRILR
                     DVVKGHCPDGFIGHIGGDDFIFNVPFDGMRIVCEEIIEIFDELIPYQYTEEDRRAGYF
                     LGRDRRGNIVWVPLMTLSIGVVTNQQRTFAHTARVSELATEMKSFAKKLPGSVYAVDR
                     RSDARGTYFGQTIVSSEELEAPKET"
     gene            complement(1599..3809)
                     /gene="rnr"
                     /locus_tag="VFY80_01570"
     CDS             complement(1599..3809)
                     /gene="rnr"
                     /locus_tag="VFY80_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX6006619.1"
                     /translation="MPVSPERIIDYLREETGRPLTPVELATELEIPEAQTDDFNKLLT
                     ELEEQGLLYRNRHQRYAVPTKFNLVVGTLQTIRSGAAFVVPDEGKEDLYIGREGLKSA
                     VHGDRVIARVEKRKRSGKTEGRVIRILERARSTVVGVYHPTKNYGFVVPEDQKLSNDV
                     FVPPDAEGEATEGDVVVVRIEDWGDQHRGPVGKVEKVLGKMGEPGVDVLAVLYGHELP
                     VDFPQEVVDAAEAMRQRGVTENDLAGREDLRDELVFTIDPVDAKDHDDALSIKQIGDD
                     EWEVGVHIADVSYYVTEGSIIDAEALRRGTSIYMVDRTIPMLPHPLSSDLCSLKEGVD
                     RLAMSVMVRIDGSGTINAHKLARTVIRSRHKLAYEDAQAVIEGTGSIDAETDKAIHDL
                     VALSRVLRELRAARGSLDFDLPEARVVLNTEGEPTDIQKVERLESHRLIEDFMLLANE
                     VIARKGSRNKLPFLYRVHESPDQERLGKLQLFAGTFGYTVTTAKKVTPKDLQRLLERV
                     RGKAEERLLSTVVLRSMRQARYSHENLGHFGLAAKFYTHFTSPIRRYPDLVVHRICAH
                     AFLNEENVRQAMNTTYLPEIARVSSTRERVAVDAERDSIEMKKVEFMERHVGDEFAGT
                     VSGVTAFGFFVLLDAYFVEGLVHISSLEDDYYQFLEEQYALIGERTRRRFRLGDRVKV
                     QVARVDLEERKIDFALLEDESGGKKKKGRKDRAGHTSKGGKGKGGRRKARASFD"
     gene            complement(3884..>4136)
                     /locus_tag="VFY80_01575"
     CDS             complement(3884..>4136)
                     /locus_tag="VFY80_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006620.1"
                     /translation="DIVQRGHSAGPPAFNVGLEMILEESVRFQIGRASTADGVGSGGL
                     ALGVGLHYDRFDIFVAKGFAGSALDPEGESVHITFGVRF"
BASE COUNT          735 a         1357 c         1227 g          817 t
ORIGIN      
        1 ggcgcgaccg cgcagggtgg acgcggcgtg aacgtggtgg cgcgggttcc ggtcgcgccc
       61 ggttcggtgc tcggcgtgct cgtcggtgcg gcaggcgcgg acggcatcaa cggaggcgac
      121 ggcggattcg gcggcggcgg accgaccttg agcacgcctg gtgcaggtgg cgacggcggc
      181 ctgcccggca acgacggcca gaccggggcg agcgccggct tcgggaacga cggcggcgcc
      241 ggcggcgccg cgaccggtgc cgtttccgct tgccgcagtg ccggttcaga cgcagccgac
      301 gtcggcggga ccacggaaag gcgaggctgc gccgccgaca tgggctcggg ctgcgggccg
      361 gccggaccgg tcgccatcgc gggggcgggc gccgcggctt tcggccgcgc cgcgggcgct
      421 ccctggcgtg aaagctcgaa taccgttgcg catttcgagc accgggcctt gatgccggcg
      481 gcgggaatgc gttccggatc tacacgaaag gtcgattcac actgtgggca gcggacgttc
      541 atgtttcttt cggggcttcc agctcttctg atgaaacaat tgtttgtccg aagtacgtgc
      601 cgcgcgcatc cgaacggcga tcgaccgcgt agaccgagcc cggaagcttt ttggcaaacg
      661 acttcatttc cgtcgcaagc tcgctaactc gggccgtatg cgcaaaggtg cgctgctggt
      721 tcgtgaccac accgatcgaa agcgtcatga gcggcaccca cacaatgttg ccacggcggt
      781 cccgccccag aaaatacccg gcccgccggt cttcctcggt gtactgatac gggatcagct
      841 cgtcgaatat ctcgatgatc tcttcgcaca caatgcgcat gccatcgaac ggcacattga
      901 agatgaaatc gtcgccgcca atgtgcccga tgaagccatc cgggcagtgg cccttgacga
      961 cgtcgcgcag aatgcgcgac agcagcttga tgacgccgtc gccttcgttg taaccgtagc
     1021 gatcgttgaa ttccttgaag tggtcgaggt ccgcgtaaca gactgcaaac acttcgtcac
     1081 gccggatgcg ctcgttgatg tctcgctcga tttgcggcgt gcccggtaag cgggtggttg
     1141 gatgcacgct cacgtcgcgg tccgcacgat tcaggatcat gtgcatccgc agcgtccgct
     1201 cgcgcgtttc catgtcgtcg gggatgacgt cgtccgcgcc cgcctcgagg ccttcgatta
     1261 ccagcgcacg ctgatccggg cgaacggcca ggacaatcgg cacgatcgcg ctgaagggat
     1321 cgctcttcag cttacggcat agctcgatcg tgccgttgcc cggctcggta tcgcaaacga
     1381 tgccggccgg aaaggtgcga ttgaccagcg cgtacacctc attccgatcc ggaatctcat
     1441 tcaaggtcag gcccgcctct tccacgaaat cgcggacgac gcgcggacac tctcgcccgg
     1501 tcggagcgtg atagactaga accggcttaa aacccatgcc ctgggaatct ggagttagct
     1561 gggagaatgc cgcaagctac tctaaaactt aggcttagtc aatcaaaact ggcgcgcgcc
     1621 tttctccgcc ccccttttcc cttgccgcct ttcgaggtat gccccgcgcg gtcttttcgc
     1681 cctttcttct ttttgccgcc actctcgtcc tcgagcagcg caaagtcgat cttgcgctcc
     1741 tccagatcca cccgcgccac ttgcaccttt acccggtcac ccaggcgaaa ccgccggcgt
     1801 gtgcgctcgc cgatcaacgc atactgctct tccaggaact ggtagtagtc gtcttccagc
     1861 gagctgatgt gaaccagccc ttccacgaag tacgcgtcca gcagaacgaa gaagccgaac
     1921 gccgtcacac cggagaccgt gcccgcgaac tcatcgccaa catggcgttc catgaattcg
     1981 actttcttca tctcgatgct gtcgcgctcg gcatctacgg ccacgcgttc gcgcgtgctc
     2041 gaaacgcggg caatctccgg caggtaggtc gtgttcatcg cctggcgtac attctcctcg
     2101 ttgaggaagg cgtgcgcgca gatgcgatgg acgaccaggt ctggatagcg ccgaatgggt
     2161 gacgtgaagt gggtgtagaa cttcgcggcc agcccgaaat gcccgagatt ctcgtgactg
     2221 tagcgcgcct ggcgcatgga ccgcagcacc acggttgaaa gcagccgctc ctccgccttg
     2281 cctcgcacgc gctccagcaa ccgttgcaga tctttcggcg tgactttctt ggccgtcgtc
     2341 acggtgtaac caaaggtgcc cgcaaacaac tgcagtttgc ccagccgttc ctggtccgga
     2401 ctttcatgca cgcgatagag gaaaggcagc ttgttgcgcg agcctttgcg ggcgatgacc
     2461 tcgttcgcca gcagcatgaa gtcttcaatc aaccgatgac tctcgaggcg ctccaccttt
     2521 tgaatatcgg taggctcgcc ttccgtgttc agcacgaccc gcgcctccgg cagatcgaag
     2581 tccaggctgc cccgcgcagc gcgcagctcg cgcagcactc tgctcagagc aaccagatca
     2641 tgaatcgcct tgtctgtttc ggcatcgatg ctcccggtcc cctcgatgac ggcctgggcg
     2701 tcttcgtagg ccagcttgtg ccggctgcga atcacggtgc gcgccagctt gtgcgcgttg
     2761 atggtgccac tgccgtcgat ccgtaccatg accgacatcg ccagccggtc caccccctcc
     2821 ttcaacgaac acaaatccga ggaaagcggg tgcggcagca tgggaatggt gcggtccacc
     2881 atgtagatgc tggtgccccg ccgtaacgct tccgcgtcga tgatgctgcc ttcggtgacg
     2941 taatagctca cgtctgcgat gtgcacgccg acttcccatt cgtcatcgcc gatctgcttg
     3001 atggaaagcg cgtcatcatg gtccttggca tccaccggat cgatggtaaa gaccagctca
     3061 tcacgcaggt cttcacggcc cgccagatcg ttctccgtga ccccgcgctg ccgcatggct
     3121 tctgccgcat ccaccacttc ctgcggaaaa tcgaccggca gctcgtgacc gtacaacacc
     3181 gccagcacat ccacacccgg ctcgcccatc ttgcccagga ctttttcgac tttgccgacc
     3241 ggtccgcgat gctggtcacc ccagtcctcg atgcgcacta cgaccacgtc gccttcggtc
     3301 gcctcccctt cggcgtcggg cggaacgaaa acatcgttgg atagtttttg atcctccggc
     3361 accacgaagc cgtagttctt cgtcggatgg taaacgccaa ccacggtaga gcgcgcacgt
     3421 tccaatatgc gaatgacgcg gccctcggtt ttcccgctgc gcttgcgctt ttcgacccgc
     3481 gcgatcacgc gatcgccgtg cacggcggat ttcaggccct cgcgcccgat ataaagatcc
     3541 tctttacctt catcgggcac gacgaacgcc gcaccactgc gaatggtctg gagcgttccc
     3601 acaacgaggt tgaacttggt tggaacagcg tatcgctggt ggcggttgcg gtagagcagt
     3661 ccttgctctt ccagctcggt cagcaacttg ttgaagtcgt cggtttgagc ttcgggaatc
     3721 tccagctcgg tcgccaattc gacaggcgta agaggccggc cagtctcttc gcgcagatag
     3781 tcaatgattc tttcggggga tacgggcacg tgtctgctcc agtcaggatc caggccgcgg
     3841 cttcagcgcc gggctggccc ttgggttggc aaggatcacg ccctcagaac cgcacgccga
     3901 atgtgatatg caccgattcg ccttccggat cgagcgcgga tccggcaaaa cccttcgcga
     3961 cgaagatgtc gaagcggtcg tagtgcaggc caacgcccag ggccagcccc ccgctgccca
     4021 cgccgtccgc ggtcgatgcg cgcccgattt gaaagcggac cgattcctcg agaatcatct
     4081 ccagtcccac gttgaatgcc ggcggccccg ccgaatgccc gcgctgcacg atgtcg
//
LOCUS       DASURG010000095         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1347239, whole genome shotgun sequence.
ACCESSION   DASURG010000095 DASURG010000000
VERSION     DASURG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347239"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1616)
                     /locus_tag="VFY80_01580"
     CDS             complement(30..1616)
                     /locus_tag="VFY80_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX6006621.1"
                     /translation="MPLIRSRIRPNAPEFETAARQMRAQVDDLRARLARAREGGGEAA
                     IARHCARGKLPARERIAALVDTGAPWLELSPLAAWDVYDEPVPAAGIITGVGEVSGRH
                     CVVIANDATVKGGTYYPLTVKKHLRAQQIALENRLPCIYLVDSGGAYLPQQDEVFPDR
                     EHFGRIFYNQAQLSARGIPQIAVVMGSCTAGGAYVPALSDETIIVRGQGTIFLAGPPL
                     VKAATGETVTAEALGGGDVHTRVSGVADHLADDDRHALEIARRALAHVNPAPAADMRR
                     ATPQPPAYDPEELYGIMPVNPRVPFDVREIIARVVDGSEFDEFKARYGTTLVCGFARI
                     DGYPVGILANNGILFSESALKATHFIQLCCQRGTPLVFLQNISGFMVGSKYEAGGIAK
                     DGAKLVTAVASAEVPKFTVIVGGSFGAGNYAMAGRAYQPRFLWTWPNARISVMGGQQA
                     ARVLSTVRGEFASDAERNAFEAPILDAYEREGNPYYATARLWDDGVIDPLDTRRILAL
                     GIEAALHAPIPETRFGVFRM"
     gene            complement(1620..2132)
                     /locus_tag="VFY80_01585"
     CDS             complement(1620..2132)
                     /locus_tag="VFY80_01585"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6006622.1"
                     /translation="MLDKLEARLANIVELNVDAIVNAANTSLLGGGGVDGAIHRAAGP
                     ELLQACRAIGGCPTGEARITRGYRLPAKYVIHAVGPVWHGGSKREPELLASCYRSSLA
                     LAVEHGVKTLAFPAISCGAYRYPIADAVRIAVRECADGLAREPSLEHIVFACFDENML
                     KHYQTELGKL"
     gene            complement(2153..>2673)
                     /locus_tag="VFY80_01590"
     CDS             complement(2153..>2673)
                     /locus_tag="VFY80_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX6006623.1"
                     /translation="MYGREVIWRPILLGAVFKLTGQQPLPSIPLKGNYAKHDLDRTAR
                     LLRVPFRFPNRFPIGAQAPSRAFYYMHDRDRDMAVRLAQALYRAYFAEDRDISSPEVT
                     ASVASTLGLDREALLAALGDAAVKDRLRSEVDAAIARGVFGSPYIVIDGEPFWGADRL
                     DQVEKWLATGGW"
BASE COUNT          438 a          902 c          886 g          447 t
ORIGIN      
        1 gccacctcat gcagtcaccc tcccccgatc tacatcctga acacgccgaa gcgcgtctcg
       61 gggatcggcg cgtggagcgc tgcctcgatc ccgagcgcaa ggatgcgccg cgtgtcgagc
      121 gggtcgatca ccccgtcgtc ccacaggcgg gccgtggcgt agtacgggtt gccctcgcgc
      181 tcgtacgcgt cgaggatcgg cgcctcgaac gcgttgcgct cggcgtcgct cgcgaattcg
      241 ccccggaccg tcgacaggac ccgcgccgcc tgctgtccgc ccatcacgct gatccgcgcg
      301 ttcggccagg tccacaggaa ccgcggctga tacgcgcggc cggccatcgc gtagttgccg
      361 gccccgaagc tgccgccgac gatcaccgtg aacttcggca cctcggcgct cgcgaccgcg
      421 gtcacgagct tcgccccgtc ctttgcgatg ccgcccgcct cgtacttcga gccgaccatg
      481 aaaccgctga tgttctgcag gaagacgagc ggggtgccgc gctggcaaca gagctgaatg
      541 aagtgcgtcg ccttgagtgc cgattccgaa aacaggatgc cgttgtttgc cagtatgccc
      601 accggataac catcgatgcg cgcgaaaccg cacaccaagg tcgtgccgta acgcgccttg
      661 aactcgtcga actcgcttcc gtccaccacg cgggcgatga tttcacgcac gtcgaaaggc
      721 acgcgcggat tcaccggcat gatgccgtac agttcctcag gatcgtaggc gggcggctgc
      781 ggcgtggcgc gtctcatatc ggccgccggg gcgggattaa cgtgagccag cgcacgccgt
      841 gcgatctcga gggcatgacg gtcgtcgtcg gcgagatggt cggccacgcc ggaaacacgc
      901 gtgtgaacgt cgccgccgcc gagcgcctcc gcggtcaccg tttcaccggt tgcggccttc
      961 acgaggggcg gccccgccag aaagatggtc ccctggccgc gcacgatgat cgtctcgtcc
     1021 gacagcgcgg gcacataggc gccgcccgca gtgcacgagc ccattacgac cgcgatctgc
     1081 gggataccgc gcgcggaaag ttgcgcctga ttgtagaaga tgcgtccgaa gtgctcgcga
     1141 tcggggaaga cctcgtcctg ttgaggcagg tatgcgccgc cggaatccac gaggtagatg
     1201 cacggcaggc ggttttcgag cgcgatctgc tgcgcgcgca ggtgcttttt cacggtgagc
     1261 gggtagtagg ttccgccttt gacggtcgca tcattcgcga ttacgacgca atgacggccg
     1321 gatacctcgc cgacaccggt gatgataccg gcagcgggca ccggttcatc gtatacgtcc
     1381 cacgccgcga gcggcgagag ttcgagccat ggcgcgccag tgtcgacaag tgccgcgatg
     1441 cgctcgcgag ccggcagttt gccccgtgcg caatgcctgg cgatcgccgc ttcgccgccg
     1501 ccctcgcgcg cccgcgcaag ccgcgcacgc agatcgtcaa cctgcgcgcg catctgccgc
     1561 gcggcggtct cgaattcggg cgcgttcggc cggatgcgcg atcggatgag gggcatcgtt
     1621 cagagcttgc cgagctcggt ctgatagtgt ttcagcatgt tctcgtcgaa gcaggcgaac
     1681 acgatgtgct cgaggctcgg ctcgcgcgcg agaccgtccg cgcattcgcg caccgcgatc
     1741 ctcaccgcgt cggcaatcgg gtaacggtac gcgccgcaac tgatggcggg gaaggccagg
     1801 gtcttcaccc cgtgctcaac ggcgagggcg aggctcgagc gatagcacga ggcgagcagt
     1861 tccggctcgc gcttgctgcc gccatgccat accggtccca cggcatggat gacgtatttc
     1921 gcgggtaggc ggtagccacg cgtgatgcgg gcttcccccg tcgggcagcc gccgatggcg
     1981 cggcacgcct gcagcagctc agggcccgcg gcacgatgga tcgcgccgtc cacgccgccg
     2041 ccgccaagta gcgacgtatt agcggcgttc acgatggcgt ctacgttcag ctcgacgata
     2101 ttggccagtc gggcttcgag cttgtccagc atgatgacct ccggtacggg cgttaccagc
     2161 cgccggtcgc gagccacttc tcgacctggt cgagacggtc cgcgccccag aacggttcgc
     2221 catcgatcac aatgtagggc gagccgaaca cacctcgcgc gatcgccgcg tccacttcgc
     2281 tcctgagccg atccttgacc gcggcgtcgc cgagcgcggc gaggagggcc tcgcgatcaa
     2341 gtcccagtgt ggaagcgacg ctcgctgtaa cctccgggct cgagatatcg cggtcctccg
     2401 cgaagtaggc ccgatacagc gcctgtgcga gcctgaccgc catgtcgcgg tctcgatcgt
     2461 gcatgtaata gaaagcgcgg ctgggcgctt gtgcgccgat gggaaaccgg ttcgggaacc
     2521 ggaacggcac gcgcagcagg cgcgccgtgc ggtcgaggtc gtgctttgcg tagttgccct
     2581 tgagcgggat cgagggcagc ggctgctggc ccgtgagctt gaaaaccgcg cccagcagaa
     2641 tcggccgcca gatcacttcc ctaccgtaca ttg
//
LOCUS       DASURG010000096         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_223832, whole genome shotgun sequence.
ACCESSION   DASURG010000096 DASURG010000000
VERSION     DASURG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223832"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFY80_01595"
     CDS             complement(<1..561)
                     /locus_tag="VFY80_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006624.1"
                     /translation="MHPLTVYTKTAKGVLETRSKTGRLSRELNRVFLMVDGKSTVSEM
                     FAKDGGLEESKLQDALAKLEADGYIKVFSAPAEAPKPRPVEIPRVEPEEDLELDFTSA
                     DVVARLNQEAAARARAESEAKARAEVAAQALAQAKAKQESEARARAMAETRMRAEAEA
                     KAKAEAQARAAAEARAKAEAEARVAAN"
     gene            complement(753..1757)
                     /locus_tag="VFY80_01600"
     CDS             complement(753..1757)
                     /locus_tag="VFY80_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX6006625.1"
                     /translation="MSSKLPATMNVIEMTRAGGPEVLKLAQRPVPGVKPDEVLIEVEA
                     TGVNGPDLMQRKGLYPAPAGASDLLGLEVSGKIVAAGAEVRRWKSGDHVAALTNGGGY
                     AQYCVVDAEHCLPVPQGVSVRDAAGLPETFFTVWSNLFIGVGLKAGETLLVHGGAGGI
                     GTTAIQLGKAFGCKVIATDSPDSRCALCHELGADRVVDYRQEDFVDVVRKEGGANVIL
                     DIVGGPNVERNFKAAAHDARIVQLAFALGSKVDINLMPLMLKRLTYTGSTLRTRPPAF
                     KARTARELEEKVWPEIAHGRIRVVTQHTFPLEEAARAHALMESSQHTGKILLLVKKPA
                     "
     gene            complement(1783..2508)
                     /locus_tag="VFY80_01605"
     CDS             complement(1783..2508)
                     /locus_tag="VFY80_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6006626.1"
                     /translation="MNDELIRKHEGHVTHLTLNRPQKANALSASLVEALLNAVEYAYT
                     DGTRLMLLDGAGAHFCSGFDFTDYQAMSEGELALRFLRIETLLQQLYHAPFETLALAH
                     GRVFGAGADLVCACGVRVAAPGTTFRLPGLRFGVVLGTRRLAQRIGADHARAILHATR
                     TFDADEAQALNFLTHLVSQPQWPDFAARMRAECELLTPSAAAALHRQTVIDSRADDMA
                     ALAASVSAPGLKERIRRYREANP"
     gene            2628..3023
                     /locus_tag="VFY80_01610"
     CDS             2628..3023
                     /locus_tag="VFY80_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006627.1"
                     /translation="MAHRLCARDGEHYLSPRVVVARLEAEFPYVEVDEQDGRRHVRGI
                     IRQLERIGDMGLVPIDEAYLQRLKKAEWGSIFVYFGDDPGSEIACLSTAVIPGEALYF
                     VYTSRRHQEAAWPLLLRCAAVLGYEIVQA"
BASE COUNT          505 a          982 c         1026 g          592 t
ORIGIN      
        1 gttggccgct acgcgcgctt cggcttcagc cttcgcacgc gcctccgccg ccgcgcgcgc
       61 ctgggcctcc gcttttgcct tggcttccgc ttccgcgcgc atgcgcgttt ccgccatggc
      121 gcgcgcgcgc gcctcggact cctgcttcgc cttcgcttgc gcgagcgcct gcgccgccac
      181 ttccgctcgc gccttcgctt cgctttcggc tctggcgcgc gcggcggcct cctgattgag
      241 tcttgcaacg acgtcggctg aggtgaagtc gagttcgaga tcttcctcgg gctccacccg
      301 gggtatctct accggacgtg gttttggcgc ctcggcggga gcggaaaaga ccttgatgta
      361 accgtcggct tccagtttcg cgagcgcgtc ctggagcttg ctctcttcga ggccaccgtc
      421 tttggcgaac atttcggaca cggtagactt gccgtccacc atgagaaaca cgcggttcag
      481 ctcacgcgaa agccggccgg tcttgctcct cgtttcgagg acccccttgg cggtcttggt
      541 atagacggtc agcgggtgca tgaagggcgt gcagaagcgg cggagtcgag caaaagctta
      601 ctcgaccgca cgcggtggtg gcgggatggc ctgccgggca tcggcattgt tttcgctcgt
      661 acgggccctg cagacatcac cgtcgttttt ctcctccctg aacgacaccc cggattatag
      721 ggtgcgtcac ttcccgcggc aaagaacgga agtcacgcag gcttcttgac gaggagcagt
      781 atcttgccgg tgtgctggct cgactccatg agggcgtgcg ctcgggctgc ctcctcgagc
      841 gggaaggtgt gctgggtcac cacgcgaatg cggccatgcg cgatttcggg ccagactttt
      901 tcctcgagtt cgcgtgcggt tcgtgccttg aacgccggcg ggcgcgtgcg cagcgtcgag
      961 ccggtgtatg tgagccgctt cagcatgaga ggcatcaggt tgatgtcaac cttagagccg
     1021 agcgcgaagg cgagctgcac gatacgcgcg tcgtgtgccg ccgccttgaa gttgcgctcg
     1081 acattcggtc cgcccacgat gtcgagaatg acgttggcgc cgccctcctt gcgcacgaca
     1141 tccacgaaat cctcctggcg gtaatcgacc acccggtctg cgcccagctc gtggcagagg
     1201 gcgcaccgcg aatccgggct gtcggtcgca atgaccttgc acccgaacgc tttcccaagc
     1261 tggatcgcgg tggtcccgat gccgccggcg ccgccgtgca cgagcagcgt ctcgcccgct
     1321 ttgaggccga cgccgatgaa cagattgctc caaactgtga aaaaagtctc aggcagcccg
     1381 gctgcatctc gcaccgaaac gccttgaggg acagggaggc agtgctcggc atcgacaacg
     1441 cagtattgcg cataaccccc gccgttggtg agcgctgcca cgtggtcgcc ggacttccag
     1501 cgccggactt ccgcaccggc cgccacgatt ttcccggaaa cttccaggcc cagcaggtcc
     1561 gatgcgccgg ccggcgccgg gtataggcct ttgcgctgca tgaggtccgg gccgttgacg
     1621 ccggtcgcct cgacttcgat tagcacttca tcgggcttca cgccaggtac gggccgttgg
     1681 gcgagtttca gcacctccgg cccgccggcg cgggtcattt cgatcacgtt catcgttgca
     1741 ggcagtttgc tgctcatgct ttcctcgata gattctcgca agctagggat tcgcttcgcg
     1801 atagcggcgg atgcgctcct tcagtcccgg cgcgctcacg gaagcggcga gggccgccat
     1861 gtcgtccgca cgactgtcga tgacggtctg gcgatgcagc gccgcggctg cgctcggcgt
     1921 gagcagctcg cattccgccc gcatgcgggc cgcgaaatcc ggccattgcg gctgtgacac
     1981 gagatgcgtg aggaagttga gggcctgcgc ttcgtcggca tcgaatgtgc gcgttgcatg
     2041 gagaatcgcg cgggcgtggt cggcgccgat gcgctgcgcg agcctacgtg tgcccaggac
     2101 gacgccgaag cgcaggcccg gaagccggaa ggtcgtgccc ggcgcggcga cgcgcacgcc
     2161 gcaggcgcag accaagtcag cgcccgcgcc gaacacccga ccgtgggcca aggcaagtgt
     2221 ctcgaaaggc gcgtgataga gctgctgcag caaagtctcg atgcgcagaa accggagcgc
     2281 gagttcgcct tcgctcatcg cctgatagtc ggtgaaatcg aaaccggagc agaaatgagc
     2341 gcccgcgccg tcgagcagca tgagccgcgt gccgtcggtg taggcatact ccactgcgtt
     2401 cagcagcgct tcgaccagcg aggcagagag cgcattcgct ttttgtggac ggttgagcgt
     2461 gagatgggta acgtggcctt cgtgcttgcg gatcagttcg tcgttcataa agatgcggag
     2521 gccaatgccg ggcggtcgga tatatactag ggcttcgctg cagcgaaggg aagcgaacaa
     2581 gcgcacggta ctgcagcgtc gaggagaccc gctcaacaca acaagctatg gcacatcgct
     2641 tatgcgcccg ggatggcgag cactatctga gcccgcgcgt ggtggtggcg cgactggagg
     2701 ccgaatttcc gtatgtggag gtggacgagc aggatggacg ccgccatgtg cgcggcatca
     2761 tccgccagtt ggagcgcatc ggcgacatgg ggctcgtgcc gatcgacgag gcctacctcc
     2821 agcgcctgaa aaaggcggag tgggggtcga tattcgttta cttcggcgac gatcccggct
     2881 cggagatcgc gtgtctcagc acggcggtga ttccgggaga ggcgctttac ttcgtgtata
     2941 cctcgcgcag gcatcaggag gcggcgtggc cgctgctcct gcgctgcgcg gcagtgctcg
     3001 gctacgagat cgttcaggcg tgatcgcgcc ctttccattg tggtaacgtg acgcgcgcga
     3061 agaattcatt cccggagacg cacatgctgc cgcgccacgc ccgct
//
LOCUS       DASURG010000097         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_836359, whole genome shotgun sequence.
ACCESSION   DASURG010000097 DASURG010000000
VERSION     DASURG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836359"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFY80_01615"
     CDS             complement(<1..382)
                     /locus_tag="VFY80_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6006628.1"
                     /translation="MQPIRRRKSGDVIRIAVDIGGTFTDGLAVLTPGGRIWVAKTLTT
                     PHDPGVAVTTVIADLLRQVAATCNSARPPVVEFVHGTTLVTNTLIERKGASTGLIVTK
                     GTRDVLTIAREVRYELYDLNLEIPR"
     gene            complement(382..1605)
                     /locus_tag="VFY80_01620"
     CDS             complement(382..1605)
                     /locus_tag="VFY80_01620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX6006629.1"
                     /translation="MNESGIPAGFFSPRVGRITVSPSNAGAQRVRELRAQGRDIIGLT
                     IGEPDFETPSHVKEAAIEAMAREETKYTNVDGTPALKEAIRAKFKRDNGLDYEPDQIL
                     VGNGGKQVVFNAMMATVAAGDEIIIPAPYWVSYLDMPLLAEGTPVPVVCRPEDGFKIT
                     PAALEAAITARTKWFVLNSPCNPTGALYSRAELTALAQVLLRHPHVWVLSDDIYEHLL
                     FDGAKFATIAEVEPALYGRTLTVNGVSKTYAMTGWRIGFCGGPKPLVRNMAKIQSQTT
                     TNASSISQAAAVKALNGPQDFVPQRARAFQERRDRVLHLLREAPGIECFRPAGAFYIF
                     ANCSGMLGRRAPQGALLETEQDAVDYLLDAAGVAVVVGAAYGLSPYFRMSIAAPIEDL
                     TEACQRIRRAAGALK"
     gene            complement(1646..>2505)
                     /locus_tag="VFY80_01625"
     CDS             complement(1646..>2505)
                     /locus_tag="VFY80_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006630.1"
                     /translation="AGGSTDSIGRILGQKLTEYMGQQWIIDNRGGATGTIGHAFAAKA
                     APDGYTLLLGTNSTFAIAPHLYRNLPYDSNTAFAPVSLVAVSPQILSVHPSLPVRTVK
                     DLIALARAKPGAIPFSTAGVGATSHLATELLMNLARIKMTHVPYKGGGPSAQALISGE
                     TALSFVDVITALPQAQAGRLRPIATSTAKRTALMPALPTIAESGLPGFESVTSFGMFA
                     PAGTAHEIVMRVNREVVRALAASDVTDKLRAQGIEPAGTAPEELVAHQKRETVKWGKI
                     IREQGIKFE"
BASE COUNT          437 a          768 c          826 g          474 t
ORIGIN      
        1 gccgggggat ctcaaggttg agatcgtaca gctcgtaacg aacctcgcgg gcaatggtga
       61 gcacatcgcg tgtgcctttg gtgacgatga ggccggtgga tgcgcctttg cgctcgatca
      121 gcgtgttggt gacgagcgtc gtgccgtgca cgaattcaac taccgggggc cgtgcgctat
      181 tacacgtggc ggcgacctgc cgcaacagat cggcgatgac agtggtcacg gcaacgcccg
      241 ggtcgtgcgg tgtggtgagc gtctttgcga cccagatacg acccccgggg gtgagtaccg
      301 cgaggccgtc ggtgaacgtc ccgccgatat ccaccgcgat gcgtatcaca tcgccggact
      361 ttctgcgacg gatcggctgc attacttgag cgcaccggcg gcgcgccgta tgcgctggca
      421 tgcctccgtc agatcctcga tgggggcagc gatcgacata cggaagtagg gcgacagtcc
      481 gtacgccgcg ccgacgacga cggcaacgcc tgcagcgtcc agcaggtaat cgacggcatc
      541 ctgttcggtt tcgaggagag cgccctgcgg ggcacgccgc cccagcatgc cgctgcaatt
      601 cgcaaaaatg taaaaggcgc ccgcgggccg gaagcattcg atacccggcg cttcccgcag
      661 caggtggagc acacggtccc ggcgttcctg aaaagcacgc gcgcgctgcg gcacgaaatc
      721 ctgcggcccg ttcagcgctt taaccgcggc cgcctgactg atcgacgagg cgttggtcgt
      781 ggtctgcgac tgtatcttcg ccatgttgcg gacgagcggc ttggggccgc cgcaaaaccc
      841 gatgcgccag ccggtcatcg cgtaggtctt ggaaactccg tttacggtaa gcgtgcgccc
      901 gtagagggca ggttcgacct cggcgatggt ggcgaatttc gcgccgtcaa aaagcagatg
      961 ctcgtagata tcatcggaca ggacccagac gtgcggatga cgcagcagta cttgcgccag
     1021 cgccgtcagt tcagcgcgcg agtagagggc accggtagga ttgcatggcg agttgagcac
     1081 gaaccacttg gtccgtgcgg tgatcgctgc ttccagcgcc gcaggcgtaa ttttgaatcc
     1141 atcttccggg cggcaaacca cgggcaccgg cgtgccctcg gccaagaggg gcatgtcgag
     1201 atacgacacc cagtagggcg ccggaatgat gatctcgtct cccgcggcga ccgtcgccat
     1261 catcgcgttg aacacgacct gctttccgcc gtttcccacc aggatctggt ccggctcgta
     1321 atcgagacca ttgtcacgct tgaacttggc gcgaatcgcc tccttgagcg cgggcgtgcc
     1381 gtccacattc gtgtacttgg tctcctcgcg cgccatggct tcgatcgcgg cttccttgac
     1441 gtgactgggc gtctcgaaat ccggttcacc gatggtgagc ccaatgatgt cgcgcccctg
     1501 ggcgcggagc tcgcgcaccc gctgagcgcc ggcgttgctc ggcgacacgg tgatgcgccc
     1561 gacgcgcggc gagaaaaagc ctgccggaat gccagactcg ttcatcggtc gcccgacaat
     1621 tcttgggata gcgtctttgg cgtgctcatt cgaacttgat tccctgttcg cggatgatct
     1681 tgccccattt cactgtctcc cgcttctggt gagcgacgag ttcctcaggc gcagtgcctg
     1741 cgggctcgat gccttgcgcg cgcagcttgt ccgttacgtc agaggcggcg agtgcacgaa
     1801 cgacctcgcg gttgacgcgc atgacgatct cgtgcgcggt gccggccggc gcgaacatgc
     1861 cgaacgaggt taccgactcg aagcccggaa gacccgattc ggcgatggtc ggcagagccg
     1921 gcatgagtgc cgtgcgcttg gccgtgctgg tcgcaatcgg ccgcaaccgt cccgcttgcg
     1981 cctgcggcag cgcggtgatc acatcgacga aggaaagcgc tgtttccccg gaaatcaacg
     2041 cctgagcgct ggggccgccg cccttgtacg gcacatgggt cattttgatg cgagcgaggt
     2101 tcatcaacag ttcggtagcg agatggctgg tcgccccgac tcccgcagtc gagaaaggga
     2161 tggcgccggg tttggcccgc gcgagcgcaa tcaggtcctt cacggttctt accggaagcg
     2221 aaggatgcac agacaatatc tgcgggctca ctgcgacaag cgagaccggc gcgaacgcgg
     2281 tgttgctgtc atagggaaga ttcctgtaga gatgaggcgc gatcgcgaat gtgctgttgg
     2341 tgccgagcag cagcgtgtaa ccgtccggcg cggcttttgc agcgaacgca tggccgatgg
     2401 tgccggtggc gccgccccgg ttgtcgatga tccactgttg ccccatgtat tcggtgagct
     2461 tctggccgag aatgcgcccg attgagtcgg tcgagccgcc tgccg
//
LOCUS       DASURG010000098         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_427913, whole genome shotgun sequence.
ACCESSION   DASURG010000098 DASURG010000000
VERSION     DASURG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427913"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFY80_01630"
     CDS             <1..744
                     /locus_tag="VFY80_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6006631.1"
                     /translation="ERCADCGFDLVSGLVDIVPTAHYMMGGVVFSADCSTMLPGLFVA
                     GEDSGGVHGANRLGGNGVANSTVFGGIAGDAMAPWVKAHGAFREPDRDALEAAVARCR
                     RPLEKPAGDLNALRERLYDLMWDDVGIMRHQMGLERAAGALDDLEARLDETGVPHADL
                     AFNLTWHDWLNLKNLILVSKAIRFAASAREDSRGAHYREDFPEVRDLENSKYTCVTWS
                     DGRFDMTMGPVRFTRVKPGETLLKEPAVA"
     gene            800..1777
                     /locus_tag="VFY80_01635"
     CDS             800..1777
                     /locus_tag="VFY80_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006632.1"
                     /translation="MKRSMQLARRIATLACVALCPALVWAQDYPSKAVRVLVPFPPGG
                     GSDIIARLVAQKLSIQLGQPAVVENRAGASGNIAAEIVAKSSPDGYTLLFGNSSLSIS
                     PAVYQKLNYSPTRDLTPISMASSYPFALAVHPSLPVKSVRELVALAKARPEQLAYASA
                     GAGTMSHMAMELMRLKTAIKVQHLPYKGAAPASVSLISGETQLAFLVMPVAQSQIKAG
                     KLRGLGVAAPKRSTAAPDIPTMQEAGVEGHIALQWNGFFAPTKTPQPVLDRLYKEVAK
                     AVHLPDVKQRFEAEGATPAGTSPSEFAAFYRAEAEKWADVAKRSGTRLD"
     gene            1949..2947
                     /locus_tag="VFY80_01640"
     CDS             1949..2947
                     /locus_tag="VFY80_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX6006633.1"
                     /translation="MPNVSLEELTALTVRALQKAGASSSMAKATADALIAAESEGLSG
                     HGVSRVALYCQHVREGRADGHARPTLVHEKASACLIEAGGGLAYEAVAMAVQECIARA
                     PRHGIAFVGVRRSHHFGAAAYHLAPIAKARLIGLAFSNSPAAINPWGGKRPLFGTNPI
                     AAIFPRKDAPPIVVDLSLTEVVRGKIMLHAKEGKPIPLGWAVDRDGQPTTDPKAALTG
                     SLAAIGGVKGTMLALMVELICVAMTGAAFGFENDSYFEPGGRPDIGHALIAIDPDALA
                     GRDTYFARVEALVCALLADKGVRLPGSRRRLAAARAREEGVVISDALHQELRALAG"
     gene            3009..>3312
                     /locus_tag="VFY80_01645"
     CDS             3009..>3312
                     /locus_tag="VFY80_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX6006634.1"
                     /translation="MPRSEMTAERECAYREGLGAALRAGYAVLERGGSSLDAVIAAVV
                     VLEDSPLFNAGHGAALNAEGQHELDAAVMDGATWRAGGVALVQRIRNPIVAARAVME"
BASE COUNT          570 a         1077 c         1121 g          544 t
ORIGIN      
        1 gagcgctgcg ccgactgcgg gttcgacctc gtctccgggc tcgtggacat cgttccgacg
       61 gcgcactaca tgatgggcgg ggtggttttc agcgcggatt gcagcacgat gcttccgggc
      121 cttttcgtcg cgggagagga ctccggaggc gtgcatggcg cgaaccgcct cggcggcaat
      181 ggcgtggcga attccaccgt gttcggcgga atcgcggggg acgcgatggc cccgtgggtc
      241 aaggcgcacg gcgctttccg cgagccggac cgcgacgcgc tcgaggcagc cgttgcccgc
      301 tgccgcagac cgttggagaa gccggccggg gacctcaacg cgctgcgcga gcggctctac
      361 gacctcatgt gggatgatgt cggcatcatg cggcaccaga tggggctcga gcgggccgcg
      421 ggcgcgctgg acgatctcga agcgagactc gacgagaccg gcgtgccgca cgcggatctc
      481 gccttcaacc tcacatggca cgattggctc aatctcaaga atctcattct cgtctcgaag
      541 gctatccgat ttgcagcgag cgcacgcgaa gattcgcgcg gcgcgcatta ccgcgaagat
      601 ttccccgaag tacgcgatct cgagaattcg aaatacacgt gcgtcacgtg gagcgacggg
      661 cgtttcgaca tgacgatggg cccggtgcgc ttcactcggg taaagcccgg agaaacgctg
      721 ttgaaagagc cggctgtcgc ctgagagggc gcggtcatcc ggcgcggcag cgcgccacgc
      781 gctacaggag gaggaaacga tgaaacgctc gatgcaattg gcacgccgta tcgcgacgct
      841 cgcttgcgtc gcgctctgcc cggcgctcgt ctgggcgcag gactatccgt cgaaggcggt
      901 gcgcgtgctc gtgccgtttc cgcccggcgg cggcagcgac atcatcgcgc ggctcgtggc
      961 acagaagctc tcgatccagc tcggccagcc ggcagtggtt gaaaatcgcg caggcgcgag
     1021 cggcaacatc gctgcggaaa tcgtcgcgaa atcgtcgccc gacggctata cgctgctctt
     1081 cggcaattcc tcgctctcca tcagtccggc ggtgtaccaa aaactgaact acagcccgac
     1141 acgcgacctt actccgatct cgatggcctc ttcgtatcct ttcgcgcttg ccgtccatcc
     1201 ctcgctcccg gtgaagagcg tacgggagtt ggtcgcgctc gccaaagcca ggcccgaaca
     1261 gctagcgtac gcgtcggccg gtgcaggcac catgtcgcac atggcgatgg agctcatgcg
     1321 cctcaagacc gcgatcaagg tgcagcacct gccctataag ggcgcggccc ccgcctcggt
     1381 gagcctgatc tcgggcgaga cgcagctcgc gtttctcgtg atgccggtcg ctcagtcgca
     1441 gatcaaggct gggaagctgc gcggcctcgg cgtcgcggcg ccgaagcgct ccaccgctgc
     1501 gcccgacatc ccgaccatgc aggaggccgg cgtggaaggc cacatcgcgc tccagtggaa
     1561 cggcttcttt gctccgacga agacgccgca gccggtgctc gaccgcctgt acaaggaagt
     1621 cgcgaaggcg gtgcatctgc cggacgtgaa gcaacgcttc gaagcagaag gcgcaacgcc
     1681 cgcgggtaca tcgcccagcg aattcgcggc gttctatcgc gcggaagccg agaagtgggc
     1741 ggatgtcgcc aagcgctccg gtacccgact cgattagtac tgttccgcaa tctgatcggg
     1801 ttcgcgatgc cggtgaaaac cggcatccag ccacgcatcc ggatgccggc tccggataat
     1861 tcttccggag gttagctttt cgcggccgga atggcgacgg caagatatga gcggtctatt
     1921 gaattgagct cgacccgatg aaaagctgat gccgaacgta tcactcgaag aattgacggc
     1981 gcttacggta cgtgcactgc agaaagcggg cgcgtctagc tcgatggcca aagccaccgc
     2041 ggacgcattg atcgcagccg agagcgaggg cctttccggc cacggcgtct cgcgggtcgc
     2101 gctctattgc cagcacgtgc gcgaaggccg tgccgacgga cacgcgcggc ccacgctcgt
     2161 gcacgagaag gcgagcgcgt gcctgatcga ggccggcggc gggctcgctt acgaagccgt
     2221 cgcgatggcg gtgcaggaat gcatcgcgcg agcgccacgg cacggcatcg ccttcgtcgg
     2281 cgtgcgccgc agccaccatt tcggcgcggc ggcctaccat ctcgcaccca ttgcaaaagc
     2341 gcggctcatc gggcttgcgt tcagcaattc cccggccgcg atcaatccgt ggggcggcaa
     2401 gcgcccgctc ttcggcacca atcccatcgc cgcgatcttt ccgcgcaagg atgcgccgcc
     2461 gatcgtcgtc gatctctcgc tcacggaggt cgtgcgcggc aagatcatgc tgcacgcgaa
     2521 ggaggggaag ccgatcccat tgggctgggc cgtcgatcgc gacggccagc cgacgaccga
     2581 tcccaaagcc gcgctcaccg gaagcctggc ggcgatcggt ggcgtgaagg gcacgatgct
     2641 cgcattgatg gtcgagctca tctgcgtcgc gatgaccggt gccgcgttcg gtttcgagaa
     2701 cgattcgtac ttcgagcccg gcgggagacc agacatcgga cacgcgctca tcgcgatcga
     2761 ccccgatgcg ctggcgggcc gcgatacgta tttcgcgcgc gtcgaagcgc tcgtgtgcgc
     2821 gctgctcgca gacaaaggtg tccgtcttcc cggctcgcgg cgccggctcg ccgccgcccg
     2881 cgcgcgcgaa gagggagtcg tgatttccga tgcgctccat caggagcttc gcgcgctcgc
     2941 gggctgacat cttggcgggc aaatcgaata cgcgcttcgc cctcgccgtg cacggtgggg
     3001 ccggcaccat gcctcgcagc gagatgacgg cggagcgcga gtgcgcctat cgcgaagggc
     3061 tcggcgcagc actgcgcgcg ggctacgccg ttctggagcg cggcggaagc agcctcgacg
     3121 ccgtgattgc ggcggtcgtc gtgctggagg attcgccgct cttcaacgcg ggccacggcg
     3181 cagcactcaa tgccgagggc cagcacgaac tggatgccgc ggtgatggat ggcgcgacgt
     3241 ggcgcgcagg cggcgtcgcg ctcgtgcagc ggatccggaa tccaatcgtc gcggcgcgcg
     3301 cggtgatgga ga
//
LOCUS       DASURG010000099         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1347733, whole genome shotgun sequence.
ACCESSION   DASURG010000099 DASURG010000000
VERSION     DASURG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347733"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..1554)
                     /locus_tag="VFY80_01650"
     CDS             complement(424..1554)
                     /locus_tag="VFY80_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX6006635.1"
                     /translation="MRIVSILALAVVASGASAADWKSVPSPAEYKAMVDLDSVKPQKN
                     FASFTLRRAYRDGQTDPAGNEYFSTRSIVIADCNAGTAVTAVTQFYGEDRKLTHRDVQ
                     KRIAKSEFDTPEAGSDMAEAMKLACARLAESGKDGKAEAAPKSSKPLARSSSGSGIVV
                     SREGLVLTNQHVVRQCDSYEVIDDASRRLKAALLAVDTANDLALLTVREEFPAAALLR
                     KDAAPKLGEAVTVVGYPLVAVLGTRPSVGFGHVAATAGIRGNAKQMQISVPIQRGHSG
                     GPVFDQAGNVIGVVVSKLDALKVAQRIGDLPQNINFAIRGDVVVSFLESQGASFTSSD
                     SPRRLENTEIAARGAAVTVRVRCIRDGAIPAATRSTPATGQQ"
     gene            1670..>2146
                     /locus_tag="VFY80_01655"
     CDS             1670..>2146
                     /locus_tag="VFY80_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006636.1"
                     /translation="MKKKPYASGLYRPHLLAVALVFTFVLSAPAHAECGGLQQCIAIS
                     IDPSVAPRHSTNGINVPAPTLDFGSQAAATASASRTILVAAVEGPAGTRATLNSISLS
                     GTNAGDFTITGGTCTTGSPTLLHDGAAVAQIANACTITVAFRPAAVGVKNAQLNVQT"
BASE COUNT          369 a          698 c          672 g          407 t
ORIGIN      
        1 cctgtactgc tgttcagcct cttcgaattc tccccgttga tgaaattcaa ttccccgctg
       61 gatcgcgagg gctgggtcat attgtgcccg tggtaacttt cctggacgat gcgattcatg
      121 ggcttgctgg accgccccca agaccggagg cgtttgtttt ctgggatgct tagattttgg
      181 catcactttg ggtcatcgtg cagcgaacac ctcgtgcact ttatgacgcc actttacggg
      241 aggtcggatc ccgtcggcaa gataaactgt gccgtccaca ctgtccgcca cgccacaacc
      301 caaagccgat gtggcgacgg atcaagcgaa acggcgcgcg aggaaccttg ttccacgcga
      361 acgagctgcg cgctcggctg ctgcggcgca gagccgtcca ggcggcctga ccggggcgac
      421 gatctactgc tgaccagtcg ccggcgtgga gcgtgttgcc gcggggatgg cgccatcgcg
      481 aatacaccgg acgcgcaccg taaccgccgc gccgcgcgct gcgatttccg tgttctcaag
      541 gcggcgcggg gaatcggatg aggtgaagct ggccccttgg gactctagga acgagaccac
      601 cacgtccccg cggatcgcga agttgatgtt ctgcggcagg tcaccgatgc gctgcgcgac
      661 cttgagcgcg tccagcttcg acacgacgac gccaatcaca tttccggcct gatcgaacac
      721 cgggccgccg ctgtggccgc gctggatcgg caccgagatc tgcatctgtt tggcattgcc
      781 ccggataccg gcggttgccg cgacgtgccc gaagccgacg ctgggacgcg tcccgagcac
      841 cgccacgagc gggtagccga cgacggtcac cgcttcaccc agcttcggcg ccgcgtcctt
      901 tcttaacagc gccgcggcgg gaaactcctc ccgcaccgtc agaagcgcga gatcgtttgc
      961 ggtgtcgacc gcgagcaggg ccgccttcaa ccggcggctc gcgtcgtcga tcacctcgta
     1021 cgagtcgcat tggcgcacca catgctggtt ggtgagcacc aggccctcgc ggctcacgac
     1081 gattccggat ccgctggagc tcctcgcgag cggtttgctg cttttgggcg cggcttccgc
     1141 tttgccatcc ttcccggatt ctgcgaggcg cgcgcaggcg agcttcattg cctccgccat
     1201 gtcggagccg gcctcgggcg tatcgaactc ggacttcgcg atcctcttct ggacgtcccg
     1261 gtgcgtgagc ttgcgatctt cgccgtaaaa ctgcgtgacc gccgtgacag ccgtaccggc
     1321 attgcagtcg gcgatgacga ttgagcgggt gctgaagtac tcgttacctg ccggatcggt
     1381 ctggccgtcg cggtaggcgc gacgcaaagt aaagctcgca aaattcttct gcggcttgac
     1441 ggaatcgagg tcgaccatcg ccttgtattc cgcaggcgag ggcaccgact tccagtccgc
     1501 cgcggaggcc ccgctggcga cgacggccag cgcaagaata gacactatac gcatgttctc
     1561 ctcgctgcag ggcgagctca aagttctccg tattctaaag agtaatgtaa gatactgctc
     1621 gcacgagggg cgggacgcac accgcgccgg tgcgcaggga ggggaagcag tgaagaagaa
     1681 gccttatgca tcgggactgt atcgcccgca tcttctcgcg gttgcgctcg tattcacgtt
     1741 cgtcctctcg gcgccggccc atgccgagtg tgggggcctc cagcagtgca tcgccatttc
     1801 gatcgatccc tcggtcgctc cccgccattc gaccaacggg atcaatgtgc ccgcgccgac
     1861 gctcgacttc ggcagccagg ccgcggcgac ggccagcgct tcgcgaacca tactcgtggc
     1921 agcggtcgaa ggtccggccg ggacccgtgc aacgctgaac tcgatttcgc taagcggcac
     1981 aaacgcgggc gatttcacga tcaccggggg gacctgtacc acgggctcgc ctacgctcct
     2041 gcatgatgga gcggcggtcg cgcaaattgc gaatgcctgc accatcacgg tggcgttcag
     2101 gccggctgcg gtcggcgtga agaatgccca gctcaacgtc cagacc
//
LOCUS       DASURG010000100         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1040928, whole genome shotgun sequence.
ACCESSION   DASURG010000100 DASURG010000000
VERSION     DASURG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040928"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFY80_01660"
     CDS             complement(<1..759)
                     /locus_tag="VFY80_01660"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX6006637.1"
                     /translation="MLQFEGQLRTGAGARVAPQPAADAGKPVEQSRVNIADKRIINGH
                     TDVNQLVPFKYKWAWEKYLSACANHWMPQEIQMSRDIELWRNPNGLTEDERRIVKRNL
                     GFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLGLDEGE
                     VFNAYHEIPSIRDKDEFLIPFIDVLTNPEFKTGTVEADQTLLRSLIVFACLMEGLFFY
                     VGFTQILALGRQNKMMGAAEQYQYILRDESMHCNF"
     gene            complement(817..>3312)
                     /locus_tag="VFY80_01665"
     CDS             complement(817..>3312)
                     /locus_tag="VFY80_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX6006638.1"
                     /translation="EERAKERARQRESQQAARPAQNVINVTENGVAKPLDLAKLTALV
                     ESACQGLGREANPEPVLQAMQRDLYEGVPIEEVRKSLVLAARALIEQDPSYSYVTARL
                     LLHTLRLETLGEEVTQAGMATRYAQYLPEFVKKGIAAELLDERLAQYDLQCLGAALDA
                     NRDLKFTYLGLQILYDRYFLHIHGRRIELPQIFFMRVAMGLALKEPEGTREARAIEFY
                     NILSSFDFMSSTPTLFNSGTRRSQLASCYLTTVSDDLDGIYEAIKENAMLQKYAGGIG
                     NDWTPVRALGAYIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLETWHLDI
                     EEFLELRKNTGDDRRRTHDMNTANWVPDLFMKRVMEEGEWTLFSPSDVPDLHDKYGRA
                     FERAYIEYEAKAARGELKLYKKVPAVQLWRKMLSMLFETGHPWLTFKDPCNIRSPQQH
                     VGVVHSSNLCTEITLNTGPEEIAVCNLGSINMPAHLDLATGRMDMEKLKRTVGTAMRM
                     LDNVIDLNFYNVNKARNSNFKHRPVGLGIMGFQDCLHLLRVPYGSPAAVDFADRSMEA
                     IAYTAYWASTDLAEERGPYSTFRGSLWERGILPFDTLQMLAEERGAYVDCDMSTTLDW
                     EALRKRIGQVGMRNSNCLAIAPTATIANITGLSQCIEPTYQNLYVKSNLSGEFTVANE
                     FLVRDLKKLNLWDEVMIADLKYFDGSLAQIDRIPPDIRSLYATAFEIEPKWLVECAAR
                     RQKWVDQSQSLNIYMAGASGKRLDETYKLAWLRGLKTTYYLRTLGATSAEKSTAKAGQ
                     LNAVPATGGMVAATQSAEPKFCSIDDPECEACQ"
BASE COUNT          539 a         1029 c         1040 g          704 t
ORIGIN      
        1 gaaattgcag tgcatggact cgtcgcgcag gatgtattga tactgctctg ctgcgcccat
       61 catcttgttc tgccgcccga gtgcaagaat ctgcgtgaat ccgacataga agaacagtcc
      121 ttccatcagg catgcaaaca caatcagcga acgcagtagc gtctggtctg cctcgaccgt
      181 tccggttttg aattccgggt tggtcagcac gtcgatgaag ggaatcagga actcgtcctt
      241 gtcgcggatc gacgggatct cgtgataggc gttgaacacc tcgccctcgt cgagccccag
      301 gctctcgacg atgtactgat acgcgtgcgt gtggatcgcc tcctcgaacg cctgccgcag
      361 cagatactga cggcattcgg gcgccgtgat atggcggtag gtgccgagca cgatgttgtt
      421 cgcggcgagc gaatcggcgg tgacgaagaa gcccagattg cgcttgacga tgcgccgctc
      481 gtcttcggtg agcccgttcg gattgcgcca cagctcgatg tcgcggctca tctggatctc
      541 ctgtggcatc cagtggttcg cgcacgccga gagatatttc tcccaggccc acttgtactt
      601 gaacggcacg agctgattga cgtcggtgtg gccgttgatg atgcgcttgt ccgcgatgtt
      661 gacgcggctc tgctcgaccg gtttcccggc gtccgcggca ggctgtggcg ccacccgcgc
      721 gccggcgccc gttcttagtt gtccttcgaa ttgcagcatg tcatgcgtct ccttgatttg
      781 cctcttccct gattgcctgt aatcccttcg aatcgactac tggcacgcct cgcattccgg
      841 atcgtcgatg gagcagaact tgggttccgc gctctgtgtc gcagccacca tgccgccggt
      901 cgccggcacc gcgttcaact gtcccgcctt cgcagtcgat ttctccgccg aagtcgcgcc
      961 cagcgtgcgc aggtagtagg tcgtcttgag tccgcgcagc catgcgagct tgtaggtctc
     1021 gtcgagccgc ttgcccgacg cgcccgccat gtagatgttg agcgactgcg actggtcgac
     1081 ccacttctgc cgccgcgccg cgcattcgac cagccacttc ggctcgattt cgaacgcggt
     1141 cgcatagagg ctcctgatgt cgggcggaat gcggtcgatc tgcgcgagcg agccgtcgaa
     1201 gtacttcaga tccgcgatca tcacctcgtc ccacaggttg agcttcttga gatcgcggac
     1261 gaggaactcg ttcgccacgg tgaattcgcc cgagaggttc gacttgacgt agaggttctg
     1321 gtaggtgggc tcgatgcatt gcgagagccc ggtgatgttc gcgatggtcg cggtcggcgc
     1381 gatcgcgagg cagttcgagt tgcgcatgcc gacctgtccg atgcgctttc tgagcgcttc
     1441 ccaatcgagc gtcgtggaca tgtcgcaatc gacataggcg ccgcgctcct cggcgagcat
     1501 ctggagcgta tcgaacggca ggatgccacg ctcccagagc gagcccctga acgtcgagta
     1561 cgggccgcgt tcctccgcga gatccgtcga agcccaatag gcggtatagg cgatcgcttc
     1621 catcgagcgg tccgcgaagt ccaccgcggc gggagagccg tagggaacgc gcagcagatg
     1681 caggcaatcc tggaagccca tgatgccgag gcccaccggg cgatgcttga agttcgaatt
     1741 gcgggccttg ttgacgttgt agaagttcaa gtcgatcacg ttgtcgagca tgcgcatcgc
     1801 ggtcccgacc gtgcgtttga gcttctccat gtccatcctg ccggtggcga gatcgaggtg
     1861 cgccggcatg ttgatggagc ccaagttgca caccgcaatc tcttccgggc cggtgttgag
     1921 cgtgatttcg gtgcagaggt tggagctgtg caccacgccg acgtgctgct gggggctgcg
     1981 gatgttgcac ggatccttga acgtgagcca ggggtgcccg gtctcgaaca gcatcgagag
     2041 catcttgcgc cagagctgca ccgccggaac cttcttgtag agcttgagtt ccccgcgcgc
     2101 ggccttcgct tcgtactcga tgtatgcgcg ctcgaacgct cttccgtact tgtcgtgcaa
     2161 atccggcacg tcggacggcg aaaagagcgt ccattcgccc tcttccatga cgcgcttcat
     2221 gaaaagatcc ggcacccagt tcgccgtgtt catgtcgtgg gtgcgacggc ggtcgtcgcc
     2281 cgtgttcttg cggagctcga ggaattcctc gatgtcgagg tgccaggtct cgaggtacga
     2341 gcataccgcg cccttcctct tgccgccctg gttcaccgcg accgcagtgt cgttcaccac
     2401 cttgaggaac ggcacgacgc cctggctctt gccgttcgtg cccttgatgt aagcgccgag
     2461 cgcgcgcacc ggcgtccagt cgttgccgat gccgccggcg tatttctgca gcatcgcgtt
     2521 ttccttgatc gcctcgtaga tgccgtcgag gtcgtcggac actgtcgtga gatagcagct
     2581 cgcaagctgc gagcgccgcg tgccggaatt gaacagcgtc ggcgtcgagc tcatgaagtc
     2641 gaacgacgag aggatgttgt agaactcgat cgcgcgcgct tcgcgggtgc cctccggctc
     2701 tttcagcgcg agccccatcg cgacgcgcat gaaaaagatc tgcggcagct cgatgcggcg
     2761 cccgtggatg tgcaggaagt agcggtcgta gaggatctgc agtccgaggt aggtgaattt
     2821 caggtcgcga ttcgcgtcga gcgcggcgcc caggcattgc aggtcgtact gcgccagccg
     2881 ctcgtccaga agctccgccg cgatcccttt cttcacgaat tcggggaggt actgcgcata
     2941 gcgcgtcgcc atccccgcct gcgtcacttc ctcaccgagc gtctcgagcc gcagcgtgtg
     3001 caggaggagc cgggcggtca cgtagctgta gctcgggtcc tgctcgatca aagcacgcgc
     3061 ggcgagaacg agcgatttgc gcacttcctc gattggcacg ccttcgtaga ggtcgcgctg
     3121 catcgcctgc agcaccggct cgggattcgc ctcgcgcccg agcccctggc acgcgctttc
     3181 gacgagcgcc gtcaacttcg cgagatcgag cggctttgcg acgccgttct ccgtcacgtt
     3241 gatgacgttc tgggcgggac gggccgcctg ttgcgattcg cgctgccggg cgcgctcctt
     3301 cgcgcgttcc tc
//
LOCUS       DASURG010000101         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_939472, whole genome shotgun sequence.
ACCESSION   DASURG010000101 DASURG010000000
VERSION     DASURG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939472"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VFY80_01670"
     CDS             complement(<1..1944)
                     /locus_tag="VFY80_01670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6006639.1"
                     /translation="MDSAIAKHAPHPGRIRKWLRSSLSHRIAAFSLGLALAAAAVVGT
                     GSYVAMHTLLDIAIERELEYQAALVARSLEGAVNVLYRDASRMSTNGALIRAVSQAPV
                     QQDALKAVVGSYTGPAGIPAGVSVHDTAGRPIAVTASMAPRSYENAAWLRRVVNFNFT
                     YGEIANEDGKRLLFIARPVQISSGVTGAILVLRAPVEDIFGRAVSMIFDDYNKRLLSD
                     RGEVLAEMSWRRLERPVRATQSLALDIGAFPGQLLLEISGERDQVLAPLAWLAACYAA
                     AAGIVLVLLLGILRRYGARLTAGLARLTEKAAAVAGSSAPLPGVPVEGEDEIARLANA
                     FNAVCANVNELQQEVESRVEKRTVTLQDMNTALVKEILSHKKTGEQLQVAANALENAA
                     EGVMICDADERIISVNKAFTRITGYTVEEVLGTTPDMLLGDEESRARRAEILRTVVDE
                     EHWRGELWSRNKAGERYLEQCSVTAVKDEVGRLVNFIVLLSDVTKQKEDEMRLQFLAH
                     HDPLTGLVNRTLFQQRCEEALRFAERKQTKAAVVFIDLDHFKTVNDSLGHAYGDDLLR
                     QAASRLAECVRKTDVVGRLGGDEFIILLNEVGDSGDVALIAKKILDRLTASFSVAGHE
                     IFVSASLGISWFPDDGQNAATLIQ"
     gene            2126..>2500
                     /locus_tag="VFY80_01675"
     CDS             2126..>2500
                     /locus_tag="VFY80_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006640.1"
                     /translation="MRVSLRLWNFALVWVSLAVFAGIGMAADFPTRPVRFVVASSPGG
                     ALDVLARLVGPKLTEKWGQPVVVDNRAGAGGVIGTEIVANASPDGYNVLVIAQGFTAN
                     PFLYQKLPYRTPDDFAPITILAY"
BASE COUNT          415 a          812 c          807 g          466 t
ORIGIN      
        1 ctggatgagc gtcgcggcat tctggccgtc atccgggaac cagcttatgc cgaggctcgc
       61 ggagacgaat atctcgtggc cggcgacgga aaacgaggcg gtcagacgat cgaggatctt
      121 cttcgcgatc agcgcgacat cgcccgaatc gccgacttcg ttcagcagga tgatgaattc
      181 gtcaccgccg agccgtccca ccacgtcggt cttgcgcacg cattcggcga ggcggctggc
      241 cgcctgccgc aggaggtcgt cgccatacgc gtgcccgagg gaatcgttta cggtcttgaa
      301 gtggtcgaga tcgatgaaga cgaccgctgc cttggtctgc ttgcgctccg cgaacctcag
      361 ggcctcctcg caacgttgct ggaacagcgt gcggttgacg agaccggtga gcggatcgtg
      421 atgcgcgagg aactgcaggc gcatctcgtc ttccttctgc ttggtcacgt ccgacagcag
      481 cacgatgaaa ttgacgagcc gaccgacctc gtccttgacc gccgtcacgc tgcactgctc
      541 gagatagcgc tcgccggcct tgttgcggct ccagagctca cccctccagt gctcctcgtc
      601 cacgacagtg cgcaggatct cggcgcggcg cgcacgcgat tcttcgtcac cgagcagcat
      661 gtccggcgtc gtgccgagca cttcttccac ggtgtagccg gtgatgcgcg tgaacgcctt
      721 gttgaccgaa atgatccgtt cgtcggcgtc gcagatcatc acgccttccg cggcgttttc
      781 gagcgcgttc gccgcgacct ggagctgttc acccgtcttc ttgtggctca ggatttcctt
      841 gacgagcgcg gtattcatgt cctgcagcgt cacagtgcgc ttctcgaccc gggattccac
      901 ttcctgctgc agctcgttca cgttggcgca aaccgcgttg aatgcgttcg cgagccgggc
      961 aatctcgtct tctccttcca ccggcactcc gggcagcggc gcggaacttc cggcgacggc
     1021 ggccgctttc tcggtcaatc gcgcgagccc ggccgtgaga cgcgcgccat agcgccggag
     1081 gatgccgagc agaagtacca gcacgatgcc cgccgcggcc gcatagcatg cggcgagcca
     1141 cgcgagcggc gcaagcacct gatcgcgttc gcccgagatc tcgagcagga gttgccccgg
     1201 gaatgccccg atgtcgagcg ccagcgactg cgttgcgcgc accgggcgct cgagtcggcg
     1261 ccagctcatt tcagccagga cctcgccgcg gtccgacagc agccgcttgt tgtagtcgtc
     1321 gaatatcatg gagactgccc ggccgaagat gtcttcgacc ggcgcgcgca gcacgagaat
     1381 ggcgcccgtc acacccgatg aaatctgcac gggccgagcg atgaagagca ggcgcttgcc
     1441 gtcttcgttc gcgatttcgc cgtaggtgaa gttgaaattg accacgcggc gcagccaggc
     1501 cgcattctcg taggagcgcg gcgccatgct ggccgtgacg gcgatcggcc ggccggccgt
     1561 gtcgtgcacg cttacgccgg caggaatgcc agcgggcccc gtgtagctgc ccacaaccgc
     1621 ctttaacgcg tcttgttgca caggtgcctg ggacaccgcg cggatgagtg cgccgttggt
     1681 ggacatgcgg ctcgcgtcgc ggtagaggac attcacggcg ccttccaaac tgcgggcaac
     1741 gagcgcggcc tggtactcca gctcgcgctc gattgcgatg tcgagcagcg tatgcatcgc
     1801 gacatacgag ccggttccga cgaccgcggc ggcggcgagc gccaatccca gtgaaaaggc
     1861 ggcaatgcgg tgcgacaggc tcgaccgcaa ccatttccgg atccggcccg ggtgaggggc
     1921 gtgctttgcg atcgcgctat ccatcccgat cccttgttat ggttatccgc gcgaggactg
     1981 cgccgcatag taccgcaaag ttgcggcatc gtggagaaaa atgcgcgcgt ggtcagcatg
     2041 gtcgatgagc gcggtgcagc cggcgcccaa cgtgcatgcg cgaacggcga tatgatggag
     2101 gccactcaat gaggaggcac agccgatgcg cgtttcgcta cgcttgtgga atttcgcgct
     2161 ggtgtgggta agtctcgccg tgttcgccgg tatcggcatg gcggccgatt ttccgacgcg
     2221 gccggtgcgt ttcgtcgtcg cctcgtcacc cggcggcgcg ctcgatgtgc tggcgcggct
     2281 cgtcgggccg aagctcaccg agaagtgggg ccagcctgtc gtggtcgaca accgggcggg
     2341 cgcgggcggc gtgatcggca ccgagatcgt tgcgaacgcc agccccgacg gctataacgt
     2401 cctcgtcatc gcacagggtt ttaccgccaa cccgtttctc taccagaaac ttccgtaccg
     2461 cacgccggac gattttgcac cgatcaccat cctcgcgtac
//
LOCUS       DASURG010000102         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1143680, whole genome shotgun sequence.
ACCESSION   DASURG010000102 DASURG010000000
VERSION     DASURG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143680"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFY80_01680"
     CDS             <1..1118
                     /locus_tag="VFY80_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX6006641.1"
                     /translation="LLSWADRFRRLGVWTNADYPRDAARARKFGAEGIGLCRTEHMFF
                     EAERLPVVQSMILARNAEEREQHLSKLLPMQRADFSGLFEAMNGLPVTIRLIDPPLHE
                     FLPSHDELLREITELETRLRLKDGDAVGLEQALDAKRKLLESVEALREQNPMLGLRGV
                     RLGIHMPELVRMQMRAIMEAACACAKKGIKLKPKIMIPLAATAKELEIQRGVLEEEAQ
                     KVITEQGIKVPYQFGTMIEVPRAALVADEIAEYAEFFSFGTNDLTQTTFGISRDDAET
                     GFLSEYLQKGVLERNPFATIDQKGVGQLMEIGVKLGRQRRKGLEIGICGEHGGDPASI
                     AFCHRAGLDYVSCSPFRVPVARLAAAHAALEGATDER"
     gene            1149..>2034
                     /locus_tag="VFY80_01685"
     CDS             1149..>2034
                     /locus_tag="VFY80_01685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6006642.1"
                     /translation="MPQRFVAHLDMDAFYASVELLRYPELRGKPVVIGGRRRPTAGDA
                     FTRLRDYAGRGVVTTAPYEARTFGVHSGMGLMKAARLIPDAILLPADFDEYRRYSALF
                     KEAVRALAPHIEDRGIDEVYIELTELVTARAEEGPPDPWARARTVALDIQRAVRDATR
                     LSCSIGITRNKLLSKIASDLDKPGGVTVLTEADIPVRVWSLPVRRVNGIGPKAAARLE
                     GLGIRTIGELAKADSEFLVAHFGTVYGRWMHDAAHGRDERPVMAVSTPKSLSRETTFE
                     NDLHPVRDREALSQAFTGL"
BASE COUNT          349 a          666 c          690 g          329 t
ORIGIN      
        1 agttgctgtc gtgggcggat cgcttcagac ggctcggcgt gtggaccaac gcggattacc
       61 cgcgtgatgc agcgcgcgcg cgcaaattcg gcgcggaagg catcgggctc tgccgcaccg
      121 agcacatgtt cttcgaggcc gaacggctgc cggtggtgca gagcatgatc ctcgcgcgca
      181 acgccgaaga gcgcgagcag cacctatcga agctgctgcc catgcagcgc gcggatttca
      241 gcggactgtt cgaagcgatg aacgggctac cggtgacgat ccgcctgatc gatccgccgc
      301 tgcacgaatt cctcccgagc cacgacgagc tcctgcgcga gatcacggag ctcgaaacgc
      361 ggcttaggct caaggacgga gatgccgtcg ggctcgagca ggcgctcgat gcgaagcgca
      421 agctactcga gagcgtggaa gcgctacgcg agcagaatcc gatgctcggg ctgcgcggcg
      481 tgcgcctcgg catacacatg cccgagcttg tgcgcatgca gatgcgcgcg atcatggaag
      541 cggcgtgcgc ctgcgcgaag aagggtatca agctgaagcc caagatcatg atcccgctcg
      601 cggcgactgc gaaggagctg gaaatccagc gcggcgtgct ggaggaggag gcgcagaagg
      661 tgatcacgga gcagggcatc aaggtgcctt accagttcgg cacgatgatc gaggtgccgc
      721 gcgcggcgct cgtcgcggat gagatcgccg agtatgccga gttcttctcg ttcggcacga
      781 acgatctcac ccagacgacc ttcggcatct cgcgcgatga cgccgaaacc ggcttcctga
      841 gcgagtacct gcaaaagggc gttctcgagc gcaacccgtt cgccaccatc gatcagaaag
      901 gtgtgggtca gctgatggaa atcggtgtga agctcggccg ccagcggcgc aagggcctcg
      961 agatcggcat ctgcggcgag cacggcggag atcccgcgag catcgccttc tgccatcggg
     1021 ccgggctcga ttacgtctcg tgttcgcctt tccgcgtgcc ggtcgcgcga ctcgctgcgg
     1081 cccatgctgc gctggaaggc gccacggacg agcgttaacg ccggcatgcc ggcgtggccg
     1141 gaagcccgat gccccagcgc ttcgtcgctc atctcgacat ggatgcgttc tacgcgtccg
     1201 tcgagcttct gcgctatccc gagctgcgcg gcaagcctgt cgtcatcggc gggcggcgcc
     1261 ggcccaccgc gggcgatgcg ttcacccgat tgcgggatta cgccggccgc ggtgtcgtga
     1321 cgaccgcacc ctatgaggcc cgcacgttcg gcgtgcactc cggcatgggc ctcatgaagg
     1381 ccgcgcggct catcccggat gcgatcctgc tgcccgccga cttcgacgag taccgccgct
     1441 actccgccct cttcaaggag gccgtgcgcg cactcgcgcc gcacatcgag gatcgcggca
     1501 tcgacgaggt ttacatcgag ctcaccgagc tcgtgaccgc gcgcgccgaa gaaggtccgc
     1561 ccgatccgtg ggcgcgggcg cgcaccgtcg cgctcgacat ccagcgcgca gtgcgcgatg
     1621 cgacccgcct ctcatgctcg atcgggataa cgcgcaacaa gctgctgtcg aaaattgcgt
     1681 ccgacctcga taagcccggc ggcgtgacgg tgctcaccga ggcggacatc cccgtccgcg
     1741 tctggtcgct gccggtgcgc cgcgtgaacg gcatcgggcc caaagcggct gcgcggctcg
     1801 agggtctcgg catccgcacg atcggagagc tggcgaaggc ggattctgaa tttctcgttg
     1861 cgcacttcgg cacggtctac gggcgttgga tgcatgacgc cgcgcatggc cgcgacgagc
     1921 ggccggtcat ggcggtcagt acgccgaagt cgctgagccg cgagacgact ttcgagaacg
     1981 acctgcatcc ggtgcgcgac cgggaggcgc tttcgcaggc cttcaccggg ctct
//
LOCUS       DASURG010000103         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_327043, whole genome shotgun sequence.
ACCESSION   DASURG010000103 DASURG010000000
VERSION     DASURG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327043"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1104
                     /locus_tag="VFY80_01690"
     CDS             130..1104
                     /locus_tag="VFY80_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX6006643.1"
                     /translation="MAGILAIVEQRDGALRKVSEEVVAAAALVGEKLGGEVHALLAGP
                     TGVASAAGALGKFGAAKVFVAEHDALKQYDPDALSKLIADHIRKNNYDVVIFGGSSFG
                     KDLAPRVAAKLDVPLATDATAIEVEGTDLIVTRPVYAGRAIAKVSIDATPRIVSVRPN
                     SFRPQARNAAGTVEQLAVDGIGAARLKIREVKAAGAAKVDVAEAPIVVSGGRGLKGPE
                     NWHLLEGLRDALGSSCALGASRAVVDAGWRPHAEQVGQTGKTVSPQLYFAVGISGAMQ
                     HLAGMRSARTIVAINKDPDAPIFKVADYGIVGDATEVLPKLVEEIKKG"
     gene            complement(1224..1811)
                     /locus_tag="VFY80_01695"
     CDS             complement(1224..1811)
                     /locus_tag="VFY80_01695"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX6006644.1"
                     /translation="MKAKWTGISMSEQVRLLTIFHARKQTLMRSLWLLATCLIWVPAL
                     PAQAQPTTVILVRHAEKEAQPADNPPLSAQGRKRVEALIEAVRQAGVTSIYSTPYART
                     LETARGVAQVLELPVIETPIPNRSVPAYGDSVAARVRRAGGVILVVGHSNTMGAVIKA
                     LGGPEIAEIPDTEYGNLFVLTVEDGQPVRMLRAKF"
     gene            complement(1857..>2296)
                     /locus_tag="VFY80_01700"
     CDS             complement(1857..>2296)
                     /locus_tag="VFY80_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6006645.1"
                     /translation="RRNMRLAFKSGFYLGGFKAVLMTLTDGAFPGGFIETPPDAAEPR
                     VDGGHQTFVPDNKLTYRKLDAVFKSGNATRDDIPSHLIVGKDIPREVAEFYTHVCPAG
                     VYELDGDKLTVNAPNCVDCKATDVLGPRWTPREGGSGPKYRRM"
BASE COUNT          425 a          716 c          726 g          429 t
ORIGIN      
        1 tgcgccacga ctcacgctca aatcgctgac gccccctgcc gagcgcgccg ccggcaagat
       61 cgtaggcaat ggtgcggatg cagtgcctga gctggttcgc ctgctcaaag aagaagcgaa
      121 ggtgctctga tggctggcat attggcaatc gtcgagcagc gcgatggtgc gctgcgcaaa
      181 gtctcggaag aagtcgtggc cgccgccgca ctggtgggtg aaaaactcgg tggagaagtg
      241 cacgcgctgc tggccggtcc aaccggcgtg gcgagtgcag ctggtgcact cggcaaattc
      301 ggcgcggcca aggtttttgt cgccgagcat gacgcactca agcaatacga tccggatgcg
      361 ctcagcaagc tgatcgcgga tcacattcgc aagaacaatt acgacgtcgt catcttcggc
      421 ggctcctctt tcggcaaaga cctcgcgccg cgcgtggccg ccaagttgga tgtgccgctg
      481 gcgactgacg cgaccgctat cgaagtcgaa ggcacggatc tgatcgtaac acgcccggtc
      541 tatgccggac gcgccatcgc gaaagtgtcg atcgacgcga cgcctcgcat agtgtctgtg
      601 cgccctaaca gtttccgccc gcaggcgcgc aatgcggccg gcacggtgga acagttggcc
      661 gtcgacggta ttggcgcagc gcgcctcaag atccgcgaag tcaaagctgc gggtgcagcc
      721 aaggtcgacg tcgcagaagc gcccatcgtg gtgagcggcg gacgcgggtt gaagggtccg
      781 gaaaactggc atctgctgga aggtttgcgc gacgcgctgg gctcatcctg cgcactcggt
      841 gcatcgcgtg ccgtcgtcga tgcgggatgg cggccacacg ccgaacaggt cgggcagacc
      901 ggcaagacgg tgtcaccgca gttgtacttc gcggtcggca tcagcggtgc catgcagcac
      961 ctggcaggca tgcgcagcgc acgcaccatc gtcgccatta acaaggatcc ggatgcgccc
     1021 atcttcaagg tcgcggatta cggtattgtt ggtgatgcga cggaagtgct gccgaagctg
     1081 gtggaggaga tcaagaaggg gtagcccgcc ctcgttttcg agccgcgaac cgcccggacg
     1141 ccaggctccc tggcgtccgg gcggttgatt tctttcgttg cactgcgtga gcgcgttctt
     1201 ctcaccatga tgcgctccag ggttcagaac ttcgccctca gcatccgcac cggctgccca
     1261 tcctctaccg tcaacacgaa caagtttcca tactccgtgt ccggaatctc ggcgatctcc
     1321 ggcccgccca gcgccttgat cacggcaccc atcgtgttac tgtgaccgac caccaggatc
     1381 acgccgccgg cgcgccgcac acgggccgct acgctgtcgc cataagcggg gacgctgcgg
     1441 ttcggaatgg gcgtctcgat gaccggcaac tccagcacct gggccacacc gcgcgccgtc
     1501 tcgagcgtcc gcgcataggg cgtcgagtaa atgctcgtga ccccggcctg ccgtactgcc
     1561 tcgatcagcg cctcgacgcg cttgcgaccc tgcgccgaca gcggcgggtt gtccgcaggc
     1621 tgtgcttcct tctcggcatg ccgcacgaga atgaccgtgg tcggctgcgc ttgggcaggc
     1681 aacgcgggaa cccaaatgag gcatgttgcg aggagccaaa gggaacgcat aagagtctgc
     1741 tttcttgcat ggaagatagt gagcaggcga acctgctcgc tcattgaaat gccggtccac
     1801 ttcgctttca tggcgttctg gcgattgatt gcgtcagcct gatcgtaagc acggtcttac
     1861 attcgccgat acttcggccc gctcccgccc tcgcgcggcg tccagcgcgg accgagtaca
     1921 tccgtggcct tgcaatccac gcagttgggt gcgttcaccg tgagtttatc gccgtccagc
     1981 tcgtagacgc cggccggaca tacatgcgta tagaattccg ccacctcacg cgggatgtcc
     2041 ttgcccacga tcaggtggga gggaatatca tcacgcgtgg cattgcctga tttgaagacc
     2101 gcatcgagct tgcgataggt gagcttgttg tcgggcacga aggtctggtg tccgccatcg
     2161 actctgggtt cggcagcatc gggcggcgtc tcgatgaagc cgcccgggaa cgcaccatcg
     2221 gtcaacgtca tgagcacggc cttgaagccg cccaggtaaa aaccactctt gaaggccagg
     2281 cgcatattgc ggcgtt
//
LOCUS       DASURG010000104         6287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_633426, whole genome shotgun sequence.
ACCESSION   DASURG010000104 DASURG010000000
VERSION     DASURG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633426"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFY80_01705"
     CDS             <1..552
                     /locus_tag="VFY80_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006646.1"
                     /translation="RELIALAKKHPGITCGNSGVGTVTHMTAEKFRLRAGIDVTPVNY
                     KGGATPVIALLGGEIAMVMATIPASIQHVRKGKLVALGLAAAKRSPFLPDIPTLAETV
                     KGDVSGAVISGVLAPAGTPRNVIERLNAEFNRASDTARAKEVYAENAAETLRMSPAEI
                     QQSMERDVKAWAEVVRATGVKAN"
     gene            768..1742
                     /locus_tag="VFY80_01710"
     CDS             768..1742
                     /locus_tag="VFY80_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006647.1"
                     /translation="MKRLTVRIALVSLALWIGGSGPAGAQAYPTKPVRMVAPSSAGGP
                     VDVICRAVMQGMEKTLGQSIVIENRAGAAGLIGTDFVAKAPGDGYTLLFGFSGPLAIV
                     PNLNPNTPYDPTTDLVSITQVATAPYVLLVHPSVPAKNVKQLVALARARPGKMNFSSG
                     GNGTGIHMAGELFNLAANVRILHVPYKGAAPAITALMAGEVDLMFNGLPGAITHIKSG
                     KVRALAVGGETRSPLMPDTPTIAESGLDFKTGGWYGLLAPRGTPRAIVDKVNDALARA
                     VNTPEMKARLTQLAVEPATSTPEAFAKLIAYEKGLWAKVIQKAGIKMK"
     gene            1759..2340
                     /locus_tag="VFY80_01715"
     CDS             1759..2340
                     /locus_tag="VFY80_01715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6006648.1"
                     /translation="MNVRIDPKRAAVLVLDVQNDLVKITPRIRENRVLENIAAVIAAA
                     RRSGVPVIHITASVRADFRDIPRDNPLWDGLRKSRQLIYGTKGAAIHPKVRPLKSELV
                     LNKTCVDPFLTTNLGQALQNHDVNTVILTGLWTNWVVEATARHASDMGYRVFIPRECV
                     ASNTAENHDFAINRILPTICYVVPMDPVLAALQ"
     gene            complement(2394..3395)
                     /locus_tag="VFY80_01720"
     CDS             complement(2394..3395)
                     /locus_tag="VFY80_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6006649.1"
                     /translation="MARPQTIDTHAHFFPESYIRLIAEHGKRCGAAVVTDASGATFIQ
                     VGLLLRTGPITPPFMDFNERISFMDRRGIGMHALSLTQPMVYWADDALAVQLCVAFND
                     AVSAAHRAHPDRFIGFACLPFQNPHLALEELERAAKLPGIRGVYMATAVRDRELSDRS
                     FFAVYERIADLGLPIFLHPMMINNERLKQYYLINTIGNPTDTAIAASHLIFGGVLDAF
                     PKLEVSLPHAGGTLPILRGRLDQGYRIRPECKTIPQPPSAYLKRFTYDTISYSEEIMD
                     ELVRLVGADRIMMGSDYCFDIAYNDPVAIVKGMKSLEAAGREQILWSNAARLLKLDS"
     gene            complement(3434..3901)
                     /locus_tag="VFY80_01725"
     CDS             complement(3434..3901)
                     /locus_tag="VFY80_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX6006650.1"
                     /translation="MRYWLLKSEPSSYSIEDMARERVTAWGGVRNYQARNFMRDQMRV
                     GDRAFFYHSNCEDPGIVGIVEVAAPAHPDETQFDRKSKYYDAASRRDAPRWLNVEVKF
                     VKKTRLIPLEELRAHKRLAGMRILQRGNRLSITPVDPAEWNYIMKLAERRGGA"
     gene            complement(4015..4191)
                     /gene="ssrS"
                     /locus_tag="VFY80_01730"
     ncRNA           complement(4015..4191)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFY80_01730"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4194..4508)
                     /locus_tag="VFY80_01735"
     CDS             complement(4194..4508)
                     /locus_tag="VFY80_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX6006651.1"
                     /translation="MNADSKGLQINIMGREFRVACREDEQKGLLEAVEYLNRKMQDIR
                     SSGKVVGLERIAIMAALNIAHELLTTKVGGGAFDIADFKRKMARMENVLDQVMSDQDE
                     LF"
     gene            complement(4505..4705)
                     /locus_tag="VFY80_01740"
     CDS             complement(4505..4705)
                     /locus_tag="VFY80_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006652.1"
                     /translation="MDAELKSLEEKINQVAEYCQRLRADNQKLRQQLASALDDNQRLE
                     QKIESATGRLENLLNQLPEEDA"
     regulatory      4830..5049
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5165..>6287
                     /locus_tag="VFY80_01745"
     CDS             5165..>6287
                     /locus_tag="VFY80_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX6006653.1"
                     /translation="MAVQGRHALLVALLAAAQSSSASDIPVSRGEPVVVTATRFPERI
                     SDAPVNVTVITEADIRSSTARTVPELLAEQPGVALRDLFGNNAASSTVDVRGFGATAG
                     QNTLILIDGRRAGDIDLSGVQWSAVPLTAIERIEIVRGGGSVLYGDGATGGVINIITR
                     SPLALPSGVSAGARVGSYATQELHASANYSGSRAAFNAGAANFESDGYRANNHNRQAT
                     AQADIRWLMESGEIALKMGADRQGIRLPGARTVQPSAGVNQLATDRRGTGTPLDYAQR
                     DGNRATVDWHRSAPFGEFTIGFGWRDKAQRSYFDFGGFPDYRTIDLDVRSFTPRVKLK
                     HALLGTDSALVAGLDWYRWSYRLRRANAPANADRPINTVS"
BASE COUNT         1200 a         1959 c         2003 g         1125 t
ORIGIN      
        1 cgcgagctga tcgcgctggc gaaaaagcac ccgggcatca catgcggcaa ttcgggggtc
       61 ggcaccgtga cgcacatgac ggccgagaag ttcaggctgc gcgcaggtat cgacgtgacg
      121 ccggtcaatt acaagggcgg cgcgacaccg gtcatcgcgc tcctcggcgg tgaaatcgcg
      181 atggtgatgg cgaccattcc cgccagcatc cagcatgtgc gcaagggcaa gctcgtcgcg
      241 ctcgggcttg cagccgccaa gcgctcgccg ttcctgccgg atatcccgac gctcgccgaa
      301 acggtcaaag gggacgtgag cggtgcggtc atctccgggg tgctcgcacc ggccggcacg
      361 ccgcgcaacg tgatcgagcg gctcaacgcg gaattcaacc gcgcgtccga taccgcgcgc
      421 gcaaaggaag tctatgccga gaatgcggcg gaaaccttga ggatgagtcc ggccgaaatc
      481 cagcagtcga tggagcgcga cgtcaaggcg tgggccgagg tcgtccgggc aaccggcgtg
      541 aaggcgaact aaccgtttgt tgaacaatga accgtcacac cggcggaagc cggtgtccag
      601 ataacaggct ggataccggc tttcgccggt atgacgatag agtaataatc gcagccattt
      661 ttcagcgaac ggctagacgg ggagccggtg ttgctgtccg caggaccgtt gtggacacga
      721 gatccggctc aggccaacga agtgccagcg acgaggagga tcgatcaatg aaacgcctta
      781 ctgtcaggat tgccctggtg tcgctcgcgc tctggatcgg cggctccggg cccgccggcg
      841 cgcaggccta tcccaccaaa ccggtgcgca tggtggcgcc gtcctcggcg ggcgggccgg
      901 tggatgtgat ctgccgggcg gtgatgcagg gcatggagaa gacgctgggc cagtcgatcg
      961 tgatcgaaaa tcgcgcgggg gccgcgggcc tgatcggaac cgatttcgtg gcgaaggcgc
     1021 ccggcgatgg ttacacgcta ctcttcggtt tttccgggcc gcttgcgatc gtccccaatt
     1081 tgaatcccaa tacgccctac gatccgacga ccgatcttgt ctccatcacg caggtcgcca
     1141 ccgcccctta cgtgctgctg gtacacccga gcgtgccggc gaagaacgtc aagcagctcg
     1201 tcgcgctcgc gcgcgcgcga cccggcaaga tgaatttctc atccggcggt aacggaaccg
     1261 gcatccacat ggcaggcgag cttttcaacc tcgccgcgaa cgtgcgcatt ctgcacgtgc
     1321 cgtacaaagg cgccgctccc gcgatcacgg ctctcatggc cggggaagtg gacctgatgt
     1381 tcaacggcct gcccggtgcc atcacccaca tcaagagcgg gaaggtgcgt gcgctcgccg
     1441 tcggcggcga aacccgctcg cccctgatgc ccgacacgcc gacgatcgcc gaaagcgggc
     1501 tcgacttcaa gaccggcggc tggtacggcc tgctcgcgcc caggggtaca ccgcgtgcca
     1561 tcgtcgacaa ggtcaacgat gcgctggccc gagcggtcaa cacgccggag atgaaagcac
     1621 gcctgacgca gcttgccgtc gagccggcca cctcgacgcc ggaagccttc gccaagctca
     1681 ttgcctacga gaaaggcctc tgggccaagg tcatccagaa ggccggcatc aagatgaagt
     1741 aatgcgggcg ggaaagcaat gaacgtgcgc atcgacccca aacgcgctgc cgtgctggta
     1801 ctggacgtgc agaacgacct cgtgaagatt acgcctcgca tcagggaaaa ccgcgtgctg
     1861 gaaaacatcg cggccgtgat cgcggccgcc cgccgcagcg gcgttcccgt catccacatc
     1921 acggcttcgg tacgcgcgga ctttcgcgac atcccgcgcg acaaccccct gtgggacggg
     1981 ctgcgcaagt cgcgtcagct gatctacgga acgaaagggg ctgcgatcca tcccaaagtg
     2041 cggccgctca agagcgagct cgtgctgaac aagacctgcg tggacccgtt tctcacgacc
     2101 aacctcgggc aggcgctgca gaatcacgat gtcaacacgg tcatcctgac aggactctgg
     2161 acgaactggg tggtggaggc gaccgcccgg cacgcgagcg acatgggtta ccgcgtattc
     2221 attccgcgcg aatgcgtggc gagcaatacg gcggagaacc acgattttgc gatcaaccgc
     2281 atcctgccca ccatatgcta cgtggtcccg atggaccctg tgctcgccgc gctgcagtga
     2341 ccatcaagcc cgcttagaca ctcttaaggg cgagagtcat ttctctgcgg ggctcaactg
     2401 tcgagcttca ggagccgcgc tgcgttgctc cagagaatct gctcgcgccc cgctgcctcg
     2461 agcgatttca tgcccttcac aatggcgacc ggatcgttgt aggcgatatc gaagcagtag
     2521 tcgctgccca tcatgatgcg atccgcgccg acgagcctga cgagctcatc catgatttcc
     2581 tcggaatagc tgatcgtgtc gtaggtgaag cgcttcaggt acgcgctcgg cggctgcgga
     2641 atcgtcttgc actccgggcg gatcctgtag ccctggtcga gccgcccgcg caggatgggc
     2701 agcgtaccgc ccgcgtgcgg caagctcact tccagcttgg gaaaggcgtc tagcaccccg
     2761 ccgaagatca gatgggaagc ggcaatcgcc gtatcggtgg gattgccgat cgtgttgatc
     2821 agatagtact gcttcagcct ttcgttgttg atcatcatgg ggtgcaggaa aatcggcagc
     2881 ccgagatcgg cgatgcgttc gtagaccgca aagaagctgc ggtcggagag ttcgcgatcg
     2941 cgcactgcgg tcgccatgta gacgcctcgt atgccgggca acttcgcggc gcgctccagt
     3001 tcctctagtg cgagatgcgg gttctggaac ggcaggcacg cgaagccgat gaagcggtcg
     3061 ggatgggcac ggtgcgccgc gctcacggca tcgttgaacg ccacgcagag ctgcacggca
     3121 agcgcgtcgt cggcccagta caccataggt tgggtgagcg agagcgcgtg catgccgata
     3181 ccccgccgat ccatgaagga aatgcgctcg ttgaaatcca tgaagggcgg cgtaatggga
     3241 ccggtgcgca gcagaagccc cacctgtatg aacgtcgcgc ccgacgcatc ggtcacgacc
     3301 gctgcgccgc agcgcttgcc gtgctcggcg atgagccgga tgtacgattc cggaaagaag
     3361 tgggcatgcg tatcgatcgt ctgcggacgg gccattcgcg tactcctccg ttgttttttt
     3421 cgggcgagac gggctacgct ccgccgcggc gttcggcaag cttcatgatg tagttccact
     3481 cggcaggatc caccggcgtg atggagagcc ggttgccgcg ctgcaggatg cgcatgccgg
     3541 cgagccgctt gtgcgcgcgc agttcctcga gcgggatgag ccgcgtcttc ttcacgaact
     3601 tgacttcgac attgagccag cgcggcgcat cgcgcctgga agcggcgtcg tagtacttgc
     3661 tcttgcggtc gaactgcgtc tcatccgggt gcgccggcgc cgccacctcc acgatgccga
     3721 cgatgcccgg gtcctcgcag ttcgaatggt agaagaacgc gcggtcgccg acgcgcatct
     3781 gatcgcgcat gaagttgcgc gcctgatagt tgcgcacacc gccccaggca gtgacgcgct
     3841 cgcgcgccat gtcctcgatg ctgtaggacg agggctcgga cttgagcagc cagtagcgca
     3901 tgacccaaaa agcgaggacg acggccccca aaaaaagcgg gacaaaacgg aacgtcgccc
     3961 gaaacgcaac gacgattgtc aaaaaaaatg cggccgcgcc gcatttctac tgtagggacc
     4021 cccgcatgtg ccgtcaggtc tgcatcttga accggaggtt caaggtggtc gccttccgag
     4081 tcccttcagg cgcttccatg tcggacgcgc acaccggaac tgcttgagtc agcaacctgg
     4141 ttacaaattg gttcagagaa tataagacct aaacggacac agcaggggaa gctttaaaac
     4201 aactcgtcct gatccgacat cacctgatcg agaacgtttt ccatgcgagc cattttacgc
     4261 ttaaagtctg cgatgtcaaa agcgccgccg ccgactttcg tcgtgagcag ttcgtgtgcg
     4321 atgttgagcg cggccatgat cgcgatgcgc tcgagcccga ccactttgcc gctcgagcgg
     4381 atgtcctgca tcttgcggtt gagatactcg acggcttcga gcaagccttt ctgttcgtcc
     4441 tcccggcatg cgacgcgaaa ttcgcgcccc atgatgttga tctgcaggcc ttttgagtcg
     4501 gcattcatgc gtcctcttcg ggaagctggt tgagcagatt ctcgagccgg cccgtcgcgc
     4561 tttcgatttt ctgttcgagc cgctggttgt catcgagcgc ggaagcgagc tgctggcgca
     4621 gcttctgatt gtcggcgcgc agacgctgac agtactccgc gacttggttt attttttctt
     4681 cgagggactt gagctccgcg tccatcgcga cactatagaa gcgaaaattc cggtgcgtca
     4741 actgctaacg tcaaattttg aatgtggttt gtgcggctgc tagaatcgcg ttgacgatgc
     4801 cgcgcgcggg ccattccgca ggccagcgcc ggaatcgcgc ggaagcgggt gcctgccctc
     4861 caagcgaaag gggcgggttg aacgggaaac aggtggaatg cctgtgctgc ccccgcaacg
     4921 gtaagcgaat ggagttcgcc atgggctttt cgcaaggaaa ggcccggcca ctggacgcat
     4981 agcgcccggg aaggcggcga acttcgcaat gcgcgagccc gtataccggc ccggtccgct
     5041 gaacggatgc atgccgcggg gatgcggttc atcgtctgcc ggacccggtt gctcgggcga
     5101 acgactgctc cctgcctgct ccccccttta aacgacgcat gcggcgggca tgcagtgagg
     5161 gaacgtggcg gttcagggac gccatgcgct gttggtcgcg ctgcttgcgg cagcacagtc
     5221 ctcttctgca tcggacattc cggtgagccg gggagagccg gtcgtcgtca cggcgacgcg
     5281 ttttccggag cgtatctccg acgcaccggt gaacgtcacg gtcatcaccg aggccgacat
     5341 ccggtcgagc accgcacgca ctgttccaga gttgctcgca gagcaacccg gcgtcgcgct
     5401 acgcgatctc ttcggcaaca acgcggcctc gtcaacggtg gacgtacggg gtttcggcgc
     5461 aacggcaggc cagaacaccc tcattctgat cgacgggcgg cgggcgggag acatcgatct
     5521 ttccggggtg cagtggtctg cggtgccgct caccgcaatc gagcgtatcg aaatcgttcg
     5581 cggcggcggc agcgtactct atggcgatgg cgcgacgggc ggtgtcatca acatcattac
     5641 ccgttcgccg ctcgcgctgc cgagcggtgt cagcgccggt gcgcgcgtgg gaagctacgc
     5701 gactcaggaa ctccacgcga gtgccaacta ttcgggtagt cgggcagcgt tcaatgcagg
     5761 agcggccaat ttcgagtcgg acggctaccg cgcgaacaac cacaaccgtc aggcgacagc
     5821 gcaggccgat attcgatggc tcatggaatc gggcgagatt gcgctcaaga tgggcgcaga
     5881 ccgacagggc atccgtcttc ccggcgcgcg caccgttcag ccttcagccg gcgtgaacca
     5941 gctcgcaacc gatcgccgcg gcacgggcac gccgctcgac tacgcccagc gcgacggcaa
     6001 tcgcgcaacg gtggactggc atcgcagcgc tccgttcggg gaatttacga tcggcttcgg
     6061 ctggcgcgac aaggcgcagc gctcgtactt cgatttcggt ggctttcccg actaccgtac
     6121 gatcgacctc gatgtgcgct ccttcacgcc ccgcgtaaag ttaaagcacg cattgctggg
     6181 aaccgacagc gcgctcgtcg caggcctcga ctggtaccgg tggtcgtacc ggctgcgacg
     6241 tgccaacgcg ccggcgaacg ccgatcgccc aatcaacacc gtgtcgg
//
LOCUS       DASURG010000105         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_428414, whole genome shotgun sequence.
ACCESSION   DASURG010000105 DASURG010000000
VERSION     DASURG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428414"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2333
                     /gene="hrpA"
                     /locus_tag="VFY80_01750"
     CDS             <1..2333
                     /gene="hrpA"
                     /locus_tag="VFY80_01750"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX6006654.1"
                     /translation="SIQDPRERPMERAAAADKAHEPFQHERSDFLSFLAMWKFFDEAL
                     RHKKSNRKLQDLVREHFLSHRRLREWREVHGQLAALAGEMGMHPNETPAEYAPIHRAL
                     LAGLLGNIGVKTEDGEYAGARGIRFAVFPGSALRKAQPKWVMAAELVETARLYARCVA
                     RMEPEWIEPLARSLVQRHYTDPHWVKDRAMVMAYERVTLYGLTIVARRPVHYGAINPQ
                     EAREIFIRQALAACEYETRVPFLEHNRRLIREVQALEHKARRRDVLVDEATIFAFYDA
                     IVPADIVNGAGFEKWRRGAEEKNPRLLFLTRDYLMRHAASDVTEAQFPECVRVDSADL
                     KLRYRFEPGHPLDGVTVTVPLHMLNKLESARFDWLVPGMVREKVAHAFKSLPKQLRRR
                     FVPVADHVTPFLEAQDRAPQREMTSFSDAVGRYVQRAVGNPVDTALIGRIDYPAHLLM
                     NYRVIDDAGQELATGRDLAALKARLGEAAQLTFSSAPTGIERDGIRAWDFGDLPREIA
                     FTRGGRRLVGYPALVDEEASVAIRLFDVPQAAEVAMRAGVIRLLQLSLKEQVKQLERS
                     LRVLDPAMLQMRAVASVDAMKADVMAAIVDRAFVGEDPLPRTEREFEAQRARARTRLP
                     AVTEAAGRLFTDIATEHQNVAQRLASVRGPLARPAADIKTQLARLVYSGFFSKTPWVQ
                     LAHLPRYMKAMQARLDKYGRDPERDAKHVASLAELWKRYEERLAQQRRAGAIDPRLEA
                     FRWHLEELRVSLYAQELKTPYPVSYKRLDKIWNAIR"
     gene            complement(2421..3263)
                     /gene="rlmJ"
                     /locus_tag="VFY80_01755"
     CDS             complement(2421..3263)
                     /gene="rlmJ"
                     /locus_tag="VFY80_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX6006655.1"
                     /translation="MLAYRHIFHAGNFADVFKHALLVRLLIALSEKDKPYCYLDTHAG
                     IGRYDLTHAWAQKASEFKHGIGRLWERKDIPPVLAPYVDAVKAENTGRKLRFYPGSPL
                     LARRLVRRIDRMVLTELNKTDFAELKQCFARDRQVAVHLLDGYQGLKAFLPPKERRGL
                     VMIDSSFDRAREFDRITTALAEAHKRWATGMYAVWYPLMAPAAMRNFELGVKNLGIRK
                     ILKLEIEVRARDVDEAIPGCGMLVVNPPWRFEDEARPMLRWLWSALSESGAGGATVTW
                     LVPE"
     gene            complement(3263..>3666)
                     /locus_tag="VFY80_01760"
     CDS             complement(3263..>3666)
                     /locus_tag="VFY80_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX6006656.1"
                     /translation="QYTMARLSGHRNNAMKQTIDTLERLRTKLAEFAAARDWDQFHNP
                     KNLAMAVAGEAGELIEHFQWLTFEQSAHLSRATRDEVALECADVLLFLLRLCDKLDID
                     LAAAAHKKLALNAKKYPVEKSRGRATKYTRL"
BASE COUNT          652 a         1166 c         1205 g          643 t
ORIGIN      
        1 tctccataca ggacccgcgg gagcggccga tggagcgcgc ggcggcggcg gacaaggcac
       61 acgagccgtt tcagcacgag cgctcggatt tcctgagctt tctcgcgatg tggaagttct
      121 tcgacgaggc gctcagacac aaaaaatcga accgcaagct gcaggatctc gtgcgcgagc
      181 atttcctttc gcaccgtcgc ctgcgcgagt ggcgcgaagt tcacggccag ctcgccgccc
      241 tcgcgggtga aatgggcatg catcccaacg agacgcctgc cgaatacgcg cctatccatc
      301 gggcgctgct ggcggggctg ctcggcaaca tcggtgtcaa gaccgaagac ggcgaatacg
      361 ccggcgcccg tggcattcgg ttcgcggtct ttcccggctc ggcgctccgc aaggcgcagc
      421 cgaagtgggt gatggctgcg gaactcgtcg aaactgcacg cctttacgcg cgctgcgtgg
      481 cgcggatgga gcccgagtgg atcgagccgc tcgcgcgcag cctcgtacag cgccactata
      541 ccgatccgca ttgggtcaag gatcgggcaa tggtaatggc ttacgagcgg gtgacgctct
      601 acggcctcac catcgtcgcg cggcgcccgg tgcactacgg ggccatcaat ccgcaggagg
      661 cgcgggaaat cttcatccgg caggcgctcg cggcttgcga gtacgaaacg cgcgtgccgt
      721 tcctcgagca caaccgccgg ctgatccgcg aagtgcaggc gctcgagcac aaggcgcgcc
      781 ggcgcgacgt gctggtggac gaggcgacga tattcgcgtt ttacgatgcg atcgtcccgg
      841 cggacatcgt gaacggtgcg ggattcgaga aatggcgccg cggggcggag gagaagaatc
      901 cgaggctcct cttcctcacg cgcgactacc tgatgcgcca cgccgcctcg gacgtcaccg
      961 aagcgcagtt ccccgagtgc gtgcgcgttg acagcgctga tctcaagctg cgctaccgct
     1021 tcgagccggg acacccgctc gatggcgtga ccgtcaccgt gccgctgcac atgctgaaca
     1081 agctcgagtc cgcgcggttc gactggctcg tgcccggcat ggtgcgcgaa aaggtcgcgc
     1141 atgcattcaa gtcgctgccg aaacagcttc gccgccgctt cgtgccggtt gccgatcatg
     1201 tcacgccgtt tctcgaggcg caggaccgtg cgccgcaacg cgagatgaca tcgttctccg
     1261 acgccgtggg acgctacgtg cagcgtgccg tcggcaatcc ggtcgatacg gcgctgatcg
     1321 ggcgcatcga ctatccggcg caccttctga tgaactaccg cgtgatcgac gatgcggggc
     1381 aggaactcgc aaccgggcgg gaccttgcgg cgttgaaggc ccgcctgggc gaggccgcac
     1441 aactgacctt cagcagcgcg ccaaccggca tcgagcgcga cggtatccgc gcctgggatt
     1501 tcggcgatct tccgcgcgag atcgcgttca cccgtggcgg gcgcaggctt gtgggctacc
     1561 cggcgctggt ggacgaggaa gcgagcgtcg cgatccggct cttcgacgtg ccacaggcgg
     1621 cggaagtggc gatgcgtgcc ggcgtcatcc ggctgctgca gctctcgctc aaggaacagg
     1681 tgaagcagct tgaacgcagc ctgcgcgtgc tcgatcccgc catgctgcag atgcgcgctg
     1741 tagcaagcgt ggacgcgatg aaagccgacg tgatggccgc gatcgtcgat cgtgcgttcg
     1801 tcggagagga tccattgccg cgcaccgagc gcgagttcga ggctcagcgc gcgagagcgc
     1861 gtacgcgatt gccggcggtc acagaagcgg cggggcggct gtttaccgac atagccaccg
     1921 agcatcagaa cgtggcgcag cggcttgcaa gcgtgcgcgg accgctggcg cgtccggcgg
     1981 ccgatatcaa aacgcagctt gcgcggctcg tctatagcgg gttcttcagc aagacaccgt
     2041 gggtgcagct tgcgcacctg ccgcgctaca tgaaggcgat gcaggcccgg ctcgacaaat
     2101 acggtcggga cccggagcgc gacgcgaagc acgtggcgag cctcgcggaa ttatggaagc
     2161 gctacgaaga gcggctggcg cagcagcgtc gcgcgggtgc catcgatccg cgtctcgagg
     2221 cgttccgctg gcaccttgag gagctgcgcg tgtcgctcta cgcccaggaa ctgaaaaccc
     2281 cctatccggt ctcgtacaag cgtcttgaca agatctggaa cgcgattcgc tgaactgtaa
     2341 ttccggacaa tgatccggaa tccagacgcc ctttctggat cccggcgtac accgggaaga
     2401 cgatcggcag tgcgacgtag ctactccggg acgagccagg tcaccgtcgc gccgcctgcg
     2461 ccgctctccg acagcgcgct ccacagccag cgcaacatcg gtcgcgcctc atcctcgaag
     2521 cgccacggtg gattgacgac cagcatcccg cagccgggta tcgcctcatc gacgtcgcgc
     2581 gccctgactt cgatctccag cttgaggatt ttgcggatgc cgagattctt gacacccagc
     2641 tcgaagtttc gcattgctgc gggcgccatc agcggatacc agaccgcgta catgccggtc
     2701 gcccagcgct tgtgcgcctc tgcgagcgcg gtggtaatgc gatcgaattc gcgcgcgcgg
     2761 tcgaaggacg aatcgatcat caccagcccg cgacgttctt tcggcggcag gaacgccttc
     2821 agcccctgat agccgtcgag gagatgcacg gcgacctgcc ggtcgcgcgc gaagcattgc
     2881 ttcagctctg cgaagtcagt cttgttgagt tccgtcagca ccatgcggtc gatgcggcgc
     2941 actaatcgcc gcgcgaggag cggcgagccg ggatagaagc gcagcttgcg gcccgtattc
     3001 tcagccttta ccgcgtcaac ataaggcgcc agcacgggcg gaatgtcctt gcgttcccac
     3061 aggcggccga tgccgtgctt gaattcgctc gccttctgcg cccaggcgtg cgtgagatcg
     3121 tagcggccaa tcccggcgtg cgtgtcgaga tagcagtagg gtttgtcctt ttcgctgagc
     3181 gcgatcagca ggcgcacgag cagcgcgtgc ttgaagacat cggcgaagtt gccggcgtga
     3241 aagatatgcc ggtatgcgag cactacagcc tggtgtactt ggttgcgcgg ccgcgggatt
     3301 tctccaccgg atacttcttc gcgttgaggg cgagtttttt gtgcgccgcc gccgcgagat
     3361 cgatgtcgag cttgtcgcac agccgcagca aaaaaagcag cacgtccgcg cactcgagcg
     3421 ccacttcatc gcgcgtcgca cgggaaagat gcgccgactg ctcgaaggtg agccactgga
     3481 agtgctcgat cagttccccc gcttcgccgg caaccgccat ggcgagattc ttaggattat
     3541 ggaactgatc ccagtcgcgc gcggcggcaa attccgcgag cttcgtgcgc aaccgttcca
     3601 gtgtgtcgat ggtctgcttc atggcgttgt tccggtgtcc cgagagacgc gccattgtat
     3661 actgcg
//
LOCUS       DASURG010000106         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1041326, whole genome shotgun sequence.
ACCESSION   DASURG010000106 DASURG010000000
VERSION     DASURG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041326"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..976
                     /gene="corA"
                     /locus_tag="VFY80_01765"
     CDS             11..976
                     /gene="corA"
                     /locus_tag="VFY80_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX6006657.1"
                     /translation="MLVNCVVYHEGRKLADIRKEDIHVYLDRPGCFVWVALKDATLAE
                     LLEMQQQFGLHELAVEDASHGHQRPKLEEYGDALFAVVHTVEEHNGELQVGEVSVFVG
                     RNYVLSIRNRAHEGFADVRARCEREPQHLALGAGFVLYALIDTVVDRYFPVLEYLEDE
                     LDGVERRIFSGTPARQNIEALYQLKQKIMTLRHAAAPLQETASKLHGGRVPEVCMGTQ
                     DYFRDVYDHLVRINQSIENLREMVTTALSVNLSLITLGENETTKRLAAYAALVAVPTM
                     IAGIYGMNFDYMPELRWAFGYPLAVGAMAAIDGYLFYRFRRAKWL"
     gene            1003..1977
                     /locus_tag="VFY80_01770"
     CDS             1003..1977
                     /locus_tag="VFY80_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX6006658.1"
                     /translation="MRAAWYQRNGAAAEVLVVGDLPDPLPAAGEVRVRLYASGVNPSD
                     VKARAGNRKVRWDRIIPDSDGAGVIDAVGAGVDSARIGERVWIYNGQWDRPHGTSAQY
                     IALPAELAVPLSDALSYEEGACLGIPVMTAHRCLFADGPIAGKTVLVTGGAGVVGHYA
                     IQLAKWAGARVVTTVSSAAKAAHARAAGADVVINYRADPVVERVLDTVGGVDHIVEVD
                     FGANLPITARILREHGVVASYASARELNPPFPYVEFFRLNPVIRPVFVYTMPDTAKAE
                     AHTDIARWLTTSKPIFAIAARYALSDVVQAHLEVERGQKIGHVILKVD"
     gene            complement(2044..3159)
                     /gene="dnaJ"
                     /locus_tag="VFY80_01775"
     CDS             complement(2044..3159)
                     /gene="dnaJ"
                     /locus_tag="VFY80_01775"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6006659.1"
                     /translation="MAKKDYYEVLGVNRDASEDDIKKAYRKLAMKYHPDRNPDNPKAE
                     ERFKEAKEAYEILTDANRRAAYDQYGHAGVDPSAAAGAGAGFGGFADAFGDIFGDIFG
                     GGRTRSGVYRGADLRYNLEISLEEAARGTETRIRIPAMEECETCHGTGAKPGTNPSSC
                     PTCHGHGQVRMQQGFFSIQQTCPRCHGTGKIISHPCASCSGTGRIKKHKTLSVKIPAG
                     VDEGDRIRLSAEGEAGVSGGPPGDLYVVIHIKAHPVFQRDHNDLHCEMPISITTAALG
                     GEIEIPTLDGYAKIRIPQETQSGKVFRLRGKGIKGVRSTTHGDLLCHVVVETPVNLTP
                     RQKELLQELESINHKDAGRHNPRAKSWMDKVREFFES"
     gene            complement(3304..>4700)
                     /gene="dnaK"
                     /locus_tag="VFY80_01780"
     CDS             complement(3304..>4700)
                     /gene="dnaK"
                     /locus_tag="VFY80_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6006660.1"
                     /translation="KKKGDRKIAVYDLGGGTFDISIIEIAEVEGEHQFEVLSTNGDTF
                     LGGEDFDQRLMDYLCDEFKKESGIDLRKDMLALQRLKDAAEKAKIELSSSQQTEVNLP
                     YITADQSGPKHLAIKITRAKFESLVEDLIERTIEPCRIAIKDAGVKISEIEDVILVGG
                     QTRMPKVQEKVKEFFGKEPRKDVNPDEAVAVGAAIQAGVLKGDVKDVLLLDVTPLSLG
                     IETLGGVMTKLIQKNTTIPTKAQQVFSTADDNQTAVTIHVLQGEREMARDNKSLGQFN
                     LTDIPAAPRGMPQIEVTFDIDANGILHVSAKDKATGKENKIKIQASSGLSEAEIQRMV
                     KDAEAHAEEDKKARELVDARNQCDHMVHSVRKSLKEYGDKVPADEKAKIEAALKDAEQ
                     VLKGDDKDEIEAKTQALAQAAHKLAEQMYKEQQAGAQAQGQAGAEAKAGEARKEDDGN
                     VVDAEYEEVKDKKG"
BASE COUNT          756 a         1463 c         1491 g          990 t
ORIGIN      
        1 gggacccgcc atgctggtga actgcgttgt ctaccacgag gggagaaagc ttgcggacat
       61 ccgcaaggag gacatccacg tctacctcga ccggccgggc tgtttcgtgt gggttgcgct
      121 gaaggatgcg acgctggcgg aactcctgga gatgcagcag caattcgggc tgcacgagct
      181 tgcggtggag gatgcgagcc acggccacca gcgtcccaag ctcgaggaat atggcgacgc
      241 gctcttcgcg gtggtgcaca ctgtggagga gcacaacggc gaactgcagg tgggcgaagt
      301 gagcgtcttc gtcggcagga actacgtgct ttccatccgc aatcgcgcgc atgaggggtt
      361 tgccgacgtg cgcgcccggt gcgagcgcga gccgcagcac ctggcgctcg gcgccggatt
      421 cgtgctgtat gcgctgatcg atacggtcgt ggaccgctat ttcccggtgc tggaatatct
      481 agaagacgag ctggacggtg tcgagcggcg aattttttcc ggtacgccgg cgcggcagaa
      541 catcgaagca ctgtatcagc tcaagcagaa aatcatgacg ctgaggcacg ccgccgcgcc
      601 cctgcaggag accgcgtcca agctgcacgg tggacgcgtt cccgaggtct gcatgggcac
      661 gcaggactac tttcgcgacg tttacgacca cctcgtccgg atcaaccagt cgatcgagaa
      721 cttgcgggag atggtgacaa cggcattgtc ggtcaacctc tcgctcatca cgctgggcga
      781 gaacgaaacg acgaaacgcc tcgctgcgta cgccgcgctc gtcgccgtgc cgacgatgat
      841 cgcgggcatc tacggcatga acttcgatta catgcccgag ctgcggtggg cgttcggcta
      901 tccgctcgcg gtaggcgcga tggccgcgat tgacgggtat ttgttctatc ggtttcgaag
      961 ggcgaagtgg ctgtagcgga tggactgact ggcgaggacg atatgcgcgc ggcatggtat
     1021 caaagaaacg gagcggcagc ggaagtgctc gtggtcggcg atctgcccga tcctctcccg
     1081 gccgccggtg aagtgcgggt gcggctctat gcctcggggg tcaatccgtc cgacgtcaag
     1141 gcgcgtgcgg gcaaccgcaa ggtgcgctgg gatcgaatca tcccggatag cgacggggca
     1201 ggtgtcatcg atgcggtcgg ggcgggcgtg gacagcgcgc gcatcggcga gcgcgtatgg
     1261 atctacaacg ggcagtggga tcgtccgcac gggaccagcg cgcaatacat cgcgcttccc
     1321 gccgagctcg ccgtaccgct ctctgatgcg ctgagctacg aagagggcgc ctgtctgggc
     1381 attcccgtca tgacggcgca ccgctgcctt tttgccgacg ggccgatcgc cggcaagacc
     1441 gtgctggtga cgggcggggc gggagtcgtg gggcactacg ccatccagct cgccaagtgg
     1501 gccggcgcgc gcgtcgtgac gacggtgagc agcgcggcga aggccgcaca tgcgcgcgca
     1561 gcgggcgcag acgtggtcat caactaccgt gccgatccgg tcgtcgaacg cgtgctcgac
     1621 acggtaggag gtgtcgatca catcgtcgaa gtggatttcg gcgcgaatct gccgatcacg
     1681 gcgcggatac tgcgcgagca tggtgtcgtc gcgagctacg catccgcccg cgaactgaat
     1741 ccgccctttc cctacgttga gtttttcagg ctcaatcctg tcatccggcc ggtattcgtc
     1801 tataccatgc ccgacacggc gaaggccgag gcgcatacgg atatcgcgcg ctggctgacc
     1861 acctcaaagc cgatattcgc gatcgctgcg cgctatgcgc tttccgatgt cgtgcaagca
     1921 catctggaag tggagcgcgg ccagaagatc gggcacgtca tcctgaaggt cgattgatcg
     1981 gatagccgaa acccgcgggt ctttggcgct ggcttcgaaa aatccgcccg ggacgcgcgg
     2041 cagctatgac tcgaagaatt cccgcacctt gtccatccag gatttggcgc gcggattgtg
     2101 gcgaccggcg tccttgtggt tgatcgactc cagttcctgc agcagctcct tctgtcgcgg
     2161 cgtgagattg accggtgttt cgaccacgac gtggcagagg agatcgccgt gggtggtgga
     2221 gcggacaccc ttgatgccct tgccgcgcag ccggaatacc ttgccggact gcgtttcctg
     2281 cgggatgcgg atcttggcgt aaccgtcgag cgtcggaatt tcgatctcgc cgcccagcgc
     2341 cgcggtggta atgctgatcg gcatctcgca gtgcagatcg ttgtgatcgc gctgaaacac
     2401 cgggtgcgcc ttgatatgga tcacgacgta gagatcgccg ggtggcccgc cgctcacccc
     2461 ggcctcgcct tcggctgaca gccgaatgcg gtcgccttcg tccacgccgg cggggatctt
     2521 gaccgacaac gtcttgtgct tcttgatgcg gcccgtgccg ctgcagctcg cgcacggatg
     2581 gctgatgatc ttgccggtcc cgtgacagcg cgggcaggtc tgctggatcg agaagaagcc
     2641 ctgctgcatg cgtacctggc catggccatg gcaggtcggg caagagctgg gattcgtgcc
     2701 gggcttggcg ccggtgccgt ggcacgtctc gcattcttcc atggccggta tgcggatgcg
     2761 cgtctcggtg cctcgtgcag cttcctcgag cgagatttcg aggttgtagc gtagatcggc
     2821 gccgcggtag acgcccgaac gagtccgccc gccgccgaaa atgtcgccga aaatatcgcc
     2881 gaaggcatcg gcgaatccgc caaagcctgc gcccgcacct gcggcagcgg agggatcgac
     2941 gcccgcgtgt ccgtactgat cgtatgccgc gcgcctgttg gcgtcggtca ggatctcgta
     3001 cgcttccttc gcttccttga agcgctcttc ggccttgggg ttgtccggat tgcggtccgg
     3061 atggtacttc atcgcgagct tgcgatacgc cttcttgatg tcgtcttcgg atgcgtcccg
     3121 attgaccccg agcacttcgt agtaatcttt tttcgccatc tcttattggt gcactcaaat
     3181 agtcgacggc cgaaccaagg gacggctttc agcccgccac ttcagcccgg ccagtctcgc
     3241 gtcccgtgtg cgcgccgcca cacggaactt accacacgtt tcactccgcg cgaacgaggc
     3301 agatcaaccc ttcttgtctt tcacttcctc gtactcggca tcgacgacgt tgccgtcgtc
     3361 ttccttcctg gcctcgcccg ccttcgcctc ggcaccggcc tggccctgcg cctgcgcacc
     3421 ggcctgctgc tccttgtaca tctgttccgc aagcttgtgc gcggcctggg cgagcgcctg
     3481 tgtcttcgct tcgatttcgt ccttgtcgtc gcctttcagc acctgctcgg cgtccttcag
     3541 cgcagcctcg atcttcgcct tttcgtccgc gggcactttg tcgccgtatt ccttcaacga
     3601 cttgcgcacg gaatggacca tgtggtcgca ctggttgcgc gcatccacga gctcccgggc
     3661 cttcttgtct tcttcggcat gcgcctctgc gtccttcacc atgcgctgga tttccgcctc
     3721 ggacaggccg ctcgaggcct ggatcttgat cttgttctcc ttgcccgtcg ctttgtcctt
     3781 ggcggagacg tgcaggatgc cgttcgcgtc gatgtcgaaa gtgacctcga tctgcggcat
     3841 gccgcgcggc gcggccggga tgtcggtcag gttgaactgg ccgagcgact tgttgtcgcg
     3901 cgccatttcg cgctcgccct gcagcacgtg gatggtgacc gcagtctggt tgtcgtccgc
     3961 ggtcgagaac acctgctgtg ctttggtcgg gatggtggtg ttcttctgga tcaacttcgt
     4021 catcacgccg ccgagcgttt cgatgccgag cgaaaggggc gtcacgtcga ggagcagcac
     4081 atctttcacg tcacccttca gcacgccggc ctggatcgca gcgcccacgg caaccgcctc
     4141 atcagggttc acgtccttgc gcggctcctt gccgaagaac tccttcactt tttcctggac
     4201 cttcggcatg cgcgtctggc cgccgacgag gatgacgtcc tcgatctcgg aaatcttcac
     4261 gcccgcatct ttgatcgcga tgcggcacgg ctcgatggtc ctctcgatga ggtcttccac
     4321 cagactttcg aacttggcgc gcgtgatctt gatcgcgagg tgtttcggac cggactgatc
     4381 ggccgtgatg tacggcaggt tgacctcggt ttgctgcgag gatgaaagct cgatcttcgc
     4441 cttctccgcg gcgtccttca gccgttgcag ggcgagcatg tccttgcgca gatcgatgcc
     4501 cgattccttc ttgaactcgt cgcagaggta atccatcagg cgctggtcga agtcctcgcc
     4561 gccgaggaac gtatcgccgt tggtcgagag tacttcaaac tggtgctcgc cctcgacttc
     4621 cgcaatctcg atgatcgaaa tatcgaacgt gccgccgccc agatcgtaga cggcaatctt
     4681 gcgatcgccc ttcttcttgt
//
LOCUS       DASURG010000107         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_122590, whole genome shotgun sequence.
ACCESSION   DASURG010000107 DASURG010000000
VERSION     DASURG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122590"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFY80_01785"
     CDS             complement(<1..178)
                     /locus_tag="VFY80_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HEX6006661.1"
                     /translation="MIHILVHDRKDTVGVAVVEGIKAGQELIGWVMEDDSTIKVKALN
                     DIPIGHKVATRAIGK"
     gene            327..1082
                     /locus_tag="VFY80_01790"
     CDS             327..1082
                     /locus_tag="VFY80_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6006662.1"
                     /translation="MIGTLAAGALAPNPLYKEVKSRITRELAAGVWKPGAPIPSETRL
                     AREFSVSIGTIRKAIDELVAERILLRQQGRGTFVATHSEDRTLFYFFHIVGKDGSREP
                     PVTEMLSFREARAGKEHAARLGTARGAPTLRIQNILKLRGKPVVFDEIVVPGALFPDL
                     DQRTFGGRVGTIYGLYQARYSINVIRISERLSAALATKTVAARLNVRAGAPVLVIRRV
                     AYSYNDTPVEYRVSSVNTEAHDYLSDLWKTEGR"
     gene            complement(1111..1509)
                     /gene="rplS"
                     /locus_tag="VFY80_01795"
     CDS             complement(1111..1509)
                     /gene="rplS"
                     /locus_tag="VFY80_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX6006663.1"
                     /translation="MDLIKTLEQEEIKRLGKSIPDFSTGDTVVVNVNVVEGDRKRVQA
                     YEGVVIGKRNRGLNSSFIVRKISSGEGVERTFQTYSPLIASIEVKRRGDVGRSKLYYL
                     RQRSGKAARIKEKLTGGAPGAEAETQEQPS"
     gene            complement(1512..2270)
                     /gene="trmD"
                     /locus_tag="VFY80_01800"
     CDS             complement(1512..2270)
                     /gene="trmD"
                     /locus_tag="VFY80_01800"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX6006664.1"
                     /translation="MLQFDVVTLFPRMFEAVTERGVTGRARDRGLYHLVAWNPRDFAA
                     NAYRTVDDRPYGGGPGMVMMPEPTVRALDAARERQKSTGVGKPRVVYLSPQGRLLDHA
                     AVGELASETGLVLVAGRYEGVDERVIERRVDDEISIGDYVLSGGELAAMVLIDCVVRQ
                     LPGVLGDAESASQDSFANGLLDCPHYTRPEVFEGVAVPQVLLSGNHAEIARWRLKQAL
                     GRTWRRRPDLIARRALSEEERALLNEYRKEAEGA"
     gene            complement(2270..>2693)
                     /gene="rimM"
                     /locus_tag="VFY80_01805"
     CDS             complement(2270..>2693)
                     /gene="rimM"
                     /locus_tag="VFY80_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX6006665.1"
                     /translation="TESLEGLLAYRAWWLGGGTRWRRYQVTEARVRGEAVVAKLAGCE
                     DRDAAAALRGQEVAVSREELPHPAANEWYWSDLIGLRVINLAGEDLGVIARMLRTGAN
                     DVLVVEAERERLVPFIAGVVKEVDLAARVMRVDWSAES"
BASE COUNT          470 a          947 c          801 g          475 t
ORIGIN      
        1 ccttcccgat cgcccgggtg gccactttgt ggccgatggg aatgtcgttc agcgccttca
       61 ccttgatcgt ggagtcgtcc tccatcaccc agccgatcag ctcctgaccg gccttaatgc
      121 cttcgacgac ggcgaccccg acggtgtcct tcctgtcgtg tacgagtatg tggatcatgt
      181 gctctcctct tgctggtctg acgcgttcac gagcggcgcg cgaaccccgc actttacgcg
      241 ccgcgcggag ggttagtcaa ctatatgagt tagatgaatt tggcgttggc gtgctacact
      301 gcggcgccat tgacccgggc cacacgatga tcggtacgct cgccgcaggc gctctcgcgc
      361 cgaacccgct gtacaaggaa gtaaagtcgc gtatcacgcg cgaactcgcg gcgggggtgt
      421 ggaagccggg cgcgccgatt ccgagcgaga cccggcttgc gcgcgaattc agcgtatcca
      481 tcggcaccat acggaaagcg atcgacgagc tcgttgcgga acgcatcctc ctgcgccagc
      541 aagggcgtgg tacgttcgtt gctacccaca gcgaagaccg cacgctgttc tatttttttc
      601 atatcgtggg caaggacggc agccgcgagc cgccggtgac ggagatgctg tcatttcgcg
      661 aggcgagggc cggcaaggag cacgcggccc ggctcgggac cgcacgcggc gccccgaccc
      721 tgcgcatcca gaacatcctc aaactcaggg gcaagccggt ggtcttcgac gagatcgtgg
      781 tgccgggcgc actcttccca gatctcgacc agcggacctt cggcgggcgc gtcggcacga
      841 tttacgggct ctaccaggcg cgctacagca tcaacgtgat acgcatcagc gaacggctgt
      901 cggcggcgct cgcgacgaag acggttgccg cgcggctcaa tgtccgcgca ggcgcgccgg
      961 tgctggtgat caggcgtgtc gcttatagct acaacgacac gccggtcgaa taccgggtga
     1021 gctcggtgaa caccgaagcc catgactacc tgagcgatct gtggaaaacc gaaggccgat
     1081 gagcttgggc tcgcccgcct tcggtgcagc tcaggacggc tgttcctgcg tctcggcttc
     1141 cgcgccgggt gcgccgccgg tcaacttctc cttgatgcgt gcagccttgc cggaacgctg
     1201 gcgcagataa tagagcttcg agcggccgac atccccgcgg cgcttcacct cgatcgacgc
     1261 gatgagcggc gaataggtct ggaaggtgcg ctccacgccc tcgccggacg agatcttgcg
     1321 cacgatgaac gaggagttga gcccgcgatt gcgcttgccg atcaccacgc cctcgtacgc
     1381 ctgcacccgc ttgcggtcgc cttcgacgac gttcacgttc acgaccacgg tatcgccggt
     1441 cgaaaagtcg ggaatgctct tgccgagccg cttgatttct tcctgctcga gcgtcttgat
     1501 gagatccatg ttcatgctcc ttcagcttct ttccggtact cgttgagcag cgcacgctcc
     1561 tcttcgctga gcgcccgacg cgcgatcaga tccggacgcc gccgccaggt gcgccccaag
     1621 gcctgcttga gtcgccagcg cgcgatctcc gcatggttgc cggagaggag cacctgcggc
     1681 accgccacgc cttcgaacac ctcgggccgc gtatagtgcg ggcagtcgag tagtccgttc
     1741 gcaaacgaat cctggcttgc ggattccgca tctcccagca cgccgggaag ctggcggacc
     1801 acgcagtcga tcagcaccat cgcggcgagt tccccgccgg aaagcacgta atcgccgatc
     1861 gagatttcat cgtccacgcg gcgctcgatc acgcgctcgt ccacgccctc gtagcgtccg
     1921 gcgacgagca ccagtcccgt ctcggatgca agttcaccaa ctgcggcgtg atccaaaagc
     1981 cgcccctgcg gcgacagata gaccacgcgc ggcttcccta cgcccgtgct cttctgccgc
     2041 tccctcgccg cgtcgagcgc gcggaccgtc ggctccggca tcataaccat gcccggtccg
     2101 ccgccgtacg ggcggtcgtc caccgtccgg tacgcgttcg ccgcaaaatc gcgcgggttc
     2161 cacgcgacaa ggtggtaaag cccgcgatcg cgcgcccggc cggtcacacc ccgttcggtc
     2221 accgcctcga acatgcgcgg aaacagcgtg acgacatcga actgaagcat caggactccg
     2281 cgctccaatc cacgcgcatc accctggcgg caaggtccac ttccttcacc accccggcga
     2341 tgaacggaac gagccgctca cgctcggctt ccactaccag cacatcattc gcaccggttc
     2401 gcagcatccg ggcgatcact cccagatctt caccagcgag gttgatgacg cgcagcccga
     2461 tcaggtccga ccagtaccac tcgttcgcag caggatgagg cagctcctcg cgcgacaccg
     2521 ccacttcttg cccgcgcaac gccgcggccg catcgcgatc ctcgcaaccc gcgagcttgg
     2581 caaccacggc ttcgccccgc acgcgtgctt ccgtaacctg gtaccgcctc caccgcgtac
     2641 cgcccccgag ccaccaagcg cggtaggcga gtaggccctc aagcgattcc gta
//
LOCUS       DASURG010000108         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_327846, whole genome shotgun sequence.
ACCESSION   DASURG010000108 DASURG010000000
VERSION     DASURG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327846"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..383)
                     /locus_tag="VFY80_01810"
     CDS             complement(42..383)
                     /locus_tag="VFY80_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006666.1"
                     /translation="MNSAEALVMILFALGIATVHAKLPAPTPEEQAAVEKKKAAQQAQ
                     LEKEKKQLEAAQDRVVQHYRRSKGGTADGGQGAQTQAENMPKTAKELPGGVGPKPTQP
                     QSGEAHSAPAK"
     gene            503..1186
                     /locus_tag="VFY80_01815"
     CDS             503..1186
                     /locus_tag="VFY80_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6006667.1"
                     /translation="MSTKGTFRSAVFAAALCSASAPALAAPSHAASEAMWEEALAGFA
                     AADLRHAPEPGGVLFVGSSSIRLWDNLESDFADARVIKRGFGGSRLSDCVRLMDRLVI
                     RYRPRIVMLYAGDNDLAEGSTPDEVLARVKAFAEGVHSRLPETRVRFISIKPSPARQR
                     LIDQARAANRLVREYAESHPALDYIDVFTPMLTAEGRPRRELFRADALHLNDSGYALW
                     RAIIRPFVR"
     gene            complement(1164..2300)
                     /gene="opgC"
                     /locus_tag="VFY80_01820"
     CDS             complement(1164..2300)
                     /gene="opgC"
                     /locus_tag="VFY80_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX6006668.1"
                     /translation="MTRRPELDALRGLMLILMAITHVPTRYSTWLSQPFGFVSAAEGF
                     VFLSAYMVGAIYTRRAMESGTGAMRAALWRRAGLVWLCHVGMLLFLFTLIAKIGLRTD
                     RRAIKNLISFYLEEPIDALWTGLIMIYNPPLLDILPMYVVYMVYSPIALTLGLRRSGW
                     YVVIAGSLTIWASAQLYLIQPLYKAFIHWIEPKMPFHQTGAFDLFAWQFIWVLGLWLG
                     SRREAIPERRAIPGWIIAFSLTFALTCLVWRHAVGQAPFGSVAELNVLFDKWRLGPLR
                     IVNFFALLVLVIRYGPYLLKFRFPVLETLGRASLPVFCAHVVIVLVVLAMIGDRVGRT
                     PLWAESALLVATLVALYAVAVFSNRLAREPRERARPSISARKAE"
     gene            2396..>3022
                     /locus_tag="VFY80_01825"
     CDS             2396..>3022
                     /locus_tag="VFY80_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6006669.1"
                     /translation="MDIRIPLRIAAIGVGHWHALHDAAYLRTLVRMPDVKLVAVHDGD
                     EALAAQRARELGHPQIYTDYRAMLAACRPDFVLALGRHSEMAQVAHTLLDEGLPFLME
                     KPMGLNAAEVRGIADKARAKCAYAATPLFQRYQPFVLQAQRMLAEGAFGPVSHLYFRS
                     NRGSSARYVAWGAPWMLDPAMAGGGCLRNIGLHGIDAFIRLFGEPEVTG"
BASE COUNT          542 a          994 c          965 g          521 t
ORIGIN      
        1 gtgagacgga gtgggtgcaa ctgcgcggct cgcctcagac gctacttggc cggcgccgag
       61 tgcgcttcgc cgctctgcgg ctgcgtgggc ttcgggccta caccgcccgg cagctccttg
      121 gcagtcttcg gcatgttctc cgcctgcgtt tgagcgccct gcccgccgtc agcagtcccg
      181 cctttgctcc tgcggtaatg ctggacgacc cgatcctgcg ccgcttcgag ctgtttcttc
      241 tccttttcca gttgggcctg ttgcgccgcc ttttttttct ccaccgccgc ctgctcttcc
      301 ggcgtaggcg ccggtagctt ggcatgtacg gtggcgatac cgagtgcgaa caggatcatg
      361 accagggctt cagctgagtt cacgggacct cctcgatgca atgtggacaa atccttagag
      421 cttggcggac cggggaaagt tgtagtaagt tcggcgtaag gaaccctgca ccgttatctg
      481 tgttcaatcc ggtatggccg tcatgagcac caagggaact tttcgcagcg cagtatttgc
      541 cgcggcgctc tgctcagcaa gcgccccggc gctggcggcg ccatcgcacg cggcgagcga
      601 ggcgatgtgg gaagaggcgc tcgccggctt tgcagcggcc gacctccggc acgcgcctga
      661 accgggtggc gtgctgttcg tcggcagttc ctcgatccgc ctgtgggata acctcgagtc
      721 ggatttcgcc gacgcgcggg tgatcaagcg cggcttcggg ggctcgcgcc tttccgactg
      781 cgtgcgcctc atggaccggc tggtcatccg ctaccgaccg cgcatcgtca tgctctatgc
      841 cggcgacaac gaccttgccg aaggcagcac gcccgacgag gtgctcgcgc gcgtgaaggc
      901 gttcgccgag ggcgttcaca gccgtctgcc agagacacgc gtccgcttca tctccatcaa
      961 gccgagcccc gcgcgccaga ggctgatcga tcaagcgcgc gccgccaacc gtctggtgcg
     1021 cgagtacgcc gagagtcacc cggcactcga ctacatcgat gtgttcacgc cgatgcttac
     1081 cgcggaaggc cggccgcgcc gcgaactttt cagggcggac gcgctgcacc ttaacgactc
     1141 cggctacgcg ctctggcgcg ccatcattcg gcctttcgtg cgctgatcga cggtcgggcg
     1201 cgctcacgcg gctcgcgcgc aagccggttg ctgaacactg ccaccgcgta gagcgcgacg
     1261 agcgtcgcga cgagcagcgc actttcggcc cagagcggtg tgcgccccac gcgatcgccg
     1321 atcatcgcca gcacaaccag cacgatgacg acgtgcgcgc agaacactgg cagcgaggcg
     1381 cggccgagcg tttccagcac cggaaagcgg aacttcagca ggtacgggcc gtagcggatc
     1441 acgagcacga gcagcgcaaa gaaattcacg atccgcagcg ggccgagccg ccacttgtcg
     1501 aaaagcacgt tcagttcggc gaccgagcca aagggcgcct gtcccaccgc atgccgccac
     1561 accaggcacg tcagcgcaaa cgtcagtgaa aaagcgatga tccagccggg gatcgcgcga
     1621 cgttccggaa tcgcctcgcg gcgcgagccc agccacaggc cgagcaccca gatgaactgc
     1681 caggcgaaga gatcgaacgc accggtctgg tggaacggca tcttgggctc gatccagtgt
     1741 atgaacgcct tgtagagcgg ctggatcagg tagagctgag ccgacgccca tatcgtgagg
     1801 ctgcccgcga tcacgacata ccagccgctg cgacgcagcc cgagcgtgag cgcaatcggc
     1861 gagtacacca tgtacaccac gtacatcggg aggatgtcga gcagcggcgg gttgtagatc
     1921 atgatgaggc cggtccacag cgcatcgatc ggctcttcga ggtagaacga gatcaaattt
     1981 ttgatcgcgc gtcggtcggt gcgcagcccg atcttcgcga tcagcgtaaa cagaaagagc
     2041 agcatgccga cgtggcacaa ccagacgaga cccgcgcggc gccacagggc ggcccgcatc
     2101 gcaccggtgc cgctctccat cgcgcgtcgc gtgtagatcg cacccaccat atacgccgaa
     2161 aggaagacga aaccctccgc tgccgaaacg aatccgaagg gctggctgag ccacgtgctg
     2221 tagcgcgtcg gcacgtgcgt gatggccatg aggatcagca tcaatccccg cagcgcatcg
     2281 agttccgggc gtcttgtcat ggtcgagccg ctccagtcag tggaccgcgg gcagcgccaa
     2341 aggttgtgtc cggtatggta gcctcgcgta acccgaattc cgagcaggcc ccggcgtgga
     2401 catccgcatt ccgctgcgta tcgcagcgat cggcgtagga cattggcacg cgctccacga
     2461 cgccgcctac cttcggacgc tggtccggat gccggatgtg aaactcgtag ccgtgcacga
     2521 tggagacgaa gcactcgcgg cgcagcgggc gcgcgaattg ggccatccgc agatctatac
     2581 cgactaccgg gcaatgctgg cggcgtgccg gccggacttc gtcctcgccc tcggccgtca
     2641 cagcgaaatg gcgcaggtcg cacacacgct gctcgacgaa ggtctaccgt ttctcatgga
     2701 aaagccgatg ggcctcaatg cggcggaagt gcgcggcatc gcggacaagg cccgtgcaaa
     2761 gtgcgcgtac gccgcgacac cgctcttcca gcgctatcag cctttcgtgc tccaggcgca
     2821 acgcatgctg gcggagggcg cattcggtcc cgtatcgcat ctctattttc gctccaatcg
     2881 cggaagttcg gcacgctatg tggcgtgggg cgcgccgtgg atgctcgatc ctgcgatggc
     2941 tggcggtggc tgcctgcgca acatcgggct ccacggcatc gatgcgttca ttcgcctctt
     3001 cggcgaaccg gaagtgaccg gc
//
LOCUS       DASURG010000109         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_21565, whole genome shotgun sequence.
ACCESSION   DASURG010000109 DASURG010000000
VERSION     DASURG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21565"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFY80_01830"
     CDS             <1..369
                     /locus_tag="VFY80_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6006670.1"
                     /translation="RELHGAGFLLGVATGKSRRGLNRALEATGLASYFHATRCADEGH
                     CKPHPGMLTELMATVGVPANRTLMIGDTSHDMAMAQSAAVARVGVGYGAHVKQALLPY
                     APLACVDAVTELRAWLMTNA"
     gene            437..1396
                     /locus_tag="VFY80_01835"
     CDS             437..1396
                     /locus_tag="VFY80_01835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX6006671.1"
                     /translation="MADPEPHKSEEHWERAVLEKVALAAVQEQRRARHWGIFFKLLLF
                     IYLFALLFIAMGWWGKKDSLPGKHTALVEIRGVIGAEGSASADNVTTGLQDAFKDKRT
                     QGVIVRINSPGGSPVQAGHINDEIRRLRDKYPNIPLYAVVEDVCASGGYYVAVAADRI
                     FVDKSSIIGSIGVLMDGFGFTGAMEKLGIERRLLAAGENKGFLDPFSPIEESQKAHAQ
                     NMLSEIHQQFINVVREGRGDRLKETPEMFSGLLWIGTKSIELGLADAMGSVEYVAREV
                     IKAEDIVDFTPRENIAERVVRRFGAAMAETLLRITTSNGLAVR"
     gene            complement(1416..>2025)
                     /locus_tag="VFY80_01840"
     CDS             complement(1416..>2025)
                     /locus_tag="VFY80_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6006672.1"
                     /translation="VENPKSARQFLKAVGYPYPLQSVRMHVLDEHTPETVLPELLAPV
                     LAGQDCGLLSEAGCPAVADPGALLVRLAHENGIRVVPLVGPSAVLLALMASGLNGQRF
                     AFHGYLPVQAAERRRKLQDLERESQRGDCTQIFIEAPYRNNSLLRAILDACRDDTLLC
                     LATDISVQSEAIKTRPVKAWRNQVPELDRRPTVFLLYSQTGR"
BASE COUNT          400 a          601 c          676 g          348 t
ORIGIN      
        1 agggagctgc acggggccgg ctttctgctg ggggtcgcga ccggcaagag ccggcgcgga
       61 ttgaaccgtg cgctggaggc aaccgggctt gcctcctact tccacgcgac gcgctgtgcc
      121 gacgaggggc actgcaaacc gcatccgggc atgctgacgg aactcatggc tacggtcggc
      181 gtgcctgcga accggacgtt gatgataggc gataccagtc acgacatggc catggcgcag
      241 agcgccgcgg tcgctcgggt cggcgtcggg tatggcgccc atgtaaagca ggcgcttttg
      301 ccctacgcgc cgctcgcctg cgtggacgcg gtcaccgaat tgagagcatg gctgatgacg
      361 aacgcatgag tgccaaccgg ctataggcgc aggctatact ccttcctcac aatttgacgg
      421 tatgcagggt gcagatatgg ctgatccgga accccacaaa tcagaagagc actgggagcg
      481 cgctgtcctc gaaaaagtgg cgctcgcggc cgtacaggag cagcggcgcg cgcggcactg
      541 gggaattttc ttcaagctgc tgctcttcat atacctcttt gcattgctct tcatcgccat
      601 gggatggtgg ggcaagaaag actcgctgcc gggcaagcac accgcgctcg tcgagatccg
      661 cggcgtcatc ggcgcggaag gcagcgcgag cgcggataac gtcacgacgg gcttgcagga
      721 tgcgttcaag gacaagcgca cccagggcgt gatcgtccgt atcaacagcc cgggcggcag
      781 cccggtgcag gccgggcaca tcaacgatga gatacgccgg ctgcgggaca agtatcccaa
      841 cattccgctg tacgccgtcg tggaggacgt ctgtgcctcg ggcggatact acgtggccgt
      901 agcggctgac cgcatattcg tggacaagtc gagcatcatc ggttccatcg gcgtgctcat
      961 ggacggcttc gggttcaccg gcgcgatgga aaagctcggt atcgagcggc gcctcctcgc
     1021 tgcgggtgaa aacaagggct tcctcgatcc attctcgccg atcgaggaat cgcagaaggc
     1081 gcatgctcag aacatgctga gcgagattca ccagcagttc atcaacgtcg tgcgcgaggg
     1141 gcgcggagac cgcctcaaag agacgccgga aatgttctca gggctgctgt ggatcgggac
     1201 caagagcatc gagctcgggc tcgccgatgc aatggggagc gtcgagtacg tggcgcgtga
     1261 ggtcatcaag gcggaggaca tcgtcgactt tacgccgaga gagaacattg cggagcgggt
     1321 agtgcggcgg tttggggcag ccatggccga gacgctcctt cgcatcacca cttcgaacgg
     1381 cctcgccgtg cgctgaaagc ggggttgcga accggtcatc gccctgtttg tgaataaagc
     1441 agaaataccg tcggtcgacg atcgagctcg ggcacctggt tacgccaggc tttcaccggc
     1501 cgggtcttga tcgcctccga ttgcacggaa atatccgttg cgaggcagag aagcgtgtca
     1561 tcgcggcacg cgtccagaat cgcgcgcaac agactgttgt tgcgataggg cgcctcgata
     1621 aagatctgcg tgcagtcccc ccgctgcgac tcgcgctcga gatcctgcag cttgcggcgg
     1681 cgttccgccg cctgcaccgg cagatacccg tgaaaagcga aacgctggcc gttcagcccg
     1741 gaggccatca aagcaagcaa taccgcggac gggccgacga gcggcaccac gcgtattccg
     1801 ttttcatgcg ccaggcgaac gaggagcgca cccggatccg ctacggcagg gcaaccggct
     1861 tcggaaagca atccgcagtc ttgcccggcg agcacggggg cgagcaactc aggcagcact
     1921 gtctccggcg tgtgctcgtc gaggacgtgc atgcggacac tctggagtgg atagggatag
     1981 ccgaccgcct tgaggaactg gcgcgcgctc ttgggattct cgacg
//
LOCUS       DASURG010000110         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_837793, whole genome shotgun sequence.
ACCESSION   DASURG010000110 DASURG010000000
VERSION     DASURG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837793"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFY80_01845"
     CDS             complement(<1..969)
                     /locus_tag="VFY80_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6006673.1"
                     /translation="MREGLASRLADIEPFRVMEILARARELERAGRSIVHMEIGEPDF
                     PTPRPICEAGIAALEKGELHYTPAQGLPALREAIAQYYQRHYGIDVSASRIIVTTGSS
                     GALLLAVSALVDAGDRVLLADPGYPCNRHFVRLVEGDPVALAVGPDSNYQLTPELLDR
                     HWDGRTVAALVASPSNPTGTVVPPEALKAMAEVARARGGTLIVDEIYHGLVYEGGVTT
                     AAALSDEVFIINSFSKYFHMTGWRLGWMIVPERYVAEVDKLAQNIFLAAPTPAQYAAL
                     AAFKPETLAILEARRAEFKARRDYLVPALRALGFDIPQSPEGAFYIY"
     gene            complement(980..1933)
                     /locus_tag="VFY80_01850"
     CDS             complement(980..1933)
                     /locus_tag="VFY80_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6006674.1"
                     /translation="MKRWLVTAAAVLAFSAAAHAQQRLTISTGGTGGVWYPMGGAMAN
                     VLSKYLPNTSATAEVTGASVDNLKLIAGRKTDVGFSMVDTAWDAMNGTGKFKDSKVPM
                     RTLAVVHPLVMHVVTAEGRGIEKIADFKGKRISTGSPGSGTEVMALRILEAYGIDPNK
                     DVVRERLSVAEAANALKDRKIDAFMHAAGVPLPAVTDVAATPGIKAKLIDHADALEAM
                     RKKYGPIYAAGRIPANAYPGQERENRTINVWGVLFVHETMNEKLAYDIVKTLFDHHKE
                     LVTAHREAENMTLQNQTIGATAVPWHPGAVKYFTERGIKVK"
     gene            complement(2100..>2621)
                     /locus_tag="VFY80_01855"
     CDS             complement(2100..>2621)
                     /locus_tag="VFY80_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006675.1"
                     /translation="DKHCQSLAAAAGAGQRTWRAYLSTQAPALKDPNLVNARDRIGTG
                     PWQNAKGVVVARSVEELHGANNLTKETALDEKGRPVNGRTEKPNKHDILTGSRTDGTA
                     FPGAPFADMTCGNWTKGGKEGSAMTGHHDRGGLVNAPWAISWNSSHPTTGCDPASIRA
                     TGGDGLFYCFAAK"
BASE COUNT          436 a          825 c          838 g          522 t
ORIGIN      
        1 atagatgtag aacgcgccct ctggcgactg cgggatgtcg aaaccgagcg cgcgcagcgc
       61 cggcacgagg taatcgcggc gtgccttgaa ctcggcgcgg cgcgcttcga gtatggcaag
      121 cgtttccggc ttgaaggcgg caagcgcggc atactgcgcc ggtgtcggcg cggcgagaaa
      181 gatgttctgc gcgagcttgt ccacttccgc gacgtagcgc tcggggacga tcatccagcc
      241 caggcgccat ccggtcatgt ggaagtattt cgagaagctg ttgatgatga acacttcgtc
      301 gctcagcgcg gctgcggtag tgacgccgcc ctcgtacacg aggccgtgat agatctcatc
      361 gacgatcagc gtgccgcccc gcgcacgcgc aacctcggcc atggctttaa gcgcctccgg
      421 gggaacgacc gtgcccgtgg gattggacgg ggacgcaacg agcgccgcaa ccgtccgacc
      481 gtcccaatgc cggtcgagaa gttcgggcgt gagctgatag ttgctgtcgg gaccgactgc
      541 gagcgcaacc ggatcccctt cgacaagccg cacgaaatgt cgattgcagg gataaccggg
      601 atccgcgagc aacacgcggt cgcccgcatc aaccagtgcg gagacggcaa gcagaagcgc
      661 gccggaggaa cccgttgtca cgatgatgcg cgacgcggat acgtcgatcc cgtaatggcg
      721 ctgatagtac tgtgcgatcg cctcccgtaa tgccggcagg ccctgagccg gcgtgtagtg
      781 gagttcaccc ttttccagtg cggcgatgcc ggcttcgcag atcggccggg gggtgggaaa
      841 gtccggctcg ccgatttcca tgtgaacgat ggagcggcca gcgcgctcga gctcgcgcgc
      901 ccgcgcgagg atttccatca cgcgaaaggg ctcgatgtcc gcgaggcggg aggccagccc
      961 ttccctcact ccacgccttt catttcactt tgatgccgcg ctcggtgaaa tacttgaccg
     1021 cacccggatg ccagggcacc gcggttgcgc cgatggtctg gttctgaagc gtcatgtttt
     1081 ccgcctcgcg gtgcgcggtg acaagctcct tgtggtggtc gaacagcgtc ttcacgatgt
     1141 cgtaggcgag cttctcgttc atcgtctcgt gtacgaatag cacgccccat acgttgatcg
     1201 tgcggttttc gcgctcctgc ccgggatagg cgttcgccgg tatccgaccc gccgcgtaga
     1261 tcgggccata cttcttgcgc atcgcctcca gcgcgtcggc gtggtcgatt aacttcgcct
     1321 tgatgccggg agtagcggcc acatcggtga ccgcgggcag cggcacgccg gccgcgtgca
     1381 tgaaggcgtc gatcttgcga tccttgagcg cgttcgccgc ttccgcgacg ctcaagcgct
     1441 cgcgcaccac atccttgttg gggtcgatac cgtaggcctc gaggatgcgc agcgccatca
     1501 cttccgttcc gcttccggga gagccggtcg agatgcgctt gcccttgaag tcggcgatct
     1561 tttcaatgcc gcggccttcg gcggtcacga cgtgcatgac gagcgggtga accacggcaa
     1621 gcgtgcgcat cgggaccttg gagtccttga acttgccggt gccgttcatt gcgtcccatg
     1681 cggtgtccac catggaaaag ccgacgtcgg tcttgcggcc ggcgatgagc ttcaggttat
     1741 cgacgctcgc gccggtcacc tccgcggtcg ctgacgtgtt cggcaggtac ttggaaagca
     1801 cgttcgccat cgcgccaccc atcggatacc acacgccgcc ggtgccgccg gtcgatatcg
     1861 taaggcgctg ctgtgcatgt gccgctgcac tgaaggcgag tactgctgcc gctgtgacga
     1921 gccatcgttt catgtcatgc ctcccattcg tgagccgtaa tctctaccat tgccttcgcc
     1981 gtgcgcgtcc cgagaattga gacgcgtcat gatgcaccaa cttccagccg aaaaacaaat
     2041 ggcgtggccg ggtcggccac gccatcgtga aacgcgatga gaggagtgcg gtcaggctgt
     2101 catttcgcgg cgaaacagta gaagagcccg tcaccgcccg tagcccggat cgacgctggg
     2161 tcgcacccgg tcgttggatg agaggaattc cacgaaatgg cccaaggggc gttgaccagt
     2221 ccgccgcggt cgtggtgtcc ggtcatcgcc gaaccttctt tcccaccctt cgtccagttt
     2281 ccgcacgtca tgtcggcaaa cggcgcaccg gggaacgccg tcccatccgt ccgcgagccc
     2341 gtgaggatgt cgtgcttgtt tggtttttcg gtgcggccat tcacggggcg gcccttttcg
     2401 tccaacgccg tttccttggt gagattgttc gctccgtgca gttcctcgac gcttcttgcg
     2461 accacaacgc cttttgcatt ctgccagggg ccggtgccga tgcggtcgcg tgcgttgact
     2521 aagttcggat ctttcagcgc aggcgcctgt gtgctgagat aagcgcgcca cgtccgctgc
     2581 ccggcaccgg cggcggcagc aagggattgg caatgcttgt c
//
LOCUS       DASURG010000111         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_123581, whole genome shotgun sequence.
ACCESSION   DASURG010000111 DASURG010000000
VERSION     DASURG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123581"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..418
                     /locus_tag="VFY80_01860"
     CDS             32..418
                     /locus_tag="VFY80_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6006676.1"
                     /translation="MKHLEPKEAYEFLKKNPNTIFVDCRSEMEYLFVGHPVGAILVPW
                     NDGPNWDVNPEFVAHVKKAASVDRPIVLICRSGNRSLDAGRALEEAGFTKVYNVLHGF
                     EGELDENHHRNAKTGWRYDGLPWEQC"
     gene            complement(479..1513)
                     /locus_tag="VFY80_01865"
     CDS             complement(479..1513)
                     /locus_tag="VFY80_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6006677.1"
                     /translation="MQELDSWREEKRSAFLYRAVAEKEKGSTRAALFAELAEEADRQA
                     QIWADRIRTTGRDAPREFAPDARARLVAGLVRRMGVRPLRGVLAAMKVRGMSLYTHAR
                     PGHAMPQSVEEVGARHRGVEGGGNLRAAVFGVNDGLVSNASLILGVAGAIADNSIVLL
                     SGAAGLIAGAFSMAAGEYVSVRSQREMYEYQIALEREELDHYPEAEAEELALIYEARG
                     LNREEAASMAKTMTADPEVALKTLAREELGLNPDDLGSPWGAALFSFVSFAAGAAIPL
                     TPFFFVEGRASLLISIGLTGTALLGVGAALSLFTGRSALYSGLRMLAIGAAAGTVTYL
                     VGTLLGVTLG"
     gene            complement(1497..2693)
                     /locus_tag="VFY80_01870"
     CDS             complement(1497..2693)
                     /locus_tag="VFY80_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX6006678.1"
                     /translation="MPFLAAMLAGLAMIGPFTIDTYLPSFPAMGRDLAATPAQLQQTL
                     SVYFLTFAVMTLFHGTLSDSFGRRPVILASLLVYIAASLGCAAATDFAHLLFFRVLQG
                     TSAGAGMVVGRAVIRDSFEGHDAQRLMSLVTMIFGLAPAVAPVVGGWLQSAFGWQAVF
                     LFLAGYALVLLVVCWGYLPETHPATQRQPFEIGPLARNYLMLAQSRPLLLLCVGVGLN
                     FAGFFIYIVSAPAFVYDLLGLTERQFAWLFLPGIIGVMSGAFISGRVAGVLTPRRTIG
                     IAYAIMFCAGVSNILYSGFGPPAVPWSVLPIMIYTVGMALAMPSLTLLALEIFPRNRG
                     ATSSLQGFIHSFLSSVGAGVISPLVSQSDITLAFAMTGLMSLGFISWLLYLRLQPVAM
                     AHARTG"
     gene            complement(2896..>3190)
                     /locus_tag="VFY80_01875"
     CDS             complement(2896..>3190)
                     /locus_tag="VFY80_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006679.1"
                     /translation="ELFVAGAQQALNVMRHTSETMSLVQQQVGQLVEQQTAEVAEQLR
                     QGVEEVSRRTEETLDQLRQTTQQAAEEAQYAGGNGSSAEEAQSRRGRRGRRRG"
BASE COUNT          642 a         1055 c         1014 g          479 t
ORIGIN      
        1 tataatgacg tcccattcca gaggatccgc gatgaagcac ctcgagccga aagaggctta
       61 cgagtttctc aagaagaacc cgaacacgat cttcgtagac tgccgcagcg agatggaata
      121 cctcttcgtt gggcaccccg tcggcgcgat tctcgtcccg tggaacgacg ggccgaactg
      181 ggatgtgaat ccggagttcg tcgcacacgt gaagaaagcg gcgagcgtcg atcgtccgat
      241 cgtgctcatc tgccgcagcg gcaatcgctc gctcgacgcc ggccgcgcac tcgaggaagc
      301 gggattcacc aaagtctaca acgtgcttca cggcttcgaa ggcgagctcg acgagaatca
      361 ccaccgcaat gcgaagaccg gctggcggta cgacggcctg ccgtgggaac agtgctaaca
      421 aaaaacggga acgacggccc ccaaaaagaa acggtggccc gcgaagcgct aggcaacgct
      481 agccgagcgt cacgccgagc agcgtcccca cgagataagt gaccgtaccg gccgccgcgc
      541 cgatcgcgag catgcgcaag ccgctataga gcgcgctgcg accggtgaag aggctgagcg
      601 ccgcgccgac gccgaggagc gccgtgccgg tgaggccgat cgagatgagc aggctcgccc
      661 ggccctcgac gaagaaaaaa ggcgtcagcg gtatcgccgc gcccgccgca aacgaaacga
      721 atgaaaagag tgccgcgccc cagggcgacc cgaggtcatc gggattgagt cccaactcct
      781 cgcgcgcgag cgtcttcagc gccacctcgg gatccgccgt catcgttttc gccatactcg
      841 ccgcttcctc acggttgagc ccgcgcgctt catagatgag cgcgagctcc tcggcctcgg
      901 cctcgggata gtggtccagc tcctcgcgtt cgagcgcgat ctggtactcg tacatctcgc
      961 gctgcgagcg cacggataca tactcgccgg ccgccatgga aaacgcgccg gcgatgagcc
     1021 cggccgctcc ggagagcagc acgatgctgt tatctgcgat cgcgcccgcg acgccgagga
     1081 tcaggctcgc gttggagacg aggccgtcat tgacaccgaa taccgccgcc ctgagattgc
     1141 cgccgccttc caccccgcgg tgacgcgcac cgacttcctc caccgactgc ggcatagcgt
     1201 gcccggggcg ggcatgggtg tagagcgaca ttccgcgcac cttcatcgca gcgagcacgc
     1261 cccgcagcgg gcgcacaccc atgcgccgca ccagcccggc gacgagccgg gcgcgagcgt
     1321 ctggcgcaaa ttcacgcgga gcgtcgcgac ctgttgtgcg gatccgatcg gcccagattt
     1381 gtgcctggcg gtcggcttcc tcggccagct cggcaaagag cgccgcgcgg gtgctgcctt
     1441 tctctttctc ggcaacggcc cggtaaagaa atgcagagcg cttttcctcg cgccagctat
     1501 ccagttcttg catgggccat cgcaaccggc tgcaggcgca ggtagagcag ccatgaaatg
     1561 aacccaagcg acatcaggcc cgtcatggcg aatgcgagcg tgatatcgga ttgcgagacg
     1621 agcggcgaaa tcacgcccgc gcccacgctg gagagaaagc tgtggatgaa tccctgcagg
     1681 gacgatgtgg ccccccgatt gcgcggaaag atttcaagcg cgagcagtgt caggctcggc
     1741 atcgcaagcg ccatgccgac cgtatagatc atgatcggca acacggacca cggcacggca
     1801 ggcggtccga atccactgta caggatattc gacacgcccg cgcagaacat gattgcatac
     1861 gcgatgccga tcgtgcgccg gggcgtgagc acgcccgcga cgcgccctga aatgaacgcg
     1921 cccgacatga cgccgatgat gcccggcaga aagagccacg cgaactgtct ttcggtcaag
     1981 cccagcagat cgtaaacaaa agccggcgca gacacgatgt agatgaaaaa acccgcgaag
     2041 ttgagcccga ctccgacgca caggagcagc agcgggcggc tctgcgcgag catgagatag
     2101 ttgcgcgcga gcgggccaat ttcgaacggc tggcgctgcg tagcaggatg cgtctcgggc
     2161 aggtagcccc agcacaccac gaggagcacg agcgcgtaac cggcgaggaa caagaacacg
     2221 gcctgccagc caaacgccga ctgcagccat ccgcccacca ctggcgcgac cgcgggcgcg
     2281 agcccgaaga tcatggtcac gagcgacatc aggcgctgcg cgtcgtggcc ctcgaagctg
     2341 tcgcggatca cggcgcggcc cacgaccatc ccggccccgg ccgatgtccc ctgcagtacc
     2401 cggaaaaaga gcagatgcgc gaaatcggtc gccgccgcac atcccagcga tgccgcgatg
     2461 tacacgagca ggcttgccag gatcaccggc cggcgtccga acgagtcgga aagcgtgccg
     2521 tggaacagcg tcatcactgc gaacgtcaaa aagtacacgc tgagcgtctg ctgcagctgc
     2581 gcgggtgtcg cggcgaggtc ccgtcccatc gcggggaagg acggcagata ggtgtcgatc
     2641 gtgaaagggc cgatcatcgc gagaccggcg agcatggccg ccaggaacgg catggagacc
     2701 gcggagcggc gcacgtaact gcctttcgtt agggttatga aaatggacgt ctgctgccgt
     2761 agcccgatta taaggttcgg gcttgaacgc cccgcacgaa gagggcggtg ggagcgtcga
     2821 ggagacgctc ggctcaccgc ccatgtccgc tcccgcatta cgagagcgcc ttaagcgcgc
     2881 gattgcgcgc cggatttacc cgcgtctgcg accgcgccgg ccgcggcgcg actgcgcttc
     2941 ctcggcgctg ctgccattgc cgcccgcata ttgcgcttct tccgccgcct gctgcgtggt
     3001 ctggcgcaac tggtccagcg tttcctcggt gcggcggctc acttcctcga ccccttggcg
     3061 cagctgctcg gccacttccg ccgtttgctg ctccacgagt tgaccgacct gctgctgcac
     3121 gagactcatg gtctcgctcg tgtgacgcat gacattgagc gcctgctggg cgccggccac
     3181 gaagagctca
//
LOCUS       DASURG010000112         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_124299, whole genome shotgun sequence.
ACCESSION   DASURG010000112 DASURG010000000
VERSION     DASURG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124299"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..2828
                     /gene="ligD"
                     /locus_tag="VFY80_01880"
     CDS             30..2828
                     /gene="ligD"
                     /locus_tag="VFY80_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6006680.1"
                     /translation="MPRLARSARPIPTPARPRRAKRAPTGIQNATLNEYSAKRAFEAT
                     PEPEPRAGAPGSGPLLFVIQRHSARRLHYDFRLECDGVLKSWAVPKGPSPDPREKRLA
                     VQTEDHPYDYASFEGVIPPKQYGAGEVIVWDCGVYSPDEDGKYWFGDRKEAERRVRAG
                     LEKGKLSFMLRGEKVKGSYALVRTRDGKNWLLMKHKDRFASTVDLTEQNRSVLSGMTV
                     EALKAQAVTRLPASQLVPSGSVKPMPQSIAPMHAEPGEAAFDHPDWMWEPKLDGYRVI
                     AVVRPDGVRLCSRRGLDLTPAFPRLRHELIAQNASMIVDGELVAFDADGKPSFNALQN
                     RVQLKSAREIDAADQKSPVLFYCFDLLHFAGIDLTDRPYRDRRRYLSQCLLPTPLVKL
                     VHTTEEGLALQNAAVASGFEGVVGKRKDSKYQAGRRSSAWLKVKPTTSAEFVVGGYTK
                     GKGAREPLGALLVGYWENGTLHYASHVGSGFDTRMLASVKARLEKLRRETCPFKEKPD
                     LHNPTTWVEPEMVVETKFHSWTADGRLRAPVLLRVRDDLDPKSVRRRDERPVAAATTG
                     ERRKPPSSATELEDVLRQLATSKATLTLQVGPHAIRLTHLDRVYWPADEALEQPQLTK
                     RDLLRYFAQVSPYILRHLADRPLTMIRMPEGIRGQRFFQKHWRERRPAFAETVTVYSE
                     SKDESHEYLVCNNLPTLLWLAQSGTLEFHVWHSRARLGTDTPVQSTDYASSLEALEGS
                     ILNYPDYVVFDIDPYIYSGKEARGAEPELNVTAFEKGKEVAFWLRELLQSMALEPIVK
                     TSGKTGLHVFVPIARTIDFDAARQVSEIIGRHLMRRHPGVITMEWSVPKRTGKIFMDY
                     NMNVRGKTLNVAYSPRGVPGAPVSMPLAWEALERAHPLDFRITNAIERLDQSGDRWAD
                     ALERKQSIEHALDRTQRR"
     gene            2832..>3181
                     /locus_tag="VFY80_01885"
     CDS             2832..>3181
                     /locus_tag="VFY80_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6006681.1"
                     /translation="MAARSIASLTVSFGLVSIPVKLYSATEASRAISFNLLHKTCGSR
                     LRQQYICIKEDVVVPRDEMVKGYEFAKDQYVMFTPEELKAMEEAGTHTAEITEFVPIE
                     AIDPVYFDKAYYLGP"
BASE COUNT          634 a         1009 c         1001 g          537 t
ORIGIN      
        1 acgcatcgaa atcactcctt atccgaatca tgccgcgtct cgcccgttca gcgcgtccga
       61 tcccaacgcc cgcccgtccg cggcgagcaa agcgcgcacc gaccggtatt cagaacgcaa
      121 cgcttaatga atactccgcc aaacgcgctt tcgaggcgac cccggagccc gaaccgcggg
      181 cgggcgcgcc gggcagcggg ccgctcctct tcgtcatcca gcgccattcc gcgcgccggc
      241 tccattatga cttccggctc gaatgcgatg gagtgctgaa atcgtgggcc gttcccaaag
      301 gaccttcgcc tgatccgcgc gagaagcggc tcgcggttca aaccgaggac catccgtatg
      361 attacgcctc cttcgagggc gtcattccac cgaagcaata cggcgccggc gaagtgatcg
      421 tatgggactg cggcgtgtac tctccggacg aagatggaaa gtactggttc ggcgaccgca
      481 aggaggccga gcggcgcgtg cgggcggggc tcgagaaggg caagctcagt ttcatgctgc
      541 gcggcgagaa ggtgaagggc tcgtatgcgc tcgtgcgcac gcgcgacggc aagaactggc
      601 tcctgatgaa gcataaggac cggtttgcat cgaccgtgga cctgaccgag caaaaccgct
      661 cggtgctttc gggtatgacg gtggaggcgc tgaaggcgca ggccgtcacc cgattgcccg
      721 cctcgcagct cgtgccgagc ggatccgtca agcccatgcc gcagtcgatt gcgccgatgc
      781 acgcagagcc gggcgaggcg gccttcgacc atcccgactg gatgtgggag cccaagctcg
      841 atggctatcg cgtgatcgcc gtcgttcgcc cggacggcgt gcgcctctgt tcgcggcgcg
      901 ggctcgatct tacgccggcc ttcccgcgac tgcgccacga gctgatcgcc cagaacgcga
      961 gcatgatcgt cgatggcgag ctggtcgctt tcgatgccga tggcaagcct tcgttcaacg
     1021 cgttgcagaa tcgtgtgcag ctcaaatccg cgcgtgaaat cgatgccgcg gatcaaaagt
     1081 cgcccgtact cttttattgc ttcgacctgc tccacttcgc cggaatcgat ctcaccgacc
     1141 gcccataccg ggatcgcaga cgctacctct cgcaatgcct gctgcctaca ccgctggtga
     1201 agctcgtgca cacgaccgaa gaggggctcg cgctgcagaa cgccgccgtt gcgagcggat
     1261 tcgagggcgt ggtgggaaaa cgcaaggaca gcaagtatca ggctggacga cgttcgtccg
     1321 cgtggctcaa ggtaaagccg acgacgagcg cggaattcgt agtgggtggg tatacgaagg
     1381 gaaaaggcgc acgcgagccg ctcggcgcat tgctcgtcgg ctattgggaa aacggcacgc
     1441 tccattacgc atcgcacgtc ggatcgggat tcgacacgag gatgctcgcg agcgtgaaag
     1501 cccggctcga gaaactgcgg cgcgaaacgt gccctttcaa ggaaaagccg gacctgcaca
     1561 acccgacgac ctgggttgag cccgaaatgg tggtcgagac gaaattccac agctggaccg
     1621 ccgacgggcg gttgcgcgcg ccggtgttgc tgcgcgtacg cgacgatctc gatccgaaga
     1681 gcgtgcgccg gcgggacgaa cggcccgttg ccgcggccac gaccggcgag cgaagaaaac
     1741 cgccgtccag cgcgactgaa ctcgaagacg tgctgcgaca gctcgcgacg tcgaaggcga
     1801 cgctgacgct tcaggtcggc ccgcacgcca ttcgcttgac gcacctcgac cgcgtgtact
     1861 ggccggcgga tgaggcgctg gagcagccgc aattgacgaa gcgggatctt ctgcgctact
     1921 tcgcgcaggt ctcgccctac atccttcggc atcttgccga tcgcccgctc acgatgatcc
     1981 gcatgccgga agggatccgc ggacagcgct tctttcaaaa gcactggcgc gagcggcgtc
     2041 ccgcattcgc cgagacggtc accgtctatt ccgaaagcaa ggatgaatcc cacgaatatc
     2101 ttgtctgcaa caacctgccg acgctgctct ggctcgcgca gtccggaacg ctggaatttc
     2161 acgtctggca ctcgcgcgcg aggttgggca ctgacactcc ggtgcagtca acggactatg
     2221 caagttcgct cgaggcgctg gaaggctcaa tcctcaacta tcccgactat gtcgtcttcg
     2281 acatcgaccc ctatatctac tccggcaagg aggcgcgcgg ggcggagccc gagctcaacg
     2341 tgaccgcgtt cgagaaaggc aaggaagtcg ccttctggct ccgcgagctg ctgcagtcca
     2401 tggcgctcga gccgatcgtg aaaacctcag gcaagaccgg gcttcatgtc ttcgtgccca
     2461 ttgcgcgcac catcgatttc gatgccgcaa ggcaggtgag cgagatcatc ggccggcacc
     2521 tgatgcgccg gcatcccggc gtcatcacca tggagtggag tgtgccgaag cgcacgggaa
     2581 agatcttcat ggactacaat atgaacgtcc ggggcaagac gctgaacgtc gcgtactccc
     2641 cgcgcggcgt gcccggcgcg ccggtgtcca tgccgctcgc atgggaggcg ctcgagcggg
     2701 cgcatccgct cgatttcaga atcacgaacg ccatcgagcg gctcgaccag agcggcgacc
     2761 gctgggcaga cgcactcgag cgcaagcaga gcatcgagca tgcgctcgac cgcacccaaa
     2821 ggagatagac catggctgca cgatccatcg catcgctgac cgtgtcgttc gggctcgtgt
     2881 cgatcccggt aaagctctat tccgccacgg aggcgagccg cgcgatttcg ttcaacctgc
     2941 tgcacaagac atgcggctcg cgcctccgcc agcagtacat ctgcatcaag gaagacgtgg
     3001 tcgtcccgcg ggatgagatg gtgaagggct acgagttcgc gaaagaccag tatgtcatgt
     3061 tcacgccgga agaattgaag gcgatggaag aggcgggcac ccataccgca gaaatcacgg
     3121 agttcgtgcc gatcgaagcg atcgaccccg tgtacttcga caaggcgtac tacctcgggc
     3181 c
//
LOCUS       DASURG010000113         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_226699, whole genome shotgun sequence.
ACCESSION   DASURG010000113 DASURG010000000
VERSION     DASURG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226699"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFY80_01890"
     CDS             <1..198
                     /locus_tag="VFY80_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006485536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX6006682.1"
                     /translation="IMSGKMLYEWLARKRGEPSATEAARYIDAAMERVIGDAGALTRD
                     LGGTASTAQMGDAVAMAIDRV"
     gene            complement(274..1719)
                     /locus_tag="VFY80_01895"
     CDS             complement(274..1719)
                     /locus_tag="VFY80_01895"
                     /inference="COORDINATES: protein motif:HMM:NF023211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006683.1"
                     /translation="MRSATKWSASMLLFVVAASMAWGQPQGEGTLLENPHYNFVQSYS
                     GTATCLQCHEAQAKHVFSSVHYQWKGDAPNLTNAKGRRIGKINSTNDFCTNPAISWIA
                     ILTNDDGKVIGNGCSKCHAGLGARPTEEMSRAQLENIDCLICHAPNYRREVVKKGDGA
                     LAWVPTAANNKDLMLNIAQNVVRPTNELCLRCHVGSGGGLNYKRGDLETAHLRPTRDF
                     DVHMGSGMQCIQCHQFENHRVKGGGTLMGGMDIPNDQRPQCENCHTGQVHKDATLNRH
                     TAAVYCTTCHIPSFARKDPTDMHRDWSKSELVKGEGRYEPAIELKSNVTPVYAWWNGN
                     GTIALLDEPVKKQANGKVAMYKPDGSIADPKARIYAFKYHTGRLPVELSSGLMIPVQV
                     GIVFRTANNMQAVKAGGKNFRKRDIPDADIGWIDTERYMGLFHEVQPKGKALACNDCH
                     MGGKRLDWKALGYKDDPMKAGGRRVKASAKG"
     gene            1952..3445
                     /locus_tag="VFY80_01900"
     CDS             1952..3445
                     /locus_tag="VFY80_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6006684.1"
                     /translation="MSKPITAQTIAEHAVERVLMLVDGKWVGSAEGHLIGIENPANHA
                     VVAEVPRGGAEDVDRAVHAAARAFDTWKAIAPRERGKLLWKIADAMEAASEPLARLLA
                     TETGNAIRTQSRPEIRTAIDVFRYFGGLAGETKGETVPLGENVLSYTRREPIGVVGAI
                     IPWNSPVVLGSLKIAMSIAAGNTLVLKAAEDAPLAILEVARLSQEHLPHGVLNVLTGY
                     GEECGAALAQHPLVRKLSFTGSTEVGKLVMRAASERIVPVSLELGGKSPAIVFPDADD
                     ERVVDGVISGMRFTRQGQSCTAGSRLLLHRTIFDSFLQKLVAKLAALKVGDPLEEATD
                     MGAIINRKQFERVCGYIEDGLRQKDARLVIGGLPPKEGPLAQGYFVQPTVFAEVRNEW
                     RIAREEIFGPVLAAIPWTEESEAIRMANDSHYGLAAYVWTHDIARALRTAHAIEAGWV
                     QVNQGLGQSLGHSYGGMKQSGIGREFSLEGMLESFTQRKSVTVNLQY"
     gene            complement(3490..>3970)
                     /locus_tag="VFY80_01905"
     CDS             complement(3490..>3970)
                     /locus_tag="VFY80_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006685.1"
                     /translation="GWSFLGVNAQLFGSGGQPAEEEQWSWIEENLARLGGHPIALFLH
                     KPLFLDHPEEPDHEDLTLRQSVLDHASRTRLVALCRRFGVRLVSCGHKHQSRSFSLNN
                     IYHVWSPATACVNGRPDALHWGVREVGFVEYRFNADRFTHRFVGSDFLFRHENYMRK"
BASE COUNT          707 a         1212 c         1282 g          769 t
ORIGIN      
        1 atcatgtcgg gcaagatgct ctatgaatgg ctcgcgcgaa agcgcggcga gcccagcgcg
       61 accgaagccg cccgttacat cgatgccgcg atggaaagag taatcggcga tgcaggcgcg
      121 ctgactcgcg acctcggcgg gacggcaagc accgcacaaa tgggcgatgc ggtcgcgatg
      181 gctatcgatc gggtttaaag actagcggac gatcgcacac cgaacgccgc cgtgcgcggc
      241 gctcggtcag gagccggtac atcgttcggg aggctagccc tttgcgctcg ctttgacgcg
      301 gcgcccgcct gccttcatcg ggtcgtcctt gtagcccagc gctttccagt cgagccgctt
      361 gccgcccatg tggcagtcat tgcacgcgag cgccttgccc ttcggctgca cttcgtggaa
      421 gaggcccatg tagcgctcgg tgtcgatcca gccgatgtca gcgtcgggga tgtcgcgctt
      481 gcggaaattc ttgccgccgg ccttcaccgc ctgcatgttg ttcgcggtgc ggaatacgat
      541 gccgacctgc accgggatca tcaatccgga agacagctcg accggcagcc gcccggtgtg
      601 gtacttgaac gcgtagatgc gcgccttggg gtcggcgatc gagccgtcgg gcttgtacat
      661 cgcgacctta ccattcgcct gtttcttcac gggctcgtcg agtagcgcaa tcgtcccgtt
      721 gccgttccac catgcataga ccggcgtcac gttgctcttc agttcgatcg ccggttcgta
      781 gcggccttcg cccttcacca gctcggattt cgaccagtcg cggtgcatgt cggtcgggtc
      841 cttgcgcgcg aagctcggga tgtggcaggt ggtgcagtag accgcggcgg tatggcggtt
      901 caatgtcgca tccttgtgca cctgccccgt atggcagttt tcgcactgcg gccgctggtc
      961 gttcggaatg tccatcccgc ccatcagcgt gccgccgccc ttcacgcggt gattttcgaa
     1021 ctggtggcac tgaatgcatt gcatcccgct gcccatgtgc acatcgaagt cgcgcgtggg
     1081 acgcaggtgc gcggtctcga gatcaccgcg cttgtagttg aggccgccac ccgacccaac
     1141 gtgacacctc aaacacagct cgttcgtcgg gcgcacgacg ttttgcgcga tgttgagcat
     1201 gaggtctttg ttgttggccg ctgtgggcac ccacgcgagt gcgccgtcgc ccttcttcac
     1261 cacctcgcga cggtagttcg gcgcgtgaca gatgaggcag tcaatgtttt ccaactgtgc
     1321 gcggctcatc tcctcggtgg gtcgtgcgcc gagaccggcg tggcatttgg agcagccgtt
     1381 gccgatcacc ttgccgtcgt cattggtcag gatcgcgatc cagctgatcg cagggttggt
     1441 gcagaaatcg ttggtgctgt tgatcttgcc gatcctgcgc cccttggcat tggtcagatt
     1501 gggggcatcg cccttccact gatagtggac cgaggagaag acgtgcttcg cctgcgcctc
     1561 gtggcactgg aggcaggtcg ccgttccgct gtagctttgg acgaagttgt agtgcggatt
     1621 ctcgagcaga gtgccttcgc cttgcggctg gccccacgcc attgatgcag ccacgacaaa
     1681 cagaagcatg gacgccgacc actttgtcgc gcttcgcatg attgttctcc ttagcgtggg
     1741 gcacactgaa gataggtcgt gaagatttat atcaggatcg cctgaattct tcgttgacgc
     1801 atatcaaaca ccgcgcatca gacaggtttg ttttggctta tcagggaaat aggggacacg
     1861 gcgcgatcgc gagaggtgca ttgaactccc ggaatcctgt atcctcgtac gtttctagtc
     1921 gagcgcggca acgcgctccg tggaggcgct catgtcgaag ccgattaccg cacaaacgat
     1981 tgccgagcat gccgtcgagc gcgtgctcat gctggtcgac ggcaagtggg tgggcagcgc
     2041 cgaaggccac ttgatcggaa tagagaatcc ggccaaccac gccgtcgtcg ccgaagtgcc
     2101 gcgtggcggc gccgaagatg tagaccgtgc agtgcacgct gcggcgcgcg cgttcgatac
     2161 gtggaaagcc atcgcaccgc gcgagcgcgg caagctcctg tggaagatcg ccgatgccat
     2221 ggaggcggca agcgagccgc tcgcccggct gcttgcgacg gagaccggca acgcgattcg
     2281 cacgcaatcg cggcccgaaa tccgtacggc cattgacgtc ttccgctact tcggcggtct
     2341 cgcgggagaa accaaaggcg agaccgtgcc gctcggggaa aacgtcctca gttacacgcg
     2401 acgggaaccg atcggcgtgg tcggcgcgat cattccgtgg aattcgccag tcgtgctcgg
     2461 ctcgctcaag atcgcaatgt ccatcgcggc gggaaacacg ctcgtgctga aggcggcgga
     2521 ggatgcaccg ctcgcgatcc tcgaagtcgc acgcctttcc caggagcatc tgccccatgg
     2581 ggtgctcaat gtgctgactg gctacggcga ggagtgcggg gcggcgctcg cgcagcatcc
     2641 gctcgtgcgc aagctttcgt tcaccggctc gactgaagtg gggaagcttg tgatgcgcgc
     2701 cgcctcggaa cggatcgtgc ccgtttcact ggaactcggc ggcaagagtc cggcgatcgt
     2761 cttccccgac gccgacgacg agcgggtggt ggacggggtg atcagcggca tgcgcttcac
     2821 ccggcagggc cagtcgtgca cagcgggctc gcgcctttta ctccatcgta cgattttcga
     2881 ttccttcctt cagaagctcg tcgcgaagct cgcggcgctc aaggtgggtg atccgctcga
     2941 ggaggcaacc gacatggggg ccatcatcaa ccgcaagcag ttcgagcgcg tctgcggcta
     3001 catcgaggac gggctgaggc agaaggatgc aaggctcgtg atcggcgggc tgcccccgaa
     3061 ggagggaccc ctggcgcaag gctacttcgt tcagccgacc gtgttcgccg aggtgcgcaa
     3121 tgaatggcgc atcgcgcgcg aagagatttt cggtccagtg cttgccgcca tcccgtggac
     3181 cgaagagagc gaagcgatcc gcatggcgaa tgacagccac tacgggctcg cggcctacgt
     3241 ctggacgcac gacatcgcgc gcgcgctccg cacggcacat gccatcgaag ccggatgggt
     3301 gcaggtgaac cagggactgg gccagtcgct cgggcattct tacggcggca tgaagcagag
     3361 cggcatcggg cgcgaattct cactcgaagg catgctggag agcttcacgc agcggaaaag
     3421 cgtgaccgtc aacctccagt attaagcctt gcaggtcgtc cggaacgtgg tgagcgcacg
     3481 gacgcgcgct tatttcctca tgtagttttc gtgtcggaaa aggaagtccg aacccacgaa
     3541 gcgatgcgtg aagcggtcgg cgttgaagcg atactcgacg aaccccacct ctcgcacgcc
     3601 ccagtgcagg gcgtcgggcc taccgttgac gcaggcggtc gccggactcc acacgtgata
     3661 gatgttgttc agtgagaagg agcgcgactg atgcttgtgt ccgcagctca cgagccgcac
     3721 accgaagcgc ctgcagagcg cgacgagccg cgtccggctc gcatgatcga ggaccgattg
     3781 gcgcagcgtg agatcctcgt ggtccggttc ctcgggatga tccagaaaaa gcggcttgtg
     3841 caggaagagc gcgatcggat gtcctccaag ccgcgcgagg ttttcctcga tccatgacca
     3901 ctgctcttcc tcggccggct gtccgccgct gccgaagagc tgcgcgttga cgccgagaaa
     3961 gctccaaccg
//
LOCUS       DASURG010000114         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_737325, whole genome shotgun sequence.
ACCESSION   DASURG010000114 DASURG010000000
VERSION     DASURG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737325"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="tcuA"
                     /locus_tag="VFY80_01910"
     CDS             <1..971
                     /gene="tcuA"
                     /locus_tag="VFY80_01910"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6006686.1"
                     /translation="TGIEIRYESGARALTQAESGRVNGVVVQGPNGIETIYAKAVVLG
                     CGGFETNPAWRRLYLGDPWDHAKVRGSNFNYGEGLRMALDIGAMPWGHWGGCHATPIN
                     AEAPDYGVQHLTDKTNRLSYFLGVMINIDGRRWIDEGADINSFTYAKYGGLILKQPRS
                     LVYQIFDSKVLSLLEPRYSTSEPFRSDTLEGLVKQLKVDHTQAMKTLAEYNAACGHGG
                     PFNPAVLDGVHTEGIDPPKTNWAQKLDTPPYVAYPVTGGITFTFGGVKVNADAQVVAT
                     DWKPISGLYACGEMVGGLFHYNYPLGTGLMSGAVFGRIAGRSAARA"
     gene            complement(973..2088)
                     /locus_tag="VFY80_01915"
     CDS             complement(973..2088)
                     /locus_tag="VFY80_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006687.1"
                     /translation="MPLIIFFSIAIHSCYLGSKVVLSLFALEMGANQFTVGVLAALYA
                     LMPLALGIYSGRLADTRGMRLPLAIGAACTGLAMLTGFLWQQLMGLVAVSILVGAGFV
                     FFNVSIQNLTGAYGGPAHRTRNFAWLSIGYSVSAFIGPMFAGFSIDHAGHGLTFLFFS
                     FFTVIPLGILALKRSFGRVSAEHSEDGKRSALELLGIQPLRRLIIISGLCVASAELFA
                     FYVPVFAHSIGLSASTIGIILGAYAAAVFVSRFMLPRLLRTYRSEQIMFASMLLAACA
                     FAVFPLMRNVYPLMLAAFVIGVGTGVAQPVLMAVSYEKSPAGRTGEVTGLRLTANNIA
                     RVAIPLVSGAVGSALGAAPVFWLNALNLAFVSSLSRK"
BASE COUNT          455 a          679 c          714 g          316 t
ORIGIN      
        1 aaaccggcat cgagatccgt tacgaaagtg gcgcgcgcgc gctcacgcaa gccgaaagcg
       61 gccgcgtgaa cggcgttgtg gtgcaggggc cgaacggcat cgagacaatc tatgccaagg
      121 cggtggtgct cggctgcggc ggattcgaga ccaatccggc atggcgccga ctgtacctgg
      181 gcgatccctg ggaccacgcc aaggtgcgcg gctccaactt caattacggc gagggcttaa
      241 ggatggcgct ggatatcggc gccatgccgt ggggccactg gggcggctgt cacgcgacgc
      301 cgatcaatgc cgaggcgccc gactacggcg tgcagcatct caccgacaag accaatcggc
      361 tgtcgtactt tctcggcgtc atgatcaaca tcgacggtcg gcgctggatc gacgaaggcg
      421 ccgacatcaa ttccttcacc tacgcaaagt acggcgggtt gatcctcaag cagccgcgga
      481 gcctcgtcta ccagatcttc gacagcaagg tattgagcct gctcgagccg cgctattcga
      541 cgagcgagcc gttcaggtcc gacacgctcg aagggctcgt caagcagctc aaggtcgatc
      601 acacgcaggc gatgaagaca ctggcggaat acaacgcggc gtgcggtcac ggcgggccct
      661 tcaatccggc cgtgctcgac ggcgtgcata ccgaagggat cgatccgccg aagaccaact
      721 gggcgcagaa gctcgatacg ccgccctatg tcgcgtatcc cgtgacgggc ggcatcacgt
      781 tcaccttcgg cggggtgaaa gtgaacgcgg atgcgcaggt cgtcgcgacc gactggaagc
      841 cgatctccgg cctctacgcg tgcggcgaaa tggtgggcgg cctcttccac tacaactatc
      901 cgctcggcac gggcctcatg tcgggcgcgg tcttcgggcg catcgcgggc cgcagcgccg
      961 cgcgggcgtg agctacttgc gggagaggga gctcacgaac gcgaggttga gcgcgttcag
     1021 ccagaagacc ggcgcagcgc ccagcgccga gccgacagcg cccgacacaa gcggaatcgc
     1081 cacgcgcgcg atgttgttcg cggtcaagcg cagtcccgtc acttcgccgg ttcggcccgc
     1141 gggcgatttt tcgtacgaca ccgccataag caccggctgc gcgacgccgg tccccacgcc
     1201 gatcacgaac gccgcgagca tcagcggata gacgttgcgc atcagcggaa agaccgcgaa
     1261 cgcgcatgcg gcgaggagca tcgaggcgaa catgatctgc tcggagcgat acgtgcgcag
     1321 caggcggggc agcatgaagc ggctgacgaa caccgccgcc gcgtaagcgc cgaggatgat
     1381 gccgatcgtc gaggccgaga gcccgatcga atgcgcgaag acgggtacgt agaacgcgaa
     1441 aagctcggcg gaagccacgc acaacccgct gatgatgatg agccggcgca gcggctggat
     1501 gccgaggagt tcgagcgcgc tcctcttgcc atcctcgctg tgctccgcag aaacccgtcc
     1561 aaaggaccgc ttgagcgcca gtatcccgag cggaatcacg gtaaagaacg agaagaagag
     1621 aaaggtgagc ccgtggccgg cgtggtcgat ggaaaacccg gcgaacatcg gcccgataaa
     1681 ggcggagacc gagtagccga tcgaaagcca cgcgaaattc cgcgtccgat gcgcgggccc
     1741 gccgtacgcg cccgtcaggt tctggatcga gacgttgaag aacacgaatc ccgcgcccac
     1801 caggatcgat acggccacga ggcccatcaa ctgctgccag aggaaccccg tgagcattgc
     1861 gagccccgtg cacgccgcac cgatggcgag cggcagccgc atgccgcgcg tgtcggcgag
     1921 ccgtccggaa tagataccga gcgcgagcgg catgagcgcg tagagcgctg cgagcacacc
     1981 gacggtgaac tgattcgcgc ccatctccaa ggcgaagagc gaaagcacca ccttgctgcc
     2041 gagatagcag ctgtggatcg cgatgctgaa gaagatgatg agcggcatcg tcctggaaag
     2101 tcatccggtc gccatgagtc tcgccgcgct cacttggcta tctagtgtgg tgtcccattg
     2161 ataa
//
LOCUS       DASURG010000115         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1043724, whole genome shotgun sequence.
ACCESSION   DASURG010000115 DASURG010000000
VERSION     DASURG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043724"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFY80_01920"
     CDS             complement(<1..668)
                     /locus_tag="VFY80_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6006688.1"
                     /translation="MDETKVLAAYEPLFRPKTVAVVGASTKGTALPNVFIRRIREFGY
                     DGAIYPIHPTAQEIDGLPAYKSLGETPEPIDYAYIAIAAAQVPPLLAGSKGRVRFAQV
                     ISSGFGEVDEGKALQEALAVSARTGGMRLIGPNCLGIYTPRGRVTFAEIGPREVGEVG
                     VISQSGGLGTDIIRRGLNRGLKYSGLITVGNCADITPTDILEFYLADPQTRVIGLYIE
                     TAKDG"
     gene            complement(674..1402)
                     /locus_tag="VFY80_01925"
     CDS             complement(674..1402)
                     /locus_tag="VFY80_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6006689.1"
                     /translation="MDQDGGGSRVMMPAQIFDRARQAGRTALDEPSGKALLATYGIAV
                     PRSLVVSGVGDIEAALDELAPPYVVKVISPDILHKSDAGGVKIGLQNAAEVAQAIRTM
                     MGQPVISESRIEGFLVEEMVPAGQEIVIGGVRDPDFGPLVMVGLGGIFVEILADVAFR
                     ICPITRIDAEEMLAELKGAAILEGARGHRPVPKAAIVDALLKMGGEAGLLMRHAGEIR
                     EADVNPLIVSENGACAADARFVLT"
     gene            complement(1416..2771)
                     /locus_tag="VFY80_01930"
     CDS             complement(1416..2771)
                     /locus_tag="VFY80_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6006690.1"
                     /translation="MFTKVLVANRGAVAARVLRALYELRIKSVAVYSDADYGAPYLEM
                     ASETQGIGPGPAAESYLNQDRVLDAITRCGADGVHPGYGFLSENAGFAQRVIEAGVRF
                     IGPSPKWIDAMGHKTRAREIAAEHGLPLSEGTDVLPAEPEAILAAARRIGFPVLVKPA
                     GGGGGIGMLPARNESELLSAVERSRSLAERGFGNAEVYLERLIERPRHVEFQVLGDQH
                     GAAVHLFERDCSTQRRNQKVIEEAPAPAIARSALDTMAEKAAAMVRGMGYDNIGTVEM
                     LYGAEGSFSFLEMNTRLQVEHGVTEAVTGVDLVHAQIRSADGERLTDILPTNLGLKGH
                     AIQARIYAEDPKTFFPSPGRLKVFRLPEDKTIRVETGYAEGRDVTPFYDPMIAKIIVH
                     ESTRERAIERLCETLKAVDIQGIKHNTPAVIGVLESSAFRDAEVHTGLIPQLMAQRKA
                     A"
     gene            complement(2782..>3499)
                     /locus_tag="VFY80_01935"
     CDS             complement(2782..>3499)
                     /locus_tag="VFY80_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX6006691.1"
                     /translation="LPESRTQVYDMRRIVKCIADKDTVFELKPRFGKSAVTALARLGG
                     KSVGIVANNPLHRGGALDADACRKIVDFLVLCDSFNVPIVLLVDTPGFQIGTEAEKAG
                     APGRIMNFMNAMTLVTVPRLSVIIRKSYGRAYVCMGGGRHSDDVAAWPTAEVSFMSPE
                     FATKIVHGVGAGEPGFEEKFAQIRKGSDVWGLAAVYAAQAVIRPEQTRDYLNRMLDVY
                     RLRITKGVGQHLMRTWPTSY"
BASE COUNT          578 a         1199 c         1095 g          627 t
ORIGIN      
        1 ccgtccttcg ccgtctcgat atagaggccg atcacgcgcg tctgcgggtc cgcgaggtaa
       61 aactcaagaa tgtccgtcgg cgtgatgtcg gcacaattcc cgacggtgat gagtcccgag
      121 tacttgaggc cgcggttcaa gccccggcgg atgatgtcgg tgccgagacc gccgctctgc
      181 gagatgacgc cgacctcacc cacttcgcgc ggcccgatct cggcgaacgt cacgcgcccg
      241 cgcggcgtgt aaatgccgag gcagttcggg ccgatgagcc gcatgccgcc cgtgcgcgcg
      301 gacaccgcaa gcgcctcctg cagggccttg ccttcgtcga cctcgccgaa gccgctcgag
      361 atcacttgcg caaagcgcac ccgtcccttg ctgccggcga gcagcggcgg cacctgcgcg
      421 gccgcgatcg cgatgtaggc atagtcgatg ggctccggcg tctcgcccag actcttgtat
      481 gcaggcaagc cgtcgatctc ttgcgcggtg gggtgaatcg gatagattgc gccgtcataa
      541 ccgaattcgc ggatgcggcg gatgaacacg ttggggagcg ccgtgccctt ggtcgatgca
      601 ccgaccaccg caacggtctt tggccggaag agcggctcgt aggccgccag gaccttcgtc
      661 tcgtccatgt gcgtcacgtc agcacgaagc gcgcatccgc cgcacaggcg ccgttctcgg
      721 aaacgatgag cggattcacg tccgcctccc tgatttcgcc cgcgtggcgc atgaggaggc
      781 cggcctcgcc gcccatcttg agcagcgcat cgacaatcgc cgctttgggc accggcctgt
      841 ggccccgtgc gccctccagg atcgcagcac ccttcagttc agcgagcatc tcttcggcgt
      901 cgatgcgcgt gatcggacag atgcgaaatg cgacatcggc gaggatctcc acgaagatgc
      961 cgcccaggcc gaccatcacc aaagggccga aatcgggatc gcgcacgccg ccgatcacaa
     1021 tctcctgccc tgccggcacc atctcttcca caagaaagcc ttcgatccgc gattcggaaa
     1081 tgacgggctg ccccatcatc gtgcgaatcg cctgcgcgac ttcagcggcg ttttgcagac
     1141 cgatcttcac accccccgca tcgctcttgt gcaggatgtc aggcgagatc actttgacga
     1201 cgtagggcgg tgcgagctcg tccagcgcgg cttcgatgtc gcccaccccg gacacgacaa
     1261 gcgagcgcgg caccgcgatg ccgtatgtcg cgagcagcgc cttgccggac ggctcgtcga
     1321 gcgccgtgcg gccggcctgc cgcgcgcgat cgaagatctg agcgggcatc atcacgcggc
     1381 tgcctccgcc atcttgatcc atgttgctcc gccgctcatg cggctttcct ctgcgccatc
     1441 agctgcggaa tcaggccggt atgcacttcg gcgtcgcgaa acgcgctcga ctccaggacg
     1501 ccgatcaccg caggcgtgtt gtgcttgatg ccttggatgt ctacggcctt cagcgtctcg
     1561 cacagccgtt cgatcgcgcg ctcgcgggtg gactcgtgaa cgatgatctt cgcgatcatg
     1621 ggatcgtaga acggggtgac atcccgtcct tccgcatagc ccgtctcgac acggatcgtc
     1681 ttgtcctccg gaagacggaa gaccttgagt cggccgggcg agggaaagaa cgtcttcggg
     1741 tcctccgcat agatgcgtgc ctggatcgca tgccccttga ggccgaggtt cgtcggcagg
     1801 atgtcggtca gccgctcgcc gtcggcactg cggatctgcg cgtgcacgag atcgacgccc
     1861 gtcaccgctt cggtgacgcc atgctcaacc tgcagtcgcg tgttcatttc gagaaagctg
     1921 aacgagccct cggcgccgta gagcatctcg accgtgccga tgttgtcgta gcccatgccg
     1981 cgcaccatcg ctgccgcttt ctccgccatg gtatcgagcg cgctccgcgc aatcgccggc
     2041 gcgggcgctt cctcgatcac cttctggttg cggcgctggg tcgagcagtc gcgctcgaag
     2101 agatgcaccg ctgcgccgtg ctggtcgccg agtacctgga actccacgtg gcgcggccgc
     2161 tcgatcagcc gctcgagata gacctcggca ttgccgaagc cgcgctccgc gagcgagcgc
     2221 gaacgctcca ccgcgctgag aagctcggat tcgttgcgcg ccgggagcat cccgatgccg
     2281 ccgccgccgc ctgcgggctt gaccagcacc ggaaagccga tgcggcgcgc ggcggcgagg
     2341 atggcctccg gttccgcggg gagcacgtcg gtcccttcgg agagcggaag accgtgctcc
     2401 gccgcgattt cgcgtgcgcg cgtcttgtgc cccatcgcgt cgatccattt gggcgacggg
     2461 ccgatgaagc gcacgcccgc ctcgatcacg cgctgtgcga atccggcatt ctccgaaaga
     2521 aagccgtagc cgggatgcac gccgtccgcc ccgcagcgtg tgatcgcgtc cagcacccgg
     2581 tcctgattga gatagctttc cgcggcgggg cccggaccga tgccctgcgt ttcgctcgcc
     2641 atctcgagat agggggcgcc gtaatccgca tccgaataca cggcgaccga cttgatccgc
     2701 aattcgtaga gcgcccgcaa aacgcgcgcc gcaactgcgc cgcggttcgc taccaaaacc
     2761 tttgtgaaca tgagtagcgc ctcagtagct cgtgggccag gtgcgcatga ggtgctgccc
     2821 cacgcctttc gtgatgcgca gcctgtacac atcgagcatg cggttgagat agtcgcgcgt
     2881 ctgctcgggc cggatgacgg cctgcgcggc atacaccgcg gcaagccccc acacgtcgct
     2941 tcccttgcga atctgcgcaa acttttcctc gaagccgggc tcgcctgccc ccactccgtg
     3001 cacgatcttg gtcgcaaatt ccggagacat gaagctcacc tccgccgtgg gccacgctgc
     3061 gacgtcatcg gagtgccgac cgccacccat gcacacatag gcgcggccgt aactcttgcg
     3121 gatgatgacg gagagccgcg gcaccgtgac gagcgtcatc gcattcatga agttcatgat
     3181 gcgacccggg gcgccggcct tttccgcttc ggtgccgatc tggaacccgg gcgtatcgac
     3241 gaggagcacg atcggcacgt tgaacgaatc gcacagcacc aggaagtcca cgatcttgcg
     3301 gcacgcatcc gcgtcgagcg cgccgccgcg atgcagcggg ttgttcgcca cgatgccgac
     3361 gcttttcccg ccgaggcgcg cgagggccgt caccgcgctc ttgccgaagc gcggtttcaa
     3421 ttcgaatact gtgtccttgt cggcgatgca cttcacgatg cgccgcatgt cgtagacctg
     3481 cgtgcggctt tccggaagg
//
LOCUS       DASURG010000116         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1248499, whole genome shotgun sequence.
ACCESSION   DASURG010000116 DASURG010000000
VERSION     DASURG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248499"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..814
                     /locus_tag="VFY80_01940"
     CDS             29..814
                     /locus_tag="VFY80_01940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6006692.1"
                     /translation="MKSKQQATWSSGDYAVIGTTLQIVGETLAEAVDLRAGERVLDVA
                     AGNGNATLAAARRFAHVTSTDYVQHLLDKGAARASAEGLDVAFRLADAEALPFEDGCF
                     DVLLSTFGALSTPDHGKTARELCRVVRRGGRIGMANWTPEGFIGELFRVIGTYLPAPI
                     GLESPALWGSEPHIARLFGPLATELCCVRRRFRFRYRSAAHWIQVFRNFYGPIHKAYE
                     GLDPEHQSHLTADITALLERSNVGGPHSLVVPSEYLEVVIRKQ"
     gene            888..1523
                     /locus_tag="VFY80_01945"
     CDS             888..1523
                     /locus_tag="VFY80_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6006693.1"
                     /translation="MPMKLYVTPGSPYARMARMVVLEKGLSSRVETVIAQTRTPDSPY
                     YRINPSGRVPYLVRDDGVGLEESAVICDYLDRLDGKPAFLLPDGEALWEARRLEALAR
                     SMLDGIAVWGRELARPPNERSPTTLRHEAERSRRMADLWEREIDHPWMHGALNMAQIT
                     LGCALGFEARMPELAWRPDHPTLCAWFQRIASRPSFAATAPPGPVPAPAGR"
     gene            complement(1537..1839)
                     /locus_tag="VFY80_01950"
     CDS             complement(1537..1839)
                     /locus_tag="VFY80_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006694.1"
                     /translation="MTYFGLTEWGAINWAIVIIALGLVAGATRSVMRVLPERRMMRCP
                     QTGSITLLEIEKRDGRIAVTQCGLWPQREECSRGCLARYSEASGDFSTRLRALRPF"
     gene            complement(1967..2872)
                     /locus_tag="VFY80_01955"
     CDS             complement(1967..2872)
                     /locus_tag="VFY80_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6006695.1"
                     /translation="MAVDFAQGGVSEVADGIFVYVGPDGDSNNGIVLTGEGTISIDSY
                     VRQYSGLMRALEEVSDRPVRLAINTHDDADHYSMNHFFRRQGAMIIASEVCRSRIETK
                     MAMSSWVEDLRRRNPGLAHELTRPEELVPHVGLQDRATLNISGERIELMHMGHGHCPG
                     DLVVNFPERGVLFAGDLVFAGVHGRLKTADVDTLIAMLERLALIPCKTVVPGHGLPVK
                     GVGKEAIESYRDYLIVLRGRIAELILNGVTAERVATELEGWKYAKWGRGKLFPVCAQH
                     VYKDVAWRLRFSLDDPAQIMTQAGA"
     gene            3058..3261
                     /locus_tag="VFY80_01960"
     CDS             3058..3261
                     /locus_tag="VFY80_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6006696.1"
                     /translation="MSVAKVIEISAESNQSFEHAIRDGIATASKTVRNIRSAWVKEQQ
                     VKVDNTGKITSYRVDLKLTFVLD"
     gene            complement(3269..>4055)
                     /locus_tag="VFY80_01965"
     CDS             complement(3269..>4055)
                     /locus_tag="VFY80_01965"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006697.1"
                     /translation="ATARWLGIFLGPGVGGGLMLALGPAHGILINALIYLPIVLWLWK
                     APYGPRFRKEPRAPVKPVRGLGEIAATIRDVSGNRIIVSMILLAGSAAFFVGHGYQPQ
                     MPEFAHDLGRDHAGALYSMLLGANAAGAFIAGIALESRGLLPPNPRTALVLAVVWCLA
                     IVGFATSTVYVVALPLLFVAGFLELSFNAMAMTLVQLHAPASMRGRVIGLYNMAALGM
                     RAFSGVTIGVIGGLIGIHWSLALSAVVLLAIILTLFAFSTRPA"
BASE COUNT          765 a         1341 c         1257 g          692 t
ORIGIN      
        1 cacacctgca acggtcgacc tcaccgcggt gaagtcgaag caacaggcga cgtggagctc
       61 cggtgactac gcggtcatcg gcaccacgtt gcagatcgtc ggcgaaacgc tggcagaagc
      121 ggtcgacctg cgagcgggag aacgcgtcct cgatgttgcg gccggcaacg gcaacgccac
      181 cctggccgct gcccgtcgct tcgcgcatgt gacgtccacc gactacgtcc agcatctgct
      241 cgacaagggc gccgcgcgag caagcgccga agggctcgat gtcgcatttc ggctggctga
      301 cgcggaagcg ttgccgttcg aagatggatg tttcgacgtg ttgttatcga cattcggtgc
      361 actgtctacc ccggaccacg gcaagacagc gcgcgagtta tgccgcgtgg ttcgccgcgg
      421 tgggcgcatc ggtatggcga actggacgcc ggaggggttt atcggcgaac tgtttcgggt
      481 tatcggcacc tacctgccgg cgcccatagg cctggagtcg ccggcgctgt ggggctcgga
      541 gccgcacatt gccaggttgt tcggcccgct cgcgaccgag ctttgctgcg tgcgacgccg
      601 attccgcttc cgataccgct ccgccgcgca ttggatccaa gtattccgca acttctacgg
      661 accgatacac aaggcgtacg aaggactcga tcccgagcac cagtcgcact tgaccgcgga
      721 tatcacggcg cttctcgagc gcagtaacgt gggcggacct cactctctcg tcgtgccaag
      781 cgaatatctg gaagtcgtga tcagaaagca atgaagaggg ctcgcgcgct taccggaatt
      841 gaagaaagtc actgcgggtt ggtcttctga tatatgctgc cggcagcatg cccatgaagc
      901 tttacgtcac gcccggctcc ccgtacgcac gtatggcgcg catggtggtg ctggaaaaag
      961 gcctctcgag ccgggtggag acggttatcg cgcagacacg gacgccggac agcccctatt
     1021 accgcatcaa tccgtcgggg cgcgtcccgt atctcgttcg cgacgacggc gtgggcctcg
     1081 aagaatcggc agtgatatgc gactacctcg accgcctcga cggcaagccg gcattccttc
     1141 tccccgatgg agaggcgctc tgggaagcgc gccgccttga agcgctcgcg cggagcatgc
     1201 tggacgggat cgcggtgtgg ggacgtgagc ttgcgcggcc gcccaacgag cgctcgccga
     1261 ccacgctacg gcacgaagcg gagcgcagcc ggcggatggc cgatctctgg gagcgggaga
     1321 tcgatcaccc gtggatgcac ggtgcgctca acatggcgca gatcacgctc ggctgtgcgc
     1381 tgggattcga ggcgcgaatg ccggaactcg catggcggcc ggatcatccg acgctctgcg
     1441 cgtggttcca gcgcatcgca tcgcgtcctt ctttcgcggc cacggcaccg cctgggcccg
     1501 taccggcccc tgccggccgc tgatctgccg cacccgctag aacggtctca gcgcgcgtag
     1561 tctggtcgaa aaatccccgc tcgcttccga gtagcgcgca aggcatccgc gactgcactc
     1621 ctcgcgctgc ggccacagcc cgcactgcgt caccgcgatc cggccatcgc gcttctcgat
     1681 ctccaatagc gtgatcgacc ctgtctgcgg gcacctcatc atccgccgtt ccggcaaaac
     1741 cctcatcaca gaacgcgtcg cgccggccac gagtcccagt gcaatgatca cgatcgccca
     1801 gttgatcgcg ccccattccg tcagtccaaa gtacgtcata ttggcatcct cgtgacactt
     1861 ggatacgccc aatatgcaac ctgcggacga tggcggattt gacttagatc aatgtggcgc
     1921 gcggttcgtg ctgtgcttgg aaacacgggt cgactcgaag agcccctcat gcaccggcct
     1981 gcgtcatgat ctgtgcgggg tcatcgaggc tgaagcgcag ccgccatgcg acgtccttgt
     2041 agacgtgctg cgcacatacg gggaacagtt ttccgcggcc ccatttggcg tatttccatc
     2101 cttccagctc ggtcgcgacg cgttcggcgg tcacaccgtt aaggatgagc tcggcgatgc
     2161 ggccgcgcag cacgatgagg taatcccggt aactctcgat cgcttccttg ccgacgcctt
     2221 tcaccggcag gccgtgtccc ggcaccacag tcttgcaggg gatcagcgcg agccgttcca
     2281 gcatggcgat cagggtatcc acatccgcgg tcttgagccg gccgtgcacg cctgcgaata
     2341 cgaggtctcc ggcaaagagc acgccgcgct cggggaagtt gacgacgaga tcacccggac
     2401 agtgcccgtg ccccatgtgc atgagctcga tcctttcgcc ggagatgttg agtgtcgcgc
     2461 ggtcctgcag gccgacatgc ggcaccagct cctccgggcg ggtgagctcg tgggcgagcc
     2521 cgggattgcg ccgacgcagg tcttccaccc acgagctcat cgccatcttc gtctcgatgc
     2581 gcgaacggca cacctcggag gcgataatca tcgcgccctg gcgcctgaaa aagtggttca
     2641 tcgagtaatg atccgcgtcg tcatgggtgt tgatcgcgag ccgcaccggc cgatcgctca
     2701 cctcctccag agcgcgcatc aatccggaat actgcctgac gtagctgtcg atcgagatcg
     2761 tgccctcgcc cgtcagcacg atgccgttgt tggaatcgcc atccggaccc acgtagacga
     2821 atatcccgtc tgcgacctcg cttactcccc cctgcgcaaa atcgacggcc atcgttgcat
     2881 ctcctccggt ttactgcagg cgccacgcct gctcttacgc caaaatacgc cgcccgcttg
     2941 gcagcgcgca agaccgcatc gattccgccg ccgaaactcg gtcgatcgat gcgtgtccta
     3001 ccgatgcacg cactcgcgtg cccggcacta ccgccaaacc acccaaggag gatcgacatg
     3061 agcgtcgcca aagtcatcga aatcagcgcg gaatcgaacc agagcttcga gcacgcgatc
     3121 cgggacggca tcgcaacggc atccaagacc gtgcgcaaca ttcgcagtgc gtgggtcaag
     3181 gaacagcagg tgaaggtgga caataccgga aagatcacca gctaccgggt cgatctcaag
     3241 ctgacattcg tgctggattg accgcccgtc acgcgggacg cgtgctgaac gcgaacagcg
     3301 tgagaatgat cgccagcaga accacagcgc tcagtgcaag cgaccagtgg atgccgatca
     3361 gacccccgat gacgccgatc gtgaccccgc tgaaggcgcg cattcccagc gccgccatgt
     3421 tgtagaggcc gatcacacgc ccccgcatgc tcgcgggggc gtgtagctgc accagcgtca
     3481 tcgccatcgc attgaacgac agctcgagga atccggcgac gaagagcagc ggcagggcga
     3541 cgacatagac cgtcgaggtc gcaaacccga cgatggctaa gcaccagacg acggcgagca
     3601 cgagcgcggt gcgcgggttg ggagggagga gcccgcggct ttcgagcgcg atgcccgcaa
     3661 tgaacgcgcc cgcggcgttc gccccgagca gcatgctgta gagcgcgccc gcgtgatcgc
     3721 gcccgagatc gtgcgcgaac tccggcatct gcggctggta accgtgtccg acgaagaagg
     3781 cagcgctgcc cgcgagcagg atcatcgata cgatgatgcg attcccggag acatcgcgga
     3841 tcgtcgccgc gatctcgccc agtccccgca ccggcttcac tggagctctg ggctccttgc
     3901 ggaagcgcgg accgtacggc gccttccaca gccatagcac gatcggcagg taaatcagcg
     3961 cgttgatgag tatgccgtgc gcgggaccga gcgcgagcat gagaccgccg cccacaccgg
     4021 gccccaggaa aatgccgagc caccgggccg tcgcg
//
LOCUS       DASURG010000117         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1351001, whole genome shotgun sequence.
ACCESSION   DASURG010000117 DASURG010000000
VERSION     DASURG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351001"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1061)
                     /locus_tag="VFY80_01970"
     CDS             complement(78..1061)
                     /locus_tag="VFY80_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006698.1"
                     /translation="MKRLSSVVFAVALMAACPIVLAQKPQAAASYPTKPIRMIVPFAP
                     GGGTDIIGRLVAQELGQAWGQTVVVDNRGGGGGTIGTHLAARATPDGYTMVLCSLGVS
                     YAPALYSKLPFDPLTDLAPISLVATQPFVYVVVPMLGVNSMKDLIALAKAKPGQIRYG
                     SGGAGGSSHLGTELLRTMTGIDIVHVPYKGTGPALTAMLSGEIQMQLIGISSVVPHMK
                     SGRMRALAVSGAKRSAAAPDVPTVAESGVPDYAFDVWYGMLFPAGTPRAIVAKTNAEI
                     NRALKSPALAQRFAGVGVEPAGTTAEQFGDMLKREIAKWRKVVADAKIRIE"
     gene            1444..>2028
                     /locus_tag="VFY80_01975"
     CDS             1444..>2028
                     /locus_tag="VFY80_01975"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6006699.1"
                     /translation="MTEATDDVRVRGIEALTPPAEVLREAPLTLAAKNVVAETRQALH
                     RILHGADDRLITIIGPCSIHDVRAAREYGTRLREAKERLSDQLVVLMRVYFEKPRTTV
                     GWKGLINDPQLDGSFRINEGLRLARRLLLDLNESGVPVACEFLDMVTPQYIADLVSWG
                     AIGARTTESQIHRELASGLSCPVGFKNGTDGNVRI"
BASE COUNT          371 a          675 c          629 g          353 t
ORIGIN      
        1 ctggaccccc gctttcgccg gggcgacgtc atactccggc tttagcccgg ctaacgaaac
       61 tgtctatgtg cggaacgtta ttcgatgcga atcttcgcat ccgccacgac cttgcgccac
      121 ttcgcgattt ctcgcttcag catgtcgccg aattgctccg ccgtggttcc ggcgggctcg
      181 acgcctacgc ccgcgaagcg ctgcgccagc gcgggcgact tgagcgcgcg attgatctcc
      241 gcattcgtct tcgcgacgat cgcgcgcggc gtgccggcgg gaaacagcat gccgtaccac
      301 acatcgaatg catagtccgg gacaccgctc tccgcgacag tcggcacatc gggtgcggcg
      361 gcggagcgct tcgccccgct cacggcaagc gcacgcatgc gtcccgactt catgtgcggt
      421 acgaccgaag agatgccgat cagctgcatc tggatctccc cggacaacat cgccgtgagc
      481 gccggtcccg tgcctttgta cggcacatgg acgatatcga tgccggtcat ggtgcgcaga
      541 agctcggtgc cgagatgtga cgacccgccg gcgccacccg agccgtagcg aatctgcccg
      601 ggcttcgcct tcgcgagcgc aatgagatcc ttcatggagt tcacgccgag catcggcacg
      661 acgacgtaga cgaacggctg cgtggcaaca agcgagatcg gcgccaagtc tgtcagcgga
      721 tcgaacggca gcttggagta aagcgcgggc gcgtacgaca cgccgagact gcagagcacc
      781 atggtatagc cgtccggcgt cgccctggcg gcgagatggg tgccgatggt gccacccccg
      841 ccgccacggt tgtcgacgac gacggtctgt ccccacgcct gcccaagctc ctgcgcgacg
      901 agtcgcccga tgatgtcggt gcccccgccg ggagcgaacg gaacgatcat gcggatcggc
      961 ttggtcggat aacttgcggc ggcttgcggc ttctgcgcga gcacgatcgg gcatgccgcc
     1021 atgagagcca ccgcgaacac cacgctgctg agtcgtttca tgctctcctc tcgtattgcg
     1081 gcgctcctcg gtgggcgcgg cgcattatcg gttttcccgt cgcgggatac tagcggcctt
     1141 aagcaaaaac gccccggaag cggggcttcc ggggcgcggt agtgcagaca agccgcctca
     1201 gcggccgacg aacttcctct tgagcgggaa tgatccgtca cacgagccgt tgaagactgc
     1261 cttgtctaga tgggcttgca ccggatcacc tcctttcaaa gttgaccagc gcgcagtcta
     1321 gcagctcctt caaaaacggg aaatatggcg gatcgcctcc ctttacggcg ccccgcgata
     1381 tactcttacg cagtatcgcg tcactgtggc agctcccccg ggggaagggg aacagggatg
     1441 gacatgactg aagcgactga cgacgtccgc gtccgcggca tcgaagcgct cacgccgccg
     1501 gcggaagtac tgcgcgaagc gccgctcacg ctcgcggcca agaacgtcgt tgccgagaca
     1561 cgccaggcgc tccatcgcat ccttcatggc gccgacgatc gcctgatcac catcatcggt
     1621 ccttgctcca tacacgatgt ccgtgccgcg cgggagtatg gaacgcgcct gcgcgaagcg
     1681 aaagagcgcc tgtcggacca gcttgtcgtg ctgatgcgcg tctatttcga gaaaccgcgc
     1741 accacggtcg gctggaaagg gctcatcaac gacccgcagc tcgacggcag cttccgcatc
     1801 aacgaggggc tgagactcgc gcgtcgcctg ctgctcgatc tcaacgagtc gggcgtgcca
     1861 gtcgcctgcg aattcctcga tatggtgacg ccgcagtaca tcgccgacct cgtatcgtgg
     1921 ggcgcgatcg gcgcacgcac cacagaaagc cagatccatc gcgagctcgc atcgggtctg
     1981 tcatgcccgg tcggcttcaa gaacggaacc gacggcaacg tgcgcatc
//
LOCUS       DASURG010000118         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_431662, whole genome shotgun sequence.
ACCESSION   DASURG010000118 DASURG010000000
VERSION     DASURG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431662"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFY80_01980"
     CDS             complement(<1..232)
                     /locus_tag="VFY80_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX6006700.1"
                     /translation="MRIGIALGLAAGLLLGIGGYTFIYARGYSYLTDDPGACVNCHVM
                     QEQYSGWVASSHRSVAVCNDCHTPHDAIGKYVT"
     gene            complement(236..838)
                     /locus_tag="VFY80_01985"
     CDS             complement(236..838)
                     /locus_tag="VFY80_01985"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX6006701.1"
                     /translation="MPHSLPALPYDFGALEPHIDTQTMQIHHGKHHQAYVTNLNAALD
                     KHPELHNKSLDDLLRGLNDLPEDIRTAVRNNGGGHWNHSMFWKIMAPNAGGAPSGAVA
                     DGINSSFGSFDKFKEQFQAAGLARFGSGWAWLIDNNGKLEITSTPNQDNPLMEGKKAI
                     LGVDVWEHAYYLKYQNRRGDYLSAWWNVVNWNEVNRLLKG"
     gene            complement(915..>2062)
                     /gene="nadA"
                     /locus_tag="VFY80_01990"
     CDS             complement(915..>2062)
                     /gene="nadA"
                     /locus_tag="VFY80_01990"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX6006702.1"
                     /translation="TTFLPVLNEVEKASLHERQPLPEAYLGLSDEEMDARIAAARAAL
                     GARLVILGHHYQRDEVIKFADYTGDSFKLAQSIAKHPKADFIVFCGVHFMAESADILA
                     LPHQRVILPDLAAGCSMADMVAPDQLATCWSELEQMGLAARVIPVTYINSAAAVKAFV
                     GERGGTTCTSSNARASLEWAWAQKEKVLFLPDQHLGRNTAWKMGVPLDDMVVWDPYEP
                     FGGLTADELDRARLILWKGHCSVHVRFTAKQIEAVRQQYPGIRVIVHPEVPFEVMRVA
                     DDAGSTEYILRTVREAPAGTTWAVGTEIHLVHRLAEEMAPQKTVISLDQVGCLCSTMF
                     RVSPNHLLWVLEGLLDGSVHNEIVVPDEQQKWARIALERMLSIKGRS"
BASE COUNT          359 a          639 c          663 g          401 t
ORIGIN      
        1 tggtgacgta cttgccgatc gcatcgtgag gcgtgtggca gtcgttgcag acggcaacgg
       61 agcggtgact cgacgcaacc cagcccgaat actgctcctg catgacgtgg cagttgacgc
      121 aggcaccggg atcgtcggtg agatacgagt agccgcgcgc gtagataaag gtatagccgc
      181 cgatccccag caggagaccg gcggccagac ccagggcaat gccgatgcgc atcggctacc
      241 ccttgagcag cctgttcacc tcgttccagt tgacgacgtt ccaccaggcg ctcaagtagt
      301 cgccgcgccg gttctgatat ttcaggtagt aggcgtgctc ccagacgtcg acgccgagga
      361 tggccttctt gccctccatc agcggattgt cctggttggg tgtgctcgtg atctcgagct
      421 tgccgttgtt gtcgatgagc caggcccacc cgctgccgaa ccgggcgagc cccgccgcct
      481 ggaactgttc cttgaacttg tcgaagctgc cgaacgacga attgatcccg tcggcaacgg
      541 caccgctcgg cgcgccgccg gcgttgggcg ccatgatctt ccagaacatc gagtgattcc
      601 agtgtccgcc gccattgttg cgcactgccg tgcggatgtc ttcgggcagg tcgttgagcc
      661 cccgcagcag atcgtcaagg ctcttgttgt gcagttcggg atgtttgtcg agcgcggcgt
      721 tcaggttcgt gacgtaggcc tggtgatgct ttccgtgatg gatctgcatc gtctgcgtat
      781 cgatatgcgg ttcgagggcg ccgaagtcgt acggaagcgc cggcaagcta tggggcattg
      841 cggtctcctc gtgtgttgcg gattaaaagg tcgcggacct gcggtccgct cacaacgtga
      901 tcagggttgg cgcgtcagct ccgtcccttg atcgagagca tgcgctccaa tgcgatccta
      961 gcccatttct gctgctcatc ggggacgacg atctcgttgt gcacgctgcc atcgagcaga
     1021 ccttccagga cccagagcag gtgattcggc gagacgcgga acatcgtcga gcagaggcag
     1081 ccgacctgat ccagggagat cacggttttc tgtggagcca tctcttctgc aagacgatgg
     1141 acgagatgaa tttcagtgcc gaccgcccat gtcgtgccgg ccggcgcttc gcgcaccgtg
     1201 cgcaggatat actcggtcga tccggcgtcg tcggccacgc gcatgacctc gaacggcact
     1261 tccggatgca cgatgacgcg gatgcccgga tactgctgac gcacggcttc gatctgtttg
     1321 gccgtgaagc gcacgtgcac cgagcaatgc cccttccaga gaatcagccg cgcgcggtcg
     1381 agctcgtccg cggtgaggcc accgaacggc tcgtacggat cccacaccac catgtcgtcg
     1441 aggggaacgc ccatcttcca ggcggtgttc cgcccgagat gctgatccgg aaggaagagc
     1501 accttctcct tctgcgccca cgcccattcg agcgaggcgc gcgcgttcga cgaggtgcag
     1561 gtcgtcccgc cgcgctcgcc gacgaacgcc ttcaccgccg ctgccgagtt gatgtacgtg
     1621 accggaatga cgcgcgcggc aaggcccatc tgttcgagct cgctccagca agtcgcgagc
     1681 tgatccggcg cgaccatgtc ggccatcgag cagccggcgg ccaggtccgg aaggatgacg
     1741 cgctggtgcg gaagcgccag gatgtcggcg ctctcggcca tgaagtgcac gccgcagaac
     1801 acgatgaagt ccgccttcgg gtgtttcgcg atcgactgcg cgagcttgaa cgaatcgccg
     1861 gtgtaatcgg cgaacttgat gacctcgtcg cgctgatagt gatggccgag gatgaccagc
     1921 cgagcgccaa gcgctgctct tgccgcggca atgcgggcgt ccatttcttc gtcggaaagc
     1981 ccgagatacg cctccgggag cggctgacgt tcgtggaggc tggccttctc gacttcgttc
     2041 agaaccggca ggaaggtcgt cg
//
LOCUS       DASURG010000119         4967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_228737, whole genome shotgun sequence.
ACCESSION   DASURG010000119 DASURG010000000
VERSION     DASURG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228737"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..2256
                     /locus_tag="VFY80_01995"
     CDS             325..2256
                     /locus_tag="VFY80_01995"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX6006703.1"
                     /translation="MSIAARRVLIVLGVLLLVVPALLVAGAALILQSESTERWVEARI
                     GNALEREVEVEGIDLQLGWPPAINVEHLRIGNPDWAKTEHLIDATNLHARIELLPLFS
                     RRIVIPYFSARTATAGLEQDDGRATWRVGGGGEGESPFLVRRVNVEEGRILYRDAADN
                     TALDITLKGSLGEGGELDIAASGTFKGERAKGTARVPSLQPTPETPIQIEAKAAVGRT
                     KVTAEGNFAANLESIDMRLHVAGPTLRALEKLTAMKLPETPPFTVSGQLRHTGAEWIF
                     DPFQGKVGDSDLRGSLTLRTGGKRPALKADLQSKLLDFDDLGPLVGAPPNPEEKATPE
                     QKAKAAEQRAKEKVLPREPLGVDRWDAMDADVKLEAQRIVRPKQLPIDALATRIVLKD
                     AVLRLEPLNFGVAGGRVKATIVLNGQKNPVEGSVDLDAQGIKLSRVFPEKKGLEPSLG
                     TLYGRGKLNGRGASIGELLGTSNGQISLAVDGGLVNLLLVELLGLDVAESATLLGAGK
                     AQKVELRCAVADFSLKEGVATPETFVIDTTDTAVKVTGAVHLGEERLDLVTYPEPKDM
                     SLFSLRSPVQLQGAFKDPQVRPKAGPIVARGAAAAALAIANPLLALLPFIETGPGEDS
                     DCGALLSEVRAKGAVKKKE"
     gene            complement(2278..2877)
                     /locus_tag="VFY80_02000"
     CDS             complement(2278..2877)
                     /locus_tag="VFY80_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX6006704.1"
                     /translation="MHRNPGAQGELFGAGLPEGLLYVPDFLGEAEEASLIAAIQALPL
                     AEARYKQYTARRRIASFGSQYDFGENELRPAAPLPDFLKPLRVKLSRWLSLPAEAFSD
                     ALVAEYRPGTPLGWHRDVPEFAVITGVSLGASCRMRFRPYPPGVSRARNAFELCLEPR
                     SAYVMRDEARWRWQHAIPPTRGLRYSITFRTRSTRRSAA"
     gene            complement(2914..3600)
                     /locus_tag="VFY80_02005"
     CDS             complement(2914..3600)
                     /locus_tag="VFY80_02005"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HEX6006705.1"
                     /translation="MTLNPSVLAAAAQDGPANPDRYAERCIAEKRIPIFCCEGPCLEG
                     RIARRAVDIVTRALPSYGRAWHSEALLAPQSAMTRWIAGAEKAVMIEGCFLKCQAAML
                     DMLAGADKLVHIVAFPLDRRYAHSFQENVLSPGEIHELAEEAAERIMAALVSRPRAQP
                     AAPELAAQLKCWWPNCNTVLREDVSVMAAGEPSVEHDHTGYYITCPQCGARTYPPQAV
                     CSAMEAEGAG"
     gene            3771..4442
                     /locus_tag="VFY80_02010"
     CDS             3771..4442
                     /locus_tag="VFY80_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6006706.1"
                     /translation="MTESERYRRGVEILRQVGGPEFDTPLKALEDVAPDLVRLAVEFG
                     YGEIMSRPALDLPTRQLATVAALCAMGNAPSQLRYHIEGALNVGCPASHVTEVVLASS
                     GIVGSGLARDGLLAMNEVLRRRGLTPPIEVAAAEDPARYEARLDPRLDRRTKVLATIA
                     LLTALGTAHENLKLEVRAALDSGVTRVEIVEAIQQMALYAGFPAALNGIAAAKEVFAG
                     NTAVR"
     gene            complement(4452..>4967)
                     /locus_tag="VFY80_02015"
     CDS             complement(4452..>4967)
                     /locus_tag="VFY80_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006707.1"
                     /translation="WLFGLTALSALLGAIAHGFELTEKMREAIWVPLYLSLGIVVGLI
                     IVAAVYDWRGQNAAARLVIWTLMLGAGFFAVTQFVSGAFVVFLVYEAVALLCALGIYF
                     FLAAKRRLPGAGVIALAIALSMMAAAVQASDARVVWIVPFDHNGIFHLVQIGAVAMLA
                     AGLRQGLRPAR"
BASE COUNT          945 a         1617 c         1622 g          783 t
ORIGIN      
        1 cttgtcgacc gtccggttgt aggcgacctc gacctggttg ccgcccgcac cctgcgtgcc
       61 ggtgtcatcg gtgaccagcg gctggaacgc gtgcgctgcg cccgcaacgg aaagcgcgaa
      121 accgaaagcg atgcaccctg cacggaccat ttttctctcc cgccccatcg gcggattata
      181 cgtaggacgc tcgcgggatg cgctcgcggg atgcgctcgc gggaaccgtt gcagttctgc
      241 gcaatcatag tgattgcccc tgcccgcccg ggttgcattg cgccaccgct ccgttcccgg
      301 gccgccagcc tggagtccat catcatgtcg atcgcggcac ggcgcgtgct catcgttctg
      361 ggcgtgctgc tcctcgttgt cccggcgttg ctcgtcgcgg gcgcggcgct catcctgcaa
      421 tcggagtcca ccgagcgttg ggtggaagcc cgcatcggaa acgcgctcga gcgcgaagtg
      481 gaggtcgaag gcatcgatct tcaactcggc tggccgcccg cgatcaacgt cgagcacctg
      541 cgtatcggca atcccgactg ggccaagacc gagcatctca tcgacgcgac gaaccttcac
      601 gcccgcatcg agcttctgcc gctgttcagc cgccgcatcg tcattcccta tttcagcgcc
      661 cgcaccgcga cagcgggtct cgagcaggat gacggacggg ccacttggcg cgtggggggc
      721 ggcggagagg gcgaaagccc gtttctcgtg cggcgcgtca acgtcgaaga gggcaggatc
      781 ctctaccgcg atgccgccga caacacggcg ctcgatatca ccttgaaagg cagtctcggc
      841 gaaggcggtg aactggacat cgcggcgagc ggcaccttca agggcgaacg cgcgaaaggc
      901 actgcccgcg tgccgtcgtt gcagccgacg cccgagacgc cgatccagat cgaagcgaaa
      961 gctgcggtgg gacggaccaa ggtgacggcc gagggtaact tcgccgccaa cctcgaaagc
     1021 atcgacatgc gtctgcacgt tgccggaccg acgttgcgcg cgctggagaa gttgactgcg
     1081 atgaaacttc ccgagacgcc gcccttcacg gtcagcggac aactgcgcca taccggcgcg
     1141 gaatggatct ttgacccctt ccagggcaaa gtaggagaca gcgatctgcg cggaagtctc
     1201 acgctgcgca cgggcggcaa gcggcctgcg ctcaaggcgg atctccagtc gaaactgctc
     1261 gatttcgacg atctcggtcc gctcgtcggc gcgccgccca atcccgaaga gaaggcgaca
     1321 cccgagcaga aggcgaaagc cgccgagcaa cgcgcaaaag aaaaggtgct gccgcgcgag
     1381 ccgctgggcg tggaccgctg ggacgcgatg gatgcggacg tcaagctgga ggcgcaacgc
     1441 atcgtgcgac ccaagcagct tcccatcgat gcgctggcca cgcgtatcgt gctgaaagac
     1501 gcggtgctga ggctcgaacc gttgaacttt ggtgtggccg gtggacgcgt gaaggcgacc
     1561 atcgtgctca acggtcaaaa aaacccggtg gagggcagcg tggatctcga cgcgcagggg
     1621 atcaagctct cgcgcgtctt tcccgaaaaa aaagggctcg agccctcgct cggcacgctc
     1681 tacggccgcg gaaagctgaa cggccgcggc gcatcgatcg gggaactgct ggggacgagc
     1741 aacggccaga tcagcctcgc ggtcgacggc ggcctcgtca acctgctgct cgtggagcta
     1801 cttgggctcg atgtggcgga atccgcaacg ctgctcgggg caggcaaggc gcagaaggtc
     1861 gagttgcggt gcgcggtcgc ggacttttcg ctgaaggaag gcgtcgcgac accggagact
     1921 ttcgtgatcg acaccaccga caccgccgtc aaagtgaccg gtgccgtgca cttgggggag
     1981 gagcgcctag acctcgtcac ctaccccgag ccgaaggaca tgagcctctt ctctttgcgc
     2041 tctccggtcc agctccaagg cgcgttcaag gacccgcagg tgcgtcccaa agcgggaccg
     2101 atcgttgccc gcggcgccgc ggcggcggcc ctcgccatcg cgaatccgct gctcgcactg
     2161 ttgcccttca tcgaaaccgg tcccggagaa gacagcgatt gtggcgcgct gctctccgag
     2221 gtgcgcgcca agggtgcagt gaagaaaaag gaatgaagcc gtgcgcgtcc gcccgtttca
     2281 cgcggcgcta cgccgcgtgc tacgtgtgcg aaacgtaatg gagtagcgca gtccccgggt
     2341 cggcggtatg gcgtgctgcc agcgccagcg cgcttcatcc cgcatcacat aggcggagcg
     2401 cggctccaga cagagctcaa aggcgttccg cgcccggctc accccgggcg gatagggtcg
     2461 aaaacgcatg cggcacgacg cgccgagaga aacgccggtg atcacggcaa attccggcac
     2521 gtcgcgatgc caaccgagcg gcgtgcccgg gcgatactcg gcaaccagcg cgtcggagaa
     2581 tgcctccgcc ggtagcgaca accaccgtga gagcttcacg cgcagcggct tcagaaaatc
     2641 gggcagcggc gcggcgggtc tgagttcatt ctcgccgaaa tcgtactgcg acccgaagct
     2701 cgcgatgcgg cggcgcgcgg tgtattgctt gtaacgcgct tcagcgagcg gaagcgcctg
     2761 gatggctgca atgaggcttg cttcctcggc ctcgccgaga aagtcgggga catagagaag
     2821 gccttcggga aggcccgctc cgaaaagctc gccttgcgcg cccggattgc gatgcacgcg
     2881 ctcgacgaag caagcctcgt gccgagcgct ggtttacccg gcgccctccg cctccatcgc
     2941 cgagcagact gcctgaggcg ggtaggtccg cgcaccacac tgcgggcagg taatgtagta
     3001 gccggtgtgg tcatgctcga cggaaggctc gccggcggcc atcacggaca catcctcccg
     3061 cagcacggta ttgcagttgg gccaccagca cttgagctgc gcggcgagct ccggtgccgc
     3121 cggttgtgcg cggggacgac tcacgagggc cgccatgatc cgctcggccg cctcctccgc
     3181 cagttcgtgg atctcgcccg gcgagagaac gttttcctga aagctgtgcg catagcgccg
     3241 gtccagcggg aacgcgacga tatgaacgag cttatcggcc ccggccagca tgtcgagcat
     3301 tgccgcctga cacttgagga agcatccctc gatcatgacc gctttttcgg cgcccgcgat
     3361 ccaacgcgtc atggcagact gaggcgcgag cagcgcctcc gaatgccacg cgcgcccata
     3421 cgagggcagc gcccgtgtca cgatatcgac ggcgcggcgg gcgatccttc cctcgaggca
     3481 cgggccctcg cagcagaaga tgggaatcct tttttcggcg atgcagcgct cggcataacg
     3541 gtcggggttc gcgggaccgt cctgcgcggc agccgccaaa acggagggat tgagcgtcat
     3601 atctcacctc ggctctgaat cgacgcacgg gcttgctgca tcgtgagcgc gccgcgcgta
     3661 tgtcgctacc ctacacgtgc ggggaagcga cgccttgagc catatcaaca aaacgcgtga
     3721 cgcgccggcc atatgccaca atcgcggctg gtgagcggac gcgagcaggc atgaccgaaa
     3781 gcgagcgata tcggagaggc gtggagatac tgaggcaggt tggcggcccg gaattcgata
     3841 cgccgctcaa ggcgctggag gacgttgcgc ccgacctcgt tcggctggcg gtagagttcg
     3901 gttacggcga aatcatgtcg cggccggcgc tcgatcttcc gacccggcag cttgcgaccg
     3961 tcgccgcact ctgcgcgatg ggtaatgcgc cttcgcagct gcgctatcac atcgagggcg
     4021 cgctcaacgt cggttgcccc gcgtcgcacg tgacagaggt tgtcctggcc agcagcggca
     4081 tcgtcggatc gggcctggca cgagatggac tgctcgccat gaacgaggtc ttacgaagac
     4141 gtggattgac gccgcccatc gaggtcgccg ccgccgaaga cccggcacgc tacgaggcgc
     4201 ggctcgatcc gcgcctcgac aggcgcacaa aggtgctcgc cacgattgcg ctgctgaccg
     4261 cacttggaac ggcgcacgag aatttgaaac tcgaggttcg cgcagcgctc gatagcggcg
     4321 taacacgcgt cgagatcgtg gaagcgatcc agcagatggc gctctacgcc ggttttcccg
     4381 ctgcgttgaa cggcatcgcc gccgccaagg aagttttcgc agggaacacc gccgttcgtt
     4441 gaacgatccg cttatctcgc cggccgcagt ccctgacgca ggccggcggc gagcatcgcc
     4501 acggcaccga tctgaacgag atgaaaaatg ccgttgtgat cgaagggcac gatccacacg
     4561 acccgagcgt cactcgcctg cacggcggca gccatcatac tgagggcgat cgcgagtgcg
     4621 atcacaccgg cacccggtaa tcgacgcttc gctgcaagga agaaatagat cccgagggca
     4681 cagaggagcg ccacggcctc gtaaacgagg aatacgacga acgctccgct cacgaactgc
     4741 gtcaccgcaa agaagccggc ccccagcatg agcgtccaga tgaccaaacg cgccgcagcg
     4801 ttttgcccgc gccagtcgta aaccgccgcg acgatgatga gcccgaccac tattccgagg
     4861 ctcaggtaga gcggcaccca gatggcttca cgcatcttct cggtcaactc gaagccatgc
     4921 gcgatcgctc cgaggagcgc cgacaacgcg gtcagcccga atagcca
//
LOCUS       DASURG010000120         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_535097, whole genome shotgun sequence.
ACCESSION   DASURG010000120 DASURG010000000
VERSION     DASURG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535097"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFY80_02020"
     CDS             complement(<1..676)
                     /locus_tag="VFY80_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006708.1"
                     /translation="MTDTLKAARKRARTKSYPGSLTRRGDSWRLRLCVGGEYHSFTVA
                     GTKLEAQNFAVQKHAALTAELRRAVAGLPGPLRFSALLDEYERYELPTRSAGTQKSYK
                     SSFVAFREFFGGELRDPFVRDIRRSHVQTFLEWRRTYRIRTDHDVSGHTVARDRRVLH
                     RLFNYGVAKDHLDANPCALVRAPKADPRTPPILSPEQLDKLLAACDSNPMLRLYVLLL
                     AETGLRA"
     gene            complement(679..1680)
                     /locus_tag="VFY80_02025"
     CDS             complement(679..1680)
                     /locus_tag="VFY80_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6006709.1"
                     /translation="MTKKQELLALERIAKAVREYAKQQGMTFDALGEKLDISRATVYN
                     LAGSTTRRRAPRAVALPSGDVLSKVSALTGISVDWLLGFEVPQQRDARLVIGDFAEHL
                     RSSLEQAFDGPVSSAPDQRDGAPDDDGGNEWVRVYAQERGIIRFPESAGELIEKVQGA
                     YWQAQIYEVAQDKAPGFQTLGLMLREKMRELQHRKSEKRVFLMLGDMLNAARAAHAFA
                     AVLNSFDVGWGDIADLDAFPAGVAQYNQPDDAPPLYTPFGVSGGIRGAAYAFQEEDSD
                     RFSMFWVDRRTGEARFDAGLAGPVTDEPPEYPYDETPSAGFWATHSYDEYPMPPARS"
     gene            2080..>2586
                     /locus_tag="VFY80_02030"
     CDS             2080..>2586
                     /locus_tag="VFY80_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX6006710.1"
                     /translation="MLTASSSFADRDALTASIRAHADLFTLVQEDTGDRIRGRGNERR
                     GPCPIHGGERPNFTVNVNEGVFHCFTCGAGGDVFDYVRATRGLDFPAARAFLAQRLGL
                     SLDASAGRPLRLQRPPAVRRRSPGQEQRDGWTAELESLRTEGCMPCSPATVYAAVLQN
                     LTLEDVGAA"
BASE COUNT          415 a          848 c          844 g          479 t
ORIGIN      
        1 ccgcgcgaag tcccgtctcg gcgaggagca gcacgtacag gcggagcatg gggttgctgt
       61 cacacgcggc gagtagcttg tcgagttgct cggggctgag gatcggcggc gtgcgcgggt
      121 ccgccttcgg cgctctgacg agcgcgcacg ggttcgcgtc aaggtggtcc tttgcaacgc
      181 catagttgaa cagccgatgc agtacgcgcc ggtcccgcgc gacggtatgc ccgctcacgt
      241 cgtggtcggt gcggattcgg tacgtgcgcc gccactcgag gaaggtctgc acgtggctgc
      301 gacgaatgtc tcgcacgaac gggtcgcgca gctcgccgcc gaagaactcg cggaaggcaa
      361 cgaacgagct cttgtagctc ttctgggtgc cggccgatcg ggtcggcagc tcgtaccgtt
      421 cgtattcgtc gagcagggcg gaaaagcgaa gcgggccggg gagccctgcc acggcgcggc
      481 gcagttcagc ggtcaatgcc gcgtgcttct gtacggcgaa gttctgcgcc tcgagctttg
      541 tgccggcgac cgtaaagctg tggtactcgc cgccgacgca gaggcggagg cgccacgagt
      601 cgccgcggcg cgtgaggctg cctgggtagc tcttggtccg ggcgcgcttt cgcgccgctt
      661 tcagagtgtc agtcatgctc agctcctcgc tgggggcatc ggatactcgt catacgagtg
      721 cgtcgcccaa aatcccgctg acggcgtctc gtcgtatggg tactccgggg gttcgtctgt
      781 cactggcccc gcgagcccag cgtcgaagcg cgcttcgccg gtgcggcgat cgacccagaa
      841 catggagaat cggtcgctgt cctcctcctg aaaggcatat gctgcccccc ggataccacc
      901 cgagacgccg aatggcgtat aaagcggggg cgcgtcgtca ggctggttgt actgagcgac
      961 accagccggg aacgcatcaa gatcggcgat atcgccccat cccacatcga atgagttcag
     1021 caccgcagca aaagcatgcg cggcacgagc ggcgttcagc atatcgccga gcatgaggaa
     1081 cacgcgcttc tcgctctttc ggtgctgaag ctcacgcatc ttctctctca gcatcaaacc
     1141 gagcgtctga aagcccggcg ccttatcctg agcgacctca tatatctgcg cctgccaata
     1201 tgcgccctgc accttttcta tcagttcgcc cgcagactcg ggaaagcgaa tgatcccgcg
     1261 ctcctgggcg tagacgcgca cccattcgtt gccgccatcg tcatccggcg ccccgtctcg
     1321 ctggtcaggc gccgatgata ccgggccatc gaaagcctgc tcgagtgagg atcggaggtg
     1381 ctccgcaaag tccccgatga cgagcctcgc gtcccgttgc tgcggaacct cgaaccccag
     1441 gagccaatca acggagattc ccgtaagcgc ggacactttt gacagcacgt cgccactggg
     1501 gagtgcgacc gcacgcggtg cgcgtcgacg cgttgtcgag ccggcgaggt tgtagaccgt
     1561 cgcccggctg atgtcgagct tttcaccgag cgcgtcgaag gtcatgccct gctgcttcgc
     1621 gtactcacgc accgctttcg cgatgcgttc cagcgccagg agctcctgtt tcttggtcat
     1681 cgtcgccgcg tcgcacctca tctaacatgt tggactaatc tgtccagact tatctactcc
     1741 acgaatctag gcgaatgcgc tggtatttgc tagatacctc ttgtcggcgt gcatgacgtg
     1801 cgccgcctag ggaggcgcat tctgtgacat cggtactgct cgatacagcc gcagcggccg
     1861 agcgagtggg actggcccgg caaacgcttg cgcagctccg cgtgacgggt ggagggccgc
     1921 cgtttcggaa gctgggagct cgtgtgtttt accccgcggc ggaacttgac cggtgggtcg
     1981 agtcgcaccc gctccgcgac tccaccgccg acgcgagccg ctatcgccag cgggacgccg
     2041 ctgcggcatc tgccgccgat tcctgaccgc gaaggcgtcg tgctcaccgc ctcgtcatcc
     2101 tttgcggatc gcgacgccct gacggcgtcg attcgtgcgc atgcggacct gttcacgctc
     2161 gtgcaagagg ataccggcga ccgcatccgg ggccgcggga atgaacgtcg cggcccgtgc
     2221 ccgattcacg gcggtgagcg gccgaatttc acagtgaacg tgaacgaagg cgtcttccac
     2281 tgcttcacct gcggcgctgg cggcgacgtg ttcgactacg tgcgcgccac gcgtgggttg
     2341 gacttcccgg cggcgcgcgc cttcctggcg cagcgacttg gcctctcgct cgatgcctcc
     2401 gctggtcgcc cacttcgcct tcagcggccg cccgccgtgc ggaggcgctc ccctgggcag
     2461 gagcagcgcg atggatggac ggcggagctc gagtcgctgc gcacggaagg ctgcatgccc
     2521 tgctcgccgg ccacggtgta cgcggcagta ctccagaacc tgacgctcga ggacgtgggt
     2581 gccgcg
//
LOCUS       DASURG010000121         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1147897, whole genome shotgun sequence.
ACCESSION   DASURG010000121 DASURG010000000
VERSION     DASURG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147897"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1276)
                     /locus_tag="VFY80_02035"
     CDS             complement(164..1276)
                     /locus_tag="VFY80_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006711.1"
                     /translation="MSELAALLHVPGASIEAQDDFDAINELVLARGWGDGLPIVPPTE
                     ARVRPMLQYCDRPWDEPIAKIAPRYGEATPLRLAANAVMAGCEPRYFPLLMLAIEAMC
                     EEPFNLYGIQATTHLCAPLVIVNGPVAHELAINAGHNAFGPGARANATIGRAIRLALL
                     NIGGATPGTGDMSTFGAPSKFSYVVAENETASPWPPLHVERGYPALASTVTVVGAECP
                     HNVNDHESLTAEGVLTTIAGTMGIAGSNDVYYAAQPVVVMGPEHAHTVAQGGYSKADV
                     RRFLQAHAHLPLGKFSHENIERRLRVTWKARFTNAGPDAPVPLVQDADDIILIVVGGA
                     GKHSAYIPTFGATRSVTRPLKRRDGEYARSVEEFRT"
     gene            complement(1269..1490)
                     /locus_tag="VFY80_02040"
     CDS             complement(1269..1490)
                     /locus_tag="VFY80_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006712.1"
                     /translation="MAELRKRGLVAAVICSGPFLKLGQNQAKVFGVPDLPLIVIPHPL
                     GGLALDTVRERAAVAIPPLVQMIRERIGE"
     gene            complement(1521..1811)
                     /locus_tag="VFY80_02045"
     CDS             complement(1521..1811)
                     /locus_tag="VFY80_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006713.1"
                     /translation="MTGRVTLVVPEAPAPVAASAVRQRTLGADSVRLGVLDNSKGNAD
                     HLLRFVVEGVRREVAVASVVSLRKGSVSLPAPRDILDTLERDADVVVSAMAD"
     gene            complement(2047..>2281)
                     /locus_tag="VFY80_02050"
     CDS             complement(2047..>2281)
                     /locus_tag="VFY80_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6006714.1"
                     /translation="IETPRCTSCDECTQLSNVIFAYDANKQAYIARPESATYRELVEA
                     AESCQVSIIHPGKPRNPDEPGLAELMERARPFL"
BASE COUNT          382 a          783 c          737 g          379 t
ORIGIN      
        1 tgatgtgcag caggagccac gtcagcggct cggccgccat ggcgaccgaa ggccacaaca
       61 cgagcagaac acacgcgatg ccgcgtcgca tcccttcctc cccccacacg accttcagga
      121 ttgtaggccc cgccccgacc cgattcaacg cgcagggcgc ccgttacgtg cgaaattcct
      181 ccaccgagcg cgcatactcc ccgtcgcgtc gcttgagcgg ccgcgtcacc gagcgcgtcg
      241 cgccaaacgt cggtatgtaa gccgagtgct tgcccgcacc gcccactaca atgaggatga
      301 tgtcgtcggc atcctgaacg agtggaaccg gcgcatccgg accggcattc gtaaagcggg
      361 ccttccatgt tacgcgcaag cgccgttcga tgttctcgtg cgagaacttg ccgagcggca
      421 aatgcgcgtg cgcctgcagg aaccgcctga catccgcctt cgaatagccg ccctgggcga
      481 cggtgtgagc gtgctccggc cccatcacga cgaccggttg cgcggcgtag tacacgtcat
      541 tcgatcctgc gatgcccatg gtgccggcga tcgtcgtcaa gacgccttcc gcggtcaggc
      601 tctcgtggtc gttgacgttg tgcgggcact ccgcgcccac caccgtcacc gtgctcgcga
      661 gcgcgggata gccgcgctcc acgtgcagcg gcggccatgg gctcgctgtt tcgttctcgg
      721 ccaccacgta cgagaacttg gacggcgcgc cgaacgtgga catgtcgccc gtgccgggcg
      781 ttgcaccgcc gatgttcagt agcgcaagcc ggatcgcgcg gccgatggtc gcattcgccc
      841 gcgcccccgg cccgaaggcg ttgtggccgg cattgattgc caattcgtgc gccaccggtc
      901 cgttcacgat gacaagcggg gcgcagagat gggtggtcgc ctggatgccg tagagattga
      961 aaggctcttc gcacatcgct tcgatcgcga gcatgagcag cggaaaataa cgcggctcgc
     1021 agcctgccat gaccgcgttc gccgcgagcc gcaagggagt cgcttcgccg tagcgcggcg
     1081 cgattttcgc aatcggctca tcccacggac gatcgcagta ctgcagcatc gggcgcacgc
     1141 gcgcttccgt aggcggcacg atcggcaggc cgtcgcccca gcctcgtgcc agcacgagtt
     1201 cgttgattgc gtcgaaatcg tcctgcgctt cgatggaagc gccgggcacg tgcagaagtg
     1261 cagcgagttc actcaccaat gcgctcccgg atcatctgca ccagaggcgg gatcgcgact
     1321 gcggcgcgct cgcgcacggt gtcgagtgcg agtccgccca gcggatgcgg gatcacgatc
     1381 agcggcagat ccggcacgcc gaacaccttg gcctggttct gccccagctt gagaaagggc
     1441 cccgagcaga tgacggcggc gaccagcccg cgcttgcgca gctccgccat gtcgtggaca
     1501 ctccacgacg tgcaggagcc tcaatcggcc atcgcgctga cgaccacgtc cgcgtcgcgc
     1561 tccagcgtgt ccaaaatgtc gcgcggcgcg ggaaggctca cgctgccctt gcgcagggac
     1621 acgaccgacg cgactgcgac ctcgcggcgg acgccctcga cgacgaagcg cagcaggtga
     1681 tccgcgttcc ccttgctgtt atcgagcaca cccagacgca cgctgtccgc cccgagcgtg
     1741 cgctgccgca ccgcgctcgc cgccacgggt gccggcgcct ccggcacgac gagtgtcact
     1801 ctgccagtca tccttgttcc tcgaaaagaa gcgcgcgccg aagcgcgaga tcgtcgcgac
     1861 tgtagcgtac cgtctccggc acctgcaatc gaggcgcggc tctctcccgt gacccatgcg
     1921 gcacgcagtc gtcatgccgg gcgggcagcg ctcgacgcta catgggtatc ggcataaaac
     1981 cgggcaccgg gatggcgctt cagtcacctg ccatccgggc aagcaacttc ggatggcgct
     2041 ggactttcag aggaaagggc gggcgcgctc catgagttct gcgagccccg gctcgtcggg
     2101 gttgcgcggc ttgccgggat ggatgatgga gacttggcag ctctccgcgg cctctacaag
     2161 ctcgcgatac gtcgcggact cggggcgcgc gatgtaggcc tgcttgttcg cgtcgtaggc
     2221 aaagatgacg ttgctgagct gggtgcactc gtcgcagctc gtgcagcgcg gcgtctcgat
     2281 g
//
LOCUS       DASURG010000122         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_841054, whole genome shotgun sequence.
ACCESSION   DASURG010000122 DASURG010000000
VERSION     DASURG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841054"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..527)
                     /locus_tag="VFY80_02055"
     CDS             complement(192..527)
                     /locus_tag="VFY80_02055"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HEX6006715.1"
                     /translation="MTKRIPEDAWAMELHRFTNRNAGRATTLEVDSPDFGAQAQQTGF
                     PLRGITYDARDDRVLITLGDQASAERHLTHMISQATAIFLLVDLKGRDEALAIRQNQT
                     STLLRFTDL"
     gene            689..2422
                     /locus_tag="VFY80_02060"
     CDS             689..2422
                     /locus_tag="VFY80_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6006716.1"
                     /translation="MARSEANKGQRGLWELIVGWVGGRGPSLGELPPDLASAEATFSA
                     IVNIASEAIITTDESLRILNFNKGAEDIFGYQAGDIVGRFLNELIPARFHSTHDQHVR
                     NFARSGVPARRMGERRQISGLRKNGEEFPAEASISQLQIGDHKLFTVVLRDITDRRRR
                     ELMQTLLAQAGELLAGSLEIDVTLNSVARLIVPHVADWCVVYVCQDDGSLVREAIAHR
                     DPTIERRLHETSLGRNVSNAAHPVLQVIKTGETVVIGDMTHEEFLAMSETPEHAQALQ
                     DIGARCAIIVPMIARHETRGAIALFTGGERRPFDDDDVALAEDLAHRSALALDNARLY
                     RKAQSAIAARDDVLAIVSHDLGNPLSAIRIGTTLLLSGLSEEEKQEGGWKHIVGIRNS
                     AEQMERLIRDLLEVKRIEAGQLSVERDKLSVAPLVSEALELLANIAQGKDVQLRAQIP
                     ESMPAVYADRERILQTFSNLVGNAVKFTPAGGAVTISAKAREDDVVLTVSDTGAGIAP
                     EDLRHVFDRYWQAKARRRGRQGIGLGLVIVKGIVEAHGGRVWAESELGKGSRFSFSLP
                     VWDERYELLEE"
     gene            complement(2463..2753)
                     /locus_tag="VFY80_02065"
     CDS             complement(2463..2753)
                     /locus_tag="VFY80_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006717.1"
                     /translation="MNHHQEEQDRVAFDAFMLALRVLYVAFDLDVPATEFERPFQILR
                     ELADGRLTVASPGELAARFPEMSQFIYDRSSSIIESDRETAARILRGLNRAL"
     gene            complement(2755..>2986)
                     /locus_tag="VFY80_02070"
     CDS             complement(2755..>2986)
                     /locus_tag="VFY80_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX6006718.1"
                     /translation="GGIGENAPEVRARVLHGLEWMGLKLDEELNQRVVGGTEGCITTP
                     DSRLRAWVIPTDEELLIARDTARLVLKLEARY"
BASE COUNT          556 a          950 c          938 g          542 t
ORIGIN      
        1 accagctcgt cgtaattttc cgtgatgacg gcttccgccg tcggcaccga gggtgtgtgc
       61 acgcgcacga tatggagccg agccttgctc ctttctgcga tgcgcatagc cagaggcagc
      121 gccagctcgc tgaaatgcga gccgtccacc ggaagcagga tctctctgaa catggcggcc
      181 tcccgagaaa attacagatc cgtgaagcga agcagcgtac tggtctggtt ctgccggatg
      241 gcgagtgcct cgtcgcgtcc cttcaaatcg accagcagaa agatggccgt ggcctgcgaa
      301 atcatgtgcg tcaaatgccg ctccgcgctg gcctggtcgc ccagcgtgat cagcacgcgg
      361 tcgtcgcgcg catcgtacgt gatgccgcgg agcgggaacc ctgtctgttg cgcctgggcg
      421 ccaaaatcgg ggctgtccac ttccagcgtg gtggcccggc cggcattgcg gttcgtgaac
      481 cggtgcagct ccatggccca ggcatcctcg ggtattcttt tcgtcacgca cgcacctcct
      541 gcaaccgcgt agacgatgcc aagctaccag agcgccggtc ccggctctgt cgggatcgtt
      601 acgtaagcct gtgcggagaa cgctgatttt aggccggggt aatgctacat tccggcacca
      661 atctgcaccg tggactcaat ggatcgtgat ggcgcgaagc gaggcaaata agggacagcg
      721 cggactctgg gagctgatcg tcggctgggt aggtggtcgc ggcccctcgc tcggggagct
      781 gcccccggat ttggccagcg ccgaggcgac tttttccgcc atcgtcaata tcgcctccga
      841 agccatcatc accaccgatg aaagcctgcg catcctgaac ttcaataagg gtgcggaaga
      901 cattttcggg taccaggcgg gggacatcgt cggccgcttc ctcaatgagc tgattccggc
      961 ccgcttccat tccacccacg atcagcacgt gcgcaatttc gcgcgctccg gcgtgccggc
     1021 ccggcgcatg ggcgagcggc gccagatctc gggactgcgc aagaatggtg aggagtttcc
     1081 cgccgaagcc tccatttccc agctgcagat cggcgatcac aagctgttca ccgtcgtgct
     1141 gcgggacatc accgatcgcc gccgccgtga gctgatgcag acgctgctcg cccaggcggg
     1201 cgagctgctg gccggttcgc tcgagatcga tgtcacgctg aatagcgtgg cccgcctgat
     1261 cgtaccgcat gtcgctgatt ggtgcgtcgt ctacgtctgc caggatgatg gctcgctggt
     1321 gcgtgaggcg attgcgcacc gcgatccgac catcgagcgc aggctgcacg agacctcgct
     1381 cggccggaac gtcagcaacg cggcccatcc cgtcctgcag gtcatcaaga ccggagaaac
     1441 cgtcgtcatt ggcgatatga cgcacgagga gtttctggcc atgtcggaaa cgcccgagca
     1501 tgcgcaggcg ctccaggata tcggtgcgcg ctgcgccatc atcgtgccca tgatcgcgcg
     1561 ccacgagacg cgcggcgcga tcgcgctgtt cacgggtggc gagcgccggc cttttgatga
     1621 tgacgacgtc gccctcgccg aagacctcgc ccaccgttcc gccctcgcgc tcgacaacgc
     1681 gcggctctat cgcaaagcac aaagcgcgat cgccgcccgc gacgacgtac tcgccatcgt
     1741 ctcgcacgac ctgggcaatc ccctcagcgc gattcgtatc ggcaccacgc tgctgttgag
     1801 tgggctgagc gaggaggaaa agcaggaagg cggctggaaa cacattgtcg gcatcaggaa
     1861 ttctgccgag cagatggaac gtctcattcg agatctgctg gaagtgaaac gcatcgaagc
     1921 cggacagctt tcggtcgaac gcgacaaact gagtgtcgcg cccctcgtgt cggaagcgct
     1981 cgaattgctg gcgaatatcg cgcaaggcaa agacgtgcag ctgagggcgc aaatccccga
     2041 gagcatgccc gccgtgtatg ccgatcgcga gcgcattctg cagaccttct ccaatctcgt
     2101 gggaaatgcc gtgaaattca cgccggccgg cggcgcggtc acgatttcgg cgaaagcgcg
     2161 cgaggatgat gtcgtactta ccgtcagtga taccggcgcc ggcattgcac cggaagacct
     2221 gcggcacgtc ttcgatcgct actggcaggc caaggcgcgg cggcgtggcc gtcagggcat
     2281 cggtctgggg ctcgtcatcg tgaaggggat cgtagaagcg cacggcggcc gtgtgtgggc
     2341 ggaaagcgag ctgggcaagg gcagccgttt cagcttttca ttgccggtgt gggacgagag
     2401 gtacgaactg ctggaggagt agcttcaccg cctcggcggt gcagctctga gctctcgaag
     2461 aatcacagcg cacgattcaa tccacgcagt atccgcgcgg ccgtctcgcg gtcactctcg
     2521 atgatcgagc tcgagcgatc gtagatgaac tgactcatct ccgggaaacg cgctgccagc
     2581 tcgcccggag atgcgacggt caaccggcca tccgccagct cgcgcaggat ctggaacggc
     2641 cgctcgaact ccgtggccgg cacatccagg tcgaaagcca cgtacagcac ccgcaacgcc
     2701 agcatgaagg catcgaaggc gacgcggtcc tgttcctcct ggtggtgatt catatcagta
     2761 gcgcgcttcc agtttgagca cgagtcgcgc ggtgtcgcgc gcaatgagca gttcctcgtc
     2821 ggtgggtatc acccaggcac gcaggcggga atcgggcgtc gtgatgcagc cctccgtacc
     2881 gccgaccacg cgctgattga gctcctcgtc cagcttcagt cccatccatt ccaggccgtg
     2941 cagcacacgc gcccgcacct ccggcgcgtt ttcaccaatg ccaccc
//
LOCUS       DASURG010000123         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_126451, whole genome shotgun sequence.
ACCESSION   DASURG010000123 DASURG010000000
VERSION     DASURG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126451"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="VFY80_02075"
     CDS             complement(<1..2232)
                     /locus_tag="VFY80_02075"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6006719.1"
                     /translation="MEDHHLLEVQGVHVSRREDLRLITGAGKYASDWNLPGQLHAYFL
                     RSDRAHAKIVSINAEAAKKHPGVVRIYTGEDTVRAGYVKPMTMLTFKGKGGMEVRVPE
                     RPALAHGRVRCVGEPVAMVVAESALVAQDAAELIEVEYQDLPAVIDPEEALAPGAPQL
                     HDNVPGNLPFEFEAGDAAATEAAFARAAHVTRLKLEVTRVAPNPMEPRACLMAYDAKE
                     DRYTIHVHVQGVNMMRKQLAAYTNLPEDKIRVMARDVGGAFGQRSLAYPEYCAQMLAA
                     KDIGRPIKWIASRSEGFATDSHGRANFVTGELALDRDGKFLGMRLDWIAEMGAYLTPT
                     GPVSHLRNPSTCLTGVYRIPALYGRWRTALTTTNPIAAYRGAGRPDIAYVIERLVSAA
                     AAETRVDAAELRRRNFIPPEAFPYKTPTGSTYENADFPGVLEKALKLADWKGFGERRK
                     RSEQAGKLRGIGISTVIENTGAGLFPKDTVAMDVAADGTITAYTVSHSQGQGHETAFA
                     QIIAETLGVEFERVVLRQGDESRPLFGNHTGGSRSTVGAGSACHLAAKKLIEEGKALA
                     ALELGVEPSQVEYANGAFRSDHAERALTLADLAKAKTFSVQAEGSFSSTFPNGCHIAE
                     VEIDPATGVTEIASYVTVDDCGRVINHTIVEGQMHGGVAQGAGQVFGEHVVYERNSGQ
                     LLTGSFSDYYMPRAGLLREIRMDEHPTPSKVSPLGVKGMGESGCTASLAALTNAVLEA
                     LRPL"
BASE COUNT          348 a          738 c          754 g          412 t
ORIGIN      
        1 gagcgggcgc agcgcctcca gaaccgcgtt cgtgagcgca gcgagtgagg cagtgcaacc
       61 ggattcgccc atccctttca cgccgagcgg gctcaccttg gacggcgtgg gatgctcgtc
      121 catccggatc tcgcgcagga gccccgcgcg cggcatgtag tagtcggaga agcttccggt
      181 caaaagctgc ccggaatttc gttcgtagac gacatgctcg ccgaacacct ggcccgcgcc
      241 ctgcgcgacc ccgccgtgca tctggccctc gacgatggtg tgattgatga cgcgcccgca
      301 atcgtcgacg gtgacgtacg atgcgatctc cgtgacgccg gtcgccgggt cgatctcgac
      361 ttccgcgatg tggcagccat tggggaaggt ggagctgaag ctgccttcgg cctgcactga
      421 aaaggttttc gccttcgcca gatcggcgag cgtcaacgcg cgttccgcgt ggtcgctgcg
      481 gaacgcgccg ttcgcgtact cgacctgcga aggctcgacg ccgagctcca gcgccgcaag
      541 cgccttgcct tcctcgatca gcttcttcgc agcaagatgg caggcgcttc ccgcaccgac
      601 ggtggaacgc gaaccgccgg tgtgattgcc gaacagcggc cggctctcgt cgccctggcg
      661 cagcaccacg cgctcgaatt cgacgccgag cgtctcggcg atgatctggg caaaggcggt
      721 ctcgtggcct tggccttgcg agtgcgacac ggtgtacgcg gtgatcgtgc cgtcggcggc
      781 aacgtccatc gccaccgtgt ccttgggaaa gagccccgcg ccggtatttt cgatcacggt
      841 ggaaatgccg atgccgcgca attttccggc ctgttccgag cgcttgcgac gctcgccgaa
      901 gcctttccag tcggcaagct tgagcgcttt ctcgagcacg cccgggaaat cggcattctc
      961 ataggtggat cccgtcggcg tcttgtacgg aaacgcttcc ggcggaatga agttgcggcg
     1021 gcgcagttcg gctgcatcca cgcgggtctc cgccgcggcc gcactcacca ggcgctcgat
     1081 cacgtatgcg atgtcgggac gcccggcacc gcggtaggcc gcgatcgggt tggtggtcgt
     1141 gagcgcggtg cgccagcgcc cgtagagcgc gggaatgcgg tagacgccgg tcaggcaggt
     1201 gctggggttg cggaggtggc tcaccgggcc cgtcggggtc agataggcgc ccatttcggc
     1261 aatccagtcg agccgcatgc cgaggaactt gccgtcgcgg tcgagggcga gctcgccggt
     1321 gacgaaattg gcgcggccgt gcgaatcggt cgcgaagccc tccgagcgcg aagcgatcca
     1381 tttgatcggg cgcccgatgt ccttcgcggc cagcatctgc gcgcagtact cgggataagc
     1441 caggctgcgc tggccgaacg cgccaccgac gtcccgcgcc atgacccgga tcttgtcttc
     1501 gggaaggttg gtataggcgg caagctgctt gcgcatcatg ttcacgccct ggacgtggac
     1561 gtggatggtg tagcggtcct ccttggcgtc atacgccatc aagcaggcgc ggggctccat
     1621 cgggttcggc gcgacccgcg tcacttcgag cttgagccgc gtgacgtgtg cggccctggc
     1681 gaacgcagct tcggtcgcgg ccgcatcgcc cgcctcgaat tcgaacggca ggttgccggg
     1741 cacattgtcg tggagctgcg gagcgccggg cgcgagcgcc tcctcggggt cgatgacggc
     1801 gggcaggtcc tggtactcca cttcgatcag ttccgccgca tcctgtgcga cgagggctga
     1861 ttccgcgacg accatcgcca ccggctcacc cacgcagcgc acgcggccgt gcgcgagcgc
     1921 cgggcgttcg ggcacacgca cctccatgcc gcctttgccc ttgaaggtca gcatggtcat
     1981 cggcttcacg tagcccgccc gtaccgtatc ttccccggta tagatgcgga caacgcccgg
     2041 gtgcttcttc gccgcttcgg cgttgatcga gacgatcttc gcgtgcgcgc ggtcggaacg
     2101 caggaaataa gcgtgaagct gccccggcag gttccagtcc gaagcgtact ttccggcgcc
     2161 ggtgatgagg cgcaggtcct cgcggcgcga gacgtgcacg ccttgcactt ccaacagatg
     2221 atgatcttcc atgggcttcc tatcgtatta tc
//
LOCUS       DASURG010000124         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1455121, whole genome shotgun sequence.
ACCESSION   DASURG010000124 DASURG010000000
VERSION     DASURG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455121"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFY80_02080"
     CDS             <1..955
                     /locus_tag="VFY80_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006720.1"
                     /translation="IIAQIARDHAVNLIDIRLSQMEPTDLRGVPFRKGDFVEWSVPSL
                     LPDAERHGARGILFLDEITSAPPTVTAAAYQLILDRRLGDYRVPHGWVIFAAGNRYGD
                     RGVSYVMPTPLANRFTHYAIEPDLEDWVAWAHTAGIDERIVAFLRFRPDLLFQFDPAQ
                     SPLAFPSPRSWEYAHRALVKFADAPHLLRDALQACVGQAAGIECHAYIEHMAELPDIE
                     AILKGESAPVPQTVDLQYGVAAALVRRALQARDLPHARLVYGNILRYATRFPQREMGV
                     MLVTDMHRSVGKPLFAVPEFAAWASSITDLMLYDLKLAPNG"
     gene            948..>2021
                     /locus_tag="VFY80_02085"
     CDS             948..>2021
                     /locus_tag="VFY80_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HEX6006721.1"
                     /translation="MADPAAVVTKLGAARTRLILEKPFIGALVMHLPLVSADQRWCET
                     LATDARALYFNPDYIAALDFAQTQFVLAHEAMHCALAHFARRSHRSRRRWDIACDYAV
                     NQLLIDDGLKPPPDVLWKAEFRGLSAEEIYPLIPPDAESRTLDRHLYDDPGTSDTAQG
                     RGGSSDRKVEERQQPSMPGDSWDDAGDEGRGHAGAQSPRPLSAAERDELARKWQGRLA
                     AAAQQAHQAGRLGESWRRSIDLLLQPRLPWRQLLARYMMSTARDDYSFQRLSRREGDA
                     LLPRLASGAIDLHVVLDTSGSIGDHEIAEFIAEVDALKGQVRAKVTLHACDERLDARG
                     PWIFQAWEPLVGPERVSGGGGTSF"
BASE COUNT          335 a          672 c          657 g          357 t
ORIGIN      
        1 gatcatcgcg cagatcgcac gcgatcacgc ggtgaatctc atcgacatcc ggctctccca
       61 gatggaaccg accgatctcc gcggcgtgcc gtttcgcaaa ggcgacttcg tcgaatggtc
      121 ggtaccgtcg ctgcttccgg acgccgagcg ccacggcgcg cgcggcatcc tttttctgga
      181 cgagatcaca tccgcgccac cgacggtcac cgcagccgcc taccagctca tcctcgatcg
      241 gcgtctgggc gattaccgtg tgccgcacgg ctgggtgatc ttcgcggcag gcaatcgcta
      301 cggggaccgg ggcgtcagct acgtcatgcc gacgccgctc gccaaccgct tcacgcatta
      361 cgcgatcgag cccgacctcg aggactgggt tgcgtgggcc cacacggcag gcatcgacga
      421 gcgcatcgtc gcatttctgc gctttcgtcc cgatctcctg ttccagttcg acccggcgca
      481 gagcccgctc gctttcccga gcccgcgttc atgggagtat gcgcaccgcg cgctcgtgaa
      541 gttcgccgat gcgccccacc ttctgcgcga cgcgctgcag gcctgcgtcg gccaggcggc
      601 gggcatcgaa tgccatgcct acatcgagca tatggccgag cttccggata tcgaagcgat
      661 tctgaaaggc gagagcgcgc ccgttccgca gacggtggat cttcagtatg gcgtcgcggc
      721 tgcactggtg cggcgtgcct tgcaggcgcg cgatcttccg cacgcgcggt tggtgtacgg
      781 caatatcctg cgctatgcca cgcgctttcc gcagcgcgag atgggcgtca tgctggtgac
      841 ggacatgcac cgctcggtcg gcaagccgct tttcgcggtg ccggaattcg ccgcctgggc
      901 gagcagcatc accgatctca tgctctacga cctcaagctc gcaccgaatg gctgatccgg
      961 cggccgtcgt caccaagctg ggcgcggccc gcacccggct cattctcgag aagcctttca
     1021 tcggtgcgct cgtcatgcac ctgccgctcg tctcggcgga tcagcgttgg tgcgagacgc
     1081 tcgcgaccga cgcgcgcgca ctctatttca accccgacta catcgcggca ctcgactttg
     1141 cacagacgca gttcgtgctc gcgcatgaag cgatgcattg tgcgctcgcc cattttgcgc
     1201 gccgcagcca tcgatcgcgt cggcgctggg acatcgcctg tgactatgcg gtcaatcaat
     1261 tgctgatcga cgacggcctc aagccgccac cggacgtgct atggaaggcg gaatttcgcg
     1321 gtctctcggc cgaagaaatc tatccgctca ttccgcccga tgcggagagc agaacgctcg
     1381 accgccatct ctacgatgat cccgggacct cggacaccgc gcaaggcaga gggggatcat
     1441 cggatcggaa ggtcgaggag cgccagcagc cgtccatgcc aggcgattca tgggacgacg
     1501 ccggtgatga gggccgcggg catgcggggg cgcaatcgcc tcgaccgctg agcgccgccg
     1561 agcgggatga actcgcgcgc aagtggcagg ggcgccttgc cgcagcggca cagcaggcgc
     1621 accaggcagg cagacttggg gagtcctggc gccgatcgat cgatctgctg cttcagccgc
     1681 gactgccgtg gcggcagctg cttgcgcgct acatgatgag tacggcgcgt gacgattaca
     1741 gctttcagcg gctgtcgcgg cgggagggcg acgcactgct gccgcggctt gcaagcggcg
     1801 cgatcgacct gcacgtcgtg ctcgatacga gcggctcaat tggcgaccac gaaatcgctg
     1861 aattcatcgc cgaagtggac gccctgaaag gccaagtgcg cgcaaaggtg acgctccatg
     1921 cgtgcgacga gcggctcgat gcgcgcgggc cctggatctt tcaggcgtgg gagccgctcg
     1981 ttgggccgga acgcgtttcg ggaggcggcg ggaccagctt c
//
LOCUS       DASURG010000125         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1455674, whole genome shotgun sequence.
ACCESSION   DASURG010000125 DASURG010000000
VERSION     DASURG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455674"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VFY80_02090"
     CDS             <1..1237
                     /locus_tag="VFY80_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX6006722.1"
                     /translation="IVINSDPCIAYLMEENTMMMQALVIAHACFGHNSFFKGNYLFRQ
                     WTEADAIIDYLVFARRYVMECEERYGEAEVEAIIDSCHALSLHGVDRFKRPQPPSARE
                     ERARAEARTAYEQRQFNDLWRTVPGREPTHSTGKKRDFPAEPEENILYFVEKHSPRLE
                     PWQRELVRIVRKLAQYFYPQVQTKVMNEGWATFWHYNLINRLYDKRLINDGFMLEFLQ
                     SHTNVVCQRGFDERGFGGINPYALGFAMFTDLRRICEKPTPEDRRWFPEMAGSDWGRT
                     LDFAMRNFKDESFIGQFLSPKLMREFRFFAVADHEAESELVIDEIHDETGYRRLRKLL
                     SEQYKRENSVPDIQVVRFERDGDRSLALRHFEARNRPLKMEEAQEVMKHLSRLWGFTV
                     RLESVDPDGKVKNTLECAR"
     gene            1411..>2184
                     /locus_tag="VFY80_02095"
     CDS             1411..>2184
                     /locus_tag="VFY80_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6006723.1"
                     /translation="MQPAERFERMAQGPDEGVDVVRGALLIAAEQYPELDIEACVGVL
                     DGFAGMLRKRLRPDIGPADALRALNRYMFEELGFHGATEDYYDPRNSFLNDVIARRTG
                     IPITLAIVYMAIGRGIGLKLEGVSFPGHFLVRCPVHVGTAILDPYHRGISLGIPDLQK
                     RLAPMQGKAPTPAEVAALLAPANAREILARLLRNLRGIYMHYRQLQQALAATSRILAL
                     SPGNAHEWRERAQLHLALECFRAALTDFQSYLALAPDAED"
BASE COUNT          376 a          759 c          682 g          367 t
ORIGIN      
        1 gatcgtgatc aactccgatc cctgcatcgc atacctcatg gaagagaaca ccatgatgat
       61 gcaggcgctg gtgatcgcgc atgcctgctt cggccacaac tcgttcttca agggcaacta
      121 tctcttccgc cagtggaccg aggccgatgc gatcatcgac tacctcgtgt tcgcccgccg
      181 ctacgtcatg gagtgcgagg agcgctacgg cgaagccgag gtcgaggcga tcatcgattc
      241 gtgccatgcg ctgagcctgc acggggtcga tcgcttcaag cggccgcagc ccccttcggc
      301 gcgcgaggaa agggcgcggg ctgaagcccg cacggcctac gagcagcgcc agttcaacga
      361 cctgtggcgc acggtgcccg ggcgcgagcc cacccattcg accggcaaga agcgcgactt
      421 ccctgccgag ccggaagaga acatcctgta cttcgtcgag aagcactcgc cgcggctcga
      481 gccgtggcag cgcgagctcg tgcgcatcgt gcgcaagctc gcgcagtact tctatccgca
      541 ggtccagacc aaggtcatga acgagggctg ggccacgttc tggcactaca acctgatcaa
      601 ccggctgtac gacaagcgcc tcatcaacga cggcttcatg ctggaattcc tgcagtcgca
      661 taccaacgtc gtgtgccagc gcggcttcga cgagcgcggc ttcggcggca tcaatcccta
      721 cgcgctcggc ttcgccatgt tcacggacct gcgccggatc tgcgagaagc ccacgcccga
      781 ggatcggcgc tggttccccg agatggcggg ctcggactgg ggcaggacgc tcgacttcgc
      841 gatgcgcaac ttcaaggacg agtcgttcat cggccagttc ctctcgccga agctcatgcg
      901 cgaattccgc ttcttcgccg tcgccgacca cgaggccgaa tccgagctgg tgatcgacga
      961 gatccacgac gaaaccggct accgccgcct gcgcaagctc ctctcggagc agtacaagcg
     1021 cgagaactcg gtccccgaca tccaggtcgt gcgcttcgag cgcgacggcg accgctcgct
     1081 cgcgctgcgg catttcgaag cgcgcaaccg gccgctgaag atggaggaag cccaggaggt
     1141 gatgaagcac ctgtcccggc tgtggggctt caccgtgcgc ctagagtcgg tcgaccccga
     1201 cggcaaggtc aagaacaccc tcgagtgcgc gcgctaagcg gcgcgccgtt tgctcgcgcg
     1261 cggtccagcg tccccgcggc tcgtattcca taggcgcatt tcacagccgc cttacgtcgt
     1321 cgtttgtctc gcacggcgcg cgcatgccac aatccgccgt gtcggcatga acgtgccact
     1381 tgccccgagg gccttcgatc ggagccacgg atgcagcccg ccgagcggtt cgagcgtatg
     1441 gcgcaaggcc ccgacgaagg cgtcgacgtg gtccggggcg cactcctgat cgcggccgag
     1501 cagtatcccg agctcgacat cgaagcctgt gtcggcgtcc tggacggctt cgccgggatg
     1561 ctgcgcaagc ggttgcgccc ggatatcggg cccgccgatg cgctgcgcgc gctcaaccgc
     1621 tacatgttcg aggagctcgg ctttcacggc gcgaccgagg actactacga tccgcgcaac
     1681 agcttcctga acgacgtgat cgcgcgccgc accggcattc cgatcacgct cgccatcgta
     1741 tacatggcga tcggccgcgg gatcggcctc aagctcgaag gtgtctcgtt tccgggtcac
     1801 ttcctggtcc gctgccccgt gcacgtgggc acggccattc tcgatcccta tcaccgcggc
     1861 atttcgctcg ggattcccga cctgcagaag aggctcgcgc cgatgcaggg caaggcgccg
     1921 acgcctgccg aggtcgccgc gctgctggcg cccgcgaacg ctcgtgagat cctcgcgcgg
     1981 ctgctgcgca acctgcgcgg catctacatg cattaccgcc agctccagca ggcgctggcg
     2041 gcgacctcgc gcatcctcgc gctctcgccc ggcaatgcgc acgagtggcg cgaacgggcg
     2101 caactgcacc tggcactcga atgcttccgc gcggcgctga ccgatttcca gagctatctt
     2161 gcgctcgcac ccgatgcgga ggac
//
LOCUS       DASURG010000126         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1148732, whole genome shotgun sequence.
ACCESSION   DASURG010000126 DASURG010000000
VERSION     DASURG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148732"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1993)
                     /locus_tag="VFY80_02100"
     CDS             complement(<1..1993)
                     /locus_tag="VFY80_02100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6006724.1"
                     /translation="MDNTENNRTIAPAAGRLRQWLEASLAHRITAFSLILALFIGTVV
                     GLGSYMVLHEIVRVSIEREMSYKAALAARNVEGVINLLYRDTTRMSSNALLVRALAQT
                     PGREDPLAAAFFSAYAGPGGLSAAASVHDATGRPLAGTPGAPLRSYEGAPWLRRVLQL
                     GFTYTEIAAEGTRRYLLIARPVVSSVSAGNHGILVLRAPLDEIFGKAVASIFDDYNKR
                     LITDRGEVLAELRWRTLARPVQAAQTVPLDIAAFPAQLRLEVAGEHDEVLAPLVWLAA
                     CYAGAAGILLILLLRLARACGARLNSTLAHLAKTITAIASGPAPLPQLAVQGHDEVAR
                     IATAFNSVAATVNELQAEVESRVARRTVTLQDMNTALVKEILSHKKTGEQLHVAANAI
                     ENAAEGVMICDSEERIISINKAFTRITGYTAEEALGTTPDMLLGDDESRAKRAEISRD
                     VVDEGHWKGELWSRRKNGERYLEQCSVTAVKDEAGRLVNFIVLFSDVTKQKEDELRLQ
                     FLAHHDALTGLLNRTLFQQRCEESLMLAERKQTKAAIVFIDLDHFKTVNDSLGHAYGD
                     DLLKQAAVRLVECVRKTDVVGRLGGDEFIVLLNEVGDSGDVALIAKKILDRLTASFTV
                     AGREIFVSASLGISWFPDDGQTAATLIQNADAAMYAAKEQ"
BASE COUNT          344 a          704 c          731 g          405 t
ORIGIN      
        1 cctgctcctt cgccgcgtac atcgcggcgt ccgcgttctg gatcagcgtg gcggcggtct
       61 gtccgtcgtc tgggaaccag ctgatgccaa ggctcgcgga gacgaagatc tcgcgcccgg
      121 ccaccgtaaa cgatgcggtc agccggtcga gaatcttctt ggcgatcagc gcgacgtcgc
      181 ccgagtcgcc gacctcgttc agcagcacga tgaactcgtc gccgccaaga cgtcccacga
      241 cgtcggtctt gcgcacgcac tcgaccaggc gcacggccgc ctgcttgagg agatcgtcgc
      301 cgtaggcgtg accgagcgaa tcgttgacgg tcttgaagtg gtcgaggtcg atgaatacga
      361 tcgccgcctt ggtctgcttg cgctcggcga gcatcagcga ttcctcgcag cgctgctgga
      421 agagcgtgcg gttcagcagt ccggtcagcg cgtcgtggtg cgcgaggaac tgcagccgca
      481 actcgtcctc cttctgcttg gtgacgtcgg agaagagcac gatgaagttg acgagccgtc
      541 cggcctcgtc cttgacggcg gtgacgctgc actgctcgag atagcgctcg ccgttcttgc
      601 gccggctcca cagctcgcct ttccagtgcc cttcgtccac gacgtcgcgc gagatctcgg
      661 cgcgcttcgc acgcgattcg tcgtccccga gcagcatgtc gggggtggtg ccgagcgcct
      721 cctcggcggt gtagccggtg atccgcgtga acgccttgtt gatggagatg atgcgctcct
      781 cggagtcgca gatcatgaca ccctcggcgg cgttctcgat ggcgttcgcc gcgacgtgga
      841 gctgctcgcc ggttttcttg tggctcagga tttccttcac cagcgccgtg ttcatgtcct
      901 gcagcgtcac ggtgcgcctg gcgaccctgc tctcgacttc cgcctgcagc tcgttgacgg
      961 ttgccgctac cgagttgaag gcggtcgcga tccgcgccac ctcgtcatgt ccctggaccg
     1021 caagctgcgg cagcggggcc ggcccgctcg cgatagccgt gatggttttg gcgagatgcg
     1081 ccagcgtcga attcagacgc gcaccgcagg cgcgggcgag ccgcagcagc aggatcagca
     1141 gtatgccggc ggcgcccgcg tagcaggcgg cgagccagac gagcggggcg agcacctcgt
     1201 cgtgctcgcc cgccacctcc agccgcagtt gagccggaaa ggccgcaatg tcgagcggca
     1261 cggtctgcgc cgcttgcacc ggtcgcgcca gcgttctcca gcgcagctcg gccagcactt
     1321 cgccgcggtc ggtgatgagc cgcttgttgt aatcgtcgaa gatcgaagcg accgccttgc
     1381 cgaagatctc gtccagcggc gcccgcagca ccagtatgcc gtgattgcct gctgaaacag
     1441 acgatacaac cggacgggcg atgagcaggt agcgccgggt gccttcggct gcgatctcgg
     1501 tgtaggtgaa tcccagttgc agcacacgac gaagccacgg cgcgccttcg taggagcgca
     1561 gcggtgcgcc gggcgtgccg gccagcggcc gcccggttgc gtcgtgcacg ctcgcggctg
     1621 cggagagccc gccgggaccg gcatacgcgc tgaaaaatgc tgctgccagt gggtcctcgc
     1681 gccctggcgt ctgcgccagg gcgcgcacca gcagggcgtt ggacgacatc cgggtcgtgt
     1741 cgcgatagag caggtttatg acgccttcga cgttgcgcgc ggcgagcgcc gccttgtaag
     1801 acatctcgcg ttcgatgctg acccgcacaa tctcgtgcag caccatgtac gagcccagtc
     1861 cgacgaccgt tccgatgaaa agggcgagga tgagcgagaa ggcggtgatg cggtgtgcga
     1921 ggctcgcttc gagccactgg cgcaggcggc ctgctgcagg cgcaatcgtt cgattgttct
     1981 cggtgttatc catcccgcat ccttcttatg atggaatgct gcgaggattg cgcgcaatag
     2041 agtaacgcaa agcgccacgg ccgtggggaa attgttaccc ccgacgcgac gcttgcgtgc
     2101 tgcgcgggcg aaaggaggga cgactacttg tcctgcgcca tgtcggcctt gagaatttcg
     2161 atgccggcct gcagcgcttt ttcg
//
LOCUS       DASURG010000127         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1148786, whole genome shotgun sequence.
ACCESSION   DASURG010000127 DASURG010000000
VERSION     DASURG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148786"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="dnaX"
                     /locus_tag="VFY80_02105"
     CDS             complement(<1..1206)
                     /gene="dnaX"
                     /locus_tag="VFY80_02105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX6006725.1"
                     /translation="MTQVLARKWRPRFFSELVGQEHVVTALTNALRDQRLHHAYLFTG
                     TRGVGKTTLARIIAKALNCERGVSAEPCGTCRPCTEIDAGRHVDLIELDAASNTQVDS
                     MRELLESALYAPTSARYKVYIIDEVHMLSRSAFNAMLKTLEEPPEHVKFVLATTDPQK
                     IPVTVLSRCLQFNLKQIPQSQIRGQLQKVLEAESVAFEPAALALLARAAQGSLRDALS
                     LLDQAIAHGGGRVEEGAVRAMLGAVDHGYLYALLQALAARDGAALIAEAERMASRSLS
                     LEMALQELSVLLHRLALLQAVPAAIADDEPDRERLLELARVFDAEDLQLYYQIALQGR
                     ADIALAPDEYAGFTMTLIRMLAFAPETQRAAAGQPAALRAPAAAPRETPAAEADVSKK
                     NRDNADWPEI"
     gene            complement(1290..1874)
                     /locus_tag="VFY80_02110"
     CDS             complement(1290..1874)
                     /locus_tag="VFY80_02110"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX6006726.1"
                     /translation="MRDIQYELKSGLKNMKATAKRALYLAAAILWAASSAPSHAIDGF
                     SVEGGRGDGTDMGRVGVQWDWNKRWLQGKTWHVGGYWDLAAGYWHRSSPPGFNEDLFD
                     IGLTPVFRLQPNSLTGPYLEAAIGFHLLSRTSLGDKRFSTSFQFGDHIGAGLRFGPKG
                     AYDFGYRFQHLSNAGIKRPNNGINFHQIRFQYHF"
     gene            complement(1887..1985)
                     /gene="ffs"
                     /locus_tag="VFY80_02115"
     ncRNA           complement(1887..1985)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFY80_02115"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          367 a          674 c          687 g          418 t
ORIGIN      
        1 gatttcgggc cagtcggcgt tatcgcggtt tttttttgag acatccgctt ccgcggcagg
       61 tgtctcacgc ggtgcggcgg ctggcgcgcg caaggctgcc ggctgcccgg ctgcggcgcg
      121 ctgggtctcc ggcgcgaacg caagcatgcg aatgagcgtc atggtgaacc cggcgtactc
      181 gtccggtgcg agcgcgatat cggcgcgtcc ctgcagcgca atttgatagt agagctggag
      241 atcctccgca tcgaataccc gcgccagctc gagcagtctt tcgcgatcgg gctcatcgtc
      301 cgcgatcgcg gcaggcacag cctgcaacag ggcaagccgg tgcaggagaa cactcaactc
      361 ctgcagtgcc atctcgagcg aaaggctgcg acttgccatg cgctccgctt ccgcaatgag
      421 cgccgcgcca tcgcgtgcag cgagcgcctg gagcaatgcg tagagataac cgtggtccac
      481 cgcaccgagc attgcgcgta ccgcaccctc ctccacccga ccgccgccgt gcgcgatcgc
      541 ctggtcgagg aggctcaagg cgtcgcgcag gctgccctga gcggcgcgcg cgagcaatgc
      601 gagcgccgcc ggctcgaacg ccacgctctc cgcttcaaga actttttgca gctgcccgcg
      661 aatctgggat tggggaatct gcttcaggtt gaactgaaga cagcgcgaca ggaccgtcac
      721 cgggatcttc tggggatcgg tcgtcgcgag cacgaacttt acatgctcgg gcggctcttc
      781 gagcgtcttc agcatcgcgt taaaggcgct tcgggacagc atgtgcactt cgtcgatgat
      841 gtagaccttg tagcgggcgc tggtcggtgc gtagagcgcg ctctcgagca gctcgcgcat
      901 gctgtccacc tgcgtattgg atgcggcgtc gagctcgatc aaatcgacgt ggcgtccggc
      961 atcgatctcg gtgcacgggc ggcacgtgcc gcacggctcc gcgcttacgc cgcgctcgca
     1021 gttgagcgct ttggcaatga tgcgggcgag cgtcgtcttt cccacgcccc gcgtcccggt
     1081 gaagagatac gcatggtgca ggcgctggtc gcgcagtgcg ttggtgagcg ccgtgacgac
     1141 gtgctcctgc ccgacgagct cagagaaaaa tcggggacgc cacttacggg cgagtacttg
     1201 ggtcaatgca atgcgccgga cgagaacgtt gaggatgcgc ggcactcacg ccgcccggac
     1261 ccattttacc gtcccgaccg cgcgcgctgc tagaaatgat actggaaccg gatctgatgg
     1321 aagttgatcc cgttgttggg ccgcttgatc cccgcgttcg agagatgctg gaaacggtag
     1381 ccgaagtcgt atgcgccttt gggcccgaag cgtaggcccg cgccgatgtg gtcgccgaac
     1441 tggaagctcg tgctgaagcg cttgtcaccg agggaggttc gcgagagcag gtgaaagccg
     1501 atcgctgctt ccagataggg gccggtgagc gaattgggct gcagacgaaa caccggcgtg
     1561 agcccgatat cgaagaggtc ctcattgaag ccgggcggcg agctgcgatg ccagtatccg
     1621 gccgcgagat cccagtagcc cccgacgtgc caggtcttgc cctgaagcca gcgcttgttc
     1681 cagtcccact gcacgcccac gcgtcccatg tccgtgccat cgccccgtcc gccttcgacg
     1741 gagaacccgt cgatggcgtg ggatggcgcg ctcgatgccg cccacaggat tgcggccgcc
     1801 agatacagcg cccgctttgc cgtcgctttc atgttcttca gtccgctctt cagttcgtat
     1861 tgaatatcgc gcactttgct tgttcgggtg gcgagcccga cccccggcac ttgcagagaa
     1921 tagctgtggc tgcttccttc cggacctgac caggttcact accatgcaat gcggggaggc
     1981 ccgccatggc tgtaagtatg catgcgatcg ccggcagatg ccagcgcgca ttcaatttgc
     2041 gtctttacaa atcagttgtt taatgcttcc attggtgcca atcggaatgt caccggacgc
     2101 tcggcgcgcg atgcgccgcc cgctcgtccg tcattgctgt ttcagt
//
LOCUS       DASURG010000128         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1353267, whole genome shotgun sequence.
ACCESSION   DASURG010000128 DASURG010000000
VERSION     DASURG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353267"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFY80_02120"
     CDS             <1..322
                     /locus_tag="VFY80_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX6006727.1"
                     /translation="EQSGRNRVPEIAPVVALRDWLGQGVHNEAELRLVFAPDAERVLR
                     ALPAPRASVTLIAGPEGGLSEEEQRDAIRAGFVAVRLGPRVLRTETAALAALAAMQTL
                     WGDF"
     gene            436..1815
                     /gene="argA"
                     /locus_tag="VFY80_02125"
     CDS             436..1815
                     /gene="argA"
                     /locus_tag="VFY80_02125"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX6006728.1"
                     /translation="MSEPKNAASESVRTEVPARRLRLSAQDRFIDWFQSAAPYIHAFR
                     GKTFVIAFGGELVTDGRFAELAVDLNLLAALGVRLVLVHGARPQTEAKLKTLHHRSRF
                     HRGLRITDDVGLACAMEASGRLRVEIEAQLSTGLPNSPMAGADIRVASGNFITAKPLG
                     VLDGVDFVHTGEVRKVDTVAIQRRLQDGELVLLSPLGYSPSGEVLNLSVEDVAAATAS
                     ALRADKLIFLMDALGVTGRRGELLRELTVKRAQSLIARGALPDTDVTFYLPAAVRACE
                     QGVKRAHLISGHLDDALLLELFTHRGVGTLITPDPLETLRAARADDIGGILKLIEPLE
                     SDGTLVKRSRSLLEREMNRFIVLDHDGMVVACAALYPFTDERAGELACLAVHPDFRNQ
                     GAGERLLHEIESRARRMKLKKLFVLTTRAEHWFIERGFEEASVDVLPGERRTLYNLQR
                     RSVVLTKRL"
     gene            1887..>3117
                     /locus_tag="VFY80_02130"
     CDS             1887..>3117
                     /locus_tag="VFY80_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6006729.1"
                     /translation="MRLLIAGVLVQTVMLGLLIVNGIAVMENKLTERARSQLNDQKQF
                     LSAVLAQPLAMRDYASAQRVLDRVRREDGIVYLALYDADGNAVAASGSARETGTSGAM
                     PVRQKLEGSDPLNTEVAIEMDGRTQGWLRFGLSTAFMQSARAELVRDSLSIGIAALLL
                     SIIAMVGVSYWLTRNLVRLTRASAQMAAGDLDVHLPVETRDEVGRLTQAFNAMAAALK
                     ERVRALAESEEKFSAIADYSYDCELWINPDGKMIWVNPRVQEMFGYTPEECLATENFP
                     APFVSPKEVNRTVRQVQRALKGNTGQDYEFRARRKDGSEFWAAADWRPIFDKRGDYLG
                     IRISIRDITERRQAEQQLESTVVELREAQSVREQYLTRAQEERARLSALLAAMDMGIL
                     FVGTDDRVVYANPAFSRI"
BASE COUNT          533 a         1001 c         1043 g          540 t
ORIGIN      
        1 cgagcagagc gggcgaaacc gtgttccgga gatcgccccg gtcgtcgcgc tgcgcgattg
       61 gctgggacag ggcgtgcaca acgaggcgga gcttcgcctc gtgttcgcac ccgacgcgga
      121 gcgtgtgctc cgcgcgctgc cggcgccccg ggcgagcgtc accctgatcg cgggccccga
      181 aggcggcctc agcgaggagg agcagcgcga cgcgatccgc gcgggcttcg ttgccgtacg
      241 gctcggaccg cgcgtgctgc ggaccgaaac ggcagcgctc gctgcgctcg ccgccatgca
      301 gacgctctgg ggagacttct gatcggtgcc gtgcggctgc ggcctttcat tcgtccgggt
      361 tctcgcataa catgagcttg aaattgcgca cgaatttggc gtgagcgcgg tttcgcggct
      421 gcagggagaa cgcttatttc cgaaccgaag aacgctgcct cggagtccgt ccgaacagag
      481 gtacctgcgc gtcggctccg gctttccgcg caggatcgat tcatcgactg gtttcagtcg
      541 gccgcgccgt atatccatgc gttccgcggg aagacttttg tcattgcttt cggcggcgag
      601 ctcgtgaccg acggccgctt tgccgaactt gcggtggact tgaatctgct cgcggcgctc
      661 ggcgtgcggc tcgtcctcgt gcacggggcg cgcccacaga ccgaagccaa gctgaagacg
      721 ctgcaccacc gcagccggtt ccaccggggc ctcaggatta ccgatgacgt cgggctcgcg
      781 tgtgccatgg aagcgagcgg caggctgcgc gtggagatcg aggcccaact ttcgacgggg
      841 ctgcccaatt cccccatggc cggcgccgac atccgtgtcg cgagcggcaa cttcatcacg
      901 gccaagccgc tcggcgtgct ggatggcgtc gacttcgttc ataccggaga agtgcgcaag
      961 gtcgatacgg tggccatcca gcgccggctg caggacggcg agctcgtgct gctttcccca
     1021 ctcggctatt cgccaagcgg ggaggtgctg aatctctcgg tggaggacgt cgcggccgcc
     1081 acggcgagcg cgctcagggc ggacaagctc attttcctca tggatgcgtt gggcgtcacc
     1141 ggacgacgcg gcgagctgct gcgcgagctt accgtcaagc gggcacagtc cctgatcgca
     1201 cgcggcgcgc tgccggacac cgacgtcacg ttctacctgc cggcggcggt gcgggcgtgc
     1261 gaacaaggcg tgaagcgggc gcatctcatc tccgggcatc tcgacgatgc gctccttctc
     1321 gagcttttca cccaccgtgg cgtaggcacg ctcatcaccc cggatccgct ggaaacgctg
     1381 cgggcggcgc gcgccgacga catcggcggc atcctcaagc tgatcgagcc gctggagtcg
     1441 gacggcacgc tggtcaagcg cagccgcagt ctgctcgagc gcgaaatgaa tcgctttatc
     1501 gtgctggacc atgacggcat ggtggtcgca tgcgccgcgc tctatccgtt caccgacgag
     1561 cgcgcgggcg aactcgcctg cctcgccgtt catccggact ttcgcaatca gggggcgggc
     1621 gagcgcctgc ttcatgagat cgaaagccga gcgcgccgga tgaagctgaa gaaactgttc
     1681 gtgctcacca cgcgcgccga gcactggttc atcgagcgcg gcttcgagga agcttcagtc
     1741 gacgtgctgc cgggcgagcg gcgcacgctc tacaacctgc agcgccgctc ggtggtcctc
     1801 accaaacgcc tgtgaccccg ggcgggcaat caccaacaat ggattctgac ccggcagaca
     1861 cccgaggttt cgcaccctct ttgcgggtgc ggctactcat cgcgggcgtg ctggtgcaga
     1921 ccgtgatgct cggtctgctg atcgtgaacg gcatcgcggt catggaaaac aagttgaccg
     1981 agcgcgcccg gtcccagctc aacgatcaaa agcagtttct gagcgcggtg ctggcgcagc
     2041 cactcgcgat gcgggattac gcgagcgcgc agcgtgtcct cgaccgggta cggcgcgagg
     2101 atggcatcgt ctatctcgcg ctttacgacg cggacggaaa cgccgttgcc gcaagcggct
     2161 cggcgcgtga aaccggcacg tccggcgcca tgccggtgcg tcagaagctc gagggctcgg
     2221 atccgcttaa taccgaggtt gcgatcgaaa tggacgggcg cacgcagggc tggctgcgat
     2281 tcgggctgtc caccgcattc atgcaaagcg cgcgcgccga actcgtccgc gacagcctgt
     2341 cgatcggcat cgcggcactg ctgctttcca tcatcgccat ggtaggggtt tcctactggc
     2401 tcacgcgcaa ccttgttcgg ttgacgcgcg caagcgctca gatggcggcg ggtgatctcg
     2461 acgtgcatct ccccgtcgaa acccgcgacg aagtcgggcg gctcacgcag gcgttcaatg
     2521 ccatggcggc cgcactgaag gaacgtgtgc gcgccctcgc ggaaagcgag gagaaattca
     2581 gcgcgatcgc cgactacagc tacgactgcg agctgtggat caatcccgac ggcaaaatga
     2641 tctgggtcaa tccgcgggta caggagatgt tcggctatac gcccgaagaa tgcctcgcca
     2701 ccgagaattt tccggcaccc ttcgtgagcc ctaaagaagt gaaccgtacc gtgcgccagg
     2761 tgcagcgcgc gctgaagggc aacaccgggc aggactacga gtttcgcgcg cgtcgcaagg
     2821 atggctcgga gttctgggcc gcagccgact ggcgaccgat cttcgacaag cgcggcgact
     2881 atctcggcat ccggatcagc attcgcgaca tcactgagcg ccggcaggcg gaacagcagc
     2941 tcgagtcgac cgtcgtggag ttgcgcgagg cgcagagcgt acgcgagcaa tacctcacgc
     3001 gcgcgcagga agagcgtgcc cggctttcgg cgctgctcgc ggcgatggac atgggcatac
     3061 tctttgtcgg caccgatgat cgtgtggtgt atgcgaaccc ggccttctcg cggatct
//
LOCUS       DASURG010000129         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_433997, whole genome shotgun sequence.
ACCESSION   DASURG010000129 DASURG010000000
VERSION     DASURG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433997"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFY80_02135"
     CDS             complement(<1..454)
                     /locus_tag="VFY80_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6006730.1"
                     /translation="MAGVEVGHATIIEGEGKLVTGEGPVRTGVTAILPRGKQGHDSVF
                     AGWFALNGNGEMTGTTWITEGGILETPILITNTHSVGIARDALIAWLREHGRDFSWAL
                     PVVAETWDGMLNDINGFHVKREHVFAALNAASGGRVLQGNVGGGTGMLC"
     gene            complement(656..844)
                     /locus_tag="VFY80_02140"
     CDS             complement(656..844)
                     /locus_tag="VFY80_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006731.1"
                     /translation="MRRRHASGSTRFSEIRGLFNRVMNGQRPSGVADADEALRNDLAG
                     DRRGTAPHKISGRKGGGQ"
     gene            943..1755
                     /locus_tag="VFY80_02145"
     CDS             943..1755
                     /locus_tag="VFY80_02145"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEX6006732.1"
                     /translation="MTMASGGAAVTETIVAESWTQLQELLFAGSWNAELKRFRARGAY
                     RGSGRAGAGLHHSLSRIGGEFTQRERHIIRNFRKYAHRSFIGDESIWNWLALAQHHGL
                     PTRLLDWTYSPLIALHFVTDDLQLYQYDGEVWTVSFNETNRKLPAQLRRVLEEEGSDV
                     FTGAMLSTVAESLESFDRLSEESFVIFLEPPSVHDRIVNQAALFSIMSNPRLVLHDWL
                     AQQPDAARRIVIPAQLKWEIRDKLDQVNITERVLFPGLDGLGRWLKRYYTER"
     gene            complement(1857..>2137)
                     /locus_tag="VFY80_02150"
     CDS             complement(1857..>2137)
                     /locus_tag="VFY80_02150"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6006733.1"
                     /translation="TYYYHPHSVDLAQSLQGNLLNELGLRDIGYGRADLALVRPTWMP
                     AALTETSFMMVPEQEAALRNPDWISRIARAHVRGLEDFLRKRAAAVRR"
BASE COUNT          371 a          661 c          664 g          441 t
ORIGIN      
        1 tgcacagcat gccggtgccg ccgcccacgt tgccctgcag cacgcggccg ccgctggccg
       61 cattcagagc cgcaaacacg tgttcgcgct tgacgtgaaa gccattgata tcgttgagca
      121 tgccgtccca cgtttccgct acgacgggca gggcccagga aaaatcgcgg ccatgttcac
      181 gcagccatgc gatcagcgca tcgcgcgcga tgccgacgct gtgtgtgttg gtgatgagaa
      241 tcggtgtttc caggatgccg ccctcggtga tccaggtcgt gcccgtcatt tcgccgttgc
      301 cgttcagcgc gaaccagccg gcaaagacgc tgtcatgtcc ttgcttaccg cgcggtagaa
      361 tcgcggtcac accggtgcgc accggccctt cccccgtgac cagcttgcct tcgccttcga
      421 tgatggttgc gtgccccact tcaacgccgg ccacgtcggt gatggcgttg agaggcccgg
      481 gcgtccctgc cagcggcacg cccagatcgc gcgcggactg cgcgctcgtt ctggatgcga
      541 ggcacagcag acaaagagtg agaaggatcg ttttcatttt tcgttttagc tgcccgcaag
      601 gcgtgaccga agtctggcga ctccggtcac ggggtcaggt cacgtgtcaa tgcgttcact
      661 gtccgccgcc tttccggcca ctgatcttgt gtggggccgt gccgcggcgg tcgccggcca
      721 ggtcgttgcg caacgcctcg tcggcatcgg cgacgccgct cggacgctgg ccattcatga
      781 cgcggttgaa cagtcctcta atctcagaaa agcgggtcga gccactggcg tggcgacgtc
      841 gcatttgttt gcctcgttgt ccgtgcatga tctgcctccc ggtccgggtc tatcttccta
      901 aggtcatatt tcataccatg ttgtgagcat gacgcttgca ccatgaccat ggcttcggga
      961 ggggcggcgg tgaccgaaac gatcgtggcg gaaagctgga cccagctgca ggagctgctc
     1021 tttgcggggt cgtggaatgc ggagctcaag cgctttcgcg cgcgcggcgc atatcggggc
     1081 agcggccgcg cgggcgccgg actgcatcac agtctgagcc gcattggcgg agaattcacg
     1141 cagcgcgagc ggcacatcat ccgcaatttc cgcaaatacg cgcatcgcag ttttatcggc
     1201 gacgaatcga tctggaactg gcttgccctg gcccagcatc acgggctgcc tacccgtctg
     1261 ctcgattgga cctactcgcc gctcattgcg ctgcatttcg tgacggatga tctgcagctc
     1321 tatcagtatg atggcgaggt ctggacggtc agcttcaatg aaacgaatcg caagctgccg
     1381 gcgcagctgc gccgcgtgct ggaggaagaa ggctccgacg ttttcaccgg cgcgatgctg
     1441 tccacggtgg ccgagtcgct ggagtcgttc gatcgattgt cggaggaatc cttcgtcatc
     1501 tttctcgagc cgccttcagt gcatgaccgg atcgtgaatc aggccgcctt gttttccatc
     1561 atgtccaacc cgcgcctggt attgcacgac tggctggcgc agcagcccga cgccgcgcgg
     1621 cgcatcgtga ttcccgcgca attgaaatgg gagatccgcg acaagctcga tcaggtgaac
     1681 atcacggagc gcgtcctgtt tccgggattg gatgggctag gtcgctggct gaagcggtac
     1741 tacaccgagc ggtaatcgtg ggcgttcaag cgcgcgcgca gtttcattat aaaagcaccg
     1801 ccaagacacc agatagcgtc ccggcggtgc atttattcag ttgaactgcg tgctcatcag
     1861 cgtcggaccg ccgccgcacg cttgcgcaga aaatcttcca gtcctcgcac atgcgcgcgc
     1921 gcgatccgac tgatccaatc cggattcctg agcgcggctt cctgctcggg caccatcatg
     1981 aaactggttt cggtcagtgc ggccggcatc caggtcggac gcacgagggc caggtcggcc
     2041 cgcccgtaac cgatgtcacg cagtcccagc tcgttgagca ggttgccctg caggctttgc
     2101 gcaaggtcga cggaatgcgg atggtagtag taagtgc
//
LOCUS       DASURG010000130         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1353667, whole genome shotgun sequence.
ACCESSION   DASURG010000130 DASURG010000000
VERSION     DASURG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353667"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VFY80_02155"
     CDS             complement(<1..1566)
                     /locus_tag="VFY80_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6006734.1"
                     /translation="MDEALTALAIDARRGLSEAEAQARLEKYGKNELTAEEPVPAWRK
                     FLAQFQDVLVTLLLIATLISAGLWLYERESALPYEAMAIFAVVLLNAIMGYVQESRAE
                     QAVAALRQMSAAQANVVRDGEQKTIPAAAVVPGDVILIKEGDTIPADARMIQSTALQT
                     AEAALTGESLPVSKDTEPIPEEVGVGDRHNMIFSGTAATYGHGKAVVVATGMQTEMGR
                     IAGMLEKAPPDTTPLQKELDRVGKLLGLIVIVIAVVMIGTIILVGDVRGFAGFFDVLI
                     LGVALAVAAVPEGLPAVVTAVLALGVQRMAKRNAIVSHLAAVETLGSANVIASDKTGT
                     LTRNEMTVRAVVTASGRVTFGGTGYAPEGEVGQNGGGPIAAPLQTELARALAVGDRAN
                     NAVLQERAGHWSVKGDPTEGALIVAARKAGLTGEALDARFERVAEVPFSSQRKLMTTI
                     HKDAQSREQLRVFTKGAPDILLARCSHELIGEERRPLTAARRSEILQKNEELTGEALR
                     TLGVASRSVPAAAF"
     gene            complement(1744..>4005)
                     /locus_tag="VFY80_02160"
     CDS             complement(1744..>4005)
                     /locus_tag="VFY80_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX6006735.1"
                     /translation="GYNRMRTDIAGRIVENEDLVNFPNWLALQFRIGDEDWFDARTVK
                     ILSYRQELDLRRGMLLRTLRFEDGHGRRTTLKERRVVSMSDMHLGALELALTAENWSA
                     KVMVRSAIDGRILNAGAKLYRKFNNKHLEPLASEVVGENAVYLLVRACQSNIHVAQLA
                     RTQAFVNGELREGRRRLIDEPGYIGQELAIELEQGDTLALEKLVSCYTSRDHAVSEPA
                     VAARKAIGRAGRFGAVLADHVVAWKHLWRRFDVHIQPAGPVFKLNVPMLLRLNMFHLL
                     QAVSPNSIGLDIGVPARGWTGEAYQGHIFWDELFIFPFLNYRMPEITRSLLMYRYRRL
                     DEARAAARNAGFQGAMFPWQSGSDGQEETQELNLNPRSQRWVPDNTYLQRHVGSAVAY
                     NVWQYFQVTHDVEFLQAHGAELVLDIARFWSSIASFNAERGRYEIHGVMGPDEFHEGY
                     PNSAKPGLNNNAYTNMMAVWVLCRALDVLNLLPDIRRAELSTELALTAEEITRWQDIS
                     RRMFVPFHDGGIISQFEGYEELVELDWEGYRKRYGNIQRLELILEAENDSANRYKLSK
                     QADVLMVFYLFSAEELGELFTRLGYPFKPEMIPRNIAYYDARSSHGSTLSRVVHAWVL
                     ARSDRPRSSRYFAEALQSDVSDIQQGTTAEGVHLGAMAGTVDLVQRVSTGIEITGDVL
                     RLNPRLPEDMERLDMRIRYRGHTLDLRLTRDTLTVRGREHGAAPINLGFKGAVYEFAG
                     GSTRVFKLESKPR"
BASE COUNT          745 a         1316 c         1171 g          773 t
ORIGIN      
        1 gaatgctgcc gccggcacgg atcgggaagc gacaccgagt gtgcgcaacg cttcgccagt
       61 gagctcttca tttttttgca aaatctccga gcggcgggcc gccgtcagag gccggcgctc
      121 ctctccgatc agctcgtgcg aacaccgcgc aagcaggata tccggtgcgc ccttggtgaa
      181 tacccgcaac tgctcccggc tttgcgcatc cttatggatc gtcgtcatca atttgcgctg
      241 ggaggagaaa ggcacctcgg cgacgcgctc gaagcgcgcg tcgagcgctt cacctgtcag
      301 gccggctttg cgcgcagcga cgatcagcgc gccttcggtc ggatcgccct tcactgacca
      361 atggccagcg cgctcttgca ggacggcatt gttcgcccgg tcgcccaccg cgagcgcgcg
      421 cgccaactcg gtctgcaaag gagcggcgat cggtccgcct ccgttttggc cgacttcgcc
      481 ttcgggagca taaccggtac ccccgaatgt cacgcgtcca ctcgcggtga ccaccgcgcg
      541 caccgtcatc tcgttcctag tgagtgtccc ggtcttgtcc gaagcgatca cgttcgcaga
      601 accaagagtc tccaccgccg cgaggtggct cacaatggcg ttgcgcttcg ccatccgctg
      661 cacacccagg gcaagtacgg cggtcaccac cgcgggcaat ccctcgggca cggcagccac
      721 cgcaagcgcc acaccgagaa tcaaaacgtc gaaaaaaccg gcaaacccgc gcacgtcgcc
      781 gacgagaatg atcgttccga tcatcaccac cgcgatgacg atgacaatca atcccagcag
      841 cttgccgaca cggtcaagct ccttctgcaa cggtgtggta tccggcggcg ccttttccag
      901 catcccggca atacgcccca tctccgtttg cattccggtc gcgacaacca ccgctttgcc
      961 atgcccgtag gtcgccgccg tgccgctgaa gatcatgttg tggcggtcgc ctactccaac
     1021 ctcttcggga atgggctcgg tgtccttcga taccggcagg ctctcgcccg tcaacgccgc
     1081 ctccgccgtc tgcagcgcgg ttgactggat catccgcgcg tcggctggaa tggtgtcgcc
     1141 ttcttttata aggatgacgt ctccgggaac gactgcggcc gcggggatgg tcttctgctc
     1201 accatcgcgc acgacattgg cctgcgcagc cgacatctgc cgtagcgccg cgaccgcttg
     1261 ttccgcgcgt gactcctgga cgtagcccat gatcgcgttg agcagaacga cggcgaaaat
     1321 ggccatcgct tcgtaaggca aggccgattc gcgctcgtac agccatagtc ccgccgagat
     1381 caatgtggcg atcagcagca gggtgacgag cacgtcctga aactgcgcga ggaacttcct
     1441 ccacgccggc acgggctctt ccgccgtcag ctcgtttttg ccatattttt cgagccgtgc
     1501 ctgcgcctcc gcctcgctca acccacgccg cgcgtcgata gcgagcgcag tcaatgcctc
     1561 gtccacggat tgctggtagg gagcactact cgcatcccta gaacgtttcg cggagctcgt
     1621 agattgttcc gtcacagtgc tcatcgatac ggtctcacca cgaagcgccg gatgttcgga
     1681 tgatgcgcct ttctaacctt cgtgcgcttc gtgggtgaac atctttttcc agcgcattac
     1741 ggatcatcgc ggtttactct cgagcttgaa aacacgcgtg ctgccgccgg cgaactcgta
     1801 caccgcgccc ttgaagccga ggttgatcgg cgcagcgccg tgttcgcgcc cacgcaccgt
     1861 aagcgtgtcg cgcgtcaggc gcagatccaa cgtatgcccg cgatagcgga tgcgcatgtc
     1921 gagacgctcc atgtcttccg gcaaacgtgg attgagccgc agcacgtccc cggttatttc
     1981 gatccccgtc gacacgcgct gcaccagatc gacggttccg gccatggcgc cgagatgcac
     2041 tccctcggcg gtggtgccct gctggatatc gctcacgtcg ctttgcagcg cctccgcgaa
     2101 atagcgtgac gatcgcggcc ggtccgagcg cgccaatacc cacgcatgga ccacacggga
     2161 gagcgtggag ccgtgcgagg agcgggcgtc gtagtaagcg atgttgcgcg gaatcatctc
     2221 gggtttgaac ggataaccca ggcgcgtgaa cagctcgcca agctcctctg cggaaaacag
     2281 gtaaaacacc atcagcacgt cagcctgttt cgacagtttg tagcggttcg cgctgtcgtt
     2341 ctccgcctcg aggatgagct cgaggcgctg gatgttgccg taccgcttcc gatagccctc
     2401 ccaatccaac tcgacgagtt cctcgtagcc ttcgaactgg ctgatgatgc cgccgtcatg
     2461 aaacggcacg aacatccggc gactgatatc ctgccagcgc gtgatctctt ccgccgtcaa
     2521 agcgagctcg gtgctgagct cggcgcgccg aatgtccgga agcagattga gcacatccag
     2581 ggcccggcac agcacccaga ctgccatcat gttggtgtag gcgttgttgt tgagaccggg
     2641 ctttgccgaa ttcggataac cctcgtgaaa ctcgtcggga cccatgacgc cgtgaatctc
     2701 atagcggccg cgctccgcgt tgaaactcgc gatgctcgac cagaaccggg cgatatcgag
     2761 caccagctcg gcgccgtggg cctgcagaaa ctcgacatcg tgcgtcactt gaaagtattg
     2821 ccagacgtta taggcgacgg cgctgccgac gtggcgttgc aggtaggtat tgtcaggcac
     2881 ccagcgttga gagcgcgggt tcaggttcag ctcctgggtt tcctcttgtc cgtcgctgcc
     2941 gctttgccag ggaaacatcg ccccctgaaa tcccgcgttt ctggcggcgg cgcgcgcctc
     3001 gtcgaggcgg cggtagcgat acatgagcag ggacctggtg atttcgggca tgcgataatt
     3061 gagaaacggg aagataaaaa gctcatccca gaaaatgtgc ccctggtagg cctcccccgt
     3121 ccagccgcgc gcaggcacgc cgatatcgag gccgatggag ttgggcgaca ccgcttgcag
     3181 cagatggaac atgttcaacc gcagcagcat cggaacgttc aacttgaaca cggggccggc
     3241 cggctggatg tgcacgtcga agcggcgcca caagtgcttc caggcaacca catgatccgc
     3301 cagcaccgcg ccgaagcgac ccgcgcgccc aatcgctttg cgcgccgcca cggccggctc
     3361 ggaaacggcg tgatcgcgcg acgtgtaaca agacacgagc ttctccagcg ccaaagtgtc
     3421 gccttgctca agctcgatcg ccagttcctg cccgatgtag cccggttcat cgatcagccg
     3481 gcgccggcct tcgcgcaact cgccattcac gaatgcctgc gttcgcgcca gctgcgccac
     3541 gtgaatattc gactggcagg cgcgcaccag cagatagacg gcgttttcgc caaccacttc
     3601 gctcgccagt ggctccaggt gtttattgtt gaatttgcga tagagcttcg cgcctgcatt
     3661 gagaatacgg ccgtcgatcg ctgaacgcac catgaccttt gcggaccagt tctccgcggt
     3721 gagcgccagc tcgagcgcgc ccaggtgcat gtcgctcatc gacacgacgc ggcgttcctt
     3781 gagcgtcgtg cgccgcccgt ggccgtcctc gaaccttagg gttcgcagca gcataccgcg
     3841 ccgcagatcg agctcctggc gataggacag gatcttcacg gtcctggcat cgaaccaatc
     3901 ctcatcgccg atgcgaaact gcagcgcgag ccaattggga aaattgacca gatcttcgtt
     3961 ctcgactatt cggccggcaa tatcggtgcg catgcggttg tagcc
//
LOCUS       DASURG010000131         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_434432, whole genome shotgun sequence.
ACCESSION   DASURG010000131 DASURG010000000
VERSION     DASURG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434432"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2163
                     /locus_tag="VFY80_02165"
     CDS             <1..>2163
                     /locus_tag="VFY80_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX6006736.1"
                     /translation="AANVYTVKRHGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCM
                     SFYDWYCDLPPSSPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRY
                     KGAKTVAVTPDYAEVAKLCDLWLHPKQGTDAALALAMGHVILKEYHLSGKSAYFEEYC
                     RRYSDMPMLVRLVKQGERYVPDRLLRASDFADQLGETNNPDWKTVALDEASGAFVAPR
                     GSIGFRWGEKGKWNLEEKAGQAGVDARLSLSLVGRHDEVVKAAFPYFGGREHAHFESS
                     AHDEVLERNIAAKRVKLKDGDGLVATVFDLFVANYGLDRGLGGPNVAKSYDDDVPYTP
                     AWQEKITGVERELVIRVAREFADNADKTHGKSMVIIGAAMNHWYHMDMNYRSIINMLV
                     MCGCVGQSGGGWAHYVGQEKLRPQTGWTALAFALDWARPPRQMNSTSFFYAHTDQWRY
                     EQLKVSEILSPLAPAGKYTGTLIDFNVRSERMGWLPSAPQLAANSLQIARDAAAAGMD
                     PKDYAVQQLKSGALKLACEDPDNPTNFPRNMFVWRSNLLGSSGKGHEYFLKHLLGTQH
                     GVQGKDLGEMGAEKPEEVVWHDAAPEGKLDLLVTIDFRMSTTCLYSDVVLPTATWYEK
                     NDLNTSDMHPFIHPLSAAIDPVWQAKSDWEIYKGFAKKFSELVPGHLGVEKDLVLTPT
                     QHDTPGELAQALDVKDWKKGECAPLPGRTMPAMTVVER"
BASE COUNT          419 a          688 c          694 g          362 t
ORIGIN      
        1 cgcggcgaac gtctacacgg tcaaacgaca cggccccgac cgggtgatcg gcttctctcc
       61 gattcctgcg atgtcgatgg tgtcctacgc cgccggcagc cgctacctct cgctcatcgg
      121 cggcgtgtgc atgagcttct acgactggta ctgcgatctt ccgccgtcga gcccgcaaac
      181 ctggggcgag cagaccgacg tgccggagtc cgccgactgg tacaactcga gctacatcat
      241 cgcatggggc tcgaacgtgc cgcagacacg cacgcctgac gcgcatttct tcaccgaagt
      301 gcgctacaag ggcgccaaaa ccgtcgccgt cacgcccgat tacgccgagg ttgccaagct
      361 ctgcgacctg tggctgcatc ccaagcaggg aacggacgca gcgctcgcgc tcgcaatggg
      421 gcacgtgatc ctgaaggagt atcacctctc cggcaagagc gcgtacttcg aggagtactg
      481 ccgccgctat tccgacatgc cgatgctcgt gcgcctggtg aagcagggcg aacgctacgt
      541 ccccgaccgc ctgctgcgcg cctccgattt cgcggatcag ttgggcgaga ccaacaaccc
      601 cgattggaaa accgtggcgc tggacgaagc gagcggtgcg ttcgtcgccc cgcggggctc
      661 gatcggattc cggtggggcg agaagggcaa gtggaacctc gaggagaagg cgggccaggc
      721 cggtgtcgat gcgcggctca gtctctccct cgtcggccgg cacgacgaag tcgtcaaagc
      781 ggcattcccg tatttcggcg gacgcgagca cgcgcatttc gaatcgagcg cgcacgacga
      841 agtgctggag cgcaacatcg cggcgaagcg cgtaaaactc aaggacggcg atgggctcgt
      901 cgcgacggtc ttcgatctct tcgtcgcgaa ctacgggctc gatcgcgggc ttggcgggcc
      961 caacgtcgcg aagtcgtacg acgacgacgt gccctatacc cccgcatggc aggagaagat
     1021 cacgggcgtg gagcgcgaac tggtgatccg tgtcgcgcgc gaattcgccg acaacgccga
     1081 caagacgcat ggcaagtcga tggtgatcat cggcgcggcg atgaatcact ggtatcacat
     1141 ggacatgaac taccgttcca tcatcaacat gctggtgatg tgcgggtgcg tggggcaatc
     1201 gggcggcggc tgggcgcatt acgtggggca ggagaaactg cgtccgcaaa ccggctggac
     1261 ggcccttgcg ttcgcgctcg actgggcacg tccgccgcgc cagatgaatt cgacctcgtt
     1321 cttttacgcc cataccgacc agtggcgcta cgagcagctc aaggtctccg aaatcctttc
     1381 gccgctcgcc ccggccggca aatacacggg gacactcatt gatttcaacg tgcgctccga
     1441 gcgcatgggg tggctccctt cggcgcccca gctcgcggcg aattcgctac agatcgcgcg
     1501 cgatgcggcc gcagccggca tggatccgaa agactacgcc gtgcagcagc tcaagtccgg
     1561 tgcgctcaag ctcgcctgcg aggacccgga caaccccacc aatttcccgc gcaacatgtt
     1621 cgtatggcgc tcgaatctcc tcggctcctc gggcaagggg cacgaatact ttctgaagca
     1681 cctgctcggc acgcagcacg gcgtgcaggg caaggacctg ggcgagatgg gggcggagaa
     1741 gcccgaggaa gtggtgtggc atgacgccgc gccggaaggc aagctcgatc tgctcgtcac
     1801 catcgacttt cggatgtcca cgacctgcct ctattcggac gtcgtcctgc cgactgccac
     1861 ctggtacgag aagaacgatc tcaatacgtc cgacatgcac ccgttcatcc acccgctgtc
     1921 ggccgcgatc gatccggtat ggcaggcgaa gagcgactgg gaaatctaca agggctttgc
     1981 gaagaagttc tctgagctcg tgcccgggca cctgggcgtc gagaaggacc tcgtgctgac
     2041 gcccactcag cacgacacac cgggcgaact cgcgcaggcg ctcgatgtga aggactggaa
     2101 gaaaggcgaa tgtgcgccgc tgccgggcag gacgatgccg gcgatgacgg tggtcgagcg
     2161 tga
//
LOCUS       DASURG010000132         4725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_26677, whole genome shotgun sequence.
ACCESSION   DASURG010000132 DASURG010000000
VERSION     DASURG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26677"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFY80_02170"
     CDS             <1..966
                     /locus_tag="VFY80_02170"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX6006737.1"
                     /translation="NMMETVRTIEVRPTAAPLGAEIRCGDVCMLTEEAVQAIRRAWLE
                     HLVVVFRHQTITNDDLVAFGHRFGEFQYSNPLPSPLAHEGKVKQGGRDEAHPEITVVS
                     NIVEKGVAIGGFGDAELVWHTDMSSFEVPPNQTVLYALEVPQSGGSTGFANMYAAFDT
                     LPVELKARARGLRLKHDATTDAAGYLRKQYADTRDMDLRASPGAVHPLVVTHPETGCE
                     CLYLGRRSRAYIDGLAVDESEALLDALWAHATQPRFAWRHQWQVGDLVMWDNRCVMHH
                     REPFDGAQHRRLHRVVIKGSKPFHRSGEVPAPHARARLQAAQPVY"
     gene            999..1928
                     /locus_tag="VFY80_02175"
     CDS             999..1928
                     /locus_tag="VFY80_02175"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6006738.1"
                     /translation="MANMKQHKLGWIGIGRMGYAMAERLAKAGCDLSVWNRTRAKAEP
                     LAAHGAKIVDSLSELAGCDIVFTMVAAGKDVKEVLFGPNGVTSRGKAPKIVVDSTSIS
                     LEESAEVREKLGKLNVKLLAAPVSGNDKVVKAGKLMFVVSGPRDAYDAAQAYLDAMGR
                     GSSYVGDGELSRIAKICHNVHLSIVIQSLCEITLLAQKAGMPRHAFLEFINSSIFGSM
                     FTRYKTPALVNLDFHVTFTPELLRKDIDLGLSEGRKFGVPMPLASITRDLVQTLIGHG
                     HTDKDFAALLLLQAQASGMDLKPENVQVSDGLS"
     gene            1953..2924
                     /locus_tag="VFY80_02180"
     CDS             1953..2924
                     /locus_tag="VFY80_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006739.1"
                     /translation="MKTVIGPFAAALLVAYALSGHAAAPSFPTKPVRVIIAFPAGSAT
                     DIIGRVITHKVGEYWGQTVIADNRGGAGGSIASSIAAKAAPDGYTLIINSSAHAINPS
                     MYAKLPYDTRKDFTDITRLVGQPNVLVVQPAASFKSLGDFLAEARAKPGTINFAFAGL
                     GSGTHLNLEKFKLAAKVDVTPVSYKGSGEAINDVMGGRVHTYFAPISAGISFIRSNRV
                     RALAVTSAQRSGQLPDVPTVAESGVPGFEFILWFGLWGPAGMPQPILDRVWTDFTRAL
                     ADPATREKLATLGNEAMPMKPREFTKFVNQELAEYARIFKAAGIKPQ"
     gene            2956..3753
                     /locus_tag="VFY80_02185"
     CDS             2956..3753
                     /locus_tag="VFY80_02185"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6006740.1"
                     /translation="MNDTVTVAAPRIAMAKAAKRFGTLEVFRDVSLEVGEREVLAIIG
                     PSGCGKTTLLRCIDGLLPLTEGEIRVEGERVTAPRDGVAMVFQHFGLFPWKTVFQNVA
                     YGLKLAGCPGAEIARKVPKYISLVGLSGFEHYYPYQISGGMQQRCGLARALSVEPRVL
                     LMDEPFGAVDAQTREILQFEMLRIWEMRPTTMVFVTHAIDEAVLMGDRVLVLKGRPSS
                     VHEIIDVDLPRPRDRSTLALPRFAQLRERVWGTLMDDAKRAEFDVRR"
     gene            3788..>4725
                     /locus_tag="VFY80_02190"
     CDS             3788..>4725
                     /locus_tag="VFY80_02190"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6006741.1"
                     /translation="MTVSRTLRAVGAVVGVALGLALFPASGVAQQKYTFGIPGIPPVF
                     GGTIALVAEKEGFFKKRGVDVTVRAFETGAAASRAVASGEIAVALSPTPLIVNQISNT
                     DVKLVGIWGMEHPDWLIGATDPNADCASMKGQAVGVDSLGGARSIALRTMLVGCKMKI
                     EEVQQVPLSSNVGTAMVAGQLKFGVLHIDDIPVIEAEMKKPLKTVITQKDVRPVDHYL
                     LLVANQDQLAKNRDAYVRMVAALIDAERFMRNPANVEKVAKYAEPTGRTGKIAAQALK
                     AYVEMEFWPKDKDGLDAKKLEMVAAIQKKVGNLKAE"
BASE COUNT          893 a         1435 c         1571 g          826 t
ORIGIN      
        1 aatatgatgg aaacggtgcg gacgatcgaa gttcgaccga cggcggcgcc gctcggggca
       61 gagatccgct gcggcgacgt atgcatgctc accgaagagg cggtgcaagc gatccgccgc
      121 gcgtggctcg agcatctcgt ggtggtgttt cgccaccaga caatcacgaa cgacgacctc
      181 gtggcgttcg gccaccgctt cggcgaattc cagtactcga atccgcttcc gagcccgctc
      241 gcgcacgagg gtaaagtgaa acagggtggc cgggacgagg cccatcccga aatcaccgtc
      301 gtgtcgaaca tcgttgagaa aggcgttgcc atcggcggct ttggcgacgc agagctcgtg
      361 tggcataccg acatgtcgag cttcgaggtg ccgcccaatc agaccgtgct ctacgcgctc
      421 gaagtgccgc agagcggcgg ctccaccggt tttgcgaaca tgtatgccgc gttcgatacg
      481 ttgcccgtag aactgaaggc acgcgcgcgg gggctgcgcc tcaagcacga cgccacaacc
      541 gatgcggcgg gctaccttcg caagcagtac gccgatacgc gcgacatgga tcttcgagcg
      601 agtccgggcg cggtgcatcc gctcgtcgtg acgcatcccg aaacgggatg cgaatgcctc
      661 tatctggggc gccgttcgcg cgcctatata gacgggctcg ccgtggatga atcggaggca
      721 ttgctcgatg ccctctgggc gcatgcgact cagccgcggt tcgcatggcg ccatcagtgg
      781 caggtgggcg acctcgtcat gtgggacaac cgctgcgtca tgcaccatcg tgaaccgttc
      841 gacggcgcgc agcaccgccg gctgcaccgc gtcgtgatca agggcagcaa gccgttccat
      901 cgttcgggcg aggtgcccgc gccgcacgcg cgcgcccggc tacaggcggc ccaaccggta
      961 tactgaatcg acaacctctt cacgcgacag gacacgcaat ggcaaacatg aagcagcaca
     1021 agctgggatg gatcggcatc gggcgcatgg gctacgcgat ggcggaaagg ctcgccaagg
     1081 caggatgcga cctttcggtg tggaaccgca cgcgagcgaa agcggagccg ctcgccgcgc
     1141 acggtgcgaa gatcgtggac tcgctctccg aacttgcggg gtgcgacatc gtgttcacca
     1201 tggtcgcggc agggaaggac gtgaaggagg tgctgttcgg gccgaacggc gtaacttcca
     1261 ggggcaaggc gccgaagatc gtcgtggaca gcacttcgat ctcccttgaa gagtcggccg
     1321 aagtgcgcga gaagctcgga aagctcaatg tcaagctgct tgccgcgccc gtgtccggaa
     1381 acgacaaggt ggtgaaggcc ggcaaattga tgttcgtcgt atccggcccg cgcgacgctt
     1441 acgatgccgc acaggcgtac ctcgatgcca tgggccgcgg gtcgagctat gtcggggatg
     1501 gcgagctctc gcgcatcgcc aagatctgcc acaacgtcca tctgagcatc gtgatccagt
     1561 cgctctgtga aatcacgctg ctcgcgcaaa aggccggaat gccgcggcac gcgttcctgg
     1621 agttcatcaa cagcagcata ttcggcagca tgtttacgcg ctacaagact cctgccctgg
     1681 tgaacctcga cttccatgtc acgtttacgc cggagctgct gcgcaaggat atcgatctcg
     1741 gactttccga gggcaggaag ttcggtgtgc ccatgccgct tgcctcgatc acgcgagacc
     1801 tcgtgcagac gctcatcggg cacggacata cggacaagga ctttgccgcg ttgctgctgc
     1861 tgcaggccca ggcttcagga atggacctga agcccgaaaa cgtgcaggta agcgacggcc
     1921 tgtcctgatc gccgccaatg ccggagggga gaatgaaaac agtcatcggg ccgttcgcgg
     1981 ccgcattgct cgtggcctac gcgctcagcg ggcacgcggc ggcaccatcg tttcccacga
     2041 agccggtacg cgtcatcatc gcgtttccgg cgggaagcgc caccgatatc atcggccgcg
     2101 tcatcacgca caaggtgggc gagtactggg gccagacggt aatcgcggac aatcgcggcg
     2161 gcgcgggcgg gtcgatcgcc tcctcgatcg cggcgaaggc agcccccgac ggctacacgc
     2221 tcatcatcaa ctcctcggcg cacgcgatca atccctcgat gtacgccaaa ctgccctacg
     2281 atacgcgcaa ggatttcacc gacatcacgc ggctcgtggg tcagccgaat gtcctcgtgg
     2341 tgcagccggc cgcgtcgttc aagtcgctcg gcgattttct ggccgaggcg cgcgccaagc
     2401 cgggcaccat caacttcgcg tttgccgggc tgggcagcgg cactcacctc aacctggaga
     2461 agttcaagct ggccgccaaa gtggacgtga cgccggtctc gtacaaaggt tccggtgaag
     2521 cgatcaacga tgtgatgggc gggcgcgtcc atacctactt cgcgccgatt tctgccggta
     2581 tctcgttcat ccgctcgaac cgggtgcgcg cgctcgctgt gacttcggca cagcgctcgg
     2641 gtcagttgcc cgacgtgccg accgtggccg agagcggtgt gccgggattc gagttcatcc
     2701 tgtggttcgg gctgtggggt ccggcgggca tgccgcagcc gattctcgac agggtctgga
     2761 ccgatttcac gcgcgcgctt gcggatcccg ccacgcgcga gaagctcgcg acgctgggca
     2821 acgaagcgat gccgatgaag ccgcgcgagt tcacgaagtt cgtgaatcag gaactcgcgg
     2881 agtacgcccg catcttcaag gcggccggca tcaagccgca gtgacgcgac gtgcagtccc
     2941 tcgcggagtg agaaattgaa cgatacggta acagtcgctg caccgcgcat tgcgatggcg
     3001 aaggcggcga agcgcttcgg cacgctcgaa gtgtttcgcg acgtcagcct ggaggtgggg
     3061 gaacgcgagg tgctcgccat catcggccct tcgggttgcg ggaagacgac gctgctgcgc
     3121 tgcatagacg gcctgctgcc gctcacggag ggtgaaattc gcgtcgaggg cgagcgcgtc
     3181 accgcgccgc gcgacggtgt cgcaatggtg ttccagcact tcggtctctt tccctggaaa
     3241 acggtgttcc agaacgtcgc gtacgggctg aagcttgccg gctgcccggg cgcggagatc
     3301 gcccgtaaag tgccgaagta catcagcctc gtcggcctgt ccggattcga gcactactat
     3361 ccgtaccaga tttcgggcgg gatgcagcag cgctgcggcc tcgcgcgcgc gctttcggtg
     3421 gagccgcgcg tgctcctgat ggacgagccg ttcggtgcag tggatgccca gacgcgcgag
     3481 atcctgcagt tcgagatgct gcgcatctgg gagatgcggc ccaccaccat ggtgtttgtc
     3541 acgcatgcga tcgacgaggc ggtgctcatg ggcgatcgcg tgctggtgct gaaggggcgc
     3601 ccgagcagcg tgcacgagat catcgacgtc gatctcccgc gcccgcggga tcgctcgact
     3661 cttgcgcttc cgcggttcgc acagttgcgc gagcgcgtgt gggggacgct gatggatgac
     3721 gcgaagcgcg cggaattcga tgtcaggcgt tagtttgcag agtgcggttc aaccaggagg
     3781 aacgatcatg acagtcagtc gcacgttacg ggcagtcggt gctgtggtgg gtgttgcgct
     3841 ggggcttgcg ctctttccgg catccggtgt ggcgcagcag aaatacacgt tcggcatccc
     3901 cggcattccg ccggtcttcg gaggaacgat cgcgctcgtc gcggagaagg aaggcttctt
     3961 caagaagcgt ggcgtggacg tgacggtgcg cgccttcgag acgggcgccg cggcttcccg
     4021 cgcagtcgct tcgggagaga ttgcggtggc gctctcaccc acgccgctta tcgtgaacca
     4081 gatctcgaat accgacgtga agctcgtcgg catctgggga atggagcatc ccgactggct
     4141 gatcggcgcg accgatccga atgccgactg cgccagcatg aaaggccagg ccgtcggcgt
     4201 cgattctctg ggcggcgcgc gctcgatcgc gctgaggacc atgctcgtcg gctgcaagat
     4261 gaagatcgag gaagtccagc aggtgccgtt gagctcgaac gtcggcaccg ccatggtcgc
     4321 aggccagctc aagttcggcg tcctgcacat cgacgacatt cccgtcatcg aagcggaaat
     4381 gaaaaagccg ctcaagacgg tgatcacgca gaaggacgtc cggccggtgg atcattatct
     4441 gctgctcgtc gcgaaccagg atcagcttgc aaaaaaccgc gatgcctatg tgcgcatggt
     4501 cgcggcgttg atcgacgccg agcgcttcat gcgcaatccg gcgaatgtcg agaaggtcgc
     4561 gaaatacgct gaaccgaccg gccgcaccgg gaagatcgcg gcgcaggcgc tgaaggccta
     4621 cgtggagatg gaattctggc ccaaggacaa ggacggcctc gatgcgaaga aactcgagat
     4681 ggtggctgcc atccagaaga aggtcggcaa cctcaaggct gagaa
//
LOCUS       DASURG010000133         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1251740, whole genome shotgun sequence.
ACCESSION   DASURG010000133 DASURG010000000
VERSION     DASURG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251740"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFY80_02195"
     CDS             <1..733
                     /locus_tag="VFY80_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6006742.1"
                     /translation="ACKARGIRVLTLRRRANIACAEHAFALMLAAARRICATNGRLSM
                     KGLEAAGYSPTAYDRAHTANSNWARVTGLRTLYGAQLGIVGLGEIGREIALRAIAYGM
                     RTVYTQRHRLDSEDEARYGATYTTLDALLAASDFVSLNLPGNAGTRGIIGPQELGRIK
                     DDAILVNVSRADLVDRTALVEALRMGRLGGYVTDLPYEEPGHDDDPLLQFRNVIITPH
                     IAAQPRFNALDDFEELLVNLDRALS"
     gene            743..1771
                     /locus_tag="VFY80_02200"
     CDS             743..1771
                     /locus_tag="VFY80_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006743.1"
                     /translation="MTLAKDFSVLKTRTVGSRAVRCMQALVFLALTAAHACALSAQPA
                     FPARPIRFIVPFPPGTSTDVVVRLVSQRLADALGQQIVIDNRAGASGMLGVEIGARAA
                     PNGYTWVLGTTSTHALAPTLNPRLAYDPVKDFAPVTMLGDAPYFMTTHPGIPATSVKA
                     FIAYVRANAGKVHYASVGNASLGHLSGELFKKAAGIDMVHVPYKGSTLAMVDLIAGRI
                     QLQITTVPASLPQVKAGKLRALAVTSRERISVLPQLPTVAESGLPGYYAALWMTVFVP
                     AGTPPPVIGRINRELSAAMGADAMRDALTDQGFVPRPLGPDDAARLLREETARWRKVI
                     VDAGIKSE"
     gene            complement(1793..>2288)
                     /locus_tag="VFY80_02205"
     CDS             complement(1793..>2288)
                     /locus_tag="VFY80_02205"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6006744.1"
                     /translation="SLLFVAGTLAATGDMYIHGLFALLVLASFSGDNTNYWIGRFVGP
                     RVFSQQQSWALNPAHLERTRRFYERHGGKTIVIARFIPIVRTFAPFVAGIGRMPYPRF
                     MFFSFAGSVFWIGSLTYAGYFFGNLPVVKDNLSLVIIGIVILSIMPGVVEYLRSRARR
                     RIAA"
BASE COUNT          412 a          761 c          732 g          383 t
ORIGIN      
        1 cgcgtgcaag gcgcgcggca ttcgggtgct gacactgcgc cggcgtgcca atatcgcttg
       61 cgccgagcac gcgttcgcgc tgatgctcgc tgccgcgcgc cggatctgcg cgacgaacgg
      121 acgcctcagc atgaaaggcc tggaagcggc cggctactca ccgaccgcat acgaccgcgc
      181 gcacaccgcc aattccaatt gggcccgggt gacgggattg cgaacgttgt acggcgcaca
      241 gcttggcatc gtcggcctgg gagaaatcgg ccgcgagatc gcgcttcgtg cgatcgcgta
      301 cggcatgcgc accgtctaca ctcagcgcca tcgtctcgac tcggaggacg aagcgcgtta
      361 tggggcgacc tatacgacgc tcgacgcact gctcgcagcg agcgacttcg tgagcctcaa
      421 tctgccgggc aacgccggca cccgcggcat catcgggccg caagaacttg gcaggatcaa
      481 ggacgatgcc atcctggtaa atgtgtcgcg cgcggatctc gtcgatcgca ctgcgctcgt
      541 cgaagcactg cgcatgggac gcctcggcgg ctacgtcacc gatctgccct acgaggagcc
      601 cgggcatgac gacgatccgc tgctccaatt ccgtaatgtc atcatcacgc cccacatcgc
      661 ggcgcagccg cgcttcaacg ccctcgacga tttcgaggag ctgctcgtga acctcgaccg
      721 ggcgctcagc tgaggggtca ccatgacgct cgcaaaagat ttttccgtcc tgaagacacg
      781 cactgtgggt tcgcgtgcgg ttcgctgcat gcaggcgctc gtattcctcg cgcttaccgc
      841 cgcgcatgcg tgcgcgttgt cggcgcaacc cgccttcccg gcccgaccga tccgtttcat
      901 cgtgccgttt ccgccaggta cttccaccga tgtcgtggtg cgcctcgtct cgcaacggct
      961 cgccgacgcg ctggggcagc agatcgttat cgacaatcgc gcgggtgcga gcggcatgct
     1021 cggcgtggag attggcgcgc gggcggcgcc gaacggctac acctgggtgc tcggcacgac
     1081 cagcacccac gcgctcgcac ctactctcaa tccgcggctc gcctacgatc cggtgaagga
     1141 cttcgccccg gtcaccatgc tcggtgacgc gccctatttc atgacgacgc atccgggaat
     1201 accggcgacg agcgtcaagg cattcatcgc ctacgttcgc gcgaacgccg gcaaggtgca
     1261 ctacgcttca gtgggcaacg cgagcctggg gcacctctcc ggcgagcttt tcaagaaggc
     1321 cgcgggcatc gacatggtgc acgtgcccta caagggctcc acgctcgcga tggtcgatct
     1381 gatcgcgggc cgcattcagt tgcagatcac gaccgttccc gcgtcgctgc cgcaggtgaa
     1441 ggcggggaag ttgcgtgcgc tcgccgtgac gagccgcgaa cggatttccg tgttgccgca
     1501 gttgcccacg gtcgcggagt ccggcctgcc cggctattac gcggcgctct ggatgacggt
     1561 gttcgtcccc gcgggcacgc cgccgccggt catcgggcgc atcaaccgcg aattgagcgc
     1621 cgccatgggc gcggatgcga tgcgcgacgc gctcaccgat cagggtttcg tgccgcgccc
     1681 gctcggtccg gatgatgcgg cgcggctgct gcgcgaagag accgcacgct ggcgcaaagt
     1741 gatcgtggac gccggcatca agtccgaatg agtcgcctgc cccgggcagc gatcaggccg
     1801 cgattctgcg ccgtgcgcgg cttcggagat actccaccac acccggcatg atcgaaagga
     1861 tcacgattcc gatgatgacc aagctcaagt tgtccttcac caccggcagg ttgccgaaga
     1921 agtatccggc ataggtgagt gaaccgatcc agaagacgct ccccgcaaag ctgaagaaca
     1981 tgaaacgcgg atagggcatg cgcccgatgc ccgccacgaa cggcgcgaag gtgcgcacga
     2041 tcggtatgaa gcgcgcgatg acgatcgtct tgccgccgtg ccgctcgtag aaacgccgcg
     2101 tgcgctcgag atgcgccgga ttgagagccc acgactgctg ttgcgaaaag acccgcgggc
     2161 cgacgaagcg tccgatccag taattcgtgt tgtcgcccga gaaggatgcc agcacgagca
     2221 gcgcgaaaag accgtgtatg tacatgtcgc ccgtggccgc gagcgttccg gcgacgaaaa
     2281 gcagcgaa
//
LOCUS       DASURG010000134         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_128479, whole genome shotgun sequence.
ACCESSION   DASURG010000134 DASURG010000000
VERSION     DASURG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128479"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1179)
                     /locus_tag="VFY80_02210"
     CDS             complement(49..1179)
                     /locus_tag="VFY80_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEX6006745.1"
                     /translation="MQATRIRQKDGIFYFASYPAADILSKVRFISRYYGEGEEITPQS
                     VPHNDEIAQFIARIERTDEAFQRQLSRAKVRSLKNFYEMAVSQPPIPGTVLLFTGETL
                     RFTAGGSNGDVGSLQEPDSKYLIIDGQHRLAALRFYLREHPTEAASISVPCIIFDGRS
                     EDFAAEMFVIINSTPTRINKSHLVDLYERVSWADPDRRFAARMVEDLYSEGDSPLRYR
                     INRLGGRSQKDKWILQAELFNELHRWVSADWKKIRQSSNTYREEERYYSVIRDFLKAA
                     QKVWDDAWGHDGYMVTKPVTLKAMVRVCADLARQDAEPAEGRIKRWEERLAPWGDMVR
                     SFRVEGFYERFPAKGQIERVARIHRDLAKAAGIETRSAKNAG"
     gene            complement(1414..1671)
                     /locus_tag="VFY80_02215"
     CDS             complement(1414..1671)
                     /locus_tag="VFY80_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006746.1"
                     /translation="MAMLGLAAVVLTQPVVAAPWDSRPGLQSQDRRNPPRQHQSERRD
                     QRAAPQRDDRQRQRLSDEERRDLRRDLDRANRELYRRNRQR"
     gene            1952..2683
                     /gene="ompR"
                     /locus_tag="VFY80_02220"
     CDS             1952..2683
                     /gene="ompR"
                     /locus_tag="VFY80_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX6006747.1"
                     /translation="MPENKTKILVVDDDLRLRDLLNRYLTEQGFSVRAVADATDMNRH
                     LARERYDLMVLDLMLPGEDGLSICRRLRGSNETMPIIMLTAKGDDVDRIVGLEVGADD
                     YLAKPFNPRELVARIQAVLRRRPAPQPPGAPSPDTQVVEFGEFSFNLATRSLSRNGDD
                     VPLTTGEFALLKVLVQHPRTPLSRDKLMELARGREFGAFDRSIDVQVSRLRRLIEPDP
                     AKPAFIQTVWGFGYVFIPDGRNREQ"
     gene            2734..>3275
                     /locus_tag="VFY80_02225"
     CDS             2734..>3275
                     /locus_tag="VFY80_02225"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX6006748.1"
                     /translation="MSALARLLPRTLLTRSVLMITLILVVAHLAWLQIFRVTEREPRA
                     RQVAQQIVSVVNLTRAALVTSQPDKRFSLLAELSRSEGIQVYVSEPDEIVPPLPDRPF
                     LRIVATELREELGPDTRLAVSRSGVRGVWVSFKIDEDEYWVMLPRTRIERNEPLRYIG
                     WGLFVLLVALAGAYFMVARI"
BASE COUNT          536 a         1050 c         1061 g          628 t
ORIGIN      
        1 gacatccgcg tcgagatcat ggtgacctgc gcgcggtaga gccggagtct agcccgcgtt
       61 cttagcgctg cgggtttcga tgcccgcggc cttggccaga tcgcggtgga tgcgcgcaac
      121 ccgctcgatc tggcccttcg cgggaaagcg ctcgtagaag ccctcgacgc ggaatgagcg
      181 caccatgtcg ccccacggtg cgagccgctc ctcccagcgt ttgatgcggc cctcggccgg
      241 ttccgcgtcc tggcgcgcga ggtcggcgca tacgcgcacc attgccttca gggtgacggg
      301 cttcgtgacc atgtagccat catggcccca ggcatcgtcc cataccttct gcgcggcctt
      361 gaggaaatcg cggatcacgc tgtagtagcg ctcttcctcg cggtaggtgt tcgagctctg
      421 gcggattttt ttccagtcgg cgctcaccca gcggtgcagc tcgttgaaaa gctccgcctg
      481 gagtatccac ttgtccttct ggctgcggcc acccagccgg ttgatgcggt agcgcaacgg
      541 actgtcgcct tcgctgtaga gatcttcgac catgcgcgcg gcaaagcgcc ggtcgggatc
      601 cgcccacgac acccgctcgt agagatccac gagatggctc ttgttgatgc gggtgggcgt
      661 tgaattgatg atgacgaaca tctccgcagc gaagtcctcg ctgcggccgt cgaagatgat
      721 gcagggcacg ctgatgctcg cggcctcggt cggatgctcc ctgagataga agcgcagcgc
      781 cgccagccga tgctggccat cgatgatgag gtacttggaa tcgggctcct ggaggctgcc
      841 gacgtcgccg ttcgagccgc ccgcggtgaa gcgcagggtt tctccggtaa acaacagcac
      901 ggtgccgggg atgggcggct gcgagacggc catctcgtag aagttcttga gcgagcgcac
      961 cttggcgcgc gagagctgac gctggaaggc ttcgtcggtg cgctcgatgc gtgcgatgaa
     1021 ctgcgcgatc tcatcgttgt gcgggaccga ctgcggcgtg atctcctcac cttcgccgta
     1081 gtagcggctg atgaagcgca ccttgctcag aatgtcggcc gctggatagc tcgcgaagta
     1141 aaaaatgccg tccttctggc gtatccgggt tgcctgcatg gtttttctcc agttttctta
     1201 tgcctgcacg tcgagtccga taatcccgct gacattctag cttgactgcc ggaataacgc
     1261 gagaaaacag tcccctgcat caggttcatg gaacgcggga gaggagagcg ccatgattcc
     1321 ggcatggcgg gccgagcgct tgcgccgctg gaacgccatc ggcaagacat tgatagttaa
     1381 acgattgtcg gcgcccgtgg ggccggcgcg cgctcagcgc tgccggttgc gccggtagag
     1441 ttcccggttg gcgcggtcga ggtcgcgccg cagatcgcgg cgctcttcgt cgctcaaccg
     1501 ctggcgctgg cggtcatccc gttgcggcgc ggcgcgctgg tcgcgccgct cgctctggtg
     1561 ttgccgcggc gggttacggc ggtcctgcga ttggagcccg ggcctggaat cccacggggc
     1621 agcgacgacg ggctgcgtca gcacgacggc cgcgagtcca agcattgcca tccacttttt
     1681 catctgctga tcccctgctg ccgattgacg gcgccgtccc gggtcggcgg tccattatag
     1741 aagagccggt ctccggaact ttgacggata tgcggccatt gtccccgatg agagctgccg
     1801 tccatgtgtc gggtagacta acgcaatgac gcggaacacg tttaacaccc gggcggattt
     1861 ttgtaacaat ttgtttccct tccatgctgc gcaataaact tttacacgca cggcgcggcg
     1921 cgcaagccgg ctgccgttta ccattccggc catgccagaa aacaagacaa aaattctcgt
     1981 cgtggatgac gatctgcggt tgcgcgatct gctgaaccgt tatctgaccg agcaagggtt
     2041 cagcgtacgc gccgtcgcgg acgcaaccga tatgaaccgc catctcgcgc gcgagcgcta
     2101 cgatctcatg gtgctcgacc tcatgctccc gggcgaggat ggattgagca tctgccggcg
     2161 gctgcgcggc agcaacgaaa ccatgccgat catcatgctg accgcgaagg gggacgacgt
     2221 cgaccgcatc gtcggcctcg aagtcggcgc ggatgactac ctggccaagc ctttcaatcc
     2281 gcgcgagctg gtcgcgcgca tccaggctgt gctgcggcgc cgcccggcac cacagccacc
     2341 cggcgccccg tctccggata cccaggtggt cgagttcgga gagttcagct tcaatctggc
     2401 cactcgaagc ctcagccgca acggggacga cgtgccgctc accaccggcg aattcgcgct
     2461 gctcaaggtc ctggtgcagc acccgcgcac gccgctctcg cgcgacaagc tgatggagct
     2521 tgcgcgcggg cgcgagttcg gcgctttcga ccgttcgatc gacgtgcagg tctcgcgcct
     2581 gcgccgcctg atcgagcccg acccggccaa gccggccttc atccagacgg tatggggctt
     2641 cggctacgtt ttcatccctg acggaagaaa ccgcgagcag tgacagcggt ccgggctgat
     2701 ggcaagccga tcgccgcgaa ctcggagcgc cgggtgagcg cgctggcgag gctgctgccg
     2761 cgcacgctgc tcacgcgcag cgtgctcatg atcacgctga tcctcgtcgt cgcgcatctc
     2821 gcgtggctgc agatcttccg ggtgaccgag cgcgagccgc gggcgcgcca ggtcgcccag
     2881 cagatcgtga gcgtggtgaa tctgacccgc gccgcgctcg tcacttcgca gcccgacaag
     2941 cgcttttcac tgctcgccga gctctcgagg agcgagggca tccaggtcta tgtctcggaa
     3001 cccgatgaga tcgtgccgcc gctccccgac cggccatttt tgcggatcgt ggccacggag
     3061 ctgcgcgaag agctcgggcc cgacacccgg ctcgccgtga gccgcagcgg cgtgcgcggc
     3121 gtttgggtga gcttcaagat cgatgaagac gagtactggg tcatgctgcc gcgcacccgc
     3181 atcgagcgca acgagccgct gcgctacatc gggtgggggc ttttcgttct gctcgtggcc
     3241 ttggccggtg cctacttcat ggtggcccgc atcaa
//
LOCUS       DASURG010000135         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1457110, whole genome shotgun sequence.
ACCESSION   DASURG010000135 DASURG010000000
VERSION     DASURG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457110"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..403
                     /locus_tag="VFY80_02230"
     CDS             179..403
                     /locus_tag="VFY80_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006749.1"
                     /translation="MTTNDKRLAMLSGRMDKEAWTRLYRMQHHPKFGDVLGKLEQITG
                     TITHQHVEAAAQEFVDEPEFQQWLSGRFKP"
     gene            complement(491..1141)
                     /locus_tag="VFY80_02235"
     CDS             complement(491..1141)
                     /locus_tag="VFY80_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6006750.1"
                     /translation="MIRVVLADDHRMVRAGLKEILADTGDIEVVGEATNGLEVMARVR
                     EGSFEVLVLDMSMPGRSGIELIRQVKGEKPALRILVLSMHSEEQYAVRALKAGASGYL
                     TKEGAADELVAAIRRIAGGGAYVTPETAQRLALGLDGGREGPPHAALSDREMQVFLMI
                     TAGKTVSEVAEELILSVKTVSTHKTRIMQKLNLTNQSELIRYALRHGLIDDPAQDR"
     gene            complement(1138..>2163)
                     /locus_tag="VFY80_02240"
     CDS             complement(1138..>2163)
                     /locus_tag="VFY80_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX6006751.1"
                     /translation="AIITVDEAQNVVLFNAAAEKIFCCGSEQALERPLDRFIPARYRD
                     AHRGHVERFGRTGVTMRRMGENVVLAGLRASGEEFPIDASISQVVVGGRRFYTVILRD
                     ITERQKSAAQLEASHRELRELYGVMHEVREAERVRIARELHDELAQWLTALKMDVSWI
                     ASRLPKDLTRLSEKTAKMKEVVDTTVAAVRRIASDLRPVMIDDLGLVPAIEHLLHEFS
                     QRTGILVSLDTQTEGMEFRDPVATAVYRMVQEALTNVARHAEATRVELSVTSEDGMLV
                     VSVRDNGRGIDEERMRKAKSYGVLGIRERAQTLGGNARIYRADEGGTIVEINIPMRAQ
                     KREERAA"
BASE COUNT          393 a          719 c          614 g          437 t
ORIGIN      
        1 tgccgccctt aggagcccga gcgtgaagac cgcgggtgac tggcaggaag cgatcggatg
       61 aaggcgccgc cagtaaagaa gaagtagtca atttcccgtt ggcagtgcca tgaatagtcc
      121 tgatggcgca ctcgacaaca ctgttgtcag tcttcaccat cactctgcgg aggggaaaat
      181 gaccacgaac gacaagcgac tggccatgct gagcggcagg atggataagg aggcttggac
      241 acggctatac cggatgcagc atcaccccaa gtttggggac gttctgggca agctggagca
      301 gatcaccggc accataacgc atcagcacgt agaagccgcc gcgcaggaat tcgtagacga
      361 gccagaattc cagcagtggt tatccggtag gttcaagccg taggaaaacg acctcctcac
      421 gaagcgggtt tcttgcgtcg ggtcgtttcg taacctgcat actgtcggaa tgttccgaca
      481 atgccctttg ctagcggtcc tgggccgggt cgtctatgag accgtggcgc aacgcatagc
      541 ggataagctc gctctggttg gtgagattga gcttttgcat tatgcgagtc ttgtgcgtgc
      601 tcactgtctt gacgctcagg atcaattcct cggccacctc ggacaccgtc ttgccggccg
      661 tgatcatgag gaatacctgc atctcgcgat cggaaagcgc cgcgtgtggc ggcccttccc
      721 tgcccccgtc cagacccagc gccagacgct gggcagtctc cggagtgacg taagcacccc
      781 cacccgcgat ccggcgtatg gcggccacca gctcgtccgc tgcgccttcc ttggtgagat
      841 agcccgatgc gccggccttc agcgccctga ctgcgtactg ctcttcgctg tgcatgctga
      901 gcacgagtat ccgcagcgcc ggcttttccc cttttacctg ccggatcagt tcgatccccg
      961 agcgccccgg catcgacatg tcgaggacca gcacctcgaa cgatccctcg cgcacgcgcg
     1021 ccatcacctc cagcccgttc gtggcctcgc ccactacctc gatgtccccg gtgtcggcca
     1081 ggatctcctt gaggccggct cggaccatcc tgtggtcgtc agcgagcact acgcgaatca
     1141 agccgcacgc tcctcacgtt tttgcgcgcg catgggaatg tttatttcca ctatcgtgcc
     1201 gccttcgtct gcccggtaga tgcgggcgtt tcctcccagc gtctgcgcac gttcgcgtat
     1261 cccgagcacg ccatatgact tcgccttgcg catacgctcc tcgtctatcc cccgtccgtt
     1321 gtcacgcacg ctcactacca gcatcccgtc ctcggacgtc acgctcagtt ccacccgggt
     1381 cgcctcggcg tgacgggcga cattggtaag cgcctcctgc accatgcggt acaccgcggt
     1441 cgcgaccgga tcgcgaaact ccatgccctc tgtttgcgta tccaggctca ccaggatgcc
     1501 ggtgcgctgt gaaaactcgt gcagcaggtg ttcgatcgcg ggcaccagac cgaggtcgtc
     1561 gatcatcacg ggccgcagat cggacgcgat gcggcgcacg gcggcaaccg tggtgtccac
     1621 cacttccttc attttcgccg tcttttccga tagccgcgtc agatccttcg gcaatcgcga
     1681 cgcgatccac gacacatcca tcttgagcgc cgtaagccat tgcgccagct catcgtgcaa
     1741 ttcacgcgcg attcggacgc gctccgcctc ccgcacttca tgcatgacgc cgtaaagttc
     1801 ccgcaattcg cgatgtgatg cctcgagttg cgctgcggat ttctgccgct cggtaatgtc
     1861 tctcaggatt acggtgtaga agcggcggcc gcccaccacc acctgcgaaa tcgacgcatc
     1921 gatcggaaac tcctcgccgc tcgcacgcaa gcccgcaagt acaacgttct cccccatgcg
     1981 gcgcatggtc acgccggtcc gcccgaagcg ctcgacgtga ccgcggtgtg cgtcgcgata
     2041 gcgggccggg atgaaccgat cgagcgggcg ctcgagggcc tgttccgagc cgcagcagaa
     2101 tatcttttcc gcggccgcgt tgaaaagcac gacgttctgc gcttcatcca cggtgatgat
     2161 cgc
//
LOCUS       DASURG010000136         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_537394, whole genome shotgun sequence.
ACCESSION   DASURG010000136 DASURG010000000
VERSION     DASURG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537394"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFY80_02245"
     CDS             <1..740
                     /locus_tag="VFY80_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX6006752.1"
                     /translation="AMAVRAYRAVGKHKIGLVVNLEPKYAASQSKDDLEATRRADAYM
                     NRQYLDPVFLGRYPEELKDIFGEAWPEWPADDFRLIREPLDFLGVNYYTRNVTRHDET
                     AWPLKARPVRQKQSTYTETGWEVFPQGLRDVLLWVKGRYGNPPVYVTENGAAFYDPPT
                     ADGEELADPLRVEYLRRHLRALGEAIERGCNVRGYFAWSLLDNLEWSLGYSKRFGLVH
                     VDFASQRRTPKASARFYTSVIAAHGLH"
     gene            908..>2183
                     /locus_tag="VFY80_02250"
     CDS             908..>2183
                     /locus_tag="VFY80_02250"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX6006753.1"
                     /translation="MSARQAFPWLGLLLLLVVAAATVMQGEALMPGKPDRVPNLTHPI
                     VPAPAKTVPSVKQEATPIAVPAARPAARSLPALVNELQERTFRFFWDTADPVTGLVPD
                     RYPTPSFASIAAVGFALTAYPVGAERGYITREAARERVLRTLRFFRNAPQGAEATGTA
                     GYNGFFYRYLDMKSGHRVGDIELSTVDTALLLGGMLFCQSYFDGSESEEAEIRQLVDE
                     IYGRVNWRWVQVRGAAISHGWTPESSFLEYDWRGYNEAMLVYILALGSPTHAVAPAAW
                     NEWVREYERLWGPVYGQTHLRFESHFGHQYTHVWVDFRGIADAAMKKLGIDYFENSRR
                     ATYAQRAYAVANPMGWAGYGENIWGLTASDGSVDAELNFAGQKRRFRTYYARGIGPEP
                     IDDGTIAPTAAAASIAFAPEIVVPAIEEMHRRY"
BASE COUNT          394 a          654 c          724 g          411 t
ORIGIN      
        1 acgcaatggc cgtgcgggcg taccgggcgg tgggcaagca caagatcggg ctggtcgtga
       61 atctcgagcc caagtacgcg gcctctcagt cgaaggacga tctggaggcg acccggcgcg
      121 ccgatgcata catgaaccgc cagtatctcg acccggtgtt cttggggcgc tatcctgagg
      181 aactcaagga tatcttcggc gaagcgtggc ccgaatggcc ggcagacgat ttccggttga
      241 tccgcgagcc gctcgatttc ctgggggtca actactacac gcggaacgtg acccgtcacg
      301 atgagaccgc gtggccgctc aaggcgcggc cggtgcgtca aaaacagtcg acctacacgg
      361 aaaccggttg ggaggtattt cctcagggct tgcgggatgt gctgctctgg gtgaagggcc
      421 gctacggcaa tccgccggtg tacgtgacgg aaaacggtgc ggcgttttat gatccaccga
      481 ctgcggacgg agaagagctt gccgatccgc tgcgcgtgga gtatctgcgc cggcatctgc
      541 gtgcgctcgg cgaggcgatt gaacggggat gtaatgtgcg cggctacttt gcatggtcct
      601 tattggacaa tctcgaatgg tcgctcggtt attcgaagcg cttcgggctc gtccacgtgg
      661 attttgcgag ccagcgccgc acgccaaagg cgagtgcgcg gttttacacc tcggtgatcg
      721 ccgcgcacgg cctgcattga gggttttggg gtaaacggcg tatacagaag tcttgtccgg
      781 cgattgcggg acgggttggg tcgggtttgt cgtccagcct tgatctagat cagcgccggg
      841 ccgctgcgac agcgcataat ggaaacgcgc agtcggggcg caatacagca tggagggagg
      901 gatttcgatg tccgctcgac aagcgttccc ctggctgggg ttattgctgc tgctcgttgt
      961 ggcagcggcg acggtgatgc aaggtgaagc gctcatgccg ggcaaacccg atcgcgttcc
     1021 aaacctgacc catccgatcg tccccgcccc ggcaaaaact gtcccatcgg tgaagcagga
     1081 ggcgaccccg atcgcggtac cggccgcgcg cccggccgcg cgatcgctgc cggcgctggt
     1141 caatgagttg caggagcgca ctttccgctt tttctgggac actgcagatc ccgtgacggg
     1201 cctcgtgccc gaccgctatc ccacgccatc gtttgccagc attgcagcag tgggtttcgc
     1261 gctcaccgca tatccggtcg gcgcggaacg gggctatatc acgcgcgagg cggcgcgcga
     1321 gcgggtgctc aggacgctgc gctttttccg caacgcgccg cagggggctg aagcgaccgg
     1381 cactgccggt tacaacggct ttttctatcg ctatctcgac atgaaatccg gccatcgcgt
     1441 cggagacatc gagctttcca ctgtagatac ggcgctgctc ctgggcggga tgctgttctg
     1501 ccagtcgtac tttgacgggt cggagagcga ggaagcggag attcgacaac tcgtggacga
     1561 aatctacggg cgcgtcaact ggcgctgggt gcaggtgcgc ggcgccgcga tctcgcatgg
     1621 ctggacgccc gaatcctcgt tcctcgagta cgactggcgc ggctacaacg aggcgatgct
     1681 ggtctacatc ctcgcgctcg gctcgccgac gcacgccgtc gcacccgcgg catggaacga
     1741 gtgggtgcgc gaatacgagc ggctctgggg accggtatac ggccagacgc acctgcgctt
     1801 cgaatcgcac ttcgggcatc agtacacgca cgtatgggtc gacttccgcg gcattgcgga
     1861 cgcggcgatg aagaaactcg gcatcgatta ctttgaaaat tcgcgacggg cgacctatgc
     1921 acagcgggcg tacgccgtcg cgaatcccat gggctgggcg gggtatggcg agaacatctg
     1981 ggggctcact gcgagcgatg gttcggtcga cgccgagctt aatttcgccg gacagaaacg
     2041 ccgcttccgc acctattacg cccggggcat cggaccggaa cccatcgatg acggaaccat
     2101 cgcacctaca gccgccgccg cttccatcgc gtttgcgccc gaaatcgtcg tgcccgcgat
     2161 cgaggagatg caccgccgct acg
//
LOCUS       DASURG010000137         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1047396, whole genome shotgun sequence.
ACCESSION   DASURG010000137 DASURG010000000
VERSION     DASURG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047396"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFY80_02255"
     CDS             <1..377
                     /locus_tag="VFY80_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006754.1"
                     /translation="VLSHGVLAKIYANTLLGRIHQISDLAQLFDLDRFPGQGHLQVTP
                     EARNKCVGLVLMLKFHEYLKAHGVPGQHGVVLEEAGREAFSTMLQALHYRIIHERIFT
                     SRDHRSLIHPGIWKERVLIRES"
     gene            complement(406..>2610)
                     /locus_tag="VFY80_02260"
     CDS             complement(406..>2610)
                     /locus_tag="VFY80_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX6006755.1"
                     /translation="GRPKGVCIEHRNIVSYAAGIAARLGLRPGMGHALVSTIAADLGN
                     TAIFPALMTGGCVHIISQERAENQALLSEYFDREEIDVLKITPSHLAALQSGRNPERV
                     MPRKHLILGGEATALSRIEWLRPLSPNCEIYNHYGPTETTVGALMYRVGERVPDTQSG
                     TLPLGTPLPNTRVHVLTPDGRPAATGEQGEIYIGGAGVARGYLNRPDLTAEKFTPDPF
                     SADPDARLYRTGDLGRRLSDGSIEYCGRMDNQVKVNGYRVALEEVDQALRELGGVADA
                     VTAASDSASGSTRLAAYIIPRRAKQPLWNFPTVHLLPDGTPVAHLNRSETDYIYNEIF
                     VLQAYLRHGIAIRQGDVIVDAGANIGLFTVFANLLAPDLRTICFEPNPDAFACLQANA
                     EAWGASVICLPWGLADKEGVAELTSFEGFSLLSGFHADAAKEKSTIEAYVRNQQLTAD
                     TEEIAAQVGQLIEDRLRAHSVSARLRTLSSVIKEQGIDRIDLLKINVEKSELAVLRGI
                     DAGDWPRIRQMVVEVDLTCYLEPIVGLLERNGFDTLVEQDPLLRKTELCYVYAVRRDS
                     GIGLVREATSDNHVRAVARVRDEIMTPGSIRRLLSERLPRYMVPQSFVLMEKFPLMPN
                     GKIDRRALPSSDSTQPTVDFAQPRTDTERMLAAIWSELLHVENIGVNDDFFELGGHSL
                     LAIKMVSRVHDVFRVDVQLQDLFEYPTLSALGGIVDRVLSASGGSGALLHSH"
BASE COUNT          506 a          797 c          785 g          522 t
ORIGIN      
        1 acgtcctgag ccacggcgta ctcgcgaaaa tctatgcgaa taccttgtta gggcgaattc
       61 accagatttc cgatctggcg caacttttcg atcttgaccg cttccccggg caaggccatc
      121 tgcaagtgac gcctgaagcg cgcaacaaat gcgtcgggct cgtactgatg ctgaaatttc
      181 atgaatacct caaggcgcat ggcgtccccg gccagcacgg tgtcgtgctt gaagaggcag
      241 gtcgagaagc cttctctacc atgctgcagg ctctgcatta ccggataatc catgaaagga
      301 tattcacctc acgagatcac cggtccttga ttcaccccgg aatatggaaa gagcgcgtgc
      361 tcatacgtga atcgtgatgc tgtgggccgg ataagcgatt ccggactaat ggctgtgcag
      421 cagggcgccg gagccgcccg aggcgctcag gacacggtct actatcccgc cgagcgcact
      481 caacgtcggg tactcgaaca aatcctgcag ttgcacgtcc acccggaaca cgtcgtgcac
      541 acgcgatacc atcttgatgg ccagcagtga atgcccgccc aactcgaaga aatcgtcatt
      601 gacgccgatg ttctccacat gcagcagctc ggaccagatc gcggcgagca tgcgctcggt
      661 gtcggtgcgc ggctgagcga agtccacggt gggttgcgtg ctgtcggatg aaggaagcgc
      721 ccggcggtcg atctttccgt tcggcatgag cggaaatttt tccatgagca cgaaggactg
      781 cggaaccatg taccgcggca aacgctcgct caataacctg cgtatgcttc cgggcgtcat
      841 gatctcatcg cgcacacgag caactgcgcg cacatggttg tccgaagttg cctcgcggac
      901 aaggccaatg ccgctgtcgc ggcggaccgc gtatacatag caaagctcgg tcttcctgag
      961 caggggatcc tgttcgacca aggtgtcaaa gccgttacgc tcaagaaggc ctacgatggg
     1021 ttccaggtag caagtgaggt ccacttcgac gaccatctgc ctgatcctgg gccagtcgcc
     1081 cgcatcgatc ccgcgcagca cggcgagctc gcttttctcg acgttgattt tgagcaggtc
     1141 gatgcggtct attccctgtt ccttgatcac gctcgaaagg gtgcgcagac gggcggacac
     1201 actgtgcgcc cgcagacgat cttcgatgag ctgacccacc tgcgcggcga tctcttctgt
     1261 gtccgcagtc aactgctggt tacgcacata cgcttcgatt gtggatttct cctttgccgc
     1321 gtcggcatgg aatcccgaca acaacgaaaa tccttcgaag gacgtcagtt cggccacgcc
     1381 ttccttgtcc gcgaggcccc acggcaggca gatcacggag gcgccccagg cctcggcgtt
     1441 ggcctgcagg catgcaaatg catcggggtt gggctcgaag cagatcgtgc gcagatccgg
     1501 cgcgagcagg ttcgcgaata ccgtgaagag ccctatgttg gcgcccgcat cgacgatgac
     1561 gtcgccttgc cggattgcga tgccatgccg cagataagcc tgaaggacga aaatctcgtt
     1621 atagatgtag tccgtctcgc ttctgttgag gtgcgcgacc ggcgtgccgt cgggcaggag
     1681 gtggacggtt ggaaaattcc acaaaggttg cttcgcgcgc ctgggaatga tgtaagcggc
     1741 caatcgtgtg ctgccggagg cgctgtccga cgcagccgtg acggcgtctg cgacaccgcc
     1801 cagctcacgc agggcctggt cgacctcttc cagtgcgacg cggtagccgt ttaccttgac
     1861 ctgattgtcc atccgcccgc agtactcgat actgccatcg gacaggcgcc gtcccagatc
     1921 tcctgtccga tacagacggg catcgggatc cgcgctgaaa ggatcgggcg tgaacttttc
     1981 ggctgtcaga tccgggcggt tgagatagcc gcgcgcgacc cccgcgccgc cgatgtaaat
     2041 ttcgccctgt tcaccggttg cagcaggccg cccatccggc gtcagcacgt gaacccgcgt
     2101 gttgggaagc ggcgtcccca gcggcagcgt tccggactgc gtatcgggca cgcgctcgcc
     2161 gacccgatac atgagtgccc cgaccgtggt ttcggtgggc ccgtaatgat tgtagatctc
     2221 gcagttcggc gacagcggac gcagccattc gatgcgtgac agcgccgtcg cctcgccgcc
     2281 cagtatcagg tgctttcgcg gcatcacgcg ctctggattt ctcccgcttt gcaacgccgc
     2341 caggtgggac ggggtgattt tcagcacatc gatttcttcg cgatcgaagt attccgaaag
     2401 caacgcctga ttttccgcgc gctcctggga aatgatgtgc acgcacccgc ctgtcatcaa
     2461 agccgggaaa atcgccgtgt tgccgaggtc ggccgcgatc gtggacacga gggcatggcc
     2521 catgccgggc cgaagcccga gtcgggccgc aatgccggct gcatagctga cgatgttgcg
     2581 atgctcgatg caaacgcctt tggggcgacc
//
LOCUS       DASURG010000138         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_231217, whole genome shotgun sequence.
ACCESSION   DASURG010000138 DASURG010000000
VERSION     DASURG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231217"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /gene="fusA"
                     /locus_tag="VFY80_02265"
     CDS             complement(<1..1734)
                     /gene="fusA"
                     /locus_tag="VFY80_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6006756.1"
                     /translation="MSRKTPIERYRNIGITAHIDAGKTTTTERILFYTGVSHKLGEVH
                     DGTAIMDWMEQEQERGITITSAATTCFWKGMESNYPEHRINIIDTPGHVDFTIEVERS
                     LRVLDGACTVLCAVGGVQPQTETVWRQGNKYRVPRLFFVNKMDRSGADFMRVYDQIKT
                     RLKAMPVPIQLPIGAEEKFEGVIDLVRMKAIYWDEGTQGMKFEARDIPAAMLELCKEW
                     REKMVESAAEASEELMNKYLESGELSEAEIKQGLRLRTINNEIFPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPADIPPVKGIKENGEPDERPADDEAPFSGLAFKIMTDPFVGQLIFF
                     RVYSGVVRSGDTVYNPVKGRKERVGRILQMHANTREEVKEVCAGDIAAAVGLKEATTG
                     DTLCAPDKIITLERMEFPEPVIHVAVEPKTKADQEKMGIALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEANVGAPQVAYRETIKKVCDKVEGKFVKQTG
                     GRGQYGHVWLRVEPNPAGYEFVDAIKGGAVPREYIPAVQKGINDALPNGVLAGFPVVD
                     VKVTLFDGSYHE"
     gene            complement(1747..2217)
                     /gene="rpsG"
                     /locus_tag="VFY80_02270"
     CDS             complement(1747..2217)
                     /gene="rpsG"
                     /locus_tag="VFY80_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6006757.1"
                     /translation="MPRRREIPKREVLPDPKYTSQDVAKFINVLMTRGKKSVAERILY
                     GALDQIRKRSGKDPLEVFNQAVTNVKPMVEVKSRRVGGANYQVPVEVRPVRRVALAMR
                     WLRDAARSRNEKSMDARLASELAEAAEGRGGAMKKREEVHRMAEANKAFAHYRF"
BASE COUNT          338 a          756 c          658 g          480 t
ORIGIN      
        1 ttcatggtac gagccgtcga agagcgtcac tttcacgtcc accaccggga agccggcaag
       61 cacgccgttc ggcagcgcgt cgttgatgcc tttctgcacc gcggggatgt actcgcgcgg
      121 cacggcgccg cccttgatcg cgtccacgaa ctcgtagccg gcagggttgg gctcgaccct
      181 cagccacacg tgaccgtact ggccgcggcc accggtctgc ttgacgaact tgccctcgac
      241 cttgtcgcag accttcttga tcgtctcgcg gtaggcgacc tgcggtgcgc cgacgttcgc
      301 ttctacgccg aactcgcgct tcatgcggtc gacgatgatc tcgaggtgca gttcacccat
      361 gccggagatg atggtctgcc ccgactcttc gtcagtgcgc acgcggaacg acggatcttc
      421 ctgcgcgagc cggttgagcg cgatgcccat cttctcctgg tcggccttgg tcttgggctc
      481 taccgcgacg tggatcaccg gctcggggaa ttccatgcgt tcgagcgtga tgattttgtc
      541 cggcgcgcac aacgtgtcgc cggtcgtcgc ttccttgagg cccaccgccg cggcaatgtc
      601 gcccgcgcac acttccttca cttcctcgcg cgtattggcg tgcatctgga gaatccgccc
      661 cacccgctcc ttgcggccct tgaccgggtt gtacacggtg tcgcccgagc gcacgacgcc
      721 cgaatagacg cggaagaaaa tgagctggcc cacaaacgga tcggtcatga tcttgaatgc
      781 gagccccgag aacggcgcct cgtcgtcggc cggacgctcg tcaggctcgc cgttttcctt
      841 gatgcctttt acgggcggaa tgtccgccgg agagggcagg tagtcgatca ccgcgtcgag
      901 catcgcctgc acgcccttgt tcttgaaggc cgagccgcac agcatcggga agatctcgtt
      961 gttgatggtg cgcaaccgca ggccctgctt gatctcggcc tcgctcagtt cgccgctctc
     1021 gaggtacttg ttcatgagct cttcgctcgc ctcggcggcc gactcgacca tcttctcgcg
     1081 ccactccttg cacagttcca gcatggcggc cgggatgtcg cgggcctcga acttcatccc
     1141 ctgcgtgccc tcgtcccagt aaatcgcttt catgcgcacg aggtcgatca cgccctcgaa
     1201 cttctcttcg gcgccgatcg gaagctggat cggaaccggc atcgccttga gccgcgtctt
     1261 gatctggtcg tagacgcgca tgaaatcggc acccgagcgg tccatcttgt tgacgaaaaa
     1321 gagccgcggc acgcggtact tgttgccctg acgccacacc gtttcggtct gcggctgcac
     1381 cccgcccacc gcgcacagga ctgtgcacgc gccgtcgagc acgcgcaggc tccgctcgac
     1441 ctcgatcgtg aagtcgacgt gccccggcgt atcgatgatg ttgatgcggt gctcgggata
     1501 gttgctttcc atccccttcc agaagcacgt cgtcgccgcg gaagtgatgg tgatgccgcg
     1561 ctcctgctcc tgctccatcc agtccatgat cgcggtaccg tcgtggacct cgcccagctt
     1621 gtgcgagacg cccgtgtaga acaggatgcg ctcggtggtc gtggtcttgc cggcatcgat
     1681 gtgagccgtg atgccgatat tgcggtagcg ctcgattggg gtcttgcgtg acatcaaaaa
     1741 cctttactag aacctgtaat gggcgaacgc cttgtttgcc tccgccatgc ggtgcacttc
     1801 ctcgcgcttc ttcatcgcgc cgccgcggcc ttccgcggct tcagcgagct cgctcgcaag
     1861 ccgcgcgtcc atcgatttct cgttgcggct gcgcgccgca tcgcgcaacc agcgcatcgc
     1921 gagtgcaacc cgccgcaccg gccgcacttc gaccggcact tggtaattcg cgccacccac
     1981 gcggcggctc ttcacttcca ccatcggctt gacgttcgtc accgcctggt tgaacacctc
     2041 cagagggtcc ttgccgcttc tcttgcggat ctggtcgagc gccccgtaga ggatgcgctc
     2101 ggcgaccgac ttcttgccgc gtgtcatcag gacgttgatg aatttggcga cgtcctggct
     2161 ggtgtacttc gggtccggca acacctcacg cttggggatt tctctgcgac gtggcatgac
     2221 gactcagtcc tt
//
LOCUS       DASURG010000139         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_434958, whole genome shotgun sequence.
ACCESSION   DASURG010000139 DASURG010000000
VERSION     DASURG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434958"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFY80_02275"
     CDS             <1..466
                     /locus_tag="VFY80_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6006758.1"
                     /translation="LQLLARCAGADGMVGGQVLDLEGGARITSVVELERIHAAKTGAL
                     ISAACLFGALAAGASDALLHAFGEYGRHLGLAFQITDDVLDETASTAELGKTAGKDRD
                     GAKATYPALLGLETARQRARGEAELAARALRTAGVEDAFLQQLAAFAIERRR"
     gene            471..908
                     /locus_tag="VFY80_02280"
     CDS             471..908
                     /locus_tag="VFY80_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEX6006759.1"
                     /translation="MNFLQEAPALTIALVTLFSAQLLKFLGTWLVARRVDFTRLTGMG
                     GMPSAHAASVSALSTCVGLEMGWGSTLFGAVAFFSVLIIYDAAGIRQAASRQAQLLNQ
                     MLEDLKTHHTIRGERLVERLGHTYAEVFVGATYGVLLALLLHP"
     gene            946..>2461
                     /gene="dxs"
                     /locus_tag="VFY80_02285"
     CDS             946..>2461
                     /gene="dxs"
                     /locus_tag="VFY80_02285"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX6006760.1"
                     /translation="MSLLERINSPADLRKLSEDQLETVAAEVRERMVDVISRVGGHFA
                     PGLGVVELTIALHYVFQTPVDKIIWDVGHQGYPHKILTGRNASFDRIRQKDGPSGFLM
                     RHESEYDVFGAGHAATSISAALGMAAARDLNGDKFDVVAVIGDGALTSGIAYEAMNNA
                     GHSDRDVIVILNDNEMSISRNVGAIHKYLTRIVTNPLYNKLRDTVKDVLHKSETMEAF
                     ALKLEESAKTLLTPGTLFEELGFRYVGPLDGHDLPTLIAALRQVKNWHGPRLLHVITK
                     KGKGYPVAEQNPVVWHGATPFDKISGEMAQKKGGLPAYTNVFGKGLVELGAQYPKLAV
                     ITAAMPSGTGTGLFGDAYPDRYFDVGIAEGHAITFAAGLAVEGIRPVAAIYSTFLQRA
                     YDSIIHDVAIQELPVVFAMDRAGLVGNDGPTHMGLYDIAYMLAVPNMTITAPKDGAEM
                     LALLRLGVEHTTGPFCLRYPRDNVPAPVPATSEIPPVEYGTWEVLRTGQGVAVLA"
BASE COUNT          463 a          756 c          770 g          472 t
ORIGIN      
        1 actccagctg ctcgcgcgct gtgccggcgc cgatggtatg gtcggcggac aagtgctcga
       61 tctggaggga ggcgcgcgca tcaccagcgt tgtcgagctg gagcggattc atgcagccaa
      121 gacaggcgcg ctcatcagcg cggcctgtct gttcggcgca ctggctgcgg gtgcttcgga
      181 cgccctgctg cacgccttcg gagagtacgg ccggcacctc ggcctggcgt ttcagatcac
      241 agacgatgtg ctcgatgaaa cggcgagcac cgccgaattg ggaaaaacgg ccggcaagga
      301 ccgcgatggc gccaaggcaa cctatcccgc gctgctcggc ctcgaaacgg cccgtcaacg
      361 ggcacgcggg gaggccgagc tggccgcgcg tgcactgagg actgccgggg tggaagacgc
      421 ctttttgcag cagctggccg cgttcgccat cgagcgcagg cggtagtaat ttgaacttct
      481 tgcaggaggc acccgccctc accattgcgc tcgtgacgct gttctcggcg cagctgttga
      541 aattcctggg cacctggctg gtggcgcgtc gcgtcgattt cacccgcctg accggaatgg
      601 gtggcatgcc ttccgcacat gcagcatcag tgtcggcact ctcgacctgt gtggggttgg
      661 aaatggggtg gggctccacg ctgttcggag cagtcgcctt tttttcggtg ctgatcattt
      721 acgacgcggc cggcattcgg caggcagcca gccgtcaggc acagctgctc aaccaaatgt
      781 tggaagactt gaaaacacac cacacgattc gtggcgaacg attggtagaa cgtctgggac
      841 acacgtacgc ggaagtgttc gtcggggcga cctatggcgt gctcctcgcc ttgttgctcc
      901 atccgtagca cgtgcacgga tgctcaggtg attaggattt gattgatgtc attactggaa
      961 cggatcaata gcccggccga cctgcgcaag ctgtcggagg atcaactcga aaccgtggcc
     1021 gcggaagtac gcgagcgcat ggtcgatgtc atttcacgcg tgggcggcca tttcgccccc
     1081 ggactcggtg tagtcgagct gaccatcgcc ctgcattacg ttttccagac gccggtcgac
     1141 aaaatcatct gggatgttgg ccatcagggc tacccgcaca aaattctgac cgggcgtaac
     1201 gccagcttcg atcgcattcg ccagaaggat gggccgtcgg gcttcctgat gcggcacgaa
     1261 tcggaatacg acgtcttcgg cgccggacac gccgcgactt ccatctccgc tgcgctgggc
     1321 atggccgcag cgcgcgatct gaatggtgac aagtttgatg tcgtggccgt catcggcgat
     1381 ggcgccctca cctccggcat cgcttacgag gcgatgaaca acgccgggca cagcgatcgc
     1441 gacgtcatcg tcatcctgaa cgacaacgag atgtcgatct cgcgcaatgt cggcgcgatc
     1501 cacaagtatc tcacgcgcat cgtcaccaat ccgctctaca acaagctgcg cgataccgtc
     1561 aaggatgtgc tgcataagtc cgagaccatg gaagccttcg cgctcaagct ggaagagagc
     1621 gccaagacgc tgcttacacc cggcacgctg ttcgaagagc tgggattccg ttatgtcggc
     1681 ccgctcgacg gtcacgatct gcccactttg atagccgcat tgcgccaggt gaagaactgg
     1741 catgggccgc gcctcttgca cgtgatcacc aagaaaggaa agggctaccc cgtcgccgag
     1801 cagaatccgg tcgtatggca tggtgctacg cctttcgaca agatcagcgg cgagatggcg
     1861 cagaagaaag gcggtctgcc cgcatacacc aatgtgttcg gcaagggact ggtcgagctg
     1921 ggtgcgcagt atccgaaact agccgtaatt actgcggcca tgccgagtgg taccggcacc
     1981 ggtctgttcg gtgatgcgta tcccgatcgc tatttcgatg tcggcatcgc cgaaggccat
     2041 gccatcacct ttgccgccgg cctcgccgtc gaaggcattc gcccggtcgc cgctatctac
     2101 tcgacgttcc tgcagcgcgc atacgattcc atcattcacg atgtggcgat ccaggagctg
     2161 cccgttgttt ttgccatgga tcgcgccggg ctggtgggga atgacggtcc cacgcacatg
     2221 ggactgtacg acattgccta catgcttgcg gtccccaaca tgacgatcac cgcacccaag
     2281 gatggcgccg agatgctggc gctgctgcgc ctgggcgtgg agcacaccac cgggccgttc
     2341 tgcctgcgct atccgcgcga caatgtgccc gccccggtgc cggccaccag cgagatcccg
     2401 cccgtggaat acggcacgtg ggaagtgctg cgcaccggac aaggcgtggc cgtgctggcc
     2461 g
//
LOCUS       DASURG010000140         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_129237, whole genome shotgun sequence.
ACCESSION   DASURG010000140 DASURG010000000
VERSION     DASURG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129237"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFY80_02290"
     CDS             complement(<1..715)
                     /locus_tag="VFY80_02290"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX6006761.1"
                     /translation="MAKNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGRKTVLHLIPSGILRDRVACYIGNGVVLSPLALIEEISMLENAGIDVTARLKISE
                     ACALILPNHAAVDQAREAAKGNMAIGTTGRGIGPAYEDKIARRAIRVQDLFHRKRFAA
                     KLGELLDYHNFLLKHYYKAKTVDFGQVLDETLALADRIAPLVGDVPRLLYEAQQAGES
                     LLFEGAQGTLLDVDHGTYPY"
     gene            complement(734..1891)
                     /locus_tag="VFY80_02295"
     CDS             complement(734..1891)
                     /locus_tag="VFY80_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX6006762.1"
                     /translation="MRRWLLPEYMEDILPGEAHQIERLRRRILDLFRAHGYELVMPPL
                     LEYLDSLLTGTGHDLDLQTFKLVDQLSGRMLGVRADITPQVARIDAHLLNRRGVTRLC
                     YCGSVLHTLPNAMNRTREPLQIGAEIYGHRAVESDIEIQRLMMKALAASGVNAAYLDV
                     GHVTVFRSLIRRAEAGRELETELFRAMQLKDVPEIRRLCRAFDGRTRDALVLLPELYG
                     GPEVLRLAHRKLPAYPEIRAALRTLAALSQRLRDIAHIRCFDLAELRGYYYHSGVVFA
                     AYAEGSSNALALGGRYDEVGKAFGRARPATGFSMDLRELASAAPGGAPHSGILAPYQP
                     GNAALQRRIDALRGRGEIVIEDLPGHSSSRSELNCGRRLVRRAGRWLVEKV"
     gene            complement(1899..2087)
                     /locus_tag="VFY80_02300"
     CDS             complement(1899..2087)
                     /locus_tag="VFY80_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX6006763.1"
                     /translation="MTTILLTALALMLVIEGVLPFLLPALWRQTFRKLTEMSDGQIRF
                     IGLTSMLAGLLLLYLVRH"
     gene            complement(2136..3020)
                     /gene="hflC"
                     /locus_tag="VFY80_02305"
     CDS             complement(2136..3020)
                     /gene="hflC"
                     /locus_tag="VFY80_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX6006764.1"
                     /translation="MKASLGTVLIAIIGALVIAALSMFVVDQRQHALVFRLGEVIDVK
                     RTPGLYFKVPLLDNVRYFDVRILTIDTPEAERFLTSEKKNVLVDLFVKWRIIDVRQYY
                     VSVRGEESAAQTRLQQTINDGLRAEFGNRTVHEVVSGERDQIMELMRKKANEDAKTIG
                     VEVIDVRLKRVDLPQEVSEAVYRRMEAERKRVANELRSTGGAESEKIRADADRQREVI
                     LAEAYRDAQRIRGEGDARATAIYAKAYEPNAEFYSFYRSLEAYKQSFKNRSDVLVLEP
                     NSEFFRYLRSSGRSGAAK"
BASE COUNT          546 a         1042 c          985 g          566 t
ORIGIN      
        1 cataaggata ggtgccgtga tccacgtcga gcagcgtacc ctgcgcgccc tcgaagagca
       61 ggctctcgcc cgcctgctgg gcctcgtaga gaaggcgcgg gacgtcgccc accagcggcg
      121 cgatgcggtc ggcaagggcg agtgtttcat cgagcacctg gccgaagtca acggtcttcg
      181 ccttgtaata gtgcttcaac aggaagttgt gatagtcgag cagttctccc agctttgccg
      241 caaaacgctt gcgatgaaaa agatcctgca cgcgtatcgc gcgacgcgcg atcttgtcct
      301 cgtaggcggg gccgatgccg cggccggtcg tcccgatcgc catgttgccc ttggccgctt
      361 cgcgtgcctg gtccacggca gcatggttcg gcaggatcag cgcgcaggcc tcgctgatct
      421 tgagccgcgc cgtcacatcg atgcccgcgt tttccagcat ggagatttct tcgatgagcg
      481 caagcggcga gagcacgacc ccattgccga tgtaacatgc cacccgatcg cgcaggatgc
      541 cggacggaat gagatgcagc acggtcttcc ggccgccgat gacaagcgta tgcccggcgt
      601 tgtggccgcc ctggaagcgc accacgccct gggcatgatc cgtcagccag tcgacgatct
      661 tgcctttgcc ttcgtcaccc cactgggtgc cgatcacgac cacgttcttt gccatacccg
      721 agattgtcct tgactagacc ttttcaacca accagcggcc cgcgcggcgc acgagacggc
      781 gcccgcagtt caactccgat cgcgagctgg aatgaccggg gaggtcctcg atgacgattt
      841 ccccgcgacc gcgcaacgcg tcgatgcgcc gctgcaaggc ggcgttaccc ggctgatacg
      901 gcgcaaggat accggaatgc ggcgcgcctc cgggcgcggc ggaagcaagt tcgcgcaggt
      961 ccatgctgaa accggtcgcc ggtcgggcgc ggccgaacgc cttgccgacc tcgtcgtagc
     1021 gtccgcccag cgcgagcgca ttcgatgagc cttcggcata cgccgcgaac acgaccccgc
     1081 tgtgataata gtagccgcgc agttcggcca gatcgaagca gcggatatgc gcgatgtcgc
     1141 gcagccgctg cgatagagca gccagcgttc tcagcgccgc acggatctcg ggataggcgg
     1201 ggagcttgcg gtgggcgagc cgcagcacct cgggaccgcc gtagagctcg ggaagcagca
     1261 cgagagcgtc ccgtgtccgg ccatcgaacg cacggcacaa gcgccggatc tccggcacat
     1321 ccttcaactg catcgcacga aaaagctcgg tttcgagctc ccgccccgct tccgcgcgcc
     1381 ggatcaagct gcggaaaacg gtgacgtgac cgacgtcgag gtaggctgca ttcacgccgc
     1441 tcgccgccag cgccttcatc atcagacgct ggatctcgat atcgctctcg accgcgcgat
     1501 gaccgtagat ctccgcgccg atctgcagcg gctcgcgcgt gcgattcatt gcgttcggca
     1561 gcgtgtgcag cacgcttccg cagtagcaga gccgcgtgac gccacgccga ttcaaaaggt
     1621 gcgcatcgat gcgcgcgacc tgcggtgtga tatccgcgcg cacaccgagc atgcggccgg
     1681 agagctggtc caccagcttg aacgtctgca gatcgagatc gtggcccgtt ccggtgagaa
     1741 gcgagtccag gtattccaag agcggcggca tgacgagttc atagccgtgc gcccgaaaca
     1801 ggtcgaggat acgccggcgc aggcgctcga tctggtgcgc ttcgcccggc aggatgtcct
     1861 ccatgtattc cggcagcagc caccgtctca tggctcgcct aatgccgcac gaggtagaga
     1921 agaagcagcc cggcaagcat ggacgtgagg ccgatgaagc ggatctggcc gtcggacatc
     1981 tcggtgagct tgcgaaacgt ctgtcgccac aacgccggca ggaggaaagg cagaacgccc
     2041 tcgatcacga gcatgagcgc aagtgcggtg agcaggatcg tcgtcatgcg tggctcgcgt
     2101 tcgttcccgc ggatccttcc cccgcgtccg gcccgtcact tcgcggcgcc ggacctgccg
     2161 ctcgatctca gatacctgaa aaattcggag tttggctcga gcacgagcac gtcgctccgg
     2221 ttcttgaagc tttgcttgta cgcttcgagg ctgcggtaga acgaatagaa ttcggcgttc
     2281 ggctcgtagg ccttcgcata gatcgcggtc gccctggcgt cgccctcgcc ccggatgcgc
     2341 tgcgcgtcgc gatacgcctc ggcgaggatg acctcgcgct ggcggtcggc atctgcgcga
     2401 atcttctccg actccgcccc gccggtcgat cgcagctcgt tcgccacgcg tttgcgctcc
     2461 gcctccatgc ggcggtagac ggcttcgctc acttcctggg gcaggtcgac gcgcttcagg
     2521 cgcacgtcga tcacctcgac gccgatcgtt ttcgcatcct cgttggcctt cttgcgcatc
     2581 aactccatga tctggtcgcg ctcgcccgac acgacttcat gcaccgtgcg attgccgaac
     2641 tcggcgcgca gcccgtcgtt gatcgtctgc tgcagacggg tctgtgcggc ggattcttcg
     2701 ccccgcacgc tcacgtagta ctgccgcacg tcgataatgc gccacttcac gaagagatcg
     2761 acgagcacgt ttttcttttc cgaggtgagg aagcgctcgg cttccggcgt gtcgatcgtg
     2821 agaatccgca cgtcgaaata acggacgttg tcgaggagcg gcaccttgaa gtagagcccg
     2881 ggcgtccgtt tgacatcgat cacttcgccc agccggaaga cgagcgcgtg ctggcgctga
     2941 tcgacgacga acatgctgag cgccgcgatc acgagcgccc cgatgatggc gatcaatacg
     3001 gtacccaagc tcgccttcat cagcgcgcct cccgctcgcg gctgcggaac gcctcgcggc
     3061 tgcgcgccgt cgaaggctcg ggcgcagcct cgggcggctt ctgtgccgcg tctttcccgg
     3121 gcagcgcgcc gaccatctg
//
LOCUS       DASURG010000141         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_334345, whole genome shotgun sequence.
ACCESSION   DASURG010000141 DASURG010000000
VERSION     DASURG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334345"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFY80_02310"
     CDS             complement(<1..706)
                     /locus_tag="VFY80_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6006765.1"
                     /translation="MDSILQRDQVGAFCRHTHVALKGSVSGPLAGLTFGVKDIYDIAG
                     HRTGFGSPDWFATHAAAARTAPTVQRILNAGADMAGKTHTDELTYSLNGENPHYGTPV
                     NVNAPGRIPGGSSSGSAAAVAAGLCDFALGSDTGGSVRAPASYCGIYGIRPTHGRVSL
                     EGACPLAPSFDTAGWFAREATLLERVGQVLLGEARAQAPGRLLLARDAFELAGDAITQ
                     ALQPAVRTLAKLLGEPE"
     gene            complement(745..>2117)
                     /gene="mpl"
                     /locus_tag="VFY80_02315"
     CDS             complement(745..>2117)
                     /gene="mpl"
                     /locus_tag="VFY80_02315"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX6006766.1"
                     /translation="ASMHIHILGICGTFMGGIAALARATGHRVTGCDANVYPPMSTQL
                     AALGIEILEGYTADQARLKPDVFVIGNVVTRGNPLMEEVLDLGLPYISGPQWLAEHVL
                     HGRWVLAVAGTHGKTTTSSMLAWILESAGLAPGFLIGGVPENFGVSARLGGPDGEGGT
                     FFVIEADEYDTAFFDKRSKFVHYHARTMVLNNLEFDHADIFPDLAAIETQFHHFVRTI
                     PSNGLIVANGADANLERVLRRGCWTPIERFGGESEWSIGEPDANGDFAVSYRGMPQGT
                     VRWTLLGLHNRLNAVAAVAAARHAGVPPALAVEALGSFRNVKRRMEVRGQVNGITVYD
                     DFAHHPTAIELTLAGLRAKVNGARIIAVLEPRSNTMKLGVMKDELAGSLATADRVYCY
                     GAGLGWDARAVLAPLGAKARVCDEFERLLHEVAEDARPGDHVVIMSNGGFGGIHEKLL
                     AALGRR"
BASE COUNT          341 a          720 c          697 g          359 t
ORIGIN      
        1 cctccggctc gcccaggagc ttggcgagcg tgcggacggc aggctgcagc gcctgggtga
       61 ttgcgtcgcc ggccaactcg aatgcgtcgc gcgcgagcag aagccgcccg ggtgcctgcg
      121 cgcgtgcctc gcccagcagg acctgtccca cgcgctcgag cagcgtcgcc tcgcgcgcaa
      181 accagcccgc cgtgtcgaaa ctcggcgcca aggggcaggc gccctcaagg gacacgcggc
      241 cgtgggtggg gcgaatgccg tatataccgc agtagctcgc gggcgcgcgg accgagccgc
      301 cggtatcgct gcccagcgcg aaatcgcaaa gccccgcagc caccgctgcc gcggagccgc
      361 tcgacgagcc gccgggaatg cgcccgggag cgttgacgtt gaccggcgtg ccgtagtgcg
      421 gattttcacc gttgaggctg taggtgagct cgtcggtgtg cgtctttcca gccatgtcgg
      481 cgcccgcatt gagaatgcgc tgcaccgtgg gcgcggtgcg cgcggccgcg gcgtgcgtcg
      541 cgaaccagtc ggggctgccg aatcccgtgc ggtgcccggc gatgtcgtag atgtccttca
      601 cgccgaaggt gaggcccgct agcgggcccg agaccgagcc tttcagcgcc acatgggtat
      661 gccggcaaaa cgcacctacc tggtcgcgct gcaagattga atccatttgt ctcccctgtt
      721 ccagtgcccg tgtcccggct ctgttcagcg ccggcccagc gccgcgagca gcttttcgtg
      781 tatgccgccg aatccgccgt tgctcatgat gacgacatgg tcgccgggtc gcgcatcctc
      841 cgcgacttcg tggagcagcc gctcgaattc gtcgcacact cgtgctttcg ccccgagggg
      901 cgcgagtacc gcgcgcgcgt cccagccgag ccctgcgcca taacagtaca cccggtccgc
      961 ggtcgccaga cttcccgcga gctcatcctt cattacaccg agcttcatgg tgttggaacg
     1021 cggttccagc accgcgataa tgcgcgcccc gttcactttc gcgcgcaggc cggcgagcgt
     1081 gagctcgatc gcggtgggat ggtgtgcgaa atcgtcgtag accgtgatac cgttcacctg
     1141 cccgcgcact tccatgcggc gctttacgtt ccggaacgac ccgagcgctt ccaccgccag
     1201 cgccggcggc acgcccgcat gacgcgcggc ggccactgcc gcgaccgcgt tcaggcgatt
     1261 gtgcagcccg agcagagtcc agcgcaccgt gccctgcggc atgccgcgat aggacacggc
     1321 gaaatcgccg ttcgcatccg gttcgccgat gctccattcc gattcgccgc cgaaacgctc
     1381 gatcggcgtc cagcagcccc ttcgcaacac gcgctcgaga ttcgcatcgg ccccgttcgc
     1441 cacgatgaga ccgttcgagg gaatcgtgcg cacgaaatgg tggaactgcg tttcgatcgc
     1501 cgcgaggtcc ggaaagatgt cggcgtgatc gaattcgagg ttgttgagga ccatcgtgcg
     1561 cgcgtggtag tgcacgaact tggagcgctt gtcgaagaaa gcggtgtcgt actcgtcggc
     1621 ttcgatgacg aagaacgtcc cgccttcgcc gtccggccct ccgagccggg ccgatacgcc
     1681 aaagttttcc ggcacgccgc cgatcaggaa gcccggagcg agcccagcgg attcgagtat
     1741 ccaggcgagc atcgacgaag tggtggtctt gccgtgcgtg cctgcgaccg cgagcaccca
     1801 tcgcccgtgg agcacgtgct ccgcgagcca ctgtggtccc gaaatgtagg gcagccccag
     1861 atcgagcact tcctccatta acggattgcc ccgtgtcacg acgttgccga tcacgaagac
     1921 gtccggcttg agccgggcct gatccgccgt gtagccctcg aggatttcaa tgcccagagc
     1981 ggcgagctgt gtgctcatgg ggggatagac gttcgcgtcg cacccggtga cacggtggcc
     2041 agtggcgcgc gcgagcgccg cgataccgcc catgaacgtg ccgcagatcc cgaggatatg
     2101 gatgtgcatg gaggctg
//
LOCUS       DASURG010000142         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1458109, whole genome shotgun sequence.
ACCESSION   DASURG010000142 DASURG010000000
VERSION     DASURG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458109"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFY80_02320"
     CDS             complement(<1..866)
                     /locus_tag="VFY80_02320"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX6006767.1"
                     /translation="MQKKGLQTFLYSTAGVIALAIILIAGNYIFSPVRARVDLTEGKV
                     FTLSEGTRAILTKLESPVKIRYYYTQGEATPVGLRTYAQRVEDLLNEFAAVSDGKVVI
                     EKYNPLPDSDAEESAMLDGIEGQMLNTGEKFYLGLSVSQLDDKVAIPVLTPERERLLE
                     YDLTRAVARVSTPKKPVVGVMSAVPVFGRGLSPLALQEKKGLTEPWVLISELQRDFEL
                     KEVSMTAEKIDPEIRVLLVIHPRDITETTQYAIDQFLMRGGKLIAFVDPYMYFDQQRD
                     MQNPFGGTQAGQS"
     gene            complement(876..1598)
                     /locus_tag="VFY80_02325"
     CDS             complement(876..1598)
                     /locus_tag="VFY80_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6006768.1"
                     /translation="MTIVKRELVGYFVSPVAYVFLVIFLLLTGFFTFTVGNFFERGEA
                     SLVSFFTWHPWLYLFLVPAVGMRLWSEERRLGTMELLLTLPIATWQAIVGKFLASWIF
                     LAIALALTFPVILTVNWLGDPDNGVIASGYVGSLLLAGSYLAVSCMTSAMTRNQVISF
                     ILSVLILLFLILCGYNPVTDLLSRWASPELVQVVASFSVMTHFDAFQRGILDLRDVVF
                     FASLIGFALFTTGVIIRGLRAG"
     gene            complement(1610..2575)
                     /locus_tag="VFY80_02330"
     CDS             complement(1610..2575)
                     /locus_tag="VFY80_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6006769.1"
                     /translation="MIKVENLVKNFGPKLAVNDVSFNVERGEVLGFLGPNGAGKSTTM
                     RMVTGFIPPTSGRITVGGYDMLADPIPAKRLIGYLPENAPSYADMTVQGFLNFTAEMR
                     GISGSARKQAVNRAVEMCMLENVLYQNIDTLSKGYRHRTCLAQSIIHDPDILIMDEPT
                     DGLDPNQKHEVRNLIKRMGANKAIIFSTHILEEVDAACSRVIIIDRGKIVANGTPDEL
                     RRRSHLAGAVSVVIAGVSAAQLSEGLSSVACVTRTETLAETPEQAIVRAYPSTPEQKP
                     ELVRAVMELVAQRHWRIDDLRTEEGHFDEVFRSITLPDTMKEARP"
BASE COUNT          511 a          876 c          919 g          496 t
ORIGIN      
        1 gactggccgg cctgcgtgcc gccgaacggg ttctgcatgt cccgctgctg atcgaagtac
       61 atgtacggat cgacgaaggc gatcagcttt ccgccgcgca tcaggaactg gtcgatcgcg
      121 tattgcgtgg tctcggtgat gtcgcgcgga tggatcacga gcagcacgcg gatctcggga
      181 tcgatctttt ccgcggtcat cgagacttcc ttcagctcga aatcgcgctg gagctcggag
      241 atgagcaccc agggctcggt caagcccttc ttctcctgca gcgcgagcgg cgaaaggccg
      301 cgcccgaata ccggcaccgc gctcatcacc ccgacgaccg gcttcttcgg cgtcgagacg
      361 cgcgcgacgg cccgggtgag gtcgtattcg agcaggcgct cgcgctcggg cgtgagcacg
      421 gggatcgcca ccttgtcgtc gagctggctg accgagaggc ccaggtagaa cttttcgccc
      481 gtgttgagca tctggccctc gatgccgtcg agcatcgcgg actcctccgc gtccgaatcg
      541 ggcagcgggt tgtacttctc gatgacgacc ttgccgtcgg atacggcggc gaactcgttc
      601 agcaggtcct cgaccctctg ggcataggtg cgcagcccca ccggcgttgc ctcgccctgg
      661 gtgtagtagt agcggatctt caccggcgac tcgagcttgg tcagtatcgc gcgcgtgccc
      721 tccgagagcg tgaacacctt gccctcggtc aggtcgacgc gcgcacgtac cggcgaaaag
      781 atgtaattgc ccgcgatcag gatgatcgcg agcgcgatca cgcccgcggt ggagtagagg
      841 aaggtctgca gtcctttctt ttgcatgtgt ccctcctagc ccgcgcgcag tccgcggatg
      901 atgacgccgg tcgtgaacag ggcgaagccg atcagcgacg cgaagaacac gacgtcacgc
      961 aaatcgagga tgccgcgctg gaatgcgtcg aagtgggtca tgaccgaaaa cgatgccacc
     1021 acctggacga gctccgggct cgcccagcgc gacagcaggt cggtcaccgg gttgtagccg
     1081 cacaatatga gaaagagcag gatcagcacc gagaggatga agctgatcac ctggttgcgc
     1141 gtcatcgccg aggtcatgca gctcacggcg aggtaggagc cggcgagcag gaggctcccc
     1201 acgtagcccg aggcgatgac gccgttgtcc ggatcgccca gccagttgac cgtgaggatc
     1261 accgggaagg tgagcgccag cgcgatcgcg aggaagatcc aggaggcgag gaacttgccg
     1321 acgatcgcct gccaggtcgc gatcggcagc gtcagcagca gctccatcgt tcccagacgc
     1381 cgctcctccg accacagccg catgccgacg gcgggcacca ggaacagata cagccaggga
     1441 tgccaggtga agaacgacac cagcgaggct tcgccccgct cgaagaagtt gcccacggtg
     1501 aaggtgaaga agcccgtgag cagcaggaag atcaccagga agacatatgc gaccggggag
     1561 acgaagtagc ccacgagctc gcgcttgacg atcgtcatga cgccaccgct catggccgcg
     1621 cctccttcat cgtgtcgggc agcgtgatgg agcggaacac ctcgtcgaag tggccttcct
     1681 cggtgcgcag gtcgtcgatg cgccaatgcc gctgggcgac gagctccatc accgcgcgca
     1741 ccagctcggg cttctgctcg ggcgtgctcg ggtaggcgcg gacgatcgcc tgctccggcg
     1801 tttccgcgag cgtttcggtg cgcgtcacgc aggcgacgct gctcaagcct tcgctgagct
     1861 gtgcggcgct gacacccgcg atcaccacgg ataccgcacc ggccaggtgc gagcggcggc
     1921 gcagctcgtc cggcgttccg ttggcgacga tcttgccgcg atcgatgatg atcacgcgcg
     1981 agcacgcggc atcgacttcc tccaggatat gggtcgagaa gatgatcgcc ttgttcgcgc
     2041 ccatgcgctt gatgaggttg cgcacctcgt gcttctggtt cggatcgagc ccgtcggtcg
     2101 gctcgtccat gatgaggatg tccgggtcgt ggatgatgga ctgtgcgagg caagtgcggt
     2161 gccggtagcc cttggagagc gtatcgatgt tctggtagag cacgttctcc agcatgcaca
     2221 tttcgaccgc gcggttcacc gcctgcttgc gtgcgctgcc ggagatgccg cgcatctcgg
     2281 cggtgaagtt gaggaagcct tgcaccgtca tgtcggcgta cgacggtgcg ttttccggca
     2341 ggtacccgat caggcgcttg gcaggaatcg gatcggcgag catgtcgtag ccgccgacgg
     2401 tgatgcgccc ggaagtcggc gggatgaagc ccgtgaccat gcgcatcgtg gtggatttgc
     2461 cggccccatt ggggcctagg aagcccagaa cttcgccccg ctcgacattg aacgaaacgt
     2521 cgttcaccgc gagcttgggg ccgaaattct tgaccagatt ttcgaccttg atcattgctt
     2581 gccctctcgt cggcgcccgg cccggcgcgc gtcccacgcg gacgtcatag cgaagtccgc
     2641 gaagccacgg catcggtatc gagcgcgctc ctcctcgtgc tcgcgcgggt tcacgtatcc
     2701 ccatgaatta ccgcgcaaac tggtaaaagc ggcgaagtct acacgaataa gctcggggag
     2761 ccaaaaattc gggacgccac gatcggattt caagtcccgg cc
//
LOCUS       DASURG010000143         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_28000, whole genome shotgun sequence.
ACCESSION   DASURG010000143 DASURG010000000
VERSION     DASURG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28000"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFY80_02335"
     CDS             complement(<1..842)
                     /locus_tag="VFY80_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6006770.1"
                     /translation="MAIVLGAQALEGVLGMKDAIDMLERMSRHEAQGRTVIAPRQTIP
                     IEGGWMRLMFAADYESGYAATKAFHMVRGVGVRYVVSLYRVNDGELLAVLDGRLITDM
                     RTGAASGVAARCLRLPSSVAVGILGSGHQARMQLEALAATLHVTKAVVYSPNAQHRHA
                     FAAEMTRRLDLPVAAVDSAEAAARGHEVVVVATNAVGAEPVLRRRWLDACRLVCAVGS
                     TRRESIEVDAATFGDAAAVIVDSLLAAEEAGDLEQALATGALKADRQVTLAQLVSQGR
                     QGLGG"
     gene            complement(845..1894)
                     /locus_tag="VFY80_02340"
     CDS             complement(845..1894)
                     /locus_tag="VFY80_02340"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6006771.1"
                     /translation="MADRNVTVALDRFTWRMLSQYVSPWEFEQLKQKIQVWDQWCEEW
                     SKAARRHADIADKAARAGNRVTAGDAYVRAALYYHWASFLFVHDQKQWLAAMKATDAC
                     WKKAAPLVSPPMELFEIPFEGVQLPGYLRKPDGVKKPPLVILVPGGDSTKEELYDFGE
                     HILKRGIAVFAFDGPGQGLVSTRMKLRPDFEVPVRAVTDFILKRDDLDASRLAIGGIS
                     YGGLFACRAAAFDERFKAVVSVSSWYTPAGRFPGMDPLSQTGLKQYMGDDPQSVQDSI
                     TMKGAAERIKVPLLQIYGGLDKASPPEHAHRVAAEVKGPKTTLVFDDGVHVCNNLHHI
                     VRPLIADWLAEQLKQ"
     gene            complement(1923..>2759)
                     /locus_tag="VFY80_02345"
     CDS             complement(1923..>2759)
                     /locus_tag="VFY80_02345"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6006772.1"
                     /translation="IFCTGTMGEFWALTKEERLRTVEIVVEEARGKCKVIAHTGHHSP
                     NETVDLTRHAQDVGADFAIVINPYYPAANEEMIYQWFAFVASKVEIGIWMFDTGYAGY
                     GFSPQLTARIADIENVCGIKCSRPLEHYAKVQKLCGAKIVMSHPAESEWLTLMRDYGA
                     RVHMSSAAPFLLQLPGWRPMHEYTELALQGRFTEAEAASKPLDPLRHVHERWMRDPWT
                     QHKIIPIAYLKAWTELLGLAAGPVRPPLLPITSAERKALQADLTEAGLLARLPAAQVR
                     AA"
BASE COUNT          446 a          951 c          885 g          477 t
ORIGIN      
        1 ccgcccagcc cctgtcgccc ctgcgacacc agttgcgcaa gggtcacctg gcggtcggcc
       61 ttcagggccc cggtcgcgag cgcctgctcc agatctccgg cttcctcggc cgcgagcagc
      121 gaatccacga tcaccgcggc agcatcgccg aaggtcgcgg catcgacctc gatcgactcg
      181 cgccgcgtgc tgcccaccgc gcataccagc cggcacgcgt cgagccagcg gcgccgcagc
      241 accggttccg ccccgaccgc gttcgtggcg accacgacca cctcgtggcc gcgtgctgcc
      301 gcttccgccg agtcgacggc cgcgaccggc aggtcgaggc ggcgcgtcat ctcggcggcg
      361 aacgcatgac gatgctgcgc gttggggctg tagacgaccg ctttcgtcac gtgcagcgtc
      421 gccgcgagcg cttcgagctg catgcgggcc tggtggcccg agcccagtat gccgacggca
      481 acggacgacg gcagcctgag gcagcgtgcc gcaactccgc tcgcggcgcc ggttcgcatg
      541 tcggtgatca agcgcccgtc gagcaccgca aggagctcgc cgtcgttcac gcggtagagc
      601 gagacgacat agcggacgcc gaccccgcgc accatgtgga acgccttggt cgccgcgtac
      661 ccgctttcgt agtcggcggc gaacatgagg cgcatccagc cgccttcgat cgggatggtc
      721 tggcgcggcg cgatgacggt gcgaccttgc gcctcgtgcc gcgacatcct ctcgagcatg
      781 tcgatggcat ctttcatccc caggacgcct tcaagcgcct gcgcacccaa tacgatcgcc
      841 atgcttattg cttcagctgt tcggcgagcc agtccgcgat cagcgggcgc acgatgtgat
      901 gcaggttgtt gcagacgtgc acgccgtcgt cgaacacgag cgtcgtcttc ggtcctttca
      961 cttccgccgc gacgcgatgc gcatgctcgg ggggagaggc cttgtcgagc ccgccgtaga
     1021 tctgcaggag cggcaccttg atgcgctcgg cggcgccctt catcgtgatg ctgtcctgca
     1081 ccgactgcgg atcgtcgccc atgtactgct tgagccccgt ctgcgaaagc gggtccatgc
     1141 cgggaaagcg tcccgcgggg gtataccagc tgctcaccga aaccaccgcc ttgaagcgct
     1201 cgtcgaacgc ggccgcgcga caggcgaaga ggccgccgta cgagatgccg ccgatcgcga
     1261 gccggctggc gtcgagatcg tcgcgcttca ggatgaaatc ggtgaccgcg cggaccggca
     1321 cttcgaaatc cggccgcagt ttcatgcgcg tgctgacgag cccctgcccc ggaccgtcaa
     1381 aggcgaagac ggcgatcccg cgcttcagga tgtgctcgcc gaagtcgtac agctcctctt
     1441 tggtcgagtc gccgccggga acgagaatca cgagcggcgg cttcttcacc ccgtcaggct
     1501 tgcgcaggta tccaggcagc tgcacgcctt cgaacggaat ctcgaagagc tccatcgggg
     1561 ggctgacgag cggtgcggcc ttcttccagc acgcgtccgt cgccttcatc gcggcgagcc
     1621 actgcttctg atcgtggaca aaaagaaagc tcgcccagtg gtaatagagc gctgcgcgca
     1681 cgtacgcgtc accggccgtg acgcgattgc ccgctcgagc cgccttgtcg gcaatatcgg
     1741 cgtgcctgcg cgcggctttc gaccactcct cgcaccactg gtcccagacc tggatcttct
     1801 gcttgagctg ctcgaactcc catggcgaca cgtactgcga caacatgcgc caggtgaagc
     1861 ggtcgagcgc gaccgttacg tttctgtccg ccatctctgt cctctggaaa tgtgatgcgc
     1921 gctcatgccg cgcgcacctg cgccgccggc aaacgcgcca gcagcccggc ctcggtcaaa
     1981 tcggcctgca acgccttgcg ctcggcgctc gtgatgggga gaagcggcgg gcgcaccgga
     2041 ccggcggcaa gccccagcag ctcggtccag gccttgaggt aggcgatcgg aatgatcttg
     2101 tgctgcgtcc acgggtcgcg catccagcgc tcgtgcacgt gacgcagtgg atcgagcggt
     2161 ttcgaggccg cttccgcctc cgtgaagcgc ccctgcagcg caagctcggt gtattcgtgc
     2221 attggccgcc agccgggcaa ctgaagcagg aacggcgcgg cggaggacat gtgaacccgc
     2281 gcgccgtagt cgcgcatgag ggtgagccac tcggattccg cagggtggct catcacgatc
     2341 ttcgcgccgc agagcttctg caccttcgcg taatgctcga gcgggcgcga gcacttgatg
     2401 ccgcagacgt tttcgatgtc ggcgatgcgc gcggtcagct gcggtgaaaa cccgtagccc
     2461 gcatagccgg tgtcgaacat ccagatgccg atctcgacct tcgacgcgac aaacgcgaac
     2521 cactggtaga tcatctcctc gttcgcggcc gggtaatagg gattgatgac gatggcgaag
     2581 tccgctccga cgtcctgcgc gtggcgcgtg agatccaccg tttcgttcgg cgaatggtgc
     2641 ccggtgtgcg cgatcacctt gcacttgccg cgcgcctcct ccacgacgat ttccaccgtg
     2701 cgcagccgct cttccttggt gagcgcccag aactcgccca tggttccggt gcagaaaat
//
LOCUS       DASURG010000144         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_641001, whole genome shotgun sequence.
ACCESSION   DASURG010000144 DASURG010000000
VERSION     DASURG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641001"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /gene="rplA"
                     /locus_tag="VFY80_02350"
     CDS             <1..136
                     /gene="rplA"
                     /locus_tag="VFY80_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855887.1"
                     /note="in Escherichia coli and Methanococcus, this protein
                     autoregulates expression; the binding site in the mRNA
                     mimics the binding site in the 23S rRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6006773.1"
                     /translation="ALVDAVNKARPAGAKGVYLKKLSVSSTMGVGVRVDQTSLTQQAH
                     "
     gene            499..1026
                     /gene="rplJ"
                     /locus_tag="VFY80_02355"
     CDS             499..1026
                     /gene="rplJ"
                     /locus_tag="VFY80_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX6006774.1"
                     /translation="MGLNLEQKQAVVAEISAELAKAQAVVLAEYRSLRVSEMTELRRK
                     ARSSGVYLRVLKNTLARRAVAETPFKGLLDKMVGPLAYGISADPVAAAKVLHEYAKGN
                     EKFVIRAGAMPDVVMSPKEVAALAGMPSRQELLARLMGTMQAPIAKLARTLNEVPGKF
                     VRTVAAVHDQRQKAA"
     gene            1086..1466
                     /gene="rplL"
                     /locus_tag="VFY80_02360"
     CDS             1086..1466
                     /gene="rplL"
                     /locus_tag="VFY80_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6006775.1"
                     /translation="MALARAEILDAIANMTVLELSQLIKDMEDKFGVSAAAATVAVAA
                     PATGAAAAPAEEKTEFTVMLTNAGANKVNTIKVVRAITGLGLKEAKDLVDGAPKPVKE
                     SISKADADAIAKQLTEAGATVEVK"
     gene            1615..>3205
                     /gene="rpoB"
                     /locus_tag="VFY80_02365"
     CDS             1615..>3205
                     /gene="rpoB"
                     /locus_tag="VFY80_02365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882575.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6006776.1"
                     /translation="MAYSFTEKKRIRKSFAKRDSVLPVPYLLATQLESYAAFLQEHVS
                     AENRRSEGLQAAFRSIFPIESHSKNARLEFVSFALGEPPFDVKECQQRGLTFAAPLRA
                     KVRLTIMDKEASKPTIKEVKEQEVYMGEIPLMTATGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEYDPKDYLYFRVDRRRKMPVTILLKALG
                     YTPEQILKEFFTFDTFHLSAEGIEFELVPERMRGEIARFDIVTKGGKQIVAKDKRITV
                     KHVREMEQAGLSRMSVPEDFLMGRTLGHNVVDKETGELVANANDEVTDQVLAKLRQAN
                     VERIQTIYTNDLDQGPYIAQTLRIDETADQMAAQVAIYRMMRPGEPPTEDAVKTLFNG
                     LFFSEDRYDLSAVGRMKFNRRVGRSALEGAGTLSPEDIVAVIKILVDLRNGRGEIDDI
                     DHLGNRRVRSVGELAENQFRAGLVRVERAVKERLSQAESENLMPHDLINAKPVSAAIR
                     EFFGSSQLSQFMDQTNPLSEIT"
BASE COUNT          680 a          977 c         1031 g          517 t
ORIGIN      
        1 ggcgctggtg gacgcggtta acaaggcgcg gccggccggc gcaaagggcg tgtacctcaa
       61 gaagttgagc gtctccagca cgatgggtgt gggcgtgcgg gtcgaccaga cgagcttgac
      121 gcagcaggcg cactagcgaa atcagcaatg ggtcccggat caagtccggg cttccccgga
      181 caagtccggg atgaactttg ggccggcggg catgttgccc gacgggttgt caaagaccgt
      241 aggagccgag aggtttaatt ttcgggaacg gaatgtgaag tggtgggtcg gaggctctcc
      301 ggacgccgct cccgcgattt cacagcccgc taccaaagtc ctacgcagac ggcgcacccg
      361 gaatcaggtg agtgggatgt gtactcctgg aactaaggtc gccgtatcga gaaggatgca
      421 agtgagaggc atgagggaag agatcgatgc cctcgccgcc ttggctgcat cctcgggtag
      481 ctgaatagga ggtagacctt gggtctaaat cttgagcaga aacaggcggt agtggccgaa
      541 atcagcgccg agctggcgaa ggcccaggcc gtagtgctgg ccgaataccg cagcctgcgg
      601 gtgagcgaga tgacggaatt gcgcagaaaa gcgcgcagct ccggcgttta cctgcgcgtt
      661 ctgaaaaaca ccttggcgcg ccgcgccgtc gccgaaacac cgttcaaggg gctgctcgac
      721 aagatggtcg ggccgctcgc ctacgggata tcggccgatc ccgtggcggc cgccaaggtg
      781 ctgcacgagt acgccaaggg caacgagaag ttcgtgatcc gggcgggcgc catgcccgac
      841 gtggtgatgt cgccgaaaga ggtggccgcg ctcgcgggta tgcccagccg tcaggaactg
      901 ctcgcaaggc tcatggggac catgcaggcg ccgatcgcca aactcgcgcg gacactgaac
      961 gaggtgccgg gcaagttcgt gcgtacggtg gccgccgtgc acgatcagag acagaaggct
     1021 gcgtagctcg gtgcgcgcga acgattaccg ctaacgtcaa accaacgatt taacaggagt
     1081 caggaatggc actcgccaga gcagaaattc tcgatgcaat cgccaacatg accgtgctcg
     1141 agctctcgca gctcatcaag gacatggagg acaaattcgg cgtgtctgcc gccgccgcaa
     1201 ccgtcgccgt ggctgcgccg gccaccggtg cggccgccgc gcccgccgag gaaaagaccg
     1261 agttcaccgt aatgctgacc aatgccggtg cgaacaaggt gaacacgatc aaggtcgtgc
     1321 gtgcaatcac ggggctcggc ctgaaggaag cgaaggacct cgtcgacggc gcgccgaaac
     1381 ccgtcaagga aagcatctcc aaggcggacg ccgatgcgat cgccaagcaa ttgaccgaag
     1441 ccggcgcaac ggtcgaagtc aagtaaaggc aaccaggagc acgaggccgg cggccagccg
     1501 ccggccccca tttttctttg agacggtcac gaacaagcgc acgcaacgcg gcaggtccgc
     1561 ggtgcgcacg cacgtccgtc accgccgatt cccggattat ttcgtggagt tgatatggcc
     1621 tattctttca ccgagaaaaa acgtatccgc aagagctttg ccaagcgcga cagcgtgctg
     1681 ccggtgccgt acctgctggc gacccagctc gagtcgtacg cggcgttcct gcaggaacac
     1741 gtttcggccg agaaccgcag gagcgaaggc ctgcaggcgg cattccggag catcttcccg
     1801 atcgagagtc actcgaagaa cgcgcggctc gagttcgtga gcttcgcgct cggggagccg
     1861 cccttcgacg tgaaagagtg ccagcagcgc ggtctcactt tcgccgcgcc actccgtgcc
     1921 aaggtacggc tcacgatcat ggacaaggaa gcctccaagc cgaccatcaa ggaggtgaag
     1981 gagcaggaag tgtacatggg ggagatccct ctcatgaccg cgaccggctc gttcgtgatc
     2041 aacggcaccg agcgcgtgat cgtgtcgcag ctgcaccgct ctcccggggt gttcttcgag
     2101 catgaccgcg gcaagacgca cagctcgggc aagcttctct tctccgcacg cgtcataccg
     2161 tatcgcggct catggctcga cttcgagtac gacccgaagg attacctcta tttccgcgtg
     2221 gaccggcgcc gcaagatgcc ggtcacgatc ctgctcaagg cgcttggcta cacgcccgag
     2281 cagatcctga aggaattctt caccttcgac accttccatc tgagcgccga aggaatcgag
     2341 tttgagctcg tgcccgagcg catgcgcggt gagatcgcgc gcttcgatat cgtgacgaag
     2401 ggtggcaagc agatcgtcgc caaggacaag cgcatcaccg tcaagcacgt gcgcgagatg
     2461 gaacaggcgg ggctctcccg catgagcgtg cctgaggatt tcctgatggg ccgcacgctc
     2521 gggcacaacg tcgtggacaa ggagacgggc gagctcgtcg ccaacgccaa cgacgaagtg
     2581 accgaccagg tgctcgccaa gctgcgccag gcgaacgtcg agcggatcca gaccatctac
     2641 accaacgacc tcgatcaggg cccctacatc gcccagacgc ttcggatcga cgagacggcc
     2701 gaccagatgg cggcacaggt cgcgatctac cggatgatgc gcccgggtga gcctccgacc
     2761 gaagatgcgg tgaagacgct cttcaacggt ctctttttct cggaagaccg ctacgacctc
     2821 tcggcagtcg gccggatgaa gttcaaccgc cgcgtcggcc gcagcgcact cgaaggcgcg
     2881 ggcacgcttt cgccggaaga tatcgtcgcg gtcatcaaga tcctcgtcga tcttcgaaac
     2941 ggcaggggcg agatcgacga catcgaccac ctgggcaacc ggcgtgtgcg ctcggtcggc
     3001 gagcttgcgg aaaaccagtt ccgcgcagga ctggtgcgcg tcgagcgtgc ggtgaaggag
     3061 cgcctctcgc aggccgagtc agaaaacctg atgccgcacg accttatcaa cgcgaaaccg
     3121 gtgtccgccg caatccgcga gttcttcggc tcgagccagc tctcgcagtt catggatcag
     3181 accaatccgc tctcggagat cacgc
//
LOCUS       DASURG010000145         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_844602, whole genome shotgun sequence.
ACCESSION   DASURG010000145 DASURG010000000
VERSION     DASURG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844602"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFY80_02370"
     CDS             complement(<1..299)
                     /locus_tag="VFY80_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6006777.1"
                     /translation="MDITRTLARRLVESRYEDLPQTVRHEAARALLNWLGCAIGSARH
                     ETVECALQAVRPFSGPAQAKILGRTERLDILHAALVNGLSSHVLDFDDTHARAIHP"
     gene            439..930
                     /gene="purE"
                     /locus_tag="VFY80_02375"
     CDS             439..930
                     /gene="purE"
                     /locus_tag="VFY80_02375"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX6006778.1"
                     /translation="MSAPLVGIVMGSESDWEVMKHAARQLKDFGVRFEARALSAHRTP
                     DLLIEWIERLVAGGAKCFIAGAGGAAHLAGVVASKTTLPVLAVPMPSKHLQGLDSLLS
                     MVQMPKGIPVATFAIGDAGAANAGLFAVAVLALQDRNLAAALSKFRAAQADAVKAVKL
                     PEV"
     gene            941..1831
                     /locus_tag="VFY80_02380"
     CDS             941..1831
                     /locus_tag="VFY80_02380"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX6006779.1"
                     /translation="MTTIDAPVFETDLKSLKFLHRGKVRDLYAVGEDKLLVIQTDRLS
                     AFDVVLPTPIPGKGQVLTELSLFWFKRLRDIVPNHLTGISPETVVPEGERHQVAGRSM
                     VVRKLKPLLIEAVVRGYIIGSGWSDYQKTGAVCGIRLPAGLKQAEKLPRPIFTPASKA
                     PVGEHDENITYAEAEKTIGAKYAAQVRDLSIRLYTEAAEYAATRGIIIADTKFEFGLD
                     ERGEIVLIDEALTPDSSRFWPASEYRLGVSPPSFDKQYVRDWLETQSWNKKPPAPPLP
                     ADVLAKTSQKYREALRLLTQ"
     gene            1833..2813
                     /locus_tag="VFY80_02385"
     CDS             1833..2813
                     /locus_tag="VFY80_02385"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX6006780.1"
                     /translation="MVSEADLDRAVAVLRAGGLVAFPTETVYGLGADASNDEAVRKIF
                     AAKGRPHDHPLIVHISDRDGMGRWARDIPSSARLLAERFWPGPLTLVLKHAAGVSHLL
                     TGGQDTIALRAPSHRIAQALLTRFGSAIAAPSANRYGRVSATTAEHVRQEFGSSVDFI
                     LDGGETEVGIESTIVDLSGAEPAVLRPGMIAQRALEDALGVVLGACSGSSPRAPGTLE
                     RHYAPQTPLMVMEADLMLELASSLVRQGKRVAVLARSARQPLSSAITWVAAPSDASGY
                     AHQLYANLRRLDAAGCDAILAEAPPESPEWLAIRDRLTRASAGGSPSEVT"
BASE COUNT          505 a          896 c          929 g          495 t
ORIGIN      
        1 gggtggatcg cccgggcgtg cgtgtcgtcg aagtcgagca cgtgagagct caagccgttc
       61 acgagcgcgg cgtgaaggat atcgagccgc tcggtgcgtc ccaggatctt cgcctgcgcc
      121 ggacctgaaa acgggcgcac cgcctgaagc gcgcattcga ccgtctcgtg gcgcgcgctg
      181 ccgatcgcgc acccgagcca gttcagaagc gcgcgcgctg cttcgtgccg gacagtctgc
      241 ggaagatctt cgtaacggga ttcgacgagc cgccgtgcga gggtgcgcgt gatgtccatt
      301 gcgagacggg gacagagatg gcggcgagat gcaaagcact cggcacccta tattgcgagg
      361 gcattctagc agagcgccca tgccctcaca atgcgacatt cgctacaata gcgcgctcac
      421 ttacggtgga gacttcagat gagtgcgccg ttggtcggca ttgtcatggg cagcgagagc
      481 gattgggagg tgatgaagca cgctgcccgg caattgaagg attttggtgt tcgattcgaa
      541 gcgcgcgcac tgtccgcgca ccgcaccccg gatcttctga tcgaatggat cgaaaggctc
      601 gttgcgggcg gcgcaaagtg ctttatcgcg ggcgcaggcg gcgcggcaca tctggccggg
      661 gtggtggcct ccaagacgac gctgccggtg ctcgcggtgc cgatgccttc caaacatctg
      721 cagggtctcg actcactgct ttcgatggtc cagatgccga agggcatacc ggtcgcgacg
      781 ttcgcgattg gtgacgcggg ggcggcgaac gccgggctct tcgcggtcgc cgtgcttgcg
      841 ctgcaggatc gcaacctcgc tgctgcgctt tcgaaattcc gggcggctca ggcggacgcc
      901 gtcaaagccg tcaaattgcc cgaggtatag cgagatcacc atgacgacga tcgacgcgcc
      961 cgtcttcgaa acggatctca agagcttgaa gttcctccat cgagggaagg tgcgcgacct
     1021 ctatgcggtc ggagaggaca agcttctggt gatccagacc gaccggcttt cagcctttga
     1081 cgtggtgttg cccaccccca taccgggcaa ggggcaggtg ctcacggaac tctcgctctt
     1141 ctggttcaag aggctgcgcg atatcgtccc caatcatctg acaggcattt cgcccgagac
     1201 cgtagtgccc gaaggcgagc gccatcaggt agcgggccgc tcaatggtcg tgcgaaaact
     1261 gaagccgctg ctgatcgagg cggttgtgcg cggctacatc atcggctccg gctggagcga
     1321 ctatcagaag accggggcgg tgtgcggcat caggcttcct gcgggattga agcaggctga
     1381 gaaactgccg cggcccattt tcaccccggc atcgaaagcc ccggtcggcg agcacgacga
     1441 gaacatcacg tatgccgagg ccgagaaaac gatcggcgcc aagtacgccg cgcaggtgcg
     1501 cgatctttcg attcgcctct acaccgaggc ggcggagtac gcggcaaccc gcggcatcat
     1561 catcgccgac accaagttcg agttcggctt ggacgagcgc ggcgaaatcg tgctgatcga
     1621 cgaagcgttg acgcccgatt catcgcgttt ctggccagcg tccgagtatc ggctgggcgt
     1681 gagtccaccg tccttcgaca agcagtatgt acgcgactgg cttgaaacac agtcgtggaa
     1741 caagaagccc ccggcaccgc cgcttcccgc ggacgtgctc gcgaagacgt cgcaaaaata
     1801 ccgcgaggcg ctgcgtctgc tgacgcagtg agatggtttc cgaggcggat ctcgatcgcg
     1861 ccgtcgccgt gctgcgcgca ggcggcctcg tcgctttccc caccgagacg gtatatggcc
     1921 tcggtgcgga tgcctcgaat gacgaagcgg tgagaaaaat cttcgccgcg aaaggccgcc
     1981 cgcacgacca tcctctgatt gtgcacatca gcgatcgcga tgggatggga cgctgggcgc
     2041 gcgacatccc gtcttctgct cggttgctcg cggagcgctt ctggccgggg ccgcttacgc
     2101 tcgtgctgaa gcacgctgcc ggcgtgagcc atcttctgac cggcgggcag gacacgatcg
     2161 cgctacgcgc gccctcgcac cgaatcgcgc aagcgctcct cacacgcttc ggcagcgcta
     2221 ttgccgcgcc ttcggcgaat cgctacggcc gcgtatccgc aaccacggcg gagcacgtgc
     2281 gccaggagtt tggaagctcc gtcgatttca ttctcgacgg aggcgagacg gaagtgggca
     2341 tcgagtccac gatcgtcgat ctctccggtg ccgagcctgc ggtgttgcgg cccggcatga
     2401 tcgcgcagcg cgcgctcgaa gacgcgctcg gtgtggtcct cggcgcctgc agcggctcat
     2461 cgccgcgcgc accgggaacg ctcgaacggc actacgcgcc gcaaacgccg ctcatggtga
     2521 tggaggccga cctcatgctc gaactggcgt cgagcctcgt gcgccaggga aagcgcgtcg
     2581 cggtgctcgc acgttcggcg cgacagccgc tctcgagcgc catcacctgg gtcgcggcgc
     2641 cttcggacgc ctctggatac gcccaccagc tgtacgcgaa cttgaggcgg ctcgatgcgg
     2701 cgggatgcga cgcgatactc gccgaagcgc ctccggaatc gccggaatgg ctcgccatac
     2761 gcgatcgcct cacgcgcgct tccgcaggcg gctcgccttc cgaagtgacg tagcaaacgc
     2821 tcgcc
//
LOCUS       DASURG010000146         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1048892, whole genome shotgun sequence.
ACCESSION   DASURG010000146 DASURG010000000
VERSION     DASURG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048892"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..914)
                     /locus_tag="VFY80_02390"
     CDS             complement(39..914)
                     /locus_tag="VFY80_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6006781.1"
                     /translation="MTAIKTVGVAGSGTMGAGIAIVAARAGFRTIVYDVAQAPLDRAR
                     AQTEAFFAKSVERKKLSQAQVDTILAGLTGTTDIAALGQCDIVIEAVFEDLKVKHDLL
                     RRLNEACPPSTLFASNTSTLSITEIAAGSGRDERVVGMHFCLPAQLMKLVEMSPGLRT
                     SEEAFQAAWEFCEAMGQVPVKTKDNPGFILNYFLIPFNNDVIRMVEAGLAEPADIDRA
                     IKAGLGYAMGPLELLDLVGLDTHLLVAEALYAATHEPRAAVPALVKRMIAAGHLGRKT
                     GQGFYRYGGNAMFGA"
     gene            complement(1102..2307)
                     /locus_tag="VFY80_02395"
     CDS             complement(1102..2307)
                     /locus_tag="VFY80_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6006782.1"
                     /translation="MSTAGRALHTDLPMTVFDRLDDSVVLPEAEAMVLDSVRALARDR
                     IAPRAEHYDRSGEFPWQNVEAINALGLSGMFVPEAYGGAELSYAVYLACVREISKACA
                     STGVVWATNFHAMKPLVDFGTEEQKQRLLPKVAAGALASLVITEPTAGSDATGMTTRF
                     EPRGDEIAVTGTKTFITNGAHADLMLVFGKWSEIADSRGAISALVLERGGPGLTVVRE
                     EDKMGLRASSTATLAFDGYRVPRANLLGEPGEGLKLLLASLNKSRPSVAAHALGIARA
                     AFEDSIAYINERRQSGRRIVEFQGIQFMLADMATDLAQCEAWLWHVARLVDGGARDFG
                     IEASMLKLRASDVAMRIATDAVQLHGGYGYCKDYRVERLMRDAKITQIWEGTNQVHRQ
                     LIGRSFVRR"
     gene            complement(2276..>2567)
                     /locus_tag="VFY80_02400"
     CDS             complement(2276..>2567)
                     /locus_tag="VFY80_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6006783.1"
                     /translation="CAAKAAVGAITRCLAVEWASKGISVVDVAPGYIETDLNRAALTQ
                     NPLKDLLARRIPTGGPANPDAIAKMVAAIYCENIGYLNGETIYVDGGQGIAH"
BASE COUNT          411 a          873 c          829 g          454 t
ORIGIN      
        1 tgtcgccaag cttcaggatc gaataagcaa tcgccatgtc aggctccgaa catcgcattg
       61 ccgccgtagc ggtagaagcc ctgccccgtc ttgcgcccca ggtggccggc cgcgatcatg
      121 cgcttcacca gcgcagggac ggccgcgcgc ggttcgtgcg ttgccgcgta aagcgcctcg
      181 gccaccagca ggtgcgtatc gagccccacg aggtcgagca gctcgagcgg gcccatggca
      241 tagccgaggc cggccttgat ggcgcggtcg atgtccgccg gttcggcgag tcccgcctcc
      301 accatccgga tcacgtcgtt gttgaacggg atcaggaagt agttgaggat gaacccgggg
      361 ttgtccttgg tcttgaccgg gacctggccc atcgcctcgc agaattccca cgccgcctgg
      421 aacgcctcct cgctcgtgcg caggccgggc gacatctcca ccagcttcat gagctgcgcg
      481 ggaagacaga aatgcatgcc gaccacgcgc tcgtcgcggc ccgagccggc cgcgatctcg
      541 gtgatcgaca aggtggaagt gttggatgcg aacagcgtgc tcggcggaca tgcttcgttc
      601 aggcgccgca gcagatcgtg cttcaccttc aggtcctcga acaccgcttc gatcacgatg
      661 tcgcactggc cgagcgcggc gatgtcggtc gtaccggtga gcccggcgag gatcgtgtcc
      721 acctgcgcct gcgagagctt cttgcgctcg accgacttcg caaagaacgc ttcggtctga
      781 gcgcgtgcgc ggtcgagcgg cgcttgcgcc acgtcgtaca cgatggtgcg gaagcccgcg
      841 cgcgccgcga cgatcgcgat gcccgcgccc atcgtgccgc tgcccgcgac gccgactgtc
      901 tttatggctg tcatggatgg ttaccgttca agaggtcaat cgagtttcgg ctgggttcat
      961 ttcccgtttc atttccctcc cccccccggc ccctctcccg gagggagagg ggagaatgct
     1021 attttcggaa agggaaggca cagtgtctct cgggatattt ctctcgccgc ccgtccctct
     1081 cccggaggaa gaggggagaa gctatctgcg gacgaagctg cggccgatga gctgccgatg
     1141 cacctggttc gtgccctccc agatctgcgt gatcttcgca tcgcgcatca gccgctcgac
     1201 ccggtagtcc ttgcagtagc catagccgcc gtgaagctgt acggcgtcgg tcgcgatccg
     1261 catcgcgacg tccgaggccc gcagcttcag catcgaggcc tcgatcccga aatcgcgcgc
     1321 accgccgtcg accagccgcg ccacgtgcca cagccacgct tcgcactgcg cgagatcggt
     1381 cgccatgtcg gccagcatga actggatgcc ctggaattcg acgatgcgcc ggcccgactg
     1441 gcgccgctcg ttgatgtagg caatggaatc ctcgaacgcg gcgcgcgcaa tcccgagcgc
     1501 gtgtgcggct acgctcggac gcgacttgtt gagcgacgcg agcaggagct tgagcccctc
     1561 gcccggctcg cccagcaggt ttgcgcgcgg cacgcgataa ccgtcgaaag cgagcgtcgc
     1621 agtgctcgac gcgcgcaacc ccatcttgtc ttcctcgcgc acgaccgtaa gtcccgggcc
     1681 gccgcgctcg agcacgagcg ccgagatcgc gccgcggctg tccgcgatct cgctccattt
     1741 cccgaagacc agcatgagat ccgcgtgcgc gccattggtg atgaaggtct tggtgccggt
     1801 gacggcgatc tcgtcgccgc gcggctcgaa gcgcgtcgtc atgccggtcg catccgatcc
     1861 ggcagtcggc tcggtgatca cgagcgatgc cagcgcaccg gcggccacct tgggcaggag
     1921 gcgctgcttc tgctcctcgg tgccgaagtc gacgagcggc ttcatcgcgt ggaagttcgt
     1981 cgcccagacc acgccggtgg atgcgcacgc cttgctgatc tcgcgcacgc aggccagata
     2041 gacggcgtac gacagctcgg ccccgccgta ggcttccggc acgaacatgc cgctcaggcc
     2101 gagcgcgttg atcgcctcca cgttctgcca gggaaattcg ccgcttcggt cgtagtgctc
     2161 cgcgcgcggc gcaatccggt cacgcgcgag cgcgcgcacc gagtcgagca ccatggcttc
     2221 ggcttccggg agaacgacgg agtcgtccag gcggtcgaac acggtcatcg gcaggtcagt
     2281 gtgcaatgcc ctgcccgccg tcgacataga tggtttcgcc gttgaggtag ccgatgttct
     2341 cgcagtagat cgccgccacc atcttcgcga tcgcatccgg attcgcaggc ccgccggtcg
     2401 ggatgcggcg cgcgagcagg tccttcaacg gattttgcgt gagcgccgcg cgattgagat
     2461 cggtttcgat gtagcccggc gcgacgtcca cgacgctgat ccccttggaa gcccattcca
     2521 ccgcgaggca gcgcgtgatc gcgccgacgg cggccttggc ggcacag
//
LOCUS       DASURG010000147         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_28432, whole genome shotgun sequence.
ACCESSION   DASURG010000147 DASURG010000000
VERSION     DASURG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28432"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFY80_02405"
     CDS             <1..312
                     /locus_tag="VFY80_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sphX"
                     /protein_id="HEX6006784.1"
                     /translation="PAGKAVSPSLETVVSGAYQPLSRPIFIYVNAKSLQKPEVKAFVE
                     FYNRNAERLVKEVKYVPLPAKAYSYNLETVVKARVGTKFGGENKVGLTIEELMKLEAK
                     L"
     gene            396..1298
                     /gene="pstC"
                     /locus_tag="VFY80_02410"
     CDS             396..1298
                     /gene="pstC"
                     /locus_tag="VFY80_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX6006785.1"
                     /translation="MSARLARRPLRHLRERMIEALLFCAALVSVAVTTGIVVILVKES
                     IAFFEHVSLWQFLTDTQWTPLFADAHYGILPLLSGTLVSSAVALGVAIPLGTIIAIYL
                     SEFAPHALREGAKPFLELLGGVPTIVYGYFALLFVTPLLQKIYPQLPGFNLLSAGLVM
                     GIMIIPYVSSVSEDAMRAVPMSLREGAYAMGATRFQAALGVVTPAAFSGIAAAYILGI
                     SRAVGETMIVAVAAGMQPSFTWNPLEPAATITAYIVQVALGDLPHGSVAYQTIFAAGL
                     TLALVTLFFNVMGHALRKRFRQAY"
     gene            1299..2219
                     /gene="pstA"
                     /locus_tag="VFY80_02415"
     CDS             1299..2219
                     /gene="pstA"
                     /locus_tag="VFY80_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX6006786.1"
                     /translation="MDDRLDGLRSMIAARKRWNVVFSALGVLALMVGMLTFTTLFVDM
                     LIDGLPRLSWDFFTSFPSRRPGEAGILSAWVGSTLVMLVTAAVSVPLGVAAGVYLEEY
                     APRNWVTDVIEINVANLAGVPSIVYGLLALGLFVYQFGLGQSILAAGLTLGLLVLPVV
                     IVATREAIRSIPGAIREGAYALGATPWQTTAHHILPYAMGGILTGVIIGMARAIGETA
                     PLITVGALTFIAFVPPAPLTAQAPFVSFEWLMSPFTVMPIQMFNWTSRPEAAFHHNAA
                     AAGLVLVVMTLSMNGLAIYLRYLLRRRIRW"
     gene            2213..3034
                     /gene="pstB"
                     /locus_tag="VFY80_02420"
     CDS             2213..3034
                     /gene="pstB"
                     /locus_tag="VFY80_02420"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6006787.1"
                     /translation="MVGVPRPEKRATPAAALLPEPARKASVRNLNFYYGAVQALKSIT
                     MPIYERHVTALIGPSGCGKSTLLRCFNRMHDLYPGNRYEGRIVFYPDDTNLLSPEVDA
                     IEVRMRVSMVFQKPNPFPKSIYENVAYGLRVRGVKGRSTLDEKVEEALRAAALWAEVK
                     DRLRDSAFNLSGGQQQRLCIARALATDPEMLLFDEPTSALDPIATASIEELIARLKQR
                     ITIFIVTHNMQQAARVSDYTAYMYLGELIEFGRTDDIFLNPANKQTEDYITGRFG"
     gene            3040..4152
                     /gene="phoU"
                     /locus_tag="VFY80_02425"
     CDS             3040..4152
                     /gene="phoU"
                     /locus_tag="VFY80_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX6006788.1"
                     /translation="MTMHSDREYEAELQRLHEQLLLMGARVEEMIGTSMRALTERDSE
                     LARRMIEHDHQINRLEVEIDELCLRILAKRQPVASDLRFITIALKLVTDLERIGDLGV
                     NICERVIELNLEPTLKPYVDLPKMAETVRAMVRDALDAFVAADAAHAREVIERDRTID
                     AYYAQIFRELLTYMMEDPRNIYRAIRAQSIAKYLERIGDHATNLAEMVVFMVGGKDIR
                     HLGSKEASAGHRVPRGILFLCVANSARSQMAEGWARRLSPPGVRIWSAGSQPAAGVNP
                     YAVRVMQEAGIDISMQRPKRIADVPLGDVDTVVTLCAEEVCVLPPEGLARFNWALPDP
                     ASASGSEREIEAAFRKVRDELRDRIKPLVAPAAAGA"
     gene            4190..4266
                     /locus_tag="VFY80_02430"
     tRNA            4190..4266
                     /locus_tag="VFY80_02430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4224..4226,aa:Pro,seq:tgg)
     gene            complement(4334..>4745)
                     /locus_tag="VFY80_02435"
     CDS             complement(4334..>4745)
                     /locus_tag="VFY80_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006789.1"
                     /translation="VAIAFGPDYVKREKWGHARGIIAIGNAATGWIAIGALARGFIAI
                     GGLAFGGIALGGLAVGVVGLAGVAFGALAAGGLAVGGVALGGLAIGVVAVGGVAVGTM
                     AYGGLALGESVLSGLRQDAAALQFFKQWAPWLLR"
BASE COUNT          833 a         1529 c         1509 g          874 t
ORIGIN      
        1 ccggccggca aggcggtttc accgtcactg gagacggtgg tgagcggggc gtatcagccg
       61 ctctcgcggc ccatcttcat ctacgtcaac gcaaagtcgc tccagaagcc ggaggtgaaa
      121 gcgttcgtcg agttctacaa ccgcaacgcc gagcggctgg tgaaggaagt gaagtacgtg
      181 ccgctgccgg cgaaggcata cagctacaac ctcgaaacgg tggtgaaggc gcgcgtcggc
      241 accaagttcg gcggggaaaa caaggtgggg ctcacgatcg aagagctgat gaagctcgag
      301 gcgaagcttt gaggcagcgg cggcaattga cggcgtgatt gaaaagaagc gcgttgaacg
      361 cacgaatcga aacggctgaa ttcaaatcgc acggggtgag cgctcgcctc gcgcggcggc
      421 cgctgcgtca tctgcgcgag cggatgatcg aggcgctgct cttctgcgcg gcgctcgtat
      481 cggttgcggt gacgaccggc atcgtcgtga ttctcgtgaa ggagtcgatt gcctttttcg
      541 agcacgtgtc gctgtggcag tttctcaccg atacgcagtg gacgccgctc tttgcggacg
      601 cccactacgg catcctgccg ctcctctccg gcacgctggt gagctccgct gtagcgctcg
      661 gcgtcgccat tccgctcggc acgatcatcg cgatctatct ctccgagttc gcgccgcacg
      721 cgctgcggga aggggcgaag cctttcctcg aattgctcgg aggcgtgccg accatcgtct
      781 acggatattt cgcgctgctc ttcgtgacgc cgctgctgca gaagatctat ccgcaacttc
      841 cgggattcaa tctcctctcc gcggggctcg tcatgggcat catgatcatc ccctacgtga
      901 gctcggtttc cgaggacgcc atgcgcgcgg tgccgatgag cctgcgcgaa ggtgcctatg
      961 cgatgggcgc gacgcgcttt caggccgcgc tcggcgtggt caccccggca gctttctccg
     1021 gcatcgccgc cgcttacata ctgggcattt cccgcgcggt gggggagacc atgatcgtcg
     1081 ccgtggcggc cggcatgcag cccagtttca cctggaatcc gctcgagccg gccgccacca
     1141 tcaccgccta catcgtgcag gtggcgctcg gcgatctccc gcacggcagc gtcgcctacc
     1201 agacgatctt cgcggccggg ctcacgctcg cgctcgtgac gctgttcttc aacgtgatgg
     1261 ggcacgcgct ccgcaagcgc ttcaggcagg cctactagat ggacgaccgg ctcgacggcc
     1321 tgagatcgat gattgcggcg cgcaagcgct ggaacgtcgt cttcagcgcg ctcggggtgc
     1381 ttgccttgat ggtcgggatg ctgaccttta cgacgctctt cgtcgatatg ctgatcgacg
     1441 ggcttccgcg cctttcatgg gatttcttca cgtcttttcc ctcacgccgg ccgggcgagg
     1501 cgggtattct gtccgcatgg gtcggctcga cgcttgtcat gctggtgacc gctgcagtgt
     1561 ccgtaccgct cggcgttgcg gccggcgtgt atttggagga gtacgcgccg cgcaactggg
     1621 taaccgatgt gatcgagatc aacgtggcga atcttgccgg cgtgccctcg atcgtctatg
     1681 gcttgcttgc gctgggcctt ttcgtgtatc agttcggtct gggccagagc atactcgcgg
     1741 ccggcttgac gctcggtctt ctcgtgctgc cggtcgtcat cgtcgcgacg cgggaggcga
     1801 tccgctccat tccgggggcg atccgcgaag gcgcctacgc gcttggcgcc acgccgtggc
     1861 agacgaccgc tcatcacatc ctgccgtatg ccatgggcgg catactgacg ggcgtcatca
     1921 tcggcatggc gcgcgcgatc ggcgagaccg caccgctcat caccgtcgga gcactgacgt
     1981 tcatcgcatt tgtgccgccc gcacccctga ccgcgcaagc gccgttcgtt tcattcgaat
     2041 ggctgatgtc gccgttcacc gtgatgccga tccagatgtt caactggaca tcgcgaccgg
     2101 aggccgcttt ccatcacaat gccgccgcgg cgggactcgt cctcgttgtc atgacgctct
     2161 cgatgaacgg gctggcgatt tacctccgct accttctgag gaggcggatc cgatggtagg
     2221 cgtgccgcgt ccggagaagc gggcgacacc ggccgccgcg ctcctgccgg agcccgcgcg
     2281 caaggcatcc gtgcggaacc tcaatttcta ctatggagct gtccaggcgc tgaaaagcat
     2341 cacgatgccc atctacgagc gccacgtaac tgcactcatc ggtccctccg gatgcggcaa
     2401 atcgacgctg ctccgctgtt tcaaccgcat gcatgatctc taccccggca accgctatga
     2461 gggacgcatc gtcttctatc ccgacgatac caacctgttg agcccggagg tcgatgcgat
     2521 cgaggtgcgc atgcgggtga gcatggtgtt tcagaaaccg aatcccttcc ccaaatcgat
     2581 ttacgagaat gtcgcatatg gtcttcgcgt gcgaggggtg aagggacgat ccactctgga
     2641 cgagaaggtg gaggaagcct tgcgcgctgc ggcgctgtgg gcggaagtga aggaccggct
     2701 tcgcgattcc gccttcaatc tctcgggcgg ccagcagcag cgcctctgca tcgcgcgcgc
     2761 gctcgcgacc gatccggaga tgctgctctt cgacgagccc acttcggcac tcgacccgat
     2821 cgcgaccgcg agcatcgaag agctgattgc ccggctgaag cagcgcatta ccatattcat
     2881 cgtgacgcac aacatgcagc aggcggcacg ggtctccgac tacacggcgt acatgtatct
     2941 cggcgaactg atcgagttcg ggaggaccga cgacatcttt ctcaatccgg cgaacaagca
     3001 gaccgaggat tacatcaccg gtcgtttcgg ataaccaaca tgacgatgca cagcgaccgc
     3061 gaatacgaag cggaactcca gcggctgcac gagcagctcc tcctcatggg cgcgcgggtc
     3121 gaagagatga tcggcacgag catgcgcgcg ctgaccgagc gcgacagcga gctcgcccgc
     3181 cgcatgatcg agcacgatca ccagatcaac cggctagaag tggagatcga cgagctctgc
     3241 ctgcgcatac tcgccaagcg ccagccggtc gcctccgatc tgcgcttcat caccatcgcg
     3301 ctcaagctcg tgactgatct cgagcgcatc ggtgacctcg gcgtcaacat ctgcgagcgc
     3361 gtgatcgagt tgaacctcga gcccaccctc aagccctatg tcgatctgcc gaaaatggcc
     3421 gagacggtgc gcgcgatggt gcgcgatgcg ctggatgcgt ttgtggcggc cgacgcggct
     3481 catgcccgtg aagtcatcga acgcgaccgc acgatcgacg cctactacgc gcagatcttt
     3541 cgcgagcttc tcacctacat gatggaggat ccgcgcaata tctatcgggc gatccgggcg
     3601 cagtcgatcg cgaagtatct ggagcgcatc ggggaccacg cgacgaatct cgcggagatg
     3661 gtggtgttca tggtcggggg caaggacatc cggcacctcg gcagcaagga agcgtccgcg
     3721 ggtcaccgcg tgccgcgcgg cattcttttt ctgtgcgtcg cgaattccgc gcgcagccag
     3781 atggccgaag gctgggcgcg ccggctttcc ccgccgggcg tgcgcatctg gagcgcgggg
     3841 tcgcagcccg ccgccggcgt gaatccttac gcggtgcgcg tgatgcagga ggcgggcatc
     3901 gacatctcca tgcagcgacc gaagaggatt gccgatgttc ccctgggcga tgtcgatacg
     3961 gtcgtcacgc tgtgcgccga ggaggtgtgc gtcctgccgc ccgaagggct cgcccgattc
     4021 aactgggcgc ttcccgatcc cgcgtctgcg agcggcagcg agcgggagat cgaagcggcg
     4081 tttcgcaagg tgcgggacga gctgcgcgac cggatcaagc cgctggttgc tccggccgcg
     4141 gcgggagcgt aacgcccgcg cagcttgcgg taaaatgcag gcgccggatc ggggtgtagc
     4201 tcagcctggt agagtgcttg ctttgggagc aagatgtcgg aggttcgaat cctctcaccc
     4261 cgaccagtca caccgggtcc tttctcacct tggacgcgag ccggcgcagc agtccgagag
     4321 ccattcgctt cattcagcgc aggagccacg gtgcccattg tttgaaaaac tgaagcgcgg
     4381 cggcatcctg gcgcaggccc gacagaacgc tctcgcccag agcaaggccg ccataagcca
     4441 tggtgccgac tgcgacgcca ccgaccgcga cgactccgat cgcgagtccg cccagagcga
     4501 cgccgcccac cgcaaggcca cccgccgcaa gcgcgccgaa tgctacaccg gcgagcccca
     4561 cgacgcctac cgcgaggccg cccagagcga tgccgccgaa ggcgagaccg ccgattgcga
     4621 taaatccgcg cgccagtgca ccgatggcga tccagccggt cgcagcattg ccgatcgcga
     4681 tgatgcctcg cgcatggccc cacttttcgc gcttgacgta atccggtccg aaggcgatcg
     4741 cgacg
//
LOCUS       DASURG010000148         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_641501, whole genome shotgun sequence.
ACCESSION   DASURG010000148 DASURG010000000
VERSION     DASURG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641501"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..898
                     /locus_tag="VFY80_02440"
     CDS             122..898
                     /locus_tag="VFY80_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6006790.1"
                     /translation="MNEAYTLNRASLQRKVSEEEWQVRVDLAACYRLAAQFGMTDLIY
                     THISARVPGPEHHFLINAFGFLWDEISASTLVKVTLDGEIVEDATGSGINRAGYVIHS
                     AVHSARPDVACVMHTHTQAGIAVAAQEQGLLPISQHAMRFWNGIAYHDYEGLALDMDE
                     QARLVRDLGERKAMILRNHGLLVCGASIAETFDLMYYLERACRIQVTALAGGAKLRLP
                     PEPVAEKTARQFKGLPYKPKKTEWKALLRMLDKSDPSYRE"
     gene            943..1407
                     /locus_tag="VFY80_02445"
     CDS             943..1407
                     /locus_tag="VFY80_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6006791.1"
                     /translation="MQLKSTLRVINEADYKGQRGVTEGQTYKRLIGWPEVQATDRVRI
                     GRASYLPGTYEQLHWHPIEACYYVISGHATVRDVEGREYEVSAGSIIYAPAGIAGAHE
                     WEVKEALELIDIRATNETNRKMQFTVDKKTMRSYIDLDELEKREGVNFKSHY"
     gene            1447..>2479
                     /locus_tag="VFY80_02450"
     CDS             1447..>2479
                     /locus_tag="VFY80_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6006792.1"
                     /translation="MSAQPGIPYASDLPDARLLDTRFRRCDDFNGPSAATFDAMKAEL
                     LVTSPIYPPTLAELDTQFMLHRLWQAHDRAAYIREVAPRVRGMVTTGLVGCSREWMDL
                     LPRLEIIACFGNPHGTVDLARARERGIAVTNTKDAISDTVADLAMGLVVTVMRRICEN
                     DRYVRAGKWPEGKPAMGREVGGKTCGIVGLGAIGRRIAMRAQAFRMNVAYHGPRRKAD
                     VSYPYYADPVALAEASDCLVVICPLTPETRDLVNARVLDALGPEGFLVNVARGPVVNE
                     EALISALKQKRIAGAGLDVFWDEPNVPTELLAMENVVLQPHMGSSTQEIRIERGRKLI
                     ANLRAHFSGA"
BASE COUNT          486 a          794 c          804 g          395 t
ORIGIN      
        1 gcggcgaaat tgcgcgatct ctgggcctga ttgccgcggc cgcgatgccg aatcgtccgg
       61 cgcgtgcggc gcggtatgat atgcgctccg cgcgccttcg caatcggaac gggaacaccg
      121 catgaacgaa gcctacactc tcaaccgagc gtcgctgcaa aggaaagtga gcgaagagga
      181 atggcaggtg cgcgtcgatc tcgccgcgtg ctaccggctc gccgcacagt tcggcatgac
      241 ggatctgatt tacacccaca tctcggcgcg cgttcccggg cccgagcacc actttctcat
      301 caacgcgttc ggtttcctgt gggacgagat cagcgcttcc acgctcgtca aggtgacgct
      361 cgacggcgag atcgtggagg atgcgacggg aagcggcatc aaccgcgccg ggtacgtgat
      421 tcacagcgcg gtgcatagcg cacggccgga tgtcgcgtgc gtcatgcata cccacacgca
      481 ggccggcatc gcggtggcgg cacaggagca gggcctcctg cccatttccc agcacgcgat
      541 gcggttctgg aacggcatcg cctaccacga ctacgagggc ctcgcgctcg atatggacga
      601 gcaggcgcgg ctcgtgcgcg atctgggcga gcgcaaggct atgatcctgc gcaatcacgg
      661 cttgctggtc tgcggcgcct cgatcgcaga gacgttcgac ctcatgtatt acctcgagcg
      721 cgcgtgccgc atccaggtga ccgcgcttgc gggcggcgcg aagctgcgtc ttccgccgga
      781 gccggtcgca gagaagaccg cgcgccagtt caagggcctg ccctacaagc cgaagaagac
      841 cgaatggaag gccctgctcc gcatgctcga caaaagcgat ccgtcgtacc gggagtaacg
      901 gcatcgcggc gccgacgcga catcaaccac ggaggctcaa taatgcagct caaatcgaca
      961 ctgagagtca tcaacgaggc cgactacaag ggccagcgcg gcgtcaccga gggccagacc
     1021 tacaagcggc tcatcgggtg gcccgaagta caggcgaccg accgtgtccg gatcgggcgc
     1081 gcaagctacc ttccggggac ctacgagcag ctccactggc acccgatcga agcatgctat
     1141 tacgtgattt ctggacacgc caccgtgcgc gacgtggaag gcagggagta cgaggtgagt
     1201 gcaggctcga tcatctacgc gcccgccggg atcgccggcg cgcatgaatg ggaagtgaaa
     1261 gaggcgctgg aattgatcga catccgggcc accaacgaaa ccaatcgcaa gatgcagttc
     1321 accgtggaca agaagaccat gcgctcgtac atcgacctcg atgagctgga aaaacgcgag
     1381 ggtgtgaact tcaagtccca ctattaggaa gaccgccgaa aatcgcccgg tgatccggat
     1441 ttcgtcgtgt cggcacagcc cggcatcccc tacgcaagcg atctgcctga cgcgcgttta
     1501 ctggacaccc gctttcgccg gtgtgacgat ttcaacgggc cgtcagcggc cacgttcgat
     1561 gccatgaagg cagagctgct cgtcacctcg ccgatctatc cgccgacgct ggcggagctc
     1621 gacacgcaat tcatgcttca ccggctgtgg caggcgcacg accgtgcagc gtacattcgc
     1681 gaggtcgcgc cgcgcgtgcg cgggatggtc acgaccggac tcgtcggctg cagccgggag
     1741 tggatggacc tcctgccccg gctcgaaatc atcgcgtgct tcggcaatcc gcacggcaca
     1801 gtggatctcg ctcgggcgcg cgagcgcgga attgcggtga cgaacacgaa ggatgcgatt
     1861 tccgacacgg tcgcggacct cgcgatgggc ctggtggtca ccgtgatgcg gcgcatatgc
     1921 gagaacgacc gctacgtgcg cgcggggaaa tggccggaag ggaagccggc catgggtcgg
     1981 gaagtcggcg gcaagacgtg cggcatcgta ggcctcggtg ccatcggacg acggatcgcc
     2041 atgcgcgcgc aggcgttccg catgaacgtc gcatatcacg gtccgcggcg caaggcggat
     2101 gtgagttacc cgtactacgc tgatcccgtg gcgctcgcgg aagcctccga ctgcctcgtg
     2161 gtgatctgtc cgctcacgcc ggagacccgc gacctcgtca acgcgcgggt gctcgacgcg
     2221 ctcgggcccg aaggttttct cgtaaacgtt gcgcgcggcc ccgtcgtcaa cgaggaggcg
     2281 ctcatcagcg cgctgaagca aaaacgcatt gccggggccg ggctcgacgt cttctgggac
     2341 gagccgaacg tgccgactga actcctcgcc atggagaacg tcgtgctcca gccgcacatg
     2401 ggctcgtcca cccaggaaat ccgcatcgag cgcggcagaa aactcatcgc caacctgcgc
     2461 gcgcacttct ccggagcgc
//
LOCUS       DASURG010000149         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_539123, whole genome shotgun sequence.
ACCESSION   DASURG010000149 DASURG010000000
VERSION     DASURG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539123"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFY80_02455"
     CDS             complement(<1..752)
                     /locus_tag="VFY80_02455"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831973.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX6006793.1"
                     /translation="MIEANTIAVPDYIRHKKLRGWLHEMVELCKPERVYFCDGSQAEY
                     DRLCSELVEAGTFIKLNEAKRPNSYLAWSDPSDVARVEDRTFICSRRQNDAGPTNNWV
                     APGEMKQRLQKLFDGCMRGRTLYVIPFSMGPIGSPKSHIGIEISDSAYVAVNMRIMTR
                     MGKAVYDVLGSDGEFVPCVHSVGVPLEPGQEDVPWPCNHNEKYIVHFPEERAIWSFGS
                     GYGGNALLGKKCFALRIASAMARDEGWLAEHML"
     gene            complement(749..3010)
                     /locus_tag="VFY80_02460"
     CDS             complement(749..3010)
                     /locus_tag="VFY80_02460"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX6006794.1"
                     /translation="MASRRTAALEYHEEGRPGKIAVVPTKPVGTQRDLSLAYTPGVAE
                     PCREIARDPDTVFKYTARGNLVAVVSNGTAVLGLGNLGPLGAKPVMEGKGVLFKRFAD
                     IDVFDIEVASERADEIIRFCQLLEPTVGGINLEDIAAPECFYIEEELRKTLQIPVFHD
                     DQHGTAIISGAALLNAAEVAGKKLEDLKVVFSGAGAAAIATAEHYIRLGVRREHVFMV
                     DRHGVITTDRRAELDPYKARFAQATKDRTLADVMKGADVFVGLSVAGVVTGEMVGSMA
                     TSPIIFALANPDPEITPEAIELVRKDAIIATGRTDYPNQVNNVLGFPFIFRGALDVRA
                     RSINEEMKMAATRALADLAKQDVPEAVAAAYGQTSFQFGRNYLIPKPFDPRVLLFVAP
                     AVAEAAMKTGVARMEIDQLAYRNRLEARLGRRREVMRDFILKARRSPRRIVYPEGEND
                     RIIRAAAQVVEEGIAKPILLGRAAQIKARAQALHVDLEGVQIVEHWADAERRERYAQQ
                     LHEMRQRKGVTLENAREIIRDATYFGCMMVREGDADGLIGGQEVAYPETIRPALEVVG
                     TTVNGKHVAGLYMMVLQHDVMFFADTTVNIDPDATTLAEITLLSAEFVRKLGIEPHVA
                     LLSFSNFGSSRNPQSDKVREAVRLSKLADPTLIVDGEMQADTAVVESILQKNYPFSTL
                     QEKANVLIFPTLNAANIAYKLLWRVGGAEAIGPILLGMARPVHVLQRGSEAADIVNLT
                     AIAVVDAQQRMQT"
     gene            complement(3183..>3659)
                     /locus_tag="VFY80_02465"
     CDS             complement(3183..>3659)
                     /locus_tag="VFY80_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6006795.1"
                     /translation="VNPAVANLSLGGGYSSSLNTAVTNLHNSGVFVAVAAGNSNANAC
                     NYSPASASAVYTTAASTSSDAKASYSNYGSCVDGYAPGSSIRSAWIGSTSATNTISGT
                     SMASPHVAGVAALYKGTYGNASSATIVSWINSNATTGVISGNVSGTPNRLLFKSTL"
BASE COUNT          686 a         1209 c         1058 g          706 t
ORIGIN      
        1 agcatgtgct cggccagcca gccctcatcg cgtgccatgg cggaggcaat gcgcagggca
       61 aaacacttct tgcccagcag cgcattacca ccgtagccgc tgccgaacga ccagatggcg
      121 cgctcctccg ggaaatgcac gatgtacttc tcgttgtggt tgcacggcca gggcacatcc
      181 tcctggcccg gctcgagcgg cacacccacc gaatgcacgc agggtacgaa ctcgccatcg
      241 ctgcccagca catcatacac ggctttaccc atacgcgtca tgatgcgcat gttgaccgca
      301 acgtaggccg aatccgagat ctcgatgccg atgtgtgact tgggcgagcc gatcggcccc
      361 atgctgaagg ggatgacgta cagcgtgcgt ccgcgcatgc agccgtcgaa caacttttgc
      421 aagcgctgct tcatctcgcc cggtgccacc cagttgttcg tcggtcccgc atcgttctgc
      481 cgacgcgagc aaatgaacgt gcgatcttcg acgcgtgcga catcggacgg atccgaccac
      541 gccaggtagc tgttcggacg tttggcttcg ttcagcttga taaaggtgcc ggcctcgacc
      601 agctcgctgc aaaggcgatc gtactccgcc tgcgagccat cgcagaagta cactcgctcc
      661 ggcttgcaca attccaccat ctcgtgcagc cagccacgga gcttcttgtg ccggatgtaa
      721 tcgggtacgg ctatcgtatt ggcttcaatc atgtttgcat tcgctgctgt gcgtcgacca
      781 cggcaatggc cgtcagattg acgatgtccg ccgcctcgct gccgcgttgc aggacgtgca
      841 ccggtcgcgc cattccgagc aggatcggac cgatcgcttc cgctcctccc acccgccaga
      901 gcagcttgta ggcaatgttg gccgcgttca gagtggggaa gatcagtaca tttgcctttt
      961 cctgcaatgt gctgaacggg tagttcttct gcaggatcga ctcgacgacg gccgtatccg
     1021 cctgcatttc gccgtctaca atcagggttg gatccgccag cttggacagc cggacggctt
     1081 cccgcacctt gtcactctgg gggtttcggg atgacccgaa attggagaag gatagcagcg
     1141 cgacatgcgg ctcgatgccg agcttgcgca cgaattccgc gctcagcagt gtgatctcgg
     1201 ccagcgtcgt cgcatccggg tcgatgttga cggtcgtgtc ggcgaagaac atgacatcgt
     1261 gctgcagcac catcatgtac aaacccgcga catgtttgcc attcaccgtc gtgcccacca
     1321 cttccaatgc cggacggatc gtttccggat aggccacttc ctgtccaccg atcaagccgt
     1381 ccgcatcgcc ttcccgcacc atcatgcaac cgaagtaggt tgcgtcgcgg atgatctcgc
     1441 gcgcattctc cagcgtcacg cccttgcgct gccgcatctc gtgcagttgt tgcgcgtacc
     1501 gctcacgccg ctccgcatca gcccagtgct caacgatctg cacgccttcc aggtcgacgt
     1561 gcaacgcctg cgcacgtgct ttgatttgcg cggcccgccc gagcaggatg ggtttggcaa
     1621 taccttcttc cacaacttgc gcggccgcgc gaatgatgcg gtcgttttca ccctccggat
     1681 aaacgatccg gcgcggactg cgacgcgctt tgaggatgaa gtcacgcatc acttcgcgac
     1741 gtcgtcccag ccgcgcctcc agccgattgc gatacgccag ctgatcgatc tccatgcgcg
     1801 cgacacccgt cttcatcgcg gcttccgcga ctgcaggcgc cacgaacaac agcacccgcg
     1861 gatcgaaagg cttggggatc agataattgc gtccgaattg aaagctcgtc tgcccgtaag
     1921 ccgccgctac cgcttccggc acatcctgtt tggccagatc ggccaacgca cgcgtggccg
     1981 ccatcttcat ctcttcattg atcgagcggg cccgcacatc gagcgcgccc cggaagatga
     2041 agggaaagcc aagcacgttg ttgacctgat tcggatagtc ggtgcgcccg gtcgcgatga
     2101 tcgcatcctt gcgcaccaat tcgattgctt ccggcgtgat ctccggatcg ggattcgcca
     2161 gggcaaaaat gatgggcgac gtcgccatgg agccgaccat ctcaccggtg acgacgccgg
     2221 ccaccgacaa accgacaaaa acgtcggccc ccttcatgac atcggccagc gtcctgtctt
     2281 tcgtagcttg cgcgaagcgc gccttgtaag ggtccagctc agcgcggcga tcggtggtga
     2341 tcaccccatg acgatcgacc atgaagacgt gctcgcgccg cacgcccagt cttatgtagt
     2401 gctcggccgt ggcaatcgcg gccgcgcccg cgccactgaa gacaaccttc agatcttcca
     2461 gcttcttgcc ggctacctcg gcggcattca gcaacgccgc gccgctgatg atcgcggtgc
     2521 cgtgctggtc gtcgtggaag accgggatct gcagcgtctt gcgcagctct tcttcaatgt
     2581 aaaaacactc cggcgccgca atgtcttcca gattgatgcc gcccaccgtg ggctccagta
     2641 gctggcagaa acgaatgatc tcgtccgccc gctcgctcgc gacctcgatg tcgaacacat
     2701 cgatgtccgc gaaacgcttg aagagcacac ccttcccctc catgaccggt ttggcgccga
     2761 gcggtcccag attccccagg cccagcacgg ccgtgccgtt cgacaccacc gccaccaggt
     2821 tgccgcgcgc cgtatacttg aacaccgtgt ccgggtcgcg cgcgatctcg cggcacggct
     2881 cggccacacc cggggtgtag gccaatgaca aatcccgttg cgtgccaacg ggtttagtgg
     2941 gtacgaccgc gattttaccg ggccggccct cctcatgata ttccaaagcg gccgtacgcc
     3001 tgctcgccaa cggatcctcc tgaaaaaaag taggttaccg cgaccgttat ccggacgcaa
     3061 agggcagtcg gcgaggacac agaggcggaa ctgcagggtt cgcagaaggc caccgcgggg
     3121 gaaggggcca tacccggggc cgggcgaaaa cgcgcacgcg ctcgcgcccg gccgttacga
     3181 cgtcagagcg tcgacttgaa caacaggcga ttgggcgtgc ccgacacgtt accggaaatc
     3241 acaccggtcg tggcgttgct gttgatccag ctcacgattg tcgccgagga agcgttgccg
     3301 tatgtgccct tgtaaagcgc ggccaccccg gccacgtgcg gcgacgccat ggatgtaccg
     3361 ctgatggtat tggtcgcgct cgtactgccg atccaggccg aacgaatcga cgagcccggc
     3421 gcgtaaccat caacacagct gccatagttc gagtacgatg ccttggcatc actgctggtc
     3481 gacgccgcag tcgtatacac cgcgctcgcg ctggccggcg aatagttgca cgcattcgcg
     3541 ttgctgttgc ccgccgccac cgcaacgaac accccggagt tgtgcaggtt ggtgaccgcg
     3601 gtgttgagcg aggacgagta gccgccgccc agtgaaagat tcgcgaccgc cgggttgac
//
LOCUS       DASURG010000150         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1356320, whole genome shotgun sequence.
ACCESSION   DASURG010000150 DASURG010000000
VERSION     DASURG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356320"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2673)
                     /gene="smc"
                     /locus_tag="VFY80_02470"
     CDS             complement(<1..2673)
                     /gene="smc"
                     /locus_tag="VFY80_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX6006796.1"
                     /translation="MRLTQINLAGFKSFVDPTRIPVPGQLVGIVGPNGCGKSNVIDAV
                     RWVLGETSARHLRGETMQDVLFNGSGERKPVNRASVELIFDNSLGRAAGQWSQYAEIS
                     IKRVLQRDGDSSYYINNTHVRRRDIADLFLGTGLGSRAYAIIEQGMISRVIEAKPEDL
                     RIFLEEAAGVSKYRERRRETELRLKDTRENLLRVDDIRQELDTRIEHLEAQAEVARRY
                     HGLHTELQTTQSLLWLTRKQEAGAARNRHAREIERVTTELEAETARLREAERRLEEMR
                     GQHFRASDEMHTAQGALYEANAEVARLEQQIAHVRDSRARLEHQIATLGAQLEQSESQ
                     LAAAQSSLAEWRAEKGRADEEVAACAVRVSEESGKLPVAEEAYRATYNRRDELQRALS
                     SAEQTREVEQTNLVHAERLIDQLGQRETRLREEQAALEVPDSGTMENLQQEMQELDRE
                     LQARRGALADMERLLPQLEESLRVRNDALDAAIQNITATEARIAALEQLQDRIARGAE
                     LAGWLAARGLEHAPRLWQGIRIESGWEDALEAVLRERLNGIALEEFEKSAAWLEDAPP
                     GKMTVVGVAETAENAAQPASAECEPLLRYVVCRDARLKAALSEWLHDVFVIADAGSGL
                     RLRGTLPPGAVLVSRAGHVFTRHSVSFHAPDSELHGVLTRQREIEELQETLAGEQEAR
                     SKLETHVSDVERDIDAHKARLGQLREAIESGQQQYHALQMEAVRLSEHAQRLTQRGEQ
                     IATELAENAAQRTAEAARRGAAVDNLARVSAETTALSAQLELATDLYRRAEGVLDLQR
                     SAVQDAQHAHQEASFRLRTADSKIIEIEGSLHSLSQNIASLSASLAGLRAELDLCDEG
                     PWQAQLQQALARRSERAQALSAARDALEGMESQLR"
     gene            2748..3164
                     /gene="queF"
                     /locus_tag="VFY80_02475"
     CDS             2748..3164
                     /gene="queF"
                     /locus_tag="VFY80_02475"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX6006797.1"
                     /translation="MPNRPSKGLETFPNPTPQRDYRIHMEIPEFTCLCPRTGQPDFAT
                     LFLDYVPDRRCVELKSLKLYVWSYRNEGAFHEAVTNRILDDLVKATRPRYMRLTARFY
                     VRGGIFTTVVAEHRSGGWKATRMPELEALPGASSNR"
     gene            3211..4452
                     /gene="dapC"
                     /locus_tag="VFY80_02480"
     CDS             3211..4452
                     /gene="dapC"
                     /locus_tag="VFY80_02480"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX6006798.1"
                     /translation="MVLAAGLRAWTKKKTSVNPNLDRLESYPFQRLRALLEGASPPAG
                     LRPIPLYIGEPKHPTPEFIKRTLIEHLDGLAAYPLTAGTEALRAAIGGWLERRYGIPP
                     VDPATQIIPVNGTREALFAFAQAVIDAKRASPVVVCPNPFYQIYEGAALLANATPRFL
                     NSVPERNYAFDPGEIAPDVWERTQLLYVCSPGNPTGHVLTLEEWRDLFGLSERYGFVI
                     AADECYSEIYFDERKPTLGALEAAQKLGWKGYRNLVVFSSLSKRSNVPGMRSGFVAGD
                     ADILKKFLLYRTYHGCAMSLPVQHASVAAWGDERHVAENRLLYRRKFEAALDILKPVT
                     RVDMPDAAFYLWLATPISDTQFTRRLYEAQAVSVLPGSYLAREAHGINPGENRVRIAL
                     VAETDECAEAARRIRDFISGL"
     gene            4464..5285
                     /gene="dapD"
                     /locus_tag="VFY80_02485"
     CDS             4464..5285
                     /gene="dapD"
                     /locus_tag="VFY80_02485"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX6006799.1"
                     /translation="MQKPQTIIERAWEERASLTATNAGLAVKQAVEDTLAQLDEGRLR
                     VAEKQDGAWVTHQWVKKAVLLSFRLQDNVVMHAGDLRYYDKVPSKFTHYSAQQFERGG
                     FRVVPPAAARRGAHIAKNVVLMPSFVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEEGAVISMGVFIGASTRIYDRQ
                     SGKVLYGRVPAGSVVVPGSLPSADGKCSLYCAVIVKQVDAGTRAKTSINELLRSD"
BASE COUNT          894 a         1770 c         1673 g          985 t
ORIGIN      
        1 gcgcagctgg gattccatcc cttccagcgc gtcgcgcgcc gcactgagcg cctgcgcgcg
       61 ctcgctcctg cgggccaacg cctgctgcag ctgcgcctgc cacggcccct cgtcacacag
      121 gtcgagctcg gcgcgcagcc ccgcgaggct cgcgctcagg gaggcgatgt tttgagaaag
      181 agaatgaagt gagccctcta tctctatgat tttgctatct gccgtcctga gcctgaagga
      241 agcctcttgg tgtgcgtgct gggcgtcctg cacagccgag cgctgcaggt ccagaacacc
      301 ctcggcacgc cggtaaaggt cagtcgcgag ctcgagctgc gcggataatg ccgtcgtttc
      361 tgccgaaacg cgggcgaggt tatcgactgc agccccccgg cgcgccgctt cagccgtccg
      421 ctgcgccgcg ttttccgcga gctcagtggc gatctgctcg ccgcgctggg tcaggcgctg
      481 cgcgtgctcc gatagccgaa cggcctccat ttgaagcgcg tgatactgct gctggcccga
      541 ctcgatcgct tcacgcagtt gccccagcct tgccttgtgc gcgtcgatat cgcgctcgac
      601 gtcgctcacg tgtgtctcga gttttgagcg cgcctcttgc tcgcctgcca gcgtctcctg
      661 cagctcctcg atctcgcgct ggcgcgtgag cacgccgtgc agctcggaat cgggagcatg
      721 gaagctcacg ctgtgccgcg tgaacacatg ccccgcgcgc gaaacgagca ccgcgccggg
      781 cggcagagtc ccgcgcaggc ggagacccga tcccgcgtcc gcaatcacga acacatcgtg
      841 cagccattcg ctcaaggccg ctttgagccg cgcgtcgcga cagacgacgt aacgcagcag
      901 cggctcgcat tcggccgatg ccggctgtgc cgcgttctca gcggtttccg ccacaccgac
      961 gaccgtcatt ttccccgggg gggcgtcctc gagccatgcc gcgctctttt caaattcctc
     1021 gagcgcgata ccattgagtc gctcgcgcag caccgcttca agtgcatctt cccagccgga
     1081 ttcaatccgt atgccctgcc acaaccgtgg cgcgtgctcc aggccacgcg cagcgagcca
     1141 gccggcaagc tcggcgccgc gcgcgatgcg gtcctgcaat tgctcgagcg ccgcaatgcg
     1201 cgcctcggtg gcggtgatgt tctggatcgc cgcgtcgagc gcatcgttgc ggacgcgcag
     1261 actctcttcc agctgcggca gaagccgttc catgtccgcc aaagcgccgc gtcgcgcctg
     1321 aagctcccgg tcgagctcct gcatctcctg ctgcagattc tccatcgtcc cagaatcggg
     1381 cacctcgagc gctgcctgtt cctcacgcaa acgggtctcg cgctgcccga gctgatcgat
     1441 caggcgctcg gcatggacga ggttcgtctg ctccacctcc cgcgtctgct cggcgctcga
     1501 aagggcgcgc tgcagctcat cgcgacggtt gtaggtcgca cgataggctt cttccgcgac
     1561 cggaagtttt ccgctttctt ccgacacgcg cacggcacag gccgccactt cctcatcggc
     1621 acgccccttt tcggcgcgcc attcggcgag gctggactgc gcagcggcaa gctgtgactc
     1681 gctctgctcg agctgcgccc cgagcgtcgc gatctggtgc tcgagacgcg cgcggctgtc
     1741 gcgcacgtgc gcgatctgct gctcgagacg cgcgacctcc gcgttggctt cgtacagcgc
     1801 accctgcgcg gtgtgcatct catcgctcgc gcggaaatgc tggccacgca tttcctcgag
     1861 ccgcctttcg gcctcgcgga gcctggcggt ttccgcttcg agctcggtgg tgacgcgctc
     1921 aatctcccgc gcatggcggt tgcgggcggc gcccgcttcc tgcttgcgag tgagccagag
     1981 caggctctgg gtcgtctgca gctcggtgtg cagcccgtgg tagcgccgcg cgacctcggc
     2041 ctgagcttcg agatgctcga ttcgcgtgtc gagttcctgc cggatgtcgt ccaccctcag
     2101 caggttctcg cgcgtatcct tgagccgcag ctccgtctcg cgccgccgct cccggtactt
     2161 cgacacgccg gccgcctctt cgaggaagat gcgcaggtct tccggtttcg cttcgataac
     2221 gcgcgaaatc atgccctgct cgatgatggc gtaggcgcgg ctgcccaggc cggtgccgag
     2281 gaagagatcg gcgatgtccc ggcgccgcac gtgggtgttg ttgatgtagt aggaggaatc
     2341 gccgtcgcgc tgcaggacgc gcttgatgga gatctctgcg tactgcgacc actggcccgc
     2401 cgcccggccg aggctgttat cgaagatcag ctcgacactg gcgcgattga ccggcttgcg
     2461 ctcgcccgaa ccgttgaaca gcacgtcctg catcgtctcg ccgcgcaagt ggcgcgcgga
     2521 cgtctcaccc aggacccagc gaacggcatc gatcacgttc gatttgccgc aaccgttggg
     2581 gccaacgatg ccgacgagct gtccggggac gggaatacgg gtgggatcga cgaacgactt
     2641 gaaaccggcg aggttgatct gcgtgaggcg caatgtgttc tacagcaatg agtttataat
     2701 ttcggcgcga ttgccgggcc attttaacca attttcaccg ttacgctatg ccgaacaggc
     2761 cgagcaaggg cctcgaaacg tttcccaatc cgacgccgca gcgggattac cggattcaca
     2821 tggaaatacc ggaatttacc tgcctgtgtc cgcggacggg tcagcccgac ttcgccacgc
     2881 tctttctcga ctacgtgccc gatcgccgct gcgttgagtt gaaaagtctc aagctctacg
     2941 tctggtcgta tcggaacgag ggcgcgtttc acgaggccgt gacgaatcgg atcctcgacg
     3001 atctcgtcaa ggcgacacgc ccgcgctaca tgcgtctcac ggcgcgcttc tacgtgcgcg
     3061 gcggcatctt caccaccgta gtggctgaac accgatccgg cggctggaaa gcgacccgca
     3121 tgccggagct cgaggcgctg ccgggcgcat cgagcaaccg ttgacgctcg gacggatttt
     3181 gaacattccc tataatcgga cggcgccaac atggtccttg ccgcaggtct tcgcgcttgg
     3241 accaagaaga agacttccgt gaatccgaac ctcgaccggc tggagagcta tccttttcag
     3301 aggctgcgcg cgctgctcga aggcgcgagc cccccagccg gcttgaggcc gataccgctc
     3361 tatatcggcg aacccaagca tccgacgccg gagttcatca agcgcaccct gatcgaacac
     3421 ctggacggtc tcgccgccta ccccctcacc gcaggtacgg aagcgttgcg cgctgccatc
     3481 ggtggttggc tcgagcgccg ctacggcatt cccccggtag atccggcgac gcagatcatt
     3541 ccggtaaacg gcacccgcga ggcgctcttc gccttcgcgc aggccgtcat cgatgccaag
     3601 cgtgcgagcc ccgtcgtggt ttgcccgaat ccgttctatc aaatctacga gggcgcggcg
     3661 cttctcgcga atgccacacc ccgcttcctg aacagcgtgc cggagcgcaa ctacgcattc
     3721 gatccgggcg agatcgctcc ggacgtgtgg gagagaacgc aactgctcta cgtctgctcg
     3781 ccgggcaacc cgaccgggca cgtgctcacg cttgaagagt ggcgggacct cttcgggctt
     3841 tccgagcgtt acggtttcgt gatcgcggcc gacgaatgct attccgagat ctacttcgac
     3901 gagcgcaagc ctacgctcgg cgccctcgag gcggcgcaga agctcggctg gaagggttac
     3961 cgcaatcttg tcgtgttctc gagcttgtcg aaacgatcga acgtgcccgg catgcgatcc
     4021 ggtttcgtcg cgggcgatgc ggacatattg aagaaatttc tgctctatcg cacgtatcac
     4081 ggctgcgcga tgagcctgcc agtccagcac gcgagcgttg cggcatgggg ggacgagcgt
     4141 cacgtggcgg aaaaccgcct actctatcgg aggaagttcg aagccgcgct ggacatattg
     4201 aagcctgtga ctcgagtgga catgccggac gcggccttct acctctggct cgcaacgccg
     4261 atcagcgaca cgcaattcac gcgccgcctg tacgaggcgc aggcggtctc cgtcctgccg
     4321 ggaagttacc ttgcgcgcga ggcgcacggc atcaacccgg gcgagaatcg cgtacgcatc
     4381 gccctggttg cggaaaccga cgaatgcgcg gaggcggcgc gccgcatacg cgacttcatt
     4441 tccggactct gatccgaggg attatgcaga aaccgcagac gatcatagaa cgcgcgtggg
     4501 aagagcgcgc gtcgcttacc gcgacgaacg ccgggctcgc cgtgaaacag gccgtggaag
     4561 acaccctggc gcagctcgac gaaggccggc tgcgcgtggc ggaaaagcag gacggcgcgt
     4621 gggtgacgca ccagtgggtc aagaaagcag tactgctgtc gttccgcctg caggacaacg
     4681 tcgtcatgca cgcgggcgac ctgcgctact acgacaaggt gccctcgaag ttcacgcact
     4741 acagtgccca gcagttcgag cgcggcgggt tccgggtcgt accccctgct gccgcccggc
     4801 gcggcgcgca catcgccaag aacgtggtgc tcatgccgtc gttcgtcaac atcggcgcat
     4861 acgtcgatga gggaacgatg gtggatacct gggcgaccgt gggctcgtgc gcgcagatcg
     4921 gaaagaacgt gcacctctcc ggcggggtcg gcatcggcgg cgtgctggag ccgctgcagg
     4981 cgaacccgac catcatcgag gacaattgct tcatcggcgc gcgctcggaa atcgtcgaag
     5041 gcgtgatcgt ggaagaaggc gccgtcattt cgatgggcgt cttcatcggc gcgagcaccc
     5101 gcatctacga tcgacagtcg gggaaggtgc tctacgggcg cgttccggcc ggttccgtgg
     5161 tggttcccgg ctcgcttcct tccgcggacg gcaagtgcag tctctattgc gccgtaatcg
     5221 tgaagcaggt ggacgccgga acccgggcca agaccagtat caacgagctg ctgcgcagcg
     5281 actgatactc tggagtgcag cagccgcgca agaaaacgct cc
//
LOCUS       DASURG010000151         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1356418, whole genome shotgun sequence.
ACCESSION   DASURG010000151 DASURG010000000
VERSION     DASURG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356418"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFY80_02490"
     CDS             <1..537
                     /locus_tag="VFY80_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810362.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX6006800.1"
                     /translation="VRSGFDHAVARGLAYAPYADLIWCETATPDLAFARRYAEAIRRT
                     YPDKMLAYNCSPSFNWRRNLDASDISKFQRELGAMGYKFQFVTLAGFHALNYSMFELA
                     KGYAHEHMSAYVRLQDAEFAAEELGYTATRHQHEVGAGYFDAVTQVIQGRHAATTALS
                     GSTEQAQFRDVPLSEIAA"
     gene            561..2210
                     /gene="aceB"
                     /locus_tag="VFY80_02495"
     CDS             561..2210
                     /gene="aceB"
                     /locus_tag="VFY80_02495"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX6006801.1"
                     /translation="MELNTILPPGVEVRGRMTSQYAEILTPAALAFLVALARAFEPRR
                     RELLAARAHRQAAFDAGELPDFLPQTAHIRNAGWKVEAAPADLQDRRVEITGPTDRKM
                     VINALNSGANVYMADFEDSNVPTWENMVEGQINLRDAVNRTIAFTGSDGRRYRLSAST
                     ATLLVRPRGWHLPEMHVLIDGEPVSAAIFDFALYFFHNAKTLLASGTGPYFYLPKIES
                     HLEARLWNDIFIAAQSALGIARGTIKATVLIETVLAAFEMDEILYEMREHCVGLNIGR
                     WDYLFSCIKKFRNRHDFCLADRAHVTMTAPFMRAYALLLVKTCHRRGAFAMGGMAAQI
                     PIKNDAAANAAALDKVRADKLREVNDGCDGTWVAHPGLVHIAREVFDTHMPTPNQIDR
                     QRDDVQVTAAELLDFRPERPITEAGLRNNISVGIQYLGAWLAGNGCVPVYNLMEDAAT
                     AEISRSQIWQWIRSSKGVLEDGRKVTEPLVCQMIDDELPRIAHALGKANWRAGRYEAA
                     ARLFGDLVTCDDYAEFLTLPAYDLIACEPAAEQTKDAALAV"
BASE COUNT          438 a          741 c          747 g          418 t
ORIGIN      
        1 gtgcgttcgg gcttcgatca cgcagtggcg cgcggcctcg cttatgcgcc ttacgccgat
       61 ctcatctggt gcgaaaccgc cacgcccgac ctcgcgttcg cacgccgcta tgccgaggcg
      121 atccgtagga cgtatcccga caagatgctg gcatacaact gctcgccgtc gttcaactgg
      181 cgcaggaacc tcgatgcgtc cgacatatcc aagtttcagc gggaacttgg cgccatgggc
      241 tacaagttcc agttcgtgac actggccggc ttccatgcgc tcaactacag catgttcgag
      301 ctggccaaag gctacgcgca cgagcacatg agcgcgtatg tccgtctgca ggacgccgag
      361 tttgccgccg aggaattggg ttacacggcg acgcggcacc agcacgaggt cggcgcgggt
      421 tatttcgatg cggtgacgca ggtgatccag gggcgtcacg ctgcgacgac cgcgctttcc
      481 ggctcgaccg agcaagcgca gttccgcgac gtccctttgt cggaaatcgc cgcttgaaag
      541 cgaaacttca aggagcacgc atggagctca acaccatcct tcccccgggc gtcgaagtgc
      601 gcggccgcat gacgtcccaa tatgccgaga tcctcactcc ggcagcgctt gcgttcctcg
      661 tggcgctcgc gcgcgcgttc gagccgcgcc ggcgcgagct gcttgcggca cgcgcgcacc
      721 gtcaggccgc attcgacgcg ggcgaactgc cggatttcct tccgcagacc gcgcatatcc
      781 ggaacgcggg ctggaaggtc gaggccgcgc cagcggactt acaggatcgg cgcgtcgaga
      841 tcaccggacc gacggatcgc aagatggtga tcaatgcgct caactcgggc gcgaacgtgt
      901 acatggccga tttcgaagat tcgaacgtgc ccacgtggga gaacatggtc gaggggcaga
      961 tcaacctgcg cgatgcggtg aaccgtacca tagcgttcac cgggagtgac gggcggcgct
     1021 atcggctgag cgcgagcacc gccacgctgc tcgtgcgacc gcgcgggtgg catctgcccg
     1081 aaatgcacgt gctcatcgac ggcgagccgg tgtcggcggc gatctttgat ttcgcgcttt
     1141 atttctttca caacgcgaag acgctactcg ccagcggaac gggcccgtac ttctacctgc
     1201 cgaagatcga aagccacctc gaagcgcggc tgtggaacga catcttcata gcggcccaga
     1261 gcgcgctcgg cattgcgcgc ggtacgatca aggcgaccgt actcatcgaa acggtcctcg
     1321 ccgccttcga gatggacgag atcctctacg agatgcgcga gcactgcgtc gggctcaata
     1381 tcgggcgctg ggattacctc ttcagctgca tcaagaaatt caggaaccgc cacgacttct
     1441 gtctcgcaga tcgcgctcac gtgacgatga ccgcaccctt catgcgggct tatgcgctcc
     1501 ttctcgtgaa gacctgtcac cgccgcggcg ccttcgcgat gggcggaatg gccgcacaga
     1561 tcccgatcaa gaacgatgcg gccgcgaacg cagcagcgct cgacaaggtg cgcgccgaca
     1621 agctgcgcga agtgaatgac gggtgtgatg gcacgtgggt tgcgcacccc gggctggtgc
     1681 acatcgcgag ggaggtgttc gatacgcata tgccgacgcc aaaccagatt gaccgtcagc
     1741 gcgatgatgt ccaggtcact gctgcggagc tgctcgactt ccgtccggag cgtccgatca
     1801 cggaagcggg cttgcggaac aacatcagcg tcggcatcca gtacctcggc gcgtggcttg
     1861 ccggcaatgg ctgcgtgccg gtttacaacc tcatggagga tgctgcgacg gcagaaattt
     1921 cgcgctctca gatctggcag tggatacgca gctccaaggg cgtgctggag gacggccgga
     1981 aagtgacgga gccgctcgtc tgccagatga tcgacgacga gctgccgcgc atcgcgcacg
     2041 ctctcggtaa ggcgaactgg cgcgccgggc gctatgaagc cgcggcacgc ctcttcggtg
     2101 atctggtcac ctgcgatgac tatgccgagt ttctgaccct gcccgcgtac gatctcatcg
     2161 cgtgtgaacc ggcggccgag caaacgaaag acgccgcatt ggcggtgtag cgcggttagt
     2221 gcagggaggt tcccggcgtc ggggattcca gaagccgttt cacgctcgcg gtgtcgagcg
     2281 gcttcaccat gtgcaggtcg aagcccgcct ccttagactc gcggacgtcc gacggctgtc
     2341 ccca
//
LOCUS       DASURG010000152         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_642240, whole genome shotgun sequence.
ACCESSION   DASURG010000152 DASURG010000000
VERSION     DASURG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642240"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFY80_02500"
     CDS             complement(<1..722)
                     /locus_tag="VFY80_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006802.1"
                     /translation="MLKACAGSLRLEFRETKAGTPVKSVESIREHVLNGIALNREPGY
                     HFAGNFLDISFDHIVARDSRLSMESGPHCIEPDGQMDLGAFAVFADLALAATVRANVS
                     PETRLATVYMNLHFTGARIAGRLEAAASFENFFHGAAGRQGLGRVRIAADGEQVCIGH
                     GAFMVLKPPKGVALHPMPQRRRDDPLPSPVAGKLQRDEAKILALTDAALGSVDARGGS
                     FIRRFWGYEPHGVEGGASCVVK"
     gene            805..1692
                     /locus_tag="VFY80_02505"
     CDS             805..1692
                     /locus_tag="VFY80_02505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6006803.1"
                     /translation="MARAGIIGCGLMGTACARRLIGATFDVYAYDVSATQLDAIATIG
                     ARPIRTLAELVASCETLIVAVFNTDQVEAVVAGPDSVQDLIRATKHARTIICVSTCDP
                     DRLAALAERVAADGVRFLEVPISGTSRQVAQGEGVGLIGGDRSVMAEAAGVLDAICPR
                     RYYLGAVGNGSRAKLAVNLILGLNRAALAEGLVFAERLGLDRSAFLEVARGSAAYSQV
                     MDVKGPLMARREYRHPQSRVDQSLKDFRLMIEQAQARGQDLPFASLYAWMLEDCMKHG
                     EAEWDNAAIAEAIGRRYET"
     gene            1708..2676
                     /locus_tag="VFY80_02510"
     CDS             1708..2676
                     /locus_tag="VFY80_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006804.1"
                     /translation="MHIVIAAVIALAGVAPYGEAHAQTYPAKPIRLIVPFPAGGGADI
                     FARLIGRKLSDNMRQSIVVDNRAGASGIIGCEAVARSAADGYTLLMGTTGTHTTNPAV
                     FSKLPYDPLKDFTPISLVADSPFVLLVHPSLPVANLKELIAFAKARPGQLTYASSGTG
                     SSSHLGFELFNLMAGIKGVHVPYKGLPPATLDTISGYVTMTWNSITAAAPYLKNKQLK
                     ALGIGSVKRSALMPDIPTISEAGLPGFELGSWYGIFAPAGTAPEIVRRIQSEIVKAIN
                     DPGMNDQFAALSAEPVGSTPDEFRAVLQRDLVKWAKVAKQANVKAD"
     gene            complement(2698..3045)
                     /locus_tag="VFY80_02515"
     CDS             complement(2698..3045)
                     /locus_tag="VFY80_02515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX6006805.1"
                     /translation="MERPHPDRPRRFQVSRRGQLSYEEHQFACLVQDISLKGLFVICN
                     YDLEIGLELMIRFELEPGLPFEGKIKVRHFHDGCFGAELVEAEPSYLDNWNQFLIDEF
                     GDQTTLPERRARR"
     gene            complement(3060..4220)
                     /locus_tag="VFY80_02520"
     CDS             complement(3060..4220)
                     /locus_tag="VFY80_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6006806.1"
                     /translation="MNEPLTGRTLALRETADVPLRIKRIDPIPVALPLTKPVVMAGER
                     VEYAYNLIVRVEAENGLVGWGEAASAPLMTGDVLPGMVAAVNDYLAPLVKGEDALQRA
                     RLAQRCARALLHNTGAKCAVDMAINDLVGRYLNVGLADLFGGALRETLQPMYLLGNPK
                     VEDDIAEARRKLEEGWSFFKLKIGIKPALDEARAANEIRRALGNDVALCADANMGMTA
                     ADARAFAQNAATAKLLFMEQPLHSDDVEGMTALARSSPIPINGDESIASVASLVDLAR
                     RGAIQGANIKTIKMGGVTEAVRAMSVCGALGLSINLAGKVAESSIAAAAIVQLGCLAP
                     NLDWGLNITNHYLAEDVTDAPLPIERGLVHRPRAPGLGITVSEARVNRFRMK"
     gene            complement(4276..>4591)
                     /locus_tag="VFY80_02525"
     CDS             complement(4276..>4591)
                     /locus_tag="VFY80_02525"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX6006807.1"
                     /translation="EAALRAYALAGIPRESARAMMKPLLRETADNALGMGTVSALTGP
                     IARGDAALVAQQLDALEKADPSLAALYRELGTIAVELARAQQKAHAAALDAIQEILTR
                     DG"
BASE COUNT          810 a         1484 c         1447 g          850 t
ORIGIN      
        1 ttcttcacca cgcaggaagc gccgccctcg acgccgtgcg gttcgtatcc ccagaagcgg
       61 cggatgaacg atccgccgcg cgcatcgacg gagccgagcg cggcgtccgt gagcgccaag
      121 atcttcgcct cgtcgcgctg cagtttgcct gcgacggggg atggcagagg atcatcgcgg
      181 cggcgctgcg gcatggggtg cagcgcgacg cctttcggcg gcttcaacac catgaacgcg
      241 ccgtggccga tgcacacctg ctccccgtcc gccgcgattc tcacgcggcc caggccctgc
      301 cgtcctgccg cgccgtggaa gaaattctcg aagctcgcag ccgcttcaag ccgcccggca
      361 atgcgcgcgc cggtgaagtg cagattcatg tataccgttg caaggcgcgt ttcgggcgag
      421 acattggcgc gcacggtcgc cgcgagcgca aggtccgcga agacggcgaa agcgcccaga
      481 tccatttgcc cgtccggctc gatgcaatgc ggaccgctct ccatggacag tcgtgaatcg
      541 cgggccacga tgtggtcgaa cgagatatcg aggaagttgc cggcgaagtg atagccgggc
      601 tcacgattga gtgcgatgcc gttgaggacg tgctcgcgga tcgattcgac gctcttcaca
      661 ggcgttccgg ccttcgtttc cctgaattct aaccgcagtg accccgcgca cgccttcagc
      721 atgccgataa tgcaaccggg cagaacgggt atactcgcac gcgatctggc tccggcagtt
      781 cacatcttgg aggatgacgc gtctgtggca cgggcaggca tcatcggctg cggattgatg
      841 ggcacggcgt gcgcgcggcg gctcatcggc gcgacattcg acgtgtacgc ctatgacgta
      901 agcgccactc agctcgacgc catcgcgacc atcggcgcgc gaccgattcg cactctcgct
      961 gaattggtcg cgtcatgcga aacgctgatc gtcgccgtgt tcaacaccga ccaggtggaa
     1021 gcggtcgttg cggggccgga cagcgtgcag gatcttatac gcgccacgaa acacgcgcgc
     1081 accatcatct gtgtaagcac ctgcgatccc gatcgtctgg ctgcgctcgc agaacgcgta
     1141 gcggccgatg gcgtgcgttt tctcgaagtg ccgatctcgg gcacaagccg gcaggtcgcc
     1201 caaggtgaag gtgtgggact gatcggcgga gatcgctcgg taatggccga ggcagcaggg
     1261 gttctggatg ccatctgccc gcggcgctac taccttggcg cagtgggcaa cggctcgcgc
     1321 gccaagctcg ccgtcaatct gatcctcggc ctcaatcgcg ccgcactggc ggaagggctc
     1381 gtctttgcgg agcgtctggg gctcgatcgc tcggcgtttc tcgaagtcgc gcgcggctcc
     1441 gccgcctatt cccaggtgat ggacgtgaag ggtcccctca tggcccggcg ggaataccgt
     1501 catccgcaaa gccgcgtcga tcagagcctc aaggatttcc gcctgatgat cgagcaggcg
     1561 caggcgcgcg gtcaggattt gccctttgca agcctgtacg cgtggatgct ggaggactgc
     1621 atgaagcatg gtgaggcgga atgggacaat gcagccatcg cagaagcgat tggccgtcgc
     1681 tatgagacat gaactaggaa acctgccatg cacatcgtca tcgctgcggt tatcgcgctt
     1741 gccggagtcg ctccgtacgg ggaggctcat gcacaaacct atcccgcaaa gcccatccgg
     1801 ctgatcgtgc ccttcccggc gggcggcggc gccgacatct ttgcgcggtt gatcggtcgc
     1861 aagctgagcg acaacatgag acagagcatc gtggttgaca accgcgccgg cgcatccggg
     1921 atcatcggct gcgaagccgt tgcgcgctcg gcggcggacg gctataccct gctcatggga
     1981 acgaccggca cgcacacgac gaaccctgcc gttttctcca agctcccgta cgatccgctc
     2041 aaggacttca cgccgatttc gctcgtcgcc gattcaccgt tcgtgctgct cgttcatcct
     2101 tcgctgccag tcgcgaacct caaggagttg atcgcgttcg ccaaagcgcg gccgggtcag
     2161 ctcacctatg cgtcttcagg gaccgggagc tcatcgcatt tgggcttcga gctgttcaat
     2221 ctgatggcag gcatcaaggg cgtgcacgtc ccgtacaagg gcttaccgcc cgcaacgctc
     2281 gatacgatct ccggttacgt caccatgacc tggaattcga tcacggccgc tgcaccctat
     2341 ctcaagaata agcaactgaa ggccctcggt atcggctcgg tgaagcgctc cgcgctgatg
     2401 cccgacattc cgaccatttc ggaagccgga ctgccaggct tcgagctcgg ttcatggtac
     2461 ggcattttcg cgccggccgg caccgcaccg gagatcgtgc gccggattca gagcgaaatc
     2521 gtaaaggcga tcaacgaccc cggcatgaat gaccagttcg cggcgttgag cgcggagccg
     2581 gttggcagca cgccggacga atttcgtgcg gtcctgcagc gcgacctcgt gaaatgggcg
     2641 aaagttgcaa agcaggcgaa cgttaaagcg gattagccaa cactgcctgc tcgggcgcta
     2701 gcgccgcgcg cgacgctcgg gaagggtcgt ctggtcgccg aactcgtcta tcaggaactg
     2761 attccagttg tcgagatagc tgggctcggc ctcgacgagc tccgcgccga agcatccgtc
     2821 atggaaatgc ctcaccttga tcttcccctc gaagggcagg ccgggctcga gctcgaaccg
     2881 gatcatcagc tcgaggccga tttcgagatc gtagttgcag atcacgaaaa gccctttgag
     2941 gctgatgtcc tggacgaggc aggcgaactg gtgctcttcg tacgacaact gaccgcgccg
     3001 gctgacctga aagcggcgcg gccggtcggg atgcggtctc tccataatac ccttcctgcc
     3061 tacttcatcc ggaaacgatt cacacgcgct tcgctcaccg tgatgccgag acccggcgcg
     3121 cgcggccggt gcacgaggcc gcgttcgatc gggagcggcg catcggtcac gtcctcggcg
     3181 aggtagtgat tcgtgatgtt gaggccccag tcgaggttgg gcgcgaggca gccgagttga
     3241 acgattgcag cggccgcgat gcttgattcg gccacctttc cggcaagatt gatggagagg
     3301 ccaagcgcgc cgcacacgga catcgcccga accgcctcgg ttacaccgcc catcttgata
     3361 gtcttgatgt tggcgccctg gatcgcaccg cggcgcgcga gatcgacgag gctcgcgacg
     3421 ctcgcgatcg attcgtcgcc gttgatcggg atcgggctcg accgcgcgag cgccgtcatg
     3481 ccttccacgt catcgctgtg gagcggctgc tccatgaaaa gcagcttggc cgtcgccgca
     3541 ttctgggcaa aggcccgggc atcggctgcc gtcatgccca tgttcgcatc cgcacaaagc
     3601 gcaacgtcgt tgcccagcgc gcgccgtatt tcgttcgcag cgcgggcctc atcgagcgct
     3661 ggcttgatgc cgatcttcag cttgaagaac gaccaccctt cttccagctt gcgcctcgct
     3721 tctgcgatat cgtcttccac tttgggattg cccagcagat acatcggttg cagcgtctcg
     3781 cgcagcgcgc cgccgaagag atcggcgagc cccacgttca gatagcggcc gacgagatcg
     3841 ttgatcgcca tatcgaccgc acatttcgcg ccggtgttgt gcaggagtgc ccgcgcgcag
     3901 cgctgcgcca ggcgcgcgcg ctgcagcgcg tcctcgccct tgacgagcgg agcgagataa
     3961 tcgttgacgg cggctaccat gccgggcaag acatcgcctg tcatgagcgg tgcggaagcc
     4021 gcctcacccc agccgacgag tccgttttcc gcctcgaccc gcacaataag gttgtaggcg
     4081 tactcgacgc gctcgccggc catgacaacc ggcttcgtca gcggcaaagc gaccggaatc
     4141 ggatcgatac gcttgatcct gagcggcacg tcagcggttt cccgcaacgc caaggtcctg
     4201 cctgtcagcg gctcattcat agcctctccg gtcgcattcg gcgcattcta acgcctgccg
     4261 gtttggcacg gcactttagc cgtcacgtgt gaggatttcc tgaatggcgt cgagcgccgc
     4321 ggcgtgcgcc ttctgctgcg cccgggcaag ctcgacggcg atcgtgccca attcccggta
     4381 gagggcggca agggacggat cggccttttc caatgcgtcg agttgctggg cgaccagcgc
     4441 agcatccccc cgcgcgatcg ggcccgtgag cgcgctcacc gtgcccattc ccagcgcgtt
     4501 gtcggccgtt tcgcgcagaa gcggcttcat cattgcccgc gcgctctcgc gtgggatgcc
     4561 ggcgagcgca taagcgcgca gcgcagcttc c
//
LOCUS       DASURG010000153         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1459643, whole genome shotgun sequence.
ACCESSION   DASURG010000153 DASURG010000000
VERSION     DASURG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459643"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFY80_02530"
     CDS             <1..194
                     /locus_tag="VFY80_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006808.1"
                     /translation="LAEALRRIAPYLLTELLLPGGTLLALVLFVSRYRRKRRAQMAAA
                     AANDAPVPHPETAAVARAA"
     gene            complement(205..1044)
                     /locus_tag="VFY80_02535"
     CDS             complement(205..1044)
                     /locus_tag="VFY80_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX6006809.1"
                     /translation="MPEARPLRDRLALHHWTLEPLPLAEFLRVARDTGWNAVELRRSS
                     FTKCFEAGMSNAQVVDLIRQSGIRLAVLGTEYGLYFARGEEQRRLLKVLDETCANAVA
                     LGCDTIMCAEGHGSGTVREAGANIRAAGDICARHNVRFAFEFSSAAQVVNHLDVAREV
                     LALASHPACGLLLDAYHLERTGCGGRAFADVPERDIFAFQYSDVPITPRAPTGRPTDR
                     LPPGRGIVRWREVLTLLTEKNYSGYLSYEAPNPDHWSGLPAEVARAGLEATRARIAEI
                     EPL"
     gene            complement(1037..2203)
                     /locus_tag="VFY80_02540"
     CDS             complement(1037..2203)
                     /locus_tag="VFY80_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6006810.1"
                     /translation="MAKEPAYFMYFPGNYRWSAAFINMMGSIAYGGAEIGELHKIGRL
                     LSGKAPDDDAAWFDACVKVADGVRGYAEKWDKSGHRYAAAHAYLRACNYYQMAERFRT
                     PKDKTGLEAYRRGVDCFHRHVALTDLKIEIVEVPFADGSLPGYFVHAQNAKSARAPCV
                     VFFDGLDVTKEIQFVRGVPDLVKRGISVLVMDGPGTGEAIRFRGHYLRHDYEVAGSAC
                     VDYLEKRADVDPRKIGIVAISLGGYYSPRCAAMEPRYAACIAWGAQWDYHDTWKKRIE
                     AQFKASLSVPGHHIMWILGVDSLDAALKKLEPFRLDGVMQKMRCPFLLVHGADDEQIP
                     LADAQKQFDACGSKDKTFRVYTAEEGGAQHCQRDYLTLVVAEMWNWFEEKLVRA"
     gene            complement(2271..3236)
                     /locus_tag="VFY80_02545"
     CDS             complement(2271..3236)
                     /locus_tag="VFY80_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006811.1"
                     /translation="MRVASLIAGLLMPLAASHALAQSYPTKPIRIIVPFAAGGPTDTH
                     SRWAGQQLTAAFGQQVIVENRGGAGGVIGTEAVAKAAPDGYTLLGGNPGPLTIARSVR
                     EKLNYDGLRDFAPITLIARSASCMCAHPSVPAKGVKDFIAIAKRSPGKINYATPGVGT
                     VGHFAIEHFAAVAGIKMTMVPYRGAALYVVDLIAGNIDYAQVQIFQARPYVRDGKMRA
                     LGVTAIKRSPLLPEVPTAAEQGLKGWSSYNWNGILAPAGTPQAIVDRIHAVLSKPLND
                     PATRKQLEDIGYEVSGIGPAEYAAFLKEETEKWADVAKKAGIPKI"
     gene            3394..>3794
                     /locus_tag="VFY80_02550"
     CDS             3394..>3794
                     /locus_tag="VFY80_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6006812.1"
                     /translation="MAAKKARAAARKYRDILYEVKDQVAWVTINRPRVLNAFREHTLD
                     EMIDALKSTREDPSIVCAVVTGAGDKAFSAGGDFYAMKRLNFANAYMWNDRMLGLAMT
                     IRGLPIPVIAMVNGWCMGGGHELALWCDVVIA"
BASE COUNT          613 a         1230 c         1237 g          714 t
ORIGIN      
        1 cgctcgccga ggcgttgcgg cgtatcgcgc cttacctcct gaccgagctg ctgctccccg
       61 ggggaacgct gctggcgctc gtccttttcg tgtcgcgcta ccgccggaaa cgtcgcgcgc
      121 agatggccgc ggcggcggcg aacgatgcac ccgttccgca tccggaaacc gcagcggtcg
      181 cacgggcggc ctagctcgag cgcttcagag cggttcgatt tccgcgatcc gcgcgcgcgt
      241 cgcctcgagg cccgcgcgtg caacttccgc gggcagcccc gaccaatgat ccggattggg
      301 cgcctcgtaa ctcaggtagc cggagtagtt cttttcggtg agcagggtca ggacctcgcg
      361 ccagcgcacg atgccgcgtc ccggcggcag gcggtcggtc gggcgtccgg ttggcgcgcg
      421 cggcgtgatc ggcacatcgc tgtactggaa ggcgaaaatg tcgcgttccg gcacgtctgc
      481 aaaggcgcgt ccgccgcagc ccgtgcgctc gagatgatag gcgtcgagca acaggccaca
      541 cgccggatgg ctggcaagcg ccagcacttc gcgggcgacg tcgagatgat tgaccacctg
      601 cgcggccgag ctgaactcga aggcgaagcg cacgttgtgc cgggcgcaga tgtcccccgc
      661 agcgcggatg ttggcgcccg cctcgcgcac ggtgccactg ccgtggcctt cggcgcacat
      721 gatcgtgtcg cagccgagtg ccactgcgtt ggcgcacgtc tcgtcgagca ctttcagcag
      781 ccggcgttgt tcttcccctc tcgcaaagta aagcccgtat tcggttccga ggacggcaag
      841 ccggattccg ctctgccgga tcaaatcgac gacctgcgca ttgctcattc ccgcctcgaa
      901 gcacttcgtg aaactgctgc gacgcagctc cacggcgttc cagccggtgt cgcgtgcaac
      961 ccgcaggaat tcggcgagcg gcaagggctc gagcgtccag tgatggagcg cgagccgatc
     1021 gcgcaagggg cgtgcctcag gcacggacga gtttctcctc gaaccagttc cacatctcgg
     1081 cgacgaccag cgtgaggtag tcgcgctggc agtgctgcgc gccaccttcc tccgcggtgt
     1141 agacgcggaa ggtcttgtcc ttcgagccgc aggcgtcgaa ctgcttctgc gcatccgcga
     1201 gcggaatctg ctcgtcatcc gcgccatgca ccagcaggaa cgggcagcgc attttctgca
     1261 tcacgccatc gagcctgaag ggctcgagct tcttcagcgc ggcatcgagc gagtccacgc
     1321 ccaggatcca catgatgtgg tgtccgggca ccgacagtga agccttgaat tgcgcttcga
     1381 tgcgcttctt ccaggtgtcg tggtagtccc attgcgcgcc ccacgcgatg cacgcggcgt
     1441 agcgcggctc catcgctgcg cagcgcggcg aatagtagcc gccgaggctg atggcgacga
     1501 tgccgatttt ccggggatcc acgtcggcgc gcttttcgag atagtccacg cacgcggatc
     1561 ccgcgacctc gtagtcatgg cgcagataat gcccgcggaa gcggatcgct tctccggtgc
     1621 cgggtccgtc catgacgagc accgagatgc cgcgcttcac gagatccggc acgccgcgca
     1681 cgaactggat ttccttggtg acgtcgagcc cgtcgaagaa caccacgcac ggcgcgcgtg
     1741 cggacttcgc gttctgcgcg tgcacgaagt aaccggggag gctcccgtcc gcgaacggca
     1801 cttcgacgat ctcgatcttg agatccgtga gcgccacgtg ccggtggaag caatcgacgc
     1861 ccctgcgata ggcttcgagc ccggtcttgt ccttgggcgt gcggaagcgc tcggccatct
     1921 gatagtagtt gcaggcgcga agatacgcgt gcgcggcggc atagcggtgt ccgctcttgt
     1981 cccacttctc ggcgtatccg cgcacgccgt ccgcaacctt gacgcacgcg tcgaaccagg
     2041 ccgcgtcgtc atccggtgcc tttcctgaga gcaatcgtcc gattttgtgc agctccccga
     2101 tctccgcgcc gccgtaggcg atggagccca tcatgttgat gaaggcggcg gaccagcggt
     2161 agttgccggg aaaatacatg aagtatgcgg gctcttttgc cattcggttt tcctttcggg
     2221 tttcttgcaa tgcgttcctc gcggtgctgc aattcgcgat cggccgagga tcagattttc
     2281 gggatgccgg ctttcttcgc aacgtccgcc cacttctcgg tctcttcctt gaggaaggcc
     2341 gcgtattcgg cgggtccgat gcccgagact tcgtaaccga tgtcctcgag ctgcttacgc
     2401 gtggccggat cgttgagcgg cttgctgagt accgcgtgga tgcggtcgac gatcgcctgc
     2461 ggcgtgccgg cgggggcgag gatgccgttc cagttgtagc tcgaccagcc tttcagcccc
     2521 tgttccgccg cggtgggcac ttccggcagc agcggcgagc gcttgatcgc ggtgacgccg
     2581 agcgcgcgca tcttgccgtc gcgcacgtaa ggccgcgcct ggaagatctg cacctgtgcg
     2641 tagtcgatgt tgccggcaat gaggtccacg acgtacagtg cggctcctct gtacggcacc
     2701 atggtcatct tgatccccgc gaccgccgca aagtgctcga tggcgaagtg cccgacggtc
     2761 cccacacccg gtgtcgcata gttgatcttg cccggactcc ttttggcgat tgcgatgaaa
     2821 tctttcacgc ctttggccgg tacactggga tgcgcgcaca tgcagcttgc gctgcgcgcg
     2881 atcagcgtga tcggcgcgaa gtcgcgcagg ccgtcatagt tgagcttctc ccgcacgctg
     2941 cgtgcgatgg tgagcggacc cggattcccg ccgagcagcg tgtagccgtc gggcgcggcc
     3001 ttcgcgaccg cctcggtgcc gatcacgccg cccgcgccgc cccggttttc cacaatcacc
     3061 tgctgcccga aggcggccgt gagctgctgc ccggcccagc gcgagtgcgt gtccgtcggg
     3121 ccgccggcgg cgaacggcac gatgatgcgt atgggcttgg tgggataact ctgcgccagg
     3181 gcgtgagagg cggcgagcgg catcagcagg ccggcgatga gggatgcaac gcgcatgacg
     3241 atcctccttg ggagtcggcg actcgagcga gacgccatta ttctgtccag cgcaagatag
     3301 tgtcggcgtt ccaccttgtc aatcgaaatg gcgccctttc ccgctctggt aaacttcggc
     3361 cttccacgcc aacccaaacc ctcaaggagt gtcatggctg ccaagaaagc gagggccgcg
     3421 gcaaggaagt accgcgacat cctctatgaa gtgaaagacc aggttgcatg ggtgacgatc
     3481 aaccggccac gcgtcctgaa cgcctttcgc gagcataccc tcgacgagat gatcgatgcg
     3541 ctcaagtcca cgcgcgaaga tccctccatc gtttgcgcgg tcgtgaccgg ggcgggcgac
     3601 aaggcgtttt ccgcgggtgg cgacttctat gcgatgaagc ggctcaattt cgccaacgcc
     3661 tacatgtgga acgaccgcat gctgggactc gcgatgacga tacgcgggct cccgatcccg
     3721 gtgatcgcga tggtgaacgg ctggtgcatg ggcggcgggc acgagctcgc gctgtggtgc
     3781 gatgtcgtga tcgc
//
LOCUS       DASURG010000154         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_233781, whole genome shotgun sequence.
ACCESSION   DASURG010000154 DASURG010000000
VERSION     DASURG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233781"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFY80_02555"
     CDS             complement(<1..737)
                     /locus_tag="VFY80_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006813.1"
                     /translation="MAILSSIALASIRPEAFDEQIQHLRELAEMLQPDASVRSSASYF
                     KALTALERMLLERNDVIGGLFSTTRPRGRAGILLEVSPIDLGREQRMLDHAVGMVDLP
                     AARVIWYLDVLRELRATNLWWRTKILELALTRQQRPPQNMAAAMQAFERLTVESMLEM
                     LPEQGDVPSVYRAPDARNHDIAMVRGFTAPRVLLNEEREQALMQALVPAPARTRARDA
                     GGGDESQPTAAAPERSAVRTHEQSEAAA"
     gene            complement(772..2082)
                     /locus_tag="VFY80_02560"
     CDS             complement(772..2082)
                     /locus_tag="VFY80_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006814.1"
                     /translation="MADKGLEERKAEAEARKAEADAKVAEAAALKAEREREEWESDLA
                     KRARAAEQERIRAEAEEKVRRAGQSLGDALALQKLEADAKKSIAEADKATADAQRAQV
                     SAFIPDFSKIEKGETTVTGDGTVLGSLLTPLAMEQAAKQVVGRLKLSERDRVLITTDA
                     DLASTDAVYVEVVTGLDLFTESAATLLGATPAEDRRRSSARDEQRRAAPAAVAGAIAA
                     AVPGLISLFSANRTLSSANLTADTAAAVAAVAAHLTGRTTVQIDDFVVVQGGDVAAKE
                     KALRQVRTQLVEHKLELEPPKKPVAEEQPLSVAAALIGQLITQIDAFLTSLHTSSGKR
                     SPFAIAALRQQLHPSSEAGGPVFNKVLYIKASGATADKLLHDRPLWFKDKIQVIGSAA
                     IVYWMMDTGTGNIIASGVGHGAVQIDGEIGDKLEFGKPYSLELT"
     gene            complement(2375..2650)
                     /locus_tag="VFY80_02565"
     CDS             complement(2375..2650)
                     /locus_tag="VFY80_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006815.1"
                     /translation="MRRIIGLLVLYGAAALGASFASSAYAADALFDAFADIGNGYGSH
                     WQGHEGETQSSPLTQLDPSLSRVPPTFAFFRKRLLDGIAIACILGLE"
BASE COUNT          493 a          866 c          767 g          552 t
ORIGIN      
        1 gcagccgctt ccgactgttc atgcgttcgc acggccgacc gctccggagc agccgcggtc
       61 ggttgtgact catcgccgcc acctgcgtca cgagcacgcg tcctcgccgg cgcaggaacg
      121 agcgcttgca taagagcctg ctctcgctct tcgttcaaga gcacacgcgg tgcagtgaag
      181 ccacgcacca ttgcgatgtc atggttccgt gcgtctggtg ccctgtagac acttgggacg
      241 tcgccctgct cgggcagcat ctcgagcatc gactcgacgg tgagccgctc gaacgcctgc
      301 attgccgccg ccatgttctg cggcggccgc tgctgtcgcg tcagcgcgag ctcgagtatc
      361 ttcgttcgcc accagagatt cgtggcgcgc agctcgcgca atacatcgag ataccagatc
      421 acccgcgccg cgggcagatc gaccatcccg acggcatggt cgagcatcct ctgctcgcgt
      481 cccaggtcga tcggagacac ttccagcaag atgcccgcgc gaccgcgcgg gcgcgtggtc
      541 gaaaataaac cgccgatcac atcgttgcgc tcgagcagca tccgttcgag agccgtcaaa
      601 gccttaaagt aacttgcgct gctgcgcacg ctcgcgtcgg gctgcagcat ctccgcgagt
      661 tcacgcaagt gctggatctg ctcgtcgaaa gcctccgggc ggatggaggc aagtgcgatg
      721 gaagagagga tcgccatgaa tcgtcacacg gccatgcgcg cgcgcttccg tctaggtcaa
      781 ttccaaggaa tacggctttc cgaactcgag cttgtctccg atctcgccat caatctgcac
      841 ggcgccgtgc ccgactccgc tcgcgatgat attgcccgtt cccgtatcca tcatccaata
      901 gacgattgcg gccgaaccga tcacttggat cttgtccttg aaccacaagg gcctatcgtg
      961 cagcagttta tcggccgtgg ctccggacgc cttgatgtac aaaaccttat tgaacaccgg
     1021 cccgccagct tcggagctcg ggtgaagttg ttgcctcaag gcggcaattg cgaagggcga
     1081 tcgcttgcca cttgaagtat gcagcgatgt aaggaatgca tcgatctgcg tgatgagttg
     1141 accgataagc gccgcggcaa ctgacagcgg ttgttcctcg gcaactggct tcttgggcgg
     1201 ctcgagttcg agcttgtgct cgaccagttg cgtccgtacc tgcctcagcg ccttctcttt
     1261 cgccgcgacg tccccgcctt gaaccacgac gaaatcgtcg atctgaaccg tggtccttcc
     1321 cgtcaggtgc gcggcaacgg ccgctacggc cgcggctgtg tcggccgtca ggttcgcgct
     1381 cgacaaagtc cggttggcgg aaaagagcga tatcaatccc ggcaccgctg cggcgatggc
     1441 ccccgcgacg gcagccgggg cggcacgcct ttgctcgtcg cgcgcagatg accgccggcg
     1501 gtcctccgca ggtgtagcgc ccagcaacgt tgcggccgat tctgtaaaga ggtcgagccc
     1561 ggtgacaact tcaacataga cagcgtccgt tgaggcgaga tccgcgtccg tcgtgattag
     1621 cacccgatcc cgctccgaga gcttgaggcg tccgacgact tgcttggctg cttgctccat
     1681 cgccaatggc gtcagcaacg atcctagaac ggtgccgtcg cccgtgaccg tcgtttcgcc
     1741 cttttcgatc ttgctgaaat ccgggatgaa cgcggagacc tgtgcccgct gcgcatctgc
     1801 ggtcgccttg tccgcctcgg caatggactt cttcgcgtcg gcttcgagct tctgcagcgc
     1861 gagagcgtcg ccgagcgact ggcctgcccg gcggaccttc tcttcggctt ctgcgcgtat
     1921 ccgctcttgc tccgccgcgc gtgcgcgctt cgccagatcg ctttcccact cctcccgttc
     1981 tcgctcggcc ttgagagccg ccgcttcggc gaccttggcg tcggcttccg cctttctagc
     2041 ttccgcttcg gcttttcgct cctctaatcc tttgtcagcc acgtttcact ccctaacgat
     2101 aaatcgaaca agtctcgccc agctcggcaa tgacgacaat gaagttggtt cgacgcttgg
     2161 agcagtcaat agcaacgaga cacagggcgc gacaagcgga tattccaaac ggtagaaggc
     2221 aatccggcgc ccgtttcgcc acgctgagcc tatgcgctgg agccagtgcg agagcacccg
     2281 caaagtgcgc ataacggttc atgctggctt catcctcccc cggtgaagac cgcgatcgcg
     2341 atcctgattg gatcagcgat cattgcgatt cactttactc cagccccagg atgcaggcaa
     2401 tcgctatccc atcaaggagg cgtttccgga aaaaagcgaa cgtcggaggg actcttgaaa
     2461 gagaaggatc cagctgcgtg agcggggagc tctgagtttc tccttcatgg ccttgccagt
     2521 gactgccata gccatttcca atatcagcga atgcgtcgaa aagcgcatcg gctgcgtatg
     2581 cactggatgc aaatgaagca cctagggctg ccgcgccata caacacaaga agtccaatga
     2641 tgcgtctcat gtcgaatcct cctcatcggg atttacgc
//
LOCUS       DASURG010000155         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_744049, whole genome shotgun sequence.
ACCESSION   DASURG010000155 DASURG010000000
VERSION     DASURG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744049"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..865
                     /locus_tag="VFY80_02570"
     CDS             107..865
                     /locus_tag="VFY80_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6006816.1"
                     /translation="MSRSAWKGDTRDVVLLHGWGTNAAVWRDLSRRLAPRFRMHAPLL
                     PFSAGTPEEGASLEEIADRLARWAPRRCMVCGWSFGGAVALAWALRAPEQIARFALIS
                     TSPCFVQRAGWRHGLEPRAVQDFANGLARESSAVLTRYIALQSRGDARAVRVARHLRQ
                     TLTLGGRPETPALARGLTLLRETDQRRAIASLRQPVLAVHGARDAIVPIAAAEFLAGA
                     LANARLAVIEAAAHVPFVSELEGVSDQLAGFFDE"
     gene            858..1733
                     /gene="bioC"
                     /locus_tag="VFY80_02575"
     CDS             858..1733
                     /gene="bioC"
                     /locus_tag="VFY80_02575"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEX6006817.1"
                     /translation="MSDELSIDKRSSRRAFERAAHTYDEAAALQNEVCGRMLSRLDYI
                     KHRPEAILDAGSGTGNALRALRERYSAARIVALDIALAMLKRGRARLPWWKGMVGRGT
                     QPVCGDIEALPFAEGAFGMVWSNLTLQWANDPQRAFTELRRVLAPGGLLMFSTFGPDT
                     LRELRQAYHGTDRHTHVNRFIDMHDIGDMLVAGGFSDPVMDMEHFTLTYADVRQLMHE
                     LKAIGAGNVTRGRPDALSGKSRLAAVMRNYERFRRDGKLPATFEVVYGHAWKPLPRVS
                     PSGRRVIEVKPRVMR"
     gene            1736..2434
                     /gene="bioD"
                     /locus_tag="VFY80_02580"
     CDS             1736..2434
                     /gene="bioD"
                     /locus_tag="VFY80_02580"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX6006818.1"
                     /translation="MTRAGARGFFVAGTDTGVGKTLVACALLRAFSQRGCRSVGMKPV
                     ASGAARTAGELQNEDVERLIAAGNVQAPRALVNVYCFEPAIAPHLAARHAGVEIALAP
                     IERAYRALADRADIVIVEGVGGLCVPLNDREDTADLATLLNLPIVLVVGMRLGCLNHA
                     LLTAAAMAARGLAFTGWVANHIDRDMACAEENVATLAQRLHAPCIARIPFERNPEPQR
                     IARAFHIDELVGNA"
     gene            2557..3027
                     /locus_tag="VFY80_02585"
     CDS             2557..3027
                     /locus_tag="VFY80_02585"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX6006819.1"
                     /translation="MECVDRGRADQFSFTARRNNSLTSSGRLFVFGLLCFVSVGIGLA
                     FTLVFGAWPIMPFAGLEMLVLYLAFRYVERHADDYERLAIAGDRVEVEVRDGGQLRRF
                     GFNRCWAQVVCDRAGSRLALKSHGREVEIGRHLNEEQRLSVARQLGRKLRDGRQ"
     gene            3086..>3381
                     /locus_tag="VFY80_02590"
     CDS             3086..>3381
                     /locus_tag="VFY80_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEX6006820.1"
                     /translation="MTSTLARLFAGVTALLAETARAAQYNLQPPQSIIAREIYDLHTL
                     IMWIILLIFVVVFGAMTYAIVKHRKAVGHKAEQFHENTTVEIVWTIIPFLILIG"
BASE COUNT          556 a         1062 c         1138 g          625 t
ORIGIN      
        1 ccaccgtccc gcaaggcacc gcaagactgc gcatctcgct ttcagccgcg cacgagccgc
       61 gcgatgtgga aaagttggtg caggcgctcg gcgagatcgg ccggcgatga gccggagcgc
      121 atggaaagga gacacgcgcg atgtcgtgct gctgcatggc tggggaacga atgccgcggt
      181 gtggcgcgat ctgtcgaggc ggctcgcccc acgctttcgg atgcatgcgc cgctcttgcc
      241 gttcagcgcc ggcacgccgg aggagggcgc gagcctggaa gagatcgcgg atcgcctggc
      301 acgttgggcg ccccgacggt gtatggtctg cggctggtcc ttcggcggtg cagtcgcact
      361 cgcttgggcg ctgcgcgcac ccgaacagat cgcgcgcttc gccctcatct ccaccagtcc
      421 ctgcttcgtg cagagggcgg gctggcggca cgggctcgag ccgcgggcag tgcaggactt
      481 cgcgaatggg cttgcgcgcg agtcatccgc ggtcctcacg cggtatatcg cgctgcaatc
      541 ccggggcgac gccagggcag tgcgggtggc gcgccatctg cgtcagacgc tcaccctggg
      601 cgggcgaccc gagactcccg cgctcgcgcg cggcctcacc ctcttgcgcg aaacggacca
      661 gcgccgcgca atcgcgagcc tccgtcagcc cgttcttgcg gtgcacggtg cgcgcgatgc
      721 gatcgtgccg attgcagctg cggaattcct cgcgggcgct ttggcgaacg ctcgtctcgc
      781 ggtgatcgag gcggcggccc atgtgccttt cgtttcggag ctcgagggcg tgagcgacca
      841 gctcgcaggg tttttcgatg agtgacgagc tttcgatcga caagcgctcc tcacggcgtg
      901 ctttcgaacg ggcagcgcac acctacgacg aggctgccgc gctgcagaat gaggtatgcg
      961 gccgcatgct ctcacgtctc gattacatca agcaccgtcc ggaggcgata ctcgatgcgg
     1021 gaagcggcac cggcaatgcg ctgcgcgcgc tgcgggagcg ctattccgcc gcgcgcatcg
     1081 tcgcgctcga catcgcgctt gccatgctga aacgcggtcg cgcgcgcctg ccgtggtgga
     1141 aaggcatggt ggggcgcgga acccagccgg tttgcggcga catcgaggct ttgcccttcg
     1201 ccgaaggcgc attcggcatg gtgtggtcca atctcacgct gcagtgggca aacgatccgc
     1261 agcgtgcgtt caccgagctg cgtcgcgttc tcgcacctgg ggggctcctc atgttcagca
     1321 cgttcggtcc ggacaccctg cgcgagctgc ggcaggctta tcacggcacc gatcgtcata
     1381 cgcatgtcaa ccgcttcatc gacatgcatg acatcggcga catgctggtc gccggaggat
     1441 tcagcgatcc ggtcatggac atggaacact tcacgctgac ttacgccgac gtgcgccagc
     1501 tgatgcatga gctgaaggcg attggcgcgg gcaacgtcac acgtgggcgc cccgatgcgc
     1561 tgagcggaaa gtcacggctt gccgctgtca tgcgcaacta cgagcgcttt cgccgcgacg
     1621 gaaaactccc tgcgaccttc gaggtggtgt acggacatgc ctggaagccg ctcccgcgcg
     1681 tcagtccgag tgggcggcgc gtgatcgagg tgaaaccgcg ggtgatgcgc tgaagatgac
     1741 acgtgctggc gcgcgcggct tcttcgtcgc aggaaccgac accggagtgg gcaagacgct
     1801 cgtcgcgtgc gcgctgctgc gtgcattttc gcaacggggc tgccgctcgg tcggcatgaa
     1861 gccggtcgcc tcgggcgccg cgcggacggc aggcgagctg caaaacgagg acgtggaacg
     1921 gctgattgct gcgggcaatg tgcaagcgcc tcgggcgctt gtcaatgtgt attgtttcga
     1981 gccggcgatt gctccgcatc tcgccgcgcg gcacgcaggc gtcgagatcg cgctcgcgcc
     2041 gatcgagcgc gcgtaccgcg cgcttgcgga tcgggccgac atcgtgatcg ttgaaggtgt
     2101 cggcgggctg tgcgtgccgc tcaacgaccg ggaggacacc gcggatctcg cgacgcttct
     2161 gaatctgccg atagtcctcg tggtcggcat gcgactcggc tgcctcaatc atgcgctgct
     2221 cacggcggcg gcgatggccg cgcgcgggct tgcattcacg ggctgggtgg caaaccatat
     2281 cgatcgcgac atggcgtgcg cggaggagaa cgtcgcgacc ctcgcgcagc gcctgcacgc
     2341 gccgtgcatc gcgcgcattc cgtttgaacg caatcccgag ccacagcgca ttgctcgcgc
     2401 ttttcacatc gatgagctcg tcggaaacgc gtaacgcgct cgcccgcacg cgagcatcga
     2461 tattttcgct cgctgaattg ttgctacgct gtgtcgggta tgctaaattc cgcgcatttc
     2521 gctcgggtgt caacgctcgt aaccaaggcg ctctggatgg aatgcgttga tcgcgggagg
     2581 gcggaccaat tcagtttcac ggcccgccgc aacaactccc tcacttcatc cggccgtttg
     2641 ttcgtttttg ggttgctttg tttcgtttcg gtcggaatcg gattggcatt taccctcgta
     2701 ttcggtgcct ggccgatcat gccgtttgcg gggctcgaga tgctggtgct gtacctcgct
     2761 tttcgttacg tcgaacgcca cgccgacgac tacgagcggc ttgccatagc gggcgaccgg
     2821 gtggaggtgg aggtgcggga cggcggacag ctgcgccgct tcggattcaa ccggtgctgg
     2881 gcgcaggtcg tgtgcgaccg ggccggcagc cggctggcgc tgaaatcgca cggacgcgaa
     2941 gtcgagatcg gaaggcattt gaacgaagaa caacggctct cggtggcccg ccaactcgga
     3001 cgcaaacttc gcgacggccg ccagtagcgc gtcacgaagc gagcgtgtct cagccgaaga
     3061 gcatccattc acaggtgagg gaagcatgac cagcacgttg gctcgactct ttgcgggcgt
     3121 aaccgcgtta ctcgcagaga cagcgcgcgc ggcgcagtac aacctgcagc cgccgcaaag
     3181 catcatcgcg agggagatct acgatctcca cacgctcatc atgtggatca tcctgctgat
     3241 cttcgtggtc gtgttcggcg cgatgaccta tgcgatcgtc aagcaccgca aggcggtcgg
     3301 gcacaaggcc gagcagtttc acgaaaacac cacggtcgag atcgtctgga ccatcattcc
     3361 gtttctgatc ctgatcggca t
//
LOCUS       DASURG010000156         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1254595, whole genome shotgun sequence.
ACCESSION   DASURG010000156 DASURG010000000
VERSION     DASURG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254595"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFY80_02595"
     CDS             <1..1082
                     /locus_tag="VFY80_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX6006821.1"
                     /translation="WHPADLGAAVLNALVDRTGIDPSLVDDVIVGCVSQIGDQSCHIG
                     RSMVLASKLPDSVPAVTIDRQCGSSQQSIHFAAQAVMSGTQDLVIAAGVESMSRVPMG
                     TPVFLPLQAGIGSGPWPKSIQERYGVSEFTQFRGAEMMAKKYGLSRDELDAFALESHR
                     KAAAATQAGAFDAEIVQIGDHRSDEGIRYDASLEALAGLKTLVPDGVITAGNASQICD
                     GAAAVLVASEAAVKAHGLKPLARIDQITVTAGDPVIMLDEPIRATRTALERSGRSIGE
                     IDLYEVNEAFAPVPLAWLAELGADAAKLNVHGGAIALGHPLGASGAKLMATLVHALHR
                     HGKRYGLQTMCEGGGIANVTIIERL"
     gene            complement(1131..1574)
                     /locus_tag="VFY80_02600"
     CDS             complement(1131..1574)
                     /locus_tag="VFY80_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006822.1"
                     /translation="MGQKVYLPIAIVVFLAATTGADLVARTFIGGQPLAVALQEHFYW
                     ARVQFVGTMLLLAPFVAVAFVCAFTERHARTRSALLIFAVAMLTLLYFYFQGHQAAER
                     AALQKMWTAATLSIGLLPFFIGAPVVLAAVGAGALAARFDRRISA"
     gene            1777..>2241
                     /locus_tag="VFY80_02605"
     CDS             1777..>2241
                     /locus_tag="VFY80_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240245.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX6006823.1"
                     /translation="MKIACIGGGPAGLYFAISMKLRDPGHEIEVFERNAPGVTFGWGV
                     VFSDQTVENLERNDSKSAQIIASEFAHWDDIDVHIKGECIRSSGHGFIGIGRKRLLEI
                     LQDRARELGVTLHFNSECGPADPKWQAYDLVIAADGANSRFRDAHADAFQVDI"
BASE COUNT          452 a          761 c          667 g          361 t
ORIGIN      
        1 actggcaccc ggccgatctc ggcgcggcgg tcctgaatgc gctggtcgat cgcacaggca
       61 tcgacccgtc gcttgtcgac gacgtgatcg tgggctgcgt cagccagatc ggcgaccaga
      121 gctgccacat cggccgaagc atggtgctcg cgtcgaagct tcccgacagc gtccccgccg
      181 tcaccatcga ccggcagtgc ggaagctcgc agcaatcgat ccacttcgcc gcccaggcgg
      241 tgatgagcgg cacccaggac ctcgtcatcg cggccggcgt cgaaagcatg agccgcgtgc
      301 ccatgggcac gcccgtcttc ctgccccttc aggcggggat cggctccggt ccctggccca
      361 agtcgatcca ggagcgctac ggtgtttccg agttcacgca gttccgcggc gccgagatga
      421 tggcgaagaa atacggcctc tcccgcgacg agctcgacgc ctttgcgctc gaaagccacc
      481 gcaaggcggc tgccgcgacc caggccggag ccttcgatgc ggagatcgta cagatcggcg
      541 accaccgctc cgacgaaggc atccgctacg acgcgtcgct ggaagcgctc gcgggactca
      601 agacgctcgt ccccgatggc gtaatcaccg ccggaaacgc cagccagatt tgcgacggcg
      661 cggcagcggt gctcgtcgca agcgaagccg cggtgaaggc gcacggcctc aaaccgctcg
      721 cccgaatcga ccagatcacc gtcaccgcag gcgaccccgt catcatgctc gacgagccca
      781 tccgcgccac ccgaaccgcg ctcgaaaggt ccggccgcag catcggcgag atcgacctct
      841 atgaagtcaa cgaagccttc gctccggtgc cgctggcgtg gctggcggaa ctcggcgccg
      901 acgcggcgaa actcaacgtg cacggcggtg ccatcgccct cggccatccg ctcggtgcat
      961 caggcgcgaa actgatggcg acactggtcc acgccctcca ccgccacggc aagcgctatg
     1021 gcctccaaac catgtgcgaa ggcggcggca tcgcgaacgt cactattatc gaacggctct
     1081 agggagtctc gactttcgac ccagtgctgc cattagcgcg tgtctcgacc ctaagcggaa
     1141 attcggcggt cgaaccgggc ggcgagcgcg cccgcaccga cagcagcaag aacaacggga
     1201 gcgcctatga agaagggaag cagtccgatg gatagcgttg cggccgtcca cattttctga
     1261 agcgcggcac gttcagctgc ctggtgccct tggaaataga agtagagcag cgtcaacatc
     1321 gcaaccgcaa agataagcaa ggcgctccgc gttctagcgt gcctctccgt gaaagcgcag
     1381 acgaacgcca cggcgacaaa cggcgcgagc aaaagcattg tgccgacgaa ctgcacgcgc
     1441 gcccaataga aatgctcctg caaagcgacg gccaacggct gacccccgat gaatgttctc
     1501 gcgacgaggt ccgctccggt ggtcgcagca agaaacacaa caatggcaat aggcagatag
     1561 accttttgtc ccatgcaatg acattactca gcctacggca ggtttccacc caaagcagac
     1621 attccatctg tcctacacgc cggctgttgt gccatgctgc tggagatgaa tttccgtcct
     1681 cctctagcca ctggtcccct cgtcccaccg gcaaccgtta aagacacaca aaatggcgca
     1741 tgaccgtgtg actttagtgg ctttaaggcg cgtgcgatga aaatcgcctg catcggtggc
     1801 gggccggctg ggctctactt cgcgatctcg atgaagctgc gcgatccggg tcacgagatc
     1861 gaggtgttcg agcgcaacgc gccgggcgtc accttcggct ggggcgtcgt cttctccgac
     1921 cagacggtgg agaacctcga gcggaacgat tcgaaatccg cgcagatcat cgcgtccgag
     1981 ttcgcacatt gggacgacat cgacgtccac atcaagggcg agtgcatccg gtcgtccggc
     2041 cacggcttca tcgggatcgg ccgcaagcgc ctgctcgaaa ttcttcaaga ccgcgcccgc
     2101 gagctcggcg tcacgctcca ctttaattcc gagtgcggcc ccgccgatcc caaatggcag
     2161 gcctacgatc tcgtcatcgc cgccgacggc gccaactcgc gattccgcga cgcgcatgcc
     2221 gacgccttcc aggtcgacat c
//
LOCUS       DASURG010000157         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_131403, whole genome shotgun sequence.
ACCESSION   DASURG010000157 DASURG010000000
VERSION     DASURG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131403"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFY80_02610"
     CDS             complement(<1..1059)
                     /locus_tag="VFY80_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6006824.1"
                     /translation="MNTATIIARALKDAGVRYAFGIPGGEVLELLEALRRTGIEFVLT
                     KQELGAGFMADASYQLTGRPGVLVATLGPGITNTATAVAQALLDRSALVVVTGEIATH
                     LKAVYTHQIIDQESLLRPIVKWSTQIARGGAYEQVRKGLAIACAPMPGPVHFNLPTDV
                     APAEQDEGREFAPVAVASVPAALDATAVPAWLKSARRPLAFVGVGVQLDGAEQAFKAF
                     IEKWRIPFIATYKAKGVVAEDHPLCVGATGLSPVIDRIHMARIKDADLVLTIGFDPVE
                     LRSDWMAPWDERKRTVNIDLVPNTHHVFRSAIEYSGSIRGALDALTRLARSKRPARWS
                     ASELDQYRLEQQKAISQRP"
     gene            1312..>2114
                     /locus_tag="VFY80_02615"
     CDS             1312..>2114
                     /locus_tag="VFY80_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX6006825.1"
                     /translation="MSHNLFSSLQEFSFDGRKGKYYSLPALEKAGLGKISRLPVSLRV
                     VLESALRNFDGKKITEDHLKTLAGWKPKAARSEEVPLVVARVLLQDMTGVPLVVDFAA
                     MREAAKAQGKDPEIIEPIVPGHLIMDHSVQIDHYGSPDALKKNMEIEFKRNRERYQFL
                     KWSAQAFKTFHIIPPGNGICHQINLEYVSRVVWHKDDMYYPDSLVGIDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPMYFLEPDVVGVHLTGRLRDGVTATDLVLRIT"
BASE COUNT          389 a          686 c          655 g          384 t
ORIGIN      
        1 tggccgttgt gaaattgcct tctgctgctc gagccgatac tgatcgagtt cgctggcgct
       61 ccagcgagcc ggccgtttcg acctcgcgag gcgcgtaagg gcgtcgagcg cgccgcggat
      121 gctgccggaa tattcgatgg cgctgcggaa tacgtgatgc gtattcggca cgagatcgat
      181 gtttaccgtg cgcttgcgct cgtcccacgg cgccatccag tcgctgcgca gttccaccgg
      241 gtcgaagccg atcgtgagaa cgagatcggc atctttgatg cgcgccatat ggatcctgtc
      301 gatcaccggg ctcaggcccg ttgcaccgac gcagagcgga tgatcttccg caaccacgcc
      361 cttcgccttg taggttgcga tgaagggaat gcgccacttc tcgataaacg ccttgaacgc
      421 ctgctccgcg ccatcgagct gtacgcccac gccgacgaat gccagcggac gccgcgcgct
      481 tttcagccag gcgggcacgg ccgtcgcatc gagcgctgca ggcacgctcg cgacggcgac
      541 cggcgcgaac tcgcggcctt cgtcttgctc ggccggcgca acgtcggtgg gcaggttgaa
      601 gtgcaccggg ccgggcatgg gtgcgcacgc gatggcaagc cccttgcgga cctgctcgta
      661 tgcgccgccg cgcgcgatct gcgtggacca tttgacgatc ggccgcagca gcgattcctg
      721 gtcgatgatc tggtgcgtat agacagcctt caaatgcgtg gcgatctcgc cggtgacgac
      781 gacgagcgcc gagcggtcga gcaatgcctg cgcaaccgcg gtggcggtgt tggtgatgcc
      841 cggccccagt gtggccacca gtacgcccgg cctgccggtc aactgataac tcgcgtccgc
      901 catgaagccc gcgcccaatt cctgcttggt cagcacgaac tcgatgccgg tgcggcgcag
      961 cgcttccaga agttcgagca cttcgccgcc cggtatgccg aaagcgtaac gcacacccgc
     1021 atccttcagg gcgcgcgcaa tgatcgtcgc tgtgttcacc aagacaattt ctccaacaga
     1081 atgtggaccg ctataggccg cgcgtaaatg tagcacgagc cttgacactg cacgacctca
     1141 tcagttactt tcgcgggcta tcctccggct tcgctctcgg tccgatcaca gcgcaatgaa
     1201 ccgcaccccg actccgatcg acgcgagccg cgccatcgcc attttttccc gacacggtca
     1261 tcctcgcgcg gcttgcgcgc gggcagtccg cgtcgtcatc caggagcaag catgagtcac
     1321 aatctgttca gttccctgca ggaattttcc tttgacggca ggaaaggcaa gtattactca
     1381 ctgccggctc tcgaaaaggc ggggctcggg aagatatccc ggcttcccgt cagcctgcgc
     1441 gtagtgctcg agtccgcact gcggaatttc gacggcaaga agatcaccga ggaccatctc
     1501 aagacgctcg ccggctggaa acccaaggcc gcgcgcagcg aggaagtgcc gctggtggtc
     1561 gcgcgcgtgc tgctgcagga catgacgggc gtgccgctgg tggtcgattt cgcggcgatg
     1621 cgcgaagcgg cgaaggcgca gggcaaggat ccggaaatca tcgagccgat cgtgccgggc
     1681 catctcatca tggatcactc cgtgcagatc gatcattacg gcagcccgga cgcgctcaag
     1741 aagaacatgg agatcgagtt caagcgcaac cgcgagcgct atcagttcct caagtggagc
     1801 gcacaggcat tcaagacctt ccacatcatt ccgcccggca acggcatctg ccaccagatc
     1861 aacctcgaat acgtctcgcg cgtcgtctgg cacaaggacg acatgtacta tccggacagc
     1921 ctggtgggca tcgactcgca caccaccatg gtgaacggcc tcggcgtgct gggctggggt
     1981 gtcggcggca tcgaagccga agcggcgatg ctgggccagc cgatgtactt cctcgagccg
     2041 gatgtcgtcg gcgtgcactt gaccgggcgc ctcagggacg gcgttaccgc caccgacctc
     2101 gtgctgcgca tcac
//
LOCUS       DASURG010000158         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1255214, whole genome shotgun sequence.
ACCESSION   DASURG010000158 DASURG010000000
VERSION     DASURG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255214"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFY80_02620"
     CDS             <1..320
                     /locus_tag="VFY80_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006826.1"
                     /translation="RALAVTSAKRSPAAPDIPTVAESGVPGFEEGGHHGIVAPSGVPK
                     EIIAKLNKAIVAAMRSPDIEKRLGVEGAQVVASSPEEYDALIRRDLAKWAKVIAAAGI
                     TPQ"
     gene            417..1274
                     /locus_tag="VFY80_02625"
     CDS             417..1274
                     /locus_tag="VFY80_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6006827.1"
                     /translation="MTFRELLATEKAYLAPAVFNPLTAKLAERAGCEVLYLGGGALGY
                     VKCCLEANLNLTEVVQAGVEIRAVTRLPLILDAACGFGDPMHMHHTVSLAEAAGFCAI
                     EIEDQVIPKRAHHHIGIERMIPLELMTAKVKEAVSARRDRDFVIIARTNAVRQTGMDD
                     AVARGRAYRDAGADMLYISPRNAEEAREIARRLPPPFMFSYESSGERPGAGLTLSELG
                     KLGYRVLSVANTALPFHRAMRETYEAIARGEPNPIMAGTTAKAEQSALHATLDFDALI
                     AIEKATVEK"
     gene            1323..2297
                     /locus_tag="VFY80_02630"
     CDS             1323..2297
                     /locus_tag="VFY80_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006828.1"
                     /translation="MSLAAISRGFLIATALLGAGVAQAQQNYPVRPIRLVVPSSPGGG
                     TDITARIIAPKLGEYLGQQVVVENRPGAGTMIGGEVVARAVPDGYTLLMGISTLAINP
                     AMYRKVPYDALKDFAPISQVVALPNVLVTHPSLPVKTVRELIAFAKSRPGQINFASAG
                     VGTNPHLSMELFLSMTGVKMIHVPYKGSGQGVIDLIAGHVTVMTPSILTALGYVKDRR
                     LRALGVTSAKRAGGAPDIPTIAEAGVPGYEAVQWFGVLAPAGTPRDIVSRLHKEIVRT
                     VQTPDIRQKLVNDGADPVGSLPEEFAAFIRSETAKWAKVAKAIGLKPE"
     gene            2370..3233
                     /locus_tag="VFY80_02635"
     CDS             2370..3233
                     /locus_tag="VFY80_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6006829.1"
                     /translation="MALALAILSSLCFGIALVTGRVGLRTLDARSGAAISIPTATLLF
                     ITAAPFAFDLLQFDVRAVLLFAIVGLFFPAVVTLLTFRSNEILGPTTTGVISSTAPLF
                     ALIGAALLLGERVPGEAALSALGVVAGIALISWRREEGARSRFVGWALFWPLAGALVR
                     GLAQVGAKAGLLIWASPYAASLIGYVVSSAAVVSADRLRPERPRPTKRALLWFAATGV
                     LNGAAVLLMYAALSGAPVWMVAPVVAAYPLVTAIMSAAVLREETLSIQTVSGAALTVA
                     AIAYLVASAAV"
BASE COUNT          563 a         1049 c         1091 g          555 t
ORIGIN      
        1 tccgtgcgct cgccgtcacc tcggccaagc gctctcccgc agcaccggat atcccgaccg
       61 tcgcggaatc cggcgtgccg ggattcgagg agggcggcca tcacggcatc gtcgcgccgt
      121 cgggcgtgcc gaaggagatc atcgcgaagc tcaacaaggc catcgtcgct gccatgcgct
      181 cgcccgacat cgaaaagcgc ctgggcgtcg aaggcgcgca ggtggttgcg agctctcccg
      241 aagagtacga cgccctcatt cgccgcgacc tcgccaaatg ggccaaggtg atcgccgcag
      301 ccggcatcac cccgcagtga ggtcgtcatt ccgttgaaaa acggaatcaa gtctcaccgc
      361 gaaatcgcaa ccgagtagca gaggcgagca tgaggagtgg acaggagggc aaactgatga
      421 cattccgcga gctgctcgcc acggagaaag cgtatctcgc tccggcggtg tttaatccgc
      481 tcacggcgaa actcgccgag cgcgcgggct gtgaggtgct ctatctcggc ggcggcgcgc
      541 tgggctacgt caagtgctgc ctcgaggcaa atctgaatct tacggaggtc gtgcaggccg
      601 gcgtcgagat ccgggccgtg acgcggctcc cgctcattct cgacgcggcg tgcggattcg
      661 gcgacccgat gcacatgcat cacaccgtca gccttgccga ggcggctggg ttctgcgcga
      721 tcgaaatcga ggaccaggtg attcccaagc gtgcgcatca ccacatcggc atcgagcgca
      781 tgattccgct cgagctgatg accgcgaaag tgaaggaggc ggtaagtgcg cgacgggatc
      841 gcgatttcgt catcatcgcg cgcaccaacg ccgtccgcca gacgggaatg gacgatgccg
      901 tagcacgcgg gagagcctac cgggatgcag gcgcggacat gctctacatt tcgccgcgca
      961 atgcagagga agcgcgcgag atcgcgcgca ggctcccgcc gccgttcatg ttctcgtacg
     1021 agtcgagcgg cgagcgcccc ggcgcgggcc tcacgctgag cgagctcggc aagctcggat
     1081 accgcgtgct ctccgtcgcg aataccgcac tgcccttcca ccgcgcgatg agagagacgt
     1141 atgaggcgat tgcccgcggc gagccgaatc cgatcatggc cggcacgacc gccaaggcgg
     1201 aacagtcggc gctccacgcg acgctcgact tcgatgctct catcgccatc gaaaaggcca
     1261 ccgtggaaaa atagggtggt cgtccacgca cgtaacggaa agccgatttc ctaggaggac
     1321 gaatgtcact tgccgcaatc tcgcgcgggt tcctgatcgc gacggccctg cttggagcgg
     1381 gcgtggcgca ggcgcagcaa aattatccgg tgcggccgat ccggctcgtc gtgccctcat
     1441 cccccggcgg gggcacggat atcaccgctc gcatcatcgc gccgaagctc ggcgagtacc
     1501 tcggccagca ggtggtggtc gaaaaccgcc cgggggccgg gacgatgatc ggcggcgaag
     1561 tggtcgcgcg tgcggtgccg gacggctata cgctcctcat gggcatcagc acgctcgcca
     1621 tcaacccggc gatgtacagg aaagtcccgt acgacgcgct gaaagatttt gcgccgatca
     1681 gtcaggtggt cgcgctgccc aacgtgctcg tgacgcatcc ttccctgccg gtgaagacgg
     1741 tgagagaact gatcgcgttc gcgaagtcac gccccggcca gatcaatttc gcatcggcgg
     1801 gcgtgggaac caatccgcac ctttcgatgg agctcttcct ctccatgacg ggcgtgaaaa
     1861 tgatccacgt gccgtataag gggtcggggc agggcgtgat cgatctcatc gccggacacg
     1921 tcaccgtgat gacgccgagc atccttactg cgctcggtta cgtgaaggac aggcggctgc
     1981 gggcgcttgg cgtgacgagc gcgaagcgcg ccggcggcgc gcccgacatc ccgacgatcg
     2041 ccgaagcggg cgtccccgga tacgaggcgg tgcagtggtt tggcgtgctc gcgcctgcgg
     2101 gaacaccgcg cgacatcgtc agccgtctgc acaaggagat tgtgcgcacc gtgcagacgc
     2161 cggacatccg ccaaaagctc gtgaacgatg gcgccgaccc ggtggggagc ctgccggagg
     2221 aatttgccgc attcatccgc tcggaaaccg ccaagtgggc gaaggtggcg aaggcgatcg
     2281 gactcaagcc ggagtagcag gcaaacgcga tcgctccata tcccgcagcg ggtatagtgg
     2341 ccccggggac cagctctctt ggaggcggca tggcgctcgc gcttgccatc ctgagttcac
     2401 tgtgctttgg catcgcgctc gtgacgggcc gggtgggatt gcgcacgctc gacgcgcgct
     2461 ccggcgcggc catcagcata cccaccgcaa cccttctctt cattaccgcg gcgccgttcg
     2521 cattcgacct cctgcaattc gatgtgaggg ccgtgctgct gttcgcgatc gtcggcctct
     2581 tctttcccgc cgtcgtcacg ctgctcactt ttcgctccaa cgagatcctc gggcccacga
     2641 cgacgggggt gatatcgagc acggcgccgc ttttcgcgtt gatcggcgca gcgctgcttc
     2701 tcggcgaacg cgtgccgggt gaggcggcgt tgtccgcgct cggcgtggtg gcgggcatag
     2761 ccctcatttc gtggcggcgc gaagaggggg cgcgatcgcg cttcgtggga tgggcgctgt
     2821 tctggcccct cgcgggggcg ttggtgagag gactggcaca ggttggcgcc aaggctgggc
     2881 tcctcatctg ggcgagtcca tacgcggcga gccttatcgg ctatgtcgtg tcgtccgccg
     2941 cggtagtgag cgcagaccgc ctgcgcccgg aaagacccag gcccacgaag cgtgccctgc
     3001 tctggtttgc cgcaaccggt gtactgaacg gcgccgctgt cctgctcatg tatgccgcgc
     3061 tgagcggggc gcccgtctgg atggtcgcgc cggtcgtggc cgcctatccg ctggtgaccg
     3121 ccatcatgag cgccgcggtg cttcgcgagg aaacgctgtc gatacagacc gtttcggggg
     3181 ccgcgctgac cgtggcggca attgcatatc tggtcgcttc ggcagccgtc tgaaagccgg
     3241 tcagagcacg ccctgcac
//
LOCUS       DASURG010000159         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_30237, whole genome shotgun sequence.
ACCESSION   DASURG010000159 DASURG010000000
VERSION     DASURG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30237"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFY80_02640"
     CDS             complement(<1..264)
                     /locus_tag="VFY80_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_147845912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX6006830.1"
                     /translation="MNTIPVYDPRGVVEATALSVAPRASTLKGLRLGLLDNTKWNANK
                     LLRGLRDRLAAKHGFAEVNYYRKESFSLNAKSALIDEIAAHNDI"
     gene            complement(282..1406)
                     /locus_tag="VFY80_02645"
     CDS             complement(282..1406)
                     /locus_tag="VFY80_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006831.1"
                     /translation="MNDPAAVASPAAETSHVPAGTDLIEWYYERGYSDGFPLVPPTPQ
                     KIEAVLEALGGEAGERMCRVPPRWGSLTREVLATNMVMAGCRPEYAPVVEAAMLALCA
                     PPFNLNGVQATTHMASPLLVVNGPIRGAIGMNAGCNVFGSGNRANATIGRALRLILLN
                     VGGGWPGLLDKSTLGHPGKYTYCIAENEEASPFAPYHVEKGYRAEHSTVFVLAAEPPH
                     SITNHFANDAEGILDSVCSAMSTICNNNAVSAGHCAVVLGPEHAATIAKCGWKRHDIR
                     HYLWAHSGNRWDDLYGKGRYGKVYNRNLPVWYKREPDARTPIVPSPDNIHLFVAGGEA
                     GRFSAFIPGWGHMSTPVLRAIDGAAPGAGGPQCIDGTCYL"
     gene            1527..2756
                     /locus_tag="VFY80_02650"
     CDS             1527..2756
                     /locus_tag="VFY80_02650"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6006832.1"
                     /translation="MTRTENDVLDTLNRSAGFADVGYEEALRRARELIPFLREQASKC
                     EAARCLTPAVINALHTTGLLRYLQPKVWGGMELPFVSYFDIPEMLARGDISVGWVVAN
                     LASHHRNLAWWDPKAQEEVWGRNPDAGIASGIAFQQGRGRQADGGIVLSGEWNFSSGS
                     DHSDWCMLACVVRDGDKPVDYVLCLLHWSEYEVVDDWHTLGMRATSSKTVRCKDIFVP
                     DYRIVSMHLARPDHKWPGLAVHRNPHYRIPTSALGGDCIGACMVGNARAALELAVEHV
                     KSRSTNYTGAKMRDFQTVQLRIGAAGARIDAAALLMRNDCLEAQYIVEADGKLDTETK
                     LRYKRNCAFAVRECLEAVDMLHEMVGAMGIYDQHPLQRIFRDARAAAGHISFSTDAQL
                     PPWGLVALGGDFASPTL"
     gene            complement(2789..3418)
                     /locus_tag="VFY80_02655"
     CDS             complement(2789..3418)
                     /locus_tag="VFY80_02655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6006833.1"
                     /translation="MRLRPQDLDQITRLTLENYEQNAEMFWAGTHDHDVSQNIAALLE
                     HIERDPPYTLLDFGCGPGRDLKTFSNLGHTAIGLEGTRRFAEMARAFSGCEVWEQDFL
                     RLELPAARFDGIFANAALFHVPVQELPRVLRELQAALKPRGVLFSSNPRGSNEEGWNA
                     GRYGAYHDLDAWRRYMTEAGFTELLHYYRPAGLPRERQPWLATVWRKSQ"
     gene            3452..>3930
                     /locus_tag="VFY80_02660"
     CDS             3452..>3930
                     /locus_tag="VFY80_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6006834.1"
                     /translation="MSEARSPMHAGEAVAEALGAEGVTRVYSVPGSHIHPIYDGLSRV
                     ETVRFVTCKQEPNTSLMADAYGRLTGKPAVSLVTAGPGSLNSMAGVAQAYAAASPMVH
                     IAGAVPLKADLEAFHGVDDPAFVHEMFKKITKWSARVERLEDVPGVMAKAFHIARSGR
                     "
BASE COUNT          687 a         1264 c         1272 g          707 t
ORIGIN      
        1 aatatcgttg tgcgccgcga tctcgtcgat cagcgcggat ttcgcattga gggagaagct
       61 ttccttgcgg taatagttga cctcagcgaa gccatgcttc gctgcgagtc gatcgcggag
      121 gccgcgcaaa agcttgttcg cgttccactt ggtgttgtcg agcaagccga gccgcaggcc
      181 cttgagtgtc gaggcgcgcg gcgcaacgga gagcgctgtt gcctccacga cgccccgcgg
      241 gtcgtacacc ggtatcgtgt tcatcatcgt ctccactcga gttacaaata acacgtgccg
      301 tcgatgcact gcgggccgcc tgccccgggc gccgcaccgt cgattgcgcg cagcacgggc
      361 gtggacatgt ggccccatcc cggaatgaac gcggagaagc gtcccgcctc gccccccgcg
      421 acgaacaggt ggatgttgtc cgggctcggg acgatcggtg tacgggcgtc cggctcgcgc
      481 ttgtaccaca ccggcaggtt ccgattgtag accttgccat agcggccctt cccatacaga
      541 tcgtcccagc ggttgccgga atgcgcccag agataatgac ggatgtcgtg gcgcttccag
      601 ccgcatttcg cgatcgtcgc ggcgtgctcc ggcccgagca ccaccgcgca gtgtccggcg
      661 gagaccgcgt tgttgttgca aatggtgctc atggccgagc acacgctgtc gaggatgcct
      721 tcggcgtcat tcgcgaagtg gttggtgatg ctgtggggcg gctctgcggc gaggacgaag
      781 accgtcgagt gttccgcgcg gtagcctttc tcgacgtggt acggcgcgaa cgggctcgcc
      841 tcctcgttct ccgcgatgca gtaagtgtat tttccggggt ggcccaacgt gctcttgtcg
      901 agcaggcccg gccaaccgcc gccgacattg aggaggatca ggcgcagggc gcgaccgatc
      961 gtggcgttgg cgcgattccc cgaaccgaac acgttgcagc cggcattcat gccgatcgcg
     1021 ccgcggatcg ggccgttcac cacgagcagc ggactcgcca tgtgcgtcgt tgcctggaca
     1081 ccgttcagat tgaacggcgg cgcgcagagc gcaagcatcg ccgcttcgac caccggggcg
     1141 tattcggggc gacatccagc catgaccatg ttggtggcga gcacttcccg cgtgagactg
     1201 ccccagcggg gcgggacacg gcacatacgt tcacccgcct cgcccccgag cgcttcgagt
     1261 accgcctcga tcttctgcgg cgtcggcggc accagaggaa aaccgtccga gtagccacgc
     1321 tcgtagtacc actcgatgag atcggtcccg gccggcacgt ggctcgtttc ggctgctgga
     1381 gaagcgacgg cggcgggatc gttcatggcg gattccggaa aattgcacga gagcgagtca
     1441 caaatattct acttcgtttc atgcggctct acgtttttca cgcgtgaagc cgcataattc
     1501 aggctccgca gaacgacggg cgcgcgatga cacgaacgga gaacgacgtc ttggataccc
     1561 tcaaccggtc cgcgggattt gcggacgtgg gctatgaaga agcgctgcgc cgcgcgcgcg
     1621 agttgattcc gttcctgcgc gagcaggcgt cgaagtgcga agcggcgcgc tgcctcacgc
     1681 ccgcggtgat caatgccttg catacgaccg gacttctccg ttatctgcag cccaaggtgt
     1741 ggggcggaat ggaattgcct ttcgtctcct atttcgacat tcccgaaatg ctcgcccgcg
     1801 gcgacatctc ggtcggctgg gtcgtggcaa acctcgcttc ccaccatcgt aatctcgcat
     1861 ggtgggatcc gaaggctcag gaagaggtat ggggccgcaa ccccgatgcc ggcatcgcgt
     1921 ccgggatcgc gttccaacag gggcgcgggc ggcaagcgga cggcggcatc gtgctttccg
     1981 gagagtggaa tttttcctcg ggcagcgacc actcggactg gtgcatgctc gcctgcgtgg
     2041 tgcgcgacgg cgacaagccg gtcgattacg tgctgtgcct cctgcactgg tcggaatacg
     2101 aagtggtgga cgactggcac acgctcggca tgcgcgcaac gagcagcaag accgtgcgct
     2161 gcaaggacat cttcgtgccg gattaccgca tcgtctcgat gcatcttgcc cgtccggacc
     2221 ataagtggcc cggactcgcg gtgcacagga acccgcatta ccggatcccg acttctgcgc
     2281 tgggcgggga ctgcatcgga gcgtgcatgg tgggcaatgc acgcgcagcg ctcgagctcg
     2341 cggtcgagca cgtgaagtcg cgcagcacca attacaccgg cgccaagatg cgggatttcc
     2401 agacggtgca actgcgcatt ggagccgcag gggcccggat cgatgccgcg gcgctcctca
     2461 tgcgcaacga ctgcctcgaa gcgcagtaca tcgtggaagc ggacggcaag ctcgataccg
     2521 aaacgaagct ccgctacaag cgcaactgcg cctttgcggt gcgcgagtgt ctcgaagcgg
     2581 tggacatgct gcacgagatg gtgggcgcga tgggcatcta cgatcagcat ccgctgcagc
     2641 gcatcttccg cgatgcgcgc gccgccgcgg ggcacatcag cttcagcacc gacgcccagc
     2701 ttccgccgtg ggggctcgtc gcgctcggcg gcgatttcgc gagcccgacg ctctagccgc
     2761 cgtgtggaac tcacctggcc gctacacctt actgactctt tcgccacacg gtcgcgagcc
     2821 acggctgccg ctcgcgcggc aaccccgccg gccggtagta gtgcagaagc tcggtaaagc
     2881 ccgcctcggt catgtagcgc cgccacgcgt cgagatcgtg atacgccccg tagcgcccgg
     2941 cgttccaacc ctcctcgttg ctgccgcggg gattggaact gaatagcacg ccgcgcggct
     3001 tcaacgcggc ttgaagctcg cgcagcactc gcggcagttc ctgcaccggc acgtggaaga
     3061 gcgcggcgtt tgcgaagatg ccgtcgaacc gggccgccgg aagttcgagc cgcaggaaat
     3121 cctgctccca gacttcacag ccgctgaaag cacgtgccat ttcggcgaag cgccgcgtgc
     3181 cttccagccc gatcgccgtg tgcccgagat tcgagaaggt cttgaggtcg cgccccggac
     3241 cgcagccgaa atcgagcaac gtgtacggtg gatcgcgctc gatgtgctcg agcagcgccg
     3301 caatgttctg gctcacatcg tgatcgtgcg tgccggccca gaacatctcc gcgttttgct
     3361 cgtagttctc cagcgtgagg cgggtgattt gatcgagatc ctgtgggcgc agtcgcatgc
     3421 cgtattatgt cgcgtcacag gagacaaacc aatgagtgaa gcacgttcgc caatgcacgc
     3481 cggagaggcg gtggccgaag cgctgggcgc ggaaggagtg acgcgcgtct atagtgtccc
     3541 cggctcccac attcacccga tctacgatgg gctgagccgc gtcgaaaccg tccgcttcgt
     3601 cacctgcaag caggaaccca acacgtcgct catggccgac gcctatgggc gcttgaccgg
     3661 caagcccgcg gtttccctcg tcaccgccgg gccaggcagt ctcaactcga tggccggcgt
     3721 cgcacaggcc tatgccgccg cctcccccat ggtgcacatc gccggcgcgg tgccgctcaa
     3781 ggcggacctc gaagcgtttc atggcgtcga tgatccggca ttcgtccacg agatgttcaa
     3841 gaagatcacc aaatggagcg cgcgcgtcga gcgtctcgag gacgtcccgg gcgtgatggc
     3901 caaggcgttc cacatcgcgc ggagcggccg
//
LOCUS       DASURG010000160         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_337038, whole genome shotgun sequence.
ACCESSION   DASURG010000160 DASURG010000000
VERSION     DASURG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337038"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..238)
                     /locus_tag="VFY80_02665"
     CDS             complement(68..238)
                     /locus_tag="VFY80_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006835.1"
                     /translation="MPDRKHLAQSLRDDAAKVRNAIQRGGEEIARRMDEAADLLDGRA
                     EKGIEPPRAPDA"
     gene            complement(262..1263)
                     /locus_tag="VFY80_02670"
     CDS             complement(262..1263)
                     /locus_tag="VFY80_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6006836.1"
                     /translation="MFDPKKMRRPGAGLPALVGMAAGLAATALLVRAQTRRAEAQHPP
                     AGRFIEVCGVRLHYIEKGLGVPVLLIHGTGVTAEDYVASGIVDRLAERYRVIAFDRPG
                     YGYSERPGKGWSARAQAELLQEACGALGIDRAIVVGHSWGTLVALEMALGDPGRVYGL
                     VLVSGYFFPSPRIDSLLIGAPAMPVLGTVMRHTMSPFVARLLAPRLIRRMFAPQPVPD
                     VFLDAVPVSIMLRPEQLRASAEDAARMVPTAGVVSRRYGELRDIPVSIMAGNEDGIVA
                     ADGQSSRLARALPHCEFEALDGAGHMLHYSHAERVARAVDRVSKEALPQTADAVRST"
     gene            complement(1287..1658)
                     /locus_tag="VFY80_02675"
     CDS             complement(1287..1658)
                     /locus_tag="VFY80_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6006837.1"
                     /translation="MARVLVVDDHLDAARMCQMLFESEGHQTRIAADGKAALELFESF
                     KPDFVFLDIALPGMDGYELAKTMRDRQQERRPNITALTGWGDLAATRLREAGIDSYFV
                     KPVSIAVLREVIAGQDKSYTV"
     gene            complement(1800..>2017)
                     /locus_tag="VFY80_02680"
     CDS             complement(1800..>2017)
                     /locus_tag="VFY80_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006838.1"
                     /translation="IEHPALIEAHGNHDCITGLRQRSLGLGHCGDERQLAIVETAASR
                     RLVSSIPAIRIRVLLILHPSYLSLLMI"
BASE COUNT          364 a          646 c          632 g          375 t
ORIGIN      
        1 cttgctcgat tacccgcttg agcgccgctc tgcgcgctga agcgcgccga tcccgtaata
       61 cgtcgcgtca ggcgtcgggc gcgcgcggcg gctcgatgcc tttttccgct ctgccgtcga
      121 gcaggtctgc cgcttcgtcc attcggcgcg cgatctcctc gcctccgcgt tgaatggcgt
      181 tacgcacttt cgccgcgtca tcgcgcagcg actgcgcgag gtgctttctg tccggcatgt
      241 acgcgtgttt ctcgggcacc attacgtcga tcgcacggca tccgccgtct gcggtaacgc
      301 ttcttttgaa acgcgatcca ctgcgcgcgc gacgcgttcg gcatgggagt aatgcagcat
      361 gtgacctgcg ccgtccaggg cttcgaattc gcagtgcggg agcgcgcgtg cgagacggga
      421 ggattggccg tcggctgcta cgatgccgtc ttcgtttccc gccattattg aaaccgggat
      481 atcacgcagt tcaccgtatc ggcgcgaaac cacgccggcg gtcgggacca tgcgggcagc
      541 gtcttccgca gaagcccgca actgctccgg ccgcagcatg atggaaaccg gcacggcatc
      601 gaggaataca tctggaacgg gttgcggcgc aaacatacgc cgtatcaggc gtggcgccag
      661 caggcgcgcc acaaaaggcg acatggtatg gcgcatgacc gtgccgagca cgggcatggc
      721 cggcgcgccg atcagcagcg aatcgatgcg tggcgacggg aaaaaatatc ccgacaccag
      781 caccagtccg tagacgcgcc ccgggtcgcc gagcgccatc tcgagcgcaa cgagcgtgcc
      841 ccatgagtga ccaacgacaa tggcgcgatc gatgccgagc gcgccgcacg cctcctgaag
      901 caattcggcc tgcgcgcgcg ccgaccatcc tttgcccggg cgttcgctgt agccgtatcc
      961 ggggcgatcg aacgcgatga cacggtagcg ttccgcgagg cggtccacga tcccgctcgc
     1021 gacataatcc tccgccgtca ccccggtgcc atggatcagc agcacgggca ctcccagacc
     1081 cttctcgatg tagtggagac gcacgccgca gacttcgatg aagcggccgg cgggcggatg
     1141 ctgcgcttca gcgcgtcggg tctgcgccct gacgagcagc gctgtcgccg ccaatccggc
     1201 ggccataccg acgagcgctg gaaggccggc gcctggccgt cgcatcttct tcggatcgaa
     1261 catagggctt cgtcggagtg agcgcgtcat accgtatagc tcttgtcctg accggctatc
     1321 acttcgcgca gcacggcgat actgaccggc ttgacgaaat aggaatcgat gccggcctcc
     1381 cgcaaccttg tcgcggcgag atccccccat cccgtcagcg ccgtaatgtt agggcgcctt
     1441 tcctgctgcc ggtcgcgcat ggtctttgcg agctcgtagc cgtccatgcc gggaagggcg
     1501 atgtcgagaa atacgaagtc cggcttgaac gattcgaaaa gctcgagggc ggcctttccg
     1561 tctgcggcga tccgggtctg gtgaccttcg gactcgaaga gcatctggca catgcgcgcc
     1621 gcgtccagat gatcatcgac gaccaaaacg cgggccatga cgcctccctg tgggttgagg
     1681 gaaccattat gttgtgcacg ctgttacacc gggaaccggc ctaccgttgc cgttcggact
     1741 agggcggcac gtcgcgtact gatgtcgtcg tctttcctac atacagggtg ccctgcaagt
     1801 catatcatca gcaagctgag ataagaggga tggagaatga gcagcacccg gatcctgatc
     1861 gccgggatcg aggagaccaa tcgacggctt gccgcagttt caacgattgc gagctgacgt
     1921 tcgtcgccac aatgtccgag gccaagactg cgctggcgca accctgtgat gcaatcgtga
     1981 ttgccgtgcg cttcgatgag agcaggatgt tcgatct
//
LOCUS       DASURG010000161         5527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1153698, whole genome shotgun sequence.
ACCESSION   DASURG010000161 DASURG010000000
VERSION     DASURG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153698"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1099)
                     /locus_tag="VFY80_02685"
     CDS             complement(128..1099)
                     /locus_tag="VFY80_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006839.1"
                     /translation="MRHALARTALICVAALVCVATSRAQDYPAKPVRIVVPLAPGGGN
                     DTTARLVAKKLADTMGQQVIVENRPGGAGAIAGEIVVRAPADGYTLYLASTTFTSSPA
                     LVRQLPFDTLRDFAPITRLAVVPGALIVHASLPVKSVKDVVALARSKPGEVTFGSAGM
                     GSGSHLGGELFKLLAHVNMTHVPYKGSAIVTTALLSGEVMTAFTNPISSLPHVKAGRL
                     KNLGVSSAERWPLLPDYPTIAESGVPGYETYIWNGMVVRTGTPAQIIQRLHKELTNAI
                     QAPDVAKHLAGDGSRPMVQTPEEFGAFLRNEVAKWDKVARAAGLRRD"
     gene            complement(1108..1899)
                     /locus_tag="VFY80_02690"
     CDS             complement(1108..1899)
                     /locus_tag="VFY80_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6006840.1"
                     /translation="MKLGLKGRKAFVTGASRGIGRSIAQAFAAEGIHLALFGRDLKRC
                     KAIADDLLERHERLKVVVVRLDFEEPKTVKPAVEKAARALGGVDILVNCAGGAYRGRL
                     TEIPDEMWERYFRVKPLGLVRMTRETLPYLKQSDQGRVINISGTRGREPEAHSMMSGP
                     INFGTLSLTKAMANELGPFGITVNAIAPGSTATRRWTELVNITARERDTTTEEAEKYL
                     LREVPLGKVITTEAIADLAVFLASARASLISGTAINVDGGRTRSI"
     gene            complement(1924..2943)
                     /locus_tag="VFY80_02695"
     CDS             complement(1924..2943)
                     /locus_tag="VFY80_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX6006841.1"
                     /translation="MPYTILYPDNRPSDMGIEKEVTGSDVVYINPCKTRFEDVEREAW
                     EKCDAIVVRRVHIDGSVIPHLKRTRIVVRMGVGFDVVDLEACGAAGIAVCNVPDYGTT
                     EVADSAIAMMLAFARGTAACDAALRADLKNSWTHLNNVTARRLRGATFGVIGFGRIGT
                     AAALRAQAFGMNVAFYDPYLPNGAELSFGYTRTRRLTDLLAMADVISIHAPLTKETRG
                     LINAAAVSGMKQGAYVINTARGPICDTAALLEGLKSGKLAAVGLDVLPKEPASLEDPL
                     VAAWRENEPWIRGRMLLNAHTGFYSPDALADMRRKSIETAYYYLRDGRLENCVNAEYL
                     KHRRP"
     gene            complement(2959..3417)
                     /locus_tag="VFY80_02700"
     CDS             complement(2959..3417)
                     /locus_tag="VFY80_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6006842.1"
                     /translation="MADAGPRTLFHFSFAVSDLERARRFYGEVLQCPEGRRLPGRADF
                     NFFGHHLVAHLAPGEVVGGQGAKVGDSKKTPLRHFGVVMTLEDFRTTADRLERCGVEF
                     LNEPSVTQKGTIREQMLMTVTDGCGNAIEFKGLNNITDVYAVERHEPAKA"
     gene            3552..4559
                     /locus_tag="VFY80_02705"
     CDS             3552..4559
                     /locus_tag="VFY80_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006843.1"
                     /translation="MLSTATSLARIARRITWWTACASLVAACSAPAMAAEAFPARPVR
                     MLIPFPAGGPADFVGRLFAQNLTDLWGRQVVADNRPGASGIIGTEIAIRSNPDGYTLL
                     FGSTSTFAVNLVLIRKLPYDVFRDLQLIGLVANAPHVLAVRSNLPAKDVKALIALAKK
                     QPGKFSFASAGPGTIVHMAGELFKYHAGIDILHVPFKGGAPATVGLLAGEVDMVVNDL
                     SAVLAHVRSGKLKAFGAAHTKRLQPLPDVPTFAELGMPGIVSSTWWGIAVPAQTPREV
                     VTRIKAAHDKVLARSDYIARLNELAMEPLVLTREQTSAFIRNEIEKWRKVAAAANVRI
                     D"
     gene            complement(4721..>5527)
                     /locus_tag="VFY80_02710"
     CDS             complement(4721..>5527)
                     /locus_tag="VFY80_02710"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6006844.1"
                     /translation="TMKASEAAEKTLTSGEVSWGGPLRVMKALDGNPAGGYVAFCEVV
                     QRDPFFLLGRAPNTRFTPADLAGKRVAVVSEVPTPWICLQYDLKRAGIDPGSVRCTPA
                     GTMAENAATLRAGEVDVIQVFQPYARELIDSRTGHIWYAAANRGLTTYTTFNSTRAFI
                     EKHPDVALRMTRAMYRTQKWIAAHDGRALAEAISAYLPDLPLATLAACCQDYKALGLW
                     NTQPVMRPEGFQWLRDAMLAARYIKTPLQFEQCVDLRFAEQVVREDPPSI"
BASE COUNT          949 a         1790 c         1784 g         1004 t
ORIGIN      
        1 cagctacggg accaacatgt ggagatgcgg tgacttgcaa aaaagcgcga atcgtttgcg
       61 gaagtagtgg cgcgagcacg cgcgctgggg caaggtcatc cgcgagaaca atatccgcgc
      121 cgaatgatca gtctctgcgc agccccgctg cgcgggcgac cttgtcccac ttcgcgactt
      181 cgttgcgcag aaacgcgccg aattcctcgg gcgtctgcac catcgggcgc gagccgtccc
      241 ctgcgaggtg tttcgcgaca tcgggcgcct ggatcgcgtt tgtcagctcc ttgtgcaggc
      301 gctggatgat ttgggccggc gtgccggttc tcaccaccat gccgttccag atgtaggttt
      361 cgtagcccgg cacgccggat tccgcaatcg tgggatagtc gggcaagagc ggccagcgtt
      421 ccgcgctcga cacgccgaga tttttcaggc gtcccgcctt cacgtgcggc agcgaggaga
      481 tcggattggt gaaagccgtc atcacctcac cggagagcag ggcggtggtg acgatggcgc
      541 tgcccttgta cggcacatgc gtcatattga cgtgcgcgag cagcttgaaa agttcgccgc
      601 cgagatggga gccgctgccc atgccggcgg agccgaacgt tacttctccg ggcttggaac
      661 gcgcgagcgc gacgacgtcc ttcaccgact tgacgggcag cgatgcgtgc acgatgagcg
      721 cgcccggcac gaccgcgagc cgagtgatcg gcgcgaaatc gcgcagcgtg tcgaatggaa
      781 gctggcgcac gagcgcggga ctcgaagtga aggtcgtgct ggcgagatag agcgtatagc
      841 cgtcggccgg cgcgcgcacc acgatttcac ccgcgatcgc ccccgcacca ccgggtcggt
      901 tctccacgat gacctgctgg cccatcgtgt cggcgagctt cttagcgacc aagcgggcag
      961 tcgtgtcgtt tccgccgccg ggtgcaagcg gcacgacgat gcgcaccggc tttgccggat
     1021 agtcctgcgc gcgggatgtc gccacgcaaa ccagtgctgc aacgcaaatc aatgctgtgc
     1081 gcgcgagggc gtgtctcatg ctgttcccta gatgctgcgc gtgcggccgc cgtccacgtt
     1141 gatcgcggtg ccgctgatga gactcgcgcg cgcggaggca agaaacaccg cgagatcggc
     1201 gatcgcttcg gtcgtgatca ctttgccgag cggcacctcg cgcaggagat acttctccgc
     1261 ctcttcggtt gtcgtgtccc gctcgcgagc ggtgatattg acgagctccg tccagcggcg
     1321 cgtcgcagtg gaccccggcg cgatggcgtt cacagtgatc ccgaatggtc cgagctcgtt
     1381 cgccatcgcc ttggtgagac tgagcgtccc gaagttgatc ggccccgaca tcatggaatg
     1441 cgcttccggc tcgcgaccgc gcgtgcccga gatgttgatg acacgaccct ggtccgactg
     1501 cttgagatac ggcagcgtct cgcgcgtcat gcgcacgagc cccaacggtt tcacacgaaa
     1561 atagcgctcc cacatctcgt ccggaatttc ggtgagccgc ccgcggtacg cgccgcctgc
     1621 gcagttcacc aggatatcga cgccgccgag cgcgcgtgcc gctttctcga ccgccggctt
     1681 caccgtcttc ggttcctcga agtcgagtcg cacgacaacc accttgagac gctcatggcg
     1741 ttcgagcaga tcgtccgcga tcgccttgca gcgcttgaga tcgcgcccaa aaagcgcgag
     1801 atgaatgcct tcggcggcga aggcctgggc gatgctgcgc ccgatgccgc ggctcgcgcc
     1861 ggtgacgaac gcctttctcc ccttgagacc caatttcatg tcgatcgact caggagtgcg
     1921 gcatcaaggg cggcggtgct tgagatactc cgcgttcacg cagttttcga gccggccgtc
     1981 gcgcagatag taataggcgg tctcgatcga tttgcgccgc atatcggcga gcgcgtcggg
     2041 gctgtagaag ccggtgtgtg cgttgagcag catgcggccg cggatccacg gctcgttctc
     2101 gcgccacgcg gcgaccagcg gatcctcgag tgaggccggc tcctttggga gcacatcgag
     2161 gcccacagcg gcgagcttgc cggacttcaa gccttcgagc agcgctgccg tgtcgcaaat
     2221 cgggccacgc gccgtattga tgacatacgc gccctgtttc ataccgctca cggcggccgc
     2281 gttgatgagt cctctcgttt ccttggtgag cggcgcatgg attgaaatca cgtccgccat
     2341 cgcgaggaga tcggtgagcc gccgtgtccg cgtatagccg aacgagagtt ccgcgccgtt
     2401 cggaaggtag ggatcgtaga acgctacgtt catgccgaac gcctgcgcgc gcagcgcggc
     2461 ggccgtgccg atgcgcccga acccgatcac gccgaacgtc gcgccgcgca gccggcgggc
     2521 ggtgacgttg ttcaaatggg tccacgaatt tttcagatcg gcgcgcaaag cggcatcgca
     2581 cgccgcggtc ccgcgcgcga aggcgagcat catcgcgatc gcggaatcgg cgacctcggt
     2641 tgtgccgtag tccggcacgt tgcagaccgc gatgccggcc gcgccgcacg cttcgagatc
     2701 gaccacatcg aagccgacgc ccatgcgcac cacgatacgg gtgcgcttca gatgcgggat
     2761 gacgctgcca tcgatgtgca cgcgccgcac gacaatcgcg tcgcacttct cccaagcctc
     2821 ccgctccacg tcttcgaaac gcgtcttgca ggggttgatg tagacgacgt cggaaccggt
     2881 cacctccttt tcgatgccca tatccgaggg ccggttgtcg ggatagagga tggtgtaggg
     2941 cacttccgct gctcctcttc aggccttcgc cggctcgtgc cgctccacgg cgtacacgtc
     3001 cgtgatgttg ttaaggccct tgaactcgat cgcgttgccg cagccgtccg tgacggtcat
     3061 cagcatctgt tcgcgaatcg tgcctttctg tgtcaccgaa ggctcgttca ggaattccac
     3121 gccgcagcgc tcgagccgat ctgcggtcgt gcgaaaatct tcgagcgtca tcacgacgcc
     3181 gaaatgccgg agcggcgtct tcttcgagtc cccgactttc gcgccctgac cgccgaccac
     3241 ttcgccgggt gcaaggtgcg ccacgaggtg atgaccgaag aaattgaaat cggcccggcc
     3301 cggcaatctc cttccctcgg ggcattgcag gacctcgccg tagaatctgc gcgcccgctc
     3361 gagatcgctt accgcgaagg aaaaatggaa cagcgtcctg ggtccggcgt ctgccatcgg
     3421 tagcctcatg caggaagggc ggggtggtcg atgctaccac gctcgccgcg ccgtgcaatg
     3481 cgcttgcctt gcggacgagc ggcgcagtgc gctaaaagta atgtgcccgc acaacaacaa
     3541 aggacaccct tatgctctcc acggccacct cgcttgcgcg catcgcccgg aggatcacat
     3601 ggtggaccgc gtgcgcgtcg ctcgttgcag cgtgcagcgc gcccgccatg gccgcggagg
     3661 cgtttccagc gcgtcccgtc cgcatgctca ttcctttccc ggccggcggg cctgcggatt
     3721 tcgtcgggcg cctcttcgcg cagaacctga ccgatctctg gggccggcag gtcgtcgcgg
     3781 acaaccgccc cggcgcgtcg ggcatcatcg gcaccgagat cgcgatccgc tcgaaccccg
     3841 acggctacac gctactcttc ggctcgacca gcaccttcgc tgtgaatctc gtgctgatca
     3901 ggaagctgcc ttacgacgta ttccgcgatc tgcagttgat cgggctcgtg gccaatgcgc
     3961 cgcacgtgct ggcggttcgc tccaatctgc cggcgaagga cgtgaaggcg ctgatcgcgc
     4021 tcgctaagaa acagccggga aaattcagtt tcgcttcggc cgggcccggc accatcgttc
     4081 acatggcggg cgagcttttc aagtatcacg ccggaatcga catcctccac gtgccgttca
     4141 agggcggcgc gcccgccacg gtcgggctgc tcgcgggtga ggtcgacatg gtggtgaacg
     4201 atctgtcggc cgtgctggcc catgtcagga gcggcaaatt gaaggcattc ggcgccgcgc
     4261 atacgaagcg gctgcagccg ctgccggatg tgccgacgtt cgccgagctc ggcatgccgg
     4321 ggatcgtatc gagcacctgg tggggaatcg ccgttccggc gcaaacgccg cgcgaagtcg
     4381 tgacgcgcat caaggcagcg cacgacaagg tgctcgcacg gagcgattac atcgcgcggc
     4441 tcaacgagct tgcgatggag ccgctggtgc tgacgcgcga gcagaccagc gcgttcatca
     4501 ggaacgagat cgagaaatgg cgcaaggtcg cggccgccgc caacgtccgc atcgattagc
     4561 gaaaccgtgc gtcatcgacc agcgacgagt aaacgctcgt cattccggtg aaaaccggaa
     4621 tccagcgtgg gctggatccc ggctccggat taaaccttcc ggcgatgctt accggcgccg
     4681 ggaagacaat gtgtttaatc gcgccggggc gatgatgcct ttatatcgac ggcggatcct
     4741 cgcgcacgac ctgctcggcg aaacgcaggt ccacgcactg ctcgaactga agtggcgtct
     4801 tgatatagcg cgcggcgagc attgcgtcac gcagccactg gaagccctcc ggccgcatca
     4861 ccggctgcgt gttccacagc ccgagcgcct tgtagtcctg gcagcacgcg gcgagcgtgg
     4921 cgagcggcag gtcgggaagg taggcggaaa tcgcttcggc gagcgcccgc ccgtcgtgcg
     4981 ccgcgatcca cttctgcgtg cggtacatcg cgcgcgtcat gcgaagcgcc acgtcgggat
     5041 gcttttcgat gaaggcgcgc gtgctattga atgtggtgta ggtcgtgagg ccgcgattcg
     5101 cggctgcgta ccagatatgg cctgtacggg aatcgatgag ctcgcgggcg tacggctgaa
     5161 acacctgaat cacgtccacc tcgccggcgc gcagcgttgc tgcattctcc gccatggtgc
     5221 cggcgggggt gcagcggacc gacccgggat cgatgccggc tcgcttgagg tcgtactgca
     5281 ggcagatcca cggcgtcggc acttcactca ccactgcgac acgcttgccg gcgagatctg
     5341 cgggcgtgaa tcgggtgttc ggcgcgcggc ccagcagaaa gaacgggtcg cgctgcacga
     5401 cttcgcaaaa cgcgacgtaa ccgccggcgg gattgccatc gagcgccttc atgacgcgca
     5461 gcggcccgcc ccaggacact tccccgctcg tcaatgtttt ttcggccgcc tccgacgctt
     5521 tcatggt
//
LOCUS       DASURG010000162         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_30529, whole genome shotgun sequence.
ACCESSION   DASURG010000162 DASURG010000000
VERSION     DASURG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30529"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFY80_02715"
     CDS             complement(<1..536)
                     /locus_tag="VFY80_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6006845.1"
                     /translation="MEALGPLEITFCVLVVAAGFAVRGTTGFGGNAIAIPLLTLILPI
                     QTVLAVMTVLTVLSSLGHWITDWRRIVWREIARVAPFMLIGVLAGLQLFQVLDTRTLT
                     RFFGIFVVVYALFAMATAKRTIAPPRKWLWPLATGLSALAGLVGTVFGGVAGPLYAIY
                     LNTLKLDKDRFRVTITTI"
     gene            complement(553..1566)
                     /locus_tag="VFY80_02720"
     CDS             complement(553..1566)
                     /locus_tag="VFY80_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6006846.1"
                     /translation="MEYRTLGASGLKVSRICLGAMMLGERTDNAVAVRILRSAREAGV
                     NFIDTADTYGEGESERIVGKFVARERDRWVIATKFASRLVADDPNSGGTSRKWVMQAV
                     EGSLKRLGTDYIDVYYFHRDDRATPLEESLAAMHDLIRTGKVRYFGLSNICGWRIAEV
                     VERCRALGAPRPIVLQPYYNALYRLAEVEQLPACRHYGIGVVPYSPLARGVLTGKYLP
                     GRKPPADSRAGRGEPRFMETEMRKESLAIAQVLKRHAERKGMTAAQFALNWTLNNAMV
                     SAVLAGPRTLEQWREYLGALRHRFDAEDEALVDRLVAPGHGSTPGYTDPKYPVTGRVR
                     VGA"
     gene            complement(1589..>2805)
                     /locus_tag="VFY80_02725"
     CDS             complement(1589..>2805)
                     /locus_tag="VFY80_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006847.1"
                     /translation="PSAAKRHVNVLSVVVATMVAQVASIMGVAVFPVIAPRLAAEMGL
                     SPVWVGYQVSLIYGSAMLAAPLLNAAIVRWGGCRATQAGLALNALGLAAALTSNLWAL
                     MATSILFGMALAVLVPASAHLLFRFCPPERRNLVFSLKQTGVPIGWTVMALAAPAITL
                     AYGWRWSIILVLLVVLATLAALQPVRARWDDDRNPQTAARQSALAGLVLTWRQPGLRR
                     LATASFFLSFVQLCLGSFAVTLLVQEAGYSLVTAGIMLSLVNVSGVAGRVLWGWYADR
                     MRSGTAVLWKLSMLVTLCCILTGFIAQSTPSFYIAVVFILFGATAVGWNGVYMAEIAR
                     LSPRGQVSIATSGARVWNFGGILIGPALFAAVYGRVGSYALTFAALAVISASAALLLA
                     SGRAARQNVAVH"
BASE COUNT          562 a          948 c          871 g          424 t
ORIGIN      
        1 aatatcgtgg tgatggtgac gcgaaaccgg tccttgtcga gcttgagcgt attgagatag
       61 atcgcgtaga gcggtccggc gacgccgccg aataccgtgc ccacgaggcc tgcgagggcg
      121 gaaaggccgg tcgcgagcgg ccacagccat tttcgcggcg gcgcgatcgt acgcttcgcc
      181 gtggccatgg cgaaaagcgc gtagacgacc acaaagatgc cgaagaagcg cgtcagcgtg
      241 cgcgtatcga gcacctggaa gagctgcagc cccgcgagca ccccgatcag catgaacggg
      301 gcaacccggg cgatttcgcg ccacacgatc cggcgccagt ccgtgatcca gtgcccgagc
      361 gaagaaagca cagtaagcac tgtcatcact gcgagaaccg tctgtatcgg caggatgagc
      421 gtaagcagtg gaatggcgat ggcgttgccg ccgaatccgg tggtaccgcg cacggcaaac
      481 ccggcggcca ccaccagcac gcaaaacgtg atctccagcg gcccgagcgc ttccacgagg
      541 caagggctta cgctatgcgc cgacccgaac gcggccggtg acggggtatt tgggatcggt
      601 atacccgggc gtcgaaccat ggccgggagc aaccagccga tcgaccagcg cctcgtcttc
      661 tgcgtcaaag cgatgacgca gtgcgccgag gtattcgcgc cactgctcga gcgtgcgcgg
      721 gccggccagc actgcactga ccattgcgtt attgagagtc cagttgagcg caaattgcgc
      781 cgccgtcatg ccttttcgct cggcgtgacg cttcaacacc tgcgcgatcg cgagcgactc
      841 cttgcgcatc tcggtttcca tgaaccgcgg ctcgccgcgc ccggcccgtg aatcggccgg
      901 cggctttctt ccgggcaggt atttacccgt gagcacgccg cgcgcaagcg ggctgtaggg
      961 caccacgccg attccgtagt gccggcacgc gggcaactgt tcgacctctg cgagccggta
     1021 gagcgcgttg tagtacggtt gaagaacgat cggtcgcggc gcgccgagcg cccggcagcg
     1081 ctccaccact tccgcgatgc gccagccgca gatattggag aggccgaaat agcgcacctt
     1141 gcccgtccgt atgagatcgt gcatggcggc gagcgactct tcgaggggtg tcgcacgatc
     1201 gtcgcgatgg aaatagtaga catcgatgta atccgtgccg aggcgcttca ggctgccctc
     1261 caccgcctgc atcacccact tgcggctcgt gcctccggaa ttcggatcat cggccactaa
     1321 cctgctcgcg aacttggtcg cgatcaccca gcggtcgcgc tccctggcga cgaacttccc
     1381 gacaatcctt tccgattcgc cctccccata ggtgtcggcg gtatcgatga agttcacccc
     1441 ggcctcgcgt gccgagcgca gaatgcgcac ggccacggca ttgtccgtgc gctcgccaag
     1501 catcatggcg ccgaggcaaa tgcgcgaaac cttaagcccg ctcgcgccga gcgtgcgata
     1561 ttccatgacg ttctccccaa ccggtcgatc agtggaccgc gacattctgc cgcgctgctc
     1621 tgccggatgc gagcagcagc gcggcgcttg ccgaaatgac ggcgagtgcc gcaaaggtga
     1681 gcgcatagct tccgacccgg ccgtataccg cggcaaaaag cgccgggccg atcagtatcc
     1741 cgccgaaatt ccacaccctg gcaccgctcg ttgcgatgct cacctgtccg cgcgggctca
     1801 accgcgcaat ctcggccatg tagacgccgt tccaccccac ggccgtcgca ccgaacagaa
     1861 tgaaaacgac ggcgatatag aagctcggtg tgctctgcgc gatgaagccg gtcaggatgc
     1921 agcagagggt cacgagcatg ctcaacttcc acagcacggc agttccgctc cgcatccggt
     1981 ccgcatacca gccccacagc acgcgccccg ccacgccgct cacgttgacg agggaaagca
     2041 tgatgccggc cgtcacgagc gagtagcccg cctcctgcac cagcagcgtc accgcaaacg
     2101 aaccgagaca gagttgcacg aacgacagaa agaatgacgc cgtcgcaagg cgccgcaatc
     2161 cgggctgccg ccacgtgagc accagtccgg cgagcgcgct ctgccttgca gccgtttgcg
     2221 gattgcggtc atcgtcccag cgcgcacgca cgggctgcag ggcggcgagc gttgcgagca
     2281 ctacgagcag cacgaggatg atcgaccacc gccacccata cgcaagggtg atcgcagggg
     2341 cggcgagcgc catcaccgtc cacccgatcg gcacgccggt ctgcttgagc gaaaaaacca
     2401 gattgcgccg ctccggcgga cagaatcgga acagcagatg ggccgatgcc ggcaccaaca
     2461 ccgcgagcgc catgccgaac aagatggacg tcgccatcaa cgcccagaga ttggacgtaa
     2521 gcgccgcggc cagccctagc gcgttgaggg cgagacccgc ttgcgtcgcg cggcagccgc
     2581 cccagcggac gatagcggca ttcagcaacg gcgcggcgag catcgccgat ccatagatca
     2641 ggctcacctg atagccgacc cataccggcg agaggcccat ctctgcggca aggcgcggcg
     2701 caatgacggg aaacacggcc acgcccatga tcgatgcaac ctgcgccacc atggtcgcca
     2761 cgacgacgct caatacgttg acgtgccgct tcgcagctga cgggt
//
LOCUS       DASURG010000163         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_643659, whole genome shotgun sequence.
ACCESSION   DASURG010000163 DASURG010000000
VERSION     DASURG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643659"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VFY80_02730"
     CDS             <1..1311
                     /locus_tag="VFY80_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX6006848.1"
                     /translation="VFNDLETTAHGSASIAQVHRARLADGAPVVLKVRRPGIRAKIDA
                     DLRILGHIAELIEAELPDARRYRPVEIAAEFSRSLERELDFTIEARHVERFAANFIGD
                     PYIVIPKVYPQWTSETLLVQEHVTGIPGTDYAAVEAAGLDRKVLMARGAECMLKMILI
                     DGFFHADPHPGNVFYLSGNRIAMIDYGMVGRLSAHRRRQVVDLLAGMARLEEEAMLEV
                     LIDWAGEAQVDEPRLIADVNELVFDYELMPLKDIRIGALIRQFAGIVRRHSIVLPTDL
                     TLMFKALITLEGLGRQYDPEFHISEHLTPWLRRALRQRYHPGEIIRRGRSAVNEVFSV
                     IGSVPRDLARLLREARRGRVRVDLDLKRLDSFGRQLDRTLDRATVGILTASLVIGSSI
                     VLTVRDSPSVFGIPVLPVIGLSGYMLAFLNSVWIIYGIWRSGKQ"
     gene            complement(1374..2429)
                     /locus_tag="VFY80_02735"
     CDS             complement(1374..2429)
                     /locus_tag="VFY80_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6006849.1"
                     /translation="MTYLAKPKLHHPTLPQNKLGYTRRDYEGKISTLCAGCGHDSISA
                     AIVQACWELDIEPHRVAKLSGIGCSSKTPDYFLGNSHGFNSVHGRMPSVMTGANLANR
                     ELIYLGVSGDGDSASIGLGQFAHCMRRGVNMVYIVENNGVYGLTKGQFSATADKGSKS
                     KRGVVNSDEPLDMIGMALLLGATFVARSFSGDKKQLVPLIKAAIEHKGAAFLDVISPC
                     VAFNNHPGSTKSYDYVRHHNEAVNRLDFIEGREEITADYAPGEVVNVRQHDGSVLRLR
                     KLAPDYDATNRIKVMNYLQERAAAGEVVTGLLYVDPEPADLHAHLNTVDTPLNRLDVR
                     ELCPGAVALERINASLR"
     gene            complement(2458..>3002)
                     /locus_tag="VFY80_02740"
     CDS             complement(2458..>3002)
                     /locus_tag="VFY80_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX6006850.1"
                     /translation="IAYRTYPGTHPTRGAFFTRGTTKDRYARYSEAGPDYVDNMQRLL
                     RKFETAKQLVPKPLLYPADKPARFGAIFYGSTSPAMQEALDVLAERGVHINALRVRGF
                     PFQEEISDFVASHPWVFVVEQNRDAQLKTLLVNEAKVNPTRLISVLHYDGTPITARFI
                     VGEIAKHAGARTVVPLKIAS"
BASE COUNT          492 a          942 c         1018 g          550 t
ORIGIN      
        1 gtcttcaacg acctggaaac cactgcgcat ggctcggcat ccatagcgca ggtccaccgt
       61 gcgcgcctgg ccgacggcgc gcccgtggtg ctcaaggtgc gccgccccgg gatacgcgcc
      121 aagatcgacg ccgacttgag aatcctgggg catatcgcag agctgatcga agcggagctg
      181 ccggacgcgc ggcgctaccg gccggtcgag atcgcggcgg aattctcgcg ctcgctcgaa
      241 cgcgagctcg acttcaccat cgaagcgcgc catgtcgagc gcttcgccgc gaacttcatc
      301 ggcgatccgt acatcgtcat cccgaaggtt tatccgcaat ggacgagcga gacgctgctc
      361 gtgcaggagc atgtgacggg cataccggga accgactacg ctgccgtcga agccgcgggt
      421 ctcgaccgga aagtgctgat ggcgcggggc gccgaatgca tgctgaagat gatcctgatc
      481 gacggcttct tccacgccga cccccatccc ggcaacgtgt tctacctttc cggcaaccgc
      541 atcgcgatga tcgattacgg catggtgggg cgcttgagcg cgcatcgccg caggcaggtc
      601 gtggaccttc ttgccgggat ggcgcgcctc gaggaagagg cgatgctcga agtgctgatc
      661 gactgggcgg gcgaggcgca ggtggacgag ccccggctga tcgctgacgt gaacgagctc
      721 gtattcgact acgagctgat gccgctcaag gacatccgga tcggggcgct catccggcag
      781 ttcgcgggga tcgtgcggag gcattccatc gtactgccca cggatctcac gctcatgttc
      841 aaggcgctca tcacgctcga agggctgggc cggcagtacg accccgaatt ccacatcagc
      901 gagcatctga cgccgtggct gcggcgcgcg ctccgccagc gttatcaccc gggcgagatc
      961 atccgtcgcg ggcgcagcgc ggtgaacgaa gttttcagcg tgatcgggag cgtgccgcgt
     1021 gatctcgcgc gcctgctgcg cgaggcgcgg cgcggccgag tgcgggtgga tctcgacctg
     1081 aagcggctcg attccttcgg ccggcagctc gaccggacgc tggaccgggc gaccgtgggg
     1141 atccttaccg cctcgctggt gatcgggtcg tccatcgtgc tcaccgtgcg cgacagcccc
     1201 tcggtgttcg gtatcccggt gctgccggtc atcgggctct ccggctacat gctggccttc
     1261 ctgaacagcg tgtggatcat ctacggcatc tggcggtccg gaaagcagta ggaccgcccc
     1321 gcctgccgtg cgagggaccg gcgggtgcgc gaaacgccgg gcggtgaatg cggctagcgc
     1381 agcgaggcgt tgatccgctc gagcgccacc gctccgggac atagctcgcg cacgtcgagc
     1441 ctgttgagcg gcgtgtcgac cgtgttgagg tgggcgtgca ggtcggccgg ctcgggatcg
     1501 acatagagca gccccgtcac gacttcgccc gccgcggcgc gctcctgcag gtagttcatc
     1561 accttgatgc ggttcgtcgc atcataatcg ggcgcgagct tcctcaggcg caggaccgag
     1621 ccatcgtgct ggcgcacgtt gacgacttcg ccgggcgcgt agtccgccgt gatttcttcc
     1681 ctgccctcga tgaaatcgag ccggttcacg gcttcgttgt gatggcggac gtagtcgtag
     1741 ctcttggtgg agccagggtg attgttgaaa gcgacgcacg gagagatcac atcgaggaat
     1801 gctgccccct tgtgctcgat cgcggccttg atgagcggca cgagctgttt cttgtccccg
     1861 gaaaagctgc gcgcgacgaa ggttgcgccg aggaggagcg ccatgccgat catgtcgagc
     1921 ggctcgtcgc tgttcaccac gccgcgcttg gacttcgagc ccttgtcggc ggtcgccgaa
     1981 aactggcctt tggtgagccc gtacacgccg ttgttctcga cgatatagac catgttgacg
     2041 ccgcggcgca tgcagtgcgc gaactgtccg agcccgatcg aagccgaatc gccgtcgccg
     2101 gaaaccccca ggtagatgag ctcgcgattg gcgagattgg cacccgtcat gacggagggc
     2161 atgcggccgt gcacgctgtt gaagccgtgc gaattgccga ggaaataatc cggtgtcttg
     2221 gagctgcagc cgatgccgga cagcttggcg acgcggtgcg gctcgatgtc gagctcccag
     2281 cacgcctgca cgatggcggc cgaaatggag tcgtgcccgc agcccgcgca cagcgtcgag
     2341 atcttgccct cgtagtcgcg gcgcgtgtag ccgagcttgt tctgcggcag cgtcgggtga
     2401 tgcagcttgg gcttggcgag atacgtcatc tgctattcct cgattcttga acgtgcgcta
     2461 cgacgcgatc ttgagcggga cgacggtgcg cgcgccggca tgcttggcga tttcacccac
     2521 gatgaagcgc gcggtgatcg gggtgccgtc atagtgcagc accgatatga gccgcgtcgg
     2581 attcaccttg gcctcgttca ccagcagcgt cttcagttgt gcgtcgcggt tctgctcgac
     2641 cacgaacacc cagggatgcg aggcgacgaa gtcgctgatc tcctcctgga acgggaagcc
     2701 gcgcacccgc agcgcgttga tgtgcacccc gcgctccgcg agaacgtcga gcgcttcctg
     2761 catcgccgga ctggtcgaac catagaagat cgcgccaaag cgcgccggct tgtccgcggg
     2821 gtagagcagc ggcttcggga cgagctgctt ggcggtttcg aacttgcgca gcagccgctg
     2881 catgttgtcg acgtagtccg gccccgcctc gctgtagcgg gcatagcgat ccttggtcgt
     2941 tccgcgcgtg aaaaaggcgc cgcgcgtcgg gtgcgtgccg ggataggtgc gatacgcgat
     3001 gc
//
LOCUS       DASURG010000164         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_745089, whole genome shotgun sequence.
ACCESSION   DASURG010000164 DASURG010000000
VERSION     DASURG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745089"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFY80_02745"
     CDS             <1..590
                     /locus_tag="VFY80_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX6006851.1"
                     /translation="AVMEKTPHVLLAGRAAERFAQRAGVPLSPLAYFTTPESVAALER
                     ARAREQKRVHGRASAADLHGTVGAVALDRHGNLAAATSTGGSTNKMAGRIGDSPLVGA
                     GLYADGTCAVSTTGLGEAFIRGVVAYDVAARMRYRDETLGRAARKALDRIVALGAEGG
                     LIAVDRAGRVAMPFVSEGMYRGMVRAGRFTTAVYR"
     gene            737..1960
                     /locus_tag="VFY80_02750"
     CDS             737..1960
                     /locus_tag="VFY80_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6006852.1"
                     /translation="MYNLHLTAEQLEFRDTLREFAEKEVRPVALHPDRLQPFEKPLLT
                     ELLDRASEMGLRTLGLSEEAGGAGADTLTACIVFEELAAGDVDIAAALGQTALIAGEL
                     FGSRMADAAREQLSKFVSDDKCHLAIAGAQQENLGWSYHRPLSGVAASLTATRQGADY
                     VINGTAPFVANAPIARLFVVVAGKSAFLVPRDTPGLSVGSTIRAIGGTAAGGEPGVRW
                     HHGPGASVAFKDCRVPVARSLGKEGEGLFASGVPAVRSDPVLAAINLGVGRAAYDAAV
                     EYAKIRRQGGRNIIEHQAIGGKLADCTMKLELSRTMIWKAAWAADHPDAVADRSLPAL
                     PLHAMARIYTAEAMHDVTLLAAECFGAMGVMRDMPLQKHVHDGMVFLHSEETDTASKL
                     QIAEAVAGYQRPAAA"
     gene            1978..>2187
                     /locus_tag="VFY80_02755"
     CDS             1978..>2187
                     /locus_tag="VFY80_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6006853.1"
                     /translation="MDFSLNEEQRHWQMEARKFAQDEIRPISLERDQIEGGFEPWDWE
                     IIEKGSKLGFRTLAVPKEWGGPGADF"
BASE COUNT          362 a          705 c          768 g          352 t
ORIGIN      
        1 gcgcggtgat ggagaagacg ccgcacgtcc tcctcgcggg gcgcgctgcg gagcgcttcg
       61 cacagcgcgc cggcgtaccg ctctcgccgc tcgcatactt cacgacgccc gagagcgtag
      121 cggctttgga gcgcgctcgc gcacgcgagc aaaagcgcgt gcatggacgc gcgagcgcgg
      181 cggacctcca cggcaccgtc ggcgcggtcg cgctcgaccg tcacggcaat cttgccgccg
      241 cgacctccac gggcggctcc accaacaaga tggcggggcg catcggggac tccccgttgg
      301 tcggtgcggg gctctacgcc gacggaacgt gcgccgtctc gacgaccggc ctcggcgaag
      361 cgttcattcg cggcgtcgtc gcctacgacg tcgcggcgcg catgcgctac cgcgacgaga
      421 cactcgggcg cgcggcgcgc aaggcgctcg accgcattgt ggcgctcggc gcggagggcg
      481 gcctgatcgc cgtcgatcgc gccgggcggg tcgcgatgcc cttcgtgagc gaaggcatgt
      541 accgcgggat ggtgcgcgcc gggcgcttca cgacggcggt gtatcgttga ctgggcagtc
      601 catatgctga gactttcctt tccttgcgtg cgctataccg ctatattggc gacctgaagt
      661 caccgtcaca tcgaaccact gcactgcgct cgaaacgccg ccaggcgctc cacttccgac
      721 aaccgggatt ctgcctatgt ataaccttca tctcactgcg gaacagctcg agttccgcga
      781 tacgctgcgc gagttcgccg agaaggaggt gaggccggtc gcgctgcatc cggaccgact
      841 ccagcccttc gaaaagccgc tgctcacgga actgctcgac cgggcgtccg aaatgggact
      901 ccgcacgctg ggactctccg aggaggccgg cggagcgggt gccgatacgc tgacggcgtg
      961 catcgtattt gaagaactcg cagcgggcga cgtggacatc gcggccgcgc tggggcagac
     1021 cgcactgatc gcgggcgagc tgttcggttc gcggatggcg gacgcggcgc gcgagcagct
     1081 ctcgaagttc gtttcggacg acaagtgcca tcttgccatc gccggcgcgc agcaggagaa
     1141 cctcggctgg tcgtatcacc ggcctttgag cggtgtcgcg gcgtcgctca ccgcgaccag
     1201 gcagggggcg gactatgtca tcaatggcac cgctccgttt gtcgccaatg caccgatcgc
     1261 caggcttttc gtggtcgttg ccggcaagag cgctttcctc gtgccgcgcg acacgccggg
     1321 actctctgtc ggcagcacga tccgtgcgat cggcggcacc gcggcgggcg gtgagccggg
     1381 cgtgcgatgg catcacgggc ccggcgcaag cgtcgcgttc aaggactgcc gcgtgcccgt
     1441 cgcgcgcagc ctcggcaagg aaggcgaggg cttattcgcg agcggtgtcc cggccgtgcg
     1501 gagcgatcct gttctcgcgg cgatcaatct gggtgtcggt cgggccgcct acgacgcggc
     1561 ggtggaatac gcgaaaatcc gccgccaggg cgggcgcaac atcatcgaac accaggcaat
     1621 cggcggcaag cttgccgact gtacgatgaa gctcgagttg tcgcgcacca tgatctggaa
     1681 agcggcatgg gcggcggatc accccgatgc ggtggccgat cgcagcctgc ctgcgctgcc
     1741 gctgcacgcg atggcgcgca tctataccgc ggaagcgatg cacgatgtga cgttgctggc
     1801 ggcggaatgc ttcggcgcga tgggtgtgat gcgcgacatg ccgcttcaga agcacgtgca
     1861 cgacggcatg gtattcctcc actcggaaga aacggacacc gcttcgaaat tgcagatcgc
     1921 agaggcagtc gcgggctatc agcgcccggc ggcggcgtaa cggtaaggac caagcgtatg
     1981 gatttttcgc tgaacgaaga gcagcgccac tggcagatgg aggcgcgcaa gtttgcgcag
     2041 gacgagatcc gcccgatctc gctcgagcgc gaccagatcg aaggcggctt cgagccgtgg
     2101 gactgggaga tcatcgagaa gggctcgaag ctcggtttcc ggaccctcgc cgtgccgaag
     2161 gagtggggcg ggccgggcgc cgacttc
//
LOCUS       DASURG010000165         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_950132, whole genome shotgun sequence.
ACCESSION   DASURG010000165 DASURG010000000
VERSION     DASURG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950132"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..757
                     /locus_tag="VFY80_02760"
     CDS             113..757
                     /locus_tag="VFY80_02760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6006854.1"
                     /translation="MDSNKIERVYTGYAKVYNRIFGTIFQRSREAAIRDLDVQPGERV
                     LEVGVGTGLCLPLYPKNCQITGVDLSDGMLDRARELVRERHLTHVRLFRMDAGALDFP
                     DGSFDTVVAAYVVTAVPDHRKVMSEMIRVCRPGGRIMLLNHFTNDSKLIAAVEKAISP
                     LCKHIGFRTDLSVADVLDGWPVIMHRHKRLNPLQMWHFVECINAKAPEPDPAVR"
     gene            complement(789..>2372)
                     /gene="typA"
                     /locus_tag="VFY80_02765"
     CDS             complement(789..>2372)
                     /gene="typA"
                     /locus_tag="VFY80_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX6006855.1"
                     /translation="FGGEVERVLSMVDGVLLLVDAVDGPMPQTRFVTRKALAQGLKPI
                     VVVNKVDRPGARPEWVVNQTFDLFDKLGANEEQLDFPVVYASALEGWATLSLDGTSPH
                     ADMRPLFEAILQQVPVRADDIDGPLQLQICSLDYSSYVGRIGIGRVNRGRIRPGQIVA
                     VLRGPQSAPAMSKVNQVLVFKGLERVLAEEAMAGDIVLVNGIEEVGIGVTLADPEHPE
                     ALPLLKVDEPTLTMNFQVNTSPFAGREGRFVTSRQLRERLVRETMSNVALRVEDTADA
                     DVFLVSGRGELHLTILLENMRREGYELAVSRPRVVTREIDGVRCEPFELLAVDVEEVN
                     QGALMEALGARRGELLDMQPDGKGRVRLDYRIPARGLIGFQSEFMTLTRGTGIMSHVF
                     DDYAPMKGDIAERRNGVLISAEDGEAVAYALWKLQERGRMFVNPGDPLYEGMVIGIHT
                     RDNDLVVNPVKTKQLTNIRAAGKDEAILLTPPIRHTLESAIEFIADDELVEITPKSIR
                     IRKRVLQEQERKRASRAAA"
BASE COUNT          412 a          790 c          736 g          434 t
ORIGIN      
        1 gtacgcacat ttggaagacg gttccatcct cgaaaaccgc cttgccgcat gccgaccggg
       61 gtatcccacg gttttgccgt aagccgaacg ttcaataatc ccgtattcat ccatggactc
      121 gaacaaaatc gaacgcgtct acaccggcta tgcaaaggtc tacaaccgta ttttcggcac
      181 catcttccag cgttcgcgcg aggccgccat acgcgatctc gacgtgcagc cgggcgagcg
      241 ggtgctcgaa gtgggtgtgg gaacgggttt gtgcctgccg ctgtatccga agaactgcca
      301 gattaccggc gtcgatctct cggacggcat gctggacagg gcgcgcgagc tggtacgcga
      361 gcgccatctg acgcacgtca ggctcttccg catggatgcg ggggcgctcg attttcccga
      421 tggcagcttc gataccgtgg tggcggccta cgtcgtgacc gccgtccctg accaccgtaa
      481 ggtaatgagt gaaatgattc gcgtttgccg gcccggcggg cggatcatgc tcctcaatca
      541 cttcaccaat gacagcaagc tcatcgcggc cgtggaaaag gcgatttcgc cgctttgcaa
      601 acacatcggc tttcgcaccg acctttcggt cgccgacgtg ctcgacggct ggccggtcat
      661 catgcaccgc cacaaacggc tgaatccgct gcagatgtgg cacttcgtcg agtgcatcaa
      721 cgcgaaggcg cccgagccgg atccggcggt ccggtagtcc ccttctgcgc tttccggcaa
      781 cgcccgaatc aggctgccgc gcgtgaggcg cgcttccgct cctgctcctg cagcacgcgc
      841 ttgcggatgc ggatcgactt cggcgtgatt tccaccagct cgtcgtcggc gatgaactcg
      901 atggcggact cgagcgtgtg gcgaatcggc ggcgtgagca ggatcgcttc atccttgccg
      961 gccgcgcgga tgttggtcag ctgcttggtt tttaccgggt tcacgacgag gtcgttgtcg
     1021 cgggtatgga tgccgatcac catgccttcg tagagcggat cgcccggatt cacgaacatg
     1081 cgcccgcgct cctgcagctt ccacagcgcg taggcgactg cttcaccgtc ctccgctgaa
     1141 atcagcacgc cgttgcgccg ctcggcgatg tcgcccttca tcggggcgta gtcgtcgaac
     1201 acgtggctca tgatgccggt gccgcgcgtc aacgtcatga actccgactg gaagccgatc
     1261 aggccgcgcg ccgggatgcg gtagtcgagc cgcacgcgcc ccttgccgtc gggctgcatg
     1321 tcgaggagct cgccgcgccg cgcgccgagc gcttccatga gcgcgccctg gttcacctcc
     1381 tcaacgtcga cggccaatag ctcgaatggc tcgcagcgca caccgtcgat ctcgcgcgtc
     1441 accacacgcg ggcgtgagac cgcaagctcg tagccttcgc ggcgcatgtt ctcgagcagg
     1501 atcgtgagat gcagctcccc gcgacccgag acgagaaaca cgtccgcatc ggcggtatcc
     1561 tccacccgca acgcgacgtt gctcatggtc tcgcgcacga gccgctcgcg cagctgccgg
     1621 ctggtgacga aacgcccttc gcggccggcg aacggagaag tgttcacctg gaaattcatg
     1681 gtgagcgtgg gctcatccac cttgaggagc ggcagcgctt ccgggtgctc gggatcggca
     1741 agcgtcacgc cgatgccgac ttcctcgatg ccgttcacga gcacgatgtc gcccgccatg
     1801 gcttcttccg ccaacacgcg ctcgaggccc ttgaagacga gcacctgatt gaccttcgac
     1861 atggcgggcg cgctctgcgg cccgcgcaac acggcgacga tctggccggg gcggatgcgg
     1921 ccgcggttga cgcgaccaat gccgatgcgt ccgacgtagc tcgagtagtc gagcgagcag
     1981 atctgaagct gcagcggacc gtcgatgtcg tccgcgcgca cgggcacctg ctgcaggatc
     2041 gcctcgaaga gcggccgcat gtcggcgtgc ggcgatgtgc catccaggct caatgtcgcc
     2101 cacccttcga gcgcggaagc gtacaccacg ggaaagtcga gctgctcctc gttggcgccg
     2161 agcttgtcga agagatcgaa cgtctgattg acgacccact cgggtcgagc gcccggccgg
     2221 tccaccttgt ttacgacgac aatgggcttc aggccttgcg ccagcgcctt gcgcgtgacg
     2281 aaacgcgttt gcggcatcgg cccgtccaca gcatcgacga gcagcagcac gccgtccacc
     2341 atcgaaagca cccgctcgac ctcgccgccg aa
//
LOCUS       DASURG010000166         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_235377, whole genome shotgun sequence.
ACCESSION   DASURG010000166 DASURG010000000
VERSION     DASURG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235377"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFY80_02770"
     CDS             complement(<1..414)
                     /locus_tag="VFY80_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006856.1"
                     /translation="MSNNTAVVAALPLALLQSTRSHDRPGEVLEDEDLSLSLPRRLGL
                     SGVIERQIQQYETAAKSGKRVSGEEFHNLLKLVLRRPDAELILRETGARVAQRQFSSV
                     SGAWLKLLRLMPRTAMLHAIRTATTKLLRNMSGAAE"
     gene            507..947
                     /locus_tag="VFY80_02775"
     CDS             507..947
                     /locus_tag="VFY80_02775"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6006857.1"
                     /translation="MEESCIFCRIIGGEEMVSVVYEDNLAIAFLDVQPVSQGHTLVVS
                     KQHYETLFDVPESLLTHCMAVAKRIAPGLRKAVGAHAINVFSANGKAGGQDVPHFHMH
                     LIPIREGEPFALQLPPDDARIPMRAELDITAARIGRAIQETKVE"
     gene            947..2068
                     /locus_tag="VFY80_02780"
     CDS             947..2068
                     /locus_tag="VFY80_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6006858.1"
                     /translation="MRRYQPFDPPEYLSWAPDAAVLREFVQTIERDAERARLIGALSD
                     AQLLRLYEGLIRFRLHDYALKRWVRQGVISKAWLGTGEEAITIGTVHALERDRDIVAP
                     MIRNAGACHEMGMSIEQMLHGYLGTDQSPNHGKDAHIGDLRIGVLEPISHVGDSVPVI
                     TGIALAFKSRQEPRVALTWIGDGSTKTAAVHEGLNLAAVLKVPAIFVIQNNQVALGTR
                     VQQHQAGDFAAWGRMYGVPLLVVDGNHVLDVYAATRIAADRARSGGGPSIMLANTFRM
                     GGHATHDEAEARRTFAPELFAHWGKRDPIGMYEEYLTQRSVTREKLEEIEARVIAEVD
                     AAAESAAAARTTALPAAQAAELSGISAGMRQPGLAHRLG"
     gene            2068..>2412
                     /locus_tag="VFY80_02785"
     CDS             2068..>2412
                     /locus_tag="VFY80_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6006859.1"
                     /translation="MIDDVRFVELRRAFEQHAPVPAERYEGAREAAVALLLRAHDELE
                     LLLIKRAEQESDPWSGHVALPGGRRNRHDSDLLHTALRETEEEVGIAVGRDLQLIGAL
                     DEVSPRNPRLPPL"
BASE COUNT          464 a          730 c          789 g          429 t
ORIGIN      
        1 ttccgcagca ccgctcatgt tgcgcaacaa cttcgtggtc gcggtgcgaa tggcatgcag
       61 catggccgtg cgcggcatga ggcgcagcag cttcagccac gcaccggaca cactcgagaa
      121 ttgacgttgc gcgacgcgtg caccggtttc gcgcagaatc agctccgcgt ccgggcgccg
      181 caacaccagt ttgagcaggt tgtgaaattc ctcgccggaa acgcgcttgc ccgacttggc
      241 cgctgtctca tactgctgga tctggcgttc gatgacgccg ctcaacccca gacggcgcgg
      301 caggctgagt gatagatcct cgtcttcgag gacttcgccg ggccggtcgt gcgagcgcgt
      361 cgattgtaat aatgcaagcg gcagggccgc gacaactgca gtgttgttac tcataattgt
      421 accggcaaat ttaaccctgg gcctgcaagg tgcaatcgga ctcggcagcc tttactttca
      481 gcaaggaata aacccgggag aacggtgtgg aggagtcctg tattttctgc cgcattatag
      541 gcggcgaaga aatggtcagc gtcgtctacg aagataatct cgccatcgcc tttctggacg
      601 tgcagccggt aagccagggg cataccctgg tcgtctccaa gcagcactat gagacgcttt
      661 tcgacgtgcc cgagtcactt ctgacccact gcatggcagt ggccaaacgc attgcgcccg
      721 ggctgcgcaa ggcggtcggc gcgcacgcca tcaatgtttt cagcgccaac ggcaaggcgg
      781 gtggacagga tgtcccgcat ttccacatgc atctgattcc gattcgggaa ggcgagccgt
      841 tcgcgctcca gctgcccccg gacgacgcgc gcatacccat gcgagcggag ctggacatca
      901 cggcagcgcg catcggccgg gcgattcagg aaacaaaggt cgagtaatgc gtcggtatca
      961 gcctttcgac cccccggaat acctgagctg ggcgcccgat gcggccgtgc tgcgggagtt
     1021 cgtgcagacc atcgagcgtg atgcggaacg cgcgcgtttg atcggcgcgc tctccgacgc
     1081 gcagctgctg cgtctgtacg aaggactgat acgcttccgg ttgcatgatt acgcgctcaa
     1141 gcgctgggta cggcaggggg tgatcagcaa ggcgtggctg ggcacgggcg aagaggcgat
     1201 cacgatcggc accgtgcacg cgttggaacg ggatcgggat atcgtcgcgc ccatgatccg
     1261 caacgccggc gcctgccatg agatgggcat gtcgatcgag cagatgttgc acggctacct
     1321 gggaacggat caaagtccga accacggcaa ggatgcgcac atcggcgatc tgcgcatagg
     1381 cgtgctggag ccgatttcgc acgtgggcga cagcgttccc gtcatcaccg ggattgcgct
     1441 cgcgttcaag agcaggcagg agccgcgcgt tgccctcacc tggatcggcg atggctccac
     1501 caaaaccgcg gccgtgcacg agggcttgaa tctggcggcg gtgctgaaag ttcctgcaat
     1561 cttcgtgatt caaaacaatc aggttgcgct gggtacgcgc gtgcaacagc accaggcggg
     1621 cgattttgcg gcatggggac gcatgtacgg tgtgcccctg ctggtggtgg atgggaacca
     1681 cgtgctggat gtgtatgccg cgaccagaat cgccgcggat cgtgcacggt ccggcggcgg
     1741 cccatccatc atgctcgcca atacctttcg catgggcggg catgctacgc acgacgaagc
     1801 agaagcacgc cgtacctttg cgcccgagct gtttgcgcat tggggaaagc gcgatcccat
     1861 cggcatgtac gaagagtatc taacgcagcg cagcgtcacg cgggagaagc tggaggagat
     1921 cgaagcgcgc gtcatcgccg aagtcgatgc ggccgccgag agcgcagcgg ctgcgcgcac
     1981 cacggcgctg cccgcagcgc aggcggccga gttgagcggt atcagcgcgg gcatgcggca
     2041 acccggactg gcgcacagac taggttgatg attgacgacg tacgtttcgt cgagcttcgt
     2101 cgcgccttcg aacaacacgc gcccgtcccg gccgagcgtt atgaaggtgc acgcgaggcg
     2161 gcagttgctc tcctgctgcg cgcccacgat gagctcgagc tgctgctgat caagcgcgcc
     2221 gagcaggaat ccgatccctg gtcggggcat gtagcgctac ctggcggacg acgcaaccgg
     2281 cacgactccg atctgctgca cacggcgctg cgggaaacgg aggaagaggt cggcattgcg
     2341 gtcgggcgcg acctgcagct cattggcgcc ctcgatgaag tgagtccgcg caacccacgg
     2401 ctccctccac tc
//
LOCUS       DASURG010000167         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1154262, whole genome shotgun sequence.
ACCESSION   DASURG010000167 DASURG010000000
VERSION     DASURG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154262"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1511
                     /locus_tag="VFY80_02790"
     CDS             336..1511
                     /locus_tag="VFY80_02790"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX6006860.1"
                     /translation="MDGSATQHSVGIVAPQIAKFTEPVTLKCGAVIDEYELMYETYGA
                     LNAERSNAVLVCHALNASHHVAGIYADDSANVGWWDNMIGPGKPVDTSRFFVVGVNNL
                     GGCHGSTGPRSIHPATGRPWGSAFPVVTVEDWVATQARLADHLGIRRFAAVMGGSLGA
                     MQALAWTVMFPERIRHSVVIACTPNLSAQNIAFNEVARQAIITDPDFYGGDYYAHGTT
                     PKRGLRVARMVGHITYLSDDEMASKFGRQLKRGKLQYSFDTEFEIESYLRYQGNKFAE
                     YFDANTYLRITKVLDYFDPALEHGGDLARALAPARASFLVVSFTTDWRFSPERSREIV
                     TALLGNRRDVTYAEIDAPHGHDAFLLDDARYHRLVRAYFDRIAAEAGIPGAPTGLPA"
     gene            1508..2113
                     /gene="metW"
                     /locus_tag="VFY80_02795"
     CDS             1508..2113
                     /gene="metW"
                     /locus_tag="VFY80_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX6006861.1"
                     /translation="MRANGNGIQRSDFAAIAEWVRPGSRVLDLGCGDGTLLKYLSSQR
                     GVTGYGVEIADEKVLACVKNGVNVIQGDLERGLTEFEDQSFDYVILSQTLQAVKNSER
                     IIRDMLRVGREGIVTFPNFGYWKNRLHVLSGYMPVSENLPYQWFDTPNVHLCTIADFE
                     RFCRERRIRVVERKVLTDGATVKLLPNLLGALAIYHFGTSA"
BASE COUNT          418 a          698 c          685 g          405 t
ORIGIN      
        1 ctcgcgctgg gactgcgtca gtattccatg catgccgcgc atctgctcga tgtgaagcag
       61 cacattctca agtcgagcct gcagcatatc cggccgattg cgctcaaaat gctgcgcacc
      121 gaggatcccg agaagctgcg gaggctgctc gaccgcgtga acgcctagtg cgttggcatt
      181 tgacaaggcg cgggggccgc tgtaacgtga cctcccgttt tcgcgccgat ttgataatca
      241 gcttgcgggc gcgtaataaa tccggctaaa gagacgcttt aggccgcaca gacttctcag
      301 aaccccggga cgcaagccgt gaccggggtt tttttatgga cggaagcgcc acacagcatt
      361 cggtcggcat cgtcgcgccg cagatcgcga agttcaccga acccgtgaca ttgaaatgcg
      421 gggcggtgat cgacgaatac gagctcatgt acgagaccta tggcgcgctc aatgccgagc
      481 gctcgaacgc cgtgctcgta tgccacgcgc tcaatgcctc ccaccacgtc gccggcatct
      541 atgcggacga ttcggcgaat gtcggctggt gggacaacat gatcggtccc gggaagccgg
      601 tggacaccag ccgctttttc gtggtgggcg tcaacaacct cggcggttgc cacggctcga
      661 ccggcccgcg gagcatccat cccgcaaccg gcagaccgtg gggaagcgct tttccggtcg
      721 taacggttga ggactgggtc gcaacgcagg ctcggctcgc cgatcacctc ggcatacgcc
      781 ggttcgcggc ggtcatgggc ggcagcctcg gtgccatgca ggcgctcgcc tggaccgtca
      841 tgtttccgga gcgcatacgg cactcggtcg tcatcgcgtg cacgccgaat ctctccgcac
      901 agaacatcgc gttcaacgaa gtcgcgcgcc aggcgatcat caccgatccc gatttctacg
      961 gtggcgacta ctacgcgcac ggcacaacgc ccaagcgcgg gctgcgggtc gcgcgcatgg
     1021 tgggtcacat cacctatctg tccgacgacg agatggcgag caagttcggg cggcagttga
     1081 agcgcgggaa actccagtat tccttcgaca ccgagttcga aatcgagtct tacctgcgct
     1141 accagggaaa caagttcgcg gagtacttcg atgccaacac gtatttgcgc atcaccaagg
     1201 tgctcgacta tttcgatccc gcgctcgagc acggcggcga ccttgcacgc gcgctcgcgc
     1261 cggcgagggc gagcttcctc gtcgtttcgt tcaccaccga ctggcgcttc tcgccggaac
     1321 gctcacggga aatcgtgact gcgctgctcg gcaaccgccg cgatgtgacc tacgccgaga
     1381 tcgacgcgcc gcacggccac gacgcctttc tcctcgacga cgcgcgatat caccgactgg
     1441 tgagggcgta cttcgaccgc attgccgccg aggccgggat accgggtgcg ccgacgggac
     1501 tgcccgcgtg agagcgaacg gcaacggcat ccagcgttcc gactttgcgg ccatcgcgga
     1561 gtgggtgcgt ccgggctcgc gcgttctcga cctgggatgc ggtgacggca cgctgctcaa
     1621 gtacttgagc tcacagcgcg gggtaaccgg ctacggcgtc gagattgcgg atgagaaagt
     1681 gctcgcgtgc gtgaagaacg gcgtcaacgt gatccaggga gatctcgagc gcggcttgac
     1741 cgagttcgag gatcagtctt tcgactacgt gatcctgtcg cagacgcttc aggccgtgaa
     1801 gaacagcgag cgcatcatcc gggacatgct gcgggtaggc cgtgaaggta tcgtcacctt
     1861 ccccaacttc ggttactgga agaaccggct gcacgtcctc tcgggctaca tgccggtatc
     1921 cgaaaacctg ccctaccagt ggttcgatac gcccaacgtg catctctgca ccattgccga
     1981 tttcgagcgc ttctgccgcg agcggcgcat ccgggtcgtc gagcgcaagg tgcttaccga
     2041 cggcgcgacg gtaaagttgc tgcccaatct attgggcgcg ctggcgatct accacttcgg
     2101 cacgagcgct tgacggcctg ccctcgtcct ttccggccga gccgatggag acgtcagcga
     2161 ggcctgagcg catcatgatc cagaaaagaa ccacgccggg aaaagc
//
LOCUS       DASURG010000168         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_644072, whole genome shotgun sequence.
ACCESSION   DASURG010000168 DASURG010000000
VERSION     DASURG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644072"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VFY80_02800"
     CDS             complement(<1..1177)
                     /locus_tag="VFY80_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX6006862.1"
                     /translation="MAAFAERSYLTYAMSVVRGRAIPNVEDGQKPVQRRILYAMRELN
                     LRPGVQHSKSARIVGEVLGKYHPHGDVSTYDALARMAQDFSLRYPLIDGQGNFGTADG
                     DAPAAYRYTEARLTPIAELLLSEIAQDTVDFIPNYDARLTEPELLPARLPMLLLNGAS
                     GVAVGMACELPPHNLREVAEAAIALIRHPKTKTAALMDIIKGPDFPGGGQIISSRQAI
                     LEAYETGRGSLRARCRWKVENLARGQWQVVVYELPPTTSAQRVRAQIEDLTNPKPKER
                     GGKVTAAQANLKQLIVGALEKVNDESDKENKVRLVFEPRSSRQDPEEFMRLMLAHTSL
                     EENFPINLVVLGLDGRPGRKSIGQILEEWVTFRLATVTRRTKFRLSQVERRIHILEGR
                     "
     gene            complement(1303..>2941)
                     /locus_tag="VFY80_02805"
     CDS             complement(1303..>2941)
                     /locus_tag="VFY80_02805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX6006863.1"
                     /translation="AYKFSGGLHGVGVSVTNALSERLEVEVRRDGQIHAMGFANGAVA
                     QKLKVKGKCPRNDTGTALRIWPNAKYFDTPKVSLPDLERIVRSKAVLLPGVKVTLNIE
                     GAKETTSRTWSYPEGMKGYLEELLAEAEPVAPIFAGEKYVQEANGFAAGEGAGWAFAF
                     VEAGKGYGESYANLIPTPAGGTHEAGLRDGIFDAIRAFAEHHNMMPRGIRLQADDAWS
                     RTVYFLSAKVLEPQFHGQTKEKLSNRDALKLVSVMVRDPFELWLNTHVDYGRKIAELA
                     IRAAVERSRTVQKVERRKSSSVVMLPEKLTDCESSELAENELFLVEGDSAGGSAKQAR
                     DKRFQAIYKMRGKALNSWEVKRELLFSNAEIHDIAVAIGVDPHAEGDEPDLSGLRYGK
                     IASLTDADVDGAHISVLLLTLFYRHFPRLIERGHIYVACPPLYKVDVPAQGKRPAKKV
                     YALDDEERKTILDRARDDGIRVESLSIQRFKGLGEMNPAQLWETTLCPDTRRLLQVKI
                     EDREKTYTLFNMLMSRREAESRCAWMEEKGDQVEADV"
BASE COUNT          471 a          971 c          923 g          576 t
ORIGIN      
        1 gccggccttc caggatgtgg atgcgccgct cgacctgcga gagccggaat ttcgtgcgcc
       61 gcgtgaccgt cgccagccgg aacgttaccc actcctcgag gatctgcccg atgctcttgc
      121 gtccgggacg cccatcgaga ccgagcacca cgagattgat ggggaagttt tcctcgaggc
      181 ttgtgtgcgc cagcatgagg cgcatgaatt cctcgggatc ctggcgcgag gagcgcggct
      241 cgaaaacgag ccgcaccttg ttctccttgt ccgactcgtc gttgaccttc tccagtgcac
      301 cgacgatgag ctgcttcaga ttggcctgag cggcggtgac cttgccgccg cgctctttcg
      361 gtttgggatt ggtcaagtcc tcgatctgtg cccgcacgcg ctgcgcggac gtagtgggcg
      421 gcagctcgta cacgaccacc tgccattgcc cgcgcgcgag gttctccact ttccagcggc
      481 agcgtgcgcg gagcgatccg cgcccggtct cataggcttc gaggatcgcc tgccgcgagc
      541 tgatgatctg gccgccgccg gggaagtccg gccccttgat gatgtccatc agcgctgccg
      601 ttttggtctt ggggtggcgg atcaacgcga tcgcagcctc cgccacctcg cgcaggttgt
      661 gcggcggcag ttcgcacgcc atgccgaccg caacgccgga agcgccgttc aggagcagca
      721 tcggcagccg cgccggaagc agttcgggtt cggtcaggcg tgcgtcatag ttcggaatga
      781 aatcgacggt gtcctgggcg atttcggaga gcaggagttc cgcgatcgga gtcagccgcg
      841 cctcggtata gcggtaggcc gccggcgcat cgccgtccgc cgtgccgaag ttgccctgcc
      901 cgtcgatcag cggatagcgc aggctgaagt cctgcgccat ccgcgcgagc gcgtcgtaag
      961 tcgacacgtc gccgtgcggg tgatacttgc cgagcacttc gcctacgatg cgcgcggact
     1021 tggaatgctg cacgccgggc cggagattca actcgcgcat tgcatagaga atgcgccgct
     1081 gcaccggctt ctggccgtct tcgacgttcg gaatggcgcg accgcgcacc acactcatcg
     1141 cataggtgag gtagctccgc tcggcgaacg ccgccatgtc gatgtagtca tcgccgccga
     1201 ctgcggtcgg aggcggtggc gcaccgcccg ctccgccgcc gccttcgaag agatcgccgg
     1261 tcgcctcgtc cttcgcccgc cctcgcgtct tggtatccgt cgtcatacgt cagcttccac
     1321 ctggtcgccc ttctcttcca tccacgcgca gcggctctct gcctcgcggc gcgacatcag
     1381 catgttgaat agcgtgtaag tcttctcgcg atcctcgatc ttgacctgca gcagacgccg
     1441 cgtatcgggg cacagggtgg tctcccatag ctgcgcggga ttcatttcgc cgagaccctt
     1501 gaagcgctgg atggacagcg actccacgcg gatgccgtcg tcgcgcgcgc ggtcgagaat
     1561 ggtcttccgc tcctcgtcgt cgagcgcgta aacctttttt gccggccgct taccctgagc
     1621 cggcacgtcg accttgtaga gcggcgggca ggcgacgtag atgtggccgc gctcgatgag
     1681 tctggggaaa tggcgataga agagcgtgag cagcagcacc gagatatgcg caccatcgac
     1741 gtcggcgtcc gtcagcgagg cgatctttcc gtagcgtaaa ccggagagat cgggctcgtc
     1801 gccttcggcg tgcggatcga cgccgatcgc caccgcgatg tcgtggattt ccgcgttgga
     1861 gaaaaggagc tcccgcttca cttcccacga attgagcgcc ttgccgcgca tcttgtagat
     1921 cgcctggaag cgcttgtcgc gcgcctgctt cgcggagccg ccggccgagt cgccctccac
     1981 cagaaaaagc tcattttcgg cgagctcgct cgactcgcag tcggtcaact tctccggcag
     2041 catcacgacg ctcgaggact tcctgcgctc gaccttctgc acggtgcgcg aacgctccac
     2101 cgccgcgcgg atcgcaagct cggcgatttt cctgccgtag tcgacgtggg tgttcagcca
     2161 cagttcgaag ggatcgcgca ccatcaccga aacgagtttc agtgcgtcgc gattggaaag
     2221 cttctccttg gtctgaccgt ggaactgcgg ttccagcacc ttggcggaga ggaagtacac
     2281 ggtgcgcgac cacgcatcgt ccgcctgcag gcgtatgccg cgcggcatca tgttgtggtg
     2341 ctcggcaaag gcgcggatcg cgtcgaaaat gccgtcacgc agccccgctt cgtgcgtgcc
     2401 gcccgccggc gtagggatga ggttcgcgta cgattcgccg tagcccttgc ccgcctccac
     2461 gaaggcgaat gcccagcccg caccctcacc cgccgcgaag ccgttcgcct cctgcacgta
     2521 cttctcgccg gcgaagatgg gcgccaccgg ctctgcttca gccagcagct cttcgagata
     2581 gcctttcatg ccttcgggat agctccaggt gcggctcgtc gtttccttcg cgccttcgat
     2641 gttgagggtg actttgacgc ccggcagcag cacggccttg gagcgcacga tgcgttcgag
     2701 atcgggaagc gagactttcg gagtatcgaa atacttcgca ttgggccaga tgcgcagcgc
     2761 cgtaccggta tcgttgcgcg ggcacttgcc cttcaccttg agcttttgcg cgaccgcccc
     2821 attggcgaag cccatcgcgt gaatctggcc atcgcgccgc acttccactt cgaggcgttc
     2881 ggaaagcgca ttggtgaccg aaacaccgac accgtgcagc ccgccggaaa acttgtatgc
     2941 g
//
LOCUS       DASURG010000169         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_235697, whole genome shotgun sequence.
ACCESSION   DASURG010000169 DASURG010000000
VERSION     DASURG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235697"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFY80_02810"
     CDS             <1..478
                     /locus_tag="VFY80_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6006864.1"
                     /translation="DGTADWLWPAAERAGLPVGLLCPNRINVVGEIAAKHPKLRLLID
                     HMARPRHIKDDASFADLGDLLALARYPNVAVKATGAPSYSSDPWPYRNIHKYLKQIFD
                     AFGPSRMFWGTDITRMPCTWKQCVTMFTEELPWLKGRDLELVMGRALCDWIDWRVD"
     gene            complement(561..1550)
                     /locus_tag="VFY80_02815"
     CDS             complement(561..1550)
                     /locus_tag="VFY80_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006865.1"
                     /translation="MTNRNRRSCITRVITVLGFGIAASVAQAADSTYPTRPVRLVVPF
                     APGGGVDATARIVAPKLSESMGQNWVVDNRTGAAGNIASEIVARANPDGHTVLLALDT
                     QLSANPSLYKLPFNVTKDLQPVVILAVSDQIVVVHPGVPAKTLKEFIALAKQKPGALR
                     HGSAGVGSSNHLAAELFKKVTGLNMVHVPYKGAAPAAAAILAGEIQMNISSTASTIGH
                     IQAGRLRALASTGLKRGKALPDLPTVAESGYPGFEAIQWYGLVVPGATPKSIVARIHA
                     DSVKALQSADVRAAMDRLGLGQEPSTPEALRARIKKETATWAAIIKDAGIRVQ"
     gene            complement(1589..>2413)
                     /locus_tag="VFY80_02820"
     CDS             complement(1589..>2413)
                     /locus_tag="VFY80_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6006866.1"
                     /translation="APTQPGVGAVVEMACVDLACRLLDVPMYSYLGGAVHDRVEFNGW
                     IGMLPPDEAAAEAKRWLDAGFRSAKIKVGSGVEADRDRIAAVRDTVGSAMKLRIDANC
                     QYDAETSLKLCRVIKPFDLQLFEQPVPKDDIAGMARVRREGGVPVMADETVDSHESLL
                     AVIKADAADFVKFGIKQAGGILRAARMLATAQAAGVPVVMGHGFGLDPSTLAEIMLAA
                     SSRAVLSGLECVGPLKVVDTVATTRLDISSGSLALPPGPGIGITLDDAKLEKYRLH"
BASE COUNT          384 a          785 c          809 g          435 t
ORIGIN      
        1 cgacggcacc gcggactggc tgtggccagc agccgagcgc gcggggctgc cggtgggtct
       61 gctatgcccg aaccggataa acgtcgtcgg cgagatcgcc gcgaagcatc cgaagttgcg
      121 actgctgatc gaccacatgg cgcggccgcg ccacatcaag gacgacgcga gcttcgctga
      181 tctcggcgat ctgctcgcgc tcgcgcgtta tcccaatgtg gcggtcaaag cgacgggcgc
      241 accgagctac tcgagcgatc cgtggccgta ccgcaacatt cacaaatatc tgaagcagat
      301 cttcgacgct ttcggacctt cgcgcatgtt ctggggcacg gacatcaccc gcatgccgtg
      361 cacgtggaag cagtgcgtca cgatgtttac cgaggagttg ccgtggctca aggggcgcga
      421 tctggagctg gtgatggggc gggcgttgtg cgattggatc gactggcgcg tggattgaat
      481 gacgtgctca aggactggcg gcgttcgccg cagagtcctt gcgctttctg cgcgacgcga
      541 taccggatgc ggtctgcagc ttactgaacg cggattcccg cgtctttgat gatggcggcc
      601 cacgtggccg tctctttttt gatgcgcgcg cgaagcgcct ccggcgtgct tggttcctgc
      661 cccagaccca ggcggtccat ggccgcccgc acgtcggcac tttgcagcgc cttcaccgag
      721 tccgcatgaa tgcgcgcgac gatgctcttc ggcgtcgcgc ccggcacgac cagtccgtac
      781 cactggatcg cctcgaatcc ggggtagccg gactccgcta cggtcggcag gtccggcagc
      841 gccttgccgc gcttcaggcc ggtactggcc agcgcgcgca gccgacccgc ctggatgtgc
      901 ccgatcgtgg aggcggtgga gctgatgttc atctggatct cgcccgcgag tatggcagcg
      961 gccgcgggag ccgcgccttt gtacggcacg tgtaccatat tgagcccggt aaccttcttg
     1021 aagagttcgg cggcgaggtg attcgagctc cccacgccgg ccgatccatg gcgcagcgca
     1081 ccgggcttct gcttggccag cgcgatgaac tccttcagcg tcttggcggg aaccccggga
     1141 tggacgacca cgatctggtc gctcacggcc agtatgacga cgggctggag gtctttcgtg
     1201 acgttgaacg gcaatttgta aaggctcgga ttcgcgctga gctgggtgtc gagtgccaac
     1261 aacaccgtgt ggccgtccgg gtttgcgcgc gccacgatct cgctcgcgat gttgcctgct
     1321 gcgccggtgc gattgtccac gacccagttc tggcccatgg actcgctcag cttcggagcg
     1381 acgatgcgag cggtcgcatc gacaccgccg ccgggcgcga acgggacgac cagccggaca
     1441 gggcgggtcg gatatgtgga atcggcggcc tgcgcaacgc tcgcggcgat gccaaagccg
     1501 agcacggtga tgacgcgcgt gatgcaactc cggcggttgc ggttcgtcat ggctatcctc
     1561 ctcgttattg gaatgttgga ccgcctcatc agtgcaggcg atatttctcc agcttggcgt
     1621 cgtcgagcgt gatgccgatc cccggtcccg gcggcagcgc gagactgccg gaggagatat
     1681 cgagccgcgt cgtcgcgacg gtatcgacga ctttcagcgg ccccacgcac tcgaggccgg
     1741 agagcacggc gcgcgaggaa gccgcgagca tgatctcggc aagcgtgctc gggtcgaggc
     1801 cgaagccgtg gcccatcacg accggcacac ccgccgcttg cgccgtggcg agcatgcgcg
     1861 ccgcgcgcag gatgccgccc gcttgcttga tgccgaactt cacgaaatcc gccgcatccg
     1921 ccttgatcac agcgagcagg ctctcgtggc tgtcgacggt ctcatccgcc atgaccggca
     1981 cgccaccttc gcggcgaacg cgcgccatgc cggcgatgtc gtccttgggc accggctgct
     2041 cgaagagttg caggtcgaag ggcttgatga cgcggcagag cttgagcgag gtctccgcgt
     2101 cgtactggca gttcgcatcg atccgcagtt tcatcgcgct tcccaccgtg tcgcgcacgg
     2161 ccgcgatgcg gtcgcgatcg gcctccacgc cgctgccgac cttgattttc gcggagcgga
     2221 aaccggcatc gagccagcgc ttcgcttccg ccgcggcttc gtccggaggg agcatgccga
     2281 tccaaccgtt gaattcgacg cggtcgtgca cggcgccgcc aaggtagctg tacatcggca
     2341 cgtcgagaag acggcaggcg agatccacgc acgccatttc gacgaccgcg cccacgccgg
     2401 gctgcgtcgg ggc
//
LOCUS       DASURG010000170         5804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_745617, whole genome shotgun sequence.
ACCESSION   DASURG010000170 DASURG010000000
VERSION     DASURG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745617"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /gene="pnp"
                     /locus_tag="VFY80_02825"
     CDS             <1..1735
                     /gene="pnp"
                     /locus_tag="VFY80_02825"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6006867.1"
                     /translation="DIPAMIGASAALAISGIPFNGPIGAARVGYLDGQYVLNPTATEL
                     TTSQLDLVVAGTQQAVLMVESEAKELPEDVMLGAVVYGHQQMQSVIEAIHDLVDEAGR
                     ALWDWSAPQKDEALVARIAELAERDLNAAFELKQKQARSHAIDDIWTRVFNELGVGTE
                     NGPPANIVKEICFALESKIVRGQILNGEPRIDGRDTRTVRPISIRTGVLPRAHGSALF
                     TRGETQALVTATLGTSRDEQIIDALQGEYRERFMLHYNMPPYATGETGRVGSPKRREI
                     GHGRLAKRALLTVLPTPEEFAYSLRVVSEITESNGSSSMASVCGGCLSLMDAGVPLKA
                     HVAGIAMGLIKEGNRFAVLSDILGDEDHLGDMDFKVAGTARGITALQMDIKIEGITKE
                     IMHAALTQAREGRMHILEKMRGAIESPRSEISAYAPRMITMKIKPEKIRDVIGKGGAV
                     IRALTEETGTTIDIEDDGTVTIACVSSEGGEIARRRIEELTADVEVGRIYEGTVLKLL
                     DFGAIVSVLPGRDGLLHISQIANERVNSVGDYLKEGQTVRVKVLEADDKGRLRLSMKA
                     AAAEAQAATAQ"
     gene            complement(1810..2232)
                     /gene="dksA"
                     /locus_tag="VFY80_02830"
     CDS             complement(1810..2232)
                     /gene="dksA"
                     /locus_tag="VFY80_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX6006868.1"
                     /translation="MPTELHKAFPPYKPKGKEEYMNARQLTHFRKMLEEMKRELSQDI
                     DRTVHTMQDEATIFADPNDRASQESDMSLELRNRDRERKLIKKIDETIARIDAGEYGY
                     CESCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            2459..2535
                     /locus_tag="VFY80_02835"
     tRNA            2459..2535
                     /locus_tag="VFY80_02835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2493..2495,aa:Arg,seq:tct)
     gene            2558..2633
                     /locus_tag="VFY80_02840"
     tRNA            2558..2633
                     /locus_tag="VFY80_02840"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2591..2593,aa:His,seq:gtg)
     gene            complement(2712..3680)
                     /locus_tag="VFY80_02845"
     CDS             complement(2712..3680)
                     /locus_tag="VFY80_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006869.1"
                     /translation="MTSRARCIISLVLTLTASFACFAAAPPWPTKPIRLVVNFPPGGT
                     TDQMARALGPRLAETFGQPVVIDNRGGAGGNIGLEVVAKSAPNGYTLLISSGSPITVG
                     PHLYRLGFDVSKALAPVAPTARTPILLVVQPGLPIRTFAELLAHARAHPGKLTFGSAG
                     NGTTLHIHGERLLRTAKIQATHVPYKGVAPMIVALLGGQIDFAFDSGLTIQHVKAEKL
                     RLLAVLSPARSPLFPDTPTLAEMGTVVDPEPLFGVYAPAGTPHEIIMRLNREITRIMQ
                     TAEVRAALATFGSEVVTASPEEFAAIQRRDRERQGAFIREANIRLD"
     gene            3850..4293
                     /locus_tag="VFY80_02850"
     CDS             3850..4293
                     /locus_tag="VFY80_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6006870.1"
                     /translation="MQTLWWHWIVLGIVLMLLELAVPSFFLVWFGLGALIIGLTLVFL
                     PAAPFAFQILGWTASSLLFVVLWFKVFKPNIFKSRAGMARGSLIGEVGLVTRDIRPYE
                     KGQVRFQKPILGDEVWESVSDQEIRVGERVRVLDVEGNTLKVSRA"
     gene            4305..5165
                     /locus_tag="VFY80_02855"
     CDS             4305..5165
                     /locus_tag="VFY80_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX6006871.1"
                     /translation="MGGFEIFAIAVLIFVVVTAWKGVRIVPQGEEWVVERFGKYERTL
                     TPGLSIIIPYFDKVAYKVVTKDIILDVPEQDVITRDNAVIITNAIAFIKVTDPVKAVY
                     GVTDFAEAIRNLVQTTLRSIVGEMELDAALSSREKIKARLREMISDEAIDWGLTIKSV
                     EIQDVKPSESMVKSMELQAAAERERKAVVTKAEGEKQAMILEAEGRLESAKRDAIAQV
                     TLAESSSAAIQKVAEAVGDRDTPMLYLLGEKYILALDRLATSGNSKIVLLPADLQDTL
                     RGIFSKGRAN"
     gene            complement(5167..5610)
                     /gene="smpB"
                     /locus_tag="VFY80_02860"
     CDS             complement(5167..5610)
                     /gene="smpB"
                     /locus_tag="VFY80_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX6006872.1"
                     /translation="MSIVQNRKAFHDYFIEERFECGIALEGWEVKAIRAGRVQLKEAY
                     VIVSHGEIFLVGCHVSPLTTASTHVTPDPTRTRKLLLHAAEINKLIGRVERAGYTLVP
                     LDMHYSHARIKLEIGLAKGKKQHDKRAAEKEKAWRREQQRLLKSA"
     gene            5704..>5804
                     /locus_tag="VFY80_02865"
     CDS             5704..>5804
                     /locus_tag="VFY80_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-binding protein"
                     /protein_id="HEX6006873.1"
                     /translation="MPEVTKSVLVVHTPEEMFALVDAVERYPEFLPW"
BASE COUNT         1140 a         1688 c         1851 g         1125 t
ORIGIN      
        1 cgacataccg gcaatgatcg gcgcgtccgc ggcgctcgcc atttccggca ttccgttcaa
       61 cggaccgatc ggtgcagcgc gggtcggcta tctcgatggc cagtacgtgc tcaaccccac
      121 cgcgacggaa ctgaccacct cccagctcga tctcgtcgtc gccggcacgc agcaggcggt
      181 gctgatggtc gaatccgaag ctaaagagct tcctgaagat gtgatgctcg gcgcggtagt
      241 ctacgggcac cagcagatgc agtccgtgat cgaggccatc cacgatctcg tggacgaggc
      301 cggccgggcg ctgtgggact ggagcgcgcc gcagaaggac gaggcgctcg tcgcgcgcat
      361 cgccgagctt gcggagcgcg accttaacgc agcgttcgag ctgaagcaga agcaggcccg
      421 ttcgcacgcg atcgacgaca tctggacgcg cgtctttaac gagctgggcg tcggaaccga
      481 gaacggtccg cccgccaaca tcgtcaagga aatctgcttc gcgctggagt caaagatcgt
      541 gcgcggccag attctgaatg gcgagccgcg tatcgatgga cgcgacaccc ggacggtgcg
      601 gccgatatcg atccgcaccg gcgtgctgcc gcgcgcgcac ggatcggcgc tttttacgcg
      661 cggtgagacg caggcattgg tcaccgcaac gctcggcacc tcgcgcgacg agcagatcat
      721 cgacgcgctg cagggcgaat atcgcgagcg tttcatgctc cactacaaca tgccgcctta
      781 tgcgactgga gaaaccggcc gggtgggatc tcccaagcgg cgcgagatcg gtcatggccg
      841 cctcgccaag cgcgcactgc tcaccgtgct cccgacaccc gaggaatttg catattcact
      901 ccgtgtggtg tccgagatca ccgaatcgaa cggctcgagc tcgatggcct cggtgtgcgg
      961 aggctgcctt tcgctgatgg atgccggcgt tccgctcaag gcgcacgttg ccggcattgc
     1021 gatgggcctc atcaaggaag ggaaccgctt tgcggtgctg tccgacatcc tgggtgacga
     1081 ggatcatctc ggagacatgg atttcaaggt ggcgggtact gcgcgcggca ttacggcgct
     1141 gcagatggac atcaagatcg agggcatcac caaggagatc atgcacgccg cgctgacgca
     1201 agcccgcgaa gggcggatgc acatcctgga aaagatgaga ggcgccatcg aaagcccgcg
     1261 ctccgaaatc tcggcgtatg cgccccgcat gatcaccatg aagatcaagc ccgagaagat
     1321 ccgcgacgtg atcggcaagg gaggcgccgt aatccgcgcg ctgaccgagg agaccggcac
     1381 gacgatcgac atcgaagacg acggcaccgt caccatcgca tgcgtgtcct cggaaggcgg
     1441 agaaattgcg cggaggcgca tcgaggagtt gactgcagac gtggaagtcg ggcgcattta
     1501 cgagggcacc gtgctcaagc tgctcgattt cggcgcgatc gtgagcgtgc tgcctggccg
     1561 cgacgggctg ctgcacattt cccagattgc gaacgagcgc gtgaacagcg tcggcgacta
     1621 tctcaaggaa ggtcagacag tgcgggtaaa agtactggaa gcggatgaca aggggcgcct
     1681 gcggctgtcc atgaaggcgg ctgctgccga agcacaagcg gcaaccgcac agtagccgga
     1741 ctgcaggaaa tcaaaaaggg cggtttctca agccgccctt tttatttact ctatcgtcgg
     1801 acgcagccgt tacttcgcga cctgccgctc ccgcagttcg tccagcgtct tgcagtcgat
     1861 gcagagtgtc gccgtcgggc gcgcctcgag ccgcttcagg ccgatttcca cgccgcagga
     1921 ttcgcagtat ccatactccc ctgcatcgat gcgcgcgatc gtctcgtcga ttttcttgat
     1981 gagcttgcgc tcgcggtcac ggtttcgcag ctcgagtgac atgtccgact cctggctcgc
     2041 ccgatcgttg ggatcagcaa agatcgtcgc ctcgtcctgc atcgtgtgaa cggtccggtc
     2101 gatatcctgc gagagctcgc gcttcatctc ttcgagcatc ttgcgaaaat gcgtgagctg
     2161 ccgggcgttc atgtattcct ctttcccctt gggcttgtag ggcgggaaag ccttgtgcag
     2221 ttcggtcggc atgtcagtct gtccgtaaaa aaagcgcttt aatatcagaa attcacagcc
     2281 accgcaacca acgagcggca cactctcttc cgccggcagc ttcgttgacc gtgcggggtg
     2341 cgcagagtat attacgcgcc ttcggttagg gcgtgccggc ggacgagatt aaacgcgatc
     2401 cgggcgcgtg agcgttcttt ttagcacggc cgccgcatta caatgacgtc tgctcgaggt
     2461 gcccgtagct caaccggata gagcaccggc cttctaagcc gggggttgtg ggttcgagtc
     2521 ccgccgggcg cgccacaacc actttcgcgt ttcgatggtg gcagtagctc agctggtaga
     2581 gtcccggatt gtgattccgg ttgtcgtggg ttcgagtccc atctgccacc ccaacttccg
     2641 ctcggtcgag gacagccgat cgccacatcg gcgatcgcca ggatcgacgc ccgatctcct
     2701 tgccggccga atcaatccag acggatgttc gcctcgcgta taaacgcgcc ttgacgttcg
     2761 cggtcgcgcc gctggatcgc cgcgaattct tcgggagatg cggtgacgac ttcggagccg
     2821 aaggtggcga gcgctgcacg tacttcggca gtttgcatga tacgagtgat ttcacggttc
     2881 aagcgcatga tgatttcgtg cggcgtgccg gcgggcgcat agacgccgaa cagcggctcg
     2941 ggatccacca ccgtacccat ctcggccaaa gtcggcgtgt cggggaatag gggcgagcgc
     3001 gcggggctca acaccgcgag cagccgaagt ttttcggcct tgacgtgctg aatcgtgaga
     3061 cccgaatcaa acgcaaaatc gatctgcccg ccgagcaggg ccacaatcat gggcgccacg
     3121 cctttgtagg gcacgtgggt ggcttggatc tttgcggtgc gcaacagcct ctcgccgtgt
     3181 atatgcagcg tcgtgccgtt ccccgccgag ccgaaagtca acttccccgg atgggctcgc
     3241 gcgtgggcga gcaactctgc aaaggtgcgt atcggcaggc cgggctgcac gacgagaagt
     3301 atcggagtac gcgccgtcgg cgcaacgggg gccagcgcct tcgatacgtc gaagcccagc
     3361 ctgtacagat gcggcccgac ggtaatagga ctgccggacg agatgagcag cgtgtagccg
     3421 tttggcgccg acttggctac gacttccagc cctatatttc caccggcccc gccacggttg
     3481 tcgatcacga caggctggcc gaacgtctcg gcgagccgcg gtcccagcgc gcgagccatc
     3541 tgatcggttg tgccgcccgg tggaaaattg acgacgagcc ggatgggctt ggtaggccac
     3601 ggcggcgcgg cagcgaagca cgcgaatgac gcagtcagcg tgagtacaag cgaaattatg
     3661 caacgtgcgc gcgaagtcat gccccctcct gaaaagctgc tggcccgccg aatgccaagt
     3721 gctatcggct taacacagct gcaacttgat acagatcaga tttttgcctt gagcgcggct
     3781 ctcgtggtct aattgccggc tcttgcgtgc ggtgcgctag acactgccgc gggtcacagg
     3841 gggatggaaa tgcagacgct ctggtggcac tggatcgtgc tcggcatcgt attgatgctg
     3901 ctggaactcg cggtgccttc atttttcctg gtctggttcg gcctcggcgc actgatcatc
     3961 ggtctcacgc tggtattctt gccggcagct ccatttgcat tccagatact gggctggacc
     4021 gcatcgtcgc tcctttttgt cgtcctgtgg ttcaaggtct tcaagccgaa catcttcaaa
     4081 tcccgggcgg ggatggcacg cggctccctg atcggcgaag tgggactggt cacgcgtgac
     4141 attcggccct atgagaaggg ccaagtgcgg ttccagaagc cgatactggg cgacgaggta
     4201 tgggagtcgg tgtcagacca ggaaatacgg gtgggcgagc gggtgcgggt gctggatgta
     4261 gaaggcaaca cgttgaaggt cagcagagcg taaaggggag agcgatgggc ggcttcgaga
     4321 tattcgcgat tgcggttttg atattcgtcg tagtcacggc ctggaagggc gtgagaatcg
     4381 ttccccaagg tgaggaatgg gtggtcgagc gcttcggcaa gtacgagcgc acgctgacgc
     4441 cggggctcag catcatcatc ccgtacttcg acaaagtcgc atacaaggtg gtcacaaaag
     4501 acatcattct ggatgttccc gaacaggatg tgataacgcg ggacaacgcc gtcattatta
     4561 cgaacgccat cgccttcatc aaggtcaccg acccggtaaa ggcagtgtac ggcgtgactg
     4621 attttgctga ggcgatccgc aatctggtgc agactacctt gcgttccatc gtcggcgaaa
     4681 tggaactgga tgcggcactg tcttcgcgcg aaaagatcaa ggcacggttg agggaaatga
     4741 tctcagacga agcgatcgac tggggactca ccatcaagtc ggtcgaaatc caggatgtga
     4801 agccctccga atcgatggtg aaatcgatgg agcttcaagc cgcggccgag cgggaaagaa
     4861 aggcagtcgt caccaaggca gaaggcgaaa agcaggcaat gatactggag gcggaaggac
     4921 ggctcgagag cgcgaagcgc gatgcgatcg cccaggtgac gctcgcggaa tcttcatccg
     4981 ccgccattca gaaggtcgcc gaggccgtag gcgatcgcga tacgccgatg ctctatctgc
     5041 tcggtgaaaa atacattctg gcactcgacc ggctcgccac gtcgggaaac agcaagatcg
     5101 tcttgttgcc ggccgacctg caggatacgc tgcgcggtat cttcagcaag ggccgagcca
     5161 actgattcag gcgctcttca gcaacctctg ctgctcgcga cgccaggctt tctctttctc
     5221 cgcagcccgc ttgtcatgct gcttcttgcc cttcgcgagc ccgatttcga gcttgatgcg
     5281 cgcgtggctg taatgcatat cgagcgggac gagtgtatag cctgcgcgct cgacgcgacc
     5341 aatgagcttg ttgatctcgg ctgcgtgcag cagcagcttt cgggtgcgag tgggatcggg
     5401 cgtgacatga gtcgatgccg tggttagcgg gctcacgtga cagcccacga ggaagatctc
     5461 gccgtggctt acgatcacgt acgcttcctt cagctggacg cgacctgcgc ggatcgcctt
     5521 tacctcccaa ccctcgagcg cgatgccgca ttcgaagcgt tcctcgatga agtaatcgtg
     5581 aaacgctttt cggttctgta cgatgctcat cgcgggcgcc gcggggaagg gaatgctatt
     5641 ctagcagtcc gcttgctcac ggactggcgt gacgaggggc ctgacaggag cgaagagaga
     5701 gccatgcctg aggtcacgaa gtccgtgctg gtggtgcata caccggagga gatgtttgcg
     5761 ctggtcgatg cggtggagcg ctacccggaa ttcctgccgt ggtg
//
LOCUS       DASURG010000171         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_439549, whole genome shotgun sequence.
ACCESSION   DASURG010000171 DASURG010000000
VERSION     DASURG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439549"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /locus_tag="VFY80_02870"
     CDS             <1..2006
                     /locus_tag="VFY80_02870"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX6006874.1"
                     /translation="TTIHCDHLIQARKDGLSDLRASLAENTEVYDFLRSAAARYGAGF
                     WGPGAGIIHQVVLERYAFPGQLIIGTDSHTPNAGGLGACAVGVGGADAVETIAGLPWE
                     LLYPRRIAVYLTGKLGGWTAPKDVILYVAGALTVSGGTNAIVEYIGPGARTLSATGKA
                     TITNMGAELGATTSMFPADERMAIYLHATGRSNLVPPMEQYRALLEPDAEVETNPERH
                     YDRVLRIDLSTLEPHVVGPHTPDRARPISRLAAEVSDAGNGFVDRISAALIGSCTNSS
                     YEDMSRAADVAQQAHARGLEASAAFMVTPGSEQVRATMERDGQMQALERANATVLANA
                     CGPCIGQWRRAKDAAAAPNTIVTSYNRNFPARNDGQAATMNFIASPEIVTALAIAGRL
                     SFNPLTDTLTGADGKPFRFEPPRPAPEVPPRNFEHGKVDYVPPPANGSSVEIVVDPRS
                     ERIQLMQPWPAWDGVDFVDMPVLLKTKGKTTTDHISPAGPWLRYRGHLDRFSDNMFLG
                     AINAYTGVAGQSKNALTGETHSVADNARDYRGRGIRWVVIGDANYGEGSSREHAALSP
                     RLLGGVAVIARSFARIHESNLKKQGLLALTFQNPAHYERIREDDRLSLLGLSAMAPGK
                     PVECRVRHADGEVETLWLDHTFSASQTKWFRAGSALNAAYENG"
     gene            complement(2043..2471)
                     /locus_tag="VFY80_02875"
     CDS             complement(2043..2471)
                     /locus_tag="VFY80_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6006875.1"
                     /translation="MKRIRIAALVMGALLISAAQDAYAQQQKKKTDFGKREYESKCAV
                     CHGVAGKGDGPYKPWLTTSPSDLTVLAKNNGGVFPFDRVYNIIDGRLTVRAHGPADMP
                     IWGADYKAESAYFDVPYDPEAYVRSRILALTEYVSRLQAK"
BASE COUNT          476 a          818 c          817 g          437 t
ORIGIN      
        1 cgacgaccat ccattgcgat cacttgatcc aggcacgcaa ggacggtctc agcgatctgc
       61 gcgcttcgct cgcggagaac acggaggtct acgacttcct gcgttctgcg gccgcccgat
      121 acggcgccgg tttctgggga cccggcgcgg gcatcatcca ccaggtcgtg ctcgagcgtt
      181 acgcattccc gggccaactt atcatcggca ccgactcgca tacgccgaac gcgggcgggc
      241 tcggcgcctg cgcggtgggt gtcggcgggg cggacgccgt tgagaccatc gcaggcctgc
      301 cgtgggaact cctgtatccc aggcgcattg ccgtgtacct gaccgggaaa ctgggcggct
      361 ggaccgcgcc gaaggatgtg atcctctacg tcgcgggcgc gctgacggtc tcaggcggca
      421 ccaatgccat cgtcgagtac atcggtcccg gcgcgcgcac cctcagcgcg accggtaaag
      481 cgacgatcac caatatgggc gccgaactgg gcgcgacaac ctccatgttc cccgcggacg
      541 agcgcatggc gatctatctg catgcgacgg gacgcagcaa tctcgtgccg ccgatggagc
      601 agtatcgcgc gttgctcgag ccggacgcgg aagtggagac caatcccgag cggcactacg
      661 atcgcgtgtt gcgtatcgac ctttcgacgc tcgagccgca tgtggttgga ccgcataccc
      721 cggaccgtgc gcgcccgata tcgcggctgg cggcagaggt gagcgatgcc gggaacgggt
      781 ttgtcgaccg tatttcggcc gcgttgatcg gcagctgcac caactcgtcg tatgaggata
      841 tgagccgcgc cgccgacgtc gcgcaacagg cccatgcgcg gggtctcgag gcgagcgctg
      901 cgttcatggt tacgccaggg tcggagcagg tgcgcgccac catggaacgc gacggccaga
      961 tgcaagcgct cgagcgagcc aacgccactg tccttgccaa tgcctgcgga ccctgcatcg
     1021 ggcaatggcg cagggcgaag gacgctgccg cggcgccgaa caccatcgtc acgtcctaca
     1081 accgcaattt tccggcgcgc aacgacggcc aggcggccac gatgaatttc atcgcgagcc
     1141 cggaaatcgt caccgcgctc gccatcgcgg gcaggctctc gttcaacccg ctgacggata
     1201 ccctcaccgg cgccgacggc aagccgttca ggttcgagcc gccacgcccc gcgcccgaag
     1261 tgccgccgcg caacttcgag cacgggaagg tggactacgt cccgccgcct gcgaacggca
     1321 gcagcgtgga gatcgtggtc gacccccgca gtgaacgaat ccagctgatg caaccgtggc
     1381 ccgcgtggga tggcgtcgat ttcgtggata tgccggtcct gttgaagacg aaaggcaaga
     1441 ccacgaccga ccatatctcc ccggccgggc cgtggctgcg gtatcgcggt catctcgacc
     1501 ggttcagcga caacatgttc ctcggcgcga tcaatgcgta taccggagtg gcggggcaga
     1561 gcaaaaacgc gctgaccgga gaaacccact ccgttgccga caacgcgcgt gactaccgag
     1621 ggcgcggtat cagatgggtc gtgatcggag acgccaacta tggcgagggc agcagccgtg
     1681 agcacgcggc gctctcgccg cgcctgctcg gcggtgtggc tgtcatcgcc cgcagctttg
     1741 cgcgcataca cgagtccaat ctgaaaaagc agggcctgct cgcgctcacc tttcaaaacc
     1801 cggcgcatta cgagcgcata cgggaagacg atcgtctcag tctgctgggg ctttctgcca
     1861 tggcgcccgg gaagccggtg gagtgccggg tgcgccatgc ggacggtgag gtcgagacgc
     1921 tatggctcga ccacaccttc agtgcatccc agaccaaatg gtttcgggcc gggtcggcgc
     1981 tcaatgcggc ttacgagaac ggataacgcg gtatttcagg cgcgggcaga atcgaaggat
     2041 cactacttcg cctgcagccg ggagacatac tccgtcagcg ccaggatccg gctgcgcaca
     2101 tacgcctccg gatcgtaggg cacatcgaaa taggccgatt cggccttgta gtccgccccc
     2161 cagatcggca tgtctgcagg gccgtgagcg cgcacagtga gccgcccgtc gataatgtta
     2221 tacacgcggt cgaagggaaa tacgccgccg ttattcttcg caaggacggt cagatcagac
     2281 ggactcgtgg tcaaccacgg cttgtagggt ccatcaccct tgcctgcaac gccgtggcag
     2341 acagcgcatt tcgactcgta ctcccgcttg ccgaagtcgg tcttcttctt ctgttgttgg
     2401 gcgtatgcgt cttgcgccgc ggagatcagc aatgccccca tcaccaaggc tgcgattctg
     2461 atccgtttca cggaaactcc tgcggcgtgg ctgtattgag aacgccgatg aaagcgctga
     2521 gcgaatacga tgtatgcgat tcgggatt
//
LOCUS       DASURG010000172         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_541857, whole genome shotgun sequence.
ACCESSION   DASURG010000172 DASURG010000000
VERSION     DASURG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541857"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /gene="lolA"
                     /locus_tag="VFY80_02880"
     CDS             <1..529
                     /gene="lolA"
                     /locus_tag="VFY80_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX6006876.1"
                     /translation="LNGTQAASARFAQMVLDSNMKMLQQASGTMEFSRPGKFRWVYHK
                     PYEQVIVSDGSRLWIYDKDLNQVTVRSLDRALGSSPAALLAGKDVLEENYTLNNIGTQ
                     EGLEWVEAVPKRQDTAFERVRIGFGKTGLEAMELRDQFGQITVIKFADVERNPKLPAG
                     TFEFKPPPGADVIRE"
     gene            522..1841
                     /locus_tag="VFY80_02885"
     CDS             522..1841
                     /locus_tag="VFY80_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6006877.1"
                     /translation="MNDLFAKRAPQPPLAEALRPRTLDEVVGQSHLVGPGKPLRLAFE
                     AKKPHSMILWGPPGVGKTTLARLMAEAFNAEFIAISAVFSGVKDIREAVQRAELALQQ
                     HGRSTILFVDEVHRFNKAQQDAFLPYVEQGLITFVGATTENPSFEVNSALLSRAAVYV
                     LQPLTDAELGLLFDRARVHALKGVEVAPQARDLVIAYADGDARRLLNMVEQIGTAASE
                     AGTTTIDIEFARSTLTQNLRRFDKGGDQFYDQISALHKSVRGSSPDAALYWLVRMLDG
                     GADPLYIARRIVRMATEDIGLADPRGLRVALDACETYERLGTPEGELALAQAVIYLAV
                     APKSNAVYAAYNSARDFVGKDRSRPVPEHLRNAPTRLMKDLGYGREYRYAHDEPDAYA
                     AGERYFPEGLDAQSFYAPTSQGLEARIRERLEHLRELDRKASKTRQS"
     gene            1926..>2261
                     /locus_tag="VFY80_02890"
     CDS             1926..>2261
                     /locus_tag="VFY80_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6006878.1"
                     /translation="MLDIQLLRGDLAATAQRLASRGFQLDTTAFQALEAERKSVQVET
                     QALQSRRNALSKQIGQLKAKGEDASVLMAQVNAQAEELKTLEQRLSDVQQKLNDFLLV
                     VPNLPHASVP"
BASE COUNT          415 a          745 c          752 g          349 t
ORIGIN      
        1 tctcaacggg acacaggccg cgagcgcgcg cttcgcccag atggtgctcg acagcaacat
       61 gaagatgctg cagcaggcaa gcggcaccat ggagttctcg cggccgggca agttccgctg
      121 ggtgtatcac aagccgtacg agcaggtcat cgtgagcgac gggtcgcgac tctggatcta
      181 cgacaaggat ctgaaccagg tgaccgttcg gtcgctggac cgggcgctgg gcagcagtcc
      241 cgcggcgctc ctcgcaggga aggacgtgct cgaggagaat tacacgctca acaatatcgg
      301 cacgcaggaa ggcctcgaat gggtagaagc ggtacccaag cggcaggaca ccgcattcga
      361 gcgggtgcgc atcggcttcg gcaagaccgg tctcgaagcg atggagctgc gcgaccagtt
      421 cggccagatc acggtgatca agttcgccga cgtggagcgc aatccgaagc tgcccgcggg
      481 cacgttcgag ttcaagccgc ccccgggtgc ggacgtcatc cgtgaatgac ctcttcgcaa
      541 agcgcgcgcc gcaaccgccg ctcgccgaag cgctgcggcc gcggacgctc gatgaagtcg
      601 tcggccagtc gcatctcgta ggaccgggca agcccctgcg gctcgcgttc gaagcgaaaa
      661 agccgcactc gatgatcctg tggggcccgc cgggcgtggg caagaccacg ctggcgcggc
      721 tcatggccga agcgttcaac gccgaattca tcgcgatctc cgcggtattc tccggagtca
      781 aggacatccg cgaagccgtg cagcgcgccg agcttgcgct ccagcagcac ggccggtcca
      841 ccattctgtt cgtcgacgaa gtgcaccgct tcaacaaggc ccagcaggat gcctttctgc
      901 cgtacgtgga gcaggggctc atcacgttcg tcggcgccac cacggaaaat ccatccttcg
      961 aggtgaacag cgcgctgctc tcgcgcgcgg cggtctatgt gctgcagccg ctcaccgacg
     1021 ctgagctcgg cctgctcttc gaccgggccc gggtgcacgc gctgaaaggc gtcgaagtcg
     1081 ccccgcaggc ccgcgacctc gtgatcgcgt acgccgacgg cgatgcgcgg cgtctgctca
     1141 acatggtgga gcagatcggc acggccgcat ccgaggcggg caccacgacg atagatattg
     1201 aatttgcgcg cagcacgctc acgcagaacc tgcggcgctt cgacaagggc ggcgaccagt
     1261 tctacgacca gatatcggcg ttgcacaagt ccgtgcgcgg ctcttcgccc gacgcggcgc
     1321 tctactggct cgtgcgcatg ctggatggcg gcgccgaccc cctgtatatc gcccggcgca
     1381 tcgtgcgcat ggccaccgag gacatcgggc tcgcggatcc gcgcgggctg cgggttgcgc
     1441 tcgacgcctg cgagacctac gagcggctcg gcaccccgga aggcgagctc gctctggcgc
     1501 aggcggtgat ctatctcgcg gtggcgccga agagcaacgc cgtttacgcg gcatacaaca
     1561 gcgcgcgcga tttcgtcggc aaggaccgct cgcggcccgt gcccgagcat ttgcgcaatg
     1621 cgccgacccg gctcatgaag gacctgggtt acggccgcga gtatcgctac gcgcacgacg
     1681 aaccggacgc gtacgccgcg ggcgagcgct actttcccga gggcctggac gcgcagagct
     1741 tctacgcgcc gacgtcgcag gggctggagg cgcggatccg ggaacggctc gagcacctgc
     1801 gcgaactcga tcgcaaggcc tccaagacgc ggcaatcgta ggcggcgcct acgcccggac
     1861 gctggcctgc gtgcgccaag tccgcgataa tgtgcaccct ttcgaccatc gctctcgggg
     1921 aatagatgct cgacattcag ttattgcgtg gcgatctcgc cgcgacggcc caacgcctcg
     1981 cgagccgggg ctttcagctc gacacgacgg cgtttcaggc gctcgaagcg gaacgcaaat
     2041 cggtgcaggt cgagacgcag gcgctgcagt cgcggcgcaa tgcactgtcg aagcagatcg
     2101 gtcagctcaa ggcgaagggc gaggatgcat ccgttctcat ggcgcaggtg aatgcgcagg
     2161 ccgaggagct gaagacgctc gagcagcggc tgagcgacgt ccagcagaag ctcaacgact
     2221 ttctgctcgt cgttcccaac ctcccgcatg cgagcgtgcc c
//
LOCUS       DASURG010000173         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_644597, whole genome shotgun sequence.
ACCESSION   DASURG010000173 DASURG010000000
VERSION     DASURG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644597"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1020
                     /locus_tag="VFY80_02895"
     CDS             97..1020
                     /locus_tag="VFY80_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6006879.1"
                     /translation="MTTAIASIAPAPPRPAGSETRDYERIARAIEYLRREAPAQPDLA
                     AVARHVHMSEYHFQRLFTRWAGVSPKRFLQYLTLEAAKGRLVRTGSLANVADDVGLSG
                     TGRLHDLFATLEAVSPADFRSGGNGLAIRYGIHASPFGEVSIATTKRGVCGVHFVERS
                     GEEMEVLRRQWPAARLIRDASGTAELAGRLFDPLARCGDRPLALVVKGTNFQLQVWRA
                     LLAVPLGRVTTYSELAKRIGHPDSPRAVGNAVGANPIAWLIPCHRVIRESGAFGNYRW
                     GERRKAAMLGWEAARCESLPEDKRASSARFR"
     gene            1017..1445
                     /locus_tag="VFY80_02900"
     CDS             1017..1445
                     /locus_tag="VFY80_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6006880.1"
                     /translation="MKDSLQPGLTFRFEFKVPASKTVPHLYPESAMFQDMPHVLATGY
                     LVGLMEWACIEALRPHLDWPREQSLGTHVNFSHLAATPPGLTVTVDVKLQKMEGRKLT
                     FSLSAHDGVDLISEGTHERIIIDAARFNSRIEQKTRQAAK"
     gene            complement(1586..>2233)
                     /locus_tag="VFY80_02905"
     CDS             complement(1586..>2233)
                     /locus_tag="VFY80_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6006881.1"
                     /translation="LGTRNIKVALRRLRRFAREGNPEELDLDDTVRSTAKNAGWLDLR
                     MVPERHNAVKVLLFLDVGGSMDDHVTACEQLFSAARSEFKRLEYFYFHNFLYERVWRD
                     NRRRATERVSTWDVMHTYPHDYKVIFVGDATMSPYEIVYPGGSVEHTNEEPGAVWMQR
                     LLTVYPNAIWLNPQPESLWDYHESIRMTRELLGERMFPLTIEGLDRGMRLLTKSH"
BASE COUNT          410 a          737 c          696 g          390 t
ORIGIN      
        1 cctacattag gaggggtggc accgaaggtg acggggtggt gaactcagca agaatcgggt
       61 ggcgcacgct ccgcgcgcgt tctatccttc gccatcatga cgaccgcaat cgcaagcata
      121 gcgccggccc caccgcgccc cgcaggcagc gaaacccgcg attacgagcg catcgcccgc
      181 gcaatcgaat acctgcgccg tgaggcgccc gcccagccgg atctcgccgc agtcgcgcgc
      241 cacgtccaca tgagcgagta tcactttcag cggcttttca cgcgctgggc cggcgtgagc
      301 ccgaagcgct tcctccagta cctgacgctt gaagcggcca aggggcggct cgttcgaacc
      361 ggcagcctcg ccaatgttgc cgacgacgtg ggactttccg gcaccggacg cctgcacgac
      421 cttttcgcca ccctggaagc ggtatccccg gctgacttcc gcagcggcgg caatgggctc
      481 gcgatacgtt acggcatcca cgcctcgcct ttcggtgagg tctcgatagc caccaccaag
      541 cgcggagtct gcggcgtgca tttcgtggag cgcagcggcg aagagatgga agtgctgcgg
      601 cggcagtggc ccgcggcacg actcatacgc gatgcctcgg gaaccgcgga actggccggg
      661 cgtcttttcg atccgctcgc ccgctgcggc gaccggccac tcgcgttggt cgtgaaaggc
      721 acgaacttcc agcttcaggt ctggcgcgcg ctgctcgcgg tgccgcttgg ccgcgttacg
      781 acgtacagcg aactcgcgaa gcgcatcggt caccccgatt cgccacgggc ggtcggcaat
      841 gcggtcggcg ccaatcccat cgcgtggttg atcccgtgcc atcgcgtcat acgcgaaagc
      901 ggcgccttcg ggaactaccg ttggggcgag aggcgcaagg ccgccatgct cggttgggaa
      961 gccgcccggt gtgaatcgct ccccgaggac aagagggctt catccgctcg gttccgatga
     1021 aagacagcct acagcccggc ctcacgttcc ggttcgagtt caaggtcccc gcgagcaaga
     1081 cggtgccgca tctctatccc gagtccgcga tgttccagga catgccgcac gtcctcgcca
     1141 ccggctacct cgtaggattg atggaatggg cgtgcatcga agcgctccgc ccgcatctcg
     1201 actggccgcg cgagcaatcc ctcggcactc acgtcaattt ttcgcacctc gccgcgacgc
     1261 caccggggct gacggtcacc gtcgatgtga aactccagaa aatggagggc cgcaagctga
     1321 ccttctcgct ttccgcccac gacggcgtgg atctcatttc ggaaggcacg cacgagcgca
     1381 tcatcatcga cgcggcgcgc ttcaattcga gaatcgaaca gaagacaagg caggcggcga
     1441 aataaaacca cgcgcacgag gagcgcgcgc acgtaacggc tcgatcggct tcccagcgga
     1501 tcgttttccc agtggattgt cttccgcgtg gcccgtcttc cgagcggatc gtcttcccag
     1561 cgaaagctgg gatccagaag aagaactagt gcgatttagt cagtaagcgc atgccgcggt
     1621 cgagcccctc gatcgtcagc ggaaacatgc gctcgcccag cagctcgcgc gtcatgcgga
     1681 tcgattcgtg gtaatcccac agcgactccg gctgggggtt gagccagatc gcattgggat
     1741 acaccgtcag gagccgctgc atccacaccg cccccggctc ctcgttggta tgctcgacgc
     1801 tgcctccggg atagacgatc tcgtacgggc tcatcgttgc atcgccgacg aagatgacct
     1861 tgtaatcgtg cggataggta tgcatcacgt cccacgtcga gacgcgctcg gtcgcccggc
     1921 gacggttgtc gcgccagacg cgctcgtaga gaaaattgtg gaagtagaag tattcgagac
     1981 gcttgaactc gctgcgcgcg gccgagaaaa gctgctcgca cgcggtgacg tgatcgtcca
     2041 tcgagccgcc gacgtcgagg aacagcagca ccttcaccgc gttgtgccgc tcgggaacca
     2101 tcctcaggtc gagccagccg gcgtttttcg cggtggagcg aaccgtgtcg tcgagatcga
     2161 gttcctcggg attgccttca cgcgcgaagc ggcgcagccg cctcaaggcc accttgatgt
     2221 tgcgggtgcc gag
//
LOCUS       DASURG010000174         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1462199, whole genome shotgun sequence.
ACCESSION   DASURG010000174 DASURG010000000
VERSION     DASURG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462199"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFY80_02910"
     CDS             <1..627
                     /locus_tag="VFY80_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006882.1"
                     /translation="DFSGVAVFGVVPNVTLISPSRGIKTLKELIEAAKKRPMTFASAG
                     VGSATHWAAERLLLSAGVKATHVPYKGGPEALTDVMTGRVDFMSTGIGSGLSFIRSGK
                     LIPLGVSTVKRSSALPDVPTTIEAGYPNSDFSFWNGLLVPAKTPRSIVTRLHAETQKV
                     LRSPAVVDKFKPLAMEPMPLTPAEFDALIKKEIENNIALAKAVGLKFN"
     gene            813..2060
                     /locus_tag="VFY80_02915"
     CDS             813..2060
                     /locus_tag="VFY80_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX6006883.1"
                     /translation="MPVPATVVSPDSSLARTAIRRPASLKGPQCHTLAVGDQPVLVYE
                     DELWTSKQRQANAIHEISYRACFKPQLPAYFIERLTQAGDVVYDPFSGRGTTAVEAAL
                     LGRTVIANDANPLSAILTYPRLELPCVSDVDKRLRSISFPATASADRDLSMFFHERTL
                     YEILGLRQYLARRRSNGAEDSIDRWIRMVATNRLTGHSPGFFSVYTLPPNQAISPENQ
                     VKINEKLGQKPEYRDVRALILRKSMQLQGALTAAERWRLHAAAETAQILVRDAGSTPA
                     IYSESVQLTVTSPPFLNVVQYARDNWLRCWFNGFDAEAVGERITMCATVEDWREAMAA
                     VLRELYRVTRRGGWVAFEVGEVRKAEVKLEEIVAPLGLEAGFECTAILINSQRFTKTA
                     NIWGIHNNRSGTNSNRIVLFRKP"
     gene            complement(2215..>2454)
                     /locus_tag="VFY80_02920"
     CDS             complement(2215..>2454)
                     /locus_tag="VFY80_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX6006884.1"
                     /translation="EAVKRDELPAGTREGLNDWKRTGYGGPCPPIGRHRYFHKLYALD
                     TVLPDLEHPNKAALEQAMRGHILAHVELIGTYQKS"
BASE COUNT          472 a          792 c          755 g          435 t
ORIGIN      
        1 gacttttccg gagtcgccgt cttcggcgtc gtgcccaatg tgacgctgat ctcgccgtcg
       61 cggggcatca agacgctcaa ggaactcatc gaagcagcaa agaaacgccc gatgacgttt
      121 gcctcggccg gcgtcggaag cgccacccac tgggcggccg agcgcctgct cctgagcgcg
      181 ggcgtcaagg cgacgcatgt tccctataaa ggcggcccgg aggcactcac cgacgtgatg
      241 accggccgcg tggatttcat gtccaccggc atcgggtcgg ggctcagttt cattcgctcg
      301 ggcaaattga ttccactcgg tgtcagcaca gtgaaacgct cgagcgcgct gcccgatgtg
      361 ccgacgacca tcgaggccgg ctacccgaac agcgacttca gcttctggaa cggcttgctg
      421 gtgccggcca agacgccgcg cagcattgtc acccggttgc atgcggaaac acagaaagtg
      481 ctgcgctcgc cagccgtggt cgacaagttc aagccattgg caatggagcc gatgcccctc
      541 acaccggcgg aattcgacgc gctgatcaag aaagagatcg aaaacaacat cgcgctcgcc
      601 aaggcggtgg ggctgaagtt caactaatac ccgcgcgctt gggtcaggag ggatgacaca
      661 aagcgcccga agtggtcttt ccgctcctac gttaggacag gtgggagcaa tagcattggc
      721 ggttccctgc taagggtcta ggtcagggct tgtacttttt ttcaaagggg cgtacgatcg
      781 cgcagcagta caacactcag aaggagtgcg cgatgcccgt acctgccacc gtcgtctcac
      841 cggattcatc gcttgcccgt actgcaatcc ggcgccctgc atcgctgaag gggccgcaat
      901 gccacaccct cgccgtcggc gatcagccgg tcctcgtata cgaagacgag ctgtggacga
      961 gcaagcagcg ccaggcgaat gccattcacg aaatctcgta ccgcgcctgt ttcaagccgc
     1021 agctgcccgc ctacttcatc gagcgcctca cgcaggcggg cgatgtcgtg tacgacccgt
     1081 tcagcgggcg cgggacaacg gcggtggaag ccgcacttct gggacgcacc gtaatcgcga
     1141 acgacgcgaa cccgctgtcg gcgatcctca cctatccgcg tctcgaattg ccgtgcgtgt
     1201 cggacgtgga caagcgcctg cgctcgatca gcttccccgc gaccgcttcc gccgatcgcg
     1261 atctctcgat gttctttcac gagcgtacac tctacgaaat tctcggtctg cgccagtacc
     1321 tcgcgcggcg ccgtagcaac ggcgcggagg acagcatcga ccgctggatc cgcatggtgg
     1381 cgaccaaccg cctcacgggc cactcaccgg gattcttttc cgtgtacacg ctgccgccca
     1441 accaggcgat ctcgcccgag aatcaggtga agatcaacga gaagctggga cagaagcccg
     1501 aatatcgcga cgtgcgcgcg cttatcctgc gcaaatccat gcagctgcag ggcgcgctca
     1561 cggccgccga acgctggcgc ctgcacgcgg cagcggaaac ggcgcagatt ctcgtgcgcg
     1621 acgccggatc gacccctgcc atctattccg aaagcgtgca gctcacggtc acctcgccgc
     1681 ccttcctgaa cgtcgtgcag tacgcgcgcg acaactggct gcgctgctgg ttcaacggct
     1741 tcgacgccga ggccgtcggc gagcgcatca cgatgtgcgc caccgtcgaa gactggcgcg
     1801 aggcgatggc ggcagtgctc cgcgaactct atcgcgtcac gcggcggggc ggctgggttg
     1861 cgttcgaagt gggcgaagtg cgcaaggccg aagtcaagct cgaggagatc gtggcaccgc
     1921 tcgggctgga agccggattc gaatgcaccg cgattctcat caacagccag cgcttcacca
     1981 agacggcaaa catctgggga atccacaaca accggagcgg aacgaactcg aaccggatcg
     2041 tgcttttccg caagccgtag attcgcttgg cctggatccc ggcttgcgcc gggaagacga
     2101 ccttatttcg tcatcccgcc acgatgactg cacgcgccgg ggtgacgacc tcgtttcgtc
     2161 attccgttga aaaacggaat ccagtcaggc gtgggtacgc tccgtcgcgc gcaatcagga
     2221 tttctggtag gtgccgatca actcgacgtg cgccagaatg tggccgcgca tcgcctgctc
     2281 gagcgccgcc ttgttcgggt gctcaaggtc gggcaacacg gtgtcgagcg cgtagagctt
     2341 gtgaaaatag cggtgccggc cgatgggcgg acacggccca ccgtagccgg tgcgtttcca
     2401 gtcgttgagg ccctctcgcg tgcctgccgg aagctcgtcg cgcttcaccg cttc
//
LOCUS       DASURG010000175         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_133345, whole genome shotgun sequence.
ACCESSION   DASURG010000175 DASURG010000000
VERSION     DASURG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133345"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFY80_02925"
     CDS             <1..804
                     /locus_tag="VFY80_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6006885.1"
                     /translation="GSGELGVPVNVSGPEDLQYLGERLDWMRRRLLDLEQQKNRFLRH
                     VSHELKTPLTAVREGAELLSEEVVGKLEPRQREIAEILRHNSIELQRLIEDLLSYSAS
                     QFHKVTLDLRPVQIRRVIDRVADDLALALGAKDLRLEVTTDSVTLNADFEKLRIILDN
                     LMSNAIKFSPAGGTILVTARVIGGYLEIDVADEGPGIPHADRAHVFDPFYQGKQAVDA
                     LVKGTGIGLSVVKEYTQAHGGTVEALDRGTRPGALLRVRLPLGRMEAAA"
     gene            816..1397
                     /locus_tag="VFY80_02930"
     CDS             816..1397
                     /locus_tag="VFY80_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006886.1"
                     /translation="MAGIALIIAGCVTPPPEEPPAPVSVAKPETGPPAEAPDTEARAQ
                     PAIIAPPVEPPPRPRADDVERLLVYFEHVRKLPAPELGRENEIARAAFNRTRSEFDRV
                     RLAMLLSVPNTAIADEQRAYDLLDPVVKNPTSPLHGLALLMTTNLQERRRLESNMQQL
                     QQNVQGLQNKLDALMSLERTLIDRDKSPPQRKR"
     gene            1394..2773
                     /locus_tag="VFY80_02935"
     CDS             1394..2773
                     /locus_tag="VFY80_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6006887.1"
                     /translation="MTEMTLPVRANILVVDDDPDLLRLMQIRLAAAGYGVATADSAEH
                     ALAQLTVARPRVVVTDLRLGGMDGIALFEAIHKENPSLPVIILTAHGTIPDAVTAVKR
                     GVFGYLTKPFDAKELLGEVERALALTGGRETVEKGADDQGWRKEIITRNPVMEDMLVK
                     ARLVADSDASVLIYGESGTGKELLARAIHHASPRRDRPFVAINCGAIPEQLLESELFG
                     HVKGAFTGATRDHKGLFQTADKGTLFLDEIGDMPLTLQVKLLRALQERQVRPVGSTFT
                     YDIDVRLISATHRNIEAEMLAGNFREDLFYRLNVVNLTLPPLAERREDIPLLASHFLA
                     TLAEKYRKKINGFAPEALELLVGAAWPGNVRQLYNVVEQSVALATTPLIPPTRVQQAI
                     QNQQREFASFEEARKKFERDYLAQLLKITEGNVTQAARLAKRNRTEFYKLLQRHQLDP
                     KLFKAAARQ"
     gene            3029..3214
                     /locus_tag="VFY80_02940"
     CDS             3029..3214
                     /locus_tag="VFY80_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006888.1"
                     /translation="MMKTAFNEAFAKQVAPRTLLPAEACSELGVASARTRVVKRLPRW
                     LPVFRRWLAGRYPGLHG"
     gene            3243..3545
                     /locus_tag="VFY80_02945"
     CDS             3243..3545
                     /locus_tag="VFY80_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006889.1"
                     /translation="MNGSDAVRRTMDTDERHDRHGGMLYALLLVAAIAVIVFSIIGIA
                     TMTGVVSNAAGDAALRPSGGAAQPGRPQRLEAPQREPLPQPGNPRTAAISQATVAL"
BASE COUNT          771 a         1336 c         1263 g          636 t
ORIGIN      
        1 ggaagcgggg agctcggggt gccggtgaac gtgagcgggc cggaggatct gcagtatctg
       61 ggcgagcgtc tcgactggat gcgacgacgc ctgctcgacc tcgagcagca gaagaaccgc
      121 ttcctgcgcc atgtctcgca cgaactgaag acgccgctca cggcggtgcg cgaaggcgcc
      181 gagctgcttt cggaggaagt ggtgggcaaa ctcgagccgc ggcagcgcga aatagcggaa
      241 atcctgcgcc acaacagtat agaattgcag aggctgatcg aagaccttct gagctatagc
      301 gcaagccaat tccacaaggt cacgcttgac ctccgtccgg tacagatccg acgggtgatc
      361 gaccgggtcg ccgacgatct cgcgcttgcc ttgggggcaa aggacctccg gctcgaggtc
      421 accacggaca gcgtcaccct caacgcggac ttcgagaagt tgcgcatcat tctcgacaac
      481 ctcatgtcca atgccatcaa gttctcgccg gccggaggaa cgatactcgt gaccgcgcgc
      541 gtgatcggcg gctatctcga gatcgacgtt gcagacgaag ggccgggcat cccgcatgcc
      601 gaccgcgcgc acgtcttcga tcccttctat cagggcaagc aggcggtgga tgcgctggtg
      661 aaaggcaccg gcatcgggct ttccgtggtg aaggagtaca cccaggcgca cgggggcacc
      721 gtcgaagcgc tcgaccgcgg cacgcgcccg ggcgcgctgc tccgcgtgcg cttgccgctc
      781 ggtcggatgg aggccgccgc atgaggtgct ggagcatggc cggcatcgct ctcatcatcg
      841 caggctgcgt gacgccaccg cccgaagagc cgccggcgcc ggtgagcgtc gcaaagccgg
      901 aaacgggtcc gcctgccgaa gcgcccgata cggaagcgcg cgcgcaacct gcgatcatcg
      961 ccccgcctgt ggagccgccg ccgcggccgc gtgccgacga cgtcgaacgc ctgctcgtgt
     1021 acttcgagca cgtgcgcaaa ctgcccgcac cggaattggg ccgcgagaac gagatcgcgc
     1081 gcgcagcgtt caatcgcacc cggtccgaat tcgatcgcgt gcggcttgcc atgctgctct
     1141 ccgttccgaa tactgcaatc gccgacgagc agcgcgctta cgatctgctc gatccggtcg
     1201 tgaagaatcc cacctcaccg ctccatggtc tcgcgcttct catgacgacg aacctgcagg
     1261 agcgccgcag gctggagagc aacatgcagc agctccagca aaacgtccag ggcttgcaga
     1321 acaagctcga cgccctcatg tcgctcgaac gcacgctgat cgatcgcgac aaaagtccgc
     1381 cccagaggaa acgatgactg aaatgaccct tcctgtacgc gcgaacatac tcgtggtcga
     1441 tgacgatccg gatcttctgc ggctcatgca gatccggctc gcggccgccg gatacggcgt
     1501 cgctacggcg gacagcgcgg agcacgcgct cgcgcaactc accgtggcgc ggccccgggt
     1561 ggtggtgacc gaccttcggc tgggcgggat ggacggcatc gcgctcttcg aagcgatcca
     1621 caaggagaat ccctcgttgc cggtgatcat tctcaccgcg cacggcacca ttcccgacgc
     1681 cgttacggct gtgaaacgcg gcgtattcgg ctatctcacg aaaccgttcg atgcgaagga
     1741 actgctgggc gaagtggagc gcgcgctcgc gctcacaggc ggacgggaga cggtcgagaa
     1801 gggggccgac gatcagggct ggcgcaagga gattatcacc cgcaacccgg tgatggaaga
     1861 catgctggtg aaagcgcggc ttgtcgccga cagcgacgcg agcgtcctga tctacggaga
     1921 gtcgggcacc ggcaaggaac tgctcgcgcg cgcgatccac catgcgagcc cgcggcggga
     1981 ccgaccgttc gtcgcgatca actgcggcgc gattcccgag cagttgctcg aatccgagct
     2041 cttcgggcac gtgaaaggtg cgtttaccgg ggcgacgcgc gaccacaagg ggctgtttca
     2101 gacggccgac aagggcacgc tctttctcga tgaaattgga gacatgccgc tcacgctgca
     2161 ggtgaagctg ctacgtgcgc tccaggaacg gcaggtccgt ccggtcggct cgaccttcac
     2221 ctacgacatc gatgtgcgcc tgatctctgc cacgcaccgt aacatcgagg ccgagatgct
     2281 cgccgggaat ttccgcgagg atctcttcta ccgcttaaac gtcgtcaatc tcacgctgcc
     2341 gccgctggcc gaacgccggg aggacattcc gctgctcgcc agtcacttcc tcgccacgct
     2401 cgccgaaaag tacagaaaga agatcaacgg ctttgcgccc gaggcgctgg agctgctggt
     2461 cggcgccgcc tggccgggca acgtgcgtca gctctacaac gtggtggagc agtcggtcgc
     2521 gctcgccacg acccctctca tccccccgac ccgggtccag caggcgattc agaaccagca
     2581 gcgcgagttt gcgtccttcg aggaggcgcg caagaaattc gagcgcgatt acctggcgca
     2641 actgctcaag atcaccgaag gcaacgtcac gcaggctgcc cggctcgcca agcgcaaccg
     2701 gaccgagttc tacaagctcc tccagcgcca tcagctcgat cccaagctct ttaaggcggc
     2761 tgcgcgccag tagcgggtgt cgccgcgccg cgacaataca acctgcggca aatcaacccg
     2821 ctagtggcgc ggggcggcag cttgtggttg aacagcgaca aggacccccc gctgccgacg
     2881 ccccaactca ctgatttggc tgcatagcga ccgtcggcac gacgcttgct tcgcatacga
     2941 cgcgcgtagg aagggtacgt cgcgtacgtc tccctccggc tgacagcgca ggcaattgga
     3001 cgaacggcgc caccacggga gaagcaccat gatgaagacg gcattcaacg aggcgttcgc
     3061 gaaacaagtc gcaccccgca cgctgctccc cgcagaagcg tgcagcgagc tcggcgtcgc
     3121 gtccgcccgc acccgcgtcg tgaagcgtct cccgcgttgg ctgccggtgt tccggcgctg
     3181 gctcgccggc cgatacccgg gtctgcatgg ttgaccataa cgaaccaagg gaagaacagg
     3241 cattgaacgg ctccgatgca gtgaggcgca ccatggatac cgatgagcgt cacgaccgcc
     3301 acggcggcat gctctacgcg ctgcttcttg tcgccgccat cgcggtgatc gtgttcagca
     3361 tcatcggcat cgccacgatg accggagtcg tgtcgaacgc agccggcgac gcagcactgc
     3421 gcccgagcgg tggcgcggcg cagcccggtc gtccgcagcg gcttgaagcg ccgcagcgtg
     3481 aaccgctgcc gcagcccggc aaccctcgta ccgcagccat ttcccaagcg acagtcgcac
     3541 tttaagaaaa tcccgacgct cgcgggagta tcatgcgcgg aagcaaaagg accaacgcat
     3601 gcgtatcccc gctctcgccc tggcattgac attcatcgcc gccggttgcg ccaccgcgcc
     3661 gcgcagcgac gctccggttc cgcgcggcga tccggcgtcg gctccgagcc aaccttcagt
     3721 ccagcggccc tcgcccccgg cgcctgtcaa tctttccgga tactcgccgg cattcaagca
     3781 gggctacagc gacggctgcg cgagtgcgcg cgggtcgatg cgccgtgatg acgcgcgcta
     3841 tcgcagcgac aacgactaca tgatgggttg gagcgacggg aacagcgtct gccgcgcgcg
     3901 caggtaagcc tcgcacgcgc tcgcgcgtga tgcctcgatc gcctactctt gaggtaggcg
     3961 catcgctaca accgcttaaa taaagtgcgt ttgataaggt cgaaac
//
LOCUS       DASURG010000176         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_542121, whole genome shotgun sequence.
ACCESSION   DASURG010000176 DASURG010000000
VERSION     DASURG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542121"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFY80_02950"
     CDS             <1..636
                     /locus_tag="VFY80_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6006890.1"
                     /translation="TDKPIRYLVYSHHHFDHIAGGKPFKDAGARIIAHKRVQERLAIL
                     KDPATVTPDEVFGNQRTLQLGGTTLELTHIGLNHSDSSIVMRLPREKIVFAVDFIPVG
                     SISGFAMIDSYPLEWEASLKKLAAMDWERLIPGHPGQPGGRLGTKKDVQEYLDFLQYA
                     SAEVKEVARNGKCWQPAEKEVTLSKYSSWPGYEMARPYVVRRYCGLWGRGT"
     gene            complement(606..1457)
                     /locus_tag="VFY80_02955"
     CDS             complement(606..1457)
                     /locus_tag="VFY80_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6006891.1"
                     /translation="MPWTFEWLVVACLLIAALLYLAGAWKLWRKAGVGRGLTLTHAGA
                     FTAGWLTLVVALASPLDALGARLFSAHMVQHELLMVVAAPLFVLSRPLEAWTWGLPPA
                     WRGAAGRVAHWRPLQLMWGTVTAAFPAWAIHAAALWLWHVPAFFNAALSSEGMHILQH
                     ASFLASALLFWWAALGRTGERSPALAIALLFTTMLHTGALGALLTFSTSPWYRAYGNT
                     GDFGLAAIEDQQLGGLIMWIPGGVAYLASGLAIVFRHYLAAAADGARPPRPALSGDQV
                     PRPHRPQ"
     gene            1703..2683
                     /locus_tag="VFY80_02960"
     CDS             1703..2683
                     /locus_tag="VFY80_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006892.1"
                     /translation="MAVNKILACACMSVAWCGVMGTAHAAATRYPDKPIRMIVPWNPG
                     GTSDTIARILGQKMTETWGQQVVIDTRAGASGIIGTEIAMRAPADGYTLLHANMSQWA
                     TNPSLYKVNYDTLRDFAPVSMVATAPQLLVVHPGVAAKSVQELIALAKAQPGTLNFGS
                     GGAGTLAYPAGEMFKAMAGVNLTHIPYKGTILALNDLMAGRVQVVFSDMPIALPHAKS
                     GKLRALAVTSAKRTPLVPDMPTVAEAGVPGYAIDNSWGVFARRAVSPDILAKLNAEIV
                     RVHELRDVRERYAALGLEAVSSTPQRFAQVIREDAAKLSKIIKATGAKVD"
BASE COUNT          544 a          897 c          933 g          419 t
ORIGIN      
        1 accgacaaac cgatccggta cctggtctac agccaccatc acttcgacca tatcgcaggc
       61 ggcaagccgt tcaaggatgc gggtgcccgg atcatcgccc acaagcgcgt tcaggaacgt
      121 cttgccatcc tcaaagaccc ggccaccgta acgcccgatg aagtcttcgg caaccagcgc
      181 acgctgcagc tcggcggcac gacgctggag ctcacccaca tcgggctcaa tcactccgac
      241 agctcgatcg tgatgcggct gccgagggag aagatcgtat tcgcggtgga tttcattccg
      301 gtcgggtcga tctcgggctt cgcgatgatc gactcctatc cgctcgagtg ggaagcctcg
      361 ctcaagaaac tcgcggcgat ggactgggag cgcctgattc ccggccatcc cggacaaccg
      421 ggcggccgtc tcggcaccaa gaaggatgtc caggagtatc tcgacttcct gcagtacgcc
      481 tcggcggagg tcaaggaggt cgcgcggaac ggcaagtgct ggcagccggc cgagaaggaa
      541 gtgacgctgt ccaagtattc gagctggccg ggttatgaga tggcgcgccc gtacgtggtg
      601 cgccgctatt gcggcctgtg ggggcgcggc acctgatccc ccgacaacgc cggacgtggc
      661 ggccgcgctc catcggccgc cgcggcaaga tagtgccgga atacgatcgc gagccccgag
      721 gcgaggtagg cgaccccgcc gggtatccac atgatgagac cgccgagctg ctgatcctcg
      781 atcgcggcga gtccgaaatc cccggtgttg ccgtacgccc ggtaccaggg cgatgtcgag
      841 aaggtgagga gggcgccgag cgcccccgtg tgcagcatgg tggtgaaaag gagcgcaatc
      901 gcaagagcag ggctacgctc ccccgttctc cccagcgccg cccaccagaa aagcagcgcg
      961 gaggcgagaa agcttgcgtg ctgcaggata tgcatcccct cgctcgacag cgcggcgttg
     1021 aaaaacgccg gcacgtgcca gagccacagt gcggccgcgt gaatcgccca agccgggaat
     1081 gcagcggtca ctgtacccca catgagctga agtggccgcc agtgcgcgac gcggcccgcg
     1141 gctccgcgcc acgcgggcgg caaaccccag gtccaggctt cgagcggccg tgacaggacg
     1201 aagaggggcg ccgcaacgac catcagcagt tcgtgttgca ccatgtgggc cgagaaaagc
     1261 cgtgcgccca gcgcgtcgag gggcgaggcg agcgcgacga cgagcgtgag ccagcccgcg
     1321 gtgaatgcgc ccgcgtgcgt aagcgtgaga ccgcgaccga ctccggcctt gcgccacagc
     1381 ttccatgcgc cggcgagata gagcaacgcc gcgatcagca ggcacgcaac gacaagccac
     1441 tcaaacgtcc atggcaaagc tggcgcctcg ggcacggtat gcgcgagggc agggcggaga
     1501 ggcaacacgc agaaagcccc cgcgaagggc aagcgcatat gcacggtgaa tgaggcgggt
     1561 gtcggttgga agcttcagcc gttgagacca cggaagcggc gcagagtgcc agcgttccag
     1621 tgcggcgatt gagccgtctc gtgcttgttc acggcgctct ttgcactaca atcgcatgcc
     1681 cccaatcacc gagaggtctc gaatggcagt gaacaaaatt ctggcatgtg cgtgtatgtc
     1741 tgtggcttgg tgcggcgtga tgggaacggc gcacgccgca gccacgcggt atccggataa
     1801 accgatacgc atgatcgtgc cgtggaaccc cggcggcacg tccgacacca tcgcccgcat
     1861 cctgggccag aaaatgaccg agacgtgggg acagcaggtc gtgatcgaca cgcgcgccgg
     1921 cgcgagcggc atcatcggaa cggagatcgc catgcgggcg cccgcggacg gctacacgct
     1981 gctccatgcc aacatgagcc agtgggcgac caatccgagt ctctacaagg tgaactacga
     2041 cacgctgcgc gatttcgcgc cggtcagcat ggtggcgacc gcgccccagc ttctcgtcgt
     2101 gcatcccggt gtcgcggcca aatcggtgca ggagctgatc gcgctcgcga aagcgcagcc
     2161 gggcacgctc aacttcggct ccggcggcgc gggcacgctc gcgtatccgg cgggggaaat
     2221 gttcaaggcg atggcgggcg tgaacctcac gcacattccg tacaagggca cgatactcgc
     2281 gctgaacgac ctgatggcgg gccgggtgca ggtggtgttc tcggacatgc cgatcgcgct
     2341 gccgcatgcg aaatcgggga agctgcgcgc gcttgcagtc accagcgcga aacgcacgcc
     2401 gctcgtcccc gatatgccga ccgtcgcgga agcgggcgtg cccggctacg ccatcgacaa
     2461 ttcctggggc gtctttgcgc ggcgcgcggt ttcgcccgat atccttgcca agctcaacgc
     2521 ggaaatcgtt cgcgtgcacg agctgcgcga cgtgcgcgag cgctatgcgg ctctgggcct
     2581 ggaggcggtg agcagcacgc cgcaaaggtt cgcacaggtc atccgcgagg acgcggcgaa
     2641 gttatcgaag atcatcaagg cgacgggcgc gaaggtcgat tgacgcacgg ctctccttga
     2701 accgtcgggc gctcatcgca gcgcagcggt agaatgggcc catgaagccg cgcctttttc
     2761 tccttcccca agcgccttcg tgattccccg gaa
//
LOCUS       DASURG010000177         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_31927, whole genome shotgun sequence.
ACCESSION   DASURG010000177 DASURG010000000
VERSION     DASURG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31927"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VFY80_02965"
     CDS             <1..1274
                     /locus_tag="VFY80_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6006893.1"
                     /translation="MEMQAVHSDEFQPWAQLAASLAIGFLIGLERQRNPTAKAGVRTC
                     ALVALFGAIAALLSQALGTAWILAAGLLLSGAMIIAAYVGPENHEADSGTTTVIAVLL
                     CYCLGAMVWFGYMKFAVALAIAITVLLHFKAQLHEFSEKLAPHDISMMLQFAVLSFIV
                     LPLLPDEGYGPYRALNPYHTWLMVVLVSGLSVAGYLALRVLGGRRGLLVVGLAGGLVS
                     STATTVVQSRQAGLRAEWSAASASVIAISNLVVLARLSVLALVSAPAIVPLLLPALAA
                     GVACGLPTAIRRFRGATSGSAVAVPEVSNPASVPVALGFGALYAAVLLLSAWLSDRAG
                     EGALLGLAAVSAVVDLDAITLSTLRLFSTGVVAADIAATAVIVAFLAASLSKLAIIGW
                     LGGARLVRLSWLPLLAPVVGAAVVLWVMPKG"
     gene            1322..1906
                     /locus_tag="VFY80_02970"
     CDS             1322..1906
                     /locus_tag="VFY80_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEX6006894.1"
                     /translation="MTLDVGKGDALLVVDVQRDFLPGGNLAVHEGDAVVPVLNRYIDL
                     ARKKGLPILASRDWHPERHCSFKEQGGPWPPHCVADTPGAQFPKDLQLPPGTVVIDKA
                     TRPEVDAYSAFSGTTLADRLRALGVKRVLIGGLTTDYCVVNSVRDALREGFEVVVLAD
                     AIRAVNVNPDDGENAQREMWSRGAVPARYEDLAA"
     gene            1918..>2916
                     /locus_tag="VFY80_02975"
     CDS             1918..>2916
                     /locus_tag="VFY80_02975"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX6006895.1"
                     /translation="MNLGSPLLTDLYQLTMLQAYFARGMQDTAVFEFFVRKLPPNRNF
                     LMAAGLEQTLDFLEGLRFSGEEIDWLAKSGRFSREFVSRLEGLRFTGDVHAMPEGTIF
                     FANEPILRVTAPLPEAQFVESRIINLLQYQTLVASKAARCVLTAPGRLLVDFGLRRAH
                     GAEAGLYAARAAYIAGYAGTATVLAGARFAIPLYGTMAHSYVQAHDDEAEAFRDFARA
                     FPGGTVLLVDTYDTEAAVRKIVALAPQLQREGLSIRGIRLDSGDLAALAKRARQLLDQ
                     AGLTDVTIFASGNMDEYRLAEILAAEAPIDGFGIGTSLTTSSDAPYLDAVYKLQEYA"
BASE COUNT          431 a          960 c          963 g          562 t
ORIGIN      
        1 caatggaaat gcaagccgtt cattccgatg agtttcagcc ctgggcgcag cttgccgcga
       61 gccttgcgat cggatttctg atcgggctcg aacgccagcg caatcccacc gcgaaggcgg
      121 gcgtgcggac ctgtgcgctg gttgcgctgt tcggtgcgat cgccgcgctg ctctcgcagg
      181 cgctcgggac ggcatggata ctggcagcgg gtttgctgtt gtcgggcgcg atgatcatcg
      241 cggcctacgt cgggcccgag aaccacgaag ccgactcggg cacgacgacg gtcatcgccg
      301 tcctcctctg ctattgcctc ggggccatgg tgtggttcgg ctacatgaag tttgccgtgg
      361 cgctcgcgat cgcgatcacc gtgctgctcc acttcaaggc gcagctgcac gaattctcgg
      421 agaagctcgc cccgcacgac atcagcatga tgctgcagtt cgcggtgctg agcttcatcg
      481 ttttgccgct gttgcccgat gagggctacg gcccctatcg cgcactcaac ccctatcaca
      541 cgtggctcat ggtcgtgctg gtgtcggggc tgagcgtggc gggctatctt gcgctgcggg
      601 tactgggtgg gcgccgcggg ctcctcgtag tgggtctcgc cggcggactc gtttcgagca
      661 cggcgacgac cgtggtgcag tcgcgtcagg cgggcctgcg cgcggagtgg tccgcggcga
      721 gtgcgtccgt cattgcgatc tccaatctcg tcgtcctcgc acggctctcg gtgctggcgc
      781 tcgtcagcgc gccggcaatc gtgcccctgc tcctgccggc gctcgccgcc ggcgtggcgt
      841 gcgggctgcc gaccgcgatc cggcgattcc gcggcgccac gtcgggcagc gccgtggcgg
      901 ttccggaagt atccaatccg gccagcgttc ccgtcgcgct cggctttggc gcgttgtatg
      961 ctgccgtcct gctgctttcc gcatggttgt cggaccgcgc gggggaaggc gcgctcctgg
     1021 ggcttgccgc cgtatcggca gtggtggatc tcgatgccat tacgctttcc acgctgcggc
     1081 tcttcagcac cggcgtcgtt gccgcagaca tcgctgcgac ggccgtcatc gtcgccttcc
     1141 tcgcggcatc gctttcgaag ctcgccatca tcggctggct gggcggcgcg cggctcgtgc
     1201 gcctctcgtg gctgccgctg ctcgcgcccg tcgttggcgc ggcggttgtg ctgtgggtca
     1261 tgcccaaggg ttgaacccgg accgcgtcac tcgcctcaaa tgtgtgagga ggacgactca
     1321 gatgaccttg gacgttggaa aaggcgacgc gctgctcgtc gtcgatgtgc agcgcgattt
     1381 tctccccggt ggtaacttgg ctgttcacga gggcgatgcg gtcgtaccgg tgctcaaccg
     1441 ttacatcgat ctcgcgcgca agaaaggact gcccattctg gcaagccgcg attggcaccc
     1501 cgagcggcat tgctcgttca aggaacaagg cggcccgtgg ccaccgcatt gcgtagcgga
     1561 tacgcccgga gcgcagtttc cgaaagacct gcagctcccg ccgggtaccg ttgtcatcga
     1621 caaggcgacg cgtcccgagg tggatgccta ctcggctttt tccggtacga cgcttgccga
     1681 ccgattgcgc gcgcttggag tgaaacgcgt gctcatcggc gggctcacga ccgactattg
     1741 cgtcgtcaac tcggtacgcg atgcactgcg cgaaggcttc gaagtcgtgg tgctcgcgga
     1801 tgcaatccgc gcggtcaacg taaacccgga cgacggcgag aacgcgcaac gcgagatgtg
     1861 gtcgcgcggc gccgtgccgg cgcgttacga agatctcgcg gcgtgaaagc gcgcggcatg
     1921 aacctcggtt ccccgctgct gacggatctc taccagctca cgatgctgca ggcttacttc
     1981 gcccgcggca tgcaggatac cgccgtcttc gagttcttcg tgcgcaagct tccaccgaac
     2041 cgcaatttcc tgatggccgc gggactggag cagacgctcg attttctcga gggcctgcgc
     2101 ttcagcggcg aggagatcga ctggctcgcg aaaagcgggc ggttctcacg cgaattcgta
     2161 tcgcgtctcg aaggcctgcg ctttaccggc gacgtgcatg ccatgccgga aggcaccatt
     2221 tttttcgcga acgagccgat cctgcgcgtc accgccccgc tgcccgaggc gcagttcgtc
     2281 gaatcgcgca tcatcaacct cctgcagtat cagacgctcg tcgccagcaa ggccgcgcgt
     2341 tgcgtcctga ctgcgccggg caggctgctg gtcgatttcg gcctgcgccg cgcccacggc
     2401 gcggaggccg gcctgtatgc cgcgcgcgcc gcgtatatcg cgggctacgc gggaacggcg
     2461 accgtgctgg cgggcgcgcg cttcgccatt ccgctctacg ggacgatggc gcactcgtac
     2521 gttcaggcac acgacgatga agcggaggcc tttcgcgatt tcgcacgcgc gtttcccggc
     2581 ggcacggtat tgctcgtaga tacctacgat acggaggcgg cggtgcgcaa gatcgtcgcg
     2641 ctcgcgccgc agttgcagcg cgagggcctc tccatccgcg gcatccggct cgacagcgga
     2701 gacctcgccg cgctggcaaa gcgagcccgg cagctgctcg atcaagccgg cctcacggat
     2761 gtgacgattt tcgcaagcgg caacatggac gagtaccggc tggccgaaat cctcgccgcc
     2821 gaagccccga tcgacggctt tggcatcggc acgagcctca ccacgtcgtc cgacgcccca
     2881 tatctcgacg ccgtgtacaa gttgcaggaa tacgca
//
LOCUS       DASURG010000178         5114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_134230, whole genome shotgun sequence.
ACCESSION   DASURG010000178 DASURG010000000
VERSION     DASURG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134230"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFY80_02980"
     CDS             complement(<1..342)
                     /locus_tag="VFY80_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006896.1"
                     /translation="MEPIKLRFGGYQKTASIHNRAAQRFGERLIRTLGSTIAFDLVEN
                     VLDLGRKSGELPTMVETGELAGCYMSTVRFTAIVPELKVLELPFVVKNRATLFQALDG
                     NIGDRLRHRMLE"
     gene            complement(387..1247)
                     /locus_tag="VFY80_02985"
     CDS             complement(387..1247)
                     /locus_tag="VFY80_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6006897.1"
                     /translation="MRNDFILGLSAKGFHRVHYTEWGDAGNPRIVVCVHGLTRTGRDF
                     DDLARALQGEFRVACPDIVGRGLSDWLPASEDYGYPQYLADMNALIARLTAGAEHWEL
                     HWVGTSMGGIIGMLLAAQPRSPVRKLVVNDVGACIPKAALERIGMYLGRTPRFETLEA
                     LEAHLRRVSAPFGPLTDAQWRHLTVHAARQLDDGSWTTAYDPAIAAPFHEQPQHDIDL
                     WRYWDAIRCPTLLVRGADSDLLPREVATAMVARGPRPRLAEFSGVGHAPMLMSADQIA
                     AVREFLLEAT"
     gene            complement(1270..2157)
                     /locus_tag="VFY80_02990"
     CDS             complement(1270..2157)
                     /locus_tag="VFY80_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006898.1"
                     /translation="MALSHHRAEWLTGVIHFIILLIALKADSAEVWPWALSAMAIASF
                     FAWAATYRRYRHIHDNPTSKVASAAQGYVELFGHSELLPGNTLVSSRTGLPCCWRRYC
                     IDRKTSNDKWERVDSGESNDHFLLVDDTGECVISPEGAEILTERKDTWMQGNYRHTEW
                     LIVPKGRLYAIGEFSTAGGAISLLDERADVSDLLADWKKDRAQLLARFDRNKDGEIDL
                     PEWEHARLEARKEVRKRHAEGYAAVTEGVHLMRKPRDGRLFLLACALPERLGRRFAFW
                     STVHLVFFIGAGTAAIFMF"
     gene            complement(2182..2754)
                     /locus_tag="VFY80_02995"
     CDS             complement(2182..2754)
                     /locus_tag="VFY80_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6006899.1"
                     /translation="MVGTVIVLGALLVLVVYFVMIYNNLVQIKHNVSKAWANIDVLLK
                     QRHDELPKLVETCKQYMKFEQETLERVMEARSRVFAAREAQNIGALGQAEGMLRASLG
                     NLFALAEAYPDLKTNQSFQQLQARISSLENAIADRREFYNESVNINNVRIEQFPDTIL
                     AGMFGFKPAQLLEFDATEKADVDVKKLFAA"
     gene            complement(2809..4125)
                     /locus_tag="VFY80_03000"
     CDS             complement(2809..4125)
                     /locus_tag="VFY80_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX6006900.1"
                     /translation="MTGGSELRIEVICTGDEVLTGKIVNTNFSYISQKLEDFGLSVRW
                     ETTVGDDRESLLLAFRLAGERADAVIVNGGLGPTVDDLSQEIAARAAGVDLVLSDEWL
                     ARMEEYFRRRSRVMPANNRKQAMLPAGAEILDNPVGTACGFAVDIGRARFYFTPGVPR
                     EMRRMLEGEIIPRLLAKSGMQAAIFLKRFHSYGIGESHADALLAGVEALVPDGSVKLG
                     FRAHYPQLETKLTVRGKDMEDIKRKLAPVEAEVRRRLGNFILAEGDQTLEQVVLEALS
                     SRGATLALVETFTSGQIAARIGHLPGAESVIRRGLISRDLAQLCAALSLQPGIDGVTR
                     ESAEAVARAIRVQTGATHALAVLIEIDEGPDRIDIGGTVCIAIATASEIATRRSRIVG
                     GRDWVRLGAVEMGLDCLRRYVQGLPVQERIDFEKVEGATEATGAKA"
     gene            complement(4139..5029)
                     /locus_tag="VFY80_03005"
     CDS             complement(4139..5029)
                     /locus_tag="VFY80_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX6006901.1"
                     /translation="MDLNSDTHAGERVVFWYGCNVLRHGDIIHGCLDILKMLGIDARA
                     AGGPDYCCGTGKDDNQTAADGMARRTVTKFNAMAREKVIAWCPSCHAHMTEFMGRAYP
                     TDFGLTYLVDLLYERRDALAALLKHRVPIRVLLHKHVGFNDRVKVNEKVPALLRLIPG
                     LEVVNDDYAAPGYMCALLASVPKALEDMHRETLRRVALHKADAVVTTFHQCYREVVGL
                     EAHADTKAYNYIHLLAQSIGLQYADEYKAWKKAGPKAAELIGEKRLEKVGIQFFERAM
                     LPELVKRPLGIDRKPGTTED"
BASE COUNT          883 a         1640 c         1632 g          959 t
ORIGIN      
        1 ctccagcata cgatgccgca ggcggtcgcc gatgttgcca tcgagcgcct gaaagagcgt
       61 tgcccgattc ttcaccacaa acggaagctc gagcaccttt agctcgggaa cgatggccgt
      121 gaaacgcacg gtggacatgt agcagccggc aagctcgccg gtttcgacca tcgtcggcag
      181 ctcgcccgac ttgcggccga ggtcgagcac gttctccacg aggtcgaatg cgatagtgct
      241 gccgagcgta cgaatgagcc gctcgccgaa acgctgcgca gcccgattgt ggatggaggc
      301 ggttttctgg taaccaccga agcgaagctt gatgggctcc atgcgcggca ttctatccga
      361 tgcgcacgca ccagctgcgc cggccgctag gtggcctcca gcaggaactc gcgcacggcc
      421 gcgatctggt cggccgacat cagcatgggc gcatggccga cgccactgaa ctccgcgagc
      481 cgcgggcgcg gcccgcgcgc caccatcgcg gtggcaactt cgcgcggaag gagatcggaa
      541 tccgcgccgc gtacgagcag cgtcgggcag cgtatcgcat cccagtaccg ccacagatcg
      601 atatcgtgct gcggctgctc gtgaaacggc gccgcgatcg ccggatcgta ggccgtcgtc
      661 cagcttccgt cgtcgagctg ccgggccgca tgcacggtga gatgccgcca ctgcgcgtcg
      721 gtgagcgggc cgaagggtgc ggaaacgcgc cgcagatgcg cctccagcgc ctcgagcgtc
      781 tcgaagcggg gcgtgcgccc cagatacatt ccgatgcgtt cgagagccgc tttcggaata
      841 caggcgccga cgtcgttcac gacgagctta cggacgggcg agcgcggctg cgccgcgagc
      901 agcatgccga tgattccgcc catcgacgta ccgacccagt ggagttccca atgctccgca
      961 ccggcggtga gacgcgcgat cagcgcgttc atgtcggcca agtactgcgg gtagccgtaa
     1021 tcctcgctcg cgggaagcca gtcgctcaag ccgcgtccga cgatgtcggg gcacgccacg
     1081 cggaattccc cttgcagggc gcgggcgagg tcatcgaagt cgcgcccggt acgggtcagc
     1141 ccgtgcacgc agaccacgat gcgcggattg ccggcatcgc cccactccgt gtagtggaca
     1201 cgatggaaac cctttgcgct caatccgagg atgaaatcgt ttcgcatgca cggattctac
     1261 agcgcggctt cagaacatga agatcgccgc cgtaccggcg ccgatgaaga agacgaggtg
     1321 caccgtgctc cagaacgcga agcggcgccc caacctctca ggcagcgcgc acgcaagcag
     1381 gaagagccgc ccgtcgcgcg gcttgcgcat gaggtggacg ccctcggtaa cggctgcgta
     1441 accctctgcg tggcgcttgc ggacttcctt ccgggcttcg agacgcgcgt gctcccattc
     1501 cgggagatcg atttcgccgt ccttgttccg atcgaaacgc gcgagaagct gcgcccggtc
     1561 cttcttccag tcggcaagca ggtcgctcac gtccgcgcgc tcgtccagca gcgagattgc
     1621 cccacccgcc gtgctgaact cgcctatggc gtagagtctt cccttgggca cgatcagcca
     1681 ttcggtatgg cggtagttgc cctgcatcca cgtatccttg cgctcggtga ggatttcggc
     1741 gccttccgga gagatcacgc actcgccggt gtcgtctacc agcaggaaat gatcgttgct
     1801 ctcgccgctg tccacgcgtt cccacttgtc gttggaggtc ttgcgatcga tgcagtaacg
     1861 gcgccagcag cagggcagcc ccgttcggga actcaccagc gtgttgcccg ggagcagctc
     1921 ggaatggccg aaaagctcga cgtaaccctg cgccgcagac gcgaccttgg acgtcggatt
     1981 gtcgtggatg tgtcgatagc ggcggtaagt cgcggcccaa gcgaaaaagc ttgcgattgc
     2041 catggcggag agtgcccaag gccagacttc cgcggagtcg gctttgagcg cgatcagcag
     2101 aatgatgaag tgaatgacgc ccgtcagcca ctcggcgcgg tggtgggaga gggccatcgc
     2161 gcgaagaggt ttgcgggcgg atcaggcggc gaagagtttc tttacgtcga cgtccgcttt
     2221 ttccgtcgcg tcgaactcca gaagctgcgc gggcttgaat ccgaacatgc cggcgaggat
     2281 cgtatcgggg aactgctcga tgcgcacgtt attgatattg accgattcgt tgtagaactc
     2341 gcggcggtcg gcgatcgcgt tctcgaggct cgagatgcgc gcctgaagct gctggaacga
     2401 ctggttggtc ttgagatcgg gatacgcctc ggcgagcgcg aagaggttgc cgagcgaagc
     2461 gcgcagcatg ccctccgcct gcccgagcgc gccgatgttc tgggcttcgc gtgcggcgaa
     2521 cacgcgcgag cgcgcctcca tcacccgctc cagcgtttcc tgctcgaact tcatgtactg
     2581 cttgcacgtt tcgacgagct tgggcagctc atcgtggcgc tgcttgagca gcacgtcgat
     2641 gttggcccaa gccttcgaga cattgtgctt gatctggacg aggttgttgt aaatcatcac
     2701 gaagtacacg accagcacga gcagtgcgcc gagcacgatg acggtgccta ccatggttgc
     2761 cctcccccac agaatacgct gaacgattgt ataccggcat tgcggccgtt acgctttggc
     2821 gcccgtcgct tcggtggcgc cttccacttt ctcgaaatcg atgcgctcct gcaccggcag
     2881 gccctgcacg taccggcgca ggcaatcgag ccccatctct actgcaccga gacgcaccca
     2941 gtcgcgcccg cccacgatgc ggctgcggcg cgtcgcgatc tccgacgcgg tggcgatcgc
     3001 gatgcacacc gttccgccga tgtcgatgcg gtcgggacct tcgtcgattt cgatcaggac
     3061 cgccaatgca tgtgtggcgc ccgtctgcac gcgtatggca cgtgcgaccg cttcagcgct
     3121 ctcgcgcgtc acgccatcga tgcccggttg caggctgagg gcggcacaca gctgcgcgag
     3181 atcgcgggaa atgaggccgc ggcggatcac actttccgct cccggcagat gtccgatgcg
     3241 tgctgcaatc tgtccgctgg tgaaggtttc gacgagcgca agagtggcgc cgcggctcga
     3301 cagtgcctcg agcaccacct gctccagcgt ctgatcgcct tcggccagaa tgaagttgcc
     3361 gagccgccgg cgtacctccg cttcgaccgg agcgagcttg cgcttgatat cctccatgtc
     3421 cttgccgcgg acggtgagtt tcgtctcgag ttgcgggtaa tgcgcgcgaa agccgagctt
     3481 gacgctgccg tcgggcacca gcgcctcgac accggcgagc aacgcatcgg catgcgattc
     3541 cccgatgcca taggagtgga agcgcttcag gaagatcgcg gcctgcatgc cgctctttgc
     3601 gaggagtcgc ggaatgatct ctccctcaag catgcgacgc atttcgcgcg gcacgccggg
     3661 cgtgaaatag aagcgggcgc gtccgatatc gactgcgaag ccgcacgcgg tgccgaccgg
     3721 gttgtcgagg atttcggctc ccgcgggcag catcgcctgc ttgcggttgt tggccggcat
     3781 gacgcggctg cggcgccgga aatattcctc catgcgcgcg agccactcgt cgctcaggac
     3841 gagatccacg cctgccgcgc gggcggcaat ttcctgcgag aggtcgtcca ccgtggggcc
     3901 gagaccgccg ttcacgatca ccgcgtcggc gcgctcgcct gcgagccgga aggcgagaag
     3961 caggctctcg cgatcgtcgc cgaccgtggt ttcccagcgc accgaaaggc cgaagtcctc
     4021 gagcttctgg ctgatgtagc tgaagttggt gttgacgatc ttgccggtga gcacctcgtc
     4081 cccggtgcag atcacttcga tgcgcaattc gctgcccccg gtcatgtcgg cttgcggatc
     4141 aatcctcggt cgtgccgggc ttccgatcga taccgagcgg gcgtttcacc aactccggca
     4201 gcatggcgcg ttcgaagaat tggatgccga ctttctccag gcgtttttcg ccgatgagct
     4261 ccgcggcctt cgggcctgcc ttcttccatg ccttgtactc gtctgcgtat tggagcccga
     4321 tcgattgcgc cagcaggtga atgtagttat aggctttcgt gtcggcgtgc gcttcgagac
     4381 cgacgacttc ccgataacac tggtggaacg tcgtcaccac ggcgtccgcc ttgtggagtg
     4441 cgacgcggcg caacgtctcc cggtgcatgt cttcgagcgc tttcggcacg gaagcgagga
     4501 gtgcgcacat gtagcctggc gctgcgtagt cgtcgttcac cacttccaat ccgggaatga
     4561 gccgcagcag cgccgggact ttttcgttga ctttcacccg gtcgttgaag ccgacgtgct
     4621 tgtgcagcag cacgcgtatc ggaacgcgat gcttgaggag cgccgcgagc gcgtcccggc
     4681 gctcgtacag cagatcgacg agataggtga gtccgaaatc ggtcggatag gcccggccca
     4741 tgaactcggt catatgagca tgacacgaag gacaccaggc gatgaccttt tcccgggcca
     4801 tcgcattgaa tttcgtcacg gtgcgacgcg ccatgccgtc tgccgccgtc tgattgtcgt
     4861 ccttgccggt gccgcagcaa tagtcggggc cgcccgccgc ccgtgcgtcg atgcccagca
     4921 tcttcaggat atcgaggcag ccgtgaatga tgtcgccgtg ccgaaggacg ttgcagccgt
     4981 accagaatac gacccgctcg cccgcgtgcg tatcggaatt caggtccatt ccttgagctc
     5041 ttcttccgtc aactgcagtt tcgcgaaggc ccgcacccgg tcgttgaagc ccacgtgctt
     5101 gtgcagcagc acgc
//
LOCUS       DASURG010000179         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_542981, whole genome shotgun sequence.
ACCESSION   DASURG010000179 DASURG010000000
VERSION     DASURG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542981"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1309)
                     /gene="glcF"
                     /locus_tag="VFY80_03010"
     CDS             complement(44..1309)
                     /gene="glcF"
                     /locus_tag="VFY80_03010"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX6006902.1"
                     /translation="METHLAAFIRDTPEGREAEAILRRCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQMLEGAPVTARTRLHLDRCLTCRACETTCPSGVEYGRLADIGRDLV
                     ESRVPRRGLDYWKRRALRAVVPHRRRFGALLRLGQWVRPLLPRAVRQKVPERPHHASA
                     LQPPSSDEGKQRGKGTHPRKMLVLEGCVQPSLAPATNIAAARVLDRLGITLERSPEAG
                     CCGAVSFHLSAREEALRYMRRNIDAWWPAIEQGAEAVVMTASGCGTMVKDYGHALAHD
                     PVYAAKAQRVSELCRDVSEIIAAEEHRLAALLETARRPRARVAFHSPCTLQHGLRVRG
                     VIESILTRAGYTLVPVVDSHLCCGSAGTYSLLQSDLSQRLLENKVRSLTAGNPREILT
                     ANIGCQAHLQSGTALPVRHWIEALDEALS"
     gene            complement(1316..1813)
                     /locus_tag="VFY80_03015"
     CDS             complement(1316..1813)
                     /locus_tag="VFY80_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006903.1"
                     /translation="MANNFLEQLVAEWYEYKGYFVRRNVPVGRRARGGHESELDVVAF
                     NPTLRHLVHIEPSLDAESWDKRERLFRRKFEAGRKYIPDLFDGYELPPDIEQIAILVF
                     ASRSNHPTLGGGKVLLISDLMRQIMEDLGGKKPIANLVPEHHTILRTLQFVIEYRKKV
                     FDTLR"
     gene            complement(1820..>2422)
                     /gene="glcE"
                     /locus_tag="VFY80_03020"
     CDS             complement(1820..>2422)
                     /gene="glcE"
                     /locus_tag="VFY80_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX6006904.1"
                     /translation="ALGTLGVILEVSIKVLPLPVAEITVRREQTQADAIALVNEWAGK
                     PLPITATCHYGNALYVRLAGAAAAVRAAREKIGGEEVADGPAFWRAVRDQTLDFFQGL
                     EPLWRLSVKTTTPPLNLAGPQLIEWGGGLRWLCTDADAASVRHAAAFGGGHATLFRGG
                     DRAAGAFHPLTPALEQLHRRLKEKFDPAGIFNPGRLYPWM"
BASE COUNT          422 a          783 c          796 g          421 t
ORIGIN      
        1 cgcatggttt tcctccttgc agtgtcgatt cttcgcaatt gtctcaggac agcgcctcgt
       61 cgagcgcttc aatccagtgc ctgacaggta acgctgtgcc actctggaga tgcgcctgac
      121 agccgatgtt cgcggtcaga atctcgcgag gattccctgc cgtgagcgag cgcaccttgt
      181 tctcgagcag gcgctgcgac aagtcggact ggagcagcga ataggtgccg gcggacccgc
      241 agcacaaatg ggaatccacg acggggacga gcgtgtagcc ggcgcgcgtg agtatagatt
      301 cgattacgcc tctcacacgg agcccgtgct gcagcgtgca cggtgagtga aatgccacgc
      361 gagcgcgtgg acgccgagcg gtctcgagga gcgccgcgag ccgatgctcc tcggccgcga
      421 tgatctcgct cacgtcgcgg cacaattcag acacgcgctg cgccttcgcg gcataaacgg
      481 ggtcgtgcgc gagcgcatgc ccgtagtctt tgaccatcgt gccgcaaccg cttgcggtca
      541 tgacgacagc ctcggccccc tgttcgatcg ccggccacca cgcgtcgata ttccggcgca
      601 tgtatcgaag cgcctcctcg cgagcactca aatgaaatga gaccgccccg cagcagcccg
      661 cctccggcga gcgctcgagg gtgatgccaa ggcgatcgag aacgcgcgct gcggcgatgt
      721 tcgtggccgg tgcaaggctc ggttgcacgc agccctcgag caccagcatt tttctggggt
      781 gggtgccttt tccccgttgt ttaccttcat cggaagaagg cggctgcaaa gcgctggcgt
      841 ggtgcgggcg ctccggcacc ttttgccgga ctgcgcgcgg cagcagcggc ctgacccatt
      901 gtccgagccg cagcaacgcg ccgaaacggc gccgatgcgg aaccaccgcg cggagcgcgc
      961 gtcgcttcca gtaatccagg ccgcggcgcg ggacccgaga ctccacgaga tcgcgcccga
     1021 tatccgcgag ccgcccgtac tcgacaccgg aggggcaggt cgtctcgcac gcgcggcacg
     1081 tgaggcagcg gtccagatgg aggcgggtgc gcgcggtgac cggtgcgcct tccagcatct
     1141 gtttgatgag atagatgcgc ccgcgcggcc cgtccagctc atcacccagc agctggtagg
     1201 tggggcaggt cgcggtgcag aagccgcagt gcacgcagcg gcgcaggatc gcttcggctt
     1261 cgcggccctc gggcgtatcg cggatgaatg cggcgagatg cgtttccacg tcgcgttatc
     1321 gcagggtgtc gaacaccttc ttgcggtatt cgatcacgaa ttgcagcgtg cgcaggatcg
     1381 tgtggtgctc cggtacgaga ttggcgatcg gcttcttgcc gccgagatct tccatgatct
     1441 gacgcatgag atccgagatg aggagcacct tgccgccccc aagcgtcgga tggttgctgc
     1501 ggctcgcgaa gacgagaatt gcgatctgct cgatgtcggg cggcaactcg tagccgtcga
     1561 aaagatccgg gatatatttg cgccccgcct cgaatttccg gcgaaagagc cgctcacgct
     1621 tgtcccagct ttcagcgtcg agcgaaggct cgatgtggac gagatggcgc agcgtaggat
     1681 tgaacgcgac gacatcgagt tccgactcat ggccgccgcg cgcgcgccgc cccaccggca
     1741 cattgcggcg gacgaaatag cccttgtatt cgtaccactc tgcgaccagt tgttcgagga
     1801 agttattcgc catgatcggt cacatccatg gatagaggcg cccggggttg aagatgccgg
     1861 cgggatcgaa cttctccttg agccggcggt gaagttgctc gagcgcgggc gtgagcggat
     1921 gaaacgcacc cgccgcgcga tcgccgccac ggaaaagcgt ggcatgcccg ccgccgaacg
     1981 cggccgcgtg ccgcacggaa gcggcgtcgg catcggtaca caaccacctg agcccgccgc
     2041 cccactcgat cagttgcggc cctgccagat tgagcggcgg cgtggtggtc ttcacggaca
     2101 gacgccagag cggttcaagt ccctggaaga aatcgagggt ctggtcgcgc accgctcgcc
     2161 agaatgccgg gccgtcggcc acttcctcgc cgccgatctt ctcgcgggct gcgcgaaccg
     2221 cagcagcggc gcccgccagc cgcacgtaga gcgcatttcc ataatggcac gtcgccgtga
     2281 tcggaagcgg ctttcctgcc cactcgttca cgagtgcgat cgcgtcggcc tgcgtctgct
     2341 cgcgccgcac ggtgatctcc gcgaccggga gcgggagcac cttgatcgac acttcgagga
     2401 taacgcccag cgtgccgagc gc
//
LOCUS       DASURG010000180         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_952233, whole genome shotgun sequence.
ACCESSION   DASURG010000180 DASURG010000000
VERSION     DASURG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952233"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VFY80_03025"
     CDS             complement(<1..1171)
                     /locus_tag="VFY80_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6006905.1"
                     /translation="MEGIGLLYTGFVNSLTAVNLIACFVGVLVGTVVGVLPGLGPTAT
                     MVLMLPFTLPYGPTTGLIMMTGVWYGAMYGGSTTSVLVNIPGEAASVVTCIDGYQMSR
                     QGRAGAALALVAAGSFVAGTMGILGLQFFAPVLGNAALSFGPPEFLAFMILAFVLLSN
                     LSADAPLKGALMIAFGLFLSTIGINPMDSYPRFTFGIEKLMLGIDFLPIAVGLFGISE
                     VFRIAVEKYVKPAVSRVRLSELYPNRDEVKRSIAPMLRGSTLGFFVGLLPGPGTVIST
                     FVAYALEKRISRTPERFGRGAVEGVVAPEAANNSAVMGSMIPLLTLGIPFAAPSAIML
                     AGLRMHNVEPGPMLFVTHQEVFWTFVAAMYIGNLMLLLLNLPLVGMFARIATIRPQ"
     gene            complement(1181..1618)
                     /locus_tag="VFY80_03030"
     CDS             complement(1181..1618)
                     /locus_tag="VFY80_03030"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6006906.1"
                     /translation="MKLKSETIFIGVLLALGAAIAVVSWGYGFGTMQQPGPGLYPFFL
                     GAAIVIFSLVLMIDAKPAPAIADRPAAWTLALMTLTFCLWIVAMPLLGYVLVTLAAAY
                     AFCKIMKLEGWVKPLAVSVGTALFVYLLFDYWLYIDLPRGLLG"
     gene            complement(1621..2073)
                     /locus_tag="VFY80_03035"
     CDS             complement(1621..2073)
                     /locus_tag="VFY80_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX6006907.1"
                     /translation="MRGMYWEEWEVGVEFESPARTVTEADITLFAGLSGDYNPLHVNE
                     EYCKQTVFGGRIAHGPLVYAIAAGLLFQLHLYDDTLVAFLGFDSLKFMKPVKPGDTIH
                     AWIKVLEKRETSRADRGVMRRELRVINQRGEVVQEGVQTFLLKRKPAA"
BASE COUNT          475 a          685 c          686 g          355 t
ORIGIN      
        1 tctgcgggcg gatggtcgcg atgcgggcaa acattcccac cagcggcaga ttgagcaaca
       61 gtagcatgag gttcccgatg tacatggcgg cgacgaacgt ccagaagact tcctggtgcg
      121 tgacgaaaag catgggccct ggctcgacgt tgtgcatgcg caatcccgcc agcatgatcg
      181 cgctcggcgc cgcgaaggga atccccagcg tgaggagcgg tatcatcgaa cccatcacgg
      241 cgctgttgtt ggcggcctcc ggtgcgacga caccttccac cgcgccccgc ccgaagcgtt
      301 ccggggtgcg cgaaatgcgc ttctcaagcg cgtaggcgac aaaggtggaa atgaccgtgc
      361 ccggtccggg cagcagtcct acaaaaaatc cgagcgtcga gccgcgcagc atcggcgcga
      421 tcgagcgctt cacttcgtcg cggttcggat agagctcgct cagccgcacg cggctcaccg
      481 ccggctttac gtacttctcc acggcgatcc gaaaaacttc cgaaatgccg aagaggccca
      541 ccgcgatcgg caggaaatcg atgccgagca tcagcttctc gatgccgaaa gtgaagcgcg
      601 gatagctgtc catcggattg atgccgatcg tgctcaggaa gagcccgaac gcgatcatga
      661 gcgcgccttt gagcggcgcg tccgcagaga gattggaaag cagcacgaat gcgagaatca
      721 tgaacgccag aaactcgggc ggcccgaagg agagcgcggc attacccaac acgggtgcaa
      781 agaactgcag gccgagaatg cccatcgtgc cggcgacgaa agaccccgcg gcaacgagcg
      841 cgagcgccgc ccccgcgcgg ccctggcgcg acatctggta gccgtcgatg caggtcacca
      901 cgctcgcggc ttcacccgga atgttgacga gcaccgaagt ggtggagccg ccgtacatcg
      961 cgccgtacca cacgccggtc atcatgatga gtccggtggt cgggccatac ggcagggtga
     1021 atggcagcat gagtaccatg gtcgccgtag gtccgaggcc gggcagcaca ccgaccacgg
     1081 tccccacgag gaccccgacg aagcaggcga tcaggttgac ggcggtgagg gaattgacaa
     1141 acccggtgta gagcagcccg atcccctcca tttatcgcct ctacccgaga agtccgcgcg
     1201 gcagatcgat atagagccag tagtcgaaga gcaggtatac gaagagcgct gtgccaaccg
     1261 agacggcaag cggcttcacc cagccttcga gcttcatgat cttgcagaac gcatacgccg
     1321 cggcgagcgt gacgagcacg taacccagga gcggcatcgc cacgatccac aggcagaaag
     1381 tcagcgtcat gagcgcgagc gtccaggcag cagggcggtc ggcgatggcg ggcgcgggct
     1441 tcgcgtcgat catgagtacc agcgagaaaa tcacgatcgc ggcgccgagg aagaacggat
     1501 agagcccggg ccccggctgc tgcatggtgc cgaatccgta gccccacgag acgacggcga
     1561 tcgctgcgcc gagcgcgagc agcacaccga tgaaaattgt ctctgactta agcttcacgt
     1621 tcatgcagcg ggtttgcgct tcagcagaaa ggtctgaacg ccctcctgca cgacctctcc
     1681 gcgctggttg atgacgcgca gttcccgcct catgacgccg cgatccgcac gcgaagtttc
     1741 ccgtttctcc agcaccttga tccacgcgtg gatggtatcg ccgggcttga ccggcttcat
     1801 gaatttgagg ctgtcgaagc cgagaaacgc gacgagcgta tcgtcgtaga ggtgaagctg
     1861 gaagagcaat cccgctgcga tagcatagac aagcggcccg tgcgcgatgc gcccgccgaa
     1921 gaccgtctgc ttgcagtact cctcattgac gtgcagcgga ttgtagtcgc ccgaaagacc
     1981 ggcaaagagc gtgatgtccg cttcggtcac ggtgcgggcc gggctttcga actccacgcc
     2041 gacttcccat tcttcccagt acatcccgcg catcgtgccg tcctccgaca gtcgttgaaa
     2101 atcagctcgt cacaccggcg aaagccgatg tccggaaaac gtgcgtctat cagatccagc
     2161 cttgctggat gctggctatc gccggcatgg cgatatcgta a
//
LOCUS       DASURG010000181         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1155841, whole genome shotgun sequence.
ACCESSION   DASURG010000181 DASURG010000000
VERSION     DASURG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155841"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VFY80_03040"
     CDS             <1..1028
                     /locus_tag="VFY80_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6006908.1"
                     /translation="VNAVSLSIEEGELFTLLGPSGCGKTTLLRVIAGFNAPDEGVIRF
                     DARAVNAVPPHERGIGMVFQNYALWPHMTVGQNVAYGLKLRKVPTAEFAARVQSVLAK
                     VGLEGLDARYPGQLSGGQQQRVALARALVLNPQILLLDEPLSNLDAKIRVQVRAEIRK
                     LQKELGITTVYVTHDQEEALTLSDRIAVFNQGRLLQVGPPKALYERPTSRFVADFIGI
                     NNLLAGTVRSVEGPHDGVRIDTALGEIRAICGEALRVGDPCVICIRPENIGLAGATNG
                     LRNRFQGTISFAAYLGNTLRYDVDLGGGLVFKFDIGDPWHHEQMAAGTAVTLSCPVGS
                     TLAIPGD"
     gene            1033..>2727
                     /locus_tag="VFY80_03045"
     CDS             1033..>2727
                     /locus_tag="VFY80_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6006909.1"
                     /translation="MAAHTAAIRLQAPRISAGAIIGFALIWLFLLLFVVYPLTRIFYD
                     AFTNEAGQFTFVNFAEFFTDRFYLRSLWNSLVLGAAAVVTTSVIGIAVAFLIIRYEFP
                     GRNLFAYLTMLPLILPPLVGVLGFVFILGRAGTVNVFLMDTFDMLRPINFMYGWHGVL
                     LVETVHLFPLMTLSILDSLSKVDPSLEEAAQGMGAKGWRRFRDVTLPLMTPGYVSGAL
                     LVFIWTFADFVTPLVVGVQDLLAVQAYLNIVQFVDRRIFRMGIVISALLVLLAVIFVL
                     VARHYVAIKDYSSLAYSKVERRRLGTVKRWFAVGFLVLLLVISAIPQLGVVLAAVGKG
                     WALTPFPIHYTLDYFRQVSIETPKFFVNSLMFSGLAVLICLVVGVPMAWIMSRTRTIG
                     RGAMDALTTLILAIPGTAIGIAYIRAFHYPLPWIDIALTSTWFVLPLVLAVRRLPYTV
                     RGSYSSLLLVHKSMEEAAENVGATKLRTFRDITVPLVWKGILVGALFSFIMSIQEASA
                     TLFLTLGGWEMIPIGIFTYYIAGSHGQAAALGVILIALCAVSLFIVNRVAGARVGGL"
BASE COUNT          425 a          877 c          849 g          576 t
ORIGIN      
        1 ccgtaaacgc ggtgagccta tcgatcgagg aaggcgagct ctttacgctg ctcgggcctt
       61 ccggctgcgg caagacgacc ctgctcaggg tcatcgcagg cttcaatgcg ccggacgagg
      121 gcgtaatccg gttcgacgcg cgtgccgtca acgccgtgcc gccgcacgaa cgcggcatcg
      181 ggatggtatt tcagaactac gcgctgtggc cccacatgac cgttggccag aacgtcgcct
      241 acggtctcaa gttgcgcaag gttcccactg cagagttcgc cgcgcgcgtg caaagcgtgc
      301 tcgcgaaggt cgggctggag gggctcgatg cccgctatcc cgggcaactc tcgggcggtc
      361 agcagcagcg cgtggcgctc gcgcgggcgc tcgtgctcaa cccgcagatc ctgctgctcg
      421 acgagccgct gtcgaacctc gacgccaaga tcagggttca ggtgcgcgcg gaaattcgca
      481 agctccagaa agagctcgga atcacgaccg tgtacgtcac gcatgaccag gaggaggcgc
      541 tgaccctgtc tgaccgcatc gcggtattca accagggaag gctcctccag gtgggaccgc
      601 ccaaggcgct gtacgagcgg cccaccagcc gtttcgtcgc ggatttcatc ggcatcaaca
      661 atctcctcgc gggcaccgtg cgttcggtgg aaggtccgca tgacggggtc cgcattgaca
      721 cggcgctcgg tgagatccgc gcgatctgcg gcgaggcgct gcgcgtcgga gacccttgcg
      781 tgatttgcat ccgtcccgag aacatcggac tggccggagc aacgaatgga ttgagaaacc
      841 gcttccaggg gaccatttcg tttgcggctt acctcggcaa cacgctgcgc tacgacgtcg
      901 atctgggcgg ggggctcgtt ttcaagttcg acattggaga cccctggcat catgagcaaa
      961 tggccgcggg caccgctgtc acgctgagtt gcccggtggg cagcacgctc gcgattcctg
     1021 gggactaaag cgatggcggc gcacaccgca gcgatccggc ttcaggcgcc acgcatatcg
     1081 gcgggggcga tcatcggctt cgcgctcatc tggttgttcc tgctgctctt cgtggtgtat
     1141 ccgctgacgc gcatcttcta cgacgcgttc accaacgaag cgggccaatt caccttcgtg
     1201 aactttgccg aattcttcac ggatcgtttc tatctgcgct cgctgtggaa ttcgctcgtg
     1261 ctcggggccg cggcggtcgt cacgacctcg gtgatcggaa ttgcagtggc gtttctcatc
     1321 atccgctacg agtttccggg gcgcaatctc ttcgcctatc tcaccatgct gccgctcatc
     1381 ctgccgccgc tcgtcggtgt gctggggttt gtcttcatcc tgggacgggc cggcaccgtg
     1441 aacgtgtttc tcatggacac cttcgacatg ctgcgcccga tcaatttcat gtacggctgg
     1501 cacggcgtgc tgctggtcga gacggtgcat ctttttccgt tgatgacgtt gagcattctc
     1561 gattcgcttt ccaaggtcga tccgtctctc gaagaagcgg cgcagggcat gggcgcgaaa
     1621 ggctggcgac gctttcgcga cgttacgctg ccgctcatga cacccgggta tgtctccggc
     1681 gcgctgctgg tgttcatctg gaccttcgcc gacttcgtca cgccactggt ggtgggcgtc
     1741 caggatctgc ttgcggtgca ggcgtatctc aacatcgtgc agttcgtgga tcggcgcatc
     1801 ttccgcatgg gcattgtgat ctccgcgctg ctggtgctgc tcgccgtcat cttcgtgctc
     1861 gtcgcacgcc actatgtcgc gatcaaggac tacagctcgc tcgcctactc gaaggtcgaa
     1921 cggcggcggc ttgggaccgt caagcgctgg ttcgcggtgg gcttcctcgt gctgctactc
     1981 gtgatttccg ccattcccca actcggcgtc gtgctcgccg ctgtcggcaa aggctgggcg
     2041 ctcacgccgt tccccatcca ctacacgctc gactacttcc gccaggtgag catcgagacg
     2101 ccgaagttct tcgtgaactc cttgatgttc tcgggcctcg ccgtgctgat ctgcctcgtg
     2161 gtcggcgtgc cgatggcgtg gatcatgagc cgcacgcgca cgatcgggcg cggcgcgatg
     2221 gacgcgctga cgaccctgat actcgccatt cccggcaccg cgatcgggat cgcctacatt
     2281 cgggcattcc actacccgct gccgtggatc gacatcgcac tcacgagcac atggttcgtc
     2341 ctgccgctcg tgctggccgt gcggcggctg ccttacaccg tgcgcggcag ttattcctcg
     2401 ctgctcctcg tgcacaagtc gatggaggag gcggcggaga acgtgggcgc cacgaaactg
     2461 cgcactttcc gcgacatcac cgtaccgctg gtgtggaaag ggatcctggt cggggcgctc
     2521 ttctccttca tcatgtcgat tcaggaagcc tccgcaacgc tcttcctcac gctcggcggc
     2581 tgggaaatga tcccgatcgg gatcttcacc tactacattg cgggatccca cgggcaggcg
     2641 gccgcgctcg gcgtcatttt gatcgccctc tgcgcggtga gccttttcat cgtgaaccgc
     2701 gtcgccggcg cgcgcgtcgg cgggctc
//
LOCUS       DASURG010000182         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_441143, whole genome shotgun sequence.
ACCESSION   DASURG010000182 DASURG010000000
VERSION     DASURG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441143"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1079)
                     /locus_tag="VFY80_03050"
     CDS             complement(111..1079)
                     /locus_tag="VFY80_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006910.1"
                     /translation="MGRILTVLLASLFAAGASAADPAASYPNRPVRLITGSPGSTSDI
                     AARFVAQKLAERWGQQVVVDNRPGAGGIIGAEIAARAAPDGYTLMVGQIGTHASAQFL
                     FKKLAYDPVKDFLPITLMTNSGIALAVNPSVPAKDLKSFVEYAKAKPGSVNYGSAGGG
                     TSSQLSGELFNQMTGARLVHIPYKGAGPALTALIAGETQAAFLSTTTISAQAKAGKVR
                     PLAVLSEKRFPAAPDIPSAGEAGFAGLDSSVWFGLFAPAKTPKPIVVKVNRDVVEILR
                     SPDAKAALLKQGAEAVPTTPEEFGAFLKKEIAKWSKVIKEAGIKAN"
     gene            complement(1091..2173)
                     /locus_tag="VFY80_03055"
     CDS             complement(1091..2173)
                     /locus_tag="VFY80_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6006911.1"
                     /translation="MTVAAAKDKRVEIAIHHWAPRFVSSGIPLADFEEVTASITRWEE
                     WCDAWSRRADVHEGLGRQALAAGCRYSAGQHLTRAAMCYHFGKFMFVHDIERMRRAHR
                     KVIECRDLALPHLDPPGERVAIAYSGKKLYGILRKPYGDLRPPVVVMCVGLDSTKEEL
                     DVYENIFLARGMATLAFDGPGQGEAEYDLPIRGDFEVPVKAVFDFLEARSDLDAKRAG
                     IWGVSLGGYYAARAAAFEKRAKACISLSGPYSWVETFDARNELSREAFRVRSHSKSME
                     TAREVAKTLTLEGVAKNITCPIFVVGGELDNLTPPHNAKRIAAEVSGPCELLIVKGGN
                     HVANNRRYMFQTQTADWMGRHLGIAQ"
     gene            complement(2192..3289)
                     /locus_tag="VFY80_03060"
     CDS             complement(2192..3289)
                     /locus_tag="VFY80_03060"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6006912.1"
                     /translation="MAQSDSSSAARDARVEAAIHHWAPRFVANGVPLADFQEVTAGLS
                     RWEAWCEAWSARGAVHESLGEQALGAGYGLTAGAHFNRAALCYHFAKFLFVNDLEQMR
                     AAHLKAVACRTRALPLSDPPGVRVTIAHEGAQLYGILRKPARSAMPPIVVMCMGLDSA
                     KEEMASNENVFLERGMATLTFDGPGQGEAEYALPIRGDYESAVKSVVDWVERRDDVDG
                     SRIGLWGVSLGGYYAPRAAAFEKRIKACIALSGPYDWAEIWPQLPELTREAFRARAKC
                     ATQKEARAVGATLTLKGVAEKITCPLFIVAGKLDRLVPWQHGERLARETGGEVEFLLI
                     EDGNHVANNRAYKYRTQSADWMARRLGCPAA"
     gene            complement(3418..>4191)
                     /locus_tag="VFY80_03065"
     CDS             complement(3418..>4191)
                     /locus_tag="VFY80_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6006913.1"
                     /translation="RSSAKQLEALKAGDFDIGFQQCDHVVRAVEQGSDLFMFMALAHA
                     PDLSLVVAPDVRAFHDLSGKIIAVDGARSGYALLLRRLFAEKGLKDGDYTFSEIGGSR
                     ERHDAVRDGRAAASLLNPPFDRNLLAAGFRSLGTTAQYFPKYTGSIAAARRSWARANE
                     TALVAFIRGMNAAYDWLRDPAHKSNAIEIVRARLDLSRDTASEAFDEIAKRPRPEIRT
                     EGLRQVIDVVWEAEGYVSPKGDPARYMDLSYYDRATRAR"
BASE COUNT          718 a         1406 c         1310 g          757 t
ORIGIN      
        1 atcactgtcg tcttgccggt gaaaaccggc atccggttct tttcctcgat tcgggccccc
       61 gtttgcaacc tgcggggcat gtcttcagac gccggaatga aggaccgtcg tcaattcgcc
      121 ttgatgcccg cttccttgat gaccttgctc cacttcgcaa tttccttttt caggaatgcg
      181 ccgaattcct ccggtgtcgt cggaacggct tccgcgccct gcttcagcag cgccgccttt
      241 gcgtccggcg atcgaaggat ctcgacgaca tcgcggttga ccttcacgac gataggcttc
      301 ggcgtctttg ccggcgcaaa caatccgaac catacgctgg agtcgagacc ggcaaagccg
      361 gcctctcccg cgctcgggat gtcgggcgcg gcggggaatc gcttctcgct cagcaccgcg
      421 agcgggcgca ccttgccggc tttcgcctgc gccgaaatcg tggtggtgga caggaacgcc
      481 gcctgtgttt cccccgcgat gagcgccgtc agtgcggggc cggcaccctt gtacggaatg
      541 tgcaccagcc gcgcgcccgt catctggttg aacagctcgc ccgagagctg gctgctcgtg
      601 ccgccgcccg ccgagccgta gttcacggag ccaggcttcg cttttgcata ttcgacgaag
      661 ctcttcaggt ccttcgcggg caccgagggg ttcaccgcaa gcgcgatgcc cgaattggtc
      721 atgagcgtaa tcggcagaaa atccttcacc ggatcgtagg cgagtttttt gaagaggaac
      781 tgcgcactcg cgtgcgtgcc gatctggccc accatcagcg tatagccatc gggcgccgca
      841 cgggccgcga tttccgctcc gatgatgccg cccgcgccgg gacggttatc gacgacgacc
      901 tgctggcccc agcgctccgc gagtttctgc gcgacaaaac gcgcggcgat gtccgaggtg
      961 ctgcccggcg agccggtaat gaggcgcacc gggcggttgg gatagctcgc agcgggatcc
     1021 gcagccgatg cgccggccgc gaatagagac gcgagaagaa cggtaagtat gcggcccatg
     1081 tggaattccc ctattgtgcg atgccgaggt gccgacccat ccagtcggcg gtctgcgtct
     1141 ggaacatgta gcggcggttg ttggcgacgt ggttcccgcc tttcacgatc aacagctcgc
     1201 acgggcccga aacctccgcg gcgatgcgct tcgcgttgtg cggcggcgtg agattgtcga
     1261 gctcgccgcc aactacgaat atcgggcacg taatgttctt cgccacgcct tccagcgtga
     1321 gcgtcttggc gacttcgcgt gccgtctcca tcgatttcga atgcgagcgc acgcgaaagg
     1381 cctcgcgcga aagctcgttc cgcgcgtcga atgtctccac ccagctgtac ggacccgaca
     1441 gcgaaatgca cgccttcgcc cgcttctcga acgcagcggc gcgcgcggca tagtagccgc
     1501 cgagcgaaac gccccagata ccggcgcgct tcgcatcgag gtcgctgcgc gcctcgagaa
     1561 agtcgaagac cgctttcacc ggcacctcga aatcgccgcg gatcggtaga tcgtactccg
     1621 cctcgccctg ccccggcccg tcgaatgcga gcgtcgccat gccgcgtgcg agaaagatgt
     1681 tttcgtacac gtccagctct tccttggtgg aatccagccc gacgcacatg acgacgaccg
     1741 gcggcctcag gtcaccgtat ggcttgcgca ggatgccgta gagcttctta ccggagtagg
     1801 caattgcgac gcgctcgccc ggaggatcga gatgcggcag cgcaagatcg cggcattcga
     1861 tgaccttgcg atgggcgcgc cgcatgcgct ctatgtcgtg gacgaacatg aacttgccga
     1921 agtgataaca catcgccgca cgcgtgagat gctggccggc cgagtaacgg cagcccgcgg
     1981 caagcgcctg ccggcccaat ccctcgtgca cgtcggcgcg gcgcgaccag gcatcgcacc
     2041 attcttccca gcgggtgatc gaggcggtta cttcctcgaa atccgcgagc ggtatgccgc
     2101 tcgagacgaa ccgcggcgcc cagtggtgga tcgcgatctc gacccgcttg tcctttgctg
     2161 ctgcaaccgt catgtagggg tccttacgaa ttcatgccgc cgggcatccc agtcggcgcg
     2221 ccatccagtc ggccgactgc gtgcggtact tgtaggcccg gttgttggcg acatgattgc
     2281 cgtcctcgat gagcaaaaac tccacttcgc cgcccgtctc ccgcgcgaga cgctcgccgt
     2341 gctgccacgg cacgaggcgg tcgagcttgc cggcgacgat gaaaagcgga caggtgattt
     2401 tctccgcgac gcccttgagc gtcagggttg caccgaccgc gcgcgcctcc ttctgcgtcg
     2461 cgcacttcgc ccgcgcgcgg aacgcttcgc gcgtcagctc cggcagctgc ggccagatct
     2521 ccgcccagtc gtacggcccg gaaagggcga tgcaggcttt gatgcgcttc tcgaatgccg
     2581 cggcgcgcgg cgcgtagtaa ccgccgagcg agacacccca gaggccgatg cgcgagccgt
     2641 cgacgtcatc acgccgttcg acccagtcca ccaccgactt cacggcggat tcgtagtcgc
     2701 cgcgaatcgg aagcgcgtac tccgcctcgc cctgtcccgg gccatcgaag gtaagggtcg
     2761 ccatgccgcg ctcgaggaaa acgttctcgt ttgaggccat ctcctccttc gccgaatcga
     2821 gtcccatgca catgacgacg atcggcggca tcgccgagcg cgccggcttg cgcaggattc
     2881 cgtagagctg cgcgccttcg tgggcgatcg tcacccgtac tccgggcgga tcgctgagcg
     2941 gcagcgcgcg cgtgcggcac gccacggctt tcagatgcgc cgcacgcatc tgctcgagat
     3001 cgttcacgaa aaggaacttg gcgaagtgat agcacagcgc cgctcgattg aagtgggcgc
     3061 cagccgtgag cccgtagccg gcgccgagcg cctgctcgcc cagtgactcg tggactgcgc
     3121 cgcgcgcgga ccacgcttca caccacgctt cccatcgcga caggcccgcg gtcacttcct
     3181 ggaaatcggc gagcggcacg ccgtttgcca cgaaccgcgg cgcccagtgg tggatcgccg
     3241 cttcgacgcg tgcgtcacgc gcggcagacg aggaatcgga ttgggccatg atcgtacgaa
     3301 ctgtgtgggg aaccgcggcg accgcggaac aggggcttcg cgtcggtatc ggaatgatac
     3361 agcgcctctg cccccgccgt ggaatcaaga tgcatgatgc cctgtgtgcg cgaggtgtca
     3421 gcgcgcgcgg gtcgcccggt cgtaatagga gagatccatg tagcgtgcgg gatcaccctt
     3481 gggcgacacg tatccctctg cctcccatac gacgtcgatg acctgacgca agccttctgt
     3541 ccggatttcc ggtcttgggc gcttcgcgat ctcgtcgaac gcctcggacg ccgtgtcccg
     3601 agacagatcg agccgggcgc gcacgatttc gatcgcgttc gatttgtgag ccgggtcgcg
     3661 caaccagtca tacgcggcgt tcatgccgcg tatgaatgcg acgagcgctg tttcgttcgc
     3721 gcgcgcccag ctgcggcgtg cggccgctat cgagcccgta tacttcggga aatactgcgc
     3781 tgtcgtgccc aggctgcgga agccagccgc taaaagattg cgatcgaacg gcgggttgag
     3841 caggctagcc gcggcacggc catcgcgcac cgcatcgtgc cgctcccgcg aaccaccgat
     3901 ctcggaaaaa gtatagtcac cgtcctttaa gcctttttcg gcgaacaggc gccgcaacaa
     3961 cagcgcgtag cccgagcgtg cgccatccac cgcgatgatc ttgccgctga gatcatggaa
     4021 agcgcgcaca tccggcgcga ccacgagcga cagatccggt gcatgcgcca gcgccatgaa
     4081 catgaagaga tcgctcccct gctcgaccgc acgcaccacg tggtcgcact gctggaagcc
     4141 gatgtcgaaa tcgcccgcct tcagcgcctc gagctgcttc gctgacgatc g
//
LOCUS       DASURG010000183         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_33109, whole genome shotgun sequence.
ACCESSION   DASURG010000183 DASURG010000000
VERSION     DASURG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33109"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFY80_03070"
     CDS             complement(<1..713)
                     /locus_tag="VFY80_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006914.1"
                     /translation="MKKLSGPSRFAVAVIALFAMSATAALAAEKGGSYPQRPVRIVVP
                     VSAGGGVDSLARLIAQHYNATWGQPFIVDNRTGAGGAIGAEIAARAAPDGYTLLVSSS
                     SLVTNAAIRDVRYDPVRDFQPVSKLTANPYIVLVTPALGVSSVKQLIALAKSQPGTIT
                     YASSGTGGVLHLGAELLCALSGVQMTHVPYKGVADGYPAVISGEVKWMLGSPISALPL
                     MKAGRLKGLAVTSAHRSKA"
     gene            983..2014
                     /locus_tag="VFY80_03075"
     CDS             983..2014
                     /locus_tag="VFY80_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6006915.1"
                     /translation="MKAAVLHGASDLRFETVPDPGPARDEVIVKVQACSICGSDLHGF
                     HGKHPRLVFPRILGHEFAGEVVALGAGVKGVAPGTRVCCDIDIYCGQCGPCREGRTNI
                     CEKLRTMGFDRDGAYAEYVAVPAINLYPLPENVSYDQAAAVQVLGISYHAVAHRVVPR
                     RGEQVAVIGGGPIGLGAALVAKSLGAEVTLFELLDYRLAMARKLGIERVLDPSNIDVR
                     GEALALTGGRGFDKVVECVGGFQEKTVQMAVDMVKRGGQITVVGTFPENRATIPIAYI
                     KDREIDLNFSRGNFQAFAPCLELIARGAVNPDLYISHRIPLTRAVDALRLLEARDVEA
                     HKIVLHPQA"
     gene            2038..>2498
                     /locus_tag="VFY80_03080"
     CDS             2038..>2498
                     /locus_tag="VFY80_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006916.1"
                     /translation="MNRVRAGILATGAIFCALAAAGAPAAEAQAKYPMRPIRILIPFP
                     AAGASDTIGRTIGEQLSAQLGQPVVIDNRPGAAGRLATEMLARAEPDGYTLLVGGVGP
                     LAISPGLYSKLPYDSARDFAAITRVAEIINVMVLNASSGVTSPAQFIEWAK"
BASE COUNT          426 a          815 c          826 g          431 t
ORIGIN      
        1 aacgccttgc tgcgatgggc ggaggtcacc gcgaggcctt tgagacgccc cgccttcatg
       61 agcggcagtg ccgagatcgg gcttccgagc atccatttca cctcgcctga aatcacggcg
      121 ggatagccgt cggcgacgcc cttgtagggc acatgcgtca tctgcacgcc gctcaacgca
      181 caaagcagct cggcgccgag gtgcagcacg ccgcctgttc ccgaagaagc gtaggtgatc
      241 gtgccgggct gcgatttggc gagcgcgatg agctgcttga ccgaggacac cccgagcgct
      301 ggggtgacga gcacgatgta ggggttggcg gtgagcttgc tcaccggctg gaagtcgcgc
      361 accggatcgt aacgcacgtc acgaatggcg gcattcgtca cgagactgct gctgctcacg
      421 agcagggtat agccgtcggg cgccgcccgg gccgcgatct cggcgcctat cgcgccaccc
      481 gcgccggtgc gattgtccac gatgaagggc tggccccagg tcgcgttgta gtgctgtgcg
      541 atgagtcgcg ccaggctgtc caccccgccg cccgcgctca ccggcaccac gatgcggacc
      601 ggtcgctgcg gatatgagcc gcctttctct gcagcgaggg ccgcggtcgc gctcattgcg
      661 aaaagcgcga taacggccac cgcaaaccga ctcggtcccg aaagcttctt cattggatct
      721 cctcgattgc atgctgcggg tcacgtgcac tatcgcacga accgcaggcg atttaccgac
      781 cttttgtgaa cggcatcacg gacgccgctg caacagcgat tcatgatacg cgcacagcac
      841 catacttcac tcctcctgtg ggtgttgtga ctgccgcgca cggactctcc ggcgcggctt
      901 tttattttcc ctcgcgccac tgcgctgcac gcttccgcta tcataccgcc ctttatcctg
      961 cgatctgagc gaaggaggcg aagtgaaggc ggcggtcctg catggggcga gcgatctgcg
     1021 tttcgagaca gtgccggacc ccggtccagc gcgcgatgaa gtgatcgtaa aggtgcaggc
     1081 ctgttcgatc tgcggatccg atcttcacgg ctttcacggc aagcatcccc ggctcgtgtt
     1141 tccccgcatc ctgggccacg agttcgcggg cgaggtggtc gcgctcgggg ccggtgtgaa
     1201 gggcgtcgct cccggaacgc gggtgtgctg cgacatcgac atctattgcg ggcagtgcgg
     1261 gccgtgccgc gagggccgga ccaacatctg cgaaaagtta cgcaccatgg gtttcgatcg
     1321 cgacggcgcc tacgccgaat acgtcgcggt tccggccatc aacctgtacc cgttgccgga
     1381 gaacgtaagc tacgaccagg ctgcggcggt gcaggtgctc ggtatcagct atcatgccgt
     1441 cgcgcaccgc gtggtcccgc gtcgcggcga gcaggtggcc gtgatcggcg gcggacccat
     1501 cgggctcggg gcggcgctgg tggcgaaatc gctcggcgcc gaagtgacgc tcttcgagct
     1561 gctcgattac cgcctcgcca tggcgcgcaa gctcggcatc gagcgtgtcc tcgatccctc
     1621 gaacatcgat gtgcggggcg aagctctggc gctcaccggc gggcgcgggt tcgacaaggt
     1681 ggtggagtgc gtcggcggat tccaggaaaa gaccgtccag atggcggtcg atatggtgaa
     1741 gcgcggcggc cagatcaccg tcgtcggcac ctttccggag aaccgcgcca ccatcccgat
     1801 cgcctacatc aaggatcggg agatcgacct caatttctcg cgcggcaatt tccaggcgtt
     1861 tgcgccgtgc ctcgagctga tcgcgcgggg cgccgtcaat cccgacctct acatcagcca
     1921 ccgcattccg cttacccggg cggtggacgc actgcgcctg ctggaggcgc gcgacgtgga
     1981 agcgcacaag atcgtcctgc atccgcaggc atgaccccac aacgaggagg aacagcaatg
     2041 aatcgagtac gggcgggaat tctcgcgacc ggcgcaatct tctgcgcgct ggcagcggcg
     2101 ggcgcgccag cggcagaggc gcaggcgaag tatccgatgc ggccgatacg catccttata
     2161 ccgtttcccg cggcgggcgc gtccgacacg atcggccgca ccatcgggga gcagctctcc
     2221 gcgcagctcg gccagcccgt cgtgatcgac aatcgccccg gcgcggcggg ccggctcgcg
     2281 accgagatgc tggcgcgcgc ggaaccggac ggatacacgc tgcttgtcgg cggcgtcggg
     2341 ccgcttgcca tcagccccgg gctctacagc aagctgccct atgacagcgc gcgggatttc
     2401 gcggcgatca cgcgcgtggc ggaaatcatc aacgtcatgg tgctgaacgc atcgagcggc
     2461 gtaacgagtc ccgcccagtt catcgagtgg gcgaaaaa
//
LOCUS       DASURG010000184         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_952952, whole genome shotgun sequence.
ACCESSION   DASURG010000184 DASURG010000000
VERSION     DASURG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952952"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFY80_03085"
     CDS             complement(<1..713)
                     /locus_tag="VFY80_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006917.1"
                     /translation="MNRNVRSYSTVCALALAAASLLAGALPAWSQQYPTKPVRVIVPF
                     APGGGSDITARQVSAKFSEAFKQQFVVENRGGAAGLIGMEMTAKAAPDGYTIMIMSGS
                     FSATSATHQPAFDPINNIVAVAEVGFTPFVLSVHPSLPSKTTKEFIAMVRAKPGELVY
                     ASSGVGGLTHMATELLASMGKLKMIHVPYKSTGAAMADLLSGRAQLIVGSLLPTVPHF
                     KTGKLRPLSVTTAKRWYSLP"
     gene            complement(785..1774)
                     /locus_tag="VFY80_03090"
     CDS             complement(785..1774)
                     /locus_tag="VFY80_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6006918.1"
                     /translation="MANIDLHNHVVPPTIVDAIARNPERFGTRFVEKEGKRYFDAHGR
                     LAQLFPEFYDVDAKVAWMDRKGIDIAAISVGPPIYFYWLSAEAGLAAAKLANDGIAQM
                     VAKRPDRLRGMAHLPMQDPDAAITELERVVREHKFRAVEMGTSIEGIALADRRFRKVL
                     KTIEQLGCFIFAHPYQCLARGAMDDYYLSNFVGFPLDTTLMIAHLMFSGALDELKKLR
                     ILCAHGGGFMPYQIGRFAHGHRVRPEPKVNNPSPPRELFRRFYFDCLTHDPLAARHLI
                     NIVGPDRVAIGTDNPFDMQPDDPMGAIDAIPDLSASEREFVCELTARHLLGED"
     gene            complement(2348..2512)
                     /locus_tag="VFY80_03095"
     CDS             complement(2348..2512)
                     /locus_tag="VFY80_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006919.1"
                     /translation="MKRTTHRSVAGTKLYAVRDSKGRFKDIQTYKRAHAQDLKRRSKA
                     EAPMKQPVES"
     gene            complement(2560..>2812)
                     /locus_tag="VFY80_03100"
     CDS             complement(2560..>2812)
                     /locus_tag="VFY80_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX6006920.1"
                     /translation="LKNTLARIPNVLVKPAADVEILSFNERGPVLAVRPYCNNEHYWQ
                     VYFDTNRVIRETFSAAGYPVPEAHQVMRTMAEKAMPRGA"
BASE COUNT          507 a          857 c          890 g          558 t
ORIGIN      
        1 ggaagcgaat accaccgttt cgcagtcgtg accgagagcg gccgcagctt gccggtcttg
       61 aagtgcggga cggtaggcag cagactgccc acgatgagct gcgcgcggcc cgacaggaga
      121 tcggccatcg ccgcaccggt gctcttgtat ggcacgtgaa tcatcttgag cttccccatg
      181 ctcgcaagaa gctccgtcgc catatgcgtc aacccgccga cgccgctcga ggcatacacg
      241 agctcgcccg gcttggcgcg aaccatggcg atgaattcct tggtcgtctt cgaggggagc
      301 gagggatgca ccgagagcac gaacggcgtg aatcccactt cggcgacggc gacgatattg
      361 ttgatcgggt cgaacgccgg ctggtgcgtg gcggaagtgg cggaaaagct gcccgacatg
      421 atcatgatcg tgtagccgtc cggcgccgct ttggcggtca tttccatgcc gatcagcccg
      481 gctgcaccgc cgcggttctc gacaacgaac tgctgcttga acgcctcact gaatttcgcg
      541 gacacctggc gcgccgtaat gtcgctgccg ccgcccggcg caaacggcac gatgacgcgc
      601 accggtttag tcgggtattg ctggctccat gccggcagcg cgccggcgag gagggacgcc
      661 gcagcgagcg caagcgcgca tacggtggaa tacgacctca cattgcggtt catgatctct
      721 ccttgtgtga atgtacgggg tgtgtcggta agtcgataca gcgatcaacg gtgaaaaaat
      781 gcgatcaatc ctcgccaagc agatggcgtg cggtcaactc gcacacgaac tcgcgctcgc
      841 tcgccgacaa atccgggatc gcgtcgatcg cccccatcgg gtcatcgggc tgcatgtcga
      901 acggattgtc agtgccgatt gccacccggt ccggccccac gatattgatg agatggcgcg
      961 cggccagcgg atcgtgcgta aggcaatcga agtaaaagcg ccggaaaagc tctcgcggtg
     1021 gagaagggtt gttgaccttg ggctcgggcc tcaccctgtg gccgtgggcg aagcgcccga
     1081 tctggtaggg catgaatccg ccgccgtgtg cgcagagaat gcgcagcttc ttcagttcat
     1141 cgagcgcacc gctgaacatc agatgcgcga tcatgagcgt cgtgtcgagc gggaagccga
     1201 cgaagttgct caggtagtaa tcgtccatgg cgccccgcgc aagacactgg tagggatggg
     1261 cgaagatgaa gcagccgagc tgttcgatcg ttttcagcac cttgcggaac cgcctgtccg
     1321 cgagcgcgat accttcgatc gacgtgccca tctccaccgc cctgaatttg tgctccctca
     1381 cgacccgttc gagctcggtg attgccgcgt cgggatcctg catcggaaga tgcgccatgc
     1441 cgcgcaggcg gtccgggcgc ttcgcaacca tctgcgcgat gccgtcattg gcaagcttcg
     1501 ccgccgcgag ccccgcctcc gcggacagcc agtagaagta gatcggcgga ccgacggaga
     1561 tggcggcgat atcgatgcct ttgcggtcca tccatgcgac cttcgcgtcg acgtcgtaga
     1621 actcggggaa cagctgagcc aggcggccgt gcgcgtcgaa atagcgcttg ccctccttct
     1681 ccacgaaacg ggtgccgaag cgctcgggat tgcgcgcgat cgcatcgaca atggtcggcg
     1741 gaacgacgtg gttgtggaga tcgatgttcg ccatgattcc ggtatgctcc tccagtgcgt
     1801 tttgagggcg agcacaacgc ggcaaccggg tacggcgctt ggcgggagtg cgcgcgacag
     1861 cgtggctggc attctagttg catgctcgtc atgtcgccac tccccggggc cgcggagcgc
     1921 gtgccccggc aggagcatgc ggtatgctgc gcggcatttc ttcggagatc cagcatcctg
     1981 cccggaagtg atgcctactt tgacgtagcg caaagtcatc ggtcgtgatt cgggttagat
     2041 tccaggcgcg ctctcgcgtt gacgcgcttt cttccgatac ttgcaggaga atcacatgac
     2101 taagcacgcc ggtcatgcac ggtcgatcct cgatcgttcc ttccgttatg ttccgagcgt
     2161 cgaaaccgac ctaagaaaga ctttcgcgag ggtgcgccgt caacagcggc tgcaggcccg
     2221 cgcccagtcg gcaggtgatg tgcctcgggg gaaagtggcg ccgttttcgg tttctttgcg
     2281 cgcggtcacc aaacgataac ccgccccgcg ttctaccccg cgcgcgggcg caggacacaa
     2341 acccgcttta gctttccacc ggctgcttca ttggggcttc cgccttgctg cggcgcttga
     2401 gatcctgagc gtgcgcgcgc ttataggtct ggatgtcctt gaagcgacct tttgaatcgc
     2461 gaactgcgta gagcttggtg cctgcgacac tgcgatgcgt cgttcgtttc atgactcgac
     2521 cccttcgtga atgcaaatgc tttcgttccg gacccgcagc taggctcccc gcggcattgc
     2581 cttttctgcc atggtgcgca tcacctggtg tgcttcgggc acggggtagc ccgcggccga
     2641 gaatgtttcc cggatcacgc ggttggtgtc gaagtacacc tgccagtaat gctcgttgtt
     2701 gcagtatggg cgcaccgcga gcaccggccc gcgctcattg aacgacagga tttcgacgtc
     2761 cgcagccggc ttcaccaaca cgttgggaat tcgagcgaga gtattcttca gg
//
LOCUS       DASURG010000185         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_747893, whole genome shotgun sequence.
ACCESSION   DASURG010000185 DASURG010000000
VERSION     DASURG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747893"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..2073
                     /gene="secD"
                     /locus_tag="VFY80_03105"
     CDS             196..2073
                     /gene="secD"
                     /locus_tag="VFY80_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6006921.1"
                     /translation="MNRYPVWVYAIVAIALVVGFVYTLPNFFGEAPAVQVSPVKATLK
                     ADAALLERVDAILKENGIPHDGITVDESGVKARFVEPETQIKAKDVLQAKLGDDYVVA
                     LNLVSNSPRWLSAIGALPMYLGLDLRGGVHFLMQVDMKTALTRKTEGIAGDIRGALRD
                     KRIPYAGISREGDRVVVRFRDQAAREKAIAEIERMTGDLNLRLVDDAGDFRLVATLKP
                     EAQKKTADFALQQNITTLRNRVNELGVAEPIIQQQGADRVVVQLPGVQDTAKAKEILG
                     RTATLEVRMVDEENSNNPAVLEAATRGQVPFNSELFYERTERGSVPILVRKQVVLTGE
                     RINDAQPGFDNQTGEPAVHISLDGAGARIFQQVTRENVGKRMAIILFEKGKGEVVTAP
                     VIRTEIGGGRVQISGRMNTREANDVALLLRAGALAAPMEIIEERTVGPSLGAENIEKG
                     FRATWYGAAAIAAFMITYYALFGVISVLALAANLMFLVALLSMLQATLTLPGIAAIAL
                     TLGMAIDANVLINERVREELRNGMSPQAAIHAGYERAWATILDSNITTLIAGIALFAF
                     GSGPVRGFAVVHCLGILTSMFSAVVVSRSIVNLYYGRKRRLDGVSIGQVWKPGAEAPA
                     R"
BASE COUNT          401 a          695 c          741 g          350 t
ORIGIN      
        1 tttcgaagat cggcgacaac tacgtctcgc tgcaggtcgc cgacaacgtc gagatcatgg
       61 tgcagcgccc ttccgtccag ctcgtcctgc ccaagggaac gctgaagacg ctgtgaacgg
      121 cgcgccgccc gaggttcgat ccgtgagcgt tgtccactcc tgcgacgggg ccgcagccat
      181 ttccgtttac cagaaatgaa tcgatatccg gtttgggtgt acgcgatcgt cgcgatcgca
      241 ctcgtggtcg gcttcgtcta cacgctgccg aacttcttcg gcgaagcgcc ggcggtccag
      301 gtctcgcccg tgaaggcgac gctgaaggcg gacgccgcgc tgctcgagcg cgtcgacgcg
      361 atcctgaagg agaacgggat cccgcacgac ggcatcacgg tcgacgaaag cggcgtcaag
      421 gcgcgcttcg tcgagccgga aacccagatc aaggcgaagg acgtgctgca ggcgaagctg
      481 ggcgacgact acgtggtcgc tctgaatctg gtctcgaaca gcccccgctg gctgagcgcg
      541 atcggcgcac tgccgatgta tctgggcctc gacttgcgcg gcggcgtgca cttcctgatg
      601 caggtcgaca tgaagaccgc ccttacgcgc aagaccgagg gcattgccgg cgatatccgc
      661 ggcgcgttgc gtgacaagcg cattccttat gcgggcattt cgcgcgaagg cgatcgcgtc
      721 gtggttcgct tccgcgacca ggcggcgcgg gagaaggcca ttgccgagat cgaacgcatg
      781 acgggcgatc tcaacctgcg cctggtggac gatgccggcg atttccggct cgttgccacg
      841 ctcaagcccg aggcgcagaa gaagaccgcc gatttcgccc tgcagcagaa catcacgacg
      901 ctgcgcaacc gggtaaacga gctgggcgtc gccgagccga tcatccagca gcagggcgcg
      961 gaccgggtgg tcgtgcagct tcccggggtg caggatacgg cgaaggcgaa ggaaatcctc
     1021 ggccgcacgg cgaccctcga agtacgcatg gtagacgagg agaactccaa caaccccgcg
     1081 gtgctcgaag ccgccacgcg cgggcaggtg ccgttcaaca gcgagctctt ctacgagcgc
     1141 accgagcggg gcagcgtgcc gatcctggtg cgcaagcagg tggtgctgac cggggagcgc
     1201 atcaacgacg cgcagccggg cttcgacaac cagaccggcg aacccgcggt ccacatcagc
     1261 ctggatgggg ccggcgcccg catcttccag caggtgacgc gggagaacgt cggcaagcgg
     1321 atggcgatca tcctgttcga aaagggcaag ggcgaggtcg tcaccgctcc cgtgatcaga
     1381 accgaaatcg gcggcggacg ggtccagatt tcgggacgga tgaacacccg cgaagcgaac
     1441 gacgtcgcgc tgctgttgcg ggccggcgcg ctggctgctc cgatggagat catcgaggag
     1501 cgcaccgtcg gtcccagcct gggcgcggag aacatcgaaa agggcttccg cgcgacctgg
     1561 tacggcgccg cagcgatcgc ggctttcatg atcacctact acgcgctgtt cggcgtgatc
     1621 tccgtcctcg cgctggccgc caacctgatg ttcctggtcg cgctgctgtc gatgctgcag
     1681 gcgacgctga cgcttcccgg catcgcggca attgcgctca cgctcggcat ggcgatcgac
     1741 gccaacgtgc tcatcaacga gcgcgtgcgc gaagagctgc gcaacggcat gagcccgcag
     1801 gccgcgatcc acgccggcta cgaacgtgcg tgggcgacga ttctcgactc gaacatcacg
     1861 acgctgatcg cgggcatcgc gctgtttgcc ttcggctcgg ggccggtgcg cggctttgcg
     1921 gtggttcact gcctcggcat cctgacttcc atgttcagtg ccgtggtggt atcgcgctcg
     1981 atcgtgaacc tgtactacgg gcgcaagcgc cggctcgacg gcgtatcgat cgggcaggtc
     2041 tggaagccgg gcgccgaggc gcctgcgcgg taggatagga cggcgcaagg cggcagccgg
     2101 aacggagctt ccggcgccag gaaaacgaag cggcgacaag gcgaagcatg gaattcttcc
     2161 gcatcaagcg cgacatcccg ttcatgc
//
LOCUS       DASURG010000186         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_441693, whole genome shotgun sequence.
ACCESSION   DASURG010000186 DASURG010000000
VERSION     DASURG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441693"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..933
                     /locus_tag="VFY80_03110"
     CDS             121..933
                     /locus_tag="VFY80_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6006922.1"
                     /translation="MPTVLEDKIHARMPTHVWRFGLVVQRVALVGALIGLWWLVSLTM
                     PHYVMPGPPRVWDSLTALSASGDLWVNLGITLGRVATGFVFATLVGLPLGILFGANRR
                     IGDFFEPVIPVMNTVSSAIWAIFAIIWFGISNATTIFVVFMTAMPLIITNVWQGTRTV
                     NADFIELAHTLRMPSHRIMVKIYLPTILPHFFSGARLAFGFGWRVSLVAETLGSSSGV
                     GYRLRQAADLIQTDQVFAWTLTLVILMAAIEMGILRPLENYLFRWKKEAEKS"
     gene            948..1556
                     /locus_tag="VFY80_03115"
     CDS             948..1556
                     /locus_tag="VFY80_03115"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX6006923.1"
                     /translation="MRPPSAAPQRLIIAITGATGSIYGVRMLEVLREAGGWETHLIVS
                     DAGALNAWQDHRLARKDIAKLADVVHNVRDVGASIASGSFITQGMVIAPCSMKTLAGV
                     AHAFSDNLITRAADVALKERRRLVLLPREAPLNLAHLRNMVAVTEMGGIIFPPVPAFY
                     SHARTIDAMVDHTVGRVLDLFGIAHTRVKRWAGMAGSAAEKE"
     gene            1561..>2755
                     /locus_tag="VFY80_03120"
     CDS             1561..>2755
                     /locus_tag="VFY80_03120"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6006924.1"
                     /translation="MPDQATRTRSTATETFREFLERLRSVGELNDLRQPVDIRHIATL
                     VDQSDKALLFHNVIGYDMPVVSGIIRSRERAILSMGCERYAEIEEKLQRGIDRPIPPR
                     YLETSPTRQVIRVGDEVDLFTLPIPMSSIYDGGPMITAGVTIARDPEYGVNSGIYRFM
                     VKERNLTGIDIVTPNNMRLFAQRAYEAGRPCPISISIGTHPFEIMGSGFRAPLGVDEM
                     AVAGGIRGAAVELAMCETIDLPCIADAEIVLEAEILPTGWIHPEGRFGEFTRLMGGLH
                     WNPLVKVKAIAMRRDAMYYALHMPWENTWLAAPTRYQAIRRALKTAGVQVKDINVTLG
                     GCAFWHAVVSIRKQAGEGKNALLAALSVMDLKHVVVVDDDIDVFDPTDVEWAIATRVQ
                     ADRDVV"
BASE COUNT          496 a          901 c          851 g          507 t
ORIGIN      
        1 cggggcacct actacaccgc gccgccaaag tgatgcgccg gtgcactcgc cctccccttc
       61 cgttttcctg cgacctggtt tgctgccgct ctagaggtca agatcaccaa agaacaacaa
      121 atgcccaccg tactcgaaga caaaatccac gcgcgcatgc caacgcatgt gtggcgcttc
      181 gggctcgtcg tacagcgcgt ggcactggtc ggcgcactga taggtctgtg gtggctcgtg
      241 tcgctcacga tgccgcatta cgtcatgccc ggtccgccgc gcgtgtggga cagcctcacg
      301 gccctctcgg cgagcggcga tctatgggtc aatctcggca tcaccctggg ccgggttgcg
      361 accggattcg tattcgcgac gctggtcggg ctgcctctcg gtatcctgtt cggtgcgaac
      421 cgccggatcg gcgacttttt cgagccggtg atcccggtga tgaacaccgt gtcttccgct
      481 atctgggcga ttttcgccat catctggttc ggcatctcga acgcgacgac gattttcgtc
      541 gtgttcatga ccgcgatgcc cctcatcatc accaacgtgt ggcagggcac gcgcacggtg
      601 aacgccgact tcatcgagct cgcccacacg ctgcgcatgc cgagccacag gatcatggtg
      661 aagatttatc ttcccaccat cctcccccac ttcttttctg gcgcgcggct cgccttcggc
      721 ttcggctggc gcgtgtcgct ggtcgccgaa acactcggat cgtcgagcgg tgtaggatat
      781 cggctgcgtc aggcggcaga cctgatccag accgaccagg tcttcgcctg gaccctcacg
      841 ctggtcatat tgatggcggc gatcgagatg ggcatcctgc gaccgctcga gaattacctt
      901 ttccgctgga aaaaagaagc agaaaaatca tgacgcccac aaagagagtg cgcccgccaa
      961 gcgctgcgcc gcagcgcctc atcattgcga tcaccggcgc gaccggctcc atttatggcg
     1021 tccgcatgct cgaagtgctg cgcgaagcgg gcggctggga gacccacctc atcgtttccg
     1081 acgcgggtgc gctcaacgca tggcaggacc atcgcctcgc gcgcaaggac atcgcgaagc
     1141 tcgcggacgt ggtgcacaac gtgcgggacg tgggcgcaag catcgcgagc ggatcgttca
     1201 ttacgcaagg catggtgatc gcgccgtgtt cgatgaagac gctcgccggc gtcgcgcacg
     1261 cgttttccga caacctcatc acccgtgcgg ccgacgtggc gctcaaggaa cgccggcgcc
     1321 tggtcctgct gccacgcgag gcgccgctca atctcgcgca cctgcgcaac atggtcgccg
     1381 tcaccgagat gggcggcatc atttttccgc cggttcccgc cttttacagc cacgcgcgca
     1441 ccatcgacgc catggtcgat cacacggtgg ggcgcgtgct cgatctcttc ggcatcgccc
     1501 acactcgcgt gaagcgctgg gcaggcatgg cgggcagtgc ggcggaaaag gagtgacaac
     1561 atgcccgatc aggcaacgcg cacacgcagc acggcgacgg aaactttccg cgaattcctc
     1621 gaacggctgc gtagcgtcgg cgaactgaac gatctccggc agccggttga catccgccac
     1681 atcgcgaccc tcgtcgatca gtccgacaag gcgctgctct tccacaatgt gatcggttac
     1741 gacatgccgg tcgtatccgg catcatccgc tctcgtgagc gcgcaatcct ctccatgggc
     1801 tgcgagcggt atgcggaaat cgaagaaaag ctccagcgcg gcatcgatcg gccgattccg
     1861 ccccgctacc tcgaaacgtc gccaacccgg caggtgatcc gggtcggtga cgaggtcgac
     1921 ctcttcacgc ttccgatccc gatgtcctcg atctacgacg gcggcccgat gatcaccgcc
     1981 ggcgtgacca tcgccaggga ccccgagtac ggcgtgaact ccggaatcta ccgcttcatg
     2041 gtgaaggaaa ggaatctcac cggcatcgac attgtcacgc cgaacaatat gcggcttttc
     2101 gcgcagcgcg catacgaggc aggcaggccc tgtccgattt cgatctcgat tggcacccat
     2161 cccttcgaga tcatggggtc cggctttcgc gcaccgctcg gcgtggacga gatggcggtt
     2221 gccggcggca tccgcggcgc cgcggtcgag ctggcgatgt gcgaaacgat cgatctgccg
     2281 tgcatcgcag acgcggagat cgtgctggag gccgaaatcc tgcccacggg ctggatccat
     2341 ccggaagggc gcttcggcga attcacccgc ctcatgggcg ggctccactg gaatccgctc
     2401 gtgaaggtca aggcgatcgc gatgcggcgc gatgcgatgt attacgcgct gcatatgccg
     2461 tgggagaaca cctggcttgc cgcgccgacg cgctatcagg cgatccggcg ggcactgaag
     2521 accgccggcg tgcaggtgaa ggacatcaac gtgacgctcg gcggctgcgc cttctggcac
     2581 gccgtcgtct cgatccgcaa gcaggccggc gaagggaaga acgcgctgct cgctgcgctg
     2641 tcggtcatgg acctgaagca cgtcgtggtg gtggatgacg acatcgacgt cttcgacccg
     2701 acggacgtgg agtgggcgat cgccacgcgc gtacaggctg accgagatgt cgtcg
//
LOCUS       DASURG010000187         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_340163, whole genome shotgun sequence.
ACCESSION   DASURG010000187 DASURG010000000
VERSION     DASURG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340163"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFY80_03125"
     CDS             <1..333
                     /locus_tag="VFY80_03125"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6006925.1"
                     /translation="VTPAPHNASAEPTPPARAAAASAPAPNSEASAVSGIYVQFGAFR
                     SRDNAENLLARLRQQAEWLAARLHVLPREGLYRVHAGPYSNQDEARHIANRIGDMLLG
                     ARPVLLRW"
     gene            complement(338..1666)
                     /gene="hslU"
                     /locus_tag="VFY80_03130"
     CDS             complement(338..1666)
                     /gene="hslU"
                     /locus_tag="VFY80_03130"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX6006926.1"
                     /translation="MSQMTPQEIVHELDKHIIGQHDAKRAVAIALRNRWRRQQVEEPL
                     RQEITPKNILMIGPTGVGKTEIARRLARLASAPFIKVEATKFTEVGYVGRDVDTIVRD
                     LVETAIKETREQETRKVRAQAEDLAEERILDVLLPPAREGFSAETADRDSPTRQRFRK
                     KLRERELDDKEIEIEVAAVQPQMEILAPPGMEDLTSQIQSMFQNLGSGRKRLRKLRIR
                     DAMKLLTEEEAGKLVNDDDLKVRALQNVEQNGIVFLDEIDKIASRSEVSGADVSRQGV
                     QRDLLPLVEGTTVSTKYGMIRTDHILFIASGAFHFAKPSDLIPELQGRFPIRVELGSL
                     SVSDFEQILTNTDACLTRQYQALLATEGVKLEFEPEGIRRLAEIAWHVNERTENIGAR
                     RLYTVMEKLLEDVSFHAGKAGMETVRVDAAYVDGRLKDLAQSEDLARYVL"
     gene            complement(1722..>2047)
                     /gene="hslV"
                     /locus_tag="VFY80_03135"
     CDS             complement(1722..>2047)
                     /gene="hslV"
                     /locus_tag="VFY80_03135"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX6006927.1"
                     /translation="KHQGNLMRSAVELAKDWRTDRILRRLEAMLAVADRENSLIITGG
                     GDVLEPEFGLVAIGSGGPYAQAAARALLENSDFPPETIVDKALSIAGDLCIYTNQQRV
                     IEVLE"
BASE COUNT          323 a          680 c          641 g          403 t
ORIGIN      
        1 gtgactccgg cgccccacaa cgcgtcggcg gagccgaccc cgccggcgcg cgctgcggcg
       61 gcatccgcgc cggccccgaa cagcgaagct tcggcagtaa gcggcatcta cgttcagttc
      121 ggcgcgtttc gctcgcgcga caatgcggaa aacctgctcg cccgcctcag gcagcaggcg
      181 gaatggctcg cggcgcggct acatgtactg ccgcgcgagg gcctgtatcg cgtgcatgcg
      241 gggccatact cgaaccagga cgaagcgcgc cacatcgcca accgcatcgg cgacatgctc
      301 ctgggcgcgc ggcccgtcct tctcagatgg tgagcggtta caggacgtag cgggcgagat
      361 cctcgctttg cgcgaggtcc ttgagccgcc catcgacgta agccgcgtca acccgtacgg
      421 tctccatgcc ggccttgccg gcgtggaagg aaacgtcctc gagcagcttt tccatcacgg
      481 tgtagaggcg gcgtgcgccg atgttttcgg tgcgctcgtt gacgtgccat gcgatctccg
      541 cgagccgcct gatgccttca ggctcaaatt cgagcttcac gccctccgtc gcgagcagcg
      601 cctggtactg gcgtgtgagg caggcatcgg tattggtgag gatctgctcg aagtcggaga
      661 ccgacagact ccccagctcc acacgaatgg gaaagcggcc ctgcagctcc gggatgagat
      721 cggacggctt ggcgaaatga aatgcgccgc tcgcgatgaa caggatgtga tcggtgcgga
      781 tcatcccgta cttggtggat accgtcgtgc cttcgacgag cggaagaaga tcgcgttgca
      841 cgccctgacg ggaaacgtcc gcgcctgaca cttccgaacg gctcgcgatc ttgtcgatct
      901 cgtccagaaa cacgatgccg ttctgctcga cattctgcag cgcgcgaacc ttgagatcgt
      961 catcgttgac gagctttccc gcttcctctt cggtcaagag cttcatcgcg tcccggatcc
     1021 tgagcttccg caggcgcttt cggcctgagc cgaggttctg gaacatgctc tggatctgcg
     1081 aggtgagatc ctccatgccc ggcggtgcga ggatctccat ctgcggttgc acggcagcca
     1141 cttcgatctc tatttccttg tcgtcgagct cgcgctcccg cagcttcttg cggaatctct
     1201 ggcgcgtcgg gctgtcgcgg tcggcagtct cggcgctgaa gccctcgcgc gccggcggca
     1261 gcagcacgtc gaggatgcgc tcttccgcca gatcctccgc ctgcgcgcgg acctttcgcg
     1321 tctcctgctc gcgcgtttcc ttgatcgccg tttcgacgag atcgcgcacg atcgtgtcga
     1381 cgtcgcgccc gacgtaaccg acctcggtga acttcgtcgc ctcgaccttg atgaagggtg
     1441 cgctggcgag ccgcgcgagc cgccgcgcga tttccgtctt gcctaccccg gtcgggccga
     1501 tcatcagaat attcttcggc gtgatttcct ggcgcagcgg ttcctccacc tgctgccgcc
     1561 gccagcgatt gcgtagcgca atcgcgaccg cacgcttggc atcgtgctga ccgatgatgt
     1621 gcttgtccag ctcgtggacg atttcctggg gtgtcatctg agacatgaaa gcacctcgac
     1681 acgccggcgc gaaccatagg gcccgcactg cccgcgtcaa cctattccag tacctcgatc
     1741 acgcgctgct gattggtgta gatgcagagg tcgccggcga tgctgagcgc cttgtctacg
     1801 atcgtctccg gcgggaagtc cgagttttcc agcagcgcgc gcgcggccgc ctgcgcgtag
     1861 ggcccgccgc ttccgatcgc gacgagaccg aattcaggct cgagcacgtc gccgccgcct
     1921 gtgatgatga gcgaattctc gcgatcggcg acggccagca tcgcttcgag tcgccgcaga
     1981 atgcggtcgg tgcgccagtc cttggcaagc tcgaccgccg agcgcatcag gttgccctgg
     2041 tgcttgt
//
LOCUS       DASURG010000188         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_33866, whole genome shotgun sequence.
ACCESSION   DASURG010000188 DASURG010000000
VERSION     DASURG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33866"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFY80_03140"
     CDS             <1..339
                     /locus_tag="VFY80_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6006928.1"
                     /translation="VFECSGAPPAINTAIRMTSRGGRICAVAFAHEPVLVDVARLVSN
                     NIYLYGIRGEGRSATRRAAALMAQRRFPAKRLHTHTFPFADVPGAIRLAGQRAPDVIK
                     IVVKRQEVSG"
     gene            336..1244
                     /locus_tag="VFY80_03145"
     CDS             336..1244
                     /locus_tag="VFY80_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6006929.1"
                     /translation="MNDTATQKTSATTATRGCKYRFLFIQPFQLPRASRYADKYHSDA
                     LTKEQRMRMNHLNIRHLLEDVEWDFHGGPIAPYGDWAVETREEFAHVAAARLPIVREA
                     CASGKYNAIVLLGGGEPGFLESREIARRYRIPVTSCAFSQMHIATMLGNRFSVIDFAE
                     SHNMYYYDLVTRHGFASRCASIRNLNYYHARPGHEDVTTMADEREKALRGERSEAVER
                     AVEAAVDAIEEDGAEVLTFGCSDAFWLQPFLQQRLEALGWEAPVLEGYSSAIVLAKLM
                     VDLGVDASGLMFPGDRPRQWRRKKVF"
     gene            1260..>2224
                     /locus_tag="VFY80_03150"
     CDS             1260..>2224
                     /locus_tag="VFY80_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6006930.1"
                     /translation="MADEPHIAQKERNISLDPLAVREETDVPAVLPIAPYPIEARAPA
                     VREQTPAVGQRATRPDARLHGLGQTRYIDDLALPGMLYARTKRAGIASARIVSIDTHA
                     AEALPGVIAVLTGREIPVNSFGPSYQDQPVLASERVYHAGDGVAAVAATSDEIAGKAL
                     DLIRVEYEPLPAVFDPLAAMDESAPRVHPPESNIYAQKTIRKGDVDAGFAAADRIFEA
                     SYRSQMVEHVSIEPHAAMAEWDANGRVTIWCTVGRITLARADLARTLKLPLNRIRLVA
                     TVVGGNFGGKNEITQEPVLALLSKKAGRPVKGVYSRCEEFVATTTR"
BASE COUNT          404 a          703 c          749 g          368 t
ORIGIN      
        1 gttttcgagt gttccggcgc gccgcctgcg atcaacacgg cgatccgcat gacgagccgc
       61 ggcggccgta tttgcgcggt cgccttcgcg cacgagcctg tgctggtcga tgtggcgcga
      121 ctggtgagca acaacatcta cctttacggc atacgcggag aaggccggag cgccacgcgc
      181 cgggccgcgg cgctgatggc gcagcgccgt ttcccggcga aacggctgca cacgcatacg
      241 tttccattcg ccgacgtacc gggcgcgata cggcttgcag ggcagcgggc gccggacgtg
      301 atcaagatcg tggtgaagcg gcaggaggtg tccggatgaa cgatacggca acccagaaaa
      361 caagcgccac aaccgcgacg cggggctgca agtaccgctt tcttttcatc cagccctttc
      421 agctgccgcg cgcgagccgc tacgcggaca agtatcactc cgacgcgctc acaaaggagc
      481 agcggatgcg gatgaaccat ctcaacatcc ggcatctgct cgaggacgtg gagtgggatt
      541 ttcacggggg gccgatcgcg ccgtacggtg actgggcggt ggagacgcgc gaggagttcg
      601 cgcacgtggc ggcggcacgg ctcccgatcg tgcgcgaggc gtgcgcaagc ggcaagtaca
      661 acgcgatcgt gctgctcggc ggaggggagc cgggttttct cgagtcgcgc gagatcgctc
      721 gccgctatcg gattccggtg acctcctgcg cgttctccca gatgcacatc gcgacgatgc
      781 tcggcaaccg cttcagcgtg atcgactttg ccgagtcgca taacatgtat tactacgacc
      841 tcgtcacgag gcatggcttc gcgagccggt gcgcctcgat acgcaacctc aactactacc
      901 atgcgcgccc cgggcacgag gatgtgacca ccatggccga cgagcgggag aaagctttgc
      961 gcggtgaacg ctcggaggcg gtagagcgtg ccgtggaggc cgcggtggac gcgatcgaag
     1021 aagacggcgc ggaagtcctc acgttcggtt gctcggatgc attctggctg cagccgttcc
     1081 tgcagcagcg tctcgaagcg ctgggttggg aagcgccggt gctcgagggc tacagcagcg
     1141 cgatcgtgct cgccaaactg atggtcgatc tcggcgtcga tgcgagcggt ctcatgttcc
     1201 cgggtgaccg tccgcggcaa tggcgcagga agaaggtttt ttgaatcgag gctcatgaga
     1261 tggccgacga gccgcacatt gcgcaaaaag agcggaacat ctcgctcgat ccactcgcag
     1321 tccgggagga gacggacgta cctgccgtcc tgccgatcgc gccctacccg atcgaagccc
     1381 gggcgccggc ggtgcgcgaa cagacgcctg cggtgggtca gcgcgcaacc cggcccgacg
     1441 cccggctcca cgggctcggc cagacgcgct acatcgacga tctcgcgtta ccgggcatgc
     1501 tctacgcgcg caccaagcgc gccggcatcg catcggcgcg tatcgtgagc atcgatacac
     1561 atgccgccga ggcgctgccg ggcgtcatcg ccgtgctgac gggccgggaa attcccgtca
     1621 attcgttcgg accctcgtac caggatcagc cggtgcttgc gtcggaacgc gtatatcacg
     1681 ccggagatgg cgtagcggcc gtggcggcga cgagcgacga gatcgcgggg aaagctctcg
     1741 atctcatccg ggtcgagtac gagccgcttc cggcggtgtt cgatccgctg gcggccatgg
     1801 atgagagcgc ccctcgggtg catccgcccg agagcaacat ctacgcgcag aaaaccatac
     1861 gtaagggcga cgtcgatgcc ggattcgctg cggccgaccg tattttcgag gcatcgtacc
     1921 gctcgcagat ggtggaacac gtgtcgatcg agccgcacgc cgcgatggcg gaatgggatg
     1981 ccaacgggcg cgtcacgatc tggtgcaccg tggggcgcat tacgctcgcg cgcgccgacc
     2041 tcgcccgcac actcaagctt cctctcaatc gcatccgcct tgtcgccacg gtcgtcggcg
     2101 gcaacttcgg cggcaagaac gaaatcacgc aggagccggt gcttgccctg ctttcgaaga
     2161 aggcgggtag gcccgtcaag ggcgtctata gccggtgcga ggaattcgtc gctaccacga
     2221 cgcg
//
LOCUS       DASURG010000189         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_34280, whole genome shotgun sequence.
ACCESSION   DASURG010000189 DASURG010000000
VERSION     DASURG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34280"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFY80_03155"
     CDS             complement(<1..231)
                     /locus_tag="VFY80_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006931.1"
                     /translation="MADAFRQNEAGLCGTHPVPALLLKTLPLQLDEAHHRLRLPHSDQ
                     GEGSGALYLEVELSQLGVQRFRMLLKGIGLLEK"
     gene            253..504
                     /locus_tag="VFY80_03160"
     CDS             253..504
                     /locus_tag="VFY80_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006932.1"
                     /translation="MHNIGAPASSLVVVTMAPVKASIQPAAYVQSGAYLNLDFEAPDF
                     TRVQADIEYSDADGVRRRCSLTWSGDEVEIGAPNLDEAA"
     gene            complement(523..804)
                     /locus_tag="VFY80_03165"
     CDS             complement(523..804)
                     /locus_tag="VFY80_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006933.1"
                     /translation="MKTEDFFAYQDLLARAVNDIHARCGNAIDVLWDRGRLTPVHGTH
                     QVRIWNGLGAIDAEIPHSDLAAGGVAYQRFLEHIETAVKDRLTVGVGKA"
     gene            complement(958..1941)
                     /locus_tag="VFY80_03170"
     CDS             complement(958..1941)
                     /locus_tag="VFY80_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006934.1"
                     /translation="MFAACKPYRVLLSSIAIALCPAASFAQPAGLPSKQIRIVVPNAA
                     AGPNDLVARVIGPKLSETIGHPVVVENRPSPNGVIGSEYVARAVADGTVLAVGNSGTH
                     AVNATLYRNPTYDPVKDFAAISEVIFGSLVLVAHPSVKANNVRELIAEAKRAPGKLNI
                     AVAGATGEIAGNLIKMQGKIDLTNIPYKGGTPAVAAVMANESQLLLTLYSSLKGPIDS
                     GKLKPIGVTGARRIPQLPNTPTVAESGLEGYEVEFWVGVFAPAKTPAATVQALGREIA
                     RIVNLPDVKERFTTVSFEVIGSMPETFAERVRRDTEKYRKIILESGMQQLD"
     gene            2080..>2405
                     /locus_tag="VFY80_03175"
     CDS             2080..>2405
                     /locus_tag="VFY80_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006935.1"
                     /translation="MVSTFVQRFSNIRREEAGPTLVAAFFFFCVLTALMVVRPAREAL
                     GMQRGIEAIRWLFIGTVIVTLAVNPIFAWLVSRCRRLVFIAVTYLFFAAGLLGFYALL
                     VFAPAAV"
BASE COUNT          405 a          780 c          734 g          486 t
ORIGIN      
        1 tttttcgagc aagccgatgc ccttcaacag catacggaag cgctgcacgc ccagctgcga
       61 cagctcgacc tccaggtaca aggcacccga tccctcgccc tggtcgctgt gcggcaggcg
      121 gaggcgatgg tgcgcctcgt ccaactggag cggcagcgtt ttcagcaaga gagccggaac
      181 aggatgcgtg ccgcacagcc ccgcttcgtt ctggcggaag gcgtccgcca tccacgcgcc
      241 ggttcgattc gcttgcacaa tatcggagcg ccggcgtcca gccttgtggt cgtcacgatg
      301 gcgccggtca aagcgtcgat acagcctgct gcttacgtgc agagcggcgc gtatctcaat
      361 ctcgacttcg aagcgccgga cttcacccgg gtgcaggccg acatcgagta ctccgatgcc
      421 gacggcgtcc ggcgccgctg ttctttgacc tggagtggcg acgaagtgga gatcggcgcg
      481 ccgaacctcg acgaagccgc ataggttctg tcgggctgcg actcaggcct tgccgacgcc
      541 caccgtcagc ctgtccttca ccgccgtttc gatgtgctcg aggaaacgct gataggccac
      601 gccgcctgca gcgaggtccg agtgagggat ttcagcatcg atggcgccga gcccattcca
      661 gattcggacc tggtgcgtgc cgtgcaccgg ggtcagccgg ccgcgatccc acaacacatc
      721 aatcgcattc ccgcaacggg catggatatc gttcaccgct cgcgccagaa ggtcctggta
      781 ggcaaaaaag tcctcggtct tcatgaaagc tcctcgctcg caaacggagg accgggagcc
      841 ggtcttcgat gtgcctgttc tcccgcgggt gccgcctctt ttgccctccg gtgacaagag
      901 taccggagcg agccatcgcc tccttgtcct agcgcaaact tttacgcgcg atgtcgctta
      961 atcgagctgc tgcatgccgg actcgaggat gatcttgcga tatttttcgg tgtcccggcg
     1021 aacgcgctcg gcaaaggttt ccggcatgct gccgatcact tcgaagctca cggtggtgaa
     1081 gcgctccttc acgtccggaa ggttgacgat gcgagcgatt tcccggccca acgcctgcac
     1141 cgtcgccgcg ggcgttttcg ccggcgcaaa aacgccgacc cagaattcga cctcgtagcc
     1201 ttcgagcccg ctttctgcga ccgtcggcgt attgggaagc tgaggaatgc gccgcgcgcc
     1261 cgtgacgccg atcggtttca gtttgccgga atcgatcggc cccttgaggc tcgaatagag
     1321 ggttagcagc agttgcgatt cgttggccat caccgcagcc acggccggcg tgccgccctt
     1381 gtacgggatg ttggtgagat cgatcttgcc ctgcatcttg atgagatttc cggcgatctc
     1441 accggtcgcg cccgcgaccg cgatattgag cttgccggga gcccgcttcg cttcggcgat
     1501 gagttccctc acgttgttcg ccttcaccga cgggtgcgcg accagcacga ggctgccgaa
     1561 aatgacctcg ctgattgcag cgaagtcctt caccggatcg taggtcggat tcctgtagag
     1621 cgtggcgttg accgcgtgcg tgccggaatt gccgacggcg agcactgttc cgtccgctac
     1681 ggcgcgagcc acgtattccg agccgatcac gccgttagga ctggggcgat tctccacgac
     1741 gaccgggtgc ccgatcgtct cgctcagctt cgggccgatc acgcgcgcca cgagatcgtt
     1801 cggccccgcc gcggcattcg gaaccacgat ccgaatctgc ttggagggaa ggcccgccgg
     1861 ctgagcgaac gacgcagcgg ggcacagcgc aatggcaatg gaagacagca gcactctata
     1921 tggcttgcat gcggcaaaca tctgtaggct cctcgtctgg taatccggtc cagcgcctat
     1981 gctaccgcat cggacgagcc ggctcgcgta caccggtcca cggccggcgt gccttcatgc
     2041 gtttcaggta agatcactac ccttcgcgag ccaagatcag tggtatccac tttcgtccag
     2101 cgattctcca acatacgccg ggaagaagcg ggaccgacgt tggtcgcggc ctttttcttc
     2161 ttctgcgtcc tcacggcgct catggtcgtg cggccggcgc gcgaagcgct cggcatgcaa
     2221 cgtggcatcg aggcgatacg ctggcttttc atcggcacgg tgatcgtgac gctcgcggtc
     2281 aacccgattt tcgcctggct cgtgagccgc tgccgacggc tcgtgttcat cgccgtcact
     2341 taccttttct tcgccgcagg cctgctcggg ttttatgcgt tgctcgtatt cgcgccggcc
     2401 gcggt
//
LOCUS       DASURG010000190         6208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1054850, whole genome shotgun sequence.
ACCESSION   DASURG010000190 DASURG010000000
VERSION     DASURG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054850"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFY80_03180"
     CDS             complement(<1..349)
                     /locus_tag="VFY80_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006936.1"
                     /translation="MPLLTARTIRRRAPVGAEVQPGGGVHFRTWAPLADRVEVVLQSA
                     ALPQARLARALEKKAGGYFCALIADASPGMRYRFRLDGGEALPDPASRFQPDGPHGDS
                     VIIDPATFVWTDQA"
     gene            complement(437..1111)
                     /locus_tag="VFY80_03185"
     CDS             complement(437..1111)
                     /locus_tag="VFY80_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HEX6006937.1"
                     /translation="MAHDLLGSLRHFLERFRHMGYELVRSINAAAITGFHDAALSPIT
                     VNCAPPRAAFAALWRAIADNHRCNALLSLEEERASRIPIGDPDLSVCRYRIDRYNVER
                     SAAVAAIDETLLDVLADVPRHRAARLYTETAGSMIDRLSTLAIRIHRMRLYSERHARD
                     GMLMPHYLSGLAALVAQRVNLACCFDEFIAGASLGIAYFSMSRRRRDREGEPQRHPAK
                     QRDFVS"
     gene            1235..1654
                     /locus_tag="VFY80_03190"
     CDS             1235..1654
                     /locus_tag="VFY80_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6006938.1"
                     /translation="MSHDVRVANPDQTIRDAARMMSEIDAGVLPVGENDRLVGMITDR
                     DIAVRAVAQDKPPSTKVRDVMSREVLYCYDDQDLDEVAVNMGDEQVRRMPVVNRDKRL
                     VGIISLGDLAQTANAKTAGRAVADISSPGGPHTQRAN"
     gene            1741..3375
                     /locus_tag="VFY80_03195"
     CDS             1741..3375
                     /locus_tag="VFY80_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX6006939.1"
                     /translation="MLDLWYKNTIVYSLNVAAYMDGNDDGIGDFKGLAQRIDHIAKLG
                     VNCIWLLPFYPSPRLDHGYDVTNYYDVAPELGTLGDFVEFSHQARLRGLRIVIDLPIN
                     HTSDQHPWFRQARSNPHSPYRDYYIWSHAKPANTNDGVVFPGYQSSVWTYDPRARAWY
                     FHRFYPHQPDLNISCPQVREEIFKIVGFWLELGVSGFRIDAAPFVVQEVSVDRPVERR
                     YEFFSELRNFLSWRKGDAILLAEANVLPDEMPEYFGDGARLHMMFAFLVNQYAFLTFA
                     RHRAEPLVHGLKQLPTLPQLGQWAHFLRNHDELDLGRLTAEERYECYEAFAPDPDMRL
                     YERGIRRRLAAMFDGDRRRIELAFSLIFSLPGAPVIYYGDEIGMGDALELPERWPVRT
                     CMQWSDEDNGGFSTVAEERLIHPVIMDGPYGARRVNALAQQRDPDSLLNWMRRLADVR
                     QSCPEIGWGEMAIMESADPAVFAHRMSWNGNTTVALHNLGGRPCRVRVALNDEEAGEL
                     TDVFGNKVYERDTGENRVFALDGYGYRWFRLDGVRH"
     gene            3423..5054
                     /locus_tag="VFY80_03200"
     CDS             3423..5054
                     /locus_tag="VFY80_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6006940.1"
                     /translation="MEQAKRTDIEWWKHGVVYQIYPRSFQDSNGDGIGDLRGIIERLD
                     YLAWLGVDALWLSPIYPSPMADFGYDITDFRGIDPIFGSLDEFDDLVRRAHDRGLKVI
                     LDFVPNHTSDQHPWFRESRRSRGSPRREWYVWRNPGPGGGPPNNWLSEFGGSAWEFDE
                     PTGQYYYHAFLKEQPDLNWRNPEVRREMADVLRFWLERGVDGFRIDVVHHLVEDAAFR
                     DNPPNTRFEQGMRPSHALLSTHTVDQPEVQEIITEWRRVADEYGDRVLIGEIHLPIDR
                     LVAYYGVELNGIHLPFNFNLIGAPWSAAVLGDIITAYEAALPRGAWPNWVLGNHDTLR
                     IASRVGQAGARLAAMLLLTLRGTPTVYYGDEIGMEDVDLPREAVRDPFELNVPGIGVG
                     RDPARTPMRWTSDAGQCGFTTGVPWLPIGERCASCNVRTQSADPTSMLTFCRALLKLR
                     REQPALALGDYRAVPCEDDCLAYARRYDEARVLVVLNFSERARDLTIPSEFASGRVLI
                     SSGMTRKNARVGRSVELAPVEGVVIGAHDGGDTSV"
     gene            5073..5255
                     /locus_tag="VFY80_03205"
     CDS             5073..5255
                     /locus_tag="VFY80_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006941.1"
                     /translation="MIYRRRVSESLWHFCRNCSSWPSERYEEGTIEPPREALCAECIR
                     KNDELKCEVMPQPGAA"
     gene            complement(5256..6170)
                     /locus_tag="VFY80_03210"
     CDS             complement(5256..6170)
                     /locus_tag="VFY80_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6006942.1"
                     /translation="MRTSLRNAWQRTRAAVALWIDRSAARLGAAVAFYSIFSLAPLLV
                     IAVTLAGAFLGPDFAREQFLAQVAALMGDRAASVVDTMIDAALESGKGGWAGVTSAVT
                     LFIGATGVLVELRNALDAIHRTEAAAGLSAMVRARLWAIALVLAIGFLLLLSLIASAV
                     IAAMGAWIIDRYPVLAVLTMVLNWVLSLGILAALFGLLLRWLPSQRQTWSSVWPGAVL
                     ASILMTVGKELLGLYLGRAAFSDAFGAAGSLVVLVMWIYYSVQVFLFGAAYNEVRSEA
                     RRTQATATKAVAAGYHPSERSDAAPRAG"
BASE COUNT         1161 a         1988 c         2010 g         1049 t
ORIGIN      
        1 aagcttgatc ggtccagacg aatgtcgccg gatcgatgat gacggagtcg ccgtgcggac
       61 cgtccggttg gaaacgcgac gccggatcgg ggagcgcttc gccgccgtcg aggcggaagc
      121 ggtagcgcat tccggggctt gcgtcggcaa tcaaggcgca aaaatagccg ccggccttct
      181 tctcgagcgc ccgggcaagg cgagcctggg gtagggcagc ggattgcaat accacttcga
      241 cgcgatcggc aaggggcgcc caagttctga agtgcacgcc gcctcccggc tgcacttcgg
      301 cgccgaccgg cgcacgtcgc cggatagtgc gtgcagtgag caagggcacg caggcggaat
      361 cgttcatcgc aacgaccccc gacgcctcgc ctgttgacga tcacgcggcc cgggtaccgg
      421 gtcgcgttgt cgtgcgtcag gaaacaaagt cgcgttgttt cgccggatgc cgctgtggct
      481 cgccttcgcg gtcgcgccgt ctgcgggaca tgctgaaata cgcgatgccg agtgacgcgc
      541 cggcgatgaa ttcgtcgaag cagcacgcca gatttacccg ctgcgccacg agtgcggcga
      601 ggccggagag atagtgcggc atgagcatgc cgtcccgcgc gtgccgttcc gagtagaggc
      661 gcatgcggtg aatgcggatt gcgagcgtgg agagccggtc gatcatgctg cccgcggttt
      721 cggtatagag gcgtgcggcc cggtgcctcg gcacgtcggc cagcacgtcg agcagtgtct
      781 cgtcgatcgc cgcgactgcg gcgctgcgct cgacgttata gcgatcgatg cggtatcgac
      841 acacggagag gtccgggtct ccaatcggaa tgcggctcgc gcgttcttct tccagcgaga
      901 gcagcgcatt gcaccgatgg ttgtccgcaa tcgcgcgcca cagcgccgca aacgccgccc
      961 gcggcggggc acaattcacg gtgatcggcg acaatgcggc atcgtgaaaa ccggtgatcg
     1021 ccgccgcgtt gatgctcctg acaagctcgt agcccatgtg ccggaatcgc tcgaggaagt
     1081 ggcgcagtga gccaagcaag tcatgtgcca tgagcaccga tcccaccggg acgcaggcac
     1141 agtgattgct tgaatgcgaa agtgcgttcc gggaatccgg gcgcgcaaaa gtgcaatctt
     1201 ttgtcaggag ccacatatgc gagtaagcga agccatgagt catgacgtca gagtggcgaa
     1261 tccggatcag acgatacgcg atgccgcccg catgatgagc gaaatcgacg ccggcgtgct
     1321 gccggtgggc gagaacgacc gcctggtcgg catgatcacc gatcgcgaca tcgccgtgcg
     1381 cgcagtcgcg caggataaac cgccgagcac caaggtccgc gacgtgatga gtcgcgaggt
     1441 gctctattgc tacgacgacc aggacctgga cgaagttgct gtaaatatgg gagacgagca
     1501 ggtgcggcgc atgccggtgg tgaaccgcga caagcgtctg gtcggcatca tctcgctcgg
     1561 cgatctcgcg caaaccgcga acgcgaaaac cgctggtcgc gcggtggcgg acatctcttc
     1621 gcccggcggc ccgcacacgc aaagggcgaa ctgacgactt taccgcgggc gcgcaagaag
     1681 cacgcccgcg acttcaattg cgctcggcgt ccgtgccgca cgagccgacg gagctgcagc
     1741 gtgctcgatc tctggtacaa gaacacgatc gtctacagcc tcaacgtcgc agcctacatg
     1801 gatggcaatg acgacggcat cggagacttc aaagggctcg cccagcgcat cgaccatatc
     1861 gccaagctgg gggtaaattg catctggctg ctcccgttct acccgagccc ccggctcgac
     1921 catgggtacg acgttaccaa ttactatgac gtcgcccccg agctcggaac gctcggcgat
     1981 ttcgtcgagt tcagccacca ggctcggctc cggggcctga ggatcgtgat cgatctcccg
     2041 atcaatcaca cgtccgacca acacccgtgg tttcgacagg cccggtcgaa tccgcattcg
     2101 ccgtatcgcg actactatat ctggtcgcat gccaagccgg ccaataccaa cgacggcgtg
     2161 gtattcccgg gataccagtc gagcgtctgg acctacgatc cgcgcgcgcg tgcgtggtat
     2221 ttccatcgct tctatccgca ccagccggat ctgaacatct catgtcccca ggtgcgcgag
     2281 gaaatcttca agatcgtagg gttctggctc gaactgggcg tatccggctt tcgcatcgat
     2341 gccgcgcctt tcgtggtgca ggaagtgagc gtcgaccgcc cggtggagcg acgctacgag
     2401 ttcttcagcg agctgcgcaa ctttctctcg tggcgcaaag gcgacgccat cctgctcgcg
     2461 gaagcgaacg tgctgcctga tgagatgccc gaatacttcg gcgacggtgc gaggctgcac
     2521 atgatgttcg ccttcctcgt caatcagtac gcgtttctca cgttcgcgcg gcatcgtgcc
     2581 gagccgctgg tgcacggcct gaaacagctt ccgacgctgc cgcagctcgg ccagtgggca
     2641 catttcctgc gtaaccacga tgagctcgac ctcgggcggc tcaccgccga ggagcgctac
     2701 gaatgttacg aggcattcgc accggacccc gacatgcggc tctacgagcg cggcatccgg
     2761 cggcgcctcg cggcgatgtt cgacggcgac cggcggcgca tcgagctcgc cttcagcctc
     2821 atcttcagcc tgccgggcgc gcctgtcatc tactacgggg acgaaatcgg catgggcgac
     2881 gcgctcgagc tgccggagcg ctggccggtt cggacctgca tgcagtggtc cgacgaagac
     2941 aatggcggat tctccaccgt cgcggaagaa cgcctcatcc atcccgtaat catggacggc
     3001 ccgtacggtg cgcgccgcgt caatgcgctg gcgcagcagc gcgatcccga ttctctgctc
     3061 aactggatgc ggcgtctcgc cgacgtgcgc caatcctgcc ccgagatcgg ctggggcgag
     3121 atggcgatca tggaaagcgc cgatccggcc gttttcgcgc atcgcatgag ctggaatggc
     3181 aataccaccg tcgcgcttca caatctcggc ggccggccct gccgcgtccg ggttgcgctg
     3241 aacgacgagg aagcgggcga actcaccgat gtcttcggca acaaagtgta tgagcgcgac
     3301 accggcgaga accgggtatt cgcgctggac ggctacggtt atcgctggtt tcggctggac
     3361 ggcgtgcgcc actgatccgc gcggcgccgg ccggcgcacg ctcacctacc agaccgggag
     3421 agatggaaca ggcgaagcgc accgacattg agtggtggaa gcacggcgtg gtgtaccaga
     3481 tatatccgcg ttccttccag gactccaacg gggatggcat cggcgacctc aggggaatca
     3541 tcgagcgact cgactatctc gcatggctgg gggtcgatgc cctatggctc tctccgattt
     3601 atccgtcgcc gatggccgat ttcggctacg acatcaccga tttccggggc atcgatccca
     3661 tcttcggtag cctcgacgaa ttcgacgatc tcgtacggcg cgcgcatgat cgcgggctca
     3721 aggtgatact ggacttcgtg ccgaatcaca cttcggatca acacccctgg ttcagggaat
     3781 cccgccgcag tcgcggcagc ccgcgacggg agtggtacgt ctggcgcaat cccggcccgg
     3841 gcggcggacc gccgaacaac tggctgagcg agttcggcgg ctcggcctgg gaattcgacg
     3901 agccgaccgg acagtactac taccacgcgt ttctcaagga gcagccggat ctcaactggc
     3961 gaaatccgga ggtgcgacgc gaaatggccg acgtgctgcg cttctggctc gagcgcggcg
     4021 tggacggctt tcgcatcgat gtcgtgcatc atctcgtcga agatgctgca ttccgcgaca
     4081 acccgccgaa tacgaggttc gagcagggca tgcggccaag ccacgcgctc ttgagcacgc
     4141 ataccgtgga tcagccggaa gtgcaggaga tcatcacgga gtggcggcgt gttgcggatg
     4201 aatacggcga ccgggtgctg atcggcgaga ttcacctgcc gatcgatcgc ctcgtggcct
     4261 actatggtgt ggagctgaac ggcatacacc tgccattcaa cttcaacctc atcggcgcgc
     4321 cttggtcggc cgcggtgctg ggcgacatca tcactgcgta cgaggccgcg ctgccccgcg
     4381 gggcatggcc gaactgggtg ctcggcaatc acgatacgct acgcatcgcc tcgcgcgtag
     4441 gtcaagccgg ggcccggctc gcggccatgc tgcttctcac gctgcgcggc acgccaaccg
     4501 tgtactacgg cgacgagatc ggcatggagg acgttgacct cccccgtgaa gcggtgcgcg
     4561 accctttcga gctgaacgtt ccgggcatag gggtgggaag agatcccgcg cgcactccaa
     4621 tgcgctggac gtccgacgca gggcaatgcg gcttcaccac cggcgtgccg tggctgccga
     4681 tcggagagag gtgtgcaagc tgcaacgttc gcacgcagtc cgcggacccg acttcgatgc
     4741 tgactttctg ccgggcactc ctcaagctgc ggcgcgagca gcccgcgctc gcgctgggcg
     4801 actatcgggc cgttccctgc gaagacgact gcctcgccta cgcccgacgc tacgatgagg
     4861 cacgggtgct tgtcgtgctc aatttttcgg aacgcgcgcg cgatctgacg atcccttctg
     4921 aattcgcatc cggccgggtt ctgatctcaa gcggcatgac gcgcaagaac gcacgtgtcg
     4981 ggcgctccgt ggagctcgcg ccggtagaag gcgtcgttat cggcgcccat gacggcggcg
     5041 acacttccgt gtagcacggc tgtcgaatcg tcatgatcta ccggcgccgc gtttccgagt
     5101 cgctgtggca cttctgtcgc aattgctcca gctggccgag cgagcgatac gaagagggca
     5161 cgattgagcc gccgcgcgag gcgctgtgcg ccgagtgcat acgcaagaac gacgagctca
     5221 agtgcgaagt gatgccacag cccggcgcgg cgtgattagc ctgcccgagg cgcggcgtcg
     5281 ctccgctcgg agggatgata gccggccgct accgctttcg tcgcggtcgc ctgcgtccga
     5341 cgcgcttcgc tgcggacttc gttgtaggcg gcgccgaaca gaaacacctg caccgaataa
     5401 tagatccaca tcaccagcac cacgagcgag ccggcggcac cgaacgcgtc cgagaatgcc
     5461 gcgcggccca gataaagacc aagcagttcc ttgccgaccg tcatgaggat gcttgccagc
     5521 accgcacccg gccacacgct ggaccacgtc tggcgctggg agggcagcca gcgcaggagc
     5581 agcccgaaca gcgcggcgag gatgccgaga gacagcaccc agttgagcac cattgtgagg
     5641 acagcgagca cgggatagcg atcgatgatc caggcgccca tcgcggcgat aaccgcgctg
     5701 gcgatgagcg acaggaggag gagaaatccg atcgccagga ccaacgcgat cgcccagagg
     5761 cgtgcgcgca ccatggcaga gagccccgcc gcggcctccg tgcggtggat cgcatcgagc
     5821 gcattgcgta gctcgaccag cacgccggtc gcgccgatga aaagcgtcac cgcgctcgta
     5881 acccctgccc acccgccctt gcccgactcg agcgccgcat cgatcatggt atcgacgacg
     5941 ctcgccgccc tgtctcccat gagggccgcc acctgcgcga gaaactgctc gcgcgcgaag
     6001 tccgggccga gaaaggcgcc ggcgagcgta acggcaatca cgagtagcgg agcgagcgag
     6061 aaaatcgagt agaaggcgac cgccgctccc agccgcgctg cactgcggtc gatccaaagg
     6121 gcgacggccg ctctggtgcg ttgccaggca tttctcagcg atgtccgcat ttcagcgctt
     6181 tagcccaggg tcccgcgtcg atgcatga
//
LOCUS       DASURG010000191         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_34982, whole genome shotgun sequence.
ACCESSION   DASURG010000191 DASURG010000000
VERSION     DASURG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34982"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFY80_03215"
     CDS             complement(<1..844)
                     /locus_tag="VFY80_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6006943.1"
                     /translation="MAIRGRGIPEVGEVTIAGNDPVFSARFKIGETTANILAGVGVAV
                     NDIHEMKTGRRQKVAIDVRHAGATCQSSKLMKKREPDGEWKVVQSPFMEHMRSITQPW
                     RTKDGRWYLPHFNLPHLHDRVIGVLKCESNADAVSNAIAKWDSHELEEAVAAARACGS
                     IVRSNAEWLQHPQGKVLSGKPLVTITKIGESAPIPFPEGGRPLSGIRVLDLTRILAGP
                     MAARTLAEHGADVLMVGAAHLPQVPEHVMDTSHGKRSCFLDLRQADDLATIKRLVTKV
                     DVFSQ"
     gene            1008..2024
                     /locus_tag="VFY80_03220"
     CDS             1008..2024
                     /locus_tag="VFY80_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX6006944.1"
                     /translation="MATTSERLDADRPDTARLTVAEATELGETALKRVGYSGEDATII
                     VDQLVDNALCGYRFAGLPRLLAIASDERTGRKREPVRIVHETNVSALLDGGNNVGYIA
                     AYRAAEVAIRKACESGMASVGAYNSYYSGRNAYFVEKIVREGLVAFHVSSAYPRVLPP
                     GAARPMLGTNPISFGFPSTNGPVIVDMGTASFMWGEVMLHAHLGKPLPEGVGFDEHGN
                     PTRDAQAALKGGVAPFGGHKGYALSFAIQALGLLAGAALTRGQVRDYGFLFWVLDPNM
                     MLPGGEFASQMAEYVRQIRDAPRRSSVEQIRVPSERAFRERERRRKEGIVIERKVVEA
                     LRAL"
     gene            complement(2167..>2418)
                     /locus_tag="VFY80_03225"
     CDS             complement(2167..>2418)
                     /locus_tag="VFY80_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6006945.1"
                     /translation="VHFAAAIPAHVRGHVEWDPTLGNPLVEKMLTNPLQVVDGHIMVP
                     QGPGLGTDINWDVVKELPFSAGEEIKGHGKRRARRWNTL"
BASE COUNT          425 a          789 c          755 g          449 t
ORIGIN      
        1 cctggctgaa gacgtcgact ttcgtcacca gtctcttgat cgtcgcgagg tcgtccgctt
       61 gcctcagatc gagaaagcaa ctgcgcttgc cgtggctcgt atccatcacg tgctcgggga
      121 cctgcggcag gtgcgccgct ccgaccatga gcacgtcggc gccgtgctcg gccagcgtgc
      181 gcgcggccat cggtcccgcg agaatacgcg tcagatcgag cacgcggatg cccgagagcg
      241 gccgcccgcc ttcgggaaaa gggatcgggg cgctctcgcc gatcttggta atcgtgacga
      301 gcggcttgcc ggagagcact ttaccctgcg gatgctgaag ccactcggcg ttggaacgca
      361 caatcgaccc acatgcgcgc gccgcggcga ccgcttcttc cagctcgtgc gaatcccatt
      421 ttgcaatcgc gttggaaacg gcatccgcgt tcgactcgca cttcaacacc ccgatcacgc
      481 ggtcgtggag atgcggcagg ttgaaatgcg gcagatacca gcggccgtcc ttcgtgcgcc
      541 atggctgggt gatcgagcgc atgtgctcca tgaacggcga ctgcacgact ttccattcgc
      601 catccggctc gcgtttcttc atgagcttcg agctctggca cgttgcgccc gcatgacgga
      661 catcgatcgc gaccttctgg cgccggccgg tcttcatctc gtggatgtcg ttcacggcaa
      721 ctccgacacc agcgaggatg ttcgccgtcg tctcgccaat cttgaaacgc gcggaaaaga
      781 ccgggtcatt tccagcgatc gtcacttcgc cgacctcggg tatgccgcgg ccgcggatcg
      841 ccattacttc ttcgaatccg gagttcattg tgtggaagat cgacgagatg aacggagaac
      901 tcgatttaca gcattacggg cgccttgtcg agccgcaata atggggacag accctcattt
      961 ctcatccctt tcgagaaaac ggggtctgtc ccctattatt gtctgccatg gcaactacgt
     1021 ctgagcggct ggacgccgac cggcccgaca cggcgaggct cacggtcgcc gaagcgaccg
     1081 agctcggcga gacggctttg aagcgcgtcg gctacagcgg cgaggatgcg accatcatcg
     1141 tcgatcagct cgtggacaac gccctctgcg gctatcgctt cgccggcttg ccgcgcctcc
     1201 ttgccatcgc gagcgacgag cgcaccggcc gcaagcgcga gccggtccgc atcgtgcacg
     1261 agacaaacgt atccgcgctc ctcgacggcg gcaacaacgt cggctacatc gccgcatacc
     1321 gcgccgccga agtcgcgatc cgcaaggcgt gcgagtccgg catggcgtcc gtcggcgcct
     1381 acaacagcta ctacagtggc cgaaatgctt acttcgtcga gaagatcgtg cgcgagggtt
     1441 tggtcgcgtt ccacgtctcg agcgcctacc cgcgtgtgct accgccgggt gcagcgcgtc
     1501 ccatgctcgg caccaacccg atctctttcg gctttccctc gacgaacggc ccggtgatcg
     1561 tggacatggg caccgcctcc ttcatgtggg gcgaagtgat gctgcacgcg catctcggaa
     1621 agccgctccc tgaaggtgtc ggcttcgatg agcacggcaa tcccacgcgc gatgcgcaag
     1681 cggcgctcaa aggcggcgtc gcaccgttcg gcggccacaa gggctacgcg ctctccttcg
     1741 cgatccaggc gctggggctc ctcgcgggcg cagcgttgac gcggggacag gtgcgggact
     1801 acgggttcct cttctgggtg ctcgatccga acatgatgct gcccggcgga gagttcgcga
     1861 gccagatggc ggaatacgtg cggcagatca gggacgcgcc gcgacggtcc agcgtggagc
     1921 aaatccgcgt gccgtcggag cgcgcgtttc gcgagcgcga gcggcggcgc aaggaaggca
     1981 tcgtcatcga gcgcaaagtc gtagaagccc tccgcgcgct ttagcggttc gtcacaccgg
     2041 cgaaagccgg tatccggaag acatgcgtgc cttgctagat gcggttttca ccggcatgac
     2101 gagtcccaca atgatccggt tgtcagcggg acaccacatc agagcgccgc ggtgaaagca
     2161 ccttctttat agggtgttcc agcggcgcgc gcgacgcttg ccgtgcccct tgatctcttc
     2221 acccgcggag aagggcagtt ctttcacgac atcccagtta atgtcggtgc cgagccccgg
     2281 accctgcggc accatgatgt gaccgtccac gacctgcagc ggattcgtca gcatcttctc
     2341 caccagcgga ttgccgagtg tcggatccca ttcgacgtgc ccgcgcacgt gggcgggaat
     2401 ggccgcggcg aagtgcac
//
LOCUS       DASURG010000192         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_35020, whole genome shotgun sequence.
ACCESSION   DASURG010000192 DASURG010000000
VERSION     DASURG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35020"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFY80_03230"
     CDS             complement(<1..925)
                     /locus_tag="VFY80_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006946.1"
                     /translation="MTFPTRPIGIALAALLAALVSPVAAAQGGYPAKPIRWIVPWPPG
                     GGADVLSRMLSPKLSDALKQQIVIDNRGGAAGNIGAEMAAKSPPDGYTIVFAYSGTHA
                     INPSIYSRMPFKESDFAPIIWLLSVPQVLVVHPSLPVKNVKDLVALAKKRPGQLTYGS
                     SGNGAFNHLTGALFSNTAKIDIVHVPYKGGGPAAVALLSGEITMILGEPASIVGFVKA
                     GKVRALAVTGEKRALGMPELPTMVEAGIPGYSATSWNGMLAPAGVPAPIIKRLNDEFN
                     RIITDAEIRKRMLDNGYEPVGGSPEKFAAHIR"
     gene            complement(936..1979)
                     /locus_tag="VFY80_03235"
     CDS             complement(936..1979)
                     /locus_tag="VFY80_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6006947.1"
                     /translation="MKARALVAGAGGLIGRRIAAHLLERGWDVAGIARRRHSVPPLSW
                     IATDLTDTNKTRTALATLNDVTHVFYAARADHPEGVPESESRNTAMLRNLLDALSLAG
                     SPLAHVTLVHGTKHYGHPDPIPFPVSEELPRGARPTFYFAQEDLVRSRATEGGWTWSI
                     ARPHTFCDPAPDHPRSIGLVIAIYAAIQRALGEPLFFPGSERSFNARTQFTDTALLAR
                     ALEWMGTEPRCANQAFNIVNGDVPRWCEMWPRFADMLGIEPGGPRAMNLACYMRDKEG
                     VWQALVQRHGLRAAPLERIVLWDYGDYVFRPEWDIMSAMNKARRCGFEPRVDTLEMFS
                     RVFAGYREARVIP"
     gene            complement(1982..>2201)
                     /locus_tag="VFY80_03240"
     CDS             complement(1982..>2201)
                     /locus_tag="VFY80_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006948.1"
                     /translation="DAPAVEAGQILLVADREREDAGLAHLGITWLGCAGIDCSTPLRG
                     IAVVDETTRLPAGVARYRPVTAHLRPAR"
BASE COUNT          367 a          723 c          732 g          379 t
ORIGIN      
        1 cgcgtatatg ggcggcgaac ttctccggcg atccaccgac gggctcgtaa ccgttgtcga
       61 gcatgcgttt cctgatttcc gcgtcggtga tgatgcggtt gaactcgtcg ttcaggcgct
      121 tgatgatggg cgccggcacg cccgccgggg ccagcatgcc gttccacgag gtcgcggaat
      181 aacccggtat gccggcttcc accatcgtcg gcagttccgg catgccgagg gcgcgttttt
      241 ccccggtcac ggcgagcgcg cgcaccttgc ctgccttgac gaagcccacg atcgatgcgg
      301 gctcgccgag gatcatcgtg atttcacccg aaagcagtgc caccgccgcc gggccgccgc
      361 ctttgtacgg cacgtgcacg atatcgatct tcgccgtgtt cgagaagagc gcgccggtca
      421 agtggttgaa ggcaccgttt ccgctcgaac cgtaggtaag ctgccccgga cgcttcttcg
      481 cgagcgcgac gaggtccttc acattcttga ccgggagcga aggatgaacc accagaacct
      541 gcggaaccga caagagccag atgatcgggg cgaaatcgct ctccttgaac ggcatcctcg
      601 agtagatgct gggattgatc gcgtgcgtgc ccgagtaggc gaacacgatg gtgtaaccgt
      661 cgggcggaga cttcgccgcc atttcggcgc cgatgttgcc cgcggcgccg ccgcgattgt
      721 cgatgacgat ctgctgcttg agggcgtccg agagcttcgg gctcagcatg cgtgagagga
      781 catccgcgcc gccgcccgga ggccacggca cgatccagcg gatgggtttg gcggggtagc
      841 cgccctgtgc cgctgcgacc gggctcacga gcgccgcgag cagcgcagca agcgcaatgc
      901 cgatgggtct tgtcgggaag gtcatggaat cctcctcacg gaatgacgcg cgcctcgcgg
      961 tagcctgcga agacgcggct gaacatttct agggtatcga cgcgcggctc gaatccgcag
     1021 cggcgcgcct tgttcatcgc cgacattatg tcccattcgg gtcggaacac atagtcgccg
     1081 tagtcccaca gcacgatgcg ttcgagcgga gcggcgcgca gtccgtgacg ttggacaagt
     1141 gcctgccata cgccttcctt gtcgcgcatg tagcacgcga ggttcatcgc acgcggcccg
     1201 cccggctcga tgcccagcat gtccgcgaac cgcggccaca tttcgcacca ccgcggcaca
     1261 tcgccattca cgatgttgaa cgcctggttc gcgcagcgcg gttcggtccc catccattcg
     1321 agcgcgcgcg ccagaagcgc ggtatcggtg aactgcgttc gcgcgttgaa gcttctctcg
     1381 gaccccggaa agaaaagcgg ctcgcccagc gcccgctgga tcgcagcgta aatggcgatc
     1441 acgagcccga tcgaacgcgg atgatcgggt gccggatcgc agaaggtgtg cggccgggcg
     1501 atcgaccacg tccagccccc ttcggtcgcc cggctgcgca cgagatcttc ctgcgcgaag
     1561 tagaacgtcg gacgcgcgcc gcgcggcagc tcttcggata ctggaaacgg aatggggtcg
     1621 gggtggccgt agtgcttcgt gccgtgcacg agcgtgacgt gggccagtgg cgagccggcc
     1681 agcgaaagcg catcgagcag gttgcgcagc atcgcggtgt tgcggctctc gctctccgga
     1741 acgccttcgg ggtgatcggc gcgcgccgca tagaaaacgt gcgtgacgtc gttcagcgtt
     1801 gcaagcgctg tgcgcgtctt gttcgtgtcc gtcagatccg tcgcgatcca tgaaagcggc
     1861 gggacggaat gccgacgacg cgcgatccct gccacatccc agccgcgctc gagaagatgc
     1921 gcagcgatgc gccgtccgat gagtccgccg gcgccggcga cgagcgcccg tgccttcatg
     1981 cttaccgcgc gggccggagg tgagcggtca ccggccggta gcgcgcgacg ccggccggca
     2041 gccgggtcgt ctcgtccacg accgcgatgc cgcggagcgg tgtcgagcag tcgatgcctg
     2101 cgcatccaag ccacgtaatc ccgaggtgcg cgagccccgc atcctcgcgc tcgcggtccg
     2161 caaccagcaa tatctgtccc gcctccacag caggtgcgtc c
//
LOCUS       DASURG010000193         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_239601, whole genome shotgun sequence.
ACCESSION   DASURG010000193 DASURG010000000
VERSION     DASURG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239601"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFY80_03245"
     CDS             <1..282
                     /locus_tag="VFY80_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006949.1"
                     /translation="AAPEFPTISEAGVPAYHHQSWVGMLAPAKTPRPIITRLNSEVVR
                     IVQAPDMKSLLLRDGLEAAGDKPEEFEKDIKEEIAKWQKLTKAAGIKAE"
     gene            360..1100
                     /locus_tag="VFY80_03250"
     CDS             360..1100
                     /locus_tag="VFY80_03250"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEX6006950.1"
                     /translation="MDELARFCELVRAARNGVAFTGAGISTESGIPDFRGPQGVWTTE
                     TPVLYQDFMADRAARVRAWERGVRMFHRCKSARPNEGHLALAQLERRGHIAAVITQNI
                     DGLHRDAGSTEVVELHGTNRYSACQHCGKEWTTAQIVARVERGEEAPECDDCRGPIKT
                     RTISFGQSMPETEMRRAAELSVAADLFVSIGSSLVVEPAASFPRLARHSGAKLVILNN
                     QETPLDDLADLVIRESIGVTLRTLLARL"
     gene            complement(1221..>2177)
                     /locus_tag="VFY80_03255"
     CDS             complement(1221..>2177)
                     /locus_tag="VFY80_03255"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6006951.1"
                     /translation="VAPMKLPLDVDEIGVAGGLAGEPIRVVHGKTVDLLVPAEAEIVI
                     EGMIDTEYLEPEGPFGESHGHIALEQFNMPMRVTAITHRRNAIVASYLSQVTPSESSA
                     IKRVSYEPMFLAHLRNTLGIRAVKRVKLHERLTAVRRVIVITVEKGAPRSEIWRALYG
                     ASSFKADCGKICIAVNEDIDPDNSDAIFWALAFRMDPANDLQVLPHRSGGHGPEREHE
                     SDHEDGTLLMDATVKEELPPLALPKQAYMERARKIWEELGLPKLHPQSPWCSAPTGDW
                     TPQWDEAAKRAAEGRYLENGEESAKRRRKGLKPETTCRPDED"
BASE COUNT          375 a          727 c          689 g          386 t
ORIGIN      
        1 gccgcaccgg aatttccgac gatctccgaa gcgggcgtcc cggcctacca ccatcagtcg
       61 tgggtcggca tgctggcgcc ggctaagacg ccgcggccga tcatcacgcg actcaacagc
      121 gaagtcgtca ggatcgtgca ggccccggat atgaagtcgc tgctcctgcg tgacgggctc
      181 gaggcggcgg gagacaagcc ggaggagttc gagaaggaca tcaaggaaga gatcgcgaag
      241 tggcagaaac tcaccaaggc ggccggaatc aaggccgaat gagcagcgat cgtctttccg
      301 gcaaatgccg aggcccggaa ttttttgaag cccggtttac tggataagca gaccccgata
      361 tggacgagct tgcgagattc tgtgagctcg tgagagcagc gcgaaatggc gttgccttca
      421 ccggcgcagg gatcagcacc gagagcggca taccggactt tcgcggtccg cagggcgtgt
      481 ggaccaccga gacgcctgtc ctgtaccagg atttcatggc ggaccgcgcc gcacgcgtgc
      541 gcgcttggga gcgcggtgtg cgcatgttcc atcgctgcaa gagcgcccgc cccaacgagg
      601 gccatctcgc gctcgcgcaa ctcgagcggc gcgggcatat cgccgccgta atcacccaga
      661 acatcgatgg cctgcatcgc gacgccggca gcaccgaagt cgtcgagctg cacggcacga
      721 accgttacag cgcctgccag cactgcggaa aagagtggac gaccgcccag atcgtggcgc
      781 gggtggagcg cggtgaagag gcgccggaat gcgacgattg ccgcgggcca atcaagacgc
      841 gcacgatctc gttcggacaa tcgatgccgg agacggaaat gcgccgcgcc gccgagctct
      901 ccgtcgccgc tgatctcttc gtctcgatcg gctcctcgct cgtcgtcgag cccgcagcct
      961 ctttcccgcg gctcgccagg cactccggcg cgaagctcgt catcctgaac aaccaggaga
     1021 cgccgctcga tgatctcgct gatctcgtga tccgcgagag catcggcgtc accctgcgca
     1081 cactccttgc gcggctgtag ggtcgtgaca ccggcgaaag ccgatgtcca gagagcgcgc
     1141 ttcacacggc ttgatgccgc ttatgccgac ttagcgagaa agctatgcgg gcgccctcag
     1201 ccctcggcgc gaaagccggt tcagtcctcg tcgggccggc aggtggtttc gggtttgagg
     1261 cccttgcgcc gccgtttcgc gctctcttcg ccgttttcca gatagcggcc ctcggccgcg
     1321 cgcttcgccg cctcgtccca ttgcggcgtc cagtcgcccg tcggcgcgct gcaccacggt
     1381 gactgcggat gcagtttcgg caggcccagc tcctcccata ttttccgcgc gcgctccatg
     1441 tatgcctgct tcggcagcgc gagcggaggc aactcctcct tcaccgtcgc gtccatgagg
     1501 agggttccat cttcgtgatc ggactcgtgc tcgcgctccg gcccgtggcc gccgcttcgg
     1561 tgcggcagca cctgcagatc gttcgccgga tccatgcgaa acgcgagcgc ccagaagatc
     1621 gcgtcggaat tgtccggatc gatgtcttcg ttgaccgcga tgcagatctt gccgcagtcc
     1681 gccttgaagc tggaggcgcc gtagagcgcg cgccagatct ccgagcgcgg cgcgcccttc
     1741 tccacggtga tgacgatgac gcgccgcacg gcggtcaacc gctcgtggag cttcacgcgc
     1801 ttcaccgcac ggatgccgag cgtgtttcgc agatgcgcaa ggaacatcgg ctcgtacgac
     1861 acgcgcttga tcgcgctcga ctcgctcggc gtcacctggc tcaagtacga cgcgacgatc
     1921 gcgttcctgc ggtgcgtgat tgcggtgact cgcatcggca tgttgaactg ttcgagcgcg
     1981 atatggccgt gggattcacc gaatggtccc tcaggctcca ggtattcggt gtcgatcatg
     2041 ccttcgatca cgatctccgc ttccgcaggc accaggagat cgaccgtctt gccgtgcacg
     2101 acgcggatcg gttcgcccgc cagaccgccc gcaacgccga tctcatccac gtcgagcggc
     2161 agcttcatcg gcgccac
//
LOCUS       DASURG010000194         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_545708, whole genome shotgun sequence.
ACCESSION   DASURG010000194 DASURG010000000
VERSION     DASURG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545708"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..499)
                     /locus_tag="VFY80_03260"
     CDS             complement(20..499)
                     /locus_tag="VFY80_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6006952.1"
                     /translation="MAGLRSAFIAEAGSDRVNARRMAHRLAAGMVVLRRFDGEWRCLM
                     LRAYRLWDFPKGMLEPGEDPLAAARREVREETTLEALAMPWGDIYSETDPYGGKVARY
                     YLAESREGPVSLPVSAELGRPEHHEFRWVSLDEARALARPRLKRVIEWAAQHANTAR"
     gene            complement(448..1020)
                     /locus_tag="VFY80_03265"
     CDS             complement(448..1020)
                     /locus_tag="VFY80_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006953.1"
                     /translation="MASIIAGNFENNARATEAVEELLHSGLVPDQVCVFFLNPPGQHA
                     THPVGGDREESPGARESDTGALKGAALGGAVGLGAGAAAAAGTAALGPIAALAALGVG
                     AYTGSLVGALSKSGKGPEREAGNRPKPEPRRAGMLVAAHIADSVPEARLIDVLKKYGA
                     RDIERAEGMWRDGRWQDFDPLSSPKRVATA"
     gene            complement(1104..>2077)
                     /locus_tag="VFY80_03270"
     CDS             complement(1104..>2077)
                     /locus_tag="VFY80_03270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX6006954.1"
                     /translation="VPFDLSRMMFICTANVLDTIPGPLRDRMEVIQLPGYTEQEKLEI
                     ARRYLVGRQLAANGLTAAQLEIGDDALAAIIRDYTREAGVRNLEREIGAVCRNVAVRI
                     AEGAVQNVRIGVDDLAPILGPRRFESEVAMRTSIPGVATGLAWTPVGGDILFVEATRM
                     PGSGKLILTGQLGEVMKESAQAALSLVKARARTLGVKEEALEKSDIHVHVPAGATPKD
                     GPSAGVAMFVALSSLLTARAVKSEVAMTGEISLRGLVLPIGGVKEKVLAALRAGITTV
                     MLPARNRKELDEIPQEAREKLTLVWIETVDDALATALGAAPAAAVPA"
BASE COUNT          321 a          736 c          663 g          357 t
ORIGIN      
        1 cgcggatgtt ttgctcggat cagcgcgcgg tgttcgcgtg ctgcgcagcc cactcgatca
       61 cccgcttgag gcgaggccgg gcgagcgcgc gtgcttcatc cagactcacc cagcgaaatt
      121 cgtgatgttc cgggcggccg agctccgcac tgaccggtag ggaaaccggc ccctcgcgcg
      181 actccgccag gtaatagcgt gccactttgc cgccgtacgg atccgtttcg gaatagatgt
      241 cgccccacgg catggcgagc gcttcgagcg tggtctcctc cctgacttca cgccgtgcgg
      301 cagccagcgg gtcttcaccc ggctcgagca tgcccttggg gaaatcccac agccggtacg
      361 cgcgcagcat aagacagcgc cattcaccgt cgaagcgtcg cagcaccacc atgcctgccg
      421 ccagccggtg cgccatgcgg cgggcgttta cgcggtcgct acccgcttcg gcgatgaaag
      481 cggatcgaag tcctgccatc tgccgtcgcg ccacatgccc tcggcgcgct cgatatcgcg
      541 cgcaccatac ttcttcagca cgtcgatcag ccgcgcttcc ggcacgctgt ccgcgatatg
      601 ggccgccacc agcatcccgg cccggcgcgg ttcgggcttg gggcgattcc cggcctcgcg
      661 ctccggccct tttccgctct tgctcagtgc gccgacgagc gatccggtat aggcccccac
      721 gccgagcgcc gccagtgcag caatcggacc gagcgctgcg gtgcccgcgg cagcggccgc
      781 gcccgcgcca agtcccacgg caccccccaa ggcagcgccc ttcagcgcgc cggtatcgct
      841 ctcgcgcgcg ccgggcgact cctcacgatc accgcccacg ggatgggtcg catgctgacc
      901 cgggggattc agaaagaata cgcacacctg atcgggcaca agaccggaat gcagcagctc
      961 ctcgaccgct tcggtcgcgc gcgcgttgtt ctcgaaattg cctgcaatga tgctcgccat
     1021 gacgggatct ccttgagcgt gaccccctgt aagacagcgc gtaccgccgg tggatcgatg
     1081 agttttttta aaaccgcggc gggttatgcc ggaactgccg cggccggcgc cgcgccgagc
     1141 gcagtcgcga gcgcgtcatc cacggtttcg atccacacga gcgtgagctt ttcccgcgct
     1201 tcctgcggaa tctcgtcgag ttccttgcga ttgcgcgcag gcagcatcac ggtggtgatt
     1261 cccgcgcgca gcgccgcaag caccttctcc ttgacgccgc cgatcggcag cacgagcccg
     1321 cgcaggctga tttcgcccgt cattgcgact tcgctcttca ccgcgcgggc agtgagcagc
     1381 gaggagagcg cgacgaacat cgcgactccc gcgctcgggc cgtccttcgg cgtggcgccc
     1441 gcgggaacgt ggacgtggat gtcggatttc tcgagcgctt cctctttcac gccgagcgtg
     1501 cgcgcgcgcg ccttgacgag ggaaagcgcc gcttgcgcgc tttccttcat gacctcgccg
     1561 agctggccgg tgaggatcaa cttgccgctg cccggcatcc gcgtcgcctc cacgaagaga
     1621 atgtcgccgc cgacgggggt ccacgcgagg ccggtggcga cgcccggaat gctggtgcgc
     1681 atggcgacct cggactcgaa gcggcgtggg cccaagatcg gtgcgagatc gtcgacgccg
     1741 atacgcacgt tctgcacggc accttccgcg atgcgcaccg caacgttgcg gcagacggcg
     1801 ccgatctcgc gctcgagatt gcgcacgccc gcttcacgcg tgtagtcgcg gatgatcgcg
     1861 gcaagcgcgt cgtccccgat ttcgagctgc gcggcggtga ggccgtttgc cgcgagttgc
     1921 cgacccacga gatagcgccg cgcaatctcg agcttctcct gctcggtgta tcccggaagc
     1981 tggatcacct ccatgcggtc gcgcagcggt ccgggtatgg tgtcgagcac gttcgccgtg
     2041 cagataaaca tcatgcgcga gagatcgaac ggcaccg
//
LOCUS       DASURG010000195         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_955210, whole genome shotgun sequence.
ACCESSION   DASURG010000195 DASURG010000000
VERSION     DASURG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955210"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFY80_03275"
     CDS             complement(<1..954)
                     /locus_tag="VFY80_03275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6006955.1"
                     /translation="MLRFAVSKETLTWHMQDVVVSVVVPTRARPVLLARCLKTLCSQT
                     LAPAHYEIIIVDDGPDRETRRTVETCAASLAGRGPFLHYVASNGPHGPAAARNRGWKL
                     ARGGIVAFTDDDTEPDANWLAAGLAAFDGETDAVSGRIVVPLPPAPTDYEFDVAGLAR
                     AEFATANVFCRKSVLTRIEGFDERFPLAWREDSDLQFRLLRAGASIRKAGDAVVVHPV
                     RAAGWGVSLRQQKKVMFDVLLYREHPRLYRDRIRRAPRWDYYITVAALLIGAAALALG
                     ADSVVIAAAAVWTSMTVWLCAKRLRHSSRRLRHIAEIMVTSV"
     gene            complement(1067..2449)
                     /locus_tag="VFY80_03280"
     CDS             complement(1067..2449)
                     /locus_tag="VFY80_03280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX6006956.1"
                     /translation="MQICLIGTGYVGLVAGACFAEMGHVVTCVDCNAAKVARLERGVV
                     GIYEPGLPALVSRNVARGRLRFTTDLARAAMVPLHFICVGTPSHSDGSPDITQVMAAA
                     RQLGRTLADRATVVVRSTVPVGSTERVEAALLAEVGRRGGDIAIDTGFSPEFLREGKA
                     VDDFMHPDRVIIGSRSPRVIALLRRLYIPLLDDAERLIVMRTRDAELAKQAANAMLAT
                     RISFMNEIAGICERLGADAESVRIGIGSDPRIGGAFLQPGCGYGGSCLPKDTRALIRV
                     AYEAGFDPALLNAVDLRNRRQKTVLFEKIRRRFGPALRGLRFAIWGLAFKPDTDDMRE
                     APSLALVQALLEAGALVSAHDPEAMANARRVLPHVWIESGRLNFTGQYDAIENADALV
                     LVTEWAVFRSPDLARLARSMRQKVIFDGRNQYDGLALRNAGFEYFGVGRAAPALAPAA
                     PDLRLASSRA"
     gene            complement(2503..>3005)
                     /locus_tag="VFY80_03285"
     CDS             complement(2503..>3005)
                     /locus_tag="VFY80_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006957.1"
                     /translation="TALYAVALANVVLAVSAGLLAYRSLKTPFFPYTSYEIDRLRHGD
                     LARPPEGAPTEALAALRDPGPRERALLRSEAELEARLTEVRYATPQLIATPSVIAAVA
                     GAGLVVGLLTSQGKSRAHREPMHAAHVPLTRQLLNIAFGQLSSLAVAAAMREFQRRAG
                     HHGPPY"
BASE COUNT          554 a          984 c          994 g          473 t
ORIGIN      
        1 caccgaggtc accatgattt ccgcgatatg ccgcagcctg cgcgagctgt ggcgcaggcg
       61 cttcgcgcag agccacactg tcatcgaggt ccacacggcg gcagccgcga tgacaacaga
      121 gtctgcaccc agggcaagcg cggctgcacc gatgagcagt gcggccaccg tgatgtaata
      181 gtcccagcgc ggggcgcgcc gtatgcggtc gcggtagagc cgcgggtgct cacggtagag
      241 caggacgtcg aacatcactt tcttctgctg cctcaagctc acgccccatc ccgccgcacg
      301 aaccggatgc acgacgaccg catcgcccgc cttgcggatt gaggcgcccg cgcggagcag
      361 gcggaactgc agatcggagt cctcgcgcca ggccagcgga aagcgctcat cgaaaccttc
      421 gatgcgggtg agcacggatt tccggcagaa gacattcgca gtggcgaact ccgcgcgggc
      481 gagtccggca acgtcaaact cgtaatcggt gggtgcaggg ggaagcggaa ctacgatgcg
      541 gccggaaacc gcatcggttt ccccatcgaa tgcggcgagg cctgccgcga gccagttggc
      601 gtctggttcg gtatcgtcgt cagtgaacgc cacgatgccc ccacgggcga gcttccagcc
      661 acgattgcgc gccgctgcgg gcccgtgcgg accgttggag gcaacataat gaagaaacgg
      721 tccacgaccg gcgaggcttg cggcacaggt ctctaccgtt cggcgtgtct cgcgatccgg
      781 cccgtcgtcc acgatgatga tctcgtagtg cgcaggcgcc agggtctgac tgcacagcgt
      841 ttttaagcag cgcgccagga gcacgggccg cgcgcgtgtg gggacgacta ccgatacgac
      901 aacgtcctgc atgtgccacg tcaacgtctc tttggagaca gcgaagcgca gcaagtctta
      961 ttccaggaac ggtatgccag cggtagagcc cagtcgaggc cggagccaaa cggtaaatct
     1021 tgcgcggcga cccgccaatt tttccgccac ccggcggtcc gacgctttag gcgcggctcg
     1081 aggcgaggcg caagtccggt gcggcaggag cgagcgccgg tgcggcgcgg ccgacgccaa
     1141 agtactcgaa gcctgcgttg cggagggcga ggccgtcata ttgattgcga ccgtcgaaga
     1201 tcactttctg tcgcatgctt cgagcgagcc gcgcaagatc gggagaccga aacactgccc
     1261 actccgtaac cagcaccagc gcatctgcgt tctcgatcgc gtcgtactgg cccgtgaaat
     1321 tgagccggcc gctttcgatc catacgtgcg gcagcacacg acgcgcattc gccatcgcct
     1381 cgggatcatg cgcgctgacc agggcacccg cctcgaggag cgcctgcacc aatgcgagcg
     1441 aaggcgcctc gcgcatgtcg tccgtatcgg gcttgaaggc aaggccccag atcgcgaatc
     1501 gcagcccgcg cagcgcaggt ccgaaacgtc gccggatctt ttcgaaaaga acggtcttct
     1561 gcctgcggtt gcgcagatcg accgcattca gcagcgcggg gtcgaaaccc gcctcgtaag
     1621 cgacgcgaat gagcgctcgc gtatccttgg gcaggcacga gccgccgtag ccgcatcccg
     1681 gctggaggaa cgcgccaccg atgcggggat cgcttccgat accgatgcgc acgctttccg
     1741 catcggcccc gaggcgctcg caaatgcctg cgatttcgtt catgaacgaa atgcgcgtcg
     1801 ccagcatcgc atttgccgcc tgcttcgcga gctccgcatc gcgtgtgcgc atgacgatga
     1861 gccgctccgc gtcatccagc agcggaatgt agagccggcg gagcagcgcg atgacgcggg
     1921 gactgcggct gccgatgatg acgcggtcag gatgcataaa gtcatcgacg gccttgccct
     1981 cgcgcagaaa ttcgggactg aagcccgtgt cgatcgctat gtcgccgccg cgcctgccca
     2041 cctccgcgag cagcgccgcc tccacgcgtt ccgtggaacc gaccggaacg gttgagcgca
     2101 cgacgaccgt ggcgcgatcg gcgagggtgc ggccgagctg gcgcgccgcc gccatgacct
     2161 gtgtaatgtc cggtgagccg tcgctgtgtg aaggggtccc gacacagatg aagtgaagcg
     2221 gaaccatcgc ggcgcgggca aggtcggtgg tgaagcgcag tcgcccgcgt gcgacgttgc
     2281 ggctcacgag ggcaggaagt cccggctcgt atatcccgac gactcctctc tcgagccgtg
     2341 ccaccttcgc cgcgttgcag tccacacagg tgaccacgtg gcccatctcg gcgaaacacg
     2401 cgcctgcaac aagtccgacg tagccggtgc cgatgaggca gatctgcatg tgccgctcag
     2461 ccgatgcggg tcatcgccga gcttcgttgc agcgcaagct cctcagtacg gcggcccatg
     2521 atgtcccgcc cggcgctgga attcgcgcat cgcggccgcg actgccaggc tcgacagctg
     2581 accgaacgcg atattgagca attggcgggt cagcggcacg tgtgctgcgt gcatcggctc
     2641 acgatgcgcg cgcgatttcc cttgcgaagt gagaagcccc acaacgaggc ccgcgccagc
     2701 aaccgccgca atgacggaag gggtcgcgat gagttgcggc gtcgcgtacc gcacctcggt
     2761 cagacgggct tcaagttccg cctccgagcg caacaaggcg cgctcccgcg ggccgggatc
     2821 cctgagcgcc gcgagagctt ccgtcggcgc gccttctggc ggacgcgcca gatcgccgtg
     2881 ccgcagccga tcgatctcgt aagaggtgta gggaaagaac ggggtcttga gcgaccggta
     2941 cgccaggagc ccggcactca cggcgagaac gacgttcgcg agggcgacgg cgtagagcgc
     3001 cgttt
//
LOCUS       DASURG010000196         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_955425, whole genome shotgun sequence.
ACCESSION   DASURG010000196 DASURG010000000
VERSION     DASURG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955425"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFY80_03290"
     CDS             complement(<1..621)
                     /locus_tag="VFY80_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX6006958.1"
                     /translation="MPANARQADALRLIVETYLAHHGEETGVGASADRVQVVVHIDHE
                     ILSEARAAHADGPHVCEIEDGPALALDTARRLACDATVVGLVEGEDGEPLDIGRKTRS
                     IPAAIARALKARDGGCRYPGCDRTRFTEGHHIEHWADGGETKLGNLVTLCSFHHQLVH
                     EGGFGLTMTDDGAFVFTRPDGRRVEENGARRFRGNVSLPGHPSYPGA"
     gene            complement(659..1888)
                     /locus_tag="VFY80_03295"
     CDS             complement(659..1888)
                     /locus_tag="VFY80_03295"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX6006959.1"
                     /translation="MEIIIERCAGLDVHKQAVVVSVRMPGEGGKRRVETQTFSSMSEE
                     LLKLLDWLSSHGVTHVAMESTGVYWRPVYALLEDEFEVLLVNARHVKNVPGRKTDVRD
                     SEWLAQLLECGLLKGSFIPPRPIRDLRDLTRYRKVLIRERGHHVNRVEKTLELTNIKI
                     SSVVTDIMGKTGRAILQALIAGETDPDRLADLAQGLLKNKKAALREAVKGKITPHYAF
                     LLEQQLRIIDELTRRIEEFDARIEASMRPFDEAYRCAMSMPGIAERSAQAILAEIGTD
                     MSRFPTPGNLVSWARVCPGNNESAGKRRSSATGKANGWLRATLNEAAWAAARTKNSYY
                     RALYQRMKARQGPKKAIGAVQHAMLRALWYMLSRHVLHQDLGADYFTRNNVARVSRHH
                     VRRLEQLGYRVTLEEAA"
     gene            1974..2327
                     /locus_tag="VFY80_03300"
     CDS             1974..2327
                     /locus_tag="VFY80_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006960.1"
                     /translation="MRINTTGYLEALESGSASGSSHQKHNRPLGHRQFPPFGAWRATW
                     WFRGNVSLPGHPSYPGAGDDLRIRTLNREAGLRIDAHTSRCRWQGETMDYSMAIEGMY
                     GLEEHAARASSAQPA"
     gene            complement(2332..>2871)
                     /locus_tag="VFY80_03305"
     CDS             complement(2332..>2871)
                     /locus_tag="VFY80_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6006961.1"
                     /translation="GAEAMLRSDHAAALAYLREAARVEADIAGIWVNLGVLYSRHGQY
                     EHAEAAYLRALEAEDGEESALANLALVYQALGEIELAAEYRERVQSYRERNPYYHYTK
                     AARAYEEQQFDAALASLRKALRLKRDEHAFYSLRGQVLTALGRQKDATQSFARAREYA
                     DAAELRARSRVAFQAPPLQ"
BASE COUNT          487 a          946 c          868 g          570 t
ORIGIN      
        1 ggcgccggga tagctcgggt gcccgggtaa ggaaacgttt ccgcggaaac gtctcgcgcc
       61 gttctcctcg acgcgccgcc cgtcgggtcg ggtgaacacg aacgcgccgt cgtcggtcat
      121 cgtgagcccg aatccgccct cgtgaacgag ctggtgatgg aaactgcaca gcgtgacgag
      181 gttgccgagc ttcgtctcac cgccatcggc ccagtgctcg atgtggtgac cctcggtgaa
      241 gcgcgtccgg tcgcagcccg gataccggca gccgccgtcg cgcgccttga gcgcccgtgc
      301 gatggctgcg ggaatgctgc gcgtcttgcg gccgatgtcg agcggctcgc cgtcttctcc
      361 ttcgacgagc ccgacgaccg ttgcgtcgca ggcgagccgc cgcgccgtgt cgagcgcgag
      421 cgccgggccg tcctcgatct cgcacacatg cggcccgtcc gcgtgcgccg cccgcgcctc
      481 ggataggatc tcgtgatcga tgtggacgac gacttgcacg cggtcggccg aggcgcctac
      541 cccggtctcc tcgccgtgat gggcgaggta ggtttcgacg atgagccgta gcgcgtcggc
      601 ttgacgcgcg ttggccggga tctgccgaaa cgtttcctat gaaacagctg ccccttcgct
      661 aggccgcctc ttcgagtgtc actcgatagc caagttgctc gagacggcgg acgtgatggc
      721 ggcttacgcg agcgacatta ttgcgcgtga agtagtcggc gcctaggtcc tgatgcagga
      781 catgccggga gagcatgtac cagagggcgc ggagcatcgc gtgttgcacg gcaccgatcg
      841 ctttcttcgg gccctgtcgg gccttcatac gttggtagag ggcgcggtag tagctgtttt
      901 tcgttcgagc cgccgcccaa gccgcttcat tcaacgtcgc acgtagccag ccattggctt
      961 tgcctgttgc cgatgaacgc cgcttaccgg cgctttcgtt gttgcctgga cagacccgag
     1021 cccaagagac cagatttccc ggcgtcggga agcgcgacat atcggtgcca atctccgcga
     1081 gaatcgcctg tgctgatcgt tcggcgatcc caggcatgga catggcacac cgataggctt
     1141 catcgaaagg gcgcattgaa gcctcgatgc gagcgtcaaa ctcctcaatt cgacgagtga
     1201 gttcatcgat gattcgcagc tgctgctcaa gcaagaacgc atagtgaggg gtgattttgc
     1261 ctttgactgc ttcccgcagt gccgctttct tgttcttcag caacccttgc gcaagatctg
     1321 ccagtcgatc cggatcggtt tctcctgcga tgagtgcctg caagatcgca cgacctgttt
     1381 tgcccatgat gtcggtaacc accgagctga ttttgatgtt cgtcaactcc agcgtcttct
     1441 cgacccgatt gacgtgatga ccgcgctctc ggatcagcac tttgcggtat cgcgttagat
     1501 cacgtagatc gcgaatcggc cgcggcggaa tgaagcttcc cttgagtaag ccgcactcca
     1561 gcagctgtgc cagccattcg ctgtcacgta catcggtttt gcgtcccgga acgttcttca
     1621 cgtgtcgcgc gttgaccaga agaacctcga actcgtcttc caacagtgcg tataccggtc
     1681 gccagtacac tcccgtcgat tccatggcta cgtgcgttac accatggctc gacaaccaat
     1741 ccaaaagctt taagagctcc tccgacatgc tcgagaacgt ctgcgtctcg actctccgtt
     1801 tgccgccttc acctggcatc cgcacgctca ctacaaccgc ctgcttgtgt acgtcgaggc
     1861 ccgcacaccg ctcgataatg atctccatgc gccaatcctc cctgcgtttt caattacgct
     1921 ggccggagcg cttattaaga gaagagtttc ctgatacgtg attgcgcacc accgtgcgca
     1981 tcaacactac ggggtacctc gaagcgctcg agtcaggttc cgcgtcgggc tcttcgcacc
     2041 agaaacataa ccgacctctc ggccaccgcc agtttccgcc cttcggggcg tggcgcgcca
     2101 catggtggtt ccgcggaaac gtttccttac ccgggcaccc gagctatccc ggcgccggcg
     2161 acgatctgcg aatccgcacg ctgaaccgag aagccgggct ccgcatcgac gctcacacga
     2221 gccgctgccg ctggcagggc gagacgatgg actacagcat ggcgatcgag ggcatgtacg
     2281 gcctcgagga acacgcggcg cgtgcatcct cggcccagcc ggcctgacgc gctactgcag
     2341 aggcggcgcc tgaaacgcga cgcgcgagcg cgcccgcagt tccgccgcgt cggcatattc
     2401 ccgcgcacga gcgaagcttt gcgtcgcgtc cttctgccgg ccgagcgccg tcagcacttg
     2461 gccgcgcagc gagtagaacg catgctcgtc gcgcttcaag cgcagcgcct tgcgcagcga
     2521 cgcgagcgcg gcgtcgaact gttgctcctc gtaggcgcgg gccgccttcg tgtagtggta
     2581 gtagggattc cgctcacgat agctctgcac acgctcgcgg tactcggccg cgagctcgat
     2641 ctcgccgagc gcctggtaca cgagcgcgag attcgcgagc gccgattctt ccccatcctc
     2701 cgcttcgagc gcgcgaagat aggcggcttc cgcatgctcg tactgcccgt ggcgcgagta
     2761 cagcacgccc aagttcaccc agatgccggc gatgtcggct tcgactcggg cggcctcccg
     2821 caaataagcg agcgcggcgg catggtcgct gcgcagcatg gcttcggccc c
//
LOCUS       DASURG010000197         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1363579, whole genome shotgun sequence.
ACCESSION   DASURG010000197 DASURG010000000
VERSION     DASURG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363579"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFY80_03310"
     CDS             complement(<1..288)
                     /locus_tag="VFY80_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006962.1"
                     /translation="MARLPRRRLAGFYFFYFAYLGSFAPFFSLYLEAAGMTAVEIGLL
                     MALPQVTRIIAPHLWGWLADRGPSRLRIVRLTGLAGLVCFLGVFASTAFALL"
     gene            complement(297..1460)
                     /gene="aroC"
                     /locus_tag="VFY80_03315"
     CDS             complement(297..1460)
                     /gene="aroC"
                     /locus_tag="VFY80_03315"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX6006963.1"
                     /translation="MSGNTLGRLFCVTSFGESHGPAIGCVVDGCPPGMTLSEADIQPE
                     LDRRKPGTSRHVTQRREEDVVEILSGVFEGVTTGTPIALLIRNVDARSKDYSKIKDMF
                     RPGHADYTYWQKYGVRDYRGGGRQSARETAVRVAAGAIAKKWLRERYGVMVRGHLAQL
                     GPIVIPFKSWDGVYSNPFFVADESYVPKLEEFMDKLRKSGDSAGARITTIASNVPVGL
                     GEPVYDKLDADLAYNMMSINAVKGVEVGAGFAVVAQKGTEHSDEMTPEGFLSNNAGGI
                     LGGISTGQDIVVHVAVKPTSSIRLPRRTIDREGHPTIIETHGRHDPCVGIRATPICEA
                     MMALTLMDHALRHRAQNADVRCATPRIPSRAPQSVIEKLRAAAPTEDPEPDEA"
     gene            1549..1737
                     /locus_tag="VFY80_03320"
     CDS             1549..1737
                     /locus_tag="VFY80_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006964.1"
                     /translation="MTEKTGSDKAKKPRGFTVRELHQKVKQNHLHNQQAGRTRNALAQ
                     PQNRWQRFARYVWDKKKG"
     gene            complement(1757..>2008)
                     /locus_tag="VFY80_03325"
     CDS             complement(1757..>2008)
                     /locus_tag="VFY80_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HEX6006965.1"
                     /translation="GFPIQSMLPGYGSEQAFTAKQAKLGAPIALAGEKQLRAPIDYKR
                     WVLWASLILGVAVVGLMAYRLLRQMPSAPGARPPPDSRA"
BASE COUNT          381 a          644 c          640 g          343 t
ORIGIN      
        1 gagcagcgcg aaggccgtac tcgcgaagac cccgagaaag cacacgagcc ccgcgagtcc
       61 ggtgagccgc acgatgcgaa ggcggcttgg cccacggtcg gcgagccagc cccagagatg
      121 cggcgcgatg atgcgcgtca cctgcggcag ggccatgagc aggccgattt cgaccgccgt
      181 catgccggcc gcctcgaggt agaggctgaa aaacggcgcg aaggagccta agtacgcgaa
      241 gtagaaaaaa taaaaaccgg caagacgccg gcgcggaagg cgagccatgc gaggcctcag
      301 gcctcgtccg gctcggggtc ctcggtgggc gcggctgcgc gcagtttttc gatcaccgac
      361 tgcggcgccc gggaaggaat ccgcggcgta gcacagcgca cgtctgcgtt ctgtgcgcga
      421 tggcgcagcg cgtggtccat cagcgtgagt gccatcatgg cctcgcagat cggcgtcgcg
      481 cggatcccga cacacggatc gtgccggccg tgcgtctcga tgatcgtcgg atgcccctcc
      541 ctgtcgatcg ttcggcgcgg aagccggatg ctcgaggtgg gcttgaccgc aacgtgcacg
      601 acgatgtcct gaccggtcga gatgccgccc aggatgccac ccgcgttgtt cgagagaaag
      661 ccttccggcg tcatctcgtc cgagtgctct gtccctttct gcgcaaccac ggcgaagccc
      721 gcgccgacct ccacgccctt cacggcattg atgctcatca tgttgtaggc gagatcggca
      781 tcgagtttgt cgtagaccgg ctcccccagg ccaaccggca cgttggatgc gatcgtcgtg
      841 attcgcgctc cggcggaatc accggacttg cggagcttgt ccatgaattc ttcgagtttt
      901 ggcacgtagc tttcatcggc gacgaagaaa ggattcgaat acacgccatc ccacgacttg
      961 aacggaatca cgatgggccc aagctgagcg agatgaccgc gcaccattac gccgtatctt
     1021 tcgcgcagcc acttcttcgc aatcgcgccc gccgcgaccc gcacggccgt ctcgcgtgcg
     1081 gactgacggc cgccgccgcg atagtctcga acgccgtatt tctgccagta agtgtaatcg
     1141 gcatgtcccg gccggaacat gtccttgatc ttcgaatagt ccttgctgcg cgcatcgacg
     1201 ttgcggatga ggagcgcgat cggcgtgccg gtggtcacgc cctcgaaaac accggagagg
     1261 atttcgacga cgtcttcttc ccgtcgctgc gtcacgtgac gcgacgttcc cggcttgcgg
     1321 cgatcgagtt cgggctgaat gtcggcttcg gagagcgtca tgccgggcgg gcagccatcg
     1381 acgacgcaac cgatcgcggg accatggctt tccccgaacg aggtcacgca gaagagcctg
     1441 cccaacgtgt tgcccgacat gcctgtcctg ttggtcgaag gaagaaaccc ggcgcatccc
     1501 gcgcagtctc agtttaacat aggctgctcg acgatccgga cgcattccat gaccgagaaa
     1561 actggctctg acaaggcgaa aaagccccgc gggttcaccg tgcgcgaact gcaccagaaa
     1621 gtgaagcaga accatctgca taaccagcag gcaggcagga cgcgcaacgc cctcgcgcag
     1681 ccgcagaatc gctggcagcg cttcgcgcgt tacgtctggg acaagaagaa aggctagcga
     1741 ctctgcggtg cccgtctcac gcgcggctgt ccggaggcgg gcgtgcgcct ggcgcgctcg
     1801 gcatctggcg cagtagccga taggccatca agccgacgac tgcaacgccg aggatgaggc
     1861 tcgcccacag cacccagcgc ttgtagtcga ttggcgcgcg cagttgtttt tctccggcga
     1921 gcgcgatcgg ggcgccgagc ttcgcctgtt tcgccgtaaa cgcctgttcc gagccgtatc
     1981 ccggcagcat ggactggatg ggaaagcc
//
LOCUS       DASURG010000198         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_36150, whole genome shotgun sequence.
ACCESSION   DASURG010000198 DASURG010000000
VERSION     DASURG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36150"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFY80_03330"
     CDS             complement(<1..825)
                     /locus_tag="VFY80_03330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6006966.1"
                     /translation="MRRLKVLTWHTHGSYLYYLTQAPHEFYVLSKPERPPGYTGRWGH
                     LPWGANVHDLPVSKAAMQTLDCILFQDDHQYLHDQYEFLSEAQRHLPRIYLEHDPPCA
                     HPTDTRHPVDDPAMLLVHVTPFNQLMWDSGRTPTLVIEHGVIVPQGVRYRGDVARGLV
                     IVNNLGRRGRRLGSDVFDYVRRRVPLDLVGMGAHDCGGLGEIRHGELPQFAAQYRFLF
                     NPIRYTSLGLAVIEAMMVGLPVVALATTEMATVIRSGVNGYADTRVDTLIEHMSRLL"
     gene            complement(813..1928)
                     /locus_tag="VFY80_03335"
     CDS             complement(813..1928)
                     /locus_tag="VFY80_03335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX6006967.1"
                     /translation="MPEEAEKVRAMVRDLPGAPRTILVIRALQLGDLLCAVPALRALR
                     AAFPTARVTLAGLPWAREFAARFRRYVDDFIEFPGFPGLPERVCDVGNVPRFLEAVQA
                     RRFDLALQMHGDGRLLNPLTALVNARHYAGYCAEGAYCPDRERFLPWRQDEHEVLRYT
                     RLMRALGVPVGDDRLEFPITAQERSDCGQLAAEHGLERGSYVCVHPGSQLPSRRWPAA
                     RFAAVGDRLAEDGYKIVLTGTSGEAGLVQDVAATMHHIPVNLVGGTTLGVLAALVEAA
                     ALIVCNDTGVSHIAAAVGTPSVVVCCGSDFRRWAPLDRDRHCVLHHPVGCRPCAYLQC
                     PIGHPCATGVGIDEVTIEARRLIGSYRPFDRRAVCVG"
     gene            complement(1921..>2511)
                     /locus_tag="VFY80_03340"
     CDS             complement(1921..>2511)
                     /locus_tag="VFY80_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6006968.1"
                     /translation="DIRALLEYSKLPYRRAHDVAEETADILAADRIVGWFQGRMEFGP
                     RALGARSILASPLTAEIQQRLNAIKDREDFRPVAPVVLEEEAGKWFAGAQVSPFMLFV
                     HDVVRERRHRIPGVCHVDGTARIQTVNAAQHALYHRLIKAFQRRTDVPVLVNTSFNTQ
                     GEPIVCTPQDAVRCFMTSPLDALVIGSYILEKPQRA"
BASE COUNT          456 a          837 c          824 g          394 t
ORIGIN      
        1 caggagccgg gacatgtgct cgatcagcgt atcgacgcgc gtgtccgcat agccgttcac
       61 gccgctccgg atcacggtcg ccatctcggt ggtcgccagt gcaacgaccg gcagcccgac
      121 catcatcgcc tcgatcacgg cgagtcccag actcgtgtag cggatcggat tgaacaggaa
      181 ccggtactgc gccgcaaact gcggcagctc gccgtgccgg atttcgccca ggccgccgca
      241 gtcgtgtgcc cccatgccga ccagatcgag cggtacacgg cgtctcacgt aatcgaagac
      301 gtccgagccg agacgccgtc cgcggcgtcc gaggttgttc acgatgacga ggccacgcgc
      361 aacatcgccg cgatagcgca cgccctgggg aacgatgacg ccgtgctcga tgaccagcgt
      421 cggcgtgcgg ccgctgtccc acatgagctg gttgaacggc gtcacgtgca ccagcagcat
      481 cgcgggatcg tccactggat gccgggtatc ggtgggatgc gcgcacggcg gatcgtgctc
      541 gagatagatg cgcggcaggt gccgttgcgc ttcgctcagg aattcgtatt ggtcgtgcag
      601 gtactggtgg tcgtcctgaa acaggatgca gtcgagcgtt tgcatcgccg ccttcgagac
      661 cggcagatcg tgcacgttcg caccccacgg gaggtggccc cagcgcccgg tgtagcccgg
      721 aggccgctcc ggcttggaca gcacgtagaa ttcgtgcggg gcctgggtga ggtaatagag
      781 gtagctgccg tgggtgtgcc aggtgagcac tttcagccga cgcataccgc tcttcgatcg
      841 aacgggcgat agctgccgat gaggcggcgc gcctcgatgg tcacttcgtc gattccgacg
      901 cccgtcgcgc acggatggcc gatgggacac tggaggtacg cgcacggcct gcacccgacc
      961 ggatgatgga gcacgcagtg gcgatcgcgg tcgagcggcg cccagcgacg gaaatccgag
     1021 ccgcaacaga cgacgacact cggcgtgcct accgccgctg caatatgcga gacaccggtg
     1081 tcgttgcaga caatcaacgc tgccgcctcg acgagcgcgg caagcacccc aagcgttgtg
     1141 cctcccacaa ggttgaccgg gatgtgatgc atggtcgccg ccacatcctg cacgagaccc
     1201 gcttctccgg aagtgccggt caggacgatc ttgtagccgt cttccgccag ccgatcgccg
     1261 accgccgcga atcgcgcggc cggccaacgc cgcgacggaa gctgtgagcc cggatgcacg
     1321 cagacatagg agccgcgctc gagaccgtgc tcagcggcga gctgcccgca gtccgatcgc
     1381 tcctgcgccg tgatcggaaa ttccagtcga tcgtcgccca ccgggacgcc cagcgcccgc
     1441 atcagtctcg tgtaacgcag gacctcgtgc tcgtcctgac gccaagggag gaagcgctcg
     1501 cgatcgggac agtatgcgcc ctccgcgcag tagcccgcgt agtgccgcgc attcaccagc
     1561 gcggtgagcg gattgagcag acgaccgtct ccgtgcatct gcagggcgag atcaaagcgg
     1621 cgcgcctgca ccgcttcgag gaaccgcgga acgttcccga catcgcatac ccgctcgggc
     1681 aatcccggaa aacccgggaa ctcgatgaag tcatccacgt aacggcgaaa gcgcgcggca
     1741 aactcgcgcg cccacggcag gcccgcgagc gtcacgcgcg cggtcggaaa cgccgcgcgc
     1801 aacgcgcgca gcgccggcac ggcgcaaaga aggtcgccca gctgcagcgc acggatcacc
     1861 aggatcgtcc gcggcgcacc gggcagatcg cgcaccatcg cccggacttt ctctgcctct
     1921 tcaggcacgt tgcggtttct ccaggatgta ggacccgatg acgagcgcat cgagcggtga
     1981 ggtcatgaag cagcgtaccg cgtcctgcgg cgtgcacacg atgggctcac cttgggtgtt
     2041 gaacgacgtg ttgacgagga ccggcacgtc ggtgcggcgc tggaatgcct tgatgagccg
     2101 atggtagagc gcatgctgcg ccgcattgac ggtctggatg cgcgccgtac catccacgtg
     2161 gcacacgccg ggaatgcggt gcctgcgctc gcggacgacg tcgtgcacaa agagcatgaa
     2221 cggggacacc tgcgcgcccg caaaccattt gcccgcctct tcctcgagca ccaccggcgc
     2281 caccggtcga aaatcctcgc ggtccttgat ggcgttcaat cgttgctgta tttctgcggt
     2341 gagaggcgac gcgagtatcg agcgcgcgcc gagcgcgcgc gggccgaatt ccatgcggcc
     2401 ctgaaaccag cccacgatgc ggtccgcggc caggatgtcc gccgtttcct cggccacgtc
     2461 atgtgcgcgc cgatacggca gcttcgaata ttccagcagc gcacggatat c
//
LOCUS       DASURG010000199         5998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1159433, whole genome shotgun sequence.
ACCESSION   DASURG010000199 DASURG010000000
VERSION     DASURG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159433"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFY80_03345"
     CDS             <1..646
                     /locus_tag="VFY80_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6006969.1"
                     /translation="LIPVLILLFARTLGTVGAKPAAEVEQSVQAKNLLANAPLRRTLI
                     TGAAILTGIDLFQFYMPIYGNAIGLSATTIGLILSMFAAASFAVRLVMPALVKRWSAE
                     TVLLWSLYAGCVTYLVFPLFENAVLLAVIAFALGLGMGCGQPLTMSLIYSRAPAGRSG
                     EALGLRVSINNFMHIVVPLLFGTIGSMLGIAPVFVANALILGSGGVLIGRADRK"
     gene            674..1648
                     /locus_tag="VFY80_03350"
     CDS             674..1648
                     /locus_tag="VFY80_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX6006970.1"
                     /translation="MRIAVIDDYQDAFRQLACYKRLEDHQVDVYHDTEKDPARLAERI
                     KDAEALILTQQRSPFPRALAERVPRLKLISQTGRNANHIDVGACTEHGIVVCAGGAGN
                     PSPTAELAWGLILSSLRRIPQEVQRMKDGKWQGSVGVGVEGKTLGVYAYGRIGSIVAK
                     VGRAFGMRVVCWGREGSTSRAREAGFEVAPGRVAFFEQSDVISLHLPLNKETRGIIKP
                     EDLARMKPTALIVNTSRAGLIADGALVDALKRGRPGRAAVDVYDEEPVMGARHPLLAM
                     DNVVCTPHLGYVEERTYESYFGTAIEQILAFAAGKPVNVVNPEALGKR"
     gene            1651..3330
                     /locus_tag="VFY80_03355"
     CDS             1651..3330
                     /locus_tag="VFY80_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6006971.1"
                     /translation="MARLSCGRAAVEALRAAGVTKVFGLMGSSTLEMYDALYDAKDMA
                     YVGVRHENCGAHMADAYARVTQTPGLFVCGQAGPGSVNMMLGLAAAKMAYSPLVAITG
                     LPATEHLGRDAFQEIDQHTAFLPVTKRVLTVPRPERIPEFILEAFRIANSGRRGPVVV
                     MIPRDLFNHEIDVEPPDALPHMTGGAVDRHVVQRVCDMVMQAKRPLIHAGAGIKWSRG
                     TPSLVALAEKLQLPITTSAGHGDVAPNDHPLYAGTVGARGNPVATALMREADLILALG
                     TRLGFNTTRFQRDSFAPDVRIVQVDIDPIAVGRYFPVAVGIVGDAAAVAAALCETAAP
                     VPSAGAPWRARNEKFVAERRTLLQQREESGRRSGTPLHPDVIYAELREVAPRDALFTL
                     DAGTTGLQATDQLPYSATPALLTPLDCASIGFSYAAGLGAKVAAPRRTVISLMGDGGF
                     GMTMGEMATAVMHDIPTIAIVMDNGAWGSEIAYQRDYFNRRYIGAHVLSPRFDEVMKL
                     TGGVGYAVSERGETADAVRQAMKEGKPAVIHAKVDPDAVISFRTDALKKRT"
     gene            complement(3385..3933)
                     /locus_tag="VFY80_03360"
     CDS             complement(3385..3933)
                     /locus_tag="VFY80_03360"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HEX6006972.1"
                     /translation="MEVHVHGNAFLCKGVRLPQVEQALRPWLDYLDVDTIAEAASLER
                     EEPGIVFDARERTLNVCWTGEVGRSFHSRLTEAFHNLGPLTEYASEIEVTYYPEHGDD
                     EFYQMFVGPTSEAIHDFRRQCVVEDVSAMLARHLSKQEVDQVAALINQMFDSALTTKR
                     ASGEATVSSTVIPLHPRNKHLH"
     gene            complement(4026..4961)
                     /locus_tag="VFY80_03365"
     CDS             complement(4026..4961)
                     /locus_tag="VFY80_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6006973.1"
                     /translation="MANISGKAGWHPKREVEIVAGTPPGGGLDRTARALAAAIEAERL
                     LDVPVRVVNIAGDGARKAWRYVEQRASDAHVLAISSPNLTTDRLVGLARFDHSSFTPL
                     AILYNEYIAFIVPVDSPLRTAYDLVQVLATRSSRVTVALSTALGNPNHIALANVIRHA
                     GGDVTAPRIRVFDSALDALADALTGNADTAVITAASAVRALAAGTARALAVSAPQRLA
                     GMYASVPTWVENGVDCTVGAWRGVTAPSGLAPEEISYWERILRAAAESAAWTDALARQ
                     YWTGMYVDGDRLRHALAREAQEMATILTRLGLAKL"
     gene            complement(4969..5676)
                     /locus_tag="VFY80_03370"
     CDS             complement(4969..5676)
                     /locus_tag="VFY80_03370"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6006974.1"
                     /translation="MNGNWERKLGLIVPSWNTVMEYEVQRMAGPTMSAHSVRIPHTAD
                     TEENLLGLATHAPAVAKLLAHAKVHVIGYGCTAGGFLKGPEGDRKTGAEISESTRIPV
                     ATSATAVADALRAVGARRVGVASPYAAWLNERVRQFLEADGFEVIAIQGLGTEAHSTI
                     TLERVEALAAEVNRPEAQAIFISCSNFRTLEIIEPLERRFGKPVVTSNASTMWKMLRV
                     IGDKRAIAGAGRLFREA"
     gene            5783..>5998
                     /locus_tag="VFY80_03375"
     CDS             5783..>5998
                     /locus_tag="VFY80_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6006975.1"
                     /translation="MKACDFVRLCALLLLPAVAWLNASLACAQTYPSKAIRIVVPFPA
                     GGTSDILARALGQKLSEEWKQPVIVDNR"
BASE COUNT         1027 a         1921 c         2002 g         1048 t
ORIGIN      
        1 gctgattcct gtcctgatcc tcctgttcgc gcgcaccctc ggcacggtcg gcgccaagcc
       61 ggccgccgaa gtggagcagt cggtgcaggc gaagaatctg ctcgccaacg ctccactgcg
      121 tcgcacgctc atcacaggcg cagccattct gaccggcatc gatctcttcc agttctacat
      181 gccgatctac ggcaacgcga tcggcctttc agcgacgacc atcggactca tactcagcat
      241 gttcgctgcg gcgtcttttg ccgtgcggct ggtcatgccg gcgctggtca agcgctggag
      301 cgccgagacg gtgctcctgt ggtcgctcta tgccgggtgt gtgacctatc tcgtctttcc
      361 gctgttcgaa aacgcggtgc tgctcgcggt gattgcgttc gcgctgggcc tcggcatggg
      421 ctgcgggcag ccgcttacga tgtcgctcat ctacagtcgc gcgcccgcgg ggcgttcagg
      481 tgaggcgctc ggactgcgcg tcagcatcaa taacttcatg catatcgtcg tgccgcttct
      541 cttcggcacg atcggctcca tgctggggat tgcgccggta ttcgtcgcaa atgcgctgat
      601 cctgggaagc ggcggcgtgc tgatcgggcg cgcggataga aagtagaatg ccggttcacc
      661 cggaggaaaa ctcatgagaa tagcggtcat cgacgactac caggacgcat ttcgccagct
      721 cgcgtgctac aagcggctcg aggatcatca ggtcgacgtg taccacgata ccgagaagga
      781 tcctgcacgg ctggccgagc gcatcaagga cgccgaagcg ctgatcctca cccagcagcg
      841 ctcgccgttt ccgcgcgcgc tggcggagcg cgttccacgg ttgaagctca tcagccagac
      901 cggacgcaac gcgaaccaca tcgacgtcgg tgcctgcacg gagcacggca tcgtggtatg
      961 cgcgggcggc gccggcaatc cgagcccgac cgcggaactc gcgtgggggc tcatcctttc
     1021 ctcactgcgc cgtatcccgc aggaagtgca gcggatgaaa gacggaaaat ggcagggcag
     1081 tgtgggcgtc ggtgtggaag gcaagacgct cggggtctat gcctacggcc gcatcggcag
     1141 catcgtcgcc aaagtcggtc gtgcattcgg catgcgcgtc gtctgctggg gccgcgaagg
     1201 gtcgaccagc cgcgcgcggg aagcgggctt cgaggtcgcc ccgggccgtg tagcgttctt
     1261 cgagcagtcg gacgtgatct cgctccatct gccgctcaac aaggagacgc gcggcatcat
     1321 caagcccgaa gaccttgcgc gcatgaagcc gactgcgctc atcgtgaata caagccgtgc
     1381 cggcctgatc gcggacggcg ccctggtcga cgccctcaag cgcggccgcc cgggacgcgc
     1441 cgcggtggac gtatacgacg aagagccggt gatgggtgcg cgtcatccac tcctcgcgat
     1501 ggacaacgtc gtctgcacgc cgcatctggg gtacgtcgaa gagcggactt acgagtctta
     1561 cttcggcacc gcgatcgagc agatcctggc gttcgcagcc ggcaaaccgg tcaacgtggt
     1621 gaatcccgag gcgctcggaa agagataacg atggcaaggt tgagttgcgg ccgggcagcg
     1681 gtggaagcgc tgcgcgcggc tggtgtgacg aaggtcttcg ggctgatggg ctccagcacg
     1741 ctcgaaatgt acgacgcgct ctacgacgcg aaggacatgg cttacgtcgg cgtgcggcac
     1801 gagaactgcg gcgcgcacat ggcggatgcc tatgcgcggg tgacgcaaac gcccggcctc
     1861 ttcgtctgcg gacaggcggg tccggggtcg gtcaacatga tgctcgggct tgctgccgcg
     1921 aagatggcgt actcgccgct cgtggcgatc accgggctgc ccgcgacgga acatctcggt
     1981 cgcgacgcct tccaggagat cgatcagcac accgcgtttc tcccggtcac gaagcgcgtc
     2041 ctcacggtgc cgcgccccga gcgcattccg gaattcatac tggaagcgtt tcgcatcgcg
     2101 aattcgggaa ggcgcgggcc ggtggtggtg atgattccgc gcgatctctt caaccatgaa
     2161 atcgacgtcg agccgccgga tgcgctgccg catatgacgg gcggcgcggt tgaccggcat
     2221 gtcgtccaac gcgtatgcga catggtgatg caagcgaaaa ggccgctcat ccatgcaggc
     2281 gcgggcatca agtggagccg cggtacgccg agcctcgtgg cgctcgctga gaagctgcag
     2341 cttccgatca cgacttctgc gggacacggc gatgtggcac ccaacgatca cccgctttac
     2401 gcgggcacgg tcggtgcacg cggcaacccg gtcgcgaccg cactcatgcg cgaagcggat
     2461 ctcatcctcg cgctcggcac gcggctcggc ttcaatacga cgcgcttcca gcgcgacagc
     2521 ttcgcgcccg acgttcgcat cgtgcaggtg gacatcgacc cgattgcggt cgggcgttat
     2581 ttccccgtcg cggtgggcat cgttggagat gcggcggcag ttgccgcagc gctgtgcgaa
     2641 acggccgcac cggttccgtc cgccggcgcc ccgtggcgcg cccgcaacga aaaatttgtc
     2701 gccgagcgcc ggacacttct ccagcagcgg gaagaatcgg ggcgccgcag cggcacgccg
     2761 ctccatcccg atgtgatcta cgccgagctg cgggaggtcg cgccgcgtga tgcgctcttc
     2821 acgctcgatg cgggcaccac cggcttgcag gcgacggacc agctgcccta ctccgcgacg
     2881 cccgcgctgc tcactcctct cgactgcgcg agcatcggtt tttcctatgc cgcagggctt
     2941 ggggccaaag tcgcagcgcc ccgccgcacg gtgatctcac tgatgggcga cggtggattc
     3001 ggcatgacga tgggcgaaat ggctacggcc gtcatgcacg acattccgac catcgccatc
     3061 gtgatggaca acggggcctg gggttcggag atcgcctacc agcgcgacta cttcaaccgc
     3121 cgttacatcg gcgcgcacgt cttgagcccg cgcttcgatg aggtcatgaa actgacgggc
     3181 ggcgtcggct acgccgtcag tgaaagaggc gagactgcgg atgcggtgcg acaggcgatg
     3241 aaggagggca agcccgcggt catccacgcc aaagtcgatc cggacgcggt gatcagcttc
     3301 cgcaccgacg ccctcaagaa gcgtacctag gggagcgcgc ttccgcccgc aaagggcttg
     3361 ccggagaacg ggcgcagcgg cagcctagtg caggtgcttg ttccgcggat ggagcggaat
     3421 gaccgtcgag gagacagtgg cttcgccgct cgcgcgcttc gtagtaagcg cgctgtcgaa
     3481 catctggttg atgagcgcgg cgacctggtc gacctcctgc ttgctgaggt gccgcgcgag
     3541 catggccgac acgtcctcga ccacgcactg ccgccgaaaa tcgtggatcg cctcggacgt
     3601 cggtccgacg aacatctggt agaactcgtc gtcaccgtgc tcaggatagt aggtcacctc
     3661 gatttccgag gcgtactcgg tgagtggtcc caagttgtgg aatgcctcgg tgagccggct
     3721 gtgaaagctc cgcccgactt cgccggtcca gcagacattg agcgtgcgct cccgtgcgtc
     3781 gaagacgatg cccggctcct cgcgctccag gcttgccgct tcggcgatcg tatcgacgtc
     3841 gaggtagtcg agccacgggc gcagcgcctg ctcgacctgg ggcagccgca cgcccttgca
     3901 caggaatgcg ttaccgtgta cgtgcacttc cattcgcatg ggattccgct cgattcgggt
     3961 ggttcgccgc agcagcgcga cgaaaagtaa ggataacaca ccggcgccgc aagggctgcc
     4021 ctgcgctaca gcttggcgag tccgagccgg gtcaaaatcg ttgccatttc ttgagcctcg
     4081 cgtgcgagcg cgtggcgaag ccggtcgccg tcgacataca tgccggtcca gtactgccgg
     4141 gcgagcgcat ccgtccacgc ggcgctctcc gcagccgcac gcaaaatccg ctcccaatag
     4201 ctgatttctt ccggggccaa tccgctcggc gcggtgacgc cgcgccaggc gcccacggtg
     4261 cagtccacgc cgttctcgac ccacgtcgga acggacgcat acatgccagc gagccgctgc
     4321 ggcgccgaaa cggcgagcgc gcgcgccgtg cctgcagcga gcgcgcgcac ggcgctcgcc
     4381 gccgtgatga cggcggtgtc ggcgtttccc gtcagtgcgt ccgccagcgc gtcgagcgcg
     4441 gaatcgaaaa cgcgtatccg gggcgccgtc acatccccgc cggcatggcg tatgacattg
     4501 gcgagcgcga tgtggttggg attgccgagt gcggtggaaa gtgcgacggt cacacgggat
     4561 gaacgcgtag ccagcacctg cacgagatcg tacgcggtgc gcaaagggga atccacgggc
     4621 acgatgaacg cgatgtattc gttgtacagg atggcaagcg gggtaaagct cgaatgatcg
     4681 aagcgcgcga ggccgaccag ccgatcagta gtgaggttgg gcgagctgat ggcaagcaca
     4741 tgtgcgtcac tcgcacgctg ctcgacgtaa cgccacgcct tgcgcgcgcc gtcgccggcg
     4801 atgttgacca cgcgcaccgg cacgtcgagc aatcgctcgg cttcgattgc cgcggcgagc
     4861 gcgcgggcgg tgcgatcgag cccgccgccg ggtggcgtgc cggcgacgat ctcaacttcg
     4921 cgtttggggt gccagcctgc cttgccggag atgttcgcca ccgccgcgct aagcttcgcg
     4981 gaagaggcgc cccgcgccgg cgatcgcgcg cttgtcgccg atgacacgca gcatcttcca
     5041 catggtcgac gcgttgctcg tgaccactgg tttgccgaag cggcgctcca gcggctcgat
     5101 gatctcgagc gtgcggaaat tcgagcagct gatgaagata gcctgtgcct caggacggtt
     5161 gacttcggcg gcgagcgcct cgacgcgctc gagcgtgatg gtggaatggg cttcggtgcc
     5221 gagcccctgg atcgcgatca cttcgaaacc gtcggcttcg agaaactgcc gcacgcgctc
     5281 gttgagccaa gcggcgtagg gcgaggcgac gcccacccgg cgtgcgccca cggcgcgcag
     5341 cgcgtccgcg actgcggtgg ccgaagtcgc aaccgggatt cgggtcgact ccgagatttc
     5401 cgcaccggtc ttccgatcgc cttcggggcc tttcagaaag ccgcccgccg tgcagccgta
     5461 gccgatcacg tgtaccttgg cgtgggcgag cagctttgca acggcgggtg catgcgttgc
     5521 cagtccgagc agattttcct cggtgtctgc cgtatgggga atgcgcaccg aatgcgcgga
     5581 catcgtcggt ccggccatgc gttgcacctc atattccatg accgtgttcc acgagggcac
     5641 gatgaggccg agcttgcgct cccagtttcc gttcacgtcg ctgctcctgc gttcacgagc
     5701 gggaagaata ccagagccgc cacgcttgac gcatgcgggc gtgagcgaca tagtgcgccg
     5761 gatcggtaca tgggagaaga acatgaaggc gtgtgatttc gtgcgactgt gtgcgctcct
     5821 gctgctcccg gctgtcgcgt ggctgaatgc ctctttggcc tgcgcgcaaa cctacccttc
     5881 gaaagcgata cgcatcgtgg tgccgtttcc tgcaggcggc acgtcggaca tcctcgcgcg
     5941 cgctctgggg cagaagctct cggaggagtg gaagcagccg gtcatcgtcg acaaccgt
//
LOCUS       DASURG010000200         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_750692, whole genome shotgun sequence.
ACCESSION   DASURG010000200 DASURG010000000
VERSION     DASURG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750692"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..583
                     /locus_tag="VFY80_03380"
     CDS             206..583
                     /locus_tag="VFY80_03380"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEX6006976.1"
                     /translation="MRKALAFVGCLLGSAPVPSAEPAIHIVVQHSALAGFRHYEGHSL
                     WEAMRPGDDVALVREADNVYDPNAVRVEWRGHMLGYVPRRQNAHLARQLDRGVPLAGR
                     ITRLEKSRNGRNRVSYEILVPLE"
     gene            612..1400
                     /locus_tag="VFY80_03385"
     CDS             612..1400
                     /locus_tag="VFY80_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6006977.1"
                     /translation="MKLGRFALDGRAFYGVVEGDEVFELDGSPFKTYRRTERRHRIHA
                     LKLMVPCVPSNFYCAGLNYAAHIEWANRRTGGNRTPPEKADIGYRSPNALIPSGEAIV
                     IPADSPGPVQFEGELVAVIGKEARNLSETHALSCVLGYTLGNDVSERAWQKEDRTLWR
                     AKNCDTFKPMGPFIVTDLDPMNLDIAIHVNGAKVSEYHTGKMLFSAQHYIAQITRYMT
                     LWPGDVLWLGTDNATEPSLKHGDRVEVIQKDIGVLSNPVVRAAG"
     gene            1423..2559
                     /locus_tag="VFY80_03390"
     CDS             1423..2559
                     /locus_tag="VFY80_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX6006978.1"
                     /translation="MEAFFAPCPRGLERVLADELTALDAQDVHTVDGGASFSGAMRLA
                     YAVNLQSRVASRVLWRVGVAPYRSEHDVYAAARELPWNRWFDVRRTIRVNVTAIRAPV
                     KSRDFVTLRIKDAVCDVFRAAIGRRPDVDTVAPDVRVHAFLTKDEVAFYLDTSGDALF
                     KRGWRTAGGEAPLRENLAAGILRLTGWLPGTPLLDPMCGSGTFLIEAAAMALDIAPGL
                     ARAFGFERLHNFEADAWRAMRDRAALRRHPDRRLDIHGADKYGAVLETARENLRAAGM
                     ADAVELKQVDVLESSPPAARGIMVMNPPYGERLGSMESLASFYPLLGAVLKKRYANWI
                     AFILTADLRLPGLMRLKPARRTPLYNGALECRLFEFRLVSGSMR"
     gene            2671..3093
                     /locus_tag="VFY80_03395"
     CDS             2671..3093
                     /locus_tag="VFY80_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006979.1"
                     /translation="MEKEQTLRILNALANGVHPATGERFAEDSPYQHPDTVRALFEAI
                     RVIEQARPASGERGQRAAAIPGNTGLRWTSEEEERLITAFDAGRTVDELARAHNRTRA
                     GIEARLVKLGRLKESALTVQLRYPPKTGTGEPQTGRAE"
     gene            complement(3113..3700)
                     /locus_tag="VFY80_03400"
     CDS             complement(3113..3700)
                     /locus_tag="VFY80_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX6006980.1"
                     /translation="MLLDVSTALALGVRFSSAADRSELLLLAPCSLAGAVSGVTLLVT
                     LPRSATLAGFGAFLLGYGAYALAQGEAAGRVARAWGALAGFTGGAMGTLFGVGAPPYA
                     IYLARRLSDKAVLRATLSNIVLLSTTIRALVFLASGLMLADRIVGYALLLPFALGGLA
                     LGNRFHGRISRAALLKLISVLLALIGLSLLYRALA"
BASE COUNT          743 a         1261 c         1269 g          576 t
ORIGIN      
        1 cctgcccctc acgcacgcgg taagcgtcgc gcgtccgctc atgaacgacg caatgccgcc
       61 gaacgcagcg ctgcacctct cggtgctcgc gttctatgcc gtggtcgggt tttacatcgc
      121 cctcgtgctg acgcgccgcc ggctgctcaa gtagttctgg agcgcaaccg gtcatccgta
      181 cggcggcagc gccgttttag ccagcatgag aaaggcgctc gctttcgtcg gctgcctgct
      241 cgggagtgct cccgtcccat cggcggagcc ggcgatccat atcgtcgtac agcactcggc
      301 cctggccggt tttcgccact atgaggggca cagcctgtgg gaagccatgc gacccggaga
      361 cgacgtcgca ctcgttcggg aggcagacaa tgtgtacgac cccaacgccg tgcgggtgga
      421 atggcgcggg cacatgctgg gttacgttcc gcgccgccag aatgcccatc ttgcgcgcca
      481 gctcgaccgc ggcgtgccgc ttgccggccg gatcacgcgg ctcgagaagt cgcgcaacgg
      541 acgcaaccgt gtaagctacg agatcctggt gccgcttgag tagcggccgg tcgccacaaa
      601 ggaggtgaat catgaaactc gggcgtttcg ccctcgacgg cagagcgttt tacggcgtgg
      661 tggaaggcga tgaggtattc gagctggacg gctcgccgtt caagacgtat cggcggacgg
      721 agcggcggca ccggattcac gcgctgaagc tcatggtacc ctgcgtgccg tccaactttt
      781 actgcgcggg actgaactac gccgcgcaca tcgaatgggc gaaccgccgc acaggcggca
      841 accgcacgcc gccggaaaag gccgacatcg gctatcgctc gcccaacgcc ctcatcccct
      901 ccggggaggc aatcgtcatt ccagccgact cgccgggacc ggtgcagttc gagggcgaac
      961 tcgtcgccgt gatcggcaag gaagcgcgca atctttccga aacgcacgca ctctcctgcg
     1021 tactcggata caccctgggg aacgacgtga gcgagcgcgc ctggcagaag gaagaccgca
     1081 ccctgtggcg tgcaaagaac tgcgacacgt tcaagccgat gggaccgttc atcgtgaccg
     1141 atctcgatcc gatgaatctt gacatcgcga tccatgtaaa cggcgccaag gtttccgaat
     1201 atcacaccgg caagatgctg ttctcggcgc aacactacat cgcgcagatc acccgctaca
     1261 tgacgctgtg gccgggcgat gtgctgtggc tcggcaccga caacgcgacc gagccctcgc
     1321 tcaagcacgg cgatcgcgtg gaggtgatcc agaaggacat cggagtgctc tcgaacccgg
     1381 tggtgcgcgc ggcgggttga gcggcccaac aaccccttct tcatggaggc cttctttgcg
     1441 ccgtgcccgc gcgggctgga acgcgtcctc gccgatgagt tgaccgcgct cgacgcgcag
     1501 gatgtccaca ccgtcgacgg tggcgcctca ttttccggcg cgatgcgcct cgcctacgcc
     1561 gtcaacctcc agagccgcgt ggcaagccgc gtgctgtggc gcgtaggggt ggcgccctac
     1621 cgctcggagc acgatgtcta cgccgcggca cgggagcttc cttggaaccg atggttcgac
     1681 gtgcggcgta cgatacgcgt caacgtgacg gcgatccgtg ccccggtcaa gagccgcgac
     1741 ttcgtcacgc tgcgcatcaa ggatgcggta tgcgacgtgt tccgtgccgc aatcgggcgc
     1801 cgcccagacg tcgataccgt tgcgcccgac gtgcgcgtcc acgccttcct tacaaaggac
     1861 gaagtcgcgt tctatctgga cacatcaggc gacgccctgt tcaaacgcgg atggcggacg
     1921 gcaggcggtg aagcgccgct ccgcgagaat ctggcagcgg gcatcctgcg cctgaccggg
     1981 tggttgcctg gcacgccgct cctcgatccg atgtgcggca gcggcacgtt cctgatcgag
     2041 gcggcggcga tggccctcga catcgcgccg ggcctcgccc gcgcgttcgg attcgagcgg
     2101 ctgcacaatt tcgaagccga tgcctggcgt gccatgcgcg atcgtgcagc tctccgtcgc
     2161 catcccgatc gccgcttgga catccatggc gcggacaagt acggggcggt cctcgagacc
     2221 gcgcgcgaga acctgcgtgc cgcgggaatg gcagacgcgg tcgaactgaa gcaggtcgat
     2281 gtactggaat cgagcccgcc cgccgcacgt ggaatcatgg tgatgaatcc cccttatggg
     2341 gagcgcctcg gaagtatgga atcgctcgca tcgttctatc ccctcctcgg cgcggtgctg
     2401 aagaagcgct acgcgaactg gatcgcgttc atcctgaccg cggacctgcg tctcccgggt
     2461 ctcatgcgac tcaagccggc gcgccgcacg ccgctctata acggagcgct ggagtgccgg
     2521 ctgttcgagt tcagactcgt gagcgggagc atgcgctgag ccgatgcgag ttcacgtcaa
     2581 ccgcctggcg agcggacgcg ttggggaccg tacccgcatc cgacagggac gggcggaagg
     2641 cgacggataa cccgacgagg aggagacaaa atggaaaaag agcagacact ccggatactg
     2701 aatgcgctcg caaacggcgt gcacccggcg acgggcgagc ggtttgccga agacagcccc
     2761 tatcaacatc ccgacacggt gcgagcgttg ttcgaggcga tacgcgtcat cgaacaagcg
     2821 cgccccgcgt cgggggagcg gggacagcgg gccgcggcga tcccggggaa taccggattg
     2881 cgctggacct ccgaggagga ggagcggctg atcaccgcct tcgacgcggg gcgcacggtc
     2941 gacgaactcg cccgtgccca caatcgcacg cgcgccggca tcgaagcgcg tctcgtgaag
     3001 ctcggcagac tgaaagagtc agctctcacc gttcaactgc gctacccgcc gaaaactggg
     3061 acaggcgagc cccagaccgg gcgcgcagaa tgagagtggg cggcttgcgc gctcaagcaa
     3121 gcgcgcggta gaggagcgag agcccgatca gcgcgagcaa gacgctgatg agcttgagca
     3181 gcgccgcacg cgagatccga ccgtggaagc ggttgccgag cgcgagcccg cccagcgcaa
     3241 agggcaggag cagcgcgtag ccgacaatcc ggtcggcaag catgagcccg cttgcgagaa
     3301 atacgagcgc gcggatcgtc gtcgagagca gcacgatgtt ggacagcgtc gcgcgcagca
     3361 cggccttgtc cgagagtctc cgtgcgagat agatcgcata aggcggcgca ccgacgccga
     3421 agagcgtacc catcgcgccg ccggtgaacc cggcgagcgc gccccacgct cgagcgacac
     3481 gccccgccgc ctcgccttga gcaagcgcat acgcgccata gccgagcaga aacgcgccga
     3541 agcctgcaag cgttgcgctg cgcggcagcg tgacgagcag cgtcacaccg ctcaccgcgc
     3601 ctgcgagcga gcacggcgcg agcaggagaa gctccgaccg gtcggccgcg ctcgaaaatc
     3661 gcacgccgag cgccagggca gtcgagacgt ccagcaacac gcacggggca gcacgaattc
     3721 cagcgggaaa agatgcgtga gcagcggtac cgtaaagagc gcggcgccaa aggccgaaat
     3781 cccgaagaca acgtacgccc cgaacaccac tgccgcggca aaagcatacg ttggaaaatc
     3841 gaaaggaat
//
LOCUS       DASURG010000201         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_852712, whole genome shotgun sequence.
ACCESSION   DASURG010000201 DASURG010000000
VERSION     DASURG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852712"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFY80_03405"
     CDS             complement(<1..528)
                     /locus_tag="VFY80_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006981.1"
                     /translation="MQTLSAVDARLPRLTGPWAFALCVGLIAAIALLDYMTGHELSFS
                     ILYLAPVFLSAWRLGRDAGIVVSMMAAVTWLISVVFMGLEYSHPFYYFWDAALTFLTF
                     VLFALVISRLKTALQHADERFVTVLEGLDSAVFVTDASGRLLYGNEQYHKSAAAGTAL
                     LDAARVPVQTGSGAAG"
     gene            complement(547..1284)
                     /locus_tag="VFY80_03410"
     CDS             complement(547..1284)
                     /locus_tag="VFY80_03410"
                     /inference="COORDINATES: protein motif:HMM:NF023472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HEX6006982.1"
                     /translation="MKTLLMAATALFLSGLARAEADYVRERRWADEIVPAIVVGEPVY
                     LEAAGHKFLALHVPRRNAAAGLVIVHGMGVHPDWGLIGPLRTGLADAGYATLSIQMPV
                     LSADAEAERYNALFPEAAKRIESAIAFLRTGGVRKVAIVSHSIGARMANYFLANSTAP
                     RVDAWVAIGIPGDYVQTETLRLPVLDIYGEGDFPALLSNAEKRADAIRALRGSAQVEV
                     AGADHFFNGHESALVKYIQRFLDRALR"
     gene            complement(1319..2098)
                     /locus_tag="VFY80_03415"
     CDS             complement(1319..2098)
                     /locus_tag="VFY80_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6006983.1"
                     /translation="MSDPVLIARDGTIATVTLNNPERLNALNKAMWSGLGAAMHELSA
                     EEGLRCIVLRGAGEKAFAAGADISEFATERANSAQAREYGGLIHDTMQAVAHCRHPTV
                     AMIHGACVGGGLEIAAMCDMRICGESSRFGIPINRLGLTMAYGELQGLLALVGRAVAL
                     EILLEGRVFDAREALHKRLVNRVVPDAQVEEEAYALARRIADGAPLVARWHKKFIDRL
                     TVVAQPTPAEWDEGFACFDTEDYRTGVEAFLKKEKPRFTGR"
BASE COUNT          461 a          752 c          740 g          363 t
ORIGIN      
        1 gcccgcggcg ccggagccgg tttgcaccgg cacgcgggcg gcatcgagca gcgcggtgcc
       61 ggccgctgcg gacttgtggt actgctcgtt gccgtagagc agcctgccgc tcgcatccgt
      121 cacgaaaacc gccgagtcga gcccttcgag taccgtcacg aaacgctcat cggcgtgctg
      181 aagcgcggtc ttgagccggg aaatgacgag ggcgaacagc acgaaggtga ggaacgtgag
      241 cgcggcatcc cagaagtaat agaacggatg cgagtactcg agccccatga agacaaccga
      301 gatgagccat gtgacggcgg ccatcatcga aaccacaatg ccggcgtcgc ggccaagccg
      361 ccaggcggag aggaacacag gggcgaggta gagaatggag aacgacagct cgtggccggt
      421 catgtagtcg agcagcgcaa tggccgcgat cagcccgacg cacagcgcga atgcccatgg
      481 accggtcaga cgcggaaggc gcgcgtcgac cgcggataag gtttgcaagg aggcctcacc
      541 caccgcctac ctcagcgcgc ggtcgagaaa gcgctggata tacttcacga gcgcgctttc
      601 gtggccgttg aagaaatgat ccgctcccgc cacttcgacc tgcgccgaac cgcgcagcgc
      661 acggatggcg tcagcgcgtt tctcggcgtt cgagagcagt gcggggaaat caccctcccc
      721 ataaatatcg aggaccggca gccgaagggt ttcggtctgg acgtagtcgc ccgggatgcc
      781 gatcgcaacc caggcatcga cacgcggagc ggtgctgttc gccagaaagt aattcgccat
      841 cctcgccccg atgctgtgag agacgatcgc gaccttccgg acgccgccgg tacgcagaaa
      901 cgcgatggcc gattcgattc gcttggccgc ttcggggaag agcgcgttgt accgttcggc
      961 ttccgcatct gcgctgagca ccggcatttg tatggaaagt gtcgcgtatc ccgcgtctgc
     1021 gagacccgtg cgcagcgggc cgatgagtcc ccagtccgga tgtacgccca tcccgtggac
     1081 gataaccaat cccgccgcag cattgcgccg cggaacgtgc agcgcgagaa acttgtgacc
     1141 cgcggcctcg agatagaccg gctcgccgac cacgatggcc ggcacgatct catcggccca
     1201 gcgccgttca cgcacataat cggcctcggc acgcgcgagt cccgagagaa acagcgccgt
     1261 ggcagccatc aagagagttt tcatggagat gaaacgccat cggacgatgg cgaagcgcct
     1321 atcggcccgt gaagcggggt ttctcctttt tcaagaacgc ctccacgccg gtgcgatagt
     1381 cctcggtatc gaaacatgca aaaccttcgt cccactctgc cggagtgggc tgtgcgacga
     1441 ccgtcagccg atcgatgaat ttcttgtgcc agcgggcgac gagcggcgcg ccatccgcga
     1501 tccgccgggc gagcgcatac gcctcctcct cgacctgcgc gtcaggcacg acccgattca
     1561 cgaggcgctt gtgcaacgct tcgcgagcat cgaagacccg gccttcgagc agaatttcga
     1621 gcgcgacggc gcgcccgaca agtgccagca ggccctgaag ctcaccgtag gccatcgtca
     1681 gcccgagccg gttgatgggg atgccgaagc ggctcgattc gccgcagatg cgcatgtcgc
     1741 acatcgcggc gatctcgagc ccgcctccga cgcacgcgcc gtggatcatc gcgacggtcg
     1801 gatgccggca atgggcgacg gcctgcatag tgtcgtggat caacccgccg tattcgcgtg
     1861 cttgcgcgga atttgcacgc tcggtggcga attccgagat atccgcgcca gctgcgaacg
     1921 ccttctcgcc cgcgccccgt agcacgatgc aacgcaagcc ctcctcggcg gaaagctcgt
     1981 gcatcgccgc cccaagcccg gaccacatcg ccttgttgag ggcgttgagg cgctcgggat
     2041 tgttcagggt gaccgtggcg atcgtgccgt cacgggcaat cagtacggga tcggacatag
     2101 cgtgcgggca tccagagtgt atgccaaaac gcctgccccg gaaacgccgc ggcggtccgc
     2161 ggcgcgcgat gtcaccagtt gatccagctc actggaccca agctgatcca cggcacgtag
     2221 gtcacgagca gcgtgacgag caccagtacc atcgtgaacg gccagcccac cctgaagact
     2281 tcccagaacg aaatgcgggc gatcgctgcc gtggtg
//
LOCUS       DASURG010000202         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_852930, whole genome shotgun sequence.
ACCESSION   DASURG010000202 DASURG010000000
VERSION     DASURG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852930"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFY80_03420"
     CDS             complement(<1..339)
                     /locus_tag="VFY80_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6006984.1"
                     /translation="MQQILVVDDEVGIRELLSEILSDEGYQVRLAENAGEARAFRTRT
                     RPDLVLLDIWMPDTDGITLLKEWATAGLLTMPVVMMSGHGTIDTAVEATRIGAYDFLE
                     KPIALQKLLAT"
     gene            complement(353..2563)
                     /locus_tag="VFY80_03425"
     CDS             complement(353..2563)
                     /locus_tag="VFY80_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6006985.1"
                     /translation="MNDAVKAPVRPGGRAKYLLMLCVGLGGVAVFLLATASASTRFFA
                     EHYTLLLFLNGGVALTLAALVVYQLVTLRRKLRAGVFGAKLTLRLVLLFALMAVLPGA
                     LIYAVSVQFLAKGIESWFESNLDRALESGLNLGRGVLDNMLRDLTAKGDAMALALSAR
                     PPSEHVGTLNALREQAGAPEATLFTNRGKVIAFSASERVGLLPEPPSADVLRQIRMQR
                     GYGGIESIPDRGLYLRVLVPVNVVSLSEETRVLQLLRPVPPELARDAETVQAGYREYQ
                     EHTLVRRGLKRLYGITLTLTLLLALLSALALAFLLSDRLSAPLGVLVEGTRAVAQGDF
                     SPRAAVASRDELGTLTQSFNSMTGQLAEARAMAERNQAELQHAKARLESILANLSAGV
                     LTFDDHLRLHSANPSAAGILGVDWKPLQEVPVHEWALRDESLASLGQAIAEAFERAGA
                     KEWERQIERKTAGETQILLLRGTQLPRVAETSSVVVFDDITHLLQAQRAAAWAEVARR
                     LAHEIKNPLTPIQLSAERLQAKLTAKLAPPDADMLARSTQTIVNQVASLKRMVDAFSQ
                     YARTPEPSMRELDLNGVVREVLTLYESLGSRIRLELAPALPPIIGDAAQLRQVIHNLL
                     QNAQDALGEMESPRIVVATEAVDGRVRLAVTDNGTGFPEHLMSRVFEPYVTTKPGGTG
                     LGLVIVKKIIEEHGGEVTITNVAPHGARVTATLPVAAEARRAVAESRRAGQGVN"
     gene            complement(2560..3138)
                     /locus_tag="VFY80_03430"
     CDS             complement(2560..3138)
                     /locus_tag="VFY80_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX6006986.1"
                     /translation="MTASTMRFCRSASWLAAFAALCFVLGLAFPARADIEVKKASLAV
                     AEDGYVLEADFSITLTPPLEDVLSKGVSLYFLLDFELIRPRWYWFNEKITESQHQYRL
                     SYNALTRQYRVGTGTLYQNFPTLSEALDVMSHIRRQRELEPGTLKRDTTYIAAVRLRL
                     DTSQLPKPFNLTALGSRDWNIASDWYRWTVNP"
     gene            complement(3101..4384)
                     /gene="rsmB"
                     /locus_tag="VFY80_03435"
     CDS             complement(3101..4384)
                     /gene="rsmB"
                     /locus_tag="VFY80_03435"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX6006987.1"
                     /translation="MEEAQRLAAAIVLQVLNGTSLDAALRAMREKHPGLSPPHRALVQ
                     ELSFGTLRYLGHVDALLEALLTKPLRDERLRSLLRVALYQLEYTRAAAHTVVDQAVLA
                     TARLGHTAAKGLVNAVLRNFLRRRGELNTELRRSETARLSYPRWWIIKLRQQYPQRYG
                     DILEAGNLHPPMTLRANRRRLSASEYLARLHAEGIAAHELEGEAITLEKPRPVVDLPG
                     FAEGLVSVQDASAQRAAHLLDLRAGQTVLDACAAPGGKSAHILELADVRLTALDVDLA
                     RLERLRANMQRLGLAARLAHGDATDPSAWWDGERFDRILADVPCSASGVVKRHPDIKW
                     LRRPSDIPQFAALQARVLEALWQLLAAGGKLLYATCSVFSEENRLQIDAFLERHSDVH
                     RLTLPGDDINREQPAGQILPDERHDGFYYALLQKR"
     gene            complement(4387..>4640)
                     /locus_tag="VFY80_03440"
     CDS             complement(4387..>4640)
                     /locus_tag="VFY80_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6006988.1"
                     /translation="ALDPAPGAYAACGDGVLKIWRARVERGRSSPPGTILAVDGSGIL
                     VSCGEGALRITELQRPGGRRVPAAAYLAGHTLAPGTRFG"
BASE COUNT          828 a         1517 c         1501 g          794 t
ORIGIN      
        1 cgtcgcgagc agcttttgca gcgcgatcgg tttttccagg aagtcgtagg caccgatacg
       61 ggtcgcttcc accgccgtat cgatggtgcc gtgaccggac atcatgacga ctggcatggt
      121 cagcagcccc gcggtggccc actccttcag aagcgtgatg ccgtcggtat ccggcatcca
      181 gatgtcgagt agcacgaggt cggggcgcgt tcgggtgcgg aaggcgcgcg cttccccagc
      241 gttctcggcc aaccgcacct ggtacccctc gtcggaaagg atttccgaaa gcagttcgcg
      301 gataccgact tcgtcgtcta ctacgaggat ctgttgcata gggtcctgaa gtttagttta
      361 caccttggcc cgcacggcgc gattcggcga cggcgcgccg tgcctctgcc gcgacaggca
      421 gtgtcgccgt cacccgcgcg ccatgcggcg cgacgttcgt aatggtcacc tccccgccgt
      481 gctcctcgat gatcttcttc acgatcacga gaccgagccc cgtgccgccc ggttttgtcg
      541 taacgtaagg ctcgaagacg cggctcatga gatgctcggg gaaccccgtg ccattgtcgg
      601 tgacggccag gcgcacgcgt ccgtcgaccg cttcggtagc cacgacgatc cgcggcgatt
      661 ccatctcgcc cagagcatcc tgcgcattct gcagcaggtt gtggattacc tgccgcagct
      721 gtgcggcgtc gccgatgatc ggcggcagtg ccggcgcaag ctccagtcgg atgcgcgagc
      781 cgagggattc gtacagtgtg agcacctcgc gcaccacgcc gttcagatcg agctcgcgca
      841 tcgagggctc cggtgtgcgc gcgtattggc tgaaggcatc gaccatgcgc ttcaacgagg
      901 cgacctggtt gacgatggtc tgcgtcgagc gcgcgagcat atcggcgtcc ggcggcgcga
      961 gcttggcggt caacttcgcc tgcaggcgct ccgccgaaag ctggatcggc gtcagcggat
     1021 tcttgatctc gtgcgcgagg cggcgcgcga cttccgccca cgccgcggcg cgctgtgcct
     1081 gaagcaagtg cgtgatgtcg tcgaatacga ccacggagct cgtctccgcg actcgcggca
     1141 gctgcgtgcc gcgcagaagg aggatctgcg tttcacctgc cgtcttgcgc tcgatctgcc
     1201 gctcccattc ctttgcgccg gcgcgctcga atgcttcggc gatggcctgg ccgagcgaag
     1261 cgagagattc atcgcgtagc gcccactcgt gcaccggcac ctcctgcaac ggcttccaat
     1321 caacgcccag tatcccggcc gcgctcggat tcgcggaatg caggcgcaaa tgatcatcga
     1381 atgtgagcac gcccgcggaa agattcgcga gaatgctctc caggcgagcc ttggcgtgct
     1441 gcagctccgc ctggttgcgc tcggccatcg cgcgcgcttc ggcgagctgg ccggtcatgc
     1501 tattgaacga ctgtgtgagc gtgccgagct cgtcgcggct cgcaactgcg gcgcgcgggc
     1561 tgaaatcgcc ttgtgcgaca gcgcgcgtgc cttctaccag cacgcccaac ggcgccgaaa
     1621 gccggtcgga cagaaggaaa gcaagcgcca gtgcggagag cagcgcgaga aggagggtga
     1681 gcgtcagtgt gatgccatag aggcgcttca aaccacggcg caccagcgta tgctcctgat
     1741 actcgcggta tcccgcctgc acggtctcgg cgtcgcgcgc gagctcgggc ggcaccgggc
     1801 gtagcagctg cagcacccgc gtctcttcgc tgagcgaaac gacgttgacc ggcacgagca
     1861 cgcgtagata caggccgcgg tccggtatcg attcgatacc gccgtagccg cgctgcatgc
     1921 ggatctggcg cagcacgtcc gcgctcggcg gctcgggcaa aagccccacg cgctcgctcg
     1981 cggagaaggc gatcaccttg ccgcggttgg taaaaagcgt cgcttccggg gcaccggcct
     2041 gctcccgcaa agcgttgagc gtgccgacgt gctcggatgg cggtcgtgct gacagcgcaa
     2101 gcgccatcgc gtctcccttg gcggtaaggt cgcgcaacat gttgtcgagc acaccccgtc
     2161 cgagattgag gccgctctcc agggcccggt cgagattcga ctcgaaccat gactcgatcc
     2221 ccttcgctag aaattgcacc gaaaccgcgt agatcaacgc ccccggcagg acggccatca
     2281 gcgcaaacag cagcacgagt cgcaatgtga gcttggcgcc gaagacgcct gcccggagtt
     2341 tcctgcgcaa cgtaacgagc tggtagacga cgagcgcggc gagcgtgagt gccacgccgc
     2401 cgttcaggaa gagcagcagc gtgtagtgct cggcgaaaaa acgcgtgctt gcgctcgctg
     2461 tggcaagcag aaataccgcc acgccgccca gccccacaca gagcatgagc agatatttgg
     2521 cacgacctcc cggccttacc ggagctttca cggcgtcatt catgggttca ccgtccagcg
     2581 ataccagtcg gaggcgatat tccagtcccg cgagccgagc gcagtgaggt tgaacggctt
     2641 cggcagctga gaggtgtcga gccgaagccg cacggccgca atgtaggtcg tatcccgctt
     2701 caacgtaccg ggctcgagtt ccctttggcg gcgaatatgg ctcatcacgt cgagcgcctc
     2761 ggacagcgtc ggaaaattct gatagagggt gcccgtgccg acccgatatt ggcgcgtcag
     2821 cgcgttatac gaaagccgat actgatgttg cgactcggta atcttctcgt tgaaccagta
     2881 ccagcgcggc cggatcagct cgaagtcgag gagaaaataa agtgacacac ccttgctcaa
     2941 tacgtcttcg agcggcggcg tgagcgtgat agaaaagtcc gcctcgagca cgtagccgtc
     3001 ctccgcgact gcgagactcg ccttctttac ttcgatatcg gcgcgggcgg ggaaggcaag
     3061 accgaggaca aagcaaagcg cggcaaacgc tgcaagccag ctagcgcttc tgcaaaagcg
     3121 catagtagaa gccgtcatgc cgttcgtccg gaagaatctg gcccgcgggc tgttctcgat
     3181 tgatgtcatc ccccgggagg gtcaggcggt ggacgtcgct gtggcgctcc aggaaggcgt
     3241 cgatctgtaa ccggttctcc tcggagaaca ccgagcacgt cgcatacagc aatttaccac
     3301 cggcggccag caactgccaa agcgcctcca gcacgcgcgc ctgcaatgcc gcgaattgcg
     3361 gaatatcgct cggtctgcgc agccacttga tatccgggtg gcgtttcacg acgcccgaag
     3421 ccgagcaggg cacgtcggcc agtatccggt cgaaacgctc cccgtcccac cacgcagaag
     3481 gatccgtcgc atcgccatgc gcgagccttg ccgcaagacc gagtcgctgc atgttggcgc
     3541 gcaggcgctc caggcgcgcg aggtccacgt cgagcgcggt gagccgcacg tctgccagct
     3601 cgagaatatg cgcgctcttt ccgcctggag ccgcgcacgc atcgagtacg gtctggccgg
     3661 ccctcaaatc gaggaggtgc gcggcgcgtt gcgcggaggc gtcctgcacc gataccagcc
     3721 cttccgcgaa tccggggagg tcgacgaccg gacgcggttt ctcgagcgtg atcgcctcgc
     3781 cctcgagctc atgtgccgct atgccctcag catgaaggcg ggcgaggtac tcgctcgcac
     3841 tcagacgacg gcgattggcg cgcaaggtca tgggcggatg caggtttccc gcttccagga
     3901 tgtcgccata gcgttgcgga tattgctggc gcagcttgat gatccaccac cgcggatagg
     3961 acaaccgcgc ggtctcgctg cgccgcagtt ctgtattgag ctcgccccgc cgacgcagga
     4021 aattgcgcag gaccgcgttg acgaggcctt tggcggcggt gtgtccgagg cgtgcggtcg
     4081 cgagcaccgc ttggtcgacg acggtgtgcg cggccgcgcg ggtgtactcg agttggtaga
     4141 gcgccacgcg gagaaggctg cgaagccgct cgtcgcgcaa cggcttggtg agcaatgcct
     4201 cgagcaacgc atccacatgc cccagatacc gaagcgttcc aaagctcagc tcctgcacga
     4261 gcgcccggtg cggcggcgaa agcccggggt gcttctcgcg catggcgcgc agagccgcgt
     4321 ccaggctcgt gccgttcagc acctggagca cgattgcggc agccagccgc tgcgcttctt
     4381 ccatggctaa ccgaatcggg tgccgggagc aagcgtatgg ccggccagat acgcggcggc
     4441 cgggacgcgg cgcccgccag gacgctgcaa ctccgtgatg cgcagcgcac cttccccgca
     4501 cgagaccaga attccgctgc catcgaccgc cagtatcgtg ccgggcggcg acgacctccc
     4561 gcgttcgacg cgcgcgcgcc aaatcttgag cacgccgtcg ccacacgccg cgtatgcgcc
     4621 cggcgccggg tcaagcgcgc
//
LOCUS       DASURG010000203         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_241243, whole genome shotgun sequence.
ACCESSION   DASURG010000203 DASURG010000000
VERSION     DASURG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241243"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1860
                     /locus_tag="VFY80_03445"
     CDS             151..1860
                     /locus_tag="VFY80_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6006989.1"
                     /translation="MAKKFEDVSQPRDYELIVEKDVKITLRDGAILYADVFRPDGGSE
                     RFPAIMNMGPYQKDRLWIPPDDLEEEANPHLAWETGNPMWWCPRGYALVRVDSRGSGK
                     SPGQSDPNSYQEGVDFHDAIEFVAKLPWCSGNIGTLGVSYHANSQWRAANTQPPSLKA
                     IIPWEGRADLYRDQAFHGGIFAMGFLQNWIAANIAHNLIGRARSYNPNAFNNNMYWQF
                     AANSLDSEFWRMRSARWDHITVPTYAVGNWTGMGLHLRGATEAFMHAATRHKKMRIHS
                     GTHFHPFHSEEGRLDQLRFFDYWLKGKDTGIMDEPPVKLMIRTGGDMTTKGYKFRFEN
                     EWPLARTRWTKLYLKLNTDGQSKNGEPEGELIASPPAPSMAATYQASPPSHAGVTSSS
                     PAQTFGMIGRAGISFDTPPLPEDTEVTGPIKLVVWVSSTSEDMDIYATIRNIGLDGKD
                     VWEAGQQGWDEVPVAKGWLRVSHRELDPERSLPYRPYHAHKQRQWLNPGEVVECHVEI
                     WPTSMVFKKGHRIRLDIQPRDGAGSSVYRHYSADYNIGARNSVHAGGDRSSYLMLPVI
                     PTV"
     gene            complement(1925..>2395)
                     /locus_tag="VFY80_03450"
     CDS             complement(1925..>2395)
                     /locus_tag="VFY80_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6006990.1"
                     /translation="DAPGGGLTATGIHILDAFVAVAGPVDAVHAHVMSHPPAPEPVDS
                     STVFLEFSSRVSGVLCGVRTTPAYWRVHVFGKEASAEALGATELTVRRTDAEPERFTF
                     EPVDTLRLELEAFADAASGGAPYYIAPQQMIDTVAALEATIESMKSGGTVRVRR"
BASE COUNT          460 a          788 c          743 g          404 t
ORIGIN      
        1 cgcagcattc gctgccgcct tcgcttgcag ccagcggcgg tgcgattcga ccgcgcgcga
       61 gcttggcgat atgctcagcc aatatcatcg agtggctgcg tgagcccttc gatgcgccgc
      121 cccatatctt ccacaacagt cgagagcgga atggccaaga aattcgaaga cgtgtcgcaa
      181 cctcgcgatt acgagctgat cgtcgagaag gacgtgaaga taacgctgcg cgacggcgcc
      241 atcctctacg ccgacgtgtt tcggcccgac ggcggctccg agcgcttccc ggcgatcatg
      301 aacatgggcc cttatcagaa ggaccgcctg tggataccgc cggacgatct ggaggaagag
      361 gcaaatcccc atctcgcctg ggagaccggc aatcccatgt ggtggtgccc gcgcggctat
      421 gccctcgttc gcgtcgactc gcgcggctcg ggaaagtcgc cgggtcagtc ggaccccaat
      481 tcataccagg agggcgtgga ttttcatgac gcgatcgaat tcgtggcgaa gctgccgtgg
      541 tgctccggca acatcggcac gttgggcgtg tcctaccacg cgaacagcca gtggcgcgcg
      601 gccaatacgc aaccgccgtc gctgaaagcc atcattccgt gggaaggacg cgccgacctc
      661 tatcgggacc aggcgtttca cggcggcatc ttcgccatgg gttttctgca gaactggatc
      721 gccgcgaaca tcgcgcacaa cctcatcggc cgtgcccgca gctacaaccc caatgccttc
      781 aataacaata tgtactggca gttcgcggcc aacagtctcg attccgaatt ctggcggatg
      841 cgcagcgccc gatgggacca cattacggtc cccacgtatg ccgtaggcaa ctggaccggc
      901 atgggcctgc atcttcgcgg cgccaccgaa gcgttcatgc acgccgccac caggcacaag
      961 aagatgcgca tacacagcgg cacccatttt catccgttcc attcggagga aggccgcctc
     1021 gatcagctgc gcttcttcga ttattggctc aagggcaagg acacggggat catggacgag
     1081 ccgccggtga agctgatgat ccgcaccggg ggcgacatga cgacaaaggg ctacaagttc
     1141 cgtttcgaga acgagtggcc tctggcgcgg acccgttgga cgaagctcta cctcaagctc
     1201 aacaccgatg gtcagagcaa gaacggggag cccgaaggcg aactgatcgc gtcgccgccg
     1261 gcgccatcga tggcggccac ttatcaggcg agcccgccct cgcacgcagg ggtgacctcg
     1321 tcctcgccgg cgcaaacgtt cggcatgatc gggcgcgccg ggatctcgtt cgacacgccg
     1381 ccgctgccgg aagataccga ggtgaccggt ccgatcaagc tggtggtgtg ggtgtccagc
     1441 acttccgagg acatggatat ctacgcgacg atccgcaaca tcggcctcga cggcaaggac
     1501 gtctgggagg cgggccagca aggctgggac gaagtgcccg tagcgaaggg ctggttacgc
     1561 gtctcgcaca gggagctcga tcccgagcgc tcgttgccgt atcggccgta ccatgcgcac
     1621 aagcaacggc agtggctgaa tcccggcgag gtggtcgagt gccacgtcga gatctggccg
     1681 accagcatgg tgttcaagaa gggacaccgc atccgcctcg acattcaacc gcgcgacggc
     1741 gccggctcgt ccgtgtaccg gcactattcg gccgactaca acatcggtgc ccggaattcg
     1801 gtgcatgccg gcggcgacag gtcctcttac ctgatgctcc ccgtcattcc gacggtctga
     1861 cggtcgtcga tcgccagtcg agcgggggct acacgaagct cggtcggtgc gcccgctcga
     1921 caactcaacg ccggacccga accgttccgc ccgacttcat cgactcgatc gtcgcctcca
     1981 gcgccgccac ggtatcgatc atctgctgcg gcgctatgta gtagggcgca cctccgctgg
     2041 cggcatcggc gaacgcctcc agctcgaggc gcagcgtgtc caccggctcg aatgtgaacc
     2101 gctcgggctc ggcgtcggtt cttcgcacgg taagctccgt cgccccgagc gcttcggcag
     2161 aagcctcctt gccgaagacg tgcacacgcc agtacgcggg cgtcgtgcgc acgccacata
     2221 gcacgccgct cacccggctg gagaactcga gaaaaaccgt cgagctgtcg accggctccg
     2281 gcgccggcgg atgcgacatc acatgcgcgt gcaccgcatc gactggaccg gcaacggcga
     2341 cgaacgcatc gagaatgtgt attccagttg cggtgagccc tccgccgggc gcgtc
//
LOCUS       DASURG010000204         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1057332, whole genome shotgun sequence.
ACCESSION   DASURG010000204 DASURG010000000
VERSION     DASURG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057332"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1190)
                     /gene="tal"
                     /locus_tag="VFY80_03455"
     CDS             complement(78..1190)
                     /gene="tal"
                     /locus_tag="VFY80_03455"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX6006991.1"
                     /translation="MNRSTNPLRALHALGQRVWLDNLSRTLLREGTLERLVREDGLAG
                     VTSNPTIFYKAISGSAYYRDELAALRREGMLAPEARYERLAIPDIQAACDLLRPLHRA
                     TEGEDGYVSLEVSPALANDTAQTVAAARRLHALVARENVLIKVPATPAGIAAIEELIG
                     VGISINVTLMFSLAHVHAVARAYLRGLERWIAGDGDARRIKSVASLFLSRVDTLVDQK
                     LDTVSAPEELKGKTGVALAKLAYQEYRKIFKAQGPFTALAARGGRPQFMLWASTGTKN
                     PAYSDLLYVEPLIGPETINTLPDATLNAFRDHGRAALTLESGIEDASRHFERLARLGI
                     DLNEVGETLQVEGVKLFMQSFDELLGLLAAPASSAA"
     gene            1315..2559
                     /locus_tag="VFY80_03460"
     CDS             1315..2559
                     /locus_tag="VFY80_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX6006992.1"
                     /translation="MLQGAFGFVALLALAWLAGENRRAVPWRIVIPGVLLQLVLTAVL
                     LTAPPVKQLFLVLNEMLLALERATRAGTSFVFGYLGGATLPFPESGMGSSFVLAFRAL
                     PLVLVVSALSALLFYWRVLPFVVRSISALLEKSLGVGGAVGLSTAANIFIGMVEAPLF
                     IRPYLAQMSRGELFIVMTCGMAGIAGTVMVIYAAMLSAVVPDAMGHILAASVVSAPAA
                     IVVSALMVPPVGEPTAGRVAAPAVAKSAMDAVTRGTVEGLALLLNIVALLIVLVALVT
                     LANIALGILPEVGGAPVTLQRMLSVVMAPVVWLIGIPWSESQAAGALMGTKTILNEFI
                     AYSDLAQLPPDALSPRSRLIMTYALCGFANFGSLGILVGGLATMVPERRDEVVALGMR
                     SLFSGTLATLVTAAGVGMLLGD"
BASE COUNT          432 a          850 c          940 g          530 t
ORIGIN      
        1 gcggctggag gcaagcgcgt cggccgcgtt gtagcgaggc tgcggcgccc gcccgcgcat
       61 gcaaccggac cgaaagctca ggccgcgctg ctcgccggcg cggccagcag acccaggagc
      121 tcgtcgaagg actgcatgaa aagcttgacg ccttcgacct gcaacgtttc cccgacttcg
      181 ttcaggtcga tgccgagccg ggcgaggcgt tcgaaatgcc ggctggcgtc ctcgatgccg
      241 ctttccagcg tcagcgcggc ccggccgtga tcgcggaacg cgttgagcgt tgcatcgggc
      301 agggtgttga tcgtctccgg gccgatgagc ggctcgacgt agagcaggtc gctgtaggca
      361 gggttcttcg tgcccgtact cgcccacagc atgaattgcg gccggccacc gcgtgcagcg
      421 agtgccgtga acggtccctg cgccttgaag atcttccgat attcctggta ggcaagcttg
      481 gcgagcgcaa cgccggtctt gcctttgagt tcttcaggcg cgctgacggt atcgagcttc
      541 tggtcgacga gcgtgtcaac acggctcaga aagaggcttg ctaccgactt gatgcgtcgt
      601 gcatcgccgt cacccgctat ccaccgctcg agcccgcgca gataagcccg cgcgacagca
      661 tggacgtgcg cgagcgagaa catcagcgtc acgttgatcg aaatgccgac gccgatcagt
      721 tcctcgatgg cagcgattcc ggcgggtgtc gcgggaacct tgatgagcac gttttcgcgg
      781 gcaacgagcg cgtgcaggcg tcgcgccgcg gcgacagtct gtgccgtatc gttcgcgagc
      841 gcgggagaca cttcgagact cacatagcca tcttcgcctt ccgtcgcgcg gtgtagcggg
      901 cgcaacaggt cgcatgccgc ctggatgtcg ggaatggcga gccgctcgta gcgcgcctcg
      961 ggcgcgagca taccctcgcg ccggagcgcg gcgagttcat ccctgtaata ggcgctcccg
     1021 ctgatggcct tgtaaaaaat ggtcgggttg gaagtgacgc ctgcaaggcc gtcctcgcgc
     1081 accagccgct cgagtgtgcc ctcgcgcaac agcgtgcggg agagattgtc gagccagaca
     1141 cgctgaccga gcgcatggag cgcgcgcagt ggattcgtcg atcgattcat acgggccgag
     1201 cgtagcgaag cgcgttcacc gggtcaatac gcatctcccg catgcgcttg ttgagcggtg
     1261 gaaggaaggt taacgtaccg agaacgggga aaggggagaa cgccgttgct gtcagtgctg
     1321 cagggcgcat tcggattcgt ggcgctgctc gcgttggcat ggcttgcggg cgagaaccgg
     1381 cgcgccgtgc catggcgtat cgtgattccg ggcgttctgc tccagctcgt tcttacggca
     1441 gtactgttga ccgccccacc ggtaaagcag ctttttctcg tgttgaacga gatgctgctc
     1501 gcgctggagc gcgcgacgcg cgcgggtacg tcattcgtat tcggctatct gggtggagcg
     1561 acgctgccct ttcccgagtc gggcatggga tcgagcttcg tgctcgcttt ccgcgccctg
     1621 ccgctcgtcc tggtggtgag cgcgctctca gcgcttctct tttactggcg cgtgctgccc
     1681 tttgtggtcc gatcgatctc cgcgctgctc gaaaagtcgc tcggtgtcgg cggcgcagtg
     1741 ggactgtcca cggcggccaa catcttcatc ggcatggtgg aagcgccgct cttcatccgg
     1801 ccttatctcg cgcagatgag ccgcggagaa ctctttatcg tcatgacgtg cggcatggcg
     1861 ggcatcgcgg gaacggtcat ggtcatttac gccgccatgc tgagtgcggt ggtgcccgac
     1921 gcgatgggac acatcctggc tgcgtcggtt gtcagcgcgc cggccgccat cgtggtgagc
     1981 gcgctcatgg tgccgccggt cggcgaaccg acggcggggc gagtggctgc gccggcggtg
     2041 gcgaagagcg ccatggatgc cgtcacgcgt ggaacggtgg aagggcttgc gctgctgctc
     2101 aacatcgtgg cgctgctcat cgtcctggtg gctcttgtca cactcgccaa tattgcgctc
     2161 ggcatcctgc cggaggtggg aggtgcgccc gttacgctgc agcggatgtt gagcgtggtg
     2221 atggcgcccg tcgtgtggct catcgggata ccgtggagcg aaagccaagc cgcgggcgcg
     2281 ctgatgggga ccaagacgat actgaacgag ttcatcgcct acagcgacct ggcgcagctc
     2341 ccgcccgatg cgctgtcccc gcgcagccgc ctcatcatga cttacgcgtt gtgcgggttc
     2401 gcaaacttcg gaagcctcgg tattctcgtc ggagggctcg ccaccatggt gccggagcgg
     2461 cgggacgagg tcgttgcgct cggcatgcgc tcgctctttt ccggcacgct cgcgacgctg
     2521 gtgaccgcag cgggcgtcgg catgttgctc ggcgactgaa gccctctcga cccggggtgg
     2581 cataatgccg gcgaggaggc cgtatgaccc ggattgtcgc agtgaagacc catcctgttt
     2641 ccataccgct cgggcacgcg ctctggacgg cgcacgaaaa actgacgact tcgagcctga
     2701 tcctgaccga ggtgcgcacc gacgacggcc tcgtgggcta cggtttgatc ca
//
LOCUS       DASURG010000205         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_956594, whole genome shotgun sequence.
ACCESSION   DASURG010000205 DASURG010000000
VERSION     DASURG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956594"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="VFY80_03465"
     CDS             complement(<1..1980)
                     /locus_tag="VFY80_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6006993.1"
                     /translation="MDMIPHEHIAFRMTGHSGPGSDSVVPPGLRPALFAHYRDLTALR
                     YDFPLVLVKGGQEADSIRSLSGVIDAVLQEVAARGADGERLRQHVLRLEKEIRALTAR
                     GVVGTLAALWEKSARRLAAASDDAVQESLEQARAAIKLDGKLVDCNAALAPRVLTHLW
                     EEVQQARTQPTLAQINALIVRLSDILRAGTLATEAGRSAEALKAGVGSVHEATFDFNM
                     MSRLLARVAGASTLRPSRLRRIEWALSVLRSQPFFHTGKEDPKRTAAKYNFVFDSCAA
                     ACETYGERLPRMVELLKAIAVARLEAEGRYIEGRHDAFFAAFDGGAVDRDDLSLFPDY
                     LVCMRWSAGDPADEAGLWHLLSSGIPAKVLVQCDEILVPANDHETVPFLSARSAHLAA
                     MAMGLRHTYVLQTVSANLLQAKDALLKGLRYPGPALFSVYSGCAASSTGIPPYLNAAA
                     AMESRAFPTFCYDPSKADWASAFSLEHNPDVTADWTTHNLGYEDAAHQRKTEEVKFTF
                     ADFVACNPRYARHYALVPREKWNESMAPVAECHAGGMYRGDARDPYVLLVDGSERLHR
                     AIVDDRLVSETERCRAAWRRLQELAGIHNSHAERCLARERQVWEEQKARELASLAKEP
                     RAVEPSAPTGEARAAAAKPVPAAAPSTEPAAAEVSG"
BASE COUNT          340 a          680 c          644 g          353 t
ORIGIN      
        1 gcccgacacc tctgcggcgg ccggctcggt actcggcgct gccgcgggaa cgggcttcgc
       61 cgcggccgct cgcgcctcgc ccgtcggggc ggagggctcc actgcacgcg gctccttcgc
      121 gaggctcgcg agttcgcgcg ccttctgctc ttcccatacc tgcctttcgc gggcgaggca
      181 ccgttcggcg tgcgaattat gtatgccggc aagctcctgc agcctgcgcc atgccgcccg
      241 gcagcgttcg gtttcgctca cgagccggtc atccacgatc gcccggtgaa ggcgctccga
      301 accgtccacg agcagcacat acggatcgcg cgcgtccccc cgatacatgc caccggcgtg
      361 acactctgca acgggcgcca tgctctcgtt ccatttttcg cgcgggacca gggcatagtg
      421 gcgggcatag cgcggattgc aggcgacgaa gtccgcgaat gtgaacttca cctcttccgt
      481 cttgcgctgg tgcgccgcat cttcataacc aaggttgtgc gtcgtccagt cggcggtcac
      541 gtcgggattg tgctcgagcg aaaatgcgga ggcccagtcg gctttcgacg ggtcatagca
      601 gaatgtcgga aacgcgcgtg actccatcgc cgccgccgca ttgaggtaag gcggaatgcc
      661 ggtcgaagag gcggcgcatc ccgagtaaac gctgaacagc gcgggccccg gatagcgcag
      721 gcctttcagt agggcgtcct tggcctgcag caggttggcg ctgaccgtct gcagcacata
      781 cgtgtggcgc aagcccatcg ccatggccgc gaggtgagcg ctgcgggcac tgaggaatgg
      841 aacggtttcg tgatcgtttg ccggaaccag aatctcgtcg cattgcacga gaacctttgc
      901 cggaatgccc gaagagagca gatgccacaa gcctgcctca tccgccggat cgccggcact
      961 ccagcgcatg cagacgagat aatcggggaa gaggctcagg tcgtcgcgat ccacggcgcc
     1021 gccgtcgaat gccgcgaaga aggcgtcgtg cctgccctcg atgtaacggc cctctgcttc
     1081 gaggcgcgcc acggcgatcg ccttgagtag ttcgaccatg cgcggcagcc gttcgccgta
     1141 ggtctcgcat gcggcagcac aactgtcgaa gacgaagttg tatttcgcag cggttcgctt
     1201 cggatcctcc ttaccggtat ggaagaatgg ctgcgaccga agaaccgaca acgcccactc
     1261 gatgcgccgc aagcggctag ggcgcagcgt actcgctccc gccacgcggg cgaggaggcg
     1321 cgacatcatg ttgaagtcga acgtcgcctc gtgcacgctg cccacgccag ccttgagcgc
     1381 ttcggcgctg cggccagctt cggtcgccag cgtgccggct ctcaggatgt cggacagcct
     1441 gacgatcagc gcattgatct gtgcgagtgt cggctgcgtc ctcgcctgct gaacctcttc
     1501 ccagagatgg gtaagaaccc tcggtgcaag cgcggcgttg cagtccacga gcttgccgtc
     1561 caacttgatt gctgcgcgcg cctgctcgag gctctcctgc accgcgtcgt ccgaggccgc
     1621 agcgagccgg cgcgccgatt tctcccataa tgccgcgagc gtgcccacca caccgcgcgc
     1681 cgtgagcgcc cggatctcct tctccagtcg gagtacgtgt tggcgcagtc gctcgccgtc
     1741 tgcaccgcgg gctgcgacct cctgcagcac ggcgtcgatg acgcccgaga gcgagcgtat
     1801 cgaatccgcc tcctgcccac ccttcacgag cacgagcgga aagtcgtagc gcagcgccgt
     1861 gagatcgcgg taatgcgcaa agagcgcggg gcgcaatccc ggcgggacca cgctgtcgct
     1921 ccccgggccc gaatgacccg tcatgcggaa ggcaatgtgc tcgtgaggaa tcatgtccat
     1981 gtcgcgatcg cctcgtaacc tcaaggaccc ggcttca
//
LOCUS       DASURG010000206         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1467929, whole genome shotgun sequence.
ACCESSION   DASURG010000206 DASURG010000000
VERSION     DASURG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467929"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFY80_03470"
     CDS             <1..569
                     /locus_tag="VFY80_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX6006994.1"
                     /translation="IADRFCTGSSIMPQKKNPDVAELIRGKTGRVNGHLVALLTLMKS
                     QPLAYNKDNQEDKEPLFDTVDTLMMSLAVFRDMLGGITVNKEAMRKAAQEGYATATDF
                     ADYLVRKGVPFREAHEAVARAVRLAAERGCDLAEITLQELRQFSPLIDEDVFEALKLE
                     GSLKSRSHLGGTAPVRVKAAIAKARKSL"
     gene            579..1448
                     /locus_tag="VFY80_03475"
     CDS             579..1448
                     /locus_tag="VFY80_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX6006995.1"
                     /translation="MWHAIAATIAAATGNPFTVERRTAVTGGCINRAFRIEGGGAAYF
                     VKVNAANAGEMFAAEAAALEEISRGATVRVPRVVCHGASPTQSWLVTEYIALSPTGDF
                     RMLGRRLAAMHRVTSRSFGWWRDNTIGATPQVNSPAARWPDFWREQRLGFQLRRAAER
                     GYGGGLQRKGEKLLARLDLILAHHAPPASLLHGDLWSGNMAFDAEGMPVIFDPATYYG
                     DREADLALTELFGRLPQAFYSAYREAYPVEAGYALRKDLYNLYHVLNHLNLFGAGYLR
                     QAESLMVRLLDRA"
     gene            complement(1453..>2491)
                     /locus_tag="VFY80_03480"
     CDS             complement(1453..>2491)
                     /locus_tag="VFY80_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6006996.1"
                     /translation="LPSGVKGEKITRAATLVDVARLARVSTATVSRALTLPHKVRART
                     LMRVQHAVQSLGYVAHGAARALASRRTRTIGAVIPTLDNAIFANTTHALQRTLDESGY
                     TLLLASHEFDAEVEVRATRALIERGIDGLVLLGTSHHPAVFRMIGTNALPYVLTWALD
                     ASGMHPCIGFDNRSAAIKLTEYLLEIGHREFAMISGLTANNERARERIEGVREALARR
                     GARLPDERVVEKAFTLGAGREGLREVMRGTPPPTVVMCGNDILALGAMAECNALALTV
                     PRDVSITGFDDMEIAALITPGLTTVHFPTAELGAYAARHLLQKLSGDTVEQRRELPVS
                     LVVRGTTAAPS"
BASE COUNT          442 a          806 c          826 g          417 t
ORIGIN      
        1 acatcgctga ccgcttctgc accgggtctt cgatcatgcc gcagaagaag aatccggacg
       61 tcgccgagct gatccgcggc aagaccgggc gcgtcaacgg ccacctagtc gcgctgctca
      121 ccctcatgaa gtcgcagccg ctcgcctaca acaaggacaa ccaggaggac aaggagccgc
      181 tgttcgacac ggtggatacg ctgatgatga gccttgccgt gtttcgcgac atgctgggcg
      241 gtatcaccgt caacaaggag gcgatgcgca aggcggcgca ggaaggttat gcgacggcca
      301 ccgatttcgc ggattatctc gtgcgcaagg gtgttccctt ccgcgaggcg cacgaggctg
      361 tcgcgcgcgc ggtacgcctt gccgccgagc gcggctgtga tctcgctgaa atcacgctcc
      421 aggagctgcg gcagttctcg ccgctcatcg acgaggacgt gttcgaagcc ctcaagctcg
      481 aaggctccct caagtcgagg agccacttgg gcggcactgc gccggtgcgg gtgaaagcgg
      541 ccattgccaa ggcacgcaag agcctgtaag gccccccgat gtggcacgcg atcgcagcga
      601 cgattgcggc tgcaaccggc aacccgttca cggtcgaacg ccgcacagcg gtaaccggcg
      661 gctgcatcaa tcgggctttc cgcatcgagg ggggcggcgc agcctacttc gtcaaggtca
      721 acgccgcgaa tgccggcgag atgttcgccg cggaagcggc agcgctcgag gaaatctcac
      781 ggggcgcaac ggtgcgtgtg ccgcgcgtag tgtgccacgg cgcgagcccc acgcagagct
      841 ggctcgtaac ggaatacatc gcgctctccc ccacgggcga cttccggatg ctgggacgcc
      901 ggcttgcggc aatgcaccgc gtcacgtccc ggagtttcgg ctggtggcgc gacaacacga
      961 tcggcgcgac accgcaggtc aactcgccgg ccgcgcgctg gcccgacttc tggcgcgagc
     1021 aacggctcgg ctttcaattg cggcgcgcgg ccgagcgcgg ctacggcggc ggactgcagc
     1081 gaaaaggcga gaaactgctc gcaagactgg atctcatcct tgcacatcac gcgccgcccg
     1141 cctcactgct gcacggggat ctctggtccg gaaacatggc tttcgacgcg gaaggcatgc
     1201 cggtgatctt cgacccggcc acctattatg gcgatcgcga agccgatctc gccctgaccg
     1261 agcttttcgg tcgcctgcca caggcgttct actccgccta ccgcgaggcg tatccggtcg
     1321 aggcgggcta cgctctacgc aaggaccttt acaatctgta ccacgtgctc aatcacctga
     1381 atctcttcgg agcgggctat ctgcggcagg cggaatccct catggtgcgt ctactggatc
     1441 gcgcgtgacc ggtcagctcg gcgccgccgt cgtgccgcgc acgacgagcg agacaggaag
     1501 ttcgcgacgc tgctcgacgg tatcgcccga gagcttctgc aggaggtgcc gcgccgcgta
     1561 ggcgccaagc tcggcggtcg gaaaatgaac cgtcgtcaga ccgggtgtaa tgagggctgc
     1621 gatttccatg tcgtcgaagc cggtgatcga aacgtcgcgc ggcaccgtga gcgcgagcgc
     1681 attgcactct gccatcgcac cgagcgcaag tatgtcgttg ccgcacatca ccacggtggg
     1741 cggcggcgtg ccgcgcatga cttcgcgcaa gccttcgcga cccgcgccca gcgtgaaagc
     1801 cttttcgacg actctctcgt cgggaaggcg ggcgcctcgt cgcgccagtg cttcgcgcac
     1861 gccttcgatg cgctcgcgcg ctcgctcgtt gtttgcggtg agcccggaaa tcatggcgaa
     1921 ttcgcggtga ccgatctcca ggagatactc cgtgagcttg atggcggcgg agcggttgtc
     1981 gaaaccgatg cacggatgca tgccgctcgc atcgagcgcc catgtgagaa cgtagggcag
     2041 cgcattcgtc ccgatcatgc ggaaaaccgc gggatggtgc gaggtcccga gcaacacaag
     2101 accgtcgatg ccccgctcga tcagcgcccg cgtcgcccgc acctcgactt ccgcgtcgaa
     2161 ctcgtgtgag gcgagcagca gggtgtagcc cgattcatcg agcgttcgct ggagggcgtg
     2221 cgtcgtgttg gcgaaaatgg cgttgtctag cgtaggaatg accgcgccaa tcgtgcgtgt
     2281 gcggcgggaa gccagggcac gggcggcgcc gtgcgcgacg tagcccagcg actgaacggc
     2341 atgctgcacc cgcatcaagg tgcgcgcgcg aaccttgtgc ggcagcgtga gcgcgcgcga
     2401 taccgtagcc gtggagacgc gcgcgagccg ggcgacgtcg actagtgtag ccgccctcgt
     2461 gattttttcg cctttcacgc ccgaaggcag c
//
LOCUS       DASURG010000207         7079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_650494, whole genome shotgun sequence.
ACCESSION   DASURG010000207 DASURG010000000
VERSION     DASURG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650494"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="epsG"
                     /locus_tag="VFY80_03485"
     CDS             <1..738
                     /gene="epsG"
                     /locus_tag="VFY80_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HEX6006997.1"
                     /translation="RFGEAAIWLGLITEQDLHLALARQYDFPQLASGDAAVSPELVAA
                     YAPFHRRVEELRALRTQLLIRWFNSTHQRRVLAIVSPETGEGRTYICANLAIVFSQLG
                     ERTLLIDADLRAPRQHRLFNVPDRIGLSTVLAGRVDSSAAVPVPGFAGLSLLPAGAPP
                     PNPQELLSRAGLARLLQEMQSKFDVILIDTPPAKLYADAQSVTYHAGGAVVIARKDHT
                     RLADTKSVIRDLNDTGAQIVGSVMNAF"
     gene            797..2287
                     /locus_tag="VFY80_03490"
     CDS             797..2287
                     /locus_tag="VFY80_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6006998.1"
                     /translation="MAVLTPQVPMRAEARWETFRDDRTSRASLNSVTTRLAIFAGVAF
                     ICLFWGYAVVVSEWKAVIVGVALIASVLILVDFRIGVVLLVLLMPISASKIFPHQIGG
                     ITGLNPVNLLLVATLGSYILHALLDRSIGRFIPRPLLLLYAVPFLIAGFLGTRHVGEI
                     SLYFVLTEHVKFVDSLGYLRDLVIKPLLLVFFAVLVAAAVARMQRPEKLLGPFIVSVW
                     VMGLMVVVYFVLSGANLAQLASSREREFLSPLGLHANELGRMYAIVYALLLFTWSQTR
                     DYALKLTLLASIGVVVLALVLTFSRGGFVGFMLVSVLFLISRRHIGSLLVTAVVAAAA
                     LYFLPDAIYHRVTTGVGMGANAVSAGRIDNIWMPLIPDLLKSPFYGQGIGAMLWSDAV
                     RGGKSLLVTHPHSAYLQTVLDMGLIGLALIGAYFFHLWRGFRALGADLSLNASERGFF
                     RGAAAALLAFLIMGFADGSFTPAPEQAFLWLAVGMMYGERARRAAS"
     gene            2291..4219
                     /gene="asnB"
                     /locus_tag="VFY80_03495"
     CDS             2291..4219
                     /gene="asnB"
                     /locus_tag="VFY80_03495"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX6006999.1"
                     /translation="MCGITGYWARNADPRAWLRDLHRSIRSLRARGPDDQGAWVREGD
                     EVAFGHTRLSILDLSTAGRQPMVAADGDFVLAFNGEIYNFRTIKAELASLGHVFRGSG
                     DTEVVLAALRQWGLNAVQRFIGMFAIALWSERARRLWLIRDRLGVKPLYYAWDGRVLW
                     FGSELKALCAFAAWRPELDRDALGDYFQYGYISAPRTIYRNVHKLQPGHWLELRTHGE
                     PTTGCYWSALEPREPLAGSEEALEAELEALFIDAFRYRMVSDVPVGVFLSGGLDSSLV
                     SAILARHSGQAIHTFTVGFTDPRFDESPWARRVAAHVDAHHTERILAPDAMKQTLRRW
                     GELFDEPFGDSSGVPALLVSQVAREQVKVALSADGGDELFSGYEQYGVVLARARTMAR
                     LPRSVRQVLWQTLRALPERGAAGADKGLPLPRSMQHALRRSVFERVERMCDVLPELDA
                     ASLCAVAASSWRSQDLAHLLGCQPAPRSALNGHPRSLLDYMSCWDIRHYLPDDILTKV
                     DRTTMAVGLEGREPLLDHRIVEFALRLPITLRRGALGTKHILRRVLYRYVPRELVERP
                     KKGFSIPLSRWLRSELAGLIDQYLAPERVKGAGILDPDEVARAVRCFREGGPRRDRLD
                     LQKVWLLLAFEMWRDRWA"
     gene            4203..5450
                     /locus_tag="VFY80_03500"
     CDS             4203..5450
                     /locus_tag="VFY80_03500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6007000.1"
                     /translation="MTGGRDEETGNVRILYVISGLGPGGAEKQLIELARELGRRGHHI
                     AIYVLTRDVSRAVDLAGSGVMLVVDAKRAKLDWGVVARLRRTLDRWRPDVVHGFLFDG
                     NFYSRIAAVGTGIPVLNSERSNGYALSATQRLAHRLTRACASGVVANTYCGARFAQSL
                     FGLPDERIHVVWNGIRIEELERQRSTGVDYRLEFFHERNVRLACLVGSIRPIKDYLLA
                     LETAARLIERDPAWRVLLIGDSLAPGAAYAEGAPASSNGYKAEVVARYRQIGLEDKIR
                     FCGLRGDVPAILHQCDALFVTSVNEGFPNAVLEAMALGVPVVSTDYSDIRRILPLQEQ
                     VVPTRDADGIARAILWAHSQRELLAARQRDWVCNHATMERAARALEEVYSTYAGLRGA
                     RAQHEADGAPQAYAPAVQAERER"
     gene            5447..6925
                     /locus_tag="VFY80_03505"
     CDS             5447..6925
                     /locus_tag="VFY80_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX6007001.1"
                     /translation="MSAQHDQARAHTLRRLRQRALSLGGANAFDYITQFLLPVVLARF
                     LDTLGFGQYRLLWLATRTALSIAPLAMPASLYYFLPRSDGADRRLYVNQTLFFLAVTG
                     LLSGWAVSSWNPWLPQSMQALEQHGAMVPVFVALWILASLLDMLPAIEERVSWQTWAI
                     IGLAVLRTVALSVAAIVTGELGAVLLVMIAFVAFKLALLGIYIAKYHGLRGPIVRWKA
                     FAQQMKHATPFGAAGALYDLRAQADQWIAATLFSLHLFAAFSVGAVIGTLVSIFRKSV
                     NTAFLPGMSRLEAAGDLNGMLELNSRANAMVGALVCPLAAFAFVYAEEVVTLIYTEAY
                     ISAAPVMRVYIIGLLALIVELASITLLLRQGPFVVRVNIAALVIAIPVSWLAARHYGL
                     AGAAAGSVIAIYLDRLVTLKHISRLSGVPLRRLQDWNALATLLVYSMLSAALAWWVTA
                     SYFPEPAPFIRLVVGGSTLGAAYLLLLNIGNFMNGLAVAGKR"
BASE COUNT         1098 a         2284 c         2265 g         1432 t
ORIGIN      
        1 cgtttcggcg aagccgcgat ctggctgggt ctcatcacgg aacaggacct tcacctcgcg
       61 ctcgcgcggc agtacgactt tccgcagctc gcgagcggcg atgcggcggt cagtcccgag
      121 ctcgtcgcgg cctatgcacc gtttcatcgg cgcgtggaag aattgcgtgc attgcgtacg
      181 cagctgctca tccgctggtt caatagcacc catcagcgcc gggtgctcgc aatcgtcagc
      241 cccgagaccg gggaaggacg gacctacatc tgcgcgaatc tcgcgatcgt cttttcccag
      301 ctcggcgaac gcaccctttt gatcgacgcg gatctgcgcg cgccgcgcca gcaccgtctg
      361 ttcaatgtgc cggaccggat cgggctttcc accgttctcg ccgggcgcgt cgattcgagc
      421 gctgccgttc cggtgcccgg attcgccggc ctttcgctgc tgcccgccgg cgcaccgcca
      481 cccaatcccc aggagctcct gtcgcgggcc gggctcgctc gactcctgca ggagatgcag
      541 agcaagttcg atgtgatcct catcgatacg ccgccggcca agctttatgc cgatgcgcaa
      601 agcgtgacct atcacgccgg cggtgcggtc gtgatcgcac gcaaggacca tacgcgcctc
      661 gccgacacca agagcgtcat acgtgacctc aacgatacgg gagcgcagat tgtcggcagc
      721 gtcatgaatg ccttctgaag cgaaggtgcg gcttccgcat ccagcggtcg cctgctctca
      781 ctaacggagg cattggatgg ccgtacttac tccacaagtc cccatgcgcg ccgaagcacg
      841 ctgggagacc tttcgcgacg accgcacgtc ccgggcgtcg ctgaactcgg taacaacccg
      901 gctcgcgatc ttcgcgggcg tggccttcat ttgcctgttc tggggctacg ccgtcgtcgt
      961 gagcgaatgg aaagccgtga tcgtgggcgt cgcgctcatc gcgagcgtgc tgatcctggt
     1021 cgatttccgc atcggtgtgg tgctgctcgt gctgctcatg ccgatctcgg cgagcaagat
     1081 cttcccgcac cagatcggcg gcatcacggg gttgaatccg gtcaacctgc tgctcgtggc
     1141 gacgctcggc tcctacattc ttcacgcgct tctcgaccgc agcatcggac gcttcattcc
     1201 ccgcccgctg cttcttctgt atgccgtgcc ttttctcatt gccggattcc tcggcacgcg
     1261 ccatgtcggg gagatctcgc tctatttcgt cctcaccgag cacgtcaagt ttgtcgatag
     1321 cctcggttac cttcgtgacc tcgtcatcaa gcctctgcta cttgtgttct tcgcggtgct
     1381 ggttgcagcg gccgttgcac ggatgcagcg gccggaaaag ctgttgggcc cgttcatcgt
     1441 gtcggtgtgg gtcatggggc tcatggtggt ggtgtatttc gtactgtccg gcgccaattt
     1501 ggcgcagctt gcgagcagcc gcgaacggga attcctgtcg ccgctcggat tgcacgcaaa
     1561 cgagctcggt cgcatgtacg caatcgtgta cgcattgtta ctgttcacgt ggtcgcagac
     1621 tcgagactac gccctgaagc tcacactgct cgcctccatc ggcgtcgtgg tactcgcgct
     1681 ggtgctgacg ttttcccgcg gcggcttcgt cggattcatg ctggtgagcg tgctgttcct
     1741 gatctcgcgc cgccacatcg gaagcctgct cgtcaccgcg gttgtcgccg ccgcggccct
     1801 ttacttcctg ccggatgcga tctatcaccg cgtcacgaca ggggtcggca tgggcgcaaa
     1861 cgccgtgagt gccggacgga tcgacaatat ctggatgccc ctcattcccg atctgctgaa
     1921 aagcccattc tacggtcagg gcatcggcgc gatgctgtgg tcggatgcgg tacgcggagg
     1981 gaaaagcctg ctcgtcaccc atccgcacag cgcctatctg cagaccgtac tggatatggg
     2041 gctgatcggc cttgcgctga taggtgccta cttcttccat ctgtggcgtg gttttcgcgc
     2101 actgggggcg gacttgagct tgaacgcgag cgagcgcgga tttttccgag gcgctgcggc
     2161 ggcattgctc gcgttcctca tcatgggctt cgcggatggc agtttcacgc cggctccgga
     2221 gcaagccttt ctctggctgg cggtaggaat gatgtatggc gaacgagcca gaagagcggc
     2281 gagctaggcg atgtgcggaa tcaccgggta ctgggcgcgg aatgccgatc cccgagcgtg
     2341 gctgcgcgac ctccaccggt cgatccgcag cctcagggcg cgcgggcccg atgaccaggg
     2401 cgcatgggtg cgcgagggcg atgaggtcgc cttcggccac acgcgactct ccatactcga
     2461 cctctcgaca gccggccgcc aacccatggt cgcggcggac ggcgatttcg tgctcgcctt
     2521 caacggcgag atctacaact tccgcaccat aaaggcggaa ctcgcgagcc tcggccacgt
     2581 cttccgtggg agtggggaca ccgaagtcgt gctcgcggca ctccggcaat ggggactcaa
     2641 cgcggtacag cggttcatcg gaatgtttgc gattgcgctg tggtccgagc gcgcgcgccg
     2701 gttgtggctc atacgggatc ggctcggcgt gaaaccgctc tattacgctt gggacggccg
     2761 cgtgttgtgg ttcgggtccg agttgaaggc attgtgcgct ttcgccgcgt ggcgccccga
     2821 gctggaccgc gacgcccttg gcgactattt ccagtacggt tatatctccg caccccgcac
     2881 catttaccgg aacgttcaca agctgcagcc cgggcactgg ctcgagctgc gcacccacgg
     2941 tgagccgacc acgggctgct actggtcggc gctcgagccg cgcgagccgc tcgccggttc
     3001 cgaggaggcg ctggaagccg agctcgaagc gctcttcatc gacgcctttc gctatcgcat
     3061 ggtctcggat gttcccgtcg gcgtttttct ttccggcggg ctcgattctt cactggtgag
     3121 cgcgatactc gcacgccact cggggcaggc gatccacacc ttcaccgtag ggtttaccga
     3181 cccgaggttc gatgaatcgc cctgggcgcg ccgcgttgcg gcccatgtcg atgcgcatca
     3241 caccgagcgc attctcgcgc cggacgcgat gaagcagacg ctccggcgct ggggcgagct
     3301 tttcgacgaa ccgtttggcg attcatccgg tgttcccgcg cttctggttt cgcaggtggc
     3361 gcgcgagcag gtgaaagtcg cgctgtccgc ggacggcggc gatgagctgt tcagcggcta
     3421 tgagcaatat ggcgtcgtgc tggcgcgcgc gcgtacgatg gcacgcctcc cgcgctccgt
     3481 gcgccaagtc ctgtggcaga cgctgcgggc gctgcccgag aggggtgcgg ccggcgcgga
     3541 taaggggctg ccgcttccgc gctcgatgca gcatgcgctg cggcgctcgg tcttcgagcg
     3601 ggtcgagcgc atgtgcgacg tcctgccgga gctggatgcg gcttcattgt gcgccgtggc
     3661 cgcatcttca tggcggtccc aggatctcgc ccaccttctc ggatgccagc ccgcgccccg
     3721 gtccgctctc aacggccatc cgcgttcgct gctagattac atgagctgct gggatatccg
     3781 ccattacctg ccggacgaca tcctcaccaa agtggaccgc acgacgatgg ccgtcggtct
     3841 cgaaggtcgc gagccgctcc tcgatcatcg catagtcgaa tttgcgcttc gtctgccgat
     3901 tacgcttcga cgcggggcgc tcgggacgaa gcacatcctg cggcgcgttc tctatcgata
     3961 tgtcccgcgg gaactcgtgg agcggccgaa gaagggcttc agcatccccc tttcacgctg
     4021 gctgcggagc gagcttgcgg gactgatcga ccagtatctc gcccccgagc gcgtgaaggg
     4081 agccggcatt ctcgatcctg atgaagtggc gcgcgcggtg cgctgcttcc gtgaaggcgg
     4141 gccaaggcgc gaccgcctgg acctgcagaa agtgtggctg ctgctcgctt tcgagatgtg
     4201 gcgtgaccgg tgggcgtgac gaggagaccg gaaacgtgcg catactgtac gtgatcagcg
     4261 ggctgggacc gggcggcgcg gaaaagcagc tcatcgagct tgcgcgcgag ctcggccggc
     4321 gcggccatca tattgcaatc tacgttctga cgcgcgatgt atcccgtgca gtcgatcttg
     4381 ccggttccgg cgtaatgctg gtggtcgatg cgaagcgcgc gaagctcgac tggggcgtcg
     4441 tggcgcgctt gcgtcgcacg ctcgatcgct ggcgacccga cgtagtccat ggatttctgt
     4501 tcgacggaaa cttctactca cgcatcgccg cagtgggcac cgggataccc gttctcaatt
     4561 ccgagcgcag caacggctat gcgctgtcgg ccacgcagcg tctcgcgcac cggctcacgc
     4621 gggcctgtgc gagcggcgtc gtggcaaaca cctactgtgg cgcgcgcttc gcgcaaagcc
     4681 tcttcgggct tccggacgag cgcatccatg tcgtgtggaa cggcatacgg atcgaggagc
     4741 tggagcggca gcggagcact ggcgtcgatt atcggctgga attcttccat gagcgcaatg
     4801 tacgactggc ctgtctcgtg ggttcgatcc gcccgatcaa ggattatctg ctcgcgcttg
     4861 aaactgcggc ccgcctgatc gaacgcgatc ccgcatggcg cgtgctgctt ataggggaca
     4921 gtctcgcacc gggggcagct tatgcggaag gcgcgcccgc gagctctaac ggctacaaag
     4981 cggaggtggt cgcgcgctac cggcagatcg gcctggagga caagatcagg ttttgcggct
     5041 tgcgcggcga cgtgccggcg atcctgcatc agtgcgatgc gctcttcgtg acttcggtca
     5101 acgagggctt tcccaacgct gtgctcgaag cgatggcgct cggcgtgccg gtggtgagca
     5161 ccgattattc cgatatccga cgcattctcc cgctgcaaga acaggtcgtc ccgacacgcg
     5221 atgcggacgg gatcgcgcgc gcgatccttt gggcacactc gcagcgcgag ctgctcgcgg
     5281 caagacagcg cgactgggtc tgcaaccacg cgacgatgga gcgcgccgcg cgcgccctcg
     5341 aagaggtcta ctccacgtat gccggcctgc gcggagcacg cgcgcaacac gaagcggacg
     5401 gcgcgcccca ggcgtacgca cccgcggtgc aggcggagcg cgagcgatga gcgcgcaaca
     5461 cgatcaggcg cgtgcacaca cgctgcggcg cctgcggcag cgtgcattgt cgctcggcgg
     5521 tgccaacgcg ttcgattaca tcacgcaatt cctgctgcct gtcgtactgg cgcggttcct
     5581 ggacacgctc gggttcggtc agtatcgact gctgtggctc gccactcgga ccgcgctttc
     5641 gattgcgccg ctcgccatgc cggcgagcct gtattacttc ctgccccgct cggatggcgc
     5701 ggaccggcgg ctctacgtca accagacact gttctttctt gccgtgacgg gactcctctc
     5761 cggctgggcc gtcagcagct ggaacccgtg gctgccgcag tcgatgcagg cgctggagca
     5821 gcacggtgca atggtccccg tgttcgttgc attgtggatc ctggcgtcgc tcctcgacat
     5881 gctcccggcg atcgaagaac gcgtctcgtg gcagacctgg gcgatcatcg gtctcgcagt
     5941 gttgcgcacc gttgcgctct ccgtggccgc gatcgtgacc ggcgagttgg gcgcagtcct
     6001 gctcgtcatg atcgccttcg tggccttcaa gctcgcgctc cttggtatct acattgcgaa
     6061 gtatcatgga ttgcggggac cgatcgtgcg ctggaaagcg ttcgctcagc agatgaagca
     6121 cgcgacgccg ttcggtgccg caggtgcgct ctacgatctt cgcgcccagg cggatcaatg
     6181 gatcgccgca accctgttct cgctgcatct cttcgccgcg ttttcggtgg gcgcggtgat
     6241 tggaacgctc gtgtcgattt tccgcaagtc ggtgaacacc gcctttctgc cggggatgag
     6301 caggctcgaa gcggcgggcg atctcaatgg catgctggaa ctcaacagcc gcgcgaacgc
     6361 gatggtaggc gcgctcgtgt gcccgctggc cgcgttcgcg tttgtctacg ccgaggaagt
     6421 cgtgacgctc atctataccg aggcctatat ttcggcggcg ccagtcatgc gcgtctacat
     6481 tatcggtctt cttgcactca tcgtagagct cgccagcatc acgctgctcc tgcgccaggg
     6541 gccgttcgtc gtccgcgtca acatcgcggc gctcgtgatc gccattcccg tgagctggct
     6601 tgcggctcgg cactacgggc tcgcgggcgc cgcagccgga agcgtgatcg cgatctacct
     6661 cgatcgcctc gttacgctca aacacatttc gcgactttcc ggcgtaccgc tgcgccggct
     6721 gcaggactgg aacgcgctcg cgacgctgct ggtgtattcc atgctgtccg ccgcgctggc
     6781 gtggtgggtc accgcatcct acttcccgga gccggccccc ttcatcagac tcgtggtagg
     6841 cggctcgacg cttggcgccg cgtacctgct gcttctcaac ataggaaatt tcatgaacgg
     6901 ccttgcggtc gccggcaagc gctagcaccg tagcggacaa gaggatatat cgggaccgcg
     6961 cgcactccgc tgcgcgggcc ccatccggac tatccctcgg ctcaacgtgg aaacacagag
     7021 catgccgaaa atcgccctgt tttccctcgc agcctccgtg tttttgccaa gtgccgaaa
//
LOCUS       DASURG010000208         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_650968, whole genome shotgun sequence.
ACCESSION   DASURG010000208 DASURG010000000
VERSION     DASURG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650968"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..3049
                     /locus_tag="VFY80_03510"
     CDS             80..3049
                     /locus_tag="VFY80_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6007002.1"
                     /translation="MQVLGHVNGLSQEAIEALPVAVYMTDADGRVTFYNEAAAELWGC
                     RPELGESRFCGSWKLYWPDGTPLPHEECPMALSLRQRLPIHGMEAVAERPDGTRVPFI
                     PYPTPLFDESGKLSGAVNMLVDISDRKRAEAALAERKAQLAVFVEHAPAGIAMFDCEM
                     RFLAVSRRLVVDHRLPQDAQLIGRSVYEVFPRLPKRWCDIHARVLAGEKLSQEEDPFT
                     HEDGRTDWMRWSMVPWRRADGNIGGALLFAELRTEQVEARRALADSEARFRATFESAA
                     VGIALVGRNGSILRANDSFVRMLGYSAEELRTKTFQELTYPDDLALNLTVLKKALVGE
                     TDTYCIEKRYVRKDGGVVWASLSVGCVRKPDGRVDYFISVIEDITDRKHAEARLAERN
                     AQLGLAGRAARVGSFVVDYATERIQTSPGFAAIHGLADETEEFTCEEWLGLVLPADLA
                     RFEALRNRVFAERRRELNIDYRVVGTDGEPRWIESRGLVSYGGDGRPTRLVGVHIDIT
                     ERKRAEDRLQESERKLREILGALPAAIYMTDAEGHITYCNQGAVDLWGLEPVLGRDKW
                     SDLGKFYHADGSPMDLAHCPTEVALRQGVITRGQEAIIERKDGVRIPIAPYPTPLRDE
                     TGRIVSVVNMTVDISERKKAERTLAERNLQLALAGKAALVGSYAYDVGSDVMQVEEGY
                     AALHGLPDGTRSTTRDELRARVHQEDLARVEMGLSQAFASGRSEYGFEYRIVRPDGEV
                     RWIESRSFVSSNSDGRQKRVVGVNIDITDRKLAEEHLRMMVAELDHRVKNVLATVSAL
                     TSCTQDASGSVADFVATLDGRIRSMAATHQLLSCQQWKGILLSDLARRELAPYATGNN
                     TEIDGPNIILKAEAAQAVSMVFHELATNAAKHGALSTPGGRVAVRWYRLQNSDADALR
                     VEWQEVGGPVVKAPRRCSYGTEIIRGLLPYELDGKVDLAFAAAGVRCRFDVPLSQLTG
                     CDRLARTPREPLTNGQG"
     gene            complement(3123..3506)
                     /locus_tag="VFY80_03515"
     CDS             complement(3123..3506)
                     /locus_tag="VFY80_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007003.1"
                     /translation="MSRVPEAVRSEDLRGIRVLVVEDQWHVANALRSLLQVEGMEVSG
                     PAGRIADARRLADEYKPELAVIDINLRGEMAYSLIDDLHDRGIRVVVISGYAVLPRLT
                     AKVDAVLQKPFNGPELVASLRRALA"
     gene            complement(3552..>3797)
                     /locus_tag="VFY80_03520"
     CDS             complement(3552..>3797)
                     /locus_tag="VFY80_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6007004.1"
                     /translation="KHGLAGRPKVTIRVGMRREAGGYIFTVEDDGPGFVLQPEHARSL
                     GLGLVMALTRQLNGTFDVERGPGARCILRFPDPRTLN"
BASE COUNT          732 a         1174 c         1212 g          679 t
ORIGIN      
        1 atctgtgcag gttcataccg aagttatccg atggagttca ccactgagta gggttggggc
       61 actaccgggg gccacgcgag tgcaggtcct gggccatgtc aacggtttgt ctcaggaggc
      121 cattgaggcg cttcctgtcg cggtctatat gacggacgct gacggccgcg tcacctttta
      181 caacgaggct gcggccgagc tttggggctg ccggcccgag ctcggcgaga gcaggttctg
      241 cggttcttgg aaactctact ggccggatgg cacgccgctg cctcacgaag aatgtccgat
      301 ggccttgtcg ctgcgtcagc ggcttccgat tcacggcatg gaagcggtag ccgaacgccc
      361 ggacggcacg cgggtgccct ttattcctta tccgaccccg cttttcgatg aatccgggaa
      421 gttgagcggc gccgtcaaca tgctcgttga catcagcgac cgcaagcgcg ctgaagcggc
      481 cctcgcagaa cggaaggcgc agctggcggt gtttgtcgag cacgcaccgg cagggatcgc
      541 catgttcgac tgcgagatgc gcttcctcgc cgtctcgcgt cgattggtcg tcgatcaccg
      601 tcttccccaa gacgcacagc tcattggccg gtcggtctac gaagtctttc cacgactccc
      661 gaagcgctgg tgcgacattc atgcccgcgt gctggccggc gagaagttgt cgcaggagga
      721 agacccgttc acgcacgagg acggccgcac agactggatg cgctggtcga tggtgccgtg
      781 gcgccgagcc gatggcaata tcggcggggc gctcctgttc gctgagctca ggacggagca
      841 ggttgaggcg cggcgcgcgc tcgccgacag cgaggcacgg ttccgggcga ctttcgagag
      901 tgcggcggtg ggcattgcgc tggtgggtcg caacggctca attctgcgtg ccaacgacag
      961 tttcgtccgg atgctgggtt attccgccga ggagctcagg acgaagacct tccaggagct
     1021 cacatacccg gacgatcttg cgctcaatct taccgtgcta aagaaggcgc ttgtcggcga
     1081 gacagacacc tactgcatcg aaaagcgcta tgtccgcaag gacggcggcg tggtctgggc
     1141 aagtctcagc gtcggctgcg tgcggaagcc ggatgggcgc gtcgactact tcatttcagt
     1201 catcgaggac atcacggacc gcaagcatgc cgaggctcgg ctcgcggaac gcaatgcaca
     1261 actcggactc gccgggagag ccgcccgcgt aggcagcttc gtcgtcgact acgctacaga
     1321 acggattcaa acctcgccgg gcttcgccgc cattcacggc ttggcggacg aaaccgaaga
     1381 gtttacctgc gaggaatggc tgggccttgt gcttcccgcc gatctcgcgc gctttgaagc
     1441 gctccgcaac cgagttttcg ccgagcgacg ccgcgagctc aacatcgatt atcgcgttgt
     1501 cggcacggat ggcgagccgc ggtggatcga atcgcgtggc ctcgtttcct atggcggcga
     1561 cggacgcccg acgcgcctgg tcggcgtcca tatcgatatc acagagcgaa aacgggcaga
     1621 ggacaggctg caggagagtg agcgcaagct gcgcgagatc ctcggtgcat tgccggcagc
     1681 aatctacatg acggatgcag agggtcacat aacctattgc aaccagggcg ccgtcgatct
     1741 ctggggattg gaacccgtac tgggaaggga caagtggagc gacttgggaa agttctatca
     1801 tgccgacggc tcgccgatgg acttggcaca ctgcccaact gaggtcgcac tgagacaagg
     1861 cgtgatcacg cgcggacaag aagcgatcat cgagcgcaaa gacggcgttc gcatcccgat
     1921 agccccgtat ccgacaccgc tgcgcgacga gaccggcagg attgtcagcg tcgtgaacat
     1981 gaccgtcgac atcagtgaac gcaagaaagc cgagcgcacc ctcgccgaac gcaatctgca
     2041 gctggcactg gccgggaagg cggccctcgt cggcagctac gcctacgacg taggctcgga
     2101 tgtgatgcaa gtcgaggagg gttatgcggc cctgcatggc ctgcccgacg gaaccaggtc
     2161 cactacgcgg gacgaattga gagcccgagt gcaccaggag gaccttgccc gggtggagat
     2221 gggcctcagc caagcgttcg ccagcgggcg aagcgagtat ggcttcgaat accgaattgt
     2281 gcggcctgac ggcgaggtcc gctggatcga gtcgcgcagt ttcgtttcgt ccaacagcga
     2341 cgggcgtcag aagcgtgtgg ttggcgtcaa catcgacatc acggatcgca agctggccga
     2401 agaacatctg cgcatgatgg ttgccgagct cgatcatcgc gtgaagaacg tccttgcaac
     2461 tgtcagcgct ctcacatcct gtacgcagga cgcgagcggc tctgtggccg acttcgtggc
     2521 cacgctcgac ggccgaatac ggtccatggc ggcaacgcat cagcttttga gctgtcagca
     2581 gtggaagggc atactgctgt ccgacctcgc tcgccgcgag cttgctccct acgcaaccgg
     2641 caacaacacc gagatcgacg gcccgaacat aatcctgaag gcggaagccg ctcaggcggt
     2701 ttcaatggtg tttcacgagt tggcgaccaa cgccgcaaag cacggcgcgc tttcgacccc
     2761 gggcggccgg gttgctgtac gctggtatcg attgcagaac agcgatgcgg atgcgctgcg
     2821 cgttgaatgg caggaagtcg ggggcccggt tgtcaaagct ccgcgcagat gtagctatgg
     2881 gacggaaatt atccgcggtc ttctgcccta cgagctcgat ggcaaggttg acctggcttt
     2941 cgccgccgcg ggcgtcaggt gccggttcga cgttccgctc tcccaactta ccggctgtga
     3001 ccggctagcc aggacgcccc gtgaaccgtt gaccaatggc caaggctgag caacgcagcc
     3061 tcctggacgc aaagccgtca ttccggcttt cgctcggaac ctgccggagc ccgcggacag
     3121 ctctacgcga gcgcacgacg caatgacgcc accaactccg gtccattgaa cggcttctgc
     3181 aggacggcat cgaccttcgc cgtcagtcgc ggcagcacgg catagccgga gatcaccacc
     3241 acgcgaatcc cccggtcgtg cagatcatcg atgagggaat aagccatctc gcctctgaga
     3301 ttgatgtcga tgactgccag ctccggcttg tactcatcgg cgagccggcg ggcgtcggcg
     3361 atcctgccgg ctggcccgct tacctccatg ccttcaacct gcaggagcga tctcagcgca
     3421 ttggcaacgt gccattgatc ctccacgacc aggacacgaa taccccgcag gtcttccgac
     3481 ctcaccgcct ccggaacgcg cgacattggc aaatggcctc cttcaaagca ccgcgcaggc
     3541 cgccggacgc ttcaattcag tgtgcgcggg tcagggaaac gcaaaatgca gcgggcgccc
     3601 gggcccctct ccacgtcgaa agtgccgttc agctgacggg tcaacgccat cacgaggcca
     3661 aggccgagcg agcgcgcgtg ctcgggttgg agcacaaacc ctgggccgtc gtcctcaacc
     3721 gtgaagatgt agccgccggc ttccctccgc attccgacgc ggatggtgac tttgggtcgg
     3781 ccggccagcc cgtgctt
//
LOCUS       DASURG010000209         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1161109, whole genome shotgun sequence.
ACCESSION   DASURG010000209 DASURG010000000
VERSION     DASURG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161109"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFY80_03525"
     CDS             complement(<1..505)
                     /locus_tag="VFY80_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6007005.1"
                     /translation="MKVDPNRIRCIDPLVHPQYPYNETPRTPERSEPRYASISDYEAR
                     IPLRDGITLAADVIRPHAGGMKFPALVTTSVYTRQLQRSVIALGQNEAGVSEFWVPRG
                     YAHVIVDVRGCNDSEGEYDLFGAQEQQDLYDIIEWVAAQPWCDGNVGMSGISYMGRTQ
                     LFAAEQQP"
     gene            complement(610..1155)
                     /locus_tag="VFY80_03530"
     CDS             complement(610..1155)
                     /locus_tag="VFY80_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6007006.1"
                     /translation="MKNRYGTTGVAAAFVLALAAPTVIASDLGTAVGGGLGAAGGAAV
                     GQAIGGKNGSIVGGAVGGAVGGAATTNGEGKTGAMVGGAVGGAAGAAVGQSVGGKSGA
                     VVGAGAGGAAGAVIGKNVSEGSGGAASAPVATQGRTVRVYQPQPRVVVTTGYYDDDPH
                     CGKRKHKNHPGKGHAKGHYKC"
     gene            complement(1332..1550)
                     /locus_tag="VFY80_03535"
     CDS             complement(1332..1550)
                     /locus_tag="VFY80_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007007.1"
                     /translation="MHDTVEFEKRGIPATVVITQAFRNAAEFQFRGRGMEGHPYIELP
                     HPISNLKPEEMKALALRYVDDVVRQLTA"
     gene            complement(1572..1862)
                     /locus_tag="VFY80_03540"
     CDS             complement(1572..1862)
                     /locus_tag="VFY80_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007008.1"
                     /translation="MADEMNFIDPTAGGDEAKILRAPRPMDLAGKVVGLLDNTKEQAD
                     IILATLGEALRERHGVARIVIRRKEHYSKPAAPAIIADMAKEVEVAIAAVGG"
     gene            complement(1989..>2667)
                     /locus_tag="VFY80_03545"
     CDS             complement(1989..>2667)
                     /locus_tag="VFY80_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007009.1"
                     /translation="LHGGNGCFGPGFRANASIGRAIRLMMTNLGGGIPGVASATIFAT
                     PLRYTACITENIERSPWETLAVSRSYSPEDDVITCAMVESPRLCFDDVSQDPGRLLAG
                     LASSMADICSWNMHARSDMVVAMGPQHATICANAGLTRADVHRALLEKAGRRVRELKV
                     GGNWRRERALALPIEVDPDDDDFFVPAIKAPQDLHLIVAGGWGPCTAMCHGWSGGSRA
                     VHGTYET"
BASE COUNT          421 a          925 c          825 g          496 t
ORIGIN      
        1 gcggttgctg ctccgcggcg aagagttgcg tgcggcccat gtaggagatg ccgctcatgc
       61 cgacgttacc atcgcaccac ggttgcgccg cgacccattc gatgatgtcg tagaggtcct
      121 gctgctcctg cgccccgaaa agatcgtatt cgccttccga atcgttgcac ccgcgcacgt
      181 ccacgatcac gtgcgcgtag ccgcgtggaa cccagaattc cgagacgccg gcttcgttct
      241 ggccgagcgc gatgaccgag cgctggagct ggcgcgtata gactgaagtc gtcaccagcg
      301 cgggaaactt catcccgccg gcatgagggc ggatcacgtc ggcggcgagc gtgatgccgt
      361 cgcgcagcgg aatgcgcgct tcgtaatccg agatcgaggc gtagcgcggc tccgagcgct
      421 ccggcgtgcg cggcgtttcg ttgtacggat actgcgggtg gacgagcgga tcgatgcagc
      481 ggatgcgatt cggatcgact ttcatgccgg ggctcctcgc ctgggatcgg atgtcggggc
      541 cgctagctta gcaaagaaaa aggcccgctc gcgcgggccc atccctccct gtttcccctt
      601 cttattgttt cagcacttgt agtgtccctt ggcgtggcct ttgccgggat gattcttgtg
      661 cttgcgcttt ccacaatgcg gatcgtcgtc ataatatccc gtcgtgacga cgacgcgcgg
      721 ctgcggctga tatacacgca cggtgcggcc ctgggtcgcg accggcgcgg acgccgcccc
      781 gcccgatcct tcgctcacgt tcttgccgat caccgcgccg gccgcgcctc ccgcgccggc
      841 accgactacc gcacccgatt tgccgccgac cgactggccg accgcagcac cggccgcacc
      901 acctacggcg ccgcccacca tcgcgccggt cttgccctcc ccattcgtcg tcgcggcccc
      961 tccgacggct ccgccgaccg cgccgccgac gatggaaccg ttcttgccgc cgatcgcctg
     1021 ccccacggcc gcaccgcccg cagcgccgag ccccccaccg acggccgtcc cgagatcact
     1081 tgcgattacc gtcggggccg cgagcgccag gacaaatgca gccgccaccc ctgtcgttcc
     1141 gtacctgttc ttcatttttt cgttctcctt tgcgcggctg cgtgcaggca ggcggcgcac
     1201 atccgctgcg catcgtgcac gaagcggggc ggaaccgctg tgaacagcat cacgaatttc
     1261 gggcggagcg cggggagagc ggcactattt catgcttcgg ccgcttcgtt ctccataacg
     1321 ctgtttcgaa atcaggcggt aagctggcgc accacgtcat cgacgtaacg cagcgcgagc
     1381 gccttcattt cctcgggctt gagattgctg atcgggtgcg gcaactcgat gtagggatgg
     1441 ccctccattc cccggccgcg aaactggaac tcggcggcat tgcggaacgc ctgcgtgatg
     1501 acgacggtgg cagggatgcc gcgcttttcg aattcgacgg tgtcgtgcac actgcacaac
     1561 gtgcaggagc ctcacccgcc gacggccgcg attgcgacct cgacctcctt cgccatgtcg
     1621 gcgatgatcg cgggcgccgc gggcttggag tagtgctcct tgcgcctgat gacgatgcgc
     1681 gcgacgccgt gacgttcgcg cagcgcttcg cccagtgtcg caaggatgat atcggcctgc
     1741 tctttggtat tgtccagcag tccgaccact ttgcccgcga gatccatcgg gcgcggcgcg
     1801 cggaggattt tcgcctcgtc gccgcccgcc gtcgggtcga tgaagttcat ttcgtcagcc
     1861 atcgctactc ctttttacgc atcgttattc cacatctcgt ccggaacgtt ctctggaccc
     1921 cggcttccgc cggggagacg atcgtcggat taaatccctc cgagcaagct ctcaacgacg
     1981 ggacgacgtc aagtctcata cgtgccgtgg acggcgcggc tcccgccgct ccagccgtgg
     2041 cacatcgcgg tgcacggccc ccagccgccc gcgacgatga gatgcagatc ctgcggcgct
     2101 ttgatcgccg gaacgaaaaa atcatcatcg tccggatcga cctcaatggg tagcgccagc
     2161 gcccgctcgc gccgccagtt gccgccgacc ttcagctcac gcaccctccg ccccgccttt
     2221 tcaagcagcg ctcgatgaac gtcggcgcgc gtcaggccgg cgttcgcgca gatcgtcgcg
     2281 tgctgcgggc ccatcgccac caccatgtcg gagcgggcgt gcatgttcca cgagcagata
     2341 tcggccatcg aacttgcgag tcccgcgagc agccgaccgg ggtcctggct cacgtcgtcg
     2401 aagcagaggc gcggactttc caccatggca caggtgatca catcatcctc gggtgaataa
     2461 ctgcgcgaga cggcgagcgt ctcccacggg ctgcgctcga tgttctcggt gatgcacgca
     2521 gtgtagcgca gcggcgtcgc gaatatggtg gcggaggcga cgcccgggat gccgcccccg
     2581 agattcgtca tcatgagccg gatcgcgcgc ccgatcgaag cgttggcgcg gaaacccgga
     2641 ccgaagcagc cgttcccgcc gtgcagt
//
LOCUS       DASURG010000210         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_139615, whole genome shotgun sequence.
ACCESSION   DASURG010000210 DASURG010000000
VERSION     DASURG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139615"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1013
                     /locus_tag="VFY80_03550"
     CDS             441..1013
                     /locus_tag="VFY80_03550"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX6007010.1"
                     /translation="MLLMIDNYDSFTYNLVQYCGELGEDVRVVRNDEVTLDEIEAMRP
                     ARIILSPGPCTPNEAGVSVPLVKRFAGKTPILGVCLGHQSIGQAFGARIVHAQRVMHG
                     KTSAIEHTGAGVFRGLPNPFHATRYHSLAIERSTLPESLEVTASSEDGEIMGVRHKRL
                     AVEGVQFHPESILTEHGHALLKNFLEVDRT"
     gene            1010..2029
                     /gene="trpD"
                     /locus_tag="VFY80_03555"
     CDS             1010..2029
                     /gene="trpD"
                     /locus_tag="VFY80_03555"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX6007011.1"
                     /translation="MTPQEALTRVIEHREIFREEMLALMRSIMTGEVSAPLIAAIITG
                     LRVKKETIGEISAAAEVMREFATKVPVPDEPRLIDTCGTGGDGAHSFNISTASMFVAA
                     AAGARIATHGGRSVSSTCGSADVLEALGANINLEPERVARSISEVGVGFMFAPNHHKA
                     MKYAAPVRKELGVKTIFNILGPLTNPAGARQQVMGVFHPDLVGIQARVLQRLGSRRVL
                     VVHGLEGLDEISVSGPTMVGELKDGEVHEYTIAPQDFGLPTHDASAIRVQGVDQSKGM
                     LLAALEDQPGAPRDVVALNAGASIYVAGVTETLREGVVLARSIIASGAARKRLDQFVT
                     FTRAG"
     gene            2041..2838
                     /gene="trpC"
                     /locus_tag="VFY80_03560"
     CDS             2041..2838
                     /gene="trpC"
                     /locus_tag="VFY80_03560"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX6007012.1"
                     /translation="MADILRRILEAKASEVAEARSRTPLVALESAARKAAEVRDFVGA
                     LRHRISSGFPAVIAEIKKASPSRGVLRADFAPDALASDYARHGATCLSVLTDAQFFQG
                     RLEDLVRARAACALPVLRKDFMIDPYQVLESRAAGADCILLIVAALDTPRLRELESVA
                     RAWGLAVLAEVHDGAELDRALELETPLIGVNNRNLRTFETRLETTLELVNRIPQDRLV
                     VTESGILAPEHVQRMRKHNVHGFLVGEAFMRAAEPGAELERLFGKAA"
     gene            2847..3470
                     /gene="coq7"
                     /locus_tag="VFY80_03565"
     CDS             2847..3470
                     /gene="coq7"
                     /locus_tag="VFY80_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX6007013.1"
                     /translation="MDFDRLIVAFDRGLRSLFAPAQSLRPVPGEALADAVLNESEKRE
                     AAALMRVNHTGEICAQALYQGQALTARDTQAKAALEQAAREETEHLAWTERRIEELGG
                     RKSLLNPFWYAGSFVMGAAAGTLGDRWNLGFLAETERQVVEHLDGHLRRLPLEDLKSR
                     AVIEQMKEDEGRHATSAREHGGTELPDPVRFAMRCASWVMTRTAFWL"
     gene            complement(3479..>3576)
                     /locus_tag="VFY80_03570"
     CDS             complement(3479..>3576)
                     /locus_tag="VFY80_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6007014.1"
                     /translation="AIQLSAEKYCSASIMLGKTADITHDFEIRDA"
BASE COUNT          668 a         1125 c         1157 g          626 t
ORIGIN      
        1 gccgccccga gtaccagggc caacgcgagt cccgccgagg tggatcggcc ccgtcggtac
       61 ctcgtataca catcctgaca ctgaggcctc gacgccgaaa ggatcggcac gttacaaatt
      121 ccccttttac gcgcggagtg agaggcaagc gtgcgcattc gcgtggcgcg cgcccggatc
      181 cggccagccg tccgaatgct accagtcccc atgcgggcgg agcaatcgcg gcacaggcgg
      241 ctctcgaatg attcaggtaa aattttcaca tgtcgcgttc cgcttgtcgg caaagcctcc
      301 cccgctcgca tcgcgctgcg cgatggcgat agaccgcacc gctccgcccc gttcccggct
      361 ccgaaagact cgcagggtgc ttgcagaccg gccggttctt gcacggcccg cccaaccttg
      421 gacgcgatac aggtgtaccg atgctgctca tgatcgacaa ttacgactcc ttcacctaca
      481 acctggtgca gtactgcggg gagctcggtg aagacgtgcg cgtggtgcgc aatgacgaag
      541 tcacgctcga cgagatcgag gcgatgcggc ctgcccgcat catcctctct ccgggcccgt
      601 gcacgcccaa cgaagccggc gtgtcggtgc cattggtcaa gcgctttgcc ggaaaaacgc
      661 cgatactcgg ggtctgcctc ggccatcaga gcataggaca ggcgttcggc gcgcgtatcg
      721 tgcacgcaca gcgcgtgatg cacggcaaga cctcggccat cgagcatacg ggggcgggag
      781 tcttccgcgg attgcccaat ccgtttcatg cgacgcgcta ccattccctg gccatagagc
      841 gctccacgct gccggaaagc ctggaagtca ccgcctcttc cgaggacggc gagatcatgg
      901 gtgtccggca caagcggctc gcggtcgaag gcgtccagtt tcatccggag tcgatcctga
      961 cggagcacgg tcatgcgctg ctcaagaact ttctggaggt agaccgcaca tgacgccaca
     1021 ggaggcactg acccgtgtca tcgagcatcg cgagatcttc cgcgaagaaa tgctcgcgct
     1081 catgcgcagc atcatgaccg gagaagtgtc cgctccgctg atcgcggcca tcatcaccgg
     1141 actgcgcgtc aagaaagaaa cgatcggaga gatttcggcg gcagccgaag tgatgcgcga
     1201 gttcgccacc aaggttcccg tcccggacga gccgcggctc atcgatacct gcggcacggg
     1261 cggggacggc gcgcattcct ttaacatttc gaccgcttcg atgtttgtag ccgcggccgc
     1321 gggcgcgcgt atcgcgacgc acggcggacg atcggtgtcg agcacgtgcg gcagcgccga
     1381 cgtgctggaa gcgctcggcg ccaacatcaa tctcgagccc gagcgggtcg cacgctcgat
     1441 ctcggaggtc ggcgtcggct tcatgttcgc accgaatcac cacaaggcaa tgaaatacgc
     1501 tgcgccggtg cgcaaggagc tgggcgtcaa gacgatattc aatatcctcg gtccgctcac
     1561 caatcctgcc ggcgcccgcc agcaggtgat gggcgtattc catccggatc tcgtcggcat
     1621 ccaggcgcgc gtgctgcagc ggctggggag ccgccgtgtc ctcgtcgtgc acggattgga
     1681 gggactcgac gagatttcgg tgagcgggcc cacgatggtg ggcgaattga aggacggcga
     1741 agtacacgaa tacaccatcg caccacagga tttcggcctg ccgacgcacg atgcgagtgc
     1801 catacgcgtg caaggtgtgg accagtccaa gggcatgctg ctcgctgcgc tcgaagacca
     1861 gcccggcgcg ccacgcgacg tggtggccct caacgccggc gcgagcattt atgtggccgg
     1921 cgtgacggaa acactgcgcg aaggcgtcgt cctcgcccga tccatcatcg cgagcggagc
     1981 cgcgcgcaag cggctggacc aattcgtaac tttcacgcgg gcgggataga cgcgcgcatc
     2041 atggccgata tcctcaggcg cattctcgaa gccaaagcga gcgaagtggc ggaggcacgg
     2101 agccgcacgc cgctcgttgc gctcgaatcc gctgcacgta aggctgcaga agtgcgcgac
     2161 tttgtgggcg cgttgcgcca caggatctcg agcggctttc cggcagtcat tgccgagatc
     2221 aagaaagcga gcccaagccg cggcgtcttg cgcgccgatt tcgcgcccga tgcgcttgca
     2281 agcgattacg cgcgccatgg cgcgacgtgc ctgtcggttc tgaccgatgc gcagtttttt
     2341 cagggtcgtc tagaagacct ggtgcgcgcc cgtgccgcat gcgcgctccc ggtcctgcgc
     2401 aaggatttca tgatcgaccc ttaccaggtg ctggaatcgc gagcggcggg cgccgattgc
     2461 atactgctca tcgtggcggc gctcgatacg ccgcggctgc gcgagctcga atcggttgcc
     2521 cgcgcctggg gccttgccgt tctcgccgaa gttcacgacg gcgccgaact ggaccgcgcg
     2581 ctggaactcg agacgccgct catcggcgtc aacaatcgca acctgcgcac gtttgagacg
     2641 cggctcgaaa ccacgctgga gctcgtcaac cgcatcccgc aggatcggct ggtggtgacc
     2701 gaaagcggga tactcgcgcc tgagcatgtc cagcgcatgc gcaagcacaa tgtccacggc
     2761 tttctggttg gggaagcttt tatgcgtgcc gccgaaccgg gcgctgaact ggaacggttg
     2821 ttcggaaagg cggcgtgagg cgcgctatgg atttcgatcg cctgatcgtc gctttcgacc
     2881 gcggacttcg ctcgctgttt gcgccggcgc aatctctacg cccggttccc ggcgaggcgc
     2941 tcgctgatgc ggtgttgaac gaaagcgaaa aacgcgaggc ggccgcattg atgcgcgtca
     3001 atcataccgg ggagatctgt gcgcaagcgc tttatcaggg acaggcgttg accgctcgcg
     3061 acacgcaggc aaaggcggcg ctcgaacagg cggcacgcga ggaaacggaa cacctcgcat
     3121 ggaccgagcg ccgcattgaa gagctgggcg gaagaaaaag cctgctcaat ccgttctggt
     3181 acgcggggtc gtttgtcatg ggtgccgccg ccgggactct cggcgatcgc tggaacctgg
     3241 gttttctcgc cgaaaccgaa cggcaggtgg tggaacacct cgacggtcat ttgcgcagac
     3301 tcccgctcga agatctgaaa agccgcgcgg tgatcgagca gatgaaggaa gacgaggggc
     3361 gccatgcgac gagcgcccgc gagcatggcg ggaccgagct gccggatccc gtgcgcttcg
     3421 ccatgcgctg cgcgtcctgg gtgatgaccc gcaccgcatt ctggctttaa agtaggactt
     3481 aagcgtcgcg aatttcgaag tcgtgggtga tgtccgcggt cttcccgagc atgatggacg
     3541 ccgagcagta tttttctgcc gagagctgta tcgccc
//
LOCUS       DASURG010000211         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1263148, whole genome shotgun sequence.
ACCESSION   DASURG010000211 DASURG010000000
VERSION     DASURG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263148"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1820
                     /locus_tag="VFY80_03575"
     CDS             3..1820
                     /locus_tag="VFY80_03575"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007015.1"
                     /translation="MILWLAAALLLQQPTQSAEINYEISFPNAAHHEAQVSVTFTRLP
                     ARPLELRMSRSSPGRYALHEFAKNVYEVKAFDADGQPLAVTRPNEHQWNVPRHKGHVR
                     VTYTLFGDRTDGTYAGIDRTHAHLNIPATFMFARGLQDRPIRVRFNLPDPAWRVATQL
                     APTLDPHVFTAPHLQYFMDSPTELSRHVVRSWTVPTGTRADTIRLAIHHTGGDAEVDT
                     FVERIKQVVLEQRAVYGELPRFDYGSYTFIADYLPWANGDGMEHRNSTILTSSTSLTQ
                     NATGLLGTVAHEFFHAWNVERLRPRSLEPFDFERANMSDALWFAEGFTSYYGPLTLKR
                     AGLTSLDDYARALSGVVNTVLTAPGRQYFSPVEMSMQAPFVDAAASIDPHNRTNTFIS
                     YYTYGAALALGLDLLLRTEHTYVTLDDFMRAMWTRYGRPQTPYTLGDWRTTLGQVVRD
                     QPWADRFFHDHVAGTQPIAFAPLLARAGLLLRKAAPGRATLGAVQFAPDSTRVLIAGS
                     TTVGSPLYRAGVDRGDHIVSLDGRVLRAAADIAGILVTHKPGETIPIVFESRGVTVNG
                     NVTLGEEERLEVVTYEAAGMTVTDEMKTLREAWLGPKAR"
     gene            complement(1927..>2248)
                     /locus_tag="VFY80_03580"
     CDS             complement(1927..>2248)
                     /locus_tag="VFY80_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6007016.1"
                     /translation="DLLRIAKNDNVARETRKSAIFWVGQEVAEEATRHLKDVVTDRGD
                     LEVRKSAIFALSQQRTGDAVGALIEIARTNPEKELRKSAFFWLGQSDDPRVMAMFEDI
                     LLRN"
BASE COUNT          390 a          722 c          711 g          425 t
ORIGIN      
        1 caatgatcct atggttggcc gcagcgctgc tgttacagca accgacgcaa tccgccgaga
       61 tcaattacga gatctcgttt ccgaatgctg ctcaccatga agcgcaggtg agtgtgactt
      121 tcacacggtt gccggcgcgc ccgctggagc tgcgcatgag ccggtcgagc ccggggcgtt
      181 acgcgctgca cgagtttgcc aagaacgttt acgaggtgaa agcgttcgat gccgatggcc
      241 agccactcgc ggtcacacgt ccgaacgaac accagtggaa cgtgccgagg cacaaggggc
      301 acgtgcgtgt cacctacacg ctttttggtg atcggacaga tggtacgtac gcgggcattg
      361 atcgcacgca cgcgcacctg aacatcccgg ccaccttcat gtttgcgcgc ggtctgcagg
      421 accggcccat tcgagtgcgc ttcaacctgc ccgatcccgc ctggagggtt gcgacgcagc
      481 tggcgcctac gcttgatccg cacgtcttta ccgcgccgca tttgcagtat ttcatggaca
      541 gcccgacgga gctgagccgt cacgtggtgc gcagctggac ggtgccgaca ggaacgcggg
      601 cggacacgat ccgcctggcg attcatcaca cgggtggcga tgcggaagtc gacacgttcg
      661 tcgaacggat caagcaagtg gtgctcgagc agcgtgcggt gtacggcgag ctgccccgct
      721 tcgattatgg cagttacacg ttcatcgcgg actatctgcc ctgggccaac ggcgatggca
      781 tggagcatcg caactcgacg attctgacgt cgagcacatc tctcacgcag aatgcgaccg
      841 gcctgctcgg tacggtcgca cacgagttct tccatgcctg gaatgtcgag cgtttgcggc
      901 cgcgctcgct ggagcccttc gacttcgagc gcgccaacat gtcggacgcg ctctggttcg
      961 cggaaggctt caccagctat tacggaccgc tgactctcaa gcgtgcgggt ttgacctcgc
     1021 tggacgatta tgcccgcgcg ctcagcggtg tggtcaacac cgtgttgacg gcgccggggc
     1081 ggcaatattt cagcccggtc gagatgagta tgcaggcacc cttcgtcgac gccgcggctt
     1141 ccattgaccc gcacaatcgc accaacactt tcatctctta ctacacgtac ggcgcagccc
     1201 tcgcgctcgg gctggacctg ctgctgcgca ccgagcacac gtatgtcacg ctcgatgatt
     1261 tcatgcgtgc catgtggacc cgctacggtc gcccgcagac gccctacacg ctgggtgact
     1321 ggcgcaccac gctcgggcag gtggttcgcg accagccctg ggccgaccgc ttcttccacg
     1381 accatgtcgc gggcacgcag ccgatcgcct ttgcgccgct gctggcgcgt gcgggactgc
     1441 tgttgcgcaa agccgcgccc ggccgcgcta cgctgggcgc cgtgcaattc gcgcccgact
     1501 ccacccgcgt gctcatcgcc ggcagcacaa cggtcggcag cccgctctac cgagccggcg
     1561 ttgatcgcgg ggatcacatc gtttcgttgg acggccgcgt gctgcgcgcg gctgccgaca
     1621 tcgccgggat cctggtcacg cacaagccgg gcgagaccat tcccattgtc ttcgaatcac
     1681 gtggggtcac ggtcaacggc aacgtgacgc tgggtgagga ggagcggctc gaggtggtga
     1741 cgtacgaggc ggccgggatg acggtcaccg atgagatgaa gaccttgagg gaagcgtggc
     1801 tcgggcccaa agctcgttaa ccgcgctcac gggtttcgag cggtcggcgt gggccacgca
     1861 tttcgggttt ctcatcgcgg tgcgttgggg caacgaccgt cgcgagcggc atcgccccgc
     1921 gcagcttcag ttacgcagca gtatatcctc gaacatggcc atgacacgcg ggtcatccga
     1981 ttgaccgagc cagaagaaag cggatttgcg cagctctttc tccggattcg tccgggcgat
     2041 ttcgatgagc gcgccaacgg catcaccggt acgctgctgc gagagcgcga agatggccga
     2101 cttgcggact tccaggtcac cgcggtcggt gacgacatcc ttcaggtgcc gggttgcctc
     2161 ttcggccacc tcctggccca cccagaagat ggccgatttg cgggtctcgc gcgccacgtt
     2221 gtcgttcttc gcaatgcgca gcagatcg
//
LOCUS       DASURG010000212         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_548599, whole genome shotgun sequence.
ACCESSION   DASURG010000212 DASURG010000000
VERSION     DASURG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548599"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFY80_03585"
     CDS             <1..594
                     /locus_tag="VFY80_03585"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX6007017.1"
                     /translation="MVDVSHISKAAMLEAVRVSRAPVIASHSSTRALADHGRNLDDEQ
                     LEALKRNGGVMQTVAFSAYVKVQPPERLAAIRELRQQFGVTSSASLRAMPADRRAEYD
                     RALADIERRWPRATVRDFVDHIDYAVKKIGIDHVGISSDFDGGGGVEGWDNARETFNV
                     TLELVRRGYSEAEIAKLWGGNLLRVWRAVERVAQQGT"
     gene            785..1159
                     /gene="rpsL"
                     /locus_tag="VFY80_03590"
     CDS             785..1159
                     /gene="rpsL"
                     /locus_tag="VFY80_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6007018.1"
                     /translation="MPTINQLIRRGRQAVEKINKSPHLKGNPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGTL
                     DASGVADRHQGRSKYGAKKAKK"
     gene            1175..1645
                     /gene="rpsG"
                     /locus_tag="VFY80_03595"
     CDS             1175..1645
                     /gene="rpsG"
                     /locus_tag="VFY80_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6007019.1"
                     /translation="MSRRQRAVKRETPVDPQYGSATVTKFINSLMLDGKKSVAERIFY
                     DAMRIVEEKTGQPGMNVFKQALNNAKPVIEVKSRRVGGATYQVPVEVRPERRTALAMK
                     WLVNYARDRGDKTMSERLANELLAAARNEGNTIKKKEDTHRMAEANKAFAHYRW"
     gene            1657..>2958
                     /gene="fusA"
                     /locus_tag="VFY80_03600"
     CDS             1657..>2958
                     /gene="fusA"
                     /locus_tag="VFY80_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6007020.1"
                     /translation="MARSTPLHKLRNIGIMAHIDAGKTTTTERILYFTGRIHRVGEVH
                     EGAATMDWMEQEQERGITITSATTTAFWKRFGEDYRINIIDTPGHVDFTVEVERSLRV
                     LDGAVAVFCAVGGVEPQSETVWRQADRYGVPRIAFVNKMDRVGADFMNVVGMIRDRLG
                     AKAFPIHYPLGEGELFTGVVDIIRRVEYVYDDATQGKNWTEGPVPEALRDTIETLRHE
                     LVEAAVETDEELLTKYLEGEEITEDELRRAIRKACIAGTMVPVLCGSAFKNKGVQQLL
                     DAAIDFLPSPLDIPPVQGHLPHHDETIETREADDNAPFSALAFKIMTDPFVGKLTYFR
                     VYSGSLPSGSYIYNSTKDRRERIGRLLQMHANRREEIEEVYAGDIAAAIGLKETRTGD
                     TLCDPNKPIILEAMKFPEPVIEVAIEPKTKADQEKLGAALQK"
BASE COUNT          641 a          865 c          951 g          501 t
ORIGIN      
        1 atggtcgacg tctcccatat ctcgaaggcg gccatgctgg aggccgtccg cgtgtcgagg
       61 gcgccggtca tcgcttccca ctccagtacg cgtgccctgg ccgaccatgg ccgcaacctg
      121 gacgacgagc agttggaagc acttaagcgg aacggcgggg tcatgcagac cgtagccttc
      181 agcgcctatg taaaagtcca gccgcccgag cggctggcgg cgatccgcga gctgcgccag
      241 cagttcggcg tgaccagcag tgcctcgctt cgcgccatgc cggccgaccg gcgcgccgag
      301 tacgaccggg ccctggccga catcgagcgc cgctggccgc gcgcgaccgt ccgcgacttc
      361 gtagaccaca tcgactacgc cgtcaaaaag atcgggatcg accacgtcgg tatcagttcg
      421 gacttcgatg gtggcggggg ggtcgagggg tgggacaacg cccgcgagac gttcaatgtc
      481 acgctcgagc tggttcggcg cgggtactct gaagccgaaa ttgccaagct ttggggtggc
      541 aatctgctca gggtctggcg ggcggtggaa cgggtcgcac aacagggcac gtaaggggcc
      601 atgtcagctt gacataacat catccctcct atacctttcc atgtctatcg gccaattttt
      661 ccggccgctc tatttgtagt aatgggtgac cagaaccggt ccgggggcaa aaactcccgg
      721 ccaaggcggg ggcaagcctt atagctcccg cgtttttact gtcaggaatt cggagaatca
      781 gggaatgccg acaatcaacc agctgatccg gagaggtcgg caagcggttg aaaagatcaa
      841 caagtcgccg catctgaagg gcaatcctca gaagcgcggc gtctgtacgc gtgtgtatac
      901 cacgacgccg aagaagccga actcggcgct gcgcaaggtt gcgcgcgttc gcctcacgaa
      961 cggtttcgag gtaaccgcct acatcccggg cgaaggccac aacctgcagg aacactcgat
     1021 cgtgctgatt cggggcggcc gtgtgaagga cctgccgggt gtgcgctacc acatcattcg
     1081 cggcacactg gacgcgagcg gcgtagccga ccggcaccag ggtcgatcca agtacggcgc
     1141 caagaaagcc aagaaataga gtagctgaga aaccatgagc cgacgccaga gagcagtaaa
     1201 gcgggaaacc ccggtcgatc cccagtacgg atccgcaacg gtcaccaagt tcatcaacag
     1261 cctgatgctg gatggcaaga agtccgtcgc cgagcggatc ttctatgatg cgatgcgcat
     1321 tgtggaagag aagaccggcc aaccgggcat gaacgtgttc aagcaggcgc tcaacaatgc
     1381 caagcctgtg atcgaggtga agagccgccg cgtgggtggt gccacctacc aggtgccggt
     1441 ggaagtgcgg cccgagcggc gcacggcgct ggccatgaag tggctggtca actatgcgcg
     1501 tgatcgcggc gacaagacga tgtcggagcg gctggcgaac gagctgctcg cagctgcacg
     1561 taacgaaggc aacacgatta agaagaagga agacacacac cgcatggcgg aagccaacaa
     1621 ggctttcgcg cattaccggt ggtagtcgac acataaatgg ctagatcgac accgcttcac
     1681 aagctgcgca acatcggcat catggctcac attgatgccg gcaaaacgac gacgacggaa
     1741 cgcatcctgt atttcacggg ccgcattcat cgtgttggcg aggtgcatga aggcgccgcc
     1801 accatggact ggatggagca ggagcaggag cgcggcatca cgatcacgtc ggccacgacg
     1861 accgcgttct ggaagcgttt cggcgaggat taccgcatta acatcatcga cacgcccgga
     1921 cacgtcgact tcacggttga agtcgagcgt tcgctgcgtg tgctcgatgg cgctgttgct
     1981 gtgttctgcg cggtgggtgg tgtagagccg cagtctgaaa ccgtttggcg ccaggccgac
     2041 cgttacggcg tgccgcgcat cgcgttcgtc aacaagatgg accgcgttgg cgcggacttc
     2101 atgaacgtcg tcggcatgat tcgcgaccgt ctgggcgcga aggcgttccc gattcattac
     2161 ccgctgggtg aaggcgagct gtttaccggc gtcgtcgaca tcatccgtcg tgttgaatac
     2221 gtctatgatg acgccacgca gggcaagaac tggacggaag gtccggtgcc cgaggcgctg
     2281 cgcgatacca tcgagacgct gcgtcacgag ctggtcgaag cagccgtcga aaccgatgaa
     2341 gagctgctga ccaagtacct cgaaggcgaa gaaatcaccg aagacgagct gcgccgcgcc
     2401 atccgcaagg cgtgcatcgc gggcaccatg gtgccggtgt tgtgcggctc ggcgttcaag
     2461 aacaagggcg tgcagcagct gctcgatgcg gccatcgatt tcctgccctc accgctggac
     2521 attccgccgg tgcagggaca tctgccgcat cacgacgaga cgatcgagac acgcgaggcg
     2581 gacgacaatg cgccgttctc ggcactcgcg ttcaagatca tgaccgaccc gttcgtcggt
     2641 aagctgacct atttccgggt gtactcgggc tcactgccgt cgggcagcta catctacaac
     2701 tcgacgaagg atcgccgtga gcgcatcggt cgtctgctgc agatgcatgc caaccggcgc
     2761 gaagagatcg aagaggttta cgcgggcgac atcgcagcgg ccatcggttt gaaggagacg
     2821 cgtacgggcg acacgctgtg cgatccgaac aagccgatca tcctggaagc gatgaagttc
     2881 ccggagccgg tgatcgaagt tgccatcgag ccgaagacga aagcggacca ggaaaagctg
     2941 ggcgccgcgc tgcagaag
//
LOCUS       DASURG010000213         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1468913, whole genome shotgun sequence.
ACCESSION   DASURG010000213 DASURG010000000
VERSION     DASURG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468913"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /gene="nuoG"
                     /locus_tag="VFY80_03605"
     CDS             complement(<1..1416)
                     /gene="nuoG"
                     /locus_tag="VFY80_03605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX6007021.1"
                     /translation="MVKIEIDGKEYETPEGSTIMDAANRAGIFVPHFCYHRKLSIAAN
                     CRMCLVQVEKAPKPLPACATPVTNGMKVWTHSDEAVDAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASDSRYAEEKRVVVNKNLGPLIATDMTRCIHCTRCVRFGTEIA
                     GIMELGMIGRGEHAEIITFVGKTVDSELSGNVIDLCPVGALTSKPFRYTARPWELVQR
                     SSVSPHCGLGANLTVQVKQNRVMRVLPRENEAINECWLSDKDRFSYEGLNSDERLARP
                     LLKCDGVWREVEWPVALDYIANGLKRIRDAHGARAIGALATPHQTLEELYLLQKLMRG
                     LGTGNVDFRLRQSDFSADRKLRGVPWLGMNVADIGKLDRVLVVGSTLRKDHPLLAHRL
                     RQAAKRGTQVNLLNPFDDELLMRVANRAIVAPHAMPDALAQVLVAVLRARNAVTPAEL
                     RNALAGVESSEAARRIAESLAS"
     gene            complement(1426..2739)
                     /gene="nuoF"
                     /locus_tag="VFY80_03610"
     CDS             complement(1426..2739)
                     /gene="nuoF"
                     /locus_tag="VFY80_03610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX6007022.1"
                     /translation="MNAPLPPFSPDVYGPDALILKDLTGLNWRLKDYEARGGYQALRK
                     ILEQKIAPVDIVNEVKKSALRGRGGAGFPTGLKWSFMPQQYQGAKYIVCNSDEGEPGT
                     FKDRDILRYNPHIVIEGMLIAGYATGSAAGYNYVHGEIWDVFERCEEALAEARAAGYV
                     GRNILGSDFSFELYNHHGYGAYICGEETALLESLEGKKGMPRFKPPFPASYGLYGKPT
                     TINNTETFAAVPWIILNGGEAYLNLGRPNNGGTKIFSVSGDVERPGNYEVRLGTPFAQ
                     LLEMAGGMRGGKKLKACIPGGSSMPVLPADIMMKTDMDYDSIAKAGSMLGSGAVIVMD
                     ETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRMVERIEHGNGKLEDLDLLNEVS
                     DNIQGRTICALGDAAAMPVRAFVKHFRDEFMHHIEHGKCLVPDYV"
     gene            complement(2736..3215)
                     /gene="nuoE"
                     /locus_tag="VFY80_03615"
     CDS             complement(2736..3215)
                     /gene="nuoE"
                     /locus_tag="VFY80_03615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX6007023.1"
                     /translation="MLSPEALLKIDQAVAKYPPERKQSAVMAALTIAQDEKGWLSVET
                     LDFVAQYLGMPPIAVYEVASFYTMYDLKPVGRHKLAICTNLPCALQGAAAAAQHLKRR
                     LGIEFGETTGDGCFTLKEAECMGACGDAPVMLHNNKTMLSWMTPEKLDALLNELKTK"
     gene            complement(3227..>4189)
                     /locus_tag="VFY80_03620"
     CDS             complement(3227..>4189)
                     /locus_tag="VFY80_03620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6007024.1"
                     /translation="IRVMFDEITRILAHTFGLGAHGLDLGAMSVFLYCFREREDLLDV
                     YEAVSGARMHAAYYRPGGVYRDLPERMPQYTSNIQGEAALRERNRNRQGSLLDFIEDF
                     TERFPRHVDEYETLLTDNRIWKQRTVGIGVVPPERALALGFTGPMLRGSGIEWDLRKK
                     QPYAVYDRLAFDVPVGVNGDCYDRYLVRIEEMRQSNRIIRQCVDWLRSNPGPVMADSH
                     KVTPPSRVAMKSNMEELIHHFKLFTEGMHVPEGEAYAAVEHPKGEFGVYLISDGANKP
                     YRMKVRSPSFTHLSSIDELARGHMIADVVAIISTYEFVFGEVDR"
BASE COUNT          703 a         1343 c         1338 g          805 t
ORIGIN      
        1 cgaggcgagg ctctccgcga tgcgccgtgc ggcttccgag gactcgacgc cggccagcgc
       61 attgcgcagc tcggccgggg tcaccgcatt tcttgcacga agtaccgcca cgagaacctg
      121 cgcgagagcg tcgggcatgg cgtgcggtgc aacgatcgcg cggttcgcga cgcgcatcag
      181 cagctcgtcg tcgaacggat tgagcaggtt cacctgcgtg ccgcgctttg ccgcctgacg
      241 caggcgatgc gcaagcagag gatgatcctt gcgcagcgtg ctgccgacga ccagcacgcg
      301 gtcgagcttc ccgatgtccg cgacgttcat gccgagccac ggtacaccgc gcagtttgcg
      361 gtccgcgctg aaatccgact ggcgcagcct gaaatcgacg ttgccggtgc cgagcccgcg
      421 catgagcttc tggagaagat acagctcttc gagcgtctgg tgcggtgtcg cgagcgcgcc
      481 gatcgctcgc gccccgtgcg catcgcggat gcgcttgagc ccgttcgcga tgtaatcgag
      541 cgcaacgggc cattccactt cccgccagac gccgtcgcac ttgagcagcg gccgcgctag
      601 gcgctcgtcg gagttcagtc cttcgtatga aaagcggtcc ttgtccgaaa gccagcattc
      661 gttgatcgct tcgttctcgc gggggagcac gcgcatgacg cggttctgct tcacctgtac
      721 cgtgaggttc gcgcccaaac cgcagtgcgg gctcaccgaa cttcgctgga cgagctccca
      781 gggccgtgcg gtatagcgga acggcttgga ggtcaacgca cccaccggac acagatcgat
      841 cacgttgccg gaaagctcgg aatcgacggt cttgccgacg aaggtgatga tttccgcatg
      901 ctcgccacgg ccgatcatgc cgagctccat gatgccggcg atctcagtgc cgaagcgcac
      961 acagcgcgtg cagtggatgc agcgggtcat gtcggtcgca atgagcggcc ccaagttctt
     1021 gttgaccacg acgcgctttt cctccgcgta gcgcgaatcg ctcgcgccgt agcccaccgc
     1081 gagatcctgg agctggcatt cgccgccctg atcgcagatc gggcaatcga gcgggtggtt
     1141 gatgaggagg aactccatca cgcccttctg ggcgtccacc gcctcgtcgg agtgcgtcca
     1201 gaccttcatg ccgttggtga ccggtgtcgc gcacgccggc agcggcttgg gcgccttttc
     1261 cacctgcacc aggcacatgc ggcagttggc tgcgatcgaa agcttgcggt ggtagcagaa
     1321 atgcggcacg aagatgccgg cgcggttcgc agcatccatg atggtgctgc cctcgggggt
     1381 ctcgtattcc ttgccgtcta tctcgatctt caccatcgcc cgtgctcaaa cgtaatccgg
     1441 cacgaggcac ttcccgtgct cgatatggtg catgaattcg tcgcgaaaat gcttgacgaa
     1501 ggctctgacc ggcatggccg cggcgtcgcc cagcgcacag atggtgcggc cctgaatgtt
     1561 gtccgagacc tcgttcagaa gatccaggtc ttcgagcttg ccgttgccgt gctcgatgcg
     1621 ctccaccatg cggtagagcc agccggtgcc ttcgcggcac ggcgtgcact ggccgcacga
     1681 ttcctcgtag tagaagtacg agagccgcag cagcgagcgc accatgcagc gcgtctcgtc
     1741 catcacgatg actgcgcccg agccgagcat ggaacccgct ttcgcgatgg agtcgtagtc
     1801 catgtcggtc ttcatcatga tgtcggcggg cagcacgggc atcgaggaac cgccggggat
     1861 gcaggctttc agctttttgc cgccgcgcat gccgcccgcc atttcgagga gctgcgcaaa
     1921 cggcgtgccg agccttactt cgtagttgcc cgggcgctcg acgtcaccgg ataccgagaa
     1981 aatcttggtg ccgccgttgt tcgggcgccc caagttgaga tacgcctcgc cgccgttcag
     2041 gatgatccac ggcacggctg cgaacgtctc ggtgttgttg atggtcgtgg gcttgccgta
     2101 gagcccgtaa ctcgcgggga acggcggttt gaagcgcggc atgcccttct tgccctcgag
     2161 cgactcgaga agcgcagttt cctcgccgca aatgtacgcc ccatagccat gatggttgta
     2221 gagctcgaag ctgaaatcgc tgccgagtat attgcgcccg acgtaacccg cggcgcgcgc
     2281 ttccgcgagc gcttcctcgc agcgctcgaa gacgtcccag atttcgccgt ggacgtagtt
     2341 gtacccggcg gcgctgccgg tcgcgtaacc cgcgattagc atgccttcaa tcacgatgtg
     2401 cgggttgtag cgcaggatat cgcggtcctt gaacgtgccc ggctcgccct cgtcggaatt
     2461 gcagacgata tatttggccc cctgatactg ctgcggcatg aagctccact tgagcccagt
     2521 ggggaacccg gcgccaccgc gcccgcgcag ggccgatttc ttcacctcgt tgacgatgtc
     2581 caccggcgca atcttctgct cgaggatttt cctgagcgcc tggtagccgc cgcgcgcttc
     2641 gtagtccttc aaccgccagt tgagtccggt caggtccttc agtatgagcg cgtccggtcc
     2701 gtagacgtcc ggagaaaagg gagggagcgg cgcgttcatt tggttttcag ctcgttgagg
     2761 agcgcatcga gcttctcggg cgtcatccac gagagcatcg tcttgttgtt gtggagcatg
     2821 accggcgcgt cgccgcacgc gcccatgcac tcggcttcct tcagtgtgaa gcagccgtcc
     2881 ccggtcgttt caccaaactc gatgccgagt cggcgcttca gatgctgtgc ggccgcggcg
     2941 gcgccctgca gcgcgcaggg cagattggtg cagatggcga gcttgtgtcg cccgaccggc
     3001 ttcaaatcgt acatcgtgta gaaggaggcg acctcgtaga ccgcaatcgg cggcatgccg
     3061 agatactgcg cgacgaaatc gagcgtctcg accgacagcc agcctttctc gtcctgcgcg
     3121 atcgtgagcg ccgccatcac cgccgactgc ttgcgctcgg gcggatactt ggcaaccgcc
     3181 tggtcgatct tgaggagcgc ctcgggcgag agcaccatcg gcgcgttcat cgatccacct
     3241 ccccgaacac gaattcgtag gtgctgatga tggccaccac gtcggcgatc atgtggccgc
     3301 gcgcgagttc gtcgatcgac gagaggtgcg tgaagctcgg cgagcgcact ttcatgcggt
     3361 acggcttgtt ggcgccgtcg gagatgaggt agacgccgaa ctcgcccttc gggtgctcga
     3421 cggctgcgta cgcctctcct tcggggacgt gcatgccctc ggtgaacagc ttgaagtgat
     3481 ggatcagctc ttccatgttg gacttcatcg ccacgcgcga gggcggcgtc accttgtggc
     3541 tgtccgccat caccggcccg gggttgctgc gcaaccagtc cacgcactgc cggatgatgc
     3601 ggttcgactg gcgcatctcc tcgatgcgaa cgaggtaacg gtcgtagcag tcgccgttga
     3661 cgccgaccgg aacgtcgaac gccagccggt cgtacaccgc gtagggctgc ttcttgcgca
     3721 ggtcccactc gatgcccgag ccgcgcagca tcggccccgt gaatcccagt gcgagcgcgc
     3781 gctcgggcgg cacgaccccg atgcccaccg tgcgctgctt ccagatgcgg ttgtcggtga
     3841 gtaacgtctc gtactcatcg acgtgacggg ggaagcgctc ggtgaagtct tcgatgaaat
     3901 cgagcagcga gccctggcga ttgcggttgc gctcgcgcag cgccgcttcg ccttggatgt
     3961 tcgacgtgta ctgcggcatg cgctcgggca gatcgcggta cacgccgccg ggccgatagt
     4021 aggccgcgtg catgcgcgcg ccggacaccg cctcgtacac atcgagcagg tcctcgcgct
     4081 ccctgaagca gtacaggaac accgacatcg cgccgagatc caggccgtgc gcgcccaaac
     4141 caaatgtgtg ggcgaggatg cgcgtgatct cgtcgaacat cacgcggat
//
LOCUS       DASURG010000214         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1161433, whole genome shotgun sequence.
ACCESSION   DASURG010000214 DASURG010000000
VERSION     DASURG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161433"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFY80_03625"
     CDS             complement(<1..853)
                     /locus_tag="VFY80_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6007025.1"
                     /translation="MKALVLRGPHQRFELTTVPDPVAGAGEAVARVIACGAGLTIQHI
                     KAGRIAARFPRIIGHEITGEIVEVGPGVQGLTVGDAVTAYYYLYCGQCRRCLANLEPL
                     CERSGGNVGKDCDGGYAEYIKLPERVFIRLPDALDARTHPAEIGVVTDALATPYKVLK
                     RAQVKAGETVAVFGAGGGLGIHQLVLAKWARARVIAIDLKRDKLEAGRKAGADELVDA
                     AAVDPVEALMDLTRGAGVDVVIDYVSTTATLEAAARSLGSRGRLVTLGGAGETFRASS
                     LQMLLKEQ"
     gene            complement(860..2044)
                     /locus_tag="VFY80_03630"
     CDS             complement(860..2044)
                     /locus_tag="VFY80_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6007026.1"
                     /translation="MEAMYSVNEEQAAILETVKRFVDEEVRPRAPKLDADPDPAAGFS
                     WEIVEKAHALGIRTMTLSEKWGGLGTDSLTTAMVIEELAKGDIGVSVIFAQTLKIAQI
                     MEKALNAEQQARVLPQFCADPRGMLAIGITEPDNASNYFLPYPVPFRTSARKAQGGWV
                     VNGMKHFISNGNRASFYLLFVQTDAGKPMAEGTTCFLVERGRAGFTIGRVHDKMGERL
                     ANNAELVFQDCFIPDENVVGEAGRGFGVLAEFFPQSNAYAAATILGVAAALYRKAVEW
                     AQVRVQGGKSLIQHDGIRAQLAEMRMLIDASRAYLHRAAWLADHQSQGWDRTLGALPK
                     AMASQCAWKIATWTMEIHGGHGYMKEFGVEKLVRDAAAFLHSDGVNRTLFLKAANFMF
                     HD"
     gene            complement(2063..3454)
                     /locus_tag="VFY80_03635"
     CDS             complement(2063..3454)
                     /locus_tag="VFY80_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6007027.1"
                     /translation="MVRLTETLARFIHDTGLADLPAGAVEKAKKVIADTFAVILAGAG
                     SEVAVPLLRYVECAGESGASPILGTSRTATPEVAALVNGTFGHALDFDDVLSMMPAHP
                     SAVILAALCAGLAGERVSGARLLEAYALGIEVGGKIGLGITVGHYHRGFHGTGTLGIF
                     SALAALAKLHRADPETTGIAFGIAASMASGLQRNFGTMTKPLHTGWAARSALAAVRLA
                     TCGFTAAPDVLEGKAGFFAAYGVGESDPQVTAAALGKPWVLLDPGIALKRFACCYASH
                     RGMDGVLELKRRHRFAAEDVERLSCRMPPGGMRVLTYPRPKTGLEGKFSLEYALAAGA
                     LDGRYTLWSFTDEAVQRPAIALLLERIAATEDARCRGDDPLFDKRSPGSRGFVEVELA
                     LKAGVRDTIRIDRPPGHPARELTWDDIRAKFADCASQARLPAVRAREAFEHIGALDRV
                     EDVSRILDLLRLN"
     gene            3623..4876
                     /gene="hemA"
                     /locus_tag="VFY80_03640"
     CDS             3623..4876
                     /gene="hemA"
                     /locus_tag="VFY80_03640"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX6007028.1"
                     /translation="MQLFAFGVNHQTAPLSVREQLVFHAEMLTGALRDLVDRQPVREA
                     AIISTCNRTEVYCATAEPQLATEWLASYHRMTPGQIEPYLYRLPQARAVKHAFRVASG
                     LDSMVLGEPQILGQFKQAVRTAQAAGTLGWLLSKLFQRTFSVAKAVRSETDIGTSTVS
                     MASAAVELAERIYPSISGQSVLFIGAGEMIELVAAHFAARHPRRVTFANRTPERAEQL
                     AQRFLGRTISLNELPGQLAVHDIVVASTASPLPLIGKGLVESALKARKHRPMLMFDLA
                     VPRDIEGEVAKLDDVFLYTVDDLGRIAREGMDVRRNAVTQAEVIIENQVSDFMHWLEN
                     RELVPAIRALRDTAERARRHEIERALRRLARGDDPRLVMEYLSHALTNKLMHGPTHAL
                     SHAAAGEREALSELLGRLYQIHRPQ"
     gene            4911..5999
                     /gene="prfA"
                     /locus_tag="VFY80_03645"
     CDS             4911..5999
                     /gene="prfA"
                     /locus_tag="VFY80_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX6007029.1"
                     /translation="MKESIITKLAQLSRRLEEINQLMSSESATADMGNYRKLAREHAE
                     IAPVVKLYEAYRSGEDDIRTAQEMSGDPAMREFAESEIRETRERLGRIEVELQKQLLP
                     RDPNDERNIFLEIRAGTGGDESALFAGDLIRMYTRFAERNGWQVEVISQSPSDLGGYK
                     EIIAKIVGRGAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIADVVLN
                     PAELRIDTFRASGAGGQHVNKTDSAIRVTHLPTGLVVECQDDRSQHKNKARALAVLAA
                     RLKDKQIREQQMKTAATRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYRIAQIMD
                     GDLFELTDALIAEHQAEQLAQLGEEATA"
BASE COUNT         1086 a         2011 c         1975 g         1054 t
ORIGIN      
        1 cctgctcctt caacagcatc tgaagggagg aggcgcggaa cgtctcgccc gcgcccccga
       61 gggtcacaag ccgcccgcgg ctccccaggc ttcgggcggc ggcttcgagc gtcgccgtgg
      121 tcgagacgta atcgatgacg acatcgactc ccgcaccgcg cgtcaaatcc atgagggctt
      181 cgaccggatc gaccgccgcg gcgtcgacca gctcgtccgc gccggcctta cgacccgctt
      241 caagcttgtc gcgcttgagg tcgatcgcaa tcacccgggc acgtgcccac ttcgcgagca
      301 cgagctgatg gatgccgagt ccgccccccg caccgaacac ggcgacagtt tctccggctt
      361 tgacctgcgc gcgcttcagc accttgtacg gcgttgcgag cgcgtcggtc accacgccga
      421 tctcggccgg atgcgtgcga gcgtccagcg cgtccggcag cctgatgaat acccgctccg
      481 gtagcttgat gtattccgcg tagccgccgt cgcagtcctt gccgacgttg ccgccgcttc
      541 tttcgcacag cggctcgaga ttggcgaggc agcgtcgaca ctgcccgcag tagagatagt
      601 agtaggccgt caccgcgtct ccgacggtaa ggccttgcac gccgggaccg acttccacga
      661 tttcgccggt gatctcgtgc ccgatgatgc gcgggaagcg ggcggcgatg cggccggcct
      721 tgatgtgctg aatcgtgagt ccggcgccgc acgcgatgac gcgcgccacc gcttcgccgg
      781 cccctgcgac cgggtccgga accgtcgtga gctcgaagcg ctggtgcggg ccgcgcagga
      841 cgagcgcctt catggcactt cagtcgtgaa acatgaaatt ggcggccttg aggaagagcg
      901 ttcgattcac gccgtcggag tgcaggaagg ccgccgcgtc gcgcacgagc ttctccacgc
      961 cgaactcctt catgtagcca tggccgccgt ggatttccat ggtccacgtc gcgatcttcc
     1021 acgcgcactg cgaggccatc gctttcggga gcgcaccgag cgtccggtcc cagccttgcg
     1081 actggtgatc ggcgagccac gccgcgcgat ggaggtaagc acgcgaggcg tcgatcagca
     1141 tgcgcatctc ggccaactgc gcgcggatgc cgtcgtgctg gatgagcgac ttgccgccct
     1201 gcacacggac ctgcgcccac tcgaccgcct tgcggtagag cgctgctgcg acaccgagga
     1261 tcgtcgcggc ggcataagcg ttgctctgcg gaaagaactc ggccagcacg ccgaaacctc
     1321 tgcccgcctc gccgacgacg ttttcgtccg ggatgaagca atcctggaag acgagctcgg
     1381 cgttgttcgc gagacgctcg cccatcttgt cgtgcacgcg accgatggtg aagccggccc
     1441 gcccgcgctc gacgagaaaa catgtcgtgc cttccgccat cggttttccg gcatcggtct
     1501 gaacgaagag cagatagaag ctcgcgcggt tgccgttgga gatgaagtgc ttcatgccgt
     1561 tcacgaccca gccgccctgc gccttgcggg cgctggtgcg aaatggaacg ggatacggca
     1621 ggaagtagtt cgatgcgtta tcgggctcgg tgatgccgat cgcgagcatg ccgcgcggat
     1681 cggcgcagaa ctgtgggagc acgcgcgcct gctgttcggc attcagtgcc ttttccatga
     1741 tctgggcgat cttgagggtc tgcgcgaaga tgactgaaac gccgatgtcg cccttcgcga
     1801 gttcttcgat caccatggcg gtcgtcagcg aatccgtgcc gagcccgccc catttctcgg
     1861 agagcgtcat ggtgcggatg ccgagcgcat gcgccttctc tacgatttcc caggagaatc
     1921 ctgcagcggg atccggatcg gcatcgagct tcggcgcacg cgggcgcact tcctcatcga
     1981 cgaaccgctt caccgtttcc agtatggcgg cctgttcttc gtttacgctg tacatcgctt
     2041 ccatgttgcc ctcagatcga tttcagttca ggcgcaggag atcgagaatc cggctcacgt
     2101 cttccacgcg gtcgagcgcg ccgatgtgct cgaacgcttc ccgcgcacgt accgccggaa
     2161 gccgggcttg acttgcgcag tcggcaaact ttgcgcgaat gtcgtcccag gtgagttcgc
     2221 gcgccgggtg tccgggcgga cggtcgatgc gtatggtatc gcgcacgccg gccttgagcg
     2281 cgagctcgac ctcgacgaaa ccgcggctcc cgggactgcg cttgtcgaag agcggatcat
     2341 cgccccggca ccgggcgtcc tccgtcgcgg cgatccgttc gagcaggagg gcgatggcgg
     2401 ggcgctgcac cgcttcgtcg gtaaacgacc agagcgtgta ccggccgtcg agcgcaccgg
     2461 cggcgagcgc gtactccagg ctgaattttc cttccagccc ggttttcgga cgcgggtagg
     2521 tcaagacgcg catgccgccc ggcggcatgc ggcatgagag gcgctcgaca tcctcggctg
     2581 caaagcggtg acgccgtttc aattcgagca cgccgtccat gcccctgtgc gaggcgtagc
     2641 agcaggcaaa gcgcttgagt gcgatccccg ggtcgagcag cacccagggc ttcccgagag
     2701 ctgcggcggt gacctgcgga tccgattcgc cgacgccgta cgccgcaaag aagcccgcct
     2761 tgccctccag cacgtcgggt gctgcggtga atccacaggt tgcgagccgt actgcagcga
     2821 gcgcgctgcg cgccgcccac cccgtatgca acggtttcgt catcgtgccg aagttgcgct
     2881 gcaatccgct tgccatggaa gcggcgatgc cgaacgcaat gcctgtcgtt tcgggatcgg
     2941 ctcgatgcaa cttcgcgagc gcggcgagtg cggaaaagat gccgagcgtg ccggttccat
     3001 gaaatccgcg gtgataatgg ccaacggtaa tgccgagtcc gattttcccg cccacttcga
     3061 tgcccagagc gtaggcctcc agcaaacgcg cgccgcttac cctttcgccg gcgaggcccg
     3121 cgcacagtgc ggcgaggatc acggcgctgg gatgcgcggg catcatggag agcacgtcgt
     3181 cgaaatcgag cgcatgaccg aacgtgccgt tcaccagcgc cgcgacctcg ggcgttgccg
     3241 tgcgcgaggt gccgaggatc gggcttgcgc cactctcgcc ggcgcactcg acatagcgca
     3301 ggagcggcac ggccacttcg cttcctgccc ctgccagaat gacggcaaag gtatcggcga
     3361 tgaccttctt cgctttctcg actgcgccgg ccgggagatc ggcaaggccc gtgtcatgaa
     3421 tgaagcgggc gagcgtttca gtgagtcgga ccatgctaga gtgacaggga tgggcgcgat
     3481 cggtcagcgt ggccggttcg tgctgctgtc ccactatagc agagaacaga aatggaaccg
     3541 cttcgcgggg cgcccttcgt agcacgacgt gactggagca tataaaatat agcccctgtt
     3601 ccgggcctgc aatatcaagg acatgcagct cttcgccttc ggcgtcaatc atcagaccgc
     3661 gcccctttcc gtgcgcgagc aactggtttt tcacgccgaa atgctgaccg gggcactgcg
     3721 tgatctcgtc gaccggcagc cggtgcggga ggctgcgatc atatcgacct gcaaccgcac
     3781 ggaagtgtat tgcgcgacgg cggagccgca gctcgcgacc gagtggcttg cgagctatca
     3841 ccgcatgacg cccggtcaga tcgaacccta cctctacagg ctgccgcagg cgcgcgcggt
     3901 aaagcacgcg tttcgagtgg caagcggtct tgattccatg gtgctcggcg agccgcagat
     3961 cctcggtcaa ttcaagcagg ccgtgcgcac cgcccaggcc gccggcacgc tcgggtggct
     4021 gctgagcaaa ctttttcaga ggaccttctc agttgccaag gcggtgcgca gcgaaaccga
     4081 catcggcacg agcacggttt cgatggcttc ggccgcggtc gagctcgcgg aacgcatcta
     4141 tccgagcatc tccgggcaaa gcgtgctctt catcggcgca ggcgaaatga tcgagctggt
     4201 cgcggcacac ttcgctgcac gtcatccccg ccgggtgacc tttgccaacc ggacgccgga
     4261 acgcgccgag caactcgcgc agcgtttcct cggtcgaacg atctccttga atgagcttcc
     4321 cggacagctt gccgtgcacg atatcgtcgt tgcgagtacc gcgagcccgc tgccgctgat
     4381 tggcaagggg ttggtggaaa gcgcactcaa ggcgcgcaag caccggccaa tgctcatgtt
     4441 cgatctcgcc gtgccgcgcg acatcgaggg cgaggtggcg aagctcgacg acgtcttcct
     4501 ctataccgtg gacgacctgg gacggatcgc acgcgaaggc atggatgtcc gtcgcaatgc
     4561 ggtgacgcag gccgaagtga tcatcgagaa ccaggtgtcg gatttcatgc actggctcga
     4621 gaatcgcgag ctcgtcccgg ccatccgcgc attgcgcgac acggccgaac gggcgcgacg
     4681 gcacgagatc gagcgcgcat tgcggcgcct tgcccgcggc gacgatccac ggcttgtgat
     4741 ggagtacctc tcacacgcgc ttacgaacaa gctcatgcac ggccccactc atgccctgag
     4801 tcatgccgcc gcgggcgagc gggaggcgct ttccgagctg ctcgggcgcc tctatcagat
     4861 tcaccggccg cagtgaccgc gcggccctca cttccgaaag ccggccccca atgaaggaga
     4921 gcatcatcac caagctcgcc cagctctcgc gccggctgga ggagatcaat cagctcatgt
     4981 catcggagag cgccactgcc gacatgggca attatcgcaa gcttgcgcgc gagcacgccg
     5041 agatcgcacc ggtggtcaag ctctacgagg cgtatcgcag cggcgaggac gatatccgca
     5101 ccgcgcagga aatgtcgggc gatccagcga tgcgcgaatt cgcggagtcg gagatccgcg
     5161 agacgcgcga gcgcctgggc aggatcgagg tcgagcttca gaagcagctg ctgcccaggg
     5221 accccaacga cgagcgcaac atcttcctcg agattcgcgc cggcaccggg ggcgacgagt
     5281 cggcgctctt cgcgggcgat ctcatccgca tgtacacacg gtttgccgag cgcaacggct
     5341 ggcaggtcga agtgatctcg cagagtccgt ccgacctcgg cggctacaag gagatcattg
     5401 ccaagatcgt cggccgcggc gcgtattcca agctcaagtt cgagtcgggg ggacatcgcg
     5461 tgcaacgcgt gcccgcgacc gagacgcaag gtcgcattca cacttcggcg tgtaccgtcg
     5521 ccgtcatgcc ggaagcggac gaaattgcgg atgtcgtgct caacccggcg gagctgcgca
     5581 tcgatacttt ccgtgcgtcc ggcgccggcg gacagcacgt caacaagacc gattccgcga
     5641 tccgcgttac gcacctgccg acgggactcg tggtcgagtg ccaggacgat cgctcgcagc
     5701 acaagaacaa ggcgcgcgcg ctggcggtgc tcgccgcgcg cttgaaggac aagcagatcc
     5761 gcgagcagca gatgaagacc gcggcgaccc gcaagtcgct gatcggcagc ggcgaccgct
     5821 cggagcgcat ccgcacctac aattttccgc aggggcgtgt caccgaccac cgcatcaatc
     5881 tcacgctcta caggatcgcg cagatcatgg acggcgacct cttcgagctg accgacgcgc
     5941 tgatcgccga gcatcaggcc gagcagctcg cgcagctcgg cgaggaagcg acggcctagc
     6001 gttacccggg atgacgacga tcggtggggc cctcgctgcg gtcggcagcc gcatcgatgc
     6061 acgcgaggcg cgcgtgctgc tcgcccatgt tctggagaaa gacgcgacct atctagccgc
     6121 ctaccc
//
LOCUS       DASURG010000215         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_38241, whole genome shotgun sequence.
ACCESSION   DASURG010000215 DASURG010000000
VERSION     DASURG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38241"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFY80_03650"
     CDS             complement(<1..672)
                     /locus_tag="VFY80_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6007030.1"
                     /translation="MSQLSLAIDIGGTFTDIVVYDPQAARHYIYKEYTTPDDPARGVV
                     TGVRALLERDRIPASSIKRVVHATTLFTNALIERKGVKCGLITTEGFRDILEIQTERK
                     YELYDIFIEMPRPLVPRLLCREVPERMSELGVVETPLDLDALHREVDFLIDHGVESIA
                     VMFLHSYVNPAHEQAVLEALARRHPRLHLTASFEVAPEIREYERLSTTVVNAYIKPLA
                     QRYLDR"
     gene            complement(676..1647)
                     /locus_tag="VFY80_03655"
     CDS             complement(676..1647)
                     /locus_tag="VFY80_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007031.1"
                     /translation="MKDVLRYALLAVAVSAAPATLAQSYPVKGKPIRIIVPYAPGGVA
                     DLLSRIVGQRLGPLYGTQIVIDNRPGAGGHVGGELTAKSAPDGYTIMLGTTTHNAAYA
                     MYSKLAYSPPKDLRPIVLLAESQGVLLVHPTVPVKTVKEFLALARGRPGELNYASAGP
                     GSATHLMAELLKMVGGIELTHIAYKGSGPAMIDLMSGHVETMVENVSTAAPHIKARKV
                     RALGVTSRKRDQNLPDVPTIAESGLPAYEAVPYYTMSTAAKTPNEIVRKLSVDVDAIV
                     RHPELATRWNELGITPLGGSPEAAEKRNVVETEKWTKVIKTAAIRAD"
     gene            1738..1989
                     /locus_tag="VFY80_03660"
     CDS             1738..1989
                     /locus_tag="VFY80_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007032.1"
                     /translation="MRAAATLRAFGPNSESVRGRKEIRARHVSARYLDMSNPLKREAA
                     REQTRCGLHEHHARVGRIEAAELLRHAEARELGNRAKTV"
BASE COUNT          367 a          680 c          730 g          385 t
ORIGIN      
        1 gcggtcgaga tagcgctgcg cgagcggctt gatgtacgcg ttcaccacgg tcgtcgacag
       61 ccgttcgtat tcgcggatct cgggcgcgac ctcgaacgag gccgtgagat gcagccgcgg
      121 gtgccgccgc gccagcgcct ccaataccgc ctgctcgtgg gccggattga cgtacgagtg
      181 caggaacatc acggcgatcg attcgacgcc gtggtcgatc aggaagtcga cttcgcggtg
      241 cagggcatcg agatcgagcg gtgtttccac cacgcccagc tcgctcatgc gctcgggcac
      301 ttcgcggcac aacagccgcg gaacgagcgg gcgcggcatc tcgatgaaga tgtcgtagag
      361 ctcgtacttg cgctcggtct gtatctcgag aatgtcgcga aagccttcgg tcgtgatgag
      421 cccgcacttc acccccttgc gctcgatcag cgcgttggtg aagagcgtgg tcgcatgcac
      481 gacccgcttg atggacgagg cagggatgcg gtcgcgctcg agcagcgcgc gcacgccggt
      541 gacgaccccg cgcgccgggt cgtcgggcgt ggtgtactcc ttgtagatgt agtgcctggc
      601 agcctgcggg tcgtagacga cgatgtcagt gaacgtgccg ccgatatcga tcgcgagcga
      661 aagctgggac atcgatcagt cggcccggat tgcggcggtc ttgatgacct tggtccactt
      721 ctcggtctcg acgacgtttc gcttctctgc ggcttcgggc gatccgccga gcggcgtgat
      781 gccgagctcg ttccagcgcg tggcgagctc aggatggcga acgatcgcat cgacgtcgac
      841 gctgagcttg cgcacgatct cgttcggcgt cttcgccgcg gtggacatcg tgtagtaggg
      901 caccgcctcg taagcgggca ggcccgattc cgcgatggtg ggcacgtcgg gcaggttctg
      961 gtcgcgcttg cggctggtga cgccgagggc gcgcaccttg cgggccttga tgtgaggtgc
     1021 ggcggtcgac acgttctcga ccatcgtttc gacgtggccc gacatgagat cgatcatcgc
     1081 ggggccgctg cccttgtacg cgatgtgcgt caattcaatc ccgccgacca ttttcagcag
     1141 ctccgccatg agatgggtcg cagaccccgg gcccgcggag gcgtagttga gctcgcccgg
     1201 gcgcccacga gcgagcgcga gaaactcctt caccgtcttc acgggcacgg tcggatgcac
     1261 cagcagcacg ccctgcgatt cggcgagcaa tacgatgggt ctcaggtctt tcggcggact
     1321 gtaggcaagc ttggaataca tagcatacgc cgcgttgtgc gtcgtcgtcc ccagcatgat
     1381 cgtgtagcca tcgggcgccg acttcgcggt cagctcgccg ccgacgtggc cgcccgcgcc
     1441 ggggcggttg tcgatcacga tctgagtgcc gtacagcggt ccgagccgct ggcccacgat
     1501 gcggctcaag aggtctgcga ccccgcccgg ggcgtacggc acgatgatgc gtatcggctt
     1561 gcccttgact ggatacgact gcgcgagcgt agccggcgcg gctgagactg cgactgcgag
     1621 cagcgcatag cgcaagacgt ctttcacgtg ccctcccgtt gacgtgtgaa cagcaatgtt
     1681 cttcaccgct tcttgcatcc gtcccgactg tatcgccggc ccggtcaacg gtcaatcgtg
     1741 cgtgcggcgg caacgttgcg tgcgttcggc ccgaacagcg agtccgtgcg cggcaggaaa
     1801 gaaatacggg cccgccacgt cagcgcgcgc tacctggata tgtcgaaccc gctcaagcgg
     1861 gaagccgccc gggagcagac gcggtgcggc ctgcacgagc accacgcgcg cgtcggccgg
     1921 atcgaagcgg cggaactcct tcgccatgcc gaagcgcgcg agctcggcaa tcgcgccaag
     1981 accgtgtaag tattgggcgc aaggaacgac agcggatcct gcagtcccaa cccacctgtc
     2041 atagaaagga gagtcgcatg aaatcgcaag cgttgagaat cgcaaccgct gtgtccatga
     2101 cggtcgcgct cgccggaggg cttggcgccg gaaccgccac tgcggcggag atgatggaaa
     2161 ac
//
LOCUS       DASURG010000216         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_752624, whole genome shotgun sequence.
ACCESSION   DASURG010000216 DASURG010000000
VERSION     DASURG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752624"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..393
                     /locus_tag="VFY80_03665"
     CDS             91..393
                     /locus_tag="VFY80_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX6007033.1"
                     /translation="MILEVAILHIKPESMKEFEADFPKAEAIVSSIPGYISHELQRCV
                     ETKGRYHFLIRWETIDAHLVNFRQSPKFQDFRAIVGPHFAQPPVAEHFERVTANRL"
     gene            complement(450..884)
                     /locus_tag="VFY80_03670"
     CDS             complement(450..884)
                     /locus_tag="VFY80_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6007034.1"
                     /translation="MKKAKSGSKRNYDERFKRGMEVLRKMGRESLMMNQQELYPDMYE
                     MSVGHLFGDVWGRPGLSLRDRQLVTLAANIALCRPSGNHSHYLSAMHLGITKEEICEV
                     MIQVGHYAGWPTLSNAVRQFTAIMEEQEQAKKLGKRFRSKLL"
     gene            990..1973
                     /locus_tag="VFY80_03675"
     CDS             990..1973
                     /locus_tag="VFY80_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007035.1"
                     /translation="MASRVERMFAMWIAAITALIVPAASAAEYPTKPIRLVVPSAPGG
                     GTDIIARLIAQGLGDAWRQTVVVDNRGGAGGVAGVTIVAKQSAPDGYTMLLGSVGHLA
                     FVPAVRTNLGYDPLKDLTPISLAAVQPFLVAASLSLPAGSIKELVAQAKSKPDTIKYG
                     SGGSGSASHLGIELLQLTAGVKMLHVPYKGSNPAITALMGNEIQIALAGLATVLPHAR
                     AGRLKALAVTGAKRAQIAPEVPTVAESGVPGYRFDVWYGLVFPGGTPRAIVEKTHAEV
                     VRLLKHPDVSKRFATAGVEPQTNTPDAFRELIAREAATWSKVAKSANIRVE"
     gene            2071..>3007
                     /locus_tag="VFY80_03680"
     CDS             2071..>3007
                     /locus_tag="VFY80_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6007036.1"
                     /translation="MSATATRVSQPRDHKLIVEKDVKIPMRDGTMLYADVFRPDTAEK
                     VPVILNISVYQKDKLWVPPADLEEKANPYMNWETVNPLWWCPRDYACVRVDSRGSGKS
                     PGKSEPSSHQEALDSYDAIEWIAKRPWCSGNVGMLGISYHAASQWRAANLQPPALKAI
                     MPWEGRADQYRDQAYHGGIFAMGFIGNWWLTHTAHQLLGRPRSYNPDAFHNDMMWLYM
                     RNDLDSEYWRQCSAQWDKINVPVYSVGNWGGFSMHLRGNTEGFVCAASKHKKLRIHTG
                     THFHPFHSEEGRMDQLRWFDHWLKGIDTGIMDEPPV"
BASE COUNT          585 a          934 c          931 g          557 t
ORIGIN      
        1 ccggcgaaag ccggaatcca gaactgcatg gctggatgcc ggttttcacc ggcatgacga
       61 atctgttgcg taactcgata aggagaagcc atgattctcg aagtcgcaat tctgcacatc
      121 aaacccgaat cgatgaagga attcgaagcg gacttcccga aggcagaagc aatcgtgagc
      181 agcatccccg gctacatttc acacgagctg cagcggtgcg tggaaacgaa gggccgttat
      241 cacttcctga tccgttggga gacgattgat gcgcacctgg tcaatttccg ccagtcgccc
      301 aagtttcagg acttccgcgc catcgtcggc ccgcacttcg cgcagccgcc ggtggcggag
      361 cacttcgagc gcgtcaccgc caaccgcctc tgagcagctc gcggggctac ccgagcttgg
      421 cgctcccctg attcagggga ccttcccggt tataggagtt tggagcggaa gcgcttgccg
      481 agcttcttcg cctgctcctg ctcttccatg atggcggtga actggcgcac ggcgttggag
      541 agcgtcggcc agcccgcgta atgcccgacc tgaatcatca cttcgcagat ctcttccttg
      601 gtgatgccaa ggtgcatggc gctgagataa tgcgagtggt tgcccgacgg acggcagagc
      661 gcgatgttgg ccgccagcgt gacgagctgg cggtcgcgca ggctcaggcc gggtcgtccc
      721 cacacgtcgc cgaagagatg gccgacagac atctcgtaca tgtccgggta aagttcctgc
      781 tggttcatca tgaggctctc gcgccccatc ttgcgcagca cttccatgcc gcgcttgaag
      841 cgttcgtcgt agttgcgttt cgatccggat ttcgccttct tcatggtgtg cagtcccgat
      901 tgagcaaatt gaggattttt ttcgcgacgc tttttatgtg atcatgtcat ccacgcacgc
      961 atccggcaag cgttgcagga ggaggaacga tggcatcgcg cgttgaacga atgttcgcaa
     1021 tgtggattgc ggcgatcacg gccctgatcg tgccggccgc gagcgcggcg gagtatccga
     1081 ccaagccgat tcgcctcgtg gttccgagcg cgccgggtgg cggaaccgat atcatcgcgc
     1141 gcctgatcgc acaggggctc ggtgacgcct ggaggcaaac cgtcgtagtg gacaaccgcg
     1201 gcggcgcagg cggcgtcgcc ggtgtcacga tcgttgcgaa gcaatctgcg ccggacggtt
     1261 acacgatgct cttgggaagc gtagggcacc tcgccttcgt gcccgcggtg cgcacgaact
     1321 tgggttacga tccgctgaag gacctcacgc cgatttcgct cgctgcagtt cagccgttcc
     1381 tcgttgccgc gtccctctcg ctcccggcgg gatcgataaa ggagctggtc gcccaggcga
     1441 aaagcaagcc cgataccatc aagtatggct ccgggggcag cggctctgcc tcgcacctcg
     1501 gcatcgagct gctgcagctc accgccggcg tcaagatgct gcacgttccg tacaagggca
     1561 gcaatccagc gattactgcg ctcatgggta acgaaatcca gatcgcgctc gccggactgg
     1621 ccacggttct gccccacgcc cgtgcagggc ggctgaaggc gctcgccgtg acgggcgcga
     1681 agcgtgcgca gatcgcaccc gaggtgccga cggtcgcgga atccggcgtg ccgggctacc
     1741 ggttcgacgt gtggtacgga ctcgtcttcc cgggcgggac gccgcgtgcc atcgtcgaga
     1801 agacccacgc cgaggtggtg agactcctga aacaccccga tgtcagcaag cgattcgcga
     1861 cggccggcgt ggagcctcag accaataccc ccgacgcgtt tcgcgagctg attgcccgtg
     1921 aggcagcgac atggtcgaag gtcgcgaaga gcgcgaatat ccgcgtcgag tgaagctctg
     1981 gacagtcagt agcccggcgc cgtaaagtgc cgacctgcag ttttcgtgtg cgacgtctct
     2041 aagaccgttc ccatccggag ggtaaattca atgagtgcca ctgccacgcg cgtgtcgcaa
     2101 ccgcgcgacc acaaactgat cgtcgagaag gatgtaaaga tcccgatgcg cgacggcacg
     2161 atgctctacg ccgacgtgtt ccgcccggat accgccgaga aggtgcccgt catcctcaac
     2221 atcagcgtct accagaaaga caagctgtgg gtgccgcccg ccgatctgga agaaaaggcg
     2281 aacccctaca tgaactggga gacggtaaac ccgctgtggt ggtgcccgcg cgactacgcc
     2341 tgcgtgcggg tcgattcgcg cggctccggg aaatcgccgg gcaagtcgga accgagctcg
     2401 caccaggaag cgctcgacag ctacgatgcg atcgagtgga tcgcgaaacg gccgtggtgc
     2461 tcgggcaacg tgggcatgct cggcatttcg tatcacgccg cctcacaatg gcgggcggcc
     2521 aatctgcagc cgcctgcgct caaggccatc atgccttggg aaggccgcgc cgaccagtat
     2581 cgcgatcagg cctaccacgg cggcatcttc gccatgggat tcatcgggaa ttggtggctc
     2641 acgcacaccg cgcaccagct tctgggacgc ccccgcagtt acaacccaga cgccttccac
     2701 aacgacatga tgtggctcta catgcgtaac gatctcgatt ccgagtattg gcgccagtgc
     2761 agcgcccaat gggacaagat caacgtgccg gtctacagcg tcggtaactg gggcgggttc
     2821 tccatgcatc tccgcggcaa tacggaaggc ttcgtgtgcg cggcatccaa gcacaagaaa
     2881 ctgcgcatcc acaccggcac ccacttccat ccattccatt ccgaagaggg gcggatggat
     2941 cagttgcgct ggttcgatca ctggctgaaa ggcatagata ccggcatcat ggatgagccc
     3001 ccggtga
//
LOCUS       DASURG010000217         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1162087, whole genome shotgun sequence.
ACCESSION   DASURG010000217 DASURG010000000
VERSION     DASURG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162087"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VFY80_03685"
     CDS             complement(<1..860)
                     /locus_tag="VFY80_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEX6007037.1"
                     /translation="MTTRRHFLRASGAMVGATLVSKAAQGSVPEAPIQTKPETQPPLV
                     PSNGRPYNPVVTLNGWSLPWRMNNGWKEFHLVAEPVRRQLAPGMTAHLWGYNGQSPGP
                     TIEVVEGDKVRIFVTNKLPEHTTIHWHGVILPNGMDGVGGLTQPHIPVGKTFVYEFVP
                     KRSGTHMYHPHADEMVQMAMGMMGLLIIHPRDPNVMRVDRDFAFLMAAYDIEPGSYTP
                     KVATMLDFNLFTWNSRVFPGIDALPVRLGDRVRIRMGNLTMTNHPIHLHGHDVEVAGT
                     DGGWVPKAAR"
     gene            complement(872..2299)
                     /locus_tag="VFY80_03690"
     CDS             complement(872..2299)
                     /locus_tag="VFY80_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX6007038.1"
                     /translation="MKRKHPKGLRTAAAIVSVALLGGCATLSQDAGFGAVQSAATERG
                     LKQDAKWLRDERDREAAREAVKKLLEKPLSPDAAVQVALLNNRGLQATYADVGVAEAD
                     VVQAGRLRNPGFSYARLRRGDEIEIERTFLFDVLGLITLPIRTELEKRRYELTRNRVA
                     AEILQVAADTRRTWYRAVGAQEAAKYAEQVKEAAEASAELARRLAAAGNFSKLDHARE
                     QVFYAEATAQLARARQAAFSERERLNRLMGLWGEDIAYTLPDRVPELPKSARDIRDVE
                     TRAMDKRLDVKGAMTEADNIAASLGLTRATGFISVLELGYQRNSETHEPRQTGYEIEV
                     RLPIFDWGGARNARAEYTYMQAVNRAADTAVKARSEVREAYHAYRTAYDLAKHYRDEI
                     VPLRKRISDENVLRYNGMLISVFELLADARAQIAAVNAYIDALCAFWIAEASLELAIN
                     AGSPGATTLQAGSMRAGAGAAEAGH"
     gene            complement(2310..2678)
                     /locus_tag="VFY80_03695"
     CDS             complement(2310..2678)
                     /locus_tag="VFY80_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007039.1"
                     /translation="MRTSFAFLLYGAWLIALPAHAQSRPSDPVQRDARVLEMKYESAF
                     RSYRLYREEDLGSWREVNDEVGRLGGHVGMFRSAGHGAKEQEPMKPQAGAPASRAQDM
                     PGQASSRGEPTAPQSHRMTR"
BASE COUNT          480 a          947 c          949 g          566 t
ORIGIN      
        1 cagcgtgctg ctttgggcac ccagccgccg tccgtgcccg cgacttccac gtcgtgcccg
       61 tgcaggtgga tggggtggtt ggtcatggtg agattgccca tgcggatgcg aacgcgatca
      121 ccgagacgca cgggcagcgc gtcgattccg ggaaagaccc ggctgttcca ggtgaagaga
      181 ttgaagtcca gcatggttgc aaccttcggc gtatagctgc cgggctcgat gtcgtaggcc
      241 gccatcagga acgcgaagtc gcgatcgacc cgcatgacat ttgggtcgcg cgggtggatg
      301 atgagcagcc ccatcatgcc catcgccatc tgcaccatct cgtcggcgtg cgggtggtac
      361 atgtgcgtgc cagagcgctt cggcacgaac tcgtagacaa aagtcttccc gaccggaatg
      421 tgcggctgcg tgagtccgcc cacgccgtcc atgccgttcg gcaggatgac gccgtgccaa
      481 tggatcgtgg tgtgctcggg caacttgttc gtgacgaaga tgcgcacctt gtcgccttcg
      541 acgacttcga tggtgggtcc cggcgactgg ccgttgtagc cccagaggtg cgcggtcata
      601 cccggagcga gttgccggcg cacgggctcg gcgacgagat ggaattcctt ccagccgttg
      661 ttcatgcgcc acggcagcga ccagccgttc aaggtcacta ccgggttgta aggccgcccg
      721 ttggagggca cgagcggcgg ttgcgtctcg ggtttcgtct ggatcggtgc ttcgggcacc
      781 gagccctgcg cagctttgct caccagggta gccccgacca tggcccccga tgcgcgcaag
      841 aaatgacgac gtgtagtcat gagtagtcct ctcaatgtcc cgcttcagcg gcaccggctc
      901 cagcgcgcat gctgcccgcc tgcagcgtcg tcgcacctgg agaaccggcg tttattgcca
      961 gctccaggct cgcctccgcg atccagaagg cgcaaagcgc atcgatatag gcgttgacgg
     1021 cggcgatctg cgcgcgcgca tccgccagca gctcgaatac gctgatcagc atgccgttgt
     1081 agcgcagcac gttctcgtcc gagatgcgct ttctgagcgg cacgatctca tcgcgatagt
     1141 gcttcgcgag gtcgtatgcg gttcgatacg cgtggtacgc ctcgcgcact tccgatcgcg
     1201 ccttcaccgc agtatcggcg gcgcggttga ccgcctgcat gtaggtgtat tcggcccggg
     1261 cattgcgcgc gccgccccag tcgaagatcg gaaggcgcac ctcgatctca tatccggtct
     1321 ggcgcggttc atgtgtttcg ctgttgcgct ggtagcccag ttccagcacg ctgatgaacc
     1381 cggtcgcacg cgtgagcccc agagaagcgg ctatgttgtc ggcttccgtc atcgcgcctt
     1441 tcacatcgag ccgcttgtcc atcgcccgcg tctcgacatc gcgaatgtcg cgcgcggact
     1501 tcggaagttc cggcacgcgg tcgggcaagg tgtacgcgat atcttctccc catagcccca
     1561 tcaggcggtt gagccgctcg cgctcggaga acgcggcctg gcgtgcccgg gcgagctgtg
     1621 ccgtcgcttc cgcataaaac acctgctcgc gcgcgtgatc cagcttgctg aagttacccg
     1681 cagccgccag ccgccgcgcg agttccgcgc tcgcttcggc ggcttccttc acctgctcgg
     1741 cgtacttggc ggcttcctgc gcgccgaccg cgcggtacca cgtgcggcgg gtgtccgccg
     1801 cgacctgcag aatctcggcg gcgacgcgat tgcgggtgag ttcgtaacgg cgcttttcca
     1861 gctccgtccg aatggggagc gtaatgaggc ccagcacgtc gaacagaaaa gtgcgctcga
     1921 tctcgatttc gtcgccgcgt cgcagccgcg cataggagaa ccccggattg cgcagccgac
     1981 cggcctgcac gacgtcagct tcagcgacgc cgacatcagc ataggtcgct tggaggccgc
     2041 ggttgttgag gagcgcgacc tgtacggccg catccgggga aagcggcttc tccaagagct
     2101 tcttcaccgc ttcgcgggct gcctcacggt cccgctcgtc gcgcagccat ttggcgtcct
     2161 gcttcagccc acgctccgta gcggcagatt gcacggcgcc gaagccggca tcctgggaaa
     2221 gggttgcgca gccgcccaga agcgcgacgc tcacgatggc cgcggccgtg cgcaagcctt
     2281 ttgggtgttt tcgtttcacg tgggcctctt cagcgggtca tgcgatgcga ctgtggcgcc
     2341 gtgggttcgc cgcgcgagga tgcctgtccg ggcatgtcct gcgccctgga tgccggcgcg
     2401 ccagcctgcg gcttcatcgg ctcctgttcc tttgcaccat gcccggcact ccgaaacata
     2461 ccgacgtggc ccccgagacg cccgacttcg tcgttcactt cgcgccacga cccaaggtct
     2521 tcttcgcggt acagtctgta gctgcggaat gccgactcgt atttcatctc gagcacccgc
     2581 gcatcgcgct gcaccgggtc cgacggcctg gactgcgcgt gagccggcag ggcaatcagc
     2641 cacgcgccgt acagcagaaa tgcaaatgaa gttcgcatcg ttctcctcgt tcttcatccg
     2701 tgcgttcggc gaacgcacaa cagatcctcc cggcttaatc agggaaggac aggatcgcta
     2761 gaacgggatc aggtgcgggg agggcgctgc ggccgttcgg gaacgaacag cgtatggagc
     2821 agggagagcg aggattcgag ggcgggcagt tgatccggga cgtttaccac ggcgatggtc
     2881 ggcatgcccg aaaaaacgtg atggcaattg agatgtccgg aatgatccat ggcagcgtcg
     2941 cc
//
LOCUS       DASURG010000218         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_855481, whole genome shotgun sequence.
ACCESSION   DASURG010000218 DASURG010000000
VERSION     DASURG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855481"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFY80_03700"
     CDS             complement(<1..587)
                     /locus_tag="VFY80_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6007040.1"
                     /translation="MKRRSFIAAAGAVTGAAGMLHAPAVIAQQKYRWRMPTTWTPALN
                     VMLGSAQRLAKMVAELSGGRLTIDVFPAGQIVSPLGVFEAASKGTVEAFMGASYYWNA
                     KDPAVQWFCSVPFGMNPSGMSSWYFHGGGLKLWEDVYAAFNLVPRPGPATGPQMAGWF
                     RKKINTIADYKGLKMRIPGLGGQVIAKAGGTVVLTP"
     gene            complement(615..1865)
                     /locus_tag="VFY80_03705"
     CDS             complement(615..1865)
                     /locus_tag="VFY80_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6007041.1"
                     /translation="MLIVRTNLRPLERAVGRASAFLFLFSLAHVGLCSEAPIRIGASV
                     SVTGQFAELGQTQQRGQELCVRQVNARGGLLGRKVEIVTQDDRSQASDVVAIYEKLLG
                     QDKVDLVFSPYSSPLTEAVANVTEKYRMPMIAAGAATTSIFKKGRKYIFMLLSPAEIY
                     LEGLIDVAAKRGLKTIAILYEDTLFPKAIALGTVELAKKRGIKAVVLEAYPRQTTDFA
                     PLLAKLKAANPDVVAAATYFNDAVAITRQMKAAGVNPKMHAVTVGGDVPKFYETLGRD
                     AEFVYGASQWLPELVTLRAGGLIPIARQYPGAREFTEDYGKAFPGAGLSYHTAQGYAA
                     CQILLDAVKRAGSLDRDRIRNAILGFEGNTAYGAFKVDADGFQIAHKMVMFQWQDGKK
                     VIVWPEELAADRPRFPTPPWTQRR"
     gene            complement(1967..>2829)
                     /locus_tag="VFY80_03710"
     CDS             complement(1967..>2829)
                     /locus_tag="VFY80_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6007042.1"
                     /translation="VLATAMLLQAAWRQRSWRSYALAGTELLWFAGQVQIMGVLVGWI
                     SAELFRLDPACALPLYLVLQYLFVERFVRAREQAAREQQEAIIAERARILQDMHDGMG
                     SQLVTALRLVRREDGDRAIAARTIEEALQDLRLIIDSLDEANQGLLPKLADLRYRLEP
                     RLAELGIRLDWDVQPLPELDVLPPQSALNVMRIVQEALNNAVRHARPVTITVSTARQD
                     GAVIRVADDGSGFDPDTAKGAGRGLAGMRKRAEQIGATLRVERQAAGGTSISLHFPPA
                     TALHPRIATA"
BASE COUNT          502 a          936 c          876 g          515 t
ORIGIN      
        1 ggggtcagca cgactgtccc gcccgccttg gcgatgacct ggccgccgag gccgggaatg
       61 cgcatcttca gacccttata gtctgcgatg gtattgatct tcttcctgaa ccagccggcc
      121 atttgcgggc ccgtggccgg acccggccgc ggaacgaggt tgaacgcggc gtaaacgtcc
      181 tcccacaact tgagtccccc gccgtggaag taccagctcg acatgccgct cggattcatg
      241 ccgaagggca cgctgcagaa ccactgcacg gcgggatcct tcgcgttcca gtaataggac
      301 gcccccatga atgcctccac cgtgcccttg gacgccgctt cgaacacgcc gagcggcgag
      361 acgatctgcc cggccggaaa tacgtcgatc gtgagccgcc cgccactcag ttcggccacc
      421 atcttcgcga gccgctgtgc cgagccgagc atcacgttga gggccggggt ccacgtcgtc
      481 ggcatgcgcc agcgatattt ctgctgcgcg atgacggcgg gtgcgtggag catgccggcc
      541 gcaccggtaa ctgcgcccgc cgcggcgatg aagctgcgtc gtttcattct ctcccctttg
      601 tcgttcagca gcgcctaacg ccgctgggtc catggcggcg tcgggaaccg cggccggtca
      661 gccgcgagtt cttccggcca cacgatcact ttcttgccgt cctgccactg gaacatcacc
      721 atcttgtgcg cgatctggaa accgtccgcg tccaccttga acgcgccgta agccgtattg
      781 ccctcgaagc ccagaatcgc attgcgtatc ctgtcacggt cgagggagcc tgcgcgcttc
      841 accgcgtcga gcagaatctg acacgctgcg tagccttgag ccgtgtggta ggaaagcccc
      901 gcccccggga atgccttgcc gtagtcttcg gtgaattcgc gcgcgccggg atactgccgc
      961 gcgatgggaa tgagaccgcc cgcacgcagc gtgacgagct cggggagcca ctggctcgcg
     1021 ccgtatacga actccgcgtc ccgtccgagc gtttcgtaaa acttcggcac gtcgccgccc
     1081 accgtgaccg cgtgcatctt cggattcacg ccggcggctt tcatctgccg ggtaatcgcg
     1141 acggcatcgt tgaagtaggt tgcagccgcg acgacatccg gattggccgc cttcaacttc
     1201 gcgagcagcg gtgcgaaatc ggtcgtctgg cgcggatacg cttcgagcac gaccgccttg
     1261 atcccgcgct ttttcgccag ctctaccgtg ccgagcgcga tcgccttggg aaaaagcgta
     1321 tcctcgtaga gaatggcgat cgtcttcagc ccacgtttgg cagcgacatc gatgagacct
     1381 tctagataaa tctccgcggg cgacagcagc atgaaaatgt acttccgtcc cttcttgaag
     1441 atggaagtag tcgccgcacc tgcggcgatc atcggcatgc ggtatttttc ggtgacgttc
     1501 gcgacggctt cggtgagcgg cgaagaatag ggcgaaaaaa cgagatcgac tttgtcctgc
     1561 ccgagcagct tctcgtagat cgcaacgacg tccgacgcct gcgagcggtc atcctgcgtg
     1621 acgatctcaa cctttcggcc gagcaggccg ccccgcgcgt tgacctgcct cacacacaat
     1681 tcctggcccc gctgttgtgt ctgaccgagt tcggcaaact gcccggttac ggagacggag
     1741 gcaccgatgc gaataggcgc ctcggagcag agcccgacgt gcgccaatga gaaaagaaag
     1801 aggaacgcgc tcgcgcgccc gacggccctc tcaaggggcc gtaaattggt gcggacaatc
     1861 aacatacgtc tccccccgtc gtgtgacgcc cgacgagccg catcgtagca cggaaggaga
     1921 gccgcacatc ccgtgttttt ggtatttttg tcggaggtgc cgcccgctat gccgtagcaa
     1981 tacgcggatg gagagcggtg gcgggcggaa aatgcagcga tatggaggtg cctcctgcgg
     2041 cttgtcgctc gacgcgcagg gtcgcgccga tctgctccgc tcgcttccgc atgccggcga
     2101 gtccgcggcc cgcccctttg gccgtgtccg gatcgaatcc gctaccgtcg tccgcgacac
     2161 ggatgactgc accatcctgc cgtgcggtcg ataccgtgat cgtcacgggt cgagcatgcc
     2221 tgaccgcgtt gttcagcgcc tcctggacga tgcgcatcac gttgagtgcc gactgcggtg
     2281 gcaggacgtc gagctcgggc agcggttgaa catcccaatc gagccgtatg ccgagttcag
     2341 ccaggcgtgg ctcgaggcgg tagcgcaggt ccgcaagctt gggtagtagg ccctgattcg
     2401 cctcatcgag agaatcgatg atgagacgca ggtcctgcaa cgcctcctct atcgtccgcg
     2461 cggcgatcgc gcggtcgccg tcctcgcgcc tcaccaggcg cagcgcggtg acgagctgcg
     2521 agcccatgcc gtcgtgcatg tcctgcagga tgcgtgcgcg ctccgcgatg atcgcctcct
     2581 gctgctcgcg cgccgcctgc tcccgcgcgc gcacgaagcg ctcgacaaac aggtattgca
     2641 gcaccaggta cagcggcaag gcgcatgctg gatccaagcg gaacagctcc gcggaaatcc
     2701 atccgacgag aacgcccatg atttgaacct gaccggcgaa ccagaggagc tcggtgcccg
     2761 ccagcgcata gctgcgccag gagcgctgac gccaggccgc ctgcaggagc atggcggtcg
     2821 caagcaccc
//
LOCUS       DASURG010000219         7428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_39100, whole genome shotgun sequence.
ACCESSION   DASURG010000219 DASURG010000000
VERSION     DASURG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39100"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="VFY80_03715"
     CDS             complement(<1..1884)
                     /locus_tag="VFY80_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX6007043.1"
                     /translation="MNDTAILEGSAATSAARADAQSVTTLSEHVVEIVSDSGEGAQRC
                     GQSFGAIAARSGNGVWTVEIIPAEIQPPARSIAGASGNRVRIGSCRVTNGGDEADLVV
                     AFNEQVLIGRVRAGELKPGCLILLESMWREDRDPKIAHSYVSTVDKLKAEGYKVHEIP
                     MERECKPLVDDPRRGKNMFVLGMLCSLYNLDMELAREQVAFTFRKKDAGVIRTNHMLL
                     EAGYKWARAHLGFSFRIPAERAREAQIVVNGNTALALGVLASGMEVCAMYPITPATSA
                     SHYLSEVFEKVGGIVHQAEDEIAACSFAIGASYAGKCAVTITSGPGYSLKQEAIGLAV
                     MGEIPLVVVNVQRGGPSTGQPTKAEQGDLLTACFGSHGDAPKVVLAASSIEDCFYSII
                     TARKIAETFNMVVVVLSDANLATSQQPFARPQFSEAWLAPPFDQSPVPDGVQPYDWDA
                     ATGLARRFVPGQPNGMHCLTGLAHDRASHVAYDPQVNEEAIRARSLKLAALQKTLKLA
                     PVFGDEGGDLLIIGWGSTQGAIEEAVEHVRRDGLKVSSLHLRFLQPLPSGIKEVMQRF
                     RRVMTVEGNWCDRADDEIIDENNRRYSALAMLLRARYLVDVDCWSEVKGQPMKPGTLV
                     RVIR"
     gene            complement(1881..3407)
                     /gene="gltA"
                     /locus_tag="VFY80_03720"
     CDS             complement(1881..3407)
                     /gene="gltA"
                     /locus_tag="VFY80_03720"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX6007044.1"
                     /translation="MAAKKKTVRTIPQQRAPVREQDPHIRRENFAEVTCSFRVEDALH
                     EAERCLLCPDPACVEGCPVSIDIPGFIRRISAQDFRGAYDVITATNLLPAICGRVCPQ
                     ESQCEGVCPVGETLEPVAIGRLERWVGDHAIAEHWSSIPHIERNGFRIGIVGSGPAGM
                     ACAADMAKAGCAVTVYEAFHQPGGVLRYGIPDFRLPNAVIDAEIANLRKLGVHFECNT
                     LVGRLFTIEQMIEEMGYDAVFIGTGAGYPSLLGIPGDSLNGVLSANELLTRCNLMVAR
                     EFPRYDTPIPIGRAVAVIGSGNTAMDAMRVCLRLGADKVHCIYRRTKAESPARAEEIH
                     HAEEEGIEFHWLTHPVEILDDGKGNVRGMRCVRMELGEPDDSGRRRPVQVAGSEFEFA
                     CDMVVFAIGTNANPIIGQTSRLKLSKRGYIETDADLATSMAGVYAGGDIVTGAATVIE
                     AMGAGRRAAQSMKAYLGIRDSSIVYQSEPRGGGKRFGIDLSERNFVRVRLPDREEVVA
                     "
     gene            complement(3455..4441)
                     /locus_tag="VFY80_03725"
     CDS             complement(3455..4441)
                     /locus_tag="VFY80_03725"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX6007045.1"
                     /translation="MTATGGLSFEVVTREDFSDVTFLLEIHHPLMAKAARPGQFVIVM
                     AHEHGERIPLTIADFDRQRGTVTLVVQAVGKTTREMQQTCVPGAALYGIAGPMGVPSH
                     TTDARKIVCVGGGLGVAPIFPQMRAFKEAGAYVIAVVGFRSRELVFWEDKFRACCDEY
                     IVCTDDGSAGIKGLVTDGIKLALEKHPDIDEVVAIGPPIMMKACAEATRARKVRTVVS
                     LNPIMVDGTGMCGGCRVKVGGQVKFACVDGPDFDGHQVDFDDLMKRLRRFAQEEKAAL
                     ERWSASCRMKAESAAVALPAAMAAAAPVVGPELTSSDKGLELPDPFEEVEGG"
     gene            4686..5102
                     /locus_tag="VFY80_03730"
     CDS             4686..5102
                     /locus_tag="VFY80_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6007046.1"
                     /translation="MRVRDFMSTPAVTIRSDQDYKTGLRLMQQNAVHHLPVVDADGRV
                     VGIAAERDLLLAATRYLDSSVEVGEVMHRGAVTTRPDAPLATAASLMLEHHIGSLPVI
                     DAEAHVIGLVTETDLFRLLVGADEDEGPGTIPPPVV"
     gene            complement(5120..6103)
                     /locus_tag="VFY80_03735"
     CDS             complement(5120..6103)
                     /locus_tag="VFY80_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007047.1"
                     /translation="MKSVRRSRIAIALVCCGFQVATTAAQAQEKYPARPIRILIPFPA
                     AGAADTIGRTVGEQLSLQLGQPVVIDNRPGAAGRLATEMLARAEADGHTLLVGGVGPL
                     SISPGLYRKLPYEAERDFAPVTRAAEIINVMVVNAATGVATPRQFIDWARKRQAVRFG
                     SSGPGQFDHLVGEFFQREAKLSMTHVPYKGGGPALVDMVTGDLQVMFATYVTAVPHIR
                     GGRLRAIAVSTPERQPILSDLPAVSETIAGFGASNWNGIFAPARTPRAIITRLHREIQ
                     RAMQHPEVKKRQNAVGIEPGGSASPAEFANFIREDTRRWARIIQAAGIRLE"
     gene            complement(6133..7164)
                     /locus_tag="VFY80_03740"
     CDS             complement(6133..7164)
                     /locus_tag="VFY80_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6007048.1"
                     /translation="MKAAVLHGAGDLRHETVADPVPASGEVIVKVEACSICGSDLHGF
                     HGKHPRLTFPRILGHEFAGEVVALGRDVAQVSTGTRVCCDIDLYCGQCGPCREGRTNI
                     CEKLRTMGFDRDGAYAEFVAVPAINLYPLPENVSYDQAAAVQVLGISYHAVAHRVRPR
                     PGEQVAVIGAGPIGLGAALIAKSLRAEVTVFEVLDYRLAMARKLACDRVLNPTHIDVR
                     GEALAATGGRGFDKVMECVGGFQEKTVRMAVDMVKRGGQITIVGTFPENRATIPIAYI
                     KDREIDINFSRGNFEAFAPCLDLIAQGRIDPDLYISHRLPLARAVDALRLLEARDVEA
                     HKIVLHPQA"
BASE COUNT         1287 a         2462 c         2322 g         1357 t
ORIGIN      
        1 gcggatcacg cggacgagcg ttccgggctt catgggctgg cccttcactt cgctccagca
       61 atcgacgtcc acgaggtaac gcgcgcgcag cagcatggcg agcgccgaat agcggcgatt
      121 gttttcgtcg atgatctcgt cgtctgcgcg atcgcaccag ttgccctcga ccgtcatcac
      181 gcgccggaag cgctgcatca cctctttgat gcccgagggc agaggctgca ggaagcgcag
      241 atgcagggac gacactttca gcccgtcccg gcgcacatgc tccaccgctt cctcgattgc
      301 accctgcgtg ctgccccagc cgatgatgag gagatcgcca ccctcgtcgc cgaaaaccgg
      361 cgccagcttg agcgtcttct ggagcgcggc cagcttgaga ctgcgcgccc gtatcgcctc
      421 ttcgttcacc tgcgggtcgt aggcgacatg gctcgcgcga tcgtgcgcga gtcccgtgag
      481 gcagtgcatg ccgttcggct ggccgggcac aaaccgccgt gcgagtcccg tcgccgcgtc
      541 ccagtcgtag ggctgcacgc cgtccgggac cgggctttga tcgaacggcg gcgcaagcca
      601 tgcctcgctg aactggggcc gcgcaaacgg ctgttgcgat gtcgcgaggt tcgcgtccga
      661 cagcacgacg accaccatgt tgaaggtctc ggcgatcttg cgcgcggtga taatcgagta
      721 gaagcaatct tcgatggagc ttgcggcgag gacgactttc ggcgcatcgc cgtgactccc
      781 gaaacaggcg gtcagcagat cgccctgctc ggccttggtg ggctggccgg tgctcgggcc
      841 gccgcgctgc acgttcacga cgaccagcgg aatttctccc atgacggcta ggccgatcgc
      901 ttcctgcttc agcgagtaac ccgggccgga ggtgatggta acggcgcact tgcccgcgta
      961 cgaggcaccg atcgcgaacg agcacgccgc gatttcgtct tccgcctgat ggacgatgcc
     1021 cccgactttc tcgaacacct cgctcagata gtgcgatgcg gacgtcgcag gcgtgatggg
     1081 gtacatcgcg cacacttcca tgccggaagc gagcacgccg agcgcgagcg ccgtattgcc
     1141 gttcaccacg atctgcgctt cccgcgcacg ttcggccgga atgcggaagc tgaagcccag
     1201 atgggctcgc gcccacttgt agcccgcttc gagcagcatg tgattggtgc gaatgacgcc
     1261 cgcgtccttc ttgcggaaag tgaaggcgac ctgttcgcgc gcgagctcca tgtcgaggtt
     1321 gtagaggctg cacagcattc cgagcacgaa catgttcttg ccgcggcgcg gatcgtccac
     1381 gagcggcttg cactcgcgct ccatcgggat ctcgtgcacc ttgtagcctt cggccttaag
     1441 cttgtcgacg gtcgagacat acgagtgtgc gatcttcggg tcgcggtctt cccgccacat
     1501 gctctcgagc aggatcagac atccgggctt caattcgcct gctcgcaccc ggccgatcaa
     1561 cacctgctca ttgaaggcga cgacgagatc cgcttcatcg ccgccgttgg tgacgcggca
     1621 ggatccgatt cgcacgcggt tgccgctcgc accggcgata ctgcgcgccg gaggctgaat
     1681 ctccgcaggg atgatctcga ccgtccacac gccgttgccg ctgcgtgccg cgatcgcgcc
     1741 gaaggactgc ccgcagcgct gcgcgccttc cccggagtcg ctcacgatct cgaccacgtg
     1801 ctcggagagt gtcgtgaccg actgggcgtc agcccgagca gcgctcgtcg cggcagagcc
     1861 ctccagaatt gccgtatcgt tcatgcgacg acctcctcgc ggtcggggag ccggacgcgc
     1921 acgaagttgc gttccgaaag atcgatgccg aagcgcttgc caccgccccg gggctcggac
     1981 tgatagacga tgctgctgtc gcgtatgccg aggtaggcct tcatgctttg cgccgcacgc
     2041 cggccggcac ccatcgcttc gatcaccgtc gccgcgccgg tcacgatgtc accgccggcg
     2101 tagacgcctg ccatcgaggt ggcgagatcg gcgtcggtct cgatatagcc gcgcttgctg
     2161 agcttgagcc gcgaggtttg tccgataatg gggttcgcgt tggtgccgat cgcgaagacc
     2221 accatgtcgc aggcaaactc gaactcgctg cctgcgacct gaaccggtct gcggcggccg
     2281 gagtcgtccg gttcgcccaa ctccatgcgc acgcagcgca tgccacggac gttgcccttg
     2341 ccgtcatcga ggatctcgac cggatgggtg agccagtgga attcgatacc ctcctcttcg
     2401 gcgtgatgga tttcctccgc acgcgccggg ctttcggcct tcgtgcggcg gtaaatgcag
     2461 tgcaccttgt cggcacccag gcgcaggcac acgcgcatgg catccatcgc agtgttgccg
     2521 gagccgatga ccgcaacggc gcggccgatc ggaatgggtg tgtcgtagcg cgggaattcg
     2581 cgtgcaacca tgagattgca gcgcgtgaga agctcgttcg cggagagcac gccgttcagc
     2641 gaatcgcccg gaatgccgag tagcgaggga taacctgcgc cggtgccgat aaagacagcg
     2701 tcatagccca tctcctcgat catctgctcg atggtgaaga gccggccgac cagcgtattg
     2761 cattcgaaat ggacgccgag cttgcgcagg ttcgcgatct cggcgtcgat cacggcgttg
     2821 ggcaggcgga agtccggaat gccatagcgc agcacgccac ccggctggtg aaacgcctcg
     2881 tacaccgtca ccgcgcatcc ggccttggcc atgtccgccg cgcacgccat gccggccggg
     2941 cccgagccga cgatcccgat gcgaaaccca ttgcgctcga tgtgcggaat gctcgaccag
     3001 tgctccgcga tggcatgatc cccgacccag cgctcgagcc ggccgattgc caccggctcc
     3061 agcgtctcgc ccaccggaca tacgccttcg cactggcttt cctgcgggca cacgcggccg
     3121 cagatcgcgg gcaaaagatt cgtcgcggtg atgacgtcat aggcgccgcg aaagtcctgc
     3181 gcgctgattc tgcggatgaa gcccgggatg tcgatgctga ccgggcagcc ttccacgcac
     3241 gccggatccg ggcacagcag gcagcgctcg gcctcgtgca gcgcgtcctc gacacgaaag
     3301 ctgcatgtca cttcggcgaa gttctctctg cgaatgtgcg ggtcctgctc acgcaccggc
     3361 gcgcgctgct ggggaatggt tcgtacggtc ttcttctttg ccgccatcaa tgcctccgga
     3421 gacccattgc gaaataagcg cgagtgcgca ttgtttagcc gccttccact tcttcgaacg
     3481 gatcgggcag ttccaacccc ttgtcggaag acgtgagctc tgggcccacg accggcgcag
     3541 cggccgccat ggcggcggga agcgcaaccg cggcgctttc cgctttcatc ctgcaacttg
     3601 cgctccagcg ctcgagcgct gccttttcct cctgcgcaaa gcgcctgagc cgtttcatga
     3661 gatcgtcgaa atccacctga tgaccgtcga aatccgggcc gtccacgcac gcgaacttca
     3721 cctgcccgcc caccttgacg cgacaccctc cgcacatccc ggtgccgtct accatgatcg
     3781 gattgaggct taccaccgtc ctcaccttgc gcgcccgcgt ggcctccgcg cacgctttca
     3841 tcatgatcgg cgggccgatc gcgacgacct catcgatgtc gggatgcttc tcgagggcga
     3901 gcttgatgcc gtcggtgacg agacccttga tgcccgccga tccgtcgtcc gtgcagacga
     3961 tgtactcgtc gcagcaagca cggaacttgt cttcccagaa tacgagttca cggctcctga
     4021 atccgacaac ggcgatcacg taggcgccgg cctccttgaa cgcgcgcatc tgcggaaaga
     4081 tgggcgccac accgagcccg ccgccgacgc atacgatctt gcgcgcatcg gtggtatggc
     4141 tcggaacccc catgggtccc gcgatgccgt aaagggcggc gcccggcacg cacgtctgct
     4201 gcatctcgcg cgtcgtcttg ccgaccgcct gcacgacgag cgtcacggtg ccccgctgac
     4261 ggtcgaaatc cgcgatggtg agcggtatgc gctcgccatg ctcgtgcgcc atcacgatca
     4321 caaactgacc ggggcgcgcg gctttcgcca taaggggatg atggatttcg aggaggaagg
     4381 tgacgtccga gaaatcctcc cgcgtcacca cttcgaagct caagccgcca gtcgccgtca
     4441 tgtcttaacg cacctgtctt gttgcggagc gtgttgcgaa tcgatctcga cttctggccg
     4501 tcaatgtaga ggcttcccct ggtgtcgata ttgatctagc gcaagatctc tgcgcacctc
     4561 agcgcctcgg acaccgcgca cacgggccgg attgccggag aaaagttgat gcatgtcagt
     4621 accgcggccg gcgtggggcg gacaatcatc agagcggcga atattggccc gcgaaggagt
     4681 tcaccatgcg cgtacgcgat ttcatgagca cgcccgccgt taccatccgc agcgatcagg
     4741 actacaagac ggggctacgc ctcatgcaac agaacgccgt gcatcacttg ccggtcgtgg
     4801 atgcggacgg gcgggtcgtc ggcatcgccg ccgaacgcga tctgctgctc gcagccacac
     4861 gatatctgga ttcttcggtc gaagtagggg aagtgatgca ccgcggtgcc gtgaccacgc
     4921 gcccggatgc gccgctcgcg acggcggcaa gcctcatgct ggagcaccac atcggaagtc
     4981 tgccggtaat tgatgccgaa gcgcacgtca tcggcctcgt gaccgagacg gatcttttca
     5041 ggctgctcgt gggcgcggac gaggacgaag gtcctggaac gattccgccg ccggtcgttt
     5101 agaagtacgg cgcgcggcgt tactcgaggc ggataccggc agcctgaatg atgcgcgccc
     5161 agcgccgggt atcttcgcgg atgaagtttg cgaactcggc gggagatgcg ctgcccccgg
     5221 gctcgatgcc tactgcgttc tggcgctttt tcacctccgg atgctgcatc gctctctgaa
     5281 tttcgcggtg gagccgcgtg atgatggcgc gcggcgtgcg cgcgggcgca aagatgccgt
     5341 tccagttgct cgcgccgaac cccgctatcg tttcgctcac cgcaggcaga tcggaaagga
     5401 taggctgccg ttcaggcgtg ctgacggcga tcgcgcgcaa ccgacctccg cgaatgtgcg
     5461 gcacggcggt gacataagtc gcgaacatca cctgtagatc gcccgtcacc atgtccacga
     5521 gcgccgggcc gccgcccttg tacggcacat gcgtcatgga gagcttcgct tcgcgctgaa
     5581 agaattcccc gacgaggtgg tcgaactggc cgggccccga gcttccgaag cgcactgcct
     5641 gccgctttct cgcccaatcg atgaattgcc gcggcgtcgc cacgcctgtt gcggcattga
     5701 cgaccatgac gttgatgatc tccgccgcac gcgttaccgg ggcgaagtcg cgttcggcct
     5761 cgtacggcag cttcctgtac agccccggac tgatcgagag cggtcccacg ccgcccacga
     5821 gcagcgtatg gccgtcggct tcggcacgcg ccagcatttc ggtcgcaagc cggccggccg
     5881 cgccggggcg gttgtctatc acgactggct gacccaattg gagtgagagc tgctcgccga
     5941 cggtgcgccc gatggtatcg gcagcgcctg ccgctggaaa cgggatgagg atgcggatcg
     6001 gccgcgcggg atatttctcc tgcgcttgcg cggctgtcgt cgcgacctgg aacccgcaac
     6061 acacgagggc gatcgcgatg cgcgaccggc gaacgctttt catgtctctc ctccagtgat
     6121 caaacaattc tcttacgcct gcgggtgcag gacgatcttg tgcgcttcca cgtcccgtgc
     6181 ttccagcagt cgcagcgcat cgaccgcacg cgcgagcggc aggcggtgac tgatatagag
     6241 atcgggatcg atcctgccct gcgcgatcaa atccagacac ggcgcgaacg cctcgaagtt
     6301 gccgcgcgag aagttgatgt cgatctcacg atccttgata tacgcgatgg ggatcgtggc
     6361 gcggttctcg ggaaaggtgc cgacgatcgt gatctgtccg ccgcgcttca ccatgtccac
     6421 cgccatccga accgtctttt cctggaaccc acccacgcac tccatgacct tgtcgaagcc
     6481 ccgcccgccc gttgccgcca gcgcttcgcc gcgcacatcg atgtgcgtcg gattgagcac
     6541 gcgatcgcaa gcgagcttgc gcgccatcgc gagccggtag tcgagcactt cgaagaccgt
     6601 cacttctgca cgcagggatt tggcaatcag cgctgcgccg agtccgatcg ggccggcgcc
     6661 gatgaccgca acctgctcgc cggggcgcgg ccgcacgcgg tgggccaccg cgtgatagct
     6721 gatgccgagc acctgcaccg ccgcagcctg gtcgtagctc acattttcgg gcaggggata
     6781 gagattgatc gcgggcaccg caacgaactc tgcgtaggcg ccatcgcgat caaaacccat
     6841 ggtccggagc ttctcacaga tgttggtgcg gccttcgcga cacgggccgc actgcccgca
     6901 ataaaggtcg atatcgcagc agacccgcgt tccggtcgac acttgggcga catcgcgccc
     6961 gagcgcgacc acctcgccgg caaattcgtg accgagaatg cgtggaaatg tcagccgcgg
     7021 atgcttgcca tggaagccgt gcagatccga accgcagatc gaacacgctt ccaccttgac
     7081 gatgacttct ccggaagcgg gaaccgggtc cgcgaccgtt tcgtggcgca ggtcgcctgc
     7141 gccatgtaga accgctgcct tcatggccga gctggataag tgatatcttc ggaacgttcc
     7201 gggcgccgga acgccgctat catagcccaa ctgttggagc gcaccgcgca acggtccggc
     7261 ggaaatagaa agccgcgcag gctctcaagc cttgcgcggc aaatacaaca cccacaggag
     7321 gagtgaactt tggtgttgcg cctgcatgat cgcgctgcgc gagggcttcg tctgtgacgt
     7381 gccgcacgct gcggcggcca ttccccgccg tttcgtgcga tagtgcac
//
LOCUS       DASURG010000220         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_244142, whole genome shotgun sequence.
ACCESSION   DASURG010000220 DASURG010000000
VERSION     DASURG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244142"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFY80_03745"
     CDS             complement(<1..516)
                     /locus_tag="VFY80_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007049.1"
                     /translation="MKIVDIPPYQRAGVNYSPTEGHFMMRDLIDSMKARGQLEGIEID
                     IDEGEPTDHGAETRDEVVVANIAVGVVKRVQALCALNKYDAIITQAGIEPGFLAAMSV
                     STLPVAYPVHSALHFASVLGEKFCALTTTDAQAMIVRRNAQLYGMNHKLTSVRYVSRS
                     STFTMALVRKYK"
     gene            675..1970
                     /locus_tag="VFY80_03750"
     CDS             675..1970
                     /locus_tag="VFY80_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6007050.1"
                     /translation="MEPCFLTAAAASNLIREGKLTSEALVRSCLERIAARDGDVRAWS
                     YVDPAQAIEQARELDKRAPLGPLHGIPFGVKDVIDTADMPTTQNSPHYQSHHPAKDAG
                     CVAVVRHSGALILGKTDTVEFAAGGRKALTRNPHNLAHTPGGSSSGSGAAVADFMVPL
                     AFGTQTGGSQIRPSSFNGVYALKPTWGAVSREGAKLCSAYCDTVGWYGRSVADLALVA
                     EAFRLRDLGAQQPVAVNGLRAAVCRTPFWNKAEPGAQKALSAAAERLEAAGVRVRALE
                     LPAKFAGMNEAQRTLMHGEGGAAFLPELLAFGERLHAEFRDMATNKRGITARMMVEAY
                     DLAADCARTFDAMFDEFDFVVAPAAPGEAPEGLSTTGDWVFNAMFTAMHVPCLAIPCT
                     RGEKNLPVGVQIVGPRFSDAQLLAIAAAVAPVVDAEKRP"
     gene            2051..>2255
                     /locus_tag="VFY80_03755"
     CDS             2051..>2255
                     /locus_tag="VFY80_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007051.1"
                     /translation="MASWRSVLPALALAIALHSTATPAADSAVTQYPTRPIRLIVGFP
                     PGSTDDYNARVLAPKLTERLGQQV"
BASE COUNT          375 a          739 c          748 g          393 t
ORIGIN      
        1 cttgtacttg cgcaccagcg ccatggtgaa ggtcgacgag cgcgagacgt agcgcacgct
       61 cgtgagcttg tggttcatgc cgtagagctg ggcgttgcgc ctcacgatca tggcctgcgc
      121 gtcggtggtc gtgagcgcgc agaacttctc gcccagcacc gacgcgaagt gcagcgccga
      181 gtgcacgggg tacgctacgg gaagcgtaga gaccgacatc gcggcaagga agcctggctc
      241 tatgcctgcc tgcgtgatga tggcgtcgta cttgttcagc gcgcacagcg cctgcacgcg
      301 cttcaccaca cccaccgcga tattcgccac gacgacttcg tcccgggttt ccgcaccgtg
      361 atcggtgggc tcgccctcgt cgatgtcgat ctcgatgccc tcgagctgcc cgcgcgcctt
      421 catgctgtcg atcaggtcgc gcatcatgaa gtggccctcc gtcgggctgt agttcacgcc
      481 ggctctctgg tagggcggaa tgtccacgat tttcaactga gcgtctcctg tgtgaatcga
      541 cttcgtacgg tgttcaacga tattacaccg catgcctgcg ggttaaccgc acgtggaaac
      601 gctccggtcg attgacctgc gtgcagcgct ggcgatataa gtgaagcgct acgatcgcgc
      661 ccggacggtg tctcatggag ccttgtttcc tgactgctgc tgccgcctcc aacctgatac
      721 gggaagggaa gctgacgagc gaagcgctcg tgcgctcgtg cctggaacgc atcgcggccc
      781 gcgatggcga cgtccgcgcc tggtcgtatg tggatccggc acaggcgatt gagcaggcac
      841 gcgagctcga caagcgcgcg ccgctcgggc cgctgcatgg tattccattc ggcgtgaagg
      901 acgtgatcga cacggcggat atgccgacga cgcagaactc gccgcactat cagagtcatc
      961 atcctgccaa ggacgcaggc tgcgtggcgg tcgtccggca ttccggcgcg ttgatcctcg
     1021 ggaagacgga caccgtggaa ttcgccgcgg gcgggcgcaa ggcgctcacg cgcaacccgc
     1081 acaacctcgc ccacacgccc ggcggatcgt cttcgggatc gggcgccgcg gtcgcggatt
     1141 tcatggtgcc gcttgcattc ggcacgcaga cgggcggatc gcagatccgc ccgtcctcct
     1201 tcaacggcgt ctatgcgctg aagccaacct ggggcgcggt ttcgcgcgag ggcgcgaagc
     1261 tttgctcggc gtactgcgac accgtgggct ggtacggccg ctcggtggcc gacctcgcgc
     1321 tggtggccga agcattccgg ttgcgcgatc tgggcgcgca gcaaccggtg gccgtgaacg
     1381 ggctgcgcgc cgcggtatgc agaacgccct tctggaacaa ggcggaacca ggcgcgcaga
     1441 aggcgctctc ggcggctgcc gaacgcctgg aagcagccgg tgtgcgcgta cgcgcactcg
     1501 agctgccggc caagttcgct ggcatgaacg aagcgcaacg cacgctgatg cacggcgaag
     1561 gaggggctgc attcctgccc gagctgctgg cgttcggcga gcggctgcac gcggaatttc
     1621 gtgacatggc gaccaacaaa cgcggcataa cagccagaat gatggtcgag gcctacgacc
     1681 tggcagcaga ctgcgcgcgt actttcgatg ccatgttcga cgagttcgac ttcgtcgtcg
     1741 caccagcggc gcccggggaa gcgcccgagg gcctgagcac caccggcgat tgggtattca
     1801 acgcgatgtt cacggcgatg cacgtgccct gtctcgcgat tccctgcacc cggggcgaaa
     1861 agaacctgcc ggtcggcgtg cagatcgtcg gaccgcgctt ttccgatgca cagctccttg
     1921 caatcgcagc agccgtggcc cccgtggtcg acgccgagaa acgaccatga caggcgcaag
     1981 gctccccgca tgctgtcgcc tgcgcagcgg tctgcgcgtc gggcagggtg cgggaagacg
     2041 cgccttgctc atggcatcct ggcggagcgt gctgcctgcc ttggcccttg cgatcgctct
     2101 gcacagcacc gccacgccgg ccgcggattc cgcggtgacg cagtacccca cgcggccgat
     2161 ccgcctgatc gtcgggtttc cgccgggctc gaccgacgac tacaacgcac gcgtgctcgc
     2221 gccgaagctc accgagcgtc tcggtcagca ggtcg
//
LOCUS       DASURG010000221         5287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_244263, whole genome shotgun sequence.
ACCESSION   DASURG010000221 DASURG010000000
VERSION     DASURG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244263"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFY80_03760"
     CDS             complement(<1..293)
                     /locus_tag="VFY80_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007052.1"
                     /translation="MNPILVSVSGCLALALLPLTLRAAPASGVPVGYPNKPIRFISGA
                     PGSPPDVAARIIMERVGGSLGQAIVIDNRPGAIGTIGLHAVAKASPDGYTFGA"
     gene            complement(428..742)
                     /locus_tag="VFY80_03765"
     CDS             complement(428..742)
                     /locus_tag="VFY80_03765"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEX6007053.1"
                     /translation="MITALVQFKLPQPVTRGKAQEIFLSTAPRYRNTPGLLRKYYVLS
                     PDGGTAGGIYLWQSRKDAEALYTKEWQDTVRERYGAPPSITFFESPVIVDNVTNEIVS
                     DS"
     gene            1184..1972
                     /locus_tag="VFY80_03770"
     CDS             1184..1972
                     /locus_tag="VFY80_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6007054.1"
                     /translation="MSNELSGKVAVIAGGSRGIGRAIGKALAEHGAQTVLAAKSEVNL
                     KKAADEIAATSAPKPTIVAADLGTADGCRAVFEHVKKTYGRCDILINSAGATQAGNFL
                     EQSDEIWMDGFALKFFGAVRLSRLFWPMLSAAQGKIVNIDGGMARTPNPGNLIGSAVN
                     AAMASFSKGLSSLGIRDGVNVNTIHPGRTETDRNAELVKQQAAAEGKTVEQVAAETAK
                     KAGVRRLGQPEDVAALALFLVSPAASHIQGAAISVDGGATKGLF"
     gene            2095..2910
                     /locus_tag="VFY80_03775"
     CDS             2095..2910
                     /locus_tag="VFY80_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6007055.1"
                     /translation="MPIIDSQIHPYEANTPKRPWQSTPNWPPSATADENIAAMDKLGI
                     DGAILISAFSIYRYDAAYAVEAYKAHPDRFALVKPVDPDDPAVADVIAEWKKTPGTVG
                     VRIQITKEPGRDRDPNHPGFERIARAAARHDFPLNILCWGNIDAGAAIIDRFPETRFI
                     IDHLAILQPRTPPAPPNAWADLPKVLELAKRDQGEWRLHALSRTVSVPGYLGSACAHF
                     RRVGVRSLPLGHGLDARQCGRQLRAGHPALPGDQPPERKRARDAHGRCLRKGV"
     gene            complement(3086..4327)
                     /locus_tag="VFY80_03780"
     CDS             complement(3086..4327)
                     /locus_tag="VFY80_03780"
                     /inference="COORDINATES: protein motif:HMM:NF023520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HEX6007056.1"
                     /translation="MRRLLAYLATLFAALTTGLVWSPSAFGRLIQEDLYFGRNIPGGG
                     EVSESQFQTFIDGVITPRFPAGLTTFDANGQFRDSTGTTIQERSKVVTLFVDDASAPQ
                     ASIDAIAAAYRQQFRQESVLQVGNRDDLQVSFGEGDLFANGSTPKLIQTDLYFGRNIP
                     GAGQVSESQFQMFVDSVITPSFPAGLTILDAQGQFQDSTGTVIKEPSKLVTLVLDDTL
                     TNESSVNQIIREYIQQFNQESVLVAANEAVTVSFGTSPDLFDNSSVSKLIQTDLYFGR
                     NISSGGMVSEGEFQMFLDAVVTPLFPAGLTVFDAQGQFLSSSGTLIKEASKLLTLIFE
                     DTLVNEGFVNEVIDTYLDRFNQESVLSVFDPDVAVSFLATANVPEPSTLVLLSFILLL
                     LLFAAAHKEILARRVYNASER"
     gene            complement(4544..5173)
                     /locus_tag="VFY80_03785"
     CDS             complement(4544..5173)
                     /locus_tag="VFY80_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HEX6007057.1"
                     /translation="MHQWQEIIETESDIRRILGEPSESQQTKCIDHIDKHCRVWIERC
                     PFVLVCSADASGMMDVSPKGDPAGFVRVLDDRTLIVPDRRGNRRADTFLNVLQNPNVG
                     LIFLIPRRGETLRVSGKARIVRDDALLAGMAMSGKKPDLALEITVSQAMFHCGKSMIR
                     SKLWNSEHWPSIEGLPSYAQALADQSKTSETIEEMELKIANFEQRGTLY"
BASE COUNT         1126 a         1564 c         1594 g         1003 t
ORIGIN      
        1 aacgcgccaa acgtatatcc gtccggcgag gctttggcca ccgcatgcag tcctatcgta
       61 ccgatcgcac ccggacgatt atcgatcacg atcgcctgcc ccaggctgcc gcccacgcgc
      121 tccatgatga tgcgggcagc cacgtccgga ggcgagcccg gcgcgccgct gatgaagcga
      181 atcggcttgt tcggatatcc gactggaaca ccggaggccg gcgctgcgcg caaagtaagc
      241 ggcagcaaag ccagcgcgag gcagcctgac actgacacaa gtatagggtt catgcgtgag
      301 tcagccctct ttgcctgatt cttcgcctgt accaagtgcg cgcttctccc tacctatcct
      361 gcatgatggc ctgccaagcg gcacgtcctg tagtgttggg ctgaaagcgg cgatcagccg
      421 cgagcgctca cgagtccgaa acgatctcgt tcgtcacgtt gtcgacgatg acagggcttt
      481 cgaagaaggt gatggacggt ggcgctccgt accgctcgcg aacggtgtct tgccattcct
      541 tcgtgtaaag agcctcggca tccttgcggg attgccagag gtagatacca cctgcagtgc
      601 ctccgtccgg agaaaggacg tagtactttc gaaggagccc gggggtgttc cgataccttg
      661 gcgcagtgct gaggaagatc tcctgcgctt tcccacgagt gacaggttgc gggagcttga
      721 actgaaccag agcggtgatc atggtgattc cctccttggg ttcgttcgac gcctcgacga
      781 ctgcgattac tccatgacgg caaacggtac ttatacgggc gctggcgccc cggatacgat
      841 ttgctcctgc tcgcacccgc cggtgcactg aaagaggcaa attccttagc attgtgtcat
      901 tgcggtgtcc gtataacaac gcgaatatgt gggcagccga cgaagcgagc gcgacgaggg
      961 tttaccgacc agccaggatt ggcctatttg aggcaaggat tcgtaacagc cgtcgcagct
     1021 ctggcgtagc tcgcgaacat ccgcgttccg tagcgccagt ggcagcaacg gggcgggact
     1081 agtggtcaaa atgcgccaca tgacgcggtt ggtgcgggta ccataaggac gaaagcagct
     1141 ttggtttcta cgccgagcag gctcatctgg aaggagcaaa gccatgtcga atgaattgtc
     1201 cggcaaagtt gcagtgatcg cgggcggcag ccgcggcatc ggccgcgcca ttggcaaggc
     1261 tcttgccgag cacggagcgc agacggtgct ggccgccaag tcggaagtca acctcaagaa
     1321 ggccgccgac gagatcgccg cgaccagcgc gccgaagccg accatcgtag ccgccgacct
     1381 cggcacggct gacggttgtc gggcggtatt cgaacacgtg aagaagacgt acggccgctg
     1441 cgacattctg atcaactcgg ctggcgcaac ccaggccggc aatttcctcg aacagtccga
     1501 cgaaatatgg atggacggtt tcgcgttgaa gttttttggc gccgtgcgcc tgtcgcgcct
     1561 gttctggcca atgctgagcg ctgcgcaggg caagatcgtc aatatcgatg gcggcatggc
     1621 gcgcacgccc aatcccggca acctcatcgg tagcgcggtc aatgcggcca tggccagttt
     1681 cagcaagggg ttgtccagcc tcgggatccg cgatggcgtc aacgtcaata ccattcaccc
     1741 tggccgcact gaaaccgacc gcaatgccga gctcgtcaag cagcaggccg ccgccgaggg
     1801 caagacggta gagcaggttg cagccgaaac ggccaagaag gccggcgtcc gccgcttggg
     1861 ccagccagag gacgtcgcgg cgctggcgct cttcctggtc tcaccggcgg ccagtcacat
     1921 ccagggcgcg gcgatctcgg tcgacggggg cgccaccaag gggttgttct gaccgtcatc
     1981 gaacgacatg ccgggaaacc cgtccttcca tctcgcgggt catggcgcgg aattgaagct
     2041 gctgactagg cagcagccaa cagcctgagc tgcgacgtca cggagacgac attcatgccc
     2101 atcatcgatt cccagattca cccgtacgaa gccaacacgc cgaagcggcc atggcaaagc
     2161 acgccgaact ggccgcccag cgcgaccgcc gacgagaaca tcgcggcgat ggacaagctc
     2221 ggcatcgacg gtgcgatcct gatctcggcg ttctccatct accgctacga cgcagcctat
     2281 gcggtcgaag cgtacaaggc gcatccggat cggttcgcgc tcgtgaagcc ggtcgatccg
     2341 gacgacccgg cggtcgccga cgtgatcgcg gagtggaaga aaacgccggg gacggttggc
     2401 gttcggattc agatcacgaa agagccgggc cgcgaccggg atccgaacca tcctggcttc
     2461 gagcgcatcg cgcgggcagc cgcccgccac gatttccccc tgaacatcct gtgctgggga
     2521 aatatcgacg ccggcgccgc gatcatcgat cgctttccag agacccggtt catcatcgac
     2581 cacctcgcga tcctgcagcc gcgtacaccg cccgcgccgc cgaatgcgtg ggccgatctt
     2641 ccgaaggtgc tcgagctcgc gaagcgtgat caaggtgagt ggcgcctgca cgctctctca
     2701 cgaaccgtat ccgtacccgg atatctggga tccgcttgcg cgcattttcg acgcgtgggg
     2761 gttcgatcgc tgcctttggg gcacggactg gacgcgcgcc aatgcggtcg tcaacttcga
     2821 gcaggccacc cggcccttcc tggagaccag ccgcctgagc gaaagcgagc gcgcgatgct
     2881 catgggcggt gcttgcgcaa aggcgtatag ctggtcgccg acagggcggg cgccgcgctg
     2941 agcacggccg ctattgcgtc aacggcgtca aagcagagcc gtggcgtcag cgtatgcagc
     3001 gacgtacgac caaggggcga tctgaaagtc agctgaccag acgtcaagta ccgccgcgca
     3061 tgcatcgcgg aacgctggtg atgcttcagc gctcggaggc gttgtacacc ctgcgcgcaa
     3121 gtatctcttt atgggccgct gcgaagagaa gcaacagcaa aatgaaagat aaaagcacca
     3181 gtgtagaggg ctctggaaca ttcgccgtcg ccaggaagct cactgcgaca tccggatcga
     3241 acactgagag gacactttcc tgattgaacc ggtcgaggta cgtatcgatg acctcattca
     3301 caaaaccctc gttgaccaga gtgtcctcga aaataagggt aagcagcttt gatgcttcct
     3361 tgatcagggt gccgctgctg ctgaggaatt gcccctgggc atcgaaaact gtcaagccag
     3421 cgggaaaaag aggcgtgacc acggcatcca gaaacatctg aaactcgccc tcggatacca
     3481 ttccgccgct agagatgttc cgcccgaagt acagatccgt ttggatcagt ttcgaaacgg
     3541 aggagttatc gaaaagatca gggctcgtgc cgaagctgac cgttacagcc tcattggcgg
     3601 ctaccagcac gctttcctga ttgaactgtt gaatgtattc ccgaatgatc tggttgacag
     3661 agctttcatt ggtgagggtg tcgtccagga cgagagtgac aagcttggac ggctctttga
     3721 tgacagtccc ggtactgtcc tggaattgac cctgggcgtc gaggatcgtc aatccggccg
     3781 gaaaactcgg agtgatgacg ctatccacaa acatctgaaa ctggctctcg gatacttgtc
     3841 cggccccggg gatgttgcgc ccgaaataca agtccgtctg gatcagcttt ggagtcgagc
     3901 cattcgcaaa cagatcaccc tcgccaaagc tcacctggag atcgtctctg ttccccacct
     3961 ggagcacgct ctcttgtctg aactgctggc gataggccgc ggctattgca tcgatggaag
     4021 cctggggtgc ggaagcatca tcgacgaaaa gggtaacaac cttggagcgc tcctggatcg
     4081 tggtcccggt gctgtcgcgg aactggccat tggcgtcgaa agtcgtcagt ccagcaggga
     4141 atcggggagt aatcacacca tctataaatg tctgaaactg gctctcggat acttctccgc
     4201 cgccagggat attgcgtccg aagtacaaat cctcctgaat gaggcggccg aaggccgacg
     4261 gactccaaac gaggccggtg gtgagggcgg caaaaagcgt cgcaaggtat gcaagcaatc
     4321 ggcgcatgag aaggctcctt tgacgagaca aggggagcgg cgagctttta gacgagcccg
     4381 gtattaacgg cgtgttgtga aaaacagcgc cgtacacgtg aacgaagatt cactgctcat
     4441 gcgttaatcg cctttgcgga aaatcggcga tacggcaact ggctgtcgcg tgatcttctg
     4501 gcaatcaatg cgtaaatggt gcgctcgatt ccgtgcctga aaactaatac agcgtgcctc
     4561 gctgctcgaa attggcgatc ttgagctcca tctcttcgat ggtctcgctc gttttggatt
     4621 ggtccgcaag cgcctgcgcg tagctcggta acccttcaat ggacggccag tgctcggaat
     4681 tccagagctt ggagcgaatc atcgatttgc cgcaatggaa catcgcctgg gaaaccgtga
     4741 tctccaacgc caagtcgggt ttcttgccgc tcatcgccat cccggccaga agcgcatcgt
     4801 ctcgcacaat gcgcgccttg ccggagaccc gaagcgtctc gccgcgcctc ggtatgagaa
     4861 agatcaaccc gacattcgga ttctggagca cattcagaaa ggtgtcggcg cgtcggttgc
     4921 cgcggcgatc cggcacgatc agcgtccgat catccaggac acgcacaaag ccggcgggat
     4981 cgcccttggg cgaaacgtcc atcatcccac ttgcatccgc gctgcagacg agcacgaacg
     5041 gacaacgctc tatccagacc cgacaatgct tatcgatgtg gtctatgcac ttcgtttgtt
     5101 gggactctga tggctcaccc agaatcctgc gaatgtccga ttccgtctca ataatctcct
     5161 gccactggtg catggtctac acctctagtg gagccacgag ccgagacttt cgacaagcat
     5221 gttgatgcag ggcttgcaga tgcatcatag gcttggggaa gcccgacgag caaggcgagc
     5281 gtagagc
//
LOCUS       DASURG010000222         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_141149, whole genome shotgun sequence.
ACCESSION   DASURG010000222 DASURG010000000
VERSION     DASURG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141149"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..966
                     /locus_tag="VFY80_03790"
     CDS             148..966
                     /locus_tag="VFY80_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX6007058.1"
                     /translation="MGGIAIAGSRGGTFAACLTTLLFVFAATTACAQEGIPSGATGGT
                     SSEGANAQLQRCDETFGTLAVHEDQAAPWWRQYYSRYPSLGSTTPVLRMLIQQSNCFV
                     VVERGQAMSNVMQERALQQTGELREGSNFGKGQMVSADYTMSPSIQFSQKGTSGLGGA
                     IGGLFGGVAGAIVGGLRSNEAATTLLLIDNRSSVQIAAAVGSAQNFDFSLFGGAFGGG
                     AGGAAGAFTNTPEGKILTAAFADSYNKMVQALRNYKAQQVRGGLGKGGNLKVGE"
     gene            complement(1306..1382)
                     /locus_tag="VFY80_03795"
     tRNA            complement(1306..1382)
                     /locus_tag="VFY80_03795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Arg,seq:ccg)
     gene            1491..2066
                     /locus_tag="VFY80_03800"
     CDS             1491..2066
                     /locus_tag="VFY80_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6007059.1"
                     /translation="MSEEIHVEEHSSPIRTPKQLVIVVVLAFTVPIILIALIAHLATL
                     SVDSAVDNPGMSEEAIAKRLRPVGEVAMMTEEERKARAAAAKVAPPPPPQPAAGAQAA
                     APVQSAAGDAGKGKTVYDSACAACHATGVAGAPKFGDKAAWAARIKSGMDTLYASALK
                     GKGAMPPKGGNPGLGDAEVKAAVDYMVGPVK"
     gene            2137..2964
                     /locus_tag="VFY80_03805"
     CDS             2137..2964
                     /locus_tag="VFY80_03805"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX6007060.1"
                     /translation="MSTYVIGDVQGCYDQLLRLLEAIRYDDARDTLWFVGDLVNRGPQ
                     SLAVVRFVKRLGARAVTVLGNHDLALLVVAEGLKRQHASDTFGDILVAPDRCELLDWL
                     RHRSMMHFDGGHAMVHAGLLPQWSVPTALALGGEVELALRDPDYREFLRHMYGNSPDR
                     WDDALSGFDRLRVITNAMTRLRLCTPEGRMEFSHKLGLENAPNGYLPWYDVPRRASRD
                     TPLIFGHWAALGLFVRDDVIGLDTGCVWGRSLTACRLEDRRIEQCDCGEMTGRAGED"
     gene            complement(2971..3906)
                     /locus_tag="VFY80_03810"
     CDS             complement(2971..3906)
                     /locus_tag="VFY80_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX6007061.1"
                     /translation="MRTLICGSIAYDTIMVFRDRFRNHILPDQLHILNVAFLVPDMRR
                     EFGGCAGNIAYTLSLLGGEPLVMATVGDDHEPYSRRLRDLGLAQDHVRAIGGTYTAQA
                     FITTDLDDNQITAFHPGAMNHSHENHVYEARDIRLGIVAPDGREGMLRHVQEFYAAGI
                     PFVFDPGQGLPMFRGEELLEFLRMADYVTVNDYEARLLQERTGATLDQLAKQVKALVV
                     TLGARGSVIRTASGEMEIPGVTAEKIVDPTGCGDAYRAGLLYGIASGMDWPLTGRLAS
                     LLGALKIASRGGQNHRFSRDEIAARFRENFGTNPW"
     gene            3998..>4242
                     /gene="mscL"
                     /locus_tag="VFY80_03815"
     CDS             3998..>4242
                     /gene="mscL"
                     /locus_tag="VFY80_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX6007062.1"
                     /translation="MPMLKEFKEFAMRGNVVDMAVGIIIGAAFGKIVDSLVKDLIMPP
                     VGMLLGKVDFSNLFIVLKQGTTSGPYLTVDAAQKAGAV"
BASE COUNT          792 a         1297 c         1372 g          781 t
ORIGIN      
        1 tccccgatta gatcgctgcc cgcatgccag cggattaggc ggcggtaggc tcgacgctgg
       61 cacggctcaa gcaaattttg tatagtaggt cgcagccggg agggtttccc ggtgccgcag
      121 cgagcctgac ttttcaggga ggagaccatg ggaggaatcg cgatcgcagg atcgcgcggc
      181 ggaacgttcg ccgcgtgcct caccacactg ctgtttgtct ttgccgctac gaccgcttgc
      241 gcccaggaag ggatcccctc gggcgcaacg ggcggcacca gttcggaagg cgcaaacgcc
      301 cagctgcagc ggtgcgacga gacgttcggc acgctcgccg tgcacgagga ccaggctgcg
      361 ccctggtgga gacagtacta cagccgttat cccagtctcg gcagcaccac gcccgtcctg
      421 cgcatgctga tccagcagtc gaattgtttc gtcgtggtcg agcgcggcca ggcaatgagc
      481 aacgtgatgc aggagcgcgc gttgcagcaa acaggggagc tgcgtgaagg cagcaacttc
      541 ggcaagggcc agatggtctc cgccgactac accatgagcc cgagcatcca gttttcccag
      601 aaaggcacga gcgggctggg cggcgcgatc ggcgggcttt tcggaggcgt tgcaggtgcg
      661 atcgtcggcg gtttgagaag caacgaggcg gccaccacac tgctgctgat cgacaatcgt
      721 tccagcgtgc agattgcagc ggcggtcggg agcgcccaga acttcgactt cagtcttttc
      781 ggcggcgcat tcggcggcgg tgcaggcggc gccgccggcg cctttaccaa cactccggaa
      841 ggcaagatac tgaccgctgc gttcgccgat tcgtacaaca agatggtgca ggccttgcgc
      901 aattacaagg cgcaacaggt gaggggcggc ctcggcaagg gcggcaacct gaaggtcggg
      961 gaatagcacc ccaagccgtg cgccaggcca gcgacatcgc tggcctttac gtcgcctgca
     1021 tggtccgggc gctgcaaagg cgcccggtta gttcctatac aacaatcctt accaagcctc
     1081 tcgctgaggt ctcagcaaca ttggcttttg ttccgaatca agcacgctcg tgctgcgcac
     1141 tttctcgctg aatccctgta ggctggactc ggacatcagg tcaggctgca tcgaaagcgg
     1201 cgaaagcccc tctctggttc aaggtcgaac aacgcgacga ctatcctgac acagggggcc
     1261 ggtataaaac gtcacctatg tgtccggtcc ggacctttga atgagtggcg cgcccggcgg
     1321 gagtcgaacc cacgaccttc ggcttcggag accgacactc tatccaactg agctacgggc
     1381 gcgtggcgtg ccgcaattct accacgccgt actaagggct catgaatcaa gcttttaccg
     1441 cgcaaggctt gccgctataa tgagcgcctt tttacgtgca ggaatccggg atgagcgagg
     1501 agatacacgt cgaggaacat tcttccccga tcaggacgcc gaagcaactc gtcatcgtcg
     1561 tcgttctcgc gttcactgta cccatcatcc ttattgcgct catcgcacac ctcgcgacct
     1621 tgagcgtcga ttcggcagtc gacaatcccg ggatgtccga agaagccatc gcaaagcggc
     1681 tgcggcccgt cggagaagtt gcgatgatga cggaggaaga acgcaaggct cgagctgcgg
     1741 cagcaaaggt cgcgccgccg cccccgcccc aacccgccgc cggcgcgcag gcggccgcac
     1801 cggttcagtc cgccgccggc gacgcgggca aaggcaagac agtgtatgac agtgcatgcg
     1861 ccgcatgtca cgccacgggc gtcgcgggcg ctccgaagtt cggcgacaag gcggcgtggg
     1921 ccgcccgcat caagtccgga atggacacgc tttacgcgag cgctctcaag ggcaaaggcg
     1981 caatgccccc caagggaggc aatcccggac ttggggatgc tgaggtcaag gccgcggtag
     2041 attacatggt gggcccggtg aagtaactcc ggccggagat cttgggaggc gcagcatgct
     2101 gcgccttttt ttgtctgcag ccgacgaata ttccgcatga gcacctatgt gatcggcgac
     2161 gtgcagggct gctacgatca gctgctgcgg ctgctcgaag cgatccgata cgacgacgcg
     2221 cgcgataccc tgtggttcgt cggcgatctc gtcaatcgcg gtccacagtc gctcgcggtc
     2281 gtgcgcttcg tcaagagact gggcgcgcgc gccgtgacgg tcctcgggaa tcacgatctt
     2341 gcccttctcg tcgtcgccga aggcctcaag cgccagcatg cctccgatac tttcggcgac
     2401 atccttgtgg cgcccgaccg ctgcgagttg ctcgattggc tgcgtcaccg cagcatgatg
     2461 catttcgacg gcggtcacgc gatggttcac gccggtctgc tgccgcaatg gagcgtgccg
     2521 accgcgcttg cgctcggcgg tgaagtcgag ctggcgctcc gcgatccgga ttaccgtgaa
     2581 tttctgcggc atatgtacgg caattcgccg gatcgctggg atgacgcgct cagcggcttc
     2641 gaccgtttgc gcgtgatcac gaacgcgatg acacggctgc gcttgtgcac gccggagggc
     2701 aggatggagt tttcccataa gctcggtttg gagaacgccc cgaacggcta tctgccgtgg
     2761 tacgacgtgc cgcgacgcgc gagccgcgac acgccgctca tcttcggtca ctgggccgcg
     2821 ctggggcttt tcgtgcggga cgatgtgatc gggctcgata ccggctgcgt gtggggtcgt
     2881 agccttacag catgccgcct cgaggaccgc cgcatcgagc aatgcgactg tggcgagatg
     2941 acaggtcggg cgggcgagga ctgaagcgtc ctaccagggg ttcgtgccga agttctccct
     3001 gaagcgggcg gcaatctcgt cacgactgaa gcggtggttc tggccgccgc gcgaggcgat
     3061 tttaagagcg cccaggagcg acgcgagccg cccggtgagc ggccaatcca tccctgacgc
     3121 gatgccatag agcagcccgg cgcgatacgc gtctccacag ccggtgggat ccacgatttt
     3181 ttccgccgtg actccgggta tctccatctc tcccgacgcc gtgcggatga cggagccgcg
     3241 cgcgccgagc gtcaccacca acgctttcac ctgtttcgcg agttgatcga gcgttgcgcc
     3301 ggtgcgctcc tgcaggagcc gcgcttcgta gtcgttcacc gtgacgtagt cggccatgcg
     3361 caaaaattcg agcagttcct cgccgcggaa catgggcagc ccctgaccgg ggtcgaagac
     3421 gaacggaatg ccggcggcgt agaactcctg cacgtgccgc agcatgcctt cccgcccgtc
     3481 cggcgcaacg atgccgagcc ggatatcgcg cgcctcgtac acatgatttt cgtgggagtg
     3541 attcatggcg ccgggatgga aggcggtgat ctggttgtcg tcgaggtccg tggtgatgaa
     3601 cgcctgtgcg gtataggtcc cgccgattgc tctgacgtga tcctgcgcga gccctagatc
     3661 ccgcaggcgc cgcgagtacg gctcgtgatc gtcgcccacc gtcgccatga cgagcggttc
     3721 cccgccgagc agcgacagcg tataggcgat gttgccggca cagccgccga attcgcggcg
     3781 catgtcgggc accagaaaag cgacgttgag aatgtgaagc tgatcgggaa ggatatggtt
     3841 acgaaaacgg tcgcgaaaca ccattatggt gtcataggcg atggaaccac agatcagcgt
     3901 tcgcataacg ttgggcggca ggcggtgtaa gattatagcc gctcgccggc agaatccgcc
     3961 gtgctgcgcc gatccttaag gacgtttacc aagggagatg cccatgctga aggagttcaa
     4021 ggaatttgcg atgcgcggca acgtcgtcga tatggcggtc ggcatcatca tcggggccgc
     4081 cttcggcaag atcgtcgatt ccctagtgaa ggacctcatc atgccgccgg tcgggatgct
     4141 tctgggtaaa gtcgatttct cgaacctctt tatcgtgctc aagcagggca cgacgtccgg
     4201 cccgtatctt accgtcgatg ccgcgcagaa ggcgggggcg gt
//
LOCUS       DASURG010000223         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_244446, whole genome shotgun sequence.
ACCESSION   DASURG010000223 DASURG010000000
VERSION     DASURG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244446"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="der"
                     /locus_tag="VFY80_03820"
     CDS             <1..834
                     /gene="der"
                     /locus_tag="VFY80_03820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX6007063.1"
                     /translation="EAGEAAEVGEKHPKVAIVGRPNVGKSTLANALLGEERVLVFDQP
                     GTTRDAIYIDFERGGRRYTLIDTAGLRKRGRVEEAPEKFSVVKTMQAIADANVVILVL
                     DARQDISEQDAHIAGFILEAGRALVVAVNKWEGLDEDQRDLVKRALARKLSFLDFARL
                     HFISALAGTGIRALMNSVDAAYQAAMARLPTPRITRALQAAVSRQQPPRAGRVRPKLR
                     YAHQGGANPPRIVVHGTALEHIPDSYRRYLERFFRDAFKLQGTPLKVEFRSGRNPYLK
                     A"
     gene            925..1161
                     /gene="hfq"
                     /locus_tag="VFY80_03825"
     CDS             925..1161
                     /gene="hfq"
                     /locus_tag="VFY80_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX6007064.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHIPVSIYLVNGIKLQGQIDSFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVNFQLDQGAD"
     gene            1189..2319
                     /gene="hflX"
                     /locus_tag="VFY80_03830"
     CDS             1189..2319
                     /gene="hflX"
                     /locus_tag="VFY80_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX6007065.1"
                     /translation="MFDRPGHGTEAVLVSLDFGEPDPAESLEEMQQLVASAGVAARAV
                     VKGKRQKPDSALFAGRGKVAEISESVFAHRAPLVIFNHDLSPVQERNLEQKLQCRVVD
                     RTSLILDIFAQRARSHEGKLQVELAQLEHLSTRLVRGWSHLERQKGGIGLRGPGETQL
                     ETDRRLLAKRVKVLKEKLAKVRRQREVQRRARSRANVLSVSLVGYTNAGKSTLFNRLT
                     RSDAYAADQLFATLDTTTRRMHLPNGSAVVLSDTVGFIRDLPHTLVAAFRATLEETAR
                     ADLLLHVVDASSATRDAQIAAVNEVLSEIGAEDIPQVLVYNKIDRTRLPARSERDQYG
                     RIARAWISARSGDGLSLVRLALEEHAAGTTSRSETITSPAAA"
BASE COUNT          469 a          786 c          784 g          420 t
ORIGIN      
        1 gaagctgggg aggcggctga ggtcggcgaa aagcacccca aggtcgccat cgtcggacgg
       61 ccgaacgtcg gcaagtcgac ccttgccaac gcgcttctcg gtgaagagcg cgtgctcgtc
      121 ttcgaccagc ccggcaccac ccgcgatgcg atctacatcg atttcgagcg cggcggccgg
      181 cgctatacgc tcatcgatac cgcggggctg cgcaagcgcg gacgggtgga agaggcgccc
      241 gagaagtttt cggtggtcaa gaccatgcag gcgatcgccg atgcgaacgt ggtgatcctg
      301 gttctcgacg cccggcagga catttccgag caggatgccc atatcgcagg cttcatcctg
      361 gaagccgggc gggcgctggt cgttgccgtc aacaagtggg aaggcttgga cgaggatcag
      421 cgggacctcg tcaagcgggc ccttgcccgc aagctgtcgt ttctggattt cgcgcgcctg
      481 catttcatct cggccctggc cggcacgggc atccgggcgc tcatgaattc tgtggacgcc
      541 gcctatcagg cggccatggc gcggctgccg accccccgca tcacgcgggc gctgcaggcc
      601 gcggtatcgc gtcagcagcc gccgcgcgcc gggcgggtgc gtccgaagct ccgctatgct
      661 caccaaggcg gggcgaatcc ccctcgcatc gtcgttcacg ggacggcgct ggagcacatt
      721 cccgacagtt atcgccgcta tctggaacga tttttccgcg atgctttcaa attgcaaggc
      781 acgccgctca aggttgaatt cagaagcggg cgtaatccgt atctgaaggc gtgagcggta
      841 aactcggcta accctatggc ctaacggtga aatttaaagt aaagtgactg tttcagttcc
      901 aataacgaca acggcgaggg catcatgagc aacaaggggc aattactaca agacccgttc
      961 ctgaacactc tgcgcaagga gcacatcccg gtctcgatct acctcgtcaa cggcatcaag
     1021 ttgcaggggc aaattgactc gttcgaccag tatgtcgtcc tcctcaagaa cacggtgacg
     1081 cagatggtgt acaagcatgc gatctccacc gtcgttcctg cacggcccgt caactttcag
     1141 ctcgatcaag gtgccgacta aggcaatgtc ggatctcagc tgtaattgat gttcgaccgt
     1201 cccggccacg gcaccgaggc ggtgctggtc agtctcgact tcggcgaacc ggaccccgct
     1261 gaaagcctgg aagaaatgca gcagctcgtc gcgagtgcgg gggtggctgc gcgcgcggtt
     1321 gtgaagggca agcgccagaa accggactcg gcgctgttcg ccgggcgcgg caaagtcgcg
     1381 gagatttcgg agtccgtgtt cgcgcatcgc gctccgctcg tgatcttcaa ccatgatctc
     1441 tcgccggtgc aggagcgcaa tctcgagcag aagctgcaat gccgcgtcgt cgaccgcacc
     1501 agcctgatcc tggacatctt cgcgcagcgc gcccgcagcc atgagggcaa gcttcaggtg
     1561 gagcttgccc agctcgagca cctgtcgacg cggctcgtgc gggggtggag ccacctggaa
     1621 cggcagaaag gcggcatcgg cctgcgggga cccggcgaaa cacagctcga gaccgaccgg
     1681 cggctgctcg cgaagcgggt caaggtgctc aaggagaagc tcgccaaggt ccgccggcag
     1741 cgtgaagtgc agcgccgcgc gcgctcgcgc gccaacgtac tgtcggtatc cctggtggga
     1801 tacaccaatg ccggcaagtc gacgctcttc aaccggctga cgcgctcgga cgcctacgcc
     1861 gcggaccagc ttttcgcgac gctcgacacc acgacgcggc gcatgcatct tccgaacggc
     1921 agcgcggtgg tgctctccga caccgtcggg ttcatccgcg acctcccgca tacgctggtc
     1981 gcggcattcc gcgcgaccct ggaggaaacc gcacgcgccg atctgttgct ccacgtggtg
     2041 gacgcgagca gcgcaacgcg cgacgcacag atcgccgcgg tcaacgaggt gctgtcggaa
     2101 atcggcgccg aggacatccc gcaggtgctg gtctacaaca agatcgaccg cacccgcctt
     2161 cccgcgcgaa gcgagcgcga ccagtatggt agaatcgcac gtgcctggat cagcgccagg
     2221 tcgggggatg gtttgagtct cgtgcggctt gcgctggaag aacacgccgc cggcacaacg
     2281 tctcgctccg aaaccattac aagccctgcg gcagcctaga acgcaaagct ctttttcatg
     2341 ctcgctcatg caaggtcatt tcgcgactgg gtgaaggcgc gcgcgctgcg tctggcgggc
     2401 gccctcatgt cgatcaacga tccgcagtgg ggtaaacgcc ccggccgcga aggcccgcc
//
LOCUS       DASURG010000224         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_39719, whole genome shotgun sequence.
ACCESSION   DASURG010000224 DASURG010000000
VERSION     DASURG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39719"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VFY80_03835"
     CDS             complement(<1..819)
                     /locus_tag="VFY80_03835"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX6007066.1"
                     /translation="MKKEIQRIALSTGGGDAPGLNAVIRSAVLAALSRGWEVFGIRKG
                     YAGLLGEDTIIPLGRDSVRGISHIGGTILGTTNRGNPFEWPVRNDKGEMTTADRSDEV
                     IAAFERHGFDALISIGGDGSLSIADRLCKKGMPIIGVPKTIDNDLAVTEVTFGFNTAV
                     QTATEAIDKLHSTAESHERVMVLEVMGRTAGWIALESGMAGTADVILIPEIPYDIERV
                     CEKIEDRYEHGRRFAIVVVAEGAVPVDGAASFVETNVVGQANRYGGAAERLARDI"
     gene            complement(827..1000)
                     /locus_tag="VFY80_03840"
                     /pseudo
     CDS             complement(827..1000)
                     /locus_tag="VFY80_03840"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(1162..1551)
                     /locus_tag="VFY80_03845"
     CDS             complement(1162..1551)
                     /locus_tag="VFY80_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007067.1"
                     /translation="MRHTILPLAAALLWAAGSAQAQQPASAVRLEVGEWTGTVVPPEG
                     HPAAVVYDVAYAGDTLKIKIRAGEHGTFETWEVKAEGNKLSFKFRPGPEVVCVLEKKE
                     NAYAGQCTADDGSVASMDLAPPRKQKS"
     gene            1682..2674
                     /locus_tag="VFY80_03850"
     CDS             1682..2674
                     /locus_tag="VFY80_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007068.1"
                     /translation="MHSAKLLARRYACCLLLLAACGGDATQPGPPEDESDESGVPSLG
                     SNAALNGRRPFPADNPWNQDISNEPVDPNSATLIAACGMRNLHPDFGTAWNGAPNGIP
                     YVVVSGTQARVPVSFSYSSESDAGPYPIPPNPPIEGGPNGTGDRHILIIDRDNWRLYE
                     LFDAHPVNGGSSWQAGSGAIFDLNSNALRPAGWTSADAAGLPIFPGLVRYDEVVELQE
                     IRHALRFTCPTTRRAYVHPARHYASTRTDPNLPPMGMRVRLRADFDGSDYPAEVRVIL
                     HALKRYGMFLADNGSAWYISGAPDPRWNDDNLGALKTISSQNFEVVRMGEIITP"
     gene            complement(2720..>2906)
                     /locus_tag="VFY80_03855"
     CDS             complement(2720..>2906)
                     /locus_tag="VFY80_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007069.1"
                     /translation="MLRPSSRAKGEVAMQDAPSVAPEQALPVMTIVAIAAVVIVAVAL
                     IWWMRKTRGGRTPPRGS"
BASE COUNT          508 a          943 c          899 g          556 t
ORIGIN      
        1 gatatcacgg gccagccgtt cggctgcgcc accatagcga ttcgcctggc cgacgacgtt
       61 cgtttcgacg aaactcgcgg cgccgtcgac aggcacggca ccttccgcga cgaccacgat
      121 ggcgaagcgt cgtccgtgct cgtagcggtc ttcgatcttt tcgcaaaccc gctcgatgtc
      181 gtagggaatc tccgggatca gaatcacatc cgcagtgccg gccatgcccg attcgagcgc
      241 gatccagccc gccgtgcgtc ccataacttc cagcaccatg acacgctcgt ggctctcggc
      301 cgtggagtgc agcttgtcga tggcctcggt cgcggtttgc acggccgtgt tgaagccgaa
      361 ggtgacttcc gttacagcaa gatcgttgtc gatggttttc ggcacgccga tgatcggcat
      421 gcctttcttg cagagccggt ccgcgatact gagcgaccca tcgcccccga tcgagatgag
      481 cgcgtcgaag ccgtgccgct cgaaggccgc gatcacctcg tccgaccggt cggcggtggt
      541 catttcgccc ttgtcattgc ggaccggcca ctcgaacgga ttgccgcggt tggtggtgcc
      601 caggatggtg ccgccgatgt gcgagatgcc gcgcacggaa tcacggccga gcggaatgat
      661 cgtatcttca cccagcagtc ctgcatatcc tttgcggatg ccgaatactt cccagccgcg
      721 gctcaacgca gccagcaccg cggatcgaat cacggcgttg agcccgggag cgtcaccgcc
      781 cccggtgctc aacgcaatgc gttgaatttc tttcttcatc aattaatcag cgaagtttgt
      841 cgacgaggtc gttccacgtc gggtgcaggc cggtggcgcg tttcgagtag atcagctcgc
      901 catcgcgtga cacctcgaat acgccgccgc tcgagcgcac cagctcgatt tcggcatccg
      961 gaaagtcctg cttgatgcgc gcctccaact ggagagccct gggccggtag cctcaggagt
     1021 tgcagtactc gatacgaatg tgcatgactg atcgctcctt tcgccgcagg gtaaaggcag
     1081 cgtgcgcaac atgcaagaag cgccgcgccg ctcgcccttg cgggcgcgca gcgcggcgct
     1141 cttctgcggg tggtgaaccg ctcagctctt ctgtttcctg ggcggcgcga ggtccatgct
     1201 ggcgaccgag ccatcgtccg ccgtgcattg accggcgtag gcgttctcct ttttctcgag
     1261 cacgcagacg acttccgggc ccgggcggaa tttgaaggag agcttgttgc cctcggcctt
     1321 gacttcccat gtctcgaagg tgccgtgctc acccgcgcga atcttgatct tgagcgtgtc
     1381 gcctgcgtag gcgacgtcgt aaacaacagc tgcgggatgc ccttcggggg gaacgaccgt
     1441 acccgtccac tcgccgactt cgagtctgac cgcggacgca ggctgctgcg cctgggccga
     1501 accggcagcc cacagcaggg cagcagcgag tggaagaatg gtatgacgca tggggtctcc
     1561 aagtgaatgg tgtgctggct ttacggcggg cgcgcacacg cggtttcgcg taccgggcgg
     1621 tacgaaagct gccccggatg gcgtaacccg cgcctccccg cgccattggg tgaaaatcgc
     1681 catgcattct gccaaactgc tcgcgagacg ctatgcctgc tgtctgttgc tcctggccgc
     1741 atgcgggggc gatgcaacgc agccgggtcc gccggaggac gagtctgacg agagcggggt
     1801 tccctctctg gggagcaatg ccgcactcaa cggacggcgg ccgtttccgg ccgacaatcc
     1861 gtggaaccag gacatctcga atgagccggt cgatcccaac tcggctacgc tcatcgccgc
     1921 gtgtggcatg cgcaatctgc atcctgattt cggcaccgcc tggaacggcg cgcccaacgg
     1981 cattccttac gtcgtggtga gcggcacgca ggcgcgcgtg ccggtcagct tcagctacag
     2041 ctcggagagt gatgcggggc cgtatccgat tccgcccaat ccacccatcg aaggcggtcc
     2101 aaacggcacc ggtgaccggc acattctcat catcgatcgc gacaactggc gcctgtacga
     2161 gctctttgat gcacatccgg tgaatggtgg cagcagctgg caggcgggct cgggtgccat
     2221 cttcgatctg aacagcaacg ccctccgccc ggccggttgg acgtcagcgg atgcagcggg
     2281 gctgccgatc tttcccggtc tcgtacgcta cgatgaagtg gtcgaattgc aggagatcag
     2341 gcacgcgctg cgtttcactt gtcccacgac acgtcgtgct tatgtgcacc ctgcgcgcca
     2401 ttacgccagt acccgcacag acccgaacct gccgcccatg ggcatgcggg tgcgactgcg
     2461 tgccgacttc gatgggagcg attatccggc ggaagtgcgt gtgatcctgc acgcactcaa
     2521 gcggtacggg atgtttctgg ctgataacgg cagcgcgtgg tacatcagcg gtgcaccgga
     2581 tccgcggtgg aacgacgaca atctcggcgc attgaagacg atcagcagcc agaacttcga
     2641 agtcgtccgc atgggtgaaa tcatcacgcc gtaaatgccg atccctctgc gtcctcgccc
     2701 ctgcgcgtcc tggcttcact cagctgcccc gcggtggcgt gcgacctccg cgcgtcttgc
     2761 gcatccacca gatcaaggcc accgccacga tcacgaccgc cgcgatcgcg acaatggtca
     2821 tgacgggcag ggcctgctcc ggagcaaccg aaggcgcgtc ttgcatagca acctctcctt
     2881 tcgcgcgcga ggacggtcgc aacatt
//
LOCUS       DASURG010000225         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_244473, whole genome shotgun sequence.
ACCESSION   DASURG010000225 DASURG010000000
VERSION     DASURG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244473"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFY80_03860"
     CDS             complement(<1..267)
                     /locus_tag="VFY80_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6007070.1"
                     /translation="MQDHAAVTLIGGKFRWDDPFLIEEQLTDEERLICDTARQYAQEK
                     LAPRVRDWNRREHFDPMVMREMGELGFLGAQLDGYGCSGIGYVAY"
     gene            complement(281..1483)
                     /locus_tag="VFY80_03865"
     CDS             complement(281..1483)
                     /locus_tag="VFY80_03865"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX6007071.1"
                     /translation="MLNAYLYDGLRTPFGRHAGVLAKVRPDDLLAEVMKTVMTKSAFK
                     PEQIEDIVVGCANQGGEDSRCVARHAGLVAGLPIETPGTVLQRNCASGLGALVHAAHA
                     ITAGEGDVFMVGGVESMSRSPLVLSRSESAFGRDVKLYDSTIGPRFSNPKLVKRFGDD
                     QMPQTADTLAREYEIKREDADKFAHGSQQKYAIAKGEGFFAGEIAPVELPATRKGPVP
                     PVAEDEHPRPNTDLDTLAKMRPLYEGGVTTAGNASGVNDGAVALVVGTREAGEKAGVK
                     PLARVIASAAAGVPPQIMGIGPVAASKKALARAGLTVKDMDVIEINEAFAAQVLACLK
                     GLGVGFDDKRVNPNGGAIAVGHPLGASGARLALTAARQLQRTGGRYALVSLCIGGGQG
                     MAAILERV"
     gene            complement(1518..>2951)
                     /locus_tag="VFY80_03870"
     CDS             complement(1518..>2951)
                     /locus_tag="VFY80_03870"
                     /inference="COORDINATES: protein motif:HMM:NF006124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX6007072.1"
                     /translation="MRVLMTDSRPGAAQEARDFIARMLARAAEKGSIGKDEAGAAVGR
                     LTIADGPAAMRPCHVVIEAIVENLEAKRALFGELEGIVSPECILATNTSSLSVTTIAA
                     KLKTPQRFAGFHFFNPVPLMRLVEVIDGLQTENWVAEALMTVGRRMGREPVRCTDAPG
                     FLVNQVGRGFTLEAAHLVYEGISSFADVDRVMRDIGGFRMGPFELMELTGLDVTHPAS
                     ELIYRQFFEEPRYRPNLIMRARYEAGVLGRKSRKGFYDYDADMKPIVAPEPPPPAAYP
                     GNVWVSPAEPRGHSALVEMLRKLGASIETGPRPSANALILVTPIGDDATTCAVEQELD
                     PKRTVAVDTLFPLSKRRTIMGTPVTDHVYRNAAHGLLASDGVPVTVCRDSPGFIAQRI
                     VAMIVNIGCSIAQSRTAQPGDIDKAVTLGLNYPNGPLKFGDVLGAAQVHKVLASMYRI
                     YGDPRYRPNVWLTRRARLGVSLLTPEP"
BASE COUNT          511 a          969 c          916 g          555 t
ORIGIN      
        1 gtaggcgaca taaccgattc cggagcaacc gtagccatcg agctgcgcgc cgagaaaacc
       61 cagttctccc atctcgcgca tgaccatcgg atcgaaatgc tcgcggcgat tccagtcgcg
      121 cactcgaggt gccagcttct cctgggcgta ctggcgcgcg gtatcgcaga tcaggcgttc
      181 ctcgtcggtc agttgctcct cgatgaggaa cggatcatcc cagcgaaact tcccgccgat
      241 gagcgtgacc gccgcgtggt cctgcatgcg aagcggccgg tcagacccgc tcgagaatcg
      301 cggccatccc ctgcccgcca cctatgcaca ggctcacgag cgcatagcga ccgccggtgc
      361 gctgcagttg ccgcgcagcg gtcagggcaa gacgtgcgcc cgaggcgccg agcgggtggc
      421 cgactgcgat ggctccaccg ttcgggttca cgcgcttgtc gtcgaagccg accccgagtc
      481 ctttcaggca ggcaagcacc tgcgccgcaa acgcttcatt gatttcgatc acatccatgt
      541 ctttgaccgt gagcccggcg cgtgccaaag ccttcttgga tgccgctacg ggcccgatac
      601 ccatgatctg tggcggcacg ccggcggcgg cacttgctat gacccgggcc agtggtttca
      661 cgcccgcttt ctcacctgcc tcgcgcgttc cgacgacgag cgcgactgcg ccgtcgttca
      721 cgccggatgc attgcctgca gtagtcacac cgccttcata cagcggccgc atcttcgcga
      781 gcgtgtcaag atcggtgttg ggccgcgggt gctcgtcctc cgctactggc ggcacaggac
      841 ccttgcgggt ggcaggcagt tccactggcg cgatttcacc cgcgaagaaa ccttccccct
      901 ttgcgatggc gtacttctgc tgcgagccgt gcgcgaattt gtccgcatcc tcacgcttga
      961 tttcatattc gcgcgccagc gtgtcggcgg tctgcggcat ctggtcgtcg ccgaagcgct
     1021 tcacgagctt gggattggaa aagcgcggcc cgatcgtgct gtcatagagc ttgacgtcgc
     1081 ggccaaaggc gctctcggag cgactcagga cgagcggcga acggctcata ctctcgacgc
     1141 cacccaccat gaagacatcg ccctcgcctg cagtgatcgc gtgtgcggcg tgtaccagcg
     1201 cacccaggcc gctcgcgcag ttgcgctgaa gcacggtacc cggtgtttcg atgggcaagc
     1261 cagcgacgag gcccgcgtgc cgggccacgc aacggctgtc ttcgcccccc tggttggcgc
     1321 agccgacgac gatatcttcg atctgttcag gcttgaatgc ggacttggtc attacagttt
     1381 tcatgacttc agcaagcaga tcgtcggggc gcacctttgc cagcacaccg gcgtggcggc
     1441 cgaacggagt gcgcagtcca tcgtagagat aggcattgag catgatggaa ttcctctcgt
     1501 gaattcggga taaggactca gggctctggc gtgagcagcg agacgccgag gcgggcgcgc
     1561 cgcgtgagcc agacattggg ccggtagcgc ggatcgccgt agatccggta catcgatgcg
     1621 agcaccttat gcacctgcgc cgctcccagc acgtctccga acttgagcgg gccgttcgga
     1681 taattgagcc cgagggtcac ggccttgtcg atgtcgccgg gctgcgcggt gcgactttgc
     1741 gcgatagaac agccgatatt gacgatcatc gccacgatgc gctgtgcgat gaaaccgggg
     1801 ctgtcgcgac ataccgtgac aggcacaccg tccgaagcga ggaggccgtg ggcggcattg
     1861 cgataaacgt ggtccgtcac gggcgtgccc atgatggtgc gacgctttga cagcgggaat
     1921 aacgtatcca cggcgacagt acgcttggga tccagttcct gctccaccgc acaggtcgtc
     1981 gcatcgtcgc caatcggggt gacgagaatc aatgcattcg cgctcggcct cgggccggtt
     2041 tcgatcgatg caccgagttt ccgcagcatt tccaccagcg cgctatgccc gcgcggctcc
     2101 gccgggctca cccacacgtt gcccgggtaa gcagccggtg gcggcggttc cggtgccacg
     2161 atcggtttca tgtcggcgtc gtaatcgtag aaacccttcc tgctcttgcg ccccagcaca
     2221 cctgcttcgt aacgggcccg catgatgaga ttcggacggt agcgcggctc ctcgaagaac
     2281 tggcggtaga tcagctcgga agcgggatgc gtgacgtcga gcccggtgag ctccatcagc
     2341 tcgaagggcc ccatgcgaaa gcccccgatg tcccgcatca cgcggtccac gtctgcgaag
     2401 ctcgagatgc cttcgtagac cagatgagcc gcttcgagcg tgaatccgcg ccccacctgg
     2461 tttacgagaa agcccggcgc atcggtgcac cggaccggtt cgcgccccat gcggcgcccg
     2521 accgtcatga gcgcctcggc tacccagttt tcggtctgca acccgtcgat cacctccacc
     2581 agcctcatca gcggcaccgg gttgaagaaa tggaaaccgg caaagcgctg aggcgtcttg
     2641 agcttcgctg cgatggtggt taccgaaagc gaagacgtgt tggttgcgag gatgcattcg
     2701 ggtgagacga tgccttctag ctcgccgaag agcgcgcgct ttgcctccag attttcgacg
     2761 atcgcctcga tgacgacgtg gcacggcctc atcgccgcgg gcccgtcggc aatcgtgagc
     2821 cgccccacag ccgcacccgc ctcgtccttg ccgatactgc ccttctcggc ggcgcgggcg
     2881 agcattcttg cgataaagtc gcgcgcctcc tgtgcggctc cgggtcggct gtcggtcatc
     2941 aacaccctca t
//
LOCUS       DASURG010000226         5667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_39985, whole genome shotgun sequence.
ACCESSION   DASURG010000226 DASURG010000000
VERSION     DASURG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39985"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFY80_03875"
     CDS             complement(<1..579)
                     /locus_tag="VFY80_03875"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX6007073.1"
                     /translation="MGCCHAYFTQTGIGDDAFTIDASRLTFGAGCLKETGDHAKALGM
                     TRVGVYTDPRVRTLPHFDTVTSSLRAAGIDVAIYDAVKVEPTDASFKQAARFAQEGRF
                     DGFVSVGGGSVMDTCKAANLYSTHPADFLAYVNAPVGDGKPVPGPLKPHIACPTTSGT
                     GSEATGIAIFDLLALKAKTGIQSRWLKPTTGLI"
     gene            complement(595..2217)
                     /locus_tag="VFY80_03880"
     CDS             complement(595..2217)
                     /locus_tag="VFY80_03880"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX6007074.1"
                     /translation="MSAVASEAYDYVVVGAGSAGCVLANRLTEDGRHTVLLLEAGPED
                     RSLWIHVPIGYGKTMFHPVYNWGFYTEPEPAMNQRRVYWPRGRGLGGSSSINGLIYVR
                     GQREDYERWAALGNRGWGWNDVLPYFRKLERNTRGASEWHGDEGPVWCSDIGEPHALM
                     EAIIRAAEALGVPRNDDFNGASQEGVGYYQLFTRDGWRCSSATAYLRPALRRRNLRVA
                     TGAHVTGVVFDGLRATGVTYRQHGVDRSVRAVREVILAAGALQSPQLLQLSGVGRATL
                     LQDLGIPVVKHLPGVGENLQDHLQHRLIYKVRKPITTNDDLRTLAGKLRIGLRWLRHR
                     KGPLAIGINQGGLFTRVLPASTTPDVQFHFATLSADMAGGKPHKWSGCTFSVCQLRPE
                     SRGTVAINSRDPFEPPAMRPNYLTSEIDCRCAVEGIKFARRLAAQPALSEYIDEEYRP
                     GRGAVDDAALLAFARNHGATIFHPSGTCRMGDDAMAVVDARLRVHGLAGLRVVDCSIM
                     PRLTSGNTHAPVVMIAEKGADMIREEARHAGA"
     gene            complement(2235..3701)
                     /locus_tag="VFY80_03885"
     CDS             complement(2235..3701)
                     /locus_tag="VFY80_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX6007075.1"
                     /translation="MEQAMEPANLVFIMSDEHNKRVLGSYGHAMIRTPHLDRLAARGT
                     RFTNAYTNCPICVPARAAFATGQYVHRIRYWDNAIAYDGRIASWGHRLMAQGHRVTSI
                     GKLHYVDSDARRNGFDKEILPLHIVNGVGDLLGLIRDELPRRPGSAKLGPEAGRGESE
                     YTHYDRSIAREAVRWLKEEAPLYRDRPWALYVGFVSPHFPLIAPPGFYDLYPENEVPW
                     PDMYEHESRPRHPFLDAMRKCLCFDDPFDAPMVRRAITAYMGLVSFLDDNIGTILNAL
                     EESGLAASTRVVYSSDHGDNLGTRGLWGKSTMYEESAGIPMIMAGPDVPAGAACDTPV
                     TLVDAFPTIVEALGAEPDPQDAALPGHSLIRIAQGATPVRTILSEYHAAGAICGAYMI
                     RRGNYKYIHYVGLPPMLFDLERDPHERIDLGQEPSCRKIVSDCEAALRKVVDPEAADR
                     QARADQLAHIGKHGGKEAILKRGTFRYSPPPGVKAAYF"
     gene            3835..4611
                     /locus_tag="VFY80_03890"
     CDS             3835..4611
                     /locus_tag="VFY80_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6007076.1"
                     /translation="MGGFESLTLEVAVWAALVIAFAGFVQGALGLGFPMVATPLIAFT
                     TDMRTAVVWVLLPCLAAVAANVVKTGALREALARFWMMPFYMLIGAAIGTRLFIIAPQ
                     FPFSLLLAAMIILYLNLDRLGRARWTALETHRGFFAAVFGVAAGMSEGMANIAAPPLL
                     IYYLALGLSPAMLVQALNICFFVGKSTQFATLATVGGVPPVQWIATLPLVVVASVTVL
                     YGMKVRTRLDAATYRAWLKHALFVMALLLLAQYAYGEWFK"
     gene            complement(4660..4977)
                     /gene="grxD"
                     /locus_tag="VFY80_03895"
     CDS             complement(4660..4977)
                     /gene="grxD"
                     /locus_tag="VFY80_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX6007077.1"
                     /translation="MSVQDVIKDQVTGNRVVLYMKGTPDFPQCGFSANAIGILRACGV
                     EQPFTVNVLEDPEIRQGIKEYANWPTIPQLYVNGEFVGGCDIMIEMYQSGELQKMLDK
                     TTA"
     gene            complement(5041..>5667)
                     /gene="prmC"
                     /locus_tag="VFY80_03900"
     CDS             complement(5041..>5667)
                     /gene="prmC"
                     /locus_tag="VFY80_03900"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX6007078.1"
                     /translation="YITGRREFHSLEFRVTPAVLIPRPETELLVDLALERIAMGEARR
                     VLDLGTGSGCVAISIAYERPHARVLAVDASLQALSVARQNANRLGVSGVEFIAGDWFE
                     PIGAQLFDVIVANPPYVAEGDAHLAQGDLRYEPRDALVAGRDGLAAIRRIVVDGARCL
                     HRGGTLLLEHGCDQGPACRALLRASGLQAVETWRDLAGLERVSGGRSA"
BASE COUNT          966 a         1847 c         1797 g         1057 t
ORIGIN      
        1 gatcaagccc gtggtcggct tgagccagcg cgactggatg ccggtcttcg ccttgagcgc
       61 caggagatcg aagatcgcga tgccggttgc ttctgatccc gttccggacg tcgtcgggca
      121 ggcgatgtgt ggcttgagcg gtccgggcac cggtttgccg tcgccgaccg gcgcgttgac
      181 gtaagccagg aaatccgccg gatgcgtcga gtacagattg gctgccttgc acgtatccat
      241 gaccgagccg ccgcccaccg agacaaagcc gtcgaagcgt ccctcctgcg caaagcgcgc
      301 cgcctgcttg aaagacgcgt cggtgggctc gaccttcacc gcgtcgtaga tcgcgacgtc
      361 aatccccgcg gccctgagcg agctcgtgac ggtgtcgaag tgtggcagtg tccgcacccg
      421 cgggtcggtg tacacgccga cccgtgtcat cccaagcgcc ttcgcatgat cgccggtctc
      481 cttcaagcaa ccggcgccga acgtcagccg cgaagcgtcg atggtgaacg catcgtcccc
      541 gatgcccgtt tgcgtgaaat acgcgtgaca gcaacccatg cttgtctcct ttgatcaggc
      601 accggcgtgc cgggcttcct cgcggatcat gtcggcgccc ttctcggcga tcatcacgac
      661 cggcgcgtgc gtattgcccg aagtgagcct tggcatgatg gagcaatcga ccactcgaag
      721 cccggcaagc ccgtggacgc gcaggcgcgc atcgactacc gccatcgcgt cgtcccccat
      781 gcggcaggtt cccgacggat gaaatatcgt cgcaccgtga ttgcgggcga atgcgagcag
      841 cgccgcatcg tccaccgcgc cccggccagg gcggtattcc tcgtcgatat attcgctgag
      901 cgccggctgc gcggcaaggc gccgtgcgaa cttgatgcct tcgacggcgc agcggcagtc
      961 gatctcgctc gtgaggtagt tgggacgcat ggcaggcggc tcgaatggat cgcgcgagtt
     1021 gatcgcgacc gtgccgcggg attcgggccg aagctggcat accgaaaagg tgcagcccga
     1081 ccacttatgc ggctttccgc cggccatgtc agccgatagc gtggcgaagt gaaactgaac
     1141 gtcgggcgtt gtcgatgcag gcagcacgcg cgtaaagagt ccgccttgat taatgccgat
     1201 ggcgagcggg cccttccgat gacggagcca tctgagcccg atgcgcagct ttccggcgag
     1261 cgtccgcagg tcgtcgttgg ttgtgatcgg cttgcgtacc ttgtagatca ggcggtgctg
     1321 caggtgatcc tgcagatttt cgccgacgcc gggcaggtgc ttcacgaccg gtatgcccaa
     1381 atcctgcagc aacgttgcac ggcccacgcc ggaaagctgc agcagttgcg ggctctgcag
     1441 cgcgccggcg gcgaggatca cctcccgcac tgcgcgcacg ctcctgtcga cgccatgctg
     1501 ccgatacgtg acacccgttg cgcggagccc gtcgaacacg acgccggtta catgcgctcc
     1561 ggttgccacg cgcagattgc ggcgcctgag cgccgggcga agataagccg tcgccgacga
     1621 gcagcgccag ccgtctcgag tgaagagttg ataatagccg acgccttcct gacttgcgcc
     1681 gttgaagtcg tcgttgcgcg gcacacccag cgcctcggct gcgcggatga tcgcctccat
     1741 gagcgcatgc ggctcgccga tatccgagca ccacacagga ccctcgtccc cgtgccattc
     1801 gcttgcgccg cgtgtgttgc gctcgagctt gcggaagtac ggcaacacgt cattccagcc
     1861 ccagccgcga ttgccgagcg ccgcccagcg ttcgtagtct tcccgctgcc cgcgcacata
     1921 gataagcccg ttgatggacg aggatccgcc gaggccgcgc ccgcgcggcc agtacaccct
     1981 gcgctggttc atggcaggct ccggctcggt ataaaagccc cagttgtaaa ccgggtgaaa
     2041 catggtcttg ccgtaaccga tggggacgtg tatccagagc gagcggtctt cagggccagc
     2101 ctccagcagc agcaccgtat ggcgcccatc ttcggtcagc cggttcgcga gaacgcagcc
     2161 tgcggaaccc gcgcccacga ccacgtagtc gtaggcttcc gatgcgaccg cgctcatgac
     2221 cggtatcccg gcctctagaa gtaggccgcc ttgacgccgg gcggaggcga atagcggaag
     2281 gtcccgcgct tcagaatcgc ttcctttccg ccgtgctttc cgatgtgcgc aagctggtcg
     2341 gcacgagcct gccggtcggc cgcttcggga tctacgactt tgcgcagggc cgcctcgcag
     2401 tcggaaacga tttttcgaca ggagggctcc tgtccgagat cgatgcgttc gtgcggatcg
     2461 cgctcgagat cgaagagcat gggcggcagg ccgacatagt ggatgtactt gtagttgccg
     2521 cgccggatca tgtaggcacc gcagatcgcg cctgcagcgt gatactccga cagtatggtg
     2581 cgcacaggcg tcgcgccctg cgcgatacgg atcagcgagt gtccgggcaa cgccgcgtcc
     2641 tgcggatcgg gttccgcgcc cagcgcctcc acgatcgtcg gaaacgcatc caccagcgtg
     2701 accggcgtat cgcacgccgc gcccgctgga acatcgggtc cggccatgat catcgggatt
     2761 ccggccgatt cttcgtacat cgtcgacttg ccccagaggc cgcgcgtgcc gagattgtcc
     2821 ccatgatcgc tggaatagac aacgcgcgtc gaggcggcaa gcccgctctc ctcgagcgca
     2881 ttcagtatcg tgccgatgtt gtcgtcgagg aaggaaacga gtcccatgta ggcagtgatc
     2941 gcacggcgta ccatgggcgc atcgaacggg tcgtcgaagc acaggcactt gcgcatggca
     3001 tcgaggaacg gatggcgggg tcggctctcg tgctcgtaca tgtccggcca cggcacttcg
     3061 ttctcgggat aaagatcgta aaagccaggc ggtgcgatca gcgggaaatg cggcgatacg
     3121 aaccccacgt agagcgccca gggcctgtcg cggtaaagcg gcgcctcctc cttcagccag
     3181 cgcacggctt cccgagcgat gctgcggtcg taatgcgtgt actccgactc gccgcgcccc
     3241 gcttcgggtc cgagcttcgc ggatccgggc cggcgcggca actcgtcgcg gatgaggccg
     3301 aggagatcac ccacgccgtt gacgatgtgt agcggaagga tttccttgtc gaatccattg
     3361 cggcgcgcgt cggaatcgac gtagtgcagc ttgccgatcg aggtcacgcg atgcccttgc
     3421 gccatcaagc ggtgacccca ggacgcaatg cggccgtcat aggcgatcgc gttgtcccag
     3481 taacggatgc ggtgcacgta ctgccctgtc gcaaaggccg cgcgggccgg cacgcagatc
     3541 gggcaattcg tgtaggcatt cgtaaaccgc gtgccgcgcg cggcgagccg atcgagatgc
     3601 ggtgtcctga tcatcgcatg gccgtacgac ccgagcacgc gcttgttgtg ctcgtcggac
     3661 atgatgaata cgagattggc cggctccatc gcttgctcca ttgcgggagc gtatggtgac
     3721 acggttgcgc tcgctgcgac agaccatgac gacggcacgg tcgaaatgcg atctgctaca
     3781 ctccgcgcct tccatgcgca cgcctgctta caacgcccca acggtccgcg gctgatgggc
     3841 ggattcgaat cgctcaccct cgaagtcgcg gtatgggcgg cgctggtcat cgccttcgca
     3901 gggttcgtgc agggtgcact ggggctcggc ttccccatgg tcgccacacc gctcattgcc
     3961 ttcacgactg acatgcgcac cgcggtggtg tgggtgctgc ttccctgtct tgccgccgtc
     4021 gcagcgaacg tcgtgaaaac gggcgccttg cgcgaagcac tcgcccggtt ctggatgatg
     4081 ccgttctaca tgctgatcgg tgccgcgatc ggcacgcgcc ttttcatcat tgcgccgcag
     4141 tttcctttct cgctgttgct cgcggccatg atcatcctct atctcaatct cgatcggctg
     4201 ggacgtgcgc gctggaccgc gctcgagacg caccgcggct tctttgccgc cgtgttcggt
     4261 gtggcagcgg gcatgtcgga gggcatggca aacatcgccg cgccgccgct gctcatctac
     4321 tatcttgcgc tcggcctttc gcccgcgatg ctggtgcagg cgctcaacat ctgctttttc
     4381 gtgggcaagt cgacgcagtt cgccacgctc gccaccgtcg gaggcgtccc gcccgtgcag
     4441 tggatcgcaa ccttgccgct cgtcgtcgtc gcaagcgtga cggtgctcta tggcatgaag
     4501 gtacgcacgc gcctggatgc cgcgacgtat cgggcatggt tgaagcacgc actgttcgtg
     4561 atggctctcc ttcttctcgc gcaatacgca tacggcgagt ggttcaaatg agaaaggccg
     4621 gcgcggcaac cggcccggat gcgaacggcg gcgcgccgct caagcggtcg ttttatcgag
     4681 catcttctgc agctcgccgc tctgatacat ctcgatcatg atgtcgcacc cgccgacgaa
     4741 ttcaccgttg acgtagagtt gcgggatggt cggccagttc gcgtactcct tgatgccctg
     4801 ccggatctcg ggatcttcga gcacgtttac cgtaaagggc tgctcgacgc cgcacgcgcg
     4861 cagaatgccg atcgcattgg cggaaaagcc gcattgggga aagtccggcg tgcccttcat
     4921 gtacagcacc acgcggttcc cggtcacctg atccttgata acgtcctgaa cgctcatggt
     4981 ttctccaata gttgacaatt tcactcggct ttaaagtgta gcggctggcc gcccagtgcg
     5041 tcaagcgctg cggccgccgc tgacgcgttc cagcccggca agatcgcgcc acgtttcgac
     5101 ggcctgcaat cctgacgccc gcagcagtgc gcggcacgcc ggtccttggt cgcagccgtg
     5161 ctcgagcagc agcgttccgc cgcggtggag gcagcgtgcg ccatcgacca cgatgcggcg
     5221 tatggctgcg agcccgtccc gtccggccac cagcgcatcg cgcggctcat agcgaagatc
     5281 gccctgcgcg agatgagcat cgccctccgc aacatagggt ggattggcga cgatcacgtc
     5341 gaaaagctgc gctccgatcg gctcgaacca atcgcccgcg atgaattcca caccggagac
     5401 gccgaggcga ttcgcattct ggcgcgcaac ggagagcgcc tgcagtgatg cgtccaccgc
     5461 aagcacgcgg gcatgcggtc tctcgtacgc aatgctgatc gcaacgcagc cgcttcctgt
     5521 cccgagatcg agtactcggc gcgcctcacc catcgcgatc cgctcgagcg caagatcgac
     5581 cagcagttcg gtttccggtc gcgggatcag taccgccggg gtcacgcgaa attcgagact
     5641 gtggaattcg cgacgcccgg tgatata
//
LOCUS       DASURG010000227         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_141647, whole genome shotgun sequence.
ACCESSION   DASURG010000227 DASURG010000000
VERSION     DASURG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141647"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1156
                     /locus_tag="VFY80_03905"
     CDS             296..1156
                     /locus_tag="VFY80_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6007079.1"
                     /translation="MSRGSSLRGIAAMTAGAGLLTLNDAVSKYLTEHYALGQVICLRQ
                     AAAFLFMLPYAWAVTGLGALRSVNHGGQLLRGLLFVGSTAFTVTSLSLLPLSFVTIVL
                     FSSPLFVALTSAPMLGERVGLHQWSAIAAGFAGVLLIVRPAGAGFDWVMLLPVLAAFV
                     NGSRDTLTRRLSRTDSSISMLLWSGVAVMIAGLLTIPFGWKAVDLRGAGWFLLAGFFN
                     AAAHFVVIEAFRLGRAAVVAPFRYTGLLWAMLVGFIVWREVPDAWMLAGAAVVVCAGL
                     YMLRHGVSKT"
     gene            1173..1919
                     /locus_tag="VFY80_03910"
     CDS             1173..1919
                     /locus_tag="VFY80_03910"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007080.1"
                     /translation="MNIIAPWYQLGYIIPHLYTDLDAYQFYRVAPEGMMLVTTGLNLT
                     DYTLAAVEQELPTLWQRFGLLASKKVDRISLSGVPVASALGREKMREILAEGEKRTGI
                     ACDTDLEAHIAALQHFGARRIALATRWPERVNAALTRYLAEAGIEVIAIRSRARSLEE
                     NKHSSAAADHDLALDLGREVLRAAPEAQALLMPGGLWFAIHAVPMLEAEFGRPVLLNI
                     LSTTWAALHAAGERMRHRPDPRWGKVLASL"
     gene            1974..2099
                     /locus_tag="VFY80_03915"
     CDS             1974..2099
                     /locus_tag="VFY80_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007081.1"
                     /translation="MNVFPAKAGIQRRLNAWIPFFNGMTMLATTHDEVGQTSATL"
BASE COUNT          337 a          688 c          701 g          406 t
ORIGIN      
        1 gcagcggcgg cgtagaaata gaagcgctcg tcggtatcga cgtacgccag cgcgaggaag
       61 atgcccgcca cgccgcatgc ggcattggcc agcgtgaaga agtcggcgag atggaactcg
      121 cggatcatgg agaaatgccg gcggggccgg tcttgagcgg gcatcgcggt cctcatcgcc
      181 ctggaggcgt gcggaatggg ttcaggcttc ttataccgtg cgccgcgttg caggtccacc
      241 tcggctccat ccgcccggtg catccccggc ggggcgctgc cgccacggtt tacgcatgag
      301 ccgcggctct tcgctacgcg gtatcgcggc gatgacggcc ggcgcggggc tactcacgct
      361 gaacgacgcg gtatcgaagt acctcaccga gcactatgcg ctgggacagg tgatctgcct
      421 gcgtcaggcg gcggcgttcc tcttcatgct gccgtatgcc tgggcggtga cgggcctcgg
      481 cgcgctgcgc tcggtgaatc acggcggaca gctcctgcgc ggcctgcttt tcgtcggcag
      541 cacggccttt acggtgacga gcctcagtct gctgccgctc tcgttcgtca ccatcgtgct
      601 cttttcgagc ccgcttttcg tcgcgctcac ttccgcgccg atgctgggcg agcgcgtggg
      661 cctgcatcag tggagcgcca tcgccgcggg attcgctggc gtactactga tcgtgcgtcc
      721 agccggcgct ggcttcgact gggtcatgct gcttccggtc ctcgcggcgt ttgtcaacgg
      781 ctcgcgcgat acgctgacgc gccggctctc acgcaccgat tcctccatct ccatgctgct
      841 gtggtcgggc gtggcggtga tgatcgctgg gctgctcacc ataccgttcg gctggaaggc
      901 ggttgatctg cgcggggccg gctggttcct gctggccggt ttcttcaatg cggcggcgca
      961 cttcgtagtg atcgaagcgt tccgcctcgg ccgcgccgcg gtggtcgcgc cgtttcgcta
     1021 taccgggctc ctgtgggcga tgctcgtggg attcatcgtc tggcgtgaag tgcccgacgc
     1081 ctggatgctg gcgggcgccg cagtggtcgt atgcgcgggg ctctacatgc tgcgccatgg
     1141 cgtatcgaaa acgtgagatt cggagacaaa tcgtgaacat catcgcaccg tggtaccaac
     1201 tgggatacat catcccgcat ctgtataccg atctcgatgc gtaccagttc taccgcgtcg
     1261 cgcctgaagg catgatgctc gtgacgaccg ggctcaatct caccgactac acgctcgcgg
     1321 cagtcgaaca ggaactgccc acgctgtggc agcgcttcgg actcctcgcg tccaagaagg
     1381 tggatcgcat ttcgctttcg ggtgtcccgg tcgcatccgc cctcggccgc gaaaagatgc
     1441 gggagatcct ggccgaaggc gaaaaacgca ccggaatcgc gtgcgacacc gatctcgaag
     1501 cgcacatcgc ggcgctgcag cactttggcg cgcgccggat cgcgctcgcg acgcgctggc
     1561 cggagcgggt caacgcggcg ctcacgcgct atctcgcaga ggccgggatc gaagtgatcg
     1621 cgattcgctc gcgggcgcgc tcgctggaag aaaacaagca ttcgagcgcc gccgccgatc
     1681 acgatctcgc gctcgatctc ggccgcgagg tgctgcgcgc cgcacccgag gcgcaggcgc
     1741 ttctcatgcc gggggggctt tggttcgcca tccacgccgt gccgatgctc gaagcggaat
     1801 tcggacgccc cgtgctgctc aacatccttt cgacgacgtg ggcggcgctg cacgcggcgg
     1861 gtgagcgcat gcggcaccgg cccgatccgc gctggggcaa agtgctcgcg agcctctaac
     1921 gttgccttca agaacagacg atcttcccgc ggaataaacg tctccccggc gcaatgaacg
     1981 tcttcccggc gaaagccggg atccagcgaa gacttaatgc ctggattccg tttttcaacg
     2041 gaatgacgat gctggcgaca acgcacgacg aagtgggcca gacgtccgcc actctgtgac
     2101 agtcttgcca caagatgaac gtctgcttag cg
//
LOCUS       DASURG010000228         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1060988, whole genome shotgun sequence.
ACCESSION   DASURG010000228 DASURG010000000
VERSION     DASURG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060988"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(13..200)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            593..1702
                     /locus_tag="VFY80_03920"
     CDS             593..1702
                     /locus_tag="VFY80_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6007082.1"
                     /translation="MDGKPGAGNAITDVAGVEVGQVTLIRGEGALRRGEGPVRTGVTA
                     IIPRGKSSTNPVFAGWATSNAAGEMTGTTWLEERGYFDGPIMITNTHSVGVVRDAVVG
                     WIVDRKWPGSWYTPVVAETYDGYLNDTDGMHVTKEHALEALAKAQSGPVEEGNVGGGT
                     GMVCFGFKGGIGTSSRLVTTAIGTYTVGVLVQCNFGLMPTLRIGGIKVGEALKDRYAT
                     CTTQPPSPYPGIRTCGPDPKGEKPGDGSMIVVIATDAPLLPHQLKRLAKRPAHAMGRL
                     GDTGRETSGDIFLAFSTANSQIVSEQREKMALDAYPNGMLNPLFEGAVQAVEESILNA
                     LVAARTMTGRDGHRVYGLPHDEVRALLKKHGVLVQ"
     gene            1699..>2432
                     /locus_tag="VFY80_03925"
     CDS             1699..>2432
                     /locus_tag="VFY80_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX6007083.1"
                     /translation="MTATLQRRSWVPEPSEELVQRLAGAAAVADATLVEGRITSLVRE
                     NREIHEDLCINLNPATNVMNPKAEALLASGLGTRVSLGYPGDKYEMGLEAVEQIEVIA
                     AELAAEIFGARFAEIRVQSGAMANIYAFMATCSPGDRIIVPPDAIGGHVTHHQAGAAG
                     LYRLEIHEAPVDAGGYTVDLDGLRALATKIRPRLITLGGSLNLFAHPVREVRGIADEV
                     GAAVLFDAAHLSGMIAGGVWPNPLAEG"
BASE COUNT          449 a          749 c          830 g          404 t
ORIGIN      
        1 caccccggcc agcgaaagac gacggcgccg agcaggtctc ctggctcgcg gcttggctcc
       61 ggcgccacct tcccaagccc gtcaggctca gtggtctttc ggcgcggata accgcttaca
      121 gttgcggggg cagcccggga ctttcacccg gttccctttc gatcccacgg ttcgcgggaa
      181 ctcgacgcga agcgaaggat gcggacagcc gcgcccagcg tcaatcctta agctgcggtt
      241 atggaggcca tgaacggaat taacttcgac cgcggggcgc tactgaccca caatcgcatg
      301 ccagctcagg gagccagcca tgcttgcgga agaatatctc tcgccaagac gccaaaacgc
      361 cagggtagag ccggaagatc tggtcgtccc cgcgaaagcg gggacccagt cttgcaacct
      421 ggattcccgc tttcgcggga atgacaggat ccgatctcat tcggattctt tttggcgttt
      481 tggcgttttg gcgagagaca tcttccagct cctggtaatt gcctcgagct gcatccttgc
      541 gagcttgcct gcgtctgcag cgacgccgag ggcgagagac atagggatac cgttggatgg
      601 aaagccgggt gcggggaatg cgatcacgga tgtggcgggc gtcgaagtcg ggcaggtcac
      661 gctgatcaga ggcgaaggtg cgctcaggcg cggcgaaggg ccggtgcgta cgggagtcac
      721 cgcgatcatt ccgcgcggca agagttcgac gaatcccgtg ttcgccggat gggctacgag
      781 caacgccgca ggcgagatga ccggcacgac gtggctggag gagcgcggct acttcgacgg
      841 gcccatcatg atcacgaata cccacagcgt cggcgtcgtc cgcgacgcgg tcgtcggctg
      901 gatcgtggat cgcaagtggc cggggagctg gtacacgccg gtcgtggcgg aaacctacga
      961 cggctatctc aacgataccg acggcatgca cgtaacgaag gagcacgcgc tcgaagcact
     1021 ggcgaaggcg caaagcgggc ctgtcgaaga gggcaacgtc ggcggcggca ccggcatggt
     1081 ctgcttcggc ttcaaaggcg gcatcggcac ttcgtcgcgc ctcgtcacga cggcgatcgg
     1141 cacatacacg gtcggggtgc tggttcaatg caacttcggt ctgatgccga cactgcgcat
     1201 cggcggtatc aaagtgggcg aggcgctcaa ggaccgctac gcaacctgca cgactcaacc
     1261 gccgtccccg tatcccggta tccggacctg tgggcccgat ccgaagggcg agaagccggg
     1321 cgacggctcg atgatcgtcg tcatcgccac ggatgcaccg ctcctgccgc atcagctcaa
     1381 gcgcctggcg aagcggcccg ctcacgcgat ggggcggctc ggcgacaccg ggcgcgaaac
     1441 ttccggggac atcttcctcg cgttctcgac cgcaaactcg cagatcgtga gtgagcagcg
     1501 ggagaagatg gcgctcgacg cttatccgaa cggcatgctg aatccgctct tcgagggcgc
     1561 cgtgcaggcg gtcgaggaat cgatcctcaa cgcgctggtg gcagccagga ccatgactgg
     1621 ccgtgacggc catcgcgtct acggcttgcc gcatgacgaa gtgcgcgcgc tcctgaagaa
     1681 gcacggagtg ctcgtgcagt gactgctacg ctgcagcgac gatcgtgggt gcccgagccg
     1741 agcgaagagc tggtgcagcg gctcgccggc gctgcggcgg tggccgatgc aacgctcgtc
     1801 gaaggccgca tcaccagcct cgtgcgggag aatcgcgaga ttcacgaaga tctctgcatc
     1861 aatctcaacc cggcgaccaa cgtcatgaac ccgaaggccg aggcgctgct ggcgagcggt
     1921 ctcggcactc gcgtgtcgct cggctatccg ggcgacaagt acgagatggg actggaagcc
     1981 gtcgagcaga tcgaagtgat cgccgcagag ctcgcggcgg aaatcttcgg cgcgcgtttt
     2041 gccgagattc gcgtgcagtc gggcgcgatg gcgaatatct atgcattcat ggcgacgtgc
     2101 tcgcccggcg accgcatcat cgtgccgccg gacgcgatcg gcgggcacgt gacgcaccat
     2161 caggccggcg cagcgggcct gtaccgactg gagattcacg aagcgccggt cgatgccggc
     2221 ggctacacgg tcgacctcga cggcctgcgg gcgttggcga cgaagatccg gccgcgactc
     2281 atcacgctgg gcggcagtct caatctgttt gcgcatcccg tgcgcgaggt gcgcgggatt
     2341 gccgatgaag tcggcgcggc ggtgttgttc gatgctgcgc atctgtcagg aatgattgcc
     2401 ggcggcgtgt ggccgaaccc gctcgccgag gg
//
LOCUS       DASURG010000229         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_960538, whole genome shotgun sequence.
ACCESSION   DASURG010000229 DASURG010000000
VERSION     DASURG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960538"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFY80_03930"
     CDS             complement(<1..563)
                     /locus_tag="VFY80_03930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX6007084.1"
                     /translation="MPGALQDYEALLWMLAVVPAFLLAYYKAWRGAAVALAAAMAVVS
                     VTYAVTQILVRPMPELMLPVLVILIAISLGVGALAERLHRDAGHESAGGFTDPATGLP
                     NRAHADLHLESEFKSSAGRSFAVVLFDVDNFKGFNSRHGAIAGDEVLRMVAEVLKKMT
                     RRMNLSARYAEDEFLAVLSGADEEGAIA"
     gene            complement(733..1257)
                     /locus_tag="VFY80_03935"
     CDS             complement(733..1257)
                     /locus_tag="VFY80_03935"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HEX6007085.1"
                     /translation="MENLRYPLGRFDPKQPLPPGGHAVLIRSLASTPIHMRRAVAGLT
                     EEQVATPYREGGWSVRQLVHHVPDSHMNAYVRMKWGLTESTPLIKTYDEKAWSELPDA
                     RTAPIDVSLDLLEAVHRRWDMLLEAMTDQDFARTVRHPDWGEISLTTLLQLYEWHGRH
                     HVAHITGLRERRQW"
     gene            1356..1505
                     /locus_tag="VFY80_03940"
     CDS             1356..1505
                     /locus_tag="VFY80_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX6007086.1"
                     /translation="MLWTIFIILLVLWLLGLVSGTTLGGFIHVLLVIAIVVILIRLIS
                     GRRPV"
     gene            1724..>2292
                     /locus_tag="VFY80_03945"
     CDS             1724..>2292
                     /locus_tag="VFY80_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6007087.1"
                     /translation="MNKHSHGRPAIGTNGGLDFDEAPFVVIWETTQACDLACKHCRAE
                     ACPDRHPEELTTAEAKALMKRVRDFGKVVFVLSGGDLLKRDDSIELVKYGADLGLRMG
                     VTPATTPLCSPEKIRELKDAGITRLAVSLDGSCPEIHDEFRRVPGSFDWGMKILRECR
                     ELGISTQVNTVIGSHNVHDFDKLCALMAEL"
BASE COUNT          464 a          710 c          688 g          430 t
ORIGIN      
        1 gcaatcgcgc cttcttcgtc ggcacccgac agcacggcca ggaattcgtc ctccgcataa
       61 cgcgccgaca ggttcatccg ccgcgtcatc ttcttcagaa cttcggcgac catgcgcaat
      121 acttcatcgc ccgcaatggc accatgccgt gagttgaagc ccttgaagtt gtcgacgtcg
      181 aatagcacga ccgcaaagct ccgacccgcc gacgacttga attcactttc caaatgaaga
      241 tcggcatgcg cgcgattggg caagccggtg gcaggatcgg tgaaaccgcc ggcgctttcg
      301 tgtccggcgt cgcgatgcag acgttcggcg agcgcgccga caccgaggga tatcgcaatg
      361 aggatgacca gcaccggcaa catcagctcg ggcatgggac gcacgaggat ctgtgtgacc
      421 gcatacgtga ccgaaaccac ggccatcgct gccgccaacg cgaccgcggc gcctcgccag
      481 gctttgtagt acgccagcag gaacgcaggc acgacggcca gcatccacag cagcgcttcg
      541 tagtcctgca gcgcgcccgg cacccaaaac gcacccgcga ccggaatcgc gagcgccgca
      601 agggaaataa tcagcgcact tacaggcggg tgatggccag ccgacattac gcgtgtgtta
      661 aatgatcggg agagctgcgg gtgcaacgta ttgaaatatc agcaattatg cagcacccgg
      721 caaggacgcg cgctaccact gccgccgctc ccgcagcccc gtgatatggg cgacgtggtg
      781 ccggccatgc cactcgtaca gctgcagcag cgtggtgagc gagatttcac cccaatccgg
      841 atgccggacc gtgcgcgcga aatcctgatc ggtcatggcc tcgaggagca tgtcccagcg
      901 gcgatgcacg gcctccagca agtcgagcga gacatcgatg ggcgcagtgc gcgcatcggg
      961 cagctcggac cacgcttttt catcgtaggt cttgatcaac ggcgttgact cggtaagtcc
     1021 ccatttcata cggacatacg cgttcatgtg actgtccggc acatggtgga cgagctgtcg
     1081 aacgctccag ccgccctcac gatacggcgt ggcaacctgt tcctcagtca gcccggccac
     1141 ggcccgtcgc atatgaatcg gggtgctggc cagcgagcga atgagcaccg cgtgcccacc
     1201 cgggggcaac ggctgcttcg gatcaaaccg tccaagagga taacgcaaat tttccatgtg
     1261 gctcctgatg ttctggtgag aatgcagccc tgtaccgaat cggcccgaac cttgcccttc
     1321 tacctgcccg tccgacgact aacagggagg ttgggatgct ttggacaatt tttatcatcc
     1381 tgctcgtgtt gtggctgctg gggttggtat ccggcacgac cctgggcgga ttcattcatg
     1441 ttctgctggt gattgcgatt gtggtgattc tgatccggct catttccggc agaaggcccg
     1501 tttaacggca atacgtcgca acgacacgac gtgaccgaac ccgtgacggg gacggcgcga
     1561 acggcgtaaa ggcgtgagcg gcataacggc tgctccgcct ttatgcgttt tccggcgccg
     1621 gcagcccagt gcgggccgcc tgcgcccgcg cacccggatc ggtttacgat ttatcctcct
     1681 tttccctaca aacaatcatt ctcgcctggc gtacgttccg gccatgaaca agcattctca
     1741 tggccgcccg gccattggta cgaacggggg cctcgatttc gacgaagcac cctttgttgt
     1801 tatctgggaa actacccagg cctgcgacct ggcgtgcaaa cactgccggg cagaagcctg
     1861 tcccgaccgg catccggaag agctgaccac cgcggaagcc aaggcgctga tgaagcgcgt
     1921 gcgcgacttc ggcaaggtcg tctttgtcct cagcggcggc gatctgctca agcgtgatga
     1981 ttcgatcgag ctggtaaaat acggcgccga tctgggttta cgcatgggcg taacacccgc
     2041 gaccacacca ttgtgcagtc cggagaaaat ccgcgagctg aaagacgcgg gcattacccg
     2101 gctggcggtc agtctggatg gctcgtgccc ggagatccac gatgagttca gacgtgtgcc
     2161 gggatccttc gattggggca tgaagatcct gcgggaatgt cgcgagctcg gcatctcgac
     2221 gcaggtgaac acggtgatcg gcagccacaa cgtgcacgac ttcgacaagc tgtgcgcgct
     2281 catggccgag ct
//
LOCUS       DASURG010000230         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_551335, whole genome shotgun sequence.
ACCESSION   DASURG010000230 DASURG010000000
VERSION     DASURG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551335"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1499
                     /locus_tag="VFY80_03950"
     CDS             153..1499
                     /locus_tag="VFY80_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX6007088.1"
                     /translation="MNDPKDAFKLTGHGPEWKPAHLAPSEARAATAWVEARVDRSSLT
                     GKDRVEDVRDLMWQLERDGEIVVHRIRDEHEPVETKTLYGWTKRIPTARLWHHKSCGQ
                     CGNIPGYPTSLLWLMNELGTHYLDETDQTSCTAWNYHGSGIGNLESLACVFLRNFHQA
                     YVSGRAEGHADGFYFPLVHCGTSFGNYKEVRAFLLRSADLREKVKKVLAKLGRLVDGK
                     LLIPEEVVHYSEWLHVMRSRIRALQKIDVSHLRAVIHPACHVYKMVPEDVIYDDKVLD
                     GNRVAVSTGIIQELGAEVVDYSTWYDCCGFGFRHIISEREFTRSFAMDRKVKVAVEEA
                     RADVMIGHDTGCITTLDKNQWIGKAAGKTAEIPIVADCQFAALACGADPYKIAQLHWH
                     ASPVEKLLSQMGIDWEKKKAEFEAYLQEVQAGRGEHLYDPRRAVTSGPGFRKPARV"
     gene            1503..>2021
                     /locus_tag="VFY80_03955"
     CDS             1503..>2021
                     /locus_tag="VFY80_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6007089.1"
                     /translation="MTVLVVGGGPAGLAAAHALAGVGEAVTLVDKADRLGGAPILSGY
                     AKLIPSGVWARDAIGGMVRRVEEQPRATIEKHTTVARFEGEPGRFTAHLSSGKSVECK
                     AAILCTGFTHFDSVNKPEWGFGTYPDVVTTTQVEQMISSGRGVRCPSDGRKPERVAIL
                     LCVGSRDRQIGRE"
BASE COUNT          406 a          613 c          661 g          341 t
ORIGIN      
        1 tgaccggcgt gctgaagcag ctggagacga tcgacgagaa tctgttcgat gtcatctcgg
       61 acatcatgga cgagaagcgc gaggactatg aggactggct cgagtcgcag gaggaaggcg
      121 attcctgagt cgggccaacg aggagatcga cgatgaatga tccgaaagac gcattcaagc
      181 tgaccggcca cggccccgag tggaagcccg cgcatctcgc accgagtgaa gcgcgcgcgg
      241 cgaccgcctg ggtggaggcg cgggttgacc ggtcgagcct gaccgggaaa gaccgcgtcg
      301 aagacgtgcg cgatctcatg tggcaactgg agcgcgacgg cgagatcgtc gtgcaccgta
      361 ttcgcgacga gcacgagccg gtcgagacca aaacccttta cggctggacg aagcgcattc
      421 cgactgcacg cctctggcac cacaagtcgt gcgggcaatg cggaaacatc ccggggtatc
      481 ccacttcgct cctgtggctc atgaacgagc tcggcacgca ttacctcgac gagacggacc
      541 agacctcttg caccgcctgg aactaccacg gatcgggaat cggcaacctc gagagcctcg
      601 cttgcgtctt cctgagaaac ttccatcagg cctacgtctc gggtcgtgcc gaagggcatg
      661 ccgacggatt ttactttccg ctcgtccatt gcggcacctc gttcggcaac tacaaggaag
      721 tgcgcgcgtt cctgttgcgc tcggcggacc tgcgcgagaa agtgaagaaa gtgctcgcca
      781 agctggggcg gctggtggac ggaaagctcc tcatcccgga agaagtggtg cattactcgg
      841 aatggctgca tgtcatgcgg agccgcatcc gcgcgctgca gaagatcgac gtgagccatc
      901 tgcgcgcggt gatacatcct gcatgtcacg tctacaagat ggttccggag gacgtgatct
      961 acgacgacaa ggtgctcgac ggcaaccgcg tcgccgtgtc gaccggcatc atccaggaac
     1021 tcggcgcgga agtggtggac tactccacct ggtatgactg ctgcggtttc ggtttccggc
     1081 acatcatctc cgagcgcgaa ttcacccgct cgttcgcgat ggatcgcaaa gtgaaggtgg
     1141 cggtcgagga agcgcgtgca gacgtgatga tcggccacga cacgggctgc atcacaacgc
     1201 tcgacaagaa ccagtggatc ggaaaagccg cgggcaagac ggcggaaata ccgatcgtgg
     1261 ccgactgcca gtttgcggcc cttgcctgcg gcgccgatcc ctacaagatt gcgcagctcc
     1321 attggcacgc ttcgcccgtc gaaaaactgc tctcgcagat gggcatcgac tgggagaaga
     1381 aaaaagcgga gttcgaagcg tatctccagg aagttcaggc cggccgcggc gagcatctct
     1441 acgacccgcg ccgcgcggtg acctcggggc ccggctttcg caagccggca cgggtgtagg
     1501 ccatgaccgt gctcgtcgtc ggcggcggac ccgccggcct tgccgcagcg catgcactcg
     1561 ctggcgtcgg cgaggcagtg acgctcgtgg acaaagccga tcgtctgggc ggcgcgccca
     1621 tactgtccgg ttatgcgaaa ctcattcctt caggcgtttg ggcgcgtgat gccatcggcg
     1681 gcatggtgcg cagggttgaa gagcagcccc gcgcgacgat cgagaaacac accacggtag
     1741 cgcgcttcga aggcgagccg gggcgcttca ccgcgcatct gtccagtggc aagagcgtcg
     1801 agtgcaaagc ggcgatcctc tgcaccggat tcacccattt cgattccgtc aacaagccgg
     1861 aatggggctt cggcacctac ccggacgtcg tgacgacgac ccaggtggag cagatgatct
     1921 cctccgggag gggtgtgcgt tgcccatcgg acggccgcaa gcccgagcgc gtggcgatcc
     1981 tgctctgcgt cggctcgcga gatcgccaga tcggccgcga g
//
LOCUS       DASURG010000231         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_551400, whole genome shotgun sequence.
ACCESSION   DASURG010000231 DASURG010000000
VERSION     DASURG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551400"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1187)
                     /gene="aroB"
                     /locus_tag="VFY80_03960"
     CDS             complement(111..1187)
                     /gene="aroB"
                     /locus_tag="VFY80_03960"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX6007090.1"
                     /translation="MSALTVELGERSYPIYVGAGLLDRPQLIRAHLPQPRVAVVTNAT
                     VAPLYLEQVEKSLATQGIDVVRIIVPDGERYKSWETVNSIFDALIAAHCERSTAILAL
                     GGGVVGDLAGFAAAIYQRGVPYVQLPTTLLAQVDSAVGGKTAINHPRGKNMIGAFYQP
                     RAVIADTDTLVSLPDRELAAGIAEVVKYGLIRDAVFFLWLERNMERLVTREPEALAHA
                     IVTSCSNKATIVALDERETGVRALLNYGHTFGHAIEAGTGFGTWLHGEAVACGMVLAA
                     RLSQRLGLITDSDVSRVIDLLSRARLPVARPDLGFDRYLQLMGHDKKVEGGRSRFVLL
                     RGIGDAFVTADVAQSALREALDYA"
     gene            complement(1184..1783)
                     /locus_tag="VFY80_03965"
     CDS             complement(1184..1783)
                     /locus_tag="VFY80_03965"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX6007091.1"
                     /translation="MSQSTGDARARNIPGSIYLVGLMGAGKTSVGRILARRLRKPFYD
                     MDHEIERATGVRIPVIFEIEGESGFRARESKVLAELVGKGDIVLATGGGAVLAEQNRR
                     LLNEHGTVVYLRAAPRDLWLRTRHDKNRPLLQTADPLAKLEELFAQRDPLYREAAHLI
                     MDTGCQSVGLLAHRLESRLLRLKGGPGSLAYPGQEASQR"
     gene            complement(1883..>2392)
                     /locus_tag="VFY80_03970"
     CDS             complement(1883..>2392)
                     /locus_tag="VFY80_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX6007092.1"
                     /translation="FLNLELSALQADGKGKIISSPRVITADKNEATIEQGTEIPYQQA
                     TSSGATAVQFKKATLSLKVKPQITPDNNVIMSLRVNKDSVGQVTAGGPAIDTKQVSTE
                     VLVENGGTVVIGGIYTQEERSTTNKVPVLGDLPYVGFLFKNNTKTDDRRELLIFITPR
                     ILKETLTQR"
BASE COUNT          460 a          746 c          730 g          456 t
ORIGIN      
        1 atgcggtcgc ggtcgcgctg gaactccgtg cgtccgcgtg gaggcgactc gggatattgc
       61 cgaccggcgg aacgcgcagg atcggcggca tacggagccc gctcggacat tcaggcgtag
      121 tcgagcgcct cgcgcagcgc cgactgggcc acatcggccg tgacgaaagc gtccccgatg
      181 ccgcgaagca ggacgaagcg tgacctgcca ccttcgacct tcttgtcgtg ccccatcagt
      241 tgcagataac ggtcgaagcc gaggtcggga cgcgcaacgg gcaatcgcgc gcgggaaagc
      301 aaatcgatga ctcgtgatac gtcgctgtcc gtaataagcc ccagccgctg cgagaggcgg
      361 gccgcgagca ccataccgca cgcgaccgct tcgccatgaa gccacgtgcc aaaacccgtg
      421 ccggcttcga tcgcatgccc gaaggtatgt ccgtaattga gcagggcgcg cactccggtt
      481 tcgcgctcgt caagcgcaac gatggtcgcc ttgttgctgc aggaagtgac gatggcgtgt
      541 gccagcgcct ccggttcccg ggttacgaga cgctccatgt tacgttccag ccaaagaaaa
      601 aaaacagcat cgcgaatgag gccgtacttc acgacttccg ctatgccggc ggcaagctcc
      661 cggtcgggaa ggctcacgag ggtatcggta tcagcgatga ccgcgcgcgg ctggtagaac
      721 gccccgatca tgttctttcc acggggatgg ttgatcgccg ttttgccgcc caccgcggag
      781 tcgacctgag cgagcaacgt ggttggaagc tggacgtaag gcacgccgcg ctggtagatg
      841 gcagccgcaa acccggcgag atcgccgacg acgcctccgc cgagagcgag gattgcagtg
      901 ctgcgctcac agtgtgccgc aatgagcgcg tcgaagatag aattaacggt ttcccacgac
      961 ttgtagcgct cgccgtcggg cacgatgatc ctcacaacat cgatgccttg cgtggcgagg
     1021 ctcttttcca cctgctccaa gtaaagaggg gcgaccgtcg cgtttgttac aaccgcaact
     1081 cgcggctgag gcagatgcgc gcggatcagc tgtgggcggt cgaggagtcc ggctccgacg
     1141 tatatcgggt aactgcgctc gccgagctcg acggtcaacg cgctcatcgc tgcgacgcct
     1201 cctggcccgg gtacgcgagc gatcccggcc cgccctttaa ccgtagaagc ctcgattcca
     1261 gtcggtgagc cagcaagccg acactctggc agccggtatc catgatgaga tgggccgctt
     1321 cgcgatacag cgggtcgcgc tgcgcgaaaa gctcttcgag cttcgccagg ggatcagcgg
     1381 tctgcaacag gggacgattc ttatcgtggc gggtacgcag ccacaggtcc cggggcgcgg
     1441 cgcgcaggta caccaccgtg ccatgctcgt tcaagaggcg gcgattctgc tccgccagaa
     1501 ccgcaccgcc accggttgca aggacgatat cgcccttgcc gacaagttcc gcaagcacct
     1561 tggactcgcg ggcccgaaaa ccggattcgc cttcaatctc gaagatcacg ggaatcctga
     1621 ccccggtggc gcgctcgatt tcgtggtcca tatcataaaa cggcttgcgc agccgccgcg
     1681 ccagtatccg gccgacggac gtctttccag cgcccatgag gcccaccaga taaatgctgc
     1741 ccggaatgtt gcgcgctcgc gcgtccccgg ttgattggct cattcgtcga ttctagcgga
     1801 atcgcgctcg gaagacctcg cgagcgaact gcacaaacga aaagagcggc caaggccgct
     1861 ctttagacga tcggtgccgc gcttatcgtt gagtgagcgt ttccttcagt atgcgcggcg
     1921 tgatgaagat caacaactcc cggcggtcat cggtcttagt gttgttcttg aagagaaagc
     1981 cgacgtacgg aaggtcgccc aataccggca ccttgtttgt cgtggagcgc tcctcctggg
     2041 tataaatacc gccaatcaca accgtcccgc cgttctcaac caatacttcg gtcgaaacct
     2101 gcttggtgtc gatcgccggg ccacctgcag ttacttgccc cacgctgtcc ttgtttaccc
     2161 ggaggctcat gatgacgttg ttgtccggag tgatttgcgg cttcaccttg agtgacaaag
     2221 tcgccttctt gaactgcaca gccgtcgccc cgctagaggt ggcctgctga tacgggatct
     2281 cggtaccctg ttcgatggtg gcttcgtttt tgtccgccgt gatgacacgc gggctcgaaa
     2341 tgatcttccc tttcccgtcc gcctgcaggg cggaaagctc gagattcaga aa
//
LOCUS       DASURG010000232         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_40998, whole genome shotgun sequence.
ACCESSION   DASURG010000232 DASURG010000000
VERSION     DASURG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40998"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFY80_03975"
     CDS             complement(<1..899)
                     /locus_tag="VFY80_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6007093.1"
                     /translation="MDLLMEHPLPMWVVDTALLRFLDVNPAAERTYGYTREQFLHMRP
                     GDLLPADQRSPQPLLPDAEVHARARALHVTAAGRMLEVEQYAQRITYRNDAALLITVV
                     DVSGLRTARCPEEGMLRSVSDQLPDVVFVKDREGRYVFVNQASAALLGRPAGEIIGRL
                     DSDFSSPEATAGFRAADAEAMAASEPTIVHEWVRYGEREAFMQTRKAAWRDEQGTVRG
                     VIGIARDLTELKRAERELHDIQQRYRIAFEHAPIGMALAAVPSGRWLYVNEAMCRITG
                     YSADELSQLGFHDLTHPDDLPATL"
     gene            complement(980..1900)
                     /locus_tag="VFY80_03980"
     CDS             complement(980..1900)
                     /locus_tag="VFY80_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6007094.1"
                     /translation="MKEVFALIKETIQDFSEDRCARMAAALSYYTVFSLPALLILVLS
                     IVGFFVDPGAAQGRFMDQLAGLIGPRGGEAMRTMIEQANQPRGGIMAVVGIIALILGA
                     TGAFAQLQDALNTAWEVKPDPAAGGVKQFVAKRFLSFGMILVIAFMLLVSFVASAAIA
                     AFGDLLDRYLGSAGEFIGQVTQIVLGLLVAWALFALMFKVLPDARIKWRDVAVGAFVT
                     AVLFTVGRFLIGLYLGKSASADAFGAAAALAIVFVWIYYAANILFLGAEFTQVWVRHQ
                     GRRLEPEPGAMKVPATRPADALARRTQTPL"
     gene            2004..>3566
                     /locus_tag="VFY80_03985"
     CDS             2004..>3566
                     /locus_tag="VFY80_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6007095.1"
                     /translation="MPTDDSDTQQEADRQRFLAEASHLLSSSLDYTETLQRLAQLAVP
                     RVADWCAIYITNAEGRLEPVEVAHPDAAQRQLAMDMRRRHPPDPASDYGAYHVLRTGR
                     SEMIREVTNEMIRASNLPADQIEVLEQLGFRSAMTVPLRARGRTFGVIQFVSSGSGRL
                     FNHEDLAFAEDFAARAALATDNARLFQLSQRRQREEAALRAATEAVTASHSVDDIIRQ
                     IATSAIAATEADGSFVERIRGEDVEVVAVGGRLHPPLGERESFEGSVAQRVVESNQPE
                     LIRQLSEHPQRLPGVLGGMEAEARALAVPLVDGGEAIGALVLLRDARREPFTKDEAER
                     AHSFGNLAALAFRKVHLLEDTERRREELEQVIKSRSRLMRGFTHDLKNPIGAADGHAA
                     LLEDGLLGQLSDEQVHSVRRIRASLDNALKLIDALNEFARAETGRIEIRQAPVQLIEI
                     ARELGEQYRGIAAQKGLAVDLELHKVPIITSDDDRIRQILGNLLSNAVKFTNTEGRIT
                     MRTDVRPNEHGEC"
BASE COUNT          695 a         1157 c         1120 g          594 t
ORIGIN      
        1 agcgtagcgg gcagatcatc cggatgcgtc agatcatgaa agcccagctg gctcagctca
       61 tccgcactgt agcccgtgat gcggcacatc gcttcattca cgtacagcca gcggcccgac
      121 ggcacggcag ccagcgccat gccgatgggc gcgtgctcga acgcaatccg atagcgctgc
      181 tgtatatcgt gcagctcgcg ctccgcacgc ttcagctccg tcagatcacg ggcaatcccg
      241 atcacaccac ggaccgtgcc ctgctcatcg cgccacgccg ccttgcgcgt ttgcatgaac
      301 gcctcgcgct cgccataacg tacccactcg tgcacgatgg tcggctcgct cgccgccatc
      361 gcctcggcat ccgcggcccg gaagcccgcc gttgcttccg gactcgagaa atcactgtcc
      421 agccggccga tgatttcgcc cgccggacga ccgagcaatg cggccgatgc ctgattcacg
      481 aagacgtagc gcccctcgcg atccttgacg aagacgacgt cgggtagctg atccgatacc
      541 gagcgcagca tgccttcttc cggacagcgc gcggtgcgca gccccgacac gtcgacgacc
      601 gtgatcagaa gggccgcgtc attccgatac gtgatgcgct gcgcgtactg ctcgacctcg
      661 agcatgcggc cggccgcagt cacatgcagc gcgcgcgcac gcgcgtggac ttcggcatcc
      721 ggcagtaatg gctggggcga tcgctgatcg gccggcagca aatcacccgg cctcatgtgc
      781 aggaactgct cgcgtgtgta gccatacgtg cgctcggccg ccggattgac gtccaggaag
      841 cgcaataaag cggtgtcgac cacccacatg ggcagtgggt gctccatcag tagatccatt
      901 cccaccatta ggaattttta ttttcgagga ggttggacta atttaacacc ggatggttaa
      961 catcttgtag ggattatcct tacaatggtg tctgtgtacg cctcgcgagc gcgtcggccg
     1021 gccgcgtggc cggcactttc atggctcccg gctcgggctc gagccggcgg ccctggtgcc
     1081 ggacccatac ctgcgtaaac tccgcgccca ggaaaaggat gttggccgcg tagtagatcc
     1141 agacgaagac aatggcgagt gcggccgcgg cgccgaaggc gtcggccgag gcgctcttgc
     1201 cgagatacag tccaatgagg aagcggccga cggtgaagag caccgccgta acgaaggcac
     1261 cgaccgcaac gtccctccat ttgatcctgg cgtcgggcag cactttgaac atgagggcaa
     1321 aaagcgccca ggccaccagc aggccgagta cgatctgcgt gacctggccg atgaattcgc
     1381 ccgcgctgcc gagatatcga tcgagcaggt cgccgaaggc cgcgatcgcg gccgacgcca
     1441 caaaggaaac cagcagcatg aaggcgatga ccaggatcat gccgaaggaa aggaaacgct
     1501 tggcgacgaa ctgcttcacg ccgccggcgg ccgggtcggg ttttacttcc cacgccgtgt
     1561 tcaacgcatc ctgcagctgg gcgaaggcgc cggtagcgcc caggatgagc gcaatgatgc
     1621 cgaccacggc catgatgccg cccctgggct gattggcctg ctctatcatg gtgcgcatgg
     1681 cttcaccgcc gcgcgggccg atgaggccgg ccagctgatc catgaagcgg ccctgcgccg
     1741 cgcccggatc gacaaagaag cctacgatgg agagcaccag gatgagcagc gcgggcagcg
     1801 aaaagaccgt gtagtacgag agcgccgccg ccatgcgcgc gcacctgtcc tcgctgaagt
     1861 cctggatcgt ctccttgatg agcgcaaaga cttccttcat gaagcagcgg ctattgcagg
     1921 aaacgttctc tctgcatgac gcagcgggag caaatcgcgg ttgcctgaac ggcacgcggg
     1981 gtgcataaag agatcgagcc tgtatgccga ctgacgattc cgacacgcag caggaagccg
     2041 accgccagcg gtttctggcc gaagcttctc atctgctcag ctcatccctc gattataccg
     2101 agacgctcca gcggctggcg caactggccg tgcccagggt ggccgactgg tgcgccatct
     2161 acatcaccaa tgcggaaggc cggctcgaac cggtggaggt cgcgcatccc gatgccgcgc
     2221 aacgccagct cgcgatggat atgcgacgtc gtcatccccc cgacccggca tccgattacg
     2281 gtgcctatca cgtgctgcgc accgggcgtt ccgaaatgat tcgtgaagtc acgaatgaga
     2341 tgattcgcgc atccaatctg cccgccgacc agatcgaagt gctcgagcag ctcggcttcc
     2401 gctcggccat gaccgtgccg ctgcgtgcgc gcggccgcac cttcggcgtc attcaattcg
     2461 tcagctcggg cagcggccgt ctcttcaatc atgaagacct ggcatttgcc gaagattttg
     2521 ccgcgcgcgc cgcgctcgct accgacaatg cgcgcctgtt tcagctttca cagcgtcgcc
     2581 agcgcgagga agcggcgttg cgtgccgcca cggaagcggt cacggcatca cacagcgttg
     2641 acgacatcat ccgccagatc gcgaccagcg cgatcgcggc gaccgaagcg gatggcagct
     2701 tcgtcgaacg tattcggggc gaggacgtcg aagtcgtggc cgtgggtgga aggctgcatc
     2761 cacccctggg cgagcgcgag tcttttgagg gcagcgtggc gcagcgcgtg gtcgaaagca
     2821 atcaacccga gctgatccgg cagctgagtg aacacccgca gcggctgccc ggtgtgctgg
     2881 gcggcatgga agcagaagcg cgagcgctgg ccgtgcccct cgtggatggt ggcgaggcga
     2941 ttggcgccct ggtgttgctg cgcgacgcgc ggcgcgagcc cttcacgaag gatgaagccg
     3001 agcgtgccca cagtttcggc aacctggccg cgctcgcgtt ccgcaaggtg catctgctcg
     3061 aggacacgga acgcaggcgc gaagagctgg agcaggtcat caagagccgc tcgcgcttga
     3121 tgcgcggctt cacccacgat ctgaaaaatc cgattggtgc cgccgacggc cacgccgccc
     3181 tgctggagga tggcctgctc ggtcagctca gcgacgagca ggtgcacagc gtgcgccgca
     3241 ttcgtgcctc actggataat gcgctgaagc tgattgatgc gctcaatgaa tttgcccgcg
     3301 ccgaaaccgg ccgcatcgaa atcaggcagg cacccgtgca gctgatcgag atcgcccgcg
     3361 agctgggcga gcagtaccgc ggcatcgccg cgcagaaggg attggccgtc gacctcgagc
     3421 tgcacaaggt gcccatcatc acatcggacg acgaccgcat ccgccagatc ctcggtaatc
     3481 tgctgtccaa tgccgtgaaa ttcaccaaca cggaagggcg catcaccatg cgcacagacg
     3541 tgcgtccgaa cgagcacggt gagtgc
//
LOCUS       DASURG010000233         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_142668, whole genome shotgun sequence.
ACCESSION   DASURG010000233 DASURG010000000
VERSION     DASURG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142668"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1006)
                     /locus_tag="VFY80_03990"
     CDS             complement(47..1006)
                     /locus_tag="VFY80_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6007096.1"
                     /translation="MNLQQLRYLHGIAEHGFNISRAATALHTSQPGISRQIQLLEQEI
                     GIAILARKGNRIVGVTEPGRAVLEVARRMVQDAENLKRIGEDFTSKDSGRLVIATTHM
                     HARYVLRDVIRDFIGRHPHVQLVLRQVSPAQTAQLVATGEADIGVSGQPPGAVSELVM
                     LPCYELHRSVIAPRDHPLLAEKRLTLKSIARYPIITLDQSFVGGSAVLRAFDAAGIRP
                     NVVLSAIDADVVKTYVELGLGIAILPSIAYEPRRDTRLCAIDAAKLFEPTIACIELRH
                     ENYLRSYMLDFIQCIAPQWSRETIADARRARDVVPRAATLPLS"
     gene            1180..>2689
                     /locus_tag="VFY80_03995"
     CDS             1180..>2689
                     /locus_tag="VFY80_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6007097.1"
                     /translation="MPKMTGARLFAEMMRGYDVSHIFFVPAFMLKAFAEMEDMPIARI
                     MVHGEKAAAYMADGYARASGKPGICMAQMIGASNLAAGIRDAHMAGAPLIAITGGPTP
                     QSRYKHAYQEVDDVTQFDAVTKFNAQVDHVTRLPDLVRQAFRVATSGAPGPVHLRIQS
                     HLGQMTEQEVDLDPLVEEMYRRTPPFRPEPEGERIRAAAALLQSAQRPVIVAGGGVVR
                     SGAQAEVVALAEKLAIPVATSLNAKATLVDAHPLNVGVPGAYSRDCANRVLHEADLVF
                     FIGSHAGGQVTNNWMFPPPGIRVIQLDIDPEELGRNYPNAVSILGDAKASLKRLVDAV
                     PHSASGARKEWVDRARSIVAQWRGENAEMRDADVSPIRPERICKDISEVLPAHGIVVS
                     DTGHAGIWTARFIELRHATQSYYRTAGSLGWGFPAALGVKCAMPDRPVICFTGDGGFY
                     YHIAELETARRHNINAVIVVNNNSALNQEIKLNDIAYGGKQRGRAEEMWRFPD"
BASE COUNT          493 a          833 c          893 g          470 t
ORIGIN      
        1 cataaaccgt gttatcactg ggacaccact ctagaggctc tggatctcat gagagcggaa
       61 gcgttgccgc gcgggggacg acatcgcggg cacgccgggc atcggcgatc gtctcgcggc
      121 tccactgggg tgcgatgcac tggatgaagt cgagcatgta gctccgcaga taattctcgt
      181 ggcgaagctc gatgcaggcg atcgtcggct cgaaaagctt tgcagcgtcg attgcgcaga
      241 gccgcgtgtc gcgccgcggc tcgtaggcga tcgagggcag tatcgcgatg ccgagtccga
      301 gttccacgta ggtcttgacc acgtccgcat cgatcgcgct cagcaccacg ttcggtcgaa
      361 tgccggccgc atcgaacgcg cgcaataccg cggagccgcc gacgaagctt tgatccagtg
      421 tgatgatcgg atagcgggcg atcgatttga gcgtcagtcg tttttccgcg agcagcggat
      481 ggtccctggg cgcgatcacg ctgcggtgca gttcgtagca cggcaacatg acgagctcgc
      541 tcaccgcgcc ggggggctgg ccgctcacac cgatatccgc ttccccggtt gcgacgagct
      601 gggcggtctg cgccgggctc acctgtcgca gcacgagctg cacatgcgga tgcctgccga
      661 tgaaatcgcg aatcacgtcg cgcagaacgt agcgcgcatg catgtgtgtg gtggcgatca
      721 ccagccggcc ggagtccttg gaagtgaagt cctcgccgat gcgcttcagg ttctccgcgt
      781 cctgcaccat gcgccgtgca acttcgagga ccgcgcggcc gggctccgtc acgccgacga
      841 tgcggttgcc ctttcgcgcc aggatggcga tgccgatttc ctgttcgagc agttgaatct
      901 gccggctgat gccgggctgt gaggtgtgca gcgctgtcgc ggcacgcgag atgttgaacc
      961 cgtgttcggc gatgccgtgg agatagcgga gctgctgcag gttcatggaa gactataacc
     1021 cctgattatc gaacgataga aagagattct ttcatagcat acgatcgcgg gagtagtatt
     1081 tccagcccgg cgggtgcgtt gccgacctgc cccaaggcgc atgacgatcc cgacgacagc
     1141 gggcacctcc tcgcggaagg agcgatgccg aggacacata tgccgaaaat gaccggagcg
     1201 cggctctttg ccgaaatgat gcgcggctac gacgtgtcgc atattttctt cgtgcccgcg
     1261 tttatgctga aagcctttgc ggaaatggaa gacatgccga tcgcccgcat catggtgcac
     1321 ggcgagaagg cggccgccta catggccgat ggttacgcgc gcgcgagcgg caagcccgga
     1381 atatgcatgg cacagatgat cggggcgtcc aatctcgcgg ccggcatccg cgatgcgcat
     1441 atggcgggtg cgccgctcat tgccatcacc ggcgggccga cgccccagtc gcgctacaag
     1501 cacgcctatc aggaggtgga tgacgtgacg cagttcgatg ccgtcacgaa attcaatgcg
     1561 caagtggatc acgtcacgcg cctgccggat ctcgtgcggc aggcgtttcg tgtggcgacg
     1621 tccggcgcgc ccgggccggt ccatctgcgc attcagagcc atctcgggca gatgaccgag
     1681 caggaggtcg atctcgatcc gctggtcgaa gaaatgtatc gccgcacgcc gcctttccgc
     1741 ccggaacccg aaggcgagcg catacgcgcg gcggcggcgt tgctgcaatc ggcgcagcgg
     1801 cccgtcattg ttgccggcgg cggcgtagtg cgctccggcg cgcaagccga agtcgtggcg
     1861 ctggcggaga agctcgccat accggtcgcg acgtccctca acgccaaggc gacactggtc
     1921 gatgcgcatc cgctgaacgt cggcgtgccc ggtgcgtact cgcgcgattg tgcaaaccgc
     1981 gtgctgcacg aagcggacct cgtgttcttc atcggcagcc acgccggcgg gcaggtcacg
     2041 aacaactgga tgttcccgcc accgggtatc cgggtgatcc agctcgacat cgatccggag
     2101 gagctcggcc gcaattatcc gaacgcggtg tcgatcctcg gcgatgcgaa ggcgagcctg
     2161 aagcggctcg tcgacgccgt gccgcattcc gcctcgggcg cgcgaaaaga gtgggtcgat
     2221 cgcgcgcgca gcatcgtggc gcagtggcgg ggcgagaacg cggaaatgcg cgatgccgat
     2281 gtctcgccca tccgccccga gcgcatctgc aaggacatct ccgaagtgtt gcccgcacac
     2341 ggaatcgtgg tatcggacac cggacacgcc ggcatctgga ccgcgcgctt catcgaattg
     2401 aggcacgcca cgcagagtta ctatcgcacc gcagggtcgc tcggctgggg cttccccgcg
     2461 gcgctgggcg tgaagtgcgc catgccggac cgcccggtga tctgctttac cggagacggc
     2521 gggttctatt accacatcgc cgagctcgag accgcgcgcc ggcacaacat caacgcggtg
     2581 atcgtggtga acaacaacag cgcgctcaac caggaaatca agctgaacga catcgcctac
     2641 ggcggaaagc agcgcgggcg tgccgaggaa atgtggcgtt ttccggatg
//
LOCUS       DASURG010000234         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1061713, whole genome shotgun sequence.
ACCESSION   DASURG010000234 DASURG010000000
VERSION     DASURG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061713"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFY80_04000"
     CDS             complement(<1..1067)
                     /locus_tag="VFY80_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX6007098.1"
                     /translation="MIAPLITELPYRPDSALLFEAIADLPWAVFLDSGRHHAGQSRYD
                     ILSAQPYARLVTRGALTEIQADGIELSREDPFSLVRRFLDCGAAEGAGMPFSGGAIGY
                     FGYDLARRIERIPALARDEERMPDMAIGVYDWALVVDHCDRRTWLVGQGRDPETDVKW
                     GSLVARFTNPRPESRRVPFRITSAIASNMTREQYMHAFRRIQRYIEDGDCYQVNFAQR
                     FAAHASGDPWLAYQALRIINPAPFSAYLSTPYAQILSASPERFLRVEGGRVETRPIKG
                     TRPRTGHPRLDAELAEALRGSEKDRAENVMIVDLLRNDLSKNCALGSVKVPKLFDVES
                     FATVHHLVSTVTGELAPGRDAL"
     gene            1088..2026
                     /gene="gluQRS"
                     /locus_tag="VFY80_04005"
     CDS             1088..2026
                     /gene="gluQRS"
                     /locus_tag="VFY80_04005"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX6007099.1"
                     /translation="MNDSDIPATRERYRGRFAPSPTGPLHIGSLIAALGSYVEAKRRG
                     GDWLVRIEDVDHPRVVPGAADEILRTLDGCGMHWDGHVVRQSTHADAYHAALHELRQH
                     GLVYPCACSRREIADSGVAGIEGPVYAGICRRGLPPGKKARALRIDAAGARVSFDDAL
                     QGRIAQNLEADIGDFVLYRADRIFAYQLAVVVDDAEQGVTDIVRGADLLDSTPRQIYL
                     QDLLALPRPRYLHLPVAVNARGEKLSKQTGATPVEGPRLSAALVAALHFLGQNPADGL
                     ERAQPKSVLQWALAHWRVEHVPRARHCPAPALDSPA"
     gene            2160..4325
                     /locus_tag="VFY80_04010"
     CDS             2160..4325
                     /locus_tag="VFY80_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6007100.1"
                     /translation="MTLRSYLVLLALATLLPVAVFAVVVGVLLVDSQRDTFRHGAQER
                     TLAVLTALELELKRSISTLEALAVSGTLETSDLQDFRTIATSVLFTQRDWRTINLSLA
                     SGQQILNLNRPPGATLPLIPVEDGSVARLIESQKPVIGDMVFGPTTKQWDFAVRVPIV
                     RDGAVQYILSAVIDPGSMYDLLKTQNLPDDWIGIVLDRNGRIVARTVDPVKSVGQLAS
                     PSLREALTASLSGWYRGAEGNAFYTSYRRSEATGWTFVMNIPVYAVEQAGRQANTLLI
                     AGLLTALALALGLAVMVGRRIAKPISSLAAATQAMGLGKPLNLPAELAVSEVRTLAAA
                     LRDAERAIGERQSLIEREKEALRAADRSKDEFIAMLSHELRTPLAALTTAAHVLRMAG
                     RDDERATQARGVIERQTRHMARLVEDLLDVSRVSLGKAKLQRESFDLGELVENLIAAW
                     RAAGRFERHAFTHAIAAVWVDADRTRIEQIVSNLLDNALKFTPSGGKVSLTLRQAGAE
                     ALLQVTDEGQGLTSEAISRVFDLFVQGEQGLDRRKGGMGIGLALVRRLSEMHGGSVSV
                     TSKGPGAGATFTVRLPAIVRPRRDLLDRAGIEEPLRTRRVLIVEDNDDTRQMLRAALT
                     LKGHEVDEAADGATGLAVALASPPDIALIDIGLPDIDGCELARRLRASAQNGRIPLIA
                     LSGYGQPEDLKRAYDAGFDSHLTKPVAIEELHSAITALR"
     gene            4322..4702
                     /locus_tag="VFY80_04015"
     CDS             4322..4702
                     /locus_tag="VFY80_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007101.1"
                     /translation="MTRPGRRLLIVDDDADMRLSLKLALEMAGYDAVVAADGREALAL
                     QKQHPADILITDIFMPESDGFEVIDAVRRGFPQTRIVVVSGGARLAKREYLLDAALMD
                     VDAILPKPFDVETLLKTLDTIQKR"
     gene            complement(4699..>4936)
                     /locus_tag="VFY80_04020"
     CDS             complement(4699..>4936)
                     /locus_tag="VFY80_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007102.1"
                     /translation="LARGAGYPRAYAFSELEEFSSALPGILSEPGPVFVAMKVHPEVE
                     NRPIGQRSKWMRKTREQAIADLQHEVAKRERAAV"
BASE COUNT          876 a         1597 c         1631 g          832 t
ORIGIN      
        1 agcgcatcgc gaccgggcgc gagctcaccg gtcaccgtgc tgacgagatg atgcacggtg
       61 gcgaagcttt cgacatcgaa gagcttcggc acctttaccg acccgagcgc gcaattcttg
      121 gaaagatcgt tgcggaggag atccacgatc atcacattct cggcgcgatc cttttcgctc
      181 ccgcgcaagg cttcagcgag ctccgcatcg agccggggat ggcccgtccg cggccgcgtg
      241 cctttgatcg gacgcgtttc gacgcgcccg ccttcgactc tcaggaagcg ctcgggagaa
      301 gcagacagga tctgcgcata aggcgtgctg agatacgccg aaaaaggcgc cgggttgatg
      361 atgcgcagcg cctgataggc gagccagggg tcgccgctcg cgtgcgcggc gaaacgctgc
      421 gcgaagttga cctggtagca gtcgccgtct tcgatatagc gctgaattct gcggaacgcg
      481 tgcatatact gctcgcgcgt catgttggac gcgatcgcag aagtgatgcg aaacggcacc
      541 cgacgcgatt ccggccgagg attcgtaaag cgggcgacga gactgcccca tttgacatca
      601 gtctcgggat cgcggccctg ccctacgagc caggtgcggc ggtcgcagtg atccacgacg
      661 agcgcccagt cgtagacgcc gatcgccatg tcgggcattc gctcctcgtc gcgtgcaagc
      721 gccgggatgc gctcaatgcg gcgcgcgaga tcatagccga agtagccgat cgcgccgccg
      781 ctgaagggca tgcctgcgcc ctctgccgcg ccgcagtcga gaaaacggcg cacgaggctg
      841 aacggatcct cgcgcgaaag ctcgatgccg tccgcctgta tctcggtcag cgcgccccgc
      901 gtgacgaggc gggcgtaggg ctgtgccgac aggatgtcat agcgcgactg gccggcatga
      961 tgccggccgc tgtcgagaaa aacggcccac ggcagatcgg cgatcgcctc gaacagcaac
     1021 gcgctgtcgg gccgatacgg cagttcggta ataagcggcg caatcacgct cctataatag
     1081 cggagcgatg aacgattccg atatcccggc gacacgcgaa cgatatcgcg gtcgcttcgc
     1141 accctccccc accggcccac tccatatcgg ctcgctcatc gccgcgctcg gcagttacgt
     1201 agaagcgaag cgtcgcggcg gcgattggct cgtgcggatc gaggatgtcg atcacccgcg
     1261 cgtcgtgccg ggtgcggccg acgagattct gcgcacgctc gacggatgcg gtatgcactg
     1321 ggacgggcac gtcgtccgcc agagcacgca tgccgacgcc tatcatgcgg cgttgcatga
     1381 actgaggcaa cacggccttg tttatccgtg cgcctgcagc cggcgcgaga tcgcggattc
     1441 aggcgtcgcg ggaatcgagg ggccggtgta cgcgggcatc tgccggcgcg gcctgccgcc
     1501 cggaaaaaaa gcacgtgcac tgcggatcga tgctgccggc gcacgcgtaa gttttgacga
     1561 cgcgttgcag gggcgcattg cgcagaatct cgaggcagac atcggggatt tcgtgctgta
     1621 tcgcgccgac cgcatttttg cataccagct cgccgtggtc gtggatgatg cggaacaggg
     1681 cgtgaccgac atcgtgcgcg gcgccgatct cctggattcc acaccccgtc aaatctatct
     1741 ccaggatctg ctcgccctgc cgcggccgcg ttatctgcac cttcctgttg cggtaaacgc
     1801 gcgcggcgag aaactgagca agcagacggg tgcaacgccg gtcgagggcc cccgtctctc
     1861 ggctgcgctt gtggcggctc ttcactttct cggacagaac cctgcggacg ggctcgagcg
     1921 ggcccagcct aagagtgtcc tgcaatgggc tctcgcccac tggcgagtcg agcacgtgcc
     1981 acgcgcccgg cactgccctg ctccggccct cgattcgccc gcatagcgaa cgaatattca
     2041 gaaagacgcg ttgccttcat gaatcaaacg gaacagaatg aagtgcatcc gtacgtcttt
     2101 ccggtccggt gctcaaggag gagatgccgc tcgcgcgtgc gcggcatgta gacgaaagaa
     2161 tgaccctgcg ttcctatctg gtgctcctgg cgcttgcgac gctgctgccg gttgccgttt
     2221 tcgcggtggt ggtcggggtg ctgctcgtgg acagccagcg cgataccttt cggcacgggg
     2281 cacaggagcg gaccctcgcg gtgctgaccg cgctcgaact ggaactgaag aggtccatct
     2341 ccacgcttga agcgcttgcc gtctccggca ccctcgagac atccgatctc caggacttcc
     2401 gcaccatcgc gacgagcgta ctctttacgc agcgggactg gcgcaccatc aatctgtcgc
     2461 ttgcgtctgg acagcagatc ctcaacctga accgcccgcc cggcgcaacg ttgcctctga
     2521 tcccggtgga agacggcagc gtcgctcgcc tcattgagag ccagaaaccg gtgatcggcg
     2581 acatggtgtt cgggccgacc acgaagcagt gggatttcgc ggtccgcgtg ccgatcgtgc
     2641 gcgatggcgc cgtccagtac attctgtccg cagtcatcga tccgggctcc atgtacgacc
     2701 tgctcaagac gcaaaacctg ccagacgact ggatcggcat cgtgctcgat cgcaacggtc
     2761 gcatcgtggc gaggaccgtg gatcccgtaa aaagcgtcgg gcaactcgca tcgccgagcc
     2821 tgcgcgaggc gctgacggcg tcgctcagcg gctggtatcg cggggccgaa ggcaatgcgt
     2881 tttatacgtc gtatcgccgt tccgaagcga ccggctggac cttcgtgatg aacattccgg
     2941 tctacgccgt cgagcaggca ggcagacagg cgaacaccct gctcatcgcg gggctcctca
     3001 ccgctctcgc gcttgctctt ggcctcgcgg tgatggtcgg tcggcgcatc gccaagccga
     3061 tttcctcgct cgccgcggcc acccaggcga tgggactggg caaaccactc aatttgccgg
     3121 ccgagcttgc ggtcagtgaa gtccgcacgc tcgctgccgc actgcgcgac gcggaacgcg
     3181 cgatcggcga gcgccagagt ctcatcgagc gtgaaaagga agcgttgcgg gcggccgacc
     3241 gcagcaagga tgaattcatc gcgatgctga gccacgagtt gcgcacgccg ctcgccgccc
     3301 tcaccaccgc cgcacacgtg ctgcgcatgg cgggcaggga cgacgagcgg gccacgcagg
     3361 cgcgcggggt catcgagcga cagacgcggc acatggcacg gctcgtggag gatctgctcg
     3421 atgtctcgcg cgtatcgctc gggaaggcca agctccagcg cgagagcttc gatctcggcg
     3481 agctcgtgga gaacctgatt gcggcatggc gcgcggcggg gcgcttcgag cggcacgcgt
     3541 tcacccacgc gatcgcggcg gtatgggtgg acgccgaccg cacgcgcatc gagcaaatcg
     3601 tctcgaacct gctcgacaac gcgctcaagt tcacgccgtc cggcggcaag gtgagcctca
     3661 cgttgcgcca ggcaggcgcc gaggcgctgc tgcaggtcac ggatgaaggc cagggcctca
     3721 catccgaagc gatcagccgc gtgttcgatc tcttcgtgca gggcgagcag ggactggacc
     3781 gccgcaaagg cgggatgggt atcgggctcg cgctggtgag acgcctgtcg gaaatgcatg
     3841 gcggaagcgt gtcggtcacg agcaagggcc cgggagcggg ggcgacgttc accgtgcggt
     3901 tgccggcaat cgtgcgaccg cggcgggatc tcctcgatcg cgcggggatc gaggagccgc
     3961 ttcgcacgcg gcgcgtcctg atcgtcgagg acaacgacga tacgcgtcag atgctgcggg
     4021 ccgcgcttac gctgaagggg cacgaagtgg acgaagcggc cgatggcgca acggggctcg
     4081 cggtcgcgct cgcctcgccg cccgatatcg cgctcatcga catcggcctg ccagacatcg
     4141 atggctgcga gcttgcgcgg cgcctccggg cgagcgcgca gaacggccgc atcccgctca
     4201 tcgctctgag cggctatggc cagcctgaag atctcaagcg cgcgtacgac gcgggcttcg
     4261 actcgcatct caccaagccg gtggcgatcg aagagttgca cagcgccatc accgccttgc
     4321 gatgacccgg cccggtagac gccttctgat cgtggacgat gacgccgaca tgcggctctc
     4381 gctcaagctc gcgctggaga tggcgggata cgacgcggtg gtcgcggcgg acgggcggga
     4441 agcgctcgcg ctgcagaagc agcatcccgc agacatcctc atcaccgaca tcttcatgcc
     4501 ggagagcgac ggattcgaag tgattgacgc cgtgcgccgc ggctttcccc agacgcgcat
     4561 cgtcgtggtg tcgggcggcg cacgactcgc caagcgcgaa tacctgctcg acgcagcgtt
     4621 gatggacgtg gatgcgatcc tgccaaagcc gttcgacgtc gagacgctgc tcaagacgct
     4681 cgacaccatt cagaaacgct agacggcggc acgctcccgt ttcgccacct cgtgctggag
     4741 atccgcgatc gcctgctcgc gcgtcttacg catccacttg gagcgctgac cgatcggcct
     4801 gttctccact tcggggtgca ctttcatcgc gacgaatacg ggtcccggct cgctcaggat
     4861 gccggggagt gcgctcgaga attcctcgag ctcgctgaat gcgtaggccc tcggatagcc
     4921 ggcgccgcgc gcaagg
//
LOCUS       DASURG010000235         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_551892, whole genome shotgun sequence.
ACCESSION   DASURG010000235 DASURG010000000
VERSION     DASURG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551892"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFY80_04025"
     CDS             <1..1005
                     /locus_tag="VFY80_04025"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX6007103.1"
                     /translation="VKAQVRSGGRGKQGGVVKVGDAAALTAAIERLFAINFGGERPEA
                     LLIEPWLAIDRELYLSVTVDGRADGYVVMYAPRGGINIEDGPPPARYEVGAAHCFRVH
                     ELRAVLAAHEADYALREKVVALAQRLIRTATLHDCTTIEINPLVRLADGDIIAADAKV
                     VRDEWAAFRNREIAEHIEENKRRADRLLRGCLDMQHMYVRLDGDIALISGGAGMTMAA
                     MDMIAEHGGRPACFLDCSPGPTSTRGYRPAFAMLDADSQVKVILVSVFGGGTQMQRVA
                     NAMKELMAERRSRKPVVFRLDGTNVDQVPGIFSQFGARNHACLEDAVSEAVTLAKGAQ
                     "
     gene            1002..1904
                     /locus_tag="VFY80_04030"
     CDS             1002..1904
                     /locus_tag="VFY80_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6007104.1"
                     /translation="MSILLDRSNRVLIQGITGQMGTFFVEDARKYGTQIVAGVSPGRD
                     GTRVGDVPVFGNVRDARAATGADTAIVFVPPAAVLGATLEAIESGCRLVVATADELPV
                     QDAIEIRAAAKAHGAHFVGPNTPGLISPGKAKLGFMPSFCYREGSVGVISRSGSLSYE
                     CCKRLTLAGIGQSTVVGIGGDPVKGTTAAEALELFHRDPETSAVLYLGEIGGTEETFV
                     AEYAARAGAKPVAALIVGYTAPPGKKMGHAAALIGSKAESHAAKVAMLRAAGVHVADG
                     LSNVVPTLQAALASGMQPTQGIAA"
BASE COUNT          358 a          694 c          707 g          321 t
ORIGIN      
        1 gtgaaagcgc aggtccgcag tggcggccgc ggcaagcagg gcggcgtcgt gaaggtggga
       61 gatgccgcgg cgctcaccgc ggcgatcgag cgtctcttcg cgataaattt cggcggagag
      121 cggcccgagg cgctgctgat cgagccgtgg cttgcgatcg atcgcgagct ctacctgtcg
      181 gtcaccgtgg acggacgcgc ggacggctac gtcgtcatgt acgcgccgcg gggcggcatc
      241 aacatcgagg atggccctcc acccgcccgc tatgaggtcg gcgcagcgca ttgcttccgt
      301 gtccacgagc tgcgcgccgt gctcgcggcg cacgaagcgg attacgcatt gcgcgagaag
      361 gtcgtcgcgc ttgcgcagcg tctcatccgc accgccaccc tccacgactg cacgacgatc
      421 gagatcaacc cgctcgtgcg gctcgcggac ggcgacatca tcgccgccga cgccaaggtg
      481 gtgcgcgatg aatgggcggc gttccgcaat cgcgagattg ccgagcacat cgaagagaac
      541 aagcggcgcg ccgaccggct gctccgcggt tgcctcgaca tgcagcacat gtacgttcgg
      601 ctcgacggcg acatcgcgct catctccgga ggggcgggca tgacgatggc ggcgatggac
      661 atgatcgccg agcacggcgg tcggcccgcg tgctttctcg actgcagccc cggccccacc
      721 tcgacgcgcg gctaccggcc cgccttcgcc atgctcgatg cggattccca ggtcaaagtg
      781 atactcgtga gcgtcttcgg cggaggcacg cagatgcagc gcgtcgcgaa cgcgatgaag
      841 gagctcatgg cggagcgcag gagccgcaag cccgtggtgt tccggctgga cggcaccaac
      901 gtcgaccagg ttcccggcat cttcagccag ttcggcgcgc gcaaccacgc ctgcctcgag
      961 gacgccgtct ccgaagcggt cactctcgca aaaggagcgc aatgagcata ctgctcgatc
     1021 gcagcaaccg ggtgctcatc cagggcatca ccggccagat ggggaccttc ttcgtggagg
     1081 acgcgcgcaa gtacggcacg cagatcgtcg cgggcgtgtc cccgggacgc gacggcacgc
     1141 gcgtgggaga cgttccggtc ttcggaaacg ttcgggatgc gcgcgcggca acgggcgcgg
     1201 atacggcgat cgtgttcgtc ccgcccgccg cggtgctggg cgcaaccctc gaagccatcg
     1261 aatccggctg ccgtctggtg gtggccaccg cggatgaatt gccggtccag gacgccatag
     1321 agatccgcgc cgcagccaag gcgcatggcg cacacttcgt cggtcccaat acgccgggac
     1381 tcatttcgcc gggcaaggcc aagctcggct tcatgccttc gttctgctac cgcgaaggca
     1441 gcgtcggcgt catctcccgc agcggctcgc tctcgtacga gtgctgcaag cggctgacgc
     1501 tcgccggcat cggccagtcc acggtggtgg gcatcggcgg cgacccggtg aagggcacca
     1561 cggccgcgga ggcgctcgag ctcttccatc gcgatcccga aacgagcgcg gtgctgtacc
     1621 tgggcgagat cggcggcacc gaggaaacgt tcgtggcgga gtatgccgcg cgcgcaggag
     1681 cgaaaccggt ggcggcgctg atcgtcggct atacggcgcc gcccggcaag aaaatgggcc
     1741 acgctgcggc cctcatcgga tcgaaagcgg aaagccatgc agccaaagtc gcgatgctgc
     1801 gcgcagccgg cgttcacgtc gccgacgggc tttccaatgt ggttccaacg ctgcaggccg
     1861 cactggcttc gggcatgcag ccgacgcaag gcatcgccgc atagccaaaa atatccatac
     1921 cgaggaggac gtcacatgaa aatccaactt ccgcttgtgc ttctgggatt gagcgccgcg
     1981 gtttccgcgc agggcgtcgc cgcagccgag atgccctacc ccacgaagcc gatacgcatc
     2041 gtcgtcggct ttccgccggc gggcgccgcg gacatctttg
//
LOCUS       DASURG010000236         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_552097, whole genome shotgun sequence.
ACCESSION   DASURG010000236 DASURG010000000
VERSION     DASURG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552097"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFY80_04035"
     CDS             <1..858
                     /locus_tag="VFY80_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6007105.1"
                     /translation="IAPIYVVGQYKPQSLVRHIDTAWDRWSTGPEAKIRIVLPGANGS
                     QSQFKGTADVVYQNLRLIAHHRPDIVAVFAADHVYRMDVRQMVDFHVARAADVTVAAV
                     PVPLAEASSFGVLVTDERGSIREFQEKPERPAPSPHDPERAHASMGNYLFDPDVLIGV
                     LEAAQHRGAVDFGCDVMPSLPQCCRVLAYDFTSNEVPGVKPYEERGYWRDVGTIASLA
                     AARSDVIGPRPRFDLWNRRWPIHGEGHTERFHSLSEWTEHPVRLNPERAAARGSGIAR
                     HTPGPVLPS"
     gene            complement(785..2263)
                     /gene="glgA"
                     /locus_tag="VFY80_04040"
     CDS             complement(785..2263)
                     /gene="glgA"
                     /locus_tag="VFY80_04040"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX6007106.1"
                     /translation="MISGDAPRVLFVTPECAPLAQVGGLGDVSAALPPALRAVGTDAR
                     VLMPGYRRVLASLHALEPIARFSALEPPREAVLLEGRLPTGVPVYVLDHAPLYDRDGD
                     PYQDVLRRDWPDNALRFALLSHVAAQLGGADSPLAWQPHVVHLNDWPAGLAAAYLHYM
                     PQRRAASVFTAHNLAFQGNFDAGLRSGLALPPESYAPEGLEFYGQVSFLKAGLFFADA
                     ITTVSPTYSREFQTEPFGAGMHGVLQKRRGVLHGILNGIDTAVWDPATDPHLPVRYSR
                     DTLDAKRHVKRALQQRLSLPVAPQDVPLIGMVGRLTHQKGIDLVLEAVPHLAEMAQLA
                     LVGAGAPEHEDALCGVAARFPQRIGVVIGFDEALAHLIEGGADMFLMPSRFEPCGLNQ
                     MYSQRYGTPPIAHATGGLADTIVDCTPATLAAGTATGFLFTAPRVDALQEAIARACAV
                     YRASDAWHRLQRNGMARDFSWAGPARAYAALYRSLVRQRARG"
     gene            complement(2312..>4167)
                     /locus_tag="VFY80_04045"
     CDS             complement(2312..>4167)
                     /locus_tag="VFY80_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6007107.1"
                     /translation="YSVWEAFLYGPPASALANQTLLVLVTFNSTVAITGLVLCAVLNE
                     RGRNVAALRASRDELESRVRERTIELQRANRALQLDIASRIRTEKLLLESEERFRQMV
                     ASVVDYAIFMLDTDGRVASWNAGAARIKRYTAEEIIGRHFSCFYPAADVDSGKPWRHL
                     EVAAAEGRVEDEGWRIRKDGSTYWASVVLTAVRDASGKLIGFSEVTRDLTDRLRAEEE
                     LRRGEARFGALVNSVVDYAIIMLDPDGRISSWNTGAEHTLGYSAEQIIGRHFSVFYYR
                     EDIERGKPQRELEDAISLGRHEDEGWRIRKDGSRFWANVVVAAIRDGSGQVLGFSKVM
                     RDLSERKRLEVELTDARAVAEQSNRMKTEFLAKMSHELRTPLNSLLILARLLAENAPG
                     NLNAKQVQYAQTIHAAGTDLLELINDLLDLAKIESGTVVLTLTPERFSDLVDYTRHTF
                     EQTAQSKGLQFDITVDERLPETLSTDARRLHQILRNLLANAFKFTHRGRVALDIALAN
                     SGWTPGHPQLEAADAVVAFSVSDTGIGIPPDKKETVFEAFQQLDQGTSRQYGGAGLGL
                     PISRELTRLLGGDLLVHSEAGRGSTFVLYLPLAASAPLAETNSKMLEQAAR"
BASE COUNT          714 a         1374 c         1357 g          722 t
ORIGIN      
        1 atcgcgccga tctacgtggt gggtcagtac aagccccagt cgcttgtacg ccatatcgat
       61 acggcgtggg atcgctggtc cacgggcccg gaagcgaaga ttcgcatcgt gctcccgggc
      121 gcgaacggga gtcagtcgca attcaagggc acggcggatg tggtctatca gaacctgcgc
      181 ctcatcgcgc accaccggcc cgatatcgtc gcggttttcg ccgcggatca cgtgtaccgc
      241 atggacgtgc ggcagatggt cgattttcat gttgcgcgcg ccgccgacgt gacggttgcc
      301 gcggtgccgg tgccgctagc agaagcgtcc tcgtttgggg tcctggttac ggacgagcgc
      361 ggctccatcc gcgaatttca ggaaaagccc gaacggcccg cgccgtcgcc gcacgaccct
      421 gagcgcgcgc acgcttcgat gggaaattac ctgttcgatc cggatgtgct gattggcgtg
      481 ctggaggcgg cacagcatcg cggcgccgtc gatttcgggt gcgacgtcat gccgagcttg
      541 ccgcagtgct gccgcgtgct cgcctacgat ttcacgagca atgaagtgcc cggcgtgaag
      601 ccttacgagg aacgagggta ctggcgcgat gtcggcacca tcgcgagcct tgccgccgcg
      661 cgcagtgacg tgatcgggcc gcgccctcgt ttcgacctgt ggaaccgccg ctggccgatc
      721 cacggggaag gccacaccga acgcttccac agcctgagcg aatggaccga gcacccggtg
      781 cggctcaacc ccgagcgcgc tgccgcacga ggctccggta tagcgcggca tacgcccggg
      841 ccggtcctgc ccagctgaaa tcccgtgcca tcccgttgcg ctgcaggcgg tgccaggcgt
      901 ccgacgcgcg gtatacggcg cacgcgcgtg ctatggcttc ctgcaaagca tcgacccgtg
      961 gggccgtgaa cagaaatccg gttgcagtcc ccgccgcgag cgtcgccggg gtgcagtcca
     1021 cgatggtgtc cgccagcccc cctgtggcat gcgcaatcgg cggcgtgccg tagcgctggc
     1081 tgtacatctg gttcagcccg cacggctcga agcgcgaggg catcaggaac atgtcggcgc
     1141 cgccttcgat cagatgagcg agcgcctcgt cgaagccgat cacgacgccg atccgctgcg
     1201 gaaagcgggc cgcgacgcca cacaaggcgt cctcgtgttc gggcgcgccc gcgccgacca
     1261 gcgcgagctg cgccatctcg gcaaggtgtg gcaccgcttc gagcacgaga tcgatcccct
     1321 tctggtgcgt gagccggccg accatcccga tgagcggtac gtcctgcggg gcaacgggca
     1381 gagacagtcg ctgctgcagg gcgcgcttca catgccgttt cgcgtccagc gtgtcgcggc
     1441 tgtagcgaac cggcaagtgt ggatcggtcg ccggatccca tacggcggtg tcgatgccgt
     1501 tcaggatgcc atgcagcaca ccgcggcgct tctgcagcac gccgtgcata ccggccccga
     1561 agggctcggt ctggaattcc cttgaataag tcgggcttac ggtcgtgatc gcgtccgcga
     1621 aaaagaggcc ggccttcaga aacgacacct gcccgtagaa ttcgaggccc tccggcgcgt
     1681 aactctcggg cggtaatgcg agcccgcttc gcaggcccgc gtcgaaattg ccctggaacg
     1741 cgaggttgtg cgcggtgaac acgctggccg cacggcgctg tggcatgtaa tggaggtagg
     1801 cggcggcgag ccccgccggc cagtcgttca gatgcacgac gtgcggttgc cacgcgagcg
     1861 ggctgtcggc gccgcccaac tgcgcggcga catgcgacag gagggcgaat cgcagcgcat
     1921 tgtcgggcca gtcccgacga agcacgtcct gatacgggtc gccgtcgcga tcgtacagcg
     1981 gcgcgtggtc gagcacataa acgggaacgc cggtcggaag acgcccttcg agcaatacag
     2041 cctcgcgcgg aggctcaagt gcgctgaacc gcgcgatcgg ctcgagcgca tgcaaggaag
     2101 cgaggacgcg ccgatagccc ggcatcagca cgcgggcatc ggtcccgacc gcgcgtagtg
     2161 ctggagggag cgctgcgctc acgtcgccca gtccaccgac ctgggcgagc ggcgcgcatt
     2221 ccggcgttac gaaaaggact cgcggtgcgt caccgctgat cacttaatcg ctttcatgac
     2281 gatgcggcgg tcggccgtga gcgcggcggg atcagcgagc ggcctgctcg agcattttcg
     2341 agttcgtttc cgccagcggc gcgctcgccg cgagcggcag atagagcacg aacgtgcttc
     2401 cgcgaccggc ctcgctgtgt acgagcaggt ccccgccgag cagccgcgtg agttcgcggg
     2461 agatcggcag cccgagcccg gcaccgccat actgccgact cgttccctga tcgagctgct
     2521 ggaacgcttc gaacaccgtt tctttcttgt cgggcggaat cccgataccg gtatcgctga
     2581 ccgaaaaggc aacgaccgcg tcggccgcct ccagctgcgg atgccccggc gtccagcccg
     2641 aattcgcgag cgcaatatcg agcgcaaccc gtccgcggtg cgtgaacttg aacgcgttgg
     2701 cgagcagatt acgcaggatt tgatggagtc gtcgcgcgtc cgtactgagc gtctccggca
     2761 agcgctcgtc gacggtaatg tcgaattgca ggcccttgga ctgcgcggtc tgctcgaacg
     2821 tgtgtcgggt gtaatccacg agatcagaaa aacgctcggg tgtcagggtg agcacgaccg
     2881 tgcccgattc gatcttcgca agatccagca ggtcgttgat gagctccagc aaatcggttc
     2941 ccgcggcatg gatagtctgc gcgtactgga cctgctttgc gttgagattc cccggcgcgt
     3001 tctcggcgag caaccgcgcg aggatgagca gactgttgag cggcgtgcgc agctcgtgcg
     3061 acatcttggc gagaaattcg gttttcatgc gattcgactg ctcggccacg gcacgtgcgt
     3121 ccgtaagctc gacttcgagc cgcttgcgct cgctcagatc gcgcatgacc ttcgagaaac
     3181 cgagcacctg gccgcttccg tcccggatcg ccgccaccac cacgttggcc cagaagcgcg
     3241 agccatcctt gcggatgcgc cacccctcgt cttcatggcg tcccagcgaa atcgcgtctt
     3301 cgagctcacg ctgcggcttt ccgcgctcga tgtcttcgcg gtaatagaac acggaaaagt
     3361 gccgtccgat gatctgctcg gcactgtatc ccagcgtgtg ctcggcgccg gtattccagc
     3421 tgctgatgcg accgtcggga tccagcatga tgattgcata gtccaccacg ctgttcacca
     3481 gcgccccgaa gcgcgcctcg ccgcgccgca gctcctcttc cgcgcgcaag cggtcggtga
     3541 gatcgcgcgt cacttccgag aatccgatga gcttgcccga ggcgtcgcgc acggccgtca
     3601 acacgacgct cgcccagtat gtcgagccgt ccttgcggat gcgccagcct tcgtcctcga
     3661 cgcggccttc ggccgcggcg acttccaggt ggcgccatgg cttcccgctg tctacatcag
     3721 cggccggata gaagcaggag aaatgccggc cgataatctc ctccgccgtg taacgcttga
     3781 tgcgcgccgc gccggcgttc cagctcgcga cgcgaccgtc cgtgtcgagc atgaatatcg
     3841 cgtagtccac cacgctcgcc accatctggc ggaaacgctc ctcgctctcg agcagcagtt
     3901 tctcagtgcg gatgcgcgag gcgatgtcga gctggagggc gcgattggcg cgctgcagct
     3961 cgatggtccg ctcgcgtacc cgcgattcca gctcatcgcg gctcgcacgc agtgctgcca
     4021 cgttacgccc acgctcgttc aggactgcac acagcacgag cccggtaatg gcgacagtgc
     4081 tgttgaaagt caccagaacg agaagcgtct ggttcgccag ggcgctcgca gggggtccat
     4141 acagaaatgc ctcccaaacc gaataga
//
LOCUS       DASURG010000237         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_552152, whole genome shotgun sequence.
ACCESSION   DASURG010000237 DASURG010000000
VERSION     DASURG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552152"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VFY80_04050"
     CDS             complement(<1..885)
                     /locus_tag="VFY80_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007108.1"
                     /translation="MEKSFPVVHCLAAAALLWTAVADARESAALAPYPVKPIRVITGS
                     SPGTSDDFFARAIGEELSALYRQRIIVDNRSGAGGLIGNQILSRANPDGYTLGLIGVT
                     RIITALMREEAPYRVLDDITGVAHIASITNVLAVTPALAVESPRQFVRHARARAGELN
                     YASLGIGSASHLAGEVFTRAVGIDAVHVPFRHLSDSFVEMVLGRVHYAVLTLPAVLGS
                     LRDGRMRALAVMTPERSTALPEVPAIAEYGLPEAQFDSWSGIVAPKGTPRRIVEQLHG
                     DIVRVLRRREMRELFARQG"
     gene            complement(885..2072)
                     /locus_tag="VFY80_04055"
     CDS             complement(885..2072)
                     /locus_tag="VFY80_04055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6007109.1"
                     /translation="MDFTLPEELRMLRETVARFAREELRPLEKDVIRREAERGLTDAP
                     LIDPEAEKALNRKAKEIGLYGIDVPEEYGGQNLGMLAKAVVIEELKTSITPYVLPPDS
                     PNLYMLREQCKGEQIQKYFIPYAKGEKKSAIAITEPGAGSDPAAMKMRAEYRNGKWVL
                     NGQKIFISNARKADFLIVMAVTDPSKGSRGGITAFLVDQGTKGLSIPTVFNTIGEHAP
                     YGVFFDDVEVPDDQVLGAIGNGFGPMQNRLGVRRMEIACRSLGFARRCMELMIPYANE
                     RVTFGKPLAERQAVQWWIADSFQEMEMVRLITWRLACKIDKGESNWRRDAAMVKLQGS
                     EMIARVADRAMQLFGGMGMTKDLPLEYIARACRAYRVYEGPSEVHRWFIARDLLRNGM
                     PVE"
     gene            2355..>2556
                     /locus_tag="VFY80_04060"
     CDS             2355..>2556
                     /locus_tag="VFY80_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX6007110.1"
                     /translation="MRRFYAATAALLVFAVTAIGCAPHWTDEGWTRVIEGQKGLEHFD
                     RVGDANWRGEHGYIVADQGKGGY"
BASE COUNT          434 a          811 c          848 g          463 t
ORIGIN      
        1 gccctgacgt gcaaaaagct cgcgcatctc gcgcctgcgc agcacgcgca cgatgtcgcc
       61 gtgcaactgc tccacgatgc ggcgcggtgt gcccttcgga gcgacgatcc ccgaccagct
      121 gtcgaactgc gcttccggca atccgtattc cgctatcgcg ggcacttcgg gcagcgcggt
      181 gctgcgctcg ggggtcatca cggcgagcgc ccgcatgcgc ccgtcgcgca gggaaccgag
      241 aacggcgggc agtgtgagca ccgcatagtg cacgcgccca agcaccatct cgacgaagga
      301 atccgacaga tgacggaagg gaacgtgcac ggcgtcgatg ccgaccgcgc gcgtgaagac
      361 ctcgccggcg aggtgcgatg ccgacccgat gccgagcgag gcgtaattga gctctccggc
      421 ccgggcgcgc gcatgccgca cgaactggcg cgggctctcg accgcaagag cgggcgtgac
      481 ggcgagcacg ttggtgatcg aagcgatgtg cgccacgccg gtgatgtcgt ccagcacgcg
      541 gtagggcgct tcttcgcgca tgagcgccgt gataatgcgc gtgacgccta tgaggcccag
      601 cgtgtagccg tcgggattcg cgcgggacag gatctggttg ccgatgagac cgcctgcgcc
      661 ggagcggttg tccacgatga tgcgctggcg gtagagcgcg ctcaactctt cgccgatggc
      721 gcgcgcgaag aagtcgtccg aagttccagg cgaggagccg gtgatgacgc gtatcggttt
      781 gacgggatag ggcgcgagcg cagccgactc gcgcgcatcg gccactgccg tccatagaag
      841 cgcggcagcc gcgagacagt ggacgaccgg aaaggatttt tccatcactc gaccggcatg
      901 ccgttgcgaa gcaggtcgcg cgcgatgaac cagcggtgca cctcgctcgg cccttcgtag
      961 acgcgatacg cgcggcaggc acgcgcgatg tattcgagcg gcagatcttt cgtcatgccc
     1021 atgccgccga aaagctgcat agcgcggtcg gccacgcgcg cgatcatctc gcttccctgc
     1081 agcttcacca tcgcggcgtc gcgccgccag ttgctctcgc ccttgtcgat cttgcacgcg
     1141 agccgccagg tgatgaggcg caccatctcc atttcctgga agctgtcggc gatccaccat
     1201 tgcacggcct ggcgctccgc gagcggtttg ccgaaggtca cccgctcgtt cgcgtacggg
     1261 atcatgagtt ccatgcagcg gcgcgcgaac ccgagcgacc ggcacgcgat ctccatgcgg
     1321 cgcacgccga gccggttctg catcggacca aagccgttcc cgatggcgcc cagcacctga
     1381 tcatcgggaa cttccacgtc gtcgaagaaa acgccgtagg gcgcgtgctc gccgatggtg
     1441 ttgaagaccg tcggtatgga caagcccttg gtgccctgat cgacgagaaa cgcggtgatg
     1501 ccgccgcgcg agcccttcga tggatcggtc accgccatca cgatgaggaa atccgccttg
     1561 cgggcgttgc tgatgaagat cttctggccg ttgaggaccc atttgccgtt gcggtactcg
     1621 gcgcgcatct tcatcgccgc cggatcggaa ccggcgccgg gctcggtgat cgcgatggcg
     1681 ctcttcttct cgcccttcgc gtacgggatg aagtacttct ggatctgttc gcccttgcac
     1741 tgctcgcgca gcatgtagag attgggactg tcggggggca gcacgtaggg cgtgatgctg
     1801 gttttcagtt cctcgatgac gaccgctttc gccagcatgc cgagattctg cccgccgtat
     1861 tcttccggca cgtcgatgcc gtagagcccg atttccttcg ccttgcggtt gagcgccttc
     1921 tccgcctcag ggtcgatgag cggcgcatcg gtcaggccgc gctcggcttc gcgccggatc
     1981 acgtccttct cgagcgggcg cagttcctct cgcgcaaagc gggcgaccgt ttcgcgcagc
     2041 atgcgcagtt cttcgggtag ggtgaagtcc atgtcgtggc cttgaactgg aagttcgggc
     2101 ggtgacgagc atcataacaa cagcgtgcgt aaagccgaaa tcccgggcgg cgcgagggcg
     2161 tgctgccgtc cgcgatctgg acggtgcgtc agcgccgacc ccgtgcatgc tcggcacaca
     2221 ccattctttg ccggctccac caggacacgc cgggagcgct gtggcgcgcg cttgactgtg
     2281 gcgcgcttcg attacatgct ttagccgctg gagatcgagt acatcaacaa ctcgcatcac
     2341 gacagggaga catgatgaga cgcttctacg ctgcgaccgc ggcgcttctg gtattcgctg
     2401 ttaccgccat tgggtgcgcc ccgcattgga ccgatgaagg ttggaccagg gtcatcgagg
     2461 gtcaaaaggg gctggaacat ttcgatcgcg tgggcgacgc caactggcgt ggcgagcacg
     2521 gctatatcgt ggcggatcag ggcaaaggtg gctacc
//
LOCUS       DASURG010000238         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1062323, whole genome shotgun sequence.
ACCESSION   DASURG010000238 DASURG010000000
VERSION     DASURG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062323"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFY80_04065"
     CDS             <1..845
                     /locus_tag="VFY80_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6007111.1"
                     /translation="ADALLEAGVGKGRIAIELDFMPAKDFLQLRKRLPEATFEECAPI
                     FFRTRMIKSDEEVSTLRQVGTLSERVIHDVFKEVRVGMTEIDVARLIVDQMIAGGSSN
                     FKYRVGSGVNSSITNCGTTLKKIEAGDLIRVEVLGEIDNYRSNVTRTAVVGKPTERQT
                     NIWSTLMRAREVCKTMLKPGTPVADVYRTYVRMCRENGIEPTLKFLGHGIGQTIHEEP
                     YLTDTRDIVFEPNVTFTMEPLYMIPGEMGFHVEDMYVITSVGFDPITGNICNNDTLIE
                     VGRA"
     gene            842..2014
                     /locus_tag="VFY80_04070"
     CDS             842..2014
                     /locus_tag="VFY80_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6007112.1"
                     /translation="MTAPLRLDQLDTFVVALPIRRVHRWVGLDTPVGAGYVITRARLS
                     DGTVGWGEAQAIKTWGGDDWSRYGETPRTTVTIIHDFLVPALKELDVRQIEHVHAAMN
                     RTIRGYPYAKAAVEVALLDAIARGLGVPVYQLLGGRFRDTVPLAHSIGLMPIEDAVAE
                     AAQVVEEGIKTLKIKIGVGVEQDVAMVRAVRRAVGDGVNIRVDANQGYKTWREAVGAI
                     RRMTELGIAYAEQPVDGIPAMAEVSARVDVPIMADESAWTERDVVDIAAAGAAQYLSV
                     YYTKPGGLWKAKRLLTVAGAHGMQCDINGSAEMGIGNAANLHLAAAAPEISLPGTIPV
                     TSTAEVQRTRIAGRKYLDDIVKTPFAYSQGCLTVPTGPGLGIEVDEEKLARYAVNP"
BASE COUNT          393 a          663 c          745 g          335 t
ORIGIN      
        1 tggcggacgc cttgctcgag gcgggcgtgg gcaagggacg catcgcgatc gagctcgatt
       61 tcatgccggc gaaggacttt ctgcagctgc gcaagcgcct gcccgaggct acgttcgagg
      121 aatgcgcgcc gatcttcttc cgcacgcgca tgatcaagag cgacgaggaa gtgagcacgc
      181 tgcgccaggt ggggaccctg agcgagcgcg tcatccacga cgtgttcaag gaagtccgcg
      241 tcgggatgac cgagatcgac gtcgcccgcc tgatcgtcga ccagatgatc gccggcgggt
      301 cgtccaactt caagtacagg gtgggatcgg gggtgaacag ctcgatcacg aactgcggca
      361 cgacgctcaa gaagatcgag gccggcgatc tgatccgcgt ggaggtgctc ggcgagatcg
      421 acaactatcg ctccaacgtg acgcgcaccg cggtcgtcgg caagccgacc gagcgtcaga
      481 cgaatatctg gtccaccctg atgcgcgcac gcgaggtgtg caagaccatg ctcaagcccg
      541 gcacgccggt ggcggacgtg taccgcacct atgtgcgcat gtgccgggag aacgggatcg
      601 agccgacgct caagttcctc ggtcacggca tcggccagac gattcacgag gagccttatc
      661 tgacggacac gcgcgacatc gtgttcgagc ccaacgtgac gttcacgatg gagccgctct
      721 acatgattcc gggcgagatg ggctttcacg tcgaggacat gtacgtgatc acgagcgtcg
      781 ggttcgaccc gatcacgggc aatatctgca acaacgacac gctgatcgag gtgggccggg
      841 catgaccgcg ccgttgcggc tggatcagct cgatacgttc gtcgtcgcgc tgccgatccg
      901 gcgggtgcac cgctgggtcg ggctcgatac cccggtcgga gcgggctacg tgatcacgcg
      961 tgcccggttg agcgacggca cggtcggctg gggcgaagcc caggcgatca agacctgggg
     1021 cggcgatgac tggagccggt acggagagac gcccaggacc accgtcacga tcatccacga
     1081 ttttctggtc cccgcgctga aggagctcga cgtgcggcag atcgagcacg tgcatgccgc
     1141 gatgaaccgc acgatacgcg gctatccgta tgccaaggcg gcggtagaag tggcgctgct
     1201 cgatgccatc gcgcggggcc tgggggtgcc ggtgtatcaa ttgctgggcg gacgattccg
     1261 cgacaccgtg ccgctcgcgc acagcattgg cctcatgccg atcgaggacg cggtcgccga
     1321 ggccgcacag gtggtggagg aggggatcaa gaccctcaag atcaagatcg gcgtcggcgt
     1381 ggagcaggat gtcgcgatgg tgcgggccgt gcggcgtgcc gtcggcgatg gcgtgaacat
     1441 ccgcgtcgat gccaatcagg gctacaagac ctggcgcgaa gcggtgggcg ccatccggcg
     1501 catgaccgag ctcggcatcg cctatgcgga gcagccggtc gacggcatcc ccgccatggc
     1561 ggaagtctcg gcgcgcgtgg acgtgccgat catggccgac gaaagcgcat ggaccgagcg
     1621 cgacgtcgtc gacatcgctg ccgccggcgc tgcacagtat ctgtccgtct attacaccaa
     1681 gccgggcggg ctgtggaagg ccaagcggct gctcaccgtc gccggtgccc acggcatgca
     1741 atgcgacatc aacggctcgg ccgagatggg catcggcaac gcggcgaacc tgcatctggc
     1801 tgccgcagcg ccggagatca gccttcccgg aacgattccg gtcacttcga ccgcggaggt
     1861 gcagcggacc cggatcgcgg gacggaaata tctggacgac atcgtgaaga cgccgttcgc
     1921 gtattcgcaa ggatgcctga cggtgccgac cgggccgggg ctcggcatcg aagtcgacga
     1981 agagaagctc gctcgctacg ccgtgaatcc gtgacgacca gcgtcgaaca tcccggctgg
     2041 aaagcctccc gcccggtcgc agagctgccg ctgctgcccc agtgcccggt cccgttgttc
     2101 gcaggcgtgc ggttgcgcgc gcgcttcgga aggcgc
//
LOCUS       DASURG010000239         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1369863, whole genome shotgun sequence.
ACCESSION   DASURG010000239 DASURG010000000
VERSION     DASURG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369863"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFY80_04075"
     CDS             <1..297
                     /locus_tag="VFY80_04075"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HEX6007113.1"
                     /translation="QSGPICIECLELYHYAARRLERCVFGDAKPTCSHCVVHCYTRDM
                     RERVRAVMRWAGPRMLLRHPILGIAHLIDEHRPVPRLPAKPAARETRNSGSIPE"
     gene            complement(304..606)
                     /locus_tag="VFY80_04080"
     CDS             complement(304..606)
                     /locus_tag="VFY80_04080"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6007114.1"
                     /translation="MLWAIARVAGPKFTQLREKLLEPHLDYLRSQKNILVLSGGTTSD
                     DGKEFVGSLLIVNVGTRGEAQAFVDGDPFTQAGLFTDVTITRMRKGQWNPEAADGA"
     gene            749..1909
                     /locus_tag="VFY80_04085"
     CDS             749..1909
                     /locus_tag="VFY80_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6007115.1"
                     /translation="MNKPPLSPLEAARQLAPRIRASADEIEANRELPRPLFEALADAG
                     LFHLAVPRAIGGAEIDFPIYVQVLEEIGKADASTGWIVNQCATFGTYAARMAPKVARE
                     IWIDTPRSIVANTPSPNAQAVVVPGGYQVTGHQGFSTGCRHASWIAAHATVIEDGKPR
                     VRDGKPETRYFIMPVAHVQLLDTWRVRGMRGTGTHHFAVSDVFVPEERTVFLAALPLA
                     DAGPRYKIPIQLAFAGGDAAVALGMARSCLAAFYDLAGSKTPRFQNLLRDQPLTQLSV
                     GQAEATLRAGRAFLMEAIGDFWAEATSEDVVSMERRAALRAATTHAIRLAAQVIDSIY
                     TMSGATAVFEGNVIQRHFQDIHVITQHLHGRLAHYELAGQYWLGLPLDDTRL"
     gene            complement(1911..>2155)
                     /locus_tag="VFY80_04090"
     CDS             complement(1911..>2155)
                     /locus_tag="VFY80_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007116.1"
                     /translation="PGFVITQWFGFLAPAGTPRPIVDRLYQALTRGASSPDVVNRLAE
                     QGVEVVNRKPEEFAAVIKREIAQWRKVVQAAGITPR"
BASE COUNT          377 a          728 c          685 g          365 t
ORIGIN      
        1 caatcggggc cgatctgcat cgaatgcctg gagctgtacc actacgccgc gcgcaggctg
       61 gagcgttgcg tgttcggaga tgccaagccc acctgcagcc attgtgtggt gcactgttac
      121 acccgcgaca tgcgggagag agtccgcgcg gtgatgcgct gggcgggccc gcggatgctg
      181 ctccgccacc cgatcctcgg catcgcgcac ctgatcgacg agcaccggcc ggtgccgcgg
      241 ctccccgcga agcccgctgc gcgagagacc cgcaactccg gctccatccc ggagtaacgc
      301 gccttacgcg ccgtccgccg cctccggatt ccactgcccc ttgcgcatgc gggtaatggt
      361 cacgtccgta aacaggccgg cctgggtgaa cggatcgcca tccacgaacg cttgagcttc
      421 cccgcgggtc ccgacgttca cgatgagcag gctgccgacg aattccttgc cgtcgtccga
      481 cgtcgtcccg cccgacagca ccaggatgtt cttctggctc cgcaggtaat cgaggtgcgg
      541 ctccagcagc ttctccctca gttgggtaaa tttcggtccg gcaacgcgcg cgatggccca
      601 gagcatggtg gtctcctcct tcggccggat tgtgaccggt tttccactca aacggcaaag
      661 agaatgccgg ctcgcgccag gccgacgatt gtttgacggg aggcggcgga ttcgggacac
      721 tcccggacgt gccgaagaac gtgaagccgt gaataaaccg ccgctatcgc cgctcgaagc
      781 cgcgcgccaa cttgcgccgc gcatccgcgc aagcgcggac gaaatcgagg caaaccgcga
      841 attgccgcgg ccgctcttcg aagcgctcgc cgacgccggt ctgtttcatc tcgccgtgcc
      901 gcgcgcaatc gggggcgccg agatcgattt tccgatctac gtgcaggtgc tggaagagat
      961 cggcaaggcc gatgcgagca ccgggtggat cgtgaaccaa tgcgccacgt tcggcacgta
     1021 tgcggcccgc atggcgccca aagtcgcgcg cgagatctgg atcgacacgc cgcggagcat
     1081 cgtcgccaac acgccctcgc ccaacgcaca agcggtggtc gtgccgggcg gataccaggt
     1141 gacggggcat cagggcttca gcaccggctg ccggcacgcg tcctggatcg cggcgcacgc
     1201 aaccgtcatc gaagacggca agccgcgcgt cagagacgga aaaccggaga cgcgctactt
     1261 catcatgccg gtcgcccacg tgcagctgct cgatacgtgg cgcgtgcgcg gcatgcgcgg
     1321 caccggcacg catcacttcg ccgtcagcga tgtattcgtg cccgaagagc gaacggtgtt
     1381 tctcgccgcc ttgccactcg ccgatgctgg accgcgatac aagatcccga tccagctcgc
     1441 attcgcaggc ggcgacgcgg ccgttgcgct cggtatggcg cgaagttgtc tcgcggcatt
     1501 ctacgacctc gccggctcga aaacgccgcg tttccagaac ctgctgcgcg atcagccgct
     1561 cacgcagttg agcgtcggcc aggcggaagc cacgctgcgc gccggtcgcg cgtttctgat
     1621 ggaagccatc ggagatttct gggctgaagc gacctcagaa gatgtggtga gcatggagcg
     1681 gcgcgcggcc ctgcgcgctg cgacaacgca tgctatccgg cttgccgctc aggtgatcga
     1741 cagcatctat acgatgtcgg gcgcgaccgc cgtattcgag ggcaatgtga tccagcgcca
     1801 cttccaggac atccacgtta tcacgcagca cctgcacggg cgccttgcgc actacgagct
     1861 cgccgggcag tactggttgg ggctaccgct cgacgatacg cggctctagt tcaacgcggg
     1921 gtgatgcctg ccgcctggac gacttttctc cactgcgcga tttcgcgctt gatgacggct
     1981 gcaaattcct cgggcttgcg gttgacgacc tccaccccct gctccgccag ccggtttacg
     2041 acatccggac tgctcgcacc gcgcgtgagc gcctgataca ggcgatcgac gatagggcgc
     2101 ggtgtgccgg ccggcgcgag aaaaccaaac cattgcgtga tgacgaagcc cggca
//
LOCUS       DASURG010000240         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1165605, whole genome shotgun sequence.
ACCESSION   DASURG010000240 DASURG010000000
VERSION     DASURG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165605"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1336
                     /locus_tag="VFY80_04095"
     CDS             155..1336
                     /locus_tag="VFY80_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007117.1"
                     /translation="MATKSENNRVAGMSAAEAAARILAVDDDQRNLLALQELLQAPGQ
                     ELVLARSGEEALQHVEREEFAVILLDARMPGMDGFATARRIRERPGQNTPIIFLTAAY
                     EDLGSIFRGYEVGAVDYIVKPLIPEVLKSKISVFVDLYKKSVALKREIAERRVTEERL
                     RESEENLRALAARLQSVREEEQIRIAREIHDELGQALTGLKMDLTWLAGGLKPDQQPL
                     VEKTSAMFRLIDGTIRSVRRIASGLRPEVLDEIGLAAAISWQARDFQVRSGIRCNVEL
                     PPDGPALDSARSTAMFRIFQELLTNVARHAHATRVDVRMGVDGHTMWLTVQDNGRGIS
                     EQKIMSSKSLGFLGMRERVLPFDGRIDIRGQRGKGTMVTIRIPLETAPASARVEQGER
                     G"
     gene            1380..2009
                     /locus_tag="VFY80_04100"
     CDS             1380..2009
                     /locus_tag="VFY80_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6007118.1"
                     /translation="MKVLIADDHPVVRHGLRQILATDSTVEVVGEAKNGTETLDLARK
                     VAWDVAIVDYSMPGRSGLDLLKELKREFPQRPVLILSMYPEELHATRVLKAGGAGYIN
                     KECVGEELTSALKKVVNGGRYVSRSLAEKLAFELARDTQKPLHETLSDREYRVMWLLA
                     SGKQIHQIASELFVSPSTISTYRARIFRKLRLRTNAELVHYAIKHRLVD"
     gene            2248..>2634
                     /locus_tag="VFY80_04105"
     CDS             2248..>2634
                     /locus_tag="VFY80_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6007119.1"
                     /translation="MKILAIVLAGGEGTRLYPLTAENPKPAIPFVNGYRIIDFVLSNL
                     VNSRIAPIYVVGQYKPQSLVRHIETAWDRWSKGPEAKIRIVLPGANGSQSQFRGTADA
                     VYQNLRLIAHHRPDIVAVFAADHVYRM"
BASE COUNT          546 a          779 c          839 g          470 t
ORIGIN      
        1 cggtagcgaa tatccgacat tccccattaa tgcgcaatcg ggggctttag tccgatgaga
       61 ctaagtcttg gaccgtcagg caaagcgcgt aaaaaaagca gcgcgacggt ggggttggct
      121 tgagtggggg ttgccgcgat aataccgcga cgcaatggca acaaagagcg agaacaacag
      181 ggtggcgggg atgtctgcgg ccgaagcggc ggccagaata cttgcggtcg acgacgatca
      241 gcgcaatcta ctggcgctgc aggagctctt gcaggcgccg ggacaggaac tcgtgctggc
      301 gcgctccggc gaggaggcgc tgcagcacgt cgaacgggag gaattcgcgg ttatcctgct
      361 cgatgcgcgc atgcccggga tggatggttt cgctaccgcc cgccgcattc gtgagcggcc
      421 cggacagaat acaccgatca tctttctcac ggcggcgtac gaggacctgg gctcgatctt
      481 ccgcggctat gaagtgggtg cggtggacta cattgtgaag ccgctcattc ccgaggtact
      541 gaaatccaag atctcggtct tcgtcgacct ctataagaaa agtgtcgcgc tgaagcgaga
      601 aatcgccgag cgccgcgtta ccgaggagcg gctgcgggaa tcggaagaaa acctgcgcgc
      661 gctggccgcc cgcctgcaat cggtgcgcga ggaggagcag atccgcatcg cgcgcgagat
      721 tcacgatgag ctgggtcagg cgctcaccgg gctcaagatg gacctgacct ggctcgctgg
      781 ggggctcaag cccgaccagc agcccttggt cgagaaaacc agcgcgatgt tccgcctgat
      841 cgacggcacg attcgatcgg tacggcgtat cgcttcgggg ttacgccccg aggtgctgga
      901 cgaaatcggg ctcgccgccg cgatcagctg gcaggcgcgg gattttcagg tccgttcagg
      961 catacggtgc aacgtggagc tgccaccgga cggaccggcg ctcgattcgg cgcgctcgac
     1021 agccatgttc cggattttcc aggagctgct gaccaacgtg gcgcgccacg cccacgcgac
     1081 ccgagtcgac gtccgaatgg gcgtggatgg gcacaccatg tggctgaccg tgcaggacaa
     1141 tggcagaggg atcagcgagc agaagatcat gagttcgaag tcgctcggct ttctcggcat
     1201 gcgggaaagg gtattgccgt tcgacggcag gatcgatatc cgtggccagc gcggcaaggg
     1261 caccatggtg acgatccgca ttccgctcga gaccgcccca gcatcggccc gggtggagca
     1321 gggagagcgg ggatgagcgc cgcgcaggcg ccgcgctcgc agagaaaaag gaaaacagca
     1381 tgaaagtact gattgcggac gaccatcccg tggtgcgtca cggccttcgg cagattctcg
     1441 ccaccgattc gaccgtcgag gtggtgggag aagccaagaa cggcaccgag actctggatc
     1501 tcgcccgcaa ggtcgcctgg gacgtcgcca ttgtcgatta ttccatgccc ggtcgcagcg
     1561 gtctggatct cctgaaggag ctgaaacggg agtttcccca gcgtccggtc ctcattctga
     1621 gcatgtaccc ggaagaactg catgcaaccc gcgttctcaa ggcgggcggc gccgggtaca
     1681 tcaacaagga gtgcgttggc gaagaactca cttcggcgct caaaaaagtg gtgaacggtg
     1741 ggcgctatgt cagtcgctcg cttgccgaga agctcgcctt cgagcttgcg cgggataccc
     1801 agaagccatt gcatgaaacc ctctccgacc gcgagtaccg ggtgatgtgg ctgctcgcgt
     1861 ccgggaagca gattcaccag atcgcttccg agcttttcgt aagcccgagc accatcagca
     1921 cttaccgagc caggatcttt cgcaagctgc ggttgcgcac caacgccgaa ctcgtgcact
     1981 acgcaatcaa gcaccggctg gtagactaga gccgcttcta cttgcggctc caccttcagc
     2041 gcctttcccg cgctttctgc cgtgtcgcgc cgggacgaca cggcgacagt acatcacgac
     2101 aaaaatttcc agcttcaccg atagcgcgtg acgctcgcga aaactatatt cgatcggcga
     2161 cctgcgctcc tttcggagtg atgagaacga tgcaatcgca ggcgacgcgc tttcgaatgg
     2221 ctaaaaagct ccaaacgggg aacccacatg aaaattcttg cgatcgtcct ggccggcggt
     2281 gaaggcacgc ggctttaccc cctgacggct gaaaacccga agcctgccat accgttcgtg
     2341 aacggatatc gcatcatcga tttcgtgctg agcaacctcg tcaactcgcg catcgcgccg
     2401 atctacgtgg tgggtcagta caagccgcag tcgcttgtgc gccatatcga gacggcgtgg
     2461 gatcgctggt ccaagggccc ggaagcgaag attcgaatcg tgctcccggg cgcgaacggg
     2521 agtcagtcgc aattcagggg cacggcggat gcggtctatc agaacttgcg cctcatcgcg
     2581 caccaccggc ccgatatcgt cgcggttttc gccgcggatc acgtgtaccg catg
//
LOCUS       DASURG010000241         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_858670, whole genome shotgun sequence.
ACCESSION   DASURG010000241 DASURG010000000
VERSION     DASURG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858670"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFY80_04110"
     CDS             complement(<1..288)
                     /locus_tag="VFY80_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007120.1"
                     /translation="MTTRELEGKVALVTGASRHIGRAIALALAQGGAAVAVNARTTRE
                     DAEKVATEIRASGGQSEVCMADIADAAQVGAMVGAVLERFRRLDILVLNASV"
     gene            477..851
                     /gene="hfq"
                     /locus_tag="VFY80_04115"
     CDS             477..851
                     /gene="hfq"
                     /locus_tag="VFY80_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX6007121.1"
                     /translation="MAARLLRPIKKKPSAPGTQKPQKGSHATGQAAQDRFLRQMIDEH
                     RKVAVFLASGVKLEGEIVSYDQYVILMKGALTDSVYKHAVSTIQPIDAGSTSAKPPRK
                     TETRAPTIVRRSKPRLVKVGGN"
     gene            891..1727
                     /locus_tag="VFY80_04120"
     CDS             891..1727
                     /locus_tag="VFY80_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6007122.1"
                     /translation="MRTELVSIPTETVPLEGAYYEPESAPSAGAVLLFHGNTMNFYVG
                     APRFLPPVLTRLGFACLAFNRRGHDILSTRASRMAEGGAYQFTHEALADNRSAALWLS
                     ARGFRHPVVIGHSNGGMLGVQHVVDHPQTPALVLLSAGRGGPRGAVATGTEKLFAMDR
                     LDQLTAQARCLVAEGRGRELMFMPGWWYVISAESFLDRLDTVPDTIALAPRITCPVLA
                     IRGDKEDVERYPAEEFQRAAGGPCTVEIVRDCDHFYNGREDTVAGIVATWLAQTLRLP
                     PP"
     gene            complement(1734..2858)
                     /locus_tag="VFY80_04125"
     CDS             complement(1734..2858)
                     /locus_tag="VFY80_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6007123.1"
                     /translation="MKIAKVTCWLLRIPFTFPLVKETQHALANFVEVESDDGIKGHAL
                     STYPMPHGVREFINREAAPAINGMDALRPEEIRNKLFLATARKHVMGAWNCAVSLIDI
                     ALWDVKGKALGQPVWKLLGGAHAKLPAYRTFGLPRYSIEELIEVARMLVSEGQNSLKM
                     VVAKGSNPYDEILGQPTDADILEDARRVAALREAVGPDIELMMDANKGATFAQALKLA
                     KLCEPYNLTWFEDPVLKGDPRLMARLRAQTTIPIAAGSTATSDLMFLREYLVQEAVDY
                     LQPNVRDIGGYTQGLKAAGIAQAFNVPLAMGGNYPHINMHLHAGVPNGGRVEFHWQGW
                     KCVEALFEGAPAPVRGTVTLPAAPGLGFTPKSGILDLAVD"
     gene            2973..3146
                     /locus_tag="VFY80_04130"
     CDS             2973..3146
                     /locus_tag="VFY80_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX6007124.1"
                     /translation="MPAFMCGRYVSPDEAAIERTWHIGRGNMPFVPRYNIAPTEMVPV
                     LTRDRDTSALQLA"
     gene            3146..>3310
                     /locus_tag="VFY80_04135"
     CDS             3146..>3310
                     /locus_tag="VFY80_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6007125.1"
                     /translation="MRSDTDREILIVGAGAAGLAAAAEIARRGGSAHVLEARDRIGGR
                     CRSRHIPGVPV"
BASE COUNT          555 a         1114 c         1051 g          590 t
ORIGIN      
        1 caccgacgcg ttgagcacga ggatgtcgag gcgacggaag cgctcgagca ccgcgccaac
       61 cattgcgccg acctgtgctg catccgcgat gtccgccatg cacacttcag actgcccgcc
      121 cgaggcgcga atctcggtgg cgactttttc cgcgtcttcc cgcgtggtgc gcgcattcac
      181 ggccaccgcg gccccgcctt gcgcgagcgc gagcgcgatg gcgcggccga tgtgacgcga
      241 agcacccgtc acgagggcga ccttgccttc gagttcgcgt gtcgtcatgt cgtcctcctg
      301 tggcgagatc ggcagcatag cggattgtcc tgcggcggga aacgcgaaag ctgacacgtg
      361 ccgtaagacg gacgccacag ttccgcggga ggcccctcgg gttcgctgga acgtctcacg
      421 cgcgatataa tcccaatttt tattccgggc gcatcgcccg atcggagtct cgcgaaatgg
      481 ctgccaggct gctcaggccg atcaagaaga aaccttccgc tcccgggaca caaaagccgc
      541 agaaaggaag ccatgcgacc ggacaggcgg cgcaggaccg ctttctccgc cagatgatcg
      601 atgaacaccg caaggttgcg gtgtttctcg cgagcggcgt caagctcgaa ggggaaatcg
      661 tgtcctacga tcagtatgtc attctgatga aaggcgcgct caccgacagc gtgtacaagc
      721 acgccgtttc caccatccag cctatcgatg cgggcagcac cagcgccaag ccgccgcgga
      781 aaaccgaaac ccgcgcaccg accatcgtga ggcgcagcaa gccgcggctc gtcaaggtcg
      841 gcggcaactg agtctctcgt aaagcctggt ctcgccgcct gcgggagcgc atgcgcaccg
      901 agctcgtctc gattcccacc gaaacggttc ctctggaggg cgcgtactac gagcctgaat
      961 cggcgccaag cgccggcgcc gtgctgctct ttcatggcaa cacgatgaac ttctatgtcg
     1021 gcgcgccgcg tttcctgccg ccggtgctga cgcgcctggg ctttgcttgc ctcgcgttca
     1081 atcgccgcgg gcatgacatc ctgagcaccc gcgcgagccg catggccgaa ggcggggcct
     1141 atcagtttac gcacgaagcg ctcgcggaca atcgcagcgc cgcactgtgg ctctccgcac
     1201 gcggctttcg gcatcccgtc gtcattggcc acagcaacgg cggcatgctg ggcgtccagc
     1261 acgtcgtgga tcatccgcaa acgccggcgc tcgtgttgct ctcggccgga cggggcgggc
     1321 ctcgtggagc cgtcgcgacc ggaaccgaaa agcttttcgc gatggaccgg ctcgatcagc
     1381 tgacggcgca ggcacgctgc ctcgtggcgg aaggccgcgg gcgcgaactc atgttcatgc
     1441 cggggtggtg gtacgtgatc agcgcggaga gctttctcga tcgcctcgac accgtgcccg
     1501 acaccatcgc gctcgcgccg aggatcacgt gtccggtgct ggccatacgc ggcgacaagg
     1561 aggacgtcga gcgctacccc gctgaggaat ttcagcgcgc ggcgggcggc ccgtgcaccg
     1621 tcgagatcgt tcgcgactgc gatcacttct acaacggccg cgaggacacc gtggccggca
     1681 tcgttgcgac ctggctcgcg cagacgctga gattgccgcc gccctgattc cggtcaatcc
     1741 acggcgagat cgagtattcc gctcttcggc gtaaatccga ggcccggagc ggccggcagc
     1801 gtgaccgttc ccctgaccgg ggccggcgcg ccctcgaaga gcgcctcgac acatttccag
     1861 ccctgccagt ggaattcgac gcggccgcca ttcggcacgc ccgcatggag atgcatgttg
     1921 atgtgcgggt aattgccgcc catcgcgagc ggcacgttga acgcctgcgc gatgccggct
     1981 gctttgagac cctgcgtgta gccgccgatg tcgcgaacgt tcggctggag atagtccacc
     2041 gcctcctgca cgaggtactc gcgcaggaac atgaggtcgg atgtcgcggt gctgcccgca
     2101 gcgatcggga tcgtcgtttg tgcgcgtagc cgcgccatca gccgcggatc gcccttcaat
     2161 accggatcct cgaaccacgt gaggttgtac ggctcgcaca gcttcgcaag cttgagcgcc
     2221 tgtgcgaatg tcgcaccctt gttcgcgtcc atcatgagct cgatgtcggg ccccaccgcc
     2281 tcgcgcagtg ccgccacgcg acgggcgtct tccaggatgt ccgcgtcagt gggttgtccc
     2341 aggatttcgt cgtagggatt cgatcccttg gcgacgacca tcttgaggct gttctggccc
     2401 tcggagacga gcatgcgcgc gacttcgatc agctcctcga tgctgtagcg cggcaggccg
     2461 aacgttctgt aggcgggcag cttcgcgtgc gcgccgccca gtagcttcca caccggttgc
     2521 ccgagcgcct tgcctttgac atcccagagc gcgatgtcga tcaggctgac cgcgcagttc
     2581 cacgcgccca tcacgtgctt gcgcgcggtc gcgagaaaga gcttgttgcg gatttcctcg
     2641 ggccgcaggg catccatgcc gttgatcgcc ggcgcggcct cgcgattgat gaactcgcgc
     2701 actccgtgcg gcatcggata agtcgaaagc gcgtgtccct tgataccgtc gtcgctttcc
     2761 acctcgacga aattcgcgag cgcgtgctgg gtctccttca ccagcggaaa cgtgaacggg
     2821 atgcgcagca gccagcaggt cacttttgcg atcttcatgg cctcctcgat tggcaatgca
     2881 gtgcggccgc atatcatagc ctacggatcc cggacatgct ggcgcattta cagtgtcgcc
     2941 ggcaacgcgt aaggttcgct ccgtcatgcg ctatgcctgc cttcatgtgc ggtcgctatg
     3001 taagcccgga tgaggcggcg atcgagcgca cctggcacat cggtcgcggc aacatgccgt
     3061 tcgtcccgcg ctacaacatc gcccccaccg agatggtgcc ggtgctgacg cgcgatcgcg
     3121 acaccagcgc gctccaactc gcatgatgcg ctcggatacg gatcgcgaga tcctgatcgt
     3181 cggagccggc gccgccggcc tcgctgccgc agctgaaatc gcccggcgag gcggctcggc
     3241 gcacgtcctc gaggcacgcg accgcatcgg cgggcgctgc cgctcgcgcc acataccggg
     3301 cgtgccggtc
//
LOCUS       DASURG010000242         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_42825, whole genome shotgun sequence.
ACCESSION   DASURG010000242 DASURG010000000
VERSION     DASURG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42825"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFY80_04140"
     CDS             <1..284
                     /locus_tag="VFY80_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007126.1"
                     /translation="PTLPDIPSLSEFLPGYDASLWTGLLAPKGTPRAAIDRIQRETTK
                     LLQSADVNAAFQRLGADVVATDPKAFGKFLEQEYAKWGKTIRDLKLVVN"
     gene            complement(281..1156)
                     /locus_tag="VFY80_04145"
     CDS             complement(281..1156)
                     /locus_tag="VFY80_04145"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX6007127.1"
                     /translation="MDVNLMGPGFCAEIRDVGLAEVAEHDAAYAEVRAAFETYSVLLF
                     RRQSITDALQVAFSRRFGPLEIAEAASLGEGTPFSILTNIDRATGALVPPDHKEALRA
                     RANQLWHTDSCFKTPPALASVLSARVIPPHGGETEFASTRRAWARLSPARRAQLADAY
                     AWHDYAHSRGKIAAHLASEREKAALPPVSWRVRWRNPANGLDALYLASHTFAIEGMPQ
                     DAAQSLIAELIDFATAPGETYLHRWQPGDVIMWDNRAVLHRGRPWPGDQPRHMVRTTI
                     SATEADGLGAMRPAA"
     gene            1284..>2063
                     /locus_tag="VFY80_04150"
     CDS             1284..>2063
                     /locus_tag="VFY80_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6007128.1"
                     /translation="MPLKDERNTDSLRSMMVRFANWARARWLSLLVLAAILGAFPVAG
                     LSLLAHKERELIFRIEPGVARWYSGLPAGVQELELPVSAEGGVLHAWWWPSGSRNAPA
                     VLYLHGSRWNLTGQLYRIEQLREFGFSVLAIDYRGFGRSRGGPPSESSVYEDARVAWE
                     RLVQLQPDTAKRYIYGHSLGGAVAVELAHFLAVKADGSRKGAAGLIVESSFTSLADVA
                     AAITNVRLPLHWLITQKFQSIDKIDEVGVPVLVVHGTADRYI"
BASE COUNT          349 a          649 c          685 g          380 t
ORIGIN      
        1 cgccgacgct gccggacatt ccttccctgt cggagtttct ccccggctac gatgcttcgc
       61 tgtggaccgg gctgctcgcg ccgaaaggca cgccgcgggc ggcgatcgat cgcatccagc
      121 gcgaaacgac aaaactgctt cagtcagcgg acgtgaatgc cgcgtttcag cggttgggcg
      181 cggatgtcgt cgccacggat ccgaaggcct ttggcaaatt cctggagcag gaatacgcca
      241 agtggggcaa gacgatccgc gatctgaagc tcgtcgtcaa ctaggccgcg ggccgcatcg
      301 caccgaggcc gtctgcctcg gtcgcggaga tcgtcgtgcg caccatgtgg cgcggttgat
      361 cgccgggcca tggacggccg cggtggagca cggcacggtt gtcccacatg atcacgtcgc
      421 ccggctgcca gcggtgcaga taggtttcgc cgggcgccgt cgcgaagtcg atcaactcgg
      481 caatcagtga ctgagctgcg tcctgcggca tcccctcgat ggcaaacgta tgggatgcga
      541 gatagagcgc atcgagcccg tttgcggggt tgcgccagcg gacgcgccac gagacgggcg
      601 gcaacgccgc tttttcgcgc tcggacgcga ggtgcgctgc gatcttgccg cgggagtgcg
      661 cataatcgtg ccaggcatag gcatcggcaa gctgcgcgcg ccgcgcgggt gaaagtcgcg
      721 cccacgcgcg gcgtgtcgag gcgaattccg tttcaccgcc atgcggcggg atgacgcgcg
      781 ccgagagcac cgaagcgagt gcaggcggcg tcttgaagca gctgtccgta tgccacagct
      841 ggttggcgcg cgcacgcagc gcttccttgt ggtccggcgg gacgagtgcc cctgtcgccc
      901 gatcgatgtt ggtcaggatg ctgaacggcg tgccttcgcc gagcgatgcc gcttctgcaa
      961 tctcgagcgg accgaaacgc cgcgagaacg cgacctgcaa cgcgtccgtg atcgactgac
     1021 ggcggaagag cagcaccgaa taggtctcga acgccgcgcg cacttcggcg taggcggcgt
     1081 cgtgctccgc aacttcagcc agccctacgt cacggatttc cgcacagaat ccgggtccca
     1141 tcagattgac gtccatcagt gcctcggttg aatggaacaa aagaactgag tcgcgctcaa
     1201 aatgctcaac ggcgaccggt cgccagccgc gatatgctat caccctcgag cgattagagc
     1261 gggcagtaca cctaccgccc tgaatgccgt taaaggatga gcgaaacacc gattcactgc
     1321 gctcgatgat ggtacgtttt gcgaactggg ctcgcgcacg ctggttgagt ctcctcgtcc
     1381 ttgcggcgat actgggcgca tttcccgtcg cgggattgtc gctgctcgca cacaaggagc
     1441 gcgaactcat cttccgcatc gagcccggcg tcgcgcgctg gtacagcggc ctgcctgccg
     1501 gcgtgcagga gctggagctg ccggtttccg cggagggcgg cgtgctgcat gcctggtggt
     1561 ggccctcggg atcgcgcaac gcgcccgcgg tgctctatct gcacggctcc cgctggaatc
     1621 tcacaggaca gctctatcgc atcgagcagc tacgcgaatt cggattttcg gtgcttgcga
     1681 tcgactatcg cggctttggc cggagtcggg gcggaccgcc ctccgagtcg agcgtgtatg
     1741 aggacgcacg cgtcgcgtgg gagcggctcg ttcagctcca gcccgatacc gcgaagcgtt
     1801 acatctatgg gcactctctg ggcggcgcgg ttgcagtcga gcttgcgcac tttcttgccg
     1861 taaaggccga tgggtcgcgc aagggcgccg ctgggctgat cgtcgaatct tcgttcactt
     1921 ctctcgccga cgttgccgcc gcgattacca acgtccggct gccgctgcac tggctgatta
     1981 cccagaagtt ccagtcgatc gacaagatcg acgaggtcgg ggtcccggtg ctggtggtgc
     2041 acggcacggc ggatcgctat att
//
LOCUS       DASURG010000243         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_247564, whole genome shotgun sequence.
ACCESSION   DASURG010000243 DASURG010000000
VERSION     DASURG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247564"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFY80_04155"
     CDS             complement(<1..605)
                     /locus_tag="VFY80_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6007129.1"
                     /translation="MHDHELVFLSIARQAELIRERALSPVELVRAYLGRIARYDGVLR
                     AYITVRAEEALAAAADAEREIAAGRYRGPLHGIPFGAKDQLCTRGIRTTVGSKVMSDY
                     VPDFDATVIARLAQAGAILIGKENLHEFGKGGTNVFPYGQPRNPWDPARNPGSSSSGS
                     GIAPAAGLCSASLGEDTGGSVRGPAAACGAVGLRPTFGRVSR"
     gene            711..1697
                     /locus_tag="VFY80_04160"
     CDS             711..1697
                     /locus_tag="VFY80_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007130.1"
                     /translation="MTFVSRVHVSIGALSGVLMLCASSVDAQTAWKPERTVEVVVFAA
                     PGGGNDKSARVMHKIWQETKLLDAVVTNKVGGGGSLAYTYVSQKTGDAHHIAIAQAGL
                     NTNHITGRSPIHYSDVTPLAFVGSEPVALAVRADSPYRTLKDFAEKLKKDPAALSISV
                     GSTRGAINHFTIARLAKAAGVDPRQLKILVFGGGAESVTNLLGGHIDAMVQAVNNAIP
                     HHKAGKMRILGLSTPKRSPGIPDAPTFREQGYDVVMEGWTIFVGPKGTTPAQVAYWEG
                     IFQKTVNQDLWKQYLESNSWEWGYKNSQETLAYLKKDYEQSKALLTELGLAK"
     gene            1694..>2233
                     /locus_tag="VFY80_04165"
     CDS             1694..>2233
                     /locus_tag="VFY80_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6007131.1"
                     /translation="MNPLSASEAAAAIAAGKLSSQALVEACLERIAQREDEVRAWAHI
                     DPAQALEQARQRDRERPRSRLHGIPVGVKDVIDTCDMPTEYGSPIYKGHRPACDAACV
                     AQVRELGGVILGKTVSTEFATRHPNKTRNPRNLAHTPGGSSSGSAAAVADGMVPIAFG
                     TQTSSSIVRPAAFCGVVGYK"
BASE COUNT          417 a          722 c          726 g          368 t
ORIGIN      
        1 cggctgacgc gcccgaacgt cggccgcaat ccgaccgcgc cgcatgcggc ggccggcccc
       61 ctgaccgatc cgccggtatc ttcgccgagc gaggcggaac acaatcccgc cgccggtgcg
      121 atgcccgatc cgctcgacga acttccggga ttgcgcgcgg ggtcccacgg gttgcgcggc
      181 tgcccgtaag ggaagacatt ggtgccgccc ttgccgaatt cgtgaagatt ctccttgccg
      241 atcaggatcg cgccggcctg cgcgagacgc gcaatcaccg ttgcgtcgaa atccggcacg
      301 tagtcgctca tgaccttcga gccgacggtg gtgcgtatgc cgcgcgtgca aagctggtcc
      361 tttgcaccga acggaatgcc gtgcagcgga ccgcgatatc ggcctgcagc aatttcgcgc
      421 tcggcgtcgg ccgctgcggc gagcgcctct tcggcacgta cggtgatgta ggcgcgcagc
      481 acgccgtcat agcgcgcgat acgcccgagg taggcacgga cgagctcaac gggagaaagc
      541 gcgcgctcgc gtatgagttc agcctgccgg gcgatgctca ggaaaacgag ttcgtggtcg
      601 tgcatgatgg tggaacgttg ccgcgggatt ctagcacgat ggatcgccgt tctttgcggc
      661 cggatgcgtg cgcgctaaca tagcggcgat tccctcaccg gcgagccccc atgactttcg
      721 tatcccgcgt ccatgtttcc attggagccc tttccggcgt gctgatgctg tgcgcctcgt
      781 ccgtagatgc gcagacggca tggaagcccg agcgcaccgt ggaagtggtc gtgttcgcag
      841 cgcctggcgg cggcaacgac aagtctgcgc gggtgatgca caagatctgg caggaaacca
      901 aacttctcga cgcggtggtc acgaacaagg tgggcggcgg cggttcgctc gcctatacct
      961 acgtgagcca gaagacgggc gatgcgcatc acatcgcgat cgcgcaggcc ggcctcaaca
     1021 ccaaccacat caccggcaga agtccgattc actattccga cgtgacgccg ctcgcctttg
     1081 tggggagcga gccggttgcg ctcgcggtgc gcgccgactc gccgtaccgg acgctgaagg
     1141 acttcgcgga gaaactgaag aaggaccctg cggcgctctc gatctctgtc ggcagcacgc
     1201 gcggcgcgat caaccatttc acgatcgcgc ggctcgcgaa ggctgcgggc gtcgatccgc
     1261 gccaactgaa gatcctggta ttcggcggcg gcgcggaatc ggtgaccaac cttctcggcg
     1321 ggcacatcga cgccatggta caggccgtca acaatgcgat tccacaccat aaagcgggca
     1381 agatgcgcat tctcggcctc tcgacgccga aacgctcccc cggaattccg gacgcgccga
     1441 cgttccgcga gcagggttac gacgtggtga tggagggctg gaccatattc gtcggaccga
     1501 aaggcacgac gcccgcccag gtcgcgtatt gggaaggaat cttccagaag acggtgaatc
     1561 aggacctgtg gaagcagtat ctcgaatcga attcctggga atggggctac aagaattcac
     1621 aggagacgct cgcgtatctc aaaaaggatt atgagcaatc gaaggcgctc ctgaccgagc
     1681 tcgggctcgc gaaatgaatc cgctttccgc atccgaagcc gccgccgcga tcgcagcggg
     1741 caagctcagt tcgcaagcgc tcgtcgaggc gtgcctcgag cgcatcgcgc agcgcgagga
     1801 cgaggtgcgc gcgtgggccc acatcgaccc tgcacaggcg ctcgagcagg cacgtcagcg
     1861 cgaccgcgaa cggccgcgca gccgcctgca cggcatacca gtcggcgtaa aagacgtgat
     1921 cgacacgtgt gacatgccga ccgaatacgg ttcgccgatc tacaagggcc accggcccgc
     1981 gtgcgatgcg gcgtgcgtcg cgcaagtacg ggaactgggc ggcgtcatcc tcggcaagac
     2041 ggtctccacc gagtttgcca cgcgccaccc caacaagacg cgtaatccgc gcaatctcgc
     2101 gcatacgccg ggcggttcct cgagcgggtc ggccgccgcg gtcgccgacg gtatggtccc
     2161 catcgcattc ggcacgcaga cctcgtcatc gatcgtccgt ccggcggcgt tttgcggggt
     2221 cgtcggttac aag
//
LOCUS       DASURG010000244         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1268079, whole genome shotgun sequence.
ACCESSION   DASURG010000244 DASURG010000000
VERSION     DASURG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268079"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFY80_04170"
     CDS             complement(<1..1036)
                     /locus_tag="VFY80_04170"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX6007132.1"
                     /translation="MLRIAVIGGSLGGLTAALLLRDAGHDVSVFERSPVALTGFGAGI
                     VAHEVSLRYFVERRARDPDTFTVAVETYRLLDQSGARLHEERVAYRFVSWGTLHRALL
                     AEFGTERYFQGIALETFDADESGVEVRFADGRAARFDLLVLADGIQSTGRARLFPAAE
                     PSYSGYVAWRGTVDERAIPRTALARLGNTLTYTLIPASHILVYPIPGADGAVAPGRRL
                     WNFVWYRNVEAGAALDTLLTDRDGVRHGVSLRPGRVQGRHVDELKRFAGANLPQEIAE
                     IVAAAEAPFIQVVMDVACPRMAVGRVCIMGDAAFTARPHAAAGTAKAAENAWTLAEAL
                     VLGGDDWQAAL"
     gene            complement(1039..1770)
                     /locus_tag="VFY80_04175"
     CDS             complement(1039..1770)
                     /locus_tag="VFY80_04175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6007133.1"
                     /translation="MQRKDIRIRSRDGGEFDCYLVTPDDTGPVPAIVLASAVHGVDPD
                     IRALADEFAGRGYIAAAPDLFWRTIPGPLGRDDDRTKVRSQPRLQRIKEGEADMADTL
                     SEVRKLEQFNGRAAAMGFCYGGPYAILGPKRLGYAAGISCHGTQMLDYIHELEGVTHP
                     VCIIWGDRDHQAPPPVLEAYRAVPARMKNVEVHIFPGVEHGYMMRGNPKAFSQPTYEF
                     SMERALSILAGLSGEGGREPLRKAS"
     gene            1909..3090
                     /locus_tag="VFY80_04180"
     CDS             1909..3090
                     /locus_tag="VFY80_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6007134.1"
                     /translation="MDFSLNEAQQTWQRRAREFAREEIRSISLERDQMEGGLAPWDWG
                     IMKKGSALGFRTLAVPRDWGGPGADFVTQGIVMAELARGDSAISKAFSQNWKWSHLIA
                     ENCTNDQKERFLKPFLADDTFVMGAASTEPNAGSDNRYPPEDDPKAGWRLRAERRGDE
                     WLLNGEKTFIANGSIGKLFFVTARTNPDVPMRDGTTVFMVPLGTPGFRVGKIFNKSGW
                     RFYQNAELVFENARVPHANIVGEVNGGVKVRSGASAEFNDFELAANALGVCDAACEQA
                     MALARKARRGGKRLYDHQLIQLRINEMHLLTEALRSYVMRTAWEMDQRRQGANAVLVM
                     NYSTDVIQRVTRLNLEIHRATDGVMDAMAHKLVRDAIIWSHLAGDTSQRLKAMRRLLA
                     H"
BASE COUNT          576 a         1065 c         1055 g          528 t
ORIGIN      
        1 cgagcgcggc ttgccagtcg tcgcctccga ggacgagcgc ctccgcgagc gtccacgcgt
       61 tctctgccgc tttcgcggta ccggccgcgg catgagggcg cgctgtaaat gcggcatcgc
      121 ccataatgca cacgcgtccg acggccatgc gcggacacgc cacgtccatg acgacttgaa
      181 tgaacggcgc ttcggccgca gcgacgattt cggcgatctc ctgcggcagg ttcgcgccgg
      241 caaagcgctt cagctcatcg acgtggcgtc cttggacccg cccgggtcgc agcgacacgc
      301 cgtgccgcac gccatcgcga tcggtgagca gcgtatcgag cgcggcaccg gcttccacgt
      361 tgcggtacca gacaaagttc cagagccgcc gccccggggc gacggcgccg tccgcgcccg
      421 gtatggggta gacgaggatg tggctcgccg ggatgagggt gtaggtcagc gtgttgccga
      481 ggcgcgcgag tgctgtccgc ggaatggcgc gctcatccac cgtgccgcgc caggccacgt
      541 agccggaata gctcggctcc gctgcaggaa aaagccgcgc gcgcccggtg gactgaatgc
      601 cgtcggcaag caccaacaga tcgaaccgtg cggcgcgccc gtccgcgaaa cggacttcga
      661 cgccggattc gtcggcgtcg aaggtctcaa gcgcaatacc ctggaaatag cgttccgtcc
      721 cgaattcggc gagaagcgcg cgatgcagcg taccccacga cacgaagcga taggcaacac
      781 gctcttcatg caggcgcgca ccgctctggt cgagcagccg ataggtctcc accgccacgg
      841 tgaaggtgtc tggatcgcgc gccctgcgct caacgaagta acgcaggctc acttcatgcg
      901 cgacgatgcc ggcaccaaag cccgtcaatg ccacgggcga gcgctcgaaa accgatacgt
      961 cgtgcccggc gtcgcgcagc agcagcgccg cggtcaggcc gcccagcgag ccgccgatga
     1021 cggcgatgcg cagcaacgct acgaggcttt gcgcagcggc tcgcgcccgc cctcgccgct
     1081 cagccctgcc aggatcgaga gcgcacgctc catcgagaac tcgtaggtcg gctggctgaa
     1141 cgctttcggg ttcccgcgca tcatgtagcc gtgctcgacg ccgggaaaga tgtgcacttc
     1201 gacgtttttc atccgcgcgg gcacggcacg atacgcttcc agcacggggg gcggcgcctg
     1261 gtgatcgcgg tccccccaga tgatgcacac cgggtgggtg acgccttcga gctcgtggat
     1321 gtagtcgagc atctgggtgc cgtgacacga aatgccggcg gcatagccca gccgcttcgg
     1381 tccgagtatc gcgtacgggc cgccgtagca gaaccccatg gccgccgcgc gaccgttgaa
     1441 ctgctcgagc ttgcggacct cactcagcgt gtccgccata tcggcttcac cctccttgat
     1501 cctctgcagc cgcggctgtg agcgaacctt ggtccggtcg tcgtcccgtc ccaaggggcc
     1561 ggggatcgtc cgccagaata gatccggcgc ggccgcaatg tagccgcgcc ctgcaaattc
     1621 atcggcgagc gcgcggatgt ccgggtccac gccgtgcacc gcggaggcga gcacgatcgc
     1681 cggaactggc ccagtgtcat cgggcgtcac gaggtaacag tcgaactcgc cgccgtcgcg
     1741 cgagcggatc ctgatgtcct tgcgctgcat ggtgtcctcc tttggatttt ccgttccaac
     1801 gagtcgtgca ccgtatcacc gggcgcgtcg gcttgtccac cgcatgcgca attcgacttc
     1861 ccgcgccgct tcggtgagaa tccgcaaaac atttcgccgg ggcagtcgat ggatttttcg
     1921 ctcaacgaag cgcagcaaac gtggcagcgg agggcgcgcg aatttgcacg cgaggagatc
     1981 cggtcgatct cgctagagcg cgaccagatg gaaggtggcc ttgcaccgtg ggactgggga
     2041 atcatgaaga aaggctcggc gctcgggttc cgcacgctcg cggtcccgcg cgactggggc
     2101 gggccgggcg cggacttcgt cacgcaaggc atcgtgatgg ccgagctcgc acgcggggac
     2161 agcgcgatct ccaaggcctt cagccagaac tggaaatgga gccatctcat cgcggagaac
     2221 tgcacgaacg accagaagga gcgcttcctc aagccctttc tcgccgacga caccttcgtc
     2281 atgggcgcgg cgagcaccga gcccaacgca ggctccgaca accgttaccc gccggaagac
     2341 gaccccaaag cgggctggcg actgcgcgcg gagcggcgcg gggacgagtg gctgctgaac
     2401 ggggagaaga ccttcatcgc gaatggcagc atcggcaagc ttttcttcgt gaccgcgcgc
     2461 accaatcccg atgtgccgat gcgtgacggc acgacggtct tcatggtgcc gctcggcaca
     2521 ccgggcttcc gggtggggaa gatcttcaac aagagcggct ggcgtttcta tcagaatgca
     2581 gagctcgtct tcgaaaacgc gcgtgtgccg catgcgaaca tcgtcggcga ggtgaatggc
     2641 ggcgtgaaag tgaggagtgg agcttcagcc gagttcaacg atttcgagct cgctgccaac
     2701 gcacttggcg tgtgcgacgc ggcgtgcgag caggccatgg cgcttgcccg gaaagcgcgc
     2761 cgcggcggaa agcggcttta cgaccaccag ttgatccagc tcaggatcaa cgagatgcac
     2821 ctgctgaccg aggcgctccg ctcctatgtg atgcgcaccg cctgggagat ggaccagcgc
     2881 cggcagggcg cgaatgcggt gctcgtgatg aactattcga ccgacgtgat ccagcgcgtc
     2941 acgcgactca atctggaaat ccatcgcgcg acggacggcg tgatggacgc catggcacac
     3001 aagctcgtgc gcgacgccat catctggtcg catcttgccg gggatacctc ccagcgcctc
     3061 aaggccatgc gacgcctgct cgcgcactga ttcatttccg taaggtcgtc gcggttccga
     3121 cgcgcggcgt caattcctcg tcttcgctgc cgtccgtgga gccatagcgc agacccggcc
     3181 cgtccggtcg cctcttggcc attcaagagc ttacggcacg tgtg
//
LOCUS       DASURG010000245         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1166109, whole genome shotgun sequence.
ACCESSION   DASURG010000245 DASURG010000000
VERSION     DASURG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166109"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFY80_04185"
     CDS             complement(<1..656)
                     /locus_tag="VFY80_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6007135.1"
                     /translation="MRIAMIGAGYVGLVSGACFSEFGHHVTCVDQDELKIGRLRRNEI
                     PIYEPGLDVLVARNAAAGRLKFERDLSAAVGDSDLVFIAVGTPSRRGDGHADLTYVRE
                     AAKQIAHAMRGYTVVVTKSTVPVGTGREVAEIIRANNPKADFDVVSNPEFLREGSAID
                     DFMRPNRVVIGAESERARGKMRELYRPLYLIETPILFTGLETAELVKYAANGFLAMKI
                     S"
     gene            complement(678..1562)
                     /gene="galU"
                     /locus_tag="VFY80_04190"
     CDS             complement(678..1562)
                     /gene="galU"
                     /locus_tag="VFY80_04190"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX6007136.1"
                     /translation="MRKPLRKAIFPVGGLGTRFLPATKAMPKEMLPVVDKPLIQYAVE
                     EARAAGIEEMIFVTGRGKSAIEDHFDYSYELQETLKERSKDAELALIDNLLPNPGEFA
                     YVRQQEARGLGHAVWCARNLVGNEPFAVLLADDLVQAGTPCLKQMVEVYNEVGGNVVA
                     VMDVPQAHTDRYGILKTGEARGRLVEAVGLIEKPKPEVAPSTLSIIGRYILQPDVFAH
                     LDQQTPGAGGEIQLTDAMAKMCGRGRFHGYRFEGRRFDCGDKAGFIEANIAFALNHPK
                     IGSKVRESVLEIAQGLKR"
     gene            complement(1888..>2094)
                     /locus_tag="VFY80_04195"
     CDS             complement(1888..>2094)
                     /locus_tag="VFY80_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6007137.1"
                     /translation="NWESTRFNLPALIRERIPAIRKHETAVILHNDAVVQGLSEAPFM
                     KDVKHWAVLTIGTGLGNASFENKN"
BASE COUNT          345 a          740 c          622 g          387 t
ORIGIN      
        1 gagatcttca tggcgaggaa gccgttggcg gcgtatttga ccagctccgc cgtctccagc
       61 ccggtgaaca ggatcggcgt ctcaatcagg taaagcggcc gatagagctc gcgcatcttg
      121 ccgcgcgcac gctcgctctc cgcgccgatc acgacccggt tggggcgcat gaaatcgtca
      181 atcgccgaac cctcccgcag gaactccgga ttggagacca cgtcgaaatc cgccttgggg
      241 ttgttcgcgc ggatgatctc ggctacctcc cgcccggttc ccaccggcac cgtggacttt
      301 gtcacgacga ccgtgtagcc gcgcatggcg tgcgcgatct gcttcgcagc ttcgcggaca
      361 taggtcaggt cggcgtgacc gtcgccgcgc cggctcggcg tgccgacggc gatgaagacc
      421 aggtccgaat ccccgaccgc cgccgacaga tccctctcga acttgagccg gccggccgcg
      481 gcgttgcgcg ccaccagcac gtcgaggccg ggctcgtaga tcggaatctc gttgcggcgg
      541 agccgcccga tcttcagctc atcctgatcg acgcaggtca catggtggcc gaactcggag
      601 aagcaggcgc ccgacaccag gccgacatag ccggctccga tcattgcgat tctcatggcg
      661 cctgtcctcc ctgccggtca gcgcttcaag ccttgtgcga tctcgagcac ggactcgcgc
      721 accttcgatc cgatcttcgg gtgattgagg gcgaaggcga tgttggcctc gatgaagccg
      781 gccttgtcgc cgcaatcgaa gcgccggccc tcgaagcggt agccgtggaa ccggccgcgg
      841 ccgcacatct tggccatcgc gtcggtcagc tggatctcgc cgcccgcccc cggcgtctgc
      901 tgatcgagat gcgcgaacac gtccggctgc aggatgtagc gcccgatgat cgacagcgtc
      961 gagggcgcca cctccggctt cggcttctcg atgagcccca ccgcctcgac cagccgcccg
     1021 cgcgcctcgc cggtcttgag gatgccgtag cggtcggtgt gcgcctgcgg cacatccatc
     1081 accgccacca cgttgccgcc gacctcgttg tagacctcca ccatctgctt caggcacggt
     1141 gttcccgcct gcaccaggtc gtccgccagc agcaccgcga acggctcgtt gcccaccagg
     1201 ttgcgcgcgc accacaccgc atggcccagc ccgcgcgcct cctgctggcg cacataggcg
     1261 aactcgcccg ggttcggcag cagattgtcg atcagcgcca gctcggcatc cttgctgcgc
     1321 tccttcaagg tctcctgcag ctcgtaggaa tagtcgaagt gatcctcgat cgccgacttg
     1381 ccgcgcccgg tcacgaagat catctcctcg atccccgccg cccgcgcctc ctccaccgca
     1441 tactggatca gcggcttgtc caccaccggc agcatctcct tcggcatcgc cttggtcgcc
     1501 ggcagaaacc gcgtccccaa cccgccaacc ggaaagatcg ccttacgcag cggcttacgc
     1561 atagatcacc tctttgatgt tcccgtctgt cgcagcccaa atcgctgcgg cggcgtcttc
     1621 gcgcctgcga ccgaatatgc ataaagggta ccgcatgcgc gcttcaaccc gacagcgaag
     1681 gtttgatgac gatgtttgcc ctgtgtcaca ggcgcaaact gcgatacgcg cagaaagcgt
     1741 gcggcacgcg ttataggttt gcgcggtctg cttaccgagc accaccggga cacaatgtgc
     1801 gccaattagg cagcgcctct cgcataccca cgcggactct gtgacatttc agaggcacga
     1861 cgtcggtgcc gctcgcgcgc ggccccatca gttcttgttc tcgaagctcg cattgccgag
     1921 gccggtgccg atcgtgagca ctgcccaatg cttcacgtct ttcataaagg gcgcttcgct
     1981 gaggccctgc acgaccgcat cgttgtgcag gatcacggct gtctcgtgct tgcggatcgc
     2041 ggggatgcgt tcgcggatca gcgcgggcag gttgaagcgc gtgctttccc aatt
//
LOCUS       DASURG010000246         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1370568, whole genome shotgun sequence.
ACCESSION   DASURG010000246 DASURG010000000
VERSION     DASURG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370568"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFY80_04200"
     CDS             <1..437
                     /locus_tag="VFY80_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX6007138.1"
                     /translation="ALLWHEVLAAWKVSEAKNVPTIMALYQAMDQVLQAQSEQLAIPR
                     RFSGDMREIWALQPRFLHRSGQRPYRLLQQQRFRAGYDFLLLRCESGEVEPEIGEWWT
                     RFQRAGEEERAAMLVREAAPGKRRRKRRRRKQDHGASAKVEE"
     gene            493..1005
                     /gene="folK"
                     /locus_tag="VFY80_04205"
     CDS             493..1005
                     /gene="folK"
                     /locus_tag="VFY80_04205"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX6007139.1"
                     /translation="MSAPHAAVAAYVALGSNLGHPADHIERALYELDALPGTRLERTS
                     SVYRSPPVGYREQPDFLNAVARVETQLEPCPLLEELLAIERRHGRMREFPNAPRTLDL
                     DLLLYGTRIVREAGLTVPHARMLERAFVIVPLVEIAPDVEIPGFGRARDALSKVDAAS
                     VARIEAGSRR"
     gene            1002..1646
                     /locus_tag="VFY80_04210"
     CDS             1002..1646
                     /locus_tag="VFY80_04210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX6007140.1"
                     /translation="MSLPERFRYIVVEGPIGAGKTSLARLLSERCGARLMLEEPEANP
                     FLPGFYQDAERYALPTQLYFLFQRVNQVRALRQTHLFERVTVADFMLDKDPIFARLTL
                     GDEEWKLYDQIYTHLKPQAPTPDLTIYLQATTDTLVERVRRRATPYERTMTEAYLARL
                     AETYMRYFYQYTAAPLLIVNSENLNFVDSPRDFDLLLERISAMRGPREFFSLGN"
     gene            1659..2456
                     /gene="panB"
                     /locus_tag="VFY80_04215"
     CDS             1659..2456
                     /gene="panB"
                     /locus_tag="VFY80_04215"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6007141.1"
                     /translation="MRITLNTLQKMAQEGNKITMLTCYDASFAALLEEAGVESLLIGD
                     SLGMVLQGHESTLPVTLRDIVYHTACVARGAKRAFIIGDMPFGTFQISAQEAFRNAAD
                     IMAAGAHMVKIEGGKPMLETIEFMTHRGIPVCGHLGLTPQSVHQLGGYRVQGKGDAAA
                     QLMIEEATLIEQAGAGMIVLEAIPAPLAGEITKSVRVPTIGIGAGVECSGQVLVLHDM
                     LDIYPGKKAKFVKNYMRLAGTIQGAAETYVREVKAKTYPGPEHSFAS"
     gene            2464..>2587
                     /locus_tag="VFY80_04220"
     CDS             2464..>2587
                     /locus_tag="VFY80_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6007142.1"
                     /translation="MDIVHSVADLRARLQREPNNVFVPTMGNLHEGHIRLIEVAE"
BASE COUNT          480 a          825 c          835 g          447 t
ORIGIN      
        1 ccgcacttct gtggcacgag gtgctggccg cctggaaggt aagcgaagcc aagaacgtgc
       61 caaccatcat ggcgctctac caggcgatgg accaggtgct gcaggcacag tccgaacagc
      121 tcgccattcc gcggcgcttc agcggcgaca tgcgggagat ctgggcgctc cagccacgct
      181 ttctgcaccg ctcggggcag cgcccctatc ggctgctcca gcaacagcgc ttccgggcgg
      241 gctatgactt cctgctgctc cgctgcgaga gcggtgaagt tgaacccgag atcggcgagt
      301 ggtggacgcg ttttcagcgc gccggggaag aagaacgtgc agcgatgctc gtgcgcgagg
      361 cggccccggg caagcggcgc cgcaagcggc ggcgcagaaa gcaagaccac ggcgcgagcg
      421 cgaaggtgga agaatgagag ccggtcgggg ccgctccaat cgatgccgcg catcgctgac
      481 ggagcgtaaa atttgagcgc gcctcacgcg gcggtcgctg catacgttgc gcttggcagc
      541 aatctcggac atccggccga tcacatcgaa cgcgccctgt acgagctcga cgcccttccc
      601 gggacgcggc ttgaacgcac ttcgtcggtc taccgcagcc caccggtggg ttatcgcgag
      661 caacccgatt ttctgaatgc cgtggctcga gtggagaccc agctcgaacc gtgcccgctg
      721 ctcgaagaac tgctcgcgat cgagcgccgt cacgggcgca tgcgtgagtt tccgaatgcg
      781 ccgcgcacac tcgatctcga ccttctgcta tacgggacgc gcatcgtgcg cgaggcgggc
      841 ctcaccgtgc cgcatgcgcg gatgctggag cgcgccttcg tgatcgtgcc gctcgtggag
      901 attgcgcccg acgtcgaaat tccgggcttc gggcgtgcgc gtgatgcgtt gtcgaaggtg
      961 gacgcggcga gcgtcgcgcg aatcgaagcg ggctcacggc gatgagcctc cccgaaagat
     1021 ttcgctacat cgtcgtcgag gggccgattg gcgcaggcaa gaccagtctc gcgcggctct
     1081 tgagcgagcg ctgcggcgcg cggctcatgc tcgaggagcc cgaggccaat cctttcctcc
     1141 ctggcttcta ccaggacgcc gagcgttatg ctctgccgac gcagctctat tttctgttcc
     1201 agcgcgtgaa ccaggtgcgc gcgctgcgcc agacgcatct cttcgagcgc gttaccgtag
     1261 ctgatttcat gctcgacaag gatcccattt tcgcgcgcct cacgctggga gacgaagagt
     1321 ggaagctcta cgatcagatc tacacgcacc tgaagccgca ggcgccgacg ccggatctca
     1381 ccatctacct ccaggcaact accgacacgc tggtcgagcg ggtgaggcgc cgcgcgacac
     1441 cctacgaaag gaccatgact gaggcatatc tggcgcggct tgccgagacc tacatgcgct
     1501 acttctatca gtacaccgcg gcgccgctgc tcatcgtcaa ttccgagaac ctcaactttg
     1561 ttgattcgcc ccgagatttt gatttattgt tggagcgcat cagtgccatg cgcgggccgc
     1621 gcgagttctt ctccctcgga aactaaaggc gattactcat gcgtatcacc ctgaacacgc
     1681 tgcagaagat ggcgcaggag ggcaacaaga tcacgatgct gacctgctat gacgcgagct
     1741 tcgcggcgct gctggaggag gcgggggtgg agtcgctcct catcggcgac tcgctgggca
     1801 tggtgctgca gggacacgaa tcaacgctgc ccgtgacgct gcgcgacatc gtttatcaca
     1861 ccgcgtgcgt tgcgagaggc gcgaagcgtg cgttcatcat cggcgacatg ccgttcggca
     1921 ctttccagat ttcggcgcag gaagccttcc gcaacgccgc cgacatcatg gccgccggcg
     1981 cgcacatggt gaagatcgag ggcggcaagc ccatgctcga gaccatcgag ttcatgacgc
     2041 atcggggtat cccggtatgc ggccatctcg gcttgacccc gcagtcggtg catcagctcg
     2101 gtggctaccg cgtgcagggc aagggcgacg cggcggccca gctcatgatc gaggaagcga
     2161 cgctgatcga gcaggcgggc gccggcatga tcgtgctcga ggcaataccc gcgccgcttg
     2221 ccggcgagat cacgaagtcg gtgcgcgtgc caacgatcgg catcggcgcc ggcgtcgaat
     2281 gctcgggtca ggtgctggtg ctgcacgaca tgctcgacat ctatcccggc aagaaagcga
     2341 agttcgtcaa gaactacatg cggctcgccg gcaccatcca gggcgcggcg gaaacgtacg
     2401 tgcgcgaggt aaaggctaag acctaccccg gccccgagca ctccttcgcg agctgagcgc
     2461 ttcatggaca tcgttcactc cgtcgccgat ctgcgggcgc ggctccagcg cgagccgaac
     2521 aacgtcttcg tgccgacgat gggcaacctg cacgaagggc acatcaggct gatcgaagtc
     2581 gccgagc
//
LOCUS       DASURG010000247         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_962773, whole genome shotgun sequence.
ACCESSION   DASURG010000247 DASURG010000000
VERSION     DASURG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962773"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFY80_04225"
     CDS             <1..771
                     /locus_tag="VFY80_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX6007143.1"
                     /translation="ARILKRARIDEALWRSIIGRYPFLAVLSHEEAARLRECVALFLH
                     DKAIHGAGGLAMRDEIRLAIAAQACILILNLDLDYYRGWVEIIVYPDEFVASYEYVDE
                     DGVVHAVREPMSGESWLRGPVILSWADAVDAGYAGYNVVIHEFAHKLDMLNGDANGFP
                     PLHADMSRHEWSRAFSEGYADFCARVDRGDPLPVDDYAVEDPAEFFAVLSEAFFETPE
                     TVQRMYPAIYRELARFYRQDPAARRVCCAPAVSAAVTP"
     gene            768..2753
                     /locus_tag="VFY80_04230"
     CDS             768..2753
                     /locus_tag="VFY80_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6007144.1"
                     /translation="MSAPGTTSSRSSRILMVGFGILLAMIVGITVATITVTRMDETLQ
                     SGGLARSALLASVALGIVALLIGAYIAARVVREVIRTERALALEKELAQVTLHSIGDG
                     VITTDAEGRVEYLNPVAERYTEWGTTDARGRPLAEVFRVIDERTGEPVDPVRSAMGGI
                     AGDNDEPASMRLVSRNDRAWPIRFSCAPINAGGQRALGTIVVFHDISQIREMAQQLLW
                     QASHDALTGLVNRREFERRLAALIETARGQGREHALMFMDLDNFKAVNDTCGHGAGDE
                     LLRQLTAVMLTRMRGSDTLARLGGDEFGALLESCPPDQGLRIAHAMRETVRDFRFVWE
                     NKTFSVGVSIGLVPIDAASGSVREVLALADASCYEAKSKGRDRVQVYRPQETDAAGKH
                     SELQLVSQINRAFELGEFRLFRQRILPLAPAAAHQPYYEVLVRMVDRSGALVPPTGFM
                     PAAERYNLLTSIERWVMSALVEYLHRQWQSGAIPHQPGPDGTRGYYAVNLSGASINDK
                     SFPDFLKRLLTRYALPHGLLCFEITETTAISNLNNAAELMHELKGMGYRFALDDFGTG
                     MSSFAYLKYLPVDYLKIAGMFVKDMAEDAMDCAIVESINRISHILGMQTVAESVESAQ
                     TLERVRALKVDFAQGYYIAEPEAMVTGEATHPERLTA"
     gene            2750..3802
                     /locus_tag="VFY80_04235"
     CDS             2750..3802
                     /locus_tag="VFY80_04235"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF001415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX6007145.1"
                     /translation="MKRRTEPYGLAADVGGTKTALVLARPQRAWPAIVAEQVYPSQDY
                     ETFESVIAEFLARPETRDCDGRIAAACACIAGPVEGDRATTTNLRWTIEARALSARFG
                     IPAVRLVNDFAAVAFGIGRIDARDLEPLQPGEANEHGARAVMGAGTGLGVALMSWNGR
                     EYAVHPSEGGHADFAPVDALQDGLLEYLRGEYGRVSYERVLSGTGLAQIFDYLATREE
                     PSAAMRDAVRSASDAAAAVSEFGLEGRDPVAARALDVFAAVYGAFAGNVALTVLARGG
                     VYIAGGIAPKIAGKLKDGTFVRAFTSKGRFSALLETMPVSIVMNAKVGLYGALDLARR
                     LVQPAAISQRQRVRDR"
     gene            complement(3799..4410)
                     /gene="mobA"
                     /locus_tag="VFY80_04240"
     CDS             complement(3799..4410)
                     /gene="mobA"
                     /locus_tag="VFY80_04240"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX6007146.1"
                     /translation="MTSTLSSRALPVTGVVLAGGLGRRMGGVDKGLQLLRGKPLVAWV
                     IERFAPQVDEVLVNANQNLDRYQALGHRVIRDEIDGFAGPLAGLHCALGAARYERIAT
                     VPCDSPFLPADLVSRLSAALDAEHADLAVARTGNQPHPVFCLCRKSVLPDLGAFLAAG
                     GRKIDAWYARLRTVEVPFDEEAQAFSNINTREELSSFEGEREA"
     gene            complement(4446..>4946)
                     /locus_tag="VFY80_04245"
     CDS             complement(4446..>4946)
                     /locus_tag="VFY80_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX6007147.1"
                     /translation="TGCGQGTVFGDLMEEIDSIRLRDDITLDEDTLYALLDAVRRHET
                     VYKSAGAVHGCALADGPEILYFIEDVGRHNAVDAIAGQMWLDGVEGSDKVFYTTGRLT
                     SEMVIKAAQMRIPFLVSRSGLTQMGFEVANKVGITMFGRAVNRHYLLFTGRHRFVKTV
                     TPTMLA"
BASE COUNT          843 a         1573 c         1677 g          853 t
ORIGIN      
        1 gcgcgcattc tgaagcgcgc tcggatcgac gaagcgctgt ggcggtcgat catcggccgc
       61 tatcccttcc tcgccgtgct gtctcatgaa gaggcagccc ggctgcgcga gtgtgtcgcg
      121 ctctttctcc atgacaaggc catccacggc gccgggggtc tcgcgatgcg cgacgagatt
      181 cgactggcca tcgccgcgca ggcatgcatc ctcattctca atctcgacct cgactattac
      241 cgcggctggg tggaaatcat cgtctacccc gacgaattcg tggcgagcta cgaatacgtg
      301 gacgaggacg gggtcgtgca tgccgtgcgc gaaccgatgt ccggtgaatc gtggctgcgt
      361 ggaccggtga ttctctcgtg ggcggatgcg gtggatgcgg gatatgccgg ctacaatgtg
      421 gtcatacacg aatttgcgca caagcttgac atgctgaacg gcgatgccaa cgggtttcct
      481 ccgctccatg cggacatgag ccgtcacgag tggtcgcgtg ccttctccga gggatacgcg
      541 gatttttgcg cgcgcgtgga tcgcggcgat ccactgcccg tcgatgatta cgcggtggag
      601 gatccggcgg agttctttgc cgtattgagc gaggcgtttt tcgaaacgcc cgagaccgtc
      661 cagcggatgt atcccgcgat ctaccgcgag ctggcgcgct tttaccgcca ggaccctgcg
      721 gcccggcgcg tgtgctgtgc gccggccgtc tccgcggctg tcacgccatg agcgctcccg
      781 gcacgacctc gtcgcgttcg agccgcatcc tgatggttgg gttcggtatc ctgctcgcga
      841 tgatcgtggg catcacggtc gctaccatca cggtcacacg catggatgag acgctgcaga
      901 gcggcgggct tgcgcggagc gcgctgctcg cgagcgtcgc cctcgggatc gtcgcacttc
      961 tcatcggcgc atacatcgcg gcccgggtcg tgcgggaagt gatccgcacc gagcgcgcgc
     1021 ttgcgctgga aaaggagctc gcccaggtca cgctccattc catcggagac ggcgtcatca
     1081 cgaccgacgc cgaaggccgc gtggagtatc tcaatccggt ggcggagcgc tataccgagt
     1141 gggggacgac cgatgcgcgg ggccggccgc tggcggaagt ctttcgggtc atcgatgagc
     1201 gcactggaga gccggtcgat cccgtccgtt cggcgatggg cggcatcgca ggcgataacg
     1261 acgagcctgc atcgatgcgg ctcgtgagtc gcaacgatcg cgcgtggccc atccgctttt
     1321 cctgcgcgcc catcaatgcg gggggacaac gagcgctcgg cacgatcgtc gtctttcacg
     1381 acatcagcca gatccgggag atggcgcagc agctgctgtg gcaggcgagt cacgatgcgc
     1441 tgaccgggct cgtcaaccgg cgcgagttcg agcggaggct cgccgcgctc atcgagaccg
     1501 cacgcggcca gggccgcgag cacgccctca tgttcatgga tctcgacaat ttcaaggcag
     1561 tcaacgatac ctgcggccac ggggcgggag acgagctgct gcgccagttg accgccgtca
     1621 tgctgacccg catgcggggc agcgacacgc tggcgcggct ggggggagac gagttcggcg
     1681 cgctgctcga atcctgcccg ccggaccagg ggctgcgtat cgcgcacgcc atgcgtgaga
     1741 ccgtgcgcga cttccgtttc gtgtgggaga acaagacctt cagcgtcggc gtcagcatcg
     1801 gacttgtccc catcgatgcg gcgagcggca gcgtgcgcga agtgctcgcg ctggcggatg
     1861 cctcctgtta tgaggcgaag agcaaggggc gcgaccgcgt gcaggtctac cgcccgcagg
     1921 agaccgatgc ggcgggcaag cacagcgagc tgcagctcgt gtcgcagatc aatcgcgcct
     1981 tcgagctcgg cgaattcagg ctcttccgac agcgtattct gccgctcgca ccggccgctg
     2041 cccaccagcc gtactacgaa gtgctggtgc gcatggtgga tcggtcgggc gcgctcgtgc
     2101 cgccgaccgg cttcatgccc gccgccgagc gctacaacct gctcacctcg atcgagcgct
     2161 gggtgatgag cgcgctcgtg gagtacctgc atcgccagtg gcagtcgggc gcgattccgc
     2221 accagcccgg tccggacggc acgcgcggct actacgcggt gaatctttcc ggcgcgagca
     2281 tcaacgacaa aagcttcccc gatttcctga agcggctgct cacacgctat gcgctgccgc
     2341 acggactgct ctgtttcgag atcacggaga ctaccgcgat atccaatctc aataacgcgg
     2401 ctgagctgat gcacgagctg aaagggatgg gctatcgctt tgcgctcgac gatttcggaa
     2461 ccggcatgtc ctccttcgcc tatctcaagt atctcccggt cgattacctg aagatcgccg
     2521 gcatgttcgt gaaggacatg gcggaggacg ccatggactg cgcgatcgtg gagtccatca
     2581 accgtatcag ccacatcctg ggcatgcaga ccgttgccga atccgtcgaa agcgcgcaaa
     2641 cgctggagcg ggtgagggcg ctcaaggtcg atttcgcaca aggctattac atcgccgagc
     2701 ccgaggcgat ggtgacgggc gaagcgactc atcccgagcg cctcacggca tgaagcgccg
     2761 cacggagccc tatgggctcg ccgcggatgt ggggggcacc aagactgcgc ttgtgctcgc
     2821 gcgcccccag cgcgcgtggc cggccatcgt ggcggagcag gtgtacccaa gccaggacta
     2881 cgaaaccttc gaaagcgtca tcgcggagtt tctggcccgg cccgaaaccc gggactgcga
     2941 cggcaggatc gcggccgcct gtgcgtgcat cgcggggccg gtggagggcg atcgtgctac
     3001 gacgacgaac ctgcgctgga ccatcgaagc acgcgcgctg agcgcgcgct tcggtatccc
     3061 ggcggtgagg ctcgtgaatg actttgctgc agtcgccttc ggcatcgggc ggatcgatgc
     3121 gcgggatttg gagccgctgc agccgggcga ggcgaacgag cacggcgcgc gcgcggtgat
     3181 gggcgcgggc acgggcctcg gcgtcgcgct catgagctgg aacggccgcg aatacgcagt
     3241 gcacccctcc gaaggggggc acgcggattt tgcgccggtc gatgcgctgc aggatgggct
     3301 gctggagtat ctgcgcggcg agtacggccg cgtctcctac gagcgggtgc tttcgggaac
     3361 ggggctcgcg cagattttcg attaccttgc gacgcgcgaa gagccgtcag ccgccatgcg
     3421 ggacgcggtg cgcagcgcct cagacgcggc cgccgccgtg agcgagttcg gactggaggg
     3481 gcgcgatccg gttgcggcgc gcgcgctcga tgtattcgcc gccgtgtatg gcgcctttgc
     3541 gggaaacgtt gcgctcacgg tgcttgcgcg gggcggcgtc tatatcgcgg gcggcatcgc
     3601 gcccaagatt gcgggaaagc tcaaggacgg cacgttcgtg cgcgcattca ccagcaaagg
     3661 ccggttcagc gctcttctgg agaccatgcc cgtcagcatc gtcatgaacg ccaaggtcgg
     3721 cctctacggc gcactcgatc tggcacggcg gctcgtgcag ccggcggcaa tctcacagcg
     3781 gcagcgcgtt cgagaccgtt aagcttcgcg ttcgccttcg aagctcgaga gttcttcgcg
     3841 cgtattgatg ttactgaacg cctgagcctc ctcgtcgaac ggcacttcga cggttcggag
     3901 gcgcgcgtac caggcgtcga tcttgcggcc gccggcggcg agaaaagcgc cgagatccgg
     3961 cagcacgctc ttgcggcaca ggcaaaacac gggatggggc tggttgccgg tacgggcgac
     4021 ggcaaggtcg gcgtgctctg cgtcgagcgc ggcggaaagc cgggaaacga gatctgccgg
     4081 gaggaacggc gaatcgcacg gcacggtcgc gatccgctcg taacgcgccg cgccaagcgc
     4141 gcagtgcagg ccggccagag gaccggcgaa accatcgatt tcgtcgcgga tgacgcgatg
     4201 gccgagcgcc tgatagcgat cgagattctg gttcgcattg accagcactt catcgacctg
     4261 cggcgcgaag cgctcgatca cccacgcgac gagcggcttg ccgcgcagca attgcagccc
     4321 cttatcgaca ccgcccatgc gccgaccgag accgcccgcg agcacgaccc cggtgaccgg
     4381 gagtgcgcgg ctggagagcg tgctcgtcat cgggtaatca aatcgagccg ctcgcgcacg
     4441 ggctcttacg cgagcatcgt cggcgtgacg gtcttgacga aacgatgccg gccggtgaag
     4501 agcaggtaat gccggttgac cgcgcgcccg aacatcgtga taccgacctt gttcgcgacc
     4561 tcgaacccca tctgggtcaa cccggaacgc gagacgagga agggaatgcg catctgcgcc
     4621 gccttgatca ccatctcgga tgtgagccgt ccggtggtat agaagacctt gtccgagccc
     4681 tcgacgccgt ccagccacat ctgcccggcg atggcatcga cggcattgtg ccgcccgacg
     4741 tcctcgatga agtagaggat ttcggggcca tcggcaagcg cgcagccgtg caccgcgccc
     4801 gcgctcttgt agaccgtttc gtgacgccgc actgcatcga ggagcgcata cagcgtgtcc
     4861 tcgtccagcg tgatgtcgtc gcgcaggcgg atcgagtcga tctcctccat gagatcgccg
     4921 aatacggttc cctgaccgca gcccgt
//
LOCUS       DASURG010000248         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_757521, whole genome shotgun sequence.
ACCESSION   DASURG010000248 DASURG010000000
VERSION     DASURG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757521"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFY80_04250"
     CDS             complement(<1..805)
                     /locus_tag="VFY80_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX6007148.1"
                     /translation="MQEVLIESLDQEGRGVAHRDGKTLFIEGALPAERVEYAPYRKKP
                     SFELATLSHVLKSSPFRATPRCAFFGTCGGCSLQHLDTRAQVAAKQRVLEDNLWHIGR
                     VVPQLVLPAIHGPAWGYRHRARLSVRHVPKKGGILVGFHEKRSSYVADMKSCEILPPH
                     VSALLVPLRETLGRLSIRTRLPQIEVSVGEESTVLVLRILEPLSAEDEKLLRVFAGQH
                     AVEIYLQTAGPDSARPFEPQDARALYYALPEFDVRLQFAPTDFTQVNHAV"
     gene            complement(876..1667)
                     /locus_tag="VFY80_04255"
     CDS             complement(876..1667)
                     /locus_tag="VFY80_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX6007149.1"
                     /translation="MIPLLVFDIETVPDVAGLRKLHGLDDSVSDASVADMAFQLRRQS
                     TGSDFLPLHLQRVIAISCALRERDSFRIWSLGGPQQSEAELIQRFFDGLEKYTPQLVS
                     WNGCGFDLPVLHYRGLIHGVKAPRYWDMGDGDHRDSRDFKWNNYISRYHTRHLDLMDL
                     LALYQPRANAPLDSIAQLLGLPGKLGMEGAEVWDAFRAGRLEEIRCYCETDVANTYLV
                     FLRFQLMRGALDDQHYAAECALVRSVLERSPESHWRTFLDRWSRG"
     gene            complement(1687..2574)
                     /gene="cysM"
                     /locus_tag="VFY80_04260"
     CDS             complement(1687..2574)
                     /gene="cysM"
                     /locus_tag="VFY80_04260"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX6007150.1"
                     /translation="MLKTLEQFVGDTPLVKLKRIGADTGNALLAKLEGNNPAGSVKDR
                     PALSMIRRAQERGDIKPGDTLIEPTSGNTGIALAMCAAMMGYRMILVMPEHLSAERRQ
                     TMAAFGAEFILTPREGGMEAARDIAEKMRDEGKGVILDQFANPDNPLAHYEGTGPEIW
                     RDTDGKVTHFVSSMGTTGTIMGVSRYLKERDPAIRIVGCQPTDGAQIPGIRKWPPAYL
                     PKICDWSRVDEVIEVSQRDAEEMTRRLARDEGIFAGISSGGAVWAALQVAEKVRDAVI
                     VTIVCDRGDRYLTTGVFPA"
     gene            complement(2592..>3170)
                     /locus_tag="VFY80_04265"
     CDS             complement(2592..>3170)
                     /locus_tag="VFY80_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6007151.1"
                     /translation="GYSKFLFDQLVRRRVGAFTAQVVGLRYFNVYGGREQHKGRMASV
                     AFHFFNQYRAKGTVSLFEGSGGYGDGEQRRDFVSVEDAVAINLYFLDHPERSGIYNAG
                     TGRAQSFNDVAAAAVNACRRARGEPALTLADMQRQGIVRYIPFPADLRNKYQSYTQAD
                     LTALRDAGYTAPFLTVEQGVARYCERLLAHPA"
BASE COUNT          554 a         1027 c         1016 g          573 t
ORIGIN      
        1 tgacagcgtg gttcacctgc gtgaaatcgg tcggcgcgaa ctgcagtctc acatcgaatt
       61 cgggcagagc gtaatagagc gctcttgcgt cctgcggttc gaatggccgg gcggaatcgg
      121 ggccggccgt ctgcaggtag atttcgaccg catgctgccc cgcaaacacc ctcagcagct
      181 tctcgtcttc tgcggaaagc ggctcgagta tccgcaagac aagcacagtg ctttcctcgc
      241 cgacggagac ctcgatctgc gggagccgag tgcggatgga gaggcggccc agcgtctcgc
      301 gcagcggcac gagcaacgcc gacacgtgcg gaggcaggat ttcgcagctc ttcatgtcgg
      361 cgacgtagct gctgcgcttc tcatgaaagc ccaccagaat gccgcctttc ttgggcacgt
      421 gacgtaccga aagccgcgcg cgatgccggt atccccatgc cggcccgtgg atcgccggga
      481 gtacgagctg gggaacgact ctgccgatgt gccagaggtt gtcttccagc acccgctgct
      541 tcgccgcgac ctgtgcgcgg gtatccaggt gctggaggct gcaaccgccg caggtgccga
      601 agaacgcaca gcgcggcgtt gcgcggaacg ggctcgattt gagcacgtga ctcaacgtcg
      661 cgagttcgaa gctcggcttc ttccggtacg gcgcgtattc gacgcgctcg gcgggcagcg
      721 cgccttcgat gaaaagggtt ttgccgtcgc ggtgcgcaac accgcgtccc tcctggtcga
      781 gggattcgat cagtacttcc tgcatgggtg tttcccgcgg cggcggagtc aaaatgggcc
      841 gcgaaacgcg agaagcgaag tttagcgcaa cacgctcagc cgcgcgacca acgatcgagg
      901 aacgttctcc agtgcgattc gggcgagcgc tcgagcacgc tccgcaccag cgcgcactcc
      961 gcggcatagt gctgatcgtc cagcgcgccg cgcatcaact gaaaccgcag aaagacgaga
     1021 tacgtgttcg ccacgtcggt ttcgcagtag cagcggatct cctcgaggcg gccggcgcgg
     1081 aaggcgtccc atacttcagc cccttccatc ccgagcttgc ccggaaggcc aaggagctgc
     1141 gcgatcgaat cgagtggggc attcgcccgc ggctggtaga gcgcgagcag atccatgaga
     1201 tcgaggtgac gcgtgtggta gcggctgatg tagttgttcc atttgaagtc gcggctgtcg
     1261 cgatggtcgc cgtcgcccat gtcccagtag cgcggcgcct tcaccccatg gatgaggccg
     1321 cggtagtgaa gcaccggaag gtcgaagccg catccgttcc acgagacgag ctgcggggtg
     1381 tacttctcga gaccgtcgaa gaaccgctgg atgagttccg cttcgctctg ctgcggcccg
     1441 ccgagcgacc agatgcggaa gctgtcgcgc tcgcgcaggg cgcaggaaat ggcgatgacc
     1501 cgctgcagat gaagcgggag gaaatcgctg cccgtcgact ggcgcctcaa ctgaaacgcc
     1561 atgtcggcca cgctcgcatc cgacacgctg tcatcgagcc cgtgcagctt gcgcaggccc
     1621 gcgacgtcgg gcaccgtctc gatatcgaat acgaggagcg gaatcacgtg gtcgaacctg
     1681 tcgtcatcat gccggaaaga cgccggtggt cagatagcga tctccgcgat cgcagacgat
     1741 ggtcacgatg acggcatcgc gcaccttctc cgccacctga agcgccgccc acaccgcgcc
     1801 gccggacgaa atgccggcga atattccctc atcccgtgcg agccgccgcg tcatctcttc
     1861 cgcgtcgcgc tggctcactt cgatcacctc atcgacgcgg ctccagtcgc agatcttcgg
     1921 cagataggct gggggccact tgcgtatccc cgggatctgc gcgccatccg taggctggca
     1981 ccccacgatc cgaatcgcgg ggtcgcgctc cttgaggtag cgcgacacgc ccatgatggt
     2041 gccggtcgtc cccatgctgc ttacgaagtg cgtcaccttg ccgtcggtat cgcgccagat
     2101 ctccggtccc gtaccctcgt agtgtgcgag gggattgtcg ggattcgcga actggtcgag
     2161 gatcacgcct ttcccctcgt cgcgcatctt ctcggcaata tcccgcgccg cttccatccc
     2221 gccctccctg ggggtcagga tgaactccgc gccgaatgcc gccatggtct ggcgccgctc
     2281 ggccgacagg tgttccggca tgacgagtat catgcggtaa cccatcatgg ccgcgcacat
     2341 cgcgagcgcg atgccggtat tgccgctggt gggctcgatc agcgtatcgc ccggtttgat
     2401 gtcaccccgc tcctgcgcgc gtcgaatcat cgagagcgcc ggccggtcct tgaccgagcc
     2461 cgcggggttg ttgccttcga gcttggcaag cagcgcattg cccgtatccg cgccgatccg
     2521 cttgagcttc acgagcggcg tgtcgccaac gaattgttcc agtgtcttca acatgcggga
     2581 tccgtgtatg cctatgccgg atgcgcgagt agccgttcac aatatcgcgc gacgccctgc
     2641 tcgacggtga ggaaaggcgc ggtgtaaccg gcatcgcgca atgccgtcag atcggcctgc
     2701 gtgtagctct ggtacttgtt cctgagatcg gcggggaatg gaatgtagcg cacgatcccc
     2761 tgccgctgca tgtcggccag cgtgagcgcc ggctcgcctc gcgcgcgacg acaggcgttg
     2821 accgcggccg ccgccacgtc gttgaagctc tgcgcgcgtc ccgttccggc attgtaaatg
     2881 cccgagcgct cggggtgatc gaggaaatag aggttgatcg cgaccgcgtc ttcgaccgat
     2941 acgaaatcgc gacgctgctc gccgtcgcca tagccgccgc tgccctcgaa gagcgagacg
     3001 gtgcccttcg cgcgatactg gttgaagaag tgaaaggcga cggacgccat gcggcccttg
     3061 tgctgttcgc gcccaccgta gacgttgaag tagcgcagcc cgacgacctg cgcggtgaac
     3121 gcgcccacgc gccgccgcac gagctggtcg aacaggaact tggaatagcc
//
LOCUS       DASURG010000249         5054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_44006, whole genome shotgun sequence.
ACCESSION   DASURG010000249 DASURG010000000
VERSION     DASURG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44006"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFY80_04270"
     CDS             <1..575
                     /locus_tag="VFY80_04270"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6007152.1"
                     /translation="RGADFCKGRDYQSAPESARDGRPPSARAIIDTMAAPALAAYEAL
                     REAPVPTLAVVQGAASGFGCALACACDIVLAGAGSRFRFPEMNRGLPPTLAMHAVMDR
                     VSARALSYLVYSTAEVDSRTALALGLASMIVPDDELEREAAALVAAISKHPPDAVRTV
                     KSFLRRAPAMDPQARAELAATLFATTLSSR"
     gene            722..4231
                     /gene="dnaE"
                     /locus_tag="VFY80_04275"
     CDS             722..4231
                     /gene="dnaE"
                     /locus_tag="VFY80_04275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX6007153.1"
                     /translation="MQPSFVHLRLHSEYSIADGIVRLDEAVAAAAADGMPALALSDLA
                     NVFGMVKFYKEARAKGVKPIVGCDVWIANQADRDKPSRLLLLCQSRAGYLTLSDLLTR
                     AYRFNQHRGRAEIDSSWFAEVGTEGLIALSGAHLGDVGQALAVGNEEVARRLAHEWSG
                     LFPQRFYIELQRLGAGATTTGGATVPIEACVQRAVRLANALRLPVVATHPVQFIRPDD
                     FQAHEARVCIAEGYTLSDRRRPRRFTPEQYFKSQAEMAALFRDIPAALANSVEIARRC
                     NLTLQLGKSQLPQFPTPNQESLESYLQSRAVAGLESRMARLHSNEAQRAREMPRYRDR
                     LEFELKTITQMGFAGYFLIVADFINWAKGNGVPVGPGRGSGAGSLVAYSLGITDLDPL
                     RYDLLFERFLNPERVSMPDFDIDFCQEGRDRVIEYVRSKYGADSVSQIVTFGTMAAKA
                     VVRDVGRVLDLPYNFCDQIAKLIPFQPGKTVTLRRRTGEPEPNVIYAREVEPQINERE
                     KNEEEVRELLALAEQLEGLTRNVGMHAGGVLIAPGKLTDFCPLYAADGSVSVVSQFDM
                     KDVEAVGLVKFDFLGLTTLTILDWAVKYIEQLDRESKVKLDEIPLDDAETYRVFANAD
                     TTAVFQCESRGMRELMKQAKPDRFEDIVALIALYRPGPMELIPEFIRRKHGGRIEYPD
                     PRVEPILKPTYGIMVYQEQVMQIAQVIGGYSLGSADLLRRAMGKKMPEEMARHRETFV
                     KGAAQSGVPERKANELFDLMEKFAGYGFNRSHAAAYALVAYQTAYLKAHHAAAFSAAN
                     MSAVMADTDKVQLFADDAREHGIVLLPPDINASNYRFEPLPPGENGQCGVVRYGLGAI
                     KGTGEAAIEHIIDARRGGGPFKDLFDFCHRVDKRIVNRRVVESLIRAGALDSIDDHRA
                     RLLASVGTAMESAEQASRSANQVSLFGELAHAGPPAALADVARWSDRERLQNEKLALG
                     FYLSGHPFRSYEEELRQFVTTRLDELAPQPYPVLLAGIIHSLRVQMTRRGRMAVILLD
                     DGRARVELTVFNELFEQHRNALKEDQLLIVEGKVAHDDFSGSLRISAEKLYDLAAARN
                     RYARAIRLTCNGASSGARLRELLAPHRDGSCPVSIVYSNHGAMCEIDLGEKWQVALHD
                     ELIRALGEWLKPENVSIVYATRA"
     gene            4215..4913
                     /locus_tag="VFY80_04280"
     CDS             4215..4913
                     /locus_tag="VFY80_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6007154.1"
                     /translation="MPRARKRSASVRALFAAFLFTAFAPLTHAGEAIWRALNDEIVPL
                     ITRGELHHAERTARQALAEARKTFGDTHRNTETSLASLALALRFRGKHEEAETHYRAA
                     LALREKTIGADHPSTAMLLANLAEVIQSQRRYAEAEKIQRRVLPIFEKAYGEDPKTAT
                     LLNNLGAVLRVQGRHKEAEPLLRRALVMKENTLGPYNPSVANTLTNLAEVLDHLGRRD
                     EAAQMRLRAAAITR"
BASE COUNT          887 a         1702 c         1636 g          829 t
ORIGIN      
        1 ggcgtggcgc cgacttctgc aagggacgcg actaccagtc cgcgccggag agcgcacgcg
       61 acggtcgccc gccctcggcg cgcgcgatca tcgatacaat ggccgcacct gcgctcgctg
      121 cctacgaggc attgcgcgaa gcgccggtac ccaccctcgc cgtggtgcag ggcgcggcct
      181 cgggcttcgg gtgcgcgctc gcctgcgctt gcgatatcgt gctggccggc gcaggcagcc
      241 gctttcgctt tcccgaaatg aatcgcgggc ttccgcccac gctggccatg catgcggtca
      301 tggaccgggt gagcgcgcgc gcgttgagct atctcgtcta ttccaccgcc gaggtcgatt
      361 cccgcacggc gctcgcgctc ggcctggcga gcatgatcgt gcccgatgac gagctcgagc
      421 gcgaggcggc ggcgcttgtc gccgcgattt cgaagcaccc gcccgatgcg gtgcgtacag
      481 tcaaatcgtt cctgaggcgc gcgccggcga tggatcctca ggcacgcgcc gagctggcgg
      541 caacgttgtt cgccaccacg ctgtcctccc gctgatggcg cctgcgccgc cgccatgccg
      601 gcggaatgac gccgcgcatg ccgtacgcga cgccgggtgc tatgtcacgg aatcggtgca
      661 cataaatttt tccgtcacac cggatgaaaa tccgcgccgc gtggttttag aatagcgctt
      721 catgcagccg tcgttcgtgc acctgcgtct ccacagcgag tactccatcg cagacgggat
      781 cgtgcggctc gacgaggcgg tggccgcggc agcggccgac gggatgccgg cgcttgcgct
      841 gagcgacctc gcaaacgtct tcggcatggt gaagttctac aaggaggcgc gggcgaaagg
      901 cgtcaagccc atcgtcggtt gcgacgtctg gatcgcgaac caggccgatc gcgacaagcc
      961 gagtcgcctg ctgctcctct gccagtcccg cgccggatat ctcacgctca gcgatctgct
     1021 gacgcgcgca taccgcttca atcagcatcg cgggcgcgcg gaaatcgaca gcagctggtt
     1081 tgccgaggtc gggaccgaag ggctgatcgc gctttccggg gcacatctgg gcgacgtggg
     1141 tcaggcgctc gcggtcggca atgaggaggt ggcccgcagg ctcgcgcatg agtggagcgg
     1201 gctttttccc cagcgtttct acatcgagct gcagcgtctc ggcgcgggtg cgacgacgac
     1261 cggtggcgcg acagtcccga tcgaagcctg cgtgcagcgg gcggtgcggc tcgccaatgc
     1321 gttgcgcctg ccggtggtcg cgacgcatcc cgtccagttc atccggcccg acgacttcca
     1381 ggcgcacgag gcgcgggtgt gcatcgccga aggctacacg ctttcggacc ggcggcgtcc
     1441 gcggcgcttc acgcccgagc agtacttcaa gtcccaggcg gaaatggcgg cgctcttccg
     1501 ggacattcct gcggcgctcg cgaacagcgt cgaaatcgcg cgccgctgca atctgacgct
     1561 gcaactggga aagagccagc ttccccaatt tcccacgccc aatcaggaaa gcctcgaaag
     1621 ctatctgcaa agccgtgcgg tggcggggct cgagtcgcgt atggcgcggc ttcattcgaa
     1681 cgaggcgcag cgcgcgcgcg agatgccgcg ttaccgcgac cggctcgaat tcgagttgaa
     1741 gaccatcacc cagatgggct ttgccggcta cttcctgatc gtcgccgact tcatcaactg
     1801 ggcgaagggg aatggcgtgc cggtcgggcc ggggcgcgga tccggcgccg gctcgcttgt
     1861 cgcctacagc cttggcatca ccgacctcga tccgctgcgc tacgatctgc tcttcgagcg
     1921 ctttctcaat cccgagcgcg tatcgatgcc ggacttcgac atcgacttct gccaagaagg
     1981 acgggatcgc gtcatcgagt acgtacgcag caagtacggc gccgattcgg tgtcgcagat
     2041 cgtcacgttc ggcacgatgg ccgcgaaagc ggtggtgcgc gatgtcggac gcgtcctcga
     2101 tctcccgtac aacttctgcg accagatcgc gaagctgatt cccttccagc cgggcaagac
     2161 cgtgaccctg cgccgaagaa cgggcgagcc cgagccgaac gtcatctacg cgcgcgaagt
     2221 ggagccgcaa atcaacgagc gcgagaagaa cgaggaggag gtgcgcgagc tcctcgctct
     2281 tgccgagcaa ctcgagggac tcacccgcaa cgtcggcatg cacgccggag gcgtgctgat
     2341 cgcacccgga aagctcaccg acttctgccc cctctatgcc gcggacggct cggtgagcgt
     2401 ggtgtcgcaa ttcgacatga aggacgtgga ggcggtcggg ctcgtcaagt tcgacttcct
     2461 ggggctcacc accctcacca tcctcgactg ggcggtgaag tatatcgagc aactcgaccg
     2521 cgagtccaag gtaaagctcg acgaaatccc gctcgacgac gcggaaacct atcgcgtctt
     2581 cgcgaacgcc gacacgaccg ccgtgttcca gtgtgaatcg cgcgggatgc gggagctcat
     2641 gaagcaggcg aagcccgacc gcttcgagga catcgtcgcg ctcatcgctc tttatcgtcc
     2701 gggacccatg gagctcatcc cggaattcat ccgccgcaag cacggcgggc gcatcgagta
     2761 tcccgacccc cgcgtcgagc cgatcctgaa acccacctac ggcatcatgg tgtaccagga
     2821 gcaggtgatg cagatcgcgc aggtgatcgg cggctacagc ctcggaagcg cggatctgct
     2881 gcgtcgggcg atgggcaaga agatgccgga ggagatggcg cgccaccgcg agaccttcgt
     2941 caagggcgcc gcgcagagcg gcgtcccgga gcgcaaggcg aacgagctct tcgacctcat
     3001 ggagaaattc gcgggctacg gcttcaaccg ctcgcacgca gcggcgtacg cgctggtggc
     3061 gtatcagact gcctatctca aggcgcacca cgccgccgca ttcagcgcgg ccaacatgtc
     3121 cgcggtgatg gcggacaccg acaaggttca gctttttgcg gacgatgcgc gcgagcacgg
     3181 gatcgtgctc ctgccgccgg acatcaatgc atcgaactat cgcttcgagc cgctcccgcc
     3241 gggcgagaac gggcaatgcg gggtggtccg ctacgggctc ggcgccatca agggcaccgg
     3301 cgaggcggcg atcgagcaca ttatcgacgc gcgccggggc ggcgggccgt tcaaggacct
     3361 tttcgatttc tgtcaccgcg tggacaagcg catcgtcaac cgtcgcgtcg tcgagtcgct
     3421 cattcgcgca ggggcgttag attccatcga cgatcaccgc gcgcggctcc tcgcctcggt
     3481 cggcacggcg atggaaagcg cggaacaggc aagccgcagc gcgaatcagg tgagcctgtt
     3541 cggcgagctc gcgcacgcgg gaccccctgc cgcgctcgcc gatgtcgcgc gctggagcga
     3601 tcgcgaacgg ctccagaacg agaaactcgc cctcggcttc tatctttccg gccatccttt
     3661 cagaagctac gaagaggagc tgcgccagtt cgtcaccacg cggctggacg agcttgcgcc
     3721 ccagccgtat ccggtgctgc tcgccggcat catccacagc ctccgggtgc agatgacgcg
     3781 ccgcgggcgc atggcggtaa ttctcctgga tgacggccgc gcgcgtgtgg agctcaccgt
     3841 gttcaacgag cttttcgagc agcaccgcaa cgcgctcaag gaggatcagc tgctgatcgt
     3901 cgagggcaag gtcgcgcacg acgatttcag cgggagcctg cgcatctccg cggaaaagct
     3961 ttacgatctc gcagccgctc gcaaccgcta cgcgcgcgcg atccggctga cgtgcaacgg
     4021 cgcatcatcc ggcgcgcgcc tgcgcgagct actcgctccg catcgcgacg gcagctgtcc
     4081 cgtctcgatc gtgtactcga atcatggcgc gatgtgcgaa atcgatctcg gggagaagtg
     4141 gcaggtggcg ctccacgacg aactcatccg cgcgctcggc gaatggctga agcccgagaa
     4201 tgtgagcatc gtctatgcca cgcgcgcgta aacgcagtgc ctccgtccgc gcgctgttcg
     4261 ccgctttcct cttcaccgca ttcgcaccgc ttacgcacgc cggcgaggcg atctggcggg
     4321 cgctgaacga cgagatcgtg ccgctcatca cgcgcggcga gctgcatcat gccgagcgca
     4381 cggcgcgcca agcgctggcc gaagcgcgga aaacgtttgg cgacacgcat cgcaataccg
     4441 aaacgagcct cgccagcctc gcgctcgcct tgcgctttcg cggcaagcat gaggaggctg
     4501 agacgcacta ccgcgcggca ctcgcgctcc gcgagaaaac gatcggtgcc gatcatccct
     4561 ccaccgccat gctgctcgcg aatctggcgg aggtgatcca gtcgcaacgc cgctatgcgg
     4621 aggcggagaa gatccagcgc cgcgtgcttc cgatcttcga gaaggcgtac ggcgaggatc
     4681 cgaaaaccgc gacgctgctc aataacctcg gtgcggtgct ccgcgtgcag ggccgccaca
     4741 aggaggccga gccgctgctc cgccgcgcgc tcgtcatgaa agaaaacacg ctcggaccgt
     4801 acaacccgag cgtggccaat acgctcacca atctcgccga agtgctcgac catctcggcc
     4861 gcagggacga agccgcgcag atgcgcctgc gcgctgcggc gatcacccgc taaccgttat
     4921 tcggtcggac gcgtctcgaa gcccgtaaac gcgccttccg cgtccgttac gccgacttcc
     4981 tgtacccttg cgcccggcgg cccgcggcgc gcccacgcga tgagggcatc gaccgcttcg
     5041 gctgaaccgt gcac
//
LOCUS       DASURG010000250         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1269254, whole genome shotgun sequence.
ACCESSION   DASURG010000250 DASURG010000000
VERSION     DASURG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269254"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="ilvC"
                     /locus_tag="VFY80_04285"
     CDS             <1..691
                     /gene="ilvC"
                     /locus_tag="VFY80_04285"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX6007155.1"
                     /translation="NIHFKQIEPRADLDVIMIAPKGPGHLVRSTYTQGGGVPSLIAIA
                     QDASKKARDVALSYAAAIGAARAGVIETTFREECETDLFGEQVVLCGGLTALIQAGYE
                     TLVDAGYAPEMAYFECLHEVKLIVDLIYEGGIANMRYSVSNTAEYGDFTRGPRIINEQ
                     VRAEMKKILHEIQTGQFAKEFILEHKAGAATLKAMRRIGTAHEVEAVGAKLRDMMPWI
                     KKNRLVDQSRN"
     gene            758..1453
                     /locus_tag="VFY80_04290"
     CDS             758..1453
                     /locus_tag="VFY80_04290"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX6007156.1"
                     /translation="MGSGFRRNDDPSAETDLVSRYPHPLIAREGWFYVGLSLAVAAVV
                     HAFAGFWWALPLWLVALFVVQFFRDPPRTIPDDPRAVLAPADGRIVAVERAQDPYLNR
                     DALKMSVFMNVFNAHSNRSPVDGEIRDTWYHAGKFINAMMSKASLENERAALWIRTPA
                     GEDVTCVQVAGLIARRILCYAKAGQKLARGERFGFIRFGSRVDVYLPPAAVPRASIGD
                     KVYATETVLALLP"
     gene            1463..2287
                     /gene="pssA"
                     /locus_tag="VFY80_04295"
     CDS             1463..2287
                     /gene="pssA"
                     /locus_tag="VFY80_04295"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX6007157.1"
                     /translation="MYEPRESKLFIKTRFARRGIYWLPNLFTIIALFAGFYAIVQAMS
                     GRFEHAAIAIFVAMVFDGLDGRVARLTRTQSAFGAELDSLSDMVSFGVAPALVVYVWA
                     LQDFALVDKASVLGPWLSSKLGWIAAFIYCACAALRLARFNTSLDIADKRFFQGLPSP
                     AAACLIAGLVWALNTHQINGYDVRWLAWALTMFAGLSMVSNLKFYSGKDINLRKSVPF
                     SVVVGIAMGLVFLVSFQSTLPEMLFALFTCYGLSGYVIWLWDAMKGRSAKPPSAAH"
     gene            2533..>3680
                     /locus_tag="VFY80_04300"
     CDS             2533..>3680
                     /locus_tag="VFY80_04300"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6007158.1"
                     /translation="MAQEKLIIFDTTMRDGEQSPGASMTKEEKLRIARQLERMRVDVI
                     EAGFPAASDGDFDAVQAVARIVKESTVCGLARANENDIRRCGEAVRVAASPRVHTFIA
                     TSPIHMEKKLRMEPSQVIEQAVRAVKWARDYTDNIEFSPEDAGRSDIDFLCRILERVI
                     KAGATTINIPDTVGYTLPDAFGGLIRTLRERIPDSDKVIWSVHCHNDLGLAVANSLSA
                     VLSGARQVECTINGLGERAGNAALEEIVMAVRTRQDVFACDTGIDATQIVPASRLVSG
                     ITGFPVQPNKAIVGANAFAHESGIHQDGVLKSRDTYEIMRAQDVGWVANKLVLGKHSG
                     RNAFKTRLQELGIVIDSEEMLNAAFKRFKDLADKKHEIFDEDLQALVT"
BASE COUNT          650 a         1175 c         1188 g          667 t
ORIGIN      
        1 caacatccac ttcaagcaga tcgagccgcg tgccgatctc gatgtcatca tgatcgcgcc
       61 caaggggcca ggccacctcg tccgttccac gtacacgcag ggtggcgggg tgccttcgct
      121 catcgcgatc gcgcaggatg cgtcgaagaa ggcgcgcgat gtggcgctct cctatgcggc
      181 cgcaattggc gcggcgcgcg cgggggtcat cgaaaccacc ttccgcgaag agtgcgagac
      241 cgaccttttc ggcgagcagg tggtgctctg cgggggcttg accgcgctca tccaggccgg
      301 gtacgagacg ctggtcgatg cgggttatgc gccggaaatg gcgtacttcg agtgcctgca
      361 cgaagtgaag ctcatcgtgg acctcatcta cgagggcggc atcgccaata tgcgctattc
      421 ggtctcgaac accgcagagt acggcgactt cacccgcggg ccgcgcatca tcaacgagca
      481 ggtgcgggcc gagatgaaga agatcctcca cgagatccag accggccagt ttgcaaagga
      541 attcatcctg gagcacaagg caggcgccgc gacgttgaag gcgatgcgcc gcatcggcac
      601 ggcacatgag gtcgaagcgg tgggcgcgaa gctgcgcgac atgatgccgt ggatcaagaa
      661 gaaccgcctg gtggaccagt cccggaatta ggttgcgctt atcacatcac tgcgacgata
      721 ttcgtcttgc cggtgaaaac cggcatccag cgtgcgcctg ggttccggct ttcgccggaa
      781 tgacgacccg tcggcggaaa ccgacctcgt gagccgttat ccccatcccc tgatcgcccg
      841 cgaaggctgg ttttacgtcg ggttgtcgct cgcagttgct gcagtcgtcc atgcatttgc
      901 cgggttctgg tgggcgctgc cgttgtggct cgtcgcgctc tttgtcgtcc agttcttccg
      961 cgatccgccg cgcacgattc cggatgaccc gcgcgccgtg ctcgcaccgg cggacggcag
     1021 aatcgttgcg gtcgagcgcg cgcaggatcc gtatctcaat cgcgatgcgc tcaagatgag
     1081 cgtcttcatg aacgtcttca acgcgcactc gaaccgcagc cccgtggacg gcgagatccg
     1141 cgacacctgg tatcacgccg gcaagttcat caacgccatg atgtcgaaag cctcgctcga
     1201 gaacgagcgc gccgcgctgt ggatccgcac cccggcggga gaggacgtga cctgcgttca
     1261 ggtggcgggg ctcatcgcgc ggcgcatact gtgctatgcg aaagccgggc aaaagctcgc
     1321 gcgcggcgag cgcttcggct tcatccgctt cggctcgcgc gtggatgtct atctgccgcc
     1381 tgctgcggtg ccccgcgcga gcattggcga taaggtctac gccaccgaaa cggtgctcgc
     1441 gcttctgccc tgagcccgga cgatgtacga gccgcgcgaa agcaagctct tcatcaaaac
     1501 ccggtttgcg cggcgcggga tctactggct ccccaatctc ttcaccatca ttgcgctgtt
     1561 cgccggcttc tacgcgatcg tgcaggcgat gagcgggcgc ttcgagcacg ccgcgatcgc
     1621 gattttcgtc gccatggtgt tcgacgggct cgatggccgc gtcgcgcggc tgacgcgcac
     1681 gcagagcgcg ttcggtgcgg agctcgacag cctctccgac atggtctcgt tcggcgtggc
     1741 acccgctctc gtcgtctacg tgtgggcctt gcaggacttt gcgctggtgg acaaagcgtc
     1801 ggtgctcggc ccgtggcttt cgagcaagct cggctggatc gcggcgttca tctactgcgc
     1861 gtgcgccgcg ctgcgccttg cgcgcttcaa cacgtcgctc gacatcgccg acaagcgctt
     1921 cttccagggc ttgccgagtc ccgccgcggc gtgcctcatc gccggactcg tgtgggcgct
     1981 caacactcac cagatcaacg gctacgacgt gcgctggctc gcatgggccc tcacgatgtt
     2041 cgccgggctc tccatggtga gcaatctcaa gttctacagc ggcaaggaca tcaacctgcg
     2101 gaagagcgtg ccgttctcgg tggtggtcgg catcgcgatg gggctcgtgt tcctcgtgag
     2161 cttccagagc acgctgcccg agatgctgtt cgcgctcttc acctgctacg ggctttcggg
     2221 ttacgtgatc tggctttggg acgcgatgaa ggggcgctcg gcgaagccgc cttctgcggc
     2281 ccactagggc cttgcaccgg ttcgcaaaac acactatatt tccgcgacat gtatccgatc
     2341 agccaggcca atgccgcact cctcaccgcc agcctcctgc tggccggcct gctgctgcgc
     2401 gttcgcgcgc gctaatactc tacccccact ttcgcagtta tcgtccggag cggccagccg
     2461 cccggagttc atttttgatc gaatcctgcc cgaggcgacg cggcaggcgc agcagagaga
     2521 gcggagaaca tcatggccca ggaaaaattg atcatattcg ataccaccat gcgcgacggc
     2581 gaacagagcc cgggcgcctc gatgacgaag gaagagaaac tgcgcattgc gcgccagctc
     2641 gagcgcatgc gcgtggacgt gatcgaggcc ggcttcccgg cggcgagcga cggcgacttc
     2701 gacgcggtgc aggcggtcgc gcgcatcgtg aaggagagca cggtgtgcgg gcttgcgcgc
     2761 gccaacgaga acgacatccg gcgttgcggc gaagcggtgc gggtggccgc ttcaccgcgc
     2821 gtccacacct tcatcgcgac ctctcccatc cacatggaga agaagctccg catggagccc
     2881 tcgcaggtga tcgaacaggc ggtgcgcgcg gtcaaatggg cgcgcgacta taccgacaac
     2941 atcgagtttt caccggagga cgcgggccgc tcggacatcg attttctgtg tcgcatcctc
     3001 gagcgcgtga tcaaggccgg ggcgactacg atcaatattc ccgatacggt cggctacacg
     3061 cttcccgacg ccttcggcgg cctcatccgc acgctgcgcg agcgcattcc cgattcggac
     3121 aaggtgatct ggtccgtgca ctgccacaac gatctcggcc tcgcggtggc caattcgctc
     3181 tccgccgtgc tgagcggggc gcggcaggtc gagtgcacca tcaacgggct gggcgagcgt
     3241 gccggcaatg cggcgttgga agagatcgtc atggcggtgc gcacgcggca ggacgtgttt
     3301 gcctgcgaca ccggaatcga cgcgacgcag atcgtcccgg cgagccggct cgtctccggc
     3361 atcaccgggt tcccggtaca gcccaacaag gcgatagtcg gggcgaacgc attcgcgcac
     3421 gagtcgggca tccaccagga tggggtgctc aagagccgcg atacctacga gatcatgcgc
     3481 gcgcaggacg tgggctgggt cgcgaacaag ctcgtgctcg ggaagcacag cgggcgcaac
     3541 gcattcaaga cgcggctcca ggagctgggc atcgtgatcg actccgaaga gatgctgaat
     3601 gccgcattca agcgcttcaa ggatctcgcg gacaagaagc acgagatctt cgacgaagat
     3661 ctgcaggcgc tggtgaccga
//
LOCUS       DASURG010000251         4460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_758507, whole genome shotgun sequence.
ACCESSION   DASURG010000251 DASURG010000000
VERSION     DASURG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758507"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFY80_04305"
     CDS             <1..632
                     /locus_tag="VFY80_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX6007159.1"
                     /translation="VTLMDPSRFDVRGDLACDKDVQIDVNCVFEGKVILGAGAEVGAN
                     CVLRNVEVGSGTRIEPFTHIDDAIIGAHCRIGPFARIRPATRLAEEVHIGNFVEVKAS
                     EIAARSKANHLAYVGDTRVGHDVNIGAGTITCNYDGANKHRTVIEDEVHIGSDVQLVA
                     PVRLAKGVTVGAGTTVWRDTPQGGLIINPKTQEHRAAWKRPKKKGPEGQ"
     gene            691..2535
                     /gene="glmS"
                     /locus_tag="VFY80_04310"
     CDS             691..2535
                     /gene="glmS"
                     /locus_tag="VFY80_04310"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6007160.1"
                     /translation="MCGIVGAVAERDIVPVLLEGLKRLEYRGYDSAGIAVLDCAPASR
                     PLLRRLRSAGRVSELCSLAGEQRLSAPLGIAHTRWATHGVPSERNAHPHISGGIAVVH
                     NGIIENHEAIRLRLEAGGYRFESDTDTEVIAHLIHAHLNEGEDLLEATRASAAELQGA
                     YAIAVIADKSSGCMVIARHGAPLLLGVGDHEMFAASDVSALLPVTRSVIFLEDGDVAE
                     VRADAYRIVDSAGDPVERPIHVSELSASAVELGPYRHYMQKEIFEQPGAVAATLETVT
                     SGSTVLPNIFGVDAERVFRDAKSVLILACGTSYHAGMVARYWLEAMAAIPCAVEIASE
                     YRYRESVPDANTLVVTISQSGETADTLAALQHARSLGQQHSLTICNVPESALVRASAL
                     RFLTRAGPEIGVASTKAFTTQLAALLLLTLVIARIRGRISADDERALISALRHLPAAL
                     SRVLEVEAAVQGWAEVFAGHPHALFLGRGIHYPIAMEGALKLKEISYIHAEAYPAGEL
                     KHGPLALVDRHMPVVAIAPQDALLEKLKSNLQEVRARGGELYVLADHGSRIEAAEGLH
                     VIRLTDHAGPLSPILHVIPLQLLAYHCALYRGTDVDKPRNLAKSVTVE"
     gene            complement(2549..3211)
                     /locus_tag="VFY80_04315"
     CDS             complement(2549..3211)
                     /locus_tag="VFY80_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX6007161.1"
                     /translation="MILAGAARRAAQAALACLLLLQPAVRAADEAPRIRANVDYRLIE
                     PQPVTTGSRVEVIDFFWYGCPHCYTFQPALESWIRRMPADVALRRVPALLRDNWAPHA
                     RIYYTLEALGEVERLHQAVYHGYHVEELHMSKPDVMLQWAMRQGIDGRRWTEAYHSAK
                     VTEKVERAKEMTNDYNVQGTPSLVVDGRYLTSPAMARGEHRMIPILEELIALARAERS
                     RK"
     gene            complement(3208..3873)
                     /locus_tag="VFY80_04320"
     CDS             complement(3208..3873)
                     /locus_tag="VFY80_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX6007162.1"
                     /translation="MTFHRTIAVLTAALALFFSSAAVTAQVVAGRDYQPLSNPQPVSS
                     GARIEVIEFFWYGCPHCNNLQGPLNGWLKRKPADVEFKRMPAVFQDSWVPLTKAYFAL
                     EALGLVDRLHHDVFTAIHEKKLRLQDPNLLFDWVAKQGVDREKFAQTYNSFAVQTRAQ
                     RAREITARYDIPGTPAVVVDGKYLTAPSLILNPDKSINYDRYFRVLEEVIALARKARG
                     GKS"
     gene            complement(3870..>4460)
                     /locus_tag="VFY80_04325"
     CDS             complement(3870..>4460)
                     /locus_tag="VFY80_04325"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6007163.1"
                     /translation="ERRSSTRGGGHTLLVGILIGLVLGLGIALGVAWYINKVPSPFLA
                     RTPPSAPADKPIHGAVRSDDKTARGTETKPRFDFYKILPGEEPATDQQLKDAQRNSRA
                     AKEAFFLQAGAFQSASDADGLKARLALIGVEATIQTTSVPDKGVWHRVRIGPYTSVEQ
                     LNQARETLKHNGVDTTLIRVREATSSGADSRSMGKR"
BASE COUNT          801 a         1357 c         1448 g          854 t
ORIGIN      
        1 gcgttacttt gatggatcct tcgcgattcg acgtgcgcgg cgatctcgcc tgcgacaagg
       61 atgtgcaaat cgatgtcaac tgtgtcttcg aaggaaaagt gatcctgggc gcgggcgccg
      121 aggtcggcgc gaactgcgtg ctgcgcaacg tggaagttgg ctcgggcacg cgcatcgagc
      181 ctttcacgca tatcgacgac gccatcatcg gagcgcattg tcgcatcggc ccgtttgcac
      241 gcatccgacc ggcgactcgc ctggcggaag aggtgcacat cgggaacttc gtcgaggtca
      301 aggcgagcga gatcgcggcc cgttccaagg ctaatcacct cgcctacgta ggcgacaccc
      361 gcgtggggca tgacgtcaac atcggcgccg gtacgatcac atgcaactac gatggcgcca
      421 acaagcatcg aaccgtgatc gaagacgagg tgcacatcgg atccgatgtg cagcttgtcg
      481 caccggtccg gctcgcaaag ggtgtgacgg tgggcgccgg caccacagtc tggagagaca
      541 cgccccaagg cggactcatc atcaacccga agactcagga gcatagagcc gcgtggaagc
      601 gcccgaaaaa aaagggcccc gaaggccagt gagtgtcagg ttgagcgtcg caggtaggtc
      661 gatttctcac tgcacgggag ggcgcagccc atgtgcggca tagtcggcgc ggttgccgag
      721 cgcgacattg tcccggtgtt gctggaaggg ctgaagcgac tcgagtaccg gggttatgac
      781 tccgccggca ttgccgtact ggactgcgcg ccggccagcc ggccgctatt gcgtcggctg
      841 cgcagtgcgg gacgggtgtc ggagctctgc agcctcgcag gtgagcagcg cctgtcagcg
      901 ccgctcggaa tagcgcatac gcggtgggcg acgcacggtg taccgtccga gcgcaatgcc
      961 catccccaca tctccggcgg catcgcggtg gtgcacaacg gcatcatcga aaaccacgag
     1021 gcgatacgcc tgcggctcga agcaggcggc tatcggttcg agtcggatac ggataccgag
     1081 gtcatcgccc atctgatcca tgcgcacctc aacgagggcg aggatctcct cgaagccacc
     1141 cgcgcgagcg ccgcggagtt gcagggtgcc tatgcgatcg ccgtcatcgc cgataagagc
     1201 agcggatgca tggtaatcgc gcgccatggg gctccgctgc tcctcggtgt cggcgatcac
     1261 gagatgttcg cagcgtccga cgtgtcggcg ctgttgcccg tcacccgctc ggtgatcttt
     1321 ctcgaggatg gagatgtcgc ggaggtgcgc gcggatgcgt atcgaatcgt ggattcggcg
     1381 ggcgaccctg ttgagcgccc gatccacgtg agcgagcttt ctgcttcggc ggtcgagttg
     1441 gggccgtatc ggcactacat gcaaaaagag attttcgagc agccaggcgc cgttgcagcg
     1501 acgctcgaaa cggtaacgag cggctcgacc gtgctgccga acatttttgg cgtggatgcg
     1561 gagcgggtgt ttcgcgacgc gaagagcgtg cttatcctgg cgtgtggcac gagctatcac
     1621 gccggcatgg tggcgcgcta ttggctcgaa gcgatggcag cgattccgtg cgctgtggaa
     1681 atcgcgagcg aatatcgcta tcgcgagtca gttccggatg ccaatacatt ggtggtgacg
     1741 atttcgcaat cgggggaaac ggcggacacg cttgcggcgc tccagcacgc tcgttcgctg
     1801 ggacagcagc acagcctgac gatctgcaat gtgcctgagt ctgcgctggt gcgcgcttcg
     1861 gcgctgcgtt tcctcacgcg agcgggcccc gaaatcgggg tcgcatcgac caaggccttc
     1921 acgactcagc tcgctgcact gctcctgctt acgctcgtta tcgcacgcat tcggggccga
     1981 atatcagcgg acgacgagcg ggcattgatc agcgcattgc gacaccttcc cgccgcgctc
     2041 tcgcgcgtcc tggaagtcga agcggccgtg cagggctggg cagaggtttt cgctggccac
     2101 ccgcacgcgc tctttctcgg acgcggcatc cattacccga tcgccatgga gggcgcgctc
     2161 aagctcaagg aaatttctta catccatgcg gaggcctatc ccgctgggga gctcaagcat
     2221 ggtccccttg cgctcgtgga ccggcatatg ccggtggttg cgattgcgcc acaggatgcg
     2281 ctgctggaga agctcaaatc gaatcttcag gaagtgcgcg cccggggcgg cgaactgtat
     2341 gtcctcgccg atcacggctc gcgcatcgag gcagcagagg ggctgcatgt aatccgcctg
     2401 accgaccacg ccggcccgct ctcccccatt ctgcacgtga taccgctgca gcttcttgcc
     2461 taccactgcg cgctttatcg cggcaccgat gtggacaagc ctcgcaatct cgccaaatcc
     2521 gtgacggtcg agtaactgac tcccgtggtt actttcggct gcgctcggct cgcgctagcg
     2581 cgatcagctc ttccaatatc gggatcatgc ggtgttcgcc gcgcgccatc gcaggcgaag
     2641 tgagatagcg cccatcgacg acgagcgaag gggtcccctg gacgttgtag tcgttcgtca
     2701 tttcctttgc gcgctcgacc ttctcggtca ccttggccga gtggtaggct tcagtccagc
     2761 gcctgccgtc gatgccctga cgcattgccc actgcagcat gacgtccggt ttgctcatgt
     2821 gcagttcctc tacgtgatag ccgtgataca ccgcctggtg caggcgctca acctctccca
     2881 atgcttcaag cgtgtagtaa atacgcgcgt ggggagccca gttgtcgcgc aggagtgcgg
     2941 gcacgcggcg cagcgcgacg tctgctggca tgcgcctgat ccagctctcg agtgcgggct
     3001 ggaaggtgta gcagtgcggg cagccgtacc agaagaaatc gatgacttcg acgcgtgatc
     3061 ctgtcgtcac cggctgcggc tcgatcaacc gatagtccac gtttgcccga atgcgtggag
     3121 cttcgtccgc agcccgcacg gcgggttgca gcaggagcag gcaagcgagc gcggcctgcg
     3181 ccgcgcggcg tgccgcccca gcgagaatca tgacttgccg ccccgggctt tgcgtgcgag
     3241 cgcaatgact tcttccagca cccggaaata acggtcgtaa ttgatgcttt tatcggggtt
     3301 gagaatgagt gagggcgccg tcaggtattt cccgtccacc accacggcgg gtgtgccagg
     3361 aatgtcgtat cgtgcggtaa tctcgcgggc gcgctgcgcg cgggtttgca ccgcgaagga
     3421 attgtaggtc tgcgcaaact tctcgcggtc cacgccctgc ttcgccaccc agtcgaacag
     3481 taggttgggg tcctgcaggc gaagcttttt ttcgtgtatg gcggtgaaca cgtcgtgatg
     3541 caatcgatcg acgaggccga gcgcttcgag ggcgaagtaa gccttggtca gcgggaccca
     3601 ggagtcctgg aaaacggccg gcatgcgctt gaactctacg tcggcgggct tgcgcttcaa
     3661 ccacccgttc agcggaccct gcaggttgtt gcagtgcgga cagccgtacc agaagaactc
     3721 gattacttcg atgcgcgcgc cgcttgaaac gggctgggga ttggacaatg gctggtagtc
     3781 ccgccctgca acgacctgcg ccgttacagc ggcagacgag aagaaaagtg cgagcgccgc
     3841 ggtcagcacg gcgatagtcc tgtggaatgt catcgtttcc ccattgaacg agaatctgct
     3901 cccgagctcg tcgcctcccg caccctgatc aaagtggtgt cgacgccgtt gtgcttcagc
     3961 gtctcgcggg cctgattgag ctgctcgacg ctcgtgtacg ggccgatgcg aacgcgatgc
     4021 cacacaccct tgtccggaac cgaagtcgtc tggatcgttg cctcgacccc aattaaggcg
     4081 aggcgcgcct tgagaccatc ggcgtccgac gcgctctgaa aggcgcccgc ctgaagaaag
     4141 aaagcttctt tcgcggcgcg cgaattccgc tgcgcgtcct tcagctgctg gtcagtggcc
     4201 ggctcctcgc ccggaagaat tttgtagaaa tcgaagcgcg gtttggtttc agtcccgcgg
     4261 gcggtcttgt cgtcgctgcg cacggcgccg tggatcggct tgtctgccgg cgcggagggc
     4321 ggtgtacggg caaggaacgg actgggcact ttgttgatat accaggcaac tccaagcgca
     4381 atgccgaggc cgagcacaag gccgatcagt atgccgacga gcagcgtgtg gccgccgccg
     4441 cgcgtgctgc ttctgcgctc
//
LOCUS       DASURG010000252         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_963884, whole genome shotgun sequence.
ACCESSION   DASURG010000252 DASURG010000000
VERSION     DASURG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963884"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFY80_04330"
     CDS             complement(<1..335)
                     /locus_tag="VFY80_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6007164.1"
                     /translation="MQVTSMHFKARAGGKLADVKLQAALKKLQTNFVRGRAERVAELD
                     NFQAIRDAGAAIRDRALVNLDLYLAEFERNATKAGALVHWAETTAEANRIVCEIAAQH
                     GVRKIVKSKS"
     gene            complement(353..1447)
                     /locus_tag="VFY80_04335"
     CDS             complement(353..1447)
                     /locus_tag="VFY80_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6007165.1"
                     /translation="MSVPRLTVRALHTQAVKVPMTYALGTSAATVREAPLMLFDLETE
                     EGVTGHAYLFCYLPAAAPAIASFAHEVERAVKGDRVAPLDLWAKLAKRFTLIGVQGIV
                     RMAMAALDVAAWDALAKAAGLPLAEYLGGTVRPIRAYNSCGLGLMAPEEVADEAEKLL
                     ANGFRAMKLRLGYPTLEADLAALHALRKRVPEDIEIMVDFNQALTVAEALRRGRALDT
                     EGVYWIEEPIRHDDYAGAAILARELATPVQIGENFSLPRAMLEALDVKACDYVMPDLE
                     RIGGVTGWRQAAGIAAARGIEMSSHLFPEVSAHLLAVTPTRHWLEYVDWANAVVREPL
                     VVVAGEAVVPDRPGNGLEWNAKAVVRYRMQ"
     gene            complement(1449..2375)
                     /locus_tag="VFY80_04340"
     CDS             complement(1449..2375)
                     /locus_tag="VFY80_04340"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX6007166.1"
                     /translation="MKIAIMGSGGVGGYFGARLVQGGCDVHFIARGAHFAAMREHGLK
                     VESGLGEALIKPVKVTDDPRTLPKVDLVFFSVKMWDTESAARSIASLFGPDTGIVSFQ
                     NGVQKDEILRGVFGEKAVMGGVGYIATSIARPGVIKHLGTLQRLVFGEYDGSRSARAE
                     ALLAACRKGGIDAELSSDIRRQIWEKFVFLVGLSGTTATTRRPIGAVRANPRTRGFLL
                     DLMREVVAVGRAQGVALDADYAEKRLAFCDTLPEDMTSSMHHDLERGNPLEVEWLSGD
                     VVRMGEKFDIPTPANRAVRDILTLHAEGRRSG"
     gene            complement(2458..3189)
                     /locus_tag="VFY80_04345"
     CDS             complement(2458..3189)
                     /locus_tag="VFY80_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6007167.1"
                     /translation="MRVGLFVTCLVDLVRPRIGFAALELLEAAGCEVIVPRMQTCCGQ
                     PGYNSGDRQTALALARKVLREFEDCDYVVAPSGSCAGMIRTHYLDLFSGDPERERAAQ
                     LCERTYELTDFLVNVARLEHVAGRFAGSITYHDSCSGLRELGVKAQPRALLAKVDGLT
                     VKEMEEAEVCCGFGGAFAIKFGDISSHIADRKCENIAAAGADAVVLGDLGCMLNIEGR
                     LRRRGDMKTKVLHVAEVLTGSHEES"
     gene            complement(3455..>4230)
                     /locus_tag="VFY80_04350"
     CDS             complement(3455..>4230)
                     /locus_tag="VFY80_04350"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX6007168.1"
                     /translation="VNRKKDLERRPWWHKASLEGTPQLSDPTMLKFRAEGSRMPDQTD
                     AQLAEMTANYYGMISLIDHNVGRILTALDELSLARDTLVIYTTDHGEMLGNHGLYLKG
                     PTPYEDLLRVTLLVRGRDVASGVNIVEPVSTLDLAPTMYDYAGCSSPRSLQGRSLKRL
                     VSGVPETREAAYSEWHVHPSRCGVALKLRTIRTRAHKCTFELESGAGELYDLANDPGE
                     MRNLFDDPSYSGLRKQFEELMRARPGNYREDLPEPIGMA"
BASE COUNT          782 a         1387 c         1303 g          758 t
ORIGIN      
        1 gactttgatt tgacgatctt gcgcacgccg tgctgcgcgg cgatctcgca cacgatgcga
       61 ttcgcctcgg ccgtcgtctc cgcccaatgc accagcgcgc ccgctttggt cgcattgcgc
      121 tcgaattccg cgagatagag atcgaggttg acgagcgcgc ggtcgcggat cgccgccccg
      181 gcatcgcgga tcgcctggaa attgtcgagt tccgccacgc gttcggcgcg tccccgcacg
      241 aaattcgtct gcagcttctt gagcgcggcc tggagcttca catcggcgag cttcccgcca
      301 gcgcgcgcct tgaaatgcat cgaggtgact tgcatgggtt gcgcgcgccg ctctactgca
      361 tgcgataccg gacgaccgct tttgcgttcc actcgagccc gtttccggga cgatccggga
      421 cgacggcctc tcctgccacg accacgagcg gttcccgcac gaccgcattc gcccagtcca
      481 cgtattcgag ccagtggcgc gtgggcgtca cggcaaggag atgtgcgctc acttcgggaa
      541 aaagatgcga ggacatctcg atgccgcgtg ccgccgcgat cccggccgcc tgacgccagc
      601 cggtcacgcc gccgatgcgc tcgagatcgg gcatgacgta atcgcatgcc ttaacatcga
      661 gggcctccag catcgcgcga ggcagcgaga agttctcgcc aatctgcacc ggcgtcgcga
      721 gttcgcgggc gagaatggcg gcgcccgcat agtcgtcgtg ccggatcggc tcttcgatcc
      781 agtacacgcc ttcggtatcc agtgcgcgcc cgcggcgcag ggcttccgcg accgtcagcg
      841 cctggttgaa atccaccatg atctcgatat cctccggtac gcgctttcgc aaggcatgga
      901 gtgcagcgag atccgcttcg agggtcgggt aaccgagccg caatttcatc gcgcgaaagc
      961 cgttggcgag aagcttttcc gcttcgtccg cgacttcttc gggcgccatg aggccgagcc
     1021 cacaactgtt gtaggcgcgg atgggtcgaa ccgttccgcc gagatattcg gccagcggca
     1081 ggcccgcggc tttggcgagc gcatcccatg ctgcgacgtc gagcgccgcc attgccatgc
     1141 gcacgatacc ctgcacaccg atgagcgtga agcgcttcgc gagcttggcc cacagatcga
     1201 gcggtgccac gcgatcgccc ttcaccgcgc gctccacttc gtgcgcgaag ctcgcgatcg
     1261 caggcgcggc ggcaggcaga taacagaaga gatacgcgtg tccggtgacg ccttcctcgg
     1321 tctcaagatc gaacagcatg agcggcgctt cacgcacggt cgccgcactc gttccgagag
     1381 cgtaggtcat cggcactttg accgcctgcg tatgcagtgc gcgcaccgta agccgaggca
     1441 cgctcacgtc agccgctccg tcgcccttca gcgtgcagcg tcaggatatc gcgcaccgcg
     1501 cggttggccg gcgtcgggat gtcgaatttt tcgcccatgc gcaccacatc gccgcttagc
     1561 cactccactt cgagcggatt gccgcgctcg agatcgtggt gcatcgaaga ggtcatgtct
     1621 tccggcagtg tgtcgcaaaa cgcgaggcgc ttttccgcat aatcggcgtc cagcgccacg
     1681 ccctgggcgc gcccgacggc gaccacttcg cgcatgagat cgagaagaaa gccgcgcgtg
     1741 cgcggattgg cgcggaccgc gccgatcggc cggcgggtcg tcgcggtcgt gccggaaaga
     1801 ccgaccaaga agacgaactt ttcccagatc tggcgacgga tgtcgctgct caattccgca
     1861 tcgatgccgc ctttccggca ggccgcgagg agcgcctcgg cgcgtgctga acgcgagccg
     1921 tcatattcac cgaatacgag ccgctgcagc gtgcccagat gcttgatgac gcccggacgc
     1981 gcgatcgacg tggcgatgta gcccacgccg cccatcaccg ctttttcgcc gaagacgccg
     2041 cgtaggattt cgtccttctg cacgccgttc tgaaacgaca cgatgccggt atcgggcccg
     2101 aaaagcgaag cgatcgagcg cgcggccgat tcggtatccc acatcttcac cgagaagaag
     2161 acgagatcga ctttggggag cgtccgcgga tcgtccgtga ccttcacggg cttgatcagc
     2221 gcctcgccga gtccgctctc gactttgagc ccgtgctcgc gcatcgcggc gaaatgcgca
     2281 ccgcgagcga tgaagtgcac gtcacatccg ccctgcacga gccgggcgcc gaaatagccg
     2341 ccgacgccgc ctgaacccat gatcgcaatc ttcatttcat ccttccatca ttccggcgac
     2401 attaagcatg cttcggaaag ctttgatctc gggccggaat ccactgcagt aacgcgttca
     2461 gctctcctca tgagaacccg tcagcacttc agccacgtgc aacactttgg ttttcatatc
     2521 cccccgccgt ctgaggcgtc cctcaatgtt gagcatgcat ccaaggtcac ccaggacgac
     2581 ggcatcggca ccggcagccg cgatgttttc acacttgcga tccgcgatgt gcgatgagat
     2641 gtcgccgaac ttgatcgcaa acgcgccgcc gaagccgcag cacacctcgg cttcctccat
     2701 ctccttcacc gtgagcccgt cgacttttgc gagcaacgcg cgcggctggg ctttgacgcc
     2761 aagctcgcgg aggcccgagc acgaatcatg atacgtgatg ctgccggcga agcggcccgc
     2821 aacgtgctcg agccgcgcga cgttgaccag aaaatcggtc aactcatagg ttcgctcgca
     2881 gagttgcgca gcacgctccc gctcggggtc tccggaaaag agatcgaggt agtgcgtgcg
     2941 gatcatcccc gcgcacgacc ctgagggcgc gacgacataa tcgcaatcct cgaactcgcg
     3001 caagaccttg cgcgcgagtg cgagcgccgt ttgccggtcg cccgaattgt atccgggctg
     3061 cccgcagcag gtctgcatcc tgggcacaat cacttcgcag cccgccgctt cgagcaactc
     3121 gagtgcggca aaaccgatgc gcgggcgcac cagatcgacg aggcaggtaa cgaagaggcc
     3181 tacgcgcatg ttggagactt gggaaagcag cccgcattat tgcagaaaaa agggcgcacc
     3241 tttggcgtca gcggaatcga atgccaagag atccgccgga gtcgctcggg cgaaagccgg
     3301 atctcgtgga gtcactgatg cctggatccc ggctttcgcc gggaagacga ggaacggaga
     3361 ctacagcgtg ccggaaaacg aaaccccgaa tgccgggaag acgagacttc agcaatggcg
     3421 ggaaggcgaa acgccgaagg ccgcgaagaa gtcgtcacgc catgccgatc ggttcgggca
     3481 ggtcctcgcg atagtttccc ggccgcgcac gcataagttc ctcgaactgc tttctcaagc
     3541 cggaatacga cggatcgtcg aacagattgc gcatctcgcc cggatcgttc gcgagatcgt
     3601 agagctcgcc cgcgcccgat tccagttcga aggtgcactt gtgcgcgcgg gtgcggatgg
     3661 tgcgcagctt gagcgcgacg ccgcagcgcg aagggtgtac gtgccactcg ctgtacgcag
     3721 cttcgcgcgt ttccggcacg ccggatacga ggcgcttcaa actgcggccc tgcagcgaac
     3781 gcggtgagct gcagcccgcg tagtcgtaca tcgtcggagc gagatcgagc gtcgagaccg
     3841 gctcgacaat attcaccccg ctcgccacat cgcgaccgcg cacgaggagc gtcacgcgca
     3901 gcaagtcctc gtacggcgtc gggcccttga gatagagtcc gtggtttccc agcatctcgc
     3961 cgtgatcagt ggtgtaaatc acaagcgtgt cgcgcgcgag gcttagctcg tcgagcgcgg
     4021 tcagtatccg cccaacgttg tgatcgatga gcgagatcat gccgtaatag ttcgcggtca
     4081 tctcggcgag ctgcgcgtcg gtctgatccg gcatgcgtga gccctcggcg cggaacttga
     4141 gcatggtcgg atcggaaagc tgcggcgtgc cttcgagcga tgccttgtgc caccacggac
     4201 gccgttcgag atccttcttc cggttcaccg
//
LOCUS       DASURG010000253         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_657488, whole genome shotgun sequence.
ACCESSION   DASURG010000253 DASURG010000000
VERSION     DASURG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657488"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFY80_04355"
     CDS             complement(<1..654)
                     /locus_tag="VFY80_04355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6007169.1"
                     /translation="MPIFDLHAHWFPQPWIDLLEREAHLNGARITRNDKGHAVIDIEG
                     VALKSVFRPDMIEPAIMLANMDAAKVDVRAMSLTNPMVYWAAPAFGLKLSQAYNDASA
                     ALHTAHPERFLGTIMLPLQAPELALQELDRAAKLPGMRGVYMAMHVGGKNVDDKPLWP
                     VYRRCEELALPVLMHPVNPLGIERMRRFHLRNLCGNPYEAGIAAASLIFGGVLDAFPK
                     "
     gene            complement(665..1696)
                     /locus_tag="VFY80_04360"
     CDS             complement(665..1696)
                     /locus_tag="VFY80_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6007170.1"
                     /translation="MNESGEGAARHPGGVAVIDTHAHWYPRAFIALLERKAAEHGARM
                     VRNARGVPVFSLPGVQPVSAMPPLMVEPELILAEMDRRGIATFVLSLTNPMVYWAPPG
                     FGLELAQTWNDASAAVHQAHPTRFIGTIQLPMQAPELAVGELERAAKLPGMRVVYLAT
                     HINGSNLDDERFWPVYERCEALGLPIFLHPLYPCGGDRIAAHFMRNLCGNTYENGLAA
                     ASLVFGGVMDAFPRLDVMLPQAGGTFPWLIGRFDRGVAVRKELAHMKRPASAYLRRFH
                     YDTVSHHPRIMRFLIELVGADRIVLGSDYNQDMSCERPVEFVREVPALSEEERRLILG
                     GNARRLMGL"
     gene            complement(1693..>2893)
                     /gene="tcuA"
                     /locus_tag="VFY80_04365"
     CDS             complement(1693..>2893)
                     /gene="tcuA"
                     /locus_tag="VFY80_04365"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6007171.1"
                     /translation="RTDPDLSETLITKSFEGMVWLRSQGAKFIPNYGSQSAIVNGKRK
                     FFGRFPITVNGGGAALVEELTRTAVKKGIEIFYETRATSLVYDGVRVLGVRAKQRGEP
                     VEFRARAVVLACGGFEANPEWRTRYLGAGWELAKVRGTRHNMGEGLRMALEIGACPYG
                     NWSGRHAVSWERHAPEFGVVERSHDPYRHSYPYSVMINAEGKRFVDEGADFYNYTYAK
                     YGAEVLKQTGQYAYQLFDAKVKHLLKQEYSGRNVTRFTANTLEELAAKMEGVDARQFL
                     KTIREYNTAVRSDVPFNHAIRDGRCTVGIDPPKSNWANPLDTPPFEAYGVTCGITFTF
                     GGLRINHETGQVLDLGYAPIPGLYAAGEMVGGIFYFNYPAGTGLVSGLVFGRIAGNGA
                     AAAVKAQ"
BASE COUNT          502 a          962 c          921 g          508 t
ORIGIN      
        1 cttcggaaac gcatcgagca cgccgccgaa gatgagggag gcggccgcaa tgcccgcttc
       61 gtacggattg ccgcacaggt tgcgcaggtg gaacctgcgc atgcgctcga tgccgagtgg
      121 attcaccggg tgcatgagaa ccggcagggc aagctcctcg cagcgccgat agaccggcca
      181 cagcggtttg tcgtccacgt tcttgccgcc cacgtgcatc gccatgtaga cgccacgcat
      241 gcccggcagc ttcgccgcgc ggtcaagttc ctgcagggcg agctccgggg cctgaagcgg
      301 cagcatgatc gtcccgagaa aacgctccgg atgggcggta tgcagcgctg ccgatgcatc
      361 gttgtatgct tgcgagagct tcaacccgaa tgccggcgct gcccagtaca ccatcgggtt
      421 ggtgagcgac atggcccgca catccacctt cgcagcatcc atgttcgcaa gcatgatggc
      481 gggctcgatc atgtccgggc ggaacacgga cttgagcgca acgccctcga tgtcgatcac
      541 cgcgtggccc ttgtcattgc gggtgatccg cgcgccgttc agatgcgcct cgcgttccag
      601 gaggtcgatc cacggctgcg gaaaccaatg ggcgtgcaga tcgaagatcg gcatggcgtt
      661 gccctcagag tcccatcagc cgccgcgcgt tgccgccgag gatgagccgc cgctcctctt
      721 cggagagcgc gggcacttcg cgcacgaatt ccaccggccg ctcgcagctc atgtcctggt
      781 tgtagtcgct gccgaggacg atacggtccg caccgacgag ctcgatcagg aatcgcatga
      841 ttcgcggatg atggcttacg gtgtcgtaat ggaagcggcg cagatacgcg ctcgccggcc
      901 gcttcatgtg cgcaagctcc tttctcaccg ccacgccgcg gtcgaagcgg ccgatcaacc
      961 acgggaacgt gccgcctgcc tgcggcagca tcacatcgag gcgcggaaac gcgtccatca
     1021 ccccgccgaa cacgagcgaa gccgccgcga gaccgttctc ataggtgttg ccgcagagat
     1081 tgcgcatgaa gtgcgccgcg atgcgatcgc ccccgcaggg atagagcggg tgaagaaaga
     1141 tcggcagccc gagcgcttcg cagcgttcgt acaccggcca gaagcgctcg tcgtcgagat
     1201 tcgatccgtt gatgtgggtt gcgagataca ccacacgcat gcccggcagc ttcgccgcac
     1261 gctcgagttc gccgacggcc agttccggcg cctgcatcgg caactggatg gtgccgatga
     1321 agcgcgtagg gtgcgcctgg tgcacggcgg cgctcgcatc gttccacgtc tgcgcgagct
     1381 ccagtccgaa gccgggcggc gcccaataca ccatcggatt ggtgagcgag agcacgaagg
     1441 tcgcaatgcc acggcggtcc atctcggcga ggatcagctc cggctcgacc atgagcggcg
     1501 gcatcgccga gacgggttgc acgcccggca gcgagaaaac gggaacgcct cgtgcattgc
     1561 gcaccatgcg tgcgccgtgt tccgcggctt tgcgctcgag caacgcgatg aatgccctcg
     1621 ggtaccagtg cgcgtgcgtg tcgatgacgg ccacgccgcc aggatgcctt gcggcgccct
     1681 ccccggattc gttcattgcg ccttcacggc ggcggcagcc ccatttcccg cgatgcggcc
     1741 gaagacgagg ccggatacga gcccggtccc cgccgggtaa ttgaagtaga aaatgccgcc
     1801 gaccatctcg cccgcggcat agagcccggg aatgggcgcg tatccgagat cgagcacctg
     1861 ccccgtttcg tgattgatgc gcagcccgcc gaaggtgaag gtgatgccgc aggtgacccc
     1921 ataggcctcg aacggcgggg tgtcgagcgg gttcgcccaa ttgcttttcg gcggatcgat
     1981 gccgacggtg cagcgcccgt cccgtatcgc gtggttgaac ggcacgtcgc ttcgcaccgc
     2041 ggtgttgtat tcacggatgg tcttcagaaa ctgccgggca tcgacgcctt ccatcttcgc
     2101 tgccagctcc tcgagcgtgt tcgcggtaaa ccgggtgaca ttccggccgc tgtactcctg
     2161 cttgaggaga tgcttcacct tggcatcgaa gagctggtag gcgtactgtc ccgtctgctt
     2221 gagcacctcg gcgccgtact tcgcgtaggt gtagttgtag aaatcggcgc cctcgtccac
     2281 gaagcgcttg ccctcggcat tgatcatgac cgagtacgga tagctgtgac ggtagggatc
     2341 gtgggagcgc tccacgactc cgaattccgg cgcgtgccgt tcccacgaaa ccgcgtggcg
     2401 gcccgaccag ttgccgtacg ggcaggcgcc gatctcgagc gccatccgca agccttcccc
     2461 catgttgtgc cgcgtgcccc gcaccttcgc cagctcccac ccggcgccga gatagcgcgt
     2521 gcgccattcg ggattcgcct cgaatccgcc gcacgcgagc accaccgccc gcgcacggaa
     2581 ctcgaccggc tcgccgcgct gcttcgcgcg cacccccagt acgcgcacgc cgtcatagac
     2641 gagcgaggtc gcacgcgtct cgtagaaaat ctcgatgccc ttcttcaccg cagtccgggt
     2701 gagttcctcc accagcgcgg cgccgccgcc gttcacggtg atgggaaagc ggccgaagaa
     2761 tttgcgcttg ccgttgacga tcgccgactg cgaaccgtaa ttcggaatga acttcgcgcc
     2821 ctggctgcgg agccacacca tcccttcgaa gcttttcgtg atgagcgtct cgctcaaatc
     2881 gggatcggtg cgg
//
LOCUS       DASURG010000254         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1065391, whole genome shotgun sequence.
ACCESSION   DASURG010000254 DASURG010000000
VERSION     DASURG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065391"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFY80_04370"
     CDS             complement(<1..329)
                     /locus_tag="VFY80_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6007172.1"
                     /translation="MIEFKNVSKWYGDFQVLKNCSTRVGKGEVVVVCGPSGSGKSTLI
                     KCVNGLEPFQQGEIVVDGKSVGARDTNLPQLRSKIGMVFQHFELFPHMTVTDNLNLAQ
                     VKVLGRSR"
     gene            complement(339..1031)
                     /locus_tag="VFY80_04375"
     CDS             complement(339..1031)
                     /locus_tag="VFY80_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6007173.1"
                     /translation="MFAGYDFDVIQRSLPYLFREGMTFTLTLTFLAMSGGLIFGTLLA
                     LMRLSSFAPLSALAGGYVNLMRSVPLVLVIFWFYFLVPYIAAWVVGSPRPIPIGTFWS
                     ALITFTMFEAAYYCEIMRAGIQSIPRGQVMAGYALGLTYGQTMVRIVLPQAFRNMLPV
                     LLTQTIILFQDTSLVYVISATDFLGAAVKIANRDYRLVEMYTFVALVYFILSFTASYF
                     VRRLQLRVAVIR"
     gene            complement(1035..1796)
                     /locus_tag="VFY80_04380"
     CDS             complement(1035..1796)
                     /locus_tag="VFY80_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6007174.1"
                     /translation="MKYDWQWSVYLQPAADGSGTWLMYLIVGLGWTLATALAAWVLAL
                     VIGAVVGTLRTTPMKWVVRAGNLYVEIFRNVPLLVQMFLWYFVLPELLPATLGDAMKQ
                     MAPPWGSYVPAVLCLGIYTSVRVAEQLRAGIQSLPRGQSMAGTALGLTLPQTYRYVLL
                     PMVFRIVLPPLTSEFLNIIKNSSLALTIGLLELTGRARSMQEFTFKVFEAFAAATVIY
                     LLTNLVVVLLMRRLERRVRVPGFIGSAPQMPQAGH"
     gene            complement(1863..2777)
                     /locus_tag="VFY80_04385"
     CDS             complement(1863..2777)
                     /locus_tag="VFY80_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6007175.1"
                     /translation="MKRLALPLSILFVAAGLAANATAAELTGTLKKIKDSGSITIGHR
                     DASVPFSYYDDRQRPIGYAVDLCLKVVDAIKAELKMPKLDVKYQLVTSANRIPLMANG
                     TIDLECGSTTNNLARQKEVAFTITHFLTATRLVSKKSANLKSIDDLKGKTVTSTAGTT
                     NIKQITDLNTSKNMGMNIIPANGHPEAFQMVETGRAVAFAMDDILLYSLVSQSRNPSD
                     YVISDFALSLPEPYGMMMRKDDPAFKKVVDAAMTRTYKSGEINKIYDKWFQSPIPPKN
                     INLKVPMSPLFKNVVAKPTDSGDPTAYK"
     gene            2957..>4126
                     /locus_tag="VFY80_04390"
     CDS             2957..>4126
                     /locus_tag="VFY80_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX6007176.1"
                     /translation="MPHPATRLEHDLLGERRVPAAAYYGVHTLRALENFPITRTPISI
                     YPELVSALACVKQAAALANNELTLLDDGKTRAVVRACEEIREGALLEEFVVDVIQGGA
                     GTSTNMNANEVIANRALEIMGRAKGEYQYLHPLEHVNMSQSTNDVYPTAIKVALHFGI
                     QRLTAAMAELRAAFLLKAVEFADVLKVGRTQLQDAVPMTLGQEFSTYAVMLEEDEQRL
                     REAMLLIHEINLGATAIGTGITAHPDYAAIVRRRLADITGIQLVTASNLVEATQDAGA
                     FVQVSGVLKRVAVKLSKTCNDLRLLSSGPRAGLNEINLPAVQAGSSIMPGKVNPVIPE
                     VVNQIAFEVIGNDVTVSFAAEAGQLQLNAFEPVIAHSLFKSLMHLRNGCEALRERC"
BASE COUNT          817 a         1260 c         1317 g          732 t
ORIGIN      
        1 cggctgcggc cgagcacttt cacctgcgcg agattgagat tgtcggtgac agtcatgtgc
       61 ggaaaaagct cgaagtgctg gaacaccatg ccgatcttcg agcgaagctg cggcaagttc
      121 gtgtctctcg ccccgacgga cttgccgtcc acgacgattt cgccctgctg gaagggctcc
      181 aggccgttca cgcacttgat gagcgtggac ttgcccgagc cggagggccc gcagaccacg
      241 accacttccc ccttgccgac ccgcgtgctg cagttcttca gcacctggaa gtcgccgtac
      301 cacttgctca cgttcttgaa ttcgatcatc gccgttctct aacggatcac cgcgacccgc
      361 agctgcagtc ggcggacgaa atatgacgcg gtgaagctca ggatgaaata gacgagcgcg
      421 acgaaagtgt acatctccac gagccggtaa tcgcggttcg cgatcttgac cgccgcgccc
      481 aggaagtcgg tcgccgaaat cacgtacacg agtgatgtat cctggaacag aatgatggtc
      541 tgcgtaagca gcaccggcag catgttccgg aacgcctgcg gcagcacgat cctcaccatc
      601 gtctggccgt aggtgagacc gagcgcgtag cccgccatta cctggccgcg cgggatgctc
      661 tggatgccgg cgcgcatgat ctcgcaatag tacgccgctt cgaacatcgt gaacgtgatg
      721 agcgccgacc agaaggttcc gatcgggatc ggccggggtg accccaccac ccatgccgcg
      781 atgtagggca cgaggaagta gaaccagaaa atgacaagca ccaggggcac ggagcgcatc
      841 aagttcacgt agccgccggc gagcgcggag agcggcgcaa agctcgagag acgcatcaac
      901 gcaagcagcg ttccgaagat gagcccgcca ctcattgcga gaaacgtgag cgtgagcgta
      961 aaggtcatcc cctcccggaa aaggtaggga agcgagcgct ggatgacgtc gaagtcgtag
     1021 ccggcaaaca tctctcagtg ccctgcctgc ggcatctgcg gggcggaccc gatgaagccg
     1081 ggtacgcgca cccgccgctc gagcctccgc atgaggagca ccacgacgag atttgtcagg
     1141 aggtagatca ccgtcgcggc cgcgaacgct tcgaacacct tgaacgtgaa ttcctgcatc
     1201 gagcgcgcgc gtccggtcag ttcgaggagc ccgatcgtga gcgcaagcga ggaattcttg
     1261 atgatgttga gaaactccga tgtgagcggc gggagcacga tgcggaacac catcggcaac
     1321 agcacgtagc gatacgtctg cggcagcgtg agcccgagcg cggtgccggc cattgactga
     1381 ccgcgcggca gcgactggat cccggcacgc aactgctcgg cgacgcgcac cgaggtgtag
     1441 atcccgaggc acagcaccgc cggaacatac gagccccacg gcggcgccat ctgcttcatg
     1501 gcgtccccga gcgtcgcggg caggagttcc ggcagcacga agtaccaaag gaacatctgc
     1561 acgagcagcg gcacgttgcg gaagatctcg acatagaggt tgcccgcgcg caccacccat
     1621 ttcatcggtg tcgtgcgcag cgtcccgacc accgcgccga tcacgagcgc gagcacccac
     1681 gcggcgagcg cggtcgcgag cgtccagccg aggcccacga tgaggtacat gagccaggtg
     1741 ccgctgccgt cggccgcggg ctgcaggtag acgctccact gccagtcgta tttcacgagt
     1801 ccaatgccgc cgacacaaac aaaatggggg accgttcggt cccccgcgcg tcgggctgac
     1861 tcttacttgt aggccgtcgg gtcgccggag tcggtcggct tcgcgaccac gttcttgaac
     1921 agcggcgaca tcggcacttt cagattgatg ttcttcggcg ggatcgggct ctggaaccac
     1981 ttgtcgtaga tcttgttgat ttcgccgctc ttgtaagtcc tggtcattgc agcatccacg
     2041 accttcttga acgccggatc gtccttgcgc atcatcatgc cgtagggctc cggcagcgac
     2101 agtgcgaaat ccgagatcac gtagtcagac ggattgcgcg actgcgagac caggctgtag
     2161 agcaggatgt cgtccatcgc gaaggcgacc gcgcgacccg tctccaccat ctggaaggct
     2221 tcggggtgcc cgttcgcagg aatgatgttc atgcccatgt tcttggacgt gttgaggtcg
     2281 gtgatctgct tgatgttggt cgtgccggcg gtcgaggtga cggtcttgcc tttgagatcg
     2341 tcgatcgact tgaggttggc cgatttcttc gagacgagtc gcgtcgcggt caggaagtgc
     2401 gtgatggtga aggccacttc cttctgccgc gcgagattgt tggtggtcga tccgcactcg
     2461 aggtcgatcg tgccgttcgc catgagcggg atgcggttgg cggaggtcac gagctgatac
     2521 ttgacgtcga gcttcggcat tttgagctcg gccttgatcg catccaccac cttgaggcag
     2581 aggtccaccg cgtagccgat cggccgctgc ctgtcgtcgt aataggaaaa aggcaccgaa
     2641 gcgtcacgat ggccgatcgt gatcgagccg ctgtccttga tcttcttgag cgtgccggtc
     2701 agttcggccg ccgtggcatt cgcggccaag cctgcggcaa cgaacaggat cgacaagggc
     2761 aatgccagcc gtttcatgag tatctcctct cggtgcgatt cgcggacgga atgggatttg
     2821 ggggcgtgag ccgcttgcca atctagccga acggcatacg tttgtccagc aacgaatccg
     2881 agaagaaaca atcggctaca gacgatttgg cgctatagtt gaggcccgaa tccgaagcct
     2941 ttccgggaga tatgcgatgc cgcatcccgc cacccgtctc gaacacgatc tgctcgggga
     3001 gcggcgcgtg ccggccgccg catattacgg tgtccatacg ctgcgcgcgc tcgagaactt
     3061 tccgatcacg cgcacgccga tctcgatcta tcccgaactg gtgagcgcgc tcgcctgcgt
     3121 gaagcaggcg gcggcgctcg cgaacaacga gctcacactg ctcgacgacg ggaagactcg
     3181 cgccgtcgtg cgcgcctgcg aggagatccg ggagggggcg ctactggagg aattcgtggt
     3241 ggacgtgatt cagggcggcg ccggcacctc gaccaacatg aacgcaaacg aagtcatcgc
     3301 gaaccgcgcg ctcgaaatca tggggcgcgc caagggtgag taccagtacc tgcacccgct
     3361 cgagcacgtc aacatgagcc agagcaccaa cgacgtgtac ccgacggcga tcaaggtcgc
     3421 gctgcacttc ggcatccagc ggctcaccgc agcgatggcg gaactgcgcg cggcattcct
     3481 gctgaaggcg gtcgaatttg ccgatgtgct gaaggtggga cgcacgcaac tccaggacgc
     3541 ggtgccgatg acgctgggcc aggagttttc gacctacgcc gtcatgctcg aggaagacga
     3601 gcagcgactg agggaggcga tgctcctgat ccacgagatc aacctgggag cgaccgcgat
     3661 cgggaccggc atcaccgcgc atccggatta cgctgccatc gttcgccggc ggctcgcgga
     3721 catcacgggc atccagctcg tgaccgcctc caatttggtg gaggcgacgc aggatgccgg
     3781 cgcgtttgtc caggtttccg gcgtattgaa gcgcgtcgcc gtcaagctct cgaaaacctg
     3841 caacgacctg cggctgctct cttcgggccc gcgggcggga ctgaacgaga tcaatctccc
     3901 ggcggtgcag gcgggctcga gcatcatgcc gggtaaggtg aaccccgtca ttcccgaagt
     3961 ggtgaaccag atcgcgttcg aagtcatagg gaacgacgtg acggtgagct tcgccgcgga
     4021 ggccggccag ctccagctca atgccttcga gccggtcatt gcgcacagcc ttttcaagag
     4081 cctcatgcac ctgcggaacg gctgcgaggc gctgcgcgag cgttgc
//
LOCUS       DASURG010000255         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_147087, whole genome shotgun sequence.
ACCESSION   DASURG010000255 DASURG010000000
VERSION     DASURG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147087"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFY80_04395"
     CDS             <1..1170
                     /locus_tag="VFY80_04395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6007177.1"
                     /translation="RRPGAAAPMTAVRELRVEPIRATARPATSVRELRAEDIPQIAAL
                     HGRVFGTTPSRTPQRLAAYFEEILFRNPWRDASLPSLVAAKNGHITGFLGVVPRPMKL
                     HDRAIRVAVCTQFMVDPEHRGELGAVQLLKTFFSGPQDLSLADGANEHARRMWLALGG
                     AAPLLYSLHWLRPLRPARYVLSLIERRATTLRTMAYAGRPVAALADAAIARAPLSRLH
                     EDRNDAVEETLQPSTMCAYLAEVMSGTVLQPRYDVSALGWLLDQAAHMTRHGTLRARA
                     VRDTERRLLGWFLHYVRAGGVSEVVQVAARAGAFNRVLQRLVLDAWRHGATAVRGRLD
                     PRYAQELSDQHCWLRRDCNWTLVHTRDDDISSAIHQGKIFLTRLEGEWWMRFLEE"
     gene            1192..2349
                     /locus_tag="VFY80_04400"
     CDS             1192..2349
                     /locus_tag="VFY80_04400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6007178.1"
                     /translation="MKICFVGLDNLPVLAPEFERLPIGGESVQQTLLARAMMRRGCEV
                     SMVVADRGQPDGAAWSGIRAFNAYRPEAGFPVLRFLHPRWTRMWSALARADADLYYTS
                     CAGMHVGLMALFCRRYRRRFVFRAASDPDCDRARLPLLVRHARDRWLYTYGLRRADAI
                     LVQSAVQADALARSYGLPSRIAGMLVDTPPFSTTRDIDVLWVGNIKRVKRPDRILEIA
                     EKLPGARIHVAGGETPGEEPLFEAFSRGVARSPSLVYHGRLPYAAAAALYGRSRLLVN
                     TSDVEGFPNAYLQSWASGVPVVTLIDPDGIIAIEGLGVVARSDSELSAAIGALLGDAA
                     SWNAASARCRAYMAREYSEERITTDYLDTFRTVMRIPSVTGRGLAPSRHHV"
     gene            2342..>2720
                     /locus_tag="VFY80_04405"
     CDS             2342..>2720
                     /locus_tag="VFY80_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HEX6007179.1"
                     /translation="MSETPFIITVDTEGDNMWARPREITTRNAAFLPRFQALCERYRF
                     KPVYLTNHEMAMSDLFVEFGRDVIERDAGEIGMHLHAWNSPPLYSLTGDDYRYQPYLI
                     QYPAAVMKEKIRTLTALLEDRFGR"
BASE COUNT          433 a          926 c          873 g          488 t
ORIGIN      
        1 cggcgcccgg gtgcggcagc tccaatgaca gccgtccgcg aactccgggt cgaaccgatt
       61 cgtgcgacag cgcgtcccgc gacgtccgtg cgcgagctcc gggccgagga cataccgcag
      121 atcgccgcat tgcatgggcg cgtcttcggc actacgccgt cgcgcacgcc gcaaagactc
      181 gccgcctatt tcgaggagat cctgttccgc aatccgtggc gcgatgcttc cctgccttcg
      241 ctcgttgccg ccaagaacgg tcacataacg ggtttccttg gcgtcgtccc gcggccgatg
      301 aagctccatg accgggccat ccgcgtcgcg gtgtgcaccc agttcatggt tgaccctgaa
      361 catcgcggcg agctgggagc cgttcagctt ctgaagactt ttttctcggg cccccaggac
      421 ctttcgctcg ctgacggagc caacgagcac gcgcggcgca tgtggctcgc tctcgggggc
      481 gcggcgcccc tgctctacag cctgcactgg ctgcgcccgt tgcgtccggc acgttatgta
      541 ctatcgctga tcgagcgacg cgcgaccact ctgcgcacga tggcgtacgc gggacgcccg
      601 gtggccgcgc tcgccgatgc ggcgatcgcc cgcgcgcctc tgagccgcct gcacgaggac
      661 cgcaacgacg cggtggagga aacgttgcag ccgagcacca tgtgcgcgta tctggccgaa
      721 gtcatgtcgg gcacggtctt gcagccgcga tacgacgtgt ccgccctggg atggctgctc
      781 gatcaggccg cgcacatgac acggcatgga acgctgcgcg cgcgcgccgt gcgggatacc
      841 gagcgccggc tgctgggctg gttccttcat tatgtgcggg cgggcggcgt gagcgaggtt
      901 gtgcaggtcg ccgcgcgtgc cggtgccttc aaccgcgtcc tgcagcgcct cgtcctcgat
      961 gcgtggcgtc atggggcgac cgcagtgcgc ggccgcctcg atccgcgcta tgcgcaggag
     1021 ctttcggacc agcactgctg gctgcgtcgc gactgcaact ggacactggt gcatacccgc
     1081 gacgacgata tttcttccgc catccatcag ggaaagattt tcctcacccg cctcgagggc
     1141 gaatggtgga tgcgtttcct cgaggaatga caaatccgcc gtgaccgagc gatgaagatc
     1201 tgttttgtcg gcctggacaa cctccccgtg ctggcgccgg aattcgagcg acttcccatc
     1261 ggcggagagt ccgttcaaca aacgctcctt gcccgcgcaa tgatgcgacg cggctgcgag
     1321 gtatccatgg tggttgcaga ccgcgggcag ccggacggcg cggcgtggag cggaatcagg
     1381 gctttcaacg cgtaccggcc cgaagcgggc tttccggtgc tgcgtttttt gcacccgcgc
     1441 tggacccgca tgtggtccgc gctggcgcgc gccgatgccg atctctatta caccagttgt
     1501 gccggcatgc acgtcggcct catggcgctc ttttgccgcc gttaccgccg gcgcttcgta
     1561 tttcgcgcag cgagcgaccc ggactgcgac cgcgcgcggc tgccgcttct cgtccgccat
     1621 gcacgcgatc gctggctcta cacctatgga ctgcgcaggg ccgatgccat tctcgtgcaa
     1681 agcgcggtgc aggccgacgc gctcgcgcgg agctatgggc tcccttcccg catcgccggc
     1741 atgctcgtcg atacgccgcc cttctccacg acccgcgata tcgacgtgct gtgggtcggg
     1801 aacatcaagc gcgtgaagcg gcccgaccgt atcctcgaga tcgcggaaaa attgcccggc
     1861 gcgcggattc atgtcgccgg cggcgaaaca ccgggagaag aaccgctgtt cgaagcgttt
     1921 tcgcgcggcg tggcgcgctc gcccagcctc gtctatcacg gtcgcctgcc gtacgccgcc
     1981 gcggccgcgc tgtatggccg cagccggcta ttggtgaaca cctccgacgt cgaaggtttc
     2041 cccaacgcct acctgcagtc ctgggcgagc ggagttccag tcgtcacgct gatcgacccc
     2101 gatggcatca tcgcgataga agggctcggg gtcgttgcgc gctcggacag cgaattgagc
     2161 gccgcgatcg gagcgctgct cggcgacgcg gcctcctgga acgcggccag cgcacgctgc
     2221 cgcgcctaca tggcccgcga gtacagcgag gagcggataa cgactgatta tctcgacact
     2281 ttccgcaccg tgatgcggat tccctctgtc acgggacgcg ggctcgcgcc cagccgccat
     2341 catgtctgaa accccgttca tcatcaccgt cgacaccgag ggcgacaaca tgtgggcccg
     2401 gccgcgggag attaccacgc gcaacgccgc gtttttgccc cgcttccagg cgctgtgcga
     2461 gcgttaccgc ttcaagccgg tgtacctgac gaaccacgaa atggcgatgt cggatctctt
     2521 cgtcgaattc gggcgcgatg tgatcgagcg cgacgcggga gaaatcggca tgcacctgca
     2581 cgcgtggaat tcgccgccgc tgtactccct caccggagac gactaccgct accagcccta
     2641 cctcatccag tatccggccg cggtgatgaa ggaaaagatc agaacgctca cggcgctgct
     2701 cgaagaccgg ttcgggaggc
//
LOCUS       DASURG010000256         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_250328, whole genome shotgun sequence.
ACCESSION   DASURG010000256 DASURG010000000
VERSION     DASURG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250328"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VFY80_04410"
     CDS             complement(<1..113)
                     /locus_tag="VFY80_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6007180.1"
                     /translation="MADTIEVKVPDIGDFTDIPVIDVLVKPGDRVDKEDSL"
     gene            complement(183..1439)
                     /locus_tag="VFY80_04415"
     CDS             complement(183..1439)
                     /locus_tag="VFY80_04415"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX6007181.1"
                     /translation="MDVQTYMVGVGRQARAAARHIARADTGMKNRALAIMADAIGRAT
                     PALIQANARDLEAAQARKLEPALIDRLTLGKQTIEAMADGLRQITALPDPVGEIIGLK
                     YRPSGIQVGQMRVPLGVIGIIYESRPNVTADAAGLCIKSGNAAILRGGSEAIHSNQAI
                     AACVHEGLRAVGLPESAVQVIETTDRAAVGELITMQDYVDVIVPRGGKSLIERLMRES
                     RIPMIKHLHGVCHVYIDDRADLDKAIRVADNAKTQRLGTCNTMETLLVARGIAQKVLP
                     PLCRTFLEKGIELRGDETARHIVPQMTAATEGDWYTEYLDAILSVRVVDGLDQAIDHI
                     ATYGSQHTDAIVTEDWTRARRFLAEVDSSSVMVNASTRFADGYEYGLGAEIGISTDKL
                     HARGPVGLEGLTSLKYVVLGDGHIRT"
     gene            complement(1467..2549)
                     /gene="holA"
                     /locus_tag="VFY80_04420"
     CDS             complement(1467..2549)
                     /gene="holA"
                     /locus_tag="VFY80_04420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX6007182.1"
                     /translation="MKLTTEQLPQHLARELKPLYTIFGDEPLLALEAGDRVRAAARDA
                     GYTERVVLTADAGFKWRELALAASSQSLFSARKLLELRIPTGKPGTDGAGALQSYCAS
                     LPPDTVTLVSLPSLEWRAQKAAWFEALTAAGILVEAKVVPRKALPQWIAGRLRAQDQH
                     ADPETLDFIANRVEGNLMAAYQEVRKLALLFPPGAISCDQAHDAVLDVARFDVFNLGE
                     ALLEGEPLRLARMLDGLKGEGSAPPLVLWAMCEEIRAIGKLISGVAAGKPLSMLWREA
                     RIWAPAHQHLMQQNLSRFTLVQIAEALRHAAAIDRTIKGLAKGDVWDELLHLALRFAR
                     GGKAPANRGRMGSRGAEAARSQAGLF"
     gene            complement(2758..>3181)
                     /gene="lptE"
                     /locus_tag="VFY80_04425"
     CDS             complement(2758..>3181)
                     /gene="lptE"
                     /locus_tag="VFY80_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX6007183.1"
                     /translation="ATLPFDSIYVQGSSTSPFTIQLKRSISSGTKVRLMENPKEAEVV
                     LQILSERQEKEILALSGGGRVREYYLRYNVTYRLTSSKGIEYIPPTQISLRRELTYSD
                     AEALAKESEEMLLFRDMQSDAARQLLFRLQVVKLDAKA"
BASE COUNT          556 a         1020 c         1036 g          569 t
ORIGIN      
        1 atgagggaat cctccttgtc cacacggtcg ccgggcttga ccagcacgtc gatgacaggg
       61 atgtccgtga aatcgccgat atcgggaacc ttgacttcga tcgtgtctgc catgagagag
      121 ccttgtcagc ggaacggatc aacaaaacgg ggccggacgg caccaccgaa cgccgccgaa
      181 cactaagtgc ggatgtggcc gtccccgagc accacgtact tgagggaagt gagtccttcg
      241 aggccgaccg ggccgcgcgc atgaagcttg tcggtcgaga tgccgatttc cgcgccgaga
      301 ccgtactcgt agccgtcggc gaagcgggtg gatgcgttca ccatgaccga actcgaatcg
      361 acctccgcga ggaagcggcg cgcgcgcgtc caatcctcgg tcacgatggc gtcggtgtgc
      421 tgcgagccgt aagtcgcgat gtggtcgatc gcctgatcca ggccgtccac gacccgcacc
      481 gacagaatcg cgtcgaggta ctcggtgtac cagtcgccct cggtcgccgc tgtcatctgc
      541 ggcacgatgt ggcgcgccgt ctcgtcgccc cgaagctcga tgcctttctc gagaaatgtc
      601 cggcaaagcg gcggcagaac cttctgcgcg atgccgcggg cgacgagcag cgtctccatc
      661 gtgttgcacg ttccaaggcg ctgcgtcttg gcgttgtccg caacgcggat cgccttgtca
      721 aggtcggcgc gatcgtcgat gtagacgtgg cacacgccgt ggagatgctt gatcatcggg
      781 atgcgcgact cgcgcatcag gcgctcgatc aggctcttgc ccccgcgcgg cacgatcacg
      841 tcgacatagt cctgcatggt gatgagttcg cccacggcgg cgcggtccgt ggtctcgatc
      901 acctgcaccg ccgactcggg caggccgacc gctctcagcc cttcgtgcac gcatgcggcg
      961 atggcttgat tggaatggat cgcttccgag ccgccgcgca ggatcgcggc attgccggac
     1021 ttgatgcaaa gccccgcggc atccgcggtg acgttggggc gggactcgta aatgatgccg
     1081 atcacgccga gcggcacgcg catctgtccc acctggatcc cggaggggcg gtacttgagg
     1141 ccaatgattt caccgacggg atccgggagc gctgtgatct ggcgcaggcc gtcggccatc
     1201 gcctcgatcg tctgcttgcc gagcgtcagc cgatcgatga gcgcgggctc gagctttcgc
     1261 gcctgtgccg cttcgagatc gcgtgcgttc gcctggatga gtgcaggcgt cgcacgcccg
     1321 atcgcatcgg ccatgatggc gagcgcgcgg ttcttcatgc cggtgtccgc ccgcgcgatg
     1381 tggcgtgcgg cagcgcgcgc ctggcggccc acgccgacca tgtaagtctg aacgtccacg
     1441 tatgacctcg taggcgaccg agggtcctag aacaggcccg cctgactgcg tgcggcctcg
     1501 gctccgcgtg agcccattct accccgattc gcaggcgcct tgccgccgcg cgcgaagcgc
     1561 agcgcgagat gcagcagctc gtcccacacg tcgcccttcg cgaggccctt gatcgtgcgg
     1621 tcgatggctg ccgcatgccg cagtgcttcg gcgatctgga cgagcgtgaa gcggctcaga
     1681 ttctgctgca tgagatgctg atgcgcaggc gcccagatgc gcgcttcgcg ccacagcatc
     1741 gaaagcggct tgcccgccgc gacaccggag atgagttttc cgatggcgcg gatttcctcg
     1801 cacatcgccc acaatacgag gggcggcgcg ctgccttcgc ccttgaggcc gtccagcatg
     1861 cgcgcgagcc gtagcggttc gccctccagc agcgcttcgc cgaggttgaa aacgtcgaag
     1921 cgcgccacgt cgagcactgc atcgtgcgcc tgatcgcacg agatcgcgcc cggtggaaaa
     1981 agcagcgcga gcttgcgcac ttcctggtat gccgccatga gattgccctc gacgcggttg
     2041 gcgatgaaat cgagcgtctc ggggtccgcg tgctggtcct gcgcccggag ccgacccgcg
     2101 atccattgcg gcagcgcctt gcgcggaacg actttggctt cgaccaggat gccggcggcc
     2161 gtgagcgctt cgaaccaggc ggctttctga gcgcgccact caaggctcgg caacgatacg
     2221 agggtgacgg tatcgggggg cagggatgca caatacgatt ggagcgcgcc cgccccgtcg
     2281 gtccccggtt tgccggtcgg tatgcgtagc tcgaggagct tgcgcgcgga aaacagcgac
     2341 tgggaactgg cggcgagagc gagttcccgc cacttgaatc ccgcatcggc agtgagcacc
     2401 acgcgctcgg tgtagcccgc gtcacgcgcc gccgcgcgga cgcgatcgcc tgcttcgagc
     2461 gcaagcaggg gttcgtcgcc aaaaatcgta taaagcggct tcaactcgcg cgcgaggtgc
     2521 tgcggcaact gctcagtcgt taatttcatt taatcggccg cggaaagcgc tagcatcgaa
     2581 aaatcttagc cgcgaacacg ccactgcgca aagcatttcg cgcgcgggca agcacaaatc
     2641 gggcggggac gagattggac gaagctaatg tgccatcacg cgagcgaacg tcgtcattcc
     2701 ggggacagtc agcatgcccc ggcatgtatc accggcatga ccagtctcgc atcaacacta
     2761 cgccttggca tcgagcttga cgacctgcag ccggaaaagc agctggcgcg cggcgtccga
     2821 ctgcatgtcg cggaaaagca gcatttcctc cgattccttg gcgagcgctt cggcgtcgct
     2881 gtacgtgagc tcgcggcgga gcgaaatctg cgtcggcgga atgtattcga tgcccttgct
     2941 gctggtcagg cgataggtga cgttataccg cagataatat tcgcgcacgc ggcccccgcc
     3001 cgaaagcgcg agaatctcct tctcctggcg ctcgctcaga atctgcagca cgacctccgc
     3061 ttccttcgga ttttccatga ggcgcacctt ggtgcccgag gagatcgagc gcttgagctg
     3121 tatggtgaag ggcgaagtcg agcttccctg cacatagatg gagtcgaacg gcagggtcgc
     3181 c
//
LOCUS       DASURG010000257         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1373505, whole genome shotgun sequence.
ACCESSION   DASURG010000257 DASURG010000000
VERSION     DASURG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373505"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>2180
                     /gene="purL"
                     /locus_tag="VFY80_04430"
                     /gene_synonym="purI"
     CDS             66..>2180
                     /gene="purL"
                     /locus_tag="VFY80_04430"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX6007184.1"
                     /translation="MSQLIRLRGRSALSAFRLQKLLQKVAAAVSGINLDAEYWHFVAL
                     RAELSRSERERLERLLTYGPSATPARSEGTLCLVVPRIGTISPWSSKATDIAHHCGLG
                     QVERIERGIAYWIDRTLSDAEKTALAALIHDRMTETVLDSFDHVPRLFQHVAPQPLKT
                     VDLMRDGQAALERANRDMGLALSPDEIAYLGEHFSRAGRNPTDVELMMFAQANSEHCR
                     HKIFNADWVIDGQAQEQSLFGMIRTTHEKHPRGTVVAYADNAAVMEGATVARFYPRAD
                     GTYRFSDETTHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLAGF
                     SVSNLRIPGGEQPWEHDHGRPGRIASAFQIMVDGPVGAASFNNEFGRPNLCGYFRTYE
                     QRVGDEIRGYHKPIMIAGGLGNIAATQAHKQPLEAGALVIQLGGPGMLIGLGGGAASS
                     MDTGANLEELDFDSVQRGNAEIQRRAQEVIDRCVALDAENPILSIHDVGAGGLSNALP
                     EMVHAAGRGGHFELRRIPSEEPGMSPLQIWCNEAQERYVLAIAPQALERFAAICERER
                     CPFAVVGEATGDGQLKVTDAQFDNRPVDMPLAVLLGKPPRMTRDVARVRRKFPAFDTR
                     HIDLADAAYRVLRLPTVADKTFLISIGDRTVGGLTARDQMVGPWQVPVADVAVTLMGY
                     RTYRGEALAMGERTPLAVIDPEA"
BASE COUNT          379 a          721 c          725 g          355 t
ORIGIN      
        1 ggctagttta gcggaaagcg ggtggagatt tggtaaaatg cctttttata gagtcgcctc
       61 agctcatgtc gcaactcatc aggctgcgtg gacgcagcgc cctctccgct tttcgcctcc
      121 agaaactcct tcagaaagtt gcagccgccg tctccgggat aaacctggac gcggaatact
      181 ggcacttcgt tgcgctgcgc gccgagctgt cgcgcagcga gcgcgagcgc ctggaacgac
      241 tcctcactta cggccccagc gccacgccgg cacgcagcga aggcacgctt tgtctcgtcg
      301 tcccgcgcat cggaacgata tcgccctggt cttcgaaggc gaccgacatc gcgcaccatt
      361 gcgggctggg gcaggtcgag cgcatcgagc gcgggatcgc gtactggatc gatcgcacgc
      421 tttccgatgc ggaaaagacg gcgctcgcgg cgctgataca cgaccgcatg acggagaccg
      481 tgctcgattc cttcgatcac gtgccgcggc ttttccagca cgtcgcgccg caaccgctta
      541 aaacggtgga tctcatgcgc gacgggcagg cggcgctgga acgtgccaac cgcgacatgg
      601 ggctcgcgct ctcgcccgac gaaatcgcgt atctcggcga gcatttctcg cgcgccggac
      661 gcaacccgac cgacgtcgaa ttgatgatgt tcgcgcaggc gaactcggaa cactgccggc
      721 acaagatttt caacgccgac tgggtgatcg acgggcaagc gcaggagcag tcgctcttcg
      781 gcatgatccg cacgacgcac gagaagcatc cgcgcggcac ggtcgtcgcg tatgccgaca
      841 atgccgccgt aatggaaggc gcgactgtcg cccgtttcta tccccgcgcc gatggcacct
      901 accggttcag cgatgagacg acgcacatcc tgatgaaggt cgagacgcac aaccatccga
      961 ccgcgatttc gccgttcccg ggcgcggcca cgggctccgg tggagaaatc cgcgacgagg
     1021 gcgcgaccgg ccgcggcgcc aagcccaagg ccgggcttgc gggcttcagc gtgtcgaacc
     1081 tgcggatccc gggcggcgag cagccgtggg agcacgacca tggccggccc gggcgcatcg
     1141 cttcggcatt ccagatcatg gtcgatggtc ccgtgggggc ggcctcgttc aataacgagt
     1201 tcggccgccc gaacctctgc ggctatttcc gcacctacga gcagcgtgtc ggcgacgaga
     1261 tccggggcta ccacaagccc atcatgatcg ccggagggct cggcaacatc gcggcaaccc
     1321 aggcgcacaa gcaaccgctg gaagcgggtg cgctcgtcat tcagctcggt gggcccggca
     1381 tgctgatcgg cctcggcggc ggcgcggcct cgagcatgga caccggcgcc aatctcgagg
     1441 aactcgactt cgattccgtg cagcgcggca acgcggagat ccagcgccgc gcccaggagg
     1501 tgatcgaccg ctgcgtcgcg ctggacgctg agaatccgat cctctcgata cacgatgtcg
     1561 gcgccggcgg gctttcgaac gcgctgcccg agatggtgca tgccgccggt cgcggcggcc
     1621 atttcgaatt gcgccggatt ccgagcgagg agccgggcat gtcgccgctg cagatctggt
     1681 gcaacgaggc gcaggagcgc tacgtgctcg cgatcgcgcc gcaggcgctc gagcgctttg
     1741 ccgcgatctg cgagcgcgag cgctgcccgt ttgcggtagt cggcgaggcg acgggggatg
     1801 ggcagctcaa ggtaaccgac gcgcagttcg acaatcgtcc ggtggacatg ccgctcgccg
     1861 tgctgctcgg caagccgccg cgcatgacgc gcgacgtggc gcgcgtgcgg cgcaagtttc
     1921 cggcgttcga tacccgccat atcgatcttg ccgatgccgc atatcgcgtg ctgaggttgc
     1981 ccacagtcgc cgacaagaca ttcctcatca gcatcggcga ccgcaccgtc ggcggcctga
     2041 ccgcacgcga ccagatggtg gggccgtggc aggtgccggt ggcggacgtc gccgtgacgc
     2101 tcatgggata tcgcacttat cgcggtgagg cgctggccat gggcgagcgc acgccgctcg
     2161 ccgtgatcga tcccgaggca
//
LOCUS       DASURG010000258         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_147440, whole genome shotgun sequence.
ACCESSION   DASURG010000258 DASURG010000000
VERSION     DASURG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147440"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFY80_04435"
     CDS             <1..353
                     /locus_tag="VFY80_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6007185.1"
                     /translation="TYGLRVGEARLNEAQGVRLDYVELGNAKIELMEPSRPDSPVAKF
                     LERNPGGGIHHFCLTVENVDAAAQAMRQKSARVLDDGSAQKNVHGARIAFVHPKDFLG
                     ALVEIEESEPKEKK"
     gene            350..1327
                     /locus_tag="VFY80_04440"
     CDS             350..1327
                     /locus_tag="VFY80_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007186.1"
                     /translation="MSTRMLLTAAILCAMPAFAAQPGPQNYPTKPIRLLIPFAPGGAT
                     DIIARMIEPKMSRALGQQIVVDNRPGAAGNIAVELAAHAQADGYTLLVGNISTNSINP
                     ILFARQMKVNAVKDLTGVSKLVAIPNFILGSPKLPANTLKEAIEYAKSRPGQLNYQAP
                     LGSYSHLDMLAFTAAAGIRMVHLPSRGAGETLPALLRGDVHITESNVASNIGPVRAGQ
                     IKAFAVTSEKRLGELPNVPTMAEAGFPGIGSLNWNGIFAPARTPKPVLDRLHGVATAA
                     MKELEAEGVLAKRQTPISLSATPEDFNAYVMSELKRWEKIIRDNNVKID"
     gene            1348..2409
                     /locus_tag="VFY80_04445"
     CDS             1348..2409
                     /locus_tag="VFY80_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX6007187.1"
                     /translation="MSEAAGKRIAVAELEGFIARAFVAVGIVEKDARAIAELMTAADV
                     QGSEGHGVFRLPQYIRRIKGGAVNLKPSIRIAREASGMALVDGDNGMGHLVMRFATET
                     AIAKAKSAGVAWVGVRWSNHAGPASLYAAMPLAHDMIGLYLAVGNANHLPPWGGIDML
                     LSTNPIAVALPAGEEAPVVLDMATTVAAYGKVKTKAQRGETMPEGWMIDRQGKPLLDP
                     KRASEGFLLPIGGYKGYGLALVIGLLAGTLNGAAMGKEVVDFNADDTTPTNTGHAIVA
                     INVEAFQPVAEFKRAVDTLIRDLRNSRTLPGVDRVRIPGEGSHAARTERGRYGIPLPP
                     ALCASLDTLARELGIAPLP"
     gene            2606..3208
                     /locus_tag="VFY80_04450"
     CDS             2606..3208
                     /locus_tag="VFY80_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6007188.1"
                     /translation="MKTMARLAGLFLTFAACAAFAAESTPDALVKRTVDEVMSVLKEN
                     KDRRKLVELAEQKVLPNFDFTEMTRLAVGRAWREANDAQKKALENAFRTLLVNTYTTA
                     LMQAAKADQTVEVKPVRVQPGQKEVVVKTVVKEPGRPAIPIDYSMRRGDSQWKVYDVV
                     VENLSLVTNYRNTFSSEVAKGGIDGLIKSLEDKNRRIAAG"
     gene            3512..>3985
                     /locus_tag="VFY80_04455"
     CDS             3512..>3985
                     /locus_tag="VFY80_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007189.1"
                     /translation="MIRFLYPRSFPRLLAVGFTLIALPLIFALIGSAVTVDQIANRSQ
                     QAVYRAVLATQTGRRLAELLIAMERSARQMVILDDRRLLETYESNRKHFVATAREFSA
                     LPFDSEQMSALGEIMSGEQAIYKALSTPYATTEELQKAVGGFVGLTEHAGAITARS"
BASE COUNT          786 a         1210 c         1321 g          668 t
ORIGIN      
        1 agacgtacgg attgcgcgtg ggagaggcaa gattgaacga ggcgcagggc gtgcggctcg
       61 attatgtcga gctgggcaat gcgaaaatcg agttgatgga gccttcgcgt cccgactctc
      121 ccgttgcgaa attcctcgag cgtaatcccg gcggcggcat tcatcatttc tgcctcacgg
      181 tggagaatgt cgatgccgcg gcgcaggcga tgcggcagaa gagcgcgcgc gtgctggacg
      241 acggctcggc gcagaagaac gtgcacggcg cccggatcgc cttcgtccat ccgaaggatt
      301 ttctcggcgc gctcgtcgag atcgaggaaa gcgagcccaa ggagaaaaaa tgagcacgag
      361 aatgcttttg accgctgcaa tcctctgcgc gatgcccgcg ttcgctgccc aaccggggcc
      421 gcagaactat ccgacgaagc cgatccgcct gctgattccc tttgcgccgg gcggcgcgac
      481 cgacatcatc gcgcgcatga tcgagcccaa gatgagcaga gcgctcgggc agcagatcgt
      541 ggtggacaac cgtcccggcg cagcgggtaa tatcgcagtg gagctcgccg cgcacgccca
      601 ggcggacggt tatacgctgc tcgtcgggaa catctccacc aattccatca acccgatcct
      661 gttcgcgcgc cagatgaagg tgaacgccgt caaggatctg accggcgtgt ccaagctcgt
      721 tgcgattccc aacttcatcc tcggcagccc gaagttgcct gcgaacaccc tcaaggaagc
      781 gatcgaatac gcaaagtcgc gtcccggaca gctcaactat caggcgccgc tcggatcgta
      841 ttcgcatctc gatatgctcg cctttaccgc ggcggccgga atcaggatgg tgcacctgcc
      901 ttcgcgcggt gccggcgaga cgctgcccgc gctgctccgc ggagatgtcc acatcaccga
      961 gtcgaacgtc gcttccaaca tcgggccggt gcgtgccggc cagatcaagg cgttcgcggt
     1021 gacatccgag aagcgtcttg gcgaactgcc caacgtgccg accatggcgg aggcgggttt
     1081 tcccggcatc ggaagcctca actggaacgg catcttcgcg ccggcccgca cgccgaagcc
     1141 cgtactggac cggctccacg gtgttgcgac cgcagccatg aaggagctgg aagcggaagg
     1201 cgtgctggcg aagcgccaga cgccgatcag tctcagcgct actcccgaag acttcaacgc
     1261 ctacgtcatg tcggaattga agcgctggga gaagatcatc agggacaaca acgtgaagat
     1321 cgactgagtg cagggcggtg gacgaacatg agtgaagcgg cgggtaagcg gattgcggtt
     1381 gcggaactcg agggctttat tgcgcgcgcg ttcgtggcgg tcggcatcgt tgaaaaagac
     1441 gcacgcgcca ttgccgagtt gatgaccgcc gcggatgtcc agggctccga gggccatggg
     1501 gtcttccggc tgccgcagta catccggcgc atcaagggcg gcgcggtgaa cctgaaaccg
     1561 agcatccgca tcgcgcgtga agcatcgggc atggccctcg tcgacggcga caacggcatg
     1621 ggccacctcg tcatgcgctt tgccaccgaa accgcgatcg cgaaagcaaa gagcgcgggc
     1681 gttgcgtggg tcggggtgag atggagcaat cacgccggac ccgcgtcgct ctacgctgcg
     1741 atgccgcttg cgcatgacat gatcgggctc taccttgccg tgggcaatgc caatcatctg
     1801 ccgccgtggg gtgggatcga catgctgctt tccacgaatc cgatcgcggt cgcgcttccc
     1861 gcgggcgagg aagcgccggt cgtgctcgac atggcaacga cggtcgcggc gtacggcaag
     1921 gtgaagacga aagcccagcg cggcgaaacg atgccggaag gctggatgat cgaccgtcag
     1981 ggaaaaccgc tgctcgatcc gaagcgcgcg agcgagggat ttctccttcc gatcggcggc
     2041 tacaaaggct acggcctcgc gcttgtgatc gggctcctcg ccggcacgct gaacggcgcc
     2101 gcgatgggca aggaggtcgt ggacttcaat gccgacgata cgacgcccac caacaccggt
     2161 cacgcgatcg tcgcgatcaa cgtggaggcg ttccagccgg ttgcggagtt caagcgcgcg
     2221 gtggataccc tcatccgcga tcttcgcaat tcccgcacgc ttccgggcgt cgatcgcgtt
     2281 cgcataccgg gcgagggcag ccatgcggct cgaacggagc gcggccgata cgggataccg
     2341 ttgccgccag cgctgtgcgc gtcgctcgat acgctcgcgc gggagctcgg gatcgcgccg
     2401 ttgccgtgaa gcgccgaacg ggctagcgtg cacaggcttg aacgcaactg ccgtaacggc
     2461 ttgattcgtg agcgatgttc cgcgctctgc cgcagccgac actcctccag gccgaagcgt
     2521 tgaaggaacc ctgtgtcgcc ggcgattact aatgcatgca tggtgggtag gttgatcgca
     2581 tgacattgtc acggaagggg ttttcatgaa gacgatggca cggctggcag gtctctttct
     2641 gacattcgct gcgtgcgcgg cattcgccgc ggaatccaca ccggatgcgc tggtcaagcg
     2701 cacggtggat gaagtgatga gtgtgctcaa ggagaacaag gatcggagga agctcgtcga
     2761 gctcgccgag cagaaggtgt tgccgaactt cgacttcacc gagatgacac gactcgcggt
     2821 ggggcgcgcc tggcgcgagg cgaacgatgc gcagaaaaag gcgctcgaga acgcgtttcg
     2881 cacgctgctc gtgaacacat ataccaccgc gctcatgcag gccgccaaag ccgaccagac
     2941 cgtcgaagtg aagcctgtgc gcgtgcagcc ggggcagaag gaagtggtgg tcaagaccgt
     3001 ggtgaaggag cccggccgac cggcgatccc gatcgactac agcatgcggc gcggcgactc
     3061 gcagtggaag gtgtacgacg tcgtagtgga gaacctgagt ctcgtcacga actatcgcaa
     3121 cacgttcagc tccgaagtcg ccaaaggcgg catcgacgga ttgatcaaga gcctcgaaga
     3181 caagaatcgc aggatcgcgg ccggctgaag cgggtgcgcg gccccgaggg atggaccggt
     3241 cggcggcatg ggcggcttgt cgcacggcgg ccgttcgccg ataatcaact gctcaaaaag
     3301 tgatcacccg ggatgggcag cgtggtccat cctgcccatc cttcccagcg gaccagcgag
     3361 caccaagaga aagaatcgac gctaccatgc gcttgcggag cgaggacgca tacggtcggc
     3421 gctgcatcgg gtcggccggc cggaacgcat ttcaacgatc gacgaagcac cggccggcaa
     3481 ggtcccggcg gcgtttcgac tgtagcctga catgatccgg tttctgtatc cgcgatcgtt
     3541 tcccaggctg ctcgcggtcg gcttcacgtt gatcgctttg cccctcatct tcgccctgat
     3601 cggcagcgcg gtgacggtgg atcagattgc caaccgcagc cagcaggcgg tgtatcgcgc
     3661 ggtgcttgcc acgcagaccg gccgccggct ggccgagctt ttgatcgcca tggagcgcag
     3721 tgcacgacag atggtcattc tggacgaccg caggctgctc gaaacgtacg agtcgaaccg
     3781 caagcatttc gtggcgaccg ctcgggagtt ctcggcgctg cctttcgaca gcgagcagat
     3841 gagcgcgctc ggtgaaatca tgagcggcga gcaggcgatc tataaagcgc tctcgacccc
     3901 gtacgcgacc accgaggagt tgcagaaggc ggtcggcggc ttcgtcgggt tgaccgagca
     3961 cgcgggcgcg atcaccgcgc gcagc
//
LOCUS       DASURG010000259         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_250696, whole genome shotgun sequence.
ACCESSION   DASURG010000259 DASURG010000000
VERSION     DASURG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250696"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..870)
                     /locus_tag="VFY80_04460"
     CDS             complement(292..870)
                     /locus_tag="VFY80_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEX6007190.1"
                     /translation="MSMINYASREINCKLVYYGPGLGGKTTNLEYVYSKVAPDTRGKL
                     ISLATEQERTLFFDFLPVDLGTIRGFKTRFHLYTVPGQVYYNASRRLILKGVDGIVFV
                     ADSQAERMDANIAAMQNLYENLADYGYDPANIPIVLQWNKRDLPTAVPVAELRENLNP
                     DELPEYEAVAVTGEGVFETLKAVSKAVIKTLS"
     gene            complement(874..1377)
                     /locus_tag="VFY80_04465"
     CDS             complement(874..1377)
                     /locus_tag="VFY80_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX6007191.1"
                     /translation="MTPARDGIGSLSETDAAQLTSLLANFVGETQADCAVLCDRSGRL
                     LTLSGDLGAMDQTAFASLVAGDFAASDQLARLLGEDEFSSLYHAGLGRSMYLADVSGY
                     GILAALFNGATTLGMIRLKSRTTVPRLVELFEQIASRSPADSGMGMDENWMSEAEDEI
                     DRLFGEN"
     gene            complement(1374..1718)
                     /locus_tag="VFY80_04470"
     CDS             complement(1374..1718)
                     /locus_tag="VFY80_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007192.1"
                     /translation="MNRDKTIRTLGVVLLVLLALVGLGALVVRDQIARHKRDLFSGHA
                     FRRFAALTHLARMDATIDLVHTLRDFVAREPRSMLRNRGRQILDRMEAALAADHTYRR
                     HAGHGAAGEFAG"
     gene            complement(1785..>2195)
                     /gene="recR"
                     /locus_tag="VFY80_04475"
     CDS             complement(1785..>2195)
                     /gene="recR"
                     /locus_tag="VFY80_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX6007193.1"
                     /translation="CALCANPRRDRTLVCVVEEASDIAAIERTGEYRGLYHVLGGRLS
                     PLEGIGPDELNIKSLLERVRAPEGDTETAVHEVIVATNPSVEGEATALYLQRLVHPLG
                     IRVTRLARGLPVGGDLEYADGVTIAQALNHRREI"
BASE COUNT          429 a          714 c          653 g          399 t
ORIGIN      
        1 cttggtgtcc ttcagcacat catcccaggg cttgaagggg tctttcttgt cgggagttgc
       61 cgcggcattg gcggctggcg caggcgtggg tggtgccttc ggctcggtgt aaacgtgaat
      121 gcagctggcg aacaggagcg atagcgcgag agtgggcgca acgcgtggga gagacatagg
      181 tgtgctccgg ggtgtttaag ttaaccgtcg ctgcgccggg acgagatcca ccgccaagac
      241 gccaagacgc caggaattag acgcgcgccc ccgggaagtg gttgcgcctc gtcaggacag
      301 cgtcttgatg accgccttgc tgaccgcctt caacgtctcg aacacgccct cgcccgtgac
      361 cgcgaccgcc tcgtattcgg gcagctcgtc cgggttcagg ttctcgcgca attcggccac
      421 gggcacggct gtcggcaggt cacgcttatt ccattgcaac acgataggaa tgttggcggg
      481 atcgtagccg tagtcggcca ggttttcgta gaggttctgc atggcggcaa tgttggcgtc
      541 catccgctcg gcctgcgaat cggcgacgaa gacgataccg tcgacgccct tgaggatgag
      601 acgccgactg gcattgtagt acacctgccc cggcacggta tacaagtgga agcgggtctt
      661 gaagccgcga atggtgccga gatcgacggg caggaagtcg aaaaagagcg tccgctcctg
      721 ctccgtagcc agagaaatca gtttgccgcg tgtgtcgggt gcaactttac tgtaaacgta
      781 ttccaggttt gtcgtcttgc cacccagccc gggaccatag tagaccaact tgcagttgat
      841 ctcccggctg gcgtagttga tcatcgacat ccgctagttc tctccgaaca ggcgatcaat
      901 ctcatcctcg gcttcactca tccagttttc gtccatgccc atgccactgt cggcgggcga
      961 acggctcgcg atctgttcga acagctcgac caggcgcggc accgtcgtgc ggctcttcag
     1021 gcggatcata ccgagcgtgg tcgcgccgtt gaagagcgcc gcaagaatac catatccgga
     1081 gacatccgcc agatacatcg agcgaccgag ccccgcgtga tagagcgagg agaactcgtc
     1141 ctcgcccagc aaacgcgcca gctggtcgct cgcagcaaaa tccccggcca ccagggaagc
     1201 aaacgcggtc tggtccatgg cgcccaggtc accgctcagc gtgagcagcc gcccggatcg
     1261 gtcgcacagc accgcacaat cggcctgcgt ctctcccaca aaattcgcca ggagcgacgt
     1321 caactgggcg gcatcggtct cagataacga gccaatcccg tcacgggccg gtgtcatccc
     1381 gcaaactctc cggccgcacc atggccggca tgacgacgat aggtgtggtc cgcagccagc
     1441 gccgcctcca tgcgatccag gatctggcgg ccgcgattgc gcagcatgct gcgcggctca
     1501 cgcgcgacga agtcgcgcag cgtgtgcacc aggtctatcg tcgcatccat acgcgccaga
     1561 tgcgtgagcg cggcaaagcg gcggaacgca tggccgctga acagatcacg cttgtgccgc
     1621 gcgatctggt cgcgcactac cagcgcgccc agcccgacca gggccagcaa caccagcagc
     1681 accacgccca gcgtgcggat tgtcttatcg cggttcataa ccaacccttc gacggcttgc
     1741 ctgcgtaacc ggcatcgtga catgccggcg aggactgcgt aaccctagat ttcccttctg
     1801 tggttcagcg cctgcgcaat ggtgacgcca tccgcatatt ccaggtcacc tccgacaggc
     1861 aatccgcgtg caagacgcgt aacgcgaatt ccgagcggat gaaccagccg ctgaagatac
     1921 aacgccgtgg cctccccttc cacactcgga ttggtggcga cgatgacttc gtgaactgct
     1981 gtttccgtat cgccttccgg cgcacgcaca cgctcgagca gactcttgat gttcagctcg
     2041 tcagggccga tgccctccag cggcgacagc cgcccaccca gtacatgata cagcccgcga
     2101 tactcgcccg tgcgctcaat ggccgcaatg tccgatgcct cttccacgac gcacaccaaa
     2161 gtccggtcac ggcgcggatt cgcgcacaac gcgca
//
LOCUS       DASURG010000260         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_659031, whole genome shotgun sequence.
ACCESSION   DASURG010000260 DASURG010000000
VERSION     DASURG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659031"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..408)
                     /locus_tag="VFY80_04480"
     tRNA            complement(332..408)
                     /locus_tag="VFY80_04480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(372..374),aa:Pro,seq:ggg)
     gene            complement(446..838)
                     /locus_tag="VFY80_04485"
     CDS             complement(446..838)
                     /locus_tag="VFY80_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6007194.1"
                     /translation="METNSHNSSPNSNSQAKAALPPIPAKRYFTIGEVSELCGVKPHV
                     LRYWEQEFTQLKPVKRRGNRRYYQHHEVLLIRRIRELLYDQGFTINGARNRLDELIAE
                     PTRSPRGGRSTAAHLRREIKNVIELLRA"
     gene            complement(789..1172)
                     /locus_tag="VFY80_04490"
     CDS             complement(789..1172)
                     /locus_tag="VFY80_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX6007195.1"
                     /translation="MDAAAIRREAPLKGGTMTLTKAELADLLFEKVGFNKREAKDMVE
                     SFFEEVRHALESGNGVKLSGFGNFQLREKPQRPGRNPKTGEEIPITARRVVTFHASQK
                     LKAMVEQNYDGNEQPQQQSEQQQPS"
     gene            complement(1136..>2918)
                     /gene="pheT"
                     /locus_tag="VFY80_04495"
     CDS             complement(1136..>2918)
                     /gene="pheT"
                     /locus_tag="VFY80_04495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6007196.1"
                     /translation="AVAPTMRDAFPVVLYAGQACPRYCGRLVRGVNAAARTPSWMVNR
                     LERSGVRSISAVVDITNYVMLETGQPLHAFDSSKLEGGIHVRFAGEGESLVLLNGERL
                     TLTPDFLVIADGPKARALGGIMGGAECGVEPHTREIFLESAFFDPDVIAGKSRRLGFG
                     SESAYRFERGVDFSATRLALERATQLVIELCGGEAGPISEAVESLPKRAPVALRVGRL
                     KRVLGVDIGHEQARALLARLGFHCAGDEQVIYATPPAYRFDIAIEEDLIEEVARLHGY
                     DNIPAAAPVAAARMLPAPEARRDAQSVRRLLVARDYQEVVTYSFVARDWEIDFAGNAS
                     PVELANPIASQMSVMRSTLIGSLVNCVSFNENNKQPRVRVFEIGRCFERASDDTQAQP
                     LRIAGVAYGDVVEEQWATPPRAVDFYDIKADVTALIAAESIQFEPAPHPALHPGKSAR
                     VVRSGTTIGWVGELHPRLQQKYDLRRAPVVFELDFEQAVGGGIPAYSEISRFPPVRRD
                     LAFVVDEGLTFEVMLQGLRAAASPIVSEIGVFDVYRGPGLENGKKSLAFRILLQDTRK
                     TLTDTEVECAISQLIQSMQRQFGAKLR"
BASE COUNT          536 a          913 c          901 g          568 t
ORIGIN      
        1 tgaaaatgac cacgacaaac gccgcgagca gcgtgggaag cgagagcctc atcgatgcgt
       61 ccttctttac cgtagcctcg tcgtctgctc caatactatt acgccgatgg gcgcgacggg
      121 ctgttgagct gcgtcaacaa atccgcaaat ttacctcacg ttccttgcgg agcttgagcg
      181 gttcggcaag cgtcgatgcg cgaggcgggg cgctggcgcg ccacgttggg aacggcttgt
      241 ggctgtcggt ttacaagtgg gtttagagat gacccgaaaa ccggcttctt ctgacttttc
      301 taatttgccg cttttatctt ttgcaatctg ttggtcgggg cggtgagatt tgaactcacg
      361 accaccggca ccccatgccg gtacgctacc aggctgcgct acgccccgag gcggaagatt
      421 atagcagcga ccgcacgcaa gcgactcacg cgcgcagcag ctcgatcacg ttcttgatct
      481 cgcggcggag atgcgccgcc gtgctgcggc caccgcgtgg cgatctggta ggttcagcga
      541 tgagctcgtc gagacggttg cgggcgccgt tgatggtgaa gccctgatcg tagagcagct
      601 cgcgtatgcg gcggatgagc aacacttcgt gatgctgata gtagcgccga ttgcctcggc
      661 gcttgacggg cttcagctgc gtgaattcct gctcccaata gcgcagcacg tgcggcttga
      721 cgccgcagag ctcgctcacc tccccgatgg tgaagtagcg cttggcgggt atgggcggaa
      781 gcgcagcctt agcttggctg ttgctgttcg gactgctgtt gtggctgttc gtttccatca
      841 tagttctgct ccaccatcgc cttcagcttc tgggatgcgt ggaaagtgac cacccggcgg
      901 gcagtgatcg ggatctcctc gccggttttg ggattgcggc cggggcgctg cggcttttcg
      961 cgcagctgga agttgccgaa gcccgagagc ttgacgccgt tgccgctttc gagcgcgtgg
     1021 cgcacttctt cgaagaacga ttccaccatg tccttcgcct cgcgcttgtt aaagccgact
     1081 ttctcgaaca gaagatccgc gagctcggcc ttggttaacg tcatcgtccc ccctttcaac
     1141 ggagcttcgc gccgaattgc cgctgcatcg attgaatcaa ctgcgagatg gcgcattcga
     1201 cctccgtgtc cgtcaaggtc ttccgagtat cctgcaataa gatcctgaat gcaagacttt
     1261 ttttgccgtt ttcgagaccc ggtccgcggt agacgtcgaa gacccctatt tcgctcacga
     1321 tgggcgacgc ggctgcacgc aggccctgca acatgacctc aaaggtgagc ccttcgtcga
     1381 ccacgaaagc gaggtcgcgc cgcaccggcg ggaaacggga aatttcgctg taagcgggta
     1441 tgcctcctcc aaccgcctgc tcgaagtcga gttcgaacac gacgggagcg cgtcgcaaat
     1501 catacttctg ttgcagccgc ggatgcaatt ctccgaccca gccgatcgtc gtgccggagc
     1561 gcacgacgcg cgccgacttg ccgggatgca acgcgggatg cggcgcgggc tcgaattgga
     1621 tgctctccgc agcgatgagc gcggtaacgt cggccttgat atcgtagaaa tctaccgccc
     1681 gcggtggcgt cgcccactgc tcttccacga catcgccata agccaccccg gcgatccgca
     1741 gcggttgggc ttgcgtatca tcggaggcgc gctcgaaaca gcgcccgatt tcgaacaccc
     1801 gcacgcgagg ctgcttgttg ttctcgttga aggagacaca gttcacgagg ctgccgatca
     1861 gcgtggaacg catcacgctc atttggctcg cgatgggatt ggcgagctcc accggcgacg
     1921 cgttgccggc aaaatcgatc tcccaatcac gcgcgacgaa actgtaggtc acgacttcct
     1981 gataatcgcg cgcgaccagc aggcgccgga ccgactgcgc gtcacgccgc gcttccgggg
     2041 cgggaagcat tctggccgcg gccacagggg ccgcagcggg aatgttgtcg tagccatgaa
     2101 ggcgcgccac ctcttcgatc agatcctcct cgatcgcaat gtcgaagcga tacgcgggcg
     2161 gcgtcgcgta gatgacctgc tcgtctcccg cgcaatgaaa gccgaggcga gcgaggagcg
     2221 cgcgcgcctg ctcatggcct atgtccacgc ccagcacgcg cttcaggcgc cccacccgca
     2281 atgcgaccgg ggcgcgtttc ggcagcgact ccaccgcttc gctgatcggt cccgcttcgc
     2341 cgccgcagag ttcaatcacg agttgcgtgg cgcgctcgag cgcaagccgt gttgcggaga
     2401 aatcgacgcc gcgctcgaag cgataggcgg attccgatcc aaaaccgaga cggcgcgatt
     2461 ttccggcgat gacatcagga tcgaaaaatg cggactcgag aaagatctcc cgagtatgcg
     2521 gctcgacgcc gcactcggca ccgcccatga tgcccccgag cgcgcgcgcc ttgggcccgt
     2581 cagcgatcac gagaaagtcg ggtgtaagcg tgagcctctc tccgttgagg agcacgagcg
     2641 attcgccctc cccggcgaaa cgcacgtgta tgccgccctc caatttcgat gaatcgaacg
     2701 cgtggagcgg ctggcccgtc tcgagcatga cgtaattcgt aatgtccacc actgcgctga
     2761 tcgaacgaac accgctgcgc tcgagccggt tcaccatcca ggatggtgtg cgcgcggcgg
     2821 cgtttacgcc gcgcacgagg cgcccgcagt agcgcggaca ggcctggccg gcgtagagca
     2881 caacggggaa cgcatcgcgc atcgtcggtg cgactgcg
//
LOCUS       DASURG010000261         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_250785, whole genome shotgun sequence.
ACCESSION   DASURG010000261 DASURG010000000
VERSION     DASURG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250785"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFY80_04500"
     CDS             <1..903
                     /locus_tag="VFY80_04500"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6007197.1"
                     /translation="TALAGVFGAAAAVANLLKLDAVQTNHALGLAGSFAAGTREYLDE
                     GADVKRIHPGKAARDGLLCAEFARRSITGPSKILEGGYGFFQTHAGDEVKWARLFDRM
                     GERFEIESVYFKPYPCCRHYHSIIDGICALRSAHGIRAEEVTHIDVGLYAVGVHGHDH
                     KHCDSLLDAQMSAPCAAALAVVDGKVSASQFLPQSLARPQVQALLERIDTRIDDECER
                     IYPGRRSGTVQIVVRDGRTFNTRVIDPKGEAENPMTDADLEDKLRANCEPILGASRCA
                     ELIERIWHFDKEESIAHFMRALAG"
     gene            1093..1842
                     /locus_tag="VFY80_04505"
     CDS             1093..1842
                     /locus_tag="VFY80_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX6007198.1"
                     /translation="MKVLVTVKRVIDPYVKVRVKSDGTGVETANVKMTMNPFCEIAVE
                     QAVRMKEAGVVSEIVAVSIGVPQCQETLRTAMAMGADRGILVEADAEVQPLAVAKLLK
                     KIVEKESPKLVIMGKQAIDDDSNQCGQTLAALLNWPQAAFTSNITVSGDTADVTREID
                     GGLEKLTVKLPAVMTTDLRLNEPRYVTLPNIMKAKKKPLEVLKPEALGVDIAPRLKTL
                     KVQEPPKRSAGVKVADVQELVTKLKNEARII"
     gene            1844..>2454
                     /locus_tag="VFY80_04510"
     CDS             1844..>2454
                     /locus_tag="VFY80_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX6007199.1"
                     /translation="MAALVIAEHDNRQLKPGVTNAITAAGKAAGDAVHVLVAGHQCEA
                     AAQAAAKVAGVGKVLHADAPHYGGSVAENLAALIVPMAEKYSHILAPATAFGKNVMPR
                     VAGLLDVQQISDICAVETPDTFVRPIYAGNALATVQSSDRIKVLTVRTTAFDPAGEAA
                     SAAPVEKLAPGADTGLTQFKGQELSKSERPELTAARIIVSGGRG"
BASE COUNT          494 a          763 c          805 g          392 t
ORIGIN      
        1 acagccctcg ccggcgtatt cggcgcggcc gctgcggtcg cgaacctgct gaagctcgat
       61 gcggtccaga cgaaccacgc cctcggtctc gcagggagct ttgccgcggg aacccgcgaa
      121 tatctggacg agggcgcgga cgtgaagcgc attcacccgg gaaaagcggc gcgagacggt
      181 cttctctgcg ccgaattcgc gaggcggagc atcaccgggc cgtcgaaaat cctcgagggc
      241 ggctacggtt tttttcagac ccacgccggt gacgaagtga aatgggcgcg cctcttcgac
      301 cgcatgggcg agcgcttcga aatcgaaagc gtttatttca agccctatcc ctgctgccgc
      361 cactatcact cgatcatcga cggcatttgc gcgctgcgca gcgcgcacgg catccgggcg
      421 gaagaggtca cgcacatcga cgtcggtctc tatgcggtgg gcgtccacgg gcacgatcac
      481 aagcactgcg attcgctgct cgacgcgcag atgagcgcgc cctgtgccgc cgcacttgcg
      541 gtcgtggacg gcaaggtgag cgcatcgcag tttctgccgc agagccttgc ccgcccgcag
      601 gtgcaggcgc tgctggaacg catcgacacc cgaatcgacg atgaatgcga gcgtatctat
      661 cccggccgcc gttcgggcac cgtccagatt gtggtgcgcg acggcagaac gttcaacacg
      721 cgcgttatcg atccgaaagg cgaggccgag aacccgatga ccgacgccga cctggaggac
      781 aagctgcgcg cgaattgcga accgatcctc ggcgcatcgc gatgcgcgga gctgatcgag
      841 cgcatctggc atttcgacaa ggaggaaagc atcgcgcatt tcatgcgcgc gctcgccggc
      901 tgaggaccgc tattcgcgct accgaggggg caagcgcatt ccattgggcg tgagccagac
      961 gtcccgtaag cgaatccact ttctgaactc gcttgcgtct ccatcccggc ggccgtttcg
     1021 ctgcccttgc cgatcgggta aaattgtccg cttcgcatat cccgtttcgc tacgtcccct
     1081 cgaggagccc tgatgaaagt actggtcacg gtcaagcgcg tgatcgaccc gtatgtgaag
     1141 gtgcgcgtga aatccgacgg caccggcgtc gagaccgcga acgtgaagat gacgatgaac
     1201 ccgttctgcg agattgccgt cgagcaggca gtgcgcatga aggaagccgg cgtggtgtcg
     1261 gaaatcgtcg cggtgtcgat cggcgtgccg caatgccagg aaacgctgcg cacggcgatg
     1321 gcgatggggg ccgaccgcgg cattctcgtg gaggcggacg ccgaagtgca gccgctcgcc
     1381 gtggcgaagc tcctcaagaa aatcgtcgag aaggagagcc cgaagctcgt catcatgggc
     1441 aagcaggcga tcgacgacga ttccaaccag tgcgggcaga ccctcgccgc gctgctcaac
     1501 tggccgcaag cggcctttac ctcgaacatc actgtgagcg gtgacactgc ggatgtcacg
     1561 cgcgagatcg acggcggtct cgagaagttg accgtcaagc tgccggcggt gatgacgacc
     1621 gatctcagat tgaacgagcc gcgctatgtc acgctgccca atatcatgaa ggcgaagaag
     1681 aagcccctcg aagtgctgaa gcccgaagcg ctcggcgtcg atatcgcgcc gcgcttgaag
     1741 acgctgaagg tgcaggagcc gccgaagcgc agtgccggcg tcaaggttgc ggacgtgcag
     1801 gaactggtga ccaaactcaa gaacgaggcg aggataatct gaaatggcag cgctcgtcat
     1861 tgcggaacac gacaacaggc aattgaagcc cggcgtcacg aacgccatca ccgcagccgg
     1921 aaaagcggca ggcgatgccg tgcacgtgct cgtcgccgga catcaatgcg aggcagccgc
     1981 gcaggcggca gcgaaagtcg ccggcgtcgg caaagtgctg catgccgatg ccccccatta
     2041 cggcggatcg gtggcggaga atctggcggc gctcatcgtg ccgatggcgg agaagtacag
     2101 tcacatcctg gcgccggcga cggccttcgg aaagaacgtc atgccgcgcg tcgccgggtt
     2161 gctcgatgtg caacagatct ccgatatctg tgccgtggaa acgccggaca ccttcgtgcg
     2221 gccaatctat gccgggaacg cgcttgcgac ggtgcagtcc tcggaccgca tcaaggtact
     2281 gacggtgcgc accacggcgt tcgatccagc gggggaagcg gccagcgccg caccggtcga
     2341 gaagctcgcc cccggcgccg acaccggact cacgcagttc aagggacagg agctgtcgaa
     2401 gtccgaacgg cccgagctga ccgccgcgcg catcatcgtt tcaggcgggc gcgg
//
LOCUS       DASURG010000262         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1169325, whole genome shotgun sequence.
ACCESSION   DASURG010000262 DASURG010000000
VERSION     DASURG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169325"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFY80_04515"
     CDS             complement(<1..1252)
                     /locus_tag="VFY80_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX6007200.1"
                     /translation="MPFDAHAALRPFDVGGDLYHYYSLAAAEDAGLRGAARLPYTLKI
                     VLENLVRQHAEGSATSDDVLAVVDWLRHRTSDREIGFKPARVLMPDSSGVPLLGDMAA
                     MRDAMSRLGGDPRKLNPLTHVDFVIDHSVMVDHYGDPEAMARNMALELARNRERYDFL
                     RWGSRAFEHLTVIPPGTGICHQINLEYLARVVWSRAHGGRTIAYPDSVIGMDSHTPMI
                     NALGIVGWGVGGVEGGIAALGEPVAMLIPEVVGCRLTGHPRAGVTATDIVLTITHLMR
                     KQKVIGKFIEFSGPGVDALALPDRATIANMTPEYGATVGFFPVDRETLRYLRLTGRDE
                     KQVSLVEQYCKLQGLWREASTPVPDYTATVELDLATVEPSVAGPRRPNERIALGAAPA
                     TFRGAFPGNDSAAPVKETDYALRNG"
     gene            1409..1957
                     /locus_tag="VFY80_04520"
     CDS             1409..1957
                     /locus_tag="VFY80_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX6007201.1"
                     /translation="MRVAILVAEGFEQSEMTEPKKALEQNGAQTAIVSPARGEVEGWK
                     HFDKGDRFRVDVPLERANADEFDALVLPGGVANPDQLRIQPKAVQFIRAFFEAGKPVA
                     VICHGPWTLIEANVVRGRKMTSWPSVKTDLMNAGANWVDQEVVVDNGLISSRKPADLP
                     AFNRAMVGEFSKAKHQAALAAR"
     gene            complement(2020..2256)
                     /locus_tag="VFY80_04525"
     CDS             complement(2020..2256)
                     /locus_tag="VFY80_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007202.1"
                     /translation="MQAILDYLKLGGDRLDTEIAAATGLPLDSVRSNVTRLQKRGEVM
                     VCRSIRYLDGQEVDSMLCRISGYVPPRAPGRKPK"
BASE COUNT          463 a          777 c          852 g          452 t
ORIGIN      
        1 cgccgttccg taaagcgtaa tccgtttcct tgaccggtgc ggcgctgtcg ttgcctggaa
       61 acgccccgcg aaaggtcgcg ggggccgcgc ccagcgcgat gcgttcgttg ggccggcgcg
      121 gccccgctac gctcggctcg acggtggcca gatcgagttc aacggtcgcg gtgtaatcgg
      181 ggacgggggt cgaggcctcg cgccatagcc cctggagctt gcagtactgc tcgaccaatg
      241 acacctgctt ctcgtcgcgc ccggtgagcc gcaggtaacg gagcgtctcg cgatcgacgg
      301 gaaagaaccc gacggtggcg ccgtactcgg gcgtcatgtt ggcgatcgtc gcacggtccg
      361 gcagcgcgag cgcatccact cccggcccgg agaactcgat aaacttcccg atcactttct
      421 gtttgcgcat gagatgcgtg atcgtgagca cgatgtcggt ggctgtcaca cctgcacgcg
      481 gatggccggt gaggcggcag ccgacgactt ccgggatgag catggcgacc ggctcgccga
      541 gcgccgcgat gccgccttcg acgccaccga cgccccagcc aacgatgccg agcgcgttga
      601 tcatcggcgt gtggctgtcc atgccgatga cggaatcggg ataagcgatc gtgcgacccc
      661 cgtgtgcgcg cgaccacacc acgcgcgcga gatattccag attgatctga tggcagatgc
      721 cggttccggg cgggataacg gtcagatgtt cgaatgcacg actaccccaa cggaggaagt
      781 cataacgttc ccggttgcgc gcgagctcga gcgccatgtt gcgcgccatc gcttcggggt
      841 caccgtagtg atccaccatg acagagtggt cgatgacgaa gtcgacgtgc gtgagaggat
      901 tcaatttgcg cggatcgccg cccagtcgcg acatcgcatc gcgcatggcc gccatgtcgc
      961 ccagcagcgg cacaccggac gaatccggca tcagcacgcg tgcgggcttg aatccgattt
     1021 cgcggtccga cgtgcgatgg cgcagccagt cgaccaccgc cagaacgtcg tcgctcgtcg
     1081 cgctgccttc ggcgtgctgc cggaccaggt tttcgagcac gatcttcagc gtatagggca
     1141 ggcgcgcagc accgcgcagc cctgcgtcct ccgctgcagc aaggctgtaa tagtggtata
     1201 gatcgccgcc gacatcgaac ggtcgcagcg ctgcatgggc atcgaatggc atttctgaag
     1261 tccctgtaat cgggccggac ggccggggaa ggccgctatc ttagcgcacc acgctcgcgg
     1321 gttcgaaggg cacgcttatt gcgaaggata ctccgcgtcg catgagcggc cacaatttcg
     1381 atatggaggt cagtctatgc tggaaggtat gagagtcgcc attctcgtcg ccgaaggatt
     1441 cgagcagtcc gaaatgacgg agcccaagaa agcgctcgaa cagaacggcg cgcaaacagc
     1501 tatcgtgtcg cctgcgcggg gcgaggttga gggctggaag cactttgaca agggagatcg
     1561 cttccgggtc gacgtgccgc tcgaacgggc gaacgctgac gagttcgacg cgctggtgtt
     1621 gccgggcggc gtcgcaaatc ccgatcagct acgcatacag ccgaaagccg tccagttcat
     1681 cagggcattc ttcgaggcgg ggaagccggt cgcggtcatc tgccacggcc cttggacgct
     1741 catcgaggcg aacgtcgtgc gcgggcggaa aatgacgtcg tggccttcgg tcaaaaccga
     1801 tttgatgaac gcgggtgcga actgggtcga ccaggaggtg gtggtcgaca acgggctcat
     1861 ttcgagccgc aagccggcgg acctgcctgc gttcaaccgg gcgatggtgg gcgagttttc
     1921 gaaggcaaag caccaggcgg cgctcgctgc gcgctgagcc tgtaaaagct gcaccgcggt
     1981 tgtaagttta tcttggtgcg gccccggcag tgcgcgaggt tacttgggtt tgcgtccggg
     2041 ggctcgcggc ggcacgtagc ccgagatgcg gcacagcatg gagtcgactt cctggccgtc
     2101 caggtagcgg attgagcggc ataccatgac ttcaccgcgc ttctgcaggc gcgtgacgtt
     2161 gctgcgcacg gagtcgagag gtagaccggt cgccgctgcg atttctgtat cgaggcggtc
     2221 gccgccaagt ttgaggtagt ccagtatggc ctgcatgccg atgctcccgt tgcgtggatg
     2281 ggtcggtccg ggcgaacgcc cgaagccggg ggagggcgaa tgatagcgta aatccacgca
     2341 aacttcgaat tcggggccgg tatgcgacat tgcttgtcgc gccgcgagcg ttcgacatac
     2401 catgccgcca acccagcgaa aaaaagcggt tcctccgcac gttcgcaatc gaattggcta
     2461 cgcccgaatc ccgcttgacc ccgagtgcgg ccgcgaccgg ccgcgactcg caccgccagg
     2521 tgctcgccat ggcgggcccc atca
//
LOCUS       DASURG010000263         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1477027, whole genome shotgun sequence.
ACCESSION   DASURG010000263 DASURG010000000
VERSION     DASURG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477027"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /gene="rpsB"
                     /locus_tag="VFY80_04530"
     CDS             complement(<1..165)
                     /gene="rpsB"
                     /locus_tag="VFY80_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6007203.1"
                     /translation="MPVTMREMLEAGVHFGHQTRYWNPRMAEYIFGHRNKIHIINLEK
                     TLAMYQGALKF"
     gene            423..1229
                     /gene="map"
                     /locus_tag="VFY80_04535"
     CDS             423..1229
                     /gene="map"
                     /locus_tag="VFY80_04535"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX6007204.1"
                     /translation="MAVHIKSSEEIQKMRVAGRLAAEVLDFIAPHVRPGVTTGELDRL
                     CHEYMVDQQRVTPAPLNYAPPGYQPFPKSICTSVNHVVCHGVPGDKKLKPGDIINIDI
                     TVIKDGYHGDTSRMFYVGQPSIQAKRLCDVTYECMWRGIEAVKPGATLGDIGHAIQSH
                     AEANGYSVVREFCGHGIGARFHEEPQILHYGRPGTGLRLEAGMTFTIEPMINAGKADI
                     RQLADGWTIVTKDHSLSAQWEHTVLVTQTAHEVMTVSAGTPAKPALGRVT"
     gene            1249..>2961
                     /gene="glnD"
                     /locus_tag="VFY80_04540"
     CDS             1249..>2961
                     /gene="glnD"
                     /locus_tag="VFY80_04540"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX6007205.1"
                     /translation="MSGLTVPSGSRLAPLASEWKRALASERETLRRGFFRHPVAGRLL
                     ARHSQLVDRQLREVWSKHEMPRDWALAAVGGYGRGQLFPCSDIDLLILLPTAADMDVQ
                     RRLETFLGALWDIGLEVGHSVRTIDECVELAQQDLTVQTSLLEARLVRGDRQLFRRFV
                     TRLDALLDPAEFLQAKQLEQQQRHARYQESNLEPNIKESAGGLRDLQTILWIARAGGI
                     GKTWRELARRGVITVQEAHEIQRHEAFLQSLRVRLHYLAARREDRLIFDYQSALAREL
                     KLHDKPHRLASEQLMQRYYRAAKAVSELNAILLQNLKARITPSEDQAYHPINARFGMR
                     DELLEAIDEDLFEREPGAMLEAIRVLQEHHELKGIGAPTLRALWRARRHIDPEFRRDP
                     VNRRLFIEILRSPTHVERALRRMNQSGILGRYVPAFGRIVGQMQHDLYHVYTVDEHIL
                     RVVRNLRRFSIPELAHEFPLCSRLMSNFARPEVLYLAALFHDIAKGRGGDHSQLGKTD
                     AQRFCTAHALAHEDTELVVWLVEHHLVMSTTAQKQDLSDPAVIQAFAEKVRDQRRLAA
                     LYLLT"
BASE COUNT          544 a          961 c          917 g          539 t
ORIGIN      
        1 gaacttgagc gccccctggt acattgccag agtcttttcg agattgataa tgtggatctt
       61 gttgcggtgg ccgaagatgt actccgccat cctcggattc cagtagcggg tctgatgtcc
      121 gaaatggacg cccgcctcca gcatttcacg catcgtgacc ggcatgtggt actcctttaa
      181 gggttgagcc gccatccgct ggaaacccgc gctcgacgcg acaggcaccc ttcgcttttc
      241 tcgcggatgt gtgttttcgc tgccgaaagg gcaagctaac ttgctagttt actacatgaa
      301 tgtcgcgcct tcccagtggg aagtcgctcg agccgccgcg gcaggcgcgc tctgatacaa
      361 tcccctttcc cgaaaaacca agcacttgga atcttctgct gcggtgaagt tccgtaccgt
      421 ctatggcggt ccacatcaaa tcctctgaag agatccagaa gatgcgtgta gccggtcggc
      481 ttgcggcgga ggtgctcgat ttcatcgccc cgcacgtgcg ccccggcgtg acgaccggcg
      541 agctcgaccg tctgtgtcat gaatacatgg tggaccagca gcgggtgacg cctgcgccgc
      601 tcaactatgc gccgcccggc tatcagccct tccccaagtc gatttgcacc tcggtcaacc
      661 acgtggtctg ccatggcgtg ccgggcgaca agaagctcaa acccggggat atcatcaaca
      721 tcgatatcac cgtcatcaag gacggctatc acggcgacac gagccgcatg ttctacgtcg
      781 gccagccgtc gatccaggcg aagcgcttgt gcgatgtgac ctacgaatgc atgtggcgcg
      841 gcatcgaggc ggtgaagcca ggcgcaacgc ttggcgatat cggtcatgcg atccagagcc
      901 atgccgaggc gaacggctac agcgtggtgc gcgagttctg cggccatggc atcggggcgc
      961 gtttccacga ggagccgcag atcctccact atggacgccc cggcaccgga ctgcggctcg
     1021 aggcgggcat gacttttacg atcgagccca tgatcaatgc cggcaaggcc gacatccgtc
     1081 agctcgccga cggctggacg attgtcacca aggatcacag tctctccgcg caatgggagc
     1141 acaccgtact ggttacgcag acggctcatg aggtgatgac cgtctcggcc ggcacgccgg
     1201 cgaagccggc cctcggccgc gtcacgtgag cggcggtttg cggcattcgt gagcggattg
     1261 accgtcccgt caggctccag gctcgctccg ctcgcgagcg aatggaagcg cgcgctggcg
     1321 tcggagcgcg aaaccctgcg ccgcggtttc tttcggcatc cggtggcagg ccgactgctc
     1381 gcgcggcaca gccagctcgt cgatcgccag cttcgcgaag tgtggtcgaa gcacgagatg
     1441 ccccgcgact gggcgctcgc tgcagtcggc ggttacgggc ggggccagct ttttccctgc
     1501 tcggacatcg atctgctgat cctgcttcca actgcggcgg atatggacgt ccagcgtagg
     1561 ctcgagacct ttctcggcgc gctgtgggac atcgggctcg aggtcgggca tagcgtgcgc
     1621 acgatcgatg aatgtgtcga gctcgcgcag caggacctca cggtccagac gagcctgctc
     1681 gaggcccgcc tcgttcgcgg cgaccggcag ctcttccgcc ggtttgtcac ccgcctggat
     1741 gcgctgctgg atccggccga gtttctccag gcgaagcagc tggaacagca gcagcgccat
     1801 gcccgctacc aggagtcaaa cctcgagccg aacatcaagg aaagcgcagg cggcctgcgt
     1861 gatctgcaga ccattctgtg gatcgcgcgc gcgggaggca tcggcaagac atggcgcgag
     1921 ctcgcgcgcc gcggcgtgat cacggtccag gaagcacacg aaatccagcg ccacgaggct
     1981 tttctgcagt ccctccgcgt acgcctccat tacctcgctg cgcgccgcga ggaccggctc
     2041 atcttcgact atcagtcggc gctcgcgagg gaactcaagc tgcacgacaa accgcaccgc
     2101 ctcgcaagcg agcagctcat gcagcgttac tatcgggccg cgaaggcggt ctccgagctg
     2161 aacgccatcc tgctgcagaa cctgaaggcc cgcatcacgc cctcggagga tcaggcctat
     2221 caccccatca atgcgcgctt cggcatgcgc gacgagctcc tggaggcgat agacgaagat
     2281 ctcttcgagc gcgagccggg cgccatgctg gaagcgatac gggtcctgca ggagcaccac
     2341 gagctcaagg gcatcggcgc gccgacgctg cgcgcgctat ggcgtgcgcg caggcatatc
     2401 gatccggagt ttcggcgcga tccggtcaac cggcgcctct tcatcgagat cctgcgcagc
     2461 ccgacgcacg tggaacgtgc gctgcggcgc atgaatcagt ccggtatact gggacgttac
     2521 gtgccggcct tcggccgcat cgtagggcag atgcagcatg atctctatca tgtctatacc
     2581 gtggacgagc acatcctgcg cgttgtccgt aacctgcggc ggttctcgat tcccgagctt
     2641 gcacacgaat ttccgctctg cagccggctt atgagcaatt tcgcgcgccc cgaggtgctc
     2701 tacctcgcgg cgctctttca cgacatcgca aaagggcgcg gcggagacca ctcgcagctc
     2761 gggaaaacgg acgcgcagcg cttctgcacg gcacacgcgc tcgcgcacga agataccgag
     2821 ctcgtggtgt ggctggtcga gcaccatctc gtcatgtcca cgaccgcgca aaagcaggac
     2881 ctgagcgacc ccgccgtgat ccaggcgttt gccgagaaag tgcgggacca gcgacgactc
     2941 gcggcgctct atctgctcac c
//
LOCUS       DASURG010000264         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_251003, whole genome shotgun sequence.
ACCESSION   DASURG010000264 DASURG010000000
VERSION     DASURG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251003"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFY80_04545"
     CDS             complement(<1..548)
                     /locus_tag="VFY80_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007206.1"
                     /translation="MTMKAFDRNAFALLCGAIVVLLSPGSASAADATLISKADNYPHK
                     PIRVIDPFAAAGASDIVIRLVGQKLTERYGQPVIVDNRPGAGGNLAAELAAKATPDGY
                     TLFNVVVFSLAPSVSLYPKMSVDPMKDFAYISLMAGGSYVIVAHPSCPAKTIPELVNL
                     AKQQPGKISYGSSGVGGPVHIAA"
     gene            complement(709..1587)
                     /locus_tag="VFY80_04550"
     CDS             complement(709..1587)
                     /locus_tag="VFY80_04550"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX6007207.1"
                     /translation="MDRAPKALTREQLARFHADGGIAPVQALDPQEAARYRNCLESFE
                     RETGTLLMKHPARPTIWSKAHLLFTWADAIVRHPRILDAVEDVIGPNIRVFHTNVFVK
                     EPHSPEFVTWHQDGAYNGVSPPEVLTTWVALTAATPEMGCMKMLKGSHKHGLRVHVDR
                     KSAGNLLTRGQQIERLENVEVIDLALRPGEMSIHHSYAVHSSEPNRSNERRIGLMTTY
                     VPTHCRYIGSGSARLRCALVRGRDDYGHFDEEPQPVIDFGMAERAEHLESCTRYIANV
                     TSSANKQLPNAVTVNA"
     gene            2025..2939
                     /locus_tag="VFY80_04555"
     CDS             2025..2939
                     /locus_tag="VFY80_04555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007208.1"
                     /translation="MTRHVLVTGALGFLGYPALLAAAGEGWDVTGLDLLQPPQPIPGA
                     HFVRGDITNVHQIYRVLRERAIDTIIHTGGISGPMLERDDPFLVCSANVIGTINLLEA
                     ARVNGVRRFVFLSSAHAYGDTPPPPVTEEAPFRTRDIYGASKASGDLLLRAYREQYKL
                     DAVALRISNGYGPRRVTRDAIRIMLQDAIAGRPTSLDFGGGYGRTYVYVKDAVDAIMA
                     AVKAPSVAQPAYNITGTEFVSMQHIADIVRKLFPKASITMAEGVDPLGYRREQLDVSA
                     AARDLGWAPQWTLERGIVDYAQWLRANP"
     gene            3011..>3577
                     /locus_tag="VFY80_04560"
     CDS             3011..>3577
                     /locus_tag="VFY80_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007209.1"
                     /translation="MGELSGKVTVITGASSPTGIGQAIARRYAAAGASVYLMAEGTEH
                     ELRAGVDACRKVSTDKDARFEFGVHDLAKPCVPEAMIAEVAHLFGRIDVLVNNAGVRA
                     RLKFEEVSRDAFDNAVAVNIAAPFFASQAAVPLMRRQGGGRIIHIASQMGVVTHERRS
                     VYGLTKAALIHLTKAMAFELAPDGIIVNA"
BASE COUNT          651 a         1123 c         1119 g          684 t
ORIGIN      
        1 gccgcaatat gcacggggcc gccgacgccg gaagagccat agctgatctt ccccggctgc
       61 tgcttcgcga ggttcacgag ctcggggatg gtcttcgcgg gacacgaagg gtgcgcgacg
      121 atcacatacg acccgcccgc catcagcgag atatacgcga agtccttcat cggatctacg
      181 ctcatcttcg gatacaggct gacgctcggt gcgagcgaga acacgacgac gttgaagagc
      241 gtatagccgt ccggcgtcgc cttggcggcg agctccgccg ccagattgcc gcccgcgccc
      301 gggcggttgt cgactatgac cggctggccg tagcgctccg taagcttctg gccgacgagc
      361 cgtatcacga tatcgctcgc gccggcagcc gcgaacgggt cgatcacgcg tatcggcttg
      421 tgaggatagt tgtcagcctt cgaaatgagc gtcgcatcgg cggccgacgc ggatcccggt
      481 gacaggagca cgacgatggc gccgcaaagt agcgcgaatg cattgcggtc gaatgctttc
      541 atggtcatac cccgagtttc gttgagctgt accgtgctgg actcggcaac cttcgccaac
      601 gccaggcttg ccgcatctgc gcttcgagtg cggctcgacg gatcaggtat gcgagatttg
      661 caatgcgcaa aagtagcggc tcgcctgatt ttcgtgcggg cgtcaggctt atgcgtttac
      721 cgtgactgcg ttcggtagct gcttgttcgc actggacgtc acgttcgcga tatagcgtgt
      781 gcacgactcg agatgctccg ctcgctctgc catgccgaag tcgatcacgg gctgcggctc
      841 ttcgtcgaaa tggccgtaat cgtcgcgacc ccgtacgagc gcgcagcgca accgcgcgct
      901 ccccgagcca atgtagcggc aatgcgtagg cacgtaagtc gtcatcaggc ctatgcggcg
      961 ctcgttggag cgattgggct cggatgagtg caccgcgtac gagtggtgta tcgacatctc
     1021 gcccggccgc agcgcgagat cgatgacttc gacattctcg agccgctcga tctgctggcc
     1081 gcgcgtgagc agatttcctg cgcttttccg gtcgacgtgc actctcaagc catgcttgtg
     1141 gctgcccttc agcattttca tgcaccccat ctcgggcgtc gcggcagtca gcgcaaccca
     1201 ggtcgtcaat acctcgggcg gcgatacccc gttataggcg ccatcctggt gccacgtgac
     1261 gaattcgggc gagtgcggct ctttgacgaa cacattggtg tgaaacacgc ggatattcgg
     1321 gccaattacg tcctcgacgg catccagaat acgcggatgc cgcacgattg cgtccgccca
     1381 ggtaaacagc agatgtgcct tggaccagat tgtcggccgc gccggatgct tcatcagcag
     1441 cgtgcccgtt tcgcgctcga agctctcgag gcaattgcgg tagcgcgcgg cctcctgtgg
     1501 atcaagtgcc tgcacggggg cgataccgcc gtccgcgtga aagcgagcaa gttgctcccg
     1561 agtgagtgct ttgggtgcac ggtccatcgg tgctgttcct tgaatggagc ccgcacgcat
     1621 gcatcgtcgc gcgtttaagg tgacgataag tgcagccggg tcaggtgtca atacacagac
     1681 ttcccaacgg gatatgaacc cgatcggcgc agatccccag tggcgagcgg ccttcccccc
     1741 aagggagaat ccacacgggc tcctgcgcct aggcaaacat ccaccgggtg accaggacgg
     1801 cctcaggcgt ctcgaccaca tcaacgcggg cgcgagtcaa tacgcactgc gtcgagcact
     1861 tgaatccgta cctgaccttc caccgcctgt gcatgctctc ccgaaccatc gacttcaagg
     1921 ttcccggcga atgcccgcgg cgactttgat atgatcggta gttcctcact cgcttctctc
     1981 ggattgcgct gcgcgatagg agcgccacgg aaagcatttc acccatgacg cggcacgtgc
     2041 tcgttaccgg cgcgctcgga tttctcgggt atcccgcgct gcttgcggca gccggcgaag
     2101 gctgggacgt gaccggtctc gatttgctgc aacccccgca gccgattccc ggcgcgcact
     2161 tcgtacgcgg cgacatcacg aacgtgcacc agatctatcg cgtgctacgc gagcgcgcca
     2221 tcgacaccat cattcacacc ggcggcatct cgggtcccat gctcgagcgt gacgatcctt
     2281 ttctggtgtg cagcgcgaac gtgatcggca cgatcaatct gctcgaggcg gcgcgcgtga
     2341 atggcgtcag gaggttcgtg ttcctttctt ccgcacatgc ttatggcgac acgccgccgc
     2401 caccggtgac ggaagaagcg cccttccgga cgcgcgacat ctacggtgcg agcaaggcga
     2461 gcggcgacct gctgctccgc gcgtatcggg agcagtacaa gctcgacgcc gtcgcgctgc
     2521 gcatttccaa cggctacggt ccgcgccgcg tcacacgcga tgcgatacgc atcatgctac
     2581 aggatgcaat cgcggggcgt ccgacgtcgc tcgatttcgg cgggggatac gggcgcacct
     2641 acgtgtacgt gaaagacgcg gtcgacgcca tcatggctgc agtcaaggca ccgtcggtcg
     2701 cgcagccggc ttataacatc acgggcaccg aattcgtgtc gatgcagcac atcgcagaca
     2761 tcgtgcgcaa gctgttcccc aaggcgagca tcaccatggc tgaaggcgtg gacccgctcg
     2821 gatatcgccg cgagcagctg gacgtgtcgg ccgcggcacg cgatctcggc tgggcaccgc
     2881 aatggacgct cgagcgcggc atcgtcgact atgcgcagtg gctgcgcgcg aatccctgac
     2941 gtaaagtcat gccagctttc gtgctcgtga agtggcactg ttgtttattc cctgacgatt
     3001 aggagcgcgc atgggtgagc tgtccggcaa ggttaccgtg atcacgggag cttcgagccc
     3061 caccggcata gggcaggcga tagcgcggcg ctacgccgcc gctggagcat cggtctacct
     3121 gatggccgaa ggcacggagc atgagctacg cgcaggcgtc gatgcgtgcc gcaaggtcag
     3181 taccgacaag gatgcgcggt tcgaattcgg cgtgcacgac ttggcgaagc cctgcgtgcc
     3241 cgaggcaatg atcgccgaag tcgcgcatct cttcgggcgc atcgacgttc tggtcaacaa
     3301 tgcgggcgtg cgtgcgcggc tcaagttcga agaagtctcg cgcgatgcgt tcgacaatgc
     3361 ggtcgcagtc aacatcgcgg caccattctt tgcgtcacag gcagccgtgc cgttgatgcg
     3421 tcgccagggt ggtgggcgca tcattcacat cgcaagccag atgggtgtcg tcacgcacga
     3481 acggcgctcg gtttacgggc tcacgaaggc cgcgctgatc catctcacca aagcgatggc
     3541 gttcgagctc gcgcccgacg gcatcatcgt caacgcg
//
LOCUS       DASURG010000265         8486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_556804, whole genome shotgun sequence.
ACCESSION   DASURG010000265 DASURG010000000
VERSION     DASURG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556804"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFY80_04565"
     CDS             complement(<1..735)
                     /locus_tag="VFY80_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX6007210.1"
                     /translation="MLLLTTACETRAPVKELSPPPGSAAPAAPAPVPHPIAPPSTQRV
                     PTASGAALRPAAWNDLPAWRDEDHAAAWEALRTSCSVLAHRDAWRSVCSLAFQSERPD
                     RAKARRFFETHFAPYQLVNADGRAEGLITGYYEPLLRGSRQRSARYRHPVYGVPDDLL
                     VVDLGELYTELKGKQLRGRLDGRRIVPYYDRAGIEGGLAPVRGREIAWVEDPIELFFL
                     QIQGSGRIELENGSTLRVGFADQNGHP"
     gene            complement(1005..1385)
                     /gene="apaG"
                     /locus_tag="VFY80_04570"
     CDS             complement(1005..1385)
                     /gene="apaG"
                     /locus_tag="VFY80_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX6007211.1"
                     /translation="MPDKKHDIRVSARSAFIPEQSDPDRKRYVFAYTITITNTGNIAA
                     QLLSRHWIITDADNQVQEVRGAGVVGEQPLLRPQESFEYTSGAAIATPVGTMRGSYQM
                     VSEEGVKFDVPIPEFTLAVPRILH"
     gene            1487..2149
                     /gene="rpe"
                     /locus_tag="VFY80_04575"
     CDS             1487..2149
                     /gene="rpe"
                     /locus_tag="VFY80_04575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX6007212.1"
                     /translation="MPYRIAPSILSADFARLGEEVRGVVAAGADIVHFDVMDNHYVPN
                     LTVGPLVCAAIRPMIQVPIDVHLMVKPVDRIVPDFAEAGANIISFHPEATDHVDRTIG
                     LIKSCGCKAGLVLNPATPLTWLDHVIGELDLVLIMSVNPGFPGQAFIPEALNKLSAAR
                     RRIEESGREIWLEVDGGVKIDNVREIAQAGADTFVAGSAIFGTQDYRATIAAMRAELK
                     RA"
     gene            2150..2863
                     /locus_tag="VFY80_04580"
     CDS             2150..2863
                     /locus_tag="VFY80_04580"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX6007213.1"
                     /translation="MEITARMHRAESRFPLRVKAVLIDLDGTLLDTIPDLAVAANLML
                     EALGEPPLDAEVIRTFVGKGIPNLVKRTLARDLDGEVEESRFERALPVYERCYASVNG
                     RHTTIYPGVTDGLERLRQAGFPLGCVTNKAMRFTRPLLEQTGLARYFSEVVAGDTLPR
                     KKPDPEPLLYACERFGVSPADMLMIGDSLNDAQAARAAGCPVFCVTYGYNEGRDVREL
                     DVDAIVARLDEAVRLIEKV"
     gene            3006..4481
                     /gene="trpE"
                     /locus_tag="VFY80_04585"
     CDS             3006..4481
                     /gene="trpE"
                     /locus_tag="VFY80_04585"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX6007214.1"
                     /translation="MTEDEFARLASAGYNRVPLVLETFADLDTPLSIYLKLANQPYSY
                     LLESVVGGERFGRYSFIGLAADARIEVHGRACREYRGGRVVSEATSEDPLAYVDAYLA
                     RFNVAQDARLPRFCGGLVGYFGYDTVRYIEKRLASVDKPDPLGTPDILLLVSEEIAIV
                     DNLSGKLSLVVYAEPGAPGAYAKARGRLRELLGKLREPVPIPPDVSTTSEPAVSGFGE
                     RPYHAAVERAKRYIYDGDIMQVVPSQRMSKRFRATPMALYRALRTLNPSPYMFYFDFE
                     DFHVVGASPEILVRLEGDTVTVRPIAGTRPRGATAAEDAALSEELLSDAKERAEHIML
                     VDLGRNDVGRVAQTGTVRVTEQMVIERYSHVMHIVSNVEATLKPGLHAIDVLRATFPA
                     GTVSGAPKVRAMEIIDELEPVKRGVYAGAVGYLGFHGNMDVAIALRTAVIKDGVLHVQ
                     AGGGIVADSEPHAEWQETQNKARALLRAAEMAESGLDTRLP"
     gene            4526..5629
                     /locus_tag="VFY80_04590"
     CDS             4526..5629
                     /locus_tag="VFY80_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEX6007215.1"
                     /translation="MRFRVLATVVRNALVRLEIMANRLLRREFVPDGPGIFHIETSGA
                     CNLKCRFCAYEKKSAPKVSMPNDLFFDCVAQAVALGYTRFDLTPSTGDVFMDKRLFDK
                     FSYLEDHPGVEEYSFFTNLTIPKPEALARLIALHKLRQLTISIYGHDLASFIAITKST
                     ENVYRRLLANLDALLALKERWPFGVAIGFRSTFDVPGEDASELMQALARYRDAGIPVR
                     PSHGVYNNWGGYISQADVAGLDMHITSTELTYKYGACVKLFDSVQVMASGVVNACACR
                     DVDATLRIGNARTTRLKDILSERNAEYMRIIDEQQRGEFRPVCASCDFYKSVYHQRSE
                     YRRSRAPMQTVDDFKAKLGASRQSDSGQEKAAP"
     gene            complement(5780..6409)
                     /locus_tag="VFY80_04595"
     CDS             complement(5780..6409)
                     /locus_tag="VFY80_04595"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX6007216.1"
                     /translation="MATLIASIMRRVRAAPWVRVIAGGALLVSGNAVAQIDDGASEIE
                     VKAAFVYKFMGYVEWPRHSLAADAPLVIGVAGAEDIAGELSRIVTARTIQGRSVTVRP
                     INAPAELNGVQVLFIGADAVSRMPRLIEAARERPILIVTDAPDGLEHGAMINFVRVKR
                     RVQFEIAVAPAERAGLTLSSRLLAVALRVKKGGLEMERLIARLERPLSA"
     gene            complement(6397..8241)
                     /locus_tag="VFY80_04600"
     CDS             complement(6397..8241)
                     /locus_tag="VFY80_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX6007217.1"
                     /translation="MALVLGAAAAPAGARELADLSLEELANIEVTSVSRRAERLSEAP
                     ASIFVITHEDIRRSGATSLPEALRLAPNLQVARVNSSSYAISARGFNNSVGNKLLVLI
                     DGRTVYTPLFSGVFWDAQDVMLEDVERIEVISGPGATLWGTNAVNGVINVTTRPASAT
                     QGALASLGGGNRERGGAVRYGAPLRNDGHFRLYGKFFDRDGTRRADGTSLRDSWDKGQ
                     FGFRADWGGRDRAFTLQGDAYHGDLGEAPGRTKVEGANLLARWNQQLGDGSNLRVQGY
                     YDRSERDQPGVFREELDTFDVELQHALKARGAHTVVWGAGYRYANDRVGNSAVLAFLP
                     AERSLDWWNFFVQDEIALGATLALTLGARVEGNPYTGTELLPGARIAWKPAPDQLVWT
                     ALTRAVRSPARLDREFFIFVPPLPLNGGPNFRSEVSNVIEVGYRAQPSSRYSYSVTAF
                     HHDHDHLRSVEPAPSGGFVLENRIEGSTYGVEGWAHYRVSDRWRLSGGGVLLKQALRL
                     KPDSRDVAGIAALGNDPAHQWILRSAFEPTARHALDVSLRRVGALPNPNVPSYTALDA
                     RWGWQVQRDLEVSVTLTNLFDRRHPEFGAPATRGEVERGVFFKMIWQH"
BASE COUNT         1539 a         2735 c         2673 g         1539 t
ORIGIN      
        1 gggatgcccg ttctgatccg caaatcccac gcgcaaggtg ctgccgtttt caagctcgat
       61 gcgccccgag ccctggatct gcaggaagaa gagctcgatg gggtcctcca cccatgcgat
      121 ttcgcggccg cgcaccggcg ccagaccgcc ttcgatcccg gcccggtcgt agtagggcac
      181 gatgcgccgg ccatcgagac ggccgcgcaa ctgcttgccc ttgagttcgg tatagagctc
      241 accgagatca acgacgagaa gatcgtccgg cacaccatag accggatgac ggtagcgtgc
      301 cgaacgctgg cggctgcctc ggaggagtgg ctcgtaatag ccggtgatga ggccctcggc
      361 gcggccatcc gcgttcacga gctggtaggg ggcgaaatgc gtctcgaaga aacggcgtgc
      421 cttcgcccga tcgggacgtt cggattgaaa cgcaaggctg cagacgctgc gccaggcgtc
      481 gcggtgcgcg agcacgctgc agcttgtgcg caacgcttcc catgcggcgg catgatcttc
      541 atcgcgccag gccgggagat cgttccacgc tgcggggcgc agcgccgcgc cgctcgccgt
      601 ggggacacgc tgcgtgctcg gaggtgcgat gggatgcgga acgggagccg gcgcggcggg
      661 cgcggcggag cctggcggcg gggatagctc tttgaccggc gcccgggttt cgcacgcggt
      721 agtcagcaag agcacggcag cggcgagcca gcgggcgtcg aacgtttgga aattcgaccg
      781 gtgttcgtta aagtgcccca ttttgctgcg gcagtatagc gttcgcgatg gaatccctgt
      841 tgtggctgct actcgaagcg gctgtggctc ttgcgcttct cgtcctcatc gtctggtgga
      901 cttggccgcg gcggaaaccc gatgacgaga acgaccgggg tggctcctga cccgcgcgca
      961 tcgcatgccg ttatgaaaga accgtgccgc ggatcggctc ttcttcagtg gagaatgcgc
     1021 ggcacggcaa gtgtgaattc gggaatgggg acgtcgaact tgacgccctc ctcgctgacc
     1081 atctggtagc ttccgcgcat cgttccaacc ggcgtcgcga tggcggcgcc gctcgtgtac
     1141 tcgaaactct cctgcggtcg cagaagcggc tgctcaccca cgacgcccgc gccgcgcact
     1201 tcctgcacct ggttatccgc atcggtgatg atccagtgcc ggctgaggag ctgggccgcg
     1261 atgtttcctg tgttcgtgat cgtgatggta taggcgaaga catagcgctt gcgatccggg
     1321 tcggattgct cgggaatgaa tgcggagcgc gcgctgacgc ggatgtcgtg ctttttgtcg
     1381 ggcatgcacg aatttcaccc gattggacgg cgagtggcaa gcgcttggct ggtgccgcag
     1441 cgcgcataac gggtagaatg ccgcgtcgcg cgggctcttt tccggcatgc cttaccgtat
     1501 cgcaccgagc attctttcag cagactttgc gcgcctcgga gaggaggtgc gcggggtcgt
     1561 cgcggcgggc gctgacatcg tgcatttcga tgtgatggac aaccactacg tgcccaacct
     1621 cacggtggga ccgcttgtct gcgcggcgat ccgaccgatg atccaggttc ccatcgacgt
     1681 gcacctcatg gtgaagccgg tcgaccgcat cgttccggat ttcgccgagg ccggcgccaa
     1741 tatcatctcg tttcacccgg aagcgaccga ccacgtggat cgcacaattg gcctcatcaa
     1801 gagctgcggt tgcaaggccg gacttgtctt gaatccggca acacccctca cttggctcga
     1861 ccatgtgatc ggggagctcg atctggtcct catcatgtcc gtaaatccgg gcttcccagg
     1921 ccaggccttc attcccgaag cgctcaacaa gctgagcgcg gcacgtcgcc gcatcgagga
     1981 gagcggccgc gagatctggc tggaggtcga tggcggtgtc aagatcgaca acgtgcgcga
     2041 gatcgcgcag gcgggcgccg atacgttcgt tgcgggatcc gcgatatttg gcactcagga
     2101 ctaccgggcc acgatcgcgg caatgcgcgc cgagctcaag cgggcctgaa tggaaatcac
     2161 tgcacgcatg caccgggcgg agtcccgttt tccgctgcgc gtcaaggcgg tattgatcga
     2221 tctcgatggc acgctgctcg ataccattcc cgaccttgcc gttgccgcga atctgatgct
     2281 cgaagccctc ggcgagccgc cgctcgacgc ggaggttatc cgcacgttcg tgggcaaagg
     2341 catccccaat ctcgtcaagc gcacgcttgc ccgcgacctc gacggcgagg tcgaggagag
     2401 ccgtttcgag cgcgcgcttc cggtctacga gcgctgctat gcgagcgtaa acggccggca
     2461 taccacaatc tatcccggcg tgacagacgg gctcgaacgc ctgagacaag ccggctttcc
     2521 gctcggctgc gtcaccaaca aagcgatgcg cttcacgcgt ccgctgctcg agcagacggg
     2581 tctcgcgcgc tatttttccg aggtggtcgc cggtgacacc ctgcccagga aaaagcccga
     2641 tcccgagccg ctcctctacg catgcgagag attcggcgtt tcacctgcgg acatgctcat
     2701 gatcggcgat tcgctcaacg atgcgcaggc cgcgcgcgcc gccggctgcc cggtgttctg
     2761 cgtcacctac ggctacaacg agggccgtga cgtgcgggag ctcgacgtgg atgctatagt
     2821 ggcgaggctc gacgaagccg tgcgactcat cgaaaaagtc tgatcctcat gcgctccacg
     2881 ggttgcgaaa ccggaagaac agcgcgctgc gccagccagc actggcgtcc ctggcgcccg
     2941 tagtgctttc cggcgtgcgc tgcacgcagc attgcccgat ttccgctgca cccctggagt
     3001 tcgccatgac tgaagatgaa tttgcccgtc tcgcctcggc aggctacaac cgcgtgccgc
     3061 tcgtgctcga gacgtttgct gacctcgata cgcccctgtc gatttatctc aagctcgcca
     3121 accagccgta cagctatctg ctggagtccg ttgtcggcgg tgagcgcttc gggcgttatt
     3181 cgttcatcgg actcgcggcg gatgcgcgta tcgaagtgca tgggcgtgcg tgccgggaat
     3241 accgcggtgg ccgcgtcgtt tcggaggcga cgagcgagga cccgcttgct tatgtcgatg
     3301 cgtatctcgc gcgcttcaat gtcgcgcagg acgcgcgcct tccgcgcttt tgcggagggc
     3361 tcgtcggtta ctttggctat gacactgtgc gctacatcga gaagcggctc gcatcggtcg
     3421 acaaaccgga tcccctcggc acgccggata tcctgcttct ggtctcggaa gaaatcgcga
     3481 tcgtggacaa tctttccggc aagctctcgc ttgtcgtcta tgccgaaccg ggcgcgcctg
     3541 gcgcatatgc gaaggcgcga gggcggctgc gcgagttgct cggaaagctg cgcgagccgg
     3601 tcccgattcc gccagacgtt tcgacgacat cggagcctgc agtttcgggt ttcggcgagc
     3661 ggccctatca cgccgcggtc gaaagggcca agcgctacat ctacgatggc gacatcatgc
     3721 aggtcgtgcc ctcgcagcgc atgtccaaga gattccgggc gacgccgatg gcgctctacc
     3781 gggcgctgcg cacgctcaac ccctctccct acatgtttta tttcgacttc gaggactttc
     3841 acgtcgtcgg cgcgtcaccg gaaatcctcg tgcgcctcga aggcgatacc gtcaccgttc
     3901 gtccgatcgc cggtacgcga ccgcgcggcg caactgccgc cgaagacgca gcgctctcgg
     3961 aagagctgct gtccgacgca aaggagcggg ccgagcacat catgctggtc gatctcggcc
     4021 gcaacgacgt gggacgcgtc gcgcaaaccg gcaccgtacg ggtgaccgag cagatggtca
     4081 tcgagcgcta ctcgcacgtc atgcatatcg tgtctaacgt cgaggccacg ctgaagccgg
     4141 gcttgcacgc gatcgacgtg ctgcgtgcaa cctttccggc gggaacggtg tcgggcgcgc
     4201 cgaaagtgcg cgccatggag atcatcgatg aactggagcc cgtgaagcgc ggtgtgtacg
     4261 ccggggccgt gggttatctc ggcttccacg gcaatatgga cgtcgccatc gcattgcgca
     4321 ctgcagtgat caaggatggc gtgctgcacg tccaggcggg cggcggcatc gtggcggact
     4381 cggagcccca cgccgaatgg caggaaacac agaacaaggc aagagcgctc ctgcgggcag
     4441 ccgaaatggc ggaatccggc ctggataccc gtctgccgtg acgggccggc gccgcacgca
     4501 gccgagcgta aactaacgca acaccatgcg cttccgggtt ctggccactg tcgtgcgaaa
     4561 cgcactcgtt cgcctcgaaa tcatggcgaa ccgtctgctg cgccgcgagt tcgttccgga
     4621 cggcccgggc atctttcaca tcgagaccag cggggcctgc aatctcaagt gccgcttctg
     4681 cgcctacgaa aaaaaatcgg cgcccaaagt cagcatgccc aatgacttgt tcttcgactg
     4741 cgtcgcgcag gcggtggcgc tcggatacac gcgcttcgac ctcacgccgt ccaccggcga
     4801 tgtcttcatg gacaagcgac tgttcgacaa attctcttat ctcgaggatc accccggtgt
     4861 ggaagagtat tcatttttta cgaatctcac cataccgaaa ccggaagcgc tcgcgcggtt
     4921 gatcgcattg cacaagctgc ggcagctcac gatcagcatc tacggccacg acctcgcgag
     4981 cttcatcgcc atcaccaaat cgaccgaaaa cgtgtaccgc cggctcctcg cgaatctcga
     5041 cgcgctgctc gcgctgaagg aacgctggcc gttcggtgtt gcgatcgggt tccgctcgac
     5101 gttcgacgtg ccgggcgaag atgcaagcga gctcatgcag gcactcgcgc gttatcgcga
     5161 tgccggtatt ccggtgaggc cttcgcacgg cgtctacaac aattggggcg gatatatttc
     5221 gcaagccgat gtcgccgggc tcgacatgca tatcacttca acagaactca cctacaagta
     5281 tggggcatgc gtgaagctct tcgactcggt gcaggtgatg gcgagcggcg tcgtgaatgc
     5341 ctgcgcctgc cgcgacgtcg atgcgaccct gcgcatcggg aatgcgcgca cgacgcggct
     5401 caaagacatc ctctcggagc gcaacgccga gtacatgcgc atcatcgatg agcagcagcg
     5461 cggcgaattc cggccggtat gtgcaagctg cgacttctac aagagcgtct accaccagcg
     5521 ctccgagtac cgccgcagtc gcgcccccat gcagacggtg gacgacttca aggccaagct
     5581 gggggcgtcc cgccagtcag attccgggca agaaaaagcc gcgccctaag gcgcggccaa
     5641 tgtgcaatat cggtatacga cggatactgc aaccacatcc cgaggaggaa aaagaggaat
     5701 gcggcaccgc acaacgtacg ccccgaagct tacacgaagc ttacggacgc gaatttgttt
     5761 ccggatgctg tgcaccacgt cacgctgaaa ggggccgttc gagccgggcg atcaaccgtt
     5821 ccatttcgag cccgcctttc tttacccgca atgcgacggc aagcaggcgc gaactcaagg
     5881 tgagccctgc ccgttccgcc ggcgcgaccg caatctcgaa ctgcacccga cgcttcaccc
     5941 tgacaaagtt gatcatggcg ccatgctcca gcccatccgg cgcatcggtg acgatcagga
     6001 tgggacgctc acgtgccgcc tcgatcaagc gcggcatgcg ggacacggca tccgcgccga
     6061 tgaagaggac ctgcacgccg ttaagctccg ctggagcatt gatcggtcgc acggtgacac
     6121 tgcggccttg tatggttctc gccgtcacga tgcgcgaaag ttcgccggca atgtcttccg
     6181 cacctgcaac gccgatcaca agcggggcgt ctgcagccaa cgaatgccgg ggccattcca
     6241 cgtagcccat gaacttgtag acgaaggccg ccttcacctc gatttcgctc gcgccatcgt
     6301 cgatttgcgc gacggcgttt cccgagacga gcagcgcacc gcccgcgatc acgcgcaccc
     6361 aaggtgcggc gcgcacgcgt ctcatgatgc ttgcgatcag tgttgccata tcattttgaa
     6421 gaatacgccg cgttcgactt caccgcgcgt ggcgggcgcg ccgaattcgg gatgccggcg
     6481 atcgaataga ttggtgagcg tgaccgaaac ttcaagatcg cgctgcacct gccatcccca
     6541 gcgcgcgtcc agcgccgtgt aggaaggcac gttcgggttc ggcagcgctc cgacacgccg
     6601 cagcgagacg tcgagcgcat ggcgcgccgt cggctcgaat gccgaacgca ggatccactg
     6661 gtgtgccggg tcgttaccca atgcggctat ccccgcgacg tcacgactat ccggcttcag
     6721 tcgcaacgcc tgtttcagca gcacgccgcc gcccgacagg cgccagcgat cgctgacgcg
     6781 gtagtgcgcc cagccctcga cgccgtaggt cgagccctcg atcctgttct cgagcacgaa
     6841 tccgccgctg ggcgccggct ccacgctgcg caagtggtcg tgatcgtgat gaaacgccgt
     6901 caccgagtac gaataccggc tggaaggctg cgcgcggtaa ccgacctcaa tcacattcga
     6961 tacttccgag cggaagttcg gcccgccgtt cagcggcagg ggcgggacga atatgaagaa
     7021 ttcgcggtcc aggcgcgccg gcgagcgcac cgcgcgggtc aaggcggtcc ataccagctg
     7081 gtctggcgcc ggcttccatg cgatgcgcgc gcccggcagc aattccgttc cggtgtaggg
     7141 attgccttcc acccgcgcgc cgagcgtgag ggcgagtgtt gcgccgaggg cgatctcgtc
     7201 ctgaacgaaa aaattccacc agtcgaggct gcgctctgct ggaaggaacg cgagcacggc
     7261 gctgttgcca acgcggtcat tggcgtagcg gtagccggcg ccccatacga cggtatgcgc
     7321 gccgcgggct ttcagcgcgt gctgcagctc gacgtcgaag gtatcgagct cctcccgaaa
     7381 aacacctggc tgatcgcgct cgctgcggtc gtagtagccc tgcacccgca agttcgaacc
     7441 gtctccaagt tgctgattcc agcgtgcaag gaggttcgcg ccctccactt tggttctgcc
     7501 cggagcctcg ccgagatcac cgtgataggc atcgccctgc agcgtgaagg ctcgatcgcg
     7561 cccgccccag tcggcgcgaa atccgaactg ccctttgtcc caggaatcgc gcagagacgt
     7621 tccgtccgcg cggcgggtgc cgtcgcggtc gaagaatttc ccgtagagcc gaaaatgacc
     7681 gtcattccgc aatggggccc cgtagcgcac cgcgcctccg cgctcccgat tcccgccgcc
     7741 gaggctcgcg agcgcacctt gcgtggcgct tgccggtcgc gtcgtcacgt tgatgacgcc
     7801 gttgaccgca ttcgttcccc aaagggtcgc acccggccca ctgatcactt cgatgcgctc
     7861 gacatcctcc agcatgacat cctgcgcatc ccagaagaca cccgagaaaa gcggtgtgta
     7921 caccgtgcgc ccgtcgatca ggacgagcag cttgttcccg acggagttgt tgaagccgcg
     7981 cgcgctgatc gcatacgagc tcgaattgac gcgtgcgact tgcaggtttg gggcgaggcg
     8041 cagtgcctcg ggaaggctcg tcgcgccgga gcggcggatg tcctcgtgag tgatgacgaa
     8101 gatcgacgcc ggcgcctccg aaagccgctc ggcgcggcgc gagaccgaag tcacttcgat
     8161 attggcgagc tcttcgagac tgagatcggc gagttcccgc gcccccgccg gcgccgcggc
     8221 cgccccgagt accagggcca acgcgagtcc cgccgaggtg gatcggcccc gtcggcacct
     8281 cgtatacaca tcctcatact gaggcctcga cgccgaaagg atcggcacgt tacaaattcc
     8341 cctttacgcg cggagtgaga ggcgagcgtg cgcattcgcg tggcgcacgc ccggatccgg
     8401 ccagccgtgc gaatgctacc agtccccatg cgggcggagc aatcgcggca caggcggctc
     8461 tcgaatgatt caggtaaaat tttcac
//
LOCUS       DASURG010000266         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1477519, whole genome shotgun sequence.
ACCESSION   DASURG010000266 DASURG010000000
VERSION     DASURG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477519"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFY80_04605"
     CDS             <1..642
                     /locus_tag="VFY80_04605"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEX6007218.1"
                     /translation="IDALFTPRLPEERHRYYHRCWNNRRWGALFRLFFSRFVMGRLGR
                     DPRFFRYVEGAVASRILARAEHGLVDLDPSSNPYLQWIVYGRFKTQLPFLWREENFDR
                     IRRNVDRLELRVASVEASLERAPDASFDRFNLSDIFEYISEGASEAVFDNVARCARPG
                     ARIAYWNMMAPRARPERLASQLYTLHAESQRLHRKAQTFFYGAFRVDEARQTS"
     gene            666..2006
                     /locus_tag="VFY80_04610"
     CDS             666..2006
                     /locus_tag="VFY80_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007219.1"
                     /translation="MLMLLVMTGALLAGLRYYQVRLKPDPELVRKLFHLGGGLLGLSL
                     PWLFDDLLPVLVLTALVASGFVATRTIPALRNGIGQVLLGVQRPSVGEFGFLASFCLL
                     FWLARGDKLLYSIPIMMVALADALAALVGHAYGKLPLHMSGDRKSVEGVVTFFLIAFF
                     CVHVPVLLWGGTGRLESLLIAVNVSGMVMLAEAAAWWGLDNLIIPLWGYMVLKSLLQA
                     QSVSLASDLAFLLALGTFMFLWRSRATLGDDAISGAVLWGYVVWTVGGWPWIIPPLLQ
                     FAVYATATARTPLDSVRNLRFPVVLANVAGSIPWLLAYRETSDAAFYLPFATCFGANV
                     ALIALARAKFAAPGLALLPATKHGATTGMIIVLPSMLAVYGVRAMSLIAIVAAFIAVS
                     AAAAVFFRTQPRLTENPVGYDRWVRQAIIVALGSVLAFGIGGTLIRTLDSLMAQ"
BASE COUNT          303 a          659 c          601 g          448 t
ORIGIN      
        1 atcgacgcgc ttttcacgcc gcgcctgccg gaagagcgac atcgctacta ccaccggtgc
       61 tggaacaacc ggcgttgggg tgcgctcttt cgcctcttct tctcgcgctt cgtcatgggc
      121 aggctggggc gcgatccgcg cttcttccgc tacgtggaag gcgccgttgc ttcccgcata
      181 ctcgcgcgcg ctgagcacgg cctggtcgat ctcgatccgt cgtccaatcc ctacctgcag
      241 tggatcgtgt acggacgatt caagacgcag cttcctttcc tgtggcggga ggagaacttc
      301 gatcgcatcc gccgcaacgt ggatcggctc gaattgcgcg tcgcatcggt cgaagcaagc
      361 ctcgagcgcg ctccggacgc cagtttcgac cggttcaatc tctccgacat attcgaatac
      421 atctccgaag gcgcgagtga ggcggtgttc gacaacgtcg cgcgctgtgc acgacccggt
      481 gcgcggatcg cgtactggaa catgatggca ccacgcgccc gccccgagcg ccttgcttcg
      541 cagctctaca cgctgcacgc cgaaagccag agactccatc gcaaggcgca gacgttcttt
      601 tacggtgcct tccgcgtcga cgaagcgagg caaacttcgt gagccgtgca tcgacatggc
      661 taacgatgct gatgctgctc gttatgaccg gcgccctgct tgcaggcctg cgctactatc
      721 aggtcaggct caagccggat cccgagctcg tccggaagct ctttcacctc ggtggtggat
      781 tgctgggcct ttcgctgccg tggctgttcg atgatctgct tcccgtgctc gtgctgaccg
      841 cccttgtcgc gtccgggttt gtcgcaacac gaacgatacc cgcgctgagg aacggtatag
      901 gacaggttct gctcggcgtc caaagaccga gcgtcggcga attcggtttc cttgcgagct
      961 tctgcctgct gttctggctc gcccgtggcg acaagctgct ctacagcatt ccgatcatga
     1021 tggtcgcgct cgccgatgcg ctcgccgcgc tcgtgggcca tgcctatggc aagctgccgc
     1081 tccacatgag cggcgatcgc aagagtgtcg aaggcgtcgt gaccttcttc ctcatcgcct
     1141 tcttctgcgt gcacgttccc gtgctgctgt ggggcgggac cggccgcctc gaaagcctgc
     1201 tcatcgcagt caacgtgagc ggcatggtga tgctggccga ggcggctgcg tggtggggcc
     1261 tcgacaatct catcattccg ctctggggtt acatggttct caagtccctg ctgcaggcgc
     1321 aaagcgtcag tctcgcgtcc gatctcgcgt tcctgctcgc actcggcacc ttcatgtttc
     1381 tctggcgatc gcgcgccacg ctgggtgacg atgcgatttc gggtgccgtg ctgtggggtt
     1441 atgttgtatg gaccgtcggc ggttggccgt ggatcatacc tccgctgctg caattcgcgg
     1501 tttacgcaac cgctaccgcg cgcaccccgc tcgacagcgt gcgcaatctc cgctttccgg
     1561 tcgtcctcgc aaacgtggcc ggcagcattc cctggctgct cgcctatcgg gaaacgtccg
     1621 atgccgcctt ctacctgccc ttcgcgacct gcttcggcgc caacgtcgcg ctcatcgcat
     1681 tggcaagagc caaattcgcg gcgcccgggc tcgccctgtt gccggcgacc aaacacggag
     1741 cgacgaccgg catgatcatc gttcttcctt ccatgcttgc cgtatacggc gtgcgtgcca
     1801 tgagtctgat cgcgatcgtc gccgctttca ttgccgtgag tgcagccgcg gcagtgttct
     1861 ttcgcacgca accccgtctt accgaaaatc cggtcgggta cgatcgctgg gtgcggcagg
     1921 caatcatcgt ggcgctcggt tctgtcctcg cgttcggcat cggcggcacg ctcatccgga
     1981 cactcgattc gttgatggcg caatgatgtt g
//
LOCUS       DASURG010000267         5993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_353159, whole genome shotgun sequence.
ACCESSION   DASURG010000267 DASURG010000000
VERSION     DASURG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353159"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFY80_04615"
     CDS             <1..479
                     /locus_tag="VFY80_04615"
                     /inference="COORDINATES: protein motif:HMM:NF036713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6007220.1"
                     /translation="GETLQADLVLSAIGLKPKTDLAHAAGLSIRGGIIVDRTLATSDP
                     SIHALGDCAEVDGRVLPFVMPIMHAARALAATLAGKPTPVAYPAMPVLVKTPACPTIV
                     APPPPGAAGEWVVEHSADGVKSLYRDRAGKLLGFALNGAATAERAKLARELPAVLA"
     gene            536..1156
                     /locus_tag="VFY80_04620"
     CDS             536..1156
                     /locus_tag="VFY80_04620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6007221.1"
                     /translation="MFPSIPPVTWALLIANAIVYLLQGLVGDVLVLWFALWPLHGSGP
                     GGMAGFLPWQLITYSFLHGNLLHLLVNMLALYMFGSDIERVFGERRYLIYYFACVVTA
                     AIAQLIVSASSGGPPYPTVGASGGVFGVLLAFALYFPHRTVMLVFPPIPMPAWLFVTL
                     YGVLELYLGITGTQQGVAHFAHLGGMLGGYAMIRYWRSRPPVRRRW"
     gene            1221..2189
                     /locus_tag="VFY80_04625"
     CDS             1221..2189
                     /locus_tag="VFY80_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007222.1"
                     /translation="MASIARVVIRAACLACALFATAALSAEYPTRPIRYVVAFAPGGI
                     NDIMARIVAQKLNESWGQPVIVDNRPGAGGNLAAELVSKTTPDGYTFMNISTAHTIAQ
                     TLYARLNYSLVRDLTPVVVLGTAPLIMVVNPGIPAKNVSELIAWAKKNRLIYASGGAG
                     VISHLSMEMFKVAAKIEGTHVPYKGVGPAVPDLISGQAHVMINAVPELFPHAKGGKLR
                     IIATLTEKRHPFMPEVPTMIEQGYKEFVMGNWTGIVAPAKTPKPVVNKLATEITRIIR
                     SPEVSKRLLDMGVDPLGGTPEEFGKLIRFEIERFGKAVKASGARVD"
     gene            complement(2285..3424)
                     /locus_tag="VFY80_04630"
     CDS             complement(2285..3424)
                     /locus_tag="VFY80_04630"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6007223.1"
                     /translation="MFQTLRRALSFVCAMALTAPSMTAAQGKVFKSEKHAFRVVTLVE
                     GLSFPWSLAFLPDGRMLVTERDGRLRIVSKDFKLDPKPVEGVPRVVATGQGGLFDAAL
                     HPRFAENGIVYLAYNGAGSGGWGTELVRAKLEGHRLTQQQVLFRLEPKSRAGLHFGGR
                     IVFDRQGFLYLTLGDRGDKERAQELGDHAGSVIRLHDDGSVPKDNPFVTRAGAKPEKF
                     TLGNRNIQGAALHPETGELWTHEHGPQGGDEVNVMRAGRNYGWPVITYGVNYGIGTRI
                     GEGTHKPGMEQPLHKWVPSIAPSGMAFYVGDRFPGWRGNILVGALRSEMLVRLEVQGE
                     KIVREERMLKGVLGRIRDVRVGPDGLVYILTDDSDGVLARLEPVP"
     gene            3455..4654
                     /locus_tag="VFY80_04635"
     CDS             3455..4654
                     /locus_tag="VFY80_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX6007224.1"
                     /translation="MKYAMTIVIATACIAPPAFAQSEPSGSPSVRNCLEPAAWYTMDE
                     RGPRASGAHDVLADMSRRDVVLLGEQHDDAAHHRWQLQTLAALHLLRPDLVIGFESFP
                     RRMQPVLDRWVAGELTATQFLEQTEWNRIWNFPPDLYLPLFHFARLNRIPMVALNVDR
                     ALTEAVRQKGWDAVPENLKEGVSRPVPASDAYVESLFQVYRQHARDSKGQPAPADRND
                     AAFLYFVEAQLTWDRAMAEALASRRTQRDGVRPLAVGIAGSGHVRGGHGIPHQLRSLG
                     VTSIGTLLPVNARQECENMKAGLADAVFALVDAPPEAVPRPRLGVRLEQSGGEVRLAD
                     VVTGSLAEKTGLERGDRIVSIAGAPVKRMAAVIAAVRTQPAGTWLPIEVLRNREKREF
                     VIKFPRE"
     gene            4678..>5993
                     /locus_tag="VFY80_04640"
     CDS             4678..>5993
                     /locus_tag="VFY80_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX6007225.1"
                     /translation="MRRCAVRFLVAALASCTMAASAAPRAHLGLEVHLDPETRKLHAI
                     AELSATRESTFLLHPSLTIVSASANGQAVNARIVEGNEQLRRWHIAAPREAQWRIEYG
                     GTLPALDRTIDHRGVLRARGPMAAAEGSFLPAGSGWYPEPAARFSYRVRLSLPAGQRG
                     LVPGRLAAESTSGVSDRYEANFEFDAPAEGIDLMAGPYTVREKLVPREGRPPLRLRTY
                     FYGDMEALAEGYLQDSERYIALYTREIGDYPFDAFAVVASPLPTGFGMPTLTYIGREV
                     LKLPFIRGTSLGHEVLHNWWGNGVYVDYAKGNWSEGLTTFMADYFYKERESAAAAREM
                     RLAWLRDFAAVPPGAHRPLATFRSRTHGAEAVLGYGKAAMVFLMLRDEIGEAAFRDGV
                     RRFWKTHRFRTASWDDLRHAFEKASGRGLGRFFEQWIERSGGPSYRS"
BASE COUNT         1049 a         2007 c         1870 g         1067 t
ORIGIN      
        1 acggagagac actgcaagcc gacctcgtac tctcggctat cggtctgaag ccgaagaccg
       61 acctcgcgca tgctgccggg ctttccattc gcggcggcat cattgtcgac cgcacgctcg
      121 cgacgagcga ccccagcatc cacgcgctcg gcgactgcgc ggaagtggac ggcagggtgc
      181 tcccgttcgt catgccgatc atgcacgcag cccgcgcgct tgcggccaca ctcgccggga
      241 aaccgacgcc cgtggcgtat cctgcaatgc ccgtgctcgt caaaacgccc gcatgcccca
      301 ccatcgtcgc gccgccgccg ccgggcgcag ccggcgaatg ggtcgttgag cacagcgccg
      361 atggcgtcaa gtccctctat agggaccgcg ccggcaagct cctcggtttc gcgttgaacg
      421 gtgcggccac cgccgagcgg gcgaagctcg cgcgcgagct gcctgcggtc ctcgcttgaa
      481 aaaccttatc cgccgctgcg ctgtcgaagg gcagacctga caacgcgagc gccgaatgtt
      541 cccttctatt ccgcccgtca cctgggctct cctcatcgcc aacgccattg tctatctgct
      601 gcagggcctg gtcggcgacg tgctggtcct ctggttcgcg ctgtggccac tgcacggctc
      661 ggggcccggc gggatggcgg gctttctgcc gtggcagctt atcacctaca gctttctgca
      721 cggaaacctg ctacatctcc tcgtcaacat gctggcgctc tacatgttcg ggtccgacat
      781 cgaacgcgtg ttcggcgagc gacgctatct gatctactac ttcgcgtgcg tagtgaccgc
      841 cgccatcgcg cagctcatcg tctccgcttc gagcggcggg ccgccttatc ccaccgtggg
      901 cgcctcgggc ggcgtgttcg gcgtgctgct cgctttcgcc ctctacttcc cgcatcgcac
      961 ggtcatgctg gtgttcccgc cgattccgat gccggcgtgg ctctttgtaa cgttatacgg
     1021 cgtgctcgag ctctatctcg gcatcacggg cacgcagcag ggcgtcgcgc attttgcgca
     1081 cctgggcggc atgctggggg gatatgccat gatccgctac tggcgaagcc gcccgccggt
     1141 acgccgccgc tggtagaatc ccggagagat tccgaactcc gggtctgtcc cggcaaaaac
     1201 ataacgagag gaggtcttcc atggcatcca tcgcgcgtgt tgtgatccgt gcggcttgcc
     1261 tcgcctgcgc gctcttcgcg actgccgctc tctccgccga gtatccgacg cggccgatcc
     1321 gctatgtggt ggcgttcgcg cccggcggca tcaacgacat catggcccgc atcgttgcac
     1381 aaaagctcaa cgaatcgtgg ggccagccgg tcatcgtgga caaccgtccg ggtgccggcg
     1441 gaaacctcgc cgccgagctg gtctcgaaga cgacacccga cggctacacc ttcatgaaca
     1501 tcagtaccgc gcataccatc gcgcaaacgc tctatgcgcg tcttaactac agcctggtgc
     1561 gcgatctcac cccggtcgtc gtactcggaa cggcaccgct catcatggtg gtgaatcccg
     1621 gcatcccggc caagaatgtg agcgagctga tcgcatgggc gaagaagaac cggctgatct
     1681 acgcctccgg tggcgcggga gtgatcagcc acctgtctat ggagatgttc aaagtcgcgg
     1741 cgaaaatcga ggggacgcac gtgccctata agggcgtcgg ccccgcggtg ccggatctca
     1801 tttcgggaca ggcgcacgtc atgatcaacg ccgtgccgga actctttccg catgcgaaag
     1861 gcgggaagct gcgcatcatc gcgacgctca ccgagaagcg ccacccgttc atgcctgaag
     1921 tgccgacgat gatcgagcag ggctacaagg aattcgtgat gggcaactgg accggtatcg
     1981 tcgcgcccgc gaaaaccccg aagcccgtcg tcaacaagct cgcgacggaa atcacgcgca
     2041 tcatccgttc gcccgaagtg agcaagcggc tcctcgacat gggcgtcgat ccgctgggcg
     2101 gcacgcccga agagttcggc aagctgatcc gcttcgagat cgagcgcttc ggaaaagcgg
     2161 taaaggcgtc cggcgcacgg gtggattgat cacatcgtca ttccggcgaa agccggaatc
     2221 cagaaaatgc atgcggctta agacatgccg gagcactgtt tcaccggcat gaggggccag
     2281 cctgctaggg cacgggttcg aggcgcgcga gcacgccgtc cgaatcgtcc gtgaggatgt
     2341 aaaccagccc gtcaggcccc acgcgcacgt cgcgaatgcg cccgagcaca cctttcagca
     2401 tccgctcttc acgcacaatc ttctccccct gcacctcgag gcgcaccagc atttcgctcc
     2461 tcaacgcgcc gaccagaatg ttgccgcgcc agccgggaaa tctatcgccg acgtaaaacg
     2521 ccatgcctga aggcgcgatc gacggcaccc atttgtgcag cggctgctcc atgcccggct
     2581 tgtgtgtgcc ttcgccgatc ctggttccga tgccatagtt gacgccgtag gtgatcaccg
     2641 gccagccgta gtttcggccc gcgcgcatca cgttcacttc atcgccgccc tgcgggccgt
     2701 gctcgtgcgt ccacagctcg cccgtctcgg gatgcagcgc ggcaccctgt atgttgcgat
     2761 tgccgagcgt gaacttctcc ggcttcgcgc ccgcccgagt cacgaacgga ttgtctttcg
     2821 gcacgcttcc gtcgtcatgc agccggatca ccgagcccgc gtggtcgccg agctcctggg
     2881 cacgttcctt gtcaccgcga tccccgagcg tgagataaag aaaaccctga cgatcgaaga
     2941 cgatgcggcc gccgaagtgc aatccggcgc gcgacttggg ctccaggcgg aagagcacct
     3001 gctgctgcgt gagccggtgc ccctcgagct ttgcgcgcac gagctccgtg ccccagccgc
     3061 cgctgcccgc gccgttatac gcgaggtaca cgatcccgtt ttccgcgaac cgcggatgca
     3121 gcgcggcatc gaacaacccg ccctggccgg ttgcgaccac gcgcggaacg ccctcgaccg
     3181 gtttcggatc gagcttgaag tccttcgata cgatacgcag ccgcccatcg cgttcagtga
     3241 ccagcatgcg cccgtccggc agaaaggcaa gcgaccaggg aaaggacaat ccctctacga
     3301 gcgtcaccac gcggaacgcg tgcttttccg acttgaagac tttgccctgc gcagcggtca
     3361 tgctgggtgc ggtcagcgcc atggcgcaca cgaagctcaa tgccctgcgt agcgtctgaa
     3421 acatgaggta atctaccgcc ctatgccctg tcgaatgaaa tacgcgatga cgatcgtcat
     3481 cgcgaccgcg tgcatcgcgc cccctgcctt cgcgcagtcc gagcccagcg gcagcccatc
     3541 ggtacggaac tgcctcgaac cggccgcatg gtacaccatg gacgagcgag gaccgcgtgc
     3601 gtcaggcgcg cacgatgttc tcgccgacat gtcccgccgc gacgtcgtcc ttctgggcga
     3661 gcagcacgac gacgccgcgc atcaccgctg gcagttgcag acgcttgcgg cattgcatct
     3721 gctgcgaccc gacctcgtga tcggcttcga atcgttcccg cggcgcatgc agccggtgct
     3781 cgatcgctgg gtcgcgggcg agctcaccgc aacgcagttt ctggagcaga ccgaatggaa
     3841 caggatctgg aattttccgc ccgatctcta tctcccgctc tttcacttcg ctcgcctgaa
     3901 tcgcataccg atggtcgcgc tcaacgtcga tcgtgcgctg acggaggcgg ttagacagaa
     3961 aggctgggat gcggtgcccg agaatctgaa ggaaggggtg tcgcggccag tgccggcctc
     4021 cgacgcgtat gtggagtcgc ttttccaagt gtacaggcag cacgcgcgcg actcgaaagg
     4081 acaaccggca ccagcagatc gcaacgatgc ggcgtttctc tatttcgtcg aggcgcagct
     4141 cacctgggat cgcgcgatgg cggaggcgct cgccagccga cggacacagc gcgatggtgt
     4201 gcggcctctc gcggtaggca ttgcgggaag cggacacgtg cggggcggcc atggcatacc
     4261 gcatcagttg cgcagccttg gggtgacgag catcggcacg ctgcttcccg tcaatgcgag
     4321 gcaagagtgc gaaaacatga aagcgggcct ggccgacgcc gtattcgcgc tggtggacgc
     4381 accgcccgaa gcggtgccga ggccgcgcct cggcgttcgg ctcgagcagt ccggcggcga
     4441 agtgcgactc gcagacgtcg ttaccggcag tctcgcggag aagaccggac tcgaacgcgg
     4501 tgatcgcatc gtgtcgattg ccggcgcgcc ggtcaagcgc atggcggccg tcatcgccgc
     4561 cgtgcgcact cagccggcgg gcacatggtt gccgatcgaa gtgctgcgca accgggaaaa
     4621 gcgcgagttc gtcatcaagt ttccgcgcga atgacatccc cgccgccccg cggaacggtg
     4681 cgccgctgtg cagtccgctt tcttgtcgcg gcgctcgcct cctgcaccat ggcggcaagc
     4741 gccgcgccgc gcgctcatct cggtctcgaa gtccatctcg atccggaaac ccgcaagctg
     4801 catgcgatcg cagagctctc cgcaacccgg gaaagcacgt tcctcctgca tccctcactg
     4861 acgatagtct ctgcttcggc gaacggccag gcggtgaacg cgcgcatcgt cgaaggcaac
     4921 gaacagttgc gcagatggca tatcgccgcg ccgcgtgagg cgcaatggcg catcgagtac
     4981 ggcggcactc tgcccgcact cgatcgcacg atcgatcatc gcggcgtgct gcgcgcacgc
     5041 ggaccgatgg ctgcggcgga aggaagcttc ctgcccgcgg ggagcggctg gtatcccgag
     5101 cccgctgcgc gcttctcgta tcgcgttcga ctgagcctgc ccgcaggtca gcgcgggctc
     5161 gtccccggcc ggctcgcggc ggaatcgact tcgggcgtca gcgaccggta tgaggcgaac
     5221 ttcgagttcg acgcgcctgc ggaaggcatc gatctcatgg ccggtccgta caccgtgcgg
     5281 gaaaagctcg tgccgcgcga agggcgtccg ccgctgcggc tccgaaccta tttctatggc
     5341 gatatggaag cccttgcaga aggctatctc caggacagcg aacgctacat cgcgctctat
     5401 actcgcgaaa tcggcgacta tccgttcgat gcattcgcgg tcgtcgcgag tccgcttccc
     5461 accggtttcg gcatgccgac cttgacttac atcggccgcg aggtgctgaa gctgcctttc
     5521 atccgcggga catcgctcgg gcatgaggtg ctgcacaact ggtggggcaa cggcgtatac
     5581 gtcgattacg ccaagggcaa ctggagcgag gggctcacca ccttcatggc ggattacttc
     5641 tacaaggaac gtgaatccgc tgccgcagca cgcgaaatgc ggctcgcgtg gctgcgtgac
     5701 ttcgcagcgg tgccgcccgg cgcacatcga ccgctcgcga cgtttcgctc gcgcacgcac
     5761 ggcgcagaag cggtcctcgg ctacggcaag gcggcgatgg tgtttctcat gctgcgcgac
     5821 gagatcggcg aggccgcttt ccgcgacgga gtacgccgct tctggaagac tcaccggttc
     5881 aggaccgcgt catgggatga tcttcggcac gcgttcgaaa aggcgtcggg ccgaggactc
     5941 gggcgttttt tcgagcagtg gatcgagcgc agcggcggcc cgagttacag atc
//
LOCUS       DASURG010000268         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_761125, whole genome shotgun sequence.
ACCESSION   DASURG010000268 DASURG010000000
VERSION     DASURG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761125"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="VFY80_04645"
     CDS             complement(<1..1803)
                     /locus_tag="VFY80_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007226.1"
                     /translation="MSFVASGNGTAAPTLVTTDARGVATLTSWTLPTQAGSHQLTVST
                     AGVAPLVIEATAQPGPPAHLAIVTQPHTFNGTGQQLATQPVVEIQDQFGNRADNASGS
                     ITAVMAPGSLQTLSGTTTVMAVAGRAVFTDLAVSGIGSARLLFVSGTLTPVTSAAFNV
                     PASTLCPSTPAVLDLGPGEIRRWQMSAGDAPTCLDFPQAMVSGREYLLQFENISLRGN
                     SDTGLFPGVTTGLTFTVWVDATGTPSGNLAAPRALATPAGAVHAWDFGAGPIYEAEPP
                     EPPGGVPPVVSTRGRLRLDANLASAAAAVGDTISVFMAGIPRLGIADGVQQAIVRYVG
                     PNLIIAEDVRLGTLTRQDGTTNTPLLLADMEAIAADYAQYARVQGDLFFQNRHNSATE
                     AAQSRIIAVHSLMYADNIWGYTYPSQNYFVWDYWVGTNGSTKGVNQSIVRNANNLFMH
                     EIAHMRHYGMSERAGRTAARGNRWLVEGFARATERWPITMRLTGVTEFSRTGNIQLPL
                     FSPTSLSSLEDVPVYLAASFSVLEGYGSSSYIFDYFADQVALTGADWRVALGEFLVNA
                     GVEADLNSVIARYLPGVDVATLFTRARAALFLDDY"
     gene            2131..>2477
                     /locus_tag="VFY80_04650"
     CDS             2131..>2477
                     /locus_tag="VFY80_04650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6007227.1"
                     /translation="MLAALVAPPALVCQPAKKASGSTSNTPILDELRTKGLLIPVAGV
                     TADELYDSFRDPRSENRKHNAIDIVAPRGTPVLSADSGRILKLYRGENGGLMIYATDS
                     AERFIYTYAHLDR"
BASE COUNT          487 a          803 c          772 g          415 t
ORIGIN      
        1 atagtcgtcg agaaaaagcg cggcgcgcgc gcgggtgaac aaagtcgcga cgtcgacacc
       61 cggtaggtag cgcgcgatga cactgttgag atccgcttcc acaccggcat tgaccaggaa
      121 ctcacccagc gcgacccgcc agtcggcccc ggtcagcgcc acctggtcgg cgaagtaatc
      181 gaagatgtag gacgacgagc cgtacccctc caacaccgaa aacgacgcgg ccaggtacac
      241 gggcacgtct tccagcgagc tcaacgacgt gggactgaac agcggcagct gaatgttccc
      301 ggtgcgcgag aactccgtaa ccccggtcag acgcatcgtg atcggccagc gctccgtggc
      361 gcgcgcgaag ccttcaacca gccagcgatt gccacgggcc gcggttcgac ccgcacgctc
      421 actcatgccg tagtgccgca tatgcgcaat ttcgtgcatg aagaggttat tggcgttgcg
      481 cacgatcgac tggttcacac ctttggtcga accattcgtg cccacccagt aatcccacac
      541 gaaataattc tgcgatggat aggtgtaccc ccagatgttg tcggcataca tcagcgaatg
      601 aaccgcaatg atcctgctct gggcggcctc ggtcgcggaa ttgtggcgat tctgaaagaa
      661 aagatcgccc tgtacgcggg catactgtgc atagtccgcc gcgatcgctt ccatatccgc
      721 gagcagcaac ggcgtgttgg tggtgccatc ctgccgggtc agtgtgccca ggcgcacatc
      781 ctcggcaatg atcagattcg ggccgacata acgcacaatc gcctgctgca caccatccgc
      841 aatgcccagg cgcggaattc cggccatgaa tacggaaatg gtgtcgccca cggcagcggc
      901 tgcactcgcc aggttcgcat caagacgaag ccggccacgc gtggacacga ctggcggcac
      961 gccgcccggc ggctcgggtg gctcggcttc gtaaatcgga ccggcaccaa aatcccacgc
     1021 gtgcaccgcg ccggccggcg tggccagcgc gcgcggcgcg gcgaggttgc cgctcggtgt
     1081 gccggtggca tcgacccaaa cagtgaaggt cagcccggtg gtcacgcccg gaaacaggcc
     1141 cgtgtccgaa ttgccgcgca gggagatgtt ctcgaactgc agcaagtact cgcgcccgct
     1201 caccattgcc tgcggaaaat caagacacgt gggcgcatcc ccggccgaca tctgccagcg
     1261 ccgaatttct cccggcccga gatcgaggac cgccggcgtg ctcggacaca gcgtgctggc
     1321 tggaacgttg aacgccgcgg atgtcaccgg cgtcagtgtg ccggacacga acagcaggcg
     1381 cgcgctgcca atacccgaaa ccgcgagatc ggtaaagact gcacgccctg caaccgccat
     1441 taccgtggtg gtgccggaca gcgtctgcag gctccccggc gccatgaccg ccgtgatcga
     1501 gccactggca ttatccgcgc gattcccaaa ttgatcctga atttcgacca ccggctgggt
     1561 cgccagctgc tggcccgtgc cgttgaacgt gtgcggctgc gtgacaatgg cgaggtgcgc
     1621 cggtggcccc ggctgcgccg tcgcctcgat gacgagcggc gcgacgccgg cggtgctgac
     1681 cgtgagctga tgtgaccccg cctgtgtggg cagcgtccat gacgtaagcg tcgcgacgcc
     1741 ccgcgcgtcc gtcgtcacca gggtgggcgc ggccgtcccg ttgccactgg ccacgaagct
     1801 cacggatacg ccggacatgg ctttgttgcg cgtactcagg agtttgacgg caggatggac
     1861 cgctactggt gtaccagcgg ccgcggtttg accggcgccg gccacgactt gtagcgttcc
     1921 ggggacggaa ggctcggtgc cgtcgctaca ggcggtgagc gcaaggatca gccccgcaat
     1981 gggcgcaaag gaacggacaa acggtcggct catgcctttg aatcggcgca ggctcctaaa
     2041 agccggtgcc gggctgcaaa agggggtggc cgcaaaatct aacagcagag tagtggaccg
     2101 aacatgacca gaactttaac cagctgtctg atgttggccg cgctggtcgc tcctccggca
     2161 ctcgtttgcc aacccgcgaa aaaggcttcg ggcagtactt ccaatactcc gatcctggat
     2221 gaactgcgca ccaaggggct cctcatcccg gtggcgggcg taacggccga cgagctgtac
     2281 gacagttttc gcgatccgcg atccgagaat cggaagcaca acgccatcga catcgtggca
     2341 ccgcgcggca cgccggtact gtccgccgat tcgggtcgca ttctcaagct gtaccgcggt
     2401 gaaaacggcg gcctcatgat ttacgccacc gattctgcgg agcgcttcat ctatacctat
     2461 gcgcatctcg atcgcta
//
LOCUS       DASURG010000269         5675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1170576, whole genome shotgun sequence.
ACCESSION   DASURG010000269 DASURG010000000
VERSION     DASURG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170576"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFY80_04655"
     CDS             complement(<1..253)
                     /locus_tag="VFY80_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX6007228.1"
                     /translation="MYKILVVGPSWIGDTVLAQPLLRLLRERHDNTAAIDVLAPEWTL
                     PLLQRMPEVRRAIPNPFGHGELKLGARRRFGRALAREGYE"
     gene            complement(266..481)
                     /locus_tag="VFY80_04660"
     CDS             complement(266..481)
                     /locus_tag="VFY80_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX6007229.1"
                     /translation="MRGYEMPDQNENRERLIEVTAADLPLHCPTPSMVLWNAHPRVFL
                     PIHKTGEALCPYCGTRYVLKGEAAAGH"
     gene            complement(481..1407)
                     /locus_tag="VFY80_04665"
     CDS             complement(481..1407)
                     /locus_tag="VFY80_04665"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX6007230.1"
                     /translation="MSMADRDGQIWYDGRLVPWRDATTHVLTHSLHYGLAVFEGLRAY
                     HTARGTAIFRLKEHTERLFNSAHIYRMKIPYSREALMAAQKEVVRANRLESCYIRPIA
                     FYGSEKMGVSPTGASVHVAIAAWPWGAYLGPEAIEKGIRVKTSSFVRHHVNVSMCRAK
                     YSGTYANSILANLEATEQGYDEALLLDVDGFVAEGSGENLFMVKNGEIFEPELTSALI
                     GITRQSVITLAREMGYAVHARRITRDDLYIADEAFFTGTAAEVTPIREVDSRVIGEGR
                     RGEVTTRLQKAFFDCVTGKSERHSDWLTPVAA"
     gene            complement(1437..4124)
                     /gene="glnE"
                     /locus_tag="VFY80_04670"
     CDS             complement(1437..4124)
                     /gene="glnE"
                     /locus_tag="VFY80_04670"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX6007231.1"
                     /translation="MNNAAQVSCADITLARACAYSRFARRLIEADGTLAHLTCARAFA
                     AAEMQAALAGVPFGDETALRRALRALRKRVILCVMARDLSGAADLAEVMDTTTALAEG
                     AIDFALRRLDAELGAQHGYPMGSESRRRQQLHVIGMGKLGGRELNVSSDIDLIFVYPE
                     EGETAGSRPISNHEYFTGLGRRLIGALNEATEDGYVFRVDMRLRPYGDGGPLVMSFEM
                     LENYLITQGREWERYAWIKARALTGDRGADLMELVRPFVYRRHLDYSAFASMRDLHRQ
                     IRLEVERRELFDNIKLGPGGIREIEFIAQVFQLIRGGREPDLRRQPTLEVLGLVAERN
                     LLPSGAVQELADAYAFLRRLEHRLQYLDDQQTHTLPRNEGDRLAVAQSMGFTDYARFQ
                     AALERCREHVTRQFQQIFTATPQDRHALVSVWQSADAEGSTARLSALGYRRTGEVQKR
                     LAAFRESPRYRQMPAASQQRLERLVPLAIEAAAAVTDPDTALERVLELVESVSRRESY
                     LALLEEYPLALARLAELVSASGWVAQYLTRHPILLDELLDTRTLYAAPDWSALRRALR
                     AQLDDAANDIERQMDILCHFKHAQTMRFIAQDLGGTLPLETLSDHLSDLACVILEEVL
                     RLTWSALRTRHRDAPRFAIVAYGKLGGKELGYVSDLDIVFLYDDDAPEAPENYARLAQ
                     RINAWLTAITPAGVLYETDLRLRPDGASGLLVSPFAGFREYQEKHAWGWEHQALTRAR
                     FAAGDASMGREFEALRISILRKPRDLQALRGDIVTMRDKIHAAHPNRSTMFDLKHDRG
                     GIIDVEFIVQYLVLGYAHAHAELTGNIGNLALLKLAGRLGLIAEVQALAVHDAYREFR
                     RRQHRLRLAGEKYARVAREEMAGHIAAVLDLWRAVFGAS"
     gene            4199..>5675
                     /locus_tag="VFY80_04675"
     CDS             4199..>5675
                     /locus_tag="VFY80_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007232.1"
                     /translation="MPAALTRWLRFSSLTVYRLVTWSLLAAAFAVGAVILGLRYWVLP
                     NVDQHRETIARMIGQGIGQRIAIGRVSGNWDGLHPELVMEGITIFDREGRPALQLARI
                     EGVLSWLSFAALEPRFRSVEISEPVLSVRRDARGALHVAGIELKQGTGGGLGDWLLRQ
                     RRLSIRGAAITWQDELRGAAPLALDAVDFLLRNRGSRHRYALRAVPPRALAGPLDVRG
                     EFTGRSLSEPAEWHGRLFVQVDGVDLDGWRPWLPSSFIAPKGMGALRMWLSFREQRLT
                     ELTADVRLAKVEVRLAPELPELKFSALSGRVGWKDVPGGVEISTTRLALASTDGLVVQ
                     PTDFFLRLTAMSDSVASRGELRANALALEPLAALADHLPLERELRSDIIALSPRGNLF
                     DVALHWNGPWRHPAQYVVRGRFEALGLAGVGKLPGFAGLTGAFEGNESYGTVRLDSTD
                     VTLDMPSVFRGPLELERLVADGAWSRAGRALDLQFSKVGFSN"
BASE COUNT          930 a         1800 c         1895 g         1050 t
ORIGIN      
        1 gctcgtatcc ttcgcgcgca agcgcgcgtc cgaatctgcg gcgggcgccg agcttcagtt
       61 caccgtgtcc gaagggattc ggaatcgccc gcctcacctc cggcatgcgt tggaggagcg
      121 gcagcgtcca ttccggcgcg aggacgtcga ttgcagccgt gttgtcgtgt ctttcgcgga
      181 gcagccgcaa gagcggctgc gcgaggaccg tgtcgccgat ccaggacggg ccgacgacga
      241 ggatcttgta caccgcgcgc gtcggtcagt ggccggccgc ggcctcgccc ttcagcacgt
      301 atcgcgtgcc gcagtacggg cacagcgctt cgccggtctt gtggatcggg agaaagacgc
      361 gtggatgcgc attccacagc accatggaag gtgttgggca gtgcaacggc aagtcggccg
      421 ccgtgacttc gatcagccgc tcgcgatttt cgttctggtc gggcatttcg tagccgcgca
      481 tcaggctgca accggagtca gccagtcgct gtgccgttcc gacttgccgg ttacacaatc
      541 gaagaaagcc ttctgcagcc gggtagtgac ttcaccccgc cggccctcgc cgatgacgcg
      601 actgtccacc tcgcggatcg gcgtcacctc cgcagcggtg ccggtaaaga acgcttcgtc
      661 ggcgatgtag agatcgtcgc gtgtgatccg tcgtgcgtgc accgcataac ccatttcccg
      721 cgcgagagtg atcaccgatt ggcgggtgat gccgatcagc gcgctcgtga gctcgggctc
      781 gaaaatttcg ccgttcttca ccatgaagag attttcgccc gaaccctctg cgacgaaacc
      841 atcgacgtcg agcagcagcg cttcatcgta gccctgttcc gtcgcttcca gattggcgag
      901 gatcgagttg gcgtaggtgc cggaatactt cgcacggcac atcgagacgt tcacgtgatg
      961 gcggacgaat gaggaggttt ttacccgtat ccctttttcg atcgcctcgg gacccagata
     1021 ggcgccccac ggccaggccg cgatcgcgac atgcacgctc gcgccggtcg gcgatacgcc
     1081 catcttttcc gagccgtaga acgcaatcgg ccggatgtag cacgattcca ggcggttcgc
     1141 gcgcaccact tccttctgcg ccgccatcag cgcctcgcga ctgtacggaa tcttcatgcg
     1201 gtagatatgc gcggaattga agagccgctc ggtgtgctcc ttcagtcgga agatcgcagt
     1261 gccgcgcgca gtgtggtagg cgcgcaggcc ctcgaatacg gcgagcccgt agtgcaatga
     1321 atgcgtgagc acgtgagtcg tggcgtcgcg ccacggcacg agcctgccgt cgtaccagat
     1381 ctggccgtca cggtcggcca ttgacatgtg catgctccag aggcgaaagc cgcattttag
     1441 cttgctccga acacggcgcg ccagagatcg agcaccgcgg caatgtggcc ggccatctct
     1501 tcgcgcgcga cgcgcgcata tttttcgccg gcgaggcgca ggcggtgctg ccggcggcga
     1561 aactcgcgat aggcatcgtg cacggcgagc gcctgtacct cggcgatgag tccgaggcgg
     1621 cccgcgagct tgagtagcgc gagattgccg atattgcccg tgagctccgc gtgcgcatgc
     1681 gcgtagccga gcaccagata ctgcacgatg aactcgacat cgatgatgcc gccgcggtcg
     1741 tgcttgagat cgaacatcgt gctgcggttg ggatgcgcgg catggatttt gtcgcgcatc
     1801 gtcacgatgt cgccgcgcag cgcctgcaga tcacggggct tgcgcaggat ggagatgcgc
     1861 agcgcctcga attcgcgccc catggaggcg tcgcctgccg cgaaacgggc acgcgtgagc
     1921 gcctggtgct cccatcccca ggcgtgcttt tcctgatact cgcggaagcc cgcgaacggg
     1981 ctgacgagca gtccgctcgc gccatcgggg cgcaagcgca ggtcagtttc gtacagcacg
     2041 cctgcgggcg tgatggcggt gagccaggcg ttgatgcgct gcgcgagccg cgcgtagttc
     2101 tccggtgcct ccggcgcatc gtcgtcgtaa agaaacacga tgtccagatc ggagacatag
     2161 ccgagttcct tgccccccaa cttgccgtag gcgacgatcg cgaaccgcgg cgcatcgcgg
     2221 tggcgcgtgc gaagcgccga ccaggtgagc cgcagcactt cctcgagaat cacgcaggcg
     2281 agatccgaga ggtgatcgct cagcgtttcg agcggcagcg tgccgccgag gtcctgtgca
     2341 atgaagcgca tcgtctgcgc gtgcttgaag tggcacagga tgtccatctg gcgctcgata
     2401 tcgtttgcgg cgtcgtcgag ttgtgcgcgc agggcgcgtc tgagcgctga ccagtccggc
     2461 gccgcataga gcgtgcgggt gtcgagcagc tcatcgagca ggatgggatg ccgggtcagg
     2521 tactgtgcga cccagccgct cgcgctcacg agttcggcga gccgcgcgag tgcgagcgga
     2581 tattcttcga ggagcgcgag atacgactcc ctgcggctca cgctttccac cagctcgaga
     2641 acccgttcga gcgccgtatc gggatcggtt accgctgccg ccgcttcgat ggcgagcggc
     2701 accaggcgct cgagccgctg ctggctcgcg gccggcatct ggcgataccg aggactttcg
     2761 cgaaatgcgg cgaggcgctt ttgcacctca cccgtgcggc ggtagccgag cgcgctcaag
     2821 cgggcggtgc tgccttcggc atccgcggat tgccacacgg agacgagcgc atgccggtcc
     2881 tgcggcgtcg cggtgaagat ctgctggaac tggcgcgtaa cgtgctcgcg gcagcgctcg
     2941 agcgcggcct ggaagcgggc gtagtctgta aaccccatgc tttgcgcgac cgcgagccga
     3001 tcgccctcgt tccggggcag cgtgtgggtc tgctgatcgt ccagatactg gaggcggtgc
     3061 tccagccgcc gcagaaaagc atacgcgtcg gcgagttcct gcactgctcc gctcggcagg
     3121 aggttgcgct cggcgacgag cccaagcact tcgagcgtcg gctggcgcct gaggtcgggc
     3181 tcgcgccctc cgcggatgag ctgaaacacc tgcgcaatga actcgatctc gcgtatgccg
     3241 cccggaccga gcttgatgtt gtcgaaaagc tcgcgccgct cgacttcgag ccggatttga
     3301 cgatggagat cgcgcatcga agcgaacgcg ctgtagtcga gatggcggcg atagacgaag
     3361 ggccgcacga gttccatcag atcggcgccc cggtctccgg tgagcgcccg ggccttgatc
     3421 cacgcatagc gctcccattc gcggccctgt gtgatgagat agttctccag catctcgaag
     3481 ctcatgacga gcgggccgcc gtcgccgtat ggcctcaacc gcatatcgac gcgaaacacg
     3541 tagccgtcct cggtcgcttc gttcaacgca ccgatcaggc gcctgccaag gcccgtgaag
     3601 tactcgtggt tgctgatcgg ccggctgcca gcggtctcac cttcctcggg atagacgaag
     3661 atgagatcga tgtccgagga gacgttgagc tcgcgcccgc cgagctttcc catgccgatc
     3721 acgtggagtt gctgcctgcg cctgctctcg ctgcccatcg ggtagccatg ctgcgcgccc
     3781 agctccgcgt cgagccggcg aagcgcaaag tcgatcgcgc cttccgcgag cgcagtagtg
     3841 gtatccatga cctcagcgag atccgccgcg cccgagaggt cgcgcgccat gacgcaaagg
     3901 atcacgcgct tgcgcagcgc acgcaacgcc cgtcgcagcg ccgtttcgtc gccgaacggc
     3961 acccctgcga gcgccgcctg catctcggcc gcagcgaaag cacgcgcgca cgtgaggtgc
     4021 gcgagcgtgc catcggcttc aatcagccgg cgcgcaaagc ggctgtaggc gcacgctcgg
     4081 gcaagcgtga tgtcggcgca cgacacctga gcagcgttat tcatttccat gcacgtattg
     4141 gcgtaaaatc cgtaaatctt tgtttgaata tcgcattttt tccaagacca ggcaaaacgt
     4201 gcccgcagcg ctcactcgat ggctacgttt cagctcactc accgtctatc gcctggtcac
     4261 ctggagcctg cttgcagctg cgttcgcggt tggcgcggtg atcctcggcc tgcgctactg
     4321 ggtactgccg aacgtcgatc agcaccggga aacgatcgcg cgcatgatcg ggcagggcat
     4381 cgggcagcgc attgcgatcg ggcgggtatc gggaaattgg gacgggctcc atcccgaact
     4441 cgtgatggag ggcatcacta tcttcgatcg cgaaggccgg ccggcactcc agcttgcgcg
     4501 tatcgaaggc gttctttcct ggctctcgtt cgcggcgctg gagccgcgct tccgttcggt
     4561 ggagatcagc gagcccgtgc tctccgtccg gcgggatgcg cggggcgcgc tgcacgtcgc
     4621 gggaatcgag cttaagcagg gcacgggcgg tggtctgggc gactggctgc tacgccagcg
     4681 caggctttcc attcgcggtg cggcgatcac ctggcaggac gaactgcggg gtgccgcgcc
     4741 gcttgcgctc gatgcggtcg atttcctcct gcgcaatcgg ggaagccgtc atcgctacgc
     4801 actgcgtgcc gtgccaccgc gggcgctcgc cggtccgctc gacgtgcgcg gcgagttcac
     4861 cgggcgttcg ctgtcggaac cggccgagtg gcacgggcgg ctcttcgtgc aggtggatgg
     4921 tgtcgatcta gacgggtggc ggccttggct gccatcgtcc ttcatcgcgc cgaagggaat
     4981 gggcgccctg cgcatgtggt tgagcttccg cgagcaacgc ctcacggagc ttacagcgga
     5041 tgtgcgtctc gcgaaggtcg aagtccgtct cgcaccggag ttgcctgaac tgaagttctc
     5101 tgcgttgagc gggcgggtcg gatggaagga tgtcccggga ggcgtggaga tctcgaccac
     5161 caggctcgcg ctcgcgagta cggacgggct ggtcgtgcag ccgacggatt tcttcctgcg
     5221 gcttactgcg atgagcgaca gcgtggcatc gcgcggtgag ctgcgcgcca acgcactggc
     5281 attggagccg ctcgccgcgc tcgcagacca tctacccctc gagagggagc tacgcagcga
     5341 tattatcgcc ttatctccac gcggcaatct gttcgatgtc gcgctgcact ggaatggccc
     5401 gtggcgccat cctgcgcaat acgtcgtgcg cgggcgtttc gaagccctgg gccttgcggg
     5461 cgtgggcaaa ctcccgggat tcgccgggct taccggggcg ttcgagggca atgaaagcta
     5521 cgggacggtg cggctcgaca gcacggatgt cacgctcgac atgccgagcg tgtttcgcgg
     5581 accgctggag ctcgagaggc tcgttgccga cggcgcctgg tcccgcgcgg gccgtgcact
     5641 ggatcttcag ttctccaagg ttgggttttc caatg
//
LOCUS       DASURG010000270         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_966842, whole genome shotgun sequence.
ACCESSION   DASURG010000270 DASURG010000000
VERSION     DASURG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966842"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFY80_04680"
     CDS             <1..434
                     /locus_tag="VFY80_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007233.1"
                     /translation="DYPWRGRFITLPRLKDWLALLGFEIVGGQMACYAPPCAQQKWLE
                     RFGFMDKTGDRWWPMGGGVYFLQAVKRVHGMRLIMPAWKDTLVPRKNLATLPKRVSEP
                     PEAAAARKKLPEPSGNNVISLRKRLAARASSHPLRTPLKTQ"
     gene            431..898
                     /gene="rnhA"
                     /locus_tag="VFY80_04685"
     CDS             431..898
                     /gene="rnhA"
                     /locus_tag="VFY80_04685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX6007234.1"
                     /translation="MSGTVEIYTDGACKGNPGAGGWGAVLRYDGTLRELYGGEPHTTN
                     NRMELTAVIRALEALKRRCRVRLHTDSQYVQQGISAWIHTWKKRGWRTADNKPVKNDD
                     LWRELDQLAARHEIHWVWVRGHAGNDGNEHADALANRGVAELLAAAGKEGERI"
     gene            950..1651
                     /gene="dnaQ"
                     /locus_tag="VFY80_04690"
     CDS             950..1651
                     /gene="dnaQ"
                     /locus_tag="VFY80_04690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX6007235.1"
                     /translation="MRQIVLDTETTGLDPALGHRIIELGGVELVNRRPTGNHFHRYLN
                     PGRDIEEGALQVHGLTAEFLRDKPRFADIAEEFLRYVEDAELIIHNAPFDIAFLNHEL
                     GLLRRPPITDCCASVVDTLRMARDVHPGKKNSLDALCERYQVDNSQRTLHGALLDAQL
                     LAEVYLAMTRGQDSLAIEVQHTHAADVAAEFSALQLDLVVLQASEDELAAHLHALAEI
                     DKASGGACLWKRLGI"
     gene            complement(1686..2786)
                     /gene="tal"
                     /locus_tag="VFY80_04695"
     CDS             complement(1686..2786)
                     /gene="tal"
                     /locus_tag="VFY80_04695"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX6007236.1"
                     /translation="MNPLLQLHALGQRVWLDNLSRTLLREGGLQRLVTEDGVAGVTSN
                     PTIFYKAMSGSRHYRDELTALKQDPNLSPEARYEALAVPDIQAACDVLRPLYDRTGGE
                     DGYVSLEVSPALAHDAEETVAAALRLRGRVQRDNVLIKVPATSAGVTAIEELIGKGVS
                     INVTLMFSLEHVNAVAGAYLRGLERWAREGGELKRVKSVASLFLSRVDTLVDQKLESV
                     AAADRALRGKAGVALAKLAYQEYKKTFTGEKFAPLARRGARAQHMLWASTGTKNPEYS
                     DVLYVEPLIGPETVNTMPDATLAAFRDHGRAALTLEQDVDVARRHFETLGRLGIDMRE
                     VGETLQGEGVKLFVQSFDELLALMAQPATATA"
     gene            2918..>3243
                     /locus_tag="VFY80_04700"
     CDS             2918..>3243
                     /locus_tag="VFY80_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HEX6007237.1"
                     /translation="MPAALQSAMGLAALFLIAWLMSERRGAVPWRIVVSGVLLQLVLA
                     GALVLAPGAHHAFLALNDMLLGLERATQAGTAFVFGFLGGAPLPYAETGAGSSFVLAF
                     RALPLVL"
BASE COUNT          551 a         1055 c         1075 g          562 t
ORIGIN      
        1 aggattatcc gtggcgcggc cggttcatca cgctgccgcg tctgaaagac tggcttgcgc
       61 tgctgggatt cgaaatcgtc ggtgggcaga tggcgtgcta cgcaccgcct tgcgcgcagc
      121 aaaaatggct ggaacgcttc ggcttcatgg acaagacggg ggaccgctgg tggccgatgg
      181 gcgggggggt gtatttcctc caggcggtga agcgggtgca cgggatgcgc ctcatcatgc
      241 cggcgtggaa ggatacgctc gtgcccagaa aaaacctggc cacgctgccc aagcgggtga
      301 gcgagccgcc ggaagcggcc gctgcccgca aaaaacttcc cgagccgtca ggcaacaacg
      361 tcatcagtct gcgcaaacgc cttgcggccc gtgcgtcgtc gcacccgctg cgcaccccgc
      421 tcaagacgca atgagcggca cggtggagat ctacaccgac ggcgcatgca agggcaaccc
      481 cggcgctggc gggtggggcg ccgtgctgcg ctatgacggc acgctgcgcg aactttacgg
      541 cggtgagccg cacaccacca acaaccggat ggaactgacc gcggtcatcc gcgctctgga
      601 ggcgctgaaa cggcgatgcc gggtccggct gcacaccgat tcacagtacg tgcagcaggg
      661 catcagcgcg tggatacaca cctggaagaa gcgcggctgg cgcaccgccg acaacaagcc
      721 ggtcaagaac gacgatctct ggcgcgagct cgatcaactc gccgcacgcc acgagataca
      781 ctgggtgtgg gtgcgcggcc atgccggcaa cgatggcaac gagcacgccg acgcgctcgc
      841 gaaccgcggc gtggccgagc tgctcgccgc tgcgggaaaa gaaggcgaaa gaatttaggc
      901 gttgttagtt tgcgcggggc gcaagtcccc gctgcgggag gcaagaagaa tgagacagat
      961 cgtgctcgat accgagacga cggggctcga cccggcgctc ggccatcgca tcatcgaact
     1021 ggggggtgtg gaactcgtga accggcgccc gaccggcaac cacttccatc gctatctcaa
     1081 tcccggccgc gacatagaag agggcgcgct ccaggtccac ggcctcacgg cggagtttct
     1141 gcgcgacaag cccagattcg ccgatatcgc ggaggaattt ctgcggtatg tggaggacgc
     1201 cgaactcatc atccacaacg cgcctttcga catcgcgttc ctgaatcacg agctgggact
     1261 gctgcgccgg ccgccgatca ccgactgctg cgcttcggtc gtcgataccc tgcgcatggc
     1321 ccgggacgtg catcccggca agaagaacag cctcgatgcg ctgtgcgagc gctaccaggt
     1381 ggacaactcg cagcgcaccc tgcacggcgc gctcctcgat gcgcaattgc tcgcggaggt
     1441 ttatctcgcg atgacgcgcg gccaggatag ccttgcgatc gaggtgcagc acacgcacgc
     1501 cgcggatgtg gcggccgagt tctcggcgct gcagctcgat ctcgtcgtgc tgcaggcgag
     1561 cgaggacgag cttgctgcgc accttcacgc gctcgcggaa atcgacaagg cgagcggcgg
     1621 cgcgtgcctc tggaagcggc tggggatcta ggaacctgga gggcagcccc gcggacgcgg
     1681 gcgactcacg cggtcgcggt cgccggctgg gccatcagcg cgagcagctc gtcgaacgat
     1741 tgcacgaaga gcttcacacc ttcgccctgc agcgtctcgc cgacttcgcg catgtcgatc
     1801 ccgaggcgcc ccagcgtctc gaagtgccgc cgcgcgacat ccacatcctg ttccagcgtg
     1861 agcgcggcgc gcccgtgatc gcgaaatgcg gcgagcgtgg catcgggcat ggtgttgacg
     1921 gtttcgggcc cgatcagcgg ctcgacgtac agcacgtcgc tgtactccgg attcttggtg
     1981 ccggtgctcg cccacagcat gtgctgcgcg cgtgcgcccc ggcgggcgag tggcgcaaac
     2041 ttttcgccgg tgaacgtttt cttgtattcc tggtaggcga gcttggcgag cgcgaccccg
     2101 gctttgcccc gcagcgctct gtcggctgcg gcaaccgatt cgagtttctg gtccaccagc
     2161 gtatccaccc ggctcaggaa aaggctcgcg acagacttca cgcgtttaag ctcgccccct
     2221 tcgcgtgccc accgttccag gccacgaagg tatgcgccgg ctaccgcgtt cacgtgttcg
     2281 agcgagaaca tcagcgtgac gttgatcgaa acccctttgc cgatcagttc ttcgatcgcc
     2341 gtgacgcctg cggaggtcgc cggtaccttg atcagcacgt tatcccgctg cacccgcccg
     2401 cgcaggcgca acgccgccgc caccgtttct tcggcatcgt gcgcgagggc cggcgagacc
     2461 tccaggctga cgtagccgtc ctcaccgccg gtcctgtcat aaagcgggcg cagcacgtcg
     2521 caggcggcct ggatgtccgg cacggcgagc gcttcgtacc gcgcctcggg gctgagattc
     2581 ggatcctgct tgagcgccgt cagctcgtcg cggtaatggc ggctgccgct catggccttg
     2641 tagaagatgg tcggattcga tgtgacgccc gcgacgccgt cttcggtcac cagtcgctgc
     2701 aatccgcctt cacgcagcag cgtgcgcgaa agattgtcga gccagacgcg ctggcccagc
     2761 gcgtgcagtt ggagcagggg attcattgtc gataacctcc agctccgagc gtaatcaacg
     2821 cggagcaccg ggtcaataca agttgcggcg ggttgggctg cttgtttgga acacggtccg
     2881 cggttaacgt aacggtccga tcaaggggag atcacatttg cccgctgcgc tgcagagcgc
     2941 catggggctc gccgcgctct ttctcatcgc gtggctgatg agcgaacggc ggggcgccgt
     3001 gccatggcgc atcgtagtct ccggtgtgct gctgcagctc gtgctcgccg gtgcgctcgt
     3061 gcttgcgccc ggcgcccatc acgcatttct tgcgctcaac gacatgctgc tcgggctcga
     3121 gcgtgcaacg caggccggca cggcttttgt cttcggcttt ctcggcggcg cgcccctgcc
     3181 gtatgccgaa acgggcgcgg gttcgagctt cgtgctggcg ttccgcgcgt tgccgctggt
     3241 tct
//
LOCUS       DASURG010000271         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_864136, whole genome shotgun sequence.
ACCESSION   DASURG010000271 DASURG010000000
VERSION     DASURG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864136"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="nadC"
                     /locus_tag="VFY80_04705"
     CDS             <1..823
                     /gene="nadC"
                     /locus_tag="VFY80_04705"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX6007238.1"
                     /translation="AEIERNVTAALAEDIGTGDLTARLTSSSVTARATVVVRRDTVLC
                     GHAWFESCYRKVDPQVRMNWHVQEGAKASAGTAVCSLTGPARALLTGERTALNFLQLL
                     SAVASATRRYVDAVAGTRAAIVDTRKTLPGLRLAQKYAVKVGGGVNHRLGLYDGILIK
                     ENHIAAAGGVTAALDAARRIAPPGVWIQIEVENLAQLREALAAGARMILLDNMALETL
                     RAAVELTAGRAQLEASGGITLENVRAVAQTGVDRISIGSLTKDVTAADLSMRFES"
     gene            complement(832..1971)
                     /gene="thrC"
                     /locus_tag="VFY80_04710"
     CDS             complement(832..1971)
                     /gene="thrC"
                     /locus_tag="VFY80_04710"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6007239.1"
                     /translation="MAPASPSRSYRGLIDKYRTRLPVTADTPIVSLAEGATPLVELRN
                     VPRHVGKDVRIYAKFEGLNPTGSFKDRGMTMAVSKALEAGARAIICASTGNTSAAAAA
                     YAARAGMACFVLIPEGKIALGKLSQAMMHGSVVIQIRGNFDDGMRLVQDIAAKMPVNV
                     VNSINPYRLQGQKTIAFEVIEALGDAPDYHALPVGNAGNITAHWIGYSESTGHDTQAC
                     AFCDGQCRYRREPSATRRPVMLGYQAAGAAPIVHGRPVENPDTIATAIRIGNPVSWQQ
                     AVAAQRESGGWFHACTDEEILRAQKLLAEAEGVFCEPASATALAGLMKDLEAGRIREG
                     STIVCTLTGHGLKDPDTAIAQSAKPLSVEAEPGAIERIIRAHLPG"
     gene            complement(1958..3277)
                     /locus_tag="VFY80_04715"
     CDS             complement(1958..3277)
                     /locus_tag="VFY80_04715"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX6007240.1"
                     /translation="MRPINVGLLGIGTVGGGTFRVLRRNREEITRRAGCDITMKMVAD
                     KDLARARSLAGDEAVVTSDAGEVVSHPDIDIVVELIGGTTIAKELMLKAIANGKHIVT
                     ANKALLAQHGNEIFAAAQKRDVMVAFEGAVGGGIPIIKSLREGLTANRMEWIAGIING
                     TSNFILSEMRDKGASFDAVLKEAQQLGYAEADPTFDIEGVDAAHKLTIMAAIGFGIPM
                     QFKAVYIEGISKLTREDIRYAEELGYRIKLLGIAKRKAAGIELRVHPTLIPARRLIAN
                     VEGVMNAILVQGDVVGQTMYYGAGAGAEPTASAVIADLVDVARMLTSDPEHRVPHLAF
                     QPDQLSNVPILPIGEVETAYYLRLRVLDRTGVLADITRILADLDISIDAMVQKEPHAG
                     EDQVDIIMLTHVTIEKRVAAAIARIEALPVVSGKVTRIRLEELNGPR"
     gene            complement(3274..>3753)
                     /locus_tag="VFY80_04720"
     CDS             complement(3274..>3753)
                     /locus_tag="VFY80_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6007241.1"
                     /translation="MSKSYNMAGWRVGFMVGNRELVTALARMKSYHDYGTFTPIQVAS
                     IIALEGPQECVEAVRMVYQGRRDVLCSGLNGLGWEVPKPKATMYVWAPIPEHYRGLGS
                     LEFSKRLLAEAKIAVAPGVGFGHYGDDHVRFALIENEARTRQALRGIRDMFRRDGLL"
BASE COUNT          647 a         1232 c         1159 g          715 t
ORIGIN      
        1 agcagaaatc gagcgcaatg tcacggcagc gctggccgaa gacatcggca cgggggatct
       61 cacggcgcgc ctcacttctt ccagcgttac agcccgtgcc acggtcgtcg tccgccgcga
      121 caccgtgctc tgcggacacg cctggttcga gtcctgctat cgcaaggtgg acccgcaggt
      181 gcgcatgaat tggcacgttc aggaaggtgc gaaagcgtcc gccggcacgg cggtgtgctc
      241 cctcacggga ccggcgcgcg cgctcctcac cggcgagcgc acggcgctca attttttgca
      301 gctgctgtca gcggtcgcct cggcgacacg ccgctatgtc gatgccgtgg cgggaacgcg
      361 cgcggccatc gtcgatacgc gcaagacgct gcccggactg cgtctcgcgc agaaatatgc
      421 ggtcaaggtc gggggcggcg tcaaccatcg gctgggtctc tacgatggga tcctcatcaa
      481 ggagaaccac attgccgcgg ccggcggcgt cacggcggcg ctcgatgccg cgcgcaggat
      541 tgcgccgccc ggagtgtgga ttcaaatcga ggtcgaaaat ctggcccaat tgcgcgaagc
      601 gctcgccgcg ggagcgcgga tgatcctgct cgacaatatg gccctagaga cgttgcgcgc
      661 ggcggtggaa ctcacggcgg gacgcgctca gctggaggct tcaggcggga tcaccctcga
      721 aaacgtgcgc gccgtcgcgc aaacgggcgt cgatcggatt tccatcggca gtctgaccaa
      781 ggatgtaacc gcagccgatc tgtctatgcg attcgaaagc tgatccggcg ctcagccggg
      841 aagatgtgcg cggatgatcc gctcgatcgc gcccggttct gcttccacac tgagcggctt
      901 tgcgctctgc gcaatggcgg tgtccggatc tttcagccca tggcccgtca gcgtgcagac
      961 gatcgtgctt ccctcccgga tgcggcccgc ttcaagatcc ttcatcagac cggcgagcgc
     1021 cgtggcggac gccggctcgc agaacacgcc ttctgcttcg gcgaggagct tctgcgcgcg
     1081 aagtatttcc tcgtccgtgc acgcgtggaa ccagccgccc gattcgcgct gcgccgcaac
     1141 cgcctgctgc cacgacacag ggttgccgat gcgaatggcg gtcgcgatgg tgtccgggtt
     1201 ctccacgggt cttccatgca cgatcggcgc ggcgcccgcc gcctgatagc cgagcatcac
     1261 gggccgccgc gtggcggacg gctcgcgccg atagcgacat tgaccatcgc aaaaggcgca
     1321 tgcctgcgtg tcgtgacctg tcgactccga atagccgatc cagtgggcgg tgatatttcc
     1381 ggcattgccc acgggaagcg cgtggtagtc cggcgcatca cccaacgcct cgatcacctc
     1441 gaatgcgatc gtcttctggc cctgcaggcg gtaaggattg atcgagttca cgacgttgac
     1501 cggcatcttg gccgcgatgt cctgcacgag tcgcatgccg tcatcaaagt tgccgcggat
     1561 ctggatcacg acggagccgt gcatcatcgc ctgggacagc ttgccgagcg cgatcttgcc
     1621 ttccgggatc agcacgaagc aagccatacc tgcgcgcgct gcgtaggcgg ccgcagcggc
     1681 tgacgtgttc cctgtggagg cgcagatgat cgcccgtgca ccggcctcca gggccttgct
     1741 gaccgccata gtcatgccgc ggtccttgaa agacccggtg ggattgagcc cttcgaattt
     1801 ggcgtaaatc cgcacgtcct tccccacgtg acgcggcaca ttgcgcagct cgacgagggg
     1861 cgttgcgcct tcggcgagag aaacgatcgg cgtatcggcc gtgaccggca ggcgcgtgcg
     1921 gtatttatct atcaatccgc ggtaagacct gctcgggcta gcgggggcca ttcagctcct
     1981 cgagacggat gcgggtgact tttccggaaa cgacggggag cgcctcgatt cttgcaatcg
     2041 ccgcagccac acgtttttcg atcgtcacat gcgtgagcat gatgatatcc acctgatctt
     2101 cgccagcatg tggctctttc tgcaccatgg catcgattga aatgtcgagg tcggccagaa
     2161 tgcgcgtgat atcggccagc acaccggttc tgtccaagac cctcaaccgc agataatagg
     2221 cggtctccac ctctccgatg ggcaggatcg ggacgttcga cagctgatcc ggctggaacg
     2281 caaggtgcgg cacgcgatgc tcggggtcgg acgtcagcat gcgcgcaacg tccaccagat
     2341 cggcgatgac ggccgaagcc gtgggctccg cgcctgcgcc ggcgccgtaa tacatggtct
     2401 gccccaccac gtcgccctgc accagtatcg cgttcatcac gccttcgacg ttcgcgatca
     2461 ggcggcgtgc ggggatcagc gtcgggtgca cgcgcagttc gatgccggcc gccttgcgtt
     2521 tcgcaatccc gaggagcttg atgcggtagc ccagctcttc ggcataccga atatcctcgc
     2581 gcgtgagctt ggatatgcct tcgatgtaga ccgccttaaa ctgcatcggg atgccgaagc
     2641 cgatcgcggc catgatggtg agcttgtgcg cggcatccac gccttcgatg tcgaacgtgg
     2701 gatcggcttc cgcatacccc agctgctgcg cttccttgag caccgcatcg aagctcgcgc
     2761 ccttgtcgcg catttcggag aggatgaaat tgctcgtgcc gttgatgatg cctgcaatcc
     2821 actccatgcg gttggcggtg agtccctcgc gcagcgactt gatgatcggg atgccgccgc
     2881 ccacggcgcc ttcgaacgcc accatgacgt cacgcttctg ggcagccgcg aaaatctcgt
     2941 tgccgtgttg cgcgagcagc gccttgtttg ccgtgacgat gtgcttgccg ttcgcgatgg
     3001 ccttcagcat cagctccttc gcgatcgtag ttccgccgat caactcgacc acgatatcga
     3061 tatcgggatg actgacgacc tcgcccgcgt cggaggtgac gaccgcctcg tcacctgcca
     3121 gcgatcttgc gcgtgccaga tccttgtccg ccaccatttt catggtgatg tcgcagcccg
     3181 cccggcgggt aatctcctcg cggttacgac gcaggacgcg aaaggtgccg ccgcccacag
     3241 tcccgatgcc gagcagcccc acgttgatcg gtctcatagc agcccgtccc tgcgaaacat
     3301 atcgcggatg ccgcgcaaag cctgccgcgt tcgcgcttcg ttctctatga gcgcgaagcg
     3361 gacgtgatcg tcaccgtagt gcccgaagcc cactccgggc gctaccgcaa tctttgcttc
     3421 ggcaagaagc ctcttggaaa actcgagcga tccgagaccg cggtagtgct ccggaatcgg
     3481 cgcccacacg tacatcgtcg ccttcggttt gggcacttcc catcccagcc cgttgagtcc
     3541 gctgcacagc acgtcgcgcc tcccctgata gaccatgcgc acggcttcga cacactcctg
     3601 cggcccctcg agcgcgatga tcgaagcgac ctggatcggc gtgaacgtac cgtaatcgtg
     3661 gtagctcttc atgcgcgcga gcgcggtaac gagttcgcgg ttcccgacca tgaaccccac
     3721 gcgccaaccc gccatgttgt agctcttcga cat
//
LOCUS       DASURG010000272         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1171434, whole genome shotgun sequence.
ACCESSION   DASURG010000272 DASURG010000000
VERSION     DASURG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171434"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..906)
                     /locus_tag="VFY80_04725"
     CDS             complement(277..906)
                     /locus_tag="VFY80_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6007242.1"
                     /translation="MERAEFIHAPAEVPVARGTFPLILTLDTHGVPHRWITWQHACYY
                     HAKGQVAWSLGEEAFTVFGGVNRTTGERSCITSTSIIAIKGKAMAVKAFSQVPPLNNR
                     ELFHRDRQICAYCAGAFPISRLTRDHIVPFSRGGRDVWMNVVTACRTCNEKKSDRTPE
                     GAGMELIYLPYVPNRAEYLILTNRRILADQMEFLAQHVPAQSRLHQSNA"
     gene            1160..2563
                     /locus_tag="VFY80_04730"
     CDS             1160..2563
                     /locus_tag="VFY80_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6007243.1"
                     /translation="MSFASLELIPELLRAVAHEGYEHPTPIQAKAIPAILERRDIMGC
                     AQTGTGKTAGFTLPLLQLLAPQANPSPSPARHPVRALILTPTRELAAQVEDSVRTYGR
                     YLQLRSAVVYGGVDIAPQTKALHTGVEILVATPGRLLDHLQQKTVNLSRVEILVLDEA
                     DRMLDMGFLPDIRRILAALPRQRQNLLFSATFSDEIRKLAAQLLHHPTLIEVARRNAP
                     ADLVSHEVYEVPAPRKRALLAQLIRSRSISQALVFVRMKRDANRLARELPREGIVATA
                     IHSDRSQAEREEALAQFKRGLAPVLVATDIAARGLDIEQLPCVVNYELPYSPEDYVHR
                     IGRTGRAGMPGVAISLVAPDEARLLAEIERMLKHKLPRVAAPAPEHARDATRTEHRGR
                     ERSHRPRTHAREALADAVVNHNPESTFDFTKPYEPAAAGEGSTTPGAPWPGRPRHSRP
                     TAALLGGGPASHAKNKK"
BASE COUNT          506 a          860 c          793 g          489 t
ORIGIN      
        1 cgctccgccg cacgcatcac cacttgccgg ggcgtgagaa gcccgtcgga gcacgtggaa
       61 tgacaatgca ggtcgactct acgcatcgcg ccaggggcgc ggcaatgcaa tcgaagtcag
      121 cacgatatgc gccacgggaa accgagcata acagaaatga acatccctat cgcgatgagc
      181 tcacattcca gcaggaagtt tccgtacgcg tgccggacgc actgcgcttt ccggcattgg
      241 agggcacgaa cttccgccgc gctgcggccc gagctatcag gcgttgctct gatgcaaccg
      301 gctctgcgcg ggcacgtgtt gcgcgagaaa ttccatttgg tcggcgagta tgcgtcggtt
      361 cgtgaggatc aggtattccg cacggttcgg cacgtacggc aaatagatca actccattcc
      421 cgcgccctcg ggcgtacggt cgctcttctt ttcattgcac gtacggcatg ccgtcacgac
      481 gttcatccag acatcgcggc cgccgcgcga aaacggcacg atgtgatcgc gcgtgagtcg
      541 cgagatcggg aatgcgccag cgcagtacgc gcagatctgg cgatccctgt gaaaaagctc
      601 ccgattgttg agcggcggga cctggctgaa ggccttcacc gccattgcct tgcccttaat
      661 ggcgatgatg ctcgtcgagg tgatgcagga gcgttctccg gtcgtgcggt tcactccccc
      721 gaaaaccgta aacgcctcct ccccgaggct ccacgcgacc tggcccttcg catggtaata
      781 acacgcgtgt tgccacgtta tccaacgatg cggcacgcca tgggtatcga gcgtcaggat
      841 gagaggaaac gtcccgcgtg ccacggggac ctctgcgggt gcatggatga attccgctcg
      901 ttccatattc ttgtgcgctt accctgcggg tcgcagcgtc aaatccgttt tgatcatagg
      961 tgaaatttac catttttttc cgtttctttg ggcgggcggc atgaaggagc atgaccggtt
     1021 ataatgccca ttcgtggtgt cattcgcaca acttgttcat cttgcgtcct gcgggcgatg
     1081 cagttagccg tcgcgtggac atccaagcca ggcggcatgc gcatcgcccc gcgcgcctgt
     1141 acattcctag attgaagaga tgtccttcgc ttcgctcgaa ctgatcccgg aactcctgcg
     1201 cgccgtcgca cacgagggct acgagcaccc cacgccgatc caggcaaagg ccatacccgc
     1261 catcctcgaa cggcgcgaca tcatgggatg tgcgcagacc ggaaccggca agacagccgg
     1321 cttcacgctg ccgctcctgc agctgctcgc gccgcaggcc aacccgagcc cgtcgccggc
     1381 gcggcatccg gtgcgcgcgc tcatcctcac accgactcgt gagctggcag cgcaggttga
     1441 agacagtgtg cgcacgtatg gaaggtatct gcagctccgg tccgcggtgg tctatggagg
     1501 ggtggatatc gctccgcaga cgaaggcact gcacaccggg gtggagattc tcgtggcaac
     1561 cccgggaagg ctgcttgacc acctgcagca gaagaccgtc aacctgtctc gcgtcgaaat
     1621 actcgtgctc gacgaagccg atcgcatgct cgacatgggg ttccttcccg atatccggcg
     1681 catcctcgct gccctgccgc ggcagcggca gaatctgctt ttctcagcaa ccttttcgga
     1741 cgagatccgt aagctcgcag cccagcttct gcaccatccc acgctgatcg aggtggcgcg
     1801 ccgcaacgcc cctgccgatc tcgtgtcgca cgaagtctat gaggtgccag cgccgcgaaa
     1861 gcgggcgctc cttgctcagc tcatccgcag ccgcagcatt tcacaggcgc tggtattcgt
     1921 gcgcatgaaa cgcgacgcca atcgcctggc gcgcgaactt ccgcgcgaag gcatcgtcgc
     1981 gaccgcaata cacagcgacc gcagccaggc ggaacgggag gaggcgcttg cgcagttcaa
     2041 gcgcggcctc gcgccggtgc ttgtggcgac cgatatcgcc gcgcgcggcc tcgacatcga
     2101 acaacttccc tgcgtggtga attacgagct gccctattct cccgaagact atgttcatcg
     2161 catcggccgg accggtcgcg ccgggatgcc gggcgtggcg atctcgctgg tcgcaccgga
     2221 cgaggcaaga ctgcttgcag aaatcgaaag gatgctgaag cacaagttgc cgcgcgtggc
     2281 ggcgccggct cccgagcatg cacgtgatgc gacgcgtact gaacatcggg gtcgagaacg
     2341 cagccaccgc cctcgcacgc acgcccgcga ggcacttgca gacgcggtag tcaatcacaa
     2401 tcccgagtcc accttcgatt tcaccaagcc ctacgaaccg gcggctgctg gagagggaag
     2461 cacgacaccg ggcgccccgt ggccaggacg gccgcggcat tcccgcccga ccgcggcgct
     2521 cctgggtggc gggcctgcga gccacgccaa gaacaagaaa tagccgctga tacggaaagg
     2581 cccggcacag atggatgcgc tgagccttcc acaattcgtt ttcttctgct tcgtcatcgt
     2641 catctcct
//
LOCUS       DASURG010000273         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_559001, whole genome shotgun sequence.
ACCESSION   DASURG010000273 DASURG010000000
VERSION     DASURG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559001"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFY80_04735"
     CDS             complement(<1..227)
                     /locus_tag="VFY80_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007244.1"
                     /translation="MSGAALLESSLLIVDDNEDNRYTLMRRLQREGYRNLTAATDGQQ
                     ALDLLHSRSFDLVLLDVMMPGLNGYEVLERMR"
     gene            complement(224..592)
                     /locus_tag="VFY80_04740"
     CDS             complement(224..592)
                     /locus_tag="VFY80_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007245.1"
                     /translation="MKILYVEDNEDNVYVVKNRFARVGLTVVVAGDGAQGVAMAATER
                     PDLILMDLRMPVLDGWEATKRIKANPETRHIPIIALSAHAMTGDRERALEAGCDDYDT
                     KPVELPRLLEKVRALLPGRE"
     gene            complement(600..995)
                     /locus_tag="VFY80_04745"
     CDS             complement(600..995)
                     /locus_tag="VFY80_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007246.1"
                     /translation="MTRILCVEDSEDGLFMLHRRLTRAGYDVKITNNGKEGVEWAKTL
                     LPDLIVMDLNLPGLNGWEATRQLKSQPATSHIPIIVLTAHTSGKSRDEAFAAGCDDYD
                     TKPIDFSGLVKKIESLLHRGDQAAPSDKH"
     gene            complement(1032..>2097)
                     /locus_tag="VFY80_04750"
     CDS             complement(1032..>2097)
                     /locus_tag="VFY80_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007247.1"
                     /translation="ITFSVQDSGIGMTPEQCGKLFQSFTQADASTARKYGGTGLGLAI
                     SRRFCRMMGGDITVVSEAGSGSIFTIRLPAEVRAAQPIPVLRPAPVTPASGSSVNAPD
                     VLVVDDDPTVRSLTERFLVREGFSVVTADGGREGLRLARELRPMAMTLDVVMPDLDGW
                     TVLAALKGDPELADIAVILLTILDEKNRGYALGAADYMVKPVDRERLAGVLRGIIGRG
                     GGKVLVVDDDDILRRGLVQVLEREGWKAREADNGRQGLACLQESPPDVIVLDLMMPEM
                     DGFEVIEQLRHHPKWRDIPVVVVTAKDLTDEDRRRLNGGVERILQKDAPTRDEMLHEV
                     SATLARCVGQRRAARRAAGR"
BASE COUNT          345 a          720 c          624 g          408 t
ORIGIN      
        1 cgcatgcgct cgagcacttc gtagccgttg agacccggca tcatgacgtc gagcagcacg
       61 aggtcgaacg atcgtgagtg cagcaagtcg agcgcctgtt gcccgtcggt ggcggcggtc
      121 agattgcggt agccttcccg ctgcaaccgc cgcatcagcg tgtagcgatt gtcctcgttg
      181 tcgtcaacga tgaggaggct actctcgagc agcgcggccc cgctcattca cgacccggca
      241 gcaacgcacg caccttttcg agcagcctcg gcaattccac gggcttggtg tcgtagtcat
      301 cgcaacccgc ctccagcgcg cgctcccggt caccggtcat ggcgtgggcc gaaagcgcga
      361 tgatcgggat atggcgcgtc tcgggattcg ctttgatgcg tttggtcgcc tcccagccgt
      421 cgagaaccgg catcctgagg tccatgagga tgaggtccgg cctctcggtg gcggccatcg
      481 ccacaccctg cgcgccgtca ccggccacca ccacggtaag cccgacgcgc gcgaaacggt
      541 ttttcacgac gtagacgttg tcttcgttgt cctcgacgta cagtatcttc acgacagttt
      601 cagtgtttgt cagatggcgc cgcctggtcg ccccggtgca gcagcgattc gattttcttc
      661 acgaggccac tgaaatcgat cggcttcgtg tcgtagtcgt cacaacctgc tgcgaaggct
      721 tcgtcgcggc tctttccgga agtatgggcg gtcagcacga tgatcggaat gtggctggtc
      781 gccggctggc tcttgagctg ccgcgtcgcc tcccaaccgt tgagcccggg caggttcaga
      841 tccatcacga tcagatcggg cagcagcgtc ttcgcccatt cgacgccctc tttgccgttg
      901 ttcgtgatct ttacgtcgta tcccgcacgc gtcaatcttc ggtgcagcat gaaaagtccg
      961 tcctcgctgt cttcgacaca cagtattcgc gtcatgctcg cctcctttcc aggctcctct
     1021 gcgcaatacg ctcaccgccc cgccgcacgg cgcgcggcgc gccgctgccc cacgcatcgc
     1081 gcaagcgttg cgctcacttc gtgcagcatt tcatcgcgcg tcggcgcgtc cttctgcaga
     1141 atgcgctcca ccccgccgtt caggcgccgc cgatcctcgt cggtcaagtc cttcgcggtg
     1201 accaccacca ccggaatgtc acgccacttg ggatgatgac gcaattgctc gatgacttcg
     1261 aagccgtcca tttccggcat catgagatcg agcacgatca catcgggagg tgactcctgg
     1321 aggcaggcga gaccctggcg gccattgtcc gcctcccgcg ctttccagcc ttccctttcg
     1381 agaacctgta cgagccctcg ccgcagtatg tcgtcatcgt ccaccaccag caccttgccc
     1441 ccgccgcggc cgatgattcc gcgcaggaca ccggccagcc gttcgcgatc caccggtttc
     1501 accatgtagt cagcggcgcc cagcgcataa ccccggttct tttcgtcgag gatcgtgagc
     1561 aggatgacgg cgatatccgc gagctccgga tcgcccttca gcgctgcgag cacggtccag
     1621 ccgtcgagat cgggcatcac gacatcgagc gtcatcgcca tcggccgcag ctcccgtgcg
     1681 aggcgcaagc cttctcggcc gccgtccgcc gtgaccacgg aaaatccctc gcgcaccagg
     1741 aaacgctcgg tcaagctgcg cacggtcgga tcgtcgtcca ccaccagcac gtcgggcgcg
     1801 ttgacggagc tgccggaagc gggcgtaacg ggtgcggggc gcagcacggg aatcggctgt
     1861 gcagcgcgca cttctgcggg caggcggatg gtaaagatcg atccgctccc ggcttcgctc
     1921 acgaccgtga tatcgccgcc catcatccgg cagaagcggc gtgaaatggc gagaccgagg
     1981 cccgtgccgc cgtacttgcg cgcggtggaa gcgtcggcct gcgtgaacga ctggaagagc
     2041 ttgccgcact gctccggcgt catgccgatg ccgctgtcct gcacgctgaa cgtgatc
//
LOCUS       DASURG010000274         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1479576, whole genome shotgun sequence.
ACCESSION   DASURG010000274 DASURG010000000
VERSION     DASURG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479576"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFY80_04755"
     CDS             complement(<1..565)
                     /locus_tag="VFY80_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6007248.1"
                     /translation="MDRSLFRFILRYSRRDQLRIVPFVLLTMVVYYASLDLPKTIINE
                     AIQGGRFPQPDATTGLFGLELGRLEYLFALSMIFLGLIVVNGWLKFRINTMKGWMGER
                     MLRRLRYALYDYILRFPLARFRRVKAAEMASMIKDEVEPLGGFIGEALITPLFLGGQA
                     LTAMFFILYQHWALGLIALGVVAVQAFM"
     gene            608..1432
                     /locus_tag="VFY80_04760"
     CDS             608..1432
                     /locus_tag="VFY80_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6007249.1"
                     /translation="MTFSVRFWGVRGSIACSGPRTARYGGNTSSLEVRCDERMLLFDA
                     GTGLRYLGNSLSNANTIDADLFLTHTHFDHVAGLPFFKPFFKAQNRFRLWAGHLVEGM
                     TLRRVLGEFMMSPLFPVPPQIFRARMEYREFKAGETLRPAEGIAVRTTLLNHPDGATG
                     YRVDYGGHSICYLTDTEHVPGAPDRNILGLIAGADLVIYDSMYTDAEYDAYVGWGHST
                     WQEGVRLCRAAGAKRLAVFHHDPEHDDDMLDGIAREVEKELPGSIVARDGLVVAVD"
     gene            1419..2075
                     /locus_tag="VFY80_04765"
     CDS             1419..2075
                     /locus_tag="VFY80_04765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6007250.1"
                     /translation="MQWIERYAAELEAIGSEPPPAPRWNQDWFPRLDAAAAYAMVRTV
                     KPRRIVEVGSGHSTRFLARAVADGGLDTRITAIDPKPRASLAGLDIEWIAAQVQRVAA
                     FPALGEGDILFIDSSHQLKPGSDVDFLLNAVLPLLPRGVRVHFHDIFLPDDYPEHWSW
                     RRYNEQPAVSALIKKNFLRVDFSSHATVKFHPEKIQGVLARVPLLPGAIESSLWLTKN
                     "
     gene            2057..>2304
                     /locus_tag="VFY80_04770"
     CDS             2057..>2304
                     /locus_tag="VFY80_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007251.1"
                     /translation="MADQELNIQPVLAWLDAGAPGASRPEDVLNRFCRALLACGMSLH
                     RVAVFVRTLHPNVAGRGFFWRADREEVEVDSAEHGWFGT"
BASE COUNT          396 a          763 c          737 g          408 t
ORIGIN      
        1 tcatgaacgc ctgcaccgcc actacgccga gcgcgatcag cccgagggcc cagtgctgat
       61 agaggatgaa gaacatcgcg gtcagcgcct gaccgccgag gaagagcggc gtgatgagcg
      121 cctcgccgat gaagccgccg agcggctcga cctcgtcctt gatcatcgac gccatctccg
      181 cggccttgac gcggcggaag cgcgcgagcg gaaagcgcag gatgtagtcg tagagcgcgt
      241 agcgcagccg ccgcagcatg cgctcgccca tccagccttt catcgtgttg atgcggaact
      301 tgagccagcc gttcaccacg atgaggccga ggaagatcat cgacagcgcg aagagatatt
      361 cgagccggcc gagctccagt ccgaataacc ctgtcgtcgc gtcgggctgc gggaagcgtc
      421 cgccctggat cgcctcgttg atgatggtct tgggcagatc gagcgacgcg taatagacca
      481 ccatcgtcag cagcacgaac ggcacgatgc gcagctggtc gcgcctgctg tacctgagga
      541 tgaagcggaa aaggctgcgg tccacggggg gtcccctagg gcggcatctt acaagctata
      601 gttcagcatg actttcagcg ttcgcttctg gggcgtacgc ggctcgattg cctgctccgg
      661 cccgcgcacc gcccgctacg gtggcaacac ttcttcgctg gaagtgcgct gcgacgagcg
      721 gatgctcctt ttcgacgcag gtacggggct tcgctacctc ggcaattctc tatccaatgc
      781 taatacgatc gacgcggatc tcttcctcac gcacacccat ttcgaccacg tcgccgggct
      841 acctttcttc aagccgtttt tcaaggcgca gaaccgcttc cggctctggg cggggcacct
      901 ggtggagggg atgacgctgc gccgagtgct cggcgagttc atgatgtcgc cgctctttcc
      961 ggtgccgccg cagatattcc gcgcgcgcat ggagtaccgc gaattcaagg cgggcgagac
     1021 gttgcgtccc gccgagggca tcgcggtgcg caccacgctc ctcaaccatc ccgacggcgc
     1081 caccggctac cgcgtcgact acggcgggca ctcgatctgc tacctcaccg acaccgagca
     1141 cgtgcccggc gcgccggacc gcaacattct cggcctcatc gcgggcgccg acctcgtgat
     1201 ctacgacagc atgtatacgg acgccgagta cgacgcatac gtgggatggg gacattcgac
     1261 ctggcaggaa ggcgtgcggc tttgccgcgc cgcgggcgcg aagcgccttg cggtgttcca
     1321 ccacgacccc gagcacgacg acgatatgct cgacggcatc gcgcgcgagg tggagaagga
     1381 gctgcccggg tccatcgtcg cgagggacgg cttggttgtt gcagtggatt gaacgctacg
     1441 ccgccgagct cgaagcgata ggttcggagc cgccgccggc tccgcgctgg aaccaggact
     1501 ggtttccgcg cctcgacgcc gccgccgctt atgcgatggt gcgcaccgtg aagccccggc
     1561 gcattgtcga ggtcggctcg gggcactcaa cgcgcttcct cgcgcgcgcg gtcgccgacg
     1621 gcggcctcga tacgcgcatc accgccatcg atccgaaacc aagagccagt ctggcggggc
     1681 tcgacatcga atggattgca gcccaggtgc agcgcgtcgc ggcgtttcct gcgctcggcg
     1741 aaggcgacat ccttttcatc gactcgagcc accagctcaa gcccggaagc gacgtcgatt
     1801 ttctgctgaa cgcagtgctg cccctcctgc cgcggggcgt gcgcgtgcat ttccacgaca
     1861 tcttcctgcc cgacgactat cccgagcact ggtcctggcg acgctacaac gagcagcctg
     1921 cggtttcggc cttgatcaaa aaaaacttct tgcgcgttga tttctcgagc catgccaccg
     1981 tcaagtttca tcccgagaaa atccagggcg tcctcgcgcg cgttccgctc ctgcccggcg
     2041 ccatcgagtc gagcctatgg ctgaccaaga actgaacatc cagccggtgc tggcatggct
     2101 cgacgctgga gcgccggggg cgtcgcggcc cgaggacgtg ctgaatcggt tctgccgcgc
     2161 gttgctcgcc tgcggcatgt cattgcaccg ggtcgcggtg ttcgtgcgca cgctgcatcc
     2221 caacgttgcc gggcgcggct tcttctggcg cgcggaccgc gaggaagtgg aggtcgatag
     2281 cgccgagcac ggctggttcg ggac
//
LOCUS       DASURG010000275         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_559050, whole genome shotgun sequence.
ACCESSION   DASURG010000275 DASURG010000000
VERSION     DASURG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559050"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFY80_04775"
     CDS             <1..290
                     /locus_tag="VFY80_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX6007252.1"
                     /translation="YAANFGGGQVDITNGKFVFSASEAYMVENGRIAYPVRGATLIGN
                     GPDALTRVAMIGNDMTLDPGVGTCGKEGQSVPVGVGQPTLRIDGLTIGGTV"
     gene            426..1499
                     /gene="aroG"
                     /locus_tag="VFY80_04780"
     CDS             426..1499
                     /gene="aroG"
                     /locus_tag="VFY80_04780"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEX6007253.1"
                     /translation="MLHKTDDLRIREIKELIPPSHLLREFPISENAAQTVFETRRAIH
                     RILHGADNRLLVIMGPCSIHDVEAAKEYAGRLKEARDRLASDLLIAMRVYFEKPRTTV
                     GWKGLINDPRLDGSFRINEGLRIARELLLDLNEMGVPVGCEFLDMITPQYIADLVSWG
                     AIGARTTESQIHRELASGLSCPVGFKNGTDGNIRIALDAIRAAQAPHHFLSVTKGGHS
                     AIVSTTGNEDCHIILRGGKQPNYDAASVEAAVRGLAEAGIPGRIMVDFSHGNSSKKPE
                     KQADVGREVARQIAAGQDGIFGVMAESHLKAGRQDLIPGKELVYGMSITDGCLGWEES
                     RELLDVLAAAVSKRRLKAEVEAE"
     gene            1510..2055
                     /locus_tag="VFY80_04785"
     CDS             1510..2055
                     /locus_tag="VFY80_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6007254.1"
                     /translation="MAQDEPEVAASDQDNLLRLARIGKGTALAREISGLVGRSQFFAE
                     FSRQDIAVLAEYMEVYRAEPGDIIIREGDAGDFMLLIVEGDVDILKKGARGEQQHMTS
                     VGPGMTLGEMSMIDGEPRFATCIATRPTTFAVLTRDNMAKIILDHPSLGSKILVKLVT
                     MLSLRLRQTSARLLHFLEGGA"
     gene            2045..2941
                     /locus_tag="VFY80_04790"
     CDS             2045..2941
                     /locus_tag="VFY80_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX6007255.1"
                     /translation="MARSHQLVRHRLAVLALCGIAAAPAQAVELADDRGVTVRLEAPA
                     MRIVALAPHLAEAVFAAGAGGKLVGTVLHSDYPDPVRSIPSVGDASRIDVERVMRLRS
                     DLVLAWKSGNQPSDVRRLERLGHVLFVTEPERLSDVPRLIRSIGVLAGTATRAEANAA
                     RFEREIAALRERYAGARPVAVFYEIWHRPLLTINGRHLISDVIELCGGANVFAGASFL
                     TPSVSLEALIAARPEAIVGGSSDATEGEFVREWQRHRIRTLATAALIHVDPDTMQRAS
                     LRLADGARTLCKALDRVRTPRR"
     gene            complement(2947..3507)
                     /locus_tag="VFY80_04795"
     CDS             complement(2947..3507)
                     /locus_tag="VFY80_04795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX6007256.1"
                     /translation="MAYRLSRISTRTGDDGTTGLADGKRVTKDDPRIEVIGAVDELNS
                     ALGLLLSETLPDEVRIVITSVQHALFDLGGELSLPGHVALAESRVTELDAAVERFNAE
                     LPPLREFVLPGGTRAAALAHLARAVCRRAERQLVTLSRREAVQPAALGYLNRLSDLLF
                     VLARTLNRRAGVEELLWQSSRSLNRP"
BASE COUNT          632 a         1111 c         1170 g          598 t
ORIGIN      
        1 tctatgccgc taacttcggc ggcggacagg tggacatcac gaacgggaaa ttcgtgtttt
       61 cagcctccga agcctacatg gtcgaaaacg gccgcatcgc ctacccggtg cggggggcta
      121 cgctcatcgg caacggaccg gatgcgctca cccgggtggc catgatcggc aatgacatga
      181 cgctcgatcc cggtgtcgga acttgcggca aggaagggca gagcgtgccg gtaggggtgg
      241 gacagcccac cctgcgtatc gacgggctga ccatcggcgg taccgtctga atcgagcgca
      301 gccgcgctgc gctcatcgtc tcgcgcgcag ttcggcgcgc cggcaggcgt tccactggcg
      361 gcgcgctcgc ctcgttgccg cgaagccctg ttataatgct ttgatttcac gcacactaat
      421 acaagatgct gcacaagacc gacgacctgc gcatccggga gatcaaggag ctcattcccc
      481 cgtcccacct gctgcgcgaa tttccgattt ccgagaacgc cgcgcagacg gtgtttgaaa
      541 cacggcgcgc gattcaccgc atcctgcacg gagccgacaa tcgcctgctc gtcatcatgg
      601 gcccgtgttc catccacgac gtcgaggctg cgaaggaata cgcggggcgg ctgaaggaag
      661 ctcgggaccg gctcgcgagc gatctgctca tcgcgatgcg cgtctacttc gagaagccgc
      721 gcaccacggt cggatggaaa gggctcatca acgacccgcg gcttgacgga agttttcgca
      781 tcaatgaagg cttgcgcatc gcgcgcgagc ttctcctcga tcttaacgag atgggtgtgc
      841 cggtcggctg cgaattcctc gacatgatca caccgcagta cattgccgat ctcgtgtcgt
      901 ggggcgcgat cggcgcgcgc acaacggaaa gccagatcca ccgcgagctt gcctcgggac
      961 tctcctgccc ggtgggcttc aaaaacggca cggacggcaa catccggatc gcgctcgacg
     1021 caatccgcgc cgcgcaggct ccgcatcatt ttctttccgt caccaaaggc ggccactcag
     1081 ccatcgtctc caccaccgga aacgaggact gtcacatcat cctgcgcggc ggcaagcagc
     1141 cgaactatga cgcggcgagc gtggaagccg cggtgcgcgg cctggcggaa gccggcatac
     1201 ccgggcgcat catggtcgat ttcagtcatg gcaacagcag caagaaaccg gagaagcagg
     1261 ccgatgtggg ccgcgaagtg gcccgtcaga tcgccgcagg gcaggacggc attttcggcg
     1321 tcatggcgga aagtcatctg aaggcgggcc gccaggatct catcccgggg aaagagctgg
     1381 tgtacggaat gagcatcacc gacggctgcc tgggctggga ggagagccgc gagctgctcg
     1441 acgtgctcgc cgctgcggtc agcaagcggc gtttgaaggc agaggtcgag gcggagtagt
     1501 cccttgccga tggcacagga cgagcctgaa gtcgccgcat ccgaccagga taacctgctg
     1561 cgtctcgcaa gaatcggcaa gggcacggct ctcgcgcgcg aaatctccgg gctcgtcggg
     1621 cgctcgcagt tctttgccga gttttcccgt caggacatcg ctgtgctcgc cgagtacatg
     1681 gaggtgtatc gtgcggagcc cggtgacatc atcattcgcg aaggcgatgc gggcgatttc
     1741 atgctgctca tcgtcgaagg cgacgtcgac atcctgaaaa aaggcgcgcg cggcgagcag
     1801 cagcacatga ccagcgtcgg tcccggcatg acgctcggtg aaatgtcgat gatcgatgga
     1861 gagccgcggt tcgccacctg catcgcgacg cggccgacca cgttcgccgt gctgacgcgc
     1921 gacaacatgg cgaagatcat cctcgatcac ccgagcttgg gttcgaaaat ccttgtcaag
     1981 ctcgtcacga tgctgtcgct ccgcctgcgg caaaccagcg cacggctgct gcatttcctg
     2041 gaaggtggcg cgtagccacc agctcgtgcg tcacaggctc gctgtcctcg cgctgtgcgg
     2101 catcgcggcg gcgcctgctc aggcggtgga gctggcggac gatcgcggcg ttacggtgcg
     2161 gctcgaggcc ccggcgatgc gcatcgtcgc gctcgcgccg catcttgccg aggccgtatt
     2221 tgccgcaggg gcgggtggga agctcgtggg caccgtgctt catagcgact atcccgatcc
     2281 cgtgcgctcc atcccgagcg tcggagacgc ctctcgcatc gatgtcgagc gcgtcatgcg
     2341 actgcggtcc gatcttgtgc tcgcctggaa aagcggtaac caaccgagcg acgtgcggcg
     2401 cctcgagcgt ctcggccatg tacttttcgt caccgaaccc gaacggctca gcgacgtgcc
     2461 tcgactcatc cgcagcatcg gcgttctcgc cggaacggcg acgcgcgcgg aggcgaatgc
     2521 ggcccgcttc gagcgggaaa tcgctgcgct gcgcgaacgc tatgctggcg cgcggcccgt
     2581 cgcagtgttc tacgaaatct ggcaccggcc actgctcacg ataaacggcc gtcacttgat
     2641 cagcgacgtc atcgaactgt gtggaggcgc gaacgtgttt gccggcgcgt ctttcctgac
     2701 gccgagcgtc tcgctcgaag ccttgatcgc cgcgaggcct gaagcgatcg tgggcggatc
     2761 ctcggatgcc accgaaggcg agttcgttcg cgagtggcag cgccatcgta tccggacact
     2821 ggccactgcc gcgctcattc acgtcgatcc ggataccatg cagcgggcga gcctgcgcct
     2881 cgcggacggc gcgcggacgc tgtgcaaggc gctggatcga gtaagaacgc cgcgtcggta
     2941 ggcgctttag ggccggttca agctgcggct ggactgccac agcagctctt cgactcccgc
     3001 gcgacggttg agggttcgcg ccagcacgaa cagaaggtcg gaaaggcgat tgagataccc
     3061 gagcgcagcg ggctgcactg cctcacgccg ggagagggtg acgagctgcc gttcagcgcg
     3121 cctgcagacc gcgcgggcaa gatgtgcgag cgctgcggcg cgcgtgccgc ccgggaggac
     3181 gaactcgcga agcggaggca gttcggcgtt gaagcgctcg acagcggcgt cgagctccgt
     3241 cacgcgggat tccgcgagcg cgacgtggcc gggaaggctc aactcgcctc cgagatcgaa
     3301 aagcgcgtgc tgcacggagg tgatgacaat gcgcacttcg tcggggagcg tctcggagag
     3361 gagcagaccg agggcgctgt tcagctcgtc cacggcaccg atcacttcga tgcgcgggtc
     3421 gtccttcgtc acgcgcttgc cgtcggcgag cccggtggtg ccgtcgtctc ccgtgcgggt
     3481 ggagatgcgg gacaatcggt aggccatgct t
//
LOCUS       DASURG010000276         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_762681, whole genome shotgun sequence.
ACCESSION   DASURG010000276 DASURG010000000
VERSION     DASURG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762681"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..909)
                     /locus_tag="VFY80_04800"
     CDS             complement(49..909)
                     /locus_tag="VFY80_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6007257.1"
                     /translation="MSALALALVLAAALVHATWNYVLKRSGGGTLFVWWFAALSAVLY
                     APLALAVVWWVAPEIRWMHFGLMLASAVLHTGYYMLLDRGYRYGDLSLVYPLARGSGP
                     LITVLCAVVLLREDPGVVALTGALLVCGGAVLLTGNLAKLRERGSLPAVGFALLTGCM
                     IASYTLVDKIAVAAYLIPPILQDWAANLGRVALMTPLALRAKREIMPTWRRSRNAIIA
                     VAILCPLSYILVLTAMVFTPVSYIAPAREISILVATAMGTQLLAEGDAPRRLAAALTM
                     VIGVVCLAVG"
     gene            complement(940..>2458)
                     /locus_tag="VFY80_04805"
     CDS             complement(940..>2458)
                     /locus_tag="VFY80_04805"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX6007258.1"
                     /translation="MPNLPGTIIAMLATTSLGAIWSSCSPDFGVQGVLDRFGQIEPKV
                     LVSADGYFYSGKRIDVRARLKDIMGALPSVERLIIVPYTERAPRIEDIPRAINVHAFL
                     APYAPRDIRFARLPFDHPLYIMYSSGTTGVPKCIVHGAGGTLLQHLKEHVLHADLKPG
                     NRMFYFTTCGWMMWNWLVSALAARATVLLYDGSPFIMDGRVLFDYADRERMTVFGTSA
                     KYIDAVAKLGLAPRRSHRLDTVTTMLSTGSPLAPESFDYVYRDIKSDIQLSSISGGTD
                     LIASFAMANPLLPVWRGELQCRGLGMKVEVYDEEGRPVVGQKGELVCTAPFPSMPVSF
                     WNDPDGAKYRAAYFEKFPGVWCHGDYVELTPRGTLIIYGRSDAVLNPGGVRIGTAEIY
                     RQVERLDEVVESLAVGQQWENDVRIVLFVRLRDGLVLDEALSERIRREIRTHATPRHV
                     PARIVQVPDIPRTKSGKIVELAVRNVVHGLPVKNVEALANPEALAHFASRPELQS"
BASE COUNT          480 a          773 c          814 g          391 t
ORIGIN      
        1 cacctacaga tctggaactg gtgatgccgt gcacgtcccg atgcggcgct agccgactgc
       61 cagacatacg acgccgatga ccatggtcaa cgccgcggcg agacgccggg gcgcatcacc
      121 ttccgccagc aactgggtgc ccatcgcggt cgcaaccagt atgctgatct cgcgtgccgg
      181 cgcaatatag ctgaccggcg taaacaccat tgcagtcagc acgaggatgt aggacagcgg
      241 gcagaggatc gccacggcaa tgatcgcatt gcgcgagcgg cgccaggtgg gcatgatctc
      301 gcgcttcgcg cgcagcgcga gcggcgtcat gagtgcgacg cgcccgaggt tcgcggccca
      361 gtcctgcagg atcggtggaa tgaggtacgc tgccaccgcg atcttgtcga cgagcgtata
      421 gctcgcgatc atgcaaccgg tgagcagcgc gaatccgacc gcgggcaggc tgccgcgttc
      481 gcgcagtttt gcaagattgc cggtgagcag caccgcacct ccgcagacaa ggagcgcgcc
      541 ggtcaacgcg acgacgcccg gatcctcgcg caggaggacg actgcgcaca gcacggtgat
      601 gagcgggccg ctgccgcgag cgagcgggta gacgagcgaa agatcgccat agcgatagcc
      661 cctgtcgagc agcatgtagt agccggtatg cagcacagcg ctcgcgagca tgagcccgaa
      721 gtgcatccac cgtatctcgg gcgcgaccca ccacaccact gcgagcgcga gcggcgcata
      781 gagcaccgcc gaaagcgccg caaaccacca cacgaagagc gttccgccgc ccgagcgctt
      841 caggacgtaa ttccaggtgg catgcaccaa ggccgcagcg agcacgagcg ccagtgcgag
      901 ggcgctcatg atcgcgggat aggcatggcg cgatgcgcct cagctctgaa gttcgggacg
      961 cgaagcgaag tgcgcgagcg cctccggatt cgccagcgct tcgacattct tcacggggag
     1021 gccgtgtact acattgcgta ccgcgagctc gacgatcttg ccgctcttgg tgcgcggaat
     1081 gtccgggacc tggacgattc ttgccggcac gtggcgcggt gtcgcatgag tcctgatctc
     1141 gcggcggatc cgctcggaga gcgcctcgtc cagcacgagc ccatcgcgga gccgcacgaa
     1201 gagcacgatg cggacatcgt tttcccactg ctggccgaca gcgagacttt ccaccacctc
     1261 atcgaggcgc tccacctgcc gatagatctc cgccgttccg atacgtaccc cgccgggatt
     1321 gagcacggca tccgagcgcc cgtagatgat gagcgtccca cgcggggtca gctcaacata
     1381 gtcgccgtgg caccacacgc cgggaaactt ctcgaagtag gctgcgcggt acttggcgcc
     1441 gtccgggtcg ttccagaagc tgactggcat ggacggaaac ggcgcggtgc acacgagctc
     1501 gcccttttgc cccaccacgg ggcgaccctc ctcgtcgtag acctcgactt tcatgccgag
     1561 gccgcggcat tgaagttccc cgcgccacac cggtagcagc ggattcgcca ttgcaaacga
     1621 ggcgatgaga tcggtgccgc ccgatatgga cgagagctgg atgtcgctct tgatgtcgcg
     1681 atagacatag tcgaagcttt cgggcgcaag gggagagccg gtggaaagca tcgtggtcac
     1741 cgtatcgagc ctgtggctgc gacgtggcgc gaggccgagc ttggcgacgg catcgatgta
     1801 tttcgccgag gtcccgaaca ccgtcattct ttcgcggtcg gcatagtcga agagcacccg
     1861 accgtccatg atgaacggcg agccgtcgta gagcagcacc gttgcgcgcg cggcgagcgc
     1921 ggataccagc cagttccaca tcatccagcc gcaggtggtg aagtagaaca tgcgattgcc
     1981 gggcttgaga tccgcatgca gcacgtgctc cttcagatgc tgcaggaggg tgccgcccgc
     2041 gccatgcacg atgcatttgg gcacgccggt ggtacccgag gagtacatga tgtagagcgg
     2101 atgatcgaag ggcaggcgcg cgaagcggat atcgcgcggc gcgtatggcg ccagaaaagc
     2161 gtggacgttg atcgcgcgcg gaatgtcttc gatgcgggga gcgcgctcgg tgtagggcac
     2221 aatgatcagc cgctcgacag aaggcagcgc tcccatgatg tccttgagcc gcgcccgcac
     2281 gtcgatgcgc ttgccgctgt agaagtaacc gtccgcgctg acgagcacct tgggctcgat
     2341 ctgcccgaag cggtccagca cgccctgcac gccgaagtcg ggagagcacg aagaccagat
     2401 tgcgccgagg ctcgtcgtgg cgagcatcgc gatgatcgtt ccgggcaagt tgggcatg
//
LOCUS       DASURG010000277         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_968059, whole genome shotgun sequence.
ACCESSION   DASURG010000277 DASURG010000000
VERSION     DASURG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968059"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VFY80_04810"
     CDS             <1..1096
                     /locus_tag="VFY80_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6007259.1"
                     /translation="PAGMCYSSGTTGKPKGVVYSHRSIALHSLATATVDYMGVSHADA
                     VCPVVPMFHVNAWGIPYTAVMMGSKLVFPGPHLDAESLLDLYESERVTLTAGVPTIWM
                     SMLELLETSRGRWRLMPGMRMVVGGAAASEAMIRRYDRLGLRVVHGWGMTETSPVGTV
                     NCMKRELEGASEDERYAIRARQGLPLPFFEIRAMAADGEAPWDGATMGELQVRGPWVA
                     ARYHNPEFEPHQWTEDGWFCTGDIATIDPSGYVKIADRIKDLIKSGGEWISSIDLENA
                     LASHPKVAEVAVIAIAHSKWGERPLAIIVPQRGQTVTASELRAHLAPRVARFWMPDGF
                     VFVTEMPRTSTGKVLKSTLRERYRDGPAEI"
     gene            1227..1829
                     /locus_tag="VFY80_04815"
     CDS             1227..1829
                     /locus_tag="VFY80_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX6007260.1"
                     /translation="MGKRRLATALAQYRERVPDAALPAVRWLPFQLNPDLPEEGIARA
                     EYLRRKFGNPNRSYGHVAQVGASVGIPFAFERITVQPNTLHAHRLMHYADQRGRQDEM
                     AETLFQAYFIEGANLADRTVLAEVGARAGFERGALAEYLASDADRELIARADSDARNA
                     GIGGVPFFIFNRRIGVSGAQEPETLLEAMLQAEKEASTAA"
BASE COUNT          366 a          661 c          689 g          376 t
ORIGIN      
        1 cccagcgggc atgtgctatt cctctggcac gaccggaaaa ccgaagggtg tggtgtactc
       61 gcaccgttcc atcgcgctcc actcgctcgc gaccgcgacg gtcgattaca tgggcgtctc
      121 gcatgcggac gcggtgtgcc cggtggtccc gatgtttcac gtcaacgcct ggggtattcc
      181 ctacactgcc gtgatgatgg gttcgaagct cgtcttcccg ggcccccatc tcgacgcgga
      241 aagcctgctc gacctctacg agtcggagcg cgtcacgctc accgctggcg tgcccaccat
      301 ctggatgagc atgctggaac ttctggaaac gtcgcgcggt cgatggcggc tcatgcccgg
      361 catgcggatg gtggtgggcg gtgccgcggc atccgaagcg atgatccgcc gttacgatcg
      421 cctcggtctg cgggtcgttc acggctgggg catgacggaa acgtcccccg tcggtacggt
      481 gaattgcatg aagcgtgaac tcgagggcgc gagcgaagac gaacgctacg ccatccgtgc
      541 gcgccagggc ttgccgcttc cttttttcga aattcgtgcg atggccgcgg acggagaagc
      601 gccatgggac ggtgcaacga tgggagagtt gcaggtgcgc ggaccatggg tcgcggcgcg
      661 ctatcacaac cccgagtttg agccgcatca gtggaccgag gatggatggt tttgcaccgg
      721 cgacatcgcg acgatcgacc cctcgggcta cgtcaagatc gccgatcgca tcaaggatct
      781 catcaagtcg ggtggcgaat ggatcagctc catcgacctg gagaacgccc tcgcaagcca
      841 tcccaaggtg gcggaagtgg cggtcatcgc gattgcgcat tccaaatggg gcgagcggcc
      901 tctcgctatc atcgtgccgc aacgagggca aacagtgacg gcgtcggaac tgcgggcgca
      961 cctcgcgccg cgcgttgcgc gcttctggat gcccgacggg ttcgtattcg tcactgaaat
     1021 gccgcgcacc tccaccggaa aggtgctgaa gtccacgctg cgggagcgtt accgcgacgg
     1081 gccggcagaa atctgaatac cctcgttcgg gcgccggcag gcattactgc tatgatgagg
     1141 cgggtctggt atatcgttct tcgaggagaa atgcgatgag cctcagaatc gacatcgttt
     1201 cagacgtggt ctgaccttgg tgctacatcg ggaagcgccg actcgccacg gcactcgccc
     1261 agtatcgcga gcgcgttccc gatgccgcat tgccggccgt gcgctggcta ccgtttcagc
     1321 tgaatcccga tctgccggag gaaggcatcg cgcgcgccga gtatctgcgt cgcaaattcg
     1381 gcaatcccaa ccgcagctac ggccacgtcg cccaggtggg cgcgagcgtc ggcatacctt
     1441 ttgccttcga gcgcattacc gttcagccca atacgctcca cgcgcatcgt ctgatgcatt
     1501 atgccgacca gcgcggccgg caggatgaaa tggccgaaac gcttttccag gcgtatttca
     1561 tcgaaggcgc aaacctcgcc gaccgcacgg tgcttgccga agtgggggca cgggcgggct
     1621 tcgagcgcgg ggcgctcgcg gaatatctcg cgagcgatgc cgatcgcgaa ctcattgcgc
     1681 gtgccgactc ggacgcgcgc aacgccggaa tcgggggcgt gccgttcttc atcttcaatc
     1741 gccgcatagg ggtctcaggc gcgcaagagc cggaaacgct gctcgaggcg atgttgcagg
     1801 cggaaaagga agcctccacg gcggcgtgac ggcgccgtca gcgcatgaag ccgcggcgtc
     1861 cttggcgatg ggtcctggcg gcagcggtta tcgtgctggc cgtgcttgcg ctctggtatg
     1921 gccgcacgcc gccggcacca ctcccacgcg acggggcggt cacgatcccg cgccctcacg
     1981 acgaccgctc gggtgccgca tctcgtgaac ggccgtcccg cgagcgcagc caaaggccct
     2041 gatcagcggc tgatccacgc gtagagcgcc gtgtcgatct cgggacggtg cg
//
LOCUS       DASURG010000278         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_355500, whole genome shotgun sequence.
ACCESSION   DASURG010000278 DASURG010000000
VERSION     DASURG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355500"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFY80_04820"
     CDS             complement(<1..618)
                     /locus_tag="VFY80_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6007261.1"
                     /translation="MPKTTGPRFVADALRGYGVSHVFFVPQMLLETLTAMEGMGIRRV
                     MTHGEKAAAYMADGYARASGRPGVCMAQHVGASNLAAGLKDAYLASSPVIAITGGPAP
                     LARHRHAYQELEDFTQFEPTTKFNAHLDHIARLPDLMRQAFREATTGCPGPVHLQIAG
                     HHAESIMTEGELELLVEEQHKEVPAYRPAADPAHVTAALRLLEAAE"
     gene            827..1453
                     /locus_tag="VFY80_04825"
     CDS             827..1453
                     /locus_tag="VFY80_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6007262.1"
                     /translation="MPRAPKVDALIFDFGNIVVDIDFARVFEAWGRAAGVPAPDIARR
                     FAFDAAYQAHELGEIESADYFAALRRSCGVELTDDDFLAGWNAIFVAPRAGMDELLAT
                     LAARLPLYLFSNTNALHQAYWSAQYREILRHFTRIYSSHELGLRKPSPAAFTKIARDV
                     GIAPSRLAFFDDTAENVEGARQAGLHAFHVTSVPQLRDVLENELHIPR"
     gene            complement(1543..2772)
                     /locus_tag="VFY80_04830"
     CDS             complement(1543..2772)
                     /locus_tag="VFY80_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6007263.1"
                     /translation="MKDATLGAYDIADLREMARRRLPKGIFEFMDRGNGDEVALAHNR
                     AAFERFKFNPHVLVDTSRRTLETTLFGRKHAMPIAIAPTGSAGLTWFQGEIALARAAR
                     EAGIPFALATGSMTALEKVAEEAGGSLWFQVYMWPDRSLSHRLIERAKAAGYEALIVT
                     VDTPVTPGREYNLRNGVTIPFRFTRRNVTDVLMHPRWIVNVLLRYILTSGMPRYENYP
                     TQMKQRITALPMGRSMMVTDSLTWDDLRALRKLWPHPLMVKGILRAEDAKQAADCGAD
                     GVIVSNHGGRAVDSTMAPIEVLPRIVDAIGKRLTVLADSGFRRGADVVKGLALGASAV
                     LIGRATIYGTALAGQAGAARAIEIYRDEIDRLLALIGCPGVAELDRDFLVMPPEYDAS
                     PPPNLSFINEARAAAER"
BASE COUNT          484 a          957 c          937 g          515 t
ORIGIN      
        1 ctcggcggcc tcgagcaggc gcagcgctgc cgtgacgtgc gcgggatcgg ccgcggggcg
       61 atacgcgggc acctccttgt gctgctcctc gaccagaagc tccaactccc cttcggtcat
      121 gatcgattcc gcatggtggc ccgcgatctg gaggtgcact gggcccgggc atccggtggt
      181 cgcctcgcga aacgcctgcc gcatcagatc gggcagccgc gcgatgtgat cgaggtgcgc
      241 attgaacttg gtggtgggct cgaactgcgt gaagtcttcg agctcctgat aggcatggcg
      301 atggcgcgcg agcggcgccg gcccgccggt gatcgcgatc acgggcgacg aggcaagata
      361 ggcatccttg agtcccgccg cgagattgga cgcgccgaca tgctgcgcca tgcaaacgcc
      421 cggtcttccg ctcgcgcgcg catagccgtc cgccatgtac gccgccgctt tttcgccgtg
      481 cgtcatcacg cggcgtatcc ccatgccttc catggccgtc aacgtctcga gcagcatctg
      541 cggaacgaag aagacgtgac ttacgccata cccgcgcagc gcgtcggcga cgaatcgcgg
      601 gccggtcgtt ttaggcattg cgtgactctc ccccgggacg gaacggttta gagggtgcga
      661 tcgaggtgtc ttctggaaga cgctgctgcg gtcgacgtgc gaacgatgct aacaaagcgc
      721 atgcgcgact tcaacaaagc gcattcgggc tcacaatgcg gacacccgct cccgcacgcc
      781 cgattcgagc attgccgcag gtccccgcag ccggtaacgt agctgcatgc cccgtgcgcc
      841 aaaggtggac gcgctgatct tcgatttcgg caacatcgtc gtcgatatcg atttcgcgcg
      901 cgtgttcgag gcgtggggac gcgcggccgg cgtgccggcg ccggacatcg cacgacgctt
      961 cgcgttcgat gccgcgtatc aggcgcacga gctcggcgag atcgaaagcg ctgattactt
     1021 cgccgcgctg cgccgctcgt gcggcgtcga gctgaccgat gacgattttc tcgccggctg
     1081 gaacgcgatc ttcgtggcgc cgcgcgccgg gatggacgag ctgctggcta cgctcgccgc
     1141 acgcctgccg ctctatcttt tctccaacac gaatgcgctc caccaagcgt actggagcgc
     1201 gcagtaccgc gaaatcctgc gccacttcac gaggatctac tcctcgcacg agctcggcct
     1261 gcgcaaaccg tcgcctgccg cgttcacgaa aatcgcacgc gacgtcggca tcgcgccttc
     1321 gcggctcgcg ttctttgacg atacggcgga gaacgtcgaa ggcgcgcggc aggcgggtct
     1381 ccatgcgttt catgtcacat ccgttccaca attgcgcgat gtgctggaga acgaactcca
     1441 tattccgcgc taatgggtag ggtgggcgct tgagtccacc gtcggtaccg cgggcggtgg
     1501 gcatgaatgc ccaccctaca acgaactcca ccagatttca cttcagcgct ccgcggcggc
     1561 tcgtgcctcg ttgatgaagc tcaggttagg tgggggcgag gcatcgtact ccggcggcat
     1621 cacgaggaag tcgcgatcga gctccgcgac tcccggacag ccgatcagcg cgagcagccg
     1681 atcgatttcg tcgcgataga tctcgatcgc gcgtgcggcg cccgcctgtc ccgccagcgc
     1741 ggtgccgtag atggtcgcgc ggccgatcag cacggcgctc gcgccgagcg cgagcccctt
     1801 gacgacgtcc gcgccgcggc gaaaaccgct gtccgcgagg acagtgagac gcttgccgat
     1861 ggcatcgacg atgcgcggca gcacttcgat tggcgccatc gtgctgtcca ccgcgcgccc
     1921 gccgtgattc gaaacgatga cgccgtcggc gccgcagtcg gcggcctgct ttgcgtcttc
     1981 cgcgcgcagg atgcccttta ccatcagtgg atgcggccag agcttgcgca gcgcccgcaa
     2041 gtcgtcccac gtgagcgaat cggtcaccat catcgaacgg cccatgggca gcgccgtgat
     2101 gcgctgcttc atttgcgtgg ggtagttttc gtagcgtggc atgccgctcg tcaggatgta
     2161 gcgcagcagc acgttcacga tccagcgtgg atgcatcagc acgtcggtga cattgcggcg
     2221 cgtgaagcga aacggaatcg tgacgccgtt acgcaggttg tactcgcgac ccggggtgac
     2281 gggcgtatcg acggtgacga tgagcgcctc atagcccgct gccttggcgc gctcgatcag
     2341 ccgatgggaa agcgaacggt cgggccacat gtacacctgg aaccagagcg agccgcccgc
     2401 ctcctccgcc actttttcga gggccgtcat ggatccggtc gcaagcgcaa atggaatccc
     2461 cgcctcgcgc gccgcacgcg ccagcgcgat ctcgccctga aaccaggtaa gcccggcgga
     2521 acccgtcggc gcgatcgcga tcggcatcgc gtgcttgcgg ccgaagagtg tggtctccag
     2581 cgtgcgccgc gacgtatcga ccagcacgtg cggattgaac ttgaagcgct cgaacgcagc
     2641 gcggttgtgc gcgagcgcca cttcgtcgcc gttgccgcgg tccatgaatt cgaagatgcc
     2701 tttcggcagg cggcggcgcg ccatctcgcg cagatccgcg atgtcgtatg cgccgagggt
     2761 cgcgtctttc atggtaattt cgcgagcgcc gtcttcccgg cgaaaaccgc gtgccccgtc
     2821 ttcccggcaa aagccgggat ccaggatgac ccatcaaacg ccgggctgga tatcggctcg
     2881 cgccgatatg acg
//
LOCUS       DASURG010000279         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_763370, whole genome shotgun sequence.
ACCESSION   DASURG010000279 DASURG010000000
VERSION     DASURG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763370"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..2202)
                     /locus_tag="VFY80_04835"
     CDS             complement(106..2202)
                     /locus_tag="VFY80_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6007264.1"
                     /translation="MTDLSFLLAPRSIAILGASDDFSKVNGRTVKYLLAKGYAGSIYP
                     VNPKYERIGELPCYPAVDALPEPPDLAVVGLPATLVVQAVSDLGRRGVKSAIVFSSGF
                     AEIGEAGRALEQQVVAAAKAAGVRLCGPNCLGLINAFSNVMATFGQFAEGDTPPGPVA
                     FVTQSGAFGTAIAALARRRGMGLGYFVNTGNEGDIDFVEIMDAVLHDERIKVGAGYIE
                     GLKDGARFREMAERALALGKPLVVTKVGRTQAGARAAVSHTGSLAGADEVFSGVVHGL
                     GVLRARNEEQMLDMVEAFAHCALPQGKGLGILTQSGGAGVLMADHAEDCGLSVPVLSK
                     TTQNALRKVMPPFGASGNPVDITGQFIAEPGLLERAFRIVLSDPDVHIGIVWLQLMEG
                     YVDVLVGIFERLKAQSEKPFLVCWVAAPDGAAKALRESGIPVLRGAEPAVDAAGALAR
                     YARARRDWLADSEARAALRHTCVHAPLERAASGPVPTVIAASWLDKWGVATAPIRLAH
                     TAEEAVLASGDLGYPVAVKIESPDIPHKTDAGGVKLMLQDAESVRQAYGEVLSDVRRL
                     QPSARIEGVVVQRMAKPGVELVVGLKRDPVFGMLIMVGLGGIHIEILKDVAFRAAPVT
                     QHEAGQMLAELRGSAVLEGARGQPPADRRALCRFISAVSAFGAAAGKQLRELDLNPVF
                     ATAEGVVAVDWLLVVE"
     gene            complement(2296..3786)
                     /locus_tag="VFY80_04840"
     CDS             complement(2296..3786)
                     /locus_tag="VFY80_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6007265.1"
                     /translation="MISSKELLDRTGISRATLNNYIAMGLLPRPLVAAPGQDEDSRAR
                     QLGYFPDSAIDRVHQIQRLKREGYTMAEIVTQLVAEAGRAETSAAPETVTRAAQLPIT
                     EAPRVTIDGIAHPAFMVNYNFEVIWFNRSAQTLLLGRDVQLPSTSEGRNLFRLLLGQP
                     QSERNRSALLRFHLSLAKERLSKATLMSFCHGVPPERLALLDRMYAEIEPEEARPILD
                     APLQLDSDNGQTTAYRMYAAYFREGILLVLMPEGIESETLLHFLARRDEVIRSLLRKR
                     LPVLTDLAVFVADLQNSVKICSELPPEEYFELINEICSTMAPIFRKYFATHGKHAGDG
                     LLYYFFPQPDSNYIFNALRCARELRSEIQKISKAWQIRKNWLNELYLNIGLTEGQEWL
                     GTFQSATSVEFIVLGDSINQASRISDFARFGAIWATKSFMGKLSTEERLKVRFGVTRH
                     TPEGREVFVSSSYATVSALVSLENARNDKLRDIATLPVTEVVEVAL"
     gene            complement(3981..4427)
                     /locus_tag="VFY80_04845"
                     /pseudo
     CDS             complement(3981..4427)
                     /locus_tag="VFY80_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(4511..4723)
                     /locus_tag="VFY80_04850"
     CDS             complement(4511..4723)
                     /locus_tag="VFY80_04850"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX6007266.1"
                     /translation="MLAKLIDHIIENTEDVGPAFPEEARKIHYGESEERRIRGTASTQ
                     DVEALREEGIEVAALPVPLHRLGKSH"
BASE COUNT          919 a         1485 c         1448 g          918 t
ORIGIN      
        1 tgacagccga tagcgtgcgc gaatgcaggc acggcgtggc gattactgcg ccgcgaatca
       61 catcggcgac ttgcctcact ctcgacgcat cgggtccgag ggtgctcatt cgacgacaag
      121 cagccaatcg acggcgacca caccttcggc tgtcgcgaag acgggattga gatcgagttc
      181 gcgcaactgc ttaccggctg ccgccccgaa cgcggacacc gcgctgatga acctgcataa
      241 cgctcggcga tccgccggag gttgcccccg cgcgccctca agcacggcgc ttcctcgcag
      301 ttcagcgagc atctgcccgg cttcgtgctg ggtcaccggt gccgcgcgaa acgcaacatc
      361 cttcagtatt tcgatgtgaa tgccgccgag cccaaccatg atgagcatac cgaataccgg
      421 atcgcgcttg aggcccacga ccagctcaac acccggcttc gccatccgtt gcacgactac
      481 accttcgatg cgtgcggatg gttgcaaccg gcgcacgtcg gacaggactt caccgtaggc
      541 ctgcctcacg ctttccgcgt cctgcagcat gagctttacg ccgcccgcgt cggtcttgtg
      601 cgggatgtca ggcgattcga tcttcactgc gaccgggtag ccgaggtctc cacttgccag
      661 aaccgcctct tccgccgtat gcgcgagccg gatcggcgcc gtcgcgacgc cccacttgtc
      721 gagccaggat gccgcgataa cggtagggac gggtccggac gccgcgcgct cgagcggtgc
      781 gtgaacgcag gtatggcgaa gcgccgcgcg cgcttcagag tcggcaagcc agtcgcggcg
      841 tgcgcgtgca taccgcgcca gcgcgccggc cgcatcgact gcgggttcgg cgccgcgcag
      901 caccggaatg ccgctttcgc gcaaggcctt tgctgcgccg tccggcgcag ccacccagca
      961 caccagaaac ggtttctcgc tttgcgcctt caggcgttcg aaaatcccga cgagcacgtc
     1021 gacatacccc tccatgagct gcagccatac gattccgata tgcacgtcag gatccgagag
     1081 cacgatcctg aaagcccgct ccagaagccc gggttccgcg atgaactggc cggtgatatc
     1141 gaccgggttg ccgctcgcgc cgaagggcgg catgaccttg cgcagcgcat tctgcgttgt
     1201 cttggaaagc acggggaccg acaaaccgca gtcctcggca tggtctgcca tcagcacgcc
     1261 ggcgcctccg gattgagtga ggatgcccag cccctttccc tgcggtagcg cgcaatgcgc
     1321 gaaagcttcc accatgtcga gcatctgttc ttcattgcgt gcccgcagta cgccgagacc
     1381 gtggacgacg ccggaaaaaa cctcatccgc gccggccagg gatccggtgt gcgaaacagc
     1441 ggcgcgggca cccgcctggg ttcgtccaac cttcgtaacg accagcggct ttccgagcgc
     1501 gagcgcgcgt tccgccattt ctcgaaagcg cgcaccgtct ttcaagcctt cgatatagcc
     1561 tgcgcccact ttgattcgct cgtcgtggag cacggcatcc atgatctcga caaaatcgat
     1621 gtcgccttcg ttgcccgtat tgacgaaata gcccaatccc atgccgcggc gccgggctaa
     1681 ggcggcaatc gccgttccga acgcgcccga ctgcgtaacg aatgcaaccg gccccggggg
     1741 tgtatcgccc tccgcaaact gcccaaaagt cgccatgacg ttgctgaatg cgttgatcag
     1801 cccgaggcag ttcggaccac acaagcgcac acccgcggcc ttcgcagctg cgaccacctg
     1861 ttgctccagc gcccgtccgg cttcgccgat ctcagcgaat ccggaagaga agacgattgc
     1921 cgacttgacg ccgcgccgac ctaagtccga aacggcttgg acgacgagcg tggcgggtaa
     1981 cccaacgacg gcgagatcgg gtggttcagg cagcgcatca accgcgggat aacacggtag
     2041 ctcgcctatc ctctcgtatt tgggattcac aggatagatc gagccggcat atcccttcgc
     2101 gagtaggtac ttgacggtgc ggccattcac cttggagaaa tcatccgagg cccccagaat
     2161 ggcgatggag cgtggggcga gcagaaagga aagatcggtc accggattag tgggaaatgc
     2221 ttcgcgcggc agcccgccgc tcgagccgca attacaccac aaagccgccg gagagcggca
     2281 gcaccgccgc ggcggctata gcgcgacttc gaccacctcg gttacaggca gcgtggcgat
     2341 gtcgcgaagc ttgtcgttgc gcgcgttctc caggctcaca agcgccgaga ccgtcgcata
     2401 gctcgacgag acgaagactt cccgtccttc aggcgtgtgg cgggtgacgc cgaaacgcac
     2461 tttgagccgc tcttccgtgg aaagcttgcc cataaagctc tttgtcgccc agatcgcccc
     2521 gaagcgcgcg aagtcgctga tacgcgaagc ctgattgatg ctgtctccga gcacgatgaa
     2581 ttccacgctg gttgcggact ggaaggtgcc cagccactcc tgcccttcgg tgagcccgat
     2641 gttgagatac agctcgttca gccagttctt ccggatctgc cacgccttgc tgatcttctg
     2701 tatttcggag cgtagctcgc gcgcgcatct cagggcattg aatatgtaat tggaatccgg
     2761 ctgcggaaaa aagtaataca gcaggccgtc gcccgcgtgt ttgccgtgcg tagcgaaata
     2821 cttgcggaat atcggggcca tggtgctgca gatctcgttg atcagctcga agtactcctc
     2881 gggcggcagt tcggagcaga tcttgacgga gttctggaga tcggcgacga agaccgcgag
     2941 atcggtcagt accggcagcc gcttgcgcag cagcgagcgg atgacttcgt cccgtcttgc
     3001 gaggaagtgc agcaaggttt cgctttcgat gccctcaggc atgaggacga gcagaattcc
     3061 ctcgcggaaa tacgcggcat acatgcggta cgccgtcgtt tgcccgttgt cactgtcgag
     3121 ctgcagaggc gcatcgagaa tgggccgcgc ctcttccggc tcgatctcgg catacatccg
     3181 atcgagcagt gccaagcgtt ccggtggtac gccgtgacag aacgacatca gcgtggcctt
     3241 tgagagccgt tccttcgcaa gcgagagatg gaagcggagc agcgccgagc gattgcgctc
     3301 cgattggggt tgaccgagaa gaagccggaa gagattgcgg ccttcgctcg tcgaggggag
     3361 ctggacgtcc cgtccgagaa ggagggtctg agccgaccga ttgaaccaga tcacttcaaa
     3421 gttgtagttc accatgaagg caggatgagc gatgccatcg atggtgacac gtggcgcctc
     3481 ggtgatcgga agttgcgcgg cgcgcgtaac cgtctccggg gcagccgatg tctctgcacg
     3541 ccctgcttcg gcaacgagct gagtgacgat ttcggccatg gtgtaaccct ctctcttcag
     3601 gcgttgtatc tggtggacgc ggtctatggc gctgtccgga aagtagccga gctggcgggc
     3661 ccggctgtcc tcgtcctggc ccggggccgc caccagcggg cgcggcagca gtcccatggc
     3721 gatgtagtta ttaagcgtcg ccctcgagat accggtgcga tccaggagct ctttgctcga
     3781 aatcattcta gttatggtct tatttggtct gtctgtttag acagtatata cagtccaatg
     3841 aagaggtcaa catgaagttg tcataagggc gagcagaccg acagttgttg tttatttgca
     3901 acgggcaacc aggaaagtcg tccgccagcg cggacaatgc tgcgcagccg gaacggcaac
     3961 cgctgggcgg aaaggcgtgg ttaccggacg acgaggaccg gtatcttcga atgcgtcagg
     4021 actttagcgg tctcgctgcc gaggagcagg ctcgagagcc cccgtctgcc gtgcgacgcc
     4081 atcaggatga gatcgcactt cttcttcttc gcggtctcga tgatttgctg gtagggaaaa
     4141 tcgctgacga cggagacgga ttctcacttg acacccgcat cagcggcgag cttttcgacc
     4201 ttggcgagca ttttcctggc gtgcacctgg gattctttct cgaagcgctt tttgagctcg
     4261 gcgctcatcg gggatacgaa tccctcctcg accaccatac gaaactccgg caccacatgg
     4321 acggttgtca cacgcgcgtc gatctctttt gcaaactgaa tgccgcgctg gacggcccga
     4381 tccgacagtt tcgagccgtc ggtgggcagc agaatatgct tgaacatgga gcctccagtc
     4441 agtcaagcgt ctgtggcaag aataccacgc cgtccgaccc gggaatgagc cgccccagcc
     4501 ggctcgctgc tcagtgactc ttgccgagcc ggtgcaaggg aaccgggaga gccgctactt
     4561 cgataccttc ctcacgcagg gcttcgacat cctgagtgga tgcagtgcca cggatacgcc
     4621 gctcttccga ttcgccgtaa tggattttcc gagcctcttc tggaaacgcc gggcctacgt
     4681 cctcggtatt ctcgatgatg tgatcgatca gcttggcgag cacttctgcg cccaagttgg
     4741 cgtactgcct ctcctggggc gaggaaggct
//
LOCUS       DASURG010000280         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1172569, whole genome shotgun sequence.
ACCESSION   DASURG010000280 DASURG010000000
VERSION     DASURG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172569"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1442
                     /gene="radA"
                     /locus_tag="VFY80_04855"
     CDS             93..1442
                     /gene="radA"
                     /locus_tag="VFY80_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX6007267.1"
                     /translation="MAKPKSLYACTECGGQALKWQGQCPHCQAWNTLVETVAEAPPGN
                     RFGLVPGGKLQRLSEIEAREENRVPTGVPEFDRVLGGGLVPGAVVLIGGDPGIGKSTL
                     LLQALAEIGRSHKVIYVSGEESAQQIALRARRLGVDSGRVQVLPETDLKKIQHAIREE
                     RPDVAVIDSIQTLYSDELQSAPGSVAQVRECAAQLTRAAKASNATVVFVGHVTKEGTL
                     AGPRVLEHMVDTVLYFEGDTHSSFRLIRAFKNRYGAVNELGVFAMTDKGLKGVSNPSA
                     LFLSRHGGEVAGSCVTVTQEGTRPLLVEVQALVDEAHAPNPRRLSVGLEQNRLALLLA
                     VLHRHAGIACFDQDVFVNAVGGVRISEPAADLAVMMAIMSSLRNKPLPQKLVVFGEVG
                     LAGDVRPVQRGQERLKEAAKLGFEQALVPKANKPRHAISGIDVVAVERVEEAVARLR"
     gene            1520..>2991
                     /locus_tag="VFY80_04860"
     CDS             1520..>2991
                     /locus_tag="VFY80_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX6007268.1"
                     /translation="MARFNAYSAGLDRNPANYTPLTPLAFLTRSAYVYPRRVAVVYGE
                     RMISWADVYARSRRLASALAQRGVAEGDTVAVMLPNVPAMYEVHFGVPMTGAVLNTLN
                     TRLDAGAIAFMLQHAAAKVLITDPEFSSTIKPALEQLPAKPLVIDVDDPAIPGGTRLG
                     EIEYEAFIEQGDPEYEWAMPGDEWNAISLNYTSGTTGNPKGVVYHHRGAYLNAISNMV
                     SWGMPQHAVYLWTLPMFHCNGWCFPWTIAAASGTNICLRRIEARAVLDSIRRYRVTHY
                     CGAPIVHTMLLNAPAEWRAGIDHTVHCLVAGAAPPVAVIEGMERMGFDITHVYGLTET
                     YGPAAVCAKHEEWEALPLEKRAELNGRQGVWYHMQDRMAVIDPISMQPVPWDGETIGE
                     VMFRGNLTMKGYLKNPSATVEAFSGGWFHTGDLGVTQPDGYVKIKDRAKDIIISGGEN
                     ISSLEVEDALYRHPAVMAAAVVAQPDPKWGEVPCAFVELK"
BASE COUNT          534 a          969 c          991 g          497 t
ORIGIN      
        1 cgccgcgcgt tccggtcatc gagcgcacgt gagggtatgc gcggcgcgcg gtgtatgatc
       61 gtttcgccgt cgcagcttcc ataaacacca ccatggccaa acccaaatcg ctctacgcgt
      121 gcaccgaatg cggcggacag gccctcaagt ggcaagggca atgcccgcat tgccaggcgt
      181 ggaacacact ggtggaaacg gtggcggagg ccccgccggg caaccgcttc gggctcgtcc
      241 ccggcggcaa gctccagcga ctgtccgaaa tcgaagcgcg cgaggaaaac cgcgtgccca
      301 ccggcgtccc ggaattcgac cgcgttctgg gcggcggcct cgttccgggc gcggtcgtgc
      361 tgatcggcgg cgacccgggc atcggcaaat ccacgctgct cctgcaggcg ctcgcggaaa
      421 tcggccgctc gcacaaggtg atctacgtga gcggggaaga gtcggcgcag cagatcgcgc
      481 tccgtgcgcg ccggctcggc gtcgattccg gccgcgttca ggtgctgccc gagactgatc
      541 tcaagaagat ccagcacgca atacgcgagg agcggcccga cgtggccgtc atcgattcca
      601 tccagacgct ctattccgac gaactccagt ctgcaccggg ctcggtcgca caggttcgcg
      661 agtgcgcggc acaactcaca cgcgcggcca aggcttcgaa tgccacggtc gtcttcgtcg
      721 gccacgtcac caaagaaggc acgctggcgg gcccgcgcgt gctcgagcac atggtggaca
      781 cggtgctcta tttcgagggc gatacccact cgagcttcag gctgatccgg gcgttcaaga
      841 accgctatgg agcggtgaac gagctcgggg ttttcgcgat gacggacaag gggctgaagg
      901 gcgtctccaa tccttccgcg ctgttcctgt cgcgccacgg cggcgaagtg gcgggctcgt
      961 gcgtcacggt cacgcaggag ggcacgcgcc cgctgctggt cgaagtgcag gcgctggtcg
     1021 acgaagcgca cgcgccgaac ccgcgccgcc tctcagtcgg gctggagcag aaccggcttg
     1081 cactgctcct tgcggtactt caccggcacg ccggcatcgc gtgcttcgat caggacgtct
     1141 tcgtgaacgc ggtgggcggg gtgcgcatca gcgagcctgc ggccgacctc gccgtcatga
     1201 tggcgatcat gtcatcgctg cgcaacaagc cgctgcctca aaagctcgtg gtgttcggcg
     1261 aagtgggact ggcgggcgac gtgcgtcccg tgcagcgggg gcaggagcgg ctcaaggagg
     1321 cggcaaagct cggtttcgag caggccctcg ttccgaaggc gaacaagccg cggcacgcca
     1381 tttccggcat cgatgtggtt gccgtcgagc gcgtggaaga ggccgtcgcg cggctgcgct
     1441 gattggctgc ccgaacgacg cgcagctaga atggcgtgag ccgccgccac gcgcggcgga
     1501 atgcttcaaa ggagggttga tggctcggtt caacgcatac agcgccggcc tcgacaggaa
     1561 tcccgccaac tacacgccgc tcacgccgct tgcgttcctc acgcgcagcg cctatgtcta
     1621 tcccaggcgt gtggcggtgg tgtatggcga gcgcatgatc agctgggctg acgtctacgc
     1681 ccgcagccgc cggctcgcct cggcgctcgc ccagcgcggc gtcgcggaag gcgataccgt
     1741 cgccgtcatg ctgcccaacg ttccggcgat gtacgaggtc cacttcggtg tcccgatgac
     1801 gggcgcggtg ctcaacacgc tcaacacgcg tctcgatgcg ggcgcgattg cgttcatgct
     1861 gcagcacgcc gcggcgaaag tcctgatcac cgaccccgaa ttttcatcga ccatcaagcc
     1921 ggcgctcgag cagcttccgg cgaagccgct cgtgatcgac gtggacgatc ctgccattcc
     1981 aggcggcacg cgcctcggtg aaatcgaata cgaagcgttc atcgagcagg gcgaccccga
     2041 atacgaatgg gccatgccgg gcgacgagtg gaatgcgatt tcgctcaatt acacgtcggg
     2101 cacgaccgga aatccgaaag gggtcgtcta tcaccaccgc ggcgcgtacc tcaacgcgat
     2161 cagcaacatg gtctcatggg gcatgcccca gcatgcggtg tacctctgga cgcttcccat
     2221 gtttcattgc aacggctggt gcttcccgtg gacgatcgcc gcggcatccg gcaccaacat
     2281 ctgcctgcgc aggatcgaag cgcgcgccgt gctcgactcg atccggcgat atcgggttac
     2341 acattactgc ggtgcgccga tcgtccacac catgctgctc aacgcccctg ccgaatggcg
     2401 tgccgggatc gatcacacgg tccattgcct cgtggccggc gcggcgccgc cggtcgcggt
     2461 catcgaaggc atggagcgta tgggcttcga catcacgcac gtctacggac tcaccgaaac
     2521 ctacggcccg gccgccgtat gcgccaagca cgaggagtgg gaggcgttgc cgctggagaa
     2581 gcgcgccgag ctgaacggcc gccaaggggt gtggtaccac atgcaggatc gcatggcggt
     2641 catcgatccc atctcgatgc agcccgtgcc gtgggacggc gagacgatcg gggaggtcat
     2701 gttccgcggc aatctcacca tgaagggtta tctgaaaaac ccgtctgcga ccgtggaagc
     2761 attctcgggc ggctggtttc acaccggcga tctcggcgtg acgcagcccg acggctacgt
     2821 gaagatcaag gatcgtgcga aggacatcat catctcgggt ggcgaaaaca tttcctcgct
     2881 cgaagtcgag gacgcgctct accgccatcc ggcggtgatg gcggccgcgg tggtcgcgca
     2941 gcccgatccg aagtgggggg aagtcccgtg cgccttcgtc gaattgaaag a
//
LOCUS       DASURG010000281         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1172636, whole genome shotgun sequence.
ACCESSION   DASURG010000281 DASURG010000000
VERSION     DASURG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172636"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..2189)
                     /locus_tag="VFY80_04865"
     CDS             complement(87..2189)
                     /locus_tag="VFY80_04865"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6007269.1"
                     /translation="MKLIRFLPTVLMLAVMPAAAGAQSNQQLAQQVTIRRTEYGVPHL
                     LADNYKALGFGLGYAQVEDYGARVVNGLMGVRGQRGLIFGRDSIDDDFLNRLAHRRTV
                     ATYHLIDPDVRQVLEGFAAGVNYYVELHRSEFPGWLQANFTGHDVAAIAFGGGTSVSA
                     ALRLIRRQSEPPNAAAAAGNEGSNAWAFAPSRTKSGRAILLRNPHLNWNAGYWEAHVT
                     IPGELNFYGDFRLGGPFTIIGGFNEHLGWATTNNNVDTDEVYALAVDPDRPDHYLFDG
                     ISIPLQRELLTAEYRNGPGINTMTREFWTTPLGPVVDRVNGKIYVLRAAGEGEYRNGD
                     QFLYMMRARNLEEWKQAMRIRSRTTSNFTYADRAGNIFYIWNGSQPDFPPNSGGDSIP
                     VPAHTSAEVWRQLVPFDRLPQLHNPKGGYIHQENDPFHYTNLNAVFDSTLYPPPFPRP
                     QLRLRSQHALQLVHGSKKVSLEDVVAMKHSYRMLLADRVKPALLAALAAAQLDADARG
                     AAEVLRRWDNRAAHDSRGALLFQTWWQRYAMRLPQDSLYEEVWTPKSPTTTPRGLKNP
                     QRAVEAFLWAVPETRRLYGAIDAPWGEFMRVRRGSVDVPVGGCSGAMGCFRVLNFEQQ
                     PDGKYAVNGGDGWVLAVEFGNVPRAYSVLGYGQSSRPESPHFSDQAAMFAAGQMKKVP
                     FTEADIRKTTIRTYHPGQ"
     gene            complement(2233..2544)
                     /locus_tag="VFY80_04870"
     CDS             complement(2233..2544)
                     /locus_tag="VFY80_04870"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEX6007270.1"
                     /translation="MNALALGPQDRGREVPVRVGDRIVLRLPENPTTGFRWAGDIPAF
                     LQVERDANESSAAPGAGGMRILELSVRTAGRAELRLVCGRSWDPSAPPTDRFNVMLQA
                     Q"
     gene            complement(2541..2969)
                     /locus_tag="VFY80_04875"
     CDS             complement(2541..2969)
                     /locus_tag="VFY80_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007271.1"
                     /translation="MARTTEKPGTPKELPPYDVETLTALPAVGQDGPTLSVEELSARV
                     LEQGAVPVLTPPAEIRDAGMAQGITAWHNSVKITALWCNASARNAWAAIPGLGWRRVV
                     FGNDSAFLNITALLSQARQNNSTCNIRIEADNLIHEVYVW"
     gene            complement(2998..>3286)
                     /locus_tag="VFY80_04880"
     CDS             complement(2998..>3286)
                     /locus_tag="VFY80_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007272.1"
                     /translation="IAYGECQIETWSGPWGANAVTLRNWRNNVLINGLWHNESDRNAW
                     VHVAGVGWRKLATETASMQSAMLLELAAAKGANRPVNILEDQQTVREVYVL"
BASE COUNT          592 a         1050 c         1045 g          599 t
ORIGIN      
        1 catgtctggg tgaatggtgt ccaggtcgtg cgtgatggtg tgcacaccga cgcacgaccc
       61 ggccgcgtgc tcaggtcagc gccgcgtcac tgaccggggt gatacgtgcg gatcgtcgtc
      121 ttgcggatgt ccgcctctgt gaatggcact ttcttcatct gacccgcggc gaacatggcg
      181 gcctgatcac tgaagtgtgg cgattcgggc ctgctgctct gcccgtaacc cagcaccgag
      241 taggcacgcg gcacgttgcc gaattcgacg gcgagcaccc agccatcgcc gccgttcacg
      301 gcgtacttgc catcgggctg ctgttcgaag ttgagcacac ggaagcagcc catggcgccc
      361 gagcagccac cgaccggcac atcgaccgag ccacgccgca cgcgcatgaa ctcaccccac
      421 ggtgcgtcga tagcgccgta cagtcgcctg gtttccggca cagcccagag gaacgcctcc
      481 actgcgcgct gcggattctt caatccgcgc ggtgtcgttg tgggtgattt cggcgtccat
      541 acttcttcgt agagcgaatc ctgcggcagg cgcatggcgt agcgctgcca ccacgtctgg
      601 aagaggagtg cgccccgcga atcgtgcgcg gcccgattgt cccagcgacg cagcacctcc
      661 gcggccccac gtgcatccgc atccagctgg gcggcagcca gtgcggcgag caatgccggc
      721 ttcacacgat ctgccaacag catgcgatag ctgtgcttca tggccacgac gtcttccagc
      781 gacaccttct tgctgccgtg cacgagctgc aatgcgtgct gactgcgcag gcgcagctgc
      841 ggacgcggga agggcggcgg atacaacgtc gaatcaaaga ccgcgttcag attggtgtag
      901 tggaacggat cgttttcctg gtggatgtag ccgcctttcg gattgtgcag ctgcggcagc
      961 cgatcgaagg gcacgagctg ccgccagacc tccgcggatg tatgggccgg cacgggaatc
     1021 gaatcgccgc cgctgttcgg tggaaaatcc ggctgcgagc cattccagat gtagaagatg
     1081 ttaccggcgc gatcggcata cgtgaagttc gacgtcgtgc gcgaacggat gcgcatggcc
     1141 tgcttccatt cttccagatt gcgtgcgcgc atcatgtaca ggaactgatc accgttgcga
     1201 tactcgcctt caccggccgc gcgcaacacg tagatcttgc cgttgacgcg atcgacgacg
     1261 gggccgagcg gcgtcgtcca gaactcgcgc gtcatggtgt tgatgcccgg gccgttgcgg
     1321 tattcggccg tcagcagctc gcgctgcaat ggaatggaga tgccgtcgaa gagataatgg
     1381 tcgggcctgt ccggatcgac agcgagcgcg tacacctcgt ccgtgtcgac attgttgttg
     1441 gtcgtggccc agccgagatg ctcgttgaag ccgccgatga tggtgaaggg tccgcccagg
     1501 cggaaatcac cgtagaagtt gagctcgccg ggaatggtga catgcgcttc ccagtacccg
     1561 gcattccaat tgagatgcgg attgcgcagc agaatcgcgc gccccgactt cgtgcgcgaa
     1621 ggcgcgaacg cccaggcatt ggatccctca ttgcccgcgg cggcggccgc attgggcggc
     1681 tccgactgcc gccggatcag acgcagcgcg gcactcaccg acgtaccgcc gccgaacgct
     1741 atggctgcca catcatggcc ggtgaagttg gcctggagcc agccgggaaa ttcggagcgg
     1801 tgcagctcga cgtagtagtt gacgccggcc gcgaagccct cgagcacctg gcgcacgtcc
     1861 gggtcgatca ggtggtacgt agccaccgtg cggcggtggg cgagccgatt gaggaaatcg
     1921 tcgtcgatgc tgtcacggcc gaagatcagg cctcgctggc cgcgcacacc catgaggccg
     1981 ttgacgacgc gcgcgccgta gtcctcgacc tgcgcgtagc ccaggccgaa gccgagcgcc
     2041 ttgtaattgt cggccagcag gtgtggcaca ccatactcgg tgcggcgaat ggtcacttgc
     2101 tgtgcgagct gctgattgga ttgcgcgccg gccgcggccg gcatgaccgc cagcatcagc
     2161 actgtcggaa ggaagcgaat cagtttcatg gagacggatg ctacggctgc acgccgggca
     2221 tcgccagact tgctactgcg cttgcagcat cacattgaag cgatccgtgg gcggcgcgga
     2281 tgggtcccac gatcgtccgc aaaccagccg cagctcggcg cgacccgcgg tacgcaccga
     2341 caattcgaga atgcgcatac caccggcacc gggcgcagcg gacgactcgt tggcatcccg
     2401 ctcgacctgc agaaacgcag gaatgtcgcc cgcccagcgg aaaccggtgg tgggattctc
     2461 cggcaaccgg agcacgatgc ggtcaccgac acgcactggg acctcgcgac cgcgatcctg
     2521 aggccccagt gccagggcat tcaccagacg tacacctcgt gaataaggtt gtccgcttca
     2581 attcgaatat tacaggtcga attgttctgc cgcgcctggc tgagcaaggc ggtgatgttg
     2641 aggaatgcgc tgtcgttgcc gaatacgacg cgccgccagc cgagccccgg gatggcggcc
     2701 caggcgttac gcgccgacgc gttgcaccac aacgccgtga tcttcacact gttgtgccag
     2761 gctgtgatgc cctgcgccat gccggcgtca cgaatttctg cgggcggcgt cagcaccggc
     2821 acggcgccct gttcgagaac gcgcgcactg agctcttcga ccgacaaggt cgggccatcc
     2881 tgacccaccg caggcaaggc ggtcagcgtc tcgacgtcgt acggaggcag ctctttgggc
     2941 gtccccggtt tttcagttgt acgagccatt ggttacctcg catgtgaaag tcatgcacta
     3001 cagcacgtag acctcacgca ccgtttgctg atcttcgaga atattgaccg gacgatttgc
     3061 gcccttggcc gcggccagct ccagcagcat ggcgctctgc atggaagccg tttcagtggc
     3121 gagcttgcgc cagcccacgc ccgccacgtg cacccaggca ttgcgatccg attcgttgtg
     3181 ccacaggccg ttgatgagca cgttgtttct ccagttgcgc agcgtcaccg cattcgcacc
     3241 ccacggtccg gaccatgtct cgatctggca ctcgccatac gcaatg
//
LOCUS       DASURG010000282         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_49237, whole genome shotgun sequence.
ACCESSION   DASURG010000282 DASURG010000000
VERSION     DASURG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49237"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VFY80_04885"
     CDS             complement(<1..1143)
                     /locus_tag="VFY80_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6007273.1"
                     /translation="MDFALNAEQIAFQDSVRAFAKRELEAQALERAHSEAYPREVAKR
                     MAGAGLLGITIPEADGGQGGTLMDAVLAIEQVALVCPRSADIVQEGNFGAIRVLARFA
                     SEDQKKRFLTPLLAGDEIIAVAMTEPDAGSATTDLATAAKSDGAGFRVTGTKVFPNPH
                     ADQYLVYVRFGPRVEGIGSVMIRRGTPGFTIGQASRFMSGAHWAPLYFDHVYVPPEDV
                     LLGPGGFKQQMAGFNAERVGNAARSLAFGRYCFNVARDYALTRRQFGRPLCEFQGLQW
                     KFADMKVKLDAAQLLLYRAAANADRGFPDAEETTVAKLACNQAGFDVANEALQVMGGL
                     GYTQETLVEYCVRRTRGWMIAGGSIEMMKNRLAESIFGRRFSQRPPR"
     gene            complement(1153..>2033)
                     /locus_tag="VFY80_04890"
     CDS             complement(1153..>2033)
                     /locus_tag="VFY80_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6007274.1"
                     /translation="FLMKVMCFALFACAFNLLLGFAGLLSFGHAMFLGMAGYSAAHAA
                     KVWGLNPEFAILLGTAASCLLGVVSGMIAIRRQGIYFAMITLALAQMIYFFCVQAPFT
                     HGEDGIQAVPRGKLFGVIDLADVRAMYVLVFVIFIAGFLLIYRIIHSPFGQVLKAIRE
                     NEPRAVSLGYDTDRYKLLAFVLSATLSGLAGATNALVFQLAALNAVYWTMSGEVVLMT
                     LVGGMGTIFGPVVGALVVLAMENYLAQLGAWVTIVQGLVFIVCVLTFRRGIVGEWVHF
                     LRRREAARSKRSATAD"
BASE COUNT          417 a          673 c          625 g          318 t
ORIGIN      
        1 gcgaggcggg cgctgggaga agcggcgccc gaaaatcgac tccgcaagtc ggtttttcat
       61 catctcgatc gaaccgccgg cgatcatcca gccgcgcgtg cggcgcacgc agtactcgac
      121 gagcgtctcc tgggtgtaac cgagcccgcc catcacctgc agcgcctcgt tggcgacatc
      181 gaaccctgcc tggttgcagg cgagcttggc gaccgtcgtt tcttccgcgt cgggaaagcc
      241 gcggtcagcg tttgccgcgg cgcgatagag gagcagttga gccgcgtcga gctttacctt
      301 catgtccgcg aatttccact gcagcccctg gaactcgcac agcgggcgcc cgaactgtct
      361 gcgggtgagc gcgtagtcgc gcgcgacgtt gaagcaatag cgcccgaatg cgagcgagcg
      421 agcggcattg cctacgcgct cggcgttgaa acccgccatc tgctgcttga agccgcccgg
      481 tccgagcagg acgtcctcgg gcggaacgta gacgtggtcg aaatagagcg gcgcccagtg
      541 cgcgcccgac atgaaccggg acgcctggcc gatggtaaat cccggcgtgc cacggcggat
      601 catcaccgag ccgatgccct cgacacgcgg gccgaagcgc acatagacga ggtactgatc
      661 ggcatgcggg ttgggaaaga ctttcgtgcc cgtcacgcgg aagcccgcgc catcggactt
      721 cgcggcggta gcgagatccg tcgttgccga gcccgcgtca ggctccgtca tcgcgacggc
      781 gatgatttcg tctccggcaa gaagcggcgt gagaaagcgc ttcttctggt cttcgctcgc
      841 gaaccgcgca agcacgcgta tggcgccgaa gttcccttcc tgcacgatgt ccgcgctccg
      901 cgggcacacg agcgccacct gctcgatcgc gagcaccgcg tccatgagcg tcccgccctg
      961 cccaccgtcc gcctcgggaa tcgtgatccc gaggagcccg gccccggcca tacgtttcgc
     1021 cacttcccgc ggataggctt cagaatgtgc gcgctcgagc gcttgagcct ccagttcacg
     1081 cttggcgaag gcgcgtaccg aatcctgaaa cgcgatctgc tcggcattca gcgcaaagtc
     1141 catctccgca catcagtccg cggtcgcgga gcgcttggaa cgcgccgctt cccgccttcg
     1201 gaggaagtgc acccattcac caacgattcc gcggcggaaa gtcaacacac atacgatgaa
     1261 aaccaaaccc tgaacgatcg tcacccaggc accgagctgc gcaagatagt tttccatggc
     1321 gagcacgaca agcgcgccca cgaccggccc gaatatcgtg cccatgccgc ccacgagcgt
     1381 catcaacacg acttctccgg acatggtcca gtacacggca ttgagcgcgg caagctggaa
     1441 gacgagcgca ttggtcgccc ccgcgagtcc ggaaagcgtc gcggacagca cgaacgcaag
     1501 cagcttgtaa cgatcggtgt cgtagccgag cgagactgcg cgcggctcgt tctcgcggat
     1561 cgctttcagg acctggccga acggcgagtg gatgatgcgg tagatcagga gaaagcccgc
     1621 gatgaagatg acgaacacca gcacgtacat cgcgcgcaca tccgcgagat cgatgactcc
     1681 gaagagcttg ccgcgcggca cggcctggat gccgtcctcg ccgtgcgtga agggcgcctg
     1741 cacgcaaaag aaatagatca tctgcgccag cgcgagcgtg atcatcgcga agtagatccc
     1801 ctgcctgcgg atcgcgatca ttcccgacac cacgccgagc aagcaggatg ctgcggttcc
     1861 gagcagaatc gcaaactcgg gattgagccc ccacaccttc gctgcatgcg ccgcggaata
     1921 accggccatg ccgaggaaca tcgcgtggcc gaaggacagc aaaccggcaa aaccgaggag
     1981 aagattgaac gcgcacgcga agagtgcgaa gcacatcacc ttcatcagga aca
//
LOCUS       DASURG010000283         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_968807, whole genome shotgun sequence.
ACCESSION   DASURG010000283 DASURG010000000
VERSION     DASURG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968807"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..191
                     /gene="rpmD"
                     /locus_tag="VFY80_04895"
     CDS             9..191
                     /gene="rpmD"
                     /locus_tag="VFY80_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX6007275.1"
                     /translation="MKITQIRSANGRPEVHRRTLRALGLKHQRSVIHEENEAIKGMLF
                     QVRHLVEVSEVEGEEK"
     gene            192..644
                     /gene="rplO"
                     /locus_tag="VFY80_04900"
     CDS             192..644
                     /gene="rplO"
                     /locus_tag="VFY80_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX6007276.1"
                     /translation="MSDLHNLRRPEGAHRGKKRLGRGPGSGKGKTSGKGHKGQKARTG
                     HHGIPSWFEGGQMPLQRRVPKRGFKNPFRTVYEIVNVGQLSKLDEDTITPETLHSARL
                     VDVGRGNPVKVLGDGEVTRKLTVRAHAFSESARAKIEAAGGTVELLEA"
     gene            647..1969
                     /gene="secY"
                     /locus_tag="VFY80_04905"
     CDS             647..1969
                     /gene="secY"
                     /locus_tag="VFY80_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6007277.1"
                     /translation="MANPIPNLFKIPELKEKILFTLMTLLIYRIGAHITAPGIDVGAL
                     REQFGQLQNSLFGVYDMFVGGALSRATIFGLGIMPYISASIMFQLLAAVVPSIEKMQK
                     EGEDGRKKLTQWTRYTTVGLAFLQGYGYAVFVQSIPGAVRNPGIFFLLSSALILTIGA
                     VFIMWLGEQITERGIGNGMSLLIFLSIIEGFPTQVATTWEQVATDVISPWAVLLMLVI
                     MVGVVAGVVAMTMAARKIPVQIPRKVMGRGRVREGQKSFIPLRINSSGVMPIIFAQSI
                     IIVPGTVAAFMGQNQTGIMGEITRALSPGSWIYYSLYAVLILFFSYFYTAIIFNPVDL
                     AENLKKQGAFIPGVKPGARTAEHIDFVLSRITLAGAIYLALIALLPFWVAGVFNMQNF
                     FFGGTSLLIVVGVALDTVQQMQQHMLLRHYEGFMKKGRVRFRGRQRFM"
     gene            2125..2766
                     /locus_tag="VFY80_04910"
     CDS             2125..2766
                     /locus_tag="VFY80_04910"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX6007278.1"
                     /translation="MNLVLFGPPGAGKGTQGTVLAETRGLMKISTGDILRDAVRAGTE
                     LGLQAKKFMDAGELVPDSVILGLVREAIQDAGSGFIMDGFPRTIAQAEGVDAMLAEMG
                     KTIDAVIILQVPDETLVQRLSGRRSCPNCGAVYNVFFEKPKNDEKCDRCDSELVQRAD
                     DKRDTVVRRLEVYKQQTAPIVDYYKRRGAKVKFVDGDRPVDQVQRDILRIIDQ"
     gene            2763..3509
                     /gene="map"
                     /locus_tag="VFY80_04915"
     CDS             2763..3509
                     /gene="map"
                     /locus_tag="VFY80_04915"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX6007279.1"
                     /translation="MIALKTEDQIAAIGRAGAILSALFEAIKPEIRPGVSTADLDLFG
                     ENFIMSYPGAVPSFKGLYGFPASLCISINHEVVHGIPSAKRILQEGDIVSIDAGVQLD
                     GWFADAARTYAVGDVDAVSEVLLRVTQEALDAGIAHARAGNRLGDIGAAVQNVAETAG
                     FSVVRDLVGHGIGRNPHEEPQVPNFGKAGKGLILQPGLVLAIEPMVNAGTPAVRTLAD
                     KWTVVTLDKKRSAHFEHTVAITADGPKVLT"
     gene            3587..3703
                     /gene="rpmJ"
                     /locus_tag="VFY80_04920"
     CDS             3587..3703
                     /gene="rpmJ"
                     /locus_tag="VFY80_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX6007280.1"
                     /translation="MKVRSSVKPICEHCKVIKRAGVVRIICKKNPRHKQRQG"
     gene            3709..4089
                     /gene="rpsM"
                     /locus_tag="VFY80_04925"
     CDS             3709..4089
                     /gene="rpsM"
                     /locus_tag="VFY80_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX6007281.1"
                     /translation="MARIAGVDLPRNKRIEIGLTYIYGIGQATSNKILETAGVNPDRR
                     VHELNDDEVNRLRRIIESQFKVEGALRTEVSMNIKRLMDIGAYRGLRHRKGLPVRGQR
                     THTNARTLKGKRVAIAGKKKATKK"
     gene            4163..>4389
                     /gene="rpsK"
                     /locus_tag="VFY80_04930"
     CDS             4163..>4389
                     /gene="rpsK"
                     /locus_tag="VFY80_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6007282.1"
                     /translation="MADPKKKGGPRTKRARKHVDAEGVAHIKATFNNTLVSITDMAGN
                     SVVWGSAGKAGFKGSKKSTPFAATVAAEQAAR"
BASE COUNT          900 a         1237 c         1408 g          844 t
ORIGIN      
        1 cgcgccgctt gaagatcacg cagatccgca gtgccaatgg ccggcctgaa gtgcatcgca
       61 gaacgttgcg tgcgctcggc ctgaagcatc agcggtctgt tattcatgaa gagaacgaag
      121 ccatcaaggg catgctgttc caggttcgcc atctcgtcga ggtgagcgaa gtggaaggtg
      181 aggaaaagta aatgtcggac ttgcataatc tccgtcgccc ggaaggcgcg catcggggca
      241 agaagcgtct gggacgcggc cccggttcgg gcaagggcaa gacgtcgggg aaaggtcaca
      301 agggacagaa ggcccgtacc ggtcaccacg gcattccgtc atggttcgaa ggcggccaga
      361 tgccgttgca gcggcgcgtg ccgaagcgtg gcttcaagaa cccgttccgc acggtttacg
      421 agatcgtgaa cgtcggacag ctgagcaagc tggacgaaga cacgattacg ccggaaacac
      481 tgcactcggc acgcctggtc gacgttggcc gcggcaatcc ggtgaaggtg ttgggcgatg
      541 gcgaagtgac gcgcaagctg acggtgcgcg cacacgcctt cagtgaaagt gcacgggcca
      601 agatcgaagc ggcgggcggt acggtcgaac tgctggaggc ctgatcatgg ccaatccgat
      661 cccgaatctg ttcaagatcc cggagctgaa agagaaaatt ctcttcacgc tcatgacgtt
      721 gctgatctac cggatcggtg cgcacatcac tgcgcccggc atcgacgtgg gcgcgctgcg
      781 tgagcagttc ggccagctgc aaaactcgct gtttggtgtc tacgacatgt tcgtcggtgg
      841 tgcgctttcc cgcgcgacca tctttggcct gggcatcatg ccgtatatct cggccagcat
      901 catgttccag ctgctggccg cggtggtgcc ttccatcgag aagatgcaga aggaaggcga
      961 ggacggccgc aagaagctga cgcagtggac ccgttacacc accgttggtc tggccttcct
     1021 gcagggttac ggctacgccg tcttcgtgca atcgattccc ggcgcggtgc gcaatccggg
     1081 tatcttcttc ctgctttcgt cggctttgat tctgaccatc ggcgccgtct tcatcatgtg
     1141 gctgggtgag cagatcaccg agcgcggcat cggcaacggt atgtcgctgc tgatcttctt
     1201 atcgatcatt gaaggcttcc cgacgcaggt tgccaccacg tgggagcagg ttgccaccga
     1261 cgtcatttcg ccgtgggcag tgttgttgat gctggtgatc atggtgggtg tggtggccgg
     1321 cgttgtggcc atgaccatgg cggcgcgcaa aatcccggtg cagatcccac gcaaggtgat
     1381 gggacgcgga cgcgtacgtg aaggtcagaa gagcttcatt ccgctgcgca tcaactcgtc
     1441 gggcgtgatg ccgatcatct tcgcgcagtc gatcatcatc gtgccgggca cggtggccgc
     1501 gttcatgggc cagaaccaga ccggcatcat gggcgagatc acgcgtgcac tcagcccggg
     1561 cagctggatc tactattcgt tgtacgcggt gttgatcctg ttcttctcgt acttctacac
     1621 ggccatcatt ttcaacccgg tggacctggc cgagaacctg aagaagcagg gcgcattcat
     1681 tcccggtgtg aagcccggtg cgcgcacggc cgaacacatc gatttcgtgc tcagccgtat
     1741 tacgctggcc ggtgcgattt acctggcgtt gattgcgctg ctgcctttct gggtggcggg
     1801 cgtgttcaac atgcagaact tcttcttcgg cggtacttcg ctgctgatcg tggttggtgt
     1861 ggcgctggat accgtgcagc agatgcagca gcacatgctg ctgcggcatt acgaaggttt
     1921 catgaagaaa ggccgcgtgc gcttccgcgg acgccagcgc ttcatgtaac gtcgcatcgc
     1981 cggcgcttac gatcgtgcac gcacacgaac acgtgcacgg tcacgttgaa ggcgcgaagc
     2041 catagatacg gcctcgcgcc ttttcattcg attttcagtc gtgtacgtgg tccgtgttcg
     2101 tgcacgagac cgatccataa gggcatgaat ctagttctct tcggtccgcc aggggccggc
     2161 aaaggcacgc aaggcactgt tctcgcggaa acgcgtggac tgatgaaaat ctcgacgggt
     2221 gacatcctgc gcgatgcggt gcgcgccggc acagagctcg gcttgcaggc aaaaaaattc
     2281 atggatgccg gcgagctcgt gcctgactcc gtaattctcg gcctggtgcg tgaggcaatc
     2341 caggacgcgg gcagcggttt tatcatggac gggttccctc gcaccatcgc gcaggccgaa
     2401 ggtgtcgacg ccatgctcgc ggagatgggc aaaaccatcg acgccgtgat cattctgcaa
     2461 gtgcccgatg agacgctcgt gcagcggctg agcggacgac gctcgtgtcc caactgcggc
     2521 gcggtctaca acgtgttctt cgagaaaccg aaaaacgatg agaagtgtga ccgctgcgac
     2581 agcgagctgg tgcagcgggc cgatgacaaa cgcgacaccg tggtgcggcg gctcgaagta
     2641 tacaaacaac agactgcacc tatcgtggat tactacaaac ggcgcggcgc gaaagtgaag
     2701 ttcgtcgacg gtgatcgccc cgtcgaccag gtccagcgtg acattctgcg gatcatcgac
     2761 cagtgattgc gctcaaaacc gaggaccaga tcgcggcaat cggacgggcc ggcgctatcc
     2821 tgtcggctct tttcgaagcc atcaagccgg agatccggcc cggtgtgagc accgccgacc
     2881 tcgacctgtt cggcgaaaac ttcatcatgt cgtatccggg cgcagtgccg tccttcaaag
     2941 ggctgtacgg cttcccggcc agcctgtgca tctcgatcaa ccatgaggtg gtgcatggca
     3001 tcccgagtgc aaaacggatc ctgcaggagg gcgacatcgt cagtatcgac gccggcgtcc
     3061 agctggatgg ctggttcgcc gacgccgccc ggacctacgc cgtgggagac gtcgatgccg
     3121 tgtctgaagt cctgctgcgc gtgacgcagg aggcgctgga tgccggtatc gcccatgccc
     3181 gcgccggcaa ccgcctcggc gacatcgggg cggcggtcca gaatgtcgcg gaaacggccg
     3241 gtttttcggt ggtccgcgac ctggttggcc atggcattgg ccgcaacccg catgaggagc
     3301 cgcaggtccc caatttcggc aaggccggca agggactgat cctgcagccg ggtctggtgc
     3361 tggcgatcga gcccatggtt aatgcgggca cgcccgccgt acgcactttg gcggacaaat
     3421 ggactgtagt aacgctggac aagaagcgca gcgcccattt cgagcacacc gtcgcgatca
     3481 cggcggatgg gccgaaagtc ttgacataaa agtggttcgg gcccttgaga caaggcccgg
     3541 ccgttcatat atttaaaagc tgttctataa ccgtatttgg tgccccatga aagtgcgtag
     3601 cagcgtcaag ccgatttgtg agcactgcaa ggtgatcaag cgggccggag tggttcgcat
     3661 catctgcaag aagaacccgc gtcacaagca gcggcaagga taaggaatat ggctcgtatt
     3721 gctggagtcg atctcccgcg caacaagcgc atcgagattg gtctgaccta catctacggc
     3781 attggtcagg cgacatcgaa caagattttg gagacggcgg gggtgaatcc cgatcgtcgc
     3841 gttcacgagc tgaatgacga cgaggtgaac cgtctacgtc gcatcatcga aagccagttc
     3901 aaggtggaag gcgcgctgcg cacggaagta tccatgaaca tcaagcgatt gatggacatt
     3961 ggtgcttacc gcgggctgcg tcaccgcaag ggtttgccgg tgcgcggaca gcgcacgcat
     4021 accaacgcgc gcacgttgaa gggtaagcgt gtggcgattg ccggtaagaa gaaagcgacg
     4081 aagaaataga gccgcctgat ggtggcaagc ccacgagctg gagctcgtcg gcccttaact
     4141 aaaaatgcgg cgataagaat ttatggcaga tccgaagaag aagggcggac cgcgtaccaa
     4201 gcgtgcgcgt aagcatgtcg atgccgaggg cgtcgcgcat atcaaggcga ccttcaacaa
     4261 cacgctggtg agcatcaccg acatggccgg caactcggtc gtgtggggga gcgccggcaa
     4321 ggcggggttc aagggatcca agaagagcac cccgttcgcc gcgacggtgg cggcggagca
     4381 ggccgcccg
//
LOCUS       DASURG010000284         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1480843, whole genome shotgun sequence.
ACCESSION   DASURG010000284 DASURG010000000
VERSION     DASURG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480843"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1074
                     /locus_tag="VFY80_04935"
     CDS             103..1074
                     /locus_tag="VFY80_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007283.1"
                     /translation="MKAPTWAWAAFAAAAIAGSLPAGAQQWPAKNVRILVPFPPGGGT
                     DIQARILSTAFQKSTGQNFIVDNRTGASGLIAAQLAVESPPDGYTILFTSGSISVVAT
                     LRAKQMKFNIYEDLVPVSLISSTPLVLSLHPSVPAKSVKQLVALAKRTSTPLNAGGNT
                     SGSTAHLTAEMFNQAVGIKSTVVTYRGGGPAVVALISGEIDYIFATAPSIMPHVRSGK
                     AKALAVTTPKRSSALPDLPTMNSIYPGFESWNWYAMFYPKGTPQPIIDKMNSEIRKAL
                     ESSEVKDFYPKQALDAAGSSPEELSKLLRSEIAKYAKVIKAAGIRTN"
     gene            complement(1153..1689)
                     /locus_tag="VFY80_04940"
     CDS             complement(1153..1689)
                     /locus_tag="VFY80_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007284.1"
                     /translation="MESSYNIPGALFSRFAASRSMRAFATMSAAAVTLTGCYAIPVGP
                     GPDGTPYYAYSPVPLAPVPRTNGSLPRIAPAGPMPASLPVKLYPSNDLANQTGVLTGE
                     VTNLMTGKGRFAFNYQGEMLVGEATRVANDERRGVAAAYGPRGTFARCEYQMSTPLMG
                     AGTCAFSNGAQYQVHIGS"
     gene            complement(1887..>2309)
                     /locus_tag="VFY80_04945"
     CDS             complement(1887..>2309)
                     /locus_tag="VFY80_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6007285.1"
                     /translation="LRSIGTQFVERFDLQRGLSDTERLLELIHNGEPLGFFPEGTFRR
                     MPGLLPFRMGAFVIAAQAGVPVLPVALRGTRSILREGHWLPRSGSLSVTIGPPLVPRA
                     GDWNAAIELRDAARQGILLRVREPDLALETVGTKAPTS"
BASE COUNT          445 a          709 c          732 g          423 t
ORIGIN      
        1 cactgggcgc gactagtcca taatttggat cacacgccgc tttgctgcta gtatgggtcg
       61 cgcggccatc gtagccgcca caccaaatga ggaggaggtt ccatgaaagc cccgacttgg
      121 gcatgggctg cctttgctgc agccgcgatt gccggctctc tgcccgccgg cgctcagcaa
      181 tggcccgcaa agaacgtgcg catcctcgtg ccgtttccgc ccggtggcgg gaccgacatt
      241 caggcgcgca tcctttccac cgcgtttcag aaaagcaccg gccagaactt catcgtggac
      301 aaccgtaccg gagcgagcgg tctcatcgct gcacagctcg ccgtcgaatc gcctccggac
      361 ggctacacga tattgttcac ctccggcagc atctcggtgg tggcgacgct gcgcgcgaag
      421 cagatgaaat tcaacatcta cgaggacctc gtgccggtga gcctcatttc gtccacgcct
      481 ctggtgctgt cgcttcatcc gagcgtgccg gcgaagtcgg tgaagcaact tgtcgcgctg
      541 gcgaagcgca catccacgcc gctcaatgca ggcggaaata cctcaggcag caccgcgcat
      601 ctcaccgctg aaatgttcaa tcaggcagtg gggatcaaat cgacggtggt cacttaccgc
      661 ggcggcgggc cggcagtggt cgcgctgata tccggggaga tcgactacat cttcgctacc
      721 gctccctcga tcatgcccca tgtgagaagc ggcaaagcga aggcgctcgc cgttacgacg
      781 cccaaacgct catctgcgct tcccgatctt ccgacgatga attcgatcta tccaggcttc
      841 gagtcgtgga actggtacgc catgttctat ccgaagggca caccgcagcc gatcatcgac
      901 aagatgaact cggaaattcg caaggcgctc gagagcagcg aggtcaagga tttctatccc
      961 aagcaggcgc tcgacgcggc aggaagttcg cccgaggaat tgagcaagct cctgaggagc
     1021 gagattgcaa agtacgccaa agtgatcaag gcggccggaa tccggacaaa ctgaccggcg
     1081 cccgtggttt cggcgccgct tcgccgggga ggcacaccgg cggagcggcg caactaaacg
     1141 ctgctgtgat tgtcagctgc cgatgtgcac ctggtactgg gcgccgttcg agaaggcgca
     1201 cgttccggcc cccatgagag gcgtgctcat ctggtactcg cagcgcgcga acgtgccgcg
     1261 tggaccgtac gcggctgcga cgccgcgacg ttcatcgttg gccacgcgcg ttgcctcacc
     1321 caccaacatc tcgccctggt aattgaaagc gaaacgcccc ttgccggtca tgagattcgt
     1381 cacctcgccg gtgagtaccc cggtctgatt ggcgagatcg ttgctcggat agagttttac
     1441 cggcaggctt gcgggcatcg ggcccgcggg tgcaatgcgt ggcagactgc cgttggtgcg
     1501 tggaaccggc gcgagtggca ccggggaata ggcgtagtaa ggcgtgccgt ccggacccgg
     1561 accgacgggt atcgcataac atcccgtgag tgtgacggct gcggctgaca ttgtggcgaa
     1621 tgcgcgcatg gaacggctcg cggcaaagcg actgaaaagc gcgcccggta tgttgtacga
     1681 agattccatc tcactctcct gtaggatttt tttcgcgcgc tgcctcggca cggtccaggc
     1741 cgctggtcgt ggcgcggcct gcaagccgaa tatcgtgcac gcgatgccgg cattctcgtc
     1801 gcccgcggct ttcgactggg ttaccgacag gttaccgatg gcttacaggc gggatgccgt
     1861 acgcaagtca cggaagcgct gcgattttat gatgtaggtg ctttggtgcc gaccgtttcg
     1921 agggcgagat ccggctcccg cacgcggagc agaattccct ggcgggcagc gtcgcgcagc
     1981 tcgatggcgg cgttccagtc gcctgctctc ggcacaaggg gcggtccgat cgtcacgctt
     2041 agggacccgc ttcgcggcag ccagtgacct tcgcgcagta tggatcgcgt cccgcgcaga
     2101 gcgaccggta gaaccgggac accggcctgt gcggcgatga cgaatgcgcc catgcggaaa
     2161 ggaaggagcc cgggcatgcg acggaaagtg ccttccggaa aaaaaccgag cggctcaccg
     2221 ttgtgaatga gctcgagcag gcgttcggtg tccgagagcc cgcgttgcag atcgaagcgc
     2281 tcgacaaact gcgtaccgat tgaacgcag
//
LOCUS       DASURG010000285         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1481150, whole genome shotgun sequence.
ACCESSION   DASURG010000285 DASURG010000000
VERSION     DASURG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481150"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1859
                     /locus_tag="VFY80_04950"
     CDS             42..1859
                     /locus_tag="VFY80_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX6007286.1"
                     /translation="MNFNWRVGLAFAHDLTACAAAWLFSFWLRFNLEHIPPPFDEIAV
                     QSVLIVVPLYGALFLAFGLYRGLWRFASLPDLKRILFAVGLGAIAAPALLFMLQMAVP
                     RSVLIMSPIFLVTVMGGSRLAYRAWKERTLTSLLPQAREPVFVLGAEEAAVNLIKELA
                     RSPQWRVVGVFEDEGSKHGRQLHGVDVLGKLDALPYWREKLSVGHAIIAMPGASHGAR
                     RRVMEFARQAGLKVLTVPSFDDLMSGRVTVSQVRHVELDDLLGRDPVVLDAAGLRSWL
                     ENRVVLVSGAGGSIGAELCRQIARYRPRRLILFELNEFALYTVEQEFRERYGEVDIVP
                     AIGDVKSVARVREIIEAHRPSIVFHAAAYKHVPLMENENAWQAVLNNVAGTQVMAQAA
                     LDHGIEKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQPDGTRFVMVRFGNVLGSTG
                     SVIPKFRDQIARGGPVTVTHPEITRYFMSIPEAVQLVMQAGLMGQGGEIFVLDMGEPV
                     RIADLAHDMIRLSGLTEGEIRIVYTGLRPGEKLYEEPLAADENSLPTPHPKLRIARAR
                     QENGAWVADVSQWLATNDFPSESAVKAQLARFVPEYSGR"
     gene            1870..>2192
                     /locus_tag="VFY80_04955"
     CDS             1870..>2192
                     /locus_tag="VFY80_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007287.1"
                     /translation="MPAAISRYSDHTARWSAIALGFSIPLTVALNNVFLASTLLAWLL
                     GGGYREKLAAFRHPAAIPAIALFALTALGILYSQADLDAAVLHLKKYIDLMLIPVFLY
                     VFRDA"
BASE COUNT          380 a          687 c          697 g          428 t
ORIGIN      
        1 gaacatgcct ggcggcggaa aagcacgagc ggagctgtat gatgaatttc aactggcgcg
       61 tcgggctcgc cttcgcgcac gaccttaccg catgtgccgc cgcatggctc ttctcgttct
      121 ggctgcgctt caatctggag cacataccgc ctcccttcga cgagatcgcc gtgcaatcgg
      181 tgctgatcgt cgttccgcta tacggtgcat tgtttctcgc tttcggactt tatcgcgggc
      241 tctggcgctt cgccagcctc ccggatctga agcgcattct gttcgccgtg ggcctcggcg
      301 ccatcgctgc gcctgcccta ctattcatgc tgcagatggc ggtgccccgc tcggtgctca
      361 tcatgagccc gattttcctc gtcaccgtga tgggcgggag ccggcttgcc taccgggcgt
      421 ggaaggaacg cacgcttacg agcctgctcc cgcaagcgcg cgagccggtg ttcgtcctcg
      481 gcgctgagga agcggccgtc aacctcatca aggagctcgc gcgcagcccg caatggcgcg
      541 tcgtcggggt cttcgaggat gaaggcagca agcacggccg ccagctgcac ggcgtcgacg
      601 tgctcggaaa gctcgacgcc ctgccctatt ggcgcgagaa gctgagtgtg gggcatgcga
      661 tcatcgccat gcccggggca tcgcacggcg cgcgcagacg cgtgatggag ttcgcgcgcc
      721 aggccgggct caaggtgctc actgtcccct ccttcgacga tctgatgagc ggccgcgtca
      781 ccgtatcgca ggtgcggcac gtcgaactgg atgacctgct cggacgcgac ccagtcgttc
      841 tggatgccgc agggctcagg agctggctcg aaaaccgcgt cgttctggta agtggtgccg
      901 gcgggtccat cggcgccgag ctgtgtcgcc agattgcccg ctaccgtccg cgccgtctca
      961 tcctgtttga gctgaacgag tttgcgctct ataccgtcga gcaggaattc cgcgagcgct
     1021 acggggaagt ggacatcgtg cccgcgatag gcgacgtcaa aagcgtggcg cgcgtgagag
     1081 aaatcatcga ggcgcaccgg ccatccatcg tatttcacgc cgctgcgtac aagcacgtgc
     1141 cgctgatgga gaacgaaaac gcctggcagg ccgtactgaa caacgtcgcg ggcacgcagg
     1201 tgatggcgca ggccgcgctc gatcacggca tcgagaaatt cgtgttgatc tcgaccgaca
     1261 aagccgtaaa cccgaccaac gtcatgggcg caagcaagcg gcttgcggag atggtgtgcc
     1321 aggcgctgca acagcccgat ggcacccgtt tcgtcatggt gcgtttcgga aacgtgctgg
     1381 gctcgaccgg cagcgtcatc ccgaagttcc gcgaccagat cgcgcgcggc ggccccgtca
     1441 cggtgacgca tccggaaatc acgcgctatt tcatgtcgat ccccgaggcc gtacagcttg
     1501 tcatgcaggc cggcctcatg gggcaaggcg gagagatctt cgtgctggac atgggggagc
     1561 cggtgcgaat cgcggatctg gcgcatgaca tgatccggct ttcgggcctc accgagggag
     1621 agatccggat cgtctacacc ggcctgcggc cgggcgagaa gctttacgaa gagccgcttg
     1681 ccgccgacga gaactcgctc ccgacaccgc atcccaagtt gcgcatcgcc agggcgcggc
     1741 aggagaacgg cgcgtgggtc gctgacgtgt cgcagtggct cgcgacaaac gattttccct
     1801 ccgaatcggc ggtaaaggcg cagcttgcac gcttcgtccc cgaatattcc gggcgataga
     1861 ccgggcgcga tgccggccgc gatcagtcgc tattccgatc acaccgcgcg ctggtcggca
     1921 atcgcgctcg gcttttccat tccgctgact gtcgccctga acaatgtgtt tctggcctct
     1981 acgctgctgg cgtggctgct gggcggaggc tacagagaaa agcttgccgc gtttcgacat
     2041 ccggctgcga tacctgccat cgcgctgttc gctttgacgg ctttgggcat tctgtacagc
     2101 caggcagatt tggacgctgc ggtgctgcat ctcaagaaat atatcgatct catgctgatt
     2161 ccggtatttc tctacgtatt ccgcgatgca ga
//
LOCUS       DASURG010000286         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1481684, whole genome shotgun sequence.
ACCESSION   DASURG010000286 DASURG010000000
VERSION     DASURG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481684"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VFY80_04960"
     CDS             <1..1605
                     /locus_tag="VFY80_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX6007288.1"
                     /translation="AAVLLRLHEAPMYFYKKGRGRYRAAPEASLKAALAGIERKKQQA
                     LAKQAYVEQLVDSHLPAAFQPMLDRLLYQPDRNSIEWKALEEASVALKLTPAHVIDRA
                     GGIPSTHDYHLKRFLLEYFPRGAGFAQVPDAGEPGELPHAQTQAFSIDDAATTEIDDA
                     FSLARLPSGSWRIGIHIAAPALGVTHGSPLDGIARERLSTVYFPGGKITMLPDEAIRR
                     YTLAEGRACPAVSLYIEITPELEVAGLSNRIEYVHIAANLRHDALETQFNDRTVAEAN
                     IAHPYGDELLTLWRFATKLALARRGEEEATLRPEYSFRVENDRVAITRRQRGTPVDTL
                     VSELMIFANREWGRQLAASATAAIYRVQGNGKVRMSTVPAAHEGLGVEQYIWATSPLR
                     RYVDLLNQRQIVALIRGEPAPLQSRDEALLAAMRDFEVTYEAYGEFQRTMERYWSLRW
                     IIQEKCSALSGRVLRENLVRLDALPLVLRVPSLPDIASGAAVELGVVEIDLLELALRC
                     EFRRRIDVPGVAQTVEAGQPDSATSL"
     gene            1660..2697
                     /locus_tag="VFY80_04965"
     CDS             1660..2697
                     /locus_tag="VFY80_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX6007289.1"
                     /translation="MGRIRLMKALPARKVVPLERRGRHLRHAPWSGAAAQFDERITEL
                     ERRLAAIDHRVVEIASRTRIELALLVSILLHILVISTVSFTMPERKPAADLSPPLDVV
                     LVNAQSPAKPPRADALAQANLDGGGNTDAPRRAKSPFPVREQEKPEQKKEVAARKVEP
                     VQRETRQIMTRPAPKNEVEAEPVPVPVPQPQSEAPVEANATEIVRRGLEIARLEAQIS
                     RDWDSYQKRPRRKFIGARTQEFRFARYIEDWRLKIERIGELNYPQAAREQKAYGSLVV
                     TVSIKANGTLEKVEINRPSGWKILDEAALHIVRLAAPFGAFPPDIAADTDILSITRTW
                     TFTRSDQLIAE"
     gene            2713..>3256
                     /locus_tag="VFY80_04970"
     CDS             2713..>3256
                     /locus_tag="VFY80_04970"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6007290.1"
                     /translation="MTDKYAVIGNPVAHSKSPFIHAEFARATGHDISYVALSAPRDGF
                     RSAALGFREARGRGLNVTLPFKHEAWQLANARQGFAHAAGAVNTLDFRGHDIVGYNTD
                     GIGLVRDIRENLRCGLEGRRILIMGAGGAAYGVIEPLLRERPGEIVVANRTLQKGEAL
                     VAHFAAFASLAARGLAARSYA"
BASE COUNT          602 a         1046 c         1064 g          544 t
ORIGIN      
        1 gctgcagtgc tccttcgcct gcatgaggcg ccgatgtact tctacaagaa gggccgtggg
       61 cgctaccggg ccgcacctga ggcatcgctt aaagccgcgc tcgcgggcat cgagcgaaag
      121 aagcagcagg cgctcgcgaa acaggcatac gtcgagcagc tcgttgattc gcatcttcct
      181 gcggcattcc agccgatgct cgacaggctt ctctaccagc cggaccgtaa ttcgatcgag
      241 tggaaggcac tcgaagaagc gagcgtggcg ctcaagctca cgcctgcgca cgtgatcgac
      301 cgggcagggg gcattccttc gacccacgac tatcatttga agcgatttct cctcgaatat
      361 tttccgcggg gggcaggttt tgcacaggtg ccggacgcag gcgagcccgg tgagctgccg
      421 catgcgcaga cacaggcgtt cagcattgac gacgcggcaa ccacggaaat cgacgatgcg
      481 ttttcgctcg cgcggctgcc gagcggcagc tggcgcattg gcatccacat cgccgcgccc
      541 gcgctcggcg tgacgcacgg ctcgccgctc gatgggattg cccgcgagcg gctatcgacg
      601 gtgtatttcc cgggcggaaa aatcacgatg ctgccggatg aagcgatccg gcgctatacg
      661 ctcgccgagg gacgtgcctg ccccgctgtc agcctgtata tcgaaatcac gcctgagctc
      721 gaggtggcgg ggctctccaa tcgcatcgag tacgtgcaca tcgccgccaa cttgcggcac
      781 gacgcgctgg agacacaatt caacgatcgc accgtggccg aggcaaacat cgcgcatcct
      841 tacggcgacg agctgctgac gttgtggaga ttcgcgacca agctcgcgct cgcccggcgc
      901 ggcgaggaag aggcaaccct ccgtcccgag tacagcttcc gcgtcgagaa cgaccgcgtt
      961 gccatcacgc ggcgccagcg cggcaccccg gtcgatacgc tcgtctccga actcatgatc
     1021 tttgccaatc gggagtgggg ccggcagctt gccgccagcg cgaccgccgc aatctaccgg
     1081 gtgcagggga acggcaaggt gcgcatgagc accgtccctg cggcgcatga agggctcggc
     1141 gtcgaacaat acatctgggc aacttcacca cttcggcgct atgtcgatct cctcaatcag
     1201 cgccagatcg tcgcgctgat ccggggagaa ccggcgcctc tgcagtcgcg ggacgaggcg
     1261 ctgcttgccg cgatgcgcga tttcgaggtg acgtatgagg cgtacggcga attccagcgg
     1321 acgatggagc ggtactggag cctgcgctgg atcatccagg agaaatgcag cgccctctcc
     1381 ggccgcgttc tccgtgaaaa cctcgtgagg ctggatgcgc tgccgctcgt gttgcgcgtg
     1441 ccgtccttgc ccgacatcgc atcgggggca gcggtggagc tgggggtcgt ggagatcgac
     1501 ctcctggagc tcgctctgcg ctgcgaattc cggcgtcgga tcgacgtccc gggcgttgcc
     1561 cagaccgtcg aagccggcca gcccgattcc gcgacttcac tttaacggcg aaggccaagt
     1621 ttttgcagca acaagttttc tgtgcaggcg gcccgcccct tgggtagaat ccgcctcatg
     1681 aaagcgttgc cggccaggaa ggttgtgccg ctcgagcgca gggggcggca tctacggcat
     1741 gctccgtgga gcggcgcggc ggctcagttc gacgagcgga tcaccgagct cgaacgtcgc
     1801 ctggctgcga tcgatcaccg cgtcgtcgaa atcgcctccc gtacgcgcat agagcttgcg
     1861 ctgctggtgt cgatcctgct gcacatcctc gtcatctcca cggtgagctt cacgatgccg
     1921 gagagaaagc cggctgcgga tctcagcccg ccgctcgatg tcgtgctggt gaatgcccag
     1981 tcgccggcca agcctccgag agccgacgcg ctggcgcagg cgaatctcga tggtggcggc
     2041 aataccgatg cgccgcgccg ggccaagagc ccgtttccgg tgcgcgagca ggaaaaaccg
     2101 gaacaaaaaa aggaagtcgc tgcgcgtaag gtcgagccgg tacagcggga gacgcgccag
     2161 atcatgacgc gcccggcacc gaaaaacgaa gtcgaagcgg agccggttcc ggtgccggtg
     2221 ccccagcccc agagcgaagc gccggtcgag gccaatgcaa ccgagatcgt gcggcgcggg
     2281 ctggagatcg cgcgcctcga ggcgcagatc agcagggact gggacagcta ccagaagcgg
     2341 ccgcggcgca agttcatcgg cgcgcgcacg caggagttcc gcttcgcgcg ctacatcgaa
     2401 gactggcggt tgaagatcga gcgcatcggt gaactcaatt acccgcaggc ggcgcgcgag
     2461 cagaaggcct acgggagtct cgtcgtgacg gtgtcgatca aggcgaatgg cacgcttgaa
     2521 aaggtggaga tcaaccggcc atcgggatgg aagatactgg acgaggccgc actccacatc
     2581 gtccggctcg ctgcaccttt cggcgcgttt ccgcccgata tcgcggcgga caccgatatc
     2641 ctcagcatca cgcgcacctg gacctttacc cgctccgacc agttaatcgc cgaataggat
     2701 gcgctgcagt ccatgacgga caagtacgcc gtaatcggca atccggtcgc gcacagcaag
     2761 tcgcccttca tccacgccga attcgcccgc gcgaccgggc acgacatttc gtacgtcgct
     2821 ctttccgcgc cgcgcgatgg atttcgctcc gccgcgctcg gttttcgcga agcacgcgga
     2881 cgcggcctca acgtgacgct gcccttcaag cacgaggcgt ggcagctcgc aaacgcgcgc
     2941 cagggctttg cgcacgcggc gggcgcggtg aacacgctcg atttccgcgg acacgacatc
     3001 gtgggctaca acacggacgg catcggcctc gtccgcgata tcagggaaaa cctgcgctgc
     3061 gggctcgaag ggcgacgaat tctcatcatg ggcgcaggcg gagccgcgta tggcgtcatc
     3121 gagccgttgc ttcgcgagcg gcccggtgag atcgtcgttg ccaatcgtac gctgcagaag
     3181 ggcgaagcgc tggtcgcgca ctttgcggcg ttcgcgtcgc tcgctgcgcg tgggcttgcg
     3241 gctcgaagct acgccg
//
LOCUS       DASURG010000287         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1481780, whole genome shotgun sequence.
ACCESSION   DASURG010000287 DASURG010000000
VERSION     DASURG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481780"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..901)
                     /locus_tag="VFY80_04975"
     CDS             complement(386..901)
                     /locus_tag="VFY80_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX6007291.1"
                     /translation="MLTRRRLYFLLPDVASASDMMNELLLARIEARRIHFLAQRGALP
                     ADLPEATVLQKTDFIHGAQLGMVIGGVAGTMGGLLVVLFPPGGTPLELVTVLVAALLG
                     ALFGAWASSLVASAVPNTQLARFASELEKGKVLMMVDVPLTRINAINELVSRRHPEAI
                     PAGFEPSVVFP"
     gene            1003..1332
                     /gene="rnpB"
                     /locus_tag="VFY80_04980"
     ncRNA           1003..1332
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFY80_04980"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1590..2096
                     /gene="mraZ"
                     /locus_tag="VFY80_04985"
     CDS             1590..2096
                     /gene="mraZ"
                     /locus_tag="VFY80_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6007292.1"
                     /translation="MGEKARSKGVGRLLPAKGGFVFRGIAQLNLDSKGRLAVPARYRD
                     ALVERCSGHLVITADADRCLLLYPLPDWELIQQKLEGLSNLDPRVRDLQRRLIGFAVD
                     VEMDGAGRVLISPALRQYAQLDKNVVLVGQGKKFELWNKDHWEQFIEGAGRLGAGGLP
                     PELEGFSL"
     gene            2099..3046
                     /gene="rsmH"
                     /locus_tag="VFY80_04990"
     CDS             2099..3046
                     /gene="rsmH"
                     /locus_tag="VFY80_04990"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6007293.1"
                     /translation="MGSHRAVLLEGAVQELKVRADGVYVDCTFGRGGHSREILSRLGS
                     QGRLIAIDRDPEAVKAGKSIEDARFTMLHARFSRVGDLVRERGIEFVHGILLDLGVSS
                     PQFDDAARGFSFRGEAPLDMRMDTSGGITAAEWLARATESEIREVIKNYGEERFAKQI
                     AAAIVAARARRPVVSTRELAALVAEAVPAREPRQDPATRTFQAVRIHINQELEELSLA
                     LPQCIELLAPGGRLVVISFHSLEDRIVKRTLRRYATAEAPPPRVPLRAHEIPPPPLRL
                     VGRARRPTPLELAANPRARSAIMRVAERLGSQGLARSAS"
     gene            3046..3327
                     /gene="ftsL"
                     /locus_tag="VFY80_04995"
     CDS             3046..3327
                     /gene="ftsL"
                     /locus_tag="VFY80_04995"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX6007294.1"
                     /translation="MARLTLVLFAILVACALGLVTSQHKARKLYVELQNEQERTKRLE
                     VEWGQLQLEQQTWAMHGRVEKIASDQLDMRVPTPSRIQLVAPPAIGVKP"
     gene            3324..>3594
                     /locus_tag="VFY80_05000"
     CDS             3324..>3594
                     /locus_tag="VFY80_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6007295.1"
                     /translation="MSLAMQARYPLRLPQWRSRLLIVGILACLAVLAARAFYLQGFHY
                     DFLQRKGESRYTRALEIRATRGMIVDRNNEPLAISTPVESVAASPA"
BASE COUNT          656 a         1127 c         1177 g          634 t
ORIGIN      
        1 gatcgcggcg gcgcctgccg cggcatcgcc tttctcctcg caccctcgga cgtagaatcc
       61 acgctggagc tgctctgggc ccgtgaaatg cgacgccgag tctactggcc gcggttcgtc
      121 acggcgcgcc tgcccggcac agccgtgcgt gcactggcat ttatcgcaga ccgccgccac
      181 gcggcttatg ttggccgtct ccccgttggt gcaacggcgc agcgcgtcgc aacgtgctgc
      241 gggcagggcg gagccaacct ggattacctc gcgcgtaccg tggaccatct gctcgaactc
      301 ggcgtgcgcg acgatcgcct ccagcgcgtg cttgcgtctg cgcgcaagct cgcacgcaag
      361 tcgaacggac gagccttagc gcccgctacg gaaaaacgac gctcggctca aagccggcag
      421 gaatggcttc gggatggcgg cggctcacca gctcgttgat cgcgttgatg cgcgtgagcg
      481 gcacatccac catcatcaac actttgccct tctccagttc ggaggcaaaa cgcgcaagct
      541 gagtattcgg taccgcgctc gccacgaggc tcgaagccca ggcgccgaag agcgcaccga
      601 gcagggcggc gaccaacacg gtcacaagtt ccagcggcgt acctccgggc ggaaagagca
      661 ccactagcaa gccacccatc gtgccggcga caccgccgat caccatgcca agctgggcgc
      721 cgtgtatgaa gtcggttttc tggagcaccg tcgcttccgg caggtcggcc ggcaatgcgc
      781 cgcgctgcgc gagaaagtgg atccgccgcg cctcgatgcg cgcgagcagc agctcgttca
      841 tcatgtcgga cgcgctcgcg acgtccggta gcaggaagta gagtctgcgc cgtgtgagca
      901 tgggagtcct ccatttgtcg gtttgcgaca ccctcgctgt cttgggcatc tggcccgcag
      961 gcgagttcgg cgccaccacc gcgactggta aaataacgcc tcgaagctgg ccagacagcc
     1021 gctgcgcatc ggaagcgatg cggagaggaa agtccgggct ccacagggta gggtggcggc
     1081 taacggccgt ccgccgtgag gcgaggaaca gggccacaga gacgagtccg tcggaggcgc
     1141 gcgaaggcgc gtgccggcgg ggtgaaacgc ggcaacctcc acccggagca acaccaagta
     1201 ggcaagcgac gacgcgaccc gccgagcttg cgggtaggtg gcttgagctc tgaagcaatt
     1261 cagagcctag aggaatggct gtcgggagct tgacggctcc tacagaaccc ggcttaccgg
     1321 ccagcttcgc ttgacccgct cccctgttgc aatctcgcaa caggtccctg cccgcgtttc
     1381 ccctagaggc aacgcaacgt tcgtcttcat aaatcacttc taattcaata cttaacgcca
     1441 tgtatcgtaa gtgttatctg tgcacgaaat ctctgttcgt ttggcgtgcg aaggaccccg
     1501 cataagtctt tgtccgagaa aaagaaagta gttggtggcg ccttgaccct tctggtttct
     1561 ttaaatatag tgggtggaag tggtaaaaag tgggagaaaa ggcccgctcc aaaggggtgg
     1621 gtcgacttct gcccgccaaa gggggattcg tgtttcgcgg catcgcgcag ctcaatctgg
     1681 acagcaaggg acggctcgcc gtgcctgcgc gataccgcga cgccctcgtc gagcgctgca
     1741 gcggccatct cgtgatcacg gccgacgcgg accgctgcct tctcctgtat ccgctgcccg
     1801 actgggaact catccagcag aagctcgagg ggctgtcgaa cctggacccc cgcgtgcgcg
     1861 acttgcagcg gcggctcatc ggtttcgcgg tagacgtcga gatggacgga gcgggccggg
     1921 tgctgatctc cccggcgttg cgccagtacg cacagctcga caagaacgtc gtgctagtgg
     1981 gccagggtaa gaaatttgag ctttggaaca aggatcactg ggaacaattc atcgagggtg
     2041 ccggccggct gggcgccggc gggctgccgc ccgaactgga gggcttctcc ttgtgagcgt
     2101 gggctcacat cgggcggtct tactggaagg cgcggtacaa gaactgaagg tgcgtgcgga
     2161 cggcgtttac gtggattgca cgtttgggcg cggggggcac agccgggaga tcctctcccg
     2221 gctgggaagc cagggccggt tgatcgcgat cgatcgcgac ccggaagcgg tgaaggcagg
     2281 aaagtcgatc gaagatgcgc ggttcacgat gttacacgcg cggttcagcc gcgtcggcga
     2341 cctcgtcagg gaacgcggga tcgaatttgt gcacggcatc ttgctcgatc ttggcgtttc
     2401 ctcaccgcag ttcgacgatg cagcacgcgg gttcagcttc aggggcgagg ctccgctcga
     2461 catgcgaatg gatacgagcg gtggcattac cgcggcggag tggctcgcgc gcgcaaccga
     2521 gtccgagatc cgggaggtga tcaagaacta tggcgaagaa cggtttgcta aacagattgc
     2581 agcagcgatt gttgcggctc gagcgcgtag acctgttgtc tcgacacgag aacttgccgc
     2641 gctcgtggca gaagccgttc ccgcgcgcga accccgccag gatccggcga cgcgcacgtt
     2701 tcaggctgta cggattcaca tcaatcagga gcttgaggag ttgtcgctag cgctgccgca
     2761 gtgcattgaa ctgctcgcac ccggcgggcg actggtggtg atcagctttc attcgctcga
     2821 ggatcgcatc gtgaagcgca cgttgcggcg ctatgccacc gcggaggcac ccccgccacg
     2881 cgtgccgctg cgcgcgcacg agattccgcc accgccgttg cgtctcgtgg ggcgcgcgcg
     2941 ccggcccacg cccttggagc tcgctgcgaa cccccgcgcc cgcagcgcca tcatgcgcgt
     3001 ggcggaacgg ctcggctccc aaggcctcgc gcggagcgcg tcatgatggc gcgcttgacc
     3061 ctcgtccttt tcgcaatcct tgtcgcctgc gcgctggggc tcgtcacgtc ccagcacaaa
     3121 gcgcggaagc tctatgtcga gctacagaac gagcaggagc gcaccaagcg cctggaagtc
     3181 gaatggggac aattgcagct cgagcagcag acatgggcga tgcacgggcg cgtggagaaa
     3241 atcgccagcg atcagctgga catgcgcgtg cccaccccgt cccgcataca actggtcgct
     3301 ccacccgcca ttggagtgaa gccgtgagtc tggcgatgca ggcgcgctat cccctgcggc
     3361 tgccgcagtg gcgttcgcgg ctcctgatcg tgggcatcct cgcctgtctc gccgtgctgg
     3421 ccgcgcgcgc gttttacctg cagggcttcc attacgattt cctgcagcgc aagggcgagt
     3481 cgcgctatac gcgtgcgctc gaaattcgcg cgacacgggg catgatcgtg gaccgcaaca
     3541 atgaaccgct cgctatttct acaccggtgg agtcggtggc cgcgagccca gccg
//
LOCUS       DASURG010000288         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_152587, whole genome shotgun sequence.
ACCESSION   DASURG010000288 DASURG010000000
VERSION     DASURG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152587"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFY80_05005"
     CDS             complement(<1..686)
                     /locus_tag="VFY80_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6007296.1"
                     /translation="MKSAFAGPGSYSPSLRVRLLLAGLLVQTVMLALLIVNGIKVMDE
                     KLAERTRAHLDEQKQFLKAVLAPPVAIGDYASAQKVLERVRREDGIAYLVLQDTAGRI
                     VAASGWEHERALPPLKQVAFSEPGVLDTAVPIEMDGRNHGILRFGVSTRFMERARSDL
                     IKDNLAIGVVALILSTLTMVGLSYWLTRSLVRLTRASAQVAAGDLDVRLPVESQDEVG
                     RLTRAFNTMAG"
     gene            complement(706..2118)
                     /gene="argA"
                     /locus_tag="VFY80_05010"
     CDS             complement(706..2118)
                     /gene="argA"
                     /locus_tag="VFY80_05010"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX6007297.1"
                     /translation="MRRVRAPGSTAISDNEDLEAARGKPEHAPARRRLRAQDRFIDWF
                     QSAAPYIHAFRGKSFVIAFGGELVTDGRFVDLAVDLNLLAALGVRLVLVHGARPQTED
                     KLRIQRHRSRFHRGLRVTDDIALGCAMEASGRLRVEIEALLSMGLPNSPMAGADIRVA
                     SGNFITAKPLGVLDGVDFMHTGEVRKVDTVAIQRRLQDGELVLLSPLGYSPTGEVFNL
                     TVEDVAASTAGALKADKLIFLMDTLGVTGRRGELLRELTVKRAQALLARDSIAANDVR
                     FYLPAAVRACQQGVKRAHLISGHLDDALLLELFTHRGVGTLLTPDPLEVLRPARADDI
                     GGILRLIEPLEADGTLVKRNRSLLEREMNRFVVLDHDGMVVACAALYPFPDERAGELA
                     CLAVHPDFRNQGAGERLLREIEVRARRQKLKKLFVLTTRAEQWFIERGFEEAGVDVLP
                     GERRTLYNLQRRSVVLTKRL"
     gene            complement(2190..>2825)
                     /locus_tag="VFY80_05015"
     CDS             complement(2190..>2825)
                     /locus_tag="VFY80_05015"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX6007298.1"
                     /translation="NAGDAVTLFDGRGMEYAAVIVRVARGEVTLDVAQGENVDRESSL
                     AVALAQGISSAERMDYTVQKAVELGVATIQPLQTQRGIVRLDRARALKRCAHWRAIAI
                     AACEQCGRNRIPDVLPVMALREWLGQPAAEHGDVRIVLAPDAGLALKTLPRPRTGATL
                     IAGPEGGLTAEELRDAERAGFVAVRLGPRILRTETAALAALAAMQVLWGDF"
BASE COUNT          492 a          983 c          889 g          461 t
ORIGIN      
        1 cccgccatgg tattgaacgc gcgcgtgagc cggcccactt catcctggct ctccaccgga
       61 agccgcacat cgagatcgcc ggctgccacc tgggcgcttg cccgcgtaag gcgcaccagg
      121 ctgcgcgtga gccagtagga cagtcccacc atcgtcagcg tggaaagaat cagcgcgacc
      181 acgccgatcg cgaggttgtc cttgatcaga tccgagcgcg cgcgctccat gaaccgggtc
      241 gataccccga aacgcagtat gccgtgattg cggccgtcca tctcgatcgg caccgcggtg
      301 tcgagcacgc cgggctcgct gaacgccacc tgcttcagag gaggcagggc gcgttcgtgc
      361 tcccacccgc tcgcggctac gatgcgcccg gcggtgtcct gcagcacgag atatgcgatg
      421 ccgtcttcgc gtcgcacccg ctccagcacc ttctgtgcgc tcgcatagtc gccgattgca
      481 accggcgggg cgagcacggc cttcaggaac tgcttttgct cgtcgagatg ggcgcgggtg
      541 cgctcggcaa gcttctcatc catgaccttg atgccgttga cgatcagcaa cgccagcatc
      601 accgtctgca ccagcaagcc ggcgagcagc agccgcacgc gcaacgacgg tgaatacgag
      661 ccgggaccgg cgaacgcgga tttcatgtgg ggcacgttgc tcgtttcaca ggcgcttggt
      721 caggacgacg gaacggcgct gcaggttgta gagcgtgcga cgctcgcccg gcagcacgtc
      781 cacgccggct tcctcgaaac cgcgttcgat gaaccactgc tcggcccggg tggtgagaac
      841 gaagagcttc ttgagtttct gccggcgcgc ccgtacctcg atctcccgca gcagccgctc
      901 gcccgcgccc tggttgcgga agtcgggatg cactgcgagg caggcaagct ccccggcccg
      961 ttcgtcggga aagggataga gcgccgcgca tgccaccacc atgccgtcgt gatcgagcac
     1021 gacaaagcgg ttcatttcgc gctcgagcag gctgcggttg cgcttgacga gcgtgccgtc
     1081 cgcctccagc ggctcgatca gccgcaggat gccgccgatg tcgtcggcgc gcgcggggcg
     1141 cagcacttcc agcgggtccg gcgtgaggag cgtacccaca ccccgatgcg tgaacagctc
     1201 cagaagcagt gcatcgtcga gatggccgga gatcagatgc gcgcgcttca cgccctgctg
     1261 gcaggcgcgc accgcagcgg gaagatagaa gcgcacgtcg ttcgcggcaa tggagtcgcg
     1321 cgcgagcagt gcctgcgccc gcttcaccgt gagctcgcgc agcagctctc cgcgacgtcc
     1381 ggtgacaccc agcgtgtcca tgagaaagat cagcttgtcg gctttcaacg ctcccgcagt
     1441 cgaagctgcc acgtcctcca ccgtgaggtt gaaaacttca ccggtcgggg aatagccgag
     1501 gggcgaaagc agcacaagct caccgtcctg aagccggcgc tggatcgcga ccgtgtccac
     1561 tttgcgcact tcgcccgtat gcatgaaatc cacgccgtcg agcactccca gcggcttggc
     1621 ggtgatgaag tttccgctcg cgacccggat gtccgcaccc gccatcggcg agttgggcaa
     1681 ccccatggaa agcagcgcct cgatctcgac ccgcaaccgc ccgctcgcct ccatggcaca
     1741 gccgagcgcg atgtcgtctg ttacccgcaa gccgcggtga aaacggctgc ggtggcgctg
     1801 gatcctgagc ttgtcctcgg tctgcggacg cgccccgtgc accaggacca gccgcacccc
     1861 gagagcggca agcagattga ggtccacggc gagatcgacg aaacgcccgt ccgtcaccag
     1921 ctcgccgccg aatgcgatca cgaagctctt gccccggaaa gcgtggatat agggcgctgc
     1981 ggactggaac cagtcgatga accggtcctg cgcgcgcagg cgccggcgcg ctggcgcatg
     2041 ctccggcttc ccgcgcgctg cttcgagatc ctcgttgtcg gaaatggctg tgctccctgg
     2101 cgctctgacg cggcgcaaat cgagattccg ttgctatttc aagtacatgt tatgcgagta
     2161 cgggcgcgaa agaaagcacc aggtgctggc tagaaatcgc cccacaatac ctgcatggcc
     2221 gccagcgcgg ccagcgccgc cgtctcggta cgcagtatgc gcgggccgag ccggaccgcc
     2281 acaaagcccg cgcgctcggc gtcgcgcaac tcttccgctg tcaaaccacc ctccgggccc
     2341 gcgataagcg tagcgccggt gcgcggccgt ggcagcgtct tcaaggcgag gcccgcgtcc
     2401 ggcgcaagca cgatgcgcac atccccgtgc tccgctgccg gttgcccgag ccactcgcgc
     2461 agcgccatca ccggaagcac gtccggtatg cggttacgcc cgcactgctc gcaggcggca
     2521 atggctatcg cccgccagtg cgcacaacgt ttcagcgcgc gtgcgcggtc gagtcgaacg
     2581 atgccgcgct gggtttgcag gggctgtatg gtcgccacac cgagctccac cgctttctgc
     2641 acggtgtaat ccatgcgctc cgcgctcgat atgccctgtg ccagcgcgac agcgagcgat
     2701 gactcgcgat cgacattctc gccctgtgcc acatcgagtg tgacttcgcc tcgcgccaca
     2761 cgcacgatga cggcggcata ctccatgccg cgtccatcga ataacgtcac ggcgtcgccc
     2821 gcatt
//
LOCUS       DASURG010000289         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1276875, whole genome shotgun sequence.
ACCESSION   DASURG010000289 DASURG010000000
VERSION     DASURG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276875"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFY80_05020"
     CDS             complement(<1..258)
                     /locus_tag="VFY80_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007299.1"
                     /translation="MPLDKLSAVLQRHVADLIARGTAKGEESVVVKVMPAQGERGPRF
                     LLAGMGDQQFIRMNSNSYLGLSLQRDIIHEEEQAAESFGAGP"
     gene            complement(245..1195)
                     /locus_tag="VFY80_05025"
     CDS             complement(245..1195)
                     /locus_tag="VFY80_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6007300.1"
                     /translation="MKRILVTGAAGQVGSELVPELRRIYGEEAVLATDIRTPDDASGP
                     YEQLDCTDAQAIAVAVRRHRADTIYHLAALLSATAEAKPQKAYEINMGTLVNVLEVAR
                     ELTAAVFTPSSIGAFGPGTPRDPTPQDTIQRPTTMYGVTKVAGELLCDYYFTRFGVDT
                     RGLRFPGLISYVAPPGGGTTDYAVHIFYQAIEQKSYTCFLSAETQLDMMYMPDAVRAA
                     IEIMEVDPTRLQHRNAFNVTAMQLTPHSLSEMIKRHIPEFTISYDVDPVRQRIADSWP
                     RRLDDSAARAEWGWAPQYDAESMVADMLQKLSEKAAHAAR"
     gene            complement(1425..1547)
                     /locus_tag="VFY80_05030"
     CDS             complement(1425..1547)
                     /locus_tag="VFY80_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007301.1"
                     /translation="MKKGFVVGRCDRKSRQMVILKYIKSPIAGPFSLKAVVAKA"
     gene            complement(1550..2155)
                     /gene="lexA"
                     /locus_tag="VFY80_05035"
     CDS             complement(1550..2155)
                     /gene="lexA"
                     /locus_tag="VFY80_05035"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6007302.1"
                     /translation="MPLTKRQKEILDFLEAFMSENGYAPSFEEIARHFGYTSLATVHE
                     HLENLKQKGYIRKSYNASRSLELVPSGTHASAVELPLLGNVAAGIPIEAVTDNETIAV
                     PEDFLTRGGEHYVLRVKGDSMIDEQIRDGDFVIVNARRTASNGEMVVALVGGESATVK
                     KFYRENGRVRLQPANATMPPMFYREQEVAVQGIVIGVIRRY"
     gene            2751..>3537
                     /locus_tag="VFY80_05040"
     CDS             2751..>3537
                     /locus_tag="VFY80_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007303.1"
                     /translation="MNFSDNGDTELEPASASAAEPSPADTGSGQRTPVVQAQSPPAGE
                     LTPREVGEFFTALDKAVRARRLYAANNPAYKAFVANLRTNVTALWDGAHALTVVVDES
                     GFRWHDETFSPGEGREHLAFQFYKDGIRALTFLPGFEQEIERFLDVVARARQIDSTSA
                     DDMVTLLWEQEFASLLYNYVDALVEGLEIPETGPIPIPFDKLDLTLVSADVTGSDESA
                     TTLPPAVQAGMPTVAQTISRDDFEETLYFLEHGELEYLRQEVER"
BASE COUNT          678 a         1108 c         1045 g          706 t
ORIGIN      
        1 cggacccgcg ccaaaacttt ccgcagcttg ttcttcttcg tgaatgatgt cgcgctgcag
       61 tgacaggccg aggtatgaat tcgagttcat gcggatgaac tgctgatcgc ccatgcctgc
      121 gagcaggaaa cgcggtccgc gctcgccctg cgccggcatc actttgacga cgacggattc
      181 ttcacctttt gcggtgccgc gtgctatcag atctgcaacg tgccgctgca agacggcgga
      241 aagtttatcg agcggcatgc gcggctttct ccgaaagttt ctgcagcatg tcggctacca
      301 tggattcagc atcgtattgt ggtgcccagc cccattccgc acgcgccgca ctgtcatcca
      361 ggcgacgcgg ccaggaatca gcaatgcgct ggcgcaccgg atcgacgtcg tagctgattg
      421 tgaattcggg aatgtggcgt ttgatcattt cggacagcga gtgcggcgtg agctgcatgg
      481 cggtgacgtt gaatgcattg cggtgctgca ggcgcgtcgg atcaacttcc atgatttcaa
      541 tggccgcgcg taccgcatcc ggcatgtaca tcatgtcgag ctgcgtttcg gcagatagga
      601 aacacgtgta gctcttctgc tcgatcgcct ggtagaaaat gtgaaccgcg tagtcggtag
      661 tgccgccgcc cggtggtgcg acataagaga tgagcccggg aaaacgcagg ccgcgcgtgt
      721 ccacgccgaa acgcgtgaag taatagtcac agagcagctc accggcgacc ttggtcacgc
      781 cgtacatcgt ggtcggccgc tggatggtat cctgcggcgt tggatcgcgc ggtgtaccgg
      841 gcccgaacgc gccgatactg ctgggcgtga agaccgcggc cgtaagctcg cgtgcgacct
      901 ccagcacatt gaccagcgtg cccatgttga tttcgtaggc tttttgcggc ttggcttccg
      961 ccgtcgccga caacaatgcg gccagatgat agatggtgtc ggcgcgatgg cgccgtaccg
     1021 ccactgcaat tgcctgcgcg tccgtgcagt ccagctgctc gtacggtccg ctcgcatcgt
     1081 cgggcgtccg tatgtccgtc gccaggaccg cttcctcgcc atatatcctg cgcagctcgg
     1141 gcaccagctc ggacccaacc tgtccggccg cacccgtaac cagaatccgc ttcattctat
     1201 cgccactccg tgattgcacc gcccccggcc gcggcccggg cgcacgaaag gatagccgct
     1261 cggtgaacgg tcgcaaagtg ttgaaccgcc ggacgctgca cgtctcgaca gactcgggtg
     1321 gcgtgatgtt cggaggatct tgttttacgg caggtggagg agtaacgagc tggcgaggcg
     1381 cgcaggttcg cccgggtgca ccctgcgcgc tctcgccatt cgcttcaggc cttggcgacg
     1441 accgccttca acgaaaacgg gcccgcaatc ggcgatttga tgtacttcag aatgaccatc
     1501 tggcgcgact tgcgatcgca acgacccacc acaaaaccct tcttcatcgc tagtacctcc
     1561 ggatcacgcc gatgactatg ccctggacgg caacttcctg ctcgcgataa aacatgggag
     1621 gcatggtggc attcgcaggc tgcagccgca cccggccatt ctcgcgatag aatttcttga
     1681 ccgtggccga ctcgccaccc accagcgcca cgaccatctc gccattcgac gccgtccgtc
     1741 gcgcgtttac gatgacgaaa tcaccgtcgc ggatctgctc gtcgatcatg gagtcgccct
     1801 ttacacgcaa cacgtagtgc tcgccgccgc gcgtgaggaa atcttccggt acggcaatgg
     1861 tctcattgtc cgtgacggcc tcgatcggaa tgcccgccgc cacgttcccc agcagcggca
     1921 gctccaccgc cgacgcatgc gtcccgctcg gcaccagctc cagcgaccgg ctcgcattat
     1981 agcttttccg gatgtagccc ttttgcttga gattctccaa atgctcatgc accgtcgcca
     2041 gcgacgtgta gccgaaatgc cgggcgatct cttcgaaact gggcgcgtac ccattctcgc
     2101 tcataaatgc ttcgagaaag tccaggattt ccttctgtcg cttcgtcagg ggcatggctc
     2161 aagcctcgcg agggatttcc gtaccgaaaa tatgccgaaa accagtatat ccgaagatag
     2221 accgaaatgc aagcatcaca ggttttgggg caaaccgcga ccgccgggag gacattcact
     2281 caagctcgag ttgaagcgtc gctcccccgg gacgtgcgaa ccgaggggtg ggtcacggaa
     2341 ttgtcaaagc atatagtgac cacggcgcca acgccataac cgctgcgggc gtgaattttc
     2401 ccggcccgcg ctcttgacag acgggtgcgg cattgccttt gacgtgcgcc ccgatgtcgt
     2461 caatgaccaa atcgacgctc actcgtcatc atcgtaagcg cgcgcgtgcc ggcgtccgca
     2521 actcatgcaa ccatatgcaa ttgggtggcg aacgcccagg gccgccgcta tattgtcgga
     2581 tccgatattg ggtccgattc atacctccgc atggtcccgc gatttcctcg tgctgcgatg
     2641 aaatcaaaag accggttcca cacatacaga aggcggctgc cgaaggcggt agtgggcatg
     2701 cgtcggcggt tcgaacgcaa gaggcgttta cgtatccctg aatcatcagc atgaacttct
     2761 ccgacaacgg cgataccgaa ttagagccag cgtctgcgag cgcggccgag cccagccctg
     2821 ctgatacggg gagcgggcag cgcacgcccg tcgtgcaggc gcagtcgccg ccggccgggg
     2881 agcttacgcc gcgcgaggtg ggtgagttct tcaccgcact cgacaaggcc gtccgtgccc
     2941 ggcggctgta tgccgcgaac aaccccgcct ataaagcttt tgtcgccaat ctgcgcacca
     3001 acgtgaccgc tctctgggat ggtgcccatg ccctcaccgt cgtggtggat gagagcggct
     3061 ttcggtggca tgacgaaacc ttttcaccgg gcgaaggtcg cgagcacctg gcttttcagt
     3121 tctacaagga cggtatccgc gcgctcacct ttctgcccgg cttcgagcag gagatcgagc
     3181 gtttcctcga tgtagtggcg cgcgcgcggc agatcgacag caccagcgcc gacgacatgg
     3241 tcaccttgct ttgggagcag gaattcgcct cgctactcta caactacgtc gacgcgctgg
     3301 tcgaagggct ggaaattccg gaaaccggcc cgatcccgat tccattcgac aagctcgatc
     3361 tcacgcttgt ttctgccgat gtgacgggca gtgacgagag cgcgaccacc ctgccgccgg
     3421 ccgtgcaggc gggcatgcca acggtggcgc aaaccatttc gcgcgatgat ttcgaggaga
     3481 cgctgtactt cctcgagcat ggcgagctgg aatacctgcg tcaggaagtc gaacgcg
//
LOCUS       DASURG010000290         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_153453, whole genome shotgun sequence.
ACCESSION   DASURG010000290 DASURG010000000
VERSION     DASURG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153453"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFY80_05045"
     CDS             complement(<1..561)
                     /locus_tag="VFY80_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6007304.1"
                     /translation="MTESHAPMTPTALPLRDRRGRPLRDLRVSVTDRCNFRCVYCMPK
                     EVFGKDYAFLERKALLTFEEIARLVRLFTGEGVTKVRLTGGEPLVRRNLERLVAMLAA
                     MPGLDLTLTTNGALLLQKARALKEAGLKRITVSLDSLDDAVFRSMNDVDFPVSRVLAG
                     IDAAAEAGLAPIKINMVVKRGLNEESV"
     gene            715..1206
                     /locus_tag="VFY80_05050"
     CDS             715..1206
                     /locus_tag="VFY80_05050"
                     /inference="COORDINATES: protein motif:HMM:NF023177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEX6007305.1"
                     /translation="MSSPTGFFPRRSVAVIMERARLTNRWVQEQWSARAVLCDEVPHE
                     APHRVIVEHADSLQILYPGRHVALRPDEAEGYYFNVTAPQPRVFVLWRMHDGIARPEM
                     LTVSYHEGARWMDSDEHVDGVPLPPELMPWIGEYVERHYRPEPPKAKRYASSKDRGRM
                     GRF"
     gene            1215..1742
                     /locus_tag="VFY80_05055"
     CDS             1215..1742
                     /locus_tag="VFY80_05055"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEX6007306.1"
                     /translation="MAEEKEPFLARWSRVKREAQVEADTQAGTPREAAQEPPPELPPV
                     EKLGFESDYTGFFHPKVTDETRRAALKRLFSDPHFNVMDGLDVYIDDYSKPSPLPAEM
                     LAGLKQAQRILAWAAENLEQGSKDEPAAVGDTSRAGLDVPNTDVVVPANPSGASAGAE
                     PVPANPSPEQGAESK"
     gene            1780..>2352
                     /locus_tag="VFY80_05060"
     CDS             1780..>2352
                     /locus_tag="VFY80_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HEX6007307.1"
                     /translation="MTQDKRKTLLCSCNRTMALDGQRLAQALGWPTAPRIDDELCRRH
                     VASFEAAIKTGDDVVVACTQEAPLFSELHSELSARGRITFVNIRETAGWSTQARQATP
                     KIAALLALASLPDPEPVPAVTYKSNGSVVIIGPAETALYWAERLADRLEVCVLFTDSG
                     ARAELPSERCYPVYSGSVKSVIGYLGNFELS"
BASE COUNT          438 a          742 c          779 g          393 t
ORIGIN      
        1 aacgctctct tcgttcaagc cgcgcttgac gaccatattg atcttgatcg gcgcaagccc
       61 cgcttccgcg gcggcatcga tcccggcgag cactcgcgag accggaaaat caacgtcgtt
      121 catgctccgg aacacggcat catcgaggga gtcgaggctt accgtaatgc gtttgagtcc
      181 ggcttccttg agcgcacggg ccttctgcaa aaggagtgca ccgttggtcg tgagcgtgag
      241 atcgagcccc ggcatggcag cgagcatcgc gaccagccgc tcgagattac gccgcacgag
      301 cggctcgccc ccggtcaatc tcaccttggt cacgccctcg cccgtaaaca agcgcacgag
      361 ccgggcgatc tcctcgaacg tgagcagcgc cttgcgttcc aggaaggcgt agtccttgcc
      421 gaagacctcc tttggcatgc agtacacaca gcgaaagttg cagcggtccg tgaccgagac
      481 acgaaggtcg cgcagcggcc ggccgcggcg atcccggagt gggagcgccg tcggggtcat
      541 cggggcgtgg ctctcggtca tgaattgagt gctggggcgg tcaggcgaca tccatggacg
      601 gggtccatta tggcaaggcc cccatggcgg cgcaaggaaa ccattacttg taaatcaggt
      661 atttgaagcg ctttcttgtt gccatcgccg gcgcggctgg cgcacaatca cgccatgtcg
      721 agtccgaccg gattcttccc gcgccgttcc gtcgccgtaa tcatggagcg cgcgcgactc
      781 acgaaccgtt gggtgcagga gcagtggagc gcgcgcgcgg tgttgtgcga cgaggtgccg
      841 cacgaggcgc cgcaccgcgt gattgtcgag cacgcggatt cgctgcagat cctctacccc
      901 gggcggcacg tggctctgcg cccggatgag gctgaaggct attatttcaa cgtgactgcg
      961 ccgcagccgc gcgtattcgt gctctggcgc atgcacgatg gcatcgcgcg gcccgaaatg
     1021 ctgacagtga gttaccacga gggcgcgcgc tggatggaca gcgacgagca cgtggacggc
     1081 gtgcccttgc caccggagtt gatgccgtgg atcggcgagt acgtcgaacg ccactatcgc
     1141 ccggagccgc caaaggcgaa gcgctatgcg agcagcaagg accgcggccg catgggacgt
     1201 ttctagtacg caccatggcg gaggagaaag agccgttcct cgcccgctgg tcgcgggtga
     1261 agcgtgaggc gcaggtagag gcggacacgc aggccggtac gccacgcgag gcggcgcagg
     1321 agccgccgcc cgagctgccg cccgtggaaa agctcggttt cgagtcagac tacaccgggt
     1381 ttttccatcc caaggtcacg gatgagacgc gccgcgcggc gctcaagcgg ctcttcagcg
     1441 acccgcactt caacgtgatg gacggactgg acgtgtacat cgacgactac tccaagccga
     1501 gcccgctgcc cgccgaaatg ctggcgggtc tcaagcaggc gcagcgtatc ctcgcctggg
     1561 cggctgagaa cctggaacaa ggctcgaagg acgaaccggc cgcggttggc gatacgtccc
     1621 gcgccgggct cgatgtcccg aacaccgatg tagtggtgcc ggcgaacccg agcggtgcca
     1681 gtgccggtgc cgagcccgtg cccgcgaacc cgtcgccaga gcagggcgca gagtcgaaat
     1741 aaaccggaag ttcatggctg gcgctgagga gcgcctctga tgacccagga caagcgaaaa
     1801 acactgttgt gcagttgcaa tcgcaccatg gccctcgatg gccagcgcct ggcgcaagcg
     1861 ctgggatggc cgaccgcgcc ccgcatcgac gatgaactgt gccggcggca tgtggcgtca
     1921 ttcgaggcgg cgatcaaaac cggggacgat gtcgtcgtcg cctgcacgca ggaagcgccg
     1981 ctgttcagcg aattgcacag cgaactcagt gcacggggcc gcatcacgtt cgtcaatatc
     2041 cgggagaccg ctggatggtc gacgcaagcg cgacaggcaa cccccaaaat cgcggcgttg
     2101 ctggcgctcg ccagccttcc ggatcccgaa cccgttcccg ccgtcaccta caagtcgaac
     2161 ggaagcgttg tcatcatcgg gccggcggag accgcgctct attgggcgga gcggctggct
     2221 gatcgcctcg aggtgtgcgt actctttacc gattccggag cgcgtgccga gcttccgtcc
     2281 gagcgctgtt acccggtgta ttcggggagc gtcaagtcgg tcatcggcta tctgggcaat
     2341 ttcgagctct cc
//
LOCUS       DASURG010000291         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_358388, whole genome shotgun sequence.
ACCESSION   DASURG010000291 DASURG010000000
VERSION     DASURG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358388"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /gene="nuoL"
                     /locus_tag="VFY80_05065"
     CDS             complement(<1..1876)
                     /gene="nuoL"
                     /locus_tag="VFY80_05065"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6007308.1"
                     /translation="MQSQALEHAFEPTLLWAIAILPLLGFLINGAIALKAPEKKGIST
                     IVGPGVIGLAFVIALINFFRMLGADLHDPVIEHYWQWMPVGNLQVAAAFQLDQLSMLM
                     TLIITGVGFLIHVYSIGYMHADPGYARYFAYINLFVFFMLVLVLGANFPLMFVGWEGV
                     GLCSYLLIGFWFRDREKADAGKKAFIVNRIGDFGFLVAMFMIFSNLGTLDFTGVFAAA
                     PTAFPYGSAVVTAITLFLFLGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAG
                     VYLVARSSVLFALAPTTMIVVAGIGALTALFAATIGLIQYDIKKVLAYSTVSQLGYMF
                     IGVGVGAYTAGVFHLMTHAFFKALLFLGSGAVIHAMHEALHHTHKDWDAQDMRNMGGL
                     KKYMPVTWVTMWIGTLAIAGVWPFAGFFSKDEIIWQTAARAVGPFAGWFTLFWVMALI
                     AAMLTAFYMTRLMIMTFHGQSRLSDDEQHHLHEVSPVMWVPLAVLAVASIVGGWINVP
                     HAIEETILGIVPSSEWLHEWLHPVTQGAEAVFAQQGIALSESAPFGGGEALWAALSFA
                     LATAVIVIAAALLVKRPVRNALEAPAERGFARVLYRKWYVDELYDMLIVKPLVGASKF
                     A"
     gene            complement(1877..2170)
                     /gene="nuoK"
                     /locus_tag="VFY80_05070"
     CDS             complement(1877..2170)
                     /gene="nuoK"
                     /locus_tag="VFY80_05070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6007309.1"
                     /translation="MEPRTLILSVILFSLGVFGVLIRRNAIIMFMCVELMLNAVNLAF
                     VVFSRQAGIDGQVFVFFVMAVAAAEAAVGLALVIAIFRHLDTVDIKNFNLLRW"
BASE COUNT          497 a          730 c          730 g          347 t
ORIGIN      
        1 aggcgaactt cgacgcgccg accagcggct tcacgatgag catgtcgtac agctcgtcga
       61 cgtaccactt gcgatagagc acgcgcgcga agccgcgctc cgcgggcgcc tcgagcgcgt
      121 tgcgcaccgg ccgcttcacg agcagcgcgg cggcgatgac gatgacggcg gtcgcgagcg
      181 cgaaggagag cgcggcccag agcgcctcgc cgccgccgaa gggcgcgctc tcgctgagcg
      241 cgatgccctg ctgcgcgaac acggcctcgg cgccctgcgt gaccgggtgc agccactcgt
      301 gcagccactc ggagctgggc acgatgccga ggatggtctc ctcgatcgcg tgcggcacgt
      361 tgatccagcc gccgacgatg gaggcgaccg cgagcacggc gagcggcacc cacatcaccg
      421 gcgagacctc gtgcaggtgg tgctgctcgt cgtcggagag ccgcgactgg ccgtggaagg
      481 tcatgatcat gagccgcgtc atgtagaacg cggtcagcat ggccgcaatc agtgccatca
      541 cccagaagag cgtgaaccag ccggcgaacg gaccgaccgc gcgcgccgcc gtctgccaga
      601 tgatctcgtc cttcgagaag aagccggcga agggccagac gcccgcgatg gcgagcgtgc
      661 cgatccacat cgtgacccag gtgacgggca tgtacttctt cagaccaccc atgttgcgca
      721 tgtcctgcgc atcccagtcc ttgtgcgtgt ggtgcagcgc ttcatgcatg gcgtgaatca
      781 ccgcgcccga tcccaggaac agcagggcct tgaagaaagc atgcgtcatc aggtgaaaca
      841 cgcccgcggt gtaggcgccc acgcccacac caatgaacat gtagcccagc tgcgaaacgg
      901 tcgaatacgc gaggactttc ttgatgtcgt attggatgag cccgatggtc gcggcgaaca
      961 acgcggtcag ggcgccgata ccggccacca cgatcatggt ggtaggcgcg agtgcgaaca
     1021 gcacgctcga acgcgcgacc aggtagacgc cggccgtaac catggtggcg gcatggatca
     1081 gtgcgctgac cggtgtcgga ccggccattg cgtccggcag ccagacgtag agcgggatct
     1141 gcgcgctctt gccggtcgcg cccaggaaga ggaaaagcgt gatcgcggtt accacggcgc
     1201 tgccgtacgg gaaggccgtg ggcgcagccg cgaacacgcc cgtgaaatcg agcgtgccca
     1261 ggttgctgaa gatcatgaac atggcaacca ggaagccgaa gtcgccgata cggttcacga
     1321 tgaacgcctt cttgcccgcg tccgctttct cgcgatcccg gaaccagaag ccgatcagca
     1381 ggtagctaca caagccgacg ccttcccagc ccacgaacat gagcgggaaa ttcgcgccca
     1441 gcaccagcac cagcatgaag aacacgaaca ggttgatata cgcgaagtag cgcgcatatc
     1501 ccggatccgc gtgcatgtag ccgatgctgt aaacgtgaat caggaagccg acgccggtga
     1561 tgatcagcgt catcagcatg gacagctgat cgagctggaa ggcggccgcc acctgcaggt
     1621 tgccgaccgg catccactgc cagtagtgct cgatcacagg atcgtgcaag tcggcgccca
     1681 gcatcctgaa gaaattgatg agcgcgatca cgaaggccag accgatgacg cccggcccga
     1741 cgatcgtgct aatgcccttc ttttccggag ccttcagtgc gattgcgccg ttaatcagaa
     1801 atccgagcag cggcagtatg gcaatcgccc acagcagcgt cggctcgaat gcatgctcca
     1861 aagcctggct ttgcatttac cagcgcaaga ggttgaagtt cttgatgtcg acggtgtcga
     1921 gatggcggaa gatcgcaatg accagcgcca gccccaccgc ggcttccgca gctgcgaccg
     1981 ccatgacgaa gaagacgaat acctgtccgt cgatgcccgc ctgacgtgag aacaccacga
     2041 acgccaggtt gacggcgttc agcatcaact caacgcacat gaacatgatg atcgcgttgc
     2101 ggcggatgag cacgccgaag acgcccagcg agaacagaat gacggacaga atcagggtgc
     2161 gcggttccat tacactctcc gcttggctac cagcacggcg ccgataatgg ccaccagcag
     2221 caggatcgag gtcagctcga agggcacgag gaaatcgcgg aacagcggct gtgcaatggc
     2281 gcctaccgcg cccagctcgc gcac
//
LOCUS       DASURG010000292         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_664877, whole genome shotgun sequence.
ACCESSION   DASURG010000292 DASURG010000000
VERSION     DASURG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664877"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFY80_05075"
     CDS             <1..1095
                     /locus_tag="VFY80_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX6007310.1"
                     /translation="QREIRTRILGVASHVQVSGPNNMLENWREVAAQAARHEQVEAVA
                     PYVSAQGLVTHGSAVRGAFVRGILPEQEDKVAEFDKHMRSGRLDALRAGEYGVILGGQ
                     LARALGVTTGDRVVVVAPQGQVTPAGIMPRLRQFTVVGTFNVDHFEFDSGLALVHLDD
                     ARNLFRFGDAVSGVRLKLKDLFQARTVARELTQMIPSDVYISDWSMQHANLLRAIQIE
                     KRMMFLIIFLIIAVAAFNIVSSLVMAVKDKQADIAILRTLGASPGGITKIFLVQGMMV
                     GVLGTVLGVTSGVLLALNVDSAVLFIETLFGFKFFAKDVYLISDLPSELQMPDVVITA
                     VASFVLSLLATLYPSYRASKVNPAEALRYE"
     gene            1088..1798
                     /gene="lolD"
                     /locus_tag="VFY80_05080"
     CDS             1088..1798
                     /gene="lolD"
                     /locus_tag="VFY80_05080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEX6007311.1"
                     /translation="MSENAPPLTDTNGPVLTCRGVRKTYRQGRIEVPVLLGVDLDVAR
                     AERVAIVGASGSGKSTLLHLLGGLDEPTAGDIRVLGRDLVRQSEAERGHTRNHALGFV
                     YQFHHLLPEFTALENVAMPLFIRRTPVEEAKRAATEVLEKVGLAHRLHHTPAELSGGE
                     RQRVALARALVTRPACVLADEPTGNLDRHNAEAVFGLMLELNRTMGTSFVIVTHDEAI
                     AAKADRVLRLLDGVLQTA"
     gene            complement(1810..>2017)
                     /locus_tag="VFY80_05085"
     CDS             complement(1810..>2017)
                     /locus_tag="VFY80_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007312.1"
                     /translation="ALPVLGAGVALALIGWVWTGLGIIAFGIVAPRLIKRSAPHFVLT
                     RAIEDESVYCEVTQSNILRLSELP"
BASE COUNT          365 a          627 c          659 g          366 t
ORIGIN      
        1 cagcgcgaaa ttcgcacgcg tatcctcggc gtcgcgtcgc atgtgcaggt gagcgggccc
       61 aacaacatgt tggagaactg gcgcgaggtg gcagcccagg cggcgcgcca cgagcaagtg
      121 gaggccgtgg cgccttacgt cagcgcgcag gggctggtga cccatggctc ggcggtgcgc
      181 ggcgctttcg tgcgcggaat tttgccggag caggaagaca aggtcgccga gtttgacaag
      241 cacatgcgaa gcggccgcct cgatgcgctg cgtgccggcg agtacggcgt catcctcggc
      301 ggtcagctcg cgcgtgcatt gggggtgacg acgggagacc gggtcgtcgt cgttgcgccg
      361 caaggacagg tcacgccagc gggcatcatg ccgcggctca ggcaattcac tgtagtgggg
      421 accttcaacg tcgatcattt cgaattcgac tccggactcg cattggtgca cctggacgat
      481 gcgcgcaatc tcttccgctt cggagatgcc gtttccggcg tgcggctgaa gctgaaggac
      541 ctcttccaag cgcgcacggt agcgcgcgaa ctgacgcaga tgatcccgag cgatgtctat
      601 atcagcgact ggtccatgca gcacgcgaac ctgctgcgcg cgatccagat cgagaaacgc
      661 atgatgttcc tcatcatctt tctcatcatt gcggtcgccg cgttcaacat cgtgtcgagc
      721 ctcgtcatgg cggtgaagga caagcaggca gatatcgcga tcctccgcac gctcggtgcg
      781 agtcccggcg gcatcaccaa gatcttcctc gtgcaaggca tgatggtggg tgtcctcggc
      841 acggtgctcg gagtgacgag tggcgtgctg cttgcgctca acgtcgatag cgccgtgctg
      901 ttcatcgaga cgcttttcgg attcaagttc ttcgcgaagg atgtgtacct catatccgat
      961 ctgccgtctg agcttcagat gccggatgtc gtcatcaccg cagtcgcttc ttttgtgctg
     1021 agtctcttgg cgacgcttta tccgagctat cgcgcatcga aggtgaaccc ggccgaagcg
     1081 ctgcgctatg agtgagaacg cgccgccatt gaccgacacg aacggcccgg ttctcacctg
     1141 ccgcggcgtg cgcaagacct atcggcaggg cagaatcgaa gtcccggtgc tgctcggtgt
     1201 ggacctcgac gtcgcgcgcg ccgagcgggt ggcgatcgtg ggcgcatccg gttctggaaa
     1261 gagcacgctc ctgcatctgc tgggtgggct cgacgagccc acggcagggg atatccgtgt
     1321 cctcggccgc gatctcgtcc gccagagcga ggcggagcgc ggccataccc gcaatcacgc
     1381 cctgggtttc gtgtatcagt ttcaccatct gctgcccgag ttcaccgcac tcgaaaacgt
     1441 cgccatgccg ctcttcatcc ggcgcacgcc ggtagaggaa gcgaaacgcg cggccacaga
     1501 ggttctggaa aaagtcggac ttgcacaccg gcttcaccac actccggcgg aactttcagg
     1561 cggagagcgc cagcgcgtag cgcttgcccg agcgctcgtc acgcggccgg catgcgtact
     1621 cgcggatgag ccgacgggca atctcgaccg tcacaatgcc gaagccgtat tcggcctcat
     1681 gctcgagctg aaccgcacga tgggcacgag cttcgtcatc gtcacgcacg atgaagcgat
     1741 cgcggccaag gcggaccgcg tcctgcggct gctcgacggc gtgctgcaga ccgcctgacg
     1801 gcggcccggt caaggaagct cggaaaggcg caggatgttc gactgcgtga cttcgcaata
     1861 gacactttca tcctcaatcg cccgggtgag gacgaagtgc ggtgcggagc gcttgatgag
     1921 ccgcggggcc acgatgccga acgcgatgat gccaaggcct gtccagaccc acccgatgag
     1981 cgcgagtgcg acgccggccc cgagcacggg cagcgcg
//
LOCUS       DASURG010000293         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_970923, whole genome shotgun sequence.
ACCESSION   DASURG010000293 DASURG010000000
VERSION     DASURG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970923"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFY80_05090"
     CDS             <1..342
                     /locus_tag="VFY80_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX6007313.1"
                     /translation="DMQAQFMALNSVASGTAIVTETIFENRFMHVQELKRLGADIEVE
                     GNTAVVKGVPRLDGAAVMATDLRASAGLVLAGLVARGTTVVDRVYHLDRGYECIEEKL
                     SRLGARIRRAR"
     gene            385..1020
                     /gene="hisG"
                     /locus_tag="VFY80_05095"
     CDS             385..1020
                     /gene="hisG"
                     /locus_tag="VFY80_05095"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX6007314.1"
                     /translation="MITIALSRGRILDETLPLLEAAGIRIAEDLLNSRKLVLETNRRD
                     VRVIIVRAADVPTYVQYGAAELGVAGKDVLDEHGGQGLYQPLDLGIARCRMMVAVPRE
                     FDYARVARQGARLKVATKYVQTAREHFATKGVHVDLIKLYGSMELAPLTGLADAIVDL
                     VSTGNTLRANNLKAVEEIAPVSARLVVNQAALKLRRAQIQPLLDAFARAVS"
     gene            1014..2321
                     /gene="hisD"
                     /locus_tag="VFY80_05100"
     CDS             1014..2321
                     /gene="hisD"
                     /locus_tag="VFY80_05100"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6007315.1"
                     /translation="MMTSVRRLDTGHPDFEAQLQALLAFENAHDPAVESAVAGILEDV
                     KARGDEALLEYTRRYDRMSVPDAARLEIPRPELQRALNALSPAQREALAAAERRVRSY
                     HEKQLARSWSYVEPDGTELGQKITALDRVGLYVPGGKAAYPSSVIMNAVPARVAGVRE
                     IIMTVPTPDGERNDLVLAAALLCQVDRVFTVGGAQAIAALAYGTATIPAVDKIVGPGN
                     AYVAAAKRRVFGVVGIDMLAGPSEIVVVCDGGTDPDWIAMDLFSQAEHDELAQAILLT
                     PDPRFIDAVAASIERQLAAMPRREVIRAALRSRGALIRVRDLADACALVNRIAPEHLE
                     LSVADPDALLPKIRHAGAIFLGRYTSEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQ
                     KRTSVIRVSREGARQLGEIAAELARGEGLSAHALAAEYRMKGP"
     gene            2325..>3229
                     /locus_tag="VFY80_05105"
     CDS             2325..>3229
                     /locus_tag="VFY80_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6007316.1"
                     /translation="MAKPETLIRDAVLAMNAYHVPYSAGMVKLDAMENPYTLPAALRE
                     EIAKLAQQAAYNRYPDARATQLRVRLREVMGVPDAADILVGNGSDELIQTLMLATARP
                     GALVLGVEPSFVMFRLIAEFCGMRFLGVPLDAGFELDAERTVDAIRAHRPALVILAYP
                     NNPTGNLYDDEAMARVIAAAPGLVLVDEAYHAFAGRSCMRQLAGNSSLLVMRTLSKLG
                     LAGLRLGMLAGAPEWLEHLDKVRLPYNVNVLTQLIAERVLQHHEVLAKQAASIRAERT
                     RVFEALAALPGVQPYRSDANFILFRV"
BASE COUNT          549 a         1039 c         1075 g          566 t
ORIGIN      
        1 gacatgcagg cccagttcat ggcgctcaac agcgtcgcga gcggcacggc gatcgtcacc
       61 gagaccatct tcgaaaatcg cttcatgcac gtgcaggagc tcaagcggct cggtgccgac
      121 atcgaggtgg agggcaatac cgcggtggtg aaaggggtgc cgcgcctcga cggcgcggcg
      181 gtcatggcaa ccgatctgcg cgcctccgcg gggctggtcc tcgcgggtct cgttgcgcgc
      241 ggcacgaccg tggtcgatcg cgtatatcac ctcgatcgcg gctatgaatg catcgaggag
      301 aagctgtcgc ggctcggcgc gcgtatccgc cgcgcccgct gagtatcgaa tgctaaaatg
      361 ctgttttagt taggctcatg tcccgtgatt accatcgcgc tgtcgcgcgg ccgcattctc
      421 gacgagactc tgccgctgct cgaagcggcc ggcatccgta tcgcggaaga cctgctgaat
      481 tcacgcaaac tggtgcttga gacaaaccgg cgcgacgtgc gggtcatcat cgttcgtgct
      541 gcggatgtgc caacctacgt gcagtatggc gctgcggagc tcggtgtggc gggcaaggac
      601 gtcctcgacg agcacggcgg ccaggggctt tatcagccgc tcgatctcgg gatagctcgc
      661 tgccgcatga tggtcgccgt gccgcgcgag ttcgactatg cgcgcgtggc gcgacagggt
      721 gcgcggctca aagtcgcgac gaaatacgtg cagaccgcgc gcgagcactt cgcgaccaag
      781 ggcgtgcatg tcgatttgat caagctctac ggttccatgg agctcgcccc gctcaccggg
      841 ctcgccgatg cgattgtcga tctcgtgagt accggaaata cacttcgcgc caacaacctg
      901 aaggcggtgg aggagatcgc acccgtcagc gcccgtctcg ttgtcaacca ggcggcgctc
      961 aaactgaggc gcgctcaaat ccaaccgctg ctggacgcgt tcgcgcgggc cgtatcatga
     1021 cctcggtgcg ccgactcgac acgggccatc cagatttcga agcccagttg caggcgttgc
     1081 ttgccttcga gaacgcgcac gaccctgcgg tggagtcggc ggtcgcggga atcctcgagg
     1141 acgtgaaggc tcgcggcgac gaagcgctgc tggaatacac gcggcgctac gatcgcatga
     1201 gcgtgccgga tgctgcccgg ctcgagattc cgcgcccgga actgcagcgc gctctgaacg
     1261 cgctttctcc ggcgcagcgc gaagcgttgg cggcggccga gcggcgggtg cgcagctatc
     1321 acgagaagca gctcgcgcga tcctggagtt acgtggagcc ggacgggacc gagcttggcc
     1381 agaagatcac ggcactcgac cgtgtcgggc tgtacgttcc cggcggaaag gcggcgtatc
     1441 cctcctccgt catcatgaac gcggtgcccg cacgcgtcgc cggcgtgcgg gagatcatca
     1501 tgacggtgcc cacgccggac ggtgagcgca acgatctcgt gctggcggcc gcactgctgt
     1561 gccaggtgga tcgcgtgttc accgtcggcg gtgcacaggc cattgcggcg ctcgcgtacg
     1621 gcacggcaac gatccccgcc gtggacaaga tcgtggggcc gggtaacgct tacgtcgccg
     1681 ctgcgaagcg cagagtgttc ggcgtggtgg gcatcgacat gttggccggc ccttccgaaa
     1741 tcgttgtagt gtgcgacggg ggcaccgatc cggactggat cgccatggat ctcttctcgc
     1801 aggccgagca cgacgaactg gcacaggcga tcctgctgac acccgaccca cgattcatcg
     1861 atgccgttgc tgcgagcatc gaacgccagc ttgccgcgat gccgcggcgc gaggtgattc
     1921 gcgccgcact ccgatcgcgg ggcgcgctca tccgcgtgcg tgacctcgcc gatgcctgcg
     1981 cactggtaaa tcgcatcgcg cccgagcatc tcgagctttc ggtggccgat ccggatgcat
     2041 tgctgccgaa aatccgccac gccggcgcga tctttctcgg acgctacacc tccgaggcgc
     2101 tgggtgacta ttgcgcgggc cccaatcatg tgttgccgac ttcacgcacc gcgcggtttt
     2161 cttcgccgct cggtgtctac gacttccaga agcgcacgag cgtaatccgc gtctcgcgcg
     2221 aaggcgcgcg gcagctcggg gaaatcgccg ccgagctggc gcgcggcgaa gggctgtcgg
     2281 ctcatgcgct ggccgcagag taccggatga agggtccgta gcccgtggca aaacccgaaa
     2341 cgctcatacg cgacgccgtg ctggcgatga acgcctatca tgtgccctac agcgcgggca
     2401 tggtgaagct cgacgccatg gagaacccgt atacgctgcc ggccgcgctg cgcgaggaga
     2461 tcgcgaagct cgcgcaacag gccgcctaca atcgatatcc ggatgcccgg gcgacgcaat
     2521 tgcgtgtgcg gttgcgcgaa gtgatggggg tgccggacgc ggcggacata ctcgtcggca
     2581 acggatcaga tgaactgatc cagacgctca tgctggccac agcgcggccc ggtgcgctcg
     2641 tgctcggcgt ggagccgtcc ttcgtcatgt ttcgcctgat cgccgaattc tgcggcatgc
     2701 gattcctggg cgtgccgctc gacgccggtt tcgagctcga cgccgaacgc acggtagacg
     2761 cgattcgggc acaccggccg gcgctcgtca tcctcgccta ccccaacaat cccaccggga
     2821 acctctacga cgacgaagcg atggcgcgcg tcatcgcggc cgcgccgggc ttggttctcg
     2881 tggacgaggc gtaccatgca ttcgccgggc gcagctgcat gcggcagctc gccggcaatt
     2941 cgagcctcct cgtcatgcgg acgctctcca agctggggct cgcgggtctg cgcctgggga
     3001 tgctcgccgg cgcacccgag tggctcgagc atctcgataa ggtacgcttg ccgtataatg
     3061 taaacgtgct gactcaactc atcgcagagc gagtgctaca gcatcacgag gtgcttgcca
     3121 agcaggcggc gagcatccga gcggagcgaa cccgcgtgtt cgaagcgctg gcggcgctac
     3181 ccggtgtgca gccctaccgc agcgatgcca atttcattct ctttcgggt
//
LOCUS       DASURG010000294         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1175206, whole genome shotgun sequence.
ACCESSION   DASURG010000294 DASURG010000000
VERSION     DASURG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175206"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFY80_05110"
     CDS             complement(<1..161)
                     /locus_tag="VFY80_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX6007317.1"
                     /translation="MRILLLGLPGAGKGTQAQFLIEKYGIPQISTGDMLRNAVKAGTP
                     AGVEAKRYM"
     gene            complement(248..997)
                     /gene="kdsB"
                     /locus_tag="VFY80_05115"
     CDS             complement(248..997)
                     /gene="kdsB"
                     /locus_tag="VFY80_05115"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX6007318.1"
                     /translation="MDFVVVIPARYDSTRFPGKPLADIAGEPMVARVAERAAKSGARD
                     VLVATDHKAIAETVRACGYTAVMTRRSHASGTDRIAEVARRLRYPAHRIVVNVQGDEP
                     LIEPALIRAVASRLARERGAHIATACTPLRDAREFHDPNIVKAVLDNAGRALYFSRAP
                     IPHVRDAAGSMRGVPAYRHLGLYAYRCGFLKQFTKLKPAAIERAEALEQLRALAHGFR
                     IVATITRKSPYPGVDTPADLERVRSYFEQRA"
     gene            complement(998..1177)
                     /locus_tag="VFY80_05120"
     CDS             complement(998..1177)
                     /locus_tag="VFY80_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX6007319.1"
                     /translation="MDPKLLDILVCPLCKGPLVYRKAQAELLCKPCRLAYPIRDDIPV
                     MLEDEARKLAPEEEI"
     gene            complement(1167..>2016)
                     /gene="lpxK"
                     /locus_tag="VFY80_05125"
     CDS             complement(1167..>2016)
                     /gene="lpxK"
                     /locus_tag="VFY80_05125"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX6007320.1"
                     /translation="LTAGGTGKTPLVLWLSALLSAHGMHPGIVSRGYGGRRRAPAAVK
                     PESAAAEFGDEAVMLARLSGAPVWVGRDRAATARALLDAAPACDVIVSDDGLQHYRLE
                     RDLEIAVVDGKRGAGNGLLMPAGPLREPATRLKRVDAIVVNVSESATVGLKAIAPAAY
                     AMALRGSAFYNLLNPQHRAGAEHFRNRRVHAIAGIGHPERFFLHLTRLGIDFIAHPFP
                     DHHPYIPSELAYADAEYILMTEKDAVKCRDFAHEKHWVLPVSAELDPAFGELVLGKLR
                     RFRHGS"
BASE COUNT          346 a          650 c          671 g          349 t
ORIGIN      
        1 tccatgtatc gctttgcttc gactccggcc ggcgtgcctg ccttgacggc gttgcgaagc
       61 atgtcgccgg tggagatctg cggaatgccg tacttttcga tgaggaactg ggcctgcgtg
      121 cctttgccgg caccgggtag cccaaggagc agaatgcgca tgatactcag atgacgttca
      181 tcgggaggca acaagtcggc gggcaaagga gtatagcggg tttcgatgcg cccagaaacc
      241 gacgcggcta ggcgcgctgc tcgaagtaac tgcgcacgcg ctcaaggtct gccggcgtat
      301 cgacgcccgg atatggcgac ttgcgcgtga tggtcgctac aatgcgaaac ccatgcgcga
      361 gcgcacgcaa ctgctcgagc gcttcagcgc gctcgatcgc ggccggcttc aatttcgtga
      421 actgcttgag gaagccgcag cgataggcgt agaggccgag atggcggtat gcgggcacgc
      481 cgcgcatcga cccggccgcg tcgcgcacgt gcggaatcgg cgcgcgactg aagtagagcg
      541 cccgccccgc attgtcgagc acggccttta cgatgttcgg atcgtggaat tcgcgcgcat
      601 cgcgtagagg cgtgcacgcg gtggcgatat gcgcgccgcg ctcccgggcg aggcgcgatg
      661 ccaccgcgcg gatgagcgca ggctcgatca gcggctcatc gccctgcacg ttgaccacga
      721 tgcggtgcgc ggggtaacga agccgcctcg cgacttcggc gatgcggtcg gttcccgaag
      781 catgactgcg ccgcgtcatg acggcggtgt agccgcacgc acggacggtc tcggcaatgg
      841 ctttgtgatc ggtcgcgacc agcacgtcgc gcgcgccgct ttttgctgcg cgctccgcta
      901 cgcgggcaac catcggttcg ccggcgatgt cggcgagcgg cttgcccgga aaccgcgtgg
      961 aatcgtagcg ggccgggatg acgacgacga aatccactca gatttcttct tcgggcgcga
     1021 gctttcgcgc ttcgtcctcg agcatcaccg ggatgtcgtc gcggatggga taagcgagac
     1081 ggcacggttt gcacagcagt tcggcctgcg ccttgcggta gacgaggggc cccttgcaga
     1141 gcgggcagac cagtatgtcg agcagcttag gatccatgcc tgaatcgcct cagtttgccc
     1201 agcacgagtt cgccgaaggc cggatcgagt tcggcgctga ccggaagcac ccaatgtttc
     1261 tcgtgcgcga agtcgcggca ttttaccgca tccttttcgg tcatcaggat gtactccgcg
     1321 tcggcgtacg ccaactccga agggatatac gggtggtggt ccggaaacgg atgggcgatg
     1381 aaatcgatgc cgagccgggt gagatgcaga aaaaaacgct cgggatgtcc gatcccggca
     1441 atcgcgtgca cgcgccgatt gcgaaagtgc tccgccccgg cgcggtgctg cgggttgagc
     1501 aggttgtaga acgcactccc gcgcagggcc atggcatatg cggcgggcgc gatcgccttg
     1561 aggccgaccg tcgcggactc gcttacgttc accacgatcg cgtctacgcg cttcagccgg
     1621 gtggccggct cgcgcagcgg gccggccggc atgagcaagc cgtttcctgc accgcgcttt
     1681 ccgtctacca ctgcgatctc cagatcccgc tccaaccggt agtgctggag tccatcgtcg
     1741 ctcacgatca cgtcgcacgc aggcgcagca tcgagaagcg ctcgtgccgt cgccgcgcgg
     1801 tcccgtccca cccaaaccgg ggcgccgctc aagcgcgcca gcatgacggc ttcgtcgcca
     1861 aactccgccg ccgcgctctc cggcttgacg gcggccggcg cgcgccgcct gccgccgtaa
     1921 ccgcgactca caatcccggg atgcatccca tgagccgaga gcagcgcgga aagccagagc
     1981 acgagcggtg tctttcccgt tccacccgcc gtcaga
//
LOCUS       DASURG010000295         4776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_52008, whole genome shotgun sequence.
ACCESSION   DASURG010000295 DASURG010000000
VERSION     DASURG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52008"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..895
                     /locus_tag="VFY80_05130"
     CDS             158..895
                     /locus_tag="VFY80_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6007321.1"
                     /translation="MASTFSARALDIGLLVPINNTTMETELLAWLPQGSRCKTLRIPR
                     GPGTLGPSDLPAYIAQALRLAESFAADGTDLVIYGCTAAGFMMGPARDAELAQELARL
                     TGKSVVTTASAMIDALRNLGARNVALVTPYQDLVNERLRKFVEDCGIAVEVLASFRAR
                     NVEELGAITPEAIAELACQSMRDTCDALFIACSQLPTRDIVPRLEQAFGRPVWSSIRA
                     TAWRAAGAAQSVSLRGTKGEAAASASR"
     gene            complement(879..1508)
                     /locus_tag="VFY80_05135"
     CDS             complement(879..1508)
                     /locus_tag="VFY80_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6007322.1"
                     /translation="MLTDQSPVSRRRTAVELLIVIAAAATFAAAASDAASAAEPATVQ
                     DVEREVIPGADRMTSAERETYRRRMAAAATPEEKARIRAEYAAAASKPEASPRLVGDP
                     ARGAQLHRACFSCHGIERYTAPVTYVMASFLDSVLRASGLSDMPPSEPTRFKGKVRSV
                     SQLREFVARRNDYLNPKMTPQEIEDVVAYLNQTYYKFPNPQPDSVSATR"
     gene            2087..2986
                     /locus_tag="VFY80_05140"
     CDS             2087..2986
                     /locus_tag="VFY80_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX6007323.1"
                     /translation="MLPSHGRYRYSGIDTRPKYRWPDGKALALYVALNLEHFPFGAGG
                     GIDLDRETRPWSQRSWLWREYGNRVGGWRLASLFDELALPVGVLVNAENYDYCPELIA
                     RYRNRGDELIGHGISNGTQRPIDMTDVAEREMVREVTRRMSAADGMPPAGWLSPYLTP
                     SPVTTDLLVENGYEYVLDWGICDEQPFWVKGGQREILAIPYPIELNDQPAIVGRRHTA
                     GEYADMLVDQFDEMLRRSDEAPLVFGISLHSFIMGQPFRIVHLKRALKHMLLQRERIW
                     LTVPREIARYYRTLPREVQLVAE"
     gene            3004..3792
                     /locus_tag="VFY80_05145"
     CDS             3004..3792
                     /locus_tag="VFY80_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6007324.1"
                     /translation="MAKVAVSDGEIEYQDQGRGHPLIFVSGLGGVGRYWEPQLSAFST
                     RYRVVTYDQRGTGGSDRKQRTFSVDGMTSDLVGLMDALGIEKAHIVGLSTGGAIGQTL
                     AVEHPQRLARLVAVSTWTHCDPWFRRLFEARRVMYRQAGSELHAMFHPLWLYPPDYVN
                     THDAEIDEERSKAVAGAPPVEVSIGRIDAIMAFDRRKDLARITAPTLIVAADNDYITP
                     PYHAEALGRAISGSKLAIMKGGGHSVSKTRPEEFNRLVLDFLAG"
     gene            complement(3793..>4776)
                     /locus_tag="VFY80_05150"
     CDS             complement(3793..>4776)
                     /locus_tag="VFY80_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6007325.1"
                     /translation="CPETAVKIIFSPVISKAAWAIGESLLPEGFSIEVLSQDPERRTA
                     QLEEADFLMGFLRGMPLKADDYPHLKKVKLIQLLSAGYDGVDLERLRKSGIPLSNNGG
                     ANSYAVAEHAILLMLAVYRRLPALDRIVRAGQWKSSKLGEEQEHELAGKTVGIVGAGM
                     IGRTLAKRLMGFEVTLLYFDPIRLSGADEAKLAMSYRPLDDLLRASDVVTLHAPYNAS
                     TRHLVGDRTLGLMKRDAILINTARGELVDDDALYRALRDGRIWGAGLDAFHPEPPDPK
                     NPLLTLPNVVLSPHAAGPTWESWPKRFGNSYANIERVARGEPPLWVVPELR"
BASE COUNT          803 a         1527 c         1596 g          850 t
ORIGIN      
        1 cttgtgtccg gtggcggctt cgaactgcgg cgcgagcgca tccatgaccc cgcgcatgct
       61 gttcgaaccc atgacgttta ttgtgcccat cgccgcttcg cccgggttgg ccgggaagcg
      121 cctatactaa ccgctcgact catggtcggg gaatgccgtg gcatcgacat tcagcgcgcg
      181 tgcactcgat atcggacttc tggttcccat caacaatacg acgatggaaa cggagctgct
      241 cgcgtggctg cctcaaggca gccgctgcaa gacgttgcgc attccgcgcg gaccgggaac
      301 gctcgggccc tccgatctgc ccgcctatat cgcgcaggcg ctgaggctcg cggagtcatt
      361 tgccgcggac gggacggacc tcgtgatcta cgggtgcaca gcggcaggct ttatgatggg
      421 gccggcgcgc gacgccgaac tcgcgcagga gcttgcgcgc ctcaccggaa aatcggtggt
      481 gaccaccgcg agcgccatga tcgacgcgct gcgcaacctg ggcgcgcgaa acgtcgcctt
      541 ggtcactccg tatcaggacc tcgtgaacga gcggctgcgg aagtttgtgg aggattgcgg
      601 catcgcggtc gaggttcttg ccagtttccg cgcacgcaac gtcgaagagc ttggagcgat
      661 cacgccggag gcgatcgcgg agctggcgtg tcagtcgatg cgcgacacgt gcgacgcgct
      721 tttcatcgcg tgctctcaat tgcccacgcg cgacatcgtg ccccggctcg agcaggcgtt
      781 cgggcggccg gtgtggtcct ccatacgtgc gaccgcgtgg cgggcggcgg gcgcggcgca
      841 gtccgtgtcg ttgcgcggta cgaagggaga ggcagcggct agcgcgtcgc gctgacgctg
      901 tccggttgcg gattcgggaa cttgtagtag gtctgattga ggtacgcgac gacgtcctcg
      961 atttcctggg gcgtcatctt cggattgagg tagtcgttac ggcgcgccac gaattcccgc
     1021 agctgcgaca ctgaacgaac tttccccttg aagcgggtgg gctcggaggg cggcatgtcg
     1081 gacaggccgc tcgctctcag taccgagtcg aggaacgacg ccatcacgta cgtgaccggc
     1141 gcggtgtagc gctcgatgcc gtgacacgaa aagcatgccc ggtgcagctg cgcgccccgc
     1201 gcggggtcgc ccacgagccg gggcgaagcc tcgggcttac tggcggcggc cgcgtattcg
     1261 gcacgtatgc gcgccttctc ctccggcgtt gcggccgccg ccatgcgccg acggtaggtt
     1321 tcgcgctccg cgctggtcat gcggtccgcc ccgggtatca cctcccgctc cacatcctgt
     1381 accgttgcag gctccgcagc gctggccgca tcgctcgctg cggctgcgaa tgtcgccgcc
     1441 gccgcgatga cgatgagaag ctccacggca gtgcgccgac gcgataccgg tgattgatcg
     1501 gtaagcatgg tttctcccgc tgcagcacgc aggttgactt ggaaaaacta tacagatcgc
     1561 cgcgcggccc ggattgacct aagtcaaacg aaacgggatt gcgccggcag gcggcggcga
     1621 acaggtgcgg aactccagtc cgcggcgtgc ggactaaaag tgacccgagg agggggaggt
     1681 gccgcagcgg caggcggctg cgcccgaact gtcgtgtggg aatcatgcag atcaagagcc
     1741 aggtcgattt ctggggcggc gtccttttca ttgcgtttgg gctgctcgcg atcatcgtcg
     1801 cccgcgacta tccgatgggc agcgccatgc gcatggggcc ggggtacttc ccgacctaca
     1861 tcggcatcgt gctcgtcgcg ttcggcgcgt ggatcgccgt ggacggtttc aggaaggaag
     1921 gcgagcgcat cggcagtttt ccatggcgcg cgatcgttct cctgagcgtc gggttctcca
     1981 ggagccggtg taacgcgcga gcacatctgg acggcgtttc ccgtttgaca cgcggccgag
     2041 gagctgaaga caatacggct ccccgctctc cggattttca ggcgcgatgc ttccgtctca
     2101 cggccgctac cggtactccg gcatcgacac ccgtcccaag taccgctggc ccgacggcaa
     2161 ggcgctcgcg ctctacgttg cgctcaatct cgagcacttt ccgttcggcg cgggcggcgg
     2221 catcgatctc gaccgcgaga cgagaccgtg gagtcagcga agctggctat ggcgcgagta
     2281 cggcaaccgc gtcggcggct ggcggctggc gagcctcttc gacgagcttg cgttgccagt
     2341 cggcgtgctc gtcaatgcgg aaaactacga ttactgtccc gagctcatcg cgcgttaccg
     2401 caatcgtggc gacgagctga tcggccacgg tatatcgaac ggcacccaac gaccgatcga
     2461 catgactgac gtggccgagc gcgagatggt gcgcgaggtc acgcgccgca tgagcgccgc
     2521 ggacggcatg cctccggccg gttggctttc gccctatctc acgccgagcc ccgtgacgac
     2581 cgacctcctg gtggagaacg gctacgaata cgtgctcgac tggggcatct gcgatgagca
     2641 gccgttctgg gtgaagggtg ggcagcgcga aattctcgcg atcccgtacc cgatcgaatt
     2701 gaacgaccag ccggcgatcg tcgggcgccg ccatacggcg ggcgaatatg cggacatgct
     2761 ggtcgatcag ttcgacgaga tgctgcggcg ctccgacgag gcgccgctcg tcttcggcat
     2821 ctcgctccac agcttcatca tgggccagcc tttccgcatc gtgcacctga agcgcgcgct
     2881 caagcatatg ctgctccagc gcgaacgcat ctggctcacg gtgccgcgcg agatcgcgcg
     2941 ctattaccgc acgctgccgc gtgaagtgca gctcgtcgca gagtaacttc gagcggagga
     3001 gagatggcga aagtcgcagt atcggacggt gaaatcgagt accaggacca agggcgcggg
     3061 catccgctca tcttcgtgag cggcctcggc ggcgtgggac gctactggga gccccagctt
     3121 tccgcgttca gcacgcgcta tcgcgtcgtc acctacgacc agcgcggcac tggcggcagc
     3181 gacaggaagc agcgcacctt ttcggtggac ggcatgactt ccgatctcgt cggtctcatg
     3241 gacgcgctcg gcattgaaaa ggcccacatc gtggggctct ccacgggcgg cgcgatcggt
     3301 cagacgcttg ccgtcgagca cccgcagcgc cttgcgcggc tcgtcgccgt cagcacgtgg
     3361 acgcattgcg atccgtggtt ccgacggctc ttcgaagcgc gccgggtgat gtaccgccag
     3421 gcgggctccg agctgcacgc catgttccat ccgctgtggc tctatccgcc cgattacgtc
     3481 aatacgcatg acgcggagat agacgaggaa cgcagcaagg cggtcgcggg cgccccgccg
     3541 gttgaagtgt cgatcgggcg catcgacgcc atcatggcgt tcgatcggcg caaggacctc
     3601 gcccgcatca cggcgccgac gctgatcgtc gccgccgata acgactacat cacgccgccc
     3661 tatcacgccg aggcgctggg gcgggcgatc tcaggatcga agctcgcgat catgaaaggc
     3721 ggcgggcatt cggtctcgaa gacgcgaccc gaggaattca accgcctcgt gctcgacttt
     3781 cttgcgggat agctaccgca gctcgggcac aacccagagc ggcggctcgc cgcgtgcgac
     3841 gcgctcgatg ttggcgtagc tgttgccgaa gcgcttgggc cagctttccc acgtcggtcc
     3901 ggccgcgtgc ggcgagagca cgacgttcgg gagcgtgagc agcggattct tgggatccgg
     3961 cggttcggga tggaaggcgt cgagacccgc gccccatatt cgcccgtcgc gcagcgcgcg
     4021 gtatagcgca tcgtcgtcca ccagctcgcc gcgcgcggtg ttgatgagta tcgcatcgcg
     4081 cttcatcaag ccgagggtgc gatcgccgac gagatggcgc gtggaggcat tgtacggggc
     4141 gtgcagcgtc accacgtcgg acgcgcgtag aagatcgtcg agcggccggt atgacatcgc
     4201 gagcttcgcc tcgtcagcgc cgctcaagcg gatggggtcg aagtagagca gcgtgacttc
     4261 gaagcccatc aagcgctttg cgagcgtgcg cccgatcatc cctgcaccca cgatgccgac
     4321 agtcttgccc gcgagctcgt gctcctgctc ctcgccgagc ttgctcgact tccactgccc
     4381 ggcacggacg atacggtcga gagcggggag ccgccgatat accgcaagca tgagcaggat
     4441 cgcatgctcc gcgaccgcat acgagtttgc gccgccgttg ttggagagcg gaatgccgct
     4501 cttcctcagc cgctcgagat cgacaccgtc gtagcccgcg ctcaggagct ggatcagctt
     4561 gaccttcttc aggtgcggat aatcgtccgc cttgagcggc atgccgcgca ggaatcccat
     4621 gagaaaatcg gcctcctcga gctgcgcggt ccggcgctcg ggatcctgcg acaggacctc
     4681 gatgctgaag ccttcaggca acagcgattc gccgatggcc caggcggctt ttgaaatgac
     4741 gggcgagaaa atgatcttca cggcggtctc cgggca
//
LOCUS       DASURG010000296         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_665612, whole genome shotgun sequence.
ACCESSION   DASURG010000296 DASURG010000000
VERSION     DASURG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665612"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1257)
                     /locus_tag="VFY80_05155"
     CDS             complement(31..1257)
                     /locus_tag="VFY80_05155"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6007326.1"
                     /translation="MRVLLIGVGTVGEAIARISAQSDWCEAMILADFDLTRARTLADA
                     LGDAERFPAIQLDARDPATVADAARAHRADLVMNAVDPQFVMPIFRGALEADAHYMDM
                     AVSLSKPHPDRPFELPGVKLGDEQFALAHAWEARGKLALVGMGMDPGLTDVFAAHAAK
                     NDFDEVHEVHVRDGGDLEIPGYAFAPVFSIWTTIEECLNPPVIWSDGDWHTTEPFSAP
                     EDFPFPEGIGPVECVNVEHEEVLLVPRWLETSKVTFMYALGSDFIDKLKTIHALGMDR
                     TDTVAVKGVEVSPRDVLAAITPDPISLGDKFRGRAIVGTWVIGSKDARPRETFAYQMA
                     DAEEIWAKHHLQVVGWQTGFNPTIAMELLATNAWSGAGVLGPEAFDPDPYLALLDLHG
                     IHHATIEMDPGAHRPT"
     gene            complement(1396..>2058)
                     /locus_tag="VFY80_05160"
     CDS             complement(1396..>2058)
                     /locus_tag="VFY80_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007327.1"
                     /translation="PWQQAGSLLVGSMWVLPVLLGSILGVAITAGELERRTAHVSWSL
                     STDRRRWLLFRALPAGVFLGVVLGAGALGAELVTRTRLITDDAGFIDYQLRTALVPLR
                     GLLAFAIAIAVGAQLGRVLPALLIAIVVAAAATAGLGILVDAWHLGSAQFVSLARLVP
                     DQYPLIVRPGETQWPQGQGVMQIPISDFGKWLGIEAAAYFSVTAGIAIVADGIIAKRS
                     PR"
BASE COUNT          368 a          656 c          720 g          314 t
ORIGIN      
        1 tacctggctc cgtcgggcag acggcggcgt tcacgtcggg cggtgcgcgc cgggatccat
       61 ctcgatcgtc gcgtgatgga tgccgtgcag gtcgaggagc gcgaggtatg gatcggggtc
      121 gaacgcctcg gggcccagca ccccggcgcc ggaccaggcg ttcgtggcga gcagctccat
      181 ggcgatggtc ggattgaagc cggtctgcca gccgacgacc tgcaggtgat gcttggccca
      241 gatctcctcg gcatcggcca tctggtaggc gaacgtctcg cgcggccggg cgtccttgga
      301 gccgatcacc caggtgccga cgatcgcgcg accacggaac ttgtcaccga gtgagatggg
      361 atccggagtg atggcggcca gaacgtcgcg cggtgagacc tcgacgccct tgacggcgac
      421 cgtatcggtc cgatccatgc ccagcgcgtg gatggtcttc agcttgtcga tgaagtccga
      481 ccccaacgcg tacatgaacg tcaccttgct cgtctcgagc cagcgtggga cgagcaggac
      541 ctcctcgtgc tcgacgttga cgcactcgac cgggccgatg ccctccggga acggaaagtc
      601 ctcgggcgcg ctgaagggct cggtggtgtg ccagtcgcca tcggaccaga tgacgggcgg
      661 gttgaggcac tcctcgatcg tggtccagat gctgaacacc ggggcgaagg catagccggg
      721 gatctcgagg tcgcccccgt cgcggacgtg gacctcgtgg acctcgtcga agtcgttctt
      781 cgcggcgtgc gcggcgaaga catcggtcag gccggggtcc atgcccatgc cgaccagcgc
      841 cagcttgccg cgcgcctccc aggcgtgtgc aagagcgaac tgctcgtcgc cgagcttcac
      901 gccgggcagc tcgaagggcc ggtccggatg gggcttcgac aggctgaccg ccatgtccat
      961 gtagtgcgcg tcggcttcga gtgcaccacg aaagatcggc atgacgaact gcgggtcgac
     1021 cgcgttcatg accaggtccg ctctgtgggc gcgggcggcg tcggcgacgg tggccggatc
     1081 gcgggcgtcc agctggatgg ccgggaagcg ctcggcatcg ccaagggcgt cggcgagagt
     1141 gcgagcgcgg gtgaggtcga aatcggcgag gatcatcgcc tcgcaccagt ccgattgggc
     1201 ggagatgcgc gcgatcgcct cgcccaccgt cccgacaccg atcagcagga ctcgcatgga
     1261 tcaccagggt gcgtgttccg acgtggggca cgcgtctgcg caacaggatc tcgagtgccg
     1321 ggagcctacc gaatcagtgg aaaaggggcc tcatccgtgc tgcctcggtg gcgccgagcg
     1381 gatgataaca gcgactcagc gaggagagcg cttcgcgatg attccatcgg cgacgattgc
     1441 gatgccggct gtcacggaga agtatgcggc cgcctcgatg cccagccact tgccgaagtc
     1501 gctgatggga atctgcatca caccctggcc ctgcggccac tgcgtctctc ccgggcgcac
     1561 gatgagggga tattgatctg gaacgagccg cgcaagggag acgaattgcg cggatcccag
     1621 atgccaagca tcgaccagga tccctagccc tgcagtagcg gccgcagcga caacgatggc
     1681 gataagaagg gcgggaagca cgcgaccgag ctgcgctccc acggcgatcg caatggcaaa
     1741 cgccaagagt ccgcgcagcg gaactaacgc cgtgcgcaac tggtagtcga tgaagcctgc
     1801 gtcgtccgtg atcagccgag tccgcgtgac cagctcagcg ccgagcgccc cagcgcccag
     1861 cacgactccc aggaatacgc cagccggcag ggcccggaag agcagccagc gccgtcgatc
     1921 tgtgctgagt gaccaactga cgtgggcggt cctccgctcc agttcgccgg ccgtgatggc
     1981 gacgccgagg attgacccaa ggagcaccgg gaggacccac atggacccga cgagaagcga
     2041 accggcctgt tgccatgg
//
LOCUS       DASURG010000297         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1176006, whole genome shotgun sequence.
ACCESSION   DASURG010000297 DASURG010000000
VERSION     DASURG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176006"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..599
                     /locus_tag="VFY80_05165"
     CDS             180..599
                     /locus_tag="VFY80_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6007328.1"
                     /translation="MSDTELLPKSKPEPVRRLEVFTGSGRRRAWTAEQKTRIIAETHE
                     SGETVSAVARRHGLTPQQLFGWRRNARLRAEDGSGDRSAAFVPVIVEAARPGPAMPIA
                     PRSLVIEIVVGATTVRVPSGTDVETLQTVLRAVRAAT"
     gene            596..949
                     /gene="tnpB"
                     /locus_tag="VFY80_05170"
     CDS             596..949
                     /gene="tnpB"
                     /locus_tag="VFY80_05170"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX6007329.1"
                     /translation="MITVPAGIRVLVATKPVDFRRGADSLVALVREQLGHDPFSGTIF
                     VFRSKRADRLKIVAWDGSGLVLFWKRLEHGAFRWPPISDGVMRLTASQLAALVDGMDW
                     SRLQAIDVARPTATS"
     gene            1048..2607
                     /locus_tag="VFY80_05175"
     CDS             1048..2607
                     /locus_tag="VFY80_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX6007330.1"
                     /translation="MDIEALHALLAATRAERDAAIAERDQALSQIDRLRHLLRQLQRA
                     QFGRRSEKLDSDQLLLALEDIEQAIAANEAADDKKDAVAAKARAEKRRVNRGALPAHL
                     PRVDVTIEPEDMNCPCCREPMHVIGEETSKRLDVIPAQFRVIVTHRPKYACRSCEQAV
                     VQAPAPERLIKGGLPTEAMVAYVLVAKFAWHLPLYRQAQMLFAQGIDIKRAILAFWVG
                     YAAAELMPLYLRLRELILGSAKIAVDETVVPVLDPGRGCTKKGYFWAIARDDRPWGGT
                     DPPAVAYSYAPGRGAVHALKLLEHYRGVVQCDGYAAYKTVADAACGEGITLAFCWAHL
                     RRRFFDIAKDGPTPIASEALERIAKLYVIEKTIRGQSAGERRAVRQERSKPLVLALRA
                     WFEQQLARVSAKSLIAEAIRYALHHWDGLTRFLDDGRIELDTNIVERNIRPIVLNRKN
                     ALFAGHDQGAENWACIASLIETCKLHGVDPQAYFTDVLTKLVNLWPASRLDELMPWAW
                     AAEHATNKLAA"
BASE COUNT          491 a          848 c          916 g          499 t
ORIGIN      
        1 cggagatatc gcggcgacac agacgccgcg ctaagcgagt gacgcatggt gcgacaaaca
       61 tccgcctttg aacagttgcg gggctttatt gctgagcggt aagcggtctt tcgcccgcac
      121 attccacgcc gggagctgat cggcgaatgt ctgcccgctg agtcgagcgg gttagacaca
      181 tgtcggacac tgagcttttg cccaaatcta agccggagcc ggttcggcgg ctggaagtgt
      241 ttactgggtc cggtcggcgc cgcgcctgga ccgcagagca gaagacgcgg atcatcgctg
      301 agacccatga gagcggtgag accgtatcgg cggttgcgcg ccggcacgga ttgacgccgc
      361 agcagttgtt cggatggcgt cggaatgccc ggctcagggc agaagacggg agtggtgatc
      421 gcagcgcggc gtttgtgccg gtgattgtgg aagcggcccg gccgggcccg gccatgccga
      481 tcgcgccgag gtccctggtg attgagatcg tagtcggcgc cacgacggtt cgggttccgt
      541 cggggacgga tgtcgagaca ttgcagacgg tgctgcgcgc cgtgagagcg gcgacatgat
      601 tacggttccg gccggcatac gggtgctggt tgcgaccaag ccggtagact tccgccgcgg
      661 cgccgacagt ctcgtggctc tggtgcgcga gcagcttggg cacgatccat tctcggggac
      721 gatcttcgtc ttccgctcga agcgcgcgga ccggctgaaa atcgtggcct gggacggctc
      781 cggcctggtg ctgttctgga agcggcttga gcatggggca ttccgctggc cgccgatcag
      841 cgatggcgtg atgcgattga ccgcatcgca gctcgccgcg ctcgttgacg ggatggattg
      901 gtcgcggctg caggcgatcg acgttgcacg gccgacagcg acgtcgtgag cgctgcgacg
      961 aattgaatca ggtcgcacat tgtgtgtcac aatcgcgcgc gaatcaggtt ccgtgcgcgc
     1021 gtgagcgaga tcgcacctca gcgttctatc gatatcgaag cgctgcatgc gctccttgcg
     1081 gcaacgcgtg ccgagcgtga cgccgcgatc gccgagcggg atcaggcgct ttcgcaaatt
     1141 gatcggctgc ggcacctgct gcgccagttg cagcgcgcgc agttcgggcg gcgttcggag
     1201 aagcttgatt ctgaccagct gcttctggcg ctcgaagata tcgagcaagc cattgccgcg
     1261 aacgaagccg ccgatgacaa gaaggacgcg gtggctgcga aggcgcgcgc cgaaaagcgc
     1321 cgcgtcaatc gcggcgcgct tcccgcccat ttgccgcgcg tggacgtgac gatcgagccc
     1381 gaagacatga actgcccgtg ctgccgggag cccatgcatg tgatcggtga ggagacctcc
     1441 aagcggctcg acgtgatccc ggcgcagttc cgggtgatcg tgacgcaccg tcccaaatat
     1501 gcctgccgga gctgcgagca ggcggtggtt caggcgccgg cgcccgaacg gctgatcaag
     1561 ggtgggctgc cgaccgaggc gatggtggcc tatgtcctgg tcgccaaatt cgcctggcat
     1621 ctgccgctct accggcaagc gcaaatgctg tttgcccaag gcatcgacat caagcgtgcg
     1681 atcttggcgt tctgggtcgg ctatgccgcg gccgagctca tgccgctcta ccttcggctg
     1741 cgcgaactca tcctcggctc cgccaagatt gcggtcgatg agaccgtggt gccagtgctc
     1801 gatcccggcc gcgggtgcac caagaaagga tacttctggg ccattgcccg ggacgatcga
     1861 ccctggggcg gcactgatcc gccggccgtc gcctacagct acgctcccgg ccgcggcgcg
     1921 gtccatgcgc tcaagctgct cgagcactat cgcggcgtcg tgcaatgcga cggatatgcg
     1981 gcctacaaga ccgttgccga cgcagcatgc ggcgagggga tcacgctcgc cttctgttgg
     2041 gcccatctgc ggcgccggtt cttcgacatc gccaaggacg gccccactcc gatcgccagc
     2101 gaagcgcttg agcgcatcgc gaagctctat gtgattgaga agacgatccg cgggcagagt
     2161 gccggcgagc gccgcgccgt gcgccaggaa cgaagcaagc cgctcgtgct ggcactcaga
     2221 gcgtggttcg agcaacagct ggcgcgcgtc tcggccaagt cgctgatcgc cgaggccatc
     2281 cgctatgcct tgcaccattg ggacgggctc actcgtttcc tcgacgacgg ccgcatcgag
     2341 ctcgacacca acatcgtcga aagaaacatt cgtcccatcg tgttgaatcg gaaaaacgca
     2401 ctcttcgccg gccacgatca gggagcggag aactgggcct gtatcgcgtc gctcatcgag
     2461 acctgcaaat tgcatggtgt cgatccgcag gcctacttca ctgacgtgct caccaagctg
     2521 gtcaatctct ggcccgcctc gcgcctcgat gaactcatgc cctgggcctg ggcggccgag
     2581 cacgccacca acaagctcgc ggcgtgatcc accgaatcgc gccgcaaccc gcgcgaaaaa
     2641 atcaaggcgt caaagtcgtg cggtcagaag accgcttgcg aatgaccttg gattgatcaa
     2701 tggaatgttc gttactctcg aggaaatcgt cgatgagggc agcctgttct tctc
//
LOCUS       DASURG010000298         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1176223, whole genome shotgun sequence.
ACCESSION   DASURG010000298 DASURG010000000
VERSION     DASURG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176223"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFY80_05180"
     CDS             <1..467
                     /locus_tag="VFY80_05180"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX6007331.1"
                     /translation="EIDLEWRFEETVMGKYRHWIFSLGALFISGTAHSAAPAMKEGLW
                     EVTAQVEEPKGSGAKPTTVRHCMTQKDLQNPQKIVPGGNGECEIRDHKVQGNTVSWAM
                     LCKGKTALSGTGSITFAETTYTGSSTLAVNRGERTEKMIVRYAGKHVGPCKK"
     gene            complement(477..1001)
                     /locus_tag="VFY80_05185"
     CDS             complement(477..1001)
                     /locus_tag="VFY80_05185"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEX6007332.1"
                     /translation="MRARLLLIAACLMPLTAVAQWRDFEADFDEGRKAWKEIEAQIPA
                     YPKPENLILFETAPAGHQFYVDAPSVSVGDDGVVRYTLFVKTAGGAVNVSFEGIRCET
                     REQKYYAVGRRDGTWMRARDPVWKRIEVREFNRPHQVLYADYFCDGKAPVANTRAAVD
                     ALRYGSNHPRYKIQ"
     gene            complement(991..2271)
                     /locus_tag="VFY80_05190"
     CDS             complement(991..2271)
                     /locus_tag="VFY80_05190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6007333.1"
                     /translation="MTTSKQARSDGVTQAQEETPKRLPEMDTQLINRFERRIRELGLP
                     LGVRLWNGNAITPAVEPRVTIALRTPKVLLSLLNPSMGKLARHYVEQELDVEGEARDV
                     VRIAEELSGARGNTGSGSSRLFRWISHTRGFDRKAIRHHYDVGDDFFKLWLDRRRVYS
                     CAYFKRADDNLEIAQEQKLDLICRKLQLQPGERFLDVGCGWGALVMWAARHYKVRATG
                     ITLSQNQHDYAQARIREEGLEDTCEVQLLDYRDVPEKDPFDKIASVGMLEHVGRSNLP
                     LYFGKLFRLLKPGGLVMNHGITLNVNGASQLGSGIGEFIDEYVFPGGELVHVSQLIAD
                     MSEQGLECRDVESLRPHYAKTLWHWVERLEANREAAIAAVGEKIYRVWRIYMAGSANS
                     FERGWLSIHQVVAGRPHADGTLPLPLSRDYIYAS"
     gene            complement(2348..>2894)
                     /locus_tag="VFY80_05195"
     CDS             complement(2348..>2894)
                     /locus_tag="VFY80_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX6007334.1"
                     /translation="AYAKARNEFRARVMAHKRDRTVALGEHVTLIFEDELTIRYQIQE
                     MLRAERIFEEAGIQEELDAYNPLVPNGSNWKATMMIEYPDVEERHRMLAKLVGIEDKV
                     WVKVESHPRVYAVADEDLERETATKTSSVHFLRFELDASMVQALKRGAGLSMGIDHPQ
                     YNVTVDDVKAPVRDALAADLV"
BASE COUNT          523 a          921 c          917 g          533 t
ORIGIN      
        1 cggagataga cctggaatgg cgatttgagg agaccgtgat gggtaaatat cggcactgga
       61 tcttcagcct tggtgcgctc ttcatctccg gtacggcgca ttcggcggcg cccgcgatga
      121 aggaaggcct gtgggaggta acggcccaag tggaggaacc gaaaggaagc ggcgccaagc
      181 cgactaccgt gcggcactgc atgacgcaga aggacctgca gaatccgcaa aagatcgtcc
      241 ccggcggcaa cggcgagtgc gagatccgcg accacaaggt gcagggtaac acggtgagct
      301 gggcgatgct gtgcaagggc aagacggcgc tgagcggcac cggcagcatc acgttcgccg
      361 agacgaccta cacggggagc agcacgctcg ccgtcaatcg tggagagcga acggagaaga
      421 tgatcgtgcg ttacgcgggc aagcacgtcg gcccgtgcaa gaagtgatgc gatcgcttac
      481 tgaatcttgt atcgcgggtg attggagccg tacctgagcg catcgactgc ggcgcgcgta
      541 ttggccaccg gcgccttgcc gtcgcagaaa tagtcggcat acagaacctg gtgcggccgg
      601 ttgaactccc ggacctcgat gcgcttccac accggatcgc gcgccctcat ccacgtcccg
      661 tcgcgacgac cgaccgcgta gtacttctgc tcgcgggtct cacagcggat gccctcgaag
      721 ctcacgttga ctgcgccgcc cgccgttttc acgaacagcg tatagcgcac cacaccgtcg
      781 tcgcccactg aaaccgacgg ggcatcgacg tagaactggt gtcccgcggg cgccgtctcg
      841 aagaggatga gattctccgg cttgggatag gcggggatct gcgcctcgat ttccttccac
      901 gctttcctgc cttcgtcgaa gtccgcctcg aaatcgcgcc actgagcgac ggcagtgaga
      961 ggcatgaggc acgccgcgat cagcagcagc ctagctcgca tagatgtagt cgcgcgagag
     1021 cggcagcggg agtgtcccgt cggcgtgcgg ccgtcccgcc acgacctggt ggatggagag
     1081 ccagccgcgc tcgaaggagt tggccgagcc cgccatatag atgcgccaga cgcggtagat
     1141 cttctcgccc acggcggcga tcgcggcctc gcgattcgcc tccagccgct caacccagtg
     1201 ccacaaggtc ttcgcgtagt gcgggcgcag actctcgaca tcacggcact cgagcccctg
     1261 ctcggacatg tcggcgatga gctgcgagac atgcacgagc tctccgccgg ggaaaacgta
     1321 ttcgtcgatg aattcaccga tgccgctccc cagttgactc gcaccgttga cgttgagcgt
     1381 gatcccgtgg ttcatgacaa gcccacccgg cttgagcagc cggaagagct tgccgaagta
     1441 aagcggaaga ttgctgcggc ccacgtgttc cagcatgccg acgctcgcga tcttgtcgaa
     1501 cggatccttc tccggaacgt cgcgataatc gaggagctgc acctcgcagg tgtcttcgag
     1561 cccttcttcc cggatgcgcg cctgggcata gtcgtgctgg ttctggctca gcgtgatgcc
     1621 ggtcgcgcgc accttgtagt gccgcgccgc ccacatgacg agcgcgcccc agccgcagcc
     1681 gacatcgaga aaccgctcgc cgggctgaag ctggagcttt cgacagatca gatcgagctt
     1741 ctgctcctgc gcaatctcga ggttgtcgtc cgcacgcttg aagtaggcgc aggaatacac
     1801 gcggcggcga tcgagccaca gcttgaagaa gtcgtcgccc acatcgtaat gatgccggat
     1861 cgccttgcga tcgaagccgc gcgtgtggct aatccatctg aagagccgcg agctgccgct
     1921 gcccgtgttc ccgcgggcgc cgctcaactc ctcggcgatg cgcacgacat cccgcgcctc
     1981 gccttccacg tcgagttcct gttcgacgta gtggcgggcg agcttgccca tgctggggtt
     2041 gagcagcgac agcagcacct tgggggtgcg gagcgcaatc gtcacgcgcg gttcgaccgc
     2101 cggcgtgatg gcattgccgt tccacagtcg cacgccgagc ggcagcccaa gctcccggat
     2161 gcggcgttcg aaacggttga tgagttgcgt gtccatctcg ggcaagcgct tgggcgtctc
     2221 ctcctgagcc tgcgtgacgc cgtcactccg agcctgcttt gacgtcgtca ttgcggacga
     2281 tgatcctgaa tccagacgct tttctggatc ccggcgtttg ccgggtgcgt tacaaccggg
     2341 atccggttca gaccagatca gcggcgagcg cgtcgcgaac cggcgccttc acatcgtcaa
     2401 ccgttacatt atattgcgga tggtcgattc ccatcgagag gccggcaccg cgcttgagcg
     2461 cctgcaccat cgacgcgtcc agctcgaagc gcaggaagtg caccgaggag gtcttcgtgg
     2521 cggtctcgcg ctcgagatcc tcgtcggcga cggcgtagac gcgcggatgg ctctcgacct
     2581 tcacccagac cttgtcctcg atgccaacga gcttcgcgag catgcgatgc cgctcttcga
     2641 cgtccggata ttcgatcatc atggtcgcct tccagttgct gccattcggg acgagcggat
     2701 tataggcgtc gagctcctct tggatgccgg cttcctcgaa tatgcgctcg gcgcgcaaca
     2761 tctcctggat ctgatagcgg atcgtgagct cgtcctcgaa gatgagcgtc acgtgctcgc
     2821 ccaaggccac cgtgcggtcg cgcttgtgcg ccatgacgcg ggcgcggaac tcgttccgcg
     2881 ccttggcgta ggct
//
LOCUS       DASURG010000299         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_563498, whole genome shotgun sequence.
ACCESSION   DASURG010000299 DASURG010000000
VERSION     DASURG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563498"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFY80_05200"
     CDS             complement(<1..872)
                     /locus_tag="VFY80_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6007335.1"
                     /translation="MLHANPNRSGELERNVAVALRFKKSQGTPEMPLVEKEVLAPHYD
                     RVRGGNLHLAANARDEEFTHPGLYLRKALPDRVDVIERVIADGDRVGLLFRVTATHTG
                     GFFGVPPSGKRIDVYEIAMLKLAAGQMIEGWFMMDEAALLRDMGATMPKRRDGRIVAP
                     AVPDGGEEVDACIARLKAKGSLSRQDRNKIAAVTTRARHGGDMRHADHRHLRYGFAHL
                     RQYCTARGIPFDLDAAIPDRTDRIEVLMAEGDEVWMRFSTSGTHRGPLAGIGPTGKRV
                     GVHVVALMRFAEEK"
     gene            1123..1302
                     /locus_tag="VFY80_05205"
     CDS             1123..1302
                     /locus_tag="VFY80_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX6007336.1"
                     /translation="MPFRKFVCQVCGHVYDEAAGDPVAGIAPGTRWEALPADWTCPEC
                     GAAKADFLPAEEAAA"
     gene            complement(1331..2311)
                     /locus_tag="VFY80_05210"
     CDS             complement(1331..2311)
                     /locus_tag="VFY80_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007337.1"
                     /translation="MRAILGVSLYAAVAVGSLGVPATSPAQNFPSKTVRFVITYPMGG
                     GSELTVRPIAQKLGERWGQQVIVESRPGGSAMIGTDFVVRSPPDGHTLLLSASSEVSM
                     NVVLFRKMPYDPVQDLQPVTLVTRTAPVLLAHPSLPVKSMKELIALARAKPGALNYAT
                     IGAGTPQHFAGELMKRTFRIEMTHVPYKGAAPAIIDLLGGHVPIGLTAPTSAMPHIQA
                     GRLRALAVTGAQRLGALPDVPTIAESGAPGFDIGQWFAIWLPAKTPKDIAGKIHSDIV
                     AIVQSSEYRKRQLELASDAVGSDPEALRTTQKADIEKYRKLAAAIGITPE"
BASE COUNT          572 a          954 c         1001 g          609 t
ORIGIN      
        1 atcttttcct cggcgaaccg catgagcgcg acgacgtgca cccccacgcg cttgccggtg
       61 gggccgattc cggcgagcgg gccgcggtgg gtgccgctgg tggaaaagcg catccacact
      121 tcgtcgcctt ccgccatcaa cacttcgata cggtcggtgc ggtccggtat cgcggcgtcg
      181 agatcgaatg gaatgccgcg cgcggtgcaa tactggcgca gatgcgcaaa gccatagcgc
      241 aggtggcggt gatccgcatg ccgcatatct ccgccgtgtc gtgcacgggt cgtcacggcc
      301 gcgatcttgt tgcgatcctg acgggaaaga ctgcccttgg ccttaaggcg cgcgatacag
      361 gcgtccacct cttcgccccc gtcaggcacg gccggcgcga cgatgcgacc gtcccttcgc
      421 ttcggcatag tcgcgcccat atcgcgcagg agcgcggcct catccatcat gaaccagcct
      481 tcgatcatct ggccggctgc gagcttgagc atcgcgatct cgtagacgtc gatgcgcttg
      541 ccgctcggcg gaacgccgaa gaagccgccg gtgtgcgtgg cggtgacgcg gaagagcagg
      601 cccacgcggt cgccgtccgc aatgacgcgc tcgatgacgt ccactcgatc cggcagcgcc
      661 ttgcggagat agagccccgg gtgcgtaaac tcttcatctc gggcatttgc ggccagatgg
      721 aggttgccgc cgcgcacacg gtcgtagtgg ggcgcaagca cctccttttc gaccagcggc
      781 atttcgggcg tgccttgcga tttcttgaac cgcaacgcga cggcgacgtt gcgctcgagt
      841 tctccggatc gattggggtt cgcgtgcagc atgatgaggt ctggattgaa aatgggcgca
      901 tagtttatcc ggtttccgga acgcaaacgc gctttgcatc atccaatgcc gcatccgaac
      961 ttcggagaca gcgacgaagc ccgctctgca ccagcctgcc ctcatcgcaa cgccgaaacg
     1021 gtttgcgcgc cgcagcgcgc ggcatgtggg tgcacgatgg tgcgacgcgc tcgcgctcgc
     1081 gctcacgcgc tcggcgagag cgattgacta aaggagaacg aaatgccttt tcgaaaattt
     1141 gtctgccagg tgtgcgggca tgtctacgac gaagccgccg gggatcctgt tgcgggaatt
     1201 gcgccaggca cgcgctggga agcgctcccg gccgactgga cctgccccga gtgcggcgca
     1261 gcgaaagcgg acttcctgcc tgcggaagag gccgcggcct gaaggaaagc cgcgcccgcg
     1321 cgggaaagcg ttattccggc gtgatgccga tcgcagccgc gagcttgcga tatttttcga
     1381 tatccgcctt ctgcgtggtg cgcaacgctt caggatcgga gcctaccgcg tcgctcgcaa
     1441 gttcgagctg gcgtttcctg tattccgaag attgaacgat cgcaacgatg tccgagtgaa
     1501 tcttcccggc gatgtccttc ggcgtcttgg ccgggagcca gatcgcgaac cactgaccga
     1561 tatcgaatcc cggcgccccc gattcggcga tggtcggcac atcgggcagc gcaccgaggc
     1621 gttgcgcgcc cgttaccgcc agtgcgcgga gccgccctgc ctggatatgg ggcatcgccg
     1681 aagtgggtgc cgtgaggccg atcgggacat ggcccccgag aagatcgatg atcgcgggcg
     1741 ccgcgccctt atacggcacg tgcgtcatct cgatgcggaa ggtcctcttc atgagttcgc
     1801 cggcaaagtg ctgcggcgtt ccggcgccga tcgtcgcgta gttgagcgca ccgggcttcg
     1861 cgcgcgcgag tgcgatcagc tccttcatcg atttcaccgg cagcgatgga tgtgcgagca
     1921 gcaccggggc ggttcgcgtg acgagcgtca ccggctggag atcctgcacg ggatcgtagg
     1981 gcatcttcct gaagagcacc acgttcatcg acacttcgct cgaggcgctg agaagcagcg
     2041 tgtgtccgtc cggcgggctt ctcacgacga aatcggtacc gatcatcgcg ctgcctcccg
     2101 ggcggctttc gacgatcacc tgctgtcccc agcgctcgcc gagcttctga gcaatcggcc
     2161 gcacggtcag ctcggagccg ccgcccatgg ggtaggtgat gacgaaccgg accgtcttgc
     2221 tgggaaagtt ctgtgcgggg ctcgtggccg ggaccccgag tgagcccacg gcaaccgctg
     2281 cgtaaagcga gactcccaag attgctctca tcggggttcc tcctttttga gcctgacccg
     2341 attgttcgaa ccgttctgaa cttgaattac aggctcaatt taccacgatg ccagagggaa
     2401 gagatgacgg aggagaagat cgcttttgcg ctgaagcagg cgaagctaca cactggtggc
     2461 gctgcattca gagtgaatgc aacgctcagg taatacaact aatccttttt ccactctcca
     2521 agccaatatt gcttcgatgt aaggcgatgc tgccttctga tgacggtgct gatgaccgca
     2581 tcttcgatgt cccgagacga caatcggttg ggcttctttt cgagtgcgag tatcactcga
     2641 cgatagagag aatcatggcg caggttctcg agcagtaatc gcgccgccgg ttcacaccgc
     2701 tcctgcacca gcgcgacacg cgagtgccat tgccttacgg caggcattta cgggactccc
     2761 ctgagcgtat ttcttatgcg ggcgttggta gcaagttccg ggccgacatt atgataaatg
     2821 aatggaatca agaagggcag cctggcgagg atcaggcctc acgccgcctc tgtaaagaag
     2881 gccgacattt cggttcttac cattcccgcg gggcatcggc ctccagaatg gacatccccg
     2941 gtgtgacgcc cgggcttgaa ccgcggcgaa ccccgcgtag cggggtccgt agagcaaact
     3001 tctagtaacc gtcgtatcga tatatatagg ttcgcggtgc tggggccggg gcataatagg
     3061 tatggcccgc cggtggcacg taatgagtgt gaggcggcgc ctcgtagtgc gagtgaggtg
     3121 ccggcgccgg ataagc
//
LOCUS       DASURG010000300         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1073984, whole genome shotgun sequence.
ACCESSION   DASURG010000300 DASURG010000000
VERSION     DASURG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073984"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFY80_05215"
     CDS             complement(<1..932)
                     /locus_tag="VFY80_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6007338.1"
                     /translation="MVKSLDRKLLRDLWSLKTQVVSIALVIACGIGGFIGSFSTYGSL
                     LWSRDNYYATARFPHVFATAKRAPAWLLERIRGVAGVVEVEARVVRDAQLSIPGVVPP
                     MIARLIGEDFRHPSPVNRLTLRSGRWPAPGARGEVVVNQRFLEARGLELGDGMSVLMN
                     GKLEHVTLVGTALSPEYIFATRGGGMPDDEWFAVLWTDATQLAAAFNMEGAFNSVLLR
                     LSKNTSYEKTVEQLDSILEPYGGFGAVGREDQVSHKILSQEINQQRVFGTVLPAIFLL
                     VAAFILNVVLHRQVNAQRGEIAALKALGYEGAWIG"
     gene            complement(926..1585)
                     /locus_tag="VFY80_05220"
     CDS             complement(926..1585)
                     /locus_tag="VFY80_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6007339.1"
                     /translation="MGEVSVHALQGVDVTVAAGEFIVLLGPSGSGKSTLLNILGGLDT
                     PTSGEVRFLDQDLTRADEPTLTRYRRQHVGFVFQFYNLIPSLTARENVALVTDIAEKP
                     MPAAQALAWVGLAERLDHFPSQLSGGEQQRVAIARAIVKRPDVLLCDEPTGALDYETG
                     KLVLEVIARINGELGTTAMVITHNAAIAGIADRVLRLGDGKIVGVEKPARKLTPAEIS
                     W"
     gene            complement(1655..1975)
                     /locus_tag="VFY80_05225"
     CDS             complement(1655..1975)
                     /locus_tag="VFY80_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007340.1"
                     /translation="MMRPIWILWPSFVVAGIAEAVFFTLFDPADLHMFGEPFAASRMA
                     VYTLGFFAFWGFAAASSAFTCFLQRSAADINRMCPLEPTSRPPGCPKRVEPEQVVQES
                     GSSR"
     gene            complement(1988..2482)
                     /locus_tag="VFY80_05230"
     CDS             complement(1988..2482)
                     /locus_tag="VFY80_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX6007341.1"
                     /translation="MTSVDAKPWYREPWPWLLMSGPAVVVFAALVTLWLALDGNDALV
                     ADDYYKRGLAINQVLARDHAAAARGYVAKVTFDPASAHVRVALTGKDVPAALLLTLVH
                     PTRGGSDRIVPLAQVAAGLYEAPLELPSAGRWRIVLEDAARTWRLSADARLPHDTVLA
                     LRAH"
     gene            complement(2534..3931)
                     /gene="ccoG"
                     /locus_tag="VFY80_05235"
     CDS             complement(2534..3931)
                     /gene="ccoG"
                     /locus_tag="VFY80_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX6007342.1"
                     /translation="MNGARSGENVDAGLYEIRQKIYPRAVHGWFATWRVLLVVATQLV
                     FYGLPWLTWNGRQAVLFDLGARKFYIFGYVFWPQDFIFLTALLVIAALALFLFTAVAG
                     RLWCGYACPQTVYTEIFMWIERVVEGDRVQRMRLDRSPAGARKLTLKSAKHALWIAFA
                     LWTGITFVGYFTPIHVLMGEIATATLGPWETFWILFYGFATYGNAGWMREQVCKYMCP
                     YARFQGVMFDRDTLIITYDAGRGEPRGGRSRATDYRAAGLGSCVDCNICVQVCPTGID
                     IRNGLQYECIGCAACIDGCDQVMDRMGYPRGLIRYTTENEMRAGAAFAPLRRLLRPRV
                     AAYSALLVLVCAALAAGLYLRTPLKVDVIRDRAALVRETNEGLIENVYRLQIMNTDER
                     TRRYTVGASGLEGLNVVVRQPVEVPGATTVAVAIAVQIDPANLRPGSYGVVFHVADME
                     DPHIRADEKSRFFVR"
BASE COUNT          721 a         1327 c         1287 g          603 t
ORIGIN      
        1 ccgatccagg cgccctcgta gcccagggcc ttcagcgcgg cgatctcccc gcgctgcgca
       61 ttgacctgcc ggtggagcac gacgttcagg atgaaggcgg cgaccagcag gaagatggcg
      121 ggcagcaccg tgccgaacac gcgctgctgg ttgatctcct ggctgaggat cttgtgcgag
      181 acctggtcct cgcggcccac ggcgccgaag ccgccgtagg gttccaggat cgaatccagc
      241 tgctccacgg tcttctcata ggacgtgttc ttcgaaaggc gaagaagcac cgagttgaac
      301 gcgccttcca tgttgaacgc ggccgcgagc tgcgtggcgt cggtccacag caccgcgaac
      361 cactcgtcgt ccggcatgcc gccgccgcgg gtggcgaaga tgtactcggg actgagcgcg
      421 gtgcccacca gcgtcacgtg ctctagcttg ccgttcatca gcacgctcat gccgtcgccc
      481 agctcgagcc cgcgcgcctc gaggaagcgc tggttgacca ccacctcgcc gcgcgcgccc
      541 ggcgccggcc agcggccgct tcgcagcgtc aggcggttca ccggcgacgg atggcggaag
      601 tcctcgccga tgaggcgcgc gatcatcggc ggcactacac ccggaatgga cagctgcgcg
      661 tcgcgtacca cccgcgcctc gacctccacg acgccagcga cccctcgaat cctttcgagc
      721 agccacgccg gggcgcgctt ggcggtggcg aaaacatgcg ggaagcgcgc ggtggcgtaa
      781 tagttgtcgc gcgaccacag cagcgagccg tacgtcgaga acgacccgat gaagccgccg
      841 atgccgcagg cgatcacgag cgcgatcgac accacctgcg tcttcagcga ccacaggtcg
      901 cgcagcagct tgcggtcgag gcttttcacc acgagatctc cgcgggcgtg agcttgcgcg
      961 cgggcttttc cacaccgacg attttcccgt cgcccaggcg cagcacccgg tcggcgatgc
     1021 cggcgatggc ggcgttgtgg gtgatgacca tggcggtcgt accgagctcg ccgttgatgc
     1081 gcgcgatgac ttcgagcacg agcttcccgg tctcgtagtc gagcgcgccg gtcggctcgt
     1141 cgcaaaggag cacgtcgggg cgcttgacga tggcgcgcgc gatcgccacc cgctgctgct
     1201 ctccgcccga gagctgcgac ggaaaatgat cgagccgctc cgcgaggccg acccacgcga
     1261 gcgcctgggc cgccggcatc ggcttctcgg cgatgtcggt gacgagcgcc acgttctcgc
     1321 gcgcggtcag gctcgggatc aggttgtaga actggaagac gaagccgaca tgctggcggc
     1381 gatagcgggt gagcgtcggc tcgtcggctc gggtgagatc ctgatcgaga aagcgcactt
     1441 ctccggaagt cggcgtatcg agcccgccca gaatgttgag cagcgtgctc ttgccgctgc
     1501 ccgagggccc cagcagcacg atgaattcgc ccgccgccac ggtgacgtcc acgccctgca
     1561 gcgcgtgcac gctcacctca cccatgcgat agatcttggt gatgcggcgt gcgctaaaca
     1621 cctgtctctg gaaagcggaa ggcgtcaacc ggcctcaccg tgacgatccg ctttcctgca
     1681 cgacctgctc cggctctacc cgtttcgggc agcccggcgg gcgggacgtc ggctcgagcg
     1741 ggcacatccg attgatatcc gcggcgcttc gttgcagaaa gcaggtaaac gcgctggaag
     1801 cggcggcgaa cccccagaac gcgaaaaacc ctagcgtgta caccgccatg cggctcgcgg
     1861 cgaacggctc gccgaacatg tgcaaatccg ccggatcgaa cagcgtaaag aacaccgctt
     1921 cggcaatgcc ggcgacgacg aaggaaggcc acaggatcca gatcggtcgc atcatatgga
     1981 agccctttca atgcgcacgc agtgcgagca ccgtgtcgtg cggcaggcgc gcatccgcgc
     2041 ttaaccgcca ggtcctcgcc gcgtcttcga gaacgatccg ccagcgcccc gcagagggca
     2101 gctcgagcgg cgcttcgtag aggccggcgg ccacctgcgc gagcggcacg atccgatcgg
     2161 acccgccacg ggtgggatgg accaacgtaa gaagcagcgc ggccggcaca tccttaccgg
     2221 tcaaggccac gcgcacgtgc gccgaagcag ggtcgaacgt caccttcgcc acataaccgc
     2281 gtgccgcggc cgcatgatcc cgcgcgagga cctggttgat cgcgagtccg cgcttgtaat
     2341 aatcgtcggc gaccagggcg tcgttgccat cgagcgcgag ccacagcgtg acgagtgcgg
     2401 cgaagacgac cacggccggc ccggacatga gcagccacgg ccacggctca cggtaccagg
     2461 gctttgcatc gacggacgtc atttcacggt tcccggctcc ggttcaagag ggcgtattct
     2521 aagcatgtcg tcttcaacgc acgaagaagc gcgatttttc gtcggcacgg atatgcggat
     2581 cctccatgtc ggcgacatga aagacgacgc catacgaccc gggcctcaga ttggccggat
     2641 cgatttgcac cgcgatggcg acggccactg tggtggcgcc cggcacttcg accggctggc
     2701 gcacgacgac gttcaacccc tcgagaccgc tcgcccctac cgtgtagcgg cgcgtgcgct
     2761 cgtccgtgtt catgatctgc aggcgataga cgttctcgat caaaccctcg ttcgtctcgc
     2821 gcacgagcgc ggcgcgatcg cgaatgacat ccaccttgag cggcgtgcgc aggtacaggc
     2881 ccgctgcaag cgccgcgcat acgaggacga ggagcgcgct gtacgccgcc acgcgggggc
     2941 gcagcagccg gcgcagcggg gcgaatgcgg cgcctgcgcg catctcgttt tcggtcgtgt
     3001 aacggatgag gccgcgcggg tagcccatgc ggtccatcac ctggtcgcaa ccgtcgatgc
     3061 acgcggcaca gccgatgcac tcatactgca ggccgttgcg gatgtcgata cccgtggggc
     3121 agacttgcac gcagatattg cagtccacgc aggagccgag cccggccgcc ctgtagtccg
     3181 tcgcgcggct gcggcccccg cgcggctcgc cgcgcccggc atcgtaggtg atgatgagcg
     3241 tatcgcgatc gaacatgacc ccctggaagc gcgcgtaggg gcacatgtac ttgcacacct
     3301 gctcgcgcat ccagccggca ttaccgtatg tcgcgaagcc gtagaacaga atccagaacg
     3361 tctcccaggg cccgagggtc gccgtggcga tctcgcccat gagaacgtgg atcggcgtga
     3421 agtatccgac gaaggtaatg cccgtccaca gcgcaaaggc gatccacagt gcatgcttcg
     3481 ccgatttgag cgtgagcttg cgcgcgccgg cgggcgagcg gtcgagacgc atgcgttgca
     3541 cacgatcgcc ttcgacgaca cgctcgatcc acatgaagat ttcggtgtac accgtctgag
     3601 ggcaggcata cccgcaccac agccggcccg ccaccgcggt gaagagaaag agcgcgagcg
     3661 cggcaatcac cagcagcgcg gtgaggaaga tgaagtcctg cggccagaac acgtagccaa
     3721 agatgtagaa cttgcgggcg ccgagatcga acagtaccgc ctggcggccg ttccacgtga
     3781 gccagggcaa cccgtagaac acgagctgcg tcgcgaccac cagcagcaca cgccaggtgg
     3841 cgaaccagcc gtgaaccgcg cgcggataga tcttctggcg gatctcgtag agccccgcgt
     3901 cgacattctc gccgctgcgc gcgccgttca cgactccg
//
LOCUS       DASURG010000301         4144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_461785, whole genome shotgun sequence.
ACCESSION   DASURG010000301 DASURG010000000
VERSION     DASURG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461785"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1881)
                     /locus_tag="VFY80_05240"
     CDS             complement(109..1881)
                     /locus_tag="VFY80_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6007343.1"
                     /translation="MKNPIPPVIALAAVLGACAQQPVAPTAGSAPDATAAQPKPKVAA
                     SPARSQPQLPAHELTEQLMLKLMLAEVAVQRGQPHVAVPAYLDLARETRDPRIAQRAT
                     EVAWNARFVDSAIEAAGIWLKADSQSQQARQVLAALLVNQAQLTTALPHLETWLRSDP
                     ANVGQSFLQITSLLARHKDKNAVLELMQGLARSYSEVPEARFAVAQAAWNAQNADLAL
                     SEVREALRLRPDWELAALFQAQVLQRQSGPEALAYLSTYLDQHPRAKDARLSYARMLV
                     SEKQFPEAKKQFEVLLAEHPDNPEVTMAVALLALQLKDYDAAETHLKRALEIKYREPD
                     TVRMYLGQVNEERKRYDEALKWYGAISSGEQYISAQLRYAGVLSKQGRLGDARKHLQA
                     ITPRNAQQQVQLTQAEAQLLREASAYQDAYDLLTRALDKFPDYPDLLYDHAMAAEKVD
                     RIDVLETNLRKLISLRPDHAHAHNALGYTFADRNERLDEARELIETALKLAPEDPFIM
                     DSMGWVLYRQGQLPQALDYLKRAHALRPDPEIAAHLGEVLWVSGQRDEARAVWDEVLK
                     SHPTNEVLQNTVNRFLRTNRPAAR"
     gene            2033..2980
                     /locus_tag="VFY80_05245"
     CDS             2033..2980
                     /locus_tag="VFY80_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6007344.1"
                     /translation="MTEHDTATTLCARGLSRRFGMHAAVNDVSFELKRGEVLGFLGPN
                     GAGKTTTMHMLTGNLAPTLGSITVCGIDLLEQPTRAKAKIGYLPEIPPLYRELTVDEY
                     LRLAARLHRVERSACADAVKRARQRCGLADAGRKLIGTLSKGYQQRVGIAQAIVHDPE
                     VLILDEPTAGLDPNQIREIRTLIRELGGKHSVILSTHIMSEVEAVCDRVQIMHHGRLV
                     YSDTIGALEKFHSGRTLLVGLRQAPAPSALAAIAGITGVERIGDTVFRIQCSDGADPT
                     DELVRHAVAGGWGLYQLQPAQTTLEDVFVQLTRSEALAA"
     gene            2977..3747
                     /locus_tag="VFY80_05250"
     CDS             2977..3747
                     /locus_tag="VFY80_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6007345.1"
                     /translation="MIFTIAAKELKLLFASPLAWVVLTLTQIVFGYTFLRRIDDFLQV
                     QPRLTQMTSAPGVTELVIAPTFGTAAVVLLFAVPILAMRLIAEERRNHTMVLLNSAPV
                     TVTAIVLGKFLGLMGFLLLLIALLLLMPFALAGSTVLDYGLIASLVAGVVLLAASFAA
                     VSLYLSSLTTHPVVAAIGAFGVLLAMLLAGETAGESLRARGWQVPAALMQVFTPVRNF
                     EPLGEGLVDTYALACSLLLIAAAIVLAMRRLDALRLRG"
     gene            3753..>4144
                     /locus_tag="VFY80_05255"
     CDS             3753..>4144
                     /locus_tag="VFY80_05255"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX6007346.1"
                     /translation="MEASRRLRQRLYVQNGIFIVLLVALVMLLAFLAREYRKEWDVTR
                     TGRNSLSASTLELLGQLEGPLKITAYATAFDASGANVHKRIEERLRPYRRAKPDIALA
                     FVDPREQPRLAEAAGLRTPNELVIEFRRR"
BASE COUNT          670 a         1349 c         1341 g          784 t
ORIGIN      
        1 gcactcgacc cgacagatcg aacggcaggg gcgcgcgcgg cggcaggccg agcgtcgcgc
       61 acgccgccaa ggcgagcgcg gcggcgagcg cagcgatacg cgaccgggtc atcgcgcggc
      121 ggggcggttt gtcctgagga agcggttgac cgtattctgc aacacttcgt tcgtcgggtg
      181 gctcttgagg acttcatccc acaccgcgcg cgcttcgtcg cgctgcccgc tgacccacag
      241 gacctcgccg aggtgcgcgg cgatttcggg atcgggacgc agggcgtgag cgcgcttgag
      301 gtaatccaga gcctgaggaa gctgcccttg ccggtagagc acccaaccca tgctgtccat
      361 gatgaacggg tcctcgggcg cgagcttgag cgcggtttcg atgagctcgc gcgcttcgtc
      421 caggcgttcg ttgcgatcgg cgaaggtgta cccgagcgca ttgtgtgcat gcgcgtgatc
      481 gggccgcagc gaaatcagct tccttaggtt tgtttccagc acgtcgatgc ggtccacttt
      541 ctcggccgcc atcgcgtggt cgtagaggag atccggatag tcgggaaact tgtctagtgc
      601 ccgggtgaga agatcgtacg cgtcctgata agccgaagcc tcgcgcagaa gctgcgcctc
      661 cgcctgcgtg agctgcacct gctgctgcgc attgcgcggc gtgatggcct gcagatgctt
      721 gcgggcatcg cccagccggc cttgcttcga cagcacgccg gcatagcgca actgcgcgct
      781 tatgtactgc tcgcccgagc tgatcgcacc gtaccatttc agcgcctcgt cgtaacgctt
      841 gcgctcctcg ttcacttgcc cgaggtacat gcgcacggta tccggctcgc gatacttgat
      901 ctcgagcgcg cgcttgaggt gcgtctccgc ggcgtcgtag tccttgagct gcagcgcgag
      961 cagcgcaacc gccatcgtca cctcgggatt gtccggatgc tcggcgagca gcacttcaaa
     1021 ttgctttttc gcttccggaa actgtttctc tgataccagc atgcgggcat acgagaggcg
     1081 cgcgtctttc gcccggggat gctgatcgag ataggtggag aggtaagcga gcgcctcggg
     1141 tccggactgg cgctgcaaga cctgcgcctg gaacagcgcc gcgagctccc agtcgggacg
     1201 caggcgtagc gcttcgcgca cctcgctcag cgcgaggtcg gcgttttgcg cattccacgc
     1261 ggcttgggcc accgcgaacc gcgcttccgg tacttcgctg taggagcgtg ccagcccttg
     1321 catgagctcg agaacggcgt tcttgtcctt gtgccgcgcg agcagcgacg tgatctgcag
     1381 gaagctctgg cccacattcg cagggtcgga gcgcagccac gtctcgagat ggggcagcgc
     1441 ggtcgtgagt tgcgcctggt tgacgagcag cgccgccagt acctgacgcg cctgctgcga
     1501 ctgcgaatcc gctttgagcc agatgcctgc cgcttcgatg gcggaatcga caaagcgcgc
     1561 gttccatgcg acctccgtcg cgcgctgtgc gatgcgcgga tcgcgcgtct cgcgcgcaag
     1621 atcgagatac gcgggaaccg ccacgtgcgg ctgcccacgc tggactgcaa cttccgccag
     1681 catgagcttc agcatgagtt gctcggtcag ctcgtgcgcg ggaagctgcg gttgcgagcg
     1741 cgcggggctt gccgcaacct tcggcttcgg ttgcgcggcc gttgcgtccg gcgcgctccc
     1801 tgcggtaggg gctaccggct gctgcgcgca cgcaccgagc actgcggcga gcgcgatcac
     1861 gggcggtatg gggttcttca taatgtggcg gccgatcgcg accgcgcggg acagtgccgt
     1921 gaaattagga gtgcccaaag gaaaaaagcc gggcgcggct tcataaagca ccacatatta
     1981 ggtatatttt gccctcttca caagacgccg tcgccctgct ccacgcgatt cgatgaccga
     2041 acacgacacc gccacgacgc tctgcgcgcg cggtctttcg cgtcgcttcg gaatgcatgc
     2101 cgcggtgaac gacgtgagtt tcgaattgaa gcgtggcgaa gtgctcgggt tcctggggcc
     2161 caatggtgca ggcaagacaa ccaccatgca catgctgacc gggaacctcg cgccgacgct
     2221 cggcagcatc acggtgtgcg gcatcgatct actcgaacag ccgacgcgcg cgaaggcgaa
     2281 gatcggctac ctgcccgaaa tccccccgct ttaccgggaa ctcaccgtag acgagtatct
     2341 caggctcgcg gcgcgactgc atcgcgtcga gcgctccgcg tgtgcggatg cggtgaagcg
     2401 cgcgcggcaa cgatgcgggc tcgccgacgc tggcagaaaa ctgatcggca cgctgtcgaa
     2461 aggctaccaa cagcgtgtcg gcatcgcgca ggcgatcgtc cacgatcccg aagtgctcat
     2521 actcgatgag ccgaccgcag gtctcgatcc caatcagata cgcgagatcc gtaccctgat
     2581 tcgcgaactg gggggaaaac acagtgtgat tctttccacg cacatcatgt cggaagtcga
     2641 agcggtatgc gaccgggtgc agatcatgca tcatgggcgg ctcgtttata gcgataccat
     2701 aggcgcgctc gaaaagtttc atagcggtcg caccctgctc gtcggtctgc ggcaagcgcc
     2761 ggcgccgagc gcgcttgcag ccatcgcggg cattacgggt gtcgagcgca tcggagatac
     2821 ggtatttcgc atccagtgca gcgacggcgc cgatccgacc gacgaactgg tgcggcatgc
     2881 ggtcgcgggc ggctggggac tctaccagct gcaaccggcc cagacgacgc tggaggacgt
     2941 cttcgtgcaa ttgacccgca gcgaagcgct cgccgcatga tattcacgat cgccgccaag
     3001 gagctcaagc tgctcttcgc ttcaccgctc gcctgggtgg tgctgaccct gacgcagatc
     3061 gtgttcggtt acacctttct ccgccgcatc gacgatttcc tccaggtcca gccgcgcctc
     3121 acccagatga cgagtgcgcc cggcgtcacg gagctcgtca tcgcgccgac tttcggcact
     3181 gccgctgtgg tgctcctctt cgcggtgccg atactggcca tgcggctcat cgcggaggag
     3241 cggcgcaatc acaccatggt gctgctcaat tcggcgccgg tcacggtaac cgcgatcgta
     3301 ctcggcaagt ttctgggact catgggattt ctcctgctgc tcattgccct tctgctgctg
     3361 atgccgtttg cgctcgccgg cagcaccgtg ctcgactacg gactgatcgc gagcctcgtc
     3421 gcaggcgtcg ttctcctcgc cgcctcgttc gcggcggtga gcctttatct ttcatccctc
     3481 accacgcacc cggtggtagc cgccatcggt gcattcggcg ttctgctcgc catgctgctc
     3541 gctggtgaga ccgcaggtga gagcctgcgg gcgcgcggct ggcaggtgcc ggccgcgctg
     3601 atgcaggtgt tcacgccggt gcgcaatttc gagccgctgg gggaggggct cgtcgatacc
     3661 tatgcgcttg cctgctccct gcttctgatc gccgccgcga tcgtgctcgc gatgcgccgg
     3721 ctcgatgcct tgcgcctgcg cggctgagcg ccgtggaagc ctcgcgtcgc ctgcgccagc
     3781 ggctgtacgt gcagaacggc atcttcatcg ttttgctcgt ggcgctcgtc atgctcctcg
     3841 ctttccttgc acgcgaatac cgcaaggagt gggacgtgac ccgcaccggc cgcaacagcc
     3901 tgtccgcttc gacgctggag cttttgggcc agctcgaagg cccgctcaag attacggcct
     3961 acgcgaccgc gttcgatgcg tcgggcgcca acgtccacaa gcgcatcgag gaacgcctgc
     4021 ggccctatcg gcgcgccaag cccgacatcg cgctggcgtt cgtcgacccg cgcgagcagc
     4081 cgcgccttgc cgaggcggca ggcttgcgca cgccgaatga gttggtcatc gaattccgcc
     4141 gccg
//
LOCUS       DASURG010000302         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_53361, whole genome shotgun sequence.
ACCESSION   DASURG010000302 DASURG010000000
VERSION     DASURG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53361"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFY80_05260"
     CDS             <1..419
                     /locus_tag="VFY80_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX6007347.1"
                     /translation="PGRPTLILDVAHNPAAAEALAVTLSRMGRGGRTLVVFAMLRDKD
                     IDGVIAAFGKRIAIDHWFVAGIDAPRGADAALLAEKLQSAQVPGAVTVCESIAQAYAQ
                     ACDMAVQNDKILAFGSFYTVAAVIEARTKQRASSVP"
     gene            462..1130
                     /locus_tag="VFY80_05265"
     CDS             462..1130
                     /locus_tag="VFY80_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6007348.1"
                     /translation="MEMAKPISDEEVQLRKRARRRLIGAIAIVTAVVVALPMVLDNEP
                     KPLTQEIDIRIPSQDAAGPFISKVVPIPDPSQAPSKPAAEPKSEVKPEGTSAAQSSVA
                     PKPQPAAEEKPAPNADKAPTAPPARKAASAPKTEPQPSKAPAAPGQYVVQVVALSDAG
                     KAKQMQQAILKTGVKAYTEVVKTARGDVTRVRAGPYASREAAEKAQQRLKGAGFDGKV
                     VPAR"
     gene            1127..1618
                     /locus_tag="VFY80_05270"
     CDS             1127..1618
                     /locus_tag="VFY80_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX6007349.1"
                     /translation="MTAFDYAVLVIVGISILISVIRGLVREVLALLAWLAAFVAATFL
                     GAGVAAWMPHAIPTEELRLLTGFLVVFVGVLIAMSLIAMLVSRLIRRAGLGVEDRMFG
                     MLFGVARGAVVVLLLVLLAGLTPLPRDPMWRNAMLSSPLESMALYVKAWLPGELSRRI
                     KYD"
     gene            1629..>2822
                     /gene="purF"
                     /locus_tag="VFY80_05275"
     CDS             1629..>2822
                     /gene="purF"
                     /locus_tag="VFY80_05275"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX6007350.1"
                     /translation="MCGITGVVAKGPVNQILYDGLLVLQHRGQDAAGIVTADGASFHM
                     HKGGGMVRDVFRTRNMRELVGTAGIAHTRYPTAGSAWSAAESQPLYVNSPFGIVLGHN
                     GNLTNSEQLKSELFRQDLRHVNTNSDSEVLLNVLAHELHENATNYKLDPATIFAAVSG
                     VHRRCRGAYAVVAMIAGYGLLAFRDPYGIRPLVFGRIETDDGDEYLIASESVALDTLG
                     FELVRDVAPGEAIFIDFDGNFYSRQCATNPSLNPCIFEFVYLARPDSVIDGISVYETR
                     LRMGESLADKIRRQYAHLDIDVVIPIPDSSRPAAMELSKRLNLPYREGFIKNRYIGRT
                     FIMPGQSIRKKSVRQKLNAIAMEFKGKNVLLVDDSIVRGNTSSEIVQMARDSGARKVF
                     FASAAP"
BASE COUNT          511 a          877 c          932 g          502 t
ORIGIN      
        1 tgcccggacg accgacgctg attctcgacg ttgcgcacaa tcccgctgcc gccgaggcgc
       61 ttgccgtcac gttgagccgg atggggcgcg gcggacgcac gctggtagtt ttcgcgatgc
      121 tgcgcgacaa agacatcgac ggtgtgatcg cagcattcgg aaagcgcatc gccatcgatc
      181 actggttcgt cgccggtatc gacgcgccgc gcggcgccga tgccgcatta ctcgcggaga
      241 agctgcagag cgcgcaggtg ccaggcgcgg tgacggtgtg cgaaagcatt gcacaagcct
      301 acgcacaagc ctgtgatatg gcggtgcaaa atgataaaat tctcgcgttc ggctcgttct
      361 atacggtcgc tgcggtgatc gaggcgcgga cgaagcagcg cgcatcctcc gttccgtagg
      421 gatggccggc atttcagcat acgagcgcca actatggctt gatggagatg gcaaaaccga
      481 tcagcgacga ggaagtgcag cttcgaaagc gcgcccgccg gcggctgatc ggcgccatcg
      541 cgatagtgac agcggtggtc gtggcgcttc ccatggtgct cgacaacgag cccaagccgc
      601 tcacccagga aatcgacatc cggattccct cccaggatgc cgccgggcct ttcatttcga
      661 aggtcgttcc gatacccgat ccgtcacagg cgccgtccaa gccggcagcc gagccgaaat
      721 ccgaagtgaa gcccgagggc acgagcgctg cacaatcgag cgtggcgccc aagccccagc
      781 ccgcggcaga agagaaaccc gcacccaacg ctgacaaggc gcccactgca ccgcctgccc
      841 ggaaggcggc atctgctccc aagacggagc cgcagccgag caaggctccc gcggcgccgg
      901 gccagtatgt cgtgcaggtc gtcgcactct cggacgccgg gaaggcgaag cagatgcagc
      961 aggcgatcct caagaccggc gtcaaggctt ataccgaagt ggtgaaaacc gctcgcggcg
     1021 acgtgacgcg agtgcgggcg gggccgtatg cttcgcgcga agcggccgag aaggcgcagc
     1081 agaggctgaa gggcgctgga ttcgacggaa aggtcgtgcc tgcgcgatga cagccttcga
     1141 ctacgctgtt ctggtgatcg tcggcatctc gatcctgatc agcgtaatcc ggggcctggt
     1201 gcgggaagtg ctggcgctgc tggcatggct cgccgccttc gtggcggcaa ctttcctggg
     1261 tgcgggcgtc gcggcttgga tgccgcacgc gattccgacc gaggaactgc ggctgctgac
     1321 gggctttctc gtcgtattcg ttggcgtgct gatcgccatg agcctcatcg cgatgctggt
     1381 atcgaggctc atcaggcgcg ccggcctggg cgtcgaggac aggatgttcg gcatgttgtt
     1441 cggcgttgca cgcggggcgg tcgtcgtgct gcttctggtg ctgcttgccg gtctcacgcc
     1501 gctgccgcgc gacccgatgt ggcgcaacgc gatgttgagc tcgccgctcg agtcgatggc
     1561 gctctacgtg aaggcctggc tgccgggcga actgtcgcgg cggatcaaat acgactgagg
     1621 acagcgcgat gtgcggaata accggtgtgg tggcaaaggg tccggtcaac cagatcctct
     1681 acgacgggct gctcgtcctc cagcatcgcg ggcaggacgc tgccggcatc gtcaccgctg
     1741 acggcgccag cttccatatg cacaagggtg gcggcatggt gcgcgacgtc ttccgcacgc
     1801 gcaacatgcg cgagctcgtc gggaccgccg gcatcgcgca tacgcgttat cccaccgcag
     1861 gctccgcctg gtccgctgcc gagtcccagc cgctctacgt caattcgccg ttcggcatcg
     1921 tgctcggtca caacgggaat ctcaccaatt cggaacagct gaagagtgag ctcttccggc
     1981 aggatttgcg tcacgtcaac accaattccg attccgaagt gctgttgaat gtgctcgcgc
     2041 acgagctgca cgagaacgcc accaactaca agctcgatcc ggcgaccatc ttcgcggcgg
     2101 tctcgggcgt gcaccgccgc tgtcgcggtg cgtatgcggt ggttgcgatg attgcgggct
     2161 acggtctgct cgcgttccgg gatccgtacg gcatccggcc gctcgtattc ggacgtatag
     2221 agacggacga cggcgacgag tatctgatcg catccgaaag cgtcgcgctt gacacgctgg
     2281 gattcgaact ggtgcgcgac gtggcgccgg gcgaggcgat cttcatcgac ttcgacggca
     2341 atttctacag ccgccagtgt gcgaccaatc cgtcgctcaa tccgtgtatc ttcgagttcg
     2401 tctatcttgc gcgccccgac tcggtgatcg acgggatctc ggtgtacgaa acgcggctcc
     2461 ggatgggcga gagcctcgcc gacaagatcc ggcgccagta tgcgcatctc gacatcgacg
     2521 tggtgatccc gatcccggat tcgagccggc ctgcggcgat ggagctctcg aagcggctca
     2581 atctgccgta tcgcgagggg ttcatcaaga accgctacat cggccgcacc ttcatcatgc
     2641 cgggacagtc gatccggaag aaatcggtgc gccagaagct gaacgcgatc gcgatggagt
     2701 tcaagggcaa gaacgtgctg ctcgtggacg attcgatcgt gcgcggcaat accagcagcg
     2761 agatcgtaca gatggcgcgc gactcggggg cgcgcaaggt gttcttcgcc tccgcggcgc
     2821 cg
//
LOCUS       DASURG010000303         6377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_53373, whole genome shotgun sequence.
ACCESSION   DASURG010000303 DASURG010000000
VERSION     DASURG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53373"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1244
                     /locus_tag="VFY80_05280"
     CDS             261..1244
                     /locus_tag="VFY80_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX6007351.1"
                     /translation="MEIEIGSPMLYAAFSAAVVVLLVADFVLLRAQGNHQVPMREAAA
                     WSAAWFALAMAFAGWIWWHVGESHGEAVAKAKAVEYVTGYLIEKALAMENVFVWVTIF
                     TFFATPAELQKRVLLYGVVGAILMRAALIYLGVVLIQKFDWIFYVFGAFLIVTGVKML
                     FFTSGPADLSANPLLVWMRRHLRVTERYHGDRFVVVRNGLRYATPLFLVLVLVEVSDL
                     IFAVDSIPAIFAVTSDPFIVFTSNIFAILGLRAMYFLLADMAGRFHLLNYGLAFIVSF
                     VGLKMLIMDWYKIPITAMLAVVFTALIVSVAASLVIAPRRVRETPLRDRAG"
     gene            1465..1692
                     /locus_tag="VFY80_05285"
     CDS             1465..1692
                     /locus_tag="VFY80_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007352.1"
                     /translation="MAEISARNARGELHKLLLEQREVDELLTNLAPASSIETRLEIAA
                     ASLHRLDDSQLLALICEVLHGREKQAGVLKP"
     gene            1770..2090
                     /locus_tag="VFY80_05290"
     CDS             1770..2090
                     /locus_tag="VFY80_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007353.1"
                     /translation="MSERYEVISPEGLRRVPNMSLSHPIGSLEGKTIGEVYNNHFQGE
                     LMFHTYRRLFQERFPGVKVVPFTEFPRVFVGGDPDSQKRTAREIAGLAKARGCDALIS
                     GNGG"
     gene            2112..3461
                     /locus_tag="VFY80_05295"
     CDS             2112..3461
                     /locus_tag="VFY80_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007354.1"
                     /translation="MRPAVEAEKAGMPAVVVTTTGFTTIARAAARAEGMSELRIAEYP
                     GAVGVHSEELIVRNVIDVLFDRIVESLTRPVDGRSKEPAGSARRADDIISEGTYDEIN
                     DDFRIREWTDGLPVVPPTLDRVTAFLEHTDRAPDETIAVLPQANLRAVPWNIAANAVM
                     AGCRPQSMPLLIAAVEAIADNAYNLNNIGSTWGVLPFLLVNGPAIEKLGIESGAQLIS
                     RGANPAIGRALGLIIRNIAGYKLGRNYMGTFGYPLCFTLAENEKESPWPPYHVERGCS
                     SDESAVTVGATVTWGWPPAIYGTADKAAAQTALEFLSVEITKKPCLARLAERGPQGFR
                     NMITILLAPPIAKSLAAAGYTKEKIREYLHEHARVPYRELEFLLKYGHSEAFTIPDAV
                     RAGVYPEEYLVGPDELVRVVPSPDTIHIVVCGDPHRNRAMVLWGGYVNPVTRKIAVR"
     gene            3509..4825
                     /locus_tag="VFY80_05300"
     CDS             3509..4825
                     /locus_tag="VFY80_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6007355.1"
                     /translation="MDFAQDATFERLDARTVHEIKRHLIDTLASALGAYEEPVSVMSR
                     ALAERYHGDGAARVWASNIFASPEFAAFANGVMVRALDVSDTYLGRSRGHPSDMISGL
                     IAIGELERAGGRRLIEAIAVAYDVYCSFCEHIDLNAKGWDQPVYGVLGCVLGAARLMG
                     LSAEQTAHALSLAITPNMALAQARRGELSRWKGCAGANASRNAVFAAMLAKEGFTGPA
                     APFEGSGGLWEAIGRPDWPLPDVPLIARTHTKSLPVCYHGQSAVLAALELRARVDPHA
                     IEHIRIETYEAAVFMMGRDPSRWAPATRETADHSLPYCVAIALLDGEVTQASFADSRL
                     RDAAVKEIMRKTSVAEDRALSALYPESAPGRITVTMRSREVHVAEVRYPRGHEKNPMT
                     DAEIEQKFVALSAGRLSPERGKKVIAMLWQLELMQDVRTLTALISQ"
     gene            4838..5806
                     /locus_tag="VFY80_05305"
     CDS             4838..5806
                     /locus_tag="VFY80_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007356.1"
                     /translation="MGNALRLLVIFATCWGFAACVHAQRYPERPIRLIVPFAPGGTSD
                     LVGRVLGAKLSDELGQPVIVDNRGGAGSTLGTGIAARATPDGYTLIVSHIGLAINQTL
                     YSQIPYNAMKDLAPISKLGVAPSAVVVNNKLAAANLKELVALAKKQPGKLNYGSGGVG
                     SAGHLSVALLEHVAGVKFTHVPYKGGGPSVAATIAGEVELAIPVAASAAPHVRAGRLR
                     LLAVTSGKRSQAFPDVPTAQEAGVPGYVYETWFGLFAPAGTPKGIIARLHQATVKALD
                     MKEVRDQLFSQGVEADSSTPAELARILRDDTATWAKIIRSAGIPIN"
     gene            6131..>6377
                     /locus_tag="VFY80_05310"
     CDS             6131..>6377
                     /locus_tag="VFY80_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007357.1"
                     /translation="MRRIADGEAAAQGPLSAKELQRIDAYWRAANYLSVGQIYLLDNP
                     LLREPLSLRHVKPRLLGHWGTTPGLNFVYVHMNRAIRQ"
BASE COUNT         1160 a         1982 c         2055 g         1180 t
ORIGIN      
        1 cgactccgtg gtcctgccgc agtaaaagct tggctggccg ctgcaacggg gagggcggcc
       61 gcgtgccgcc cttcctgttt ctattgaccc gtggcgcggc cccgctcgat cgggcgcgct
      121 cctgctgcca catttgcgaa ctctgcgtac tgactgaaca ctttctatgc tgcgcgggcg
      181 agctccggtt caggagtcag acccgccgga agtttgaggt ttctaccgtg aaccgtttca
      241 ctgtacaaca ctgagccgcc atggagatcg agatcggcag tccaatgctg tacgccgcat
      301 tctccgcggc cgtcgttgtg cttctcgtcg cggacttcgt cctgctccgc gcacagggca
      361 atcaccaggt ccccatgcgg gaggccgcag cctggtcggc ggcttggttc gcgcttgcca
      421 tggcgtttgc cggctggatc tggtggcacg tgggtgaatc tcatggcgag gctgtcgcca
      481 aagctaaggc cgtagagtac gtcaccggct atctcattga gaaagccctg gcaatggaga
      541 atgtgttcgt ctgggtgacg atcttcactt tcttcgccac gccggccgaa ttgcagaagc
      601 gcgtgctcct ctacggcgtc gtgggcgcca tcctcatgcg tgccgcgctc atatatctcg
      661 gcgtggtgct gatccagaag ttcgactgga tcttctacgt gttcggcgct ttcctcatcg
      721 tcacgggcgt gaagatgctc ttcttcacga gcgggccggc cgacctgtcg gccaacccgc
      781 tgctcgtctg gatgcgccgg cacctgcgcg tcacggaacg ttaccatggc gaccgtttcg
      841 tcgtcgtgcg caatgggctc cgatacgcca ccccgctctt tctggtgctg gtgctggtcg
      901 aggtgagcga cctcatcttt gcggtggata gcattccggc catcttcgcg gtgacgagcg
      961 acccgttcat cgtcttcacc tcgaacatct tcgccatcct cggcttgcgc gcgatgtact
     1021 tcctgcttgc ggacatggcc gggcgcttcc atctgctcaa ctacgggctt gccttcatcg
     1081 tcagcttcgt gggcctcaag atgctcatca tggattggta caagatcccg attacggcga
     1141 tgctggcggt ggtgttcacc gcgctcatcg tctcggtcgc cgcgagcctt gtcatcgcac
     1201 cgaggagggt gcgagaaacg ccgctacgcg atcgcgcggg ttgaggccca ccgactgaca
     1261 tctggcgtcg gcttggcgcg ctggacggag aacgatacga ttcccgacgt ggtctagctt
     1321 ttcatcgcca taccgaatga ggtcacaatg gcatccatga tgagccgtct gaatggaaag
     1381 acgcgccgca gcgcccacca gacgattgct gcatcgattc acggagggcc agagcgaaag
     1441 acgcgcgggg aagaagatcg cttcgtggcc gagatttcag ctcgcaacgc ccgtggagag
     1501 cttcataagc tccttctcga gcaacgggag gtcgacgagc tgctgaccaa tcttgcgccg
     1561 gcaagcagca tcgaaacgcg actcgaaatc gcggctgcct cgctgcacag gctcgatgat
     1621 tcgcagcttc ttgcgctcat ctgcgaagtg ctccacggca gggaaaagca ggccggtgtg
     1681 ctgaaaccgt aggccgactg gcgcgcgacc ttctttcgct ggtagattac cgcggcgaag
     1741 tccggcccct gtgcgccgag gagtccacat tgagcgaaag atacgaagtg atcagcccgg
     1801 aagggctgcg gcgcgttccg aacatgagct tgagccaccc tatcggctcg ctcgagggaa
     1861 agacgatagg cgaggtctac aacaatcact ttcagggcga gctcatgttc catacctatc
     1921 gccgcctttt tcaggagcga tttcccggcg tcaaagttgt cccgttcacc gagtttccga
     1981 gagtcttcgt cggcggcgat cccgactcgc agaagagaac tgcacgcgaa atcgcgggac
     2041 tggcgaaggc gcgcggctgt gatgcgctca tcagcggcaa cggcgggtga ggtacgtgca
     2101 cgccggcctc ggtgaggccg gccgtcgaag cggagaaggc cggcatgccg gccgtcgtgg
     2161 tcacgaccac cggattcacc acgatcgcgc gcgccgctgc acgagccgaa ggcatgtccg
     2221 agctgagaat cgcggaatat cccggcgcgg tcggcgtcca ttccgaggaa ctgatcgtac
     2281 gaaatgtcat cgatgtgctc ttcgaccgca tcgtcgagtc gctgacccgc ccggttgacg
     2341 gcagaagcaa agagcccgct ggcagcgcgc gccgcgccga tgacatcatc agcgaaggga
     2401 cctacgatga gatcaacgat gacttccgca tccgcgaatg gacggatggg cttcctgtcg
     2461 ttccgccaac gctggatcgg gtcacggctt ttctcgagca tactgatcgc gcgcccgacg
     2521 aaacgatcgc ggtgctgccc caggcaaacc tgcgggcagt accatggaac attgccgcaa
     2581 acgccgtcat ggccgggtgt cgtccgcagt ccatgccgct cctcatcgcg gcggtagagg
     2641 cgatcgcgga caacgcgtac aacctgaaca acatcgggag cacctggggc gtgctaccgt
     2701 tcctgctcgt caatggtccg gcgatcgaaa agctagggat cgagagcggc gcgcagttga
     2761 tttcccgcgg cgcgaatccg gcgatcgggc gggcgcttgg cctcatcatc cgcaacatcg
     2821 cgggttacaa gcttggccgc aattacatgg gcaccttcgg ctatccgctc tgcttcacac
     2881 tggcggaaaa cgagaaggaa agtccgtggc cgccgtatca cgtcgagcgc ggctgctcct
     2941 cggacgagag cgcggttacg gtgggagcga cggtcacatg gggctggccg cctgcgatct
     3001 acggcaccgc ggacaaggcg gcggcgcaga ccgcgctcga gttcttaagc gtggaaatta
     3061 cgaagaagcc ctgtctcgcc cggcttgcag aacgcgggcc gcagggtttc cgcaacatga
     3121 tcacgatcct gcttgcgccg ccgattgcca agtcgctcgc cgcagcgggt tatacgaaag
     3181 aaaagatacg cgagtacctc cacgagcacg cgcgcgtgcc gtaccgcgaa ctcgaattcc
     3241 tgctcaagta cggacactcc gaggcattca cgattcccga tgcggttcga gcgggcgtct
     3301 acccggaaga gtacctcgtg gggcccgacg agctcgtgcg cgtggtgccc agccccgata
     3361 ccatccacat cgtcgtctgc ggcgatccgc atcgcaatcg cgccatggtg ctgtggggcg
     3421 gatacgtgaa tccagtcacg cggaagatcg ccgtccgcta atgggcgtcg ctctgcaaga
     3481 gcattgggac gacacgacgc gccgcctcgt ggacttcgcg caagacgcga ccttcgagag
     3541 gctcgatgcc cgtacggtgc acgagatcaa gcgccatctc atcgatacgc tcgcaagcgc
     3601 gctcggggct tatgaggagc cggtgtcagt catgtcccgc gctctcgccg agcgctatca
     3661 cggggatggc gcagcgcgcg tctgggcaag caacatcttt gcttcgcccg aattcgcggc
     3721 gttcgcgaac ggcgtgatgg tgagagcgct ggacgtgagc gacacctacc tcggccgcag
     3781 tcgggggcat cccagcgaca tgatctccgg gctcatcgcg atcggcgagc tcgagcgcgc
     3841 gggcggcagg cgcctgatcg aagcgattgc cgtcgcctac gatgtctact gcagcttttg
     3901 cgagcacatc gatctcaacg ccaaaggctg ggaccaaccg gtttacggcg tgctgggatg
     3961 tgtgctcggt gctgcgcgac tcatggggtt gtccgccgag caaactgcgc acgcgctttc
     4021 gctcgcgatt acgccgaaca tggcgctcgc tcaggcgcgc cgcggtgagc tttccaggtg
     4081 gaaaggctgc gccggcgcga atgcgtcgcg caatgcggtg tttgcggcga tgctggccaa
     4141 agagggcttt acggggccgg ctgcgccctt cgaaggctcg ggtggcctgt gggaggcgat
     4201 tggacgcccc gactggccgc tgccggatgt gccgcttatc gcgcgcaccc ataccaagag
     4261 tctgcccgtt tgctatcacg gccagtccgc ggtgctggcg gcgctcgagc tgcgcgcccg
     4321 cgttgatccg catgcaatag agcacatcag aatcgagacg tacgaagcgg cggtgttcat
     4381 gatggggcgc gatccttcgc gatgggcgcc ggcgacgcgc gagacggcgg atcacagcct
     4441 gccctattgc gtggcgatcg cgcttctgga cggtgaagtg acccaggcgt cattcgccga
     4501 ttcccgtctt cgcgatgctg cggtgaagga gatcatgcgc aaaacaagcg tggccgaaga
     4561 cagggcgctt tcggcgctct atccggagag cgcgcctggg cgtatcacgg ttaccatgcg
     4621 ctcgagggag gtgcacgtcg cggaagtgcg ctaccccagg ggccacgaga aaaatccgat
     4681 gacggatgcg gagatcgagc agaagttcgt cgcgctcagt gcagggcggc tcagccccga
     4741 gcgcggcaag aaggtgatcg ccatgctgtg gcagctcgag ctcatgcagg atgtgcgcac
     4801 gctgaccgcg ctcatctcac aatagaggag gaagacgatg ggtaatgcgc ttcgactgct
     4861 ggttatcttt gcaacgtgct ggggattcgc agcgtgcgtg cacgcgcaac gctatcccga
     4921 acgcccgatt cggctgatcg ttccgttcgc gccagggggg acgagcgatc tcgtcggccg
     4981 tgtgttgggg gcgaagctaa gcgatgagct gggccagccg gtgatcgtgg acaaccgcgg
     5041 cggcgcggga agcacgctcg gcacgggcat cgcggcgcgg gcgacgccgg acggctacac
     5101 gctcatcgtt tctcacatcg gccttgcgat caaccagacg ctctactcgc aaataccgta
     5161 caacgcgatg aaggatctcg cgccgatctc gaagcttggc gtcgcgccgt ctgcggtggt
     5221 ggtgaacaac aaactcgccg cggcgaatct gaaggagctc gtggcactgg cgaagaagca
     5281 accgggaaag ctcaactacg gatcgggcgg cgtcggtagt gcgggccatc tttcggttgc
     5341 gctcctcgag catgtcgcgg gtgtcaagtt cacgcacgtg ccgtacaagg gtggtggccc
     5401 gtcggtcgct gccaccatcg cgggggaggt cgagctcgcg atcccggttg ccgcgagcgc
     5461 agcgccccac gtacgggcgg gccggctgcg tctgctcgcc gtgacgagcg gcaagcggtc
     5521 ccaggcgttt cccgatgtgc cgaccgcaca ggaggccggg gtaccagggt atgtctacga
     5581 aacttggttc gggctgttcg cgccggccgg gacgccgaaa ggaatcatcg cgcgactgca
     5641 tcaggcgacg gtcaaggcgc tcgacatgaa agaggtgcgc gatcagctct tcagtcaggg
     5701 cgtagaggcg gattcttcca cgcccgcgga attggcgagg attctgcgcg atgacacggc
     5761 gacctgggcc aagatcatcc gcagcgccgg cataccgatc aattaagcgc ggtccggcgg
     5821 gtgcgatgct atacttagcg cacacggaaa gaatcgcgac ttgaccaaag acccttccaa
     5881 aatcaagcgg tgccccgaat gccggcggct tgtggaggcc tccacgatgc gtccgctgtc
     5941 ccgcacccgg tttggcggcg gcgtgcgcta cgcctgccct gactgcttca agcgcgtcat
     6001 ggccttgcgc aaggccgtcg caagcgcccg cggcacaaaa tagatccggg cgaattccgc
     6061 acgtaacgtc cggaacgggc gcttccccgt cttgactaag cggtattgac cgcaacaggg
     6121 ggacgcttcc atgagacgca tcgccgacgg cgaagccgcc gcgcaaggcc cgctttccgc
     6181 taaggaactg cagcgcatcg acgcctactg gcgcgcagcc aattatctct ctgtcgggca
     6241 gatctacctc ctcgacaatc cactgctgcg agaaccgctc agtcttaggc acgtcaagcc
     6301 gcgccttttg ggccactggg gcaccacacc gggcctcaat ttcgtctacg tccatatgaa
     6361 tcgtgccatc cggcagc
//
LOCUS       DASURG010000304         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_155544, whole genome shotgun sequence.
ACCESSION   DASURG010000304 DASURG010000000
VERSION     DASURG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155544"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFY80_05315"
     CDS             complement(<1..915)
                     /locus_tag="VFY80_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX6007358.1"
                     /translation="MPQLRIEDYALIGDCETAALVARNGSIDWLCWPRFDSPACFASL
                     LGTSEHGHWQIAPADPNARARRRYQPDTLVLETEFETASGGFTLVDFMPIGEEGSDLV
                     RTLVGRSGAVPVRMELVLRFDYGASVPWVSRLPDGRLRAIAGPDMVLLSSPVQIRGEN
                     LTTVAEFEVREGDRLSFVMMHTASHVQPPPPLDPEAALEKTLHHWREWAGHCSAGGEY
                     AEPVRRSLLTLKALTYSPTAGIVAAPTASLPEQLGGKRNWDYRYCWLRDATLTLLALM
                     AGGYGGEARAWREWLVRAVAGSPSQMQIM"
     gene            complement(1226..2068)
                     /locus_tag="VFY80_05320"
     CDS             complement(1226..2068)
                     /locus_tag="VFY80_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6007359.1"
                     /translation="MSAVRSVALRGGETVPALGQGTWYMGEQAAKRKQEIAALRLGIE
                     LGMTLIDTAEMYGNGRAEALVAEAIRGMRERVFIVSKVLPSNASRKGVRRACEASLQR
                     LRTDRIDLYLLHWASSHPLEETVRGFEDLIAAGHIRYWGVSNFDCREMENTFGIAGGT
                     ACAANQVLYNLTRRGIEHDLMEWCRRRGVAIMAYSPIEQGRLLDRRELKHLAQRHGAT
                     PSQVALRWVLRQDGIIAIPKAGTPEHVRENRAALDLALSLEDLSALDRAFPPPGGPMP
                     LEML"
     gene            complement(2065..>2445)
                     /locus_tag="VFY80_05325"
     CDS             complement(2065..>2445)
                     /locus_tag="VFY80_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEX6007360.1"
                     /translation="AAWEACRRDPRSPAVVMPQYLAWLKALPGKPVFVAYPAAFDFMF
                     VHWYLNRFAGESPFAHSALDIKTYAMALLGTAYYDSTKRNMPKEWFDPLPHTHIALDD
                     AKEQGVLFCNILAWRDRTLGPGVP"
BASE COUNT          415 a          806 c          797 g          427 t
ORIGIN      
        1 catgatctgc atttgcgacg ggctgccggc gacggcgcgc acgagccatt cacgccacgc
       61 tcgcgcttcc ccgccgtaac cgccggccat cagcgcgagc agcgttagcg ttgcatcgcg
      121 cagccagcaa tagcggtaat cccaattgcg cttgccgccg agctgctcgg gcagcgaagc
      181 ggtcggtgcg gcgacgattc cggccgtcgg cgagtacgtc agcgccttga gcgtgaggag
      241 cgagcggcgc accggctcgg cgtactcgcc accggcgctg cagtggcccg cccattcgcg
      301 ccaatggtgc agcgtttttt ccagcgcggc ctcgggatcg agtggcggcg gcggttgcac
      361 gtgagaggca gtgtgcatca tgacgaaact gagccggtcc ccttcacgca cctcgaattc
      421 ggcgaccgtg gtcaggttct cgccccggat ttgcacgggc gaagacagaa gcaccatatc
      481 gggccccgca atcgcgcgca ggcggccgtc gggcaggcgg ctgacccacg gtaccgatgc
      541 accgtaatcg aagcgcagta cgagttccat gcgcaccggc acggctccgc tgcgaccgac
      601 cagcgtgcgc acgaggtccg acccctcctc cccgatcggc atgaaatcga cgagggtgaa
      661 cccgccgctc gcggtttcga actcagtctc caggaccagc gtgtcgggct ggtaacggcg
      721 gcgagcccgg gcgttcgggt cggcaggcgc gatctgccaa tggccgtgct cgctcgtacc
      781 gaggagcgat gcaaagcatg ccggagaatc gaagcgcggc cagcacagcc agtcgatgga
      841 gccgttgcgc gcaacgagcg cggccgtctc gcagtcgccg atgagggcat aatcctctat
      901 gcgcaattgc ggcagccgga tctcacctcc ggaatggcgt tggatcatcg ctctgcggtc
      961 atgacgatcg cttgaccgct ttcttttctg gcggtttcgg ccccggttcc cgcacgggcg
     1021 gcggctcgcg ccccggtcgc ttgacgggtg gacgcttttg ccctttcgcc tttgtcgcca
     1081 tggtgcagtc gctccgcaat tcgatccgtc gtgccattcg tgcctcaatc ttcggaccgc
     1141 tgagcgcgca aaagttcgga gggtgcgttt acgggatata ccgaccctgg tgcaggacga
     1201 tggcgggatg agatcagcgg ccggtctaca gcatctcgag cggcatcggc ccgcccggcg
     1261 gcggaaacgc gcgatcgagt gcggagagat cctcgaggct gagcgcaaga tcgagcgcag
     1321 cacgattctc gcgaacgtgc tccggggtgc cggcctttgg gatggcaatg atgccgtcct
     1381 ggcgcagaac ccatcgcaat gcgacctggg acggcgtcgc gccatggcgt tgcgcgaggt
     1441 gcttcaattc ccggcgatcg agcagccggc cctgctcgat tggcgagtat gccatgatcg
     1501 cgacgccccg cctgcggcac cattccatga gatcgtgctc gatgccgcgg cgggtgaggt
     1561 tgtagagcac ctgattcgcc gcgcacgccg tcccgcccgc aatgccgaaa gtgttttcca
     1621 tttcgcggca atcgaaattg ctgacgcccc agtaacggat gtgaccagcc gcgatgagat
     1681 cctcgaaccc gcgcaccgtt tcctccagcg ggtggctcga ggcccagtgc agcaaataga
     1741 gatcgatgcg atcggtacgg agccgctgca ggctggcctc gcacgcgcgg cgcacgcctt
     1801 tgcggctcgc gttcgacggc agcactttgc tcacgatgaa cacccgctcg cgcatgccgc
     1861 gaatcgcttc tgcgaccagc gcttcggcgc ggccgttgcc gtacatctcg gcggtatcga
     1921 tgagcgtcat gccgagctcg atgcccagtc gcaaagcagc gatctcctgc ttgcgcttcg
     1981 ccgcctgctc gcccatgtac caggtgccct gaccgagcgc cggtaccgtc tccccgccac
     2041 gcagcgcgac tgaacgcacg gcgctcatgg cacgccgggg cctaacgtcc gatcgcgcca
     2101 cgcaaggatg ttgcagaaga gtacgccctg ctccttggcg tcatcgagcg cgatatgggt
     2161 gtggggcagc gggtcgaacc attccttcgg catgttgcgt ttcgtcgagt cgtaatacgc
     2221 cgtgccgagg agcgccatgg cataggtctt gatatcgagc gccgagtgcg cgaatgggct
     2281 ttcgccggca aagcggttca agtaccagtg cacgaacatg aaatcgaatg ccgcgggata
     2341 ggcgacgaac acgggcttgc cgggcagcgc cttcaaccac gcgagatact gcggcatgac
     2401 cactgcaggg ctacgcggat cacgccggca cgcttcccat gccgc
//
LOCUS       DASURG010000305         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1485168, whole genome shotgun sequence.
ACCESSION   DASURG010000305 DASURG010000000
VERSION     DASURG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485168"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1729)
                     /locus_tag="VFY80_05330"
     CDS             complement(242..1729)
                     /locus_tag="VFY80_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HEX6007361.1"
                     /translation="MRTRINASWIVGHANGQHQLIRDGVVVYDGNRIVHVGKRFEGHV
                     DKVIEARNKLAAPGFIDTHVHSGHRASHRLITDTGRPMYYGQPFLEISVPKEGKIVKG
                     DPRYLRHGDAGSEAAFQLNATFTVAELLRNGVTTFVEYGSQLRVQDALLAEVKRLGTR
                     AYLAPGYDCGRWVGDERGRLKRVRDDAMGLKGLETALDWIARHDGDADGRVRGILVPR
                     EVETSSIELLERTRAAADERKLPMATHAAYSVLEFHDIVREHMKTPIELLEELGMLRP
                     TINIGHGNFISDNPNLNYSGGHDLSLMGNACATVSHCPINIVRRARTLDNWKKYEDAG
                     VNMTIGSDTYPRDMVMNMRTASYMGKLMSHSYFAASAGDVVRAATLGGAISVGRDDLG
                     RLAPGALADIILIDLTGRGTLRYGPVRDPVKSVVECGVGDDVDTVIVDGKICMEGGVI
                     PGVDFAKLREEAQAAGEQVWETLSEWDPLGRSADEASPWSYPVVS"
     gene            complement(1749..>3003)
                     /locus_tag="VFY80_05335"
     CDS             complement(1749..>3003)
                     /locus_tag="VFY80_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX6007362.1"
                     /translation="GGAAKASVVSSGLFGTVSGSAVANVLVDGIITIPLMKRTGFKGT
                     FAGAIEATASTGGQLAPPVMGAAAFILADIVGVSYATVAFAAIVPAVLYYLSLYIVVD
                     SYSRRMGLGPNPALPVAESFAGLRERWHILIPLGVMIYLLTNQYSLMMVGAVSLIVIV
                     ILSWARVSTRLNLRAIYEAIVNGARGAAEVAIPSAVAGIIVGVLIQTGMALHLERWLL
                     NLAGNSLLVSLIGAMFLTLIFGMGMPTSAAYLVAAVLVGPALQSLGIPALAAHLFIFY
                     FAVLSMVTPPVALAAYAAAGISGANLWTTGLVAFMVAIPGFLIPFAFVFDQGILLQGT
                     LLHIAHVVATAAIGVTGLSVATGGYLYGPLTRSARVALFCCAPLLIDPDPVTDVIGGA
                     GIALVLGYQVWRYKLSRPKASGAPL"
BASE COUNT          554 a          973 c          959 g          517 t
ORIGIN      
        1 tgccggacag ggtcacctgt tgcgcggccg cgatacgaga gggatcggcc agctcggaaa
       61 gcgtgggcag cgtgacgcgc agctcgcgca accgccgcac ggcgctttcg cgcgctttcc
      121 tgtcggcgat ctcgggcgtg aggctcaatt cactcattga acgcttgctt gttttgcgga
      181 gagaaactaa cggcggtgaa gtcgtctcta tgacgccgac cgcgtgtgcg catgatacta
      241 actatgatac caccggatag ctccacggcg acgcctcgtc cgcgctgcgg ccgagcggat
      301 cccattcgga gagtgtctcc cacacctgtt cgccggccgc ctgcgcctcc tcacgcagct
      361 tcgcgaaatc cacaccgggg atgacgccgc cttccatgca gatttttcca tcgacgatta
      421 cggtgtccac gtcgtcgccg acgccgcatt ccaccacgct cttcacggga tcgcgcaccg
      481 gcccgtagcg cagcgtgccg cgtccggtca gatcgatcag gatgatgtcc gcgagcgcgc
      541 ccggcgcaag ccggccgaga tcgtcgcgcc cgaccgagat cgcgccgccg agcgtcgccg
      601 cgcggaccac gtctcccgcg ctcgcggcga aatacgaatg gctcatgagc ttgcccatgt
      661 atgacgcggt gcgcatgttc atcaccatgt cgcgcggata ggtatccgag ccgatcgtca
      721 tgttgacgcc tgcgtcctcg tatttcttcc agttgtccag cgtgcgcgcc cgccgcacga
      781 tgttgatcgg gcaatgggag accgttgcgc acgcgtttcc catcagactc aggtcgtgcc
      841 ccccggagta attgagattg ggattgtcgg agatgaagtt gccgtgcccg atgttgatgg
      901 tcgggcgcag catcccgagt tcttccagca gctcgatcgg cgtcttcatg tgctcgcgca
      961 cgatgtcgtg aaactcgagc acactgtagg cggcgtgggt cgccatcggt agcttgcgct
     1021 cgtcggctgc cgctcgcgtg cgctcgagca actcgatgct cgaggtctcc acttcgcggg
     1081 ggaccaggat gccgcgcact ctcccgtcag cgtcgccgtc gtgcctcgcg atccagtcga
     1141 gcgcggtttc aagccccttg aggcccatcg cgtcgtctcg gacgcgcttc aggcgtcccc
     1201 gctcgtctcc cacccagcgc ccgcagtcgt agcccggcgc aagataggcc cgcgtgccaa
     1261 ggcgtttcac ctccgcgagc agcgcatcct gcacgcggag ctgactgccg tactcgacga
     1321 aggtcgtgac gccgttgcgc aggagttccg caacggtgaa agtggcattg agctgaaagg
     1381 ccgcctccga gcctgcatcg ccgtgcctga ggtaacgcgg atcccctttg acgatcttgc
     1441 cctccttcgg cacgctgatc tcgagaaacg gctgcccgta atacatcggc cggccagtgt
     1501 cggtgataag gcggtgcgag gcgcgatgtc ccgaatgcac atgggtatct ataaagccgg
     1561 gggctgcgag cttgttccgc gcttcgatga ctttatcgac gtgcccctcg aagcgcttgc
     1621 ccacgtgcac gatccggttg ccgtcgtaca ccacgacgcc gtcgcggatg agttgatgct
     1681 ggccgtttgc atgacccacg atccagcttg cgttgattcg ggtgcgcata tcacgtgctc
     1741 cggagaaatt aaagcggtgc gcccgaggcc ttggggcgcg aaagcttgta acgccacacc
     1801 tggtagccca ggacgagggc gatgccggcc ccgccgatca cgtcggtgac agggtcaggg
     1861 tcaatcaaca gcggcgcgca gcagaacagc gccacgcgcg cagatcgggt gagcggtcca
     1921 tagagatagc ctccggtggc gacggagagc ccggtcacgc cgatcgccgc cgtagcaacg
     1981 acgtgtgcga tgtgcaacag agtcccctgg agaagtatcc cctgatcgaa cacgaatgca
     2041 aacggtatca ggaatccggg gatggcgacc atgaacgcga ccagccccgt ggtccacaga
     2101 ttcgcaccgc tgatgcccgc tgcggcgtac gccgccagcg cgaccggcgg cgtaaccatg
     2161 gagagcacgg cgaaataaaa gatgaagagg tgcgccgcca gcgcagggat accgaggctt
     2221 tgcagcgccg gccccacgag aactgcagcc accaggtagg ctgcggaggt gggcatgccc
     2281 atgccgaaga tgagcgtgag gaacatcgcg ccgatcagcg agaccagcag cgagttgccc
     2341 gccagattga gaagccagcg ctccaagtgc agcgccatcc cggtctggat cagcacgccg
     2401 acgataatgc cggcgactgc ggaagggatg gccacctcgg ctgcgccccg tgcgccgttg
     2461 acgatcgcct cgtagatcgc gcgcaggttg agccgggtgg aaacgcgggc ccagctcagg
     2521 atcacgatca cgatcaggct taccgcgccc accatcatca gcgagtactg gttggtcagc
     2581 agatagatca tcacgccgag cggaatgaga atatgccagc gctcgcgcag cccggcgaac
     2641 gattcggcta cgggtagggc gggattcggc cccaatccca tgcggcggct gtagctgtcc
     2701 accacgatat agagcgacag gtagtagaga acggccggca cgatggctgc aaatgcgacg
     2761 gttgcatagc tcacgccgac gatgtcggcc aggatgaatg cagccgcgcc catgaccggg
     2821 ggcgcaagct gcccgcccgt ggaagcggtc gcttcgattg cgcccgcgaa cgtgcccttg
     2881 aagccggtgc gtttcatgag gggaatggtg atgatgccat ccaccagcac gttggcgacg
     2941 gcgctgcccg atacggtccc gaagagcccg gacgagacaa ccgacgcttt cgccgcgccg
     3001 ccc
//
LOCUS       DASURG010000306         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_870193, whole genome shotgun sequence.
ACCESSION   DASURG010000306 DASURG010000000
VERSION     DASURG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870193"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1207
                     /locus_tag="VFY80_05340"
     CDS             29..1207
                     /locus_tag="VFY80_05340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6007363.1"
                     /translation="MDRLILRPGREKALKRRHPWIFSGAVERVEGHPGAGDTVEARSA
                     SGEFLAVAAYSPQSQIRARVWDWKSRIIDRQFFEEQVRRAISRRVCLAPEATDAARLL
                     NGEADGLPGVVADRYGDTLVLQVTSAGGERWREEIADILLAATGAVRVWERSDADVRV
                     LEGLSARSGVVRGTPPPSIVEIHEHGLKFEVELAQGHKTGFYLDQRDNRQRLRQLARG
                     CEVLDCFAYTGGFALNALAGGARSVAAIESSGTALERARANAALNGLPEAEWIEDDVF
                     QRLRRFRDEARRFDLIVLDPPKLAPTSAHADRAARAYKDANLLAFKLLREGGVLMTFS
                     CSGAVSADLFQKIVAGAALDAAVDAQIEAWLHAAPDHAVALNFPEGEYLKGLLCRVTR
                     "
     gene            complement(1197..1430)
                     /gene="yidD"
                     /locus_tag="VFY80_05345"
     CDS             complement(1197..1430)
                     /gene="yidD"
                     /locus_tag="VFY80_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX6007364.1"
                     /translation="MKSVLLALLDGYRLLLSPFFGSQCRFYPTCSQYAREAIELHGSV
                     RGSWLAVKRIARCHPWHPGGVDPVPPKRRRFTV"
BASE COUNT          375 a          735 c          723 g          356 t
ORIGIN      
        1 cgcgcgcaag cgcggctagc cgcaggcaat ggaccggttg atcctgaggc ccggacgcga
       61 gaaagcgttg aagcggcgcc atccttggat attctccggg gccgtcgagc gtgtcgaggg
      121 ccatcccgga gcgggcgaca ccgtggaggc gcgttctgcg agcggcgagt ttctcgccgt
      181 ggccgcgtac agtccgcagt cacagattcg cgcgcgcgtc tgggactgga aaagccgcat
      241 catcgaccgg caattcttcg aagaacaggt ccggcgggca ataagtcgac gcgtgtgcct
      301 cgcaccggaa gcgaccgacg ccgcccgact tctaaacggc gaagccgatg gcctgcctgg
      361 tgtcgtcgca gatcgctatg gcgacacgct cgtgctgcag gtgacgagcg ccggaggcga
      421 gcgctggcgc gaggagatcg cggacatcct gctcgcagcc accggggcgg tgcgggtctg
      481 ggaacgctcg gatgccgatg tgcgcgtcct cgaaggcctt tcggcgcgga gcggcgtcgt
      541 gcgcggcaca cctccccctt cgatcgttga gatccatgag catggcctca aattcgaagt
      601 ggagctcgca caggggcaca agaccgggtt ctacctcgac cagcgcgaca atcgacagcg
      661 cttgcggcaa cttgcgcgcg gttgcgaagt gctcgattgc ttcgcttata ccggcgggtt
      721 tgcgttgaat gcgctcgcgg gcggtgcacg atcggtcgcg gcgatcgaga gctccgggac
      781 ggcgctcgag agggcccgcg ccaatgccgc tctcaacggt ctgccggaag cggagtggat
      841 cgaggacgat gtctttcagc gtctgcgccg gtttcgcgat gaggcgcggc gcttcgatct
      901 catcgtgctc gaccccccca agcttgcgcc gacgagcgcg cacgcggacc gcgcggcgcg
      961 tgcctacaag gacgcgaacc tccttgcctt caagctgctg cgggagggcg gcgtgctcat
     1021 gaccttttcg tgctccggtg cagtgtcggc cgatctgttt cagaaaatcg tcgcgggtgc
     1081 ggcgctcgat gcagctgtcg acgcgcagat cgaagcctgg ctgcacgccg ctccggacca
     1141 cgctgtggca ctcaattttc cggagggtga gtacctcaag ggcctgctgt gccgggtcac
     1201 acggtaaatc gccggcgctt tgggggaacc gggtcgacac cgcccgggtg ccacggatgg
     1261 cacctcgcga tccgtttcac tgcaagccac gagccgcgca ccgagccgtg tagttcgatc
     1321 gcctcgcgcg cgtactgtga gcacgtcggg tagaagcggc actgcgatcc gaaaaacggg
     1381 ctgagcagca gccgatatcc atcgagcagc gcaagcagca cggatttcat ggtgcgtcga
     1441 gtatagcgcg cgaggcccag tgcgccgcgt cgcgcatggg ggactggggt agaatgcgcg
     1501 tccatcgcct tcgctgcgcg caattcggtt tggatcgtgg gcgtaccctc ccctgtcgcg
     1561 ccaccgcaaa tgccgttccg cctgaaaaac cgtcagcgcc gcgcgctcat cgtttttgga
     1621 acgggcagcg tgatcctgca tgccgtgctg ctcgatgccc tgcccgggtt cgaagcgccc
     1681 gatgtcccgg aggcacgggt gctcgacgtg gtccttgtat caccgccagc gccgcccatc
     1741 gctgccccac cgatcgtcac gccgcctgcg aaagcgcctc ccgagccacc gaagccggtc
     1801 gatcgcgcgg aaaagccaaa accgccggcg aaaaaaaagc gcccggaaga gcctcggccc
     1861 cgatccgggg aagcaccacg ggcactctcg agcgaacaaa tgacggccgt accttctccg
     1921 ccctcgttcg cagtaccacc ggcaccacct tcccgcgctc ccgatgtcgg cgcacgcagc
     1981 acagcgccgt cgcaagccaa ggcggacatc acgccccccg ttttcaacgc gtcctatctt
     2041 cgcaatccgg cgccacgata tcccttgatc gcacgccgca acggcgagca gggcaccgtc
     2101 acgctgcgcg tgctcgtcac gcgtgagggc acgccggcac gcgtatcggt cgagcagacg
     2161 agcggctcgc gccacctcga cgaagcggc
//
LOCUS       DASURG010000307         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_258993, whole genome shotgun sequence.
ACCESSION   DASURG010000307 DASURG010000000
VERSION     DASURG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258993"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1080
                     /locus_tag="VFY80_05350"
     CDS             112..1080
                     /locus_tag="VFY80_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007365.1"
                     /translation="MHRLIGVLLVAVIGTPVPDAHAQQYPTKPIRFIIPFPPGDSLDI
                     MSRLIAPHMLERLGRPIVVDNRPGAAGQLGLTLGARAPADGYTVIGGQGGNMVVQPHT
                     YRKLPYDPLKDFVPVAVSTTNYLALIASPNAPFKDVKQLVAYAKANKGKLIFASNGEG
                     GFPHMSMEMLRVAAGLFDYTHIPYKGTGDIMNDLVSSRVDTTILGIGSITPFVKAGRV
                     RMLAVTSPNRQDMWPGIPSIAEVLPGYDSRGWFGYVAPAGTPKEIVAILNREINRAMS
                     MPDVKEKLNAIGLTVVAESPEYFGQLLKSDYQKYGKLIRDIGFKPQ"
     gene            1169..>2027
                     /locus_tag="VFY80_05355"
     CDS             1169..>2027
                     /locus_tag="VFY80_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6007366.1"
                     /translation="MATARDPYMRSEIRDGMRITWHQPIEVDDGLVLRADVYRPVEET
                     PCPVILTYGIYGKGLAYQDGYPLQWEKMVADHPEILKMSSNKYQNWETTDPECWVPHG
                     YAVVRVDSRGAGWSPGFMDPASPREVEDLYQCIEWAGTQPWSNGKVGMLGISYYASNQ
                     WRVAGMHPPHLAAIIPWEGQNDRYRDSGYHGGILCEFQKRWAKHQVVNIQYGRGEHAK
                     KNPNTGESVAGPVTLSEEELARNRVDAFEELKKHPLADEWHRGRSADLSLVRTPLLTC
                     ANWGGQGIHP"
BASE COUNT          401 a          647 c          628 g          351 t
ORIGIN      
        1 ccgaaatcga tgatgtgtaa tgaacgcgag ctaacaccgg tacgccgccg ggcgccgcct
       61 ccgggtgtat ggcagaatga cgccgttcca gttccggagg gagctcgcat catgcatcgc
      121 ctgatcggtg tgttgctggt cgctgtcatc ggcacccccg ttccagacgc gcacgcccaa
      181 cagtacccca cgaagccgat tcgcttcatc attcccttcc cgccgggcga ttcgctcgac
      241 atcatgtcac ggctcatcgc gccacacatg ttggagcggc ttgggcgccc gatcgtggta
      301 gacaaccgac ccggggcggc cgggcaattg ggccttacgc tgggcgcgcg cgctcccgcg
      361 gacggctaca ccgttatcgg cggccagggc ggcaacatgg tcgtgcagcc gcacacctac
      421 cgcaagctgc cctacgatcc gctcaaagac ttcgtgccgg ttgcggtctc gaccacgaat
      481 tatctcgcgc tcatcgcgag cccgaatgcg ccgttcaagg acgtgaagca gctcgtcgca
      541 tacgcgaaag cgaacaaggg caagctcatt tttgcatcga acggcgaagg cggattcccc
      601 cacatgtcga tggaaatgct gcgcgtcgcg gccgggctct tcgactatac gcacattccg
      661 tacaaaggca ccggtgacat catgaacgat ctcgtctcga gtcgcgtgga cacgaccatc
      721 cttggcatcg gttccatcac gccgttcgtc aaggcgggac gggtgcgcat gctggcggtc
      781 accagcccga accggcaaga catgtggccc ggcatcccct ccatcgcgga agtgctgccc
      841 ggctacgact cgcgcgggtg gttcggttac gtagcacccg ccggcacgcc gaaagagatc
      901 gtggcgatcc tcaatcgcga gatcaatcgc gcgatgagca tgccggacgt gaaagagaag
      961 ctcaacgcga tcggtctcac cgtggtggcc gaatcgcccg agtatttcgg gcagttgctc
     1021 aagtcggact accagaagta cgggaagctc attcgcgaca tcggattcaa gccgcagtag
     1081 cgccttcttg cgcgtgccct aaatcctctc ccctgtccac tgggtaaaag cgagacgtta
     1141 ttccgttttt ttcgttcagg agtccagcat ggccacagca cgagacccgt atatgcgcag
     1201 cgaaatccgc gacggcatgc ggatcacctg gcaccagccg attgaggtgg acgacggcct
     1261 cgtgctgcgc gcggacgtgt atcgaccggt cgaggagacg ccctgccctg tcatcctcac
     1321 ctacggcatc tatggcaagg ggctcgccta ccaggacggc tatccgctgc agtgggaaaa
     1381 gatggtggcg gaccatccgg aaatcctgaa gatgtcgtcg aacaagtatc agaactggga
     1441 gacgacggac ccggaatgct gggtgccgca cggctacgcg gtggtgcgcg tggattcccg
     1501 cggggcggga tggtcgcccg gctttatgga ccccgcttcg ccgcgagaag tggaagatct
     1561 ctatcaatgc atcgagtggg ccggcacgca gccgtggagc aacggcaagg tcgggatgct
     1621 cggcatttcc tactacgcga gcaaccagtg gcgcgtggcg ggcatgcatc cgccgcacct
     1681 tgccgccatc atcccgtggg aaggccagaa cgaccgctac cgcgactcgg gctaccacgg
     1741 cggcatcctg tgcgagttcc agaagcgctg ggcgaagcac caggtcgtga acatccagta
     1801 cggccggggc gagcacgcga agaagaaccc gaacaccggt gagtcggtcg ccgggccggt
     1861 cacactctcg gaagaggagc tggcaagaaa tcgcgtcgac gctttcgagg aattgaagaa
     1921 gcatccgctc gccgacgaat ggcatcgcgg tcgctccgcc gatctctcgc tcgtgcgcac
     1981 gccgctcctc acctgcgcca actggggcgg gcaaggcatc catccgc
//
LOCUS       DASURG010000308         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_565209, whole genome shotgun sequence.
ACCESSION   DASURG010000308 DASURG010000000
VERSION     DASURG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565209"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..>2530
                     /gene="mutS"
                     /locus_tag="VFY80_05360"
     CDS             367..>2530
                     /gene="mutS"
                     /locus_tag="VFY80_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX6007367.1"
                     /translation="MMQQYFRIKAQYPDMLLFYRMGDFYEMFYEDAERAAQLLDITLT
                     TRGASAGEPIRMAGVPYHSVEQYLAKLVKLGESVAICVQIGDPNASKGPVERRVTRVV
                     TPGTLTDSALLEEKRDNVLLALHFDRTAAGLAWLSLSSGRFSVMETAANDLASELERL
                     RPSEILISEERDAPALPNGSTAVKRLPPWHFEFDAARRNLCRQFETRDLSGFGADDMR
                     VAIGAAGALLEYARSTQGTAIAHVKSLAVEQERAFVRMNPATRRNLELTETIRGEPAP
                     TLFSLLDTCASGMGSRLLRHTLHHPLRDRAALGERLAAVRALMGAAGDGPHQALHDDL
                     RRCVDVERITARIALKSARPRDLSGLRETLTRLPAISALLGLAPAPRLAELAVTLSPQ
                     SELHALLSAAIKPEPAAVLREGGVIADGFDPELDELRAIQTNCGQFLMELEARERARS
                     GIANLKVEYNRVHGFYIEVTRSQADNVPDDYRRRQTLKNAERFITPELKAFEDKALSA
                     QERALSREKMLFEQLLDDLAPHIPALQRAASALAELDMLATFAERAAALDWVMPEFAD
                     EPLIDIDAGRHPVVEAQVDHFIANDARLSDSRRLLIVTGPNMGGKSTYMRQVALIALL
                     ASCGSFVPATRARLGPVDQIFTRIGAADDLAGGRSTFMVEMTEAAYILHHATPESLVL
                     MDEIGRGTSTFDGLSLAWACARHLGRETGAFTLFATHYF"
BASE COUNT          438 a          839 c          811 g          442 t
ORIGIN      
        1 ctctttgggt tcgccgtccc tcgcaccttt gctgcgatga ttgtgcacac cgtcgttcag
       61 tagacgtcgc tcggctcccg agcgcgaaaa ccatccgcaa taaaccaaat ccgtctctgt
      121 aaggcggtgc gacaacgcag caccgtatcg cgaagtcgct catagatcgg gccacccgct
      181 aaaatggcgt cactggctcg cggtggatta agaaaaagcg atattttctg ctctgcattc
      241 cttttgttat gacatgctcg taaggcgcgc tcctgcgtca ggaccagcct tgcgggtaaa
      301 taacgtcaca tcgctgaagt gaaacgaaca gactcagagg gttccagccc ctcccaccat
      361 accccaatga tgcagcaata tttccggatc aaagcgcagt atccggacat gctgcttttc
      421 tatcgtatgg gggacttcta cgagatgttc tacgaggacg ccgagcgcgc ggcgcagctc
      481 ctcgacatca cgctcaccac gcgcggggcc tccgccggcg agccgatcag gatggcgggg
      541 gtgccctatc attccgtcga gcagtatctc gcgaagctcg tcaagctcgg cgaatccgtc
      601 gcgatctgtg tgcagatcgg agatccgaat gcgtcgaagg gccctgtcga gcgcagggtc
      661 acgcgtgtgg tgacgcccgg gaccctcacc gattccgcat tactcgagga aaaacgcgac
      721 aacgtgctgc tcgcgctgca cttcgaccgg acggcggccg ggctcgcgtg gctatcgctc
      781 tcttcggggc gcttcagcgt gatggagacg gcggcaaacg atctcgcgag cgagttggag
      841 cgattgaggc cgtcggaaat cctgatctca gaagagcgcg atgcacccgc gctgcctaat
      901 ggcagcacgg cggtaaagcg cctccccccg tggcatttcg aattcgacgc tgcgcgccgc
      961 aacctctgcc gtcagttcga gacccgggat ttgtcgggat tcggtgcgga cgatatgcgg
     1021 gtcgcgatcg gcgcggcggg cgcgctgctc gaatacgcac gctccacgca gggcacggcg
     1081 atcgcgcacg tgaagtcgct cgcggtcgaa caggagcgtg cgtttgtgcg catgaatcca
     1141 gcaacgcgcc gcaacctcga gttgaccgaa acgatccgcg gcgagcccgc gcccacgctc
     1201 ttttcgctgc tcgatacgtg cgcgagcggc atggggagcc ggctcctgcg ccacacgctg
     1261 catcatccgc tgcgcgatcg tgcagcgctg ggcgagcggc tcgcggcggt gcgcgcgctc
     1321 atgggcgccg cgggcgatgg cccgcaccag gcgctgcacg atgatctccg gcgctgcgtc
     1381 gatgtcgagc gtatcaccgc gcgcatcgcg ctcaagtcgg cgcgcccgcg cgatctctcg
     1441 gggctgcgcg agacgctgac gcggctgccg gccatttcgg cgctgctcgg gctcgcgcct
     1501 gcgccgcggc tcgccgagct tgcggtcacg ctttcgccgc aaagcgagct gcacgcgctg
     1561 ctctcagccg cgatcaagcc cgagcccgcc gccgtgctga gagagggcgg cgtgatcgcg
     1621 gacggcttcg acccggagct ggacgagctg cgcgccatac agacgaactg cggacagttc
     1681 ctgatggagc tcgaagcgcg cgagcgggcg cggagcggca ttgctaatct caaggtcgag
     1741 tacaaccgcg tgcatggctt ctacatcgaa gtgacgcgct cgcaggccga caacgtgccg
     1801 gacgactacc gccgccggca gacgctcaag aacgccgagc gctttatcac accggagctt
     1861 aaagcgttcg aggacaaggc gctctccgca caggagcggg cgctctcccg cgagaagatg
     1921 ctgttcgagc agctgctcga cgatctcgcg ccgcatattc ccgcgcttca acgtgcagcg
     1981 tcagcgctgg cggagctcga catgctcgca accttcgccg aacgcgccgc cgccctcgac
     2041 tgggtcatgc ccgaattcgc cgacgagccg ctgatcgaca tcgacgcggg gcggcatccc
     2101 gtggtcgagg cgcaggtcga tcatttcatc gccaacgacg cacggctttc cgactcgcgt
     2161 cgtctcctga tcgtcacggg gcccaacatg ggcggaaagt ccacttacat gcgacaggtc
     2221 gcattgatcg cgttgctcgc gagctgcggc tcgttcgtgc cggcgacgcg cgcgcgcctc
     2281 gggccggtgg accagatctt tacgcgcatc ggcgccgccg acgatcttgc gggcgggcgc
     2341 tcgaccttca tggtggagat gaccgaggcc gcctacatcc tgcaccacgc gacgccggag
     2401 agcctggtgc tgatggacga gatcggtcgc ggcacgagca cgttcgacgg cctctcgctc
     2461 gcgtgggcct gcgcgcgtca cctcggccgg gagaccggtg ccttcacgct gttcgcgacg
     2521 cactacttcg
//
LOCUS       DASURG010000309         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_361317, whole genome shotgun sequence.
ACCESSION   DASURG010000309 DASURG010000000
VERSION     DASURG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361317"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFY80_05365"
     CDS             complement(<1..647)
                     /locus_tag="VFY80_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007368.1"
                     /translation="MGIELESRRHTVSDADAAMELCYAKGWTDGLPVVPPTAERISAM
                     LDAGGLKPQQQLAFIENRQVSVTAEKAAINAVMAGCKPEYMSVVAAAVEALGDPLYGY
                     HGPATSTGGSAVFMVVNGPIARELDINCENNLFGPGWRANATIGRAVRLIMRNVIGTL
                     PGELDRSSLGHAGKYTFCIAENEAESPWPPFHTTRGFRADQNAVTIFAAYAPHQYS"
     gene            complement(750..1562)
                     /locus_tag="VFY80_05370"
     CDS             complement(750..1562)
                     /locus_tag="VFY80_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007369.1"
                     /translation="MAKLDGKVAFLTGAGAGIAKASALLFAREGARVAIAEINAEAGH
                     AAEAEVRKAGGDALFVETDVTQDESVKRAIDATVARFGRLDVLLNCAGGSLQEDVPVH
                     QMDLAVWQRTIALNLLHPFLVCRHGIPHMMGAGGGSIINVTSHLGLMGSVKPAYAATK
                     GGIISFTKTLAAQYVEHRIRANAIAPGSVRTERQIKRYESAEWTQSAKTGAAARERMI
                     AQKLYPYSVSEPADIAAIALFLASDESRTLTGTTIAADGGRSSYLKFYAGED"
     gene            1747..>2120
                     /locus_tag="VFY80_05375"
     CDS             1747..>2120
                     /locus_tag="VFY80_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007370.1"
                     /translation="MADGLKPIRRVVTGNDERGRSKVAWDGPAPNSHETSLGSGRGHT
                     DLWVWNETPAPLSGTNDDGNLKYTFCGPPNGGHFRVVQARPRPDDYDPAQDKDLVPPH
                     PPKFRAGGRGTWDRGGNNVYSSA"
BASE COUNT          358 a          712 c          686 g          364 t
ORIGIN      
        1 gagtactggt gcggcgcgta cgcggcgaag atggtgacgg cgttctgatc ggcgcgaaag
       61 ccccgcgtcg tatgaaacgg cggccacggg ctttcggcct cgttctccgc gatgcagaag
      121 gtgtacttgc cggcgtgccc gaggctgctg cggtcgagct ctccgggaag ggtgccgatg
      181 acgttgcgca tgatgagccg cacggcgcgc ccgatcgtcg cgttggcccg ccagcccggc
      241 ccgaagagat tgttctcgca gttgatgtcg agctcgcgcg cgatcggccc gttcaccacc
      301 atgaacaccg cggagccgcc ggtgctggtc gcggggccgt gatagccgta gagcggatcg
      361 ccgagcgctt cgaccgctgc ggcgaccacg ctcatgtatt ccggcttgca tcccgccatc
      421 acggcgttga tcgccgcctt ttccgccgta accgacacct ggcggttctc gatgaaagcg
      481 agttgctgct gcggcttgag tcccccggca tcgagcattg cgctgatgcg ctcggcggtc
      541 ggcggcacga ccggcaatcc gtcggtccag cctttcgcgt agcacagttc catcgcggca
      601 tccgcatcgg ataccgtgtg gcggcgcgac tcgagctcga ttcccacggg tggcctctct
      661 tgtgatgacg ttcgggaagc gttgcggcga gcccgcatta tgccctgcac cgcgccgcct
      721 gcaaaccgcg cggggcatta cgccggcctt cagtcctcgc cggcatagaa cttgagatac
      781 gacgagcgtc cgccgtccgc ggcgatggtg gtgccggtca gcgtgcgcga ttcgtcggac
      841 gcgagaaaga gcgcgatcgc cgcgatatct gccggctcgc tcacggaata gggatagagc
      901 ttctgcgcga tcatgcgctc gcgcgccgcg gcgccggtct tcgccgactg ggtccattcc
      961 gcgctttcgt agcgcttgat ctggcgctcg gtgcgcacag atcccggtgc aatcgcgttc
     1021 gcgcggatac ggtgctcgac gtactgcgcg gcaagcgtct tcgtgaacga gatgatgccg
     1081 cccttggtgg cggcgtatgc cggcttgacc gagcccatga gcccgaggtg cgaagtcacg
     1141 ttgatgatcg agccgcctcc tgcgcccatc atgtgtggga tgccgtgacg gcagacgagg
     1201 aacggatgca gcagattgag cgcgatcgtg cgctgccaga ccgccagatc catctggtgc
     1261 accggcacgt cctcctgcag cgagccgccc gcgcaattga gaagaacatc cagccgcccg
     1321 aagcgcgcga cggtcgcatc gatggcgcgc ttcacgctct cgtcctgcgt gacgtcggtt
     1381 tcgacgaaaa gcgcatcgcc gccggctttc cgcacttccg cctcggccgc atgcccggct
     1441 tcggcattga tttcagcgat ggccactctc gcgccttcgc gcgcgaacag cagtgcgctc
     1501 gccttggcga tcccggcgcc ggcgccggtg agaaatgcaa ccttaccgtc aagctttgcc
     1561 attacagatc ctttccaatg ggcgcattat agggcgcgcg cttcggtggc gtgctgcgaa
     1621 accaacgacg tcatgacgag acggccgcgc gaccttgtgt ggcataatcg aacggcattc
     1681 cgaggcagat cacctcacgg accgatcggc aacaattccg acacgttgac acaagagagg
     1741 agcgccatgg cagacggact caaaccgata cggcgcgtcg tcaccggcaa cgacgagcgc
     1801 ggccgctcga aagtggcatg ggacggcccc gcaccgaact cgcatgagac ctccctcggc
     1861 agcgggcgcg gccacaccga tctctgggta tggaatgaaa cccccgcgcc gctttccgga
     1921 acgaacgacg acggcaacct gaaatacacg ttctgcggcc cgcccaatgg cggccacttc
     1981 cgtgtcgtgc aggcgcgccc gcgcccggac gactacgacc ccgcgcagga caaggatctc
     2041 gtgccgccac acccgccgaa atttcgcgcc ggcggacgag ggacgtggga ccgcggcggc
     2101 aacaacgtct actcctcggc
//
LOCUS       DASURG010000310         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1485938, whole genome shotgun sequence.
ACCESSION   DASURG010000310 DASURG010000000
VERSION     DASURG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485938"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VFY80_05380"
     CDS             complement(<1..1453)
                     /locus_tag="VFY80_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6007371.1"
                     /translation="MSGAIVVIGSGVNELVAAHFLARAGHEVVVLEQHTPDDDFPSGW
                     IAPDIVRQLALSQVNAEHPLRIGQPDPWIAAALPDGGRLELWRDPVRSADAIRRLSTR
                     DAARWPEFCDRMARFARVLEKLYTAPPPDILSKDWRELARIAGLGIHVRRLGRRGMEA
                     FLRLLPMPVADLLDDWFESDALKGVLAGAAMMDLCQGPRSGGTAFRLLHHHVGNAPGV
                     FRPVRTNIRWILEKRPGISLRHRAGVTRIAVRERRAVAVVLESGEEIAARCIVSGVDP
                     RRTLLDWLDPGWLDPEFTRAVRHIRSRGVVARVVLTLDGEPGFNRLALAPSLDYVERA
                     YDDAKHARMSAHPYIEAHAADSPAHAVVAHVQYVPYALSNGAWDEAKRAALGERVVAI
                     LDRASPGFGARVTARRVLVPPDLEALYGYPQGQAHHAELALDQALWMRPLPDCAAYRT
                     PIEGLYLCGPATHPGGAIAGASGANAARIVLEDL"
     gene            complement(1450..>2257)
                     /locus_tag="VFY80_05385"
     CDS             complement(1450..>2257)
                     /locus_tag="VFY80_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6007372.1"
                     /translation="EIRTNASVSRLEIANGRVRTVVLESGEEIPATFVVSGADPRHTF
                     LSLVGRAHLPDDFAEGIDRYRFRGASGKVNLALSEPPNFTCLPGTGPHLRGAISISPS
                     VDYLERAYDEAKYGEPSSRPYLDIVIPSMIDPEMAPPGRHVMSIFVQYAPYRVNGGWT
                     DARREAFGDTVIDTLAEYAPNLKSAILHRQVITPADIERIVGLSEGNIFQGELTLQQM
                     FFLRPVPQWARYRTPIDGLYQCGSGTHPGGGVMGASGRLAAFAILRDRAR"
BASE COUNT          411 a          767 c          733 g          346 t
ORIGIN      
        1 gaagatcttc aaggacgatg cgggccgcgt tcgcgccgct cgcacccgcg atggcccctc
       61 ccgggtgagt ggccgggccg cagagataaa ggccctcgat tggcgtgcga tacgcggcgc
      121 agtccggcag cggccgcatc cacagcgcct gatcgagcgc gagctccgca tgatgcgcct
      181 gtccctgcgg atagccgtac agcgcctcga ggtcgggtgg cacgagaaca cgacgcgcag
      241 tgacgcgcgc gccgaagccc gggctcgccc ggtcgagaat agcgaccacg cgttcgccaa
      301 gcgcagcgcg cttcgcctcg tcccatgcgc cattcgacag ggcgtaaggc acatactgaa
      361 cgtgcgcgac gacagcgtgc gcgggcgaat cggcagcgtg agcctcgata tagggatgcg
      421 ccgacatacg ggcatgcttc gcgtcgtcat atgcgcgttc cacatagtcg agcgacggtg
      481 cgagtgcgag gcggttgaac cccggctcgc catcgagcgt cagcaccacc cgcgcgacaa
      541 ccccgcgcga acggatgtgg cggaccgcgc gtgtgaattc cgggtcgagc cagcccgggt
      601 caagccagtc gagcagggtc cgccgcggat ccactcccga tacgatgcag cgcgcagcga
      661 tttcctctcc gctctccagc acaaccgcta ccgcgcgcct ttcccgtacg gcaatgcgag
      721 tcacgccggc gcgatgccga agcgagatcc ccggccgctt ttccaggatc catcgaatgt
      781 tggtacggac gggacggaag acgcccggcg cattaccgac gtgatgatgc aggagcctga
      841 atgccgtgcc acccgaacgc ggaccctggc agagatccat catcgccgcg ccggcaagga
      901 cacctttcag tgcgtcgctc tcgaaccagt catcgagaag atcggcgacc ggcatcggca
      961 gcagtcgcag aaacgcttcc atgccgcgtc ggccgaggcg ccgcacgtga atgcccagcc
     1021 ccgcgatgcg cgcgagttcg cgccagtcct tgctgagaat atcgggcggc ggcgcggtat
     1081 agagcttctc cagcacgcgc gcaaagcgcg ccatccgatc gcagaactcg ggccaccgcg
     1141 cagcgtcccg cgtgctcaac ctgcgtatcg cgtccgccga tcgcaccgga tcacgccaca
     1201 attccagtcg accgccatcc ggcagcgccg ctgcgatcca tggatcgggc tgtccgatcc
     1261 ggagcggatg ctccgcattg acctgagaga gcgccaattg ccgcacgatg tccggcgcaa
     1321 tccagccgct cgggaagtca tcgtccggcg tgtgctgctc gagcaccacc acctcgtgcc
     1381 ccgcccgcgc cagaaaatgc gcagcgacga gctcgttgac gcccgatccg atgacgacga
     1441 tggcgccgct catcgcgcac ggtcccgcag tatggcgaac gcggccaggc ggccggaggc
     1501 gcccatgacg ccgccaccgg gatgcgtgcc ggagccgcac tggtagagtc cgtcgatcgg
     1561 cgtgcgatag cgcgcccact ggggcaccgg tcttaagaaa aacatctgct gcaaggtcag
     1621 ctcgccctgg aagatgttgc cttcgctcaa gccaacgatg cgctcgatgt ccgccggcgt
     1681 gatgacctgc cggtgcagta tggcggactt gagattcggc gcgtactcgg caagcgtgtc
     1741 gatcaccgta tcgccgaacg cttcgcgtct ggcatcggtc cagcccccgt tcacccgata
     1801 gggcgcgtac tgtacgaaga tcgacatcac gtgccggccc ggcggagcca tttcgggatc
     1861 gatcatcgag ggaatcacga tatcgagata gggccggctg gaaggctcgc catacttcgc
     1921 ttcatcgtag gcgcgctcga gataatccac gctcgggctg atcgagatcg cgccgcgcag
     1981 gtgggggccg gtgccgggca ggcacgtgaa gttgggcggc tcgctcagcg cgaggttgac
     2041 cttgccggac gccccacgga agcgataacg gtcgattccc tccgcgaagt cgtcgggcaa
     2101 gtgtgcgcgc ccgacgagcg agaggaacgt gtgccgggga tcggcccccg acacgacgaa
     2161 ggtcgccgga atttcctctc cgctttcgag cacgacggtc cgcacgcggc cattcgcaat
     2221 ctcgaggcgt gaaaccgatg cgttggtccg aatctca
//
LOCUS       DASURG010000311         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1075662, whole genome shotgun sequence.
ACCESSION   DASURG010000311 DASURG010000000
VERSION     DASURG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075662"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /gene="aspS"
                     /locus_tag="VFY80_05390"
     CDS             complement(<1..1467)
                     /gene="aspS"
                     /locus_tag="VFY80_05390"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX6007373.1"
                     /translation="MRSEYCGLVSREHLDSTVTLFGWVHRRRDHGGVIFIDLRDREGL
                     VQIVCDPDRPATFQTADRLRNEYVVKVTGRVRPRPEGTVNPNLVSGEVEVLAHDIEIL
                     NASLTPPFQMDDEQLSETVRLEYRFLDLRRPQMQQNLRLRYRTAMAVRQFLDRHGFID
                     IETPMLTRSTPEGARDYLVPSRVHAGHFFALPQSPQLFKQLLMISGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDIETSFLDQSEITDLMEALIRDVFKSVMDVELPQPFPRMRYDE
                     AMALYGSDKPDLRVPLKLTELSDLMREVEFKVFRDAAQLKDGRVAALRVPRGGALSRK
                     EIDDYTDFVKIYGAKGLAYIKVNDIAQGAAGLQSPILKFLPEATVRAILERSGAQNGD
                     LVFFGADRAKVVNEALGALRAKIGHEHGHLEAGWRPLWVLDFPMFEWDEDEKRWMAMH
                     HPFTAPADGHEDLLESNPAAARAKAHDMVLNGWEIGGGS"
     gene            complement(1520..>2049)
                     /locus_tag="VFY80_05395"
     CDS             complement(1520..>2049)
                     /locus_tag="VFY80_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX6007374.1"
                     /translation="GVHIPGLGVLLTLLVVFLTGVFAANMIGQRLVAFWEGLLLRIPV
                     VNSIYNGVKQVSDTLFSPTGQAFRKALLVQWPREGMWTIAFLTGTPGGDVANHLPGDY
                     LSIYVPTTPNPTGGYFVIVRRSDVIELDMTVDAALKYIVSMGVVAPSGMNRRIDARVL
                     APSGDAPRQPHAVNQ"
BASE COUNT          362 a          664 c          661 g          362 t
ORIGIN      
        1 cgaaccgcca ccgatctccc atccgttcag caccatatcg tgcgcttttg cgcgggcggc
       61 ggcggggttc gactcgagca ggtcctcgtg gccgtcggcg ggcgcggtga agggatggtg
      121 catcgccatc cagcgtttct cgtcctcgtc ccactcgaac atcgggaagt cgagcaccca
      181 gagcgggcgc cagccggctt ccaaatgacc gtgctcgtga ccgattttcg cgcgtagcgc
      241 gccgagcgct tcgttgacca ccttcgcgcg gtccgcgccg aagaagacga ggtcgccgtt
      301 ctgcgcgccg ctgcgctcga gtatcgcccg gaccgtcgct tcgggcagga acttgagtat
      361 cggcgactgc aggcccgcag cgccctgcgc gatgtcgttc accttgatat aggcgagccc
      421 cttggcgccg tagatcttga cgaaatcggt gtagtcgtcg atttccttgc gcgaaagcgc
      481 cccgccgcgc ggcacccgca gcgccgccac gcgaccgtcc ttcaactgcg ccgcgtcgcg
      541 aaagaccttg aattccacct cgcgcatgag gtcgctcaat tcggtcagct tgagtggcac
      601 gcggagatcc ggcttgtcgg agccgtagag cgccatcgct tcgtcgtagc gcatgcgcgg
      661 gaagggctgc ggcagctcga cgtccatcac actcttgaac acgtcgcgga tcagcgcttc
      721 catgagatca gtgatctcgc tctggtcgag aaaggaggtc tcgatgtcga tctgagtgaa
      781 ctcgggctgg cggtcggccc ggaggtcctc gtcgcggaag catttcacga tctggtagta
      841 gcggtcgaag cccgagatca tgaggagctg cttgaagagc tggggtgact gcggcagcgc
      901 gaaaaaatgc ccggcgtgca cgcgcgaggg cacgaggtag tcgcgcgcgc cttccggtgt
      961 cgagcgcgtc agcatcggcg tctcgatgtc gatgaaaccg tgccggtcga ggaactggcg
     1021 caccgccatc gcggtgcgat agcgcagccg caggttctgt tgcatctgcg ggcggcgcag
     1081 atcgagaaag cgatactcga gccgcacggt ctccgaaagc tgctcgtcgt ccatctggaa
     1141 cggcggcgtg agcgaggcat tcaggatctc gatgtcgtgc gccagcactt ccacttcgcc
     1201 gcttacgagg ttcggattga ccgtgccttc tgggcgcggc cgcacccggc cggtcacctt
     1261 gaccacgtac tcgttgcgca gccgatcggc ggtctggaaa gttgcgggcc ggtcgggatc
     1321 acacacgatc tgcaccagcc cttcgcggtc cctcaagtcg atgaagatga cgccgccgtg
     1381 gtcgcggcgc cggtgcaccc agccgaagag cgtgacggtg ctgtcgagat gctcgcggct
     1441 cacgaggccg cagtattcgc tacgcatgat tcaccacaag gatcgacgat cgcgaaaccg
     1501 ggatcgacag gcgcgatccc tactgattga cggcgtgcgg ttgccggggc gcgtccccgc
     1561 tcggcgcgag cacgcgcgca tcgatgcgcc ggttcattcc gctcggggcc acgacgccca
     1621 ttgagacgat gtacttcagt gccgcatcga ccgtcatgtc gagctcgatg acgtcgctcc
     1681 gccgcacgat cacgaagtag ccgccggtcg ggttgggcgt cgtcggcacg taaatgctga
     1741 gatagtcgcc gggcagatga ttcgccacgt caccccctgg ggtgccagtc aaaaacgcga
     1801 tcgtccacat cccctcgcgc ggccactgca cgagcaacgc cttgcgaaag gcctggcccg
     1861 tcggcgagaa cagcgtatcg ctcacttgct ttacgccgtt gtagatcgag ttgaccaccg
     1921 ggatgcgcag cagcaaccct tcccagaaag cgaccagccg ctggccaatc atgttggcgg
     1981 cgaagacgcc ggtcaggaaa acgaccagca gcgtcagcaa taccccgagc ccggggatat
     2041 ggaccccca
//
LOCUS       DASURG010000312         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_973882, whole genome shotgun sequence.
ACCESSION   DASURG010000312 DASURG010000000
VERSION     DASURG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973882"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..564
                     /locus_tag="VFY80_05400"
     CDS             223..564
                     /locus_tag="VFY80_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007375.1"
                     /translation="MHDILRDRLWRKLEALPEQQLYQVLDYIEFLESKYAPGQVRDPD
                     VLTRFAERFEDSMRMRSVAPKVIFGAVGLMGTARKVVRGVTDAGKDLLGALDTEAQDS
                     RKPGPPGGESK"
     gene            565..957
                     /locus_tag="VFY80_05405"
     CDS             565..957
                     /locus_tag="VFY80_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX6007376.1"
                     /translation="MAKLRIGRSQRRRQSAVKLLRELPNLIKLLVRLLRDARVSRLDR
                     VLFGAVIAYVLLPADLLPDFLGFLGLTDDLYLIGLALNRLFARAGADVLLEHWDGNPR
                     ALGYLIEGVEEVGGLLPRRIRNALRGLI"
     gene            974..2446
                     /gene="guaB"
                     /locus_tag="VFY80_05410"
     CDS             974..2446
                     /gene="guaB"
                     /locus_tag="VFY80_05410"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX6007377.1"
                     /translation="METPRNSRILGEALTFDDVLLVPDYSEVHPSATDVRSRLTNSIE
                     LSIPLLSAAMDTVTESRMAIAMAREGGIGIIHKNMPIERHASEVDRVKRSESGMIRDP
                     ITLRPDETLQDALRVMERFSISGVPIVENGGRLVGIITNRDLQFETALNRPIREVMTR
                     EGLITAPIGTSLEDAERILQRHRIEKLPVVDAQGNLRGLITVKDIFKRRQFPNACKDE
                     HGRLRVGAALGTAEKELERAAELLAAGCDVLVIDTAHGHSAGVLDAVARTRNRFPDAQ
                     IIAGNVATADAAKALVERGADAVKVGIGPGSICTTRVVAGIGVPQLTAIMESARGVDG
                     RVPVIADGGIRYSGDIVKAIAAGAASVMMGSLLAGTEESPGESFLLEGRRFKTVRGMG
                     SLSAMAEGSADRYFQESVAGSRKLVPEGIEGRVPYKGPASDVVFQLVGGLRSGMGYCG
                     TANIEALRTRAKFVRITSGGLRESHPHDVAITREAPNYHL"
     gene            2659..>3105
                     /locus_tag="VFY80_05415"
     CDS             2659..>3105
                     /locus_tag="VFY80_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007378.1"
                     /translation="MASGSTTDAGTVQVLRTELEALEGIERVVVEDSAIWFNCDPEAE
                     VSDLRTRAEEVLKRNGVDAEKLQLLFLTDLSARRIKFSRIERIVERTGNVRMRVALEW
                     QGLVKLGEASGESGDLLEQRTAATAALIALENVVGEPLGIKLVGVKH"
BASE COUNT          599 a          955 c          991 g          560 t
ORIGIN      
        1 taaggatttc gcggcatagc tcgttacgat ccgtgccgtc atcactcaat gcttcgtcga
       61 ggtggcccag ccagcgctca tacgcctgcg tcgcgtgagg cgcgatcgca acctgtctca
      121 gtggtagaaa agccatgttg ggcgcaaagg taggccgctg cctctatacg agcaacggct
      181 gttggcctat gattcgcacc ataaaatgtc aggggaactg ctatgcacga cattctgagg
      241 gaccgcctct ggcgcaagct cgaggcgctg cccgaacaac agctctacca ggtgctggac
      301 tacatcgaat ttctggaatc gaaatacgca ccgggacagg tgcgcgaccc tgacgtgctc
      361 acccgttttg cggaacggtt cgaggacagc atgcgcatgc gctcggttgc acccaaggtc
      421 attttcggtg cggtcggtct catgggcacc gcgcgcaagg tcgtgcgcgg cgtcaccgac
      481 gccggcaagg atctgctggg cgcgctggac acggaggcgc aggacagcag gaagccgggg
      541 ccacccggcg gcgagtcgaa gtaaatggcc aagctccgca tcggccgcag ccaacggcgt
      601 cgtcaaagcg ccgtcaaact gctgcgcgag ctgcccaatc tgatcaagct gctggtccgc
      661 ctgctgcgcg acgcgcgcgt gtcacgcctg gaccgcgtgc tgttcggcgc cgtcatcgcg
      721 tatgtgctcc tgcccgccga cctgctgccg gatttcctgg gatttctcgg cctgacggac
      781 gacctgtacc tcattgggct ggccctgaat cggttgtttg cgcgcgccgg ggcagacgtg
      841 ctgctcgagc actgggatgg caatccgcgc gcgctgggct atctgatcga aggcgtggag
      901 gaagtcgggg gtttgctgcc tcggcgcatc cgtaacgcat tgcgcggact tatctaacgg
      961 cataaattcc gccatggaaa caccccgcaa ttctcgcatc ctcggcgaag ccctgacgtt
     1021 cgacgacgtc ctcctcgttc ctgattattc cgaagtacat ccgagcgcaa ccgacgtccg
     1081 atcgcggctg accaacagca ttgagctttc catcccgctg ctttcagcgg ccatggacac
     1141 cgtcacggaa tcacgcatgg caatcgcgat ggcgcgtgaa ggcggcattg gcatcattca
     1201 caagaacatg cccatcgagc ggcacgccag cgaggtggat cgggtcaagc gatcggagag
     1261 cgggatgatt cgcgacccga tcactttgag gccggacgaa acgctccagg acgcattgcg
     1321 cgtcatggag cgtttctcga tcagcggcgt gcccattgtg gaaaacggcg ggcgcctggt
     1381 cggaatcatc acgaatcgcg atctacaatt cgagacggcg ctgaaccggc cgatccgcga
     1441 agtcatgacg cgcgaaggat tgatcaccgc gcccatcggc acttccctgg aggacgccga
     1501 gcgcatcctg cagcggcatc gcattgaaaa gctgcccgtg gtcgatgctc agggaaatct
     1561 gcgcggcctg atcacggtga aagatatctt caagcggcgg caatttccga acgcctgcaa
     1621 ggatgagcac ggacgcctgc gcgtgggtgc ggcactcggc accgccgaga aagagctcga
     1681 gcgtgcggct gaattgctgg ccgcgggttg cgatgtcctg gtgatcgaca cggcgcacgg
     1741 gcactcggcc ggcgtgctcg atgcagtggc ccgcacccgc aatcgttttc cggatgcaca
     1801 gatcattgcc ggtaacgtgg caacggcgga cgccgccaaa gcgttggtgg agcgcggcgc
     1861 ggatgccgtc aaagtcggca tcggaccggg cagcatctgc accacgcgcg tcgtggccgg
     1921 cataggcgtt ccccagttga ccgccattat ggaatccgcg cgcggtgtgg acggccgtgt
     1981 gcccgtcatt gccgacggcg gtattcgcta ttccggcgac atcgtcaaag cgatcgctgc
     2041 cggcgctgca tccgtgatga tgggatcact gctcgcgggc acggaagaaa gcccgggcga
     2101 gagtttcctg ctcgaaggcc ggcgcttcaa gaccgtgcgc ggcatgggtt cgctctcggc
     2161 tatggccgaa ggctcagccg accgttactt ccaggagagc gtagccggca gtcgtaagct
     2221 cgtaccggaa ggcatcgaag gacgcgtgcc ctacaagggc cccgcctccg atgtggtctt
     2281 ccagctggtg ggcgggctgc gcagcggtat gggctactgc ggtacagcga atatcgaagc
     2341 actgcgcacg cgcgcgaagt tcgtgcgcat tacgtccggc ggattgcggg aatcgcaccc
     2401 gcatgatgtg gccattacgc gggaagcgcc caactaccac ctctaacggc aaacgtcgcg
     2461 acggaaaacc cggacggaga aagcgtggca gctccctgcc cgcagttcag tcccgctttt
     2521 ccgttacgcc gccccgccgt gctctgcgcc ttcaacgact aaaaccttga ctcaccccca
     2581 cttacgcgcc taccatagag gggtcccgtc tccccgtcac gggaaatcaa tcgttcccac
     2641 aactcaagag taagtaccgt ggcttccggt tccactacgg acgcgggcac cgtccaggtg
     2701 ttgcgcaccg aattagaggc gctcgagggc atcgagcgtg tggtcgttga agactccgca
     2761 atctggttca actgcgatcc agaggcggaa gtcagcgacc tgcgcacgcg cgcggaagag
     2821 gtgctgaagc gaaacggagt cgacgccgaa aagctgcagc tgcttttcct cactgatctc
     2881 tcggcacgcc gtatcaagtt ttcccgcatc gagcggatcg tggagcgcac cggcaacgtg
     2941 cgcatgcgcg tcgcgctgga atggcaggga ctggtcaagc tcggcgaggc cagtggagag
     3001 agcggtgatc tgctggaaca gcgcacggcc gcaacggcgg cactgattgc attggagaac
     3061 gtcgtcggtg agccgctcgg catcaagctg gtgggtgtga aacat
//
LOCUS       DASURG010000313         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1486541, whole genome shotgun sequence.
ACCESSION   DASURG010000313 DASURG010000000
VERSION     DASURG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486541"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFY80_05420"
     CDS             complement(<1..196)
                     /locus_tag="VFY80_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007379.1"
                     /translation="MWLALHFPFLAMDIFMRGTSVAVPLAVASSSAVGATLVSCNALA
                     HSRGVRNGMSVAAAWALASDL"
     gene            complement(221..907)
                     /gene="imuA"
                     /locus_tag="VFY80_05425"
     CDS             complement(221..907)
                     /gene="imuA"
                     /locus_tag="VFY80_05425"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEX6007380.1"
                     /translation="MASSAFNTPVHHPAVWRGAECAQVSVPSVPTGFAELDVLLPGAG
                     WPTETLTEIHVERPGIGEMMLLVPALARLSRSERWLAFIAPPYLPYGPALASHGIRLS
                     QFMLIRAQTAELQLWSGEQALRSGSCGAVLLWLDRVPERALRRLQLAAEGSGAFVMLF
                     RSARAPACSLAALRLYLSKADGRTIVRILKRRGGSVPAPVALDLHGAVTSRRTLSAVT
                     FPGACAHPAR"
     gene            complement(946..1551)
                     /gene="lexA"
                     /locus_tag="VFY80_05430"
     CDS             complement(946..1551)
                     /gene="lexA"
                     /locus_tag="VFY80_05430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041937182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6007381.1"
                     /translation="MGQELTSRQAEILEYIRSHAANSGYPPTRAEICRAMGFRSPNAA
                     EEHLRALARKGVIEMAPGASRGIRVTQTAGIPVIGRVAAGSPILAAPHIEAHYQLDPL
                     LFKPRADYLLKVRGASMRNAGILDGDLLVVHRTHEFRSGQIVVARLHDDVTVKRIHRQ
                     RHLVELLPENPEFRPITIDTRREPVVIEGIAVGVIRNGKPL"
     gene            1773..2204
                     /gene="rimP"
                     /locus_tag="VFY80_05435"
     CDS             1773..2204
                     /gene="rimP"
                     /locus_tag="VFY80_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX6007382.1"
                     /translation="MTDLHLLLETTLAGLGYELVDLERSGKGRLLRVFIDKAGGVDVE
                     DCATVSHHLSRLLPVEGVAYERLEVSSPGLDRSLKKERDFARFTGQKARIKLRVPIEG
                     QRNFVGVLRATRAGKVEIEIDGRVLSLDLDNLDKARLVPAI"
     gene            2219..3703
                     /gene="nusA"
                     /locus_tag="VFY80_05440"
     CDS             2219..3703
                     /gene="nusA"
                     /locus_tag="VFY80_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX6007383.1"
                     /translation="MSREILLLVDALAREKNVEKEIVFGALELALASAAKKRFNEDVD
                     VRAAVDRSTGEFKFFRRWEVVNDRDIETPSRQYKLHEAKEIDPENEVGQFIEEPIEGI
                     EVGRIGAQTAKQVILQKIRDAEREQILNDFLARKEFLVTGVIKRMERGNAIIESGRLE
                     ALLPRDQMIPKENLRVGDRARAYLWKIDRASRGPQLLLSRTAPEFLVKLFELEVPELE
                     DGLLEIKAAARDPGSRAKIAVKSNDQRIDPIGTCVGMRGSRVQAVTSELAGERVDIIL
                     WNPDPAQFVINALAPAEVSSIVVDEEKHSMDIVVDEENLAQAIGRNGQNVRLASELTG
                     WELNIMTVEESQRKTEEESSRVREIFMDKLDVDQEVADILVQEGFSTLEEVAYVPLNE
                     MLEIEAFDEATVNELRSRARNALLTQAIASEERVEHEIEDLMKVEGMDKETARLLASK
                     GVSTQEQLADYATDDLVETAGLEPERAKQLIMAARAPWFAETNA"
     gene            3714..>3926
                     /locus_tag="VFY80_05445"
     CDS             3714..>3926
                     /locus_tag="VFY80_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX6007384.1"
                     /translation="MAQINVTQFASELGLPPALLIEQLQAAGVKKALAEDTLLTEKDK
                     TQLLDYLRQVHGGRDAKQKITLTRRQT"
BASE COUNT          844 a         1133 c         1274 g          675 t
ORIGIN      
        1 gaagatcgga agcaagcgcc catgcggccg ccacgctcat accgttgcgt actccacgag
       61 aatgcgcgag cgcattgcag gagacgagcg tggcgccaac ggcgctcgac gaagcgacgg
      121 cgagcggtac agcaaccgag gtgccgcgca tgaatatatc catcgcaaga aaagggaagt
      181 gcagggccag ccacaacatg caaaactgcg ctttcggaaa tcagcgcgcc ggatgggcgc
      241 aagctccggg gaacgtgact gcagagagcg tgcggcgcga ggtcactgca ccgtgcaaat
      301 cgagcgcaac cggtgccggc acgctgcctc cgcgccgttt gagaatccgc acgatcgttc
      361 tcccgtccgc cttgctcagg tacaggcgca acgccgcgag cgagcaggcc ggtgcgcgcg
      421 cgctgcgaaa caacatgacg aatgcgccgc tgccttcggc ggcaagctgt agtcggcgta
      481 acgcgcgctc cggcacgcga tcgagccaca ggagcaccgc accgcagctg ccggaacgca
      541 atgcctgctc gcctgaccaa agctgcaatt ccgcagtttg cgctcgaatc agcatgaact
      601 gcgacaggcg aatcccatga cttgcaagcg ccggtccata cggcaggtag ggcggcgcga
      661 tgaaagcgag ccaacgctcg ctgcggctca ggcgtgcgag cgcaggcacc aaaagcatca
      721 tttcaccgat gcccggacgc tcgacgtgaa tttcggtgag cgtttccgtc ggccagcctg
      781 cgcccggcag cagcacgtcg agctcggcaa aaccggtcgg tacgctggga acggaaacct
      841 gcgcacattc ggcgccgcgc cagacggcgg ggtgatggac gggcgtgttg aaagcgctgg
      901 aggccacggc aggcatcgga tcagaacgat gaccggaaat cgctgctaga gcggcttgcc
      961 gttgcgaatc acgcctaccg caatgccttc gatgacgaca ggttcgcggc gggtatcgat
     1021 ggtgatcggg cggaattccg gattctcggg aagaagttct acgaggtgac gctggcggtg
     1081 gatgcgcttc accgtcacgt catcgtgcaa acgcgcaacc acgatttgcc ccgaccggaa
     1141 ttcatgcgtg cgatggacga ccagcaggtc gccgtcgagg atacccgcgt tgcgcatgct
     1201 tgctccacga actttcagca gataatcggc gcgcggcttg aaaagcaggg gatcgagctg
     1261 atagtgcgct tcgatgtgcg gggcagcgag gatggggctg ccggccgcta cgcgtccgat
     1321 taccggaatc cctgcggtct gggtcactcg aatgccgcgc gaggcgcccg gcgccatctc
     1381 gatcacgccc ttgcgcgcca aggctcgcag atgctcttcc gccgcattgg gcgatcgaaa
     1441 tcccatcgcc cggcaaattt cggcccgcgt aggcggatat cccgaattcg cggcgtgact
     1501 gcggatgtat tccagtatct cggcctgacg tgaggtgagt tcctgcccca tgcctgtaat
     1561 tatatacagt atatgaggca tggcaagcct tctttgccga tgccctgccg actccaaaaa
     1621 taaactgcac ccgaaaatcg tttgccaccg gcgggcggcg cgcgctcaaa agccgcacgg
     1681 tcttggattg cgcttcgtat ttttgttata cttcaaagct ctgtgagaag aagatgggcg
     1741 gttcgcccat tttttgtttg tggcaacgat acatgacgga tttgcaccta ctcctcgaga
     1801 ccaccctcgc cgggcttggc tatgaactcg tcgatcttga aagatcgggc aaggggcggc
     1861 tcctgcgcgt atttatcgac aaggccggtg gcgtggatgt ggaggattgc gcgaccgtga
     1921 gccaccacct cagccggctg cttccggtgg aaggcgtggc ttacgaaagg ctcgaggtct
     1981 cctcaccggg actcgacagg tcgctcaaga aggaacgcga ctttgcgcgc ttcaccggcc
     2041 agaaggcgcg catcaaactg cgcgtgccga ttgaagggca aaggaatttt gtcggtgttt
     2101 tgagggcaac gcgtgcagga aaggtcgaga tcgaaatcga cggtagggtg ttgtcactcg
     2161 atctggataa cctggacaag gcgcggctgg tgcccgcaat ttagcaggag agattgagat
     2221 gagccgcgag atattgttgc tggtggacgc cttggcgcgc gagaagaacg tcgagaagga
     2281 gatcgttttc ggcgcgctcg agctcgcgct cgcttccgcc gcgaagaagc gtttcaacga
     2341 agacgtggac gtgcgcgccg cggtggatcg tagcaccggc gagttcaagt tcttccggcg
     2401 ctgggaggtg gtcaacgacc gggacatcga aacgccctcg cgccagtaca agctgcatga
     2461 agcgaaggag atcgatcccg agaatgaagt cggccagttc atcgaggagc cgatcgaagg
     2521 catcgaagtc ggtcgcatcg gcgcgcagac cgccaagcag gtgattctcc agaagatccg
     2581 cgacgccgag cgcgaacaga tcctgaatga ttttcttgcc cgcaaggagt ttctcgttac
     2641 cggcgtgatc aagcgtatgg agcgcggtaa tgcgatcatc gaatcgggcc gcctggaagc
     2701 gctgctgccg cgcgatcaga tgatcccgaa ggaaaatctg cgtgtgggcg atcgtgcccg
     2761 cgcgtatctt tggaagatcg accgggcgag ccgtggcccg cagctgcttc tgtcgcgcac
     2821 tgcgccggaa tttctcgtta agcttttcga gctggaggtg ccggagctcg aggacggtct
     2881 gctcgaaatc aaggctgccg cgcgcgatcc cggttcacgc gcgaagatag cggtgaagtc
     2941 gaacgatcag cgcatcgacc ctatcggtac ctgtgtcggc atgcgcggtt cgcgcgtgca
     3001 ggcagtgacg tccgagcttg ccggagagcg tgtcgacatc atcctgtgga atccggatcc
     3061 tgcgcagttc gtcattaatg cgctggcgcc ggcggaggtg agcagcatcg tcgtggacga
     3121 agaaaaacac agcatggaca tcgtcgtcga tgaggagaac ctcgcccagg ccatcgggag
     3181 gaacgggcag aacgtaagac tcgcgagcga gctcacgggc tgggaactca acatcatgac
     3241 ggtggaagag tcgcagcgga agaccgagga ggaatcgagc cgcgtccgcg agatcttcat
     3301 ggacaaactc gacgtcgatc aggaagtcgc cgacatcctg gtgcaggaag gattctccac
     3361 gctggaagag gtggcttacg tgccgctcaa cgagatgctc gagatcgaag cgttcgacga
     3421 ggcgaccgtg aacgaattga ggagccgcgc gcgcaatgcg ctccttactc aggccattgc
     3481 gtccgaagag cgggtcgagc acgaaatcga ggacctgatg aaggtcgagg gcatggacaa
     3541 ggagacggcg cgccttctcg cgtcgaaggg cgtcagcact caggagcagc ttgccgacta
     3601 cgcgaccgac gatcttgtcg aaaccgcagg cctcgagccg gagcgcgcga agcagctcat
     3661 catggcggcg cgagcaccgt ggttcgcgga aaccaacgca taagcaggaa taaatggcgc
     3721 agatcaacgt cactcaattt gccagcgaac tggggcttcc ccccgctctg ctcatcgagc
     3781 agctgcaggc ggccggcgtg aaaaaagcgc tggccgaaga cacgctgctc accgaaaagg
     3841 acaagacgca gcttctcgac tacctgcgtc aggtgcacgg cggaagggac gcgaagcaga
     3901 agatcacgct cacccgccgt cagacg
//
LOCUS       DASURG010000314         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1486550, whole genome shotgun sequence.
ACCESSION   DASURG010000314 DASURG010000000
VERSION     DASURG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486550"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFY80_05450"
     CDS             complement(<1..279)
                     /locus_tag="VFY80_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX6007385.1"
                     /translation="MTGSPTRLRAWQALQDHHRVAAARHLRELLADPQRFAKYSFACG
                     DILFDFSKHRIADDTLDLLIALAQECDVPGWIARLFAGERVNTTEHRAA"
     gene            468..1187
                     /locus_tag="VFY80_05455"
     CDS             468..1187
                     /locus_tag="VFY80_05455"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX6007386.1"
                     /translation="MADIVMALGAALRDLRAPRILAVMLLPMLGAILIWTLLSFIFWD
                     SWSAWLTALTADTAAGRWLRDVGAGWLLHALNILGAIALVIPATLVTALVINEIVVMP
                     AIVTHVGARYFPGLERRAGGTLAGSIANAVIAITIFCVLWVLTLPLWLTGVGAVVLPA
                     LISAYLNQRLLRYDALSEYAARDEYAAIIAGSKGKLYALGFLVALLYYVPIVNLLAPV
                     ASGLAFTHLCLAELARVRRAA"
     gene            complement(1196..2386)
                     /locus_tag="VFY80_05460"
     CDS             complement(1196..2386)
                     /locus_tag="VFY80_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HEX6007387.1"
                     /translation="MNTGREVVVLSGVRTAIGDYGGALKDVAPTELAAQVVREAVKRA
                     RVDPQQVHQMVFGNVIHTEARDMYFSRVACIKGGLPQDTTALTVNRLCGSGLQAIVSA
                     AQAIQLGDADVAVGGGAESMSRGAYIVPALRWGQRMNDGGVVDMMVGALTDPFDTVHM
                     GITAENISEKWKITREQQDEFAVESHRRAVNAIEKGYFKDQILPVELKTRKGTTVFER
                     DEHPRADASLEGMAKLRAAFRKEGTVTAGNASGINDGAAAVVLMERNAAEKAGLKPMA
                     RLVSYGLAGVDPKIMGIGPVPACQKALARAGLKVDDMDVVESNEAFAVQAMAVSCDLK
                     FDPKKTNPNGGAVALGHPIGATGAVLTVKALYELQRIGGRYALVTMCIGGGQGIAAVF
                     ERVH"
BASE COUNT          375 a          867 c          812 g          508 t
ORIGIN      
        1 cgccgcacgg tgctcggtcg tgttgacgcg ctcgcccgcg aaaagtcgcg cgatccagcc
       61 gggcacgtcg cattcctgcg ccagcgcaat caagagatcg agcgtatcgt ccgcgatgcg
      121 atgcttcgaa aaatcgaaga ggatgtcgcc gcaggcgaac gaatacttgg cgaagcgctg
      181 tggatcggcg aggagctcgc gcagatggcg agccgcggcg acgcggtgat gatcctgcag
      241 cgcctgccag gcccgcagtc gggtggggga gccggtcatc gcggtggcag cgcctcccgg
      301 cgcgggtttc gggatactcg gcattctacc tttccggatc gcgttaccgg cgttcgcacg
      361 gttacaatga agaacgccaa cttcacggaa agttctccat gcatggggcg cgccgcctca
      421 ttcccctttc gcgcttcggc acccggacga cgtgaggttg caccgcgatg gctgacatcg
      481 tgatggcgct cggcgccgct ctgcgcgacc tgcgcgcgcc gcgcatactt gcggtcatgc
      541 tgctgcccat gctgggcgcc attttgatct ggaccctcct gtccttcatc ttctgggaca
      601 gctggtccgc ctggctcacc gcgcttaccg ccgacactgc ggcgggccgg tggcttcgag
      661 acgtgggcgc cggctggctg ctccacgcgc tcaacatcct cggcgccatt gcgctcgtca
      721 tcccggcaac cctcgtcacc gcgctcgtga tcaacgagat cgtggtcatg ccggcgatcg
      781 tcacgcacgt gggcgcgcgc tatttccccg gcctcgagcg gcgcgccggc ggcacgcttg
      841 ccggcagcat cgcgaacgcg gtcattgcga tcacgatatt ctgcgtgctc tgggtgctga
      901 cgcttccgct gtggctcacg ggcgtgggtg cggtcgtgct tcccgcgctc atctcggcgt
      961 atctcaatca gcggcttctt cgttacgatg cgctttccga gtacgcggcc cgggacgagt
     1021 atgcagctat catcgcgggc agcaagggca agctgtacgc gctcggtttc cttgttgcac
     1081 tcctctatta cgttccgatc gtgaacctgc tcgcgccggt ggcgagcggg ctcgctttta
     1141 cgcatctctg tctcgcggaa cttgcgcgcg tgcggcgagc ggcctgaagc cgctttcagt
     1201 gcacgcgttc gaacacggcg gcgatgccct ggccgccgcc aatgcacatg gtgacaagcg
     1261 cgtagcggcc gccgatccgc tgaagctcgt aaagcgcttt cacggtcaac accgcgccgg
     1321 tcgcgccgat cgggtgaccc agggctacgg cgcctccgtt gggattggtt ttcttcgggt
     1381 cgaacttgag gtcacaggaa accgccatcg cctgcaccgc aaacgcttcg ttcgattcga
     1441 cgacgtccat gtcatcgacc ttgaggccgg cccgggcgag cgccttctgg cacgccggaa
     1501 cgggcccgat gcccatgatc ttcgggtcca cgccggcgag gccgtaggag acgagccggg
     1561 ccatcggctt gagccccgct ttctccgccg cgttcctttc catcaacacg accgcagcgg
     1621 cgccgtcatt gatgcccgag gcgttgcctg cggtcaccgt gccttccttc ctgaacgccg
     1681 cacgcagttt cgccatgcct tcaaggctcg catcggcgcg cggatgctcg tcgcgctcga
     1741 atacggtcgt gcctttgcgc gtcttcagct ccaccgggag gatctggtcc ttgaaatagc
     1801 ccttttcgat ggcattgacg gcgcgccggt ggctctccac ggcgaactcg tcctgctgtt
     1861 cgcgcgtgat tttccatttt tcggaaatgt tttcggcggt aattcccatg tgcaccgtgt
     1921 cgaacggatc ggtcagcgcc ccgaccatca tgtccactac gccgccgtcg ttcatgcgct
     1981 gaccccaacg cagcgccggc acgatatagg cgccgcggct catcgattcc gcgccgccgc
     2041 cgaccgcgac atccgcatcg ccgagctgga tcgcctgtgc ggcgctcact atggcctgga
     2101 gaccgctgcc gcacaggcgg ttcacggtga gcgcggtggt gtcctggggc agcccgccct
     2161 tgatgcacgc gacgcgcgag aaatacatgt cgcgcgcctc ggtgtggatc acgttgccga
     2221 acaccatctg gtggacctgc tggggatcga cccgggcgcg cttgaccgct tcgcgcacga
     2281 cttgtgcggc aagctcggtc ggcgccacgt ccttgagtgc gccgccgtag tcgccgattg
     2341 cggtgcgaac gccgctcaag accaccactt cacgacctgt gttcatggct ttctccttga
     2401 tctgcaggga tgaatacgga aacgggcggg cgccgccggg cgacaccgac tgggcagacg
     2461 agtttacgct cgggctcgcg ccgttgtcga gtccacggcc cgggcggcgg ctcagcgctt
     2521 tcccgtgctc tgatagagac ggtagcgctc gtccttgtca cc
//
LOCUS       DASURG010000315         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_770019, whole genome shotgun sequence.
ACCESSION   DASURG010000315 DASURG010000000
VERSION     DASURG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770019"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..178
                     /locus_tag="VFY80_05465"
     CDS             14..178
                     /locus_tag="VFY80_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX6007388.1"
                     /translation="MLSWAVAFFVIALIAALFGFTGIAGTAVNIAYILAVIGIVLAIV
                     FLIIGRRPPV"
     gene            282..1142
                     /locus_tag="VFY80_05470"
     CDS             282..1142
                     /locus_tag="VFY80_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6007389.1"
                     /translation="MERLRRVRDAVPGLRVFVFAFVNYIRHQSANQAGSVAFSAVLAS
                     CPLLLFLSAAAAYIGQPGAAAELARRILSFAPPAVADTLQPVVDEVLSQRNRALVTVG
                     IFFTIWAASSGIQAIRTALNKAYGVEQGLAFWSARIKVIIFTVIGTVGTVLAFASVVV
                     MPYVWLALDEAVADGGESRWTRDAVRYGIAYLVLTALYAAMYGWLPDIRQRLWTVLPG
                     ALVGALLWLGAATLLSYTLRSIGELALVYGSFAGLVATLVFLYVSAVTLIFGAEINGV
                     LRSGAPPKQA"
     gene            1250..>2127
                     /locus_tag="VFY80_05475"
     CDS             1250..>2127
                     /locus_tag="VFY80_05475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6007390.1"
                     /translation="MSETATQTQVAMPDKVVTLPAPATPRRLKDILCLDDFEAPARRH
                     LPRPIFGYISGGVETNASLAGNRAAFAEWEFLPRILCNTRARHQRTTLFGRTYDLPFG
                     FPPMGGTSLAAYMGDSVLARVAAELNTVMIQSGASLTPMERVREVGPTAWFQAYLPGD
                     PEIIGPLVERAQRAGFEVLVLTVDVQVTANRENNVRAGYSSPLKPTPRLAWDCMVRPR
                     WLLGTFGRTLMNHGMPHLENMGFPRLPIIGNIERPRMARDGLCWEHLELMRKMWKGKL
                     VLKGVLSPRDVRIARE"
BASE COUNT          329 a          738 c          652 g          408 t
ORIGIN      
        1 tggaggactc gatatgcttt cctgggccgt cgcttttttc gtcatcgcac tgatcgccgc
       61 gctcttcggc ttcaccggaa ttgccggcac cgctgtcaac atcgcctaca tcctcgcggt
      121 gatcggcatc gtgcttgcga tcgtgtttct catcatcgga cgccgcccgc cggtataaca
      181 ccaggcttcg cctcgaacgt tctgcaccga caaccactcc aacggggcac accggaacgc
      241 tcaattcata aagacacgcc gtgaacccgt ggcggggttt cgtggaaaga ttgcgccgcg
      301 tccgcgatgc cgtgcccgga ctgcgggtgt tcgtgttcgc gttcgtgaac tacatccgcc
      361 accagagcgc caaccaggcg ggcagcgtcg cattctcggc cgtgctcgcg tcctgtcctc
      421 tcctgctctt tctttccgcg gccgccgcct acatcggcca gcccggcgcc gccgccgaac
      481 tggcgcgccg gatcctctcg tttgcgccgc cggccgtcgc cgatacgctg cagcccgtgg
      541 tcgatgaggt cttgagccag cgcaaccggg cgctcgtaac cgtcggcata tttttcacca
      601 tctgggccgc ctcctccggc atccaggcga tccgcaccgc gctcaacaag gcatacggtg
      661 tcgagcaggg gctcgccttc tggagcgcac gcatcaaggt catcatcttc accgtgatcg
      721 gcacggtggg gaccgtgctt gccttcgctt ccgtggtcgt catgccctat gtgtggctcg
      781 cgctggacga ggccgtcgcc gacggcggcg aaagcaggtg gacgcgcgac gctgtgcgct
      841 atggcatcgc atatctggtg ctcactgcgc tctatgccgc gatgtacggc tggctgcccg
      901 atatccggca gcggctttgg accgtgttgc ccggtgcgct cgtgggcgcg ctgctgtggc
      961 tcggcgcggc aacccttctt tcttacaccc tgcgcagtat cggcgaactt gcgctggtct
     1021 atggcagctt cgccggtctg gtcgcaacgc tggtcttcct ctacgtgagc gcggtcacgc
     1081 tgattttcgg cgccgagatc aatggcgtgc tgcgcagcgg cgcccccccg aagcaggcgt
     1141 aggccggtcg atccccgcca gcaagtccat aatgcggcca tgatgccggt ccccgcagcc
     1201 tcgctgagcg cactcactta taatcgctgc ggttcaccgg agagcactca tgagcgaaac
     1261 cgccacgcaa acccaggtcg cgatgcccga caaggtcgtg acgctaccgg cgcccgccac
     1321 gccgcgccgg ctgaaggaca tcctgtgcct ggacgatttc gaagcgcccg cgcgccgcca
     1381 tctgccccgc ccgatcttcg gttacatctc cgggggcgtg gagaccaatg cctcgctcgc
     1441 gggaaatcgc gccgcgtttg ccgaatggga atttctgccg cgcatcctct gcaatacccg
     1501 cgcgcgccac cagagaacga cgcttttcgg ccgcacctac gatctgccat tcgggtttcc
     1561 acccatgggc ggcacttcgc ttgcggccta catgggtgat tcggtactcg cgcgggtcgc
     1621 ggcagagctc aacaccgtga tgatccagag cggcgcctcg ctcacaccga tggagcgcgt
     1681 gcgcgaagtg ggcccgaccg cctggttcca ggcttacctt cccggcgatc ccgagatcat
     1741 cgggccactg gtggagcggg cgcagcgcgc cgggttcgaa gtgctggtgc tcaccgtgga
     1801 cgttcaggtc accgccaacc gcgaaaacaa cgtgcgcgcg ggctacagct cgccgctcaa
     1861 acccaccccg cgactcgcat gggactgcat ggtgcggccg cgctggctcc tcggcacctt
     1921 cggccgcacg ctgatgaatc acggcatgcc gcatttggag aacatgggtt ttccgcgcct
     1981 tcccataatc ggcaacatcg agcgcccgcg catggcgcgc gatggcctgt gctgggagca
     2041 tctggagctg atgcgcaaga tgtggaaggg caagctcgtt ctcaaagggg tgctctcgcc
     2101 ccgcgacgtg cgcatcgccc gcgagag
//
LOCUS       DASURG010000316         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_260638, whole genome shotgun sequence.
ACCESSION   DASURG010000316 DASURG010000000
VERSION     DASURG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260638"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(609..887)
                     /locus_tag="VFY80_05480"
     CDS             complement(609..887)
                     /locus_tag="VFY80_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007391.1"
                     /translation="MSDNVELTAEADDFFRTLPGDAYPQALVDQFPRIANTIVELRYD
                     REKLGEYFKSLLNDSRGGRQGFPFPVLMNLQNLHDAMLGTETGSTFWV"
     gene            1063..1731
                     /locus_tag="VFY80_05485"
     CDS             1063..1731
                     /locus_tag="VFY80_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX6007392.1"
                     /translation="MRVFSTGGTRVLVLLVSFAVAAFAAEIVSLTDAQIAKLAQQFGP
                     IAKTRLTAWREILTAPKYKKLPEEEKLRVVNDFMNETRFVSDREHWGKEDYWATPIEF
                     LSTNGGDCEDYSIAKYFTLRALGVPDDKLRITYVKELVIYNEAHMVLAYFPTPDAEPL
                     VLDNINKTIQPASNRNDLLPVYSFNGSGLWLAKEQTGRGQSVGGSDRIGHWRDLQARL
                     RAVM"
     gene            1767..>2908
                     /locus_tag="VFY80_05490"
     CDS             1767..>2908
                     /locus_tag="VFY80_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HEX6007393.1"
                     /translation="MKLSRLLVSLISLLWALVFFATLAIVVNSTQDYLQKSMESHAQD
                     TATSLGLSITHSGRVKDPQMVERMTAAIFDRGYYTEILVKSMDGKEIVAKRVEQAVKG
                     VPEWFIAYFPLETPRMEAIVMDGWRQAARIEVVSHPGHAYAELWRVAVRSFWVLLVVA
                     IVSLIVVLVVLQRALRPLDDMEAQAIAISRRQFTVLRKLPWARELHRVANAMNNMCLA
                     VERMLTEQTDLAEKMRKKAYVDPVTGLMNRNDFSEQVAHLVTAPTKFPSGALAVVRIR
                     GFASYNERNGRVEGDALLKRTAHLLSKALERHEAPTLAKLDGPEFGILMPNIPQLDLP
                     RLGDEIIAALAEIEEFPHTDTSVMAHAGIVYYRHHDGASFGKLMATA"
BASE COUNT          574 a          865 c          925 g          544 t
ORIGIN      
        1 acggatgcta attcgtcgcc atatagcgac aacaagatta aaaaacgtag tccgggcggc
       61 tcgcggttgc gctcgctgga cgagctttag ctgtaggacg gaatcggtat agaaaggccg
      121 ataaggggag gatcgtggtg ctttcggcgc gcattgaggc gcaatgccgc gcgatgatgg
      181 tccgactacg cgtaagccaa acggcctgtc gggaaacaac gacgcgatgt cgagagaatt
      241 acttctgcaa tgaccggatt ggcgtacttg aggcgacacg ctattcattc gtcattgtcg
      301 ttgaatagcg acgacatgtc gcggaagcgt gtcgggaagt cgattgaccg gctttgggga
      361 taactggtac accacagacg tcggtccgtg ggcatttgaa cgcggcactc cctgatgcga
      421 ctgcggacat cggccgcaaa ccttttcgtg gtcttcgtaa gcgaccgacg ggtgcaggct
      481 cgtgttcgcc gctcgagcta cggccgtcgg ccgtggtgct acgtcgcccg cccgatcata
      541 gccaacgcct agaaattaag caacggaaag tgaaacgccg gcccaccgag cgtcgcaggg
      601 aagaggtgct agacccagaa cgtggagcca gtttccgtcc cgagcatcgc gtcgtgcagg
      661 ttctgcaggt tcatgaggac ggggaaaggg aaaccctggc gcccgccccg gctgtcgttt
      721 agcagggact tgaaatactc ccccagcttc tcgcggtcat agcgcagctc gacgatggta
      781 ttcgcgatgc ggggaaactg gtcgaccagc gcttgcgggt aggcgtcccc aggcagcgtc
      841 cgaaaaaaat catccgcttc cgccgtgagc tcgacgttat ccgacatttt tctttcctct
      901 ctcttgccaa gagcaatcgt tcgcgaagaa gtttagacca gcggtccgcg catcgcaatg
      961 acgtgatcgc ggcggaaaac tccggtatca tccggcgagc cacaggggcg ggcgttggga
     1021 gggtccggaa atgccggatt cggcgtccag gcaggggagg gtatgcgggt attttccacc
     1081 ggcggcacgc gcgtactcgt gctgctcgtg tcttttgcgg tcgcggcgtt cgcggccgaa
     1141 atcgtttctc tcaccgacgc gcagatcgcc aagctcgccc agcagttcgg ccccatcgcg
     1201 aaaacgcgcc tcaccgcatg gcgcgagatc ctcaccgcgc cgaagtacaa gaagctcccc
     1261 gaggaggaga agctgcgggt cgtgaacgat ttcatgaacg agacccggtt cgtgagtgac
     1321 cgtgagcact ggggcaagga agactactgg gcgacgccga tcgagttcct gtcgacgaac
     1381 ggcggggatt gcgaggacta ttcgatcgcc aagtatttca cgctgcgcgc gctcggcgtg
     1441 ccggacgaca agctgcgcat cacctatgtg aaggagctcg tgatctacaa cgaggcgcac
     1501 atggtgctcg cctattttcc gacgccggat gccgagccgc tggtgctcga caatatcaac
     1561 aagacgatcc agccggcttc gaaccgcaac gatcttctgc ccgtgtacag tttcaacggc
     1621 tcgggtctgt ggctggcgaa ggagcaaacc ggccgcgggc aatcggtcgg cggctcggac
     1681 cgcatcgggc actggcgcga tttgcaggca cgtctgcggg cggtgatgtg acgagcaact
     1741 gaagaacact aagaacaggg ggcgggatga aactctcacg actactggtt tcgctcattt
     1801 cgctgctgtg ggcgctcgtt ttcttcgcca cgctcgcaat cgtcgtcaac agcacccaag
     1861 actacctcca gaaatcgatg gagtcgcatg cgcaggatac cgccacttct ctcggccttt
     1921 cgattacgca cagcggccgc gtcaaggatc cgcagatggt cgaacgcatg accgccgcca
     1981 tcttcgatcg cggttactac accgagatcc tggtgaagag catggacggc aaggaaatcg
     2041 tcgcgaagcg cgtcgagcag gcggtcaagg gtgtgcccga gtggttcatc gcctacttcc
     2101 cgctcgaaac gccgcgcatg gaggcgatcg tcatggacgg ttggcgtcag gctgcgcgga
     2161 tcgaggtggt gagtcatccc gggcacgcct atgccgagct gtggcgcgtc gcggtgcgct
     2221 cgttctgggt gctgctcgtc gtcgcaatcg tgtctctcat cgtcgtgctc gtcgtgctcc
     2281 agcgcgcgct gcgcccgttg gatgacatgg aggcccaggc aatcgccatc tccagacggc
     2341 agttcacggt gctgcgcaag ctcccgtggg cgcgcgagct gcaccgcgtg gcgaatgcga
     2401 tgaacaacat gtgcctggcg gtggagcgca tgctgaccga gcagaccgat ctcgcggaga
     2461 aaatgcgcaa gaaggcctac gtcgatccgg tgacgggcct catgaatcgc aacgacttca
     2521 gcgagcaggt ggcgcatctc gtcaccgcgc cgaccaagtt tccgtccggc gcgctcgccg
     2581 tcgtgcgcat ccgcggtttc gcctcgtaca acgagcgcaa cggccgcgtc gagggcgatg
     2641 ccttgctcaa gcgcacggcg catcttctgt cgaaggcgct cgaacgacac gaggcgccga
     2701 ccctcgcgaa gctcgacggg ccggagttcg gcatcctgat gcccaatatt ccgcagctgg
     2761 acctgccgag actcggcgac gagatcattg cggcgctcgc cgaaatcgag gagtttccgc
     2821 acaccgatac gagcgtcatg gcgcacgccg ggatcgtcta ttaccgccac cacgatggcg
     2881 cgtcgttcgg gaagctgatg gctaccgc
//
LOCUS       DASURG010000317         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1487351, whole genome shotgun sequence.
ACCESSION   DASURG010000317 DASURG010000000
VERSION     DASURG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487351"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFY80_05495"
     CDS             complement(<1..222)
                     /locus_tag="VFY80_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX6007394.1"
                     /translation="MRGWDAARNVLCVRLDSMGDVLMTSPAMRALRESHRARRLTLLT
                     SRSGATVAPHIPEVDAVIEFTAPWMKAAGC"
     gene            complement(237..830)
                     /locus_tag="VFY80_05500"
     CDS             complement(237..830)
                     /locus_tag="VFY80_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6007395.1"
                     /translation="MALSRNGRAVFLDKDGTLIENVPYNVDPARVRFTPGAIEGLRLL
                     ADRGYRLFVVSNQPGVALGRYSEDALDAVARHIRDQLLREGIDLEGFYYCPHLPQGAI
                     PRYAIACACRKPASGLIERAARDHGIELTRSWMVGDILDDIEAGRRVQCRTILIDNGN
                     ESEWNLDGERRPEALAGDLLSAARIILASGAGSRSPS"
     gene            complement(821..1546)
                     /locus_tag="VFY80_05505"
     CDS             complement(821..1546)
                     /locus_tag="VFY80_05505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6007396.1"
                     /translation="MKSLKGRVAIVTGGGRGLGAAISRTLAAEGTHVAIADLDIARAR
                     ALATSLEHDGLASTAMKLDVRSEADVESCVGEVAQRYGRVDIVVNNAAVDYTLPLEEL
                     RVAQWDAVIATNLRGPFLLSKQASTVMLKQGSGHIINIASTASRRTWPNASAYHASKW
                     GLLGLSHALHAELRPRRIKVSAIIAGGMRTPFLLDRFPDIDPGVLQDPADVAEAVKFV
                     LCQPDETVIPEVMVIPMRETSWP"
     gene            complement(1543..2853)
                     /locus_tag="VFY80_05510"
     CDS             complement(1543..2853)
                     /locus_tag="VFY80_05510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6007397.1"
                     /translation="MTRKIAIISDHASPLAIAGGNDSGGQNVYVASIARHVQSMGCAV
                     DIFTRRDNPTLADVVELTSGLRVVHVRAGPEEALRKEALLPGMPEFTSGVAEFCRAHG
                     RYDVVHANFFMSGLAALELKTRFGIPFVVTFHALGRVRRMHQREADQFPDERIDIEDR
                     TVEQASAIIAECPQDCVDLISLYGAERDKIRIVPCGFDTREFWPVRRSFARRLLGYRE
                     DERIVLHVGRIVPRKGVDNVIRALGRLARSSDVRPRLIVVGGNSDAPDCAATPELARL
                     TDIAREEGVLAQTVFTGRRSREVLKLYYSAADVFVTTPWYEPFGITPLEAMACGTAVI
                     GSAVGGIKHTVADGVTGYLVPTNDPDALAERLAHLYRNPALLARLGANGVRRVHAQFT
                     WERVAKALAAVYEQVAPRHAVAYAERRRINAARAYRQPSGVPAR"
BASE COUNT          557 a         1048 c          990 g          545 t
ORIGIN      
        1 gcatcccgcg gctttcatcc acggcgcggt gaactcgatc acggcgtcca cttcgggaat
       61 atgaggcgcg accgtggcgc ccgaacgtga agtgagcagc gtaaggcgac gcgcgcggtg
      121 cgactcgcgc aacgcgcgca tggccgggct cgtcatgagc acatccccca tgctgtccag
      181 tcgcacgcac agcacgttac gcgcggcgtc ccagccgcgc atgggtgcgc gaggcctcac
      241 gatggcgagc ggcttccggc gccgctcgcg agaatgatcc tcgcggccga caggagatcg
      301 ccggcgaggg cttcgggtct gcgctcgccg tcgagattcc attcgctttc gttgccgttg
      361 tcgatgagta tcgtgcggca ctgcacgcgg cgcccggctt cgatatcgtc caggatgtcg
      421 cccaccatcc acgagcgcgt aagctcgatg ccgtgatcgc gcgccgcacg ctcgataagc
      481 ccgctcgccg gcttgcggca tgcgcacgcg atggcatagc gcggtatggc cccctgcggc
      541 aggtgcgggc agtagtagaa accctcgagg tcgataccct cgcggaggag ctgatcgcga
      601 atgtgacgcg ccacggcatc gagcgcgtcc tcggaatagc gcccgagcgc aacgccgggc
      661 tgattcgata cgacgaaaag ccgatatccc cggtctgcga gcaggcgcaa gccctcgatc
      721 gcccccggcg tgaagcgaac gcgggccgga tcgacgttgt acgggacgtt ctcgatcagg
      781 gtgccatcct tgtccaggaa cactgcgcgc ccgttgcggc tcagggccat gacgtttcgc
      841 gcatgggaat caccatcacc tcgggaatga ccgtttcatc cggctggcac agcacgaact
      901 tcaccgcttc ggcgacgtcg gcggggtcct gcagcacccc ggggtcgatg tccggaaacc
      961 ggtcaagaag aaacggtgtt ctcatgccgc ccgcgatgat cgcgctcacc ttgatccggc
     1021 gcggcctgag ctccgcgtgc agggcgtgac tcagcccgag cagtccccac ttgctcgcgt
     1081 ggtaagccga ggcgttgggc cacgtgcggc gcgatgcggt ggacgcgatg ttgatgatgt
     1141 gaccactgcc ctgtttcagc atgaccgtgc tcgcctgctt agacaggaga aaaggccccc
     1201 gcagattggt cgcgatcacg gcgtcccact gtgcgacgcg cagttcctcg agcggtagcg
     1261 tatagtccac cgccgcgtta ttgacgacga tatcgacgcg accgtagcgc tgcgcgacct
     1321 cccctacgca cgactccaca tccgcttcgc tgcgcacatc gagcttcatt gcagtcgagg
     1381 ctaggccatc gtgttcaaga ctggtggcaa gcgcgcgtgc gcgcgctata tcgaggtcgg
     1441 cgattgcgac gtgcgtaccc tcggctgcca gtgtccgcga aatcgcggca cccaggccgc
     1501 gcccgccgcc cgtcacgatc gctacacggc ccttgaggct cttcatcgcg ccggcactcc
     1561 gctcggttgc cggtaagcgc gcgcggcatt gatgcgcctg cgctcggcat aggcgacggc
     1621 gtgtcgcggc gccacctgct cgtacactgc cgccagcgcc ttcgccacac gctcccaagt
     1681 gaattgcgca tgcacgcgcc tcacaccgtt tgcgcccagt ctggcaagca gcgcgggatt
     1741 gcggtaaaga tgggcaagac gttcagcaag cgcatccgga tcgttggtcg gaacgagata
     1801 acccgtcacg ccgtcagcga cggtgtgctt gatgcccccc accgcggaac cgatgaccgc
     1861 cgtgccgcac gccatcgcct cgagcggtgt aatgccgaag ggttcgtacc atggtgtcgt
     1921 gacgaagacg tccgcggcac tgtagtagag cttgagcact tcgcggctgc ggcggccggt
     1981 aaatacggtc tgcgcgagca cgccttcttc gcgcgcgata tccgtcagcc gggcgagctc
     2041 cggcgtcgcg gcgcagtccg gcgcatccga atttccgccg accacgatca gccgcggacg
     2101 cacgtcgctc gaacgcgcga ggcgcccgag cgcgcggatg acattgtcca cgcccttgcg
     2161 cggcacgatg cgcccgacat ggagcacgat gcgctcgtct tcgcggtaac cgaggagtct
     2221 gcgcgcgaag cttctgcgca ctggccagaa ttcccgcgta tcgaagccgc acggaacgat
     2281 gcggatcttg tcgcgctctg cgccgtagag cgaaatgaga tcgacgcagt cctgcgggca
     2341 ttcggcgatg atcgcgctcg cctgctcgac cgtgcgatcc tcgatatcga tacgctcgtc
     2401 cggaaactgg tccgcctcac gctgatgcat gcggcgtacg cggcccagcg cgtgaaacgt
     2461 caccacgaac gggatgccga agcgtgtctt gagttcgagg gcggcaagcc ccgacatgaa
     2521 gaaattcgcg tggacgacgt catagcgacc gtgcgcccga cagaattccg ccacccccga
     2581 ggtgaactcc ggcatgccgg gcagcagggc ctccttgcgc agcgcctctt cggggccggc
     2641 acggacgtgc acgacccgca gaccgcttgt cagctcaacg acgtcggcca gcgtaggatt
     2701 gtcgcgacgc gtaaagatgt cgactgcgca acccatgctc tggacatgac gcgcgatgct
     2761 cgcaacatag acgttctgac cgccgctgtc gttgcctccc gcaatcgcaa gcggcgaagc
     2821 gtggtcacta atgatcgcga tctttctcgt catggcactc ctgcgtgaaa agtggcgtcg
     2881 aattgcgcgt gccccaacac ttggaaacgc gctcgcgacg aagcggggga agttcgaatg
     2941 ccgtggtcac agcaaacgat atgccacttg tctctgctgc tcgagtatga atagcgtcga
     3001 actgcagtgc ttcatagccg cttcgctcca tgcgacgcgc ttctacgccc agcaatgaat
     3061 cgactcgcac agcatttttt tcccggagtg ccgcgtaaga tttaccggcg ccgcaagggc
     3121 atcgctaccg gataccgcac
//
LOCUS       DASURG010000318         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1281746, whole genome shotgun sequence.
ACCESSION   DASURG010000318 DASURG010000000
VERSION     DASURG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281746"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFY80_05515"
     CDS             complement(<1..908)
                     /locus_tag="VFY80_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX6007398.1"
                     /translation="MSRVLRELSLDGTPYRYYSLRAAAAERGLAGLDRLPFSLRVLAE
                     NLLRHAGAADVDPEMLRALANGERGFDIPFRPARVLLQDLLGVPVMVDFAALREAIAR
                     AGGDPLAVNPKIPVDFVVDHSIIADVAGAADAEARNVRLEYARNRERFAFLAWCQSAF
                     CNFRVVPPDSGIVHQINVERLARVVWTAPHETATLAYPDTMVCNDSHSTMVNGIGVLG
                     WGVGGIEAEAAMLGRGLMLRVPEVIGVRVTGEMPAGATATDLVLTITEQLRRTGVVGK
                     FVEFFGPGLDALPVPDRATIANMAPE"
     gene            complement(996..1619)
                     /gene="plsY"
                     /locus_tag="VFY80_05520"
     CDS             complement(996..1619)
                     /gene="plsY"
                     /locus_tag="VFY80_05520"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX6007399.1"
                     /translation="MGITVFALIGYLLGSISFAVIVSRAFALPDPRTYGSGNPGATNV
                     LRTGRKAAAALTLLGDGAKGWLAVFLAQRYAAPETTDVAVACAAVAVVVGHMYPVFFR
                     FAGGKGVSTALGVLIGLNIYLALGTAATWIVMAVFFRISSLSALVSAIFAPFFTLILY
                     NAHHPYFSGVLVIALLLVWRHRSNIRNLLAGTEARIGRGAGRAGSGD"
     gene            1716..2072
                     /locus_tag="VFY80_05525"
     CDS             1716..2072
                     /locus_tag="VFY80_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX6007400.1"
                     /translation="MDIIFIDDFRLDILIGIYEWERRVPQTVQFNLEIAIPPKQGPRT
                     DRIGETIDYSKVVNRIEETLRTQHFLLLERLSEHVAQLVLTEFGAPWVKVSVNKIGAL
                     RNVKRLGVSIERGTKP"
     gene            complement(2162..3061)
                     /gene="xerD"
                     /locus_tag="VFY80_05530"
     CDS             complement(2162..3061)
                     /gene="xerD"
                     /locus_tag="VFY80_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX6007401.1"
                     /translation="MKASDSGLIDEFCDALWLEDGLARNTLESYRRDLVKFASWLAAK
                     HGRSLLGASRADLLGFLAHQVQRRAKATTASRELSSLKRFYRYAVRQAKIAADPTLNV
                     DSPKLPRPLPKSLTEQDVETLLAAPSVEEPLGLRDRAMLETLYASGLRVSELVSLRLS
                     QVSQDMGVVRVTGKGSKERLVPLGEEALAWIRRYIKEGRPALLRRRASEALFVTARGE
                     SMTRQAFWYLIKRYAARAGLAKPLSPHTLRHAFATHLLNHGADLRVVQLLLGHSDIST
                     TQIYTHVARERLKQLHARHHPRG"
     gene            complement(3033..>3470)
                     /locus_tag="VFY80_05535"
     CDS             complement(3033..>3470)
                     /locus_tag="VFY80_05535"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6007402.1"
                     /translation="LGIRTTPGTLKGIDYLPRGAATLAPLDRMAEKVCRQIARYLDDP
                     EFRFDLPFELSGTQFQCKVWREILRIRSGETLTYLAMARKLGTAPRPVGGACGANRIP
                     LVIPCHRVVGSAGIGGFMNARSGSPLEIKKWLLAHEGIGFRPD"
BASE COUNT          637 a         1163 c         1114 g          556 t
ORIGIN      
        1 tactccggcg ccatgttggc gatcgtcgcg cggtcgggca caggcagcgc gtcgagcccc
       61 gggccgaaga actcgacaaa ttttccgacg accccggtgc gacgcagctg ttcggtgatc
      121 gtcagcacga gatcggttgc cgtcgcaccc gccggcattt cgcccgtcac gcggacaccg
      181 atcacctccg gaacgcgcag catgagccct cggcccagca tcgcggcttc cgcttcgatg
      241 ccgcccaccc cccagccgag cacgccgatg ccgttcacca tcgtgctgtg gctgtcgttg
      301 cacaccatcg tgtcgggata cgcgagcgtt gccgtctcgt gcggcgccgt ccagacgacc
      361 cgcgcaagcc gttcgacgtt gatctggtgc acgatgccgc tgtccggcgg cacgacgcgg
      421 aaattgcaga acgcactctg gcaccacgcg agaaacgcga agcgctcgcg gttgcgcgca
      481 tactccagcc gcacgttgcg cgcttcggcg tccgcggcgc cggcgacgtc ggcgatgatg
      541 gaatgatcga ccacgaaatc caccggaatc ttcgggttca cggcgagcgg atcaccgccg
      601 gcacgcgcga tcgcctcgcg cagcgcggca aaatcgacca tcaccggcac gccgaggagg
      661 tcctgcagca gcacccgagc cggccgaaac gggatgtcga agccgcgctc gccgttcgcc
      721 agtgcgcgca gcatttcggg gtcgacatcg gcggcgcccg catggcgcag caggttttcc
      781 gcgagaacgc ggagcgagaa tggaagccgg tcaagccccg cgaggccgcg ctcggccgcc
      841 gcggcgcgca agctgtagta ccgataggga gtgccgtcga gagagagttc gcgcaagacg
      901 cgggacaatt ggggtcctct atacgtgatc cgttggaaat tatcgggcgg ccgcttgacg
      961 cgcttggctg cggggcacgc tccgggtatg agcgttcagt ccccggaccc cgccctgcct
     1021 gcgccgcggc caatccgcgc ttccgtgccc gcgagcaggt tgcggatgtt gctacggtga
     1081 cgccatacca gcagcagcgc gatcaccagc acgcccgaga agtacgggtg atgcgcgttg
     1141 tagaggatca gcgtgaaaaa cggcgcaaag atggcggaga ccagcgcgga aagcgaggag
     1201 atgcggaaaa acaccgccat cacgatccac gtcgccgcgg ttcccaaagc gagatagata
     1261 ttgaggccga tcaaaacgcc gagggccgtc gatacgccct tgccgcccgc gaagcggaag
     1321 aatacgggat acatgtggcc aaccaccacg gcgaccgccg cgcaggccac cgcaacatcc
     1381 gtcgtctcgg gcgcggcata gcgctgcgcg aggaaaaccg cgagccagcc cttcgcgccg
     1441 tcgcccagca gcgtaagcgc cgccgcagcc ttgcgccccg tgcgcagcac gttcgtcgca
     1501 ccgggattgc cggaaccgta ggtacgtgga tcgggaagcg cgaacgcgcg actcacgatc
     1561 accgcgaacg aaatcgaacc aaggagataa ccgatcagcg caaaaactgt gatgcccatg
     1621 ggatttcaca tagaatgtgc atccattcta tccgaaacgc atcgtgcgaa cgggttcccc
     1681 tcctccaatt ccctctgctt cctgattcct gatccatgga catcatcttc atcgacgact
     1741 tcaggctcga catcctgatc ggcatctacg agtgggaacg ccgcgtgccg cagacggttc
     1801 agttcaacct ggagatcgcg attccaccca agcagggccc gcgcaccgac cgcatcgggg
     1861 agaccatcga ttactcgaag gtcgtgaacc gcatcgagga gacgctgcgc acgcagcatt
     1921 ttctgctgct ggaacgcctg tccgagcacg tggcgcagct ggtgctcaca gagttcggcg
     1981 cgccctgggt gaaggtgagc gtgaacaaga tcggggcgct gcgcaacgtg aagcggctcg
     2041 gcgtctcgat cgagcgcggc acaaagccgt aaaccgtaag cggtgaatgg tgaagagtga
     2101 acggtagcca ctaagcgccg atctcgtcac catttgccgt tctccattca ccgttcaccg
     2161 tttaccctcg gggatggtgc cgcgcgtgca attgcttcag ccgctcgcgc gccacgtgcg
     2221 tgtagatctg cgtcgtcgaa atgtccgaat ggccgagcag cagctgcacc acgcgcaggt
     2281 cggcgccgtg gttcaggaga tgcgtcgcaa aggcgtggcg cagcgtatgc ggcgagagcg
     2341 gcttggcgag ccccgcccgc gccgcgtagc gcttgatgag gtaccagaac gcctggcgcg
     2401 tcatcgattc cccgcgcgcg gtcacgaaca gcgcctcgct tgcgcgccgg cggagcagcg
     2461 ccgggcgccc ttccttgatg tagcggcgaa tccacgcgag tgcctcctcg ccgagcggga
     2521 cgagccgttc cttggagccc ttgccggtca cgcgcacgac gcccatgtcc tgactcacct
     2581 ggctcagtct gagcgacacc agttcggaca cccgcaaccc gctcgcgtac aacgtttcga
     2641 gcatggcccg gtcgcgcagg cccagcggtt cttcgacgct gggtgcggcg agcagcgtct
     2701 cgacgtcctg ctcggtcaga ctcttgggca gcgggcgcgg cagcttgggt gaatcgacgt
     2761 tgagcgtcgg atcggcggcg atcttcgcct gccgcacggc gtagcgataa aaccgcttca
     2821 ggctcgagag ttcgcggctc gcggtcgtcg ccttggcgcg gcgctgaacc tgatgcgcga
     2881 gaaatccgag cagatcggcg cgacttgcgc cgaggaggga ccggccgtgc ttcgccgcga
     2941 gccacgaggc gaacttgacg agatcgcgcc gatagctttc cagcgtgttg cgggcaagcc
     3001 cgtcctccag ccacagcgcg tcgcagaact cgtcaatcag gccggaatcc gatgccttca
     3061 tgcgccagca gccacttctt gatttcgagc ggcgatccgc tcctcgcgtt catgaatccg
     3121 cctattccgg ccgatcccac gacgcgatgg cacggtatca cgagcgggat gcgattcgcg
     3181 ccgcacgcgc cgcccaccgg acgcggcgcc gtcccgagct tgcgcgccat cgccaagtac
     3241 gtgagcgtct cgccgctgcg gatgcggaga atttcgcgcc acaccttgca ctggaattgc
     3301 gtgccgctca gctcgaacgg caggtcgaag cgaaactcgg gatcgtccag gtagcgcgcg
     3361 atctgccggc acaccttctc cgccatgcgg tcgagcggcg cgagcgtcgc cgcgccccgc
     3421 ggaagatagt cgatgccttt caatgtgccg ggcgtggtgc gaatgcccag
//
LOCUS       DASURG010000319         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_872549, whole genome shotgun sequence.
ACCESSION   DASURG010000319 DASURG010000000
VERSION     DASURG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872549"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFY80_05540"
     CDS             complement(<1..678)
                     /locus_tag="VFY80_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6007403.1"
                     /translation="MQRSILGCLLALLALMVAPVAAQEKVNLSIATGGTGGVYYPLGG
                     GMANVLSKYVPGMQATAEVTGGSVDNLKLIGTGKPYIALAMADASQDAYQGQDKFKGS
                     KVPVRTLAILYTNHMHVVTVEGTGINTIKDLKGKRVSTGSGGSATEVMAFRVIEAAGL
                     DKDRDMRRERLGVAESVAALKDRKIDAFFWVGGLPTAAVTDLANSPGVKIKMIDHAEL
                     VPAMNKKY"
     gene            complement(781..1206)
                     /locus_tag="VFY80_05545"
     CDS             complement(781..1206)
                     /locus_tag="VFY80_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6007404.1"
                     /translation="MTPPLFHLAFPVTDLAKARAFYGGLLGCPEGRSSDTWVDFDFYG
                     HQIVAHLAPQEAGHRHTSAVDGEDVPVRHFGVILTMPAWQALADKLIAADVNFVIEPH
                     VRFKGEVGEQATMFFLDPCGNALEFKAFKDVSQVFARAH"
     gene            1280..>2199
                     /locus_tag="VFY80_05550"
     CDS             1280..>2199
                     /locus_tag="VFY80_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6007405.1"
                     /translation="MPKIQSVSVCVARVPLDVVTSFATRTVSARDYCLVKVRSTDGVE
                     GIGFCYVGSAGGNIAKVAVEELFARKLIGQDSLRIEGLWQDMYQDSLLQGRSGSVMRA
                     ISILDIALWDLNARTARLPLHQYLGAFVMNEVPAYASGGYYVEGKTPRKLGDEMASYV
                     KAGFKAVKMKVGRLSPRDEEARVKAAREAVGPDIVLTLDANNAWSDVPTALTYMKRYE
                     PYDPYWIEEPFGPDDIDGHAKLAELTPVTVATGEIAYGRWYHKELLDKRGAEILQTDA
                     CVCGGITEWKRIAATAASYGVTMCPHWFHDV"
BASE COUNT          414 a          692 c          697 g          396 t
ORIGIN      
        1 gtacttcttg ttcatggccg gcaccagctc ggcgtggtcg atcatcttga tcttcacgcc
       61 gggtgagttg gcgaggtcgg tgaccgccgc ggtcggcagg ccgcccaccc agaagaaagc
      121 gtcgatcttg cgatccttca gcgccgcgac cgattcggcc acgccgaggc gctcgcgccg
      181 catgtcgcgg tccttgtcga ggccggccgc ctcgatcacg cgaaaggcca tgacctcggt
      241 ggcgctgccg cccgaacccg tggacacgcg cttgcccttc agatccttga tggtattgat
      301 gccggtacct tccaccgtga cgacgtgcat gtggttggta tagaggatcg ccagcgtgcg
      361 cacggggact ttcgagcctt tgaacttgtc ctgcccctga taggcatcct ggctcgcatc
      421 cgccatcgca agggcgatat acggcttccc ggtgccgatc aatttgaggt tgtccaccga
      481 cccgccggtg acttccgcgg tcgcctgcat tccgggcacg tacttggaaa gcacattggc
      541 catgccaccg ccgagcgggt agtagacgcc gcccgttccg ccggtggcga tcgagaggtt
      601 caccttttcc tgcgccgcga ccggggcaac catcaaggca agcaatgcca gcaggcatcc
      661 aagaatcgac ctttgcatca tgttctccta ctgctccttt gtttcggtta gtaagtgaga
      721 ggaaaaaaga gtttcataac tcgtggcgcg gtcgcgcacg gtcaaacggc tcaatttcgg
      781 ctaatgggcc ctggcgaaga cctgggagac atccttgaac gccttgaatt cgagcgcgtt
      841 gccgcacgga tcgagaaaaa acatcgtcgc ctgctcgccc acctcgccct tgaagcgcac
      901 atgcggctcg atcacgaaat tcacgtccgc cgcgatgagc ttatcggcga gtgcctgcca
      961 tgccggcatg gtgaggatca cgccgaagtg gcgcacgggg acgtcttcgc cgtcgaccgc
     1021 gctggtgtgg cggtgtcccg cctcctgagg cgccaggtgg gcgacgatct gatggccata
     1081 gaaatcgaaa tcgacccagg tgtccgaact gcggccttcg gggcagccga ggagcccgcc
     1141 gtagaaggcg cgcgccttcg cgaggtccgt caccgggaat gccagatgaa aaagcggcgg
     1201 cgtcatgtct ggaaccatgc cgtcatgcaa aaaagggtgg cattatacgg attttcccgc
     1261 attacgaaag accgtcggca tgcccaaaat ccaatccgtt tcagtctgcg tggcgcgcgt
     1321 gccgctcgac gtggtcacct ccttcgcgac acgcaccgtg agcgcccgtg attactgcct
     1381 ggtcaaggta cgctccaccg acggggtgga aggcataggg ttctgctatg tcggcagcgc
     1441 cggcggcaac atcgccaagg ttgccgtgga agaactcttc gcgcgcaaac tgatcggcca
     1501 ggattcgctg cggatcgagg ggctgtggca ggacatgtat caggattcgc tcctgcaggg
     1561 ccgctcgggg agcgtcatgc gcgcgatcag cattctcgac atcgcgctgt gggacttgaa
     1621 cgcccgcacg gcgcggctgc cgctgcacca gtatctcggc gcgttcgtca tgaacgaagt
     1681 gccggcatac gcgagcggcg gctactacgt cgaaggcaag acgccgagga agctcggcga
     1741 cgagatggcc tcctacgtca aggccggatt caaggcggtg aagatgaaag tggggcggct
     1801 ttcgccacgc gacgaggaag cgcgcgtcaa ggcggcgcgc gaggcggtgg ggccggacat
     1861 cgtgctgacc ctcgacgcga acaacgcgtg gtcggacgtg ccgaccgcgc tcacctacat
     1921 gaagcgctac gagccctacg atccgtactg gatcgaagaa ccgttcggcc ccgacgacat
     1981 cgacggccac gccaagctcg cggagctgac acccgtcacc gtggcgaccg gcgaaatcgc
     2041 ctatggccgc tggtatcaca aggaactgct cgacaagcgc ggcgcggaga tccttcagac
     2101 cgatgcgtgc gtgtgcggcg gcatcaccga atggaagcgc atcgccgcga ccgccgcgtc
     2161 ctacggcgtc accatgtgcc cgcactggtt ccacgacgt
//
LOCUS       DASURG010000320         4917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_567362, whole genome shotgun sequence.
ACCESSION   DASURG010000320 DASURG010000000
VERSION     DASURG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567362"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VFY80_05555"
     CDS             <1..134
                     /locus_tag="VFY80_05555"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6007406.1"
                     /translation="IANGILTTDTEEQALARMTEKGADCALAAIEMANLLKKLDEQD"
     gene            121..546
                     /gene="nusB"
                     /locus_tag="VFY80_05560"
     CDS             121..546
                     /gene="nusB"
                     /locus_tag="VFY80_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX6007407.1"
                     /translation="MSKTSRRRAREFALQGLYQWQLAKSEPDTIAQQLAEAKAFDKLD
                     TDYFRSLLHGTVRDAVELERDISPCLDRPFAALSPVERGILFLAAYEFRHHLEVPYRV
                     VINEAVELAKEYGGTDGYKFVNGVLDRLAARLRAAEAKP"
     gene            543..806
                     /locus_tag="VFY80_05565"
     CDS             543..806
                     /locus_tag="VFY80_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007408.1"
                     /translation="MTGKSMDERLKKRMYAFYFAGVVNLVLGLYVLTAGAAHMGEGTA
                     LLVALFFLGFAAVDFYFPRAMKKKWLEEQSRMRAAGEPRDSAK"
     gene            817..1785
                     /gene="thiL"
                     /locus_tag="VFY80_05570"
     CDS             817..1785
                     /gene="thiL"
                     /locus_tag="VFY80_05570"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX6007409.1"
                     /translation="MLSEFELIRRYFTHPAPHTRLGVGDDAAVIRPRNGMELVVTVDM
                     LVSGTHFFADAEPERLGHKALAVNLSDIAAMGAVARWTTLALALPAVEPRWLRKFASG
                     FMRLARRHDVALIGGDTTRGPLAICVQVLGEVPAGQALLRSGARVGDDVWVSGRIGDA
                     ALAIAARKGRVKLATREWSAVRRRLDAPMPRLALGAALRGIATSAIDISDGLCADLGH
                     ICERSKVAAVIEFERLPVSAAARKRLDSRVVRAAVLTGGDDYELCFTARPGRREAVCA
                     AAERAEVPVTRVGRIERVLKRGARVTIADRRGRPMALTHGGYEHFR"
     gene            1763..2275
                     /locus_tag="VFY80_05575"
     CDS             1763..2275
                     /locus_tag="VFY80_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX6007410.1"
                     /translation="MDTSISADRAGRASWRFVLQHPAHLLAFGFGAGLLPAAPGTFGT
                     LLAFPAFWLLSGWISDGALFAVIAVMLLAGIGLCGSTGRALGQADHGGIVWDEIAAFM
                     LVLFFTPAGTAWQGFAFVLFRVFDIVKPPPIRYYERKLPGGLGVMFDDLLAAFYTLLV
                     LAITRTWIGS"
     gene            2272..2766
                     /locus_tag="VFY80_05580"
     CDS             2272..2766
                     /locus_tag="VFY80_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX6007411.1"
                     /translation="MNDKALYELAEQVGYALRDLKLMLATAESCTGGGVGEAITRVPD
                     SSHWFERGFITYTYISKREMLGVESDTLGRYGAVSEPTVREMVAGALERSHAQVAVAV
                     SGTAGPGGGTAEKPVGTVCFAWGIKGGVVASETQHFAGDRQSVRMQSVAHALKGVLRL
                     LESR"
     gene            2912..3955
                     /gene="recA"
                     /locus_tag="VFY80_05585"
     CDS             2912..3955
                     /gene="recA"
                     /locus_tag="VFY80_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX6007412.1"
                     /translation="MDENRTKALAAALSQIEKQFGKGSIMRLGESDVARDIQVVSTGS
                     LGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLSVIAQMQRQGGTAAFIDAEHALDPS
                     YAKKLGVNVDDLLVSQPDSGEQALEIADMLVRSGSVDVVVIDSVAALVPKAEIEGEMG
                     EPQMGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRIDIRRVGSIKKGDEVIGSETRAKVVKNKVAPPFRNADFDILYGEGISREGEIIE
                     LGVAHGIIDKSGAWYTYGKERIGQGKDNTRDYLKEHPDLAREIEMKVLAAVGIGVAEP
                     KVEAKPEPRKAVG"
     gene            3900..4397
                     /gene="recX"
                     /locus_tag="VFY80_05590"
     CDS             3900..4397
                     /gene="recX"
                     /locus_tag="VFY80_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEX6007413.1"
                     /translation="MSQSPKSKRSPSPEKLSDSALRSHALRLLARREHSRRELQRKLA
                     ALAAEPDEVERILDELTARGWLAERRVAEQLIHARRGRFGSGRIRQELLAKGVAEEVV
                     AAVLPGLKESELDAARTVWRRKFKVPPSSAKERARQVRFMQGRGFAMDTIMKVIRPGG
                     ADDES"
     gene            4387..4737
                     /locus_tag="VFY80_05595"
     CDS             4387..4737
                     /locus_tag="VFY80_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007414.1"
                     /translation="MNRRRVAFAVLAALGLLAGCDYLPFGYTPIRDIVASPGKFESQE
                     VKIRGKVTESMKLPILGQSFIVRDDTGEIAVMTNGTLPAPNADVALTGIVKSAAIIGG
                     QSIGLRVEETKRLR"
     gene            4777..>4917
                     /locus_tag="VFY80_05600"
     CDS             4777..>4917
                     /locus_tag="VFY80_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX6007415.1"
                     /translation="MKSSDIRKAFLDYFARQKHAVVPSSPLVPANDPTLLFTNSGMVQ
                     FKD"
BASE COUNT          869 a         1484 c         1701 g          863 t
ORIGIN      
        1 cgattgcaaa cggtattctg acgaccgata cggaagaaca ggcactcgcg cgcatgacgg
       61 agaagggcgc ggactgcgcg ctcgccgcga tcgaaatggc gaatctgctg aagaaactcg
      121 atgagcaaga ctagccgccg gcgcgcgcgc gagtttgcgc tgcagggcct ttatcagtgg
      181 cagctcgcga aatccgaacc ggacacaatc gcgcagcaac tcgcggaggc gaaagccttc
      241 gacaagctcg atacagacta tttccggtcg cttctgcacg gcaccgtgcg cgatgcggtg
      301 gagctcgagc gcgacatttc accctgcctc gaccggccct ttgcggcgct gtccccggtg
      361 gagcggggca tcctctttct cgccgcctac gagttcaggc atcacctcga agtgccgtac
      421 cgcgtcgtga tcaatgaagc ggtggagctg gcaaaggaat acggcggcac cgacggctac
      481 aagttcgtga acggcgtgct cgaccgcctc gccgcgcgcc tgcgcgcagc ggaagcgaaa
      541 ccgtgacggg gaagagcatg gatgagcggc tcaagaagcg gatgtacgcg ttctacttcg
      601 ccggtgtggt caacctcgtg ctcggacttt acgtcctcac ggccggtgcc gcgcacatgg
      661 gcgaggggac cgcgctcctg gtcgcgctgt ttttcctggg ctttgccgcc gtggacttct
      721 atttcccgcg cgcgatgaaa aaaaagtggc tcgaggagca gtcccggatg agggcggcgg
      781 gcgagccgcg cgacagcgcg aaatagcggg ccacgcgtgc tctccgaatt cgaactgatc
      841 cggcgctact tcacccatcc ggctccgcac acccggctcg gcgtcgggga cgacgcggct
      901 gtcattcgtc cgcggaacgg catggagctc gtggtgacgg tcgacatgct ggtttcgggc
      961 acgcacttct ttgcggatgc cgagcccgag cggctggggc acaaggcgct cgccgtcaat
     1021 ctttccgaca tcgctgccat gggggcggta gcgcgctgga cgacgctcgc gctcgcgctg
     1081 cccgccgtcg agccgcggtg gctgcggaaa ttcgcctccg gcttcatgcg gcttgcgcgg
     1141 cgccacgacg ttgcgctcat cggcggcgat acgacgcgcg gtccgctcgc gatctgcgtc
     1201 caggtcctgg gcgaagtgcc ggccggacag gcgctgcttc gcagcggcgc gcgggttggc
     1261 gacgatgtct gggtgtcggg gcgcatcggt gatgcggcgc tggctatcgc tgcccgcaag
     1321 ggacgggtga agctcgcgac acgcgaatgg agtgccgtgc ggcgccggct cgatgctccg
     1381 atgccgcgtc tcgccctcgg cgctgcattg cgcgggatcg caactagcgc catcgacatc
     1441 tcggacggcc tgtgtgcgga tcttggtcac atctgcgagc gctcgaaagt tgcagcggtg
     1501 atcgagttcg aacggctgcc ggtttcggcg gcagcaagaa agcggctcga ttcgcgcgtg
     1561 gtgcgcgcgg cggtgcttac gggcggcgat gattatgagt tgtgtttcac cgcgcgtccc
     1621 ggacgcaggg aggccgtgtg cgccgctgcc gagcgggctg aggtaccggt cacccgggtg
     1681 gggcggatcg agcgtgtcct gaagcggggt gcacgcgtga ccatcgcgga tcgaagggga
     1741 cggccgatgg cgctgacgca cggtggatac gagcatttcc gctgaccgcg ccggccgggc
     1801 gtcgtggcgc ttcgtcctgc agcatccggc gcatctgctt gcattcggct tcggggccgg
     1861 cctgctcccc gctgcgcccg gcacgttcgg tacgctcctc gcatttccgg cattctggct
     1921 gctgtcggga tggatttcgg acggcgcgct gttcgccgtg atcgctgtga tgctcctggc
     1981 gggaatcggg ctgtgcggta gcaccggccg cgcgctcggt caggccgatc acggcggcat
     2041 cgtatgggac gaaatcgcgg cgttcatgct ggtgctgttc ttcacccctg ccggcaccgc
     2101 gtggcagggt ttcgcattcg tcctgtttcg agtcttcgac atagtgaagc cgccgccgat
     2161 acgctattac gagcggaagc ttccaggcgg gcttggcgtc atgttcgacg acctgctcgc
     2221 agccttctac actcttctcg tgctcgccat cacaaggaca tggatcggat catgaatgac
     2281 aaggcgttgt atgaactcgc ggaacaggtc ggctacgccc tgagggacct gaagctcatg
     2341 ctcgcgacgg cggagtcctg cacgggaggc ggcgtaggcg aagccattac gcgcgtcccc
     2401 gacagctcac actggttcga acgcggcttc attacgtata cctacatttc caagcgggaa
     2461 atgctcggcg tcgaatccga tacgctcggg cgctacgggg cggtctccga gccgacggtg
     2521 cgcgaaatgg tggcgggtgc gcttgagcgc agccatgcgc aagtcgccgt ggcagtgagc
     2581 ggaaccgcag gtccgggcgg cggcacggcg gagaagccgg tcggcacggt ttgcttcgcg
     2641 tggggcatca agggcggcgt ggtcgcgagc gagacacaac attttgcggg ggatcggcag
     2701 tcggtgcgca tgcagtcggt cgcgcatgcg ctgaagggcg tgctgcgcct gctggaaagc
     2761 agatgaaccc ggcgaacgca gtcatccatt gctgctgcga ggcgttaagc cttgcagacg
     2821 caatggagcg tgctcagcgt taaacgaagc gcttcattat gtcgaccggt ggaggtgtga
     2881 cataatctac agcttgtaca cggggggaaa gatggacgaa aacaggacca aggcgctggc
     2941 cgcggcgcta tcgcagatcg agaagcaatt cggtaagggt tcgatcatgc gcctcggcga
     3001 atcggacgtg gcccgggata tccaggtggt ctcgaccggt tccctcggac tcgatatcgc
     3061 gctcggcatc ggcgggcttc cgcgcgggcg cgtggtggag atctacggac ccgagtcctc
     3121 gggcaagacc accctcacgc tgtcggtgat cgcccagatg cagcggcagg gcggcaccgc
     3181 cgccttcatc gacgccgagc acgcgctcga tccgtcttat gccaagaagc tcggggttaa
     3241 tgtcgatgac ctcctcgtgt cgcagcccga ttccggcgag caggcgctcg agatcgccga
     3301 catgctggtg cgctcgggct cggtggatgt ggtcgtgatc gactcggtgg cggcgctcgt
     3361 gcccaaggcg gaaatcgagg gcgagatggg cgagccgcag atggggctgc aggcgcggct
     3421 gatgtcgcag gcgctccgga agctcaccgc gaacatcaag cgctccaata cgctcgtcat
     3481 cttcatcaac cagatccgca tgaagatcgg cgtcatgttc ggcaatcccg agacgacgac
     3541 cggcgggaac gcgctcaagt tctacgcctc ggtgcggatc gacatccgcc gcgtcggatc
     3601 gatcaagaag ggtgacgagg tgatcggctc cgaaacccgg gccaaggtgg tgaagaacaa
     3661 ggtggcccct ccgttccgca acgccgattt cgacatcctc tacggcgagg gcatctcgcg
     3721 tgaaggcgag atcatcgagc tcggtgtcgc gcacggcatc atcgacaagt ccggcgcgtg
     3781 gtacacctac ggcaaggagc gcatcgggca gggcaaggac aacacgcgtg attacctcaa
     3841 ggaacatccc gacctcgcgc gtgaaatcga gatgaaggtg ctcgccgcgg tggggatcgg
     3901 tgtcgcagag cccaaagtcg aagcgaagcc cgagccccga aaagctgtcg gatagcgcgc
     3961 ttcgatcgca cgcgctcagg ctgctggcgc ggcgcgagca ctcgcggcgg gagctgcagc
     4021 gcaagctcgc agccctggcg gcggagccgg acgaggtcga gcgtattctc gatgagctga
     4081 ccgcgcgcgg ctggcttgcg gagcgccgcg tcgccgagca actgatccat gcccggcgcg
     4141 ggcgtttcgg cagcggccgc atccggcagg aactgcttgc caagggcgtg gccgaagaag
     4201 tggtcgcagc cgtgctcccc ggtttgaaag agagcgagtt ggacgcagcg cgcacggtgt
     4261 ggcggcgcaa gttcaaggtg ccgccctcga gcgcgaaaga acgtgcgcgg caggtgagat
     4321 tcatgcaggg ccgcggtttt gccatggata ccatcatgaa agtaattcga cccggcgggg
     4381 cggacgatga atcgtaggcg cgttgcgttc gccgtgctgg cggcgctcgg actgctggcg
     4441 gggtgcgact atctcccatt cggctacacg ccgatcaggg atatcgtcgc gtcacccggc
     4501 aagttcgaga gccaggaagt gaagatcagg ggcaaggtga ccgaatcgat gaagcttccg
     4561 atcctggggc agagcttcat cgtgcgggac gacacgggcg agatcgccgt catgacgaac
     4621 ggcacgctgc ctgcgccgaa tgccgacgtc gccttgaccg gcatcgtgaa gagcgccgca
     4681 atcatcggcg gacagtcgat cggtttgaga gttgaggaaa cgaaacgcct gcgctagttt
     4741 gatcggctcg gctcaggcat ttcttacagg gcattcatga aaagcagcga catacggaaa
     4801 gcgtttctcg attacttcgc gcgccagaag catgcggtcg tgccctcgag cccgctcgtt
     4861 ccggccaacg acccgacgct gctcttcacg aattccggca tggtgcaatt caaagac
//
LOCUS       DASURG010000321         4647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_873218, whole genome shotgun sequence.
ACCESSION   DASURG010000321 DASURG010000000
VERSION     DASURG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873218"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1786)
                     /locus_tag="VFY80_05605"
     CDS             complement(68..1786)
                     /locus_tag="VFY80_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6007416.1"
                     /translation="MTARATPSQLAVALLIAAFLVIFLVVPVLTVVYVAFADGAGGVT
                     LSHFASFFHISLMRESFWNSLYVAFWSVVFASLIAVPLAYFTVRFEFRGAVLIQTLGV
                     LPLIMPPFVGAVAMQLIFGRSGTVNLLLNDWFDISLPIMEGLNGVIFVEAIHYFPFIL
                     MNLVVALNNIDSSMEESAQNLGSSGLRLFRRIVFPLAMPGFIAGAALVFIKVFDDLGT
                     PLVLNQTNMLAPQAYLRITTIGIEDPIGYVASVIMVVFSLMALWASTWVMRDKEYATL
                     QRGYGGLARRRLTGAHSALAYAWIVLVLLVVLAPHVGILLLSMARVWSFSVVPESYTL
                     EHYATVFQDSPGMIWNTLLYCGVAAVLDVVIATAIAYILLRTRIPGRKALDFTVTMAL
                     AIPGVVLAIGYLRTFRDIEIPFTGDLLTTSWMLLIIAYAVRRLPYALRSCVAALQQMH
                     VSLEEAAENLGATKGRTIRRIVVPLMAGGIAAGLIISFMTAAVELSATLMLVTAQHDA
                     PMSYGIYLYMQSAAGRGPGAALGVLAVLIVAAGTYLSHRVIASRAAREKRDAIITAPE
                     IQTAGA"
     gene            complement(2002..3288)
                     /locus_tag="VFY80_05610"
     CDS             complement(2002..3288)
                     /locus_tag="VFY80_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6007417.1"
                     /translation="MSVSAFVVNAQQQLTIITSFPKDLTEVYKKAFETRNPGVKVEVL
                     NRGTSAAIAFVREARAGSRPDVFWASAPDAFEVLAKENLLEKFSGANPAVPAKIGHYP
                     INDPEGYYYGQALAGYGLMWNTRYLKANKVPPPREWADLVKREYFGHVAISAPSRSGT
                     THLTIETILQGEGWDKGWQQILQFSGNCAAITERSFGVPDGVNNGQFGIGIVIDFFGL
                     SAKASGFPVEFVYPSVTAIVPANIGLVAGGRNSDLGKKFIAFALSEEGQQLLLEPRIS
                     RLPVLPAVYKKAPAGYPNPYGGKIQAKVKFDSDLSRSRYYLVNSLYDHTITFRHKELV
                     AATRAIHEAAAKLSKKSNAQAKKLLDEAREYAYKPLVSAEKAADKDFLALYSTTKRDA
                     LRTKEVTALEEYWGREARKNYARAEELAKQAAALVK"
     gene            3523..>4647
                     /gene="htpG"
                     /locus_tag="VFY80_05615"
     CDS             3523..>4647
                     /gene="htpG"
                     /locus_tag="VFY80_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX6007418.1"
                     /translation="MPTRETLGFQAEVKQLLNLMIHSLYSNKEIFLRELISNASDACD
                     KLRFEALTDKALFEDDPELRIRVSFDPKGRTITVSDNGIGMSRQEVIEHIGTIAKSGT
                     REFFQSLTGDQAKDAHLIGQFGVGFYSSFIVADKVTLETRRAGLGKEHGVRWESDGSG
                     EYTVETVERATRGTDVTLHLREGNDDLLSGFRIRDIIRKYSDHITLPIVMKEEEWDKD
                     NRLYRTTGKDETVNQASALWARPKSEITEEQYHEFYKHVAHDFEPPLAYTHNKVEGTK
                     EYTQLLYIPARAPFDIWDREHRRGIKLYVRRVFIMDDAEHLMPGYMRFVRGVIDSNDL
                     PLNVSREILQESKDVEAIRSGCARRVLALLEDIATNQPEKY"
BASE COUNT          917 a         1419 c         1526 g          785 t
ORIGIN      
        1 acttttgttt cgccgtatga cagcgagacg ccacgcactt ccactttgac cttgcgcaac
       61 ccttccatca cgcccccgcc gtctggattt ccggcgccgt gatgatcgcg tcgcgctttt
      121 cgcgtgcggc gcggcttgcg atgacgcggt gcgacaggta ggtgccggct gcgacgatca
      181 gcactgcgag cacgccgagg gcggcgccgg gcccgcggcc cgccgcgctc tgcatgtaga
      241 gatagatgcc gtaggacatg ggcgcatcgt gctgcgccgt gaccagcatg agcgtcgccg
      301 aaagctccac cgccgccgtc atgaagctga tgatgaggcc cgcggcgatg ccgcctgcca
      361 tgagcggtac gacgatccgg cggatggtgc ggcccttggt cgcgccgagg ttttcagccg
      421 cctcctccag cgacacgtgc atctgctgga gcgccgccac gcaggatcga agcgcgtagg
      481 gcaagcggcg cacggcgtag gcgatgatga gcagcatcca ggaagtcgtc agcagatcgc
      541 cggtgaatgg aatctcgatg tcgcggaaag tgcggaggta tccgatggcg agcaccacgc
      601 ccggtatggc gagcgccatg gtgacggtga agtcgagcgc cttgcggccc ggtatgcgcg
      661 tgcgcagcag gatgtacgcg atcgcggttg cgatcaccac gtcgagcact gccgccacgc
      721 cacagtagag cagcgtgttc cagatcatcc ccggcgaatc ctggaacacg gttgcatagt
      781 gctccagcgt gtagctttcc ggaacgacgc tgaagctcca caccctggcc atggagagga
      841 gcaggatgcc gacgtggggg gcgaggacga cgagcagcac gagaacgatc caggcatagg
      901 cgagcgcgga atgcgcaccg gtgaggcggc gccttgcgag tccaccgtat ccgcgctgca
      961 gggtcgcgta ctccttgtcg cgcatgaccc acgtcgacgc ccacagcgcc atcagggaaa
     1021 aaaccaccat gatgacgctc gccacgtagc cgatcgggtc ttcgatgccg atcgtcgtga
     1081 tacgcagata ggcctgcggc gcgagcatgt tggtctggtt cagcacgagc ggcgtgccga
     1141 ggtcgtcgaa gaccttgatg aacactagcg ctgcgccggc aatgaagccg ggcatggcga
     1201 gcgggaagac aatgcggcgg aaaagacgca agccggacga gccgagattc tgggccgatt
     1261 cttccatcga gctgtcgatg ttattgagcg cgacgacgag gttcatcagg atgaacggga
     1321 agtagtggat cgcctcgacg aagatcacgc cgttcaagcc ctccatgatc gggagcgaga
     1381 tgtcgaacca gtcgttgagg agcaggttca ccgtgccgct ccgcccgaag atgagttgca
     1441 tggcgacggc gccgacgaac ggcggcatga tgagcgggag cacgccgagc gtctggatca
     1501 gcactgcgcc gcgaaattcg aagcgcaccg tgaagtaggc gagcggcacg gcaatgagtg
     1561 acgcgaagac gacgctccag aacgcgacgt agaggctgtt ccagaacgat tcccgcatga
     1621 gagagatgtg gaagaacgag gcgaagtgcg aaagcgtgac gccgcccgcg ccgtcggcga
     1681 aggcgacgta gacgaccgtg agcaccggca cgacgagaaa gatgacgaga aacgccgcga
     1741 tgagcagcgc gactgcgagc tgcgacggcg tcgcgcgagc cgtcatgcgg agaaaaggaa
     1801 ggttcgggtc gttgcagacg acggcgcgtc aggcgatcgt ctccccggct caaccgatcg
     1861 tctctccggc ggaagccggg gtccagaaat gcactggatt ccggttttca ccggaatgac
     1921 gagcaacctg tccggaacga cgagcagcct gtccggagtt acgcgccgtc gtgcgctggg
     1981 atgaccgatg cgccgcatcg gtcacttcac cagcgcggca gcctgcttcg cgagctcttc
     2041 ggcacgcgca tagttcttgc gcgcttcgcg cccccagtat tcctccagcg cggtcacttc
     2101 cttcgtgcgc agcgcgtcgc gcttggtcgt gctgtagagg gcgaggaaat ccttgtctgc
     2161 ggccttttcc gcactgacga gcggcttgta ggcgtattct cgcgcctcat ccaacagctt
     2221 cttcgcctgg gcgttcgact tcttcgagag cttggccgcc gcctcgtgta tcgcgcgcgt
     2281 cgccgccacg agctccttat gacggaacgt aatcgtgtgg tcgtagaggc tattcacgag
     2341 gtaataacgg gagcgcgaga ggtccgaatc gaacttgacc tttgcctgga tcttgccgcc
     2401 gtagggattg ggatagccgg cgggcgcttt cttgtagacc gcaggcagca ccggcaagcg
     2461 gctgatgcgt ggctcgagaa gcaactgctg gccttcctcg gaaagcgcga acgcaatgaa
     2521 cttcttcccg agatcgctgt tgcgcccgcc ggcgacgagc ccaatgttgg cggggacgat
     2581 ggcggtgacc gacggataga cgaactcgac gggaaagccg gacgctttcg cggaaagtcc
     2641 gaagaaatcg atcacgatgc cgatgccgaa ctgcccgttg ttgacgccat cgggtacgcc
     2701 gaaactgcgc tcggtgatgg cggcgcagtt tcccgaaaac tgcaggatct gctgccagcc
     2761 cttgtcccag ccctcaccct gcaggatggt ttcgatggtg aggtgcgtcg ttccggagcg
     2821 ggacggcgcg gagatcgcga catgcccgaa atactcgcgc ttcacgaggt ccgcccactc
     2881 gcggggcggc ggcaccttgt tcgccttgag gtagcgcgtg ttccacatga ggccgtatcc
     2941 tgcgagcgcc tgcccgtagt aatagccttc agggtcgttg atggggtaat ggccgatctt
     3001 cgccggcacg gccggattcg cgccgctgaa cttctcgagc aggttctcct tggccagcac
     3061 ctcgaacgca tccggtgcgg aagcccagaa gacgtcgggg cggctccccg cacgcgcctc
     3121 gcgcacgaag gcgatagccg ccgaggtccc gcggttcagc acctcgacct tgacacccgg
     3181 gttgcgcgtc tcgaaagcct tcttgtagac ctcggtcagg tccttgggaa acgatgtgat
     3241 gatggtgagc tgctgctggg cgttgacgac gaaagcggat acgctcaacg cggcagcaag
     3301 cgcgacacgg ggcaagatcc ggaatgtgga cacgatccct cctgttgtaa gtgttatgcg
     3361 gggtactgcg gccatgttac cgttctccgc gcccggccgc caagcgcgcc gctcgtccac
     3421 ggcttgaaat tcggcggcgg cggcccaatg atttcgaggt cctgaaaatg cggcgatacc
     3481 cttttgccgc catagagaac caaaggagtg ccgatgggcg acatgccaac ccgcgagacg
     3541 ctcggcttcc aggccgaagt gaagcagctg ctcaacctca tgatccactc cttgtacagc
     3601 aacaaggaga ttttcctgcg cgagctgatc tccaatgcat cggacgcgtg cgacaagctg
     3661 cgattcgagg cgctgaccga caaggcgctc ttcgaggatg acccggagct cagaatccga
     3721 gtgagcttcg acccgaaagg ccggacgatc accgtgtcgg acaacggaat cggcatgtcg
     3781 cgccaggaag tgatcgagca catcggcacc atcgccaagt cgggcacccg cgagttcttc
     3841 cagagcctca ccggagacca ggcgaaggac gcgcacctga tcgggcagtt cggcgtgggg
     3901 ttctactctt ccttcatcgt cgccgacaag gtgacgctgg agacgcgccg tgccggcctt
     3961 gggaaggaac acggcgtgcg ctgggagtcg gacgggagcg gtgaatacac cgtcgagacg
     4021 gttgagaggg cgacgcgcgg cacggacgtg acgctgcatc tgcgggaggg caacgacgac
     4081 ctgctgagcg gattccggat ccgcgacatc attcgcaagt actccgatca catcacgctc
     4141 ccgatcgtga tgaaagagga ggagtgggac aaggacaatc gcctctacag gacgaccggc
     4201 aaggacgaga ccgtcaacca ggcgagcgcg ctgtgggccc ggcccaagtc cgagattacg
     4261 gaggagcagt atcacgagtt ctacaaacac gtcgcccatg acttcgagcc gcccctcgcc
     4321 tatacccaca acaaggtcga gggcaccaag gagtacacgc agctccttta catcccggcg
     4381 cgcgcgccgt tcgatatctg ggaccgcgag catcgccgcg gcatcaagct ctacgtgcga
     4441 cgcgtattta tcatggacga cgccgagcac ctgatgcccg gctacatgcg cttcgtgcgc
     4501 ggcgtgatcg actcgaacga tctgccgctc aacgtctcgc gcgagatcct gcaggagtcg
     4561 aaggacgtcg aggcgatccg ctcgggttgc gcgcggcgcg tgctcgcgct cctcgaggac
     4621 atcgcgacga accagcccga gaagtac
//
LOCUS       DASURG010000322         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_670067, whole genome shotgun sequence.
ACCESSION   DASURG010000322 DASURG010000000
VERSION     DASURG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670067"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFY80_05620"
     CDS             complement(<1..395)
                     /locus_tag="VFY80_05620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6007419.1"
                     /translation="MPPPPVAARELAREQRFSRIWTQELNRVFADVAPYYDSANHVAS
                     LGMWNHFLRSFLDTIDLRPGARVLDVCAGTNAVGIALLQREPALEVHAIDRSREMQEV
                     GRRRAQARGYVIHGLIGDVHDLPFPDAHF"
     gene            complement(499..840)
                     /gene="frdD"
                     /locus_tag="VFY80_05625"
     CDS             complement(499..840)
                     /gene="frdD"
                     /locus_tag="VFY80_05625"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEX6007420.1"
                     /translation="MARSSKPIVWGPFAAGGTLTAFVTPVLIVITLLAALGHAPELLT
                     YENMRAFVAHGLTKAAIVVVVFLSLWSAAHRLRITAYDFGLRCDALAATVVYATALLG
                     TAAAIYFVWSV"
     gene            complement(843..1235)
                     /locus_tag="VFY80_05630"
     CDS             complement(843..1235)
                     /locus_tag="VFY80_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HEX6007421.1"
                     /translation="MSRRPYVREVPPVRWFFQHPRYVRYLVRELSCLFIGAYTLLLVV
                     ALKRLSEGPAAYALFLESLSSPASIIFHIAALLFAVYHSATWFNLTPKALPVQLGERF
                     VPARVIAGVHYAGWALISLVALALAGAF"
     gene            complement(1232..1975)
                     /locus_tag="VFY80_05635"
     CDS             complement(1232..1975)
                     /locus_tag="VFY80_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX6007422.1"
                     /translation="MSERAVELEVLRYNPETDEAPRFQRYSVNCREEWVVLDALNHVK
                     ETVDPTLNYRWSCHMAVCGSCGMMINGEPKLACKSFLRDYDGVIRVEPLAHFPIERDL
                     VTVIDDFMAKLARVKPYLIPKHDKPVSEGEYLQTPAELKRYKQYTLCINCMLCYAACP
                     EYGLIPKFIGPAAISLAHRYNQDTRDGGRELRQDEIAAAEGVWECSFTGACSEVCPEH
                     VDPASALQQVKIASTIDWYKTHLMPWTKS"
     gene            complement(1972..>2952)
                     /locus_tag="VFY80_05640"
     CDS             complement(1972..>2952)
                     /locus_tag="VFY80_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6007423.1"
                     /translation="RRFLQDYGMGPETPLGKPVLKTMELGPRDRVSQAFWHEQKKGNA
                     VPTQWGDAMLLDMRHLGEKKINERLPLVRDMAVSYMGIDPVKDPVPVRPVVHYMMGGI
                     HTDKRAATPLRGLFAAGECACVSINGANRLGSNSLTELLVFGRRAALSAMEYLSTADR
                     GAHGPALDALADAAQARVRALVQRSGGKESVAGLRREMMQTMEQHAGIYRSREGLTEA
                     CAKLDQLRRRYADIELHDKTNVYNTDLLQALELASMLDCAQAVAVSALARRESRGAHQ
                     RLDFVERDDKHYLKHTLAYHNRAEPPRIDYCDVVITRSQPGVRDYSGGHA"
BASE COUNT          501 a          963 c          986 g          502 t
ORIGIN      
        1 tcgaagtgcg catccgggaa cggcaggtcg tggacatcgc cgatgaggcc gtggatcaca
       61 tagcctcgcg cctgcgcgcg ccgccgcccg acttcctgca tctcgcggct gcggtcgatc
      121 gcatgcactt ccagtgcagg ctcgcgctgc agcagcgcga ttcccaccgc gttggttccg
      181 gcgcacacgt cgagcacccg ggccccgggg cgcaggtcta tcgtatcgag aaagctgcgc
      241 agaaagtgat tccacatgcc caggctggcg acgtggttcg cgctgtcata gtagggcgcg
      301 acgtcggcga atacccggtt caattcctgc gtccagatcc tgctgaagcg ctgttcgcgc
      361 gcgagttcgc gcgccgctac gggtggcgga ggcatggtct cgtctctcca aggcgtatcg
      421 cggcgcgccg tgcggagcac cggcccgtcc gcgacttgtc gaaaccggcg gcctcctcaa
      481 agatgccgca cgcggccatc atacggacca cacgaagtag atcgctgcgg cggtacccag
      541 aagcgctgtc gcgtagacga cggtggccgc tagggcgtcg cagcgcagcc cgaagtcgta
      601 ggccgtgatg cgcaaccgat gggccgcgct ccacagcgag agaaagacca ccacgacgat
      661 ggccgccttg gtgagcccgt gcgcaacgaa ggcccgcatg ttttcgtatg tgaggagctc
      721 cggcgcgtgg ccgagggcgg caagcagcgt aatcacgatc agcaccgggg tcacgaacgc
      781 ggtgagcgtg ccgcccgctg cgaaggggcc ccagacgatg ggcttgctgg accgggccat
      841 cgtcagaacg ctccggccaa ggcgagcgcg acgagcgaga tgagcgccca gcccgcgtag
      901 tgcacgcccg caatgacgcg ggccggaacg aagcgctccc cgagctgcac gggtagcgcc
      961 ttcggcgtca ggttgaacca ggtcgcgctg tgatagaccg cgaatagcag cgctgcgata
     1021 tgaaaaatta tgctcgcggg gctgctgagg ctctcgagaa agagcgcgta ggcagccggg
     1081 ccctcggaga gccgcttcaa tgcgacgacg agcagcagcg tgtaggcgcc gatgaacaga
     1141 cagctcaact cgcgcacgag gtaacgcaca tagcgcgggt gctggaagaa ccagcgcacc
     1201 gggggcactt cgcgaacgta gggtctgcgg ctcacgactt ggtccaaggc atgagatggg
     1261 tcttgtacca gtcgatggta ctcgcgatct tcacctgctg cagcgcgctc gcgggatcga
     1321 cgtgctcggg acagacttcg gagcacgcgc cggtgaagga gcattcccac acgccctcgg
     1381 cggccgcgat ctcgtcctgc cgcagctcgc gcccgccgtc gcgcgtatcc tggttgtagc
     1441 ggtgcgcgag cgaaatcgcg gcggggccga tgaactttgg gatcagcccg tattcgggac
     1501 aggcggcgta gcacagcatg caattgatgc agagcgtgta ctgcttgtac ctcttcaact
     1561 cggcgggcgt ctggaggtat tcgccctcgc tcaccggctt gtcgtgcttc ggaatgaggt
     1621 acggcttcac ccgcgcgagc ttggccatga agtcgtcgat caccgtcacg aggtcgcgct
     1681 cgatgggaaa atgcgcgagc ggctcgacgc ggatcacgcc gtcgtagtcg cgcaggaagg
     1741 acttgcacgc gagcttcggc tcgccgttga tcatcatgcc gcaactgccg cacaccgcca
     1801 tgtggcagga ccagcggtag ttgagcgtgg gatcaaccgt ctccttcacg tggttcagcg
     1861 catcgagtac gacccattcc tcgcggcagt tcaccgaata gcgctggaag cgcggcgcct
     1921 cgtcggtttc cgggttgtat cggaggactt ccagctcgac ggcgcgctcg ctcatgcgtg
     1981 gcctcccgag taatcgcgca cgcccggctg cgaccgggtg attacgacgt cgcagtagtc
     2041 gatgcgcggc ggctcggcgc gattgtggta ggcaagggtg tgcttcaggt agtgcttgtc
     2101 gtcgcgctcg acgaaatcga gcctctggtg cgccccgcgc gactcgcgcc gggcgagcgc
     2161 actcaccgcc accgcctgcg cgcaatcgag catggacgcg agctcgagcg cctgcagcag
     2221 atccgtgttg tagacgttgg tcttgtcgtg cagctcgata tcggcgtaac gccggcgcaa
     2281 ctggtcgagc ttggcgcacg cttccgtgag cccctcgcgg ctgcggtaga tgcccgcgtg
     2341 ctgctccatc gtctgcatca tctcgcgccg cagtcccgcg acgctttcct tgccgccgct
     2401 gcgctggacg agcgcccgga cacgggcttg cgccgcatcc gccaaggcat cgagtgcggg
     2461 gccgtgcgcg ccgcgatccg cggtactgag atattccatg gcgctcaagg cagcgcggcg
     2521 tccgaagacc aagagttcgg tgagggaatt ggagcccagg cggttcgcgc cgttgatgct
     2581 cacgcatgcg cactcgcccg ccgcgaaaag gcccctgagc ggcgtcgccg cacgcttgtc
     2641 ggtgtggatg ccgcccatca tgtaatgcac gaccgggcgc acgggaaccg gatccttcac
     2701 gggatcgatc cccatgtagc tcacggccat gtcacgcacg agcggcaggc gctcgttgat
     2761 cttcttctcg cccaagtgcc gcatgtcgag cagcatggca tcgccccact gcgtgggcac
     2821 ggcgttaccc ttcttctgct cgtgccagaa cgcctggctc acgcggtcac ggggccccag
     2881 ctccatggtc ttgagcactg gcttgccgag cggcgtctcc ggccccatcc cgtaatcctg
     2941 gagaaaacgc cg
//
LOCUS       DASURG010000323         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_57058, whole genome shotgun sequence.
ACCESSION   DASURG010000323 DASURG010000000
VERSION     DASURG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57058"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /gene="glyA"
                     /locus_tag="VFY80_05645"
     CDS             <1..256
                     /gene="glyA"
                     /locus_tag="VFY80_05645"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920551.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6007424.1"
                     /translation="LALGSVGITVNKNAVPHDPQKPTVTSGIRIGTPAMTTRGFREVE
                     AEQLAMLIADVLETPRDEKVIRHVAGEVKRLCEKYPVYRR"
     gene            253..720
                     /gene="nrdR"
                     /locus_tag="VFY80_05650"
     CDS             253..720
                     /gene="nrdR"
                     /locus_tag="VFY80_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX6007425.1"
                     /translation="MKCPFCKSDDTQVIDSRVSDDGDSIRRRRRCTSCNKRFTTYETV
                     ELRMPQVVKQDGTRTEFDLNKLRTGFMRALHKRPVPTQAVDEAIATITQNVLALGERE
                     IDSRRIGEMVMRELHRLDHVAYIRFASVYKSFQGVDDFQHAIKEVQKPVSGKK"
     gene            742..1824
                     /gene="ribD"
                     /locus_tag="VFY80_05655"
     CDS             742..1824
                     /gene="ribD"
                     /locus_tag="VFY80_05655"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX6007426.1"
                     /translation="MSCSDDRRHMARALELAERGLYTTTPNPRVGCVIVREGAVVGVG
                     WHERAGAPHAEANALAMAGAAARGATAYVTLEPCAHYGRTPPCAPALAAAGVARVVAA
                     MRDPNPRVAGQGFAILEKAGVQAQCGVLESEARELNIGFVQRMTRGRPWTRLKIAASL
                     DGKTALENGASQWITGPEARRDGHAWRARACAVLTGIGTVKDDNPELTVREVATPRQP
                     LRIVVDSRLETPPGARVLRDGALIAAAFDEAERSAALRARGAEIVVLPDARGKVELGA
                     LMTELGRRELNEVHVEAGFKLNGSLLREQLVDELVIYLAPHIIGDRARGMFDLPALEA
                     LSGRRPLEIRDTRRVGDDIRVIARPA"
     gene            1824..2429
                     /locus_tag="VFY80_05660"
     CDS             1824..2429
                     /locus_tag="VFY80_05660"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6007427.1"
                     /translation="MFTGIVTAVGEVAASVPSAAGARLRVRASEPFLQDVAVGDSIAV
                     SGVCLTAVTLAGETFEADVSRETLQCTAGFRAGDRVNLEKALRLADRLGGHLVSGHVD
                     GVGEVVLFESAGENRLLTVAVSPDIVRYIARKGSIALNGVSLTVNAVSGVQLSVNLIP
                     HTLQATNLRDLRLGDKVNVEVDLIARYAERLLAVPSDERPD"
     gene            2431..3549
                     /gene="ribBA"
                     /locus_tag="VFY80_05665"
     CDS             2431..3549
                     /gene="ribBA"
                     /locus_tag="VFY80_05665"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX6007428.1"
                     /translation="MPISPITEIIADIRAGRMVVLVDEEDRENEGDLLMAAEFVTPEA
                     INFMARHGRGLICLTLTEERCRQLNLPLMVANNRSPLGTNFTVSIEAAEGVTTGISAA
                     DRARTVQAAIRADASPRDIVQPGHIFPLMAQEGGVLVRAGHTEAGCDLARLAGLTPAA
                     VICEILGESGDMARLPELEGFARHHRLKIGTIANLIEHRSRTESLVKRVTERDIRTAH
                     GQFRLYVYSDRIGEETHLALVKGEPAPEREVLVRVHEPLSVIDLLDTGSTNHSWNFYD
                     ALSAVGRSECGVVLLLHRLETAAELLERARPQRRDTPPPRIALRNYGIGAQILRDLKV
                     RKMRLLALPRPMPSMAGFDLEVVGYVQPPQGGKDKQSA"
     gene            3579..>3830
                     /locus_tag="VFY80_05670"
     CDS             3579..>3830
                     /locus_tag="VFY80_05670"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6007429.1"
                     /translation="MARYDNIEELEPDHSGAGLRIGIVLSRFNQDVGEGLLSSCTAAL
                     TKSGVHESNVQIVTVPGALETPLALQKLAQSGKFDALIAL"
BASE COUNT          689 a         1196 c         1315 g          630 t
ORIGIN      
        1 gctcgcgctg ggcagcgttg gcatcacggt caacaagaac gccgtgcccc acgatccgca
       61 gaaaccaacc gtcacgagcg gcatccgcat tggcaccccg gccatgacga cgcgcgggtt
      121 cagggaggtg gaggcggagc agctggcgat gctgatcgcc gacgttctgg aaacgcctcg
      181 agacgagaag gtgatccggc acgtggcggg cgaagtgaaa cgcctgtgcg agaagtatcc
      241 cgtctaccgt agatgaagtg cccgttttgc aaatcggacg acacccaggt catcgactcg
      301 cgggtaagcg atgacggaga cagcatccga cgacgccggc gctgcacgag ctgcaacaaa
      361 cgcttcacca cctacgagac ggtggagctg cgcatgccgc aggtcgtcaa gcaggacggc
      421 acgcgcacgg aattcgatct caacaagttg cgtaccggct tcatgcgggc actgcataag
      481 cgtcccgtcc cgacgcaggc ggtggacgag gcgatcgcga cgatcacgca gaacgtgctg
      541 gcgctgggag agcgcgagat cgattcgcgg cgcatcggtg agatggtgat gcgagagttg
      601 catcgcctgg atcacgtcgc ctatatccgc ttcgcgtcgg tgtacaagag ctttcagggc
      661 gtggacgact tccagcacgc catcaaggaa gtgcagaaac ctgtgtcagg caagaagtaa
      721 cgctgctgac gagtgcgttg catgtcctgt tccgatgacc gccgccacat ggcacgcgcg
      781 ctcgaacttg ccgagcgcgg cctttatacg acgacgccga atccgcgcgt cggctgcgtg
      841 atcgtgcgcg agggcgcggt cgtcggcgta ggctggcacg agcgcgcggg tgcgccgcat
      901 gcggaagcga acgcgcttgc gatggcgggc gccgcagcac gcggggcgac ggcctacgtg
      961 acgctcgagc cctgcgccca ttacgggcga acgccgcctt gcgcgccggc actcgctgcc
     1021 gccggcgtcg cgcgagtggt cgccgccatg cgcgatccca atccccgcgt ggcggggcag
     1081 gggtttgcga ttctcgagaa ggccggcgta caggcgcaat gcggcgtcct cgaaagcgag
     1141 gcgcgcgagc tcaacatcgg cttcgttcag cgcatgacgc gcggccggcc ctggacacgg
     1201 ctgaaaatcg cggcgagcct agacggcaag actgcgctcg aaaacggcgc gagccagtgg
     1261 attacgggcc ctgaagcgcg gcgcgacggt cacgcatggc gtgcacgcgc ctgtgccgtg
     1321 ctgaccggca tcggcacggt gaaggatgac aacccggagt tgacggtccg cgaagtggcg
     1381 acgccgcgcc agccgctacg catcgtcgtg gacagccggc tggaaacgcc tcccggtgcg
     1441 cgggtgttgc gggacggcgc gctgatcgca gccgccttcg atgaagccga acgcagtgcg
     1501 gccttgcgcg cacggggcgc ggaaatcgtc gtgctgcccg acgcacgcgg caaagtcgag
     1561 ctcggcgcgc tcatgacgga gctcggccgg cgggaattga acgaggtgca cgtcgaggcg
     1621 ggtttcaagc tgaacggatc gctgctgcgg gagcagctcg tggacgagct tgtgatctat
     1681 ctcgctccgc atatcatcgg tgatcgcgct cgcgggatgt tcgatcttcc cgcgctcgag
     1741 gcgttgagcg gccggcgccc gcttgagatt cgagatactc ggcgtgtagg cgacgatatc
     1801 cgcgtcatcg cgaggccggc ctgatgttca cggggatagt gaccgcggtg ggggaagtcg
     1861 ctgcaagcgt gccttcggcg gccggcgcca ggctgcgggt gcgcgcgagc gagccttttc
     1921 tgcaggacgt cgcggtcggc gatagcatcg cggtcagcgg cgtgtgtctc accgccgtga
     1981 cgctcgcggg cgaaacgttc gaagccgatg tgtcgcggga gacgctgcag tgtaccgcgg
     2041 gattccgcgc cggagatcgc gtcaatctcg agaaggcgct gcgtctcgcg gatcgcctgg
     2101 gcgggcacct cgtgagcgga cacgtcgatg gcgtgggaga ggtcgtcctg ttcgagtcgg
     2161 ccggagaaaa ccgcctgctt accgtcgcag tgtcgccgga tatcgtgcgg tatatcgcgc
     2221 gcaagggttc gatcgcgctg aatggcgtga gcctcaccgt caatgccgtg agcggtgtgc
     2281 aactgagcgt gaacctcatt ccgcacacgc tgcaggcgac gaacttgcgc gatctccggc
     2341 ttggcgataa ggtcaacgtc gaagtggatc tcatcgcgcg ttacgcggag cggctgctcg
     2401 ccgtgccgtc ggatgagcgg cccgattgac atgccgatca gccctataac cgaaatcatc
     2461 gccgacatcc gtgccggacg gatggtggtg ctcgtcgatg aggaggaccg cgagaacgaa
     2521 ggcgatctcc tcatggcggc ggagttcgtc acgccggagg cgatcaactt catggcccgc
     2581 cacgggcgcg ggctcatctg cctcacgttg accgaggagc gctgtcgcca gctcaacctg
     2641 ccgctgatgg tggcgaacaa ccgctcgccg ctcgggacca acttcacggt gtcgatcgag
     2701 gcggccgagg gcgtgacgac cggcatttcc gctgcggatc gcgcccgcac cgtgcaggcc
     2761 gcgattcgcg ccgatgcgag cccgcgcgat atcgttcagc cgggacatat cttcccgctg
     2821 atggcgcagg agggcggcgt attggtgcgc gccggccata cggaagcggg ctgcgatctc
     2881 gcgcggctcg cgggcttgac gccggccgcc gtgatctgcg agatcctcgg cgaaagcggc
     2941 gatatggcac ggctgccgga gctcgaggga ttcgcgcgtc accatcgcct caagatcggc
     3001 accatcgcca atctcatcga gcaccgcagc cgcaccgaat cgctcgtgaa aagggtgacg
     3061 gagcgcgaca tccggactgc gcatggccaa tttcgcctct acgtctacag cgaccgcatc
     3121 ggcgaggaaa cgcatctcgc gctcgtcaaa ggggaaccgg ctccggagcg cgaggtgctg
     3181 gtgcgggtac acgagccgct ttcggtcatc gatcttctcg ataccggcag caccaaccac
     3241 tcgtggaatt tttacgacgc gctttcagcc gtaggccgat ccgaatgcgg tgtggtgctg
     3301 ctgctgcacc gcctcgaaac ggcagcggag cttctcgagc gcgcgcgccc gcaacgccgc
     3361 gatacgccgc cgccgcgcat cgcgctacgc aattacggga tcggggcgca aatcctccgc
     3421 gacctgaaag tgaggaaaat gcggcttctc gcgctgcccc ggccgatgcc gagcatggca
     3481 ggcttcgatc tcgaagtggt cggttatgtg cagccaccgc agggtggcaa ggacaagcag
     3541 tcggcttgaa tctccatcgg gtctgaaatc atttcgtcat ggcacgttac gacaatatcg
     3601 aggaactgga accggatcat tccggcgccg ggctccgaat cggcatcgtg ttgagccgct
     3661 tcaaccagga cgttggcgaa gggctgcttt cctcttgcac tgcagcgctt accaaatccg
     3721 gcgtgcatga aagcaacgtc cagatcgtca cggtgccggg cgcgctcgag acgccgcttg
     3781 cgctccagaa actcgcgcag agcggcaagt tcgacgcgct catcgcgctc
//
LOCUS       DASURG010000324         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_670693, whole genome shotgun sequence.
ACCESSION   DASURG010000324 DASURG010000000
VERSION     DASURG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670693"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFY80_05675"
     CDS             <1..202
                     /locus_tag="VFY80_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007430.1"
                     /translation="ALRQAIVSPVAALGQAMEQIVQEWSRRSALDETYIRLASPFYLF
                     EKRSRAHEPQPWHICCTSAWKR"
     gene            417..1562
                     /locus_tag="VFY80_05680"
     CDS             417..1562
                     /locus_tag="VFY80_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6007431.1"
                     /translation="MAQYSRREIACMSRHILITGGAGFIGSHLADALLRRGCDVRVLD
                     SLVSQVHGEHHPDYLDPNVELFHGDLRDSATVHRALRGVDAVYHFAAAVGVGQSMYEI
                     QTYTSINSVGTAVLLEALTAQPVEKLIVASSMSVYGEGLYLGSDGSARIVVERSLEQL
                     KRCAWEFTGSDGAPLEPVATPETKPPSLSSVYALTKYDQECLCLMVGRAYRIPTIALR
                     FFNVYGPRQALSNPYTGVLAIFASRYLNGKAPLIFEDGNQRRDFVSVHDVVQACLLSL
                     DATEMSERIFNVGSGAGYTVRELAERMARALGKEDIAPQISGKYRVGDIRHCFADISL
                     ARAYLGYIPRVGLEDGIVELAAWLDGRTATDRVERASAELAQRGLTL"
     gene            1682..>2891
                     /locus_tag="VFY80_05685"
     CDS             1682..>2891
                     /locus_tag="VFY80_05685"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX6007432.1"
                     /translation="MEVFRAGDRAGVDHVLSGLRALGVTDLRIAVSWADWGTPEGEAW
                     YSWLLPQLASQVNVLPCVLCTPLSQALAPRVSAPPWDPKAYGDFIDVLITRLGHCFEW
                     IELWNEPDRLSEWDRTLDPHWQTFSAMIGGAAYWAQRRGKRTLLAGMNVYDPNWLELM
                     FRRGVMQYIDAVGIHGFPGTCEASWEGWTAMVQSVQQILTLHRSGAEIWISATGYSTW
                     RHDEYEQLRAFAAALEAPVERVYWHSARDLDPEFSARDGFHADDREYHFGLRASDGTP
                     KLLARLWASGGLKAIWRALEPQPGETRRRPKPSQPVLITGGAGFIGTNLANRLAADGE
                     RVLIYDNLSRPGAERNMRWLLDTHPRRVEVEFADVRNAYALRRAVRHAKQVFHFAAQV
                     AVTTSLANPRH"
BASE COUNT          537 a          886 c          898 g          570 t
ORIGIN      
        1 cgcgctgaga caagccatag tttcgcctgt tgccgcgctg ggacaagcga tggagcagat
       61 cgttcaggaa tggtcgcgca ggtccgcatt ggacgaaacg tacattcgtc tggcatcgcc
      121 gttttatttg tttgaaaaaa gatcgcgcgc gcacgagccg caaccgtggc atatatgttg
      181 cacgagcgca tggaagcgat gacacattcg agtacgaccc gcgcgtggaa caatcatgcg
      241 actgttctga ccgagatcca acgaaatttg tcgcctgaag atcgcggcaa cgttgatctg
      301 acctcgcacg cgacgccaac aattgcctgc ggagatatta gtcccagtcc attgggcgac
      361 tgtgcgactc cgcggctgga cttgccgttc gactcgccaa ttcgcacctt tgacgcatgg
      421 cgcagtacag ccgtcgggag attgcctgca tgagtcgcca tatcttgatc accggtggag
      481 ccggcttcat cggctctcac cttgcggatg cgctcttgcg ccgtggttgt gacgtgcgcg
      541 tgcttgactc actggtgagt caagttcacg gagagcacca tccggattac cttgatccca
      601 acgtggaact tttccacggc gatctacgcg acagcgcgac ggtgcaccgc gcgctgcgcg
      661 gtgtggatgc ggtttatcac ttcgcggccg cggtgggcgt cggccagagc atgtatgaaa
      721 tccaaaccta tacgagcatc aattccgtgg gcacggccgt cctgctggaa gccttgaccg
      781 cgcagccggt agaaaaactg attgtggcct ccagtatgag cgtatacggc gagggtcttt
      841 atctcggcag cgacgggagc gcgcgcatcg tggtggaacg ctcgctcgaa cagctcaagc
      901 gttgcgcatg ggaattcacc ggcagtgacg gcgcgccgct cgagccggtc gccactcccg
      961 aaaccaagcc gccgagcctg agctccgtat acgcgcttac caagtacgat caagaatgtt
     1021 tgtgtctgat ggtcgggcgc gcataccgca tcccgaccat agctctgcgc ttcttcaacg
     1081 tttatgggcc gcgccaggcg ttatcgaatc cctatacggg agttctggcg attttcgcgt
     1141 cgcgttattt gaacggtaaa gcgccgttga ttttcgagga cggcaatcag aggcgtgact
     1201 tcgtcagcgt gcatgacgtc gtccaggcat gcttgctgag cctcgacgcc accgaaatga
     1261 gtgagcgcat cttcaacgtc ggcagcggcg caggctacac tgtccgcgag cttgccgaac
     1321 gcatggcacg ggcgctgggt aaggaagaca ttgcgccgca gatcagcggc aaatacaggg
     1381 tgggcgacat tcgtcattgt ttcgccgata tcagtctggc gcgtgcgtat ctgggataca
     1441 ttccacgggt gggtcttgag gatggcattg tggaactggc ggcatggctc gacggacgaa
     1501 cggccacgga ccgtgtcgaa cgtgccagcg ccgagctcgc ccagcgaggt ctcaccctat
     1561 gaaaaaactc gcgcgcgcga cacgtcaaaa tcccgcggtc gcagcgcggc cggtcgcgcg
     1621 tcatccgagc ttacatgttc tgaacggcga gtcggcgaat tcgggcgtac gcgtcggcgt
     1681 cgtggaagtt ttccgagccg gagatcgcgc cggtgtcgac catgttctca gcggcctgcg
     1741 cgccctggga gtgacggacc tgcgcatcgc tgtttcatgg gccgactggg gaactcccga
     1801 aggcgaggcc tggtacagct ggctgctccc ccagctcgcg agccaggtga acgtccttcc
     1861 ttgcgtgctc tgcacaccgc tcagccaagc tctcgcgccg cgtgtttccg ctcctccctg
     1921 ggatccaaaa gcgtacggcg atttcatcga cgtgctgatc acgcggctcg gtcactgctt
     1981 cgaatggatc gagttgtgga acgaaccgga ccgcttgagc gagtgggatc gcaccctcga
     2041 tccgcattgg caaacattct cggccatgat cggcggcgcc gcttactggg cgcagcggcg
     2101 cggcaaacgc acccttcttg ccgggatgaa cgtgtacgac ccgaactggc tcgagctgat
     2161 gtttcggcgc ggcgtcatgc agtacatcga cgccgtcggc atccacggtt ttcccggcac
     2221 ctgtgaagcg agttgggaag gctggacggc aatggtgcaa agtgtgcagc aaattctcac
     2281 gctgcaccgc tcgggagcgg aaatttggat cagcgccacc gggtactcca cctggcggca
     2341 cgacgaatac gagcagttgc gcgcgttcgc cgccgcgttg gaagcgccgg tggaacgcgt
     2401 ctactggcac tcggcgcgcg atttggatcc cgagttttcc gcgcgcgacg gctttcacgc
     2461 cgacgatcgc gaataccatt tcggcttgcg cgccagtgac ggcacgccga agctcctggc
     2521 acggctatgg gccagcggcg gcctcaaagc gatctggcgg gcgttggagc cgcagcccgg
     2581 cgaaacccgc cgccgtccga agccctccca gccggttttg atcacgggcg gcgccggatt
     2641 catcggcacc aacctcgcca atcgccttgc cgccgacggt gagcgcgtgc tcatctacga
     2701 caatttgtca cggccgggcg cggagcgaaa catgcgttgg ctcctggaca cgcatccccg
     2761 tcgcgtggag gtcgaatttg ccgatgtgcg caacgcttat gcgctgcgcc gcgccgtgcg
     2821 ccacgccaaa caggtttttc atttcgctgc ccaagtcgcc gtgaccacga gcctcgccaa
     2881 tccgcgccac g
//
LOCUS       DASURG010000325         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_466027, whole genome shotgun sequence.
ACCESSION   DASURG010000325 DASURG010000000
VERSION     DASURG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466027"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="prpD"
                     /locus_tag="VFY80_05690"
     CDS             complement(<1..990)
                     /gene="prpD"
                     /locus_tag="VFY80_05690"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HEX6007433.1"
                     /translation="MASATNVRPAPDKVLVQIADYVDRYQVRSKLAWETARLTLIDTL
                     GCGFEALSYPACTKLLGPVVPGTVVPNGAHVPGTSYVLDPERAALNIGALFRWLDFND
                     AFYGATVIHPSDTYGGILPVADWLSRVRVAERKQPLCVRDVLEAGVKAYEVMGGLAIE
                     NGYTDVGLDHTILVKIAVTAVATRMLGGTRDEIVNAVSNAWIDGQSLRTYRHIPNTGS
                     RKSWAAGDATSRGVRLALIAQTGEMGYPSVLTAKTWGFYDVLFKGQPFRFTREYGSYV
                     MENVLFKISYPAEFHAQTAVEAAMTLFGQLQAMGKTSEDIASIRIRTHEACIRI"
     gene            complement(1184..2197)
                     /locus_tag="VFY80_05695"
     CDS             complement(1184..2197)
                     /locus_tag="VFY80_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007434.1"
                     /translation="MSRAIFILQEKTAMTFRLSAMLAVLSGALAIGGGGPAMAQAWPS
                     KAVRIIVPFGAGGGTDIQARLLGKFFSESMKQQFLVDNRPGAGGLIGAELAVQSPPDG
                     HTILFTTASLSVNVTLYAKRIKFDPLKDLAPVSWISSTPLVLVVHPSVPAKSVKDLVA
                     LAKKSGKMNAGSNGAGTTSHLSVEMLNQMAGLKVAHIPYKGGGPSMAALVAGETDLLF
                     ATGPVASTQLKSGKVRALAVTTPKKSSAFPDLPPMNTFYKEFESDNWYAMFFPRGTPK
                     PIVDRLNAEIAKALQSKEMKEFMPREALDPVASSPAELEANFKREIARYAKIIKAGNI
                     TVE"
     gene            complement(2228..>2514)
                     /locus_tag="VFY80_05700"
     CDS             complement(2228..>2514)
                     /locus_tag="VFY80_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6007435.1"
                     /translation="DDSVPTGTYLDMCSKLPVVDPAKIDVPTLIMRGQFDGIAGFDDL
                     TEFFKRLPNPDKQFAVMAGISHASFQQINYRLVYHILYSFFSQPEPIYRG"
BASE COUNT          459 a          771 c          806 g          478 t
ORIGIN      
        1 gatgcggatg caggcctcgt gcgtgcggat gcggatgctc gcaatgtctt cgctcgtctt
       61 gcccatcgcc tgcagttgcc cgaagagcgt catcgccgcc tcgaccgcgg tctgggcgtg
      121 gaattccgcc gggtaggaaa tcttgaacag cacgttttcc atcacgtacg agccgtactc
      181 gcgcgtgaac cggaacggct gccccttgaa gagcacgtcg tagaagcccc aggtcttcgc
      241 cgtcagcacc gaggggtagc ccatctcgcc ggtctgggcg atgagggcga ggcgcacgcc
      301 gcggctcgtc gcatcgcccg cagcccagga cttgcgcgag ccggtattgg ggatgtggcg
      361 ataggtgcgc agcgactggc cgtcgatcca ggcattcgag accgcgttca cgatttcgtc
      421 gcgcgtgccg ccgagcatgc gcgttgcgac cgccgtcacg gcgatcttga ccagtatggt
      481 gtgatcgagc ccgacgtcgg tgtagccatt ttcgatcgca agcccgccca tcacctcgta
      541 ggccttcacg cccgcttcga gaacgtcgcg cacgcaaagc ggctgcttcc gctccgccac
      601 ccggacgcgc gaaagccaat cagccacagg caggatgccg ccataggtgt cggacggatg
      661 gatgacggtt gcgccgtaga acgcatcgtt gaagtcgagc cagcgaaaga gcgcgccgat
      721 gttgagcgcg gcgcgttcgg gatcgagcac gtacgacgtt ccgggcacat gcgcgccatt
      781 cggcacgacg gtccccggga cgaccgggcc cagcagcttg gtgcacgccg gatacgagag
      841 cgcctcgaat ccgcatccga gggtatcgat gagcgtgagg cgtgccgtct cccaggcgag
      901 cttgcttctg acctggtagc ggtcaacgta gtcggcgatt tgaacgagga ctttgtcggg
      961 tgcggggcgg acgttggtcg cggatgccat ttctcttgtt gaccttcgtt tgtattcgga
     1021 atagctcggg ccgcattata gacgtacata aaaagcgctc ccgacgcaga ggagcgctgg
     1081 acttcacccc ggactaaact tttccggggc atgctgtatg tcgccggact gacgactcgc
     1141 caatcggctt cagccgcaac gaagacttgc cagattggct ggcttactcc acggtgatgt
     1201 tcccggcctt gatgatcttc gcgtaacgcg caatctcgcg cttgaagttg gcttccagct
     1261 cggccggaga actcgccacc ggatccagcg cctcgcgcgg catgaactcc ttcatctcct
     1321 tgctctgcag cgccttggcg atctccgcgt tcagccggtc gacgatcggc ttgggcgttc
     1381 cgcgggggaa gaacatcgcg taccagttgt ccgactcgaa ttccttgtag aaagtattca
     1441 tcggcgggag atccgggaac gcggaggact tcttcggggt agtcacggcg agtgctcgca
     1501 ccttgccgga cttcaactgg gtcgatgcga cggggccggt agcaaacagc aggtcggtct
     1561 cgcctgcgac gagcgccgcc atcgacggtc cgccgccctt gtagggtata tgtgccactt
     1621 ttaacccggc catctggttc agcatctcca ccgaaagatg acttgtggta cccgcgccgt
     1681 tcgagcccgc gttcatcttg ccggatttct tggcgagcgc aaccaagtcc ttcaccgatt
     1741 tggccggcac gctcggatgc acgacgagga cgagcggcgt ggacgagatc cagctcaccg
     1801 gagcgagatc cttgagcgga tcgaacttga tgcgcttcgc atagagcgtg acgttcaccg
     1861 aaagcgacgc cgtggtgaag agtatggtgt ggccgtcagg cggcgactgg accgcaagct
     1921 cggcgccaat caggccaccc gcgcccggcc gattgtcgac caggaactgc tgcttcatgc
     1981 tttcgctgaa gaacttcccg agcagccgcg cctggatatc ggtgccaccg ccggcgccga
     2041 agggcacgat gatgcgaacc gccttgctgg gccaggcctg cgccatggcc gggccgccgc
     2101 cgccgatcgc gagcgcaccg gacaatacag cgagcatcgc gctcaatcta aaggtcatgg
     2161 cagttttctc ctggaggatg aaaatcgcgc gggacaccgg cgcgccggac gcagcagccg
     2221 ttggacacta tccccggtaa atcggttcgg gctggctgaa gaacgaatac aggatgtggt
     2281 agaccagccg gtaattgatc tgctggaagc tcgcgtgcga gatgccggcc atcaccgcga
     2341 attgtttgtc cggattgggc aggcgcttga agaattcggt gaggtcgtcg aagcccgcga
     2401 tgccgtcgaa ctgcccgcgc atgatgaggg tgggcacatc gatcttcgcc ggatcgacca
     2461 ccggaagctt cgagcacatg tcgaggtatg ttccggtcgg gaccgaatcg tcga
//
LOCUS       DASURG010000326         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_976817, whole genome shotgun sequence.
ACCESSION   DASURG010000326 DASURG010000000
VERSION     DASURG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976817"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1322)
                     /locus_tag="VFY80_05705"
     CDS             complement(66..1322)
                     /locus_tag="VFY80_05705"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HEX6007436.1"
                     /translation="MTGMTIAEKILAKKSGQATVRSGDLVTVEVDTVILFDNNFMPSQ
                     WRDILKLKDPKRVIVVLDHRVPAATQASAAAHRTAREFVARFGIPRFHDLGYDQGISH
                     QLVADHGYALPGTVLLCSDSHTCSAGVFNCIARGVGAPDVMLAATKGETWFRVGETVR
                     YELEGRLPRAVTMKDAFLQIAGKHGDHATMNVEYGGPGVAALSINARKTLTTMSAELS
                     AEFATFEPDEVMLEWVRARNPMPFEAFHPDADARYADVRHVDLSSLEPLVALPDSVIE
                     NSRPVAAAAGTPIDQAFVGSCANGTLDDLELTASVLGGRRVSPKVRFIVTPASQNVYR
                     EAARRGIVGTLVDAGAVVTPATCGACGGGHMGVLGPNETCITATTRNFKGRMGDASAR
                     IFMGSPATVAASAIRGVITDPREFLN"
     gene            complement(1476..2366)
                     /locus_tag="VFY80_05710"
     CDS             complement(1476..2366)
                     /locus_tag="VFY80_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6007437.1"
                     /translation="MISKGKQLKELIHSPKILVTPGVYDGYSARLVQKFGFRTGSISG
                     AGVSESKIGWADRGVMGYGENVEACRRIAAFSDLLLQGDADTGYGNALNVHFTVRGFE
                     DAGLAAIMIEDQVWPKRCGHMAGKAVIPAEEMVQKIKAAVSARRDPDFVIKARTDAAG
                     PHGIEEAIRRLNMYAEAGADCLFADALLSGKDIERVARSVPKPLSVNMGLGIRSRPTT
                     PLLSPRQLEEMGVAQVSYPRLLSTAALKGMMNAMQVFRDEVVEKGNVVDRPDLLVSFG
                     EINELMGLNELEVLEQQFAS"
BASE COUNT          415 a          825 c          749 g          462 t
ORIGIN      
        1 atgttgtccc cgaccttcca cacccgggcg gtaaatttca tctgcgctcg cttcctcgca
       61 tcgccctaat tcaggaattc gcgcgggtcg gtgatgacgc ctcggatggc ggacgccgca
      121 acggtcgcgg gcgagcccat gaaaatgcgg gcgctcgcgt cgcccatgcg ccccttgaag
      181 ttgcgcgtgg tcgcggtgat gcacgtctcg ttcggaccca gcacgcccat gtgcccgccg
      241 ccgcacgccc cgcacgtcgc aggcgtcacg accgcgcccg catcgacgag cgtgccgacg
      301 atgccgcggc gcgccgcttc gcgatatacg ttctgcgagg cgggtgtcac aatgaagcgc
      361 accttcggtg aaacgcgccg ccctcccagc acgctcgcgg tcagctcgag atcatcgagc
      421 gttccgttgg cacacgagcc gacgaatgcc tgatcgatcg gcgtgcctgc tgcggcagcg
      481 acgggccggg aattctcaat cacgctgtcc ggtaatgcga cgagcggctc gagactggaa
      541 agatccacat gacgcacgtc ggcatagcgc gcatccgcat ccggatggaa ggcttcgaat
      601 ggcatcgggt tgcgcgcccg cacccattcg agcatgactt cgtcgggttc gaaggtggcg
      661 aattccgccg acagctcggc cgacatcgtg gtcagcgttt tgcgcgcatt gatgctcaat
      721 gccgcgacgc ccgggccgcc gtactcgacg ttcatggtcg catgatcacc gtgcttgccc
      781 gcgatctgga gaaaggcgtc cttcatcgtc accgcgcgcg gcaaacggcc ttccagctcg
      841 tagcgcaccg tttcaccgac ccggaaccac gtttcgccct tcgtcgcggc aagcatcacg
      901 tcgggcgccc ccacgccgcg tgcgatgcag ttgaagacgc cggcactgca ggtatgagaa
      961 tcgctgcaga ggagcaccgt ccccggcaag gcgtagccgt ggtccgccac gagctgatgg
     1021 ctgatgccct ggtcgtagcc gagatcgtga aagcgcggga tgccgaatcg tgcgacgaat
     1081 tcgcgcgccg tgcgatgcgc cgccgcgctc gcctgcgtcg cggccggcac acggtggtcg
     1141 agcacgacga tgacgcgctt cgggtccttc agtttgagga tgtcgcgcca ctggctcggc
     1201 atgaaattgt tgtcgaacag gatcacggtg tccacttcga ccgtgacgag atcgccggag
     1261 cgaacggtcg cctggccgct tttcttcgcc aggatctttt ccgcgatcgt catccccgtc
     1321 atgcccgctc ccaaacaaat tcgtcactcc ggcgaaagcc ggagcccagg aaagagacaa
     1381 acgaatcgaa cttcatctgg ataccggccc cggcttgaac catgccgggg catgcttcgc
     1441 cggtatgacg agccgtgtct gatacgccat ggcagtcatg aggcgaactg ctgttcgagc
     1501 acttcgagtt cgttaagccc catcagctcg ttgatctcgc cgaacgagac gaggagatcg
     1561 gggcgatcga cgacgttgcc cttctccact acttcgtccc tgaagacctg catcgcgttc
     1621 atcatgccct tcagcgcggc ggtggaaaga agccggggat agctcacctg cgcgacgccc
     1681 atctcttcga gctgccgcgg cgaaaggagc ggcgtcgtgg ggcgcgatcg gatgccgagc
     1741 cccatgttga cggagagcgg cttaggcacg ctgcgcgcga cgcgttcgat atccttcccg
     1801 gagagcagcg catcggcgaa aaggcagtcg gcgcccgcct cggcatacat gttcagccgt
     1861 cggatcgctt cttcgattcc gtgcggcccc gccgcgtcgg tgcgcgcctt gatcacgaaa
     1921 tcgggatcgc gccgcgcgga caccgcggcc ttgatcttct gcaccatttc ctccgcggga
     1981 atcacggcct ttccggccat gtgaccgcag cgcttcggcc aaacctgatc ctcgatcatg
     2041 atggccgcga gccccgcgtc ctcgaagccg cgcacggtga aatggacgtt gagcgcgttg
     2101 ccgtatcccg tgtccgcgtc gccctgcagc agcaggtcgc tgaaggccgc gatcctgcgg
     2161 cacgcttcca cgttttctcc gtagcccatc acgccgcgat cggcccaccc gatcttgctt
     2221 tcggatacgc ccgcgcccga gatgctgccg gttcggaagc cgaacttctg cacgagccgc
     2281 gcgctgtagc cgtcgtagac gccgggtgtc acgagaatct tcggcgagtg gatgagctct
     2341 ttgagctgtt tccctttgct gatcatcgaa acggtcctct cttctgaggc gatctcacgc
     2401 cgcgccgtcg ggcttcaagc ccagcgtgcg aatcaccttc gtccattttt c
//
LOCUS       DASURG010000327         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1181246, whole genome shotgun sequence.
ACCESSION   DASURG010000327 DASURG010000000
VERSION     DASURG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181246"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFY80_05715"
     CDS             complement(<1..228)
                     /locus_tag="VFY80_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX6007438.1"
                     /translation="MTDELPFIDPTAGRTRPTIPLAVRPGDLAGKVVGLLDNSKEQAD
                     VILETVGAALRERYGVARIVMRRKPFFSKPAT"
     gene            complement(289..1308)
                     /locus_tag="VFY80_05720"
     CDS             complement(289..1308)
                     /locus_tag="VFY80_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007439.1"
                     /translation="MTAVPDEFEFFLEKEWSDGFPVVTPTQARVRRMLEGTHRDQEEL
                     VAHVPPAGEPATVRTVAIHALMAGCKPQYLPVVLGALEIIAREEFNMGGVQCTMHGVA
                     PLMIVNGPYAQKIGLHGGAGCFGPGFRANATIGRAIRLMLLNLGGGIAGSASATVFSS
                     PIRYTACLTEHMAESPWESLAESRGYAPNDNVITCAMVESPRLHFDDVSTEPNRLLAG
                     IADAMTAPGSWNMWFTSDMVVAMSPQHARLCAEAGLSRADVVKHLAESAVRSQRNLKR
                     GGNWRPERARAMGLDPDDDDALVKAVKDPARLHLIVAGGLGPITAVCHGWNESSRFVH
                     GKYEA"
     gene            1552..2820
                     /locus_tag="VFY80_05725"
     CDS             1552..2820
                     /locus_tag="VFY80_05725"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6007440.1"
                     /translation="MKRGHSIERTTSHGAQPQRDSGHWSVLGGLGIGAAIMYFLDPER
                     GRRRRALLRDQFVHAGRRLREARRITAVDIANRSSGLWAETSRWFREHSAASDRELVE
                     RVRSKLGRVVSHPHAIDVSVRDGHVTLSGPILMEEVHPLISCVANAEGVRSVEDRLSA
                     YAEAGRVSALQGGRLRANRFELFQRNWSPAARVLVGTVGAGLMLGGMRARGAQCVALE
                     ALGSALLLRAATNRELTALIGVGDVNGGVTVQKTINVNAPVDRVFAFWRDYQNFPRFM
                     TNVRDVQPIDEACSRWIVAGPAGVPIEWVSRLTQVVPNERIEWRSEPGSPVRHGGVVR
                     FYPNGNGGTRVDIQLAYVPPAGAIGHAVAKVFGADPKREMDADLMRMKTMIETGHRPH
                     DAAWPATSVTTAASASEAERAFAAAHPGSK"
     gene            2851..3192
                     /locus_tag="VFY80_05730"
     CDS             2851..3192
                     /locus_tag="VFY80_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007441.1"
                     /translation="MAIGWLTVLKALPWGTILSQAPTVVDAANRLLSQTRRRPSDEAP
                     SGLDALHERIEALEEHDKADAAVMKQLAEEVAVLAQASQVMAIRMRLILALSVAGIAI
                     GCVALAIVILR"
     gene            3241..3693
                     /locus_tag="VFY80_05735"
     CDS             3241..3693
                     /locus_tag="VFY80_05735"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX6007442.1"
                     /translation="METTWIVVADRSRARIFEEVRSPRSLQEIADFVNPLGRASHREL
                     TSDAAGRSRSTGLGTRAHSESPGTTQDTHEAERFARELADHLEKARTAHRYEKLWLVA
                     APRFLAMLRKSVSREVMKLIEQEIDKDLTRSTLLEIDSLIAGRRKPAS"
     gene            3790..4425
                     /locus_tag="VFY80_05740"
     CDS             3790..4425
                     /locus_tag="VFY80_05740"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX6007443.1"
                     /translation="MHRGTVSEHAKARTEWPWWLGVILAGAAYVILKWIIPSLAGSSG
                     PLKPLVATAQSSAALVAIMFLAYAALSLGLAFRRRRALDLETSLATLRALPRERFAHF
                     LEAAFRREGYEMSSPASGDSAIDAVLTRGNERLLVQYRHWRNATLGAAMLQALCDCAG
                     EESAAGCVFVTAGNYTPEAVRFAAGKPVRLIGGRQLERMLRDLERSSGPLR"
     gene            4537..>4796
                     /locus_tag="VFY80_05745"
     CDS             4537..>4796
                     /locus_tag="VFY80_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007444.1"
                     /translation="MRNVLIALALAGAALPAPAEQQKSYAVYMAIPCEAYLQERWDPE
                     GVRYKQIQAWISGYVTSYNGWQTDTHDILGNVSVGEVEAWVEG"
BASE COUNT          831 a         1527 c         1585 g          853 t
ORIGIN      
        1 ggtggcgggc ttcgagaaaa agggtttgcg gcgcatgacg atgcgggcga cgccgtagcg
       61 ctcgcgtagc gccgcgccca ccgtctcgag aatgacgtca gcctgctcct tgctgttatc
      121 gagcagtccc accaccttgc ctgcgagatc gccgggccgc accgcgagcg gtatcgtcgg
      181 ccgcgttctg ccggcggttg gatcgatgaa cggcaattcg tcagtcatac gcgcttcctt
      241 tgagcagttc cagcgtcgag agcgtcgtca tgcgccagcg gcaggggctc atgcctcgta
      301 cttgccgtgc acgaaccgac ttgattcgtt ccagccatgg cacactgccg tgatgggtcc
      361 cagtccgccg gccacgatga ggtgcagccg cgccgggtcc tttaccgctt tcaccagggc
      421 atcgtcatca tcggggtcca gccccatggc gcgcgcgcgc tcggggcgcc agttaccgcc
      481 gcgcttcagg ttgcgctggg agcgcacggc gctttccgcg agatgcttta cgacgtcagc
      541 acggctcaag cccgcttcgg cgcaaaggcg cgcatgctgc gggctcatcg ccaccaccat
      601 atccgaggtg aaccacatgt tccatgaccc cggtgcggtc atggcgtcgg cgatgcccgc
      661 gagcaagcga ttcggctcgg tactcacatc gtcgaagtgc aggcgcggac tttccaccat
      721 cgcgcacgtg atcacgttgt cgtttggcgc gtacccgcgc gactccgcca ggctttccca
      781 aggactctcc gccatgtgct cggtgagaca ggcggtatag cggatcggcg aagaaaatac
      841 ggtcgcagaa gcgcttccgg cgatcccgcc gcccagattg agcagcatga ggcggatggc
      901 gcggccgatg gtggcgttcg cccggaagcc gggaccgaag cagccggcgc cgccatgaag
      961 gccgattttc tgcgcgtacg gtccgttcac gatcatgagc ggtgccacgc cgtgcatcgt
     1021 gcattgcacg ccacccatgt tgaactcctc gcgcgcgatg atttcgagcg ctccgagcac
     1081 gacgggcagg tattgcggct tacaccccgc catcagcgcg tggatcgcta cggtgcgtac
     1141 cgtcgcgggc tctccggcgg gcggcacatg ggcgacgagt tcttcctgat cgcggtgcgt
     1201 cccctccagc atccggcgca cgcgcgcttg cgtgggcgtg acgaccggaa agccgtccga
     1261 ccactctttc tcgagaaaaa attcgaattc atccggcact gcagtcacga caacctcttt
     1321 cagttgagcg gcaatgatag ccgggttacg gccgccgcgt cggctcgctt ttgcgattga
     1381 aaccggctca ttcgatccag caggccacgc ccctgcctcg caaaccgctc cttaagccgg
     1441 attttatccg ggtgcgagta gcaagcgtca gaactgccgc cgtggcacgc ggtcagaacg
     1501 attacggcga aagcattccc atccctgcaa tattcaccca aggagcgggc gatgaaacgc
     1561 ggacattcaa tcgaacgcac cacctcccac ggtgctcaac cccagcggga ttccggccac
     1621 tggtccgtgc ttggcggact cgggattggc gctgcgatca tgtacttcct ggaccccgag
     1681 cgcggtcggc gacggcgtgc gctgctacga gatcagttcg tgcatgcagg gcggcgactg
     1741 cgcgaggcac ggcgcataac cgccgtggat attgccaacc gcagcagcgg tttgtgggcg
     1801 gaaaccagcc gctggtttcg cgagcacagt gctgcctcgg accgcgagct cgtcgagcgc
     1861 gtgcgctcga agctggggcg cgtcgtgtcc cacccgcacg cgatcgacgt gtcggtgcgc
     1921 gacggacacg tcacgctgag cggacccatt ctgatggaag aagtgcatcc gctcatctcc
     1981 tgcgtcgcga acgcagaggg tgtgaggagc gtcgaagatc ggctctcggc gtacgccgag
     2041 gcagggcgcg tgtcagcctt gcagggcggc cggctgcgcg ccaatcgctt cgaacttttt
     2101 cagcgcaact ggtcgcccgc ggcgcgggtg ctggtcggca cggtcggtgc gggactcatg
     2161 ctgggcggca tgcgggcacg cggcgcccag tgtgtcgccc tcgaggcgct tggcagtgcg
     2221 ttgctgctgc gcgcggcgac gaaccgggag ctcactgcgc tcatcggtgt gggcgatgtg
     2281 aacggcggtg tgaccgtgca gaagacgatc aacgtgaacg caccggtcga tcgcgttttt
     2341 gcgttctggc gcgattatca gaacttccca cggttcatga cgaatgtgcg cgatgtgcag
     2401 cccatcgacg aggcgtgttc gcgctggatt gtagcgggcc ccgccggcgt gcccatcgaa
     2461 tgggtgtcgc gtctgacgca ggtcgttccc aacgagcgca tcgaatggcg ctccgagccc
     2521 ggctctcccg tgcgtcacgg aggggttgtg cgcttctacc cgaacggcaa cggcggcacg
     2581 cgagtcgaca tccagctcgc gtacgtgccg ccggccggtg ccatcggtca tgcggttgcc
     2641 aaagtgttcg gcgcggatcc gaagcgcgaa atggacgccg acctgatgcg catgaagacc
     2701 atgatcgaaa ccggacaccg gccgcacgat gccgcatggc cggcgacatc tgtgacgacc
     2761 gccgcatcgg cgagcgaagc tgagcgtgct ttcgcagcgg cccatcctgg ttcaaaataa
     2821 tgcaatgagc atcgtgatgc gcatgggccc atggcgatcg gctggttgac ggtgctgaag
     2881 gcgctgccgt ggggcaccat tttgagccag gcccccaccg tcgtcgatgc ggccaaccgc
     2941 ctgctctcgc agacgcggcg ccgtccgagc gatgaggcgc cttccgggct cgacgcgctg
     3001 cacgagcgga tcgaagcgct cgaagagcac gacaaggccg atgcggccgt catgaagcag
     3061 ctcgccgaag aagtcgccgt gctggcgcag gcctcgcagg tcatggcgat ccgcatgcgg
     3121 ctcatcctgg cgctcagcgt cgcaggcatc gcgatcggat gcgttgccct tgcgatcgtc
     3181 attttgcgct gatttcggcg cacgcgagaa caaaatgccg catgccgtgt ccatgtcgtc
     3241 atggaaacca cctggatcgt cgtcgccgac cgcagcaggg cgcgcatctt cgaagaagtg
     3301 cgttcgcccc ggtcgctgca ggaaatagca gatttcgtaa atcctctcgg gcgcgcgagc
     3361 catcgcgagc tgacgagcga tgctgcgggg cgctcgcgca gtaccggcct cggcacgcgt
     3421 gcgcactccg agtctccggg gaccacgcaa gacactcacg aggcggaacg ctttgcccgc
     3481 gaacttgccg atcatctcga gaaggcgcgc accgcccatc gctacgagaa gctgtggctc
     3541 gtcgctgcac cgcggttcct tgcgatgctg cgaaagagcg tgagccggga agtgatgaag
     3601 ttgatcgagc aggagatcga caaggacctc acgcgttcga cgttgctcga gatcgattcg
     3661 ctcatcgcgg gccgaagaaa gccggcgtca taaggcacgc cgtgaggcac ctccagccgc
     3721 gctggattac cgattacaat atgcccctgt gctaagtgac ggcggcatgt tgtgccgcgg
     3781 ggagcgaaga tgcacagagg cacagtgtcc gagcatgcga aggcgcggac ggaatggccg
     3841 tggtggctgg gcgtcattct ggccggcgcc gcgtacgtca tactcaaatg gatcattccg
     3901 tcgcttgcgg gatcgagcgg accgctgaag cccttggtcg ccaccgcgca gtcttccgcc
     3961 gcactcgtcg cgatcatgtt cctcgcctac gccgcgctct ctctcggcct tgcgttccgc
     4021 cggcggcgcg cgctcgatct ggagacgagc ctcgcgacgc tgcgcgcgct gccgcgcgag
     4081 cgattcgcgc atttcctgga ggcggcgttt cgaagggaag gctacgagat gtcctcgccg
     4141 gcgagcggcg attccgccat cgacgccgtg ctcacgcggg gaaacgaaag actgctcgtt
     4201 caatatcgac actggcgcaa tgcgacgctg ggcgccgcga tgcttcaggc gctgtgcgac
     4261 tgcgcggggg aagagtccgc agccggatgc gtcttcgtca ccgccggtaa ttacacaccc
     4321 gaagcggtgc ggttcgcagc cggcaaaccc gttcgactga tcggcggacg gcaactcgag
     4381 cgcatgttgc gcgacctgga gcgttcctcc ggcccgcttc ggtagggcgg ctgctcccaa
     4441 gctcgctacc tccaaaaaaa tgttgcgcga ggctgtcgcg ccgcgcggcc gcggatcggc
     4501 ggaagctgct attatcgcgt ggcaagggag ggtactatgc gcaacgttct gatcgcgctg
     4561 gcgcttgccg gtgccgcgtt gcccgcgcct gcggagcagc agaaaagcta tgcggtctac
     4621 atggcgattc catgcgaggc ctaccttcag gagagatggg atcccgaagg ggtgcgatac
     4681 aagcaaatcc aggcatggat ctccggctat gtgacttcct ataacggctg gcagaccgat
     4741 acgcacgaca tactgggcaa tgtctcggtc ggggaagtcg aggcctgggt ggaggg
//
LOCUS       DASURG010000328         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1283500, whole genome shotgun sequence.
ACCESSION   DASURG010000328 DASURG010000000
VERSION     DASURG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283500"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFY80_05750"
     CDS             complement(<1..740)
                     /locus_tag="VFY80_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6007445.1"
                     /translation="MSSYTAKRLRSETTCVMDTSAVSAALDVSPERGLAEDEAARRLA
                     QHGPNELRAAASVPRWRRLVAQFEDPLIYLLIGAIVISLAAWLAEGRHGLPVDAFVIA
                     LIVIVNAILGYVQETKAESAVAALARMTAVTSSVLRGGVVKRLESAALVPGDILVLGE
                     GDSVGADARLVQCASLRVQEASLTGESEPVLKDAATLPAPAPLGDRSNMVFKGTAVVQ
                     GSGRALVTATGMDTEVGSIAELLETTTE"
     gene            815..1678
                     /locus_tag="VFY80_05755"
     CDS             815..1678
                     /locus_tag="VFY80_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6007446.1"
                     /translation="MTPYQALWLSIGAALTTIALKWSAAWVTGSVGFLSDALESAVNL
                     LGASFALAMVIYARKPADPGHPFGHGKAEYFASGFEGALIALAAAAILYAAGQRIAYP
                     EPLHTLGVGTVLSIAAGLVNLVVARVLLRVGRAHRSLATEADGRHLMTDVWTTAGVVV
                     GVGLAGWTGYHWLDPVVAILVAFNILLEGWRLVWRSVHGLMDAALGDEDIRGLEAALA
                     RLESGETAFANLRTRRAGTDRFAFVELHVPAGWSVERADELARGAERAASEQGIALAV
                     RLVPADKRASP"
     gene            complement(1713..>2312)
                     /locus_tag="VFY80_05760"
     CDS             complement(1713..>2312)
                     /locus_tag="VFY80_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6007447.1"
                     /translation="SGRVSVPILWDKRTAAIVNNESADIIRMLNSAFGTPTIDYYPAA
                     LRSEIDEVNALVYENVNNGVYRCGFAGKQAAYEAAARRLFDAVDVLEQRLSRSRYLVG
                     NTLTEADWRLFTTLVRFDAVYYGHFKCNVRRIEDYPSLSGYLRDLYQIHGIAETVNFD
                     HIKRHYYMSHTHLNPGRIVPVGPALDFTRPHDRMRLASA"
BASE COUNT          381 a          750 c          747 g          434 t
ORIGIN      
        1 tcttccgtgg tggtttcaag gagttcggca atggaaccga cttccgtatc catgccggtt
       61 gccgtgacga gcgcgcgtcc gcttccctgc acgaccgcgg tgcccttgaa aaccatgttg
      121 ctgcggtcgc cgagcggcgc cggcgctggg agcgtcgcag cgtccttcag cacaggctcg
      181 ctttctcccg tgagggaggc ttcctgaacg cgcagtgacg cgcactgcac cagccgtgca
      241 tcagcgccga ccgaatcgcc ctcgcccagc acgagaatgt cgcccgggac cagcgctgcg
      301 ctttcgaggc gtttcacgac gccgccgcgc agcactgaag aggtgacggc cgtcatgcgt
      361 gcgagcgcag ccaccgcgct ctccgctttg gtctcctgca cgtaacccag gatcgcattc
      421 acgatgacga tcagcgcaat gacgaacgca tcgaccggca ggccgtgtcg gccctcggcg
      481 agccaggcgg cgagcgaaat cacgattgcg ccgatcagca ggtagataag cggatcttca
      541 aactgcgcca caaggcgccg ccaccgcggt accgaggcgg ccgcacgcag ctcgttgggt
      601 ccatgctgcg cgaggcggcg cgccgcttca tcttcggcaa gtccccgctc gggactgaca
      661 tcgagcgccg cgctcactgc cgacgtatcc atgacgcacg tcgtctcgct gcgcaatctc
      721 ttggcggtgt acgaactcat caaagcatcg ctgtctgcgc tacgggcgat atcgcgacat
      781 aatggagtct gcccgcagaa cactctactg tcgaatgact ccctaccagg cgctgtggct
      841 ttcgatcggc gcggcgctca ccaccatcgc gttgaagtgg agcgctgcct gggtcaccgg
      901 ctccgtcggc tttctgtccg atgcgctgga atcggcagtc aacctgctgg gcgcctcctt
      961 cgccctggcc atggtgatat atgcacgtaa gcccgccgac ccggggcatc cgttcggaca
     1021 cggaaaggcc gagtatttcg cttcgggatt cgaaggtgcg ctgatcgcgc tcgccgcggc
     1081 cgccatactt tatgccgccg gacaacgcat cgcgtatccc gaaccgctgc acacactcgg
     1141 cgtaggcacg gtgctttcca ttgcggcggg actggtgaac cttgtcgtcg cccgcgtgct
     1201 gctgcgcgtg ggccgtgcgc accgctccct tgccaccgaa gcggatggcc ggcatctcat
     1261 gacggacgtg tggacgacgg ccggcgtggt cgtcggtgtc ggcctcgccg gttggaccgg
     1321 ctaccactgg ctcgatccgg tggtcgcgat actggtcgct ttcaacatcc tgctcgaagg
     1381 ctggcggctg gtctggcgtt cggttcacgg tctcatggac gccgcgctcg gcgacgagga
     1441 cattcgcgga ttggaagcgg ctcttgcacg gctcgaatcg ggcgagacgg cttttgcgaa
     1501 cctgcgcacg cggcgcgcgg ggaccgaccg cttcgctttc gtggagctcc acgtgccggc
     1561 tggctggagc gtcgagcgcg cggatgagct tgcgcgcggc gccgagcgcg ctgcatccga
     1621 gcagggtatc gcgctcgcgg tgcggctagt gcccgccgac aagcgtgcgt cgccgtgagc
     1681 cagcacgcgc tctgcagccg catccgcgtc cctcaagcgg acgcgagtcg cattcgatcg
     1741 tgcggcctgg tgaaatcgag cgccggaccg acgggcacga tgcggcccgg gttgaggtgc
     1801 gtgtggctca tgtagtaatg gcgcttgatg tgatcgaagt tcaccgtctc cgcgatgcca
     1861 tggatctgat acagatcacg gaggtatccg gaaaggctcg gatagtcttc gatccggcgc
     1921 acgttgcact tgaagtgccc gtaatagacc gcatcgaaac gcacgagcgt ggtgaaaaga
     1981 cgccagtcgg cttcggtcag cgtattgccg acgagatagc gcgatcgcga aagccgctgc
     2041 tcgagcacat cgaccgcatc gaagagacgc cgtgccgccg cctcgtaggc cgcttgtttg
     2101 ccggcgaacc cgcagcgata aacgccgttg ttgacgtttt cgtacaccaa cgcgttcact
     2161 tcgtcgattt cgctgcgtaa tgcggcggga tagtaatcga tggtcggcgt gccgaacgcg
     2221 ctgttcagca tgcggatgat gtccgccgat tcattgttca cgatcgcggc ggtgcgcttg
     2281 tcccacagta ttggcaccga cacgcgcccg ga
//
LOCUS       DASURG010000329         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_874472, whole genome shotgun sequence.
ACCESSION   DASURG010000329 DASURG010000000
VERSION     DASURG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874472"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFY80_05765"
     CDS             complement(<1..213)
                     /locus_tag="VFY80_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007448.1"
                     /translation="MKKRLGAQSRCAVASAAVWALALGLSGCFVAQIVPTAQEHGISL
                     RAGDLKNSGIAFLTPSSSTGQEEEKQA"
     gene            513..827
                     /locus_tag="VFY80_05770"
     CDS             513..827
                     /locus_tag="VFY80_05770"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX6007449.1"
                     /translation="MSKTKRSGNKAPDTPKSEPQAMAGDGATRLTEDERRRMVAEAAY
                     YRALQRGFAAGGEVDDWLAAEREINDQFAAAGNHIVARNAAPARAKGKAASARSAARS
                     VQ"
     gene            complement(816..1859)
                     /locus_tag="VFY80_05775"
     CDS             complement(816..1859)
                     /locus_tag="VFY80_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6007450.1"
                     /translation="MQTVPYNAPLDAWKIPEEDFPSGEAPERQLEFLLRYAILAPSTH
                     NTQPWRFRIRGATLEVLADYDRSLPIADPQDRELIMSCGAALFNLRTAMHYFGCASEA
                     RPFPDAHRPGVVARVALQAGSGQKSEWSELFHAIPRRMTDRGRYSVEAVSGELQAQMC
                     EVARIQGAWLAPFASTRSKEMVADLIATGDRAQFENRAFRAELASWLHSPRDNDGLPA
                     HAKGARQLLGVAIPAVAFLVRTFDLGNGVAARDRALATGSPLLACLGTARDTALDWLN
                     AGQALQRVLLTVTANGLHASFLNQPIEVKELRSALGSLCGQQGYPQVLLRIGRGSGTR
                     HTPRRPVSEVLLH"
     gene            2048..>2344
                     /locus_tag="VFY80_05780"
     CDS             2048..>2344
                     /locus_tag="VFY80_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007451.1"
                     /translation="MQVGLTKNLSRRAIFITAGLLIAIVVAGTAVALWLYRSAALASG
                     EWHLRNVALALAAHARQGVLTADNTLRAMGEEYAREVAAGEFSEQAFHARIRQSA"
BASE COUNT          431 a          733 c          783 g          397 t
ORIGIN      
        1 cgcctgcttt tcttcctcct gaccggtgga ggaggatggc gtgaggaacg caatgcctga
       61 attcttcaga tcaccggcac gaagtgaaat gccgtgctcc tgagcggtgg gcacgatctg
      121 cgcaacgaag cacccgctca ggcccagcgc cagcgcccac accgccgcgc tcgccaccgc
      181 gcagcgggat tgagcaccca gccgcttttt catgaactgt ctgatcactc gctgttgttt
      241 agcaaagaag aatagcggtt gtggcggtga gcgtctttga catagctcag tcgcattgga
      301 caccggtcga tgcaacggcc catgcgtgag caatttttgc aaatgtcgaa tctttttatt
      361 tgcgtgccgg gggtgatgcc catttccatt gaaagtacga ggaacgatcg ttagactttc
      421 gctcgagcgg cacacgaaag ctggcgcgtt tgtcccgagc gagcgcgtgt gccagtgtca
      481 ccgatgaccg gatacccaga ggaaattgtt ccatgtcgaa gacgaaacgc agcggcaaca
      541 aagcgcccga caccccgaaa agcgagcctc aagccatggc gggagatggc gcaacacggc
      601 tgaccgagga cgagcgccgc cgcatggtcg ccgaggccgc gtactaccgc gctctgcagc
      661 gcggatttgc cgcgggcggt gaagtggatg actggctggc ggccgaacgc gagatcaacg
      721 atcagttcgc agcggcggga aaccacatcg tcgcgcgtaa cgcggcgccg gcgcgcgcca
      781 agggtaaagc cgccagcgca cgcagcgctg ctcgttcagt gcagtagcac ttcggatacc
      841 ggccgccgcg gggtgtggcg ggttccgcta ccgcgcccga ttcgaaggag tacctgcgga
      901 tagccctgct gcccgcacag cgagcccaag gctgagcgca gctctttcac ctcgatcggc
      961 tggttgagaa acgacgcgtg caggccgttc gcggtgacgg taagcagcac gcgctgtagc
     1021 gcctggcccg cgttcagcca gtcgagcgcg gtatcgcgcg cggttcccag acatgcgagc
     1081 agcggagagc cggtcgccag cgcgcgatcg cgcgcggcca ctccgttacc gagatcgaac
     1141 gtccgtacca ggaaagcaac tgccggaatc gccacgccga gcaattgcct cgcgcctttc
     1201 gcgtgcgcag ggagcccgtc gttgtcgcgc ggcgaatgca gccagctcgc gagctcggcg
     1261 cggaaggcgc ggttttcgaa ctgagcgcga tcgcctgtcg cgatgagatc cgccaccatt
     1321 tccttggagc gcgtgctcgc gaagggcgca agccacgcgc cctggatgcg ggcgacttcg
     1381 cacatttgcg cctgcagctc gccgctcacc gcctccaccg aataacgccc gcggtccgtc
     1441 atgcggcgcg gaatcgcgtg aaagagctcg gaccactcgc ttttctgccc ggagcccgcc
     1501 tgcagcgcga cccgcgcaac gacgcccggg cggtgtgcat ccgggaacgg ccgggcttcg
     1561 gaggcgcagc cgaaataatg catggcggtg cgcaggttga agagcgcagc gccgcagctc
     1621 atgatgagct cgcggtcctg cgggtcggcg atgggcagcg agcgatcgta gtcggcgagc
     1681 acctccagcg tcgctccccg gatgcggaat cgccacggct gcgtattgtg cgtggatggc
     1741 gccagtatcg cgtaacgcag cagaaactcg agctgccgct cgggggcctc tccggaagga
     1801 aaatcctctt cgggaatttt ccaagcgtcg agcggagcgt tgtacggcac ggtctgcatg
     1861 cggcgagcaa ggaatttagc cagtcgccac tgtagtcgtt cgctcgtcgg gcgagcttga
     1921 tgtgcgtcaa gcttggcgca agcctcggga tgttcctcag tgggacgacc ataagagccc
     1981 cgcaagggtt tgaccgggga gaccctgttt cagacggccg ctataatcag ggctctcggg
     2041 gtgcggaatg caagtggggc tcacaaagaa cctgtcgcgg cgggcgatct tcatcacggc
     2101 cggcctgctg atcgcgatcg tcgtcgccgg caccgccgtc gcgctctggc tctatcgaag
     2161 cgccgcgctc gcgagcggcg agtggcacct gaggaatgtc gcgctcgcgc tcgccgcgca
     2221 tgcgcgccaa ggcgtgctga ccgccgataa tacgctgcgg gcgatgggcg aagagtatgc
     2281 gcgggaagtg gccgccggcg agttttcgga gcaggcgttc catgctcgca tacgccagag
     2341 tgca
//
LOCUS       DASURG010000330         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_977623, whole genome shotgun sequence.
ACCESSION   DASURG010000330 DASURG010000000
VERSION     DASURG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977623"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VFY80_05785"
     CDS             <1..1305
                     /locus_tag="VFY80_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6007452.1"
                     /translation="TMTLAFGRVYGGSTVVYTGTSLIAPQRVIEQWNVPGLTHGDVAA
                     RSRKYMAQNNVHFLDDTLINDNNRLFVEGARKAGYHAEQFPLNLKGCLGSSLCNLGCP
                     NAAKQGTHRVQLPAAERQGVEVITRAEVQRIEGRALRVRVHQVQPGEKGAPSEWQPGE
                     YLVAADTVVLAGGSIGSTALLLRSGLQKQLPRLGHGFTCHPAQILVAEHAQRITNDVG
                     HPKSFFVDRAAEEGYVLETCMYFPFITAKNMTGFGEAHSRFMRAFPRLQMILVLACDR
                     AVEQNRITVDRTGKPVVHYDFTPEVVRSLVAATRASARIFFAAGAVRVHASVAQPPLL
                     EQTDAHRIDARIQERWFAAGKTSISAAHLMGGCGMGRDRRDSVTDAWGRVHGMPWLRV
                     ADSALFPDALQINPYLTVMSLADRTAQAIKQDLRAGMAAAIA"
     gene            complement(1306..1710)
                     /locus_tag="VFY80_05790"
     CDS             complement(1306..1710)
                     /locus_tag="VFY80_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007453.1"
                     /translation="MSDQLAHKREERRKTDVDYDEGPPEARVREEDLEPHSGLRYVAR
                     LFKVLAILLGIMLIAEIIIGLSQQGSAAIVTLMVEATRTIVFGGLLWGIGDICLMLIE
                     SNHDLRATRILVGRLNRNIEELAENPPPNLRT"
     gene            complement(1731..>2432)
                     /locus_tag="VFY80_05795"
     CDS             complement(1731..>2432)
                     /locus_tag="VFY80_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007454.1"
                     /translation="PRTPGVWNVLVALRGAVRSVPDLSVLTLVPLSEKRGGRIGSYMI
                     GSWPYERGGRPRSPAYAPPRGLVQVTPQNLDLQVSDHFRLRDFVTKGQQNVWPKYVAM
                     SPRLLDKLELTIQELNASGVPVQHVGTISGFRTPSYNAGGGDTSGRGALSRHMYGDAM
                     DWYVDNNRDGSMDDLNGDGRVNKEDGRVIGLAAERVEKKYPELVGGIGLYSPTGAHNG
                     FVHIDTRGYRARWGW"
BASE COUNT          439 a          772 c          767 g          454 t
ORIGIN      
        1 acgatgacgc tggcctttgg gcgtgtgtat ggcggctcga ccgtggtata cacgggcaca
       61 tcgctgattg cgccgcagcg tgtgatcgag caatggaacg tacccgggct gacccatggc
      121 gatgtcgccg cacgctcgcg gaagtacatg gcgcagaaca acgtgcattt cctggatgac
      181 acgctgatca acgacaacaa ccgcttgttt gtcgaaggcg cgcgcaaagc cggttatcat
      241 gccgagcagt tcccgctcaa tctgaaaggc tgtctgggca gcagcctgtg caacctgggc
      301 tgtcccaacg cagccaagca gggcacgcat cgcgtgcagc tgccggcggc cgagcgtcag
      361 ggcgtggaag tcattacgcg cgcggaagtg cagcgcattg aaggccgcgc attgcgtgtg
      421 cgcgtgcacc aggtgcaacc gggtgagaag ggcgccccct ccgaatggca gccgggcgaa
      481 tacctggtgg cagccgacac cgtggtattg gcaggcggtt cgatcggcag cacggcgctg
      541 ctgctgcgat cgggcttgca gaaacagctg ccgcgcctcg gccacggttt tacctgtcat
      601 cccgcacaga tactcgtcgc ggagcacgcg cagcgcatca ccaacgatgt cggtcatccg
      661 aaaagcttct tcgtggaccg cgcggccgag gaaggctacg tgctggagac gtgcatgtac
      721 tttccgttca tcacggccaa gaacatgaca ggtttcggcg aggcgcacag ccggttcatg
      781 cgcgcgttcc cgcggctgca aatgatcctg gtactggcct gcgatcgcgc ggtcgagcag
      841 aatcgcatca cggtggatcg taccggcaaa ccggtcgtgc attatgactt tacgcctgaa
      901 gtcgtgcgct cgctggtggc ggcaacgcgc gcctcggcac gcatcttctt cgccgccgga
      961 gccgtgcgcg tgcatgcgtc cgtggcgcag ccgccgctac tggagcagac ggatgcgcac
     1021 cgcattgatg cacgcattca ggaacgctgg ttcgcggcgg gcaaaacatc gatttccgcg
     1081 gcccatttga tgggcggatg cggcatgggg cgcgaccgca gagacagcgt cacagacgcc
     1141 tggggccgcg tgcacggcat gccgtggctg cgcgtggctg actccgcgct tttcccggat
     1201 gccttgcaga tcaatcccta tctgacggtc atgtctcttg ccgaccggac tgcgcaggcc
     1261 atcaagcaag acctgcgcgc cggcatggcg gccgcgatcg cctgatcagg tgcggagatt
     1321 cggcggcgga ttttccgcca gctcctcgat attgcgattc aatcggccca cgaggatgcg
     1381 cgtcgcgcgc agatcgtgat tcgattcgat cagcatcagg caaatgtcgc cgatacccca
     1441 gagcagtccg ccgaatacga tggtgcgtgt tgcttccacc atgagagtca cgatggctgc
     1501 ggacccctgt tggctcaatc caatgatgat ctccgcgatc agcatgatgc cgagcagaat
     1561 ggccagcacc ttgaacaggc gcgccacata gcgcagcccg gaatgcggct cgagatcctc
     1621 ttcgcgtact cgtgcctcgg gtggaccttc gtcgtaatcg acgtctgtct tgcgccgttc
     1681 ttcgcgtttg tgcgctaatt gatcagacac tgttcctctc ccgcagaatt ctaccaaccc
     1741 caacgcgcgc gatagccgcg cgtgtcgatg tgcacgaagc cgttgtgcgc cccggtagga
     1801 ctgtacagac cgatgcctcc caccagctcc ggatacttct tctcgacgcg ctccgcggct
     1861 agtccgatga cgcgtccatc ttccttgttg acccggccat cgccgttcag atcatccatg
     1921 ctgccatcac gattgttgtc gacgtaccaa tccatggcat cgccgtacat gtggcgcgaa
     1981 agcgcgccgc gtccggatgt gtcgccgcca cccgcattgt aggaaggagt gcggaatccg
     2041 ctgatcgtgc cgacgtgctg caccggaacg ccgctggcat tcagctcctg gatggtcagc
     2101 tccagcttgt ccagcaggcg cggtgacatg gccacgtatt tgggccagac gttctgctga
     2161 cccttggtca cgaaatcacg cagccggaaa tgatcggaga cctgcagatc gaggttctgt
     2221 ggtgtcacct ggacgaggcc cctgggcggc gcgtacgccg gcgagcgggg tcggccaccg
     2281 cgctcatacg gccagctgcc gatcatgtag gagccaatgc gtccgccgcg cttttcggag
     2341 agcgggacga gcgtcagcac ggacaggtcg ggcacgctgc gcaccgcgcc gcgcagcgcg
     2401 accagcacgt tccacacacc cggcgtgcgc gg
//
LOCUS       DASURG010000331         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_569503, whole genome shotgun sequence.
ACCESSION   DASURG010000331 DASURG010000000
VERSION     DASURG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569503"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..185)
                     /locus_tag="VFY80_05800"
                     /pseudo
     CDS             complement(1..185)
                     /locus_tag="VFY80_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
     gene            complement(521..1138)
                     /gene="ampD"
                     /locus_tag="VFY80_05805"
     CDS             complement(521..1138)
                     /gene="ampD"
                     /locus_tag="VFY80_05805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX6007455.1"
                     /translation="MSSSRRGKRRGRPPEFHLSANGRVRGIRYIASPNCDERPAGVAV
                     ELVVIHAISLPPGEFGGPGIIELFTNRLNPAEHPYYATIAGSAVSSHFLIRRDGELIQ
                     FVPCGKRAWHAGVSSWCARERCNDFSVGIELEGADDVPFERPQYTVLAALTRALKAIY
                     PIAGIAGHSDIAPGRKTDPGPHFDWARYRALLETASFVDSRRAER"
     gene            complement(1116..2483)
                     /locus_tag="VFY80_05810"
     CDS             complement(1116..2483)
                     /locus_tag="VFY80_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6007456.1"
                     /translation="MKNQVLVVDDEADIRELLSMTLSRMGLETHCAANTVEAFALLQA
                     NNYDLCLTDMRLPDGDGLGVLDHVAKHYPSVPVAVITAHGSTENAVAALKAGAFDYLA
                     KPVSLNQLRTLVRSALKLPQPGQGRRPAESSDLPPNYPRLIGESPLMQSTRQMIDRLA
                     RTQAPIHVTGESGSGKELAARLVHLKGSRHEGPFVAVNCGAIPENLMESEFFGYKKGA
                     FTGAASDRDGFFQAADGGTLFLDEVADLPLQMQVKLLRAIQEKKVRKVGSTSEEQVDV
                     RIISATHRSLADEVKSGAFRQDLYYRLNVIELRMPSLREMREDIPALADAIVRRQSGA
                     KEPPRLTAAAIEELQRYDFPGNVRELENVLERALALSTDSEIDCNDLQLVPTESEKSV
                     ASDVSGLPLQERLDLVEKKAILDALAQTHYNRTAAAKLLGITFRALRYRMERLGINDE
                     LESKG"
     gene            complement(2486..>2763)
                     /locus_tag="VFY80_05815"
     CDS             complement(2486..>2763)
                     /locus_tag="VFY80_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6007457.1"
                     /translation="RHCRRQQASIRICVDVEGEGSTVKLDVVDDGPGVPTQLRNQLFE
                     PFFTTAAGGSGLGLYIAREVCEANGASLDYVESVSGAQFTVICRAGD"
BASE COUNT          476 a          888 c          874 g          525 t
ORIGIN      
        1 cgcatgagcg cgctcaccac agcttccgtc aggttcgcca ccaggtcccg cacacgggcc
       61 gaggccgccc cctgcccgcc gttcaccgcg atgaaggcct tcgtcatggc gatcgcgatc
      121 ttggagggct cgaacacgac cacgctgccg ttgcggcgga tcaccttgta ttggctgtag
      181 cgggacagtt cggcgggcgt cgcttcggga agggcgaaat tcgcggcgga aacgggcttc
      241 atcgtgccgg tggcaagctg catattctga ctccttgaat tgctgtctct tatggaactg
      301 cagggggcgc tcctccccct ccccgaacca ctatatattg tggttcttaa ccatcctctg
      361 aaccaattct agtagcgcgt tttcgggaac gtcaataggg cgggtgaagt atttttggcg
      421 ccgcccaaac gctgtgcggc aaagcgcggc aagccttgga aaagacgcgc gttggcgttt
      481 tggccacagc ggcttcgctg ctcaaaaagc gatcagcgaa tcagcgttcg gcacgcctgc
      541 tatcgacgaa ggacgccgtt tcgagcagcg cgcggtaacg cgcccagtcg aagtgcggcc
      601 cgggatccgt tttgcggccg ggcgcaatgt cggagtgacc cgcaatgccg gcgatcgggt
      661 atatcgcctt gagcgcccgg gtaagcgccg cgagcaccgt gtactgcggg cgctcgaaag
      721 gcacgtcgtc cgcaccctcg agttcgatgc cgacggagaa atcgttgcag cgctcgcgtg
      781 cgcaccagct cgagaccccg gcatgccagg cgcgcttccc gcagggaacg aactggatca
      841 actcgccatc gcggcggatg aggaagtgag acgaaaccgc cgatcccgca atcgtcgcgt
      901 aatagggatg ctccgcggga ttgaggcggt tcgtgaaaag ctcgatgatg ccgggcccgc
      961 cgaattcccc gggcggtagg ctgatcgcgt gaatgacaac cagctcgacg gctacgccgg
     1021 cgggccgctc gtcgcagttg ggcgaggcaa tgtagcggat gcctcttacc cgcccattcg
     1081 cgctgagatg gaactcggga ggcctgccgc gccgcttacc ccttcgactc gagctcatcg
     1141 ttgatgccga gccgttccat ccggtaacgc agcgcgcgga acgtgatgcc gagcagcttg
     1201 gctgcagcgg tgcggttgta gtgagtctga gccagcgcgt cgaggatcgc ttttttctcg
     1261 acaagatcga gccgctcctg cagcggcagc ccggagacgt cggacgcgac ggatttctcc
     1321 gattccgtgg ggacgagctg caggtcgttg cagtcgattt ccgagtcggt ggagagcgcg
     1381 agcgcgcgct cgaggacgtt ctccagctcg cggacgttcc ctgggaagtc gtagcgttgc
     1441 agttcctcga tggcggcggc tgtgagccgc ggcggctcct tcgcccccga ctggcgccgc
     1501 acgattgcgt ccgcaagcgc gggaatgtcc tcgcgcatct cgcgcaggct cggcatgcgg
     1561 agctcgatga cgttcaaccg atagtagagg tcctggcgga atgcgccgct tttcacctca
     1621 tccgcgaggc tgcggtgcgt agcactgatg atgcgcacat cgacctgctc ttcgctggtg
     1681 gacccgactt tgcgcacctt tttttcctgg atcgcgcgca agagcttcac ctgcatctgc
     1741 agcggcagat ccgcaacttc gtcgagaaag agcgtgccgc cgtccgccgc ctggaagaag
     1801 ccatcccggt cgctcgccgc accggtaaag gcacctttct tgtaaccgaa gaactcggat
     1861 tccatcaggt tctccggtat cgcgccgcag ttcaccgcga cgaatggacc ttcgtgccgc
     1921 gagcccttga gatgaacgag ccgggcggca agctccttgc cgcttcccga ctcgccggtc
     1981 acgtggatcg gcgcctgcgt gcgcgcgagc cggtcgatca tctgacgcgt cgactgcatg
     2041 agcggcgact cgccgatcag ccgtgggtaa ttgggcggca ggtcggagct ttccgccggg
     2101 cgccggccct gcccgggctg cggtagcttg agcgccgagc gtacgagcgt ccgcaactga
     2161 ttgagcgaaa ccggtttcgc gagatagtcg aacgcgcccg ccttcaacgc ggccaccgcg
     2221 ttttcggtgc tgccgtgcgc cgtgatgacc gcgaccggca cactcggata atgcttggcg
     2281 acgtgatcga gcacgccgag gccgtcaccg tccggaagcc gcatatcggt gagacacaga
     2341 tcgtagttgt tcgcctgcag cagtgcgaac gcttcgacgg tattcgcggc gcagtgcgtc
     2401 tcgagcccca tgcgactcaa ggtcatgctc aagagctccc tgatgtcggc ttcgtcatcg
     2461 acgacgagaa cctggttctt catccttaat ctcccgcccg gcagatcact gtaaactgcg
     2521 cgccgctcac gctttcaaca taatcaaggc tggcgccgtt cgcttcgcat acttcgcgcg
     2581 cgatgtagag gccgagcccg ctgccgcccg cggccgtggt gaagaacggc tcgaagagct
     2641 gattgcgaag ctgcgtcggc acgccgggcc cgtcatccac cacatccaat tttactgtac
     2701 tcccctcgcc ttccacgtcc acgcagatac ggatgctcgc ctgctggcgc ctgcagtggc
     2761 gca
//
LOCUS       DASURG010000332         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_365404, whole genome shotgun sequence.
ACCESSION   DASURG010000332 DASURG010000000
VERSION     DASURG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365404"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..554
                     /locus_tag="VFY80_05820"
     CDS             33..554
                     /locus_tag="VFY80_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX6007458.1"
                     /translation="MSVDLRRPLRGRAWKFGDSIETDAINPYYRYPTMEALKQHTMEA
                     YRPEFPKQVRPGDILVAGRNFGCGSSRPGLVLREVGIVAIIAESVARLFLRNSIARAI
                     PIFIAPGVTSIVNDGETLEIDYPNSVVRNAATGASVELRKFPPLIEEIFTAGGLPEYA
                     YQRYVRETTTRGA"
     gene            551..1528
                     /locus_tag="VFY80_05825"
     CDS             551..1528
                     /locus_tag="VFY80_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007459.1"
                     /translation="MKAGAQFAAACLVVVAAAANVARAQEAAYPSKPVRFVVGQSPGG
                     ATDIVARLVADKMKDTLGQNVVVENRTGAAGSIAGSFVSKSPPDGYTVLVVSSSYSIN
                     PSVYSSLPFDPQKDLAPVSLLAEAPFLLVVHPSLPAKNVKDLIALAKTKPGVLTYGSG
                     GNGSSGHLAGALFELASGVKLTHVPYKGAGQALIDVVSGQITFMFASVLSSGPHVKQQ
                     RLRVLAVTSTKRSSGMPEVPTVAEAGLGSYGTTTWYGLLVPAGTPQAVIERLSTAAHR
                     TVMSPMVRERIMTDGAEPIGSTPAGFQEHLATEIAKWRKVVKSAGITVN"
     gene            1486..2961
                     /locus_tag="VFY80_05830"
     CDS             1486..2961
                     /locus_tag="VFY80_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6007460.1"
                     /translation="MAQSREERRNHRQLMVAQARPTERFARHAVDLRYEDLPPTLVEL
                     LKQCVLDTLGVAIAATTLAPEAPMVLDYVEELGGKEVATIWGYGRKAPPAWAVFVNGS
                     LGHMVDYDDVGAGGHVSIVTIPVALAVAEQLGGVTGREFLTAIAAGTDIHTRLNAGIK
                     IPDWTIAEGWFPTQLFGYISGTATAARLYGAGVETMEDAFGIAFTQLAGSRQMAVGRS
                     THLRSMQAGFSGQGAVTAAELARRGIVGSREVLEGRYGLFKTYVRTDPDWDAMLCGLG
                     HEFPILGLHGFKVWPACGYTRATNTAIRELREKHGIRPADVESITVIGGSGATQLLSE
                     PLERKRRPQLAIDGKFSIPFTSAVMMVKGNVTLRDYTNEGLRDPEVLAMADKVTYRID
                     PSSELPVGGYSSLSRPTVEIRTRDGGIYSCKPAGVPGDPDHPVSDDLLEAKFRDCVSF
                     SATPIPPENIDRAIALIRNLEDLSDVTEIVRLLVPARSTQR"
BASE COUNT          540 a          927 c          994 g          529 t
ORIGIN      
        1 cggcgaaatc gtgaatccca aagaggtgat ccatgtcggt tgatctgcgg cgccccctgc
       61 gcgggcgcgc ctggaagttc ggcgattcca ttgagacgga tgcgatcaat ccctactacc
      121 gctatccgac catggaagcg ctcaagcagc acacgatgga ggcgtatcgg ccggaatttc
      181 cgaagcaggt ccggcccggt gacattcttg tcgccggtcg gaacttcggt tgcggctcga
      241 gtcggcccgg tctggtgctg cgcgaggtgg gcattgtggc catcatcgct gaatccgtcg
      301 cgcgcctctt cttgcgcaac agcatcgcgc gcgccatacc gatcttcatt gcgccgggtg
      361 tgacttcgat cgtgaacgac ggcgagacgc tcgagatcga ttatccaaac agcgtagtgc
      421 gcaatgctgc aaccggggcg tctgtcgagc ttcggaagtt cccgccgctt attgaggaaa
      481 tcttcactgc gggcggactg ccggagtatg cctatcagcg ctacgtgcgc gagacaacga
      541 cgaggggagc atgaaagcag gagcgcaatt cgcggccgcg tgtcttgtgg tcgtcgctgc
      601 ggcggccaac gtcgcccgcg ctcaggaagc cgcctaccca tcgaagccgg tgcggttcgt
      661 ggtcgggcag tcgcccggcg gcgcgacgga catcgtcgca cggctcgtcg cggacaagat
      721 gaaggacact ttgggacaga acgtggtcgt ggagaaccgc acgggggcgg cggggtcgat
      781 cgcggggagt ttcgtctcga agagcccgcc ggacggctac acggtgctgg tcgtgtcgag
      841 cagctactcg atcaatccga gcgtctattc cagtcttccg ttcgacccgc agaaggatct
      901 cgcgcccgtg tcgctccttg ccgaagctcc ctttctgctc gtcgtgcatc cctcgctgcc
      961 ggccaagaac gtgaaggacc tgatcgcgct cgcgaaaacc aaacccggcg tactgacgta
     1021 cggctctggc ggcaatggca gctcaggtca cctcgccggt gcgcttttcg agttggcttc
     1081 aggcgtgaaa ctgacgcacg tgccctacaa gggcgcgggg caggcgctga tcgacgtcgt
     1141 gtcgggccag atcacgttca tgtttgcgag cgtgctctct tccggaccgc acgtgaagca
     1201 acagcggctg cgcgtgctcg cggtcacgag caccaagcgt tcgagcggta tgccggaagt
     1261 accgacggtc gcggaagccg gtctcggcag ctacggcacg acgacgtggt atggacttct
     1321 cgtgccagcg ggaacgccgc aggcggtgat cgagcggctg tctaccgccg cgcacaggac
     1381 ggtcatgtcg cccatggtgc gggagcggat catgaccgac ggcgccgaac ccatcgggag
     1441 cacgccggcg ggattccagg aacatcttgc gaccgaaatc gcgaagtggc gcaaagtcgt
     1501 gaagagcgcc ggaatcaccg tcaactgatg gtcgcgcagg cgaggccgac cgaacgcttt
     1561 gcccggcacg cggtcgatct gcgctacgag gatctgccgc cgacactcgt cgagctgctc
     1621 aagcagtgcg tgctcgacac gcttggggtc gcgattgcgg cgactacact cgcgcccgaa
     1681 gcgcccatgg tcctcgatta tgtcgaggag ctcggcggga aggaagtcgc gacgatctgg
     1741 ggctacggtc ggaaggcgcc tccggcgtgg gcggtgttcg tgaacggcag tctcggccac
     1801 atggtcgatt acgacgatgt gggcgccggc ggacacgtga gcatcgtcac aatcccggtc
     1861 gcgcttgccg ttgccgagca gctcggcggc gtcacagggc gcgaatttct cactgcgatt
     1921 gccgccggga ccgatatcca cacgcgcctc aacgccggca taaaaatccc ggactggacc
     1981 atcgccgaag gttggtttcc cacgcagctc ttcggctata tctcggggac ggcgacggcg
     2041 gcaagactct acggtgccgg cgtcgagacg atggaggacg ctttcggcat cgcgttcacg
     2101 cagcttgccg gcagccgcca gatggcggtc ggtcggtcga cgcatctgcg ctcgatgcag
     2161 gcgggcttct cggggcaggg cgcggtaacc gccgccgagc tcgcgcggcg cggcatcgtg
     2221 gggtcgagag aggtgctcga aggccgctat ggcctcttca agacctacgt gcgcacggat
     2281 cccgactggg atgccatgct ctgcgggctc gggcacgagt tcccgatact gggcttgcac
     2341 ggcttcaagg tgtggcccgc atgcggctac acgcgcgcga ccaacacggc gatccgggag
     2401 ctgcgcgaaa agcacggcat acggcccgcc gacgtcgagt cgatcaccgt catcggcggg
     2461 agcggcgcga cgcagcttct gtcggagccg ctcgagcgca agcggcgccc gcaactcgcg
     2521 atcgatggga agttcagcat tccgttcacc agcgcggtca tgatggtgaa gggtaacgtc
     2581 acgctgcgcg actacaccaa cgaaggtttg cgcgatcctg aagtgcttgc gatggccgac
     2641 aaagtgacct accgcatcga tccttcgagc gagcttccgg taggaggcta ctcttcgctg
     2701 tcacgaccca cggtagagat ccgcacgcgc gatggcggca tctactcgtg caagccggca
     2761 ggcgtgccgg gcgatcccga tcaccccgtg agcgacgact tgctcgaagc caaatttcgc
     2821 gactgcgtat cgttttctgc gacgccgatc ccacccgaga acatcgatcg cgcgatcgct
     2881 ctcataagga atctcgagga cctttcagac gtcacggaaa tcgtgcggct gctcgtgccc
     2941 gcgcgtagca cgcagcgtta gaggaggaat tatgcgaagt ttcatcgctg
//
LOCUS       DASURG010000333         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_365502, whole genome shotgun sequence.
ACCESSION   DASURG010000333 DASURG010000000
VERSION     DASURG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365502"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1590
                     /locus_tag="VFY80_05835"
     CDS             313..1590
                     /locus_tag="VFY80_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6007461.1"
                     /translation="MRQRTIKKGEAENSLNRRSFLELTAGASAAAFLCTMDAEAAALP
                     AAKITSIRVYEGPTVMPLQIPLLQSSMVVMIDTDIGVTGIGEGGTPDSLAPTAARLIG
                     QNPFEITRLWQDMYRSYHYPPGKERLHAIGALDLALWDLKGKVLEAPVYELLGGRVRN
                     YLETYTTGFRGTGSTLQERARECIEAGYRFYRFDAASVQGTNIYDARERVVRVAEDCA
                     AAREGVGSDGNYMVDFHQRFSYSDALRCCKMIEDTYPYFVEDPVEMNSFQQDIPKLRQ
                     MTSVPLAAGEEWGDRYDFIELVENHDLDFIRISLPNVGGITELIRVMAMCESHDVGIV
                     PHFTGPIATAAQAHALATFPFTVVFEYNYQTRPIPYLQENWITFNEGKLYTNDRPGLG
                     VEVDTSSLRLVATYTEANNRPMYYRPDGSPITW"
     gene            1723..2067
                     /locus_tag="VFY80_05840"
     CDS             1723..2067
                     /locus_tag="VFY80_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6007462.1"
                     /translation="MATYSAPNMFGALRFGDKLVREGDGVAAVFASGNRDEDVFGNPD
                     EFDLNRRKKDLLTFGRGPHMCAGAPLARLLLEVAIEGIVRRTRRIEVAGEITMTNWPE
                     YGPLAVPIRIAR"
     gene            2323..>2518
                     /locus_tag="VFY80_05845"
     CDS             2323..>2518
                     /locus_tag="VFY80_05845"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HEX6007463.1"
                     /translation="MSASRKTYRRRLSTLLGASKNEVRETDRWHDRILAGLDFGAVDN
                     IKSHAALTDAELARLLGMGEA"
BASE COUNT          495 a          829 c          784 g          410 t
ORIGIN      
        1 cacgcccacc actgcgacgg cgataaccgg ccgtagccac gtcacggttt ccgcggatcg
       61 cgagggaaat cgcatccatt tcaatctcgg cttggcgacg tgcctgggcg actgcgaaat
      121 gcctagcctt cgcacggtgc gggtgggtcg tcctgcaccg aatgcggctg tgaacagaca
      181 ggtggttcaa aattcctatc cgcttccgcg tgatatagtc gatcgcccca cagggcatgc
      241 ggccccggaa tcgggatcga gtcggatcaa gcgcaacgta tcacctcgga gctcgaacaa
      301 aggaagccac tgatgcgcca acgaacgatc aagaaaggcg aagcggaaaa ttccttgaat
      361 cgccgcagct tcctcgagct gacagccggc gcctcggccg ccgcatttct ctgcacgatg
      421 gacgccgaag cagcggcgct tcctgcagcg aagatcacgt cgattcgcgt ctacgagggg
      481 ccgaccgtga tgccgctgca gatcccgctg ctgcagagca gcatggtcgt gatgattgac
      541 accgacatcg gcgtcaccgg catcggcgag ggcggcacgc ccgactcgct cgcccccacg
      601 gccgcgcgcc tgatcggcca gaatccgttc gagatcacgc gcctatggca ggacatgtac
      661 cgctcctacc attacccgcc cggcaaggag cgcctgcacg cgatcggcgc gctggacctc
      721 gcgctgtggg atctcaaggg caaggtgctc gaagcgccgg tctacgagtt gctcggcggc
      781 cgagtccgca actatctcga gacctacact acgggattcc gcgggacggg cagcactctt
      841 caggagcgcg cccgcgagtg catcgaggcg ggctatcgct tctaccgctt cgacgcggcg
      901 agcgttcagg gcacgaacat ctacgacgcg cgcgagcgcg tagtgcgcgt ggccgaggat
      961 tgcgccgcgg cccgcgaagg cgtcggctcg gacggcaact acatggtcga cttccaccag
     1021 cgcttctcgt actccgatgc gctgcgctgc tgcaagatga tcgaggacac gtatccgtac
     1081 ttcgtcgaag acccggtgga gatgaactcg ttccagcagg acatcccgaa gctgcggcaa
     1141 atgacgagcg tgccgctcgc ggcaggcgaa gaatggggcg accgctacga cttcatcgag
     1201 ctcgtcgaaa atcacgacct cgacttcatc cggatctcgc ttccgaacgt tggcggcatc
     1261 acggagctga tccgcgtgat ggcgatgtgc gagtcccacg acgtcggcat cgtgccgcac
     1321 ttcacggggc cgatcgcgac agcggcccag gcgcatgcgc tcgctacctt ccccttcacc
     1381 gtcgtgttcg aatacaacta ccagacccgg cccatcccgt atctgcagga aaactggatc
     1441 accttcaacg agggcaagct ctacacgaac gaccgcccgg gtctcggcgt cgaggtcgat
     1501 acgagctcgc tgcggctcgt cgcgacgtac accgaggcga acaatcgccc gatgtactac
     1561 cgaccggacg gttcgccgat cacctggtga tgttcggcgc cgcgatcgaa gcaggcacgt
     1621 aaagcggttc aatccgagcg cagcgtcact ccgaaaacgc gcgcaaggtt ccccgagacg
     1681 cgctcggagt agagccattc catcgtcaac aggtgcgaca ccgtggcgac gtactcggca
     1741 ccgaacatgt tcggagccct gcggttcggc gacaagctcg tgcgcgaagg cgacggcgtg
     1801 gcggcggtct tcgcgtcggg caatcgagac gaggacgtct tcgggaatcc ggacgagttc
     1861 gacttgaacc gccgcaaaaa ggacttgctg acgttcgggc gcggcccgca catgtgcgcc
     1921 ggcgcgccgc tcgcccgctt gctgctcgag gtcgcgatcg agggcatcgt gcggcgcacg
     1981 cggcgcatcg aggtcgccgg cgagatcacg atgacgaact ggcccgagta cgggccgttg
     2041 gccgtgccga ttagaatcgc gcggtagtgc cgttatcgcg caccccaagt tcgccgcgcc
     2101 gtccctccgg ctcgccgtcc agctcgctgc acaagccgct tgaaatacac ctgggtcatg
     2161 cgaaaacgaa gagcttctcg catacgtcga gagccgtcgg ggctaccgcg cgcgctagga
     2221 aaaatgaatc cattcgatcc gccgaggcgg atcaagacca ccgccgcatt accgctcaga
     2281 accctcgccg catggctcga atgagcgtat agtagcgctc aaatgagcgc gagccgaaaa
     2341 acttaccgcc gccgcctatc cacgctgctc ggcgcatcga agaacgaggt gcgtgagacg
     2401 gatcgctggc acgaccgcat cctcgccggg ctcgatttcg gcgccgtcga caacatcaaa
     2461 agccatgcgg cgctgacgga tgccgagctc gcgcgcctgc ttggcatggg cgaggcga
//
LOCUS       DASURG010000334         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_365591, whole genome shotgun sequence.
ACCESSION   DASURG010000334 DASURG010000000
VERSION     DASURG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365591"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..511
                     /gene="moaE"
                     /locus_tag="VFY80_05850"
     CDS             53..511
                     /gene="moaE"
                     /locus_tag="VFY80_05850"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEX6007464.1"
                     /translation="MTVRIQTEDFEIGAEIAALRRANPAVGAVASFVGIVRDVNEGAA
                     IEAMTLEHYPGMTERSIDEIITQARGRWNIFDVLVIHRVGTLKPLDQIVLVIVTSGHR
                     GHAFAACEFIMDYLKTRAPFWKKEQTSAGARWVDARASDDMAAERWRLKG"
     gene            complement(548..1318)
                     /locus_tag="VFY80_05855"
     CDS             complement(548..1318)
                     /locus_tag="VFY80_05855"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6007465.1"
                     /translation="MTTGAHSGRPAAFVTGASQGIGAAIAVHLARHGYDVAVSSTRPD
                     KLGSTLSAISAAGARGIPIALDLCEQADIERAAAAALGAFGHIDVLVNNGGVPLRKLA
                     LEVTPEEWDSVLAPNLTGTFFLTQQIARHWVERARPGCVVNITSTHGIVGRAERSTYG
                     ISKAGLMHMTRMLALEWAPHGIRVNAIAPGRVESGSPARAATAANPDYLKRVLEGIPL
                     RRFCSVEEVAEAVRYLASPEARYITGHTLVMDGGLTAA"
     gene            complement(1315..>2096)
                     /locus_tag="VFY80_05860"
     CDS             complement(1315..>2096)
                     /locus_tag="VFY80_05860"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6007466.1"
                     /translation="LHVGESVSLSDAEQRKLLEFAIANVNGRVPVIAHASDSGTGIAV
                     GRARHAQDVGAAAVVATTPYYWTPPAGMVLEHFARIGTAVRIPFLVLYTPDEMPGTKL
                     APDLILKLIDRLPNFAGVVDASLDWQFMVNVASAAIRVRPDFQMLSGTEYMVSAGALG
                     ASSMFTSLAGIAPNLVRQLFERCRTEQYFEARPWQESVSALRQIVKPRGFSGLKSALR
                     VLGRDCGDPRPPNAPLGRDAYAAFAAELEASAELRAEPRGW"
BASE COUNT          356 a          668 c          721 g          351 t
ORIGIN      
        1 atgacggaga tgaggtggcg ttttttcctc cggtgactgg aggctgaagg cgatgactgt
       61 ccggatccag acggaagact tcgagatcgg cgccgaaatc gcggcactgc ggcgcgcgaa
      121 tcccgcggtc ggtgcggtgg cgagcttcgt cggcatcgtc cgcgacgtga acgaaggcgc
      181 ggcgatcgag gcgatgacgc tcgagcacta tcccggcatg accgagcgct cgatcgacga
      241 gatcatcacc caggcgcgcg ggcgctggaa tatctttgac gttttggtca tccatcgcgt
      301 cggcacattg aagccgctcg accagatcgt gctcgtcatc gtcacgagcg gacatcgcgg
      361 ccacgcattc gcggcgtgcg agttcatcat ggattatctc aagacgcgcg cgccgttctg
      421 gaaaaaagag cagacgtcgg cgggcgctcg atgggtcgat gcgcgcgcga gcgacgatat
      481 ggcagcggag cgctggcggc tgaagggctg actcacgtcg tgcaccacgc tgagcatttc
      541 gcgcgcttca ggcggcggtg aggccgccgt ccatgacgag cgtgtgtccg gtgatgtagc
      601 gcgcttcggg acttgcgagg taacgcactg cttcggcgac ttcctcgaca ctgcagaagc
      661 ggcgcagcgg tatgccctcc agcacccgct tgagatagtc cgggttcgcc gcggtggcgg
      721 cccgcgcggg tgacccgctt tccacgcgtc ccggtgcgat cgcgttgacg cgtatgccgt
      781 gcggcgccca ttccagcgcg agcatcctgg tcatatgcat caggccggcc ttcgagatgc
      841 cgtaggttga ccgttccgca cgcccgacga tgccgtgcgt ggaggtgatg ttcaccacgc
      901 atccaggccg ggcgcgctcc acccagtgac gggcgatctg ctgcgtcagg aaaaacgtcc
      961 cggtgagatt cggcgcgagc acgctgtccc actcttccgg cgtcacctcg agcgcgagtt
     1021 tgcgcagagg cacgccgcca ttgttgacca gcacgtcgat gtggccgaat gcccccaacg
     1081 ctgcggccgc ggcgcgctcg atatcggctt gctcgcacag atcgagcgcg atgggtatgc
     1141 cgcgagcccc cgcggcagat atggccgaga gcgtggatcc aagcttgtcg ggtcgcgtgg
     1201 aggagaccgc aacatcgtag ccatggcgtg cgagatggac cgcgatcgcg gcgccgatcc
     1261 cttgcgatgc accggtgacg aacgctgcgg gacgcccgga gtgagcgccg gtggtcacca
     1321 gccgcgcggt tccgcccgca actcggcgct cgcttcgagc tctgctgcaa acgcggcata
     1381 cgcgtcacgg ccgagcgggg cgttgggcgg tcgtgggtcg ccgcagtcgc gtcccagaac
     1441 gcgcagagct gatttgagtc cggagaaacc gcgcggcttc acgatctggc gcaaggccga
     1501 gacgctttcc tgccacgggc gcgcttcgaa gtactgctcc gtgcggcagc gctcgaagag
     1561 ctgccgcacg aggttgggtg cgatacctgc aagggatgtg aacatgctgc tcgcgcccag
     1621 cgcgccggcc gacaccatgt attccgtgcc ggacagcatc tggaaatccg ggcgcacgcg
     1681 gatcgcggcc gaggcgacgt tcaccatgaa ctgccagtcg aggctcgcat ccacgacgcc
     1741 cgcgaagttc ggcagacggt cgatcagctt gagtataaga tccggagcga gttttgttcc
     1801 gggcatctcg tccggcgtgt acagcaccag gaacgggata cgcaccgccg tgccgatccg
     1861 ggcgaagtgc tcgagcacca tcccggccgg cggcgtccag tagtacggag tcgtcgcaac
     1921 cacggccgcc gcgccgacat cttgagcgtg gcgggcgcgg ccaactgcga taccggtgcc
     1981 ggagtcgctg gcgtgggcaa tgacgggcac ccggccgttc acgttcgcaa tcgcgaactc
     2041 gagcagcttg cgctgctcgg catcggagag actcaccgat tcgccgacgt gcagcg
//
LOCUS       DASURG010000335         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1080198, whole genome shotgun sequence.
ACCESSION   DASURG010000335 DASURG010000000
VERSION     DASURG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080198"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFY80_05865"
     CDS             <1..237
                     /locus_tag="VFY80_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6007467.1"
                     /translation="LEVLEQRLKGRSQDGQDAIARRLAAAREEIGHFREFDYVIINKD
                     FNRAALELASIVRAERLRLPRQLARHADLINGMQ"
     gene            253..459
                     /gene="rpoZ"
                     /locus_tag="VFY80_05870"
     CDS             253..459
                     /gene="rpoZ"
                     /locus_tag="VFY80_05870"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX6007468.1"
                     /translation="MARITVDDCLKQIPNRFEMSLAATYRARQITNGAQSMLEANRDK
                     PTVIALRELAAGKYGPEILNKGQT"
     gene            469..2583
                     /locus_tag="VFY80_05875"
     CDS             469..2583
                     /locus_tag="VFY80_05875"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX6007469.1"
                     /translation="MGEAAALFKEWSGYLKPEDISQLQTAYHFSEAAHQGQFRQSGEP
                     YITHPLAVANILAQWHLDSQALTAALLHDVMEDAAVTKTEISRTFGKPVAELVDGVSK
                     LDRIEFETQERAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLEAVEPRKRRRIAR
                     ETLEIYAPIANRLGLNSIYQELEDLAFRHLYPERHRVLAKAVKAARGNRREVVGKVLE
                     SIKKRLKDNRIDAQVHGREKHLYSIYRKMREKRLSFAQVLDVFGFRIVVKDVQACYLA
                     LGALHGLFKPIPGKFKDYIAIPKANGYQSLHTTLFGPFGTPLEAQIRTSEMHKIAEAG
                     VASHWLYKSSDTSLSDLQQKTHQWLQSLLQMQSESGDSAEFLEHLKVDLFPDEVYVFT
                     PRGQIMAMPRGATAVDFAYAVHTDIGNRCVAVKINQELMPLRTELKNGDRVEVITAAH
                     AKPNPLWLNFVVTGKSRSAIRHFLKTMHFQESVQLGERLLNQALANFKAHPSTITELQ
                     WEKLVRESNAKSRQEILSDIGLGKRLSAVVARKLLALGEAPLVETAAASSVVIHGSEG
                     MAVQFARCCKPIPGDPIMGMISKGQGMVIHTHDCPSVARTRIDPERLLDVAWAPETRK
                     LFEVSIKLVVANRRGVLAHVAAEIAEAGSNIQNVSVTPGDDGTYTDMLFTLQVSNRLH
                     LARILRALRRIPEVVRINRVKG"
     gene            complement(2564..>2753)
                     /locus_tag="VFY80_05880"
     CDS             complement(2564..>2753)
                     /locus_tag="VFY80_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007470.1"
                     /translation="RPGGASIPAIALTGYAGGDARDRAVSSGFQAHLAKPFDTHELLN
                     LIGTLTDKAAKRLSPSHD"
BASE COUNT          557 a          913 c          839 g          444 t
ORIGIN      
        1 ctcgaggttc tcgagcagcg gctgaaaggg cgcagccagg acgggcagga tgcgatcgcg
       61 cggcggctcg cggcggcacg cgaggaaatc ggccatttcc gcgaattcga ctatgttatt
      121 atcaacaaag acttcaaccg cgcggcgctg gaactggcga gcatcgtccg ggccgaacgg
      181 ctgagactcc cccgccagct cgcccgccac gcggacctga tcaacggcat gcaatgaagg
      241 aagcgatatc gtatggcccg aattaccgta gatgactgtc tcaaacagat cccgaaccgc
      301 ttcgagatgt cgctcgccgc gacctaccgc gcgcgccaga tcaccaacgg cgcgcaatcg
      361 atgctcgaag cgaaccgtga caagcccacc gtcattgcgc tgcgagagct ggccgccggc
      421 aaatacggcc ctgaaattct gaacaagggc cagacgtagc caccatgcat gggtgaagcc
      481 gccgcactgt tcaaggagtg gtccggctac ctcaagccgg aggacatctc gcagctccag
      541 acggcctacc acttcagcga ggcggcgcac cagggacaat tccggcagtc cggcgaacct
      601 tacatcaccc acccgctcgc cgtcgccaac atcctggcgc agtggcatct cgactcgcag
      661 gcgctcactg ccgcgctcct ccatgatgtg atggaggacg ccgccgtcac caagaccgag
      721 atttcgcgca cgttcggcaa gccggtggcg gagctggtgg acggggtctc caagctcgat
      781 cgcatcgaat tcgaaacgca ggagcgcgcg caggccgaga acttccgcaa gatgctgctc
      841 gcgatggcgc gcgatgtacg cgtcatcctg atcaagctcg cggaccggct tcacaacatg
      901 cgcacgctcg aagccgtcga gccgcgcaag cgccggcgca tcgcccgcga gacgctggag
      961 atctacgccc ccatcgccaa ccggctgggg ctcaacagca tctaccagga actcgaagac
     1021 ctcgccttca ggcatctcta tcccgaacgc caccgcgtgc tcgccaaggc tgtaaaggcc
     1081 gcgcggggaa accgacggga agtcgtcggc aaggtgctgg aatcaatcaa gaagcggctc
     1141 aaggacaacc ggatcgacgc gcaggtgcac gggcgcgaga agcacctcta ttcgatctat
     1201 cgcaagatgc gcgagaagcg tctctcgttc gcgcaggtgc tcgatgtttt cggcttccgc
     1261 atcgtcgtga aagacgtgca ggcctgctat ctcgcgctcg gcgccctgca cggactcttc
     1321 aagccgatcc ccgggaagtt caaggactac atcgccattc ccaaggcgaa cggttaccag
     1381 tcgctgcaca cgacgctctt cgggcccttc ggcacgccgc tcgaggcgca gattcgcacc
     1441 agcgagatgc acaagatcgc ggaagcgggc gtcgcgtctc actggctcta caagagctcc
     1501 gacacgtcgc tctccgatct tcagcaaaag acccaccagt ggctgcagag cctgctgcag
     1561 atgcagtcgg aatccggcga ctcggcggaa tttctcgagc acctcaaggt cgatctcttt
     1621 cccgacgaag tgtatgtctt cacgccgcgc ggccagatca tggccatgcc gcggggcgca
     1681 accgcggtgg acttcgccta tgctgtccac accgacatcg gcaaccgctg tgtcgcggtg
     1741 aagatcaatc aggagctgat gccgctccgc acggaactca agaacggcga ccgggtggag
     1801 gtcattacgg cggcgcacgc caagccgaac ccgctgtggc tcaacttcgt ggtgacggga
     1861 aagtcgcgct ccgcgatccg gcatttcctg aagaccatgc atttccagga gtcggttcag
     1921 ctcggtgagc ggctcctcaa tcaggcgctc gccaacttca aggcgcaccc gtccaccatc
     1981 acggagctgc agtgggagaa gctcgtgcgc gagtcgaatg cgaagtcgcg gcaggaaatc
     2041 ctgtccgaca tcggtctcgg caagcggctg agcgccgtcg tcgcacggaa gctgctcgcg
     2101 ctcggagagg ccccgctcgt cgagacggcg gcggcgagct cggtcgtcat ccacggctcg
     2161 gaaggcatgg ccgtgcagtt cgcacgctgc tgcaagccca ttcccggcga ccccatcatg
     2221 ggcatgatca gcaagggcca ggggatggtg atacacaccc acgactgccc atccgtcgca
     2281 cgcacgcgga tcgatcccga gcggcttctc gacgtggcat gggcgcccga aacacgcaag
     2341 ctcttcgagg tgagcatcaa actcgtggta gccaaccgcc gcggcgtgct ggcgcacgtc
     2401 gcagccgaaa tcgcagaagc cggatcgaac attcagaacg tgtcggtcac ccccggcgac
     2461 gacggcacct ataccgacat gctgttcacg ctgcaggtgt cgaaccgcct gcacctcgca
     2521 cggatcctcc gcgcgctgcg ccgcataccg gaagtcgtcc ggatcaatcg tgtgaagggc
     2581 tgagtcgttt cgcggctttg tccgtgaggg tcccgatcag attgagcagc tcgtgcgtgt
     2641 cgaacggttt cgcaagatgc gcctgaaagc cgcttgagac cgcccgatcg cgcgcgtccc
     2701 cgccggcata accggtgagt gcgatcgccg ggatcgatgc gccgcctgga cgc
//
LOCUS       DASURG010000336         6831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_672567, whole genome shotgun sequence.
ACCESSION   DASURG010000336 DASURG010000000
VERSION     DASURG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672567"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFY80_05885"
     CDS             <1..611
                     /locus_tag="VFY80_05885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX6007471.1"
                     /translation="IGLMKAVKRFDPERGVRLVSFAVHWIRAEIHEFILRNWRIVKVA
                     TTKAQRKLFFNLRSLKQNLGTMGQDEVRAVASQLGVKAQEVVEMETRLGGQDVALEPS
                     TTEEPDETYAPIAYLAAEDAEPGDLLEQEQTARLRGEGLEKALESLDPRSRRIIQARW
                     LNERESATLHELAEEFGVSAERIRQIEAKALTKMKDLISETA"
     gene            complement(676..1263)
                     /locus_tag="VFY80_05890"
     CDS             complement(676..1263)
                     /locus_tag="VFY80_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6007472.1"
                     /translation="MRRRAVLALLLLSAALGACDRAPQARFQLTDLTGAPFGKALALT
                     GHDGKPRTLDDFRGKVVVIFFGFLHCPDVCPTTLAELAQVAKALGPDAARLQVLFVTV
                     DPERDTPELLRHYVTSFHPDFLGLRGDGTATTQVAKEFKIFFHKQPQSGSGYTVDHSA
                     GTYILDQQGRLRLFAHYGAGAGAFLNDIRLLLQGA"
     gene            complement(1284..2177)
                     /gene="cyoE"
                     /locus_tag="VFY80_05895"
     CDS             complement(1284..2177)
                     /gene="cyoE"
                     /locus_tag="VFY80_05895"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX6007473.1"
                     /translation="MSSTLARPYTASRAYQFYLLTKPRVVSLIVFTAVIGMFLATPGM
                     VPPQALIAGTLGIALIAGAAAAVNCLVEQKIDAVMLRTRARPLPSGRLTSAQTLVFAG
                     AIGGAGLAILHQWVNDLTMWLTLATFIGYAVVYTVILKPMTPQNIVIGGASGAMPPVL
                     GWTAVTGEISADALLLFLIIFAWTPPHFWSLALYRKLDYAKAGVPMLPVTHGDRFTRL
                     HVLLYTIILYACSLAPFATRMSGLIYLVCAIALGAVFLAYAIRIYTAYSDNLARRTFR
                     YSIFYLTALFAALLVDHYLKL"
     gene            complement(2178..3170)
                     /locus_tag="VFY80_05900"
     CDS             complement(2178..3170)
                     /locus_tag="VFY80_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX6007474.1"
                     /translation="MALIFRRLVFFTACFAFVVIVVGGYVRLEDAGLGCPDWPGCYGQ
                     LVGVPVEAHEIARAEEAFSKPVDVGRARKEMFHRYIAGTLGVLILAIALIAWSGRAAL
                     RQSPALATALVVLVALQAVLGKWTVTMLLKPVIVTLHLLGGMATLALLAWLALRQVPV
                     RAHPETAPRGLGILAVVGLTVVFVQIALGGWVSTNYAALACIDFPTCHGEWLPQMDFR
                     HAFHLVRELGMTAAGAPLSHEALTAIQWTHRVGALATLVTAGALGLAVLGTANFRLYG
                     IVLLGIVLIQIGVGTANVLLRLPLVLAAAHNGGAALLLVALVVLNFALFRKPSR"
     gene            complement(3161..3742)
                     /locus_tag="VFY80_05905"
     CDS             complement(3161..3742)
                     /locus_tag="VFY80_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HEX6007475.1"
                     /translation="MSEESAKRKASRAPLWILIALCIAPIVASYIAYYGWPPSERVNY
                     GELIEPRPLTDALLRALDGTAFHWRDLHGKWVLTVIDSARCDAWCQEKLLYTRQVRLA
                     QGRDADRIERVWLLTDEATPDPALVAGHAGLHVVRASGGEFLAAFPAVRSRADHIYVV
                     DPLGNTMMRYPRAADPRRMLKDVSRLLRHSKWR"
     gene            complement(3717..4430)
                     /locus_tag="VFY80_05910"
     CDS             complement(3717..4430)
                     /locus_tag="VFY80_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX6007476.1"
                     /translation="MSAASRHARAWPALAALAGIALTVSLGLWQLGRGQEKRALAMQV
                     ERLAQEPPIAVSDEELSAAAVEQRRVEARGVFEPKYMVLIDNRIWHRQPGYHVVMPLR
                     LGSGGRYVLVNRGWVAADADRSRLPQVKTPLGTQVVSGRAIAPGRFLELSEEVTEGPV
                     WQNLTLERYRKAMPIAIQPFIIQQDNVPAFDDGLVRDWPAPDLGVEKHYGYAFQWFAL
                     SALILALYVIAHVRRKREA"
     gene            4490..4684
                     /locus_tag="VFY80_05915"
     CDS             4490..4684
                     /locus_tag="VFY80_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX6007477.1"
                     /translation="MKLIVLLFILFILGSLFSALYFLLKDRGQTDRVVKALTIRVLLS
                     IALFALLMLGFYFGIITDKL"
     gene            complement(4795..5646)
                     /locus_tag="VFY80_05920"
     CDS             complement(4795..5646)
                     /locus_tag="VFY80_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX6007478.1"
                     /translation="MLNQSARYFIPEPSYWPIFGSVALLSMALGAASWFNGWAPGKFF
                     VIAGLLILFFMMAGWFTTVAHESEGGRYNKQVDTSFRWGMSWFIFSEVMFFGAFFGAL
                     FYMRVISVPDLGSIESQQLIWPGFKAGWPVSGPGIKEHFSPMGAWGIPALNTLLLLSS
                     GVTVTWAHWGLLKNNRLQLIWGLVMTVALGATFLGFQIYEYAHAYSELNLKLTMGAYG
                     ATFFMLTGFHGFHVTIGTIMLIVILFRSVAGHFTADHHFGFEGVAWYWHFVDVVWLLL
                     FIFVYWL"
     gene            complement(5675..5890)
                     /locus_tag="VFY80_05925"
     CDS             complement(5675..5890)
                     /locus_tag="VFY80_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEX6007479.1"
                     /translation="MLMTEPKHTPRKATPLEVAKAVFWSFLGIRKRAAHERDAVTIKP
                     AQVIVAGIIGAIIFVLCLVMLVRFITS"
     gene            complement(5890..6429)
                     /locus_tag="VFY80_05930"
     CDS             complement(5890..6429)
                     /locus_tag="VFY80_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6007480.1"
                     /translation="MNAASGQNVVTLRKLIVIACAMFGFGFALVPFYKKICEVTGINN
                     VLKADTVTNTQIDASRWLTVEFDANVRRGLPWTFTPVEKQVRFHPGELVQVTFEIRND
                     APRPVTGQAIPSYGPQLAGRYFKKLECFCFTQQTLQPGEVRRMPVVFVIEKDLPQDIN
                     TVTLSYSFFEVEGTAGKAG"
     gene            complement(6426..6518)
                     /locus_tag="VFY80_05935"
     CDS             complement(6426..6518)
                     /locus_tag="VFY80_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HEX6007481.1"
                     /translation="MSNRARTALILGSIALAFFIGIIVRYWMWG"
     gene            complement(6532..>6831)
                     /locus_tag="VFY80_05940"
     CDS             complement(6532..>6831)
                     /locus_tag="VFY80_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6007482.1"
                     /translation="FWLSMIFFNITFFPMHFSGLAGMPRRIPDYALQFADFNMISSLG
                     AFGFGLSQLLFLIVLFRLRSGRKAEDKAWEGAEGLEWTLPSPAPYHTFETPPVVK"
BASE COUNT         1387 a         2177 c         2155 g         1112 t
ORIGIN      
        1 acatcgggct catgaaggcc gtgaagcgct tcgatcccga gcgcggcgtg cggctggttt
       61 cctttgcggt gcactggatc cgcgccgaga ttcacgagtt catcctgcgc aactggcgca
      121 tcgtcaaggt cgcgaccacc aaagcccagc gcaagctctt cttcaacctc cgcagcctca
      181 agcaaaacct cgggacgatg gggcaggacg aagtaagggc ggtggcgagt cagctgggcg
      241 tcaaggccca ggaagtggtt gaaatggaaa cgcggctcgg cgggcaggat gtggcgctcg
      301 agccgtccac gacggaagag cccgacgaga cttacgcacc gatcgcgtac ctcgctgcgg
      361 aagacgccga gcccggcgac ctcctcgaac aggagcagac cgctcgactg aggggcgaag
      421 gcctcgaaaa ggcgctcgaa agcctcgatc cgcgcagccg ccgcattatc caagcgcgct
      481 ggctaaacga gcgggaatcc gccacgttgc acgagcttgc cgaggaattc ggtgtgtccg
      541 cggagcgcat ccggcagatc gaagccaaag cgctgaccaa gatgaaggac ctcatttccg
      601 agacggcctg acgcgcgccg tcacgtaacg gcatttccaa gccctgccaa tgcagggctt
      661 tttgttgtac cggctttacg ccccttgcag caggagccgg atgtcgttta gaaaagcgcc
      721 cgcacctgcg ccgtagtggg cgaaaagccg cagccgtccc tgctgatcca ggatgtaggt
      781 ccccgccgag tgatccaccg tgtagccgct tccggattgc ggctgcttat ggaaaaaaat
      841 cttgaattcc ttcgccacct gcgtcgtggc cgtgccatcg ccccgcagtc ccagaaagtc
      901 cggatggaag gaggtgacgt agtggcgcag cagttccggc gtatcgcgct cggggtccac
      961 agtgacgaag agcacctgca atcgcgcagc atccgggccc aacgccttcg caacctgagc
     1021 cagctcggca agcgtcgtgg gacacacgtc cgggcagtgc agaaagccga aaaagatcac
     1081 caccaccttg ccgcggaaat cgtccagcgt gcgcggctta ccgtcatggc cggtgagggc
     1141 gagtgccttg ccgaacgggg cgccggtgag gtcggtcagc tgaaatcggg cttgcggggc
     1201 gcgatcgcac gcgccgaggg ccgcactcag gagcagcaac gccagtacgg cacgccgtct
     1261 catgacgcag cgttcatacg ttgctagagc ttgaggtagt gatcgaccag aagcgctgcg
     1321 aagagcgccg tcaagtaaaa aatggagtag cggaaggtgc gacgtgcgag gttgtcgctg
     1381 tacgcggtgt agatgcggat cgcatacgcg agaaacacgg cgccgagcgc aatggcgcag
     1441 acaaggtaga tcagcccgct catgcgcgtg gcgaacggcg cgagcgaaca cgcgtacagg
     1501 atgatcgtat agagcaggac gtgcagccgg gtgaagcggt ctccgtgtgt tacgggcagc
     1561 atcgggacac cggccttcgc ataatcgagc ttgcgataga gggcaagcga ccagaaatgc
     1621 ggcggcgtcc aggcgaagat gatgagaaag agcagcagtg cgtcagccga gatctcgccc
     1681 gtgactgcag tccaaccgag caccggaggc attgcgccgg acgcgcctcc gatcacgatg
     1741 ttctgcggcg tcatgggctt gaggatcacc gtatagacaa cggcatagcc gatgaacgtg
     1801 gcgagcgtaa gccacatcgt gaggtcgttc acccactggt gcagtatcgc gagccccgcc
     1861 ccgccgatgg cgcctgcaaa gacgagcgtc tgcgccgaag tgagccggcc gctcggcaga
     1921 ggccgcgccc gcgttctcag catcaccgcg tcgatcttct gctcgacgag gcaattcacc
     1981 gccgcggcag cgcccgcgat gagcgcgatg ccgagcgtgc cggcgatgag cgcctgcggc
     2041 ggcaccatgc ccggtgttgc aagaaacatc ccgatcactg cggtgaatac gatgagcgat
     2101 accacccgcg gcttcgtcag taggtaaaac tgataagcgc gcgaagcggt atacgggcgg
     2161 gcaagggtcg aggacatcta gcgggagggc ttccggaaaa gcgcgaagtt tagcaccacc
     2221 agcgcgacga gcaaaagcgc cgcgcccccg ttatgggcgg cggcgagaac cagtggcaga
     2281 cgcaagagca cattcgcggt tccgacgcca atctgaatca gcactatccc gagtaagacg
     2341 atgccgtaga ggcggaagtt cgcggttccg agcactgcaa gccctagcgc acctgcggtc
     2401 acgagcgtcg cgagcgcgcc gacccggtgt gtccactgaa tcgcggtgag cgcttcatgg
     2461 gagagcggcg cacccgcggc cgtcatcccc agctcgcgca cgaggtgaaa cgcgtggcga
     2521 aaatccatct gcggcagcca ctcgccatgg caggtgggaa agtcgatgca cgcgagcgcg
     2581 gcgtagttcg tcgaaaccca tccgccgagc gcgatctgca cgaagacgac ggtcaagccg
     2641 accacggcga gaatgcctag tccgcgcggt gccgtttccg gatgggcccg gactggcacc
     2701 tgacgcaggg caagccaggc gagcagcgca agcgtcgcca tgccgccgag aaggtgcaac
     2761 gtcacgatca ccggcttcag cagcatggtc acggtccact tgccgagcac cgcttgcagc
     2821 gccaccaata ccactaacgc cgtggcgagc gcgggcgact gccgcagtgc tgcgcggcca
     2881 ctccacgcga tgagtgcgat ggcaagtatg agcactccca gtgtgccggc aatatagcgg
     2941 tggaacatct ccttgcgcgc ccggccgacg tccaccggct tgctgaacgc ctcttccgca
     3001 cgtgcaatct cgtgtgcctc aactggtacg ccgacgagct gcccgtagca gccgggccag
     3061 tcggggcaac cgaggcccgc gtcctcgagc cgcacatagc cgccgaccac aatcacgacg
     3121 aacgcgaagc aggcggtgaa aaaaacgagc cggcgaaaaa tcagcgccat ttggagtgcc
     3181 tcaggaggcg ggacacgtcc ttcagcatac ggcgcggatc ggcggcgcgc gggtaacgca
     3241 tcattgtatt gccgagcgga tccacgacgt agatgtggtc ggcacgcgag cgcacggcgg
     3301 gaaacgccgc cagaaattcg ccgccggatg cgcggacgac gtgcagtccg gcgtgccccg
     3361 cgacgagcgc tggatcgggt gtcgcctcgt cggtgaggag ccagacacgc tctatgcggt
     3421 cggcgtcccg tccctgggcg agccgcacct ggcgcgtata gagcagcttt tcctggcacc
     3481 acgcgtcgca gcgcgcggaa tcgattaccg tgagcaccca cttcccgtgg agatcgcgcc
     3541 agtgaaacgc agtgccgtcg agcgcgcgca gcagcgcatc ggtcagcggt cgcggctcga
     3601 tcagctcgcc gtagttcacc cgctcggagg gcggccaccc gtaatacgcg atgtacgacg
     3661 ccacgatcgg cgcaatgcag agcgcgatca gaatccagag cggcgcgcgg ctcgccttac
     3721 gcttcgcgct ttcttcggac atgagcaatg acatagagcg cgagaatcag cgccgacaag
     3781 gcgaaccatt gaaatgcata tccatagtgc ttttccacgc cgagatcggg tgccggccag
     3841 tcgcgcacga ggccgtcatc gaacgccggg acgttgtcct gctggatgat gaacggctga
     3901 atcgcaatcg gcatcgcttt ccggtagcgc tcgagcgtca ggttctgcca caccggcccc
     3961 tccgtgactt cttccgacag ttcgagaaag cgcccgggcg cgatcgcgcg gccgctcaca
     4021 acctgcgtcc cgaggggcgt cttcacttgc ggcaggcggc tgcgatcggc gtccgctgcg
     4081 acccagccgc gattcacgag aacgtagcga ccgccgctgc ccagcctaag cggcatgacg
     4141 acatggtagc cgggctgccg gtgccagatc cggttgtcga tgagcaccat gtatttcggc
     4201 tcgaacacac cgcgcgcctc gacgcgccgc tgctcgacgg cagccgcgct cagctcctca
     4261 tccgagaccg cgatgggtgg ctcctgcgcc aggcgttcga cctgcatcgc gagcgcacgt
     4321 ttctcctgtc cccgtccgag ctgccacagc cccagcgaga cggtcagcgc aatgcccgcc
     4381 aacgcggcga gcgcgggcca cgcacgcgcg tgtcggctag ccgcgctcat ccgtggcagc
     4441 tgcagggggc gtgcgataca ctgcggcatc cccgcgccca cacctgccca tgaaactgat
     4501 cgtactgctg ttcatcctct tcatcctggg aagcctcttt tcggcgctct acttcctgct
     4561 caaggaccgc ggccagaccg accgggtcgt caaggcgctg acaatacggg tgctgctgtc
     4621 gatcgcgctc ttcgcgctgc tgatgctcgg cttctacttc ggcatcatca ccgacaagct
     4681 ctgaggaaaa aaaagccgca ctcgcgtgcg gcgcgtattc ccctaccggc cgggacctcc
     4741 ctttgttatt gcccctttgt cgttcgcttg tgattgttct tgggtgtttg ggccttatag
     4801 ccagtagacg aatatgaaga gcagcagcca cacgacgtcc acgaagtgcc aataccacgc
     4861 cacgccttcg aatccgaagt ggtgatcggc ggtaaagtgc ccggcgacgg aacggaacag
     4921 gatcacgatg agcatgatcg tgccgatcgt cacgtggaat ccgtgaaagc cggtcagcat
     4981 gaagaacgtc gccccgtatg cgcccatcgt gagcttgagg ttgagctcgc tgtaggcatg
     5041 cgcgtactca tagatctgga atccgaggaa cgttgcgccc agggcaaccg tcatgaccag
     5101 accccagatc aactgcagcc gattgttctt gagcagtccc cagtgcgccc acgtcaccgt
     5161 gacgcccgaa gaaaggagga gcagcgtgtt gagcgcgggg atgccccacg cgcccatcgg
     5221 gctgaaatgc tccttgatgc ccgggcccga cacgggccat cccgccttga atcccggcca
     5281 gatcaactgc tggctctcga tgctgcccag gtccggaacc gaaatcacgc gcatgtagaa
     5341 cagcgcgccg aaaaaggcgc cgaagaacat cacttccgag aagatgaacc agctcatgcc
     5401 ccagcggaag gacgtgtcca cctgcttgtt gtagcggccg ccctcgcttt cgtgcgcgac
     5461 ggtggtgaac catccggcca tcatgaagaa caggatgagc aggccggcga tcacgaaaaa
     5521 tttccccggc gcccagccgt tgaaccacga ggccgcgccg agcgccatgg agagcagcgc
     5581 aaccgacccg aagatcggcc agtacgaggg ctccggtatg aaatagcggg cggactggtt
     5641 cagcattgcg tttctccctc ttcctttggt ttgctcagct cgtgatgaag cgcaccagca
     5701 tgaccaggca cagaacgaaa atgatggcgc cgatgatacc cgccacgatc acttgggcgg
     5761 gcttgatcgt caccgcatcg cgctcgtgcg cggcgcgctt gcggatcccg agaaaggacc
     5821 agaataccgc cttggccact tccagcggcg tcgccttgcg cggcgtatgt tttggctcgg
     5881 tcatcagcat cagcccgcct ttcccgcggt cccctcgact tcgaagaagg agtaggacag
     5941 cgtcaccgtg ttgatgtcct ggggcaggtc tttctcgatc acgaacacga ccggcatgcg
     6001 gcgcacttca cccggctgca gcgtctgctg cgtgaagcag aagcactcca gcttcttgaa
     6061 atagcgtccc gcgagctgcg gaccgtagct cgggatcgcc tggccggtca ccgggcgcgg
     6121 ggcgtcgttg cgaatctcga atgtgacctg caccagctcg ccgggatgga agcgcacctg
     6181 cttctcgacc ggcgtgaagg tccacggcag cccgcgcctc acattggcgt cgaactccac
     6241 cgtgagccag cggctcgcgt cgatctgcgt gttggtgacc gtgtccgcct tcagcacgtt
     6301 gttgatgccc gtcacctcgc agatcttctt gtagaacggc acgagcgcga agccgaagcc
     6361 gaacatcgcg caggcgatca cgatgagctt gcgcagcgtg acaacgttct ggcccgacgc
     6421 cgcgttcatc cccacatcca gtaacggacg atgatgccga tgaaaaacgc gagcgcgatg
     6481 gagcccagta tcagcgccgt tcgtgcacgg ttgctcaagg cctgcacctc gctatttcac
     6541 gaccggtggc gtctcgaacg tgtgatacgg tgccggagag ggcagcgtcc actcgagccc
     6601 ctccgcgccc tcccaagcct tgtcctcggc tttccggccg ctgcgcagcc tgaagagcac
     6661 gataagaaag agaagttgcg agagtccgaa tccgaaggcg ccaagcgacg aaatcatgtt
     6721 gaagtccgcg aactgtagcg catagtcggg aatgcgccgc ggcatgccgg caagccccga
     6781 gaagtgcatc gggaaaaacg tgatgttgaa aaagatcatc gagagccaga a
//
LOCUS       DASURG010000337         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_59296, whole genome shotgun sequence.
ACCESSION   DASURG010000337 DASURG010000000
VERSION     DASURG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59296"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFY80_05945"
     CDS             complement(<1..221)
                     /locus_tag="VFY80_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007483.1"
                     /translation="MDFFFHPSSNKALDPLPTASHTWSAILVAGHRHGRPQNNPGESI
                     MRPTSATARFKPWVVAIALCFATVPQLWS"
     gene            220..867
                     /locus_tag="VFY80_05950"
     CDS             220..867
                     /locus_tag="VFY80_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HEX6007484.1"
                     /translation="MIDTMSGAYLDLAAWPRRNHFELFRGYDQPFFNVTADVRVSSTY
                     RLCRDAGHSFFLATLYQSLCAANRTEEFRLRLRVDGVWRYDRVHAGSTLLRPDGTFGF
                     GYFDAAPNFSEFQANGRAVIATVRAATELNPEDERDDLLHYSVLPWIHFTSFAHARTR
                     KHLDSVPKIVFGKRYERAGESWMPVSIEVHHALVDGLHVAAFLDAFQEGLDAFQI"
     gene            complement(887..2218)
                     /locus_tag="VFY80_05955"
     CDS             complement(887..2218)
                     /locus_tag="VFY80_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6007485.1"
                     /translation="MRYPPGPPGRPLIGNLPDFARDVLGFHERCREEFGDIARVRVGA
                     RTIYLVQHPELIDEVLVTNHRNFIKHSFFWRHVTAIFGRGLLTNEGESWLQQRRLMAP
                     AFHRDRIVSYGNVMVEYTQRALADWKPGELRNVHHDLMHLTLEIVAKVLFDADVGGDA
                     EVIGRAFDAVTDEIAARFRRPVFIPDWVPLPGNFRYRAGVRALDALVYRIIAEHRASG
                     AQGNDLLSMLMSVRDEEGSCMSDQQLRDEAITILLAGHETTALALSWTLYLLALHPDV
                     DARLQDELQQVLNGRTPTPADLPALRYTDHVIKEAMRIYPPAYGIGREAVAACELGGY
                     HVPAGTTIFMSAWVTHRDPRWFADPHTFNPDRWNDGLAERLPRHAYMPFGGGPRICIG
                     NSFAIMEATLLLASIAQRYRFVREDPIPVVPFPTITLRPSGGVQLRVHARA"
BASE COUNT          475 a          790 c          782 g          459 t
ORIGIN      
        1 tgactccata actgcggtac ggtggcgaag cacagtgcaa tggctacaac ccagggcttg
       61 aagcgtgcgg ttgctgaagt cggacgcata atggattctc cgggattatt ttgtgggcgg
      121 ccgtggcggt gcccggcgac gaggattgcg gaccatgtat ggctcgctgt cggcaaggga
      181 tcaagagcct tgttgcttga cggatgaaag aaaaaatcca tgattgacac catgagcggg
      241 gcctacctcg acctggccgc ctggcccagg cgcaatcact tcgaattgtt ccgcggctac
      301 gaccagcctt tcttcaatgt tactgcggac gtacgtgtct ccagcaccta ccggctttgc
      361 cgcgacgcgg ggcactcgtt ctttctcgcg accctctacc agtctctgtg cgccgcgaat
      421 cgcaccgagg aattccggct acggttacgc gtcgatgggg tgtggcggta cgacagagtt
      481 catgccggct caacgctgtt gcgcccggac ggcacgtttg gtttcgggta cttcgatgcc
      541 gcgcccaact tctcggagtt ccaggcgaac gggcgggcgg tgatcgctac cgtgcgcgca
      601 gctacggagc tgaacccgga ggacgagcgc gacgacctgc tccactactc ggtcctgccc
      661 tggattcatt tcacgagctt tgcgcatgcg cgcacacgca agcatctgga ctccgtgccg
      721 aagatcgtgt tcggcaaacg ctacgaaagg gccggcgagt catggatgcc ggtatcgatc
      781 gaggttcacc acgcgctcgt ggacgggctc cacgtggccg ccttcctgga tgcgtttcaa
      841 gaggggctgg acgcctttca gatctgacgc ggccgcaccg ttgcggtcac gcgcgtgcgt
      901 gcactctcag ttgcaccccg ccactcggcc gcaaggtaat cgtcgggaat ggcaccaccg
      961 gtatcggatc ttcgcgcaca aagcggtaac gctgggcgat gctcgccaac agcagtgttg
     1021 cttccattat cgcgaagctg ttgccgatgc agatgcgcgg gccgccgcca aagggcatgt
     1081 aggcgtgccg cggcaggcgc tcggccaggc catcgttcca gcgatcggga ttgaaggtgt
     1141 gcgggtccgc gaaccagcgc gggtcgcggt gcgtcaccca cgcactcatg aagatggtcg
     1201 tgcctgcggg cacgtggtaa ccacccagct cgcaagcagc gaccgcttcc cgtccgatgc
     1261 catacgccgg tgggtagatg cgcatcgctt ccttgatcac gtggtccgtg tagcgcaatg
     1321 cgggcaggtc cgcgggcgtg ggtgtccggc cgttcagcac ctgctgtagc tcatcctgca
     1381 aacgtgcatc gacgtccgga tgcagtgcca gcagatacaa ggtccacgac agcgccagtg
     1441 cggttgtttc gtgaccggcc agcagaatag tgatcgcctc atcgcgcagc tgttggtcgc
     1501 tcatgcaact gccttcttca tcacgcacgc tcatgagcat ggacaacagg tcgttcccct
     1561 gcgcgccact ggcgcgatgc tcggcaatga tgcggtagac cagcgcgtcg agtgcgcgca
     1621 cgcctgcgcg atagcggaaa ttgcccggca gcggcaccca atccggtatg aacacgggcc
     1681 ggcgaaagcg cgccgcaatc tcgtccgtca cggcgtcgaa cgcgcggcca atcacctcgg
     1741 cgtcaccacc aacatcagcg tcgaacaaaa ccttggccac gatctcgagg gtgagatgca
     1801 tgagatcgtg atgcacgttg cgcagctcgc ccggtttcca gtccgcgagc gcgcgctgcg
     1861 tgtattccac catgacattg ccataagaga caatgcggtc acgatgaaac gcgggcgcca
     1921 tcaggcgacg ctgctgcagc cacgactcgc cctcattggt cagcaggccg cggccgaaga
     1981 tcgcggtcac atggcgccag aagaacgaat gcttgatgaa gttgcggtga ttcgtcacca
     2041 gcacctcgtc aatcagctcg ggatgctgga ccaggtagat ggtgcgggct ccgacacgca
     2101 cccgcgcgat gtcaccgaac tcttcgcggc agcgctcgtg aaagccgagc acatcacgcg
     2161 cgaagtcggg cagatttccg atcagcggcc ggcctggcgg accgggtgga tacctcatgc
     2221 aggcaatata cggtaccgcg ccccgacgtt cgctcctcag cgtcctctgc tcagtctccg
     2281 tgtcctcgca gttccgggca cggagagtgc aagttgttcc tggagaatca gttgccccaa
     2341 aagggagtac catggccgag caggacaaga cctcaccatc cgagcatccg tcccgcccga
     2401 agaagggccg tatgaaacgc ctgaacacgc gacgcggacg ccgcaacctc gcgatcggca
     2461 tggccggcgc aaccatcggc ggtatcgcgg ccacgtccgg tgccga
//
LOCUS       DASURG010000338         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1387716, whole genome shotgun sequence.
ACCESSION   DASURG010000338 DASURG010000000
VERSION     DASURG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387716"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFY80_05960"
     CDS             complement(<1..291)
                     /locus_tag="VFY80_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6007486.1"
                     /translation="MPLNLPNLLTWLRIVLIPLFVGIFYFEKSWVSAPNQNLVATIIF
                     TAAAVTDWLDGWLARKLNQTSSFGEFLDPVADKLMVAAALITLVQLERVDAII"
     gene            complement(301..2103)
                     /gene="uvrC"
                     /locus_tag="VFY80_05965"
     CDS             complement(301..2103)
                     /gene="uvrC"
                     /locus_tag="VFY80_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX6007487.1"
                     /translation="MPDPAKIIANLPALPGVYRFLNEAGEVLYVGKALNLKKRVASYF
                     QKRTALAPRTQAMLSHVAGVEVTATRSEGEALLLESNLIKTLSPRYNVLFRDDKSYPY
                     LVVTGHPYPRLGFHRGALDKRNRYFGPFPNAGAVRESIQLLQKVFRLRTCEDTVFANR
                     SRPCLLHQIKRCTAPCVRLVSEADYAEDVRGAELFLKGKEQEVIDRLAGRMNAAAEAL
                     RYEDAALFRDQIGALRRVREKQFVSGESASDADVIACARVAGVTCVNLVMIRGGRHLG
                     DKNFFPRNAEGFTDAEVIEAFLAQHYVHHGAPANVVVGAAVDAAAFETLLSDRAHHKV
                     QVVTQPSSGRRAWLDMALKNAALGAAQTVGLQATQEARLSALQHALGLSETAQRIECF
                     DVSHTMGEATVASCVVYDRAGLQKGEYRRYNIEGVMPGDDYEAMRQVLVRRYRRIVAG
                     EGRMPDLVLIDGGKGQLAVAEAVCTELGLNDVMLVAVAKGEGRKPGLEQLFIAGRDAA
                     LALAPEHPALHLIQQIRDEAHRFAIQGHRARRAKARSSSPLETIAGVGAKRRQKLLTR
                     FGGLRGLMAASVDDMVQVEGISRTLAEKIYEELH"
     gene            complement(2158..2697)
                     /locus_tag="VFY80_05970"
     CDS             complement(2158..2697)
                     /locus_tag="VFY80_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6007488.1"
                     /translation="MPGFGEPTPRLMIVGLAPGMHGANRTGRPFTGDHAGIILYETLH
                     RFGFASQPTSEAIGDGLRLHRCRITNAVKCWPPRNKPLPDEIRRCNAYLSQELNALSR
                     GAAILALGVIAHQAVLDALGLPRAAHRFRHGAQYPLPVGYALFDSYHCSRYNTQTGRL
                     TADMFHQVFAAITTYLRAA"
BASE COUNT          493 a          860 c          843 g          525 t
ORIGIN      
        1 aatgatcgca tcgacacgtt cgagctgcac gagcgtgatg agtgctgccg cgaccatcag
       61 cttgtcggcg acgggatcga gaaactcgcc aaacgacgac gtctgattga gcttgcgggc
      121 aagccagccg tcgagccagt cggtcaccgc ggcggcggtg aagatgatcg ttgcgacgag
      181 gttctggttg ggcgcggaaa cccagctctt ctcgaagtaa aagatgccca cgaacagggg
      241 aatcagaacg atcctcagcc acgtgaggag attgggcagg ttgagcggca tggatgcggc
      301 ctaatgcagc tcctcgtaaa tcttttcggc cagcgtgcgg ctgatgcctt ccacctgcac
      361 catgtcgtcc acgctggctg ccatgaggcc gcgcagtcct ccaaaacgcg tgagcaattt
      421 ctgccgtcgt ttcgcgccca cgcccgcgat cgtttccagt ggagagctgc tgcgagcctt
      481 cgcccgccgc gcgcgatgcc cctgaatggc aaaacgatgc gcttcgtccc ggatctgctg
      541 gatcaggtgg agggcggggt gctcgggcgc aagggcaagg gccgcgtcgc gtccggcgat
      601 aaagagctgc tcgagtcccg gcttgcgccc ttcgcccttt gcgaccgcga ccagcatcac
      661 gtcgttcagg ccgagctcgg tgcacaccgc ctcagccact gcgagctgtc ccttgccgcc
      721 gtcgatcagt acgagatcgg gcatcctgcc ctcgcccgcc acgatcctgc ggtagcgtct
      781 tacgagcact tggcgcattg cctcgtaatc atcgcctggc attacccctt cgatgttgta
      841 gcggcgatat tcgcccttct gcagcccggc acgatcataa actacgcacg aggcaacggt
      901 cgcttccccc atggtgtggc tcacgtcgaa gcactcgatg cgctgagccg tctcggacag
      961 gccgagcgcg tgctgcaatg ccgacagccg cgcctcctgc gtcgcctgca gtcccacggt
     1021 ctgcgcggca ccgagcgctg cattcttcag cgccatgtcg agccatgccc gacgcccgct
     1081 cgaaggctgc gtaaccacct gcaccttgtg gtgagcccgg tccgacagca gtgtctcgaa
     1141 tgccgccgcg tcgactgccg cgccgacgac cacgttggca ggcgctccgt gatgcacgta
     1201 atgctgtgcc agaaaggcct cgatcacctc cgcatcggtg aaaccctccg cgttgcgcgg
     1261 aaagaaattc ttgtctccca agtgccgacc gccccgtatc attaccagat tcacgcacgt
     1321 tacgcccgct acgcgcgcgc acgcgatgac atcggcatcg ctcgccgatt cgccgctcac
     1381 gaactgcttt tcccgcacgc gccgcaacgc gccaatctgg tcgcgaaaaa gcgcagcgtc
     1441 ttcgtagcgg agggcctctg ctgcggcatt catgcggccg gcaagcctat cgattacctc
     1501 ctgttccttc cctttcagga acagctccgc gccgcgcacg tcctctgcgt agtccgcttc
     1561 ggaaacgagc cgcacgcacg gtgccgtgca ccgtttgatc tggtgcagca ggcagggccg
     1621 cgaccggttt gcaaacaccg tatcctcgca ggtgcgcagt cgaaagacct tttgcagaag
     1681 ctggatgctc tcgcgaacgg cgccggcgtt cgggaaggga ccgaagtaac ggttgcgctt
     1741 gtcgagcgcg ccgcgatgaa agcccaggcg cggatacgga tgaccggtga ccacgagata
     1801 tgggtaggac ttgtcgtccc taaacaagac gttatagcgt ggacttaacg ttttaattaa
     1861 attgctctcg agcagcagcg cctctccttc ggagcgggtg gccgtcactt caaccccggc
     1921 gacgtgcgag agcatggctt gcgttcgggg cgcgagcgcg gtgcgcttct ggaaatacga
     1981 agcgaccctt ttcttgagat tgagtgcctt gccgacgtac agcacctccc ccgcctcgtt
     2041 cagaaagcgg tacacgcccg gcagcgcggg cagattcgcg atgatttttg cggggtcagg
     2101 catgacagaa caatgtgggg ctgtacgcat tccgccggtt ccagcggcta cgcacgctca
     2161 tgcggcgcgc agatacgttg taattgcagc gaacacttgg tggaacatgt ccgcggtgag
     2221 gcggccggtc tgcgtattat agcggctgca gtgatagcta tcgaaaagcg cgtatccgac
     2281 tgggagcgga tattgcgcgc cgtgtcggaa gcgatgggct gcgcgcggga gtccaagcgc
     2341 atcgagtacg gcttggtgtg ctatgacgcc cagcgcgaga atcgctgccc cgcgcgacag
     2401 ggcgttcagc tcctgactca ggtatgcgtt gcagcgccgt atttcgtccg gcagcggctt
     2461 gttgcgcgga ggccagcact tgactgcatt tgtgattcgg cagcggtgca ggcgcagtcc
     2521 gtcccctatt gcttccgaag tcggctgact ggcgaatcca aaccggtgca acgtctcgta
     2581 aaggatgatg cccgcgtgat ctccggtgaa gggccgtccg gtacgattcg caccgtgcat
     2641 tccgggcgcg agcccaacga tcatgagtcg cggcgttggt tctccgaacc cgggcacagg
     2701 gcgagcgtag taatcgggat g
//
LOCUS       DASURG010000339         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_264491, whole genome shotgun sequence.
ACCESSION   DASURG010000339 DASURG010000000
VERSION     DASURG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264491"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFY80_05975"
     CDS             complement(<1..307)
                     /locus_tag="VFY80_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6007489.1"
                     /translation="MNDRKAPAGAAPPSAPRFARGPSLVTVAQLPEFDEIVDVRSEGE
                     YAEDHIPGAVSCPVLSDEERARVGTVYKQVSSFEAKKIGAALVSANIARHLKDRFASR
                     "
     gene            393..1451
                     /gene="selD"
                     /locus_tag="VFY80_05980"
     CDS             393..1451
                     /gene="selD"
                     /locus_tag="VFY80_05980"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:453..455,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX6007490.1"
                     /translation="MNKPSESANVRLTELAHGGGUGCKLTPALLSEVLTQSAASRVYS
                     DLLVGTETSDDAAVYRINETQAIVATTDFFMPIVDDPYDFGRIAATNAISDVYAMGAK
                     PFLALALLGMPVGKIAPEVIHRIVAGGRSVCDAAGIPIAGGHSIDSLEPIYGLVALGL
                     VDPRKVKRNSEAKAGDVLILGKPLGVGIMSAAIKKGQLSDRGYRELIETTTQLNTPGI
                     ALAEMEGVHALTDVTGFGLLGHLLGICRGSRVAAKLEWDRIPMLEEARQLAQQGLATG
                     ASQRNWASYGAEVRLPSAAPDWHRKVLTDPQTSGGLLVACEPHTAADVLKLFAAQGFH
                     RVGEIGRLEPGEPGVNVR"
     gene            complement(1469..2080)
                     /locus_tag="VFY80_05985"
     CDS             complement(1469..2080)
                     /locus_tag="VFY80_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX6007491.1"
                     /translation="MILRLLSMVLIALLAPGGALAATLLVFGDSLSAGYGLPREEAWV
                     SLLERKLREARPDYKVVNASVSGETTAGGRARIEQALETHRPALVILALGANDGLRGY
                     PIDALRANLEAIIETCRRHKAQVLLVGMQLPPNYGPAYTGKFRTAFAEIARKHALPSV
                     PFLLEGFAEKREYFQDDGLHPSAAAQPLIAETVWKALRPLLKP"
     gene            2079..>2673
                     /locus_tag="VFY80_05990"
     CDS             2079..>2673
                     /locus_tag="VFY80_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6007492.1"
                     /translation="MPGKDHAAGDANGAIVRVTALGKQVTTGEALLTILADVTFDVAQ
                     GEAVAIVGVSGSGKSTLLGLLAGLDTPSGGRVMLDGHDLYALDEDGRAALRARMVGFV
                     FQSFQLLPAMTALENVMLPLELAGALHARAAATAMLDRVGLGTRMHHYPRQLSGGEQQ
                     RVAIARAFVTRPALLLADEPTGNLDQTTGAQVIELMFE"
BASE COUNT          461 a          846 c          915 g          451 t
ORIGIN      
        1 gccggcttgc gaagcgatcc ttgagatggc gcgcgatatt cgcgctcacg agcgccgcgc
       61 cgattttctt cgcttcgaaa gaggaaacct gcttgtagac cgttccgacg cgtgcacgct
      121 cttcatcgct cagcaccgga cagcttactg cgccgggaat gtggtcttcg gcgtactcgc
      181 cttccgagcg cacatcgacg atctcgtcaa actcgggaag ctgtgctacg gttacgagcg
      241 atgggccgcg cgcaaacctc ggcgcagacg gcggtgcggc gccggcaggc gccttacgat
      301 cgttcatgcc ccggattcat ggcattctgt tcgcaatcca tcgcatcgcg ttcatgtgga
      361 tcatcgagag tgttgtccta gtttaagcga aaatgaacaa gccttctgag agtgcgaacg
      421 tgcgcctcac ggagctcgcc cacggcggcg gctgaggctg caagctcacg ccagcgttgc
      481 tgagcgaagt cctgacgcag tctgcggcga gccgggtcta ttcggacctt ctggtcggca
      541 cggagacgag cgacgatgcg gccgtctacc ggatcaacga gacgcaggca atcgtcgcga
      601 ccactgactt cttcatgccc atcgtggacg atccgtacga cttcggtcgc atagcggcga
      661 ccaacgcgat atcggatgtc tacgcgatgg gcgcgaagcc gtttctcgcg ctggcgctgc
      721 tcggcatgcc ggtcgggaaa atcgcaccgg aagtgatcca tcgcatcgtt gccgggggcc
      781 gctcggtatg cgacgccgcg ggcattccga ttgcgggcgg ccattcgatc gattcgttgg
      841 agccgatcta cgggctcgtc gcactgggac tcgtcgaccc gcgcaaggtg aagcgcaaca
      901 gcgaggccaa ggcgggcgat gtgctgattc tgggtaagcc gctcggtgtc ggcatcatga
      961 gtgcggcgat caagaaagga cagctgagcg accggggcta ccgggaactc atcgaaacga
     1021 cgacgcagct caacacgccg ggcatcgcgc tcgccgaaat ggaaggcgtg cacgcgctta
     1081 ccgatgtcac gggattcgga ttgctcgggc accttctcgg gatctgccgc ggttcacggg
     1141 ttgcggcgaa gctcgaatgg gaccgcatcc ccatgctgga agaggcccgc cagcttgcgc
     1201 agcagggcct tgcgacggga gcctcgcaac gcaactgggc gagctacggc gccgaagtga
     1261 ggttgccctc ggccgcaccg gactggcatc gcaaggtgct gacggacccg caaacgagcg
     1321 ggggactgct ggtcgcgtgc gagccgcaca cggctgcgga cgtcctgaag cttttcgcgg
     1381 cacagggttt ccaccgtgtg ggggagatcg ggcgtctcga gccgggcgag cccggcgtca
     1441 acgtccgcta ggcgggcgcc accgcccatt acggcttcag cagcgggcgc aacgctttcc
     1501 agacggtttc tgcgatgagc ggctgagccg cggcggacgg atgcaggccg tcgtcctgga
     1561 agtactcgcg cttctccgcg aatccctcca gcagaaatgg cacggacggc aatgcgtgct
     1621 tgcgtgcgat ttcggcaaac gccgtgcgga acttcccggt gtaggccggg ccgtagttgg
     1681 gcggcagctg catgccgacg agcagaacct gcgccttgtg gcggcgacag gtctcgatga
     1741 tcgcttcgag gttggcgcgc agggcgtcga tcgggtagcc gcgcaacccg tcgttcgccc
     1801 caagcgcgag gatgacgagc gcggggcggt gcgtctcgag tgcctgctca atgcgagccc
     1861 ggccgccggc cgtcgtttcg ccgctgacgc tcgcgttgac gaccttataa tcgggcctcg
     1921 cttcgcggag cttgcgctcg agcaggctga cccacgcctc ctcgcgcgga agcccgtacc
     1981 ccgccgacag gctgtcgccg aaaacgagca aggtcgctgc gagcgcgccg ccgggcgcga
     2041 gcagcgcgat gaggaccatg gaaagaagac gcaggatcat gccaggcaag gatcatgcag
     2101 cgggtgacgc gaacggggcc attgtgcggg tgaccgcgct cggcaagcaa gtgacgaccg
     2161 gagaggcgct gttgacgatc ctcgcggatg tgacgttcga cgtcgcgcag ggcgaggcgg
     2221 tcgccatcgt cggggtctca ggctcgggca aatccacgtt gctcggcctg ctggccggcc
     2281 tcgatacgcc gagcggaggt cgcgtgatgc tcgacggaca cgacctctac gcgctcgacg
     2341 aagacggccg cgcggcgctc agggcgcgca tggtcggctt cgtgttccag tcatttcagc
     2401 tgctgcccgc gatgacggcg ctcgaaaacg tcatgctgcc gctcgaactc gccggcgcgc
     2461 tccatgcgcg agccgctgcg acagcgatgc tggaccgcgt cggattgggg acgcgcatgc
     2521 atcattatcc gcggcagctc tcgggaggcg agcagcagcg cgtggccatt gcgcgcgcct
     2581 tcgtcacgcg gccggcgctg ctccttgccg acgagcccac cggcaacctc gaccagacaa
     2641 ccggcgcaca ggtgatcgag ctcatgttcg agc
//
LOCUS       DASURG010000340         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_264690, whole genome shotgun sequence.
ACCESSION   DASURG010000340 DASURG010000000
VERSION     DASURG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264690"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="queG"
                     /locus_tag="VFY80_05995"
     CDS             complement(<1..778)
                     /gene="queG"
                     /locus_tag="VFY80_05995"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX6007493.1"
                     /translation="MQGSGVPQSREIDFAALAHDIKRWGAALGFQEVGIAGCDMPEAE
                     TRLMEWLAQGRHGDMDYMARHGTKRARPAELVPGTLRIIAVRMNYAVSAQASRAVLDD
                     GRRAFVSRYALGRDYHKVLRRRLQRLADRISAAVGTFNYRAFVDSAPVMEVELARKAG
                     IGWRGKHTLSLSRDAGSNYFLGELYTDLPLPVDEASRDHCGSCSRCIDACPTGAIVAP
                     YQLDARRCISYLTIEHRGSIPEAFRRAMGNRIYGCDDCQLA"
     gene            773..1255
                     /gene="tsaE"
                     /locus_tag="VFY80_06000"
     CDS             773..1255
                     /gene="tsaE"
                     /locus_tag="VFY80_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6007494.1"
                     /translation="MHNADHKPADLNRFLASETDTLELGARLAAGLAPGMVVYLTGDL
                     GAGKTTLTRGVLRGLGYRGKVRSPTFTLVELYKFSSLYLYHFDFYRFGDPREWVDAGF
                     REYFTPQSVCLVEWPEKAAAGLPAADVTIALTLEGSGRRVKVYADTEAGRHCIERLGY
                     "
     gene            1231..>2127
                     /locus_tag="VFY80_06005"
     CDS             1231..>2127
                     /locus_tag="VFY80_06005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6007495.1"
                     /translation="MHRASRILTGGTPAAWTRGFRYSYLILALLWLDALLGASPALAQ
                     NGIPITAARVWPAADYTRITLESGTPIAHNLFGVKDPERLVLDLEGVDVTAALTQLAD
                     RIAPDDPYIKAVRIGRYKPGTVRLVLDLKTEVRPQAFILSPVGDYGHRLVLDLYPLVP
                     PDPLLAFLERAESRRAAEGDEASPSPPAAADAAEPGTVRAPRRKEPRATEPRSRQLII
                     VAVDAGHGGEDPGARGRRGTNEKDVTLAIARRLKARIDRERDMRAVLIRDGDFYIPLH
                     MRVQKARRVNADLFVSIHADAFI"
BASE COUNT          354 a          726 c          682 g          365 t
ORIGIN      
        1 aggcgagctg gcaatcatcg cacccgtaga tgcggtttcc catcgcccgg cggaaggcct
       61 ccgggatgct gcccctgtgc tctatcgtga gataggagat gcagcgccgc gcatcgagct
      121 ggtagggcgc gacgatggcg ccggtcgggc aggcgtcgat gcagcggcta cagctcccgc
      181 aatggtcgcg ggacgcctcg tccaccggca gcggcagatc ggtgtagagc tctcccagga
      241 aataattcga gccggcatcg cggctcaggg acaaggtgtg cttgccgcgc cagccgattc
      301 cggctttgcg cgcgagctcc acttccatca ccggcgcgct gtctacgaag gcccggtagt
      361 tgaacgtgcc gacggccgcg ctgatccggt cggcgagccg ctggagccgg cggcgcaata
      421 ccttgtgata gtcgcggccc agcgcgtaac gcgacacgaa ggcgcgcctt ccgtcgtcca
      481 agaccgcgcg gctcgcctgc gcgctgacgg cgtaattcat gcgtacggcg atgatccgca
      541 gggtgcccgg gacgagttcg gcgggacggg cccgtttcgt cccgtgcctt gccatataat
      601 ccatgtcgcc gtgacggcct tgggcaagcc attccatgag ccgcgtttcg gcctccggca
      661 tgtcgcagcc ggcaataccc acttcctgaa agccgagcgc agccccccag cgcttgatat
      721 cgtgcgcgag tgcggcgaag tcgatctctc gtgactgcgg aacgccagac ccttgcataa
      781 cgccgatcat aaaccagcgg atctgaaccg ctttcttgcc agcgaaacgg acactctcga
      841 gctcggcgcc cgcctcgccg ccgggctcgc gccgggtatg gtggtctatc tcaccggcga
      901 tctcggcgcc ggcaaaacca cgcttacccg gggtgttctg cgcggcctcg gctaccgcgg
      961 aaaagtgcgc agtccgacct tcacactggt tgaactttac aaattttcta gtttatactt
     1021 gtatcacttt gatttttatc gtttcggcga tccgcgtgaa tgggtcgacg ccggctttcg
     1081 cgagtacttc actccgcagt cggtgtgcct cgtggaatgg ccggaaaaag cggccgccgg
     1141 attgccggcc gccgacgtga cgatcgcgct cacgctcgaa ggcagcgggc ggcgcgtcaa
     1201 ggtttacgcg gatacggagg caggccggca ttgcatagag cgtctcggat actgacgggc
     1261 ggcacccccg cggcatggac gcgggggttt cgctattcat atctgatcct cgctctgctc
     1321 tggctcgatg ccttgctcgg cgcgagcccc gccctcgctc agaacggcat accgatcacc
     1381 gcggcgcgcg tatggccggc cgccgattac acccgcatca cgctcgaaag cggcaccccc
     1441 attgcccaca atcttttcgg cgtcaaggac cccgagcggc tggtgctcga tctggaaggc
     1501 gtcgacgtca cggcggccct gacccagctc gcggaccgta tcgctcccga cgatccttac
     1561 atcaaggcag tgcgcatcgg ccgctacaag cccggcacgg tgcggctggt gctcgacttg
     1621 aagaccgaag tccggccgca ggcattcatc ctgtcgccgg tgggcgatta cgggcaccgg
     1681 ctggtgctcg acctctaccc gttggtgccg ccggacccgc tgctcgcctt cctcgaaaga
     1741 gccgaatcgc ggcgcgccgc cgagggcgat gaggcgagcc cctctccacc cgcggcggcc
     1801 gatgcggccg aaccgggcac ggtgcgcgct ccccggcgca aggagccgcg ggcaaccgag
     1861 ccgcgctcga ggcagctcat catcgtcgcc gtggatgccg ggcacggagg cgaagacccg
     1921 ggcgcgcgcg gacggcgcgg caccaacgaa aaagacgtga cgctcgccat tgcgcgccgg
     1981 ctcaaggcac gcatcgaccg cgagcgcgac atgcgcgccg tgctgatacg cgacggcgac
     2041 ttctacattc cgctgcacat gcgcgtgcag aaagcgcgcc gcgtcaatgc cgacctgttc
     2101 gtctcgatcc acgccgatgc gttcatc
//
LOCUS       DASURG010000341         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_162033, whole genome shotgun sequence.
ACCESSION   DASURG010000341 DASURG010000000
VERSION     DASURG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162033"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFY80_06010"
     CDS             <1..1260
                     /locus_tag="VFY80_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX6007496.1"
                     /translation="DPFNVFLVALTAFVAFTTSLFSRPYMRIEAQDGRLSPRRLRLYH
                     SMFQMFTFTMLLALLTNNVGILWVAMEAATLTTVLLVSLYRTPASLEAAWKYFILCGV
                     GIAQALFGTILLYFAAEKVLGAGGTALLWTHLNEVKGALEPTVLGLAFVFLLVGYGTK
                     VGLAPLHNWLPDAHAEGPTPISAVLSGLLLNVALYAVVRAKVLVEGALGTHFAGNLMM
                     GFGLLSVVVAAFLLSRQKDIKRLFAYSSIEHMGLMTFAFGMGGAVATFAGLLHMTVHS
                     LTKSAIFFAVGHAAQKSGTQIMADIRGLIESNPTIGWGLLLGSLAILGMPPFGVFASE
                     FMLLTAAMHQEPWATPFLLAALGVAFAAIFGKVQPMVFGTASVRRLPHRPALIPVFVH
                     LAIVLLLGLYIPPYLAEWYRQAAQLIG"
     gene            1267..>2335
                     /locus_tag="VFY80_06015"
     CDS             1267..>2335
                     /locus_tag="VFY80_06015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6007497.1"
                     /translation="MKLEDFPFETRPLPGAMPVWVARVEREQWRSACEMVRDKGGRLV
                     ALWGADHTERGAGYIVYAALTVRSGLLIIALIAEGERYPDLSQVFPAANRMQRAVRDL
                     YGLEAEQAADTRKWLRHAAWPEEVFPLRKAFDARQTFPNGVDRYPFVEVGGEGVHEIP
                     VGPVHAGTIEPGHFRFSIVGEGILRLEERLGYKHKGIEKRFESMTLAEGAKLAGRISG
                     DSTVAYAWAYAMAVESVTASAPPPRALWLRALLLERERVGQHLWDLGFLGNDAGLAFG
                     LAQFSRLREDWLRTSHALFGHRYLMDVIVPGGVARDIRAESIVHMRSEAETIGQAVAS
                     LKTIYDEHAGLQDRFIGCGRVK"
BASE COUNT          350 a          743 c          789 g          453 t
ORIGIN      
        1 gaccctttca acgtctttct cgtcgcgctc acggcctttg tcgcgttcac gacatcgctt
       61 ttcagccggc cgtatatgcg catcgaagcg caggacgggc ggctttcgcc gcggcggctc
      121 cgcctctacc acagcatgtt ccagatgttc acgttcacca tgctgctcgc gctgctcacc
      181 aacaacgtgg gcatcctgtg ggtcgccatg gaggcggcga cgctcaccac ggtgctgctc
      241 gtgagcctct atcgcactcc ggcgagcctg gaagcggcgt ggaagtactt catcctgtgc
      301 ggggtgggca tcgcgcaggc gctcttcggc acgatcctgc tctatttcgc ggcggagaaa
      361 gttctcggcg cgggcggcac ggcgctcctc tggacgcatt tgaacgaggt caagggcgcg
      421 ctcgagccta cggttctcgg gctcgcattc gtgtttctgc tggtgggcta tggcaccaag
      481 gtcgggctcg cgccgctgca caactggctt cccgatgcgc acgccgaagg ccccacgccg
      541 atctccgcgg tgctttcggg actcctgctc aacgtcgcgc tctacgccgt cgtgcgcgcg
      601 aaggtgctgg tcgaaggggc gctcggcacg catttcgccg gcaatctcat gatgggattc
      661 ggactgctct cggtggtggt cgccgcattc ctgctctcgc gccagaagga catcaagcgg
      721 ctcttcgcct attcctcgat cgagcacatg gggctcatga ctttcgcctt cggcatgggc
      781 ggcgcggtgg cgacgttcgc ggggctgctc cacatgacgg tgcactcgct caccaagtcg
      841 gcgatcttct tcgcggtggg ccacgcggcg cagaaatccg gcacgcaaat catggcggac
      901 atccgcgggc tgatcgagag caatcccacc atcggctggg ggctgcttct cgggagcctc
      961 gcgatactcg gcatgccgcc cttcggcgtg ttcgcgagcg agttcatgct gctcaccgcc
     1021 gcgatgcacc aggaaccgtg ggccacgccc ttcctgctcg ccgcgctcgg cgtcgcgttt
     1081 gccgcgatat tcggaaaagt gcagccgatg gtattcggca cggcgagcgt gaggcgcctg
     1141 ccgcaccgcc cggcgttgat tccggtgttc gtgcatctcg ccatcgtgct gctcctggga
     1201 ctctacattc cgccgtatct cgccgagtgg tatcgccagg cggcgcagtt gatcggctga
     1261 gcggcgatga agctcgaaga ctttccattc gaaacgaggc cgctgcccgg cgcaatgccg
     1321 gtgtgggtcg cgcgcgtcga gcgcgagcag tggcgtagcg cgtgcgagat ggtgcgcgac
     1381 aaaggcggga ggctcgtagc attgtggggc gcggaccaca ccgagcgcgg tgccggttac
     1441 atcgtgtacg ccgcgctcac cgtgcgctcg ggattgctga tcatcgcgct catcgcggaa
     1501 ggggagcgtt atcctgacct gtcccaggtc tttcccgcgg cgaaccgcat gcagcgcgcg
     1561 gtgcgcgatc tctacgggct cgaggcggag caggcagcgg atacgcgcaa gtggttgcgg
     1621 catgccgcgt ggccggagga ggtctttccg ttgcgcaagg cgttcgatgc gcggcagacg
     1681 tttcccaatg gcgtcgatcg ctatccgttc gtggaagtgg gcggcgaagg ggtgcacgaa
     1741 attcccgtcg ggccggtgca cgccggcacg atcgagcccg ggcacttccg attttccatc
     1801 gtcggtgaag ggatcctgcg tctcgaagag cggctcggct acaagcacaa gggcatcgag
     1861 aagcgcttcg agtcgatgac gcttgccgag ggcgccaagc tcgccgggcg catttccggc
     1921 gattcgacag tcgcgtatgc gtgggcgtat gccatggcgg tggagagcgt gaccgctagc
     1981 gcgccgccgc cgcgcgccct gtggttgcgg gcgctgctgc tcgagcgcga gcgcgtcggc
     2041 cagcatctct gggatctcgg cttcctaggc aacgatgcgg gactcgcttt tgggctcgcg
     2101 cagttttccc gcttgcgcga agactggttg cgcacgagcc acgcgctctt cgggcatcgc
     2161 tacctcatgg atgtcatcgt tccaggcgga gtggcgcgtg acatccgggc cgagtcgatc
     2221 gtgcacatgc gcagcgaagc cgaaacgatc ggccaggcgg tcgcgtcatt gaaaacgatc
     2281 tacgacgagc acgccggtct gcaggaccgc ttcatcggct gcgggcgcgt gaaac
//
LOCUS       DASURG010000342         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_366399, whole genome shotgun sequence.
ACCESSION   DASURG010000342 DASURG010000000
VERSION     DASURG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366399"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFY80_06020"
     CDS             complement(<1..915)
                     /locus_tag="VFY80_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007498.1"
                     /translation="MRTPMRMKAWALLAIAAAFSVVSAPSAAQRWPTKPVRIIVPYPP
                     GGGTDIQARLLSAAFQKSMGHNFLIDNRVGASGLIGSQIAVDAPPDGDTLLFVSGNIS
                     VVPTLFAKHIKFDIYNDLAPVSWISSTPLVLTVHPSVPARTVPQLIALAKKHPGGMNA
                     TGNAAGTTAHLSGEMLNQIAGLKTTILNYRGGGPAVIALISGEADYGFTTAPSIMPHL
                     KSGKARGLAVTTPKRSSVLPDLPTMNTFYPGFEADNWYAMFYQKGTPRPIIDKMNAEI
                     KKALATPEIKAFYAKEALDPVASSPEELA"
     gene            complement(982..1488)
                     /locus_tag="VFY80_06025"
     CDS             complement(982..1488)
                     /locus_tag="VFY80_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6007499.1"
                     /translation="MTATVALTACAVEPAAVKQPALGTAVSAEELARWDISIPPSGAG
                     LPAGSGTARLGLPVYEQKCLACHGARGVGKPADALAGGTGSLASKAPLRTVGSYWPYA
                     TTLFDYVRRAMPLADPLSLSDDEVYAVSAYVLFLNGIIGEDFLLNAQTLPQVKMPNRN
                     GFLDMSRQ"
     gene            complement(1511..2803)
                     /gene="soxC"
                     /locus_tag="VFY80_06030"
     CDS             complement(1511..2803)
                     /gene="soxC"
                     /locus_tag="VFY80_06030"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX6007500.1"
                     /translation="MRPRSVAAAPSQELRDTAFAERRRNFFKQSAALVTGAASVGLGH
                     GANAQGLAAQPWERVYGSAFTGYGQPSRFEQPVVRHLLRPYGDLAPGTGGSLTPIESL
                     EGIITPSSLHMVRSHSGTPDIDPRKHQLLLHGLVARPLAFSMESLSRYAMITRTYFIE
                     CSGNSLRATLPKPVQMPAGAIHGMISCSEWTGIPLRVLLEEAGLQPHAKWLLAEGADS
                     AHMSRSIPLAKALDDAMIALYQNGERLRPEQGYPVRLLLPGWEGNTNVKWLRRLKVTE
                     GPTYARDETSKYTDLLPDGRAQQFTFEMGVKSVIAKPSSTMTLPGPGFYEISGLAWSG
                     AGRITRVEVSVDGGATWLDAALTGEKNAKCLVRFRLPWEWTGHPAVLQSRATDEKGRV
                     QPTRQEWVARYAPANIFHHNAIVSWAVSSAGSVTHVYA"
BASE COUNT          506 a          979 c          988 g          580 t
ORIGIN      
        1 ggccagctct tcggggctgc tcgcaaccgg atccagggcc tccttcgcat agaacgcctt
       61 gatctccggc gtcgcgagcg ctttcttgat ctccgcgttc atcttgtcga tgatcggtct
      121 cggcgtcccc ttctgataga acatcgcata ccagttgtcg gcctcgaagc ccggatagaa
      181 cgtgttcatg gtcggcagat ccgggagcac ggaggatcgc ttcggcgtcg tgacggcgag
      241 acctcttgcc ttgccgctct tcagatgcgg catgatggaa ggcgccgtgg tgaagccata
      301 gtcggcttcg ccggaaatga gtgcgatcac cgctggtccc ccgccacgat aattcagtat
      361 ggtcgtcttg agcccggcaa tctggttgag catttcgccg ctgagatggg cagtcgtccc
      421 tgcggcgtta ccggttgcat tcatgccgcc gggatgcttc ttcgccagcg cgatgagctg
      481 cggtaccgtc ctcgccggaa cgctcggatg caccgtcagc acgagcggcg tcgaggagat
      541 ccagctcact ggcgcgaggt cgttgtatat gtcgaacttg atgtgcttcg cgaaaagcgt
      601 cgggaccacc gagatgttgc cggacacgaa cagtagcgta tcgccgtccg gcggcgcgtc
      661 gaccgcgatc tggcttccga tcaggccgct cgcgccgacc cggttatcga tgaggaagtt
      721 gtgtcccatg ctcttctgga acgccgccga gagcagccgc gcctgaatat ccgtcccacc
      781 gcccggtggg tacggcacta tgatgcgcac gggctttgtc ggccatcgct gcgccgccga
      841 cggtgccgac acgacggaaa aagccgctgc aatggcaaga agcgcccagg ctttcattct
      901 catgggtgtt cgcattgcct cctccttgcg gtcagcgcta cttgtcgaat cgggcaacga
      961 gcaagcaggg ccagattgcg cctactggcg ggacatgtcc aggaacccgt tgcggttcgg
     1021 catcttcacc tgcggcagcg tctgcgcgtt caacaggaaa tcctctccga tgatgccgtt
     1081 gaggaacagc acataggcgc tcaccgcgta gacctcgtcg tcgctgagcg agagcggatc
     1141 ggcgaggggc atggcgcggc gcacatagtc gaacagcgtg gtcgcatacg gccagtagct
     1201 gcctacggtt cgcagcggag ccttggacgc gagcgagccc gtgccgccag cgagcgcgtc
     1261 ggcgggcttg cccacgcccc tcgcgccgtg acaagccaga cacttttgtt cgtacaccgg
     1321 caggcccagt cgcgcggttc cgctgcctgc gggcaggccg gcgccgctcg gcggtatgct
     1381 gatgtcccag cgcgcgagct cttccgcgct tactgccgtg cccagcgccg gttgtttcac
     1441 tgccgcaggc tctaccgcgc aggcggtcag ggctacggtt gcagtcacta cgcaggtgaa
     1501 tcgggctggc ctaggcgtag acatgggtca cgctcccagc gctgctcacc gcccagctga
     1561 cgatggcgtt gtgatggaag atattggctg gcgcatagcg tgccacccat tcctggcgcg
     1621 tcggctggac gcgccctttc tcgtccgtgg cgcggctttg caggacggcc ggatgccccg
     1681 tccattccca tggcaggcga aagcgcacca gacacttggc gttcttctcc ccggtgaggg
     1741 ccgcatcgag ccacgtcgcg ccgccatcga ccgatacttc gacgcgggtg atgcgcccgg
     1801 cgccgctcca cgcgagaccg gagatttcgt agaagcccgg acctggcagg gtcatcgtgg
     1861 acgacggttt ggcgatcacc gatttcacac ccatctcgaa ggtgaactgc tgagcgcggc
     1921 cgtcaggcag cagatcggtg tacttggagg tctcgtcccg ggcgtaggtg ggcccctccg
     1981 tgaccttcag gcggcgcagc cacttgacgt tcgtgttgcc ttcccagcct ggcaacagca
     2041 ggcgcaccgg gtagccctgc tccggccgca accgctctcc gttctgatag agcgcgatca
     2101 tggcgtcgtc gagcgccttc gcgagcggga tgctgcggct catgtgcgcc gaatcggcgc
     2161 cctccgccag gagccacttt gcatgtggct gcaatccagc ctcctccagc aggacccgca
     2221 gcggaatccc ggtccactcg ctgcacgaaa tcatgccgtg aatggctccg gcaggcattt
     2281 gcacgggctt gggcagggtg gcgcgcagac tgttgcccga gcactcgatg aagtacgtgc
     2341 gggtgatcat ggcgtagcgc gacagcgatt ccatggagaa cgccagcgga cgtgcgacca
     2401 gcccgtgcag cagcaactgg tgcttccttg gatcgatatc cggcgtgccg ctgtggctgc
     2461 gcaccatgtg cagcgaactg ggcgtgatga tgccttcgag cgattcgatc ggggtgagtg
     2521 acccgcccgt gccgggcgca agatcgccgt aagggcgaag caaatgccgc acgaccggct
     2581 gctcgaagcg cgagggctgg ccgtagcccg tgaacgcgct gccgtagacg cgctcccagg
     2641 gctgggcagc caatccttgc gcgttcgcgc cgtgaccgag gccgacggat gcggcgccag
     2701 tcactagcgc ggcgctctgc ttgaaaaagt ttcgacgccg ttccgcgaat gcggtatccc
     2761 gtaactcctg gctcggggcc gcagctacgc tccttggacg catatgagag ctctccctcg
     2821 aaggatccca gtgctgctat cgccagactg cctcgcgtcg ggattgcgcg ctagttcagt
     2881 aaatgaactt gtttcgccgc tgacggagga gtagcctgcc agcatgaagt ccggattcgg
     2941 ccagatatgc cccgtggcgg tggcatgcga ggtcttcgcg cagcgctgga cgccgataat
     3001 cctgcgcgag atgttcgccg ggtcacagca tttcaacgac atccatcgcg gcg
//
LOCUS       DASURG010000343         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1081158, whole genome shotgun sequence.
ACCESSION   DASURG010000343 DASURG010000000
VERSION     DASURG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081158"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFY80_06035"
     CDS             <1..1186
                     /locus_tag="VFY80_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX6007501.1"
                     /translation="GRICPAPCEEACVLRIIDDPVGIKSVEHAIIDHAWEEGWVQPQP
                     PQHRSGKRVAVVGSGPAGLACAQQLARAGHAVTVFEKNDRIGGLLRYGIPDFKLEKWI
                     IDRRLAQLEMEGVKFRTGVYVGRERPGKGVCDWAQEVLHPDLLLAEYDAVVLSGGAEL
                     PRDLPIPGRELDGVHFAMEFLPLQNRRVAGDRGVPDLRATGKHVIIIGGGDTGSDCVG
                     TSNRHGALSVTQLELLPMPPDVERNPVWPYWPTRLRTSSSHEEGVQRDWAVATKAFAG
                     ENGKVKALNAVRLEWRDGKPQEIPGSEFTLPADLVLLATGFVSPVQTILDEFGVAKDA
                     RGNARASTDGEGCYVTSAPTVFAAGDMRRGQSLVVWAIREGRQCARAVDEFLTGSSLL
                     PR"
     gene            1229..2284
                     /gene="hemE"
                     /locus_tag="VFY80_06040"
     CDS             1229..2284
                     /gene="hemE"
                     /locus_tag="VFY80_06040"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX6007502.1"
                     /translation="MLKNDTLVRALRRQPTPYTPIWIMRQAGRYLPEYNETRARAGSF
                     LRLCKSPNLCTEVTLQPLARFRLDAAILFSDILTIPDAMGLGLHFVEGEGPKFEYPLR
                     EEAEIRRLAAPDPDRELRYVMDAVSQIRRALKDEVPLIGFSGSPFTLACYMVEGGASA
                     DFRFLKTLLYSRPELLHRILEVNCRAVTAYLRAQIEAGAQVVMLFDTWGGMLSAAAYR
                     EFSLAYMKRVMDELAGNTSLDSVPRIVFTKGGGQWLEDIAGIGCDALGIDWTLDIGEA
                     RRRVGDRVAIQGNLDPSVLFASAATIRAETKRVLAAYGKAPGHVFNLGHGISQYTDPE
                     NVTALVEAVQEMSREFH"
BASE COUNT          434 a          717 c          759 g          436 t
ORIGIN      
        1 cggacgcatc tgtccggcac cgtgtgaaga ggcctgcgta ttgcggatca ttgacgaccc
       61 ggtcgggatc aaatcggtgg aacacgcgat tatcgaccac gcgtgggagg aaggctgggt
      121 gcaaccacaa ccgccgcagc atcgcagcgg caagcgcgtt gcagtggtgg gctccggtcc
      181 ggcggggctc gcgtgcgccc agcagctcgc ccgcgcaggg cacgccgtca ccgtgttcga
      241 gaagaacgac cgcatcggcg ggctgctgcg atacggcatt ccggatttca agctcgagaa
      301 gtggatcatc gacagacggc tcgcgcagct cgaaatggaa ggcgtcaagt tccgcaccgg
      361 cgtttacgtc ggcagggaac ggcctggaaa gggcgtgtgt gactgggcgc aggaagtcct
      421 ccatcctgat ctcctgcttg ctgagtacga tgcggtagtg ctgagcggcg gggcggagct
      481 tccacgcgat ttgcccatcc ccggacgtga gctcgatggc gtacatttcg cgatggagtt
      541 cctgcccctt caaaacaggc gtgtggcggg cgaccgtggc gtaccggatc ttcgtgcgac
      601 ggggaagcac gtgatcatca tcggcggcgg ggatacgggt tcggactgcg ttggcacgtc
      661 gaatcgccat ggcgcgctct ccgttacgca gctcgagctt cttcccatgc cgccagacgt
      721 cgaacgcaat ccggtctggc cctactggcc gacacgcctg agaacctcct catcacatga
      781 agagggcgtc cagcgcgatt gggccgtcgc caccaaggca ttcgccggcg aaaacgggaa
      841 agtgaaagcg ctgaatgcgg tgcgcctcga gtggcgcgac gggaagcctc aggagatccc
      901 cggttccgaa tttacgcttc ctgcggacct cgttctgctc gctacagggt tcgtctctcc
      961 cgtgcagacc atactggatg agttcggtgt cgctaaggat gcgcgaggca atgctcgcgc
     1021 gtcgacggac ggcgaaggct gctatgtcac ctcggccccg acggtattcg ccgcaggcga
     1081 catgcgacgc ggccagtccc tcgtagtatg ggcgatacgc gaggggcggc agtgcgcccg
     1141 cgcggtagac gagtttctga ccggcagctc gctgctgccg cgctgaggtt cgccgacgcg
     1201 ctctcctgtt cggcgccaaa gtcgtcccat gctcaagaac gatacgctcg tacgcgccct
     1261 gcggcgccag ccgacgcctt acacgccgat ttggatcatg cgccaggccg gacgctatct
     1321 gccggaatac aacgaaacgc gcgcccgagc cggcagcttc ctcaggctct gcaaaagccc
     1381 caatctttgc accgaggtca cgctgcagcc gctcgcacgt ttcaggctgg acgcggccat
     1441 cctgttttcg gatattctga ccataccgga cgcaatgggc ttgggcctcc atttcgtcga
     1501 gggggaaggg cccaagttcg aatatcctct gcgcgaagag gccgagatcc ggagacttgc
     1561 cgctccagat cctgaccgtg aactgcgcta cgtcatggac gccgtctccc agatccggcg
     1621 tgcattgaaa gacgaggtgc cgctcattgg attttccggc agccctttca cgctcgcctg
     1681 ttacatggtg gaaggcgggg cgagcgccga tttccgattc ctgaagacgc tgctctactc
     1741 tcggcccgag ctgttgcacc gcattctcga agtcaactgt cgcgcagtga ccgcctatct
     1801 acgagcgcag atcgaagccg gcgcacaggt cgtaatgctt ttcgacactt ggggcggcat
     1861 gttgagtgcg gcggcttacc gcgaattctc gctcgcttac atgaagcgcg tgatggacga
     1921 gttggccggc aacacgtcac tggactccgt gcccaggatc gtttttacca agggcggcgg
     1981 ccagtggctc gaggacattg ccggaatcgg atgcgatgcg ctcggcatcg actggacgct
     2041 ggacatcggc gaggcgcgac gccgggtagg cgatcgcgtc gcgatccagg gcaatctgga
     2101 tccctccgtg ctctttgcga gtgccgccac gatacgagca gaaacaaagc gggtactggc
     2161 tgcatacggg aaagcccccg gtcatgtttt caacctcggg cacggcattt cgcaatacac
     2221 cgatccggaa aatgtaacgg cgctggtgga agcggtgcaa gagatgagcc gcgagtttca
     2281 ttgaaagcct cggcgcgaca cttggcgtcg ggttgcgggc cagacgccgg aatcgggttc
     2341 cgacac
//
LOCUS       DASURG010000344         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_673339, whole genome shotgun sequence.
ACCESSION   DASURG010000344 DASURG010000000
VERSION     DASURG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673339"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2749)
                     /locus_tag="VFY80_06045"
     CDS             complement(<1..2749)
                     /locus_tag="VFY80_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2201..2203),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6007503.1"
                     /translation="MRLTGAGSATALAGLAALGANLAPARARAQSLRIKDSRTVPSIC
                     PFCSVGCATLIHVQNGEIINIEGDPRSPHNEGTLCPKGAAIYQLHKNPNRPQKVLHRK
                     PGASEWEEVDLGWAMDRVAELTRKTRDETFVERLPNGKLVNSTTAIFHLGGATLDNEF
                     NHMAQKFLRAYGIVAIENQARIUHSSSVPGLGARLGRGAATTYPRNLEQTDCMVIMGS
                     NMAECHPVAFRWPMKAKVNGAKIIHIDPRFTRTSAVADLHVPIRAGSDIAFLGGLINY
                     VLNSARWNRDPFFRDYVANYTNAATLVNEQFRDTEDLNGVFSGLSTYTEPAFWPYNAF
                     VGNYRTDTWQYDRGAGQPGPTTGTQGQMAPGRPAPTAQAGETPEGRRPGSPGTAGDPS
                     GPQRFGPGYDDLVRSLRQPAPQRDPTLQNPRCVLQILRRHFARYTPEVVEQICGTPKE
                     KFLKVAELLLENSGRDRTSAFAYAVAWTQHTTGVQIISCAALLQLLLGNIGRPGGGIM
                     ALRGHAAIQGSTDVPTLYHSIHGYMSHPSALKQHDSLEEYLRTETSPGGYWANTPKFM
                     VSYLKSFYGDAATPANNFGYDWHPKIVGDHSHMAMFSAMADGKVKGMFCVGQNPATSI
                     AARVERAGLRKLEWLVVKDNWLTETATYWHSAPEIKDGTISPADIPTEVFFFPSTQIA
                     EYDGSFTNTQRMLQWHFMAAKAPGDCRTDTWFYHQLAKRLKNAHANSRHARDQGWLNT
                     LWDFDPDPGTEALYPGEPDAAKILREINGYMTGNPNQHLPGFAALKDDGSTTCASWIY
                     CGCFPSPDNNLTAARNPDPPNQPGAQLGWGFAWPANRRVMYNRASADLDGNPWSERKR
                     WVWWDGQKWTGYDVPDFAATKPPSAPANPNAVGLDGLAGTDAFIMKGDGKGWLFVPAG
                     LV"
BASE COUNT          466 a          868 c          896 g          525 t
ORIGIN      
        1 ccaccaggcc cgcgggcacg aagagccagc ccttgccatc gcctttcatg atgaacgcat
       61 cggtaccggc cagtccgtcc agaccgacag cgttcggatt cgcgggggcg ctgggcggct
      121 tcgtcgccgc aaagtccggc acgtcatagc ccgtccactt ctgcccatcc caccagaccc
      181 agcgcttgcg ttcactccac ggattgccat ccagatccgc cgatgcgcgg ttgtacatca
      241 cgcgccgatt ggcgggccac gcgaatcccc aaccgagctg cgcgcccggc tgattgggcg
      301 gatcgggatt gcgcgccgca gtcagattgt tgtcgggaga agggaagcag ccgcagtaaa
      361 tccacgaggc acacgtggtc gagccatcat ccttgagcgc ggcaaagccg ggcagatgct
      421 ggttcggatt gccggtcatg tagccattga tctcgcgcag gatcttggcg gcgtccggct
      481 ctcccggata aagcgcttcc gtgcccggat ccggatcgaa atcccacagc gtgttgagcc
      541 acccctggtc gcgcgcatga cggctgttgg cgtgcgcgtt cttcaaccgc ttcgccagct
      601 gatgatagaa ccaggtgtcg gtgcggcaat cacccggcgc cttggcggcc atgaagtgcc
      661 actgcaacat gcgctgcgtg ttggtgaagg agccatcgta ctcggcgatc tgcgtcgacg
      721 ggaagaagaa tacctccgtc ggaatgtccg ccggactgat ggtgccatcc ttgatttcgg
      781 gcgcgctgtg ccagtaagtc gccgtctcgg tcagccagtt gtccttgacg accagccatt
      841 cgagcttgcg caagccggcg cgctcgaccc gtgccgcaat cgacgtggcc gggttttgcc
      901 cgacgcagaa catgcctttg actttgccat ccgccatcgc ggagaacatg gccatgtgcg
      961 aatggtcgcc cacgatcttg ggatgccagt cgtaaccgaa gttgttggcc ggcgtggccg
     1021 cgtcaccata aaacgacttc aggtacgaga ccatgaactt gggtgtattg gcccagtagc
     1081 cgcccggcga tgtctcggtg cgcagatact cttccagcga gtcgtgctgc ttgagcgcgg
     1141 agggatgcga catgtagccg tggatgctgt ggtacagcgt cggcacatcg gtcgaaccct
     1201 ggatggccgc gtgcccgcgc agtgccatga tgccgccgcc cggacggccg atattgccga
     1261 gcagcaattg cagcagtgcc gcgcacgaga tgatctgcac gccggtcgtg tgctgcgtcc
     1321 acgcgaccgc gtaggcgaac gcgctcgtgc ggtcgcgccc ggagttctcc agcagcagct
     1381 ccgcgacctt gaggaacttt tccttcgggg taccgcaaat ctgctcgacg acttccggcg
     1441 tgtagcgcgc gaaatggcgt cgcaggatct gcagcacgca gcgtggattc tggagtgtcg
     1501 ggtcgcgctg cggcgccggc tggcgcagcg aacgcaccag atcgtcgtag ccggggccga
     1561 agcgctgcgg gccgctcgga tcgcccgctg tgccgggcga acccggacgc cggccttccg
     1621 gcgtttcacc cgcttgcgcg gtcggtgccg gacgccccgg cgccatttgc ccttgcgtgc
     1681 cggtcgttgg acctggctgc ccggcgccgc ggtcgtactg ccacgtgtcc gtgcgatagt
     1741 tgccgacgaa tgcgttgtac ggccagaacg ccggctcggt atacgtcgac aagcccgaga
     1801 acacgccgtt cagatcttcc gtatcgcgga attgctcgtt gaccagcgtg gccgcgttcg
     1861 tgtagttggc tacataatcg cggaagaagg gatcgcgatt ccagcgcgcg ctgttgagga
     1921 cgtagttgat gagcccaccc aggaacgcaa tgtcgctgcc ggcccggatc ggcacgtgca
     1981 aatcggccac cgcgctggtg cgggtgaagc gcggatcgat gtgaatgatc ttggcgccat
     2041 tcaccttggc tttcatcggc caacggaatg caaccggatg acattccgcc atgttggagc
     2101 ccatgatgac catgcaatcg gtctgctcca ggttgcgcgg atacgtcgtc gcggcgcctc
     2161 ttcccaatcg tgcccccaga ccggggacgc tagagctgtg tcatatccgg gcctggtttt
     2221 ctatggcgac gatgccatag gcgcgcagga acttctgcgc catgtgattg aactcgttgt
     2281 cgagcgtcgc accgcccaga tggaagatgg ccgtcgtcga attgacgagc ttgccgttgg
     2341 gcagccgctc gacaaaggtc tcatcgcgtg tcttgcgcgt gagctcggcc acccgatcca
     2401 tggcccagcc caggtcgacc tcctcccatt ccgaggcgcc cggcttgcgg tgcagtacct
     2461 tctgcgggcg attgggattc ttgtgcagct gatagatcgc ggcgcccttc ggacagagcg
     2521 tgccttcatt gtgcggtgag cgcggatcac cctcgatgtt gatgatctcg ccattctgca
     2581 catgaatcag cgtagcgcag ccgacggagc agaagggaca aatgctggga acggtccggc
     2641 tgtccttgat gcgcagactc tgcgcacggg cgcgagcggg agcgagattg gcgccgagcg
     2701 cggcgagtcc ggccagggcg gtggcgctgc ctgcaccggt gagccgcaga aacgc
//
LOCUS       DASURG010000345         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_366647, whole genome shotgun sequence.
ACCESSION   DASURG010000345 DASURG010000000
VERSION     DASURG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366647"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFY80_06050"
     CDS             <1..982
                     /locus_tag="VFY80_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007504.1"
                     /translation="LPAFVRTVLGALLCVAAIDAVWAAQPTGSYPNRPIRVVVPFTPG
                     GQPDIVARLIAPRLGETLGQQVVVDNRPGAGGMIGARIVAGANPDGHTLLSVSAAHVV
                     TPAVRANLGYDPVKDFAGITMTINSGYFLVVPPALPVKNVKDLVALAKAKPGQLNFSS
                     AGRGSSTHFAAEVFKQTAAIDVVHVPHRGIPEALTEIMTGRAQFFMAPFASAGALVKE
                     GRMRALGVTTAQRSRAYPDIPTLSEAGVPGFRYDSWGAMFAPAKTPRAIIDRLNREVV
                     AILKHPETGQRLTALGVEATPTTPQQLDKFIAEQVPASLELARRAGITAE"
     gene            1256..1465
                     /locus_tag="VFY80_06055"
     CDS             1256..1465
                     /locus_tag="VFY80_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007505.1"
                     /translation="MKPKKTWHRRAKSMLVVAPASARTWVVAHSRRLIPGALFHTREA
                     AVRYAVVLAQAAGLRPTQVKVLAAA"
     gene            1518..2153
                     /locus_tag="VFY80_06060"
     CDS             1518..2153
                     /locus_tag="VFY80_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX6007506.1"
                     /translation="MPGMKPHRCAKSLLVVAALAATAAPLGAQQLYKWVDERGVTNYS
                     NQPPPDPKTARNVRQVEDRLSVYTPDAGLTQAIEDSNRNFEDRQRARQKIEALETQLE
                     AERRAREQAAAAARDNQAAYDRCIDDGRIDCGDVYGTYYPYAPVVGRHGRRLPQPHLT
                     PGATAGNVTAGNSFIPGNSASAPPPPRKESPRRGRDNPRHSSPSERPLERR"
     gene            complement(2150..>2872)
                     /locus_tag="VFY80_06065"
     CDS             complement(2150..>2872)
                     /locus_tag="VFY80_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX6007507.1"
                     /translation="RQLSTTLLQIENEFYGTIRPKRRIRPGERPLHALSERGVEYVEV
                     RCMDLDPFSPIGIKADTMRFLDVFLLHCLLSESPPDTPQEIGAKARNQHRVALRGREP
                     ELKLERRGGEVPLTDWADEVLGACQPLAAALDRAHDTTAYGNVVATARRAIAEPSSTP
                     SARVLREMKSAHEDSYLKFALERSLQHRGYFKALPWDAVLEERYTRLAEASRDEQNQI
                     EADDAVPFETYRRQYLALDLLN"
BASE COUNT          508 a          952 c          910 g          502 t
ORIGIN      
        1 actacctgcg ttcgtgcgaa cggtcttggg tgcgctcctg tgcgtggcgg cgatcgatgc
       61 cgtctgggca gcgcagccca ccggaagcta tccgaaccgg ccgatccgcg tggtggtgcc
      121 ttttaccccc ggcggccagc ccgacatcgt tgcgcgcctc attgcgccga gactcgggga
      181 aacgctcggg cagcaggtgg tggtggacaa tcgcccgggc gcaggcggca tgatcggcgc
      241 gcgcatcgtg gccggtgcga acccggacgg acatacgctg ctctccgtat cggccgcgca
      301 tgtcgtgaca ccggccgtgc gggcgaacct cggatacgat ccggtgaagg acttcgccgg
      361 catcacgatg acgatcaact ccggatactt tctcgtcgtt ccgcccgcgc ttcccgtcaa
      421 gaacgtgaag gatctcgtcg cgctggcgaa agctaagccc gggcagctca acttctcctc
      481 cgcgggacgg ggcagcagca cgcactttgc cgcagaggtc ttcaagcaga cggcggccat
      541 cgacgtcgtc catgtgcctc accgcggcat tccggaagcg ttgaccgaga tcatgaccgg
      601 gcgcgcgcag ttcttcatgg cgcctttcgc gagcgccggc gcactggtca aggaaggccg
      661 gatgcgggcg ctcggtgtca cgaccgcgca gcgcagccgt gcgtatcccg acattccgac
      721 gctcagcgag gctggagtgc ccggattccg ctacgattcg tggggcgcga tgtttgcgcc
      781 ggcaaagacg ccgcgcgcga tcatcgaccg cctcaaccgc gaagtcgtcg ccatcctgaa
      841 gcaccccgaa accgggcagc gcctgaccgc gctcggtgtc gaggcaaccc ccaccacgcc
      901 gcagcagctc gacaagttca tcgccgagca ggtgcccgca tcgctcgagc ttgcacgcag
      961 ggcaggcatc accgccgaat gacgcaggca taaccgcatc actccggcac gatctttcac
     1021 tgttctgtcg cccgcctacg acactttgcg cgccacatcg gcgccgtgca gttgtctcgt
     1081 gctataaggg ctatctactt tacaccttgc ctctcaagga tttagtttcg tgccgacgct
     1141 gcttcgtcgg cccccgctag cgagctggtt cggttcttgc gtgatatcgg gatcgggaag
     1201 catcgcactt cccttctcga gtgtcgaaac gcttcaatta aggaggaccg caccgatgaa
     1261 accgaagaaa acatggcatc gcagggcaaa atccatgctg gttgtcgccc cggccagcgc
     1321 gagaacctgg gttgtcgcac actcgcgacg gctgataccc ggggcgctct tccacacgcg
     1381 cgaagcagcc gtacgctatg ccgtcgttct ggcgcaggcg gcggggctgc gtcccacgca
     1441 agtgaaggtg ctggctgccg cctagcgacc tcctgcgtaa catttcgtta cccctgccgg
     1501 cggaggattt tgctagcatg ccgggtatga aaccccaccg ctgcgcaaag tccttgctcg
     1561 ttgtcgccgc gctcgcagcc acggcagcgc cgctcggcgc gcaacagctc tacaagtggg
     1621 tggacgagcg cggcgtgacg aactactcga accagccgcc tcccgatccg aaaactgcaa
     1681 ggaacgtgcg gcaagtagaa gaccgcctgt cggtctatac accggatgca ggactgacac
     1741 aggctatcga agattcgaac cgaaacttcg aggatagaca gcgcgcgcgg cagaaaatcg
     1801 aggcgctcga gactcagctc gaagccgagc ggcgtgcgcg cgagcaggca gccgcggccg
     1861 cgcgcgataa ccaggccgcc tatgatcgat gcatcgatga tggccggatc gattgcggtg
     1921 acgtgtacgg cacctattat ccgtatgcac ccgtggtggg ccggcacggt cggcggctgc
     1981 cgcagccgca cctcacgccg ggcgcgacgg ccggcaatgt caccgccgga aacagtttca
     2041 ttcccggcaa ttccgcctcg gcaccgcctc ccccgcggaa ggaatcgccg cggcgtggcc
     2101 gcgacaatcc gcggcacagc tcgccttccg agcgcccgct cgagcgccgt tagttcagta
     2161 gatcgagagc gagatactga cgccggtagg tctcgaacgg cactgcgtcg tcggcctcga
     2221 tctggttttg ctcgtctcgt gacgcctccg cgagccgcgt gtaccgctcc tccaaaaccg
     2281 catcccacgg cagcgccttg aaatagccgc ggtgctgcaa cgaacgctcg agcgcgaatt
     2341 tgaggtacga atcttcgtgc gcgctcttca tttcgcgcag cacgcgtgca gacggcgtgg
     2401 atgaaggctc cgcgatggcg cggcgtgcgg ttgcgacgac attgccatat gcggtcgtat
     2461 cgtgggcgcg gtccagcgcc gccgccaacg gttggcaggc gcccagcacc tcatcggccc
     2521 aatcggtgag cggcacttcg cctccacggc gctcgagttt cagttcgggt tcccttcccc
     2581 gcaacgcgac gcggtgctga ttgcgcgctt tggcgccgat ctcttgcgga gtatcgggcg
     2641 ggctttcgct caagaggcag tgcaggagaa agacgtcgag aaagcgcatg gtgtccgcct
     2701 tgatgccgat cggcgaaaac ggatcgaggt ccatgcagcg cacctccacg tattcgacgc
     2761 cccgctcgga aagggcgtgc agcggccgct cgcccggccg gatacgtcgt ttgggccgga
     2821 tcgtgccgta gaactcgttc tcgatctgca gcagcgtggt ggagagctgg cg
//
LOCUS       DASURG010000346         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_979597, whole genome shotgun sequence.
ACCESSION   DASURG010000346 DASURG010000000
VERSION     DASURG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979597"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /gene="mfd"
                     /locus_tag="VFY80_06070"
     CDS             <1..2063
                     /gene="mfd"
                     /locus_tag="VFY80_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6007508.1"
                     /translation="RRDRDTRRATSEGMLRDLSEVKIGDPVVHEQHGIGRYLGLSTMD
                     LGEGETEFLTLEYAGGDKLYVPVSGLHLISRYSGAAPEAAPLHQLGSGQWEKAKKKAA
                     AQVRDTAAELLNLYAQRASRKGHAFGIRQHDYEAFAEGFPFEETPDQAAAIHATLEDL
                     KSGKPMDRLVCGDVGFGKTEVALRAAFIAIADGKQVAVLVPTTLLAEQHFNTFSDRFA
                     DWPVRIAELSRFRSAKESAEALKGLAEGRIDIAIGTHKLLGREVKFRNLGLVIIDEEH
                     RFGVRQKERLKALRAEVDVLTLTATPIPRTLAMSLEGLRDFSVIATAPQRRLAIKTFV
                     SRHSRALIREAVMRELKRGGQIYFLHNDIDTIHHVEETLTKLVPEARIRVAHGQMRER
                     ELEHVMRDFYQQRFSLLLCTTIIETGIDVPSANTIIINRADKFGLAQLHQLRGRVGRS
                     HHQAYAYLLLPDEGNITAQARKRLEAIQMMEELGSGFFLAMHDLEIRGAGEVLGESQS
                     GEMQEVGFNLYCAMLDAAVRSLKQGKEPDLTAPLGVTTEINLHAPALLPSDYALDVHE
                     RLVLYKRLANCEDEEALNVLREELVDRFGPLPEPARTLIDSHRLCILGKPLGIARVDA
                     TDAAVQLQFVPQPPIEPIKVLKLVQSRRNYRLAGQDRLRIEAAMPDVKTRVAAIHDVF
                     SALH"
     gene            2159..2410
                     /locus_tag="VFY80_06075"
     CDS             2159..2410
                     /locus_tag="VFY80_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007509.1"
                     /translation="MKSTAAVVALIAGLSAAPLAAQTKDAKPQAAAPSAEKGVVLKQG
                     AQPAPAKRARTLASVDARHCLQLTTNMAIHRCAEKYRPR"
     gene            2534..>3237
                     /gene="serB"
                     /locus_tag="VFY80_06080"
     CDS             2534..>3237
                     /gene="serB"
                     /locus_tag="VFY80_06080"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX6007510.1"
                     /translation="MHLVIQGADVATPDLKELAKRSGASRIERLTPEAFRLVDAHPAD
                     GIESFCESRQLDCALVPESRRLADFRLLVMDMDSTLITIETIDELADLVGLKQEVAAI
                     TARAMRGEIEYDESLRHRVALLQGLDASALDRVYDERLKLTPGAEKLLAAVKRAGMKT
                     LLVSGGFTRITARLQPRLGLDYTYSNTLEVRDGKLTGSVVGRIVNADGKRDELVRVRE
                     MLGIDRSQVIAIGDGA"
BASE COUNT          615 a         1082 c         1033 g          507 t
ORIGIN      
        1 cgcgccgcga ccgcgacacc cgccgcgcca cgagcgaagg catgctgcgc gatctttcgg
       61 aggtgaagat cggtgacccg gtagtgcacg agcagcatgg catcggccgc tatctcggtc
      121 tcagcacgat ggatctcggc gagggcgaaa ccgagttctt gacgctcgaa tacgcgggcg
      181 gcgacaagct gtacgtgccg gtgtcgggcc tgcatctcat cagccgctac agcggcgcgg
      241 cgcccgaagc ggcaccgctg catcagctcg gcagcggcca gtgggagaaa gcgaagaaga
      301 aagccgccgc gcaggtgcgc gatacggcgg cagaactcct caacctctac gcccagcgcg
      361 cctcgcgcaa aggccacgcg ttcggcatcc ggcagcatga ctacgaagcg ttcgccgagg
      421 gcttcccgtt cgaagagacg cccgatcagg cggccgccat ccatgccact ctggaagatc
      481 tgaagagcgg caagccgatg gatcgcctgg tctgcggcga tgtcggcttc gggaaaaccg
      541 aagtcgccct gcgcgccgca tttatcgcga tcgccgatgg caagcaagtc gccgtgctcg
      601 tgccgacgac cctcctcgcc gagcagcatt tcaatacctt cagcgaccgc ttcgcggact
      661 ggccggtcag gatcgccgag ctttcgcgct tccgcagcgc gaaggaatcc gcggaagcgc
      721 tgaaggggct cgccgaaggg cgaatcgaca tcgcgatcgg cacgcacaag ctcctcgggc
      781 gcgaggtgaa attcaggaat ctgggactcg tcatcatcga tgaagagcat cgcttcgggg
      841 tgcgccagaa ggagcggttg aaggcgctgc gcgctgaggt ggatgtcctc acgctcaccg
      901 cgacgccgat cccgcgcacg ctcgcgatgt cgctcgaagg cctgcgcgat ttctcggtca
      961 tcgcgaccgc gccgcaacgg cggctcgcga tcaagacctt cgtttcgcgc cacagccgcg
     1021 cgctgatccg ggaggcggtc atgcgggagc tcaagcgcgg cgggcagatc tacttcctgc
     1081 acaacgacat cgacaccatc catcacgtcg aggagacgtt gacgaagctc gtgcccgaag
     1141 cgcgcatccg ggtcgcccac ggccagatgc gcgagcgcga gctcgagcac gtgatgcgcg
     1201 acttctacca acagcgcttc agccttctgc tctgcacaac catcatcgaa acgggcatcg
     1261 acgtgccgag cgccaatacg attatcatca accgcgcgga caagttcggc ctcgcgcagc
     1321 ttcatcagct gcgggggcgc gtcgggcgct cgcaccatca ggcctacgcc tacctgctgc
     1381 tgcccgatga aggcaacatc acggcgcagg cgcgcaagcg gctggaagcg atccagatga
     1441 tggaggagct gggttcgggg tttttccttg ccatgcacga cctcgagatc cgcggtgccg
     1501 gcgaagtgct cggagagtcg cagagcgggg aaatgcagga agtcggcttc aacctctact
     1561 gcgcaatgct cgatgcggcc gtgcgctccc tcaagcaggg caaggagccc gatctgacag
     1621 cgccactcgg cgttaccacc gaaatcaacc tgcacgcgcc ggcactgctg ccgtcggact
     1681 acgcgctcga cgtccacgag cggctcgtgc tctacaagcg gctcgccaac tgcgaggacg
     1741 aggaggcgct caacgtgctg cgcgaggagc tggtggaccg ctttggaccg ctgcccgagc
     1801 ccgcgcgcac gctgatcgac agccaccggc tgtgcattct cggcaagccg ctcggcattg
     1861 cgcgcgtcga cgcgaccgat gccgccgtgc agcttcagtt cgtgccgcag ccgccaatcg
     1921 agccgatcaa ggtcctgaag ctcgtccaga gccgccgcaa ctaccgcctc gccgggcagg
     1981 acaggcttcg catcgaggcg gcgatgccgg acgtaaagac ccgggttgcc gccattcatg
     2041 acgtatttag cgccctccat tgacggttcg tctcggcaac gtgtgtgacg gaggacttag
     2101 tggtggcgcc ccttagatgg taagcttgcc ccggtgcccg aaaacaaaag gaggatccat
     2161 gaagagcaca gccgccgttg tcgcactgat cgcagggctc tccgccgcac cactggcagc
     2221 gcaaacgaag gacgcgaagc cgcaggccgc cgccccgagc gcggagaagg gcgtagtact
     2281 caagcagggt gcccagcctg cgccggccaa gcgcgcgcgc acactcgcca gtgtggatgc
     2341 acggcactgc ctgcaactca caacgaatat ggccattcac cggtgcgccg agaagtatcg
     2401 gccgcggtag aaatcacccc gtcggtcccg atcgtccgct cttggctcgt catcccggcg
     2461 aaagccggga tccagcacct ccccgcttct cacctcgtca tttcgggtga aagcgggatc
     2521 caggcatagc ctcatgcatc tcgtcatcca gggcgccgat gtcgcgactc ccgacctcaa
     2581 ggaactcgca aaacggtcgg gcgcctcgcg catcgagcgc ctgacgcccg aggcattccg
     2641 cctcgtagac gcgcatcccg cggacggcat cgaaagcttc tgtgaaagcc ggcagctcga
     2701 ctgcgcgctc gttccggagt cgcggcggct cgccgacttc cggctgctcg tcatggacat
     2761 ggactcgacg ctcatcacga tcgagaccat cgacgagctc gccgatctcg tcggactgaa
     2821 gcaggaagtc gcggcgatta ccgcacgtgc gatgcgcggc gaaatcgaat atgacgaaag
     2881 cctgcgccac agggtcgcgc tgcttcaggg actggacgcg tccgcgctcg atcgcgtgta
     2941 cgacgagcgc ctcaagttga cgccgggcgc cgagaaactg cttgccgcgg tgaagcgggc
     3001 cggcatgaag acgctcctgg tctccggcgg attcacgcgc atcaccgcgc gccttcagcc
     3061 gcgtctgggg ctcgattaca cctattccaa cacgctcgag gtgcgcgacg gcaagctcac
     3121 gggcagcgtc gtcggccgca tcgtcaacgc ggatgggaag cgtgacgagc tcgttcgcgt
     3181 gcgcgagatg ctcggaatcg atcgctcgca ggtcatcgcc atcggcgacg gtgcgaa
//
LOCUS       DASURG010000347         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1081525, whole genome shotgun sequence.
ACCESSION   DASURG010000347 DASURG010000000
VERSION     DASURG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081525"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFY80_06085"
     CDS             <1..747
                     /locus_tag="VFY80_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6007511.1"
                     /translation="ATSAGIDGYLPLDWLPRRAAFTNNRGAHGAQAEEYMRMAYTLLH
                     TRMPEIIANQRAHRWAQLFSPALAGRTALVVGLGDLGEAAARAARQLKLRVVGVRRTA
                     RKSPHADAVHPYSSLDRLLPTADFVVLAVPLTPKTRNLLSRERLDRLKPTAGVINISR
                     GPVADYAALVQKLRHGRIAGAVLDVVEPEPLPPDSPLWDVPNLIITPHISCDDGERYV
                     DISLDLWFENFARYLRKKPLLRRVNRRLGY"
     gene            762..1682
                     /locus_tag="VFY80_06090"
     CDS             762..1682
                     /locus_tag="VFY80_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6007512.1"
                     /translation="MRAFEKQWPPRGKINLDHVGHFVPNMDAASTALTRLGFTVTPFS
                     AHSHRLDPGAPLLPAGTGNRCVMLKTGYLEIVTPTGDTPMADQLRLAIGRYVGIHLIA
                     FGTAAPELDHARLAAAGYAPLVPVALQRAIEVDSGTDTVRFTVVRVPPGVMPEGRIQF
                     CRQDTPHLLWQERWLDHPNRAIALTGVLLCVDEPRTAAERYGRFCGIEPEKTSSGWLI
                     QTSRGSLVLFDAETCRRTFGIVPPAMPWIAGYILATDDMNAAREHCVAAGVDPRNFVN
                     DRLLVPMPAAVGGLLIFERPESGPLELTSA"
     gene            1736..2599
                     /locus_tag="VFY80_06095"
     CDS             1736..2599
                     /locus_tag="VFY80_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX6007513.1"
                     /translation="MDRTETGMDTSVYRHIGVQPVAGALGAVIDGVHLDAALDDAVIA
                     EIRRALLKHLVVFFRGQRLAPAQQLEFARRFGEPVEYPQLKGLPECPLITPVVKLEHE
                     RVNFGGVWHSDTTYLERPPMASMLYAVEVPPYGGDTLFANQHLAYEALSDGLRRTLAG
                     LRAVNTSTKADVSRTREERMREAGAELKALSGVHPVVRTHPETGRKALYVNAGHTSHF
                     EGWSEVESRPLLEYLFAHQVRPEFTCRFRWEGGSLAFWDNRCAQHNPVNDYHGFRRVM
                     HRVTLAGDAPR"
     gene            complement(2611..>3039)
                     /locus_tag="VFY80_06100"
     CDS             complement(2611..>3039)
                     /locus_tag="VFY80_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6007514.1"
                     /translation="SYWVGRYLDRDTVRRIAGDKLNRVSEVLRLRGLLAVTAVRFVPL
                     APFAVESLVAGAIRIKAWHLLVGTFLGMLPGTLTATVFGDQIQTALKDPARINYWVVS
                     AVVIFMVAATLAVRRWLYRTQLKEHRPARGREPRPRHASS"
BASE COUNT          523 a         1021 c          958 g          537 t
ORIGIN      
        1 gcaacctcgg ccggtatcga cggctatctg ccgctcgact ggctgccgcg ccgtgccgca
       61 ttcaccaaca accggggggc gcatggtgca caagccgagg agtacatgcg catggcctat
      121 accctgcttc acacccgcat gccggaaatc atcgcgaatc agcgggccca ccgctgggcg
      181 caactcttct ccccggcgct cgccggacgc actgcgctcg tcgtcggcct cggagatctc
      241 ggggaagccg ctgcgcgcgc agcaaggcag ctgaagctgc gggtagtcgg cgtgcggcgt
      301 acggcacgca agtcgcctca tgccgacgcg gttcatccct actccagtct cgatcgtctt
      361 ctgccgaccg cagacttcgt cgtgctcgcg gtccccctca ccccgaaaac gcgcaatctg
      421 ctgagccgcg agcgcctcga tcgattgaag ccaacggcgg gcgtcatcaa catttcgcga
      481 ggaccggtcg ccgattacgc cgcgctcgtc cagaagctga ggcacggccg gatcgcgggc
      541 gcggtactcg atgtcgtcga gcccgagcct ctgccgcccg actcgccact gtgggatgtg
      601 ccgaacctca tcatcacgcc gcacatctcg tgcgatgacg gcgagcgcta cgtcgacatc
      661 tcgctcgacc tgtggttcga gaattttgcg cgctacttgc gcaagaagcc gctcctgcgc
      721 cgcgtgaatc ggcggctcgg atattaggca cgcttaccgc catgcgcgct ttcgagaaac
      781 agtggccgcc gcgtggaaaa atcaaccttg atcacgtcgg acacttcgtg ccgaacatgg
      841 atgcggcgag caccgccctg acgcgcctgg gttttacggt gacgccgttc tcggcgcact
      901 cgcaccgcct cgaccccggc gcgccgctct tgcccgcggg cacgggcaat cgttgcgtga
      961 tgctgaaaac gggctatctc gagatcgtca cgccgaccgg cgataccccc atggcagacc
     1021 aactgcgcct tgcgatcggg cgctacgtgg gaatccatct gatcgcgttc ggcacggcgg
     1081 ctccggagct tgatcatgcg cggctcgccg cggcaggcta cgcgccgctc gtccccgtgg
     1141 cactccagcg cgcaatcgag gtggattcgg gtaccgatac ggtgcggttc acggtggtgc
     1201 gcgtcccacc cggcgtcatg ccggaaggcc gcatccagtt ctgccgccag gacacgccgc
     1261 atcttctttg gcaggaacgc tggctcgacc atcctaatcg cgccatcgca ctgaccggtg
     1321 tgctgctgtg cgtcgatgaa ccccgcacgg ccgcggagcg ctatggccgc ttctgcggca
     1381 tcgagcccga gaagacgagc agcggatggc tgatccaaac gtcgcgcggc tcgcttgtac
     1441 tcttcgatgc tgagacatgt cgtcgcacat tcggaatcgt gccacccgcc atgccgtgga
     1501 tcgcaggtta cattctcgcg acggacgata tgaacgccgc acgcgagcat tgcgtggcag
     1561 cgggcgtgga tccgcggaat ttcgtcaacg atcgcctcct cgtgcccatg cctgccgcag
     1621 tcggcggatt gctcatcttc gagcggccgg aaagcgggcc gctggagctc acatcagcct
     1681 agggcttctg gcaatgggta ttcaatcgct ataggattcg ttcggaaagg tacgaatgga
     1741 tcggacagaa acgggaatgg acacgagcgt ctatcgccac atcggcgtgc agcctgtcgc
     1801 gggtgcgctc ggcgcagtaa tcgacggcgt ccatctggac gcggccctgg acgatgcggt
     1861 gatcgccgaa atccggcgcg ctctccttaa gcatctcgtc gtctttttcc gtggtcagcg
     1921 ccttgcgccc gcgcaacagc tcgaatttgc gcggcgcttc ggcgagcccg ttgagtatcc
     1981 gcaattgaag ggcttgcctg aatgcccgct catcacgccg gtcgtcaagc tcgagcacga
     2041 gcgcgtcaat ttcggcggag tctggcacag cgataccact tacctcgaac ggccgccgat
     2101 ggcgagcatg ctttatgcgg tcgaagtgcc gccttacggg ggcgatacgc tgtttgccaa
     2161 tcaacacctc gcgtacgagg cgctttccga cggattgcgc cgcaccctcg ccggcttgcg
     2221 ggccgtgaac acgtcgacca aggctgacgt gtcgagaacg cgtgaagagc gcatgcgcga
     2281 agcgggcgcg gagctgaaag cgttgtcggg cgtccaccct gtagtgcgca cgcatcccga
     2341 aaccgggcgc aaggcactct atgtcaacgc cggccacacc tcgcatttcg agggctggag
     2401 cgaagtggaa agccggccac tgctggaata tctcttcgcg catcaggtgc ggcccgaatt
     2461 cacctgccgc tttcgctggg aggggggctc gctcgcgttt tgggacaatc gctgcgctca
     2521 gcacaatccg gtcaacgact accacggctt tcgtcgcgtc atgcaccgcg taacgctcgc
     2581 gggtgatgcc ccgcgctgag aggccgcgaa tcagctgctc gcgtgacggg gacggggctc
     2641 ccgcccgcgc gccggccggt gttccttcaa ctgcgtacgg tagagccagc gtcgaacggc
     2701 gagtgtggcc gcgaccatga agatgaccac ggcgctcacc acccagtagt tgatgcgcgc
     2761 cgggtccttg agcgcggtct ggatctggtc gccgaacacc gtcgccgtca gcgtgccggg
     2821 cagcatcccg agaaacgtgc ctacgaggag atgccaagcc ttgatccgta tggcgccggc
     2881 gaccaggctt tccacggcaa acggcgcgag cggaacgaac cgcaccgccg tgaccgcgag
     2941 gagcccgcgc agccgcagca cctcgctcac gcggtttaac ttgtcgcccg cgatccggcg
     3001 caccgtgtcg cggtcgaggt agcggcccac ccagtagct
//
LOCUS       DASURG010000348         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1389115, whole genome shotgun sequence.
ACCESSION   DASURG010000348 DASURG010000000
VERSION     DASURG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389115"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFY80_06105"
     CDS             <1..268
                     /locus_tag="VFY80_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6007515.1"
                     /translation="AGTVMLATYLLVERYSRPLQLLRESMEEIGYGRFDVRIAEQRND
                     EIGELFRQFDAMAASLEERSRGDAVPARKPTHAVTRAEAGGSHA"
     gene            268..1302
                     /locus_tag="VFY80_06110"
     CDS             268..1302
                     /locus_tag="VFY80_06110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6007516.1"
                     /translation="MRRAIVTSAFAMALFMGGCASVPPPEPRAATEPSEPTEREPQHG
                     VIARSDRFVIYAPGPNDTLAELAQRFLGDAALEWRIADFNGIAAAKPGSVLTIPLKPL
                     NPMGVSSEGFQTVPVLTYHRIGGRTNRMELTARVFAAQMEYLARNGYRVVHLSELADF
                     LAGKRDLPKRAVVLTFDDGYASTYEHAFPVLKKYGFPATVFLYTNFVGAKDALTWPQI
                     REMSQSGLIDFQPHSRTHANLIVRLPGESEQRYRERLDEEIRAPRDLIRRNLSRAVSS
                     YAYPYGDANEIVLERLAQAEFRLGLTVNPGGNAFFADPLMLRRTMIFGDRGLDAFKAA
                     LQVFRETKRR"
     gene            1428..1787
                     /locus_tag="VFY80_06115"
     CDS             1428..1787
                     /locus_tag="VFY80_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007517.1"
                     /translation="MAETAETTYRERAEHHAQKGHWAEALVYWELLTLLKPDSAEYRA
                     EFDRVRNHIRQTAAALLHAASSARRRGELDHSVELYIKVLSMEPDNAAAADALRQIER
                     QRIRRAYLNRPPRLTLQ"
     gene            complement(1800..2666)
                     /locus_tag="VFY80_06120"
     CDS             complement(1800..2666)
                     /locus_tag="VFY80_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX6007518.1"
                     /translation="MNHFLKVVFGSVLIAGCATPPEPVKEAEPAVPAEQESPPAAEPG
                     PVPPKAEVKPLLTGPAARAEAQKRVRHAFELLNDGNEESASSELKYALELEPDNKAAQ
                     CLHRGITADPVAILGRESTSYVVRPGESLGRIAQRALGDVCEFYILARYNQLKVPGRL
                     AAGQTIRIPGKVALAPPDAAPPPASVSSPPPAPAPKPAVEAPAASVPPASAPAAAPSE
                     GKTPRADDAAAKNALIQRHHRNAQAAFRRQDLTTAIREWGRVLEIDPSNELAKVRREE
                     ALDLARRLKQIR"
     gene            2853..3995
                     /locus_tag="VFY80_06125"
     CDS             2853..3995
                     /locus_tag="VFY80_06125"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX6007519.1"
                     /translation="MADLPKYSTRPDDDRTTIVMRPSGLTTTAVEPIHYLVVAEGPEH
                     GRIVELGPAPLTIGRAAPSDVVLRDLELSRVHCKVQLERDAVIVVDLGSTNGTFVDGN
                     RVSGPSALARGSFLRVGRHALKYDRGTPGELKENRDFDRSLEGAARYIRSLLPAPLPT
                     GPVRTEWLLLPSKRLGGDAFGYHPIGENGFALYLFDVCGHGIDAAMLAVAVMNALRPH
                     ALAQCDFAQPAEMLRRLNGMFQMDAHGSMYFTMWYGFFDAGQRILHYASAGHHPAYLV
                     PADRGESIPLWTRNLAVGITPDYAFRSESMEVPPSSTLYLFSDGVYEIDTADGRRWDL
                     DDFIPLLAHPRVAGLTEPQRLYQAVQQVARRGPLEDDFTLLAVDFA"
     gene            complement(4002..4427)
                     /locus_tag="VFY80_06130"
     CDS             complement(4002..4427)
                     /locus_tag="VFY80_06130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6007520.1"
                     /translation="MNPIEETIPAAPSGLRRAHKMVGELQRTRNLSREVVFALNVVLD
                     ELISNIIKYAYSDEACHAIRLKLSANADFVEIQIEDDGKPFNPLRAPAPDLTSSLSGR
                     PVGGLGVHFVRSLMDAMEYARLGPLNRLTLRKRLHASKA"
     gene            complement(4485..>4752)
                     /locus_tag="VFY80_06135"
     CDS             complement(4485..>4752)
                     /locus_tag="VFY80_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX6007521.1"
                     /translation="VDDVVVIDATRYGSSARYINHSCRPNCEAIDEDGRIFIEALRNI
                     RPGEELTYDYNLVLEERHTPALKRAHPCYCGAPRCRGTLLGRKR"
BASE COUNT          834 a         1539 c         1491 g          888 t
ORIGIN      
        1 ggctggcacg gtcatgctgg cgacctatct gctggtggag cgttactcgc gaccgctgca
       61 gctcctgcgc gagtcgatgg aagaaatcgg ctatggccgc ttcgacgttc gcatcgccga
      121 gcaacgcaac gacgagatcg gcgagctctt caggcaattc gacgcgatgg cggcaagctt
      181 ggaagagcgc tccagaggcg atgccgtacc ggcgcgcaag cccacgcacg ccgtcacgcg
      241 agcagaggca ggcgggtcgc acgcgtgatg cgccgcgcga ttgtcacgag cgccttcgcg
      301 atggcgctct tcatgggggg atgcgcgagc gtacctccgc cggagccgcg agccgcgacc
      361 gagccctccg aacccacgga gcgcgagccg cagcacggcg tgatcgcccg cagcgatcgc
      421 ttcgtgattt acgcgccggg gccgaacgat acgctggcgg agctcgcgca acgctttctc
      481 ggtgacgcgg cgctcgagtg gcggatcgcc gacttcaacg ggattgccgc cgccaaaccg
      541 ggcagcgtgc tcaccattcc gctcaagccg ctcaatccca tgggcgtttc gtccgaggga
      601 tttcagacgg tgcctgtcct gacgtatcac cgcatcggcg gtcggactaa ccggatggag
      661 ctcacggcac gcgttttcgc agcgcagatg gaatatctcg ctcgcaacgg ctaccgtgtc
      721 gtccatctga gcgagcttgc cgatttcctg gcgggtaaaa gagatctgcc gaaacgcgcc
      781 gtcgttctga cgttcgacga tgggtatgcg tcgacctacg agcacgcttt tcccgtgctc
      841 aagaagtacg ggtttcccgc aaccgttttt ctctacacga atttcgtcgg cgcaaaagac
      901 gctctcacgt ggccgcagat tcgggaaatg tcgcagtccg ggctgatcga tttccagccg
      961 cattcgagga cgcacgccaa tctcatcgtg cgcttgcccg gagagagcga acagcgctac
     1021 cgcgagcgtc tggacgaaga gatccgggcg ccccgcgact tgatccggcg caatctttcc
     1081 agagcggtaa gctcgtatgc gtatccctac ggggatgcca acgagatcgt gctcgagcgc
     1141 ctggcacagg ccgaattccg gctcggcttg accgttaatc ccggcgggaa cgcctttttc
     1201 gcggatccgc tgatgctacg ccgcacgatg atcttcgggg atcgcgggtt ggacgctttc
     1261 aaggcggcgc tgcaagtgtt tagagagacc aagcgccgat gacaggaaaa cagggagcac
     1321 tgctcgcgtc tctcgcagcg gcatggatcg ctggctgcgc cacgccacca ccggacccga
     1381 ccgccgagac gccgcgcgtg gctgtgctgc cggctccgca agcgagcttg gcggagacag
     1441 cagagacgac atatcgcgag cgcgcggagc accacgctca gaaaggacac tgggccgaag
     1501 cgctggtgta ctgggaactg ctcacactgc tcaaacccga ctccgcggag taccgggccg
     1561 aattcgatcg tgtccgcaac catatccggc agacggcagc cgctctgctg cacgcagcat
     1621 cgagcgcccg caggcgcggc gaactcgacc attctgtcga gctctacatc aaagtgttga
     1681 gcatggagcc ggataatgcg gctgccgccg acgctctgcg gcaaatcgag cgtcagcgga
     1741 tacggcgcgc ctacctcaac cggccgcccc gtctcaccct tcaataacct cgggtgcgcc
     1801 tacctgatct gcttcaggcg gcgcgccaga tccagcgctt cctcccggcg cactttggcg
     1861 agctcgttcg acgggtcaat ctccagcact ctcccccatt ccctgatcgc ggtggtgagg
     1921 tcctgccgcc tgaaagcagc ctgcgcgttg cggtggtgac gctggatgag cgcgttcttc
     1981 gccgctgcat cgtctgcacg cggtgtcttg ccctcgctcg gtgctgctgc tggtgcactg
     2041 gctggcggca cggaagccgc aggggcttcc accgcgggtt ttggagcagg tgccggcggc
     2101 ggtgacgaca cgctggctgg tggcggtgcg gcgtcaggcg gcgcgagcgc caccttgccg
     2161 gggatccgga tggtctggcc cgctgcaagg cgcccgggga ctttgagctg gttatagcgc
     2221 gcgagaatat agaactcaca gacgtcgccg agcgctcgct gcgcaatgcg gcccagactt
     2281 tcgccgggcc gcacaacata gctcgtcgat tcccggccca agatcgccac ggggtctgcc
     2341 gtaatgccgc gatggagaca ctgcgccgcc ttgttgtccg gctcgagctc cagggcatac
     2401 ttcagttcag agcttgcgct ttcttcgttg ccgtcgttca gcaattcgaa ggcgtggcgc
     2461 acccgcttct gcgcttccgc ccgcgcagcc gggcccgtca gcagcggctt cacctctgcc
     2521 ttgggcggta caggtccggg ttccgctgca ggtggcgatt cctgctcggc ggggacggcc
     2581 ggctcggctt ccttgaccgg ctccgggggc gtcgcgcatc cggcaatgag caccgatccg
     2641 aagaccacct tgagaaagtg attcacggca gagccaaaaa tccggtgttc tccgtcactt
     2701 ggggagggct gagacaattc agcacggcat catacaggaa ggcgataatt caccacaatt
     2761 cgatatccgg cgagcctgcg gttcacggag cagcttgcgt aagcatccgc catgacttac
     2821 gctcgaattc ccgatactgg cgcgcaaaca ctgtggccga cctcccaaaa tactctacgc
     2881 gccccgacga cgaccgcacg acgatcgtga tgcgaccgag cgggctcacc accactgcgg
     2941 tcgaacccat acactacctc gtcgttgcgg aaggccctga acacgggcgc attgtcgaac
     3001 ttggacccgc tccactcacg atcggacgtg cagccccttc cgatgtcgtg ttgcgtgacc
     3061 tcgaattgtc acgcgtccat tgcaaggtac agctcgagcg tgatgccgtc atcgtcgtcg
     3121 acctcggctc cacgaacggc acattcgtcg acggcaatcg tgtgagcggc ccctctgctc
     3181 ttgcgcgagg cagcttcctg cgcgttgggc ggcacgcgct caagtatgat cgaggcaccc
     3241 ccggcgagct taaggaaaac cgtgactttg accgcagcct tgaaggcgct gcccggtaca
     3301 tccgctcgct cctgcccgcc cccctgccca caggcccggt ccgcaccgaa tggctgctgt
     3361 tgccctccaa acggctgggc ggtgacgcct tcggttacca cccaatcggc gaaaacggtt
     3421 ttgcgttata cctcttcgac gtttgcgggc atggcatcga cgccgccatg ctggcggtcg
     3481 cggtgatgaa cgcattgcgc ccgcatgcac tggcgcagtg cgatttcgcg cagccggcgg
     3541 agatgctgcg caggctgaac ggcatgttcc agatggacgc ccacggctcg atgtacttta
     3601 cgatgtggta cggtttcttt gatgccggcc agcggatatt gcactacgca tcggccggcc
     3661 atcatccggc atacctcgtg cccgcagacc gcggcgaatc gataccgctc tggacccgca
     3721 atctggcggt gggcatcacc cccgattatg cgttccgctc ggaatcgatg gaggtgccgc
     3781 cgagcagtac cctttatcta ttcagcgatg gtgtctacga gatcgacacg gccgatggcc
     3841 gccgctggga tctcgacgat ttcatccctc tgcttgcgca tccccgcgta gcgggattga
     3901 cggagccgca gcgactctac caagcggtac agcaggtcgc gcgccgcggc cccctcgaag
     3961 acgacttcac gctgctcgcg gtcgacttcg catagcggac ttcacgcctt cgaggcgtgc
     4021 agccgtttcc tgagcgtcag gcggttgagc ggtccgaggc gcgcatattc catcgcgtcc
     4081 atcaagctgc gcacgaagtg cacacccagt cctccgacgg ggcgtcctga cagcgaagat
     4141 gtgagatctg gcgccggcgc cctgagcgga ttgaacggct tgccatcgtc ctcgatttgg
     4201 atctcgacaa aatcggcgtt tgcggagagc ttgagccgga ttgcgtggca cgcctcatcc
     4261 gagtaggcat atttgatgat gttgctgatc aattcgtcga gcaccacgtt gagtgcaaag
     4321 accacttcac gcgacagatt tcttgtgcgc tgcagctcac cgaccatctt gtgggcgcgg
     4381 cgaagtccgc tcggcgcggc gggaatcgtc tcttcgatcg ggttcacgcg ggacgtggga
     4441 aatggtagcg agcgcgctcg ttgtcccgtc ggcctgcgag acgatcaacg tttgcgaccg
     4501 agaagcgtgc cgcgacagcg cggcgcgccg caataacacg ggtgcgcgcg cttcagcgcc
     4561 ggcgtatgac gctcttcgag cacgaggttg tagtcgtaag tcagctcttc gcccggccgg
     4621 atgttgcgca gcgcctcgat gaaaatgcgg ccgtcttcat cgatcgcctc gcagttcggg
     4681 cggcaggagt gattgatgta gcgggcggag ctgccgtagc gggtggcgtc gatcacgacc
     4741 acgtcgtcca cc
//
LOCUS       DASURG010000349         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_980057, whole genome shotgun sequence.
ACCESSION   DASURG010000349 DASURG010000000
VERSION     DASURG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980057"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1625
                     /gene="pyk"
                     /locus_tag="VFY80_06140"
     CDS             198..1625
                     /gene="pyk"
                     /locus_tag="VFY80_06140"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX6007522.1"
                     /translation="MLRRTKIVATLGPASRDPKVLGRMIQAGLDVVRVNFSHGSAAEH
                     RRLVGLVRTLARKSGRAVGVLVDLQGPKIRIGRFRDGRITLGAGAKFVLDADCDMGDQ
                     QRVGLDYRNLPNDVRPGDTLLLDDGRVVLGVSTVNGPRITTVVEQGGVLSNNKGINRK
                     GGGLTAPALTEKDMQDIKVAAELKADFLGVSFPRSGADIQWARDLMEKAGGRALMIAK
                     IERAEAVPALDEILDASDGIMVARGDLAVEVGDAAVPALQKRMIRMAREKNRVTITAT
                     QMMESMISSPIPTRAEVSDVANAVLDGTDAVMLSAETATGLYPAETVAAMARVCVEAE
                     KEDTFSPERRHASGPAEVEEAVARATMFTSSNLDIKAIAAMTQSGKTVLLMSRMHSPV
                     PIYAMSAVVETRRRVTLFRGVHPIKFKGVRDSERSLHMAEDELLARGAVHNGDYIVLT
                     IGEPWGKAGGTNTLKIVKVGEHRHA"
     gene            1739..>2617
                     /locus_tag="VFY80_06145"
     CDS             1739..>2617
                     /locus_tag="VFY80_06145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX6007523.1"
                     /translation="MSAHQMATVAQAMVAPGKGILAADESTGTIEKRFNSIKVESTEE
                     NRRAYRDMLFSARGLHQYVSGVILYDETIRQKASDGTPFVELLSRNGILPGIKVDTGA
                     KALALCPGETVTEGLDGLAKRCAEYVKMGAKFAKWRAVITIGAGLPTPTCIAANAHAL
                     GRYAAICQEAGLVPIVEPEVLMDGDHTIDRCEEVTEWTLNAVYDELYRNRVTLEQSVL
                     KPSMVISGKSCLNQAGVEEVAERTVRILKRTVPAAVAGIVFLSGGQSDEVATQHLNAM
                     NRMFVGKLPWPLSFSYG"
BASE COUNT          506 a          814 c          875 g          422 t
ORIGIN      
        1 gaccgaattt cgtacatatc cacgggaggc ggcgcgtttc tcgaattcct cgagggcaag
       61 aagctgcccg cgatcgaaat tctggagcag cgcggccgct agctagccca gccgcgcgtt
      121 gagtttgcgg cggccggccg ctagactctg cgcaaaacgg caggcaacgt cccctctcag
      181 gcgcaaacgc ttttatcgtg ctccgccgca ccaagatcgt cgccacgctc ggccccgctt
      241 cacgtgatcc caaagtgctg gggcgcatga tccaggcggg gctggatgtc gtccgcgtca
      301 atttctcgca cggcagcgca gccgagcatc gccgcctcgt ggggctcgtg cgcacgcttg
      361 cacgcaagtc gggtcgcgcg gtgggcgtgc tcgtcgatct gcagggaccg aagatccgca
      421 tcgggcgctt ccgcgacggc cggatcacgc tcggagccgg ggcgaaattc gttctcgacg
      481 ccgattgcga tatgggcgat cagcagcgcg tcggcctgga ctaccgaaat cttccgaacg
      541 acgtgcggcc aggggacacc ctgctcctcg acgacggccg cgtcgttctg ggggtatcca
      601 cggtgaacgg tccgcgcatc accacggtcg tcgagcaggg cggcgtgctt tcgaataaca
      661 agggcatcaa tcgcaagggc ggggggctta cggcgccggc actgactgaa aaggacatgc
      721 aggacatcaa ggttgcggcc gagctgaaag cggattttct cggtgtctcg tttccgcgct
      781 cgggtgcgga catccagtgg gcgcgcgacc tcatggagaa agcgggcggt cgcgcactca
      841 tgattgcaaa gatcgagcgc gccgaggcgg tgccggcgct cgatgaaatt ctggatgcct
      901 cggacggcat catggtcgca cgcggtgatc tcgctgtgga agtgggcgac gccgcagtgc
      961 cggcgctcca gaaacgcatg attcggatgg cgcgcgagaa gaacagggtg accattaccg
     1021 cgacacagat gatggaatcc atgatctcga gcccgatccc cacgcgcgcc gaagtgtctg
     1081 acgtcgcgaa cgcggtgctc gacggaaccg acgcggtcat gctttcagcc gagacggcga
     1141 cgggactcta cccagccgaa accgtggcgg cgatggcgcg cgtctgcgtc gaggcggaaa
     1201 aggaggatac gttcagcccg gagcgccgcc atgcgagcgg gccggccgag gtggaggaag
     1261 cggtcgcacg agccaccatg ttcacctcca gcaacctcga tatcaaggcg atcgcggcga
     1321 tgacacagag cggaaagacg gtgctcctga tgtcgcgcat gcattcgccg gtgccgatct
     1381 acgcgatgag cgcggtcgtc gagacgcggc gccgggtgac gctcttccgc ggcgttcatc
     1441 cgatcaagtt caagggcgtc cgggactcgg agcgctcgct ccacatggcg gaggacgaac
     1501 ttcttgcgcg cggcgccgtt cacaacgggg actacattgt gctgacgatc ggcgagccct
     1561 ggggcaaggc gggaggcacc aatacgctca agatcgtaaa ggtcggcgag catcgccatg
     1621 cgtaaattac ttcggatgat ccgaacgggg cgtcgggccc cgcgagcggc cccgtagtaa
     1681 aatagccggg cactgtctat cgtccatcgt tgtactccct agcgaagcag gaggttctat
     1741 gtcagcacat caaatggcca ccgtggccca ggcgatggtc gccccgggca aaggcatact
     1801 ggctgccgac gagagcaccg gcaccatcga gaagcgcttc aacagtatca aagtcgagag
     1861 cacggaagag aatcgccgcg cctatcgcga catgctcttc agcgcgcgcg gcctccacca
     1921 gtacgtgagc ggcgtcatcc tctacgacga aacgatccgc cagaaggcgt cagacggcac
     1981 gccgttcgtg gagctgcttt cccgcaacgg catactcccc gggatcaaag tcgataccgg
     2041 cgccaaggcg ctcgcgctct gcccgggcga gacagtgacc gagggtctgg acggtctcgc
     2101 caaacgctgc gccgagtacg tgaagatggg cgcgaagttc gccaagtggc gcgccgtcat
     2161 caccatcggt gcgggcttgc ccacgccgac ctgcatcgca gcgaacgcac acgcgctcgg
     2221 acgctacgcg gcgatctgcc aggaagcggg cctcgtgccg atcgtggagc cggaagtgct
     2281 gatggacggc gaccacacga tcgatcgctg cgaagaggtc acggagtgga ccctgaacgc
     2341 cgtgtacgac gagctctacc gcaaccgtgt gacgctggag cagtcggtgc tcaagccaag
     2401 catggtgatc tccggcaaga gctgccttaa ccaggcgggg gtggaagaag tcgcggagcg
     2461 caccgtgcgc attctgaagc gcaccgtgcc ggcggccgtt gcgggcatcg tgttcctgtc
     2521 cggcggccag agcgacgaag tggcgaccca gcacctgaat gccatgaacc gcatgttcgt
     2581 gggcaagctg ccgtggccgc tctctttctc gtacgga
//
LOCUS       DASURG010000350         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_877375, whole genome shotgun sequence.
ACCESSION   DASURG010000350 DASURG010000000
VERSION     DASURG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877375"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="pyrB"
                     /locus_tag="VFY80_06150"
     CDS             complement(<1..435)
                     /gene="pyrB"
                     /locus_tag="VFY80_06150"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX6007524.1"
                     /translation="MWLHPSNPQLNRNGELHHLLSIEGLPAEILWQILDTAQSFVGVT
                     QREVKKVPLLRGKAVFNLFFEPSTRTRTTFEIAAKRLSADVINLNIAASSQTKGESLL
                     DTVANLSAMQADIFVVRHASSGAPFLIARHVKPHEHVVNAGDG"
     gene            complement(438..947)
                     /gene="pyrR"
                     /locus_tag="VFY80_06155"
     CDS             complement(438..947)
                     /gene="pyrR"
                     /locus_tag="VFY80_06155"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6007525.1"
                     /translation="MPGKPLPDAEALLAALTEKMRPVVGSDSGLIGIHTGGVWLAERL
                     HSALGLKVPCGTLDVSFYRDDYEKIGLHRNVKASAVPFEVDGRHLVLVDDVLYTGRTI
                     RAAMNEIFDYGRPASIRLAALVDRGGRELPIAAQFVGATLELAAGESIELTRDANGLL
                     KLVLHRGHP"
     gene            complement(983..1417)
                     /gene="ruvX"
                     /locus_tag="VFY80_06160"
     CDS             complement(983..1417)
                     /gene="ruvX"
                     /locus_tag="VFY80_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX6007526.1"
                     /translation="MIAFDFGEKRIGSAVGDLALRIAHPLTTVEAEDNRSRFAAIAKL
                     IDEWRPVVLVVGVPVPSDPGEHEIARLARRFARRLEGRFGIRTDLIDERLSSHAAEAS
                     LREQGVRGRRMKNVVDAAAAQQILQSYFETLRVRVSSAAHRP"
     gene            complement(1524..2084)
                     /locus_tag="VFY80_06165"
     CDS             complement(1524..2084)
                     /locus_tag="VFY80_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX6007527.1"
                     /translation="MQKVNLTQHFLIAMPNMVDPHFARTLTFICEHNEQGALGVVVNR
                     PIDMTLEALLEQISVPLESDVFKSLPVHFGGPVQVDRGFVLHTPIGEWQSTLAVGSDI
                     GLTTSKDILQAVARGAGPRQILVTLGYAGWAPGQLEHELSQNAWLTVQAKPEVIFELP
                     SEERLPAAMSLLGVDYATLSEQAGHA"
BASE COUNT          422 a          721 c          734 g          396 t
ORIGIN      
        1 gccgtcgccg gcgttgacca cgtgctcgtg cggcttcacg tggcgcgcga tcaggaacgg
       61 cgcccccgag gatgcgtggc gcacgacgaa gatgtccgcc tgcatcgccg agaggttcgc
      121 cacggtgtcg agaagcgact cgcccttggt ctgcgaggaa gcggcgatgt tcaggttgat
      181 cacgtccgcg gacaggcgct tcgccgcgat ctcgaacgtg gtgcgcgtgc gggtcgaagg
      241 ctcgaagaag aggttgaaca ccgcctttcc gcgcagcagc ggcactttct tcacttcacg
      301 ctgcgtgacg ccgacgaacg actgcgcagt atcgagaatc tgccatagaa tctccgcggg
      361 gagcccctcg atcgacagca gatggtgcag ctccccgttc cggttaagtt gcggattcga
      421 gggatggagc cacatgacta agggtgccct cgatgcagta cgagcttcag caacccgttg
      481 gcatcgcgcg taagctcgat gctttcgccc gccgcaagct cgagcgtcgc ccccacgaac
      541 tgagcggcga tcggaagctc gcgcccgccg cggtccacca gcgcggcaag ccggatgctc
      601 gcggggcggc cgtagtcgaa gatctcgttc atcgccgcgc gtatggtgcg cccggtgtaa
      661 agcacatcgt ccaccagcac gagatgccgg ccgtccactt cgaagggaac ggcggaggcc
      721 ttcacgttgc ggtgcaggcc gatcttttcg taatcgtcgc gatagaagga gacgtcgagc
      781 gtaccgcagg gcactttgag gccgagagcg gaatgcagtc gctcggcgag ccagacaccg
      841 ccggtgtgga tgccgatcaa gccgctgtcg gacccgacca cgggtcgcat tttctcggta
      901 agcgcagcga ggagcgcctc ggcgtcgggt agcggtttgc ccggcatggc ggcgcgtaca
      961 ctaccacaac ttggaagcct gcttaagggc ggtgtgccgc gctcgaaacc cttacccgta
     1021 gcgtctcgaa atagctttgc aggatctgct gtgcggctgc ggcatcgacc acgttcttca
     1081 tgcgcctgcc gcgcactccc tgctcgcgca gcgacgcttc cgccgcgtgg gagctgagtc
     1141 gctcgtcgat caggtcggtg cggatgccga agcgtccttc gagccggcgg gcgaagcgcc
     1201 gcgcgagccg cgcgatttcg tgctcaccgg gatcggacgg aacgggaacg ccgacgacca
     1261 gcaccaccgg acgccattcg tcgatcaatt tcgcaatggc cgcaaaccgg ctccgattgt
     1321 cttccgcttc cacggtagtg agcggatggg caatcctcag agcgagatcg cccacggcgg
     1381 aaccgatgcg cttctcaccg aagtcgaaag cgatcacggt accacgagcc ggtgtctgga
     1441 gcgcgtatgt cgggacggga ctcaatgcgc cgagtgtgag cgtgcggaag gcgcgcgtgc
     1501 ggccgccacc acgcgtagcg tgtttacgcg tgacccgcct gctcggaaag cgtcgcgtag
     1561 tccacgccga gcaggctcat ggcggcgggt aacctctctt cggacggcag ctcgaaaatg
     1621 acttccggct tcgcctgcac ggtgagccag gcattctgcg atagctcgtg ctcgagctgc
     1681 cccggcgccc atcccgcata ccccagcgta acgagaatct gacgcggccc tgcgccgcgc
     1741 gcgacggcct gcaggatgtc cttggacgtc gtgagcccga tgtccgagcc gacggccaag
     1801 gtcgattgcc attcaccgat tggcgtgtgg agcacgaacc cgcggtccac ctgcaccggg
     1861 ccgccgaagt gcacgggtag cgacttgaag acatccgatt cgagcggaac actgatctgc
     1921 tcgagcagtg cttcgagcgt catgtcgatc gggcggttca ccaccacgcc gagcgcgcct
     1981 tgctcgttat gctcgcagat gaaggtcagt gtccgggcga aatgcggatc caccatgttc
     2041 ggcatggcga tcaggaagtg ctgcgtcagg ttcacttttt gcatgcccgg ctcgcggtcc
     2101 tattgtaaac accgcagtcc gactccacaa aggaccgcca tggtaagtag cgttagcggg
     2161 ctaaaggttc aaccgctact tccgccagaa cgtcggagtg aataccacca atacggtgaa
     2221 gagttccagc cgaccgaaca gcatcgccag aacgcagatc cagagctgaa ggt
//
LOCUS       DASURG010000351         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1184672, whole genome shotgun sequence.
ACCESSION   DASURG010000351 DASURG010000000
VERSION     DASURG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184672"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFY80_06170"
     CDS             complement(<1..435)
                     /locus_tag="VFY80_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6007528.1"
                     /translation="MKPFRPGLTHTPVTPFKRDLGVDFDAFERLIEFHLRHGAEALAL
                     PLHVGESVSLTDAEQRKLLEFAIAKVNGRVPVIAHASDSGTGIAVARARHAEDVGAAA
                     VVATTPYYWTPPAGMVLEHFARIGTAVRIPFLVLYTPDEMPGT"
     gene            complement(455..1369)
                     /locus_tag="VFY80_06175"
     CDS             complement(455..1369)
                     /locus_tag="VFY80_06175"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6007529.1"
                     /translation="MAIRLKGVMQSTVTPLKEDFSPDLATFERLVEFHVRTGATAIAW
                     PHHKAESLNLTIAERKLFAEVAVKVTARRVPVSIHVSALAVEDTMELARHAEKIGADA
                     ILAITPYFWNPTEEALYDYFVRLGTSIGLPLIAYNSPSYLSGVEITGNLTRRLIERLP
                     NFIGMKEASFNSEKFTEIARAALALRPEFALIAGVEWLAPSVPLGGAGSYSSAGAICP
                     NLCSALYDACVAGEWPKARALQYKLAQLWDLFRDQYPSSLKGGMVIMGRAVGPTRPPL
                     PTASPERIAYIRTQLEELGILDTEPHGW"
     gene            complement(1380..2876)
                     /locus_tag="VFY80_06180"
     CDS             complement(1380..2876)
                     /locus_tag="VFY80_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6007530.1"
                     /translation="MLIRLAHGTPIYCTREIREIESAAANLPLMERAGLAAAELARTV
                     APESGRPILVVAGPGNNGGDAFVVARYLKAWWFKVCVVFAGEEAKLSRDATRALKAWR
                     DAGGTTLDRIPADTDWGLAVDGLFGIGLQREITGPYAEMIASLNALAVPKLAIDIPSG
                     LESDTGRVMGCAVRADHTLTFIALKPGLLTHEGPDQCGQIHVADLGLDAGVFDRPHGA
                     VLGEGVLHDALPRRRLSSHKGDYGSVAIVGGAHGMVGAALLAGRAALKLGTGRVYLGL
                     LARDAPLVDVMQPEIMVRSADEALKLGHLTALAIGPGLGQSPDAAFHLDWALDTALPL
                     VIDADGLNLIAAHDGYASKLRRRSAPTVLTPHPAEAARLLHCDTSDVQRDRLAAAARL
                     AGEFESHVVLKGAGSICTAPDGAWAINVSGNPGMASAGMGDVLTGFVSALLAQRVDTQ
                     LALRIAVHLHGAAADALVAEGTGPIGLTAGDTIAAAQRILNSRHGNTT"
     gene            2977..>3413
                     /locus_tag="VFY80_06185"
     CDS             2977..>3413
                     /locus_tag="VFY80_06185"
                     /inference="COORDINATES: protein motif:HMM:NF037184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX6007531.1"
                     /translation="MPQLIRLRGRSALSAFRLRKFLAKLAPFIPDLEFDAEYWHFVML
                     REDLEPRAHERLERILTYGPSGEPTRRDGVLCLVVPRIGTVSPWSSKATDIAHHCGLT
                     AVERIERGVAYWLSRRLSREERAALAPLIHDRMTETMLGSFDEV"
BASE COUNT          595 a         1081 c         1141 g          596 t
ORIGIN      
        1 tgttccgggc atctcgtctg gcgtgtacag gacgaggaac gggatacgca ccgctgtgcc
       61 gatccgggcg aagtgttcga gcaccatgcc cgccggcgga gtccagtagt acggagtcgt
      121 cgcaaccacg gccgccgcgc cgacgtcttc agcgtggcgg gcgcgggcaa ctgcgatacc
      181 ggtgccggag tcgctggcgt gagcaatgac gggcacgcgg ccgttcactt tcgcgatcgc
      241 gaactcgagc agcttgcgct gctcggcatc ggtgagactc accgattcgc cgacgtgcag
      301 cggaagcgcg agcgcttcgg cgccgtgacg aagatggaat tcgatcagcc gctcgaacgc
      361 atcgaaatcg acaccaaggt cgcgtttgaa cggcgtgacg ggggtgtggg tgaggccggg
      421 gcgaaagggc ttcatggtaa gtgatcctct cctttcacca gccgtggggc tcggtgtcga
      481 gtatgcccag ctcttcgagc tgggtgcgga tgtaggcgat gcgctcgggg cttgcggtag
      541 gaagcggcgg gcgcgtcggg cctacggcgc ggcccatgat caccatgccg cccttgagtg
      601 aagacggata ctgatcgcga aagagatccc acagctgcgc gagcttgtac tggagcgcac
      661 gcgctttcgg ccactctccc gcgacacacg cgtcatacaa ggcgctgcag aggttcgggc
      721 agattgcacc cgccgaggag taggaacccg cgccgccgag cggcacggaa ggcgccagcc
      781 attcgacgcc tgcgatcagc gcaaactcgg gccgcagcgc aagcgccgcg cgtgcgatct
      841 cggtgaattt ttcgctgttg aaactcgcct ccttcatgcc gatgaagttt ggcagccgct
      901 cgatgagccg ccgcgtgagg ttgccggtga tctcgacgcc cgagagatag ctcggcgagt
      961 tgtaagcgat gagcggaagt ccgatggaag tgccgaggcg cacgaagtag tcgtaaagcg
     1021 cctcttccgt cgggttccag aagtagggcg tgatcgcgag aatcgcgtcg gccccgatct
     1081 tctcggcatg gcgcgcaagc tccatcgtgt cctccaccgc gagcgcactg acgtgaatcg
     1141 acaccggcac gcggcgggcg gtgaccttga cggccacttc ggcgaagagc ttgcgctccg
     1201 cgatcgtgag attgagcgac tccgctttgt ggtgcggcca cgcgatcgcg gtggcgccgg
     1261 tgcgcacgtg aaattccacc agtcgctcaa acgtggcgag atccggacta aagtcctcct
     1321 tcagcggggt caccgtgctc tgcatgacgc ctttgagtcg tatggccatg atcctctgcc
     1381 taggtcgtgt tgccgtgtcg tgaattcaga atccgctgcg cggcggcaat tgtatcgcct
     1441 gccgtaagcc cgatggggcc cgttccctcc gcgacaagcg cgtccgccgc cgcgccgtgc
     1501 agatgaaccg ctatccgcag cgcaagttgt gtatccactc gctgcgcgag cagagcagag
     1561 acgaagccgg tcagcacatc ccccatgccc gcgctcgcca tgccgggatt gcccgatacg
     1621 ttgatcgccc atgcaccatc cggcgcggtg cagatactgc cggcgccttt cagcaccaca
     1681 tggctttcga actcgccggc gagccgcgcg gctgccgcaa gacgatcacg ctggacgtcc
     1741 gacgtgtcgc aatggagaag acgtgcggct tcggcggggt gaggcgtgag tacggtcggc
     1801 gcgctgcgcc ggcgaagttt tgacgcgtat ccgtcgtggg ccgcgatcag attgagcccg
     1861 tcggcatcga tcacaagcgg cagcgcggta tccaaggccc agtccagatg aaaggccgca
     1921 tcgggcgact gaccgagccc cggtccgatg gcgagcgcgg tgagatggcc gagcttgagc
     1981 gcttcatcgg cgctacgcac catgatttcg ggctgcatca cgtctacgag cggcgcatcg
     2041 cgggcgagga gtccgaggta gacgcgaccg gtacccaatt tgagcgccgc ccggcccgcg
     2101 agtagcgccg cgcctaccat gccgtgggcg ccgccgacga tcgcgacact cccgtagtcg
     2161 cccttgtgcg aactcaggcg ccgccgcggc agcgcatcgt gcagcacgcc ttctccaagc
     2221 accgccccgt ggggccggtc gaagacgcca gcatcgagac cgagatcggc aacgtgaatt
     2281 tggccgcact ggtccggccc ctcgtgggtg aggagtcccg gtttgagcgc gatgaaggtg
     2341 agggtgtggt ccgcgcgcac cgcgcaaccc attacgcgac cggtatcgga ctcgagcccg
     2401 ctcggtatgt cgatcgcgag cttcggcaca gcaagcgcat tgagggacgc aatcatctcg
     2461 gcatacggcc cggttatttc acgctggagc ccgatgccga agagaccatc gaccgcgagt
     2521 ccccaatccg tgtctgccgg aatgcggtcg agcgtagtgc cgcccgcatc gcgccatgct
     2581 ttgagcgcac gagtggcgtc gcgcgaaagc ttcgcttcct cgccggcaaa gacgacgcat
     2641 accttgaacc accaggcctt gagatagcgt gcaacgacga aggcgtcgcc gccgttgttt
     2701 ccggggccgg cgacgacgag aatgggccgg cccgactcgg gcgcgaccgt ccgcgcgagc
     2761 tcggctgctg cgagtccggc gcgctccatg agcggcagat ttgcggcagc ggattcgatt
     2821 tcgcggattt cccgcgtgca ataaatcggc gtgccgtgcg cgagtcggat cagcatggtg
     2881 aatgcatcgg gtggaagcgc tgcgagcacg cgtagtttaa cggaaaggca gcgcttattt
     2941 ggtaaaatgc gtttttgtcg cgtcgcccag aactgtatgc cgcaactcat ccggctgcgc
     3001 ggacgcagcg cgctttccgc cttccgactc cggaaattcc tcgcaaagct cgcccccttc
     3061 attcccgatc tcgagttcga cgctgaatac tggcacttcg tcatgctgcg cgaggacctc
     3121 gagccgcgcg cgcacgaacg gctggaacgc attctgacct atgggccgag cggcgagccg
     3181 acgcggcgcg atggcgtact ctgcctcgtg gtgccgcgca tcgggacagt ttcgccgtgg
     3241 tcgtccaagg caaccgatat cgcgcatcat tgcgggctca cggcagtcga gcgcatcgag
     3301 cgcggcgtgg cgtactggtt gagtcgccgg ctctcccgcg aggagcgtgc ggcgctcgcg
     3361 ccgcttatcc atgaccgaat gaccgaaacg atgctgggct cgttcgatga ggt
//
LOCUS       DASURG010000352         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_980363, whole genome shotgun sequence.
ACCESSION   DASURG010000352 DASURG010000000
VERSION     DASURG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980363"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /gene="tcuA"
                     /locus_tag="VFY80_06190"
     CDS             complement(<1..1433)
                     /gene="tcuA"
                     /locus_tag="VFY80_06190"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6007532.1"
                     /translation="MNGQYDVVVVGKGNAALCAALAAREHGVSVAILEAASEDESGGN
                     SRFAGGVMRFAYSTVDDLKRVTDISDEEVAGSDFGTNTREEYLDDLYRLTSYRTDPEL
                     SELLVNRSLDTMAWLRSKGVRFNLNFGRQSGLVGGKRKFFGRMPIEASGGGAGLVQNL
                     DAAAKKAGIEVFYETRAVSLLYDGERVLGLRVKQNGKPAELRAKSVVLACGGFEANPE
                     WRTRYLGPGWELCKVRGTRFNVGDGLRMALDIGAAPYGNWSGCHAVGWDRYAPEFGDI
                     NVGDQFQKHSYIFGILVNANGTRFVDEGWDFHSFTYAKYGGEVLKQPGQFAWQVFDSK
                     VKHLLRSEYRIKFVTKVTANTLEELAPQLEGVDAQAFLKTVREFNAAVKLDVPFDHTI
                     KDGKCTVGIDPPKSNWAQPLDTPPFEAYATTCGITFTFGGLRIDKESGQVLDVHFHPI
                     PGLYCAGEMVGGLFYFNYPSGTGLVSGA"
     gene            complement(1514..2503)
                     /locus_tag="VFY80_06195"
     CDS             complement(1514..2503)
                     /locus_tag="VFY80_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6007533.1"
                     /translation="MNPTEKKAQPVSETKARRMREMLASGKIVVSPGVFDGYSVRLVE
                     AMGFQAASTTGAGLSNSRLGQPDIGVMTLRDNVEACYHIARSVSIPVMSDADTGYGNA
                     VTVYHTVRYFEEAGVVGINIEDQVSPKRCGHMRGKELISAAEMAKKIESAIKARRDPD
                     FIINARTDAIAVEGPESAIERAKVYLAAGADMVYPDAIGSEDQIRRFVDAVEAPVSIS
                     MGLGLRGRVTTPLVSVKRLQAIGVARVSYSRMLTSSALKGMKNALQAFREYLDVGEPP
                     ERPDLAVSAEEISELMGYAFVNELESQFLLDEQIEKKYGGGKVDYVVRGRERT"
BASE COUNT          478 a          929 c          841 g          555 t
ORIGIN      
        1 gcccctgaca cgagtccggt accgctcggg taattgaagt aaaagagccc gccgaccatt
       61 tcgcccgcgc aataaaggcc ggggatcgga tggaagtgca catcgagcac ctgtccgctc
      121 tccttgtcga tgcgcaagcc cccgaacgta aacgtgatac cgcacgtcgt ggcatacgct
      181 tcgaacggcg gcgtgtccag cggctgcgcc cagttcgatt tcggcggatc gatgccgacg
      241 gtgcacttcc cgtccttgat ggtatggtcg aacggcacgt ctagcttcac cgctgcgttg
      301 aactcgcgca cggtcttgag gaaggcctgc gcatccacgc cttcgagctg gggcgcgagt
      361 tcctccaacg tgttcgccgt cactttggtg acgaacttga tgcggtactc cgagcgcagc
      421 aggtgcttca ctttcgaatc gaacacctgc catgcgaact ggcccggctg cttcaggacc
      481 tcaccgccgt actttgcgta agtgaaggag tggaagtccc aaccttcgtc gacgaagcgc
      541 gtaccgttcg cgttgacgag aatgccgaag atgtagctgt gcttctggaa ttggtccccg
      601 acgttgatgt cgccgaattc cggcgcgtag cggtcccagc ccacggcgtg acagccggac
      661 cagttgccgt agggcgccgc gccgatgtcg agtgccattc tgagtccgtc gccgacattg
      721 aagcgggtcc cgcgcacttt gcacagttcc cacccagggc cgaggtagcg tgtgcgccac
      781 tccgggttcg cctcgaatcc gccgcaggcg aggaccaccg attttgcacg caactcggcg
      841 ggctttccgt tctgcttcac gcgcaggccg agcacccgct cgccgtcgta gaggagcgac
      901 acggcacgcg tctcgtaaaa gacctcgatg cccgcttttt tcgccgccgc atcgagattc
      961 tggacgagcc ccgcaccgcc gccggaagcc tcgatcggca tgcgcccgaa gaacttgcgc
     1021 ttgccgccga cgagccccga ttgtcgcccg aagttgagat tgaagcgtac gcctttcgag
     1081 cgcagccacg ccatcgtgtc gaggctgcgg ttcaccagga gctccgagag ctccggatcg
     1141 gtgcggtagc tcgtcagccg gtagaggtcg tcgagatatt cctcgcgcgt gttggtgcca
     1201 aaatcgcttc cggcgacctc ttcatcgcta atgtccgtga cgcgcttcag atcgtccaca
     1261 gtactgtaag cgaagcgcat gacgccgccg gcgaaacggc tgttgccgcc cgactcgtct
     1321 tcggacgccg cttcgaggat cgcgacggag acgccgtgtt cgcgcgcggc gagggcggcg
     1381 cacagtgcag cgttgccctt gccgaccacc actacatcgt attgaccgtt cacgtgcgcc
     1441 gcctggtgaa gcttcaagcc ataatcgcga aaccagcaat tctacgacaa ctcatgcacg
     1501 cctttgcatc tgctcatgtg cgctcgcgcc cgcgcacgac gtaatcgact ttgccgccac
     1561 cgtatttctt ctcaatctgc tcgtcgagca ggaactgcga ttccagctcg ttcacaaacg
     1621 catagcccat cagctcgctg atttcttccg cactcaccgc gagatcgggc cgctccggcg
     1681 gctcgcccac gtcgagatat tcccggaacg cctgcagcgc gtttttcatg cccttgaggg
     1741 cggaagaggt gagcatgcgc gaataactca cgcgcgcgac gccgatcgcc tgcagccgct
     1801 tcaccgaaac aagcggcgtc gtaacgcgcc cgcgcagccc gagtcccatg ctgatgctga
     1861 ccggcgcctc gaccgcgtcg acgaagcgcc gaatctggtc ctccgagccg atcgcatcgg
     1921 ggtacaccat gtcggcaccc gctgcgagat agaccttcgc gcgctcgatc gcgctttctg
     1981 gcccctcgac cgcgatcgcg tcagtgcgcg cgttgatgat gaagtccggg tcgcgccggg
     2041 ccttgatcgc actctcgatc ttcttcgcca tttctgccgc gctgatgagt tccttgccgc
     2101 gcatgtgccc gcagcgcttg gggctcacct gatcctcgat gttgataccc accacccccg
     2161 cttcctcgaa atagcgcacg gtgtggtaga ccgtgaccgc attgccgtag ccggtgtcgg
     2221 cgtccgacat gacggggatc gagacgctgc gcgcgatgtg gtagcacgct tccacgttgt
     2281 cccgcagcgt catgacgccg atgtccggct ggccgaggcg tgaattggag agcccggccc
     2341 cggtggtgga ggcggcctgg aagcccatgg cttcgacgag acgcacgctg tagccgtcga
     2401 agactccggg cgataccacg atcttcccgg atgcaagcat ttcgcgcatg cggcgcgctt
     2461 tggtttccga tacgggctgg gctttctttt ccgtagggtt cattcgagtc ctctcaacga
     2521 agtcgttcaa tgctgcctaa attcgtcttc ccggcgtacg ccgggatcca gaatatgcct
     2581 tctggattcc ggctttcgcg ggaatgacga tacggtcgcc ggaatgacga ggcgggctgg
     2641 cgagctacgc ggtagccgcg cgcatgccgc gcggcgcgag cgccgcatcg ttgcacgcga
     2701 gctcagtgat cttccgcaca tcgtcgaggg cctcgaagcg ttccaccgcc gcgtgaattc
     2761 ctgcgacggt gtcccgttcc agcacgcgcg cggcgcagtt ctc
//
LOCUS       DASURG010000353         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_367731, whole genome shotgun sequence.
ACCESSION   DASURG010000353 DASURG010000000
VERSION     DASURG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367731"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFY80_06200"
     CDS             <1..753
                     /locus_tag="VFY80_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX6007534.1"
                     /translation="DEVALFEAAYARRMPMMLKGPTGCGKTRFLEFMAFELKRPLVTV
                     SCHEDLTSSDLVGRFLLEGAETVWQDGPLTRAVKVGAICYLDEIVEARTDSTVVIHSL
                     TDHRRKLTIEKKGMEIEAHEDFMLVISYNPGYQTVLKDLKPSTKQRFVAINFDFPPEE
                     IERRIIVKEVPGIAPEIAHQLVAAGRKARLLKDHGLEEATSTRALVYAASMMSAGLDP
                     IAACQVAMVNPITDDMELAEAMMEIVQAHFAA"
     gene            768..>2257
                     /locus_tag="VFY80_06205"
     CDS             768..>2257
                     /locus_tag="VFY80_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007535.1"
                     /translation="MTDRTAAIQDLLHELRDASLVASREVEHVLPAIRLHGDEIALAW
                     LIAGRRLFDFDREAGRAFIRGSAAAEQISETVMPWTEQALQFVRWRGSWRALEGFMMN
                     LPRAYGSLGHAGERRWAEIGLTWCGRQLESGSAYFGMPVTDLSGRQGIAGIEQLAAPA
                     EELFESRKLLLSTYLAGAVRVRNLLGAQAILPWALRGADIMQSGRARGEAYFRLESEE
                     SLALLLEHLPGFRLSDRNRLLGMLLDVWFGDTFDLKESSWSPEKGRPFVETDGRSLFF
                     PAVMANRDEAVLGVLHGAGHMRYGTFDRLAMKEMFSTANVEFPESGPVSWVPLYMRYG
                     DDALRFQLIFDLCEDLRVDFRIQHAVPNYLSRLAAAARAAPPRPPEAAAYFDLALASI
                     EEAMTATHASAPRGKQFGPLFDPAATVADAYRIASAIYSNDELPRVGDLEAFHAAYLP
                     GRSPNATRLAHPQQRQEQQTQAGAEQQSEPEEQGEDEGETAQNAESG"
BASE COUNT          400 a          727 c          747 g          383 t
ORIGIN      
        1 gacgaggtcg cgctgttcga ggcggcctat gcccgccgca tgccgatgat gctgaagggt
       61 ccgaccggat gcggcaagac ccgctttctc gagttcatgg cgttcgaact gaagcgtccg
      121 ctcgtcacgg tttcctgcca cgaggatttg acgagctccg atcttgtcgg gcgctttctg
      181 ctcgaaggcg cggaaacggt ctggcaggac gggccgctta cgcgcgcggt gaaggtgggc
      241 gccatctgtt atctcgacga gatcgtggag gcgcgcaccg actcgaccgt ggtcattcat
      301 tcgctcaccg atcaccggcg caagctcacg atcgagaaaa agggcatgga gatcgaggcg
      361 cacgaggact tcatgctcgt gatctcctac aaccccggat accagacggt actgaaggac
      421 ttgaagccct ccaccaagca acgcttcgtc gccatcaatt tcgatttccc gcccgaggag
      481 atcgagcgca ggatcatcgt caaagaagtg ccgggcatcg cacccgaaat cgcgcaccag
      541 ctggtggcgg ctggacgcaa ggcgcggctc ctgaaggacc acgggctcga agaggcgacc
      601 tcgacgcgcg cgctcgtcta cgcagcgagc atgatgagcg ctggccttga cccgatcgcg
      661 gcgtgccagg tagcgatggt caatcccatc accgacgaca tggaactcgc cgaagcgatg
      721 atggaaatcg tgcaggcgca ctttgcggct tagatacgcc gcgcgagatg accgaccgta
      781 ctgccgcgat acaggatctg ttgcacgagc tccgggacgc gagtctcgtc gcgtcccgcg
      841 aagtcgagca cgtgctgccc gccatacgac tccacgggga tgaaattgcg ctcgcctggc
      901 tcatcgccgg ccggcggctg ttcgatttcg atcgggaagc gggccgtgcc tttatccggg
      961 gtagcgcagc cgcggagcag atctcggaaa cggtgatgcc gtggaccgag caggcgcttc
     1021 agttcgtgcg ctggcgcggc tcctggcgcg cgctcgaagg cttcatgatg aacctgccgc
     1081 gcgcgtatgg ctcgctcggg catgccggcg agcggcggtg ggcggaaatc gggctcacgt
     1141 ggtgcgggcg gcagctcgaa agcggcagcg catacttcgg catgccggtg accgatcttt
     1201 cggggcgcca gggcatcgca ggcatcgagc agctcgcggc gcctgccgaa gaactcttcg
     1261 aatcgcgcaa actgctgctt agcacctatc tcgccggtgc ggtgcgcgta cgcaatctcc
     1321 tcggcgcaca ggcaatcctg ccctgggcgc tgcgcggggc ggacatcatg cagtcgggtc
     1381 gcgcgcgcgg cgaagcgtat ttccgtcttg aatccgaaga gagcctggcg ctcctgctcg
     1441 agcatctgcc cggtttccgg ctttccgacc gcaaccggct gctcggcatg ctgctcgacg
     1501 tgtggttcgg agacaccttc gatctcaagg aaagcagctg gtcgccggag aaagggcggc
     1561 cgttcgtgga aaccgacggg cgcagtctct tctttccggc ggtgatggcg aaccgtgatg
     1621 aagcggtgct cggcgtgctc cacggcgctg gccacatgcg ctacggcacg ttcgaccggc
     1681 tcgcgatgaa ggaaatgttc agtaccgcga acgtcgagtt tccggaatcc ggtccggtat
     1741 cgtgggtgcc gctctacatg cgctatggcg acgatgcact gcgcttccag ttgatcttcg
     1801 atttgtgcga ggacctgcgc gtcgattttc gcatccagca tgccgtgccg aactacctct
     1861 cgcgtctcgc cgcggcggcg cgcgcggcgc cgccgcgccc accggaagcc gccgcgtact
     1921 tcgatctcgc gctcgccagc atcgaagaag cgatgaccgc tacgcacgcg agcgcgccgc
     1981 gcggaaagca gttcggcccg ctcttcgatc cggccgccac cgttgccgac gcgtatcgta
     2041 tcgccagcgc gatctacagc aacgacgagt tgccgcgcgt cggtgacctc gaagcgttcc
     2101 acgcggcgta tctcccgggc cgctcgccga acgccacgcg actcgcgcat ccccagcagc
     2161 gccaggagca gcagacgcag gccggcgccg agcagcagag cgagcccgaa gagcagggcg
     2221 aggacgaggg cgaaaccgcg cagaacgcgg agtccgg
//
LOCUS       DASURG010000354         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1287591, whole genome shotgun sequence.
ACCESSION   DASURG010000354 DASURG010000000
VERSION     DASURG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287591"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFY80_06210"
     CDS             complement(<1..389)
                     /locus_tag="VFY80_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6007536.1"
                     /translation="MKREPENRLITAMQEAVVRTPVIELDGVTKTYRNGDLAVEVLHG
                     IDLAIHPGEFVAIVGQSGSGKSTLMNILGCLDRPTTGTYRFLGEDVSRLGRDELARLR
                     REAFGFVFQSYNLIAGANATENVEIPA"
     gene            complement(386..1759)
                     /locus_tag="VFY80_06215"
     CDS             complement(386..1759)
                     /locus_tag="VFY80_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6007537.1"
                     /translation="MKNRETATTMDRTEEKAQSATRKPAEREGRSRGKRIVRAVAAVA
                     VVGVLSAGGYFGWNAWNGANDAAARYTTAVVQRGDIEDAVTATGVLQPRDYVDVGTQV
                     SGQLRLLHVGIGDRVEKGKLLAEIDPTVYKTRVDADRAQINTLSAQLSDREAQLALAD
                     QQHRRQLNLMKEQATTEEALQGAEAALKSAKAQINVLKAQIEQTRSSLRGNEANLGYT
                     RIYAPMAGTVVSISAKQGQTLNANQQAPIILRIADLSTMTVQSQVSEADVSRLKVDMD
                     VYFTTLGSQGKRWSAKLRQINPTPEVVNNVVLYNALFDVENRDGTLMTQMTAQIFFVT
                     AQAKDAVYVPVAALRPAGARSGATRRAANAESKGGGARSGERREQAAPDRSRFSGGRG
                     TVRVMNQDGAIEEREVQVGVVSRVSAQIVSGLEPGEKVVTGTAAPAAPGRARTATANA
                     PRMGPRI"
     gene            1879..>3143
                     /locus_tag="VFY80_06220"
     CDS             1879..>3143
                     /locus_tag="VFY80_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6007538.1"
                     /translation="MDKLRFDRLHVKLFAAIAGAVALITVAAYFVFTASFEYGFIEYL
                     KRADEVRLSRMVERLADGYRREGSWSWIAEDRERWIEMSRDVLGLTRAPADATLPGVS
                     RPPRRDTPLTIDPRLMLFDPARRVLVGRAELAPRAQLKPIEVGADTVGYLGYVPRPEV
                     VESIERVYVQRQRLAFGVIAVGMLAAALVLAAGIAYWLTRRIRSLALATDALIRGDYA
                     ARVEVRGNDELARLAADVNTLAHTLGAARESRQQWIADIAHELRTPLTVLRAEIESLE
                     DGVRPIDEASIRSLAGETQRLARLVEDLHTLSLSDLGALSYHREPLDVAELLGDILAG
                     QSGAIAAQSLELETHFKAGAMVLADAARLTQVFMNLLQNSLRYTDAPGRIIVSVRRTG
                     ASVVIQWEDTAPGVAAAALPRLTDRLFRV"
BASE COUNT          453 a         1036 c         1031 g          623 t
ORIGIN      
        1 atcgcgggaa tctccacgtt ttcggtcgcg ttcgcgcccg cgatcaggtt gtagctctgg
       61 aacacgaatc cgaacgcttc acgccgaagc cgcgccagct cgtcgcgccc gagccgcgag
      121 acgtcctcgc cgagaaagcg ataggtgccg gtggtcggac ggtcgaggca tcctagaatg
      181 ttcatgaggg tcgacttgcc cgatcccgac tgaccgacga tcgcgacgaa ttcgcccgga
      241 tggatggcga ggtctatgcc gtgcagcacc tcgacggcga gatccccgtt gcgataggtc
      301 ttggtgacgc cgtcgagttc gatcacgggc gtgcgcacca ccgcctcttg catggccgtg
      361 atcagacggt tttccggctc gcgcttcata tgcgaggccc catgcgcggt gcgttcgcgg
      421 ttgccgtgcg cgcgcggccg ggtgccgccg gtgcggcggt gcccgtcacc actttctcac
      481 ccggttcgag accggacacg atctgcgccg acacgcggct cacgacaccg acctgcactt
      541 cgcgctcttc gatcgccccg tcctggttca tgacacgcac cgttccccgg cccccggaga
      601 accggctgcg atccggtgcg gcctgctcgc gtcgctcgcc gcttctcgcg ccgccgccct
      661 tgctttcggc attggccgct cgtcgcgttg cgcccgaacg tgcgcctgcc gggcggagcg
      721 ctgcgaccgg cacatacacc gcgtccttcg cctgcgcggt cacgaagaag atctgcgccg
      781 tcatctgcgt catgagcgtg ccgtcgcggt tctcgacatc gaagagtgcg ttgtagagca
      841 cgacgttgtt gacgacctcc ggcgtggggt tgatctgcct cagcttcgcg ctccagcgct
      901 tgccctgact gccgagagtc gtgaagtaaa cgtccatgtc gaccttgagc cgcgacacgt
      961 ccgcttccga gacctgcgac tggaccgtca tcgtcgaaag atcggcgatg cgcaggatga
     1021 tcggagcctg ctggttggcg ttgagggtct gcccctgctt ggcgctgatc gacaccaccg
     1081 ttcccgccat cggggcgtaa attctggtgt agccgagatt cgcttcgttg ccgcgcaggc
     1141 tcgagcgtgt ctgctcgatc tgcgccttca ggacgttgat ctgtgctttg gccgacttga
     1201 gcgccgcttc cgcgccctgc agtgcctcct cggttgtcgc ctgctctttc atgaggttga
     1261 gctggcgccg gtgctgctgg tcggcgagcg cgagctgcgc ttcgcggtcg ctcaattgcg
     1321 cgctgagcgt attgatctgc gcgcgatccg catccacccg ggtcttgtat accgtggggt
     1381 cgatttcagc caggagcttc cctttctcca cgcgatcgcc aatcccgacg tgcagcagcc
     1441 ggagctggcc ggacacctgc gtgccgacat ccacgtagtc gcgcggctgc agcacgccgg
     1501 tggccgtgac cgcgtcctcg atatccccgc gctgcacgac cgccgtggta tagcgcgccg
     1561 cggcatcgtt ggcgccgttc caggcattcc agccgaaata gccgccagcg ctcagtaccc
     1621 cgaccactgc aaccgccgcg actgcgcgca cgatacgctt gccgcgggac ctgccttcac
     1681 gctcggccgg cttgcgggtc gcggattgcg ctttttcctc ggtcctgtcc atggtcgtcg
     1741 ctgtttcccg gtttttcatg gctcgcagtc tgcgtccgca aagcgcacgg aatgcggacg
     1801 aaataaggaa gttctgtaac ggctcgcgaa agaagtgcat tcataattcg tgcgaaattg
     1861 cggtttagcc tcgcgaccgt ggacaagctt cgcttcgacc ggctgcacgt caagcttttc
     1921 gccgccattg cgggcgcggt cgccctcatt acggtcgccg cctacttcgt gtttaccgcg
     1981 agcttcgagt acggcttcat cgagtacctg aagcgcgcgg acgaagtgcg gctttcgcgc
     2041 atggtggagc gacttgcgga cggataccgg cgcgagggaa gctggtcgtg gatcgcggaa
     2101 gaccgcgagc gctggatcga aatgtcgcgc gatgtgctgg ggctcacgcg cgctcccgcg
     2161 gacgctacac tccccggcgt atcgcgtccg ccgcggcggg acacgccgct cacgatcgat
     2221 ccgcgcctca tgctgttcga tccggcgcgg cgggtgctcg tcgggcgcgc cgagctagcg
     2281 ccgagggcgc agttgaagcc gatcgaggtt ggggcggaca ccgtgggata tctgggctac
     2341 gtgccgcgcc cggaagtggt cgagtcgatc gagcgggttt acgtccagcg ccaacggctc
     2401 gcttttggcg tgattgcggt cgggatgctg gcggcagcgc ttgttcttgc tgctgggatt
     2461 gcctactggc tgacgcgccg catccgatcg ctcgccctcg cgaccgatgc actgatccgc
     2521 ggcgactatg cggcgcgggt cgaagtgcgg ggaaacgacg agctcgcgcg gctcgcggca
     2581 gacgtcaata cgctcgcgca cacgctcggt gccgcgcggg agtcgcgcca gcaatggatc
     2641 gctgatattg cacatgagct gcgcacgccg cttaccgtcc tgcgcgcgga gatcgagtcg
     2701 ctcgaggacg gagtgcggcc gatcgatgag gcttctatca ggtcactcgc gggtgagacc
     2761 cagcggctcg cacgcctggt tgaggacctt catacccttt cgctctccga tctgggcgcg
     2821 ctcagttacc atcgtgagcc gctcgacgtg gccgagctgc tgggcgacat cctggccggc
     2881 cagagtggcg cgattgccgc gcagtccctc gagttggaga cgcatttcaa ggcgggcgcc
     2941 atggtccttg cggatgccgc gcgcttgacg caggtcttca tgaatctcct gcagaatagc
     3001 ctgcgctata ccgatgcgcc gggccgcatc atcgtcagcg tgcggcgcac gggtgcttcc
     3061 gtcgtgattc agtgggaaga tacggcaccg ggcgtcgcgg ctgcggcgct cccgcggttg
     3121 accgatcggc tcttccgggt gga
//
LOCUS       DASURG010000355         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1493504, whole genome shotgun sequence.
ACCESSION   DASURG010000355 DASURG010000000
VERSION     DASURG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493504"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFY80_06225"
     CDS             <1..603
                     /locus_tag="VFY80_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6007539.1"
                     /translation="LDKLATDAGYAKVNDFLADIGNGKIGPRQLASALRPETVFEPGA
                     EKALVTHRVRSTSKDSVLVVGVDKLLTLPARCCKPVPPDPIVGFVTRGRGVSVHRANC
                     ANVRRLDARRKVRAEWGQAQGVTFPVEIEIEALDRTGLLRDISDVLSRERINVTATHS
                     LSSKLTARMRFTIEVTDGPQLKRALAMIREVRGVLGAARR"
     gene            628..1461
                     /locus_tag="VFY80_06230"
     CDS             628..1461
                     /locus_tag="VFY80_06230"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007540.1"
                     /translation="MEFYESLSGSLQGAISQVSSRIAEYLPNILGAVGLILLGWLVAR
                     LLRGLAVKSMHVLEVLLNRLSRSPGARALPSGSVDVVGGILFWVVLLFFAAAATHVLG
                     LGAFSSWLQGVVTYLPTLMAGALIIIAGVLLAGLARDLTVAALSAFPEHQRALLGRIV
                     QGTVLVTAVAVGADQIGVRITFLVIVAAVVLSAIVGGIALAVSLGSRAYVANLIGAHH
                     LRQAFGMGQRVRIGELEGTILEITAVSVVLETDQGRVTVPAKVYNETPIVLMAPKNGD
                     G"
     gene            1454..>2298
                     /locus_tag="VFY80_06235"
     CDS             1454..>2298
                     /locus_tag="VFY80_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6007541.1"
                     /translation="MAEANGLSTAFAEAHPADAARVLETLPAEDTAAFIGALAARLAA
                     PILREMGAPHAARIALLLDDAHVAAVAQFMGPQAAARIVQQLPHERQLTLLARLPVTT
                     AIAIRLLVGYPRGTCGAYMDPWPLALAPHLTAVDALEQVRRFEGDIDDCILVSNGDRR
                     LTGVLSLGDLVRAGPHEPLSALMSPPSHTVPALASVAIVANHPGWEEFRVLPVVEREN
                     RLVGALHRRALMAALVTPAQRPEPNVASGVFAAYWQVVSALTEVAVGALPSVPPVSRR
                     KESHER"
BASE COUNT          353 a          737 c          785 g          423 t
ORIGIN      
        1 ctcgacaagc tcgcgacgga tgcgggctat gcgaaagtca acgattttct cgccgacatc
       61 ggcaatggaa agatcggtcc gaggcagctc gccagcgcac tgcgtcccga gaccgtattc
      121 gagcccggtg ctgaaaaggc actggttaca caccgtgtgc gatcgacctc aaaggacagc
      181 gtgctcgtgg tgggtgtcga caagctcctc accttgccgg cgcgctgctg caagcccgta
      241 ccgcccgatc cgatcgtcgg cttcgtgacc cggggacggg gcgtgagcgt gcaccgcgcg
      301 aactgcgcga acgtccggcg cctcgatgcc cgacgcaagg tgcgggccga gtggggccag
      361 gctcagggtg tcacgttccc agtcgaaatc gagatcgagg cgctcgaccg caccgggttg
      421 ctgcgagaca tttcggacgt gctgtcgaga gagcgcatta acgtgacggc cacgcattcg
      481 ttgagcagca aactcacggc gcgcatgcgc ttcacgatcg aagtgacgga cgggccgcag
      541 ttgaaacgcg cgctcgccat gatccgcgag gtgcgtggag tcttgggcgc ggcgcggcgc
      601 tgatgttcga gggtctgggg gaggcggatg gaattctatg aaagtctgag cggctcgctg
      661 caaggcgcca tatcgcaggt ctcgtcgcgc atcgcggagt acctgcccaa cattctgggt
      721 gccgtcgggc tcatcctcct cggatggctc gtcgcgcgcc tgctgcgcgg tctcgcggtg
      781 aagtcgatgc acgtcctcga agtgctgttg aatcggctct cgcgcagccc gggcgctcgc
      841 gcactgcctt ccggctcggt cgatgtcgtt ggggggattc tcttctgggt ggtgctgctg
      901 ttctttgccg ccgcggcaac gcacgtgctg ggactgggcg cgttctcttc gtggctgcag
      961 ggggtcgtga cctacctccc aacactcatg gcgggcgcgc tcatcattat cgcaggcgtg
     1021 ctgctcgcgg gccttgcgcg cgatctcacg gttgcggcgc tgtccgcatt tcctgaacat
     1081 cagcgtgcgc tgcttgggcg gatcgtgcag ggcaccgttc tcgtgacagc ggtggccgtt
     1141 ggcgcggacc agatcggcgt gcggatcacc tttctcgtca ttgtggcagc ggtggtgttg
     1201 agcgcgatag tgggcggcat cgcactcgcc gtgagcctgg gttcgcgcgc atacgtggcg
     1261 aacctgatcg gcgcgcacca tcttcgccag gctttcggaa tggggcagcg tgtccggatc
     1321 ggcgaactcg agggcacgat actggagatt accgccgttt cggtcgtgct cgagacagat
     1381 cagggccggg tgaccgtgcc cgccaaggtc tacaacgaaa caccgatcgt cctgatggcg
     1441 ccgaagaacg gcgatggctg aagcaaacgg tctctccacc gcattcgcgg aagcgcatcc
     1501 ggccgacgcc gcgcgcgtgc tggaaacact gccggccgag gataccgccg ccttcatcgg
     1561 tgcactcgcc gcgcggctcg ccgcaccgat cctgcgcgag atgggcgcgc cccatgccgc
     1621 gcgcattgcc ctgctgctcg atgacgcgca cgtggctgcc gtggcccagt tcatggggcc
     1681 acaggccgcg gcgcggatcg ttcagcagct tcctcacgag cgacaactta cgctgcttgc
     1741 gcgcctgccg gtcacgacgg cgatagcgat ccgcctactc gtcggttatc cgcgcggcac
     1801 gtgcggcgcg tatatggacc cgtggccgct cgcgctcgcg ccacatctca ccgcagtcga
     1861 tgcgctggag caggtacgac ggttcgaagg tgacatcgac gactgcattc tcgtttcaaa
     1921 tggcgatcgt cgcctgaccg gcgtgctcag cctgggcgac ctcgtgcgcg ccgggccgca
     1981 cgagccgctc tccgcgctga tgagcccgcc atcgcatacc gtgcctgcgc tcgcctcggt
     2041 ggccatcgtg gccaatcacc cggggtggga agagttccgc gtgctgccgg tagtcgagcg
     2101 ggagaaccgg ctggtgggtg cgctgcaccg ccgcgcgttg atggcggcgc tggtgacccc
     2161 ggcacagcgc ccggagccca atgtcgcgag tggggtgttc gccgcctact ggcaagtggt
     2221 ctcggcactg accgaagtcg cagtcggcgc gctgccttcc gtgccacctg tttccagacg
     2281 aaaggaaagt catgagcg
//
LOCUS       DASURG010000356         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_573223, whole genome shotgun sequence.
ACCESSION   DASURG010000356 DASURG010000000
VERSION     DASURG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573223"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..2062
                     /locus_tag="VFY80_06240"
     CDS             2..2062
                     /locus_tag="VFY80_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6007542.1"
                     /translation="MLCSCNRTMALDGQRLAQALGWPTAPRIDDELCRRHVASFEAAI
                     KSGDDVVVACTQEAPLFSELHSELSARGRITFVNIRETAGWSAQARQATPKVAALLAL
                     ASLPDPEPVPAVTYKSNGSVVIIGPAETALYWAERLADRLEVCVLFTDSGARAELPSE
                     RCYPVYSGSVKSVIGYLGNFELSWEQTNPIDLEVCTRCGACIEACPEHAIDYAYQIDL
                     DKCRSHRKCVAACGEIHAIDFARAARARTDRFDLVLDLSIEPTIRLHQPPQGYFAPGR
                     DPAEQALAVGRLAEMVGEFEKPKYFEYRQNVCAHSRSEIVGCRKCIDVCSTQAISSDV
                     EQNRVQVEPHLCMGCGGCATVCPSGAMRYAYPRMPDMGIRLRTVLQTYREAGGRDACL
                     LLHDTEGGRLVARLGRRRRGLPAHVIPLEVFHIGGIGPDLMLGALALGAAQVAILATG
                     TEAPEYREALRREMAWAESVLAALGYGSGRLRLIEAENLAAFEPALWGWERQEETRPA
                     SFNFSDDKRTTLDFALDHLLRHAASPQHELPLPAGAPYGRVSVNRDTCTLCMACVGAC
                     PESALLDSKEAPQLRFIERNCVQCGLCAKTCPEDAITLSPRLLFTADAKREVVLNEAE
                     AFACVKCGKPFGTKQMIESMVGRLGAHSMFAEAGALERLRMCADCRVVDLMHNSSQGS
                     IFEW"
     gene            2282..2770
                     /locus_tag="VFY80_06245"
     CDS             2282..2770
                     /locus_tag="VFY80_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX6007543.1"
                     /translation="MVDEEASQTAFGQAWHALQSAARAAAPEAVRNEYDSAFITAGRP
                     PVFLYGSVYQTGFLMDKPLARLRDDLAHIGLARRADVGEPEDHISALCDVMRFLITGA
                     DGVPPATLEAQREFFEHHIASWYARLCEAIETAEQTSFYRSVAAFAREFFDLEVRSFE
                     IA"
BASE COUNT          510 a          873 c          916 g          516 t
ORIGIN      
        1 actgttgtgc agttgcaatc gcaccatggc cctcgatggc cagcgcctgg cgcaagcgct
       61 gggatggccg accgcgcccc gcatcgacga tgaactgtgc cggcggcatg tggcgtcatt
      121 cgaggcggcg atcaaaagcg gggacgatgt cgtcgtcgcc tgcacgcagg aagcgccgct
      181 gttcagcgaa ttgcacagcg aactcagtgc acggggccgc atcacgttcg tcaatattcg
      241 ggagaccgct ggatggtccg cgcaagcgcg acaggcaacc cccaaagtcg cggcgttgct
      301 ggcgctcgcc agccttccgg atcccgaacc cgtccccgcc gtcacctaca agtcgaacgg
      361 aagcgttgtc atcatcgggc cggccgagac cgcgctctat tgggcggagc ggctggctga
      421 tcgccttgag gtctgcgtac tctttaccga ttccggagcg cgtgccgagc ttccgtccga
      481 gcgctgttac ccggtgtatt cggggagcgt caagtcggtc atcggctatc tgggcaattt
      541 cgagctctcc tgggagcaga caaacccgat cgatctcgaa gtctgcaccc ggtgcggcgc
      601 gtgcatcgag gcgtgccccg agcacgcgat tgattacgcc taccagatcg acctggacaa
      661 gtgccgttcc caccgcaagt gcgtcgcagc ctgtggcgaa atccacgcaa ttgacttcgc
      721 gcgcgccgcc cgggcgcgga ccgaccgctt cgatctcgtg ctcgatctct caatcgagcc
      781 gacgatccgg ctccaccagc cgccgcaggg ttatttcgcc ccgggacgcg atcccgccga
      841 gcaggcgctc gccgtcggcc gcctcgccga gatggtgggc gagttcgaga agcccaagta
      901 tttcgagtat cgacaaaacg tctgcgctca cagccgctcg gaaatcgtgg gctgccgcaa
      961 atgcatcgac gtgtgttcga cgcaggcgat ctcgagcgat gtcgagcaga accgcgtcca
     1021 ggtggagccg catctgtgca tgggctgcgg cggctgtgcg acggtgtgcc cctcgggcgc
     1081 gatgcgctat gcgtatccgc gcatgccgga tatgggaatc cggctgagaa cggtccttca
     1141 gacctatcgt gaggcgggag ggcgcgacgc gtgcctcctg ctgcacgata cggaaggcgg
     1201 gcggcttgta gcccgactcg ggcggcgccg caggggattg ccggcacacg tcattccgct
     1261 ggaggtattc cacatcgggg ggatcgggcc ggatctcatg ctcggggcac tggcactcgg
     1321 tgccgcccag gtcgccattc tggctaccgg aaccgaggcg cccgaatatc gggaagcgct
     1381 gcgccgggaa atggcatggg cggagagcgt gctcgcagcg ctgggctacg gcagcggtcg
     1441 cctgcgattg attgaggccg agaacttggc tgcgttcgag cctgcgctat ggggctggga
     1501 gcgccaggag gaaaccaggc ctgcgagctt caatttctcc gacgacaagc gcacgacgct
     1561 cgattttgcg ctcgatcatc tgctgcggca tgctgcatcg ccacagcacg agttgccctt
     1621 gcccgcgggc gcgccatacg gacgggtcag cgtcaacagg gacacctgca cgttgtgcat
     1681 ggcctgcgta ggtgcgtgtc ctgaaagcgc actgctcgac tccaaagaag cgccacagct
     1741 tcgcttcatc gagcgcaatt gcgtccaatg cggactctgc gcgaagacct gccccgagga
     1801 tgcgatcacg ctcagccctc ggctcctctt cacggccgac gcgaaacgcg aagtcgtcct
     1861 gaacgaagcc gaggcgttcg cctgcgtaaa atgcggaaag cccttcggaa ccaagcagat
     1921 gatcgagagc atggtgggcc ggctcggcgc ccattccatg tttgccgagg cgggcgcgct
     1981 cgagcggctc aggatgtgcg cggactgccg ggtggtggat ctcatgcaca attccagcca
     2041 aggctccatt ttcgagtggt gaaatccgga tgggccatac gaaatcgctt gaaccgccca
     2101 ttacttacag actaatattt ggtcacgaaa aatggtttcg gcggctgtag acagcagcac
     2161 cgcaccccag gaggaggtcg ctgcagaaga gcgtgcacgc gcggatgttt acgcattgct
     2221 cgcggcgctt ttctattccc ccccttctga cgatctgctg aagcgcatcg ccgccgccca
     2281 tatggtggac gaagaggcga gtcaaacggc gttcgggcaa gcgtggcatg cgctgcagtc
     2341 cgctgcgcgc gcggctgcgc cggaagcggt tcgcaatgaa tatgacagcg cgttcatcac
     2401 tgcgggacgg ccacccgtgt ttctgtacgg gtccgtgtac cagacgggat ttctgatgga
     2461 caagccgctc gcgcggctgc gggacgatct cgcgcacatt gggctcgcgc ggagggcgga
     2521 tgtcggcgag ccggaggatc acatctccgc cctgtgcgac gtcatgcgct tcttgattac
     2581 gggcgcggac ggcgtgccgc ccgcgacgct cgaggcccag cgcgaattct tcgagcatca
     2641 catcgcttcg tggtatgcgc gcctttgcga ggccattgag acggcggagc agacgtcctt
     2701 ctaccggagc gttgcggcct tcgcccgaga atttttcgat cttgaagttc gttcttttga
     2761 aatcgcttga cacaggagga acggcaatga cgaccagcaa actgaatcgg agaaa
//
LOCUS       DASURG010000357         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1288230, whole genome shotgun sequence.
ACCESSION   DASURG010000357 DASURG010000000
VERSION     DASURG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288230"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1121)
                     /locus_tag="VFY80_06250"
     CDS             complement(138..1121)
                     /locus_tag="VFY80_06250"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6007544.1"
                     /translation="MLRMTLAAGHYDRTEALRDGRVRAEGIDLTYIMLRVEEIFWRMA
                     QHREFDASEFSLGGYVVRRGRGADDLIAIPVFPSRFFRQSCIFVHDGSGIRSPQDLRG
                     KRIGVPEYQMTAAVWARGILADDYGVRATDVEWVQGGLEEPGRIPFEPAEPPGVTVSF
                     APADRSLAQMLESGEIDALVTARTPSTFRRDRRAVRRLFEHPFEVERDYFRRTGIFPI
                     MHTLVVKREILDANPWVAFTLFKAFSEAKELAMADLTQTSALPLSLPFLVEHAYQTMD
                     LMGEDFWPYGLDSNRHTLTTFVRYMHEQGLIPEPMTVETLFPESTQRTFRV"
     gene            complement(1430..2173)
                     /locus_tag="VFY80_06255"
     CDS             complement(1430..2173)
                     /locus_tag="VFY80_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6007545.1"
                     /translation="MATEQRRTNRISEEERELRIQLAAAYRLADKYGMSELIYTHISL
                     RVPGPTPTYLLNPYGMLFNQITASSLVKIDFAGNVAGPSEWPVNRAGVAIHGSILAAR
                     PDINCVFHTHTPYTTAVSALECGLLPMSQAALRFQGQTAYHEYGRAATDPAECTRLVK
                     DLGDKPIMILRNHGGITTGKTVCEAFIASFYLEKACQFQILAQSAGAPLAMPADEIIA
                     ASGGRPAGDERAWSSLLRMLDREDSSYRD"
     gene            complement(2521..>3225)
                     /locus_tag="VFY80_06260"
     CDS             complement(2521..>3225)
                     /locus_tag="VFY80_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6007546.1"
                     /translation="GVPAVSWWVLFLFVLSGLIQPILRFLTYAGIHYVGAAAGTTLRG
                     AHPLFSTFLAILFLGESLNPPIVLGTISIVAGVSLISWQGNQRQANFRSWHLAYPLSA
                     AFLAGVSHPLRRYSLNLANEPLYLAAVIGIVALPWLASATLLPGQKQKPVWDRRAMGP
                     FLIAGTFETLGILLVIVALSVGHVVVVSPIVATSPLWIVFGSWLFLRDIERLTLRTIL
                     GAICVVAGTIAISFVR"
BASE COUNT          671 a          980 c          972 g          602 t
ORIGIN      
        1 gactgtgcta cgaggaacaa gattcgagtt ctgacccgcc aaccgccgtt caaggttcaa
       61 agtatgaaac ggaggtcgct gcaggttgcc caacttgcaa gcgacgatgg taaggcgaga
      121 ttctatcagc ctccgcgtca caccctgaag gtccgctgcg tgctctccgg gaacagggtt
      181 tcaactgtca tcggctctgg gatcagtcct tgctcgtgca tgtagcgcac gaatgtcgtg
      241 agcgtgtgcc gattgctgtc gagcccgtat ggccagaagt cctcgcccat gaggtccatg
      301 gtctggtagg cgtgctcgac gaggaacgga agactgagcg gcagcgcgct tgtctgggtc
      361 aggtcggcca tcgccagctc cttggcttcc gagaacgcct tgaacagtgt aaacgcgacc
      421 caggggttgg cgtcgaggat ctctctcttc accaccaggg tgtgcatgat ggggaaaatg
      481 ccggttcggc ggaaataatc gcgctcgacc tcgaacgggt gctcgaagag ccgccgcacc
      541 gccctgcgat cccgccgaaa cgtcgaaggc gtcctggccg taaccagcgc gtcgatctct
      601 ccactctcga gcatttgcgc cagcgagcgg tccgcgggcg cgaaactcac cgtgactccc
      661 ggcggctcgg caggctcgaa tggaatgcgg ccgggctcct cgagcccgcc ttgcacccat
      721 tcgacgtcgg tggcgcgcac cccgtagtcg tctgccagga tgccgcgcgc ccacaccgcg
      781 gccgtcatct ggtactcggg tacgccgatc ctcttgccgc gaagatcctg cgggctgcgg
      841 atgccggagc catcgtggac gaagatgcag gactgccgga agaagcgcga cggaaaaacc
      901 gggatcgcga tgaggtcgtc ggcgccccgc ccgcggcgca cgacataccc gcccagcgag
      961 aactccgagg cgtcgaactc ccggtgctga gccatgcgcc agaagatctc ctccactcgc
     1021 agcatgatgt aggtcaagtc gatgccctct gcgcgcaccc gaccgtcgcg gagagcctcg
     1081 gtccgatcat agtgtcccgc agccaacgtc atgcgcagca tgcccacctc atccagaaga
     1141 atattatccc gcaaagcatg tcctgggcgt cgtcgaagga ccgcaaaggc gcgaagataa
     1201 tatgcctcgc gccaagaacg ccaaggacgc caagaacaaa atgatttcga agttcgaaat
     1261 ccgaaattcg aaacaaattg aaatttccaa aaaccggaaa ctccaaaaca agctcacgac
     1321 gtttgtattc ggatttgatt gatgctttgg gctatgtctg tttcggactt cgatattcga
     1381 atttcgattt tctgatcttt gtgcaacaca tccgctattt cgttccggct cagtcccgat
     1441 aggaagagtc ctcgcggtcc agcatgcgca gcaggctgga ccaggcgcgc tcatcgccgg
     1501 cggggcggcc gccggacgcg gcgatgatct cgtccgcggg catggccaac ggcgcacccg
     1561 ccgactgcgc caggatctga aactgacacg ccttctcgag ataaaaggag gcgatgaacg
     1621 cttcgcacac ggttttgccc gtcgtaatcc cgccgtggtt gcggaggatc ataatcggct
     1681 tgtcgcccag gtccttgacg aggcgcgtgc attccgccgg atcggtggcc gcgcggccat
     1741 actcgtggta tgcggtctgc ccctggaacc ggagggcggc ctgcgacatc ggcagcagcc
     1801 cgcactcgag ggctgagacg gccgtcgtgt acggcgtgtg ggtgtggaac acgcagttga
     1861 tgtccgggcg cgccgctagg atcgacccat ggatcgcgac gccggcgcga ttcaccggcc
     1921 actcgctcgg tcccgcgacg ttgcctgcga agtcgatctt caccaggctt gaagcggtga
     1981 tctgattgaa gagcatgcca taggggttga gcagatacgt tggtgtcggt cccggaacgc
     2041 gcagagagat gtgggtgtag atcagctcgg acatgccgta cttgtcggcc agacggtacg
     2101 cggcggccaa ctggatgcgc aactcgcgct cttcctcaga tatccgattc gtgcgtcgct
     2161 gctcggttgc catcgtcacg atattggtac tccttctcgc tcggcaatct gcttgtgggt
     2221 cggtacaagt tcacgcggac agagccacgg tgctgcaagc gcccatcggt acaggaaatt
     2281 cggtacagga aattttgaac actctctttt ttcgctgacg cgagctatag cgccgcaccg
     2341 gaaaggtgtc aactctattg gaagattgta tgatccgaca cttccttaca actctcttgc
     2401 tttttgtctc gttcatcccg cgcgcgcatt tctcaattga agcccataag ctccttcagg
     2461 cgatcaccgc gccggcgttg cgcaggtccc gcgccttggc gtcattccgc ggcgcaagca
     2521 tcaccgaaca aatgaaatcg cgattgtgcc cgccacgacg cagatagcgc cgagaatggt
     2581 gcgcagggtc aaccgttcga tgtcgcgcag aaacagccag ctgccgaata caatccacaa
     2641 agggctggtg gcgacgatcg gtgagacgac cacgacgtgg ccgacgctca gggcgacgat
     2701 caccagcagg atgccgagtg tttcgaacgt tcccgctatg agaaacggcc ccatggcgcg
     2761 gcgatcccac accggttttt gcttttggcc cggcaatagc gtggcgctgg ccagccacgg
     2821 cagcgccacg atgccgataa ccgccgcaag gtagagcggc tcgttcgcaa gattgagcga
     2881 gtagcgccgc agggggtggg agaccccggc aagaaacgcg gcgcttagtg gataggcgag
     2941 atgccaggag cgaaagttgg cctgcctttg attcccttgc cacgagatca gcgaaacccc
     3001 cgccacaatc gaaatcgtgc cgagcacgat cggcggattc aacgattcgc ccaggaacag
     3061 aattgccagg aacgtgctga acaacggatg tgcaccacgg agggttgttc ccgccgcggc
     3121 cccgacgtaa tgaatgcccg cataggtgag aaaacgcagg atcggctgga tcagtccgct
     3181 cagtacgaaa agaaaaagca cccaccagga caccgccggc acacc
//
LOCUS       DASURG010000358         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1390942, whole genome shotgun sequence.
ACCESSION   DASURG010000358 DASURG010000000
VERSION     DASURG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390942"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..749)
                     /locus_tag="VFY80_06265"
     CDS             complement(126..749)
                     /locus_tag="VFY80_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007547.1"
                     /translation="MDALKVACVLRSGGDFTAEHVALLRDQVARFRPGVRFVCFSDVA
                     VPCERIALRHRGWRGFWAKLELFRELTGPTLYFDLDSLLLADPAPLIGAGEFVMIRHW
                     IHADRFASGVMYWDGDFSHVYRAFSPRLIRRYMQTMKRYGDQGFIQDVLGERITAFPE
                     GTVVSFKRQCKAGVPDGAVVVAFHGRPRPWNAAEKWVRSHYEHASAA"
     gene            complement(737..1357)
                     /locus_tag="VFY80_06270"
     CDS             complement(737..1357)
                     /locus_tag="VFY80_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX6007548.1"
                     /translation="MITAGQMRIPDGDAYFQRVFARTGDRFQAELFQRALRYTPGRKI
                     AIDIGAHVGSLTRQMAVHFASVIAFEPNPLNFACLQENATASTVAHFNVALGADEGYC
                     DMAQHADNSGCWRVIPGGAVRIAPLDSFAFENVDLIKIDVEGFEGQVIRGAQQTIAAC
                     DPTIVFEDNGLGPKLYSSAWVDPRKLLAELGYRRRERFEKDEIWTR"
     gene            complement(1354..1875)
                     /locus_tag="VFY80_06275"
     CDS             complement(1354..1875)
                     /locus_tag="VFY80_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007549.1"
                     /translation="MAGAKLLRPDAHLMLINEAWGAFEHAHHFLAGHGEKVHEFLAYR
                     TRQFPNAPPPTSLHASRRGGTDPAGVTHIWENVSTGGTSAWKAVRIGKAMGYEELILC
                     GCPINDSGYFFGKQRTGITVACDRIGDGEGRMYHNYRTTFAVRAKGEGQGVFSMSGYS
                     RELLGAPAARSAA"
     gene            complement(1978..2178)
                     /locus_tag="VFY80_06280"
     CDS             complement(1978..2178)
                     /locus_tag="VFY80_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007550.1"
                     /translation="MAIRRFEVSSVDKKGRTAVVRSTPGGTGSVVLQVNIEATLATDK
                     HAVIALLEQIAKRIAGERWPMR"
BASE COUNT          440 a          735 c          683 g          426 t
ORIGIN      
        1 ggcgtgcgac gtgcggggcg atgcgatggc ggaccgtgtc gagcacgtcg ctatcgcgcg
       61 tgccgtagtc tggatccgct gcccagatgg caccgtacag cgcagcctgg cgcgccgcgt
      121 ctacgtcaag cggctgaggc atgttcatag tgcgacctca cccatttttc cgcggcgttc
      181 cacgggcgcg gccggccatg gaacgcgaca acaaccgcgc cgtcaggcac accggctttg
      241 cactgccgct tgaagctgac gacggtgcct tcagggaatg cagtaatgcg ctcacccagt
      301 acgtcctgga tgaagccctg atcgccgtag cgtttcatcg tctgcatgta tcgccgaatc
      361 aaccggggcg aaaaggctcg atacacgtga gagaaatcgc cgtcccagta catcactccg
      421 gaggcgaagc gatcggcatg gatccagtgg cggatcatca cgaactcacc ggcgccaata
      481 agcggcgccg gatctgccag cagtagcgag tcgagatcga aatacagcgt tgggccggtg
      541 agttctcgaa agagctcgag cttcgcccag aatccgcgcc aaccacgatg ccggagcgcg
      601 atgcgctcgc atggcacggc aacgtccgag aagcatacga agcgcacgcc cggtctgaac
      661 cgcgcgacct gatcgcgtag caatgcaacg tgttccgcgg tgaagtcacc gcccgatcga
      721 agaacgcatg cgaccttcag cgcgtccata tctcgtcctt ctcgaagcgc tcgcgacggc
      781 gatagccgag ctcagcaagc agttttcgag gatccaccca tgcgctgctg tacagcttgg
      841 gaccgagtcc gttgtcttcg aagacgatcg tgggatcaca cgcggcgatt gtctgctgcg
      901 cgcctcggat gacttggcct tcgaagccct caacatcgat tttgatgagg tcgacgttct
      961 cgaaggcaaa cgagtccagc ggcgcgatgc ggaccgctcc acctgggatg acgcgccagc
     1021 atccggagtt gtcggcgtgc tgcgccatgt cgcaatagcc ttcgtcggcg ccgagcgcca
     1081 cattgaagtg cgcgacggtt gaggcggtgg cgttctcctg aaggcaggca aagttcagcg
     1141 gattgggctc gaacgcgatc acgctcgcga agtgcacggc catctgccgc gtcaggctac
     1201 cgacatgcgc cccgatgtcg attgcgatct tgcgcccagg ggtataccgc agcgcgcgct
     1261 ggaagagctc cgcctgaaac ctatctccgg ttcgtgcaaa aacgcgctgg aaataagcgt
     1321 ccccatccgg aatccgcatc tgtccggcgg tgatcatgcg gcggacctcg ccgccggcgc
     1381 gccgagcagc tctcgcgagt aacctgacat cgaaaagacg ccctgccctt cccccttcgc
     1441 gcgcaccgcg aatgtggtgc ggtagttgtg gtacatccgg ccttcaccat cgccgatccg
     1501 atcgcatgcg acggtgatcc cggtgcgttg cttaccgaaa aaatagccgc tgtcgttgat
     1561 cgggcaaccg cacagaatca attcctcgta tcccatcgcc ttaccaatcc gcaccgcttt
     1621 ccatgccgag gtgccaccag tcgacacgtt ctcccaaatg tgggtgactc ccgcggggtc
     1681 agtccctcct cttcggctgg cgtgcaatga ggttggcggc ggggcgttcg ggaattgcct
     1741 cgtgcgatac gccaagaact catgaacctt ttcgccgtgc ccagccagga aatgatgagc
     1801 gtgttcgaac gcgccccagg cttcattgat cagcatcaga tgcgcatcgg gcctcagaag
     1861 cttggcgcca gccacgtctt cgaaaacgca cgcagccgac cccacgatga gcagcgttcc
     1921 gctcatgcgg tctgtccact ctggaattga tcgcggagtc gccaagcgag cctccaacta
     1981 ccgcatcggc cagcgctcgc cagcaatcct tttcgcaatc tgctcgagca gtgcgattac
     2041 tgcgtgcttg tccgttgcca gcgtcgcttc gatattgacc tgcaggacga cgctgcccgt
     2101 accgccgggg gtactgcgca cgacggcggt gcgccccttc ttgtcgaccg aggagacttc
     2161 gaaccgtcga atagccataa cgcacccttg tgatggtggc caggcccgct aaggcctggc
     2221 caactgtgcc gaaggttagt ccgagtcgat cgcgccgaac gcgacggcgt tggtgatgtc
     2281 cacc
//
LOCUS       DASURG010000359         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_981991, whole genome shotgun sequence.
ACCESSION   DASURG010000359 DASURG010000000
VERSION     DASURG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981991"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /gene="ssb"
                     /locus_tag="VFY80_06285"
     CDS             complement(<1..251)
                     /gene="ssb"
                     /locus_tag="VFY80_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6007551.1"
                     /translation="MASVNKVILIGNLGADPETRYLPSGDAVTNIRIATTETWKDKNG
                     EKQEHTEWHRVAFFGRLAEIAGEYLKKGSPVYVEGRIQTR"
     gene            complement(251..1609)
                     /locus_tag="VFY80_06290"
     CDS             complement(251..1609)
                     /locus_tag="VFY80_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6007552.1"
                     /translation="MSAPELRASIGLSAIFGLRMLGMFAILPVFAIYAEHLPGGDDLT
                     LVGIAIGAYGFTQAILQIPCGWWSDRYGRRPVIYTGLAIFAAGSFIAAFGHHIYVVIL
                     GRVLQGAGAISAAVMALVADLTRDEHRTKAMAMIGSTVGATFALSLIAGPALDRVIGV
                     PGIFVMTGVLAIAAMGAAYAIVPKAAPRAAGTPAPADVACFGTVVKDPQLLRLNYGVL
                     ALHAALMALFIAVPFSLRDAGLALAQHWLVYLPALLGSFVLMLPPLLRSRQAGQIRKL
                     FIGSVALLLGAHLAMPWLMGSAVSIGLFLLLFFTPFNVLEACLPSLVSRIAPPQAKGT
                     AIGVFSSVQFFGIFLGAAVGGTLYGRWGIAGVVIFNAVLFVIWLMLARGMKVPATLST
                     RIYTLPEYAREGAESLAARLRMLPGVHEARIVAAEGRAYLKVDSAGFDEENVAKLFAG
                     ET"
     gene            1771..>2500
                     /locus_tag="VFY80_06295"
     CDS             1771..>2500
                     /locus_tag="VFY80_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6007553.1"
                     /translation="MIRIRGARTHNLKNVNLDLPRNRLIVITGLSGSGKSSLAFDTLY
                     AEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKASSHNPRSTVGTITEI
                     HDYLRLLFARVGDPHCPDHHITLTAQSVSQMVDHVLELPEDTRLMILAPLIVGRKGEQ
                     ADLFDELRAQGFVRVRIDGKVHEIDELPKLKKNVKHTVEVVVDRLRVRADAKQRLAES
                     FETALRHADGRAIAVEMDSGSEHLF"
BASE COUNT          493 a          841 c          777 g          389 t
ORIGIN      
        1 cgcgtctgta tgcgcccttc gacgtaaacg ggcgagcctt ttttcagata ttcgcccgcg
       61 atttcggcca gccggccgaa aaatgcgacg cggtgccact ccgtatgctc ctgcttctcg
      121 ccgttcttgt ccttccacgt ctcggtcgtg gcgatgcgga tattggtgac cgcatctccg
      181 ctcggcaggt agcgcgtctc gggatcggcg ccgaggttgc cgatcagtat cactttattg
      241 actgaggcca tcaggtttcc cccgcaaaca atttggctac attctcttcg tcaaaacccg
      301 cgctgtccac cttgagataa gcgcgcccct ccgccgccac gatccgcgcc tcatgcacgc
      361 ccggaagcat gcgcaaccgg gctgcgagcg actcggcacc ctcgcgcgca tattccggga
      421 gggtatagat gcgggtgctc aacgtcgccg gcaccttcat tccccgggca agcatgagcc
      481 agataacgaa cagcaccgcg ttgaagatga caacgcccgc gatgccccac cgcccataaa
      541 gcgttccccc caccgccgcg ccgagaaaaa tgccgaagaa ctgcacgctg ctgaagacgc
      601 cgatcgccgt gcccttcgcc tgcgggggcg cgatgcggga gacgagcgag ggcaggcacg
      661 cctccagcac gttgaacggc gtgaagaaga ggagcaggaa gagtccgatc gagaccgcgc
      721 ttcccataag ccatggcatg gcgagatgag cgccaagcag cagcgccacc gaaccgatga
      781 agagcttgcg gatctggccc gcctgcctcg agcgcagcag cggcggcagc atcagcacaa
      841 aagatcccag cagtgccgga agataaacga gccagtgctg cgccagcgcc aggcccgcgt
      901 cgcgcagcga gaacggtacg gcgatgaaga gcgccatcaa tgccgcgtgc agcgccagga
      961 cgccgtaatt caagcgcagc aactgcgggt ctttgaccac ggtgccgaag caggcgacat
     1021 ccgcaggcgc cggggtcccg gctgcgcgcg gggcggcttt cggcacaatg gcgtacgccg
     1081 cgcccattgc agcgatggcc agcacaccgg tcatcacgaa gatgcccggc acgccgatca
     1141 cgcggtccag cgccggaccc gcgatgagcg aaagcgcgaa cgtggcgccg acggtggatc
     1201 cgatcatcgc catcgccttg gtgcgatgct cgtcgcgcgt cagatcggcc acaagcgcca
     1261 tcaccgccgc cgaaatggcg cccgcgccct gcagcacccg cccgaggatg accacgtaga
     1321 tgtggtgccc gaatgcggcg atgaagcttc cggctgcgaa aatggcgagc ccggtgtaga
     1381 tgaccggccg tcttccatag cggtccgacc accagccgca ggggatctgc aggatcgcct
     1441 gcgtaaagcc ataggcgccg atcgcgatgc cgacgagcgt cagatcgtcg ccccccggca
     1501 gatgctcggc atagatcgca aagaccggaa gaatcgcgaa catgccgagc atgcgcagcc
     1561 cgaaaatggc ggagaggccg atgctcgcgc gcagttccgg ggcgctcatt cgctcgctgt
     1621 gggacatcga ttggagaaga ctggaacggg ttgggaatcg aacgcgaagt ccggtatatt
     1681 agcaggttta ccgcgcccac ccgcttgaaa gcaaacccgc aaccttccgt catcgcgctc
     1741 catccgccgg ggcccgcgcg cggcggcgag ctgattcgca tccgcggggc gcgcacgcac
     1801 aacctcaaga acgtcaatct cgatctgccg cgcaaccggc tgatcgtgat caccgggctg
     1861 tccggttccg gcaagtcgtc gctcgcgttc gatacgcttt acgccgaagg ccagcgccgc
     1921 tacgtcgaat cgctttccgc gtacgcccgc cagttcctgc agttgatgga gaagcccgac
     1981 gtcgacctga tcgaggggct ttcgcccgcc atctccatcg agcagaaggc ctccagccac
     2041 aatccgcgct cgaccgtcgg cacgatcacg gagattcacg actacctgcg gctgctcttc
     2101 gcgcgagtgg gcgacccgca ctgccccgac caccacatca cgctcacggc gcagagcgtg
     2161 tcgcaaatgg tcgatcacgt tctcgagctg cctgaagaca cgcggctcat gatcctcgcc
     2221 ccgctcatcg tcgggcgcaa gggcgagcag gccgaccttt tcgacgagct gcgcgcccag
     2281 ggcttcgttc gcgtgcgcat cgacggcaaa gtgcacgaga tcgacgagtt gccgaagctc
     2341 aagaagaacg tcaagcacac ggtggaagtg gtggtggacc ggttgcgcgt gcgcgccgac
     2401 gccaagcagc ggctcgcgga atcgttcgag acggcgttac gccacgcgga cgggcgcgcg
     2461 atcgccgtcg aaatggacag cggttcggag cacctcttct
//
LOCUS       DASURG010000360         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_777361, whole genome shotgun sequence.
ACCESSION   DASURG010000360 DASURG010000000
VERSION     DASURG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777361"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFY80_06300"
     CDS             complement(<1..238)
                     /locus_tag="VFY80_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007554.1"
                     /translation="MTRRRGGLAFLAIVWLCGAMGAAQAQSYPTKPIRIIVPASPGGG
                     TDAAARIIAAALTQSLGQQVVAENRPGAGGLIGTE"
     gene            complement(235..1317)
                     /locus_tag="VFY80_06305"
     CDS             complement(235..1317)
                     /locus_tag="VFY80_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007555.1"
                     /translation="MARTTELDVSSGDDAYADFFAFACRSQWTDGLPVIPPTPQRVER
                     MLQTMDGDPDEVMALMPPRQGKVTLRGIAANAVMAGCLPEYFPLVVAAVEAINDAGFP
                     LATLVGMRPETPFLLVNGPVRDEIALECGRGCLGPGWRANATIGRALRLIMINLGGLE
                     PGTYARACFASPLQFTFCAGEDEANSAWEPFHVERGFGRDQSVVSVFRVTSYIALFAP
                     LDWDQSPRGLLEHTGLSLGSVGNTAMYAGSLSCLLLFNHERIKLLADAGISKSEAKTL
                     LYEYAMLPAGIFRPSDLEALREKGRVVGGKVSLVERPEDIQIAVMGGPGIHTVYLPGF
                     AHESIPHMPVSRLIAARREPHKEETQ"
     gene            complement(1301..1468)
                     /locus_tag="VFY80_06310"
     CDS             complement(1301..1468)
                     /locus_tag="VFY80_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007556.1"
                     /translation="MLTSLGYPEVPVLVTPNPVVYLSEAQINERIDHLLRPIVASFDV
                     PQAGRADGQNH"
     gene            complement(1574..1897)
                     /locus_tag="VFY80_06315"
     CDS             complement(1574..1897)
                     /locus_tag="VFY80_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007557.1"
                     /translation="MTSARKSTLEVVVPVAEFMDVDRGVSGHASRRGIARKRVLLLPS
                     EKTSSIPFIDVLLGRMRSETPAAHVTTQRVDWAFFHPGRSAAIGAEIDRLASECDLMI
                     SGVAY"
BASE COUNT          390 a          710 c          658 g          351 t
ORIGIN      
        1 tttccgtacc gatcaatccg cctgcgccgg gtcgattttc ggcgaccacc tgctgcccaa
       61 ggctttgcgt aagcgctgca gcgatgatgc gcgccgccgc atcggttccg ccgcccgggg
      121 aagcaggcac gatgatccgg atcggcttcg tcgggtagct ctgcgcttgc gcagcaccca
      181 tggcgccgca aagccagacg attgcgagaa aggcgagccc gccgcgtctg cgcgtcattg
      241 cgtttcctcc ttgtgcggtt ccctgcgtgc cgcgatcaac ctgctgacgg gcatgtgcgg
      301 aatcgattcg tgcgcgaagc cgggcagata gaccgtatgg atgccgggcc cgcccatgac
      361 cgcgatctga atgtcctcgg gacgctccac cagcgaaacc ttgccgccga cgacgcgccc
      421 tttctcccgt aacgcctcga ggtcgctcgg cctgaagatg cccgccggga gcatcgcgta
      481 ttcgtagagc agcgtcttgg cctcgctctt cgaaatgccc gcatcggcga gcagcttgat
      541 gcgctcgtga ttgaaaagca gcaggcagga gaggctgccc gcgtacatcg ccgtattgcc
      601 gacggagccg agcgagagcc cggtatgctc gagcaggccg cgaggggact ggtcccaatc
      661 cagaggcgca aacagggcga tgtacgaggt gacgcggaat accgacacga cgctctggtc
      721 gcggccgaag ccgcgctcga cgtgaaacgg ctcccaagcg gagttggcct cgtcctcgcc
      781 cgcgcagaag gtgaactgca acggagacgc gaaacaggca cgcgcatagg tgccgggctc
      841 gaggccgccc aggttgatca tgatgagccg cagggcgcgt ccgatggtcg cattcgcccg
      901 ccagcccgga ccgaggcagc cgcggccgca ttcgagggcg atctcgtcac ggaccggacc
      961 gttcaccagc aggaaaggcg tctccgggcg catgccgacg agggtcgcga gcgggaagcc
     1021 ggcgtcattg atcgcctcca ccgccgccac caccagcggg aagtactccg gcaagcaccc
     1081 cgccatgact gcattcgcgg cgattccgcg caaggtgacc ttcccctgcc ggggcggcat
     1141 cagcgccatg acctcgtcgg gatcgccgtc catcgtctgc agcattcgct ctacgcgctg
     1201 cggcgtaggc ggaatgacgg gcagcccgtc ggtccactgg ctccgacacg cgaaggcgaa
     1261 gaaatccgcg taggcatcgt caccggacga tacgtcgagc tcagtggttc tggccatcgg
     1321 ccctcccggc ctgggggacg tcgaacgatg cgacgatcgg acgaagcagg tggtctatgc
     1381 gctcgttgat ctgcgcttcg ctcagataga cgacgggatt cggcgtaacg agcacgggca
     1441 cctcaggata gccgagcgag gtgagcaagc gccgggcgac cgcctcgaac gcatccgtaa
     1501 cgaccagcgt cgtcggtatt ccgcgcttct cgaactgggc acatgcgtgg caactccaca
     1561 gcgtgcagcc gcctcaatac gccacgcccg agatcatgag atcgcactcg ctcgcgaggc
     1621 ggtcgatttc agccccgatc gccgcactgc ggcccggatg gaagaatgcc caatcgaccc
     1681 tttgcgtcgt cacgtgcgcg gccggcgttt cgctgcgcat tcggccgagc aggacgtcga
     1741 tgaacggaat cgaggaggtc ttttcgctgg gcagcagaag aacccttttg cgcgcaatgc
     1801 ctcgacgcga cgcatgccct gaaactcccc ggtctacgtc catgaattcg gcgacgggaa
     1861 ccacgacctc gagcgtactc ttccgggcag aagtcatttc cggctctctc cttcggccag
     1921 cagcgctcca atggagctca tgctttgacc agtgacgtgc gtttgcaccg aacgttcgca
     1981 acccgaaaaa tccgaacgcc gtccacccac cgcgatccgg ctgcaaccat cctagctcgg
     2041 ctcgattcgc atctcgcgaa cgacccgaag ccatttctcg acctcgccgc gcatgaagcc
     2101 ttcgaattc
//
LOCUS       DASURG010000361         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1084093, whole genome shotgun sequence.
ACCESSION   DASURG010000361 DASURG010000000
VERSION     DASURG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084093"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..921)
                     /locus_tag="VFY80_06320"
     CDS             complement(16..921)
                     /locus_tag="VFY80_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007558.1"
                     /translation="MVEGKVVIVTGAGRGIGAAIARLMAREGAKVVVNDIGVSLSGEG
                     GDRTPAQEVVDDIRRAGGEAAANYDSVAEFASAAKIVQCALDHFGRIDCIVNNAGILR
                     DVIFHKMTEEDWDAVVTVMLKGAFNMCRNAADHFRRQESGSFIHMTSTSGLVGAMGQV
                     NYGAAKLGMVGLSKNIAIDMQRFNVRSNCIAPVAWTRMTASIPAKDPASAKRVEQRKQ
                     VTAEHNAPLAVFLASEAAKAVTGQVFSVRKNEIFLMSQSRPLRGIHRSEGWTPRTCAE
                     HMLPALKSWFFPLDRTADVFSWDSI"
     gene            complement(979..1890)
                     /locus_tag="VFY80_06325"
     CDS             complement(979..1890)
                     /locus_tag="VFY80_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX6007559.1"
                     /translation="MRRAADDIAGACNREATLPINYEKLIHWDIPEVEQQISRRDTML
                     YALGVGLGADPCDSEQLRFVYEKDLQALPTMAIILAYPGPWHAHPDTGITRSHVVHGE
                     QGFAIDRPLPVEGTVVGKTRVTGVVDKGQDKGALIMTSCEVRDKASGERICSLSSTTF
                     CRADGGFGGPSGPVRPVHPIPTSAPQKVCDLPTLPQAALIYRLSGDYNPLHADPRYAQ
                     KAGFKMPILHGRCTFGIAGHAILKACCGYDPARFRSMEGRFSSPVYPGETIRTEMWLD
                     GNIVTFRATVPARAVTVINNGRAQIVA"
     gene            1985..>2133
                     /locus_tag="VFY80_06330"
     CDS             1985..>2133
                     /locus_tag="VFY80_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX6007560.1"
                     /translation="MPFTLTSSAFAHHGEIPRRHTCQGEDVSVPLSWSGVPDGTKSLA
                     LIIDDP"
BASE COUNT          366 a          677 c          664 g          426 t
ORIGIN      
        1 gcgtccacga tgcggtcaga tcgagtccca gctgaataca tcggcggtgc gatcgagcgg
       61 gaagaaccag gacttgagtg ccggcaacat gtgctcggca caggtgcgcg gcgtccagcc
      121 ctcagagcga tggataccgc gcagcggccg cgactggctc atcaggaata tctcgttctt
      181 gcgcacggaa aacacctggc cggtgaccgc cttcgcggct tcactcgcca gaaacaccgc
      241 gagcggtgcg ttgtgctcgg cggtgacctg cttgcgctgc tcgacccgct tggcgctcgc
      301 cggatccttc gcggggatcg acgccgtcat gcgcgtccag gcgaccggtg cgatgcaatt
      361 ggaacggacg ttgaagcgtt gcatgtcgat cgcaatgttc tttgaaagcc ccaccatgcc
      421 gagcttcgcg gcgccgtagt tcacctgacc cattgcgccg acaagaccgg aggttgacgt
      481 catgtggatg aagctgccgc tttcctgcct gcggaaatga tccgcagcgt tgcggcacat
      541 gttgaaggca cctttcagca tgacggtgac gaccgcgtcc caatcctctt cggtcatctt
      601 gtgaaagatg acgtcgcgca ggatcccggc gttgttcacg atgcagtcga tccggccgaa
      661 atggtccagc gcgcactgga cgatttttgc tgccgaagcg aattcggcga cgctgtcgta
      721 gttcgccgcg gcctcgcctc ccgccctgcg gatatcgtcc accacctcct gcgcgggtgt
      781 gcgatcgccg ccttcgcccg acagagaaac gccgatgtcg ttgacgacga cttttgctcc
      841 ttcgcgcgcc atcagtcttg cgatcgccgc accgatgccg cgcccggcgc cggtgacgat
      901 gaccactttg ccttcgacca ttccctcttg attcactttg tcctccgaaa atccggccgt
      961 tggtgccgag cccatcggct acgcgacaat ttgtgcgcgc ccgttgttga tcaccgtgac
     1021 cgcgcgcgcc ggtaccgtcg ctctgaacgt cacgatattg ccgtcgagcc acatctcggt
     1081 gcgtatcgtc tcgccgggat aaacgggaga cgagaagcgg ccttccatgc tgcgaaaacg
     1141 cgcgggatca taaccgcaac aggccttcag gatcgcgtgc cccgcgatgc cgaaggtgca
     1201 gcgtccgtgc aggatcggca tcttgaagcc cgccttttgc gcgtacctgg ggtcggcgtg
     1261 aagcggattg tagtcgccgg agagccgata gatgagcgcg gcttgcggca gtgtgggcaa
     1321 atcgcagacc ttctgcggcg cgctcgtggg gataggatgg accggccgga ccgggcccga
     1381 gggaccgccg aagccgccgt ctgcacgaca gaacgtggtg gatgagagcg agcagatgcg
     1441 ctcgccgctt gccttgtcac gcacctcgca tgacgtcatg atgagcgcgc ctttatcctg
     1501 tcctttgtcc acgacgccgg tgacccgtgt cttccccacc acggtgcctt cgacgggcag
     1561 cggccggtcg atcgcgaaac cttgctcgcc atgcacgaca tggctgcgcg tgatgccggt
     1621 gtcgggatgc gcatgccagg gtccgggata ggccaggatg atcgccatcg tcggcagcgc
     1681 ctgcaggtct ttttcgtaaa cgaaccggag ttgctcggaa tcgcacgggt ccgcgcccaa
     1741 tcccacgccg agcgcgtaga gcatcgtatc gcgccggctg atctgctgct cgacttcggg
     1801 aatgtcccag tggatgagtt tttcgtaatt gatcggcaag gtcgcctccc gattgcaggc
     1861 gcctgcgatg tcgtccgccg ctcttcgcat gcggacggca agcccttcgg ggtagcctca
     1921 tgcttaacgc gcagaagcgc tgggctatac tagcacaggc cttttgcttt tccggaggtg
     1981 ggagatgccg ttcacgctca cttcatccgc attcgcgcat cacggcgaaa ttccgcggcg
     2041 ccatacttgt cagggcgaag atgtctcggt cccgctctcg tggagcgggg tgcccgacgg
     2101 aacgaaaagc ttggcgctca tcatcgatga ccc
//
LOCUS       DASURG010000362         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_63070, whole genome shotgun sequence.
ACCESSION   DASURG010000362 DASURG010000000
VERSION     DASURG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63070"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFY80_06335"
     CDS             complement(<1..635)
                     /locus_tag="VFY80_06335"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX6007561.1"
                     /translation="MTINDTPIFETDLKSLEFLHRGKVRDLYAVGEDKLLVVQTDRLS
                     AFDVILPTPIPGKGRVLTELSFFWFDKLRHIVPNHLTGIAPESVVAANERSQVTGRAM
                     VVRRLKPLLIEAVVRGYIIGSGWSDYQKTGAVCGIKLPAGLRPAEKLPEPIFTPASKA
                     PAGEHDENITYAEAEKAIGAKYAAQVRDLSVRLYSEAAEYAATRGIIIADT"
     gene            complement(636..1127)
                     /gene="purE"
                     /locus_tag="VFY80_06340"
     CDS             complement(636..1127)
                     /gene="purE"
                     /locus_tag="VFY80_06340"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX6007562.1"
                     /translation="MSTPLVGIVMGSQSDWDVMKHAARQLKDFGVPFEARALSAHRTP
                     DLLIEWIDKLVAAGAKCFIAGAGGAAHLAGVVASKTTLPVLAVPMPSKHLQGLDSLLS
                     MVQMPKGIPVATFAIGDAGAANAGLFAVSVLALEDKKLAAALAKFRAEQADAVKAVKL
                     PEV"
     gene            1273..>2171
                     /locus_tag="VFY80_06345"
     CDS             1273..>2171
                     /locus_tag="VFY80_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6007563.1"
                     /translation="MDITRTLARRLVESRYEDIPDAVRHEASRALLNWLGCAIGSAWH
                     ETVECALAAVRPFSGSAQAQLLGRPERLDILNAALLNGISSHVLDFDDTHARAIHPSA
                     PVLPALLALAEWKGLSGSRLVHAFVHGVEAEERIGLSVFPEHYDIGWHITGTAGVFGA
                     AAASGLLLGLNAQQMTWALGIAATQSAGLREMFGSMCKSLHPGRAAQNGLAAALLAAR
                     DFTSSEHAIEAPRGFAHVMSTKFDPAVIMESWGERYELMNNMYKPYACGLVVHAAIDG
                     CIQLQRAHGIRPEDVARIALKVSP"
BASE COUNT          355 a          737 c          683 g          396 t
ORIGIN      
        1 ttcgtgtcgg cgatgatgat gccgcgtgtg gccgcatact cggcggcctc gctgtaaagg
       61 cgaacggaga gatcgcgcac ctgcgccgcg tacttggccc cgatcgcctt ctcggcctct
      121 gcatacgtga tgttttcgtc atgctcgccg gcgggcgctt tcgaggccgg cgtgaaaatc
      181 ggctccggca gcttctccgc cgggcgcaat cctgcgggca gcttgatgcc gcacacggcg
      241 cccgtcttct ggtaatcgct ccagcccgag ccgatgatgt aaccccgcac cacggcttcg
      301 atgaggagcg gcttgagccg ccgcaccacc atcgcgcgcc cggtcacctg gctgcgttca
      361 ttcgccgcca ccaccgattc cggcgcgatg ccggtgagat gattcggaac gatgtggcgc
      421 aacttgtcga accagaagaa cgaaagctcc gtcagcaccc ggcccttgcc ggggatcggg
      481 gtcggcagga tcacgtcgaa ggccgacagc cggtcggtct gcaccaccag aagcttgtcc
      541 tcgcccacgg cgtagagatc ccgcaccttg ccgcggtgca ggaactcgag gctcttgaga
      601 tcggtttcga agatcggcgt atcgttgatc gtcatctata cctcgggcag tttgaccgct
      661 ttgacggcgt cggcctgctc cgcgcggaat ttcgcgagag ccgccgcaag cttcttgtct
      721 tcgagcgcca gcacggagac cgcgaacagt cccgcattcg ccgcacccgc gtccccgatc
      781 gcgaaggttg cgaccggtat gcccttgggc atctggacca tcgagagcag cgagtcgagg
      841 ccctgcagat gcttcgaagg catcggcacc gcgagcaccg gaagcgtcgt cttggaggcg
      901 acgacgccgg cgagatgcgc agcgccgccg gcgcccgcga tgaagcattt cgcccctgcc
      961 gcgacgagtt tatcgatcca ttcgatcaag agatcggggg tgcgatgcgc ggacaatgcc
     1021 cgcgcctcga acggtacacc gaaatccttg agctgccggg cagcgtgctt catcacgtcc
     1081 cagtcgctct ggctgcccat cacgatgcca accaacggcg tgctcatatc ctgtttgctc
     1141 cagcgttgcg cgagcgcgat attctagcga atgtttcgtt gtggcggcat cgatgctttg
     1201 ctagaatcct cccgcaatat aaggtgccgc gtgcttcgcg caccgttcgc cgtccgacgc
     1261 tccccgtctt gcatggacat cacccgcacc ctcgcccggc gtctcgtcga atcgcgctac
     1321 gaagacattc cggacgcggt gcgtcacgaa gcgtcccgcg cgctgctcaa ctggctggga
     1381 tgcgcgatcg gcagtgcttg gcacgaaacc gtcgaatgcg cgctggccgc agtgcgcccg
     1441 ttctccggat cggcgcaggc gcaactcctc gggcgtccgg agcggcttga catcctcaat
     1501 gccgcgctct tgaacggcat cagctcgcac gtgctggatt tcgacgatac gcacgctcgc
     1561 gcgatccatc ccagcgcgcc ggtcctgcct gcgctccttg cgctcgccga gtggaaaggc
     1621 ttgagcggtt cgcggctcgt ccacgctttc gtgcacgggg tggaagcgga ggagcgcatc
     1681 ggtctctcgg tctttccgga gcactacgac atcggctggc atatcaccgg cacggccggc
     1741 gtattcggcg ccgcggcggc gagcggcctt ctgcttggcc tgaacgcgca gcaaatgacc
     1801 tgggcgctcg gcatcgcggc cacccagtcc gcggggctgc gcgagatgtt cggcagcatg
     1861 tgcaagagcc tccatcccgg gcgcgcggcc cagaacggcc tcgccgcggc gctccttgcc
     1921 gcgcgcgatt tcacgagctc ggagcacgcg atcgaggcgc cgcgcggctt cgcgcatgtc
     1981 atgtccacga agttcgatcc cgcagtgatc atggaaagct ggggcgagcg ctacgagctc
     2041 atgaacaata tgtacaagcc ctacgcgtgc ggcctcgtcg tgcacgccgc gatcgatggc
     2101 tgcatccagc tccagcgcgc gcacggcatc cgtccggaag acgtagcccg cattgcgctc
     2161 aaggtgagtc c
//
LOCUS       DASURG010000363         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1391922, whole genome shotgun sequence.
ACCESSION   DASURG010000363 DASURG010000000
VERSION     DASURG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391922"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFY80_06350"
     CDS             <1..235
                     /locus_tag="VFY80_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX6007564.1"
                     /translation="KAYGSLVVTVSIKANGVLEKVEINRPSGWKILDEAALHIVRLAA
                     PFAPFPADIASDTDILSITRTWTFTRSDQLIAE"
     gene            251..1084
                     /gene="aroE"
                     /locus_tag="VFY80_06355"
     CDS             251..1084
                     /gene="aroE"
                     /locus_tag="VFY80_06355"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6007565.1"
                     /translation="MTDKYAVIGNPVAHSKSPFIHAEFARQTGQDIAYTAVLAPREGM
                     RAAVLAFRDAGGRGMNVTLPFKHEAWALATRREGFAEAAGAVNTLTFHGADIIGYNTD
                     GIGLVRDIKANLQCAIEGRRVLLMGAGGAAYGVVEPLMREHPDRLVIANRTPAKADAL
                     VAHFAGIAAWASRGVNARGYAELMNERFDLLVNATSAGLRDEMPPLPEESIAPDALAY
                     DMVYGRMTPFLHFARAHGARTADGTGMLVEQAAESFFIWRGVRPQTAPVIARLRSMGE
                     G"
     gene            1146..1844
                     /gene="mtgA"
                     /locus_tag="VFY80_06360"
     CDS             1146..1844
                     /gene="mtgA"
                     /locus_tag="VFY80_06360"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX6007566.1"
                     /translation="MLRFAWRWTWRLLLLALIALTLAQFWFAIHIWYWSRNNPEMTAF
                     MESRLQRMREKNPRAALKHQWVPYPRISVNLKRAIVAAEDAKFLDHGGFDWEAMQKAY
                     ERNLQRGKVVAGGSTISQQLAKNLFLSSDRSWWRKAQEAVITLMIETIMTKRRILEIY
                     LNVIEWGDGVFGAEAAARHHYGMGAASLNAAQAARLAAMVPSPRRYGPGQTTAYLARR
                     TEIILARMPAARVP"
     gene            1930..>2209
                     /locus_tag="VFY80_06365"
     CDS             1930..>2209
                     /locus_tag="VFY80_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6007567.1"
                     /translation="MQRRSFLTALGVLGVSGLARASQERMPLLAQAAPRTRTPDVIFV
                     PTPNDVVNKMLEMAKVTAKDVVYDLGCGDGRIVITAAQRYGCRGVGIDI"
BASE COUNT          402 a          706 c          731 g          370 t
ORIGIN      
        1 gaaagcgtac ggcagcctgg tggtgacggt ctcgatcaag gcgaatggcg tgctggaaaa
       61 ggtggaaatc aaccgaccct cgggatggaa gatactcgat gaggcggcgc tccatatcgt
      121 caggctggca gcaccgtttg cgccgtttcc cgccgatatc gcgagcgaca ccgatatcct
      181 gagcatcacc cgcacctgga ccttcacgcg ctcggaccag ctgattgccg aataggatga
      241 gcggaacgcc atgacggaca agtacgcagt catcggcaat ccggtagcgc acagcaaatc
      301 ccctttcatt cacgccgagt tcgcccgtca gacggggcag gacatcgcgt ataccgccgt
      361 gctggcgccg cgcgagggaa tgcgcgctgc cgtgctcgcc ttccgggacg cgggtggacg
      421 cggcatgaac gtgacactgc ccttcaagca cgaggcctgg gcgctcgcga cacgtcgcga
      481 aggctttgcg gaagcggcgg gtgcagtaaa cacgctgacg ttccatgggg cggacatcat
      541 cggctacaac accgacggca tcggcctcgt gcgcgatatc aaggcgaatc tccagtgcgc
      601 gatcgagggc cggcgcgtgc ttctgatggg cgcgggcggc gccgcgtacg gcgtggtcga
      661 gcctctgatg cgcgagcatc cggaccggct cgtcatcgcg aaccgcacgc cggcgaaggc
      721 cgacgcactg gtcgcgcatt tcgctgggat cgcggcatgg gcatcgcgtg gcgtgaacgc
      781 ccggggctac gccgaactca tgaacgagcg cttcgatctc ctcgtcaatg cgacgtcggc
      841 gggtctgcgc gatgaaatgc cgccgcttcc ggaggaaagc atcgcaccgg atgcgctcgc
      901 ctacgacatg gtgtacggca gaatgacacc cttcctgcac ttcgcccgcg cccatggcgc
      961 gcgcaccgcc gatggcaccg gcatgctcgt cgagcaggct gccgaatcgt tcttcatctg
     1021 gcgcggcgtg cgcccgcaga ccgctccggt gatcgcccgg ctgaggagca tgggtgaagg
     1081 ttgaccatca cccccccgga ggctgcaagc cgtacatgcc tgcttcgaag ctgcatgcct
     1141 gagcgatgct gaggttcgca tggcgctgga cgtggcggct cctgctgctg gcgctcatcg
     1201 cgctcacgct cgcgcagttc tggtttgcga tccacatctg gtactggagc cggaacaatc
     1261 ccgaaatgac cgccttcatg gaaagccgcc ttcagaggat gcgcgaaaag aatcccaggg
     1321 cggcgctcaa gcaccagtgg gttccctatc cgcgcatttc ggtgaatctg aagcgcgcca
     1381 tcgtcgccgc cgaggatgcg aagtttctgg atcacggcgg cttcgactgg gaggcgatgc
     1441 agaaggcata cgagcgcaac ctgcagcggg gaaaggtggt cgcgggcgga tcgaccatca
     1501 gtcagcagct cgcgaagaac ctctttctct cgagcgaccg ctcgtggtgg cgcaaggcgc
     1561 aggaggcggt gatcacgctg atgatcgaga cgatcatgac gaagcgccgc atcctcgaga
     1621 tctacctcaa tgtcatcgaa tggggcgacg gcgtcttcgg cgccgaggcg gctgcgcgcc
     1681 accactatgg gatgggcgcc gcgagcctca acgccgcaca ggccgcccgg cttgcggcga
     1741 tggtgccgag cccgcggcgc tacggccccg ggcagacgac cgcgtatctc gcgcgccgta
     1801 ccgagatcat ccttgcccgc atgccggcag cgcgggtgcc gtaagcgccg cgagcggctc
     1861 gccctttttc tgcaaagtta ggctaaagtt ccgctacgcc agccttggcg caacaaccgg
     1921 agaccatgta tgcaaagaag aagctttctg acggcgctgg gtgtcctggg cgtttcgggc
     1981 ctcgcgcggg cgtcccagga gcgcatgccg ttgctcgccc aggcggcgcc acgcacgcgc
     2041 acgccggacg tcattttcgt tcccacgccc aatgatgtcg tcaacaagat gctcgagatg
     2101 gccaaggtca cggcgaaaga cgttgtctac gacctggggt gcggcgacgg gcgcatcgtg
     2161 attaccgcgg cgcagcgcta tggctgccgc ggcgtcggca tcgatatcg
//
LOCUS       DASURG010000364         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_165692, whole genome shotgun sequence.
ACCESSION   DASURG010000364 DASURG010000000
VERSION     DASURG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165692"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFY80_06370"
     CDS             <1..462
                     /locus_tag="VFY80_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007568.1"
                     /translation="DTGVSKATVAKTRSARAANRNTVLVYPGTGGSVSVDGHTLKVPP
                     GAVAQPTFFMLQVVEANAIHVRLKAWRASDAAAVTQFPNVPVELSLDVSDLETLDLSE
                     LVVVYLRDGTYAGAKEPVPTVVSGSTRITGYLTHFSQYAVAFAREYSPGID"
     gene            476..2102
                     /locus_tag="VFY80_06375"
                     /pseudo
     CDS             476..2102
                     /locus_tag="VFY80_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(2103..>2405)
                     /locus_tag="VFY80_06380"
     CDS             complement(2103..>2405)
                     /locus_tag="VFY80_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6007569.1"
                     /translation="MIVCDGDVLAPEHISLRSRAHAETVAPSPAQPSVDDRDVIVIPK
                     TGLSLNDAERQLLESTLRLAKFNQSLAARMLGVSRPTIVRMMQKHGFRTRRDLVAD"
BASE COUNT          447 a          766 c          743 g          449 t
ORIGIN      
        1 gacaccggtg tgagtaaggc gacggttgcc aaaacgcgca gcgcccgggc ggccaatcgc
       61 aatacggtgc tggtatatcc cggaacgggg ggctcggtct cggtcgacgg ccatacgctg
      121 aaggtgccgc cgggcgcggt cgcgcagcct acgttcttca tgctgcaggt cgtggaagcc
      181 aatgccattc acgtcagact gaaagcctgg cgcgcgtccg acgcggcggc ggtcacgcag
      241 ttcccgaacg taccggtcga gctgtccctc gatgtgagcg acctcgagac gcttgacctc
      301 agtgagctgg tcgttgtata cctgcgcgat ggcacgtatg caggtgccaa ggagccggtg
      361 cccacggtcg tcagcggcag cacccggatc accggttatc tcacgcactt ttcgcaatac
      421 gcggtggcat ttgctcgcga gtattcgccg ggcatcgatt aaccaggagc tgagcatgcg
      481 cagaaggcgc gagccccgac ccggcgtgct gaatggctcg tcgggtcgtc acattttatt
      541 gactgtcctg ttcctcctcg gtttgggcgc cgtcaaggtg gaagcgcagc gtccgcggcc
      601 gggcgggaca gtcgtcattg ccggcggctc cgatctgcag agcctgaact cgatcgtcaa
      661 caccgatgca tggacgcgcg agttcatcga gcatgcgctc ttcttgcgcc tgcttacgct
      721 caaacccgat ctcagctacg cgccgcagct ggcccagtcc tatcgcctga tcggcgatac
      781 ggccgttatc ttcacgctcc ggcgagatgt acgttggcat gacggacgac gcacctccgc
      841 gcacgacgtc gcgttcacct tcgaacgcgt caagaacgaa gaaactgcgt cgccgcacgt
      901 cgaacagttc gccaactgga agagcgcgca ggtgctggat tccttccgca tccgtttctc
      961 cttcaagccg cacgtcgagc cgctctttgc ctggacgcag ctggccatca tgcccaggca
     1021 tctgctcgat tccataccgc ctgcgcgtct gcgccaggcc gcgttcaaca agcagccggt
     1081 gggcaacggt ccgttccggt tcgtttccca gcgcgccaac gaccggtggg tgttcgaggc
     1141 caaccggtcg tttccggctg cgctgggtgg caggccgtat ctgaaccgcg tcatctggag
     1201 ggtcatcccc gagaatacgg cgcaaatcac cgaaattcgg accggacagg tggatgtgat
     1261 tcggcccgcg cgcggagaac gtggagctgc tcgaccgcga gcccggcatg cgcgccatca
     1321 ttcgcccgtc caaccgctac aacatgatca cctggaacgg caagcgacca ggcctcgacg
     1381 atgcgcgcgt ccggcgcgcg ctcacgctgg cgctcaatcg tgcggaaatg ctgcagctac
     1441 tgcgacgcgg ttatggcacg attgcggtca gcccgatccc accctaccac tggtcgtatg
     1501 atcgctcact gcgcccgctc ccctttgata ccgtggcggc caagcgcctg ctgagtgagg
     1561 ccgggtgggt cgaccgcaac cgcgatggcg tgcgcgagaa tgccgcaggc cggccactcg
     1621 agatcgaact gcaggtggta gccaataacc agttcaaccg cgacctgggc gagttggtgc
     1681 gtgctcagct gcaacgcgtg ggtgtgaaga tcaacgtgcg gccgatcgac ttctccgtga
     1741 tgatccagaa catcaccaac ccggcgcgga attacgatgc cgcatatttg cagatgagta
     1801 ccgatctggt gctcaatttc cacgacgcat tccactcatc gacgctggat aacccgttcc
     1861 atgcgacggc gtatgcgaat ccggaagtcg atcgcatcct ggatgaggcg acgataacga
     1921 aagaccggca gcgcgccatt cagttgtggc gccggttcca gcgcatcatg cgcgatgacc
     1981 agcccatgac catgacctgg tggtcacccg atctgattgt cgtgcgggaa agactgaaag
     2041 gcgtgacgat ggatgtgcgc ggcgcgctgc tcacgctgcc gcgctggtat gtaacgaact
     2101 gatcagtccg ccaccagatc gcggcgcgtg cggaaaccgt gcttctgcat catccgcaca
     2161 atcgtgggtc gggatacgcc cagcatgcgt gcggctaacg actgattgaa tttcgccaga
     2221 cggagcgtcg actcgagcag ttgacgctcc gcgtcgttca ggctcaggcc cgtcttcgga
     2281 atcacgatca cgtcccgatc gtcgactgac ggctgcgccg gtgacggtgc gacagtctcc
     2341 gcgtgcgccc gtgaacgcag cgaaatgtgc tcgggcgcga gtacatcgcc atcacagacg
     2401 atcat
//
LOCUS       DASURG010000365         8181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1289583, whole genome shotgun sequence.
ACCESSION   DASURG010000365 DASURG010000000
VERSION     DASURG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289583"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFY80_06385"
     CDS             <1..584
                     /locus_tag="VFY80_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6007570.1"
                     /translation="AAPTEAASMGALGSILVTAVAGRMSFAVLRDTVYATTKITAMVM
                     FILICAQVFALAFRGLRGEDLIHDLFAFLPGGVNADIWFLMALIFVLGFFIEWIEISY
                     IAVPLFLPVFVQQNVDIVWLAMLICVNLQTSFLTPPFGWALFFLRGVAPPQVTTRHMY
                     LGVIPFIAMQIVGVVLIFFYPQIALWLPKVIGW"
     gene            655..2256
                     /locus_tag="VFY80_06390"
     CDS             655..2256
                     /locus_tag="VFY80_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX6007571.1"
                     /translation="MTMIHSIRGTRGMAVAPHSVAAQSALDILREGGNALEAAIGCAA
                     TIAVVYPHMNGIGGDSFWLMHVPGRKPRGIDACGRSGAAATRDWYAARGIADAIPCRG
                     GLAANTVAGTISGWDTAFALSRNWGGRLPLKRLLADAIYYAEHGMAVTRSQAVATETK
                     RAELESQPGFAATFLPGDKTPVEGMMFRQPRLAETLRRLSRGLTGFYRGRLARSMAHD
                     LAAAGSPLTVDDLSAHRAAVVAPLELRHSLATVYNLPPPTQGVISLLILGILDALHVG
                     KTGAASPEFIHLSVEATKRAFALRDRCVTDPEYGAIDAQGLLAPDRIREHASRVDARK
                     AAPWGGGRAPSDTVWLGVVDQAGRSVSVIQSVYHEFGSGIVLEESGVMWQNRGCSFSL
                     DPDALNTLQPGRKPFHTLNTALALFRDGRTLLYGTMGGDGQPQTQAAVFTRIAAFGLN
                     PQAAVSAPRWLLGRTWGQTSDTLKVEARFSGAILMALGKFGHEIEVLQDYDELMGHAG
                     AIVRNPSGVLEGGADPRSDGAVAAF"
     gene            complement(2312..2536)
                     /gene="rpmE"
                     /locus_tag="VFY80_06395"
     CDS             complement(2312..2536)
                     /gene="rpmE"
                     /locus_tag="VFY80_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX6007572.1"
                     /translation="MKPKIHPDYGEITVTCSCGNAWTTRSTLNRDLHVEVCSACHPFY
                     TGKQKIVDTAGRVEKFRQKYARKAAPKAAA"
     gene            complement(2602..3063)
                     /locus_tag="VFY80_06400"
     CDS             complement(2602..3063)
                     /locus_tag="VFY80_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007573.1"
                     /translation="MNSSAHVRPFIALLLLLAIGCASTPQASRERDAQAKQFHTHPAT
                     AAIYVFRPDNALDEESVLYVDERLVGATLPGTYFRIDVRPGKHRLHGIAGDTGSVELE
                     TRPGEIYFVSLRVLGGSSYLKQVDPASGRQTIESCCALLENWRPGQRPLLR"
     gene            complement(3041..3370)
                     /locus_tag="VFY80_06405"
     CDS             complement(3041..3370)
                     /locus_tag="VFY80_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007574.1"
                     /translation="MPDKGVIYLFRSEPDFIDDGATVTVDGNLQGTTYPGTYLRLELA
                     PGRHRISGFAGDAGWIDVAAAPGEIRFMQQTVIRPFIGPAQSHFRLVDPHYGRDAVLR
                     YELVGAR"
     gene            complement(3498..4757)
                     /gene="rho"
                     /locus_tag="VFY80_06410"
     CDS             complement(3498..4757)
                     /gene="rho"
                     /locus_tag="VFY80_06410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX6007575.1"
                     /translation="MHLSDLKNLHVGELVDMAVKNEVDGANRMRKQELIFALLKNQAR
                     KGESIYGGGTLEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDTIEGEIR
                     TPKDGERYFALVKVDKVNSEPPENSKHKILFENLTPLFPTEQLKLERDIKAEENITGR
                     IIDIIAPIGKGQRGLIVSPPKAGKTVMLQHIAHSITTNHPEVMVIVLLIDERPEEVTE
                     MQRSVKGEVVSSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMHEKRIYPAINVNRSGTRREELLIKQDILQKIWVLRKLLYPMDE
                     LEAAEFLIDKLRGTKNNADFFDSMRRG"
     gene            complement(4881..5207)
                     /gene="trxA"
                     /locus_tag="VFY80_06415"
     CDS             complement(4881..5207)
                     /gene="trxA"
                     /locus_tag="VFY80_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX6007576.1"
                     /translation="MSEHIHYVTDETFEPEVLHSTTPVLVDYWAEWCGPCKMIAPILD
                     EVAREYSGRLKVAKLNIDENQSTPPKYGIRGIPTLMLFKNGSVEATKVGALSKSQLTA
                     FIDSHI"
     gene            5402..5725
                     /gene="fdxA"
                     /locus_tag="VFY80_06420"
     CDS             5402..5725
                     /gene="fdxA"
                     /locus_tag="VFY80_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX6007577.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVAECPVEAIFAEDDVPDSQRHWTQINAELAKAWKPIIEKKPAPADADEWAKVKEKE
                     HLLEK"
     gene            complement(5759..6133)
                     /locus_tag="VFY80_06425"
     CDS             complement(5759..6133)
                     /locus_tag="VFY80_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX6007578.1"
                     /translation="MHWAAFKGFPETTKLLVERGADVNAKSNMGLTPIMQATMWGHDR
                     IVAYLLSKGARPNETDNEGLTALHNAVSDGHAKVVKVLMAAGADPHIKSAKGQTPLAV
                     AQKKNNPEILAALTSPASPVEK"
     gene            complement(6040..6666)
                     /locus_tag="VFY80_06430"
     CDS             complement(6040..6666)
                     /locus_tag="VFY80_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX6007579.1"
                     /translation="MLMHEKIARYFEGKEQFYPAALEAKYARIFNRIMDLWGMQELED
                     YFADLMVDKRGGRQGFPPDVLNDILTLSRVHGRILELQAPKQQEDDPWASDIARRSLQ
                     AEQIEFNLQGFERAVQVGNERAVKIFLKAGIKPNEKDSSGFTPLLKAASLNRTAVAAV
                     LIDAKADINVRDLQASPRCTGRRSKASRRPRSFWSSAARTSTRRATWA"
     gene            complement(6822..7793)
                     /locus_tag="VFY80_06435"
     CDS             complement(6822..7793)
                     /locus_tag="VFY80_06435"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX6007580.1"
                     /translation="MQTASRSETLARPAADPVNTLASLRVVPTGAALGAEVLGVDFSL
                     PIADEVREALRQAWADHLVLLFREQRIDDHQLIAAAGIFGPPHDSASRTYHLNAGRKI
                     DNRHMISEHPSVSIISNLDENGVPVKDNGSLGSYEVVWHTDNSYVAVPPAGSMLYALV
                     VPVNGGGDTSFNNQYLAYEELPDDLKRAIDGRCQVHDSSRNSAGVLRPGVRLPTKPEE
                     VEGPTHPLVRIHPVTKKRALYLGRRRSWPSNYIVGMNNAESEALLDRLWAHATQPRYA
                     WTHRWRVGDLVLWDNRCCMHYRSEVDSTQPRVMHRTTIKGEAPIAPW"
BASE COUNT         1412 a         2481 c         2621 g         1667 t
ORIGIN      
        1 tggcggcgcc tacggaagcc gcttcgatgg gggccctcgg ctcgatcctc gtcacggcgg
       61 ttgccggacg gatgtcgttt gcggtactgc gcgacaccgt atacgcgacg acgaagatca
      121 ccgccatggt catgttcatc ctgatctgcg cgcaggtgtt cgcgctcgcg tttcgcggcc
      181 tgcggggcga ggatttgatc cacgatctat tcgccttcct tcccggtggg gtcaatgccg
      241 acatctggtt cctgatggcg ctcatcttcg tgctaggttt tttcatcgag tggatcgaaa
      301 tcagttatat cgcggtgccc ctgtttctgc cggtattcgt gcagcagaac gtggacatcg
      361 tctggctcgc gatgctgatc tgcgtcaacc tgcagacgtc gtttctcacg ccgccgttcg
      421 gttgggcgct ttttttcctg cgcggcgtgg cgccgccgca ggtcacgacg cgtcacatgt
      481 atctcggtgt gattccgttc atcgccatgc agatcgtggg cgtcgtcttg atcttctttt
      541 atccccagat cgcgctctgg ctgccgaagg tgatcggctg gtagtcgact gctacggata
      601 gagtgggcac cggtctggtg gcgatccgtc gcttagaact tctttccgga tgtaatgacc
      661 atgatccatt ccatacgagg cacgcgcggc atggcggtcg cgccgcattc cgtcgccgcg
      721 caatccgcgc tcgatatcct gcgcgagggc ggaaatgccc tcgaggccgc gattggctgc
      781 gcggccacca tcgctgtcgt ctatccgcat atgaacggca tcggcggcga ttcgttctgg
      841 ctgatgcatg tccccgggcg caagccgcgc ggcatcgacg cctgcgggcg atccggagcc
      901 gcggcgacgc gtgattggta tgccgcgcgc ggtatcgccg atgcaatacc gtgtcgcggc
      961 gggcttgcgg cgaacacggt tgccgggacg atctccgggt gggatacggc ctttgcgctt
     1021 tcccgaaact ggggcggccg gctgccgttg aagcgcctgc tggccgatgc gatctactac
     1081 gcggagcacg gtatggcggt gacacgaagc caggcggttg cgaccgagac caaacgagcc
     1141 gagctcgagt cgcaacccgg gttcgcggcg acgttcctcc ccggcgacaa gacacccgtc
     1201 gagggcatga tgttcaggca gccgcggctg gcggaaacgc tgcgccgcct gtcgcgtggc
     1261 ctcaccggct tttaccgcgg ccggctcgcg cgctcgatgg cgcatgatct ggcggcggct
     1321 ggcagcccgc tcaccgtgga cgatctctcg gcgcaccgcg ccgcggtggt tgcgccgctc
     1381 gagcttcggc actcgcttgc aacggtgtac aacctgcccc cgcccaccca gggcgtcatc
     1441 tcgctgctga tactgggcat actcgacgcg ctgcacgtgg gcaagacagg cgcggcaagc
     1501 cccgagttca tccacctcag cgtggaagcg acgaagcgcg cattcgccct gcgcgatagg
     1561 tgcgtcacgg atcccgagta cggggcaatc gacgcccaag gccttcttgc gccggatcgc
     1621 atccgggagc acgcgtcacg ggtcgatgcc cgtaaggcag cgccgtgggg cggcggccgt
     1681 gcgccctcgg atacggtatg gctgggcgtc gttgaccaag cgggacgctc tgtcagcgtg
     1741 atccagagcg tgtaccacga gttcggctca ggcatcgttc tcgaggagag cggcgtcatg
     1801 tggcagaacc gcggctgcag cttctcgctc gaccccgatg cgctcaatac gctacagccg
     1861 ggccgcaagc cgtttcacac gctcaacacg gcactcgcgc ttttccgcga cggacgcacg
     1921 ctgctctacg gtacgatggg cggtgacggc cagccgcaga cccaggccgc ggtcttcacg
     1981 cgcatcgcgg cctttggcct caatccacag gcagcggtca gcgcaccccg ctggctgctc
     2041 ggacgcactt ggggccagac cagcgacacg ctcaaggtgg aagcgcggtt ctctggagcg
     2101 atcctaatgg cgctgggcaa attcgggcac gagatcgaag tgctgcagga ctacgacgaa
     2161 cttatgggcc acgccggtgc gatcgtgcgc aacccgagcg gcgttctgga aggcggcgcc
     2221 gatccgcgca gcgacggagc cgtcgccgcc ttctgaagtc cacaaatgaa aaaagcggcc
     2281 gtgagccgct tttttgcaca cgcaaggcgt gtcaggccgc agctttcggt gcagccttcc
     2341 gtgcgtactt ctgacggaat ttctcgactc gccccgctgt gtccacgatc ttctgcttgc
     2401 cggtatagaa cgggtgacac gccgagcaga cttccacgtg caggtcgcga ttgagcgtgg
     2461 agcgggtggt ccacgcgttc ccgcagctgc aggtgacggt gatttcgccg taatcggggt
     2521 ggatcttcgg tttcatcgct attcgtaacc ggataaacgg ggccggcgca tgggaaaggc
     2581 gcggattatg ttcgattttc gctaccgaag caagggccgc tgtccgggcc tccagttttc
     2641 gagcagcgcg cagcagctct cgattgtctg gcgtcccgag gcgggatcaa cctgcttcaa
     2701 gtatgaactt ccgcccagta ccctgagcga aacaaagtag atttctccgg gccgcgtttc
     2761 gagctcgacg ctgccggtgt cgcccgcgat tccgtgcagg cggtgctttc cggggcgaac
     2821 gtcgattctg aaataggtac cggggagcgt cgcgcccacg agtcgctcgt ccacgtagag
     2881 caccgattcc tcgtcgagtg cgttatcagg gcggaaaaca tagattgcgg cggtggccgg
     2941 gtgggtgtgg aactgctttg cttgcgcgtc gcgctcgcgc gaggcttgcg gcgtcgaggc
     3001 acatccgatg gccagcagga gtaacagcgc gatgaacggc ctaacgtgcg ccgacgagtt
     3061 catatcgcag aacggcgtcg cggccgtaat ggggatcgac cagtctgaaa tgcgactgtg
     3121 ccggcccgat gaacggccga atcacggtct gctgcatgaa gcggatctct ccgggagcgg
     3181 cggccacgtc gatccagccc gcatcgcccg cgaagccgga gatcctgtgc cggcccggcg
     3241 cgagctccaa ccgcagatac gttccgggat aggtcgtgcc ctgcaggttg ccgtccacgg
     3301 tgacggttgc gccgtcgtca atgaagtcgg gctcgctgcg gaaaaggtag atgaccccct
     3361 tgtccggcac cggctcgaag cgcttcgcct tgaggtccgc ggggctgggc gggagcggcg
     3421 cgcacgcgtg gagcgctagc agcgcgacga tcgcgataaa aagcgggaaa tccgaacggg
     3481 gcagcttcag acgattgtca gccgcgcctc atggaatcga agaaatcggc gttgttcttg
     3541 gtcccccgca acttgtcgat cagaaactcg gccgcttcca gttcgtccat gggatagagc
     3601 agcttcctca gaacccagat cttctggaga atgtcctgct tgatcaaaag ttcctcgcgc
     3661 cgcgtgccgg accggttcac gttgatcgcg gggtagatcc gcttctcgtg catgcgccgg
     3721 tcgagatgga tctccatgtt gccggtgcct ttgaactctt catagatcac gtcgtccatg
     3781 cggctgccgg tgtcgatcag cgccgtggcg atgatggtaa gcgagccgcc ttcttcgatg
     3841 ttgcgcgccg caccgaagaa acgcttgggg cgctgcaatg cattcgcatc gacaccgccg
     3901 gtcagcacct tgccggacgc aggcaccacg gtattgtagg cgcgcgcgag acgggtgatg
     3961 gagtcgagca ggatcacgac gtccttcttg tgctcgacca gccgcttcgc tttttcgatg
     4021 accatctcgg cgacctgaac gtgccggctt gccggttcgt cgaacgtcga ggagacaacc
     4081 tcgcccttga ccgagcgctg catctcggtc acctcttcgg gccgctcgtc gatcaggagc
     4141 acgatcacca tcacttccgg atggttcgtc gtgatcgagt gcgcgatgtg ctgcagcatg
     4201 accgtcttac ccgccttcgg cggcgacacg atgaggccgc gctggccctt gccgatcgga
     4261 gcaatgatgt cgatgatgcg gccggtgatg ttttcctcgg ccttgatgtc gcgctcgagc
     4321 ttgagctgct cggtgggaaa aagcggtgtc aggttctcga acaggatctt gtgcttggaa
     4381 ttttccggcg gctcggaatt gaccttgtcc actttgacga gcgcgaaata gcgctcgccg
     4441 tccttggggg tgcggatctc gccctcgatc gtgtccccgg tatggaggtt gaagcgccgg
     4501 atctggctcg gagacacgta gatatcgtcg gtgccggcga ggtaggacgt gtccggtgag
     4561 cgcagaaagc cgaaaccgtc gggcagcact tcgagcgtgc cgccgccata gatgctctcc
     4621 cccttgcgtg cctgattctt gagcagcgcg aagatgagct cctgcttgcg catgcggttc
     4681 gcaccgtcga cttcgttctt gacggccatg tcgacgagct cgcccacgtg gagattcttg
     4741 aggtcggata gatgcatatg cggagagcgc tacgttacgt gagggaagga aaaccgaggg
     4801 gactgcaccc gcgcccgaac cgcccattct ttctttatga ggggcggcct tcgtcaggcg
     4861 cggaaaaaag cgtatagggt ttatatatgg ctgtcaataa atgcggtcag ttgagatttc
     4921 gacagcgcgc ccaccttggt tgcctccacc gagccgttct tgaacagcat gagcgtcggg
     4981 ataccgcgaa tgccatactt gggcggggtg gactggttct cgtcgatgtt gagcttcgcc
     5041 actttaagcc tgccggagta ctcacgggcg acttcatcca gtatcggcgc gatcatcttg
     5101 cacggtccgc accactccgc ccagtaatcc accaggacgg gggtcgtcga atgcagaacc
     5161 tcgggttcga acgtctcgtc agtgacgtaa tggatatgct cactcatgga tgggccttgg
     5221 aaaagtttgg aggaaacgcc acacggaggg catggcgtca ggaaacttcg ggttatgcta
     5281 accaaatttt gtcaccttgg gaagcaaacg ctcggctgtc ggcgttcatc cgaccccgac
     5341 gcttctgcta aaatgagcgc cttcctcgat ttttcaagac ccggttttcc gaaggaatac
     5401 tatgacttac gttgtcaccg agagctgcat caaatgcaag tataccgatt gcgttgatgt
     5461 gtgcccggtg gattgtttcc gcgaagggcc caacatgctg gtgatcgacc ccgacgagtg
     5521 catcgattgc acactttgcg ttgcggagtg cccggtcgaa gcgatattcg cggaggacga
     5581 tgtgcctgac tcgcagcgcc actggacaca gatcaacgcg gaactggcga aggcctggaa
     5641 gcccatcatc gagaaaaagc ccgcgcccgc ggatgccgac gaatgggcca aggtgaagga
     5701 gaaggagcac ctgctggaga aatgatgcgc aaggcgccag ccgctcgcag cgggtgatct
     5761 atttctcgac cgggctcgcc ggcgaggtga gcgctgccag gatttccgga ttgttcttct
     5821 tctgagcgac ggccaagggg gtctgcccct tcgccgactt gatgtggggg tcagcgcctg
     5881 ctgccatcag caccttcacc accttagcgt gtccgtcgga cacggcgttg tgcagcgcgg
     5941 tcaatccctc gttgtccgtt tcgttcgggc gcgcgccctt ggagagcagg taggcgacga
     6001 tgcgatcgtg gccccacatc gtggcctgca tgatcggcgt caagcccatg ttgctcttcg
     6061 cgttgacgtc cgcgccgcgc tcgaccagaa gcttcgtggt ctccgggaag cctttgaacg
     6121 ccgcccagtg cagcggggtg aagcctggag atcgcggacg ttgatgtcgg ccttggcatc
     6181 gatgagcacc gccgccacgg cggtgcgatt gagggaggcc gccttgagca agggcgtgaa
     6241 gccgctgctg tccttctcgt ttggcttgat acctgctttg agaaagatct tgactgcgcg
     6301 ctcgtttcca acctgaactg cccgttcgaa gccctgtaga ttgaattcga tctgctccgc
     6361 ctgcagcgac cgccgcgcga tgtcgcttgc ccacgggtcg tcttcctgct gcttcggcgc
     6421 ctggagctcg agtatgcgtc catgaacccg ggagagcgtg aggatgtcgt tcagcacgtc
     6481 cggcggaaag ccctggcggc cgccacgctt gtccaccatg agatccgcaa aatagtcctc
     6541 cagctcctgc attccccata agtccatgat ccggttaaag atcctggcgt acttcgcttc
     6601 cagagcggcg ggatagaact gctccttgcc ttcgaaatac ctggcaattt tttcatgcat
     6661 cagcatgggg ggacctctct tgcagggccg gttcttataa gtttgcggga cgcattttgc
     6721 tcgcaaggat tgtaacggtt tggcgagcgg gtgtgctgat taaaaagcca catctccccc
     6781 gaaatggaga caggcgacgg ggcgcggact ggagcgctgg cctaccacgg tgcgatgggg
     6841 gcttctccct tgatggtcgt gcggtgcatc acgcgtggtt gggtggaatc gacctcgctt
     6901 cgataatgca tgcagcagcg gttgtcccag aggacgaggt cacccactcg ccagcgatgc
     6961 gtccaggcat accgcggttg cgtggcgtgc gcccacagcc ggtcaagcaa tgcctcgctt
     7021 tccgcattgt tcatacccac gatgtagttc gacggccatg accgacgccg cccgagatag
     7081 agtgcgcgct tttttgtgac cggatgaatg cgcacgagcg gatgggtcgg gccttcgact
     7141 tcttccggct tggtgggcaa cctgacgccc gggcgcaaca cgccggcgct gttgcggctc
     7201 gaatcgtgca cctgacacct cccgtcgatg gcccgcttca gatcgtccgg caactcctcg
     7261 taggcgagat actgattgtt gaaggacgtg tcgccaccgc cgttcaccgg cacgacgagc
     7321 gcgtagagca tgctgccggc aggcggtacc gccacgtacg aattgtcggt gtgccacacc
     7381 acttcgtagc tgcccaagct gccattgtcc ttcaccggca cgccgttctc gtcaagattc
     7441 gagatgatgc tgacgctcgg gtgctcggaa atcatgtggc ggttatcgat cttcctgccc
     7501 gcgttgaggt gatacgtccg cgatgcagaa tcgtgtggcg gaccgaagat gccagccgca
     7561 gcaatcagct ggtggtcgtc gatgcgctgc tcgcggaaga gcagcacgag gtgatcggcc
     7621 cacgcctggc gcaacgcctc gcgtacctca tcggcgatcg ggagcgagaa atcgacgccc
     7681 aggacttcag cgccgagcgc ggcgccggtc ggcacgacgc gcagcgaagc aagcgtgttg
     7741 acggggtcgg ctgcgggcct ggcaagggtt tcacttcggc ttgcggtttg catgcgaggc
     7801 tcctcgtggg gcgttgagcg attctgtgcg cggtgaatgg agagcgtcaa gcggcgcatc
     7861 ctgcagggcg cctcgcacta cgctgccggt gacacgcccg ggagtgctca ccgcgcaagc
     7921 cgggatacgg gctgtcgatc ccacgttgca gccgctgccg cctgctctcg ctgatatgcg
     7981 tcgccggcgt ccgcgtgttt ccggatccac ttccgacgca aattcgctga tgtgtcgctc
     8041 acgacggcga gccgacgcgc gcactctcgg agagccggcg cggttctctc agcggaggta
     8101 ggcttcgatc gcgtaatgcc gcagcatgcg gtggctgttg aaaaaggacg cattcttgcc
     8161 gatcgcgccc ttcatcacac c
//
LOCUS       DASURG010000366         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1392291, whole genome shotgun sequence.
ACCESSION   DASURG010000366 DASURG010000000
VERSION     DASURG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392291"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..972
                     /locus_tag="VFY80_06440"
     CDS             85..972
                     /locus_tag="VFY80_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6007581.1"
                     /translation="MSPDRHPRISVVILTHNRADEAIRTVAHMRELAERPRLVVVDNA
                     SSDGTAERITARFPEVTLIRLPRNIGAAARNVGVKEVETPYVAFCDDDTIWAPGSLAH
                     ACEVLDAWPGIAVLTAHVLVGEAWRDDPACAMMDASPLPSHGLPGKAVLGFLAGACVF
                     RRERFLESGGYEPRFLIGGEEALLAIDIVARGHVIVYVRALTVHHQPSTRRNVRERQR
                     HTVRNALWVAWLRRPLWSALARSADILKRAGFTDAALCGFVDAARELPWVLRERRVIP
                     PSVEALCALLEKRAPLRPS"
     gene            complement(967..1440)
                     /locus_tag="VFY80_06445"
     CDS             complement(967..1440)
                     /locus_tag="VFY80_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007582.1"
                     /translation="MDASLHHRIDPELARHALAFLAPLMERAVSDSAIGQSGVLYVVV
                     MHPGRPAGRFKFEDAILYEEAYGKPRNEWDADYADYARKKARVSWSSGARSGDPCTVP
                     RWLDLGQATALPGGMVVEDTVVAASGANPVYDEVLAGAVALSLRAAIMLRAEAAS"
     gene            complement(1476..>3156)
                     /locus_tag="VFY80_06450"
     CDS             complement(1476..>3156)
                     /locus_tag="VFY80_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6007583.1"
                     /translation="DIRDAPSSRPLEVGGAEVRFNHVSFGYDPSRRVLHDIAFRIAPG
                     ETVAVVGGSGSGKSTIARLLLRFHEASDGSIQVDGQDIRHVTQKSLRDAIGIVPQDTV
                     LFNETIYFNIAYGHPDATREEVLAAAQAARVHDFVTALPEQYDTVVGERGLKLSGGEK
                     QRIAIARAILKNPPIIIFDEATSALDARAEQAIQAELDRVAESRTTLVIAHRMSTIVG
                     ANEILVLEHGRIVERGTHATLLEHGGIYAQMWSVQQQERELERVQRKLALQPINLSTV
                     VAAVIDGLRGEIDSSGINLYAVVSDARVVGDPSLIQQAVWDICEHAIRAAGAGGRVEV
                     RVDETATEARLSVTDTGSADAAVQRGAWRGAATARARDVLRARAIAEQHGGHLVVVKV
                     TDVPGSTYTMTLPLRRALPPLVARAQLALVPEAMPEQAGIGDLKVVLIDGDEDRADLT
                     TLLERCGARVQAYGRAADALRALESFNEPEWPDLLISDIALPDEDGYSVIRQVRALEE
                     TRQTGLSARTPAIALGTEEDSTRALLAGYQMQLSKPVNPQALLKAVRLLAA"
BASE COUNT          501 a         1071 c         1033 g          551 t
ORIGIN      
        1 gtccgccggg cgcccgggac atctcgatgc attgacggcc gagcgctact ggccgctcgc
       61 tgtggaagag gagacgttgc acggatgagt cccgaccgcc atccgcggat ctcggtcgtc
      121 atcctgacgc acaatcgggc tgacgaggcg atccgtaccg tcgcgcacat gcgtgaactg
      181 gccgaacgac cgcgccttgt cgtcgtcgac aacgcatcga gtgacggcac ggccgagcgg
      241 atcaccgccc gatttccgga ggtgacactg atccggcttc ccaggaacat cggcgccgcg
      301 gcgcgcaatg tcggtgtaaa ggaagtggag acgccctatg tcgccttctg cgacgacgac
      361 acgatctggg cgccgggctc gctcgcgcac gcctgcgagg ttctcgacgc atggcccggc
      421 atagccgtgc ttacggctca cgtgctggtc ggcgaggcat ggcgcgatga cccggcgtgc
      481 gcaatgatgg atgcgagccc gctcccctcg catggtcttc ccggaaaggc cgtgctcggc
      541 ttcctcgctg gcgcgtgcgt tttccgtcgc gagcgatttc tggaaagcgg aggctacgag
      601 ccgcgtttcc tgatcggtgg cgaggaagcg ctgctcgcca tcgacatcgt ggcgcggggg
      661 catgtcatcg tatacgtccg agccctgacc gtgcatcacc agccttcgac tcggcgcaac
      721 gtcagggagc ggcagcgcca cacggtgaga aacgcattat gggtcgcttg gctgcgtcgg
      781 ccgttgtgga gcgcactcgc ccgcagcgcc gacatcctca agcgcgccgg ctttaccgac
      841 gcggccctgt gcggctttgt cgacgccgcg cgcgaattgc cgtgggtgct gcgcgagcgg
      901 cgcgtcatcc ccccgagcgt cgaggcgctg tgcgccttgc tggagaagcg cgcaccgctc
      961 cgcccctcat gatgcagctt ctgcgcgcaa catgatggcg gcacgcaggc tcaacgcgac
     1021 cgcgccggcg agcacttcgt cataaaccgg gttcgcgccg ctcgctgcaa ccacggtgtc
     1081 ttccaccacc atgccgccgg gaagcgcggt ggcctgacca aggtcgagcc agcgggggac
     1141 ggtacacggg tcaccactgc gcgctcccga agaccagctg acgcgcgctt tcttccgcgc
     1201 gtagtcggcg taatcggcat cccattcatt gcgcggcttg ccatacgcct cctcatacag
     1261 gatcgcgtcc tcgaacttga aacggccggc cgggcggccg ggatgcatga cgacgacgta
     1321 gagcacgccg ctctgtccga ttgccgagtc ggataccgcc cgctccatga gcggcgccag
     1381 aaatgcgagg gcgtgccgcg caagttccgg atcgatccga tgatgtagtg aggcatccat
     1441 ggcaattcct ccttaaagtg ggtaggccca atacgtcacg ccgcgagaag ccgcaccgcc
     1501 ttcagtagcg cttgcgggtt caccggcttc gaaagctgca tctggtatcc cgccagcagg
     1561 gcacgggtgc tgtcctcttc ggtgccgagc gcgatggccg gcgtgcgggc ggagagtccc
     1621 gtttgacgcg tttcctccag cgcgcgaact tggcggatca cgctatagcc gtcctcgtcc
     1681 ggcagtgcga tatccgagat gaggaggtcg ggccactccg gttcgttgaa agactccagc
     1741 gcgcgcagcg catccgctgc gcgaccatac gcctgcacac gcgccccgca gcgctcgagg
     1801 agtgtcgtga ggtcggcgcg atcctcatcg ccgtcgatga gcaccacctt gagatcgccg
     1861 atgcccgcct gctcgggcat cgcttcaggg accagcgcga gctgcgcccg cgcaacgagc
     1921 gggggcaatg cccgccgcaa cgggagcgtc atggtgtagg tgcttcccgg cacgtcggtc
     1981 actttgacga cgacgaggtg cccgccgtgc tgctcggcaa tggcgcgcgc gcggagcaca
     2041 tcgcgtgccc gcgccgttgc cgcacctcgc cacgcccccc gttgcacggc ggcatccgcg
     2101 ctgccggtat cggtgacgga tagtcgagcc tctgtggccg tctcgtccac gcggacctcc
     2161 acgcgcccgc ccgctccggc ggcccggatg gcgtgctcgc agatatccca caccgcttgc
     2221 tggatcaagc tcggatcgcc tacaactcgg gcgtccgaga cgacggcgta aagattgatt
     2281 ccgctcgagt cgatttcgcc gcgcagaccg tctatcaccg ccgccacgac tgtggaaaga
     2341 ttgatcggct ggagcgcgag cttgcgctgc acgcgctcga gttcccgctc ctgctgctgc
     2401 accgaccaca tctgcgcata gatgccgccg tgctcgagaa gcgtcgcgtg agtcccgcgc
     2461 tccacgatcc gcccgtgctc cagcacgagt atctcgtttg ctcctacgat ggtggacatc
     2521 cgatgcgcga tgacaagagt ggtgcggctt tccgcgacac gatcgagttc ggcctggatc
     2581 gcctgctcgg cgcgggcgtc gagcgcggag gtggcctcgt cgaagatgat gatgggcgga
     2641 ttcttgagta tcgcccgggc gatcgcgatc cgttgcttct cgccgcccga gagcttgagg
     2701 ccgcgctcgc ccaccacggt atcgtactgc tccggaagcg ccgtgacgaa gtcgtgaacg
     2761 cgcgcagcct gcgcggccgc gagcacttcc tcgcgcgttg catcgggatg cccgtaggcg
     2821 atgttgaagt agatcgtctc gttgaagagc acggtatcct gcggcacgat gccgatcgcg
     2881 tcgcgcaggc tcttctgtgt cacgtgccgg atgtcctgcc catccacttg gatgctgccg
     2941 tcgctcgcct cgtggaaacg cagcagcagg cgcgcgatgg tggacttgcc cgaaccgctg
     3001 ccgcccacca cggccaccgt ctcgccgggc gcaatgcgaa atgcaatgtc gtgcaatacg
     3061 cgccggctcg ggtcgtagcc gaagctcaca tggttgaaac gcacttctgc gccgcctacc
     3121 tcgagcgggc gggacgaggg cgcgtcgcgg atatcc
//
LOCUS       DASURG010000367         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_983123, whole genome shotgun sequence.
ACCESSION   DASURG010000367 DASURG010000000
VERSION     DASURG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983123"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFY80_06455"
     CDS             <1..1220
                     /locus_tag="VFY80_06455"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX6007584.1"
                     /translation="RDLRLMLLGARRLGVPLLIGSAGTGGNDLQLDLMYRLVREVAAA
                     EGLEFRLALIHAEQDKAYLKKRYREGRIKPLSPAPPFDEIVIERSAHIVGMMGAEPFQ
                     RALEAGAEVVIAGRSSDTAIFAALPLLRGFPDGMAWHAAKILECGAAAVVNRRTPDCM
                     FAWVRQDHLVVEAPDPTLRCTPQSIASHSLYENADPFRLVECSGTLDLTDSRYEALSA
                     RAVKVSGSSFIPAERYTVKLEGAEQAGYQSILIGSIRDPYIIRQIDDWIARLKERIAN
                     RVRMVYGSTLGENDYILHIRVYGKDGTMGPLEPVKVIRSHELCLVFEATAPTQEVAGT
                     IAGIIRHQALHLPIPEWSGLITAVACPYNPIDRGAVYRFNVNHVVEPDDPCEMFPMEL
                     YEVKRDAHRKLAA"
     gene            1217..1525
                     /locus_tag="VFY80_06460"
     CDS             1217..1525
                     /locus_tag="VFY80_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEX6007585.1"
                     /translation="MTLLAELARLIRSKNAGPFELTFDIMFDDAPTYERVKRSGAVTR
                     ESIAARYGLEPGKVKFFLCDHALAIKASIPRPYFQGDPRDSDGHGGQQYAPLMDIEIP
                     "
     gene            1569..>2107
                     /locus_tag="VFY80_06465"
     CDS             1569..>2107
                     /locus_tag="VFY80_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007586.1"
                     /translation="MKARAAVIFCAALATVAAVPVSAAPAVAAKDYPNKPVRFIVPFV
                     AGAGTDMTARAIAAKLSERWGQQFVVDNRPGAAGTIGVDITAQATPDGHTICLISASH
                     SVNAATNPKLPYDLTRDLQAVSQATSLFYVMYVNPSVPAKSVQELVAHAKANPGKLNF
                     GSSGTGGLQHFAGEMLAHLA"
BASE COUNT          397 a          683 c          665 g          362 t
ORIGIN      
        1 gacgtgatct gcggctgatg ctgctcgggg cgcggcggct cggggtgccg ctactgatcg
       61 gctcggctgg cacgggtgga aatgacctgc agctagacct gatgtatcgg ctcgtgcgag
      121 aggttgcggc ggcggaggga ctcgaattcc ggctcgctct gatccacgcc gaacaggaca
      181 aggcctacct caagaaacgc tatcgcgaag ggcgcatcaa gcccttgtct cccgcacctc
      241 ccttcgatga aatcgtcatc gagcgcagcg cgcatatcgt gggcatgatg ggtgccgagc
      301 cgtttcagcg ggcgctggaa gcaggcgccg aagtggtcat cgcagggcgc tcgagcgaca
      361 cggcgatctt cgccgcgctt ccgctcctgc gcggtttccc ggacggcatg gcgtggcacg
      421 cagcgaaaat cctcgaatgc ggcgcagcgg cggtggtaaa ccgcagaacg ccggattgca
      481 tgttcgcatg ggtgcgtcag gaccacttag tcgtcgaggc gccggatccg acgctgcgct
      541 gcacgccgca gagcatcgcc tcgcacagct tgtacgaaaa cgccgatccg ttccgcctcg
      601 tggaatgctc cggcacgctc gaccttaccg actcacgtta cgaagcactg agtgcacgcg
      661 cggtgaaagt gtccggcagc agcttcattc cggccgagcg ctataccgtc aagctggaag
      721 gcgcggaaca ggcgggctac cagagcatct tgatcggctc gatccgcgac ccttacatca
      781 tccggcagat cgacgactgg atcgctcgat tgaaagagcg catcgcaaac cgcgtgcgca
      841 tggtgtatgg gtcgacgctc ggcgagaacg actatatcct gcacatccgc gtctacggca
      901 aggacggaac catgggaccg ctcgaaccgg tgaaggtgat ccgctcgcac gagctgtgtc
      961 tcgtgttcga ggcgacggcg cccacgcagg aagtggcggg caccatcgcc ggcatcatcc
     1021 gccaccaggc gctgcatctg ccgataccgg agtggagcgg gctcatcacg gcggtggcgt
     1081 gcccctacaa tccgatcgac cgcggcgccg tctatcgctt caacgtgaat cacgtcgtgg
     1141 agccggacga tccctgcgag atgttcccga tggagctcta cgaagtgaag cgcgatgctc
     1201 acagaaagct cgccgcatga ccctgcttgc cgagctcgcg cgcttgatcc gcagcaagaa
     1261 tgcggggccg ttcgagctca ccttcgacat catgttcgac gatgcgccca cctacgagcg
     1321 cgtcaagcgc tccggcgccg ttacgcgcga atcgattgca gcgcgctacg ggctcgagcc
     1381 cgggaaggtg aagttctttc tgtgcgatca cgcgctcgcg atcaaggctt cgatcccgcg
     1441 tccgtacttt cagggcgacc cgcgcgacag cgacgggcac ggcgggcaac agtatgcgcc
     1501 gctcatggac atcgagattc cgtgagacag gcgacacgac agtaccgaga gacacgagga
     1561 ggggaatcat gaaagcacga gcggccgtca tcttctgcgc ggcgttagcg acggttgcgg
     1621 cagttccggt ttccgcagcg cccgccgttg cagcgaagga ctatcccaac aagccggtcc
     1681 gtttcatcgt gccgttcgta gccggtgccg gcaccgacat gacggcgcgc gccatcgccg
     1741 cgaagctttc cgagcggtgg ggccagcagt tcgtcgtcga caaccgtccc ggcgcggccg
     1801 gcaccatcgg cgtcgatatc actgcgcagg caacgcccga cggccatacg atctgtctca
     1861 tctcggcatc gcattcagtg aacgcagcca ccaatccgaa actgccctac gatctgacgc
     1921 gcgacctcca ggccgtctcc caggcgacgt cgctcttcta cgtgatgtac gtcaatcctt
     1981 cggtacccgc gaaatcggtg caggagcttg tcgcgcacgc caaggcgaat cccgggaagc
     2041 tcaacttcgg ctcatcgggc accggcggcc tccagcactt cgcaggcgag atgttggcgc
     2101 accttgc
//
LOCUS       DASURG010000368         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_472206, whole genome shotgun sequence.
ACCESSION   DASURG010000368 DASURG010000000
VERSION     DASURG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472206"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFY80_06470"
     CDS             <1..254
                     /locus_tag="VFY80_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HEX6007587.1"
                     /translation="ASDDMPSILDVLSISLNIMQVRITRSRLAGEPADVMIRPRLASF
                     ALLDYHRAEPAIAEGKRAAEQVLPELRELFGIRGFSPRA"
     gene            complement(264..>2091)
                     /gene="ftsH"
                     /locus_tag="VFY80_06475"
     CDS             complement(264..>2091)
                     /gene="ftsH"
                     /locus_tag="VFY80_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6007588.1"
                     /translation="VVKGNVSAIYSKGTSIEGRFAAPIKWPPSDEPKDVKKLPSAPRD
                     SSGSQPRQADTFTTELPAFVDPGLEQFLIDHKVEISALPIKPGSPWATLLFGFGPALL
                     IILFYVWLYRRASQAGGMGGALMGIGRSKARRYDASSESKVTFNDVAGIDEAENELVE
                     IVDFLKSPEKYTRLGGTAPKGVLLVGAPGTGKTLLARAVAGEAGVPFFSMSAAEFVEM
                     IVGVGAARVRDLFKQARENAPAIIFIDEIDSIGRARGPTAFGASSEQEQTLNQILTEM
                     DGFSSREGVIVLAATNQPDLLDRALLRPGRFDRRVVVNLPDKNGREAILKVHTRNVPL
                     ATDVNLTDLAAATPGLSGADIRNLVHEAALLAARRERNEVRQNDFLDALEKIVLGPER
                     PLLLSRDDRERIAYHEGGHAILGLVVPGADPVHRVSIVPRGQALGVTYQRPESDRYNY
                     PEAYLRARIIGMLGGRAAEEIVYGTRTTGAENDIEQVTNLARNMVTRWGMSDKVGMIQ
                     LAPRQNPYLATAMGYGGDKPFSEETARVVDAEVHRIVNECHTEAVRLLTKHRKQLDAL
                     VAALLTRETLGEDEILEVTGLPPAPALESRRIDAPTEAVQRA"
BASE COUNT          322 a          698 c          674 g          397 t
ORIGIN      
        1 ccgcttcgga cgacatgccc tccatcctcg atgtgctcag catcagcctt aacatcatgc
       61 aggttcgcat cacgcggagc cgactggccg gagagccggc cgatgtcatg atccgcccgc
      121 ggctcgcgag cttcgcattg ctggactatc accgtgccga gcccgcgatc gcggaaggta
      181 agcgcgccgc cgaacaggtg ctgccggagc tgcgggagct gttcggaatc cgcggctttt
      241 cccctcgggc gtagcgcacg cgcttacgca cgctgcaccg cctcggtggg cgcgtcgatt
      301 cgacgggatt cgagcgcagg ggccggcggc agtccggtca cttccaggat ctcgtcttcg
      361 cccagcgtct cgcgcgtgag cagtgcggcc accagcgcat cgagctgctt gcgatgtttg
      421 gtcagcagcc gcaccgcctc agtgtggcat tcgttgacga tgcggtgcac ttcggcatcg
      481 acgacccggg cggtctcctc gctgaaaggt ttgtcgccgc cgtatcccat cgcggtggcg
      541 aggtagggat tctgacgcgg cgcaagctgg atcatcccga ctttgtcgct catgccccag
      601 cgggtgacca tgttgcgcgc gagattggtg acctgctcga tgtcgttttc ggcgccggtg
      661 gtgcgcgtgc cgtacacgat ttcctccgca gcgcgtccgc cgagcatgcc gatgatgcgc
      721 gcgcgcaggt acgcttccgg gtagttgtag cggtcggatt ccgggcgttg atacgtgact
      781 cccagcgcct ggccacgcgg gacgatgctc acccggtgca cgggatccgc ccccgggacc
      841 accagtccga ggatggcgtg gccaccctcg tggtaggcga tgcgctcgcg atcgtcgcgg
      901 ctcagaagca gggggcgctc cggccccagc acgatcttct ccagcgcatc gaggaagtcg
      961 ttctggcgga cctcgttccg ctcgcgccgt gccgcaagca gcgccgcttc gtggaccaga
     1021 ttcctgatgt cggcccccga gaggcccggg gtggcggccg cgagatcggt gagattcacg
     1081 tcagtagcga gcggcacgtt gcgcgtgtgc actttcagga tcgcctcacg cccgttcttg
     1141 tcgggcaggt tcacgacgac gcgccggtcg aatcgtcccg gtcgcagcaa ggcgcggtcg
     1201 agcagatccg gttgattggt ggctgcgaga acgatgacgc cttcacggct ggaaaaaccg
     1261 tccatctcgg tgaggatctg gttcaacgtc tgctcttgtt cactcgacgc accgaatgcg
     1321 gtcgggccgc gcgcacggcc gatggagtcg atctcgtcga tgaatatgat cgccggtgcg
     1381 ttttcgcgcg cctgcttgaa aagatcgcgg acgcgagccg cgccgacccc cacgatcatc
     1441 tccacgaatt cggccgcgct catcgagaag aacggcacgc ccgcttcccc ggcgactgct
     1501 ctcgcgagca gcgtcttgcc ggtgcccggc gcaccgacca gcagcacgcc tttcggcgcc
     1561 gttccgccga ggcgcgtgta cttttcgggc gacttcagga aatcgacgat ttcgacgagc
     1621 tcattttctg cctcgtcgat tccggccacg tcgttgaagg tgaccttgct ttccgacgac
     1681 gcgtcgtagc gccgcgcctt gctgcgtccg atgcccatca gcgcgccacc catgccgccc
     1741 gcctgcgatg cgcgccggta gagccacacg tagaacagga tgatcagcag cgcggggccg
     1801 aaaccgaaca atagcgtcgc ccacgggctg ccaggcttga tcggcagcgc actgatctcg
     1861 accttgtgat cgatcaggaa ctgctccagt cccggatcga cgaaagcggg cagctccgtg
     1921 gtgaacgtat cggcctgccg cggctgtgat ccgctgctgt cccgcggggc cgacggcaat
     1981 ttcttgacat ccttgggctc atcggacggt ggccacttga tcggcgccgc aaaccggcct
     2041 tcgatgctgg tgcccttgct gtagatcgcc gacacgttgc ctttcacgac c
//
LOCUS       DASURG010000369         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_677223, whole genome shotgun sequence.
ACCESSION   DASURG010000369 DASURG010000000
VERSION     DASURG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677223"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /gene="dapA"
                     /locus_tag="VFY80_06480"
     CDS             <1..711
                     /gene="dapA"
                     /locus_tag="VFY80_06480"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX6007589.1"
                     /translation="HLLIKTAIDHAAGRLPVIAGTGANSTREAIELAAYAKQVGANMS
                     LSVVPYYNKPTQEGLYRHFRAIAEAVSIPQIVYNVPGRTVADLSNDTMLKLAQIPNIV
                     GIKDATANLERGADLLRRKPRDFRVFSGDDATGLALMLLGGHGVISVTANAAARLMHE
                     MCAAALAGDIGRAREINNRLLGLHRQLFCEANPIPVKWVVQQMGLIKGGIRLPLTPLS
                     EGFIPQVREAMRQAGIAQ"
     gene            708..1826
                     /gene="bamC"
                     /locus_tag="VFY80_06485"
     CDS             708..1826
                     /gene="bamC"
                     /locus_tag="VFY80_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX6007590.1"
                     /translation="MMRKALGCAVAALALAACSSFEVPTKKIDYKSAGKTKPLEVPPD
                     LIAPSTDDRYRVPDVNPRSGATFSAYNRERSGPQAASASPSVLPNISDARIERAGTQR
                     WLVVKGEPTALWQVVKDFWQETGFIVNVEIPEAGVMETDWAENRAKIPDGFIRNTLGK
                     LLDSVYSTSERDKFRTRLERGAESGTTEIYVSHRGMEEVFTTSQKEETKWQPRPPDPE
                     LEAEMLRRLMVRFGVQEQRAKQQLDAAGTAVARATLERGQGTLVLNEQFDRAWRRVGL
                     ALDRVGFTVEDRDRSKGLYYVRYIDPQIDNRSAENKGWLSKLKFWGNDDKPKQEQYRI
                     QVKGGEEGAEVNVLNKEGTRDRSETSGRILSLLYEQLK"
     gene            1836..>2101
                     /locus_tag="VFY80_06490"
     CDS             1836..>2101
                     /locus_tag="VFY80_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6007591.1"
                     /translation="MRFASLGSGSQGNALIVEAGSTRLMLDCGFGIGECTFRLERLGL
                     EPSDLDAILVTHEHDDHIGGVARFARKYELPVYLTYGTLTALNG"
BASE COUNT          415 a          673 c          682 g          331 t
ORIGIN      
        1 cacctgctga tcaagacggc aatcgatcac gccgccgggc gccttccagt catcgccgga
       61 acgggcgcga attcgacgcg cgaggcgatc gagcttgccg cctacgcgaa gcaggtcggc
      121 gctaatatga gcctgtcggt cgttccctac tacaacaagc ccacccagga agggctctat
      181 cgtcacttcc gggcgattgc cgaggccgtg agcattccgc agatcgtcta caacgttccg
      241 ggacgcacgg tcgccgatct ttcgaacgac acgatgctca agctcgcgca gattccgaac
      301 atcgtcggca tcaaagacgc aaccgccaat ctcgagcgcg gcgcggatct cctgcgccgc
      361 aagccgcgcg attttcgggt gttcagcggc gacgatgcca cggggctcgc gttgatgctt
      421 ctcggcgggc acggcgtgat ctccgtcacc gccaacgccg cggcccgact catgcacgag
      481 atgtgcgctg ccgcgctcgc tggagatatc ggccgcgcgc gcgaaatcaa caaccggctc
      541 ctcggcctgc atcgccagct cttctgcgaa gcgaatccga ttcccgtgaa gtgggtggtg
      601 cagcagatgg gcctgatcaa aggagggatc cgtctgccgc tgacgccgct gtccgaggga
      661 ttcattcccc aggtgcgcga agcgatgcgg caggcgggga tcgcgcaatg atgcggaaag
      721 ctctgggatg tgcggtggcg gcgctggcgc tcgcagcctg cagttcgttc gaagtcccca
      781 ccaagaaaat cgattacaag tcggccggca agacgaaacc gctcgaagtg ccgcccgatt
      841 tgatcgcccc gagcacggac gaccgctacc gcgtgccgga cgtgaacccg cgcagcgggg
      901 cgaccttctc ggcctataac cgcgagcgga gcggacccca ggcggcttcc gcttcgccca
      961 gcgtgctgcc caacatcagc gatgcccgga tcgagcgggc gggcacgcag cgctggcttg
     1021 tggtgaaggg cgagcccacc gcgctgtggc aggtcgtcaa ggatttctgg caggaaaccg
     1081 gcttcatcgt gaacgtcgag atcccggaag cgggggtgat ggagaccgac tgggcggaga
     1141 accgcgcgaa gatccccgac ggcttcattc gcaacacgct cggaaaactc ctcgattcgg
     1201 tgtattcgac ttccgagcgc gacaagttcc gcacgcggct cgagcgcggc gccgagtccg
     1261 gcacgacgga aatctacgtc agccatcgcg ggatggagga agtcttcacc acgtcgcaga
     1321 aagaggagac gaagtggcag ccacgcccgc cggatcccga gctcgaggcg gaaatgctgc
     1381 gccggctgat ggtgcgcttc ggcgtgcagg aacagcgcgc gaagcagcag ctcgacgctg
     1441 cgggcactgc agtagcgcgt gcgacacttg agcgcggaca gggcacgctc gtgctgaacg
     1501 agcagttcga tcgcgcgtgg cggcgcgtcg gccttgcgct cgaccgcgtg ggcttcacgg
     1561 tggaagaccg agaccgctcg aaaggcctct actacgtgcg ctacatcgat ccccagatcg
     1621 acaacagaag cgccgagaac aaaggctggc tctcgaagct caaattctgg ggcaacgacg
     1681 acaagccgaa gcaggagcaa taccgcatcc aggtgaaagg cggggaggag ggcgcggaag
     1741 tgaacgtcct caacaaggaa gggacgcgtg accggtcgga aacctcgggc cgcatccttt
     1801 cgctgctcta cgaacaactg aaatagcccg cggtgatgcg tttcgcatca ttgggcagcg
     1861 gcagtcaggg caatgcactg atcgtcgagg cgggctcgac gcgcctcatg ctcgactgcg
     1921 ggttcggcat cggcgaatgc accttccgcc tcgagcgtct cgggctcgag ccctccgatc
     1981 tcgacgccat cctcgtcacc catgagcacg acgaccacat cggtggcgtt gcgcgtttcg
     2041 cgcgcaaata cgagctgccg gtctatctga cctacgggac gctcaccgcg ctcaacggcg
     2101 a
//
LOCUS       DASURG010000370         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_472301, whole genome shotgun sequence.
ACCESSION   DASURG010000370 DASURG010000000
VERSION     DASURG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472301"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="argH"
                     /locus_tag="VFY80_06495"
     CDS             complement(<1..830)
                     /gene="argH"
                     /locus_tag="VFY80_06495"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX6007592.1"
                     /translation="MAANDRPPAGRSKPWSGRFREPITDLVQRFTASVSFDQRLAEFD
                     IDGSLAHARMLCAAGILSAADLAAITKGMEQLRGEVRAGTFPWSLEYEDVHFNIERRL
                     TDLIGDAGKRLHTARSRNDQVATDVRLYLRASIDHVLTLIRELQKALLDLAEAQADTV
                     MPGFTHLQVAQPVSLGHHLMAYFEMFARDAQRFAEARRRVNRLPLGAAALAGTSFPID
                     RVMVAKELGFDGVCENSLDAVSDRDFAIEFCAHGALVMMHLSRFCEEIVLWTNPRFGF
                     "
     gene            870..1883
                     /locus_tag="VFY80_06500"
     CDS             870..1883
                     /locus_tag="VFY80_06500"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6007593.1"
                     /translation="MAGSINQNAYSSALPNFRNLGILLRILLTVNLAAIAGALVESKG
                     PHDFWLRLLDVSIVVQPLLMLSLLALVILNDVLRRLPYYLGAAAVLGIELVLATLLHL
                     YGKPLQPIEANWIERYWFLTFLMTLLLLLYFDLRGRALSPAISEARLQALQARIRPHF
                     LFNSINAVLSLIREDPKRAERALEDMADLFRVVMADNRELTPLAREVELCREYLALEQ
                     LRLGERLTVEWHIDNMPADARIPPLVLQPLLENAVYHGIEPRSAPGVISINIYLAREQ
                     VHAVLRNPYQHEGNHHAGNKMALANIRERLQLHFDAEAALATRVTDDAYQVHITMPYV
                     KGR"
     gene            1883..2650
                     /locus_tag="VFY80_06505"
     CDS             1883..2650
                     /locus_tag="VFY80_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6007594.1"
                     /translation="MSEPSTEPLRVVIVDDEAPARSRMRDLLADCAPHLALTIAGEAD
                     SGRAALALLSERPVDVVLLDIRMPEMDGIEVAQHLQKLESPPAVVFTTAYDAYAIRAF
                     EVHAIDYLLKPIRAARLREALARARAAARPKPETLRELQPTPRAFLSAQERGKIHLIP
                     IADVIYLKAELKYVTVRDAQREYLVEESLTRLEQEYSERFVRVHRNCLVARESIRGFE
                     RAAGESAEGSWLVLLEGCEEKIAVSRRQQHIVREFRK"
BASE COUNT          489 a          899 c          913 g          488 t
ORIGIN      
        1 atgaatccga agcgcgggtt cgtccacagc acgatctctt cgcaaaaacg cgacagatgc
       61 atcatgacta gggcgccgtg cgcgcagaat tcgatcgcga agtcgcggtc ggacaccgca
      121 tccagggagt tctcgcagac cccgtcgaat ccgagttcct tcgccaccat cacgcgatcg
      181 atcggaaagg aggtgcctgc cagcgctgct gcgccgagcg gcaggcgatt gacgcgtcgc
      241 cgggcttccg cgaagcgttg tgcgtcgcgt gcgaacatct cgaagtacgc catcaggtga
      301 tggccgagcg aaacaggctg tgcgacctgc aggtgcgtga atccgggcat caccgtgtct
      361 gcttgcgcct cggccaggtc gaggagtgcc ttctgcagct cccggatgag cgtcaggaca
      421 tggtcgatcg aagcccgcag atagagccga acatcggtcg cgacctggtc gttgcgcgag
      481 cgtgcggtgt gcaggcgctt tcctgcatcc ccgataaggt cggtgaggcg ccgctcgatg
      541 ttgaaatgca cgtcctcgta ctcgagcgac cacgggaacg tgccggcgcg cacttcgccg
      601 cggagctgct ccatgccctt ggtgatcgca gcgagatccg ccgccgaaag tatgccggcc
      661 gcacagagca tgcgcgcgtg ggcgagcgag ccgtcgatgt cgaattccgc gagccgctga
      721 tcgaacgaca ccgaagccgt gaagcgctgg acgagatccg tgatcggctc cctgaagcgc
      781 cccgaccagg gtttgctgcg gccggcggga ggcctgtcgt tggcggccat gatgcctgtt
      841 gtggggatcg gttctaaaat gggtgcagca tggccgggag tataaatcaa aacgcttaca
      901 gcagcgccct gccgaacttc cgcaatctcg gcatactgct gcgcatcctg ctcaccgtga
      961 acctcgcagc gattgcgggc gcgctggtgg aaagcaaggg gccgcacgat ttctggctgc
     1021 gccttctcga tgtgtcgatc gtggtgcagc cgctgctcat gctgagtctt ctcgcgctgg
     1081 tgatcctcaa cgatgtcctg cgccgtcttc cctattacct tggcgcggcc gcggtgctcg
     1141 gcatcgagct cgtgctcgcg acgctgctgc acctctacgg aaaaccgctc cagccgatcg
     1201 aagcgaactg gatcgagcgt tactggtttc tcacctttct catgacgctg ctcctgctgc
     1261 tctatttcga tttgcgcggc cgcgctctat cgcccgcgat atcggaggcg cgcctgcagg
     1321 cgcttcaggc gcgcatccgc ccgcacttcc tcttcaacag catcaacgcg gtgttgagcc
     1381 tgatccgcga ggatccgaag cgcgcggagc gcgcgctcga ggacatggcg gatctcttcc
     1441 gcgtcgtcat ggcggacaac cgcgagctga cgccgctcgc gcgcgaagtc gagctctgcc
     1501 gggagtatct cgcgctcgaa caattgcggc tcggggaacg gctcacggtg gagtggcaca
     1561 tcgacaacat gccggcggat gcgcgcatcc cgccgctcgt tctgcagccg ctgctcgaaa
     1621 acgcggtcta ccacggcatc gagccgcgca gcgcgccggg cgtcatcagc atcaacatct
     1681 atctcgcacg cgagcaggtg cacgcggtgt tgcgaaaccc gtatcagcat gaaggcaacc
     1741 atcacgccgg aaacaagatg gcgctcgcga acatccgcga acggcttcag ctgcacttcg
     1801 atgcggaggc ggccctcgcg acgcgtgtga ctgacgatgc ctaccaggtc catatcacca
     1861 tgccgtacgt gaaggggcga tgatgagcga gccttcgacc gagccgctgc gcgtcgtgat
     1921 cgtggatgac gaggcgcccg cgagaagccg catgcgcgac ctccttgccg attgtgcgcc
     1981 gcatcttgcg ctcacgatcg cgggcgaggc ggatagcggc cgcgcagcgc tcgcgctgct
     2041 gtccgagcgg ccagtggacg tggtcctgct cgacattcgc atgccggaaa tggacggcat
     2101 cgaagtggcg cagcatttgc agaagctcga gtcgccgccc gcggtcgtgt ttacgaccgc
     2161 gtatgacgcc tatgccatcc gcgcgttcga agtgcatgcc atcgactatc ttctgaagcc
     2221 cattcgcgcg gcgcggctgc gcgaggcgct cgcacgcgcg cgggcggccg cgcggccgaa
     2281 gcccgagacc ttgcgcgagc tgcagccgac gccgcgcgcg ttcctgagcg cgcaggagcg
     2341 cgggaaaatt catctgatac cgattgcgga cgtgatctac ctcaaggcgg agctcaagta
     2401 cgtcaccgtg cgcgacgcgc agcgcgagta tctcgtggag gagtcgctca cgcggctcga
     2461 gcaggagtac agcgagcgct tcgtgcgcgt gcaccgcaac tgcctcgtcg cgcgcgagtc
     2521 gatccgcggt ttcgagcgcg ccgccgggga gagtgcggaa ggcagctggc tcgtgctgct
     2581 cgaaggctgc gaggaaaaga tcgcggtgag ccgccgacag cagcatatcg ttcgcgagtt
     2641 ccggaagtga cccatccccg ggggcgcggc acggcccgcg tgctagaatc gccgtgcagt
     2701 acctcccgcc tcattccacg acggagcgcc gttgaacgtc cccgggtcga acggtggaac
     2761 tcgacgcatc gtgatcgcgt cgcgcgaaa
//
LOCUS       DASURG010000371         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_983769, whole genome shotgun sequence.
ACCESSION   DASURG010000371 DASURG010000000
VERSION     DASURG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983769"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /gene="plsY"
                     /locus_tag="VFY80_06510"
     CDS             complement(<1..481)
                     /gene="plsY"
                     /locus_tag="VFY80_06510"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX6007595.1"
                     /translation="MGIALFALVGYLIGSISFAVIVSRAFALPDPRTYGSGNPGATNV
                     LRTGRKAAAALTLLGDGAKGWLAVFLAQGFAQPETSALAMASAAVAVVLGHMYPVFHR
                     FRGGKGVATALGVLLGLNIYLALGSAATWIVIAAFFRLSSLAALVSALFAPFFTLILF
                     "
     gene            569..928
                     /locus_tag="VFY80_06515"
     CDS             569..928
                     /locus_tag="VFY80_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX6007596.1"
                     /translation="MDIIFIHDFRLDILIGIHEWERRVPQTIQFDLEIAIPPKVGPRT
                     DRIGETIDYSKVVKRIEETLRTQHFLLLERLSEHVAQLVLTEFGAPWVKVSVNKIGAL
                     RNVKRLGVTIERGRRSA"
     gene            complement(931..1830)
                     /gene="xerD"
                     /locus_tag="VFY80_06520"
     CDS             complement(931..1830)
                     /gene="xerD"
                     /locus_tag="VFY80_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX6007597.1"
                     /translation="MKAPESALIDEFCDALWLEDGLAPNTLTSYRRDLMKFGSWLAAK
                     QRRALLAATHADLLGFLAHEVQRRAKATTASRLLSSLKRFYRYALRQGKIRTDPTLNI
                     DAPKLPRPLPKSLTEQDVEALLAAPNVEEALGLRDRAMLETLYASGLRVSELVTLRLT
                     QVSQDMGVVRVTGKGSKERLVPLGEEALAWIRRYLREGRPALLRRRATDALFVTVRGE
                     AMTRQAFWYLIKRYALRAGLTKPLSPHTLRHAFATHLLNHGADLRVVQLLLGHSDIST
                     TQIYTHVARERLKQLHARHHPRG"
     gene            complement(1802..2284)
                     /locus_tag="VFY80_06525"
     CDS             complement(1802..2284)
                     /locus_tag="VFY80_06525"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6007598.1"
                     /translation="MLETYQARHPTPFAVLGIRTGSGMLKGIDYLPRGAATLSPLDRV
                     AEETCRQIERYLDNPEFPFDVPFEFDGTPFQCRVWREIARIPSGNTLTYLAVARRLRS
                     APRPVGGACGANRIPLVIPCHRVVASGGLGGFMNAKSGSPLEIKKWLLAHEGARIRAD
                     "
     gene            complement(2293..>2981)
                     /locus_tag="VFY80_06530"
     CDS             complement(2293..>2981)
                     /locus_tag="VFY80_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6007599.1"
                     /translation="ADVGARGLVEVWAFFGAERTPALLHEMKLLLTAFYTALIVWIVG
                     LAVPVTASYIICAVIAAPALIALKVPDFAAHMFIFYYAVLSEVSPPTALSPFAAAAIT
                     GGGPYRTTLQCWKYTAPAFLLPFMFVLDPAGIGLLLTGSFKNLGNADWGSIALVTVTA
                     AIGIAALAGGLQGWLFRRCNALERGLLIVAGLMLVYPRPLFDYVGCALVITVMLLQKL
                     RHSALRTASG"
BASE COUNT          589 a          950 c          992 g          450 t
ORIGIN      
        1 tgaagaggat cagcgtgaag aacggcgcaa acagggcgga aaccagcgcg gccagcgatg
       61 agaggcgaaa gaacgcggcg atcacgatcc acgtcgcggc gctgccgagc gcaagataga
      121 tgttgagccc gagcagcacg ccgagcgccg tggccacgcc cttgccgccc cggaaccggt
      181 ggaataccgg atacatgtga cccagcacca cggcgacggc ggcgcttgcc atggcgagcg
      241 cactcgtctc cggttgcgca aagccctgtg cgagaaacac cgcgagccaa cccttcgcgc
      301 catcaccgag gagcgtgagg gctgcggccg ctttccgtcc ggtacgcagc acgttcgtgg
      361 cccccggatt tcccgagccg taagtgcgcg gatcgggcaa cgcgaaggcc cgactgacga
      421 tgactgcaaa cgagatggag ccgataagat agccgacgag cgcgaaaagt gcgatgccca
      481 tgggatttca catagaatgt gcatccattc tatccgaaac gctcggcccg acgccgttac
      541 gcagtgggtc tcttgcgcgc gttccttcat ggacatcatc ttcatccacg acttcaggct
      601 tgacatcctg atcggcatcc acgagtggga acggcgcgtg ccgcagacga tccagttcga
      661 tctcgagatc gccatcccgc cgaaagtcgg gccgcgcacc gatcggatcg gcgagacgat
      721 cgactactcg aaggtggtga agcggatcga ggagaccctc cgcacgcagc actttctcct
      781 gctcgagcgc ctctcggagc acgtcgcaca gctcgtcttg accgaattcg gcgcgccgtg
      841 ggtcaaagta agcgtgaaca agatcggcgc gctgcgcaac gtcaagcggc tcggcgtaac
      901 gatcgagcgc ggcagacgga gcgcctgacg ctatccgcgc ggatggtggc gcgcgtgaag
      961 ctgcttcaat cgttcacgcg ccacgtgcgt gtagatctgg gtggtggaaa tgtccgaatg
     1021 tccgaggaga agctgcacca ccctcaaatc cgccccgtga ttgagcaggt gcgtcgcaaa
     1081 cgcgtgccgc agcgtgtgcg gtgagagggg cttcgtgagc cccgcgcgca aagcgtagcg
     1141 cttgatgaga taccagaacg cctgacgcgt catcgcctct ccacgcacag tgacgaagag
     1201 ggcatcggtg gcgcgacgcc ggagcaatgc aggccgccct tcccggagat agcggcgaat
     1261 ccacgcgagc gcttcctcgc cgagcggcac caggcgctcc ttggagccct tgccggttac
     1321 acgcacaaca cccatgtcct gactcacctg cgtgagccgg agcgtgacga gttcggaaac
     1381 acgcagcccg ctcgcataca gcgtctcgag catggcgcgg tcgcgaagcc ccagcgcctc
     1441 ctccacgttg ggcgcggcga gcagcgcttc cacgtcctgc tcggtgaggc tcttcggcag
     1501 cgggcggggc agcttggggg catcgatgtt cagcgtggga tctgtccgaa tcttgccctg
     1561 gcgcagggca taccggtaaa agcgcttgag ggaggacaac aggcgactcg cggtcgtcgc
     1621 cttggcgcgc cgctgcactt cgtgagcgag aaacccgagc agatccgcgt gcgtcgccgc
     1681 cagcagcgca cggcgctgct tcgccgcgag ccacgaaccg aacttcatga ggtcgcgccg
     1741 gtagctcgta agcgtgttcg gcgcgagccc gtcctcgagc cacagcgcgt cgcagaattc
     1801 gtcaatcagc gcggattcgg gcgccttcat gcgcgaggag ccatttcttg atctcgagcg
     1861 gcgagccgct tttcgcattc atgaatccgc cgaggccgcc gctcgccacc acgcgatggc
     1921 acggaatcac gagcggaatg cggttcgcgc cgcacgcgcc gcccaccggg cgcggtgccg
     1981 agcggagccg gcgtgctacg gcaaggtaag tcaacgtgtt gccgcttggg atgcgtgcga
     2041 tctcgcgcca gacgcggcac tggaacggag tgccgtcgaa ctcgaacggc acatcgaacg
     2101 ggaattccgg attatcgagg tagcgctcga tctggcggca ggtctcttcg gcaacgcggt
     2161 cgagcggcga cagcgtggcc gccccgcgtg gaagataatc gatgcctttc agcatgccgc
     2221 ttccggtccg gatgccgagc acggcaaacg gcgtgggatg gcgagcctga tatgtctcga
     2281 gcatcgcgcc catcacccgg aagcggtacg cagggcggaa tgccgcagct tctgcaacag
     2341 catgacggtg atcaccaggg cgcagccgac atagtcgaag agcgggcgcg gatagacgag
     2401 catgaggccc gccacgatga gcagcccccg ctcgagcgcg ttgcagcggc ggaacagcca
     2461 tccctgcagg ccgcccgcga gcgcggcgat gccgatcgcg gccgtaactg tcacgagcgc
     2521 aatcgaaccc cagtcggcgt tcccgaggtt cttgaacgag ccggtcagca atagaccgat
     2581 accggcggga tcgagcacga acatgaacgg caggaggaag gccggcgccg tgtatttcca
     2641 gcactgcagc gtggtgcggt acggcccgcc gcccgtgatc gccgcggcgg cgaagggcga
     2701 aagggcagtc ggcggcgaga cttccgacag cacggcgtag tagaagatga acatatgcgc
     2761 ggcgaagtcc ggaactttca gggcgatcag tgcgggggcg gcgatcacgg cgcagatgat
     2821 gtatgacgcc gtaaccggca ccgcgagccc tacgatccat acgatgaggg cggtgtagaa
     2881 cgcggtcagc aagagcttca tctcgtgcag cagcgcaggc gtgcgctcag cgccgaagaa
     2941 ggcccacact tcgacgagcc ctcgcgcacc gacgtccgca t
//
LOCUS       DASURG010000372         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1495951, whole genome shotgun sequence.
ACCESSION   DASURG010000372 DASURG010000000
VERSION     DASURG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495951"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFY80_06535"
     CDS             <1..532
                     /locus_tag="VFY80_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6007600.1"
                     /translation="CGCRVGLRPAFATVSFIHAVACAHSRIAMDLVLWRHADAESGIP
                     DAERRLTPEGRRQAEHIAGWLRPRLRQDCIVLVSPARRAQETARALADSFSVRAEVGT
                     TATPQSVLEVAGWPDREGSVVVVGHQPTLGQAAALALCGKIFDLDIEKGALWWLVGNP
                     GAPKPRLRLVIAPDML"
     gene            complement(529..1200)
                     /gene="rpiA"
                     /locus_tag="VFY80_06540"
     CDS             complement(529..1200)
                     /gene="rpiA"
                     /locus_tag="VFY80_06540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX6007601.1"
                     /translation="MNQDEMKKAAAAAAIDYVPEESVIGVGTGSTANFFIEALQRLKD
                     SLKGAVASSETTAQRLQALGIRVVDLNSVSDLPVYVDGADEVTRELAMIKGGGGALTR
                     EKIVAAVARKFVCIADESKLVQTLGKFPLPVEVIPMARAHVAREVEKLGGKPVWRQGF
                     VTDNGNAILDVHQLKIENPAELESTLNQIAGVVASGLFARRRADVLLLGGASGVQTLT
                     TRVCV"
     gene            1300..2814
                     /gene="ilvA"
                     /locus_tag="VFY80_06545"
     CDS             1300..2814
                     /gene="ilvA"
                     /locus_tag="VFY80_06545"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX6007602.1"
                     /translation="MKKNDYLERILKARVYDVAVETPLEPARSLSRRLGNRLLLKRED
                     MQPIFSFKLRGAYNKMAHLSPAALKRGVIAASAGNHAQGVALAAERLRCAATIVMPVT
                     TPPIKIDAVRARGAKVVLYGDTYDEACAQARTLQRRHGLTFVHPYDDPDVIAGQGTIG
                     MEILRQHAEPIHALFVAIGGGGLISGIGAYVKRLRPEIRVIGVEPEDADAMYRSLKAG
                     RRVKLDQVGLFADGVAVKQVGKETFRLCRQLVDEVILVDTDAMCAAIKDVFEDTRAIL
                     EPAGALAIAGAKAYVERSRIHDRTLIAVACGANMNFDRLRFVAEEAELGEAREAILAV
                     TIPEHPGSFKAFCSLVGPRNITEFNYRYADPREAHVFVGVQVQDRAETARLVRALRRR
                     GLKTLDFSDNELAKLHIRHLVGGHAATVKDEILYRFEFPERPGALMRFLTAMSRGWNI
                     SLFHYRNHGADYGRVLVGMQVPARDKRRFQAFLSEVGYPYRNESRNPAYKLFLG"
     gene            2846..>3109
                     /locus_tag="VFY80_06550"
     CDS             2846..>3109
                     /locus_tag="VFY80_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007603.1"
                     /translation="MIGRSRPFRFLIGAAFALACLSAAAGEEDDFLAAREAFRMGDAA
                     KLERHAERLRGYVLEPYVTYWRLRMRLEQADPGELRMFIERYAD"
BASE COUNT          555 a          993 c          995 g          566 t
ORIGIN      
        1 gtgcggatgt cgcgtcgggc tacgtcccgc cttcgcaacc gtaagcttca ttcacgcggt
       61 cgcatgcgcg catagccgga tcgcgatgga cctcgtcctc tggaggcacg ccgatgcgga
      121 aagcggcatc cccgatgccg agcgccgcct gacgcccgag ggacggcgcc aggccgagca
      181 catcgcggga tggctcaggc ctcggctgcg gcaggattgc atcgttctcg tgagcccggc
      241 gcggcgggcg caggaaacgg cgcgggcgct tgccgattcg ttttccgttc gtgctgaggt
      301 cggcacgacc gcgacacccc agtccgtgct cgaggtcgca ggctggccgg accgggaagg
      361 cagcgttgta gtcgtcggcc accaacccac tctgggtcag gcggcggcgc tcgcgctgtg
      421 cgggaaaatt ttcgatctcg acatcgagaa gggcgcactc tggtggctcg tcggaaatcc
      481 aggcgccccg aagcctcgcc ttcgtctcgt gatcgcgccc gatatgctct agacgcagac
      541 ccgcgttgtc agagtttgca cgccgcttgc gccccccaac agcagcacgt ccgcccgtcg
      601 ccgggcgaag agtccgctcg caaccacgcc ggcgatctgg ttgagcgtgc tttcgagttc
      661 tgccgggttt tcaatcttga gctggtgtac gtccagtatg gcgtttccgt tgtccgtgac
      721 gaagccttgc cgccataccg gcttgccgcc cagcttttcc acttcgcgcg cgacatgggc
      781 gcgcgccatc ggaatcactt ccaccggcag gggaaacttg ccgagcgtct gcacgagctt
      841 cgattcatcg gcgatgcaga cgaacttgcg ggcgaccgcc gccacgatct tttcccgcgt
      901 gagcgcacca ccgccgccct tgatcatcgc gagctcgcgc gtcacctcgt ccgcgccgtc
      961 gacgtagacc gggagatccg aaacgctgtt caggtccacg acccgaatgc cgagcgcctg
     1021 caagcgctgt gccgtcgttt ccgaactcgc tacggcaccc ttgaggctgt ctttgagacg
     1081 ttggagcgct tcgataaaga agttcgcagt ggagcccgtt cccaccccga tcacgctttc
     1141 ctcgggaacg taatcgatcg ctgcagcggc ggcggccttt ttcatctcgt cctggttcac
     1201 gaaagcatgg taaacgggaa tgcgcagccg gtaaaccgcg cggcgacgct tacctcgtgc
     1261 agtcattcct gctaccctta agcgcacttt gccgcaacga tgaagaagaa cgactacctc
     1321 gaacgcatcc tcaaggcccg ggtatacgac gtcgcagtcg agacgccgct cgagccggca
     1381 cgcagtcttt cccgccggct cggaaaccgc ctgctcctca agcgtgagga catgcaaccg
     1441 atcttttcgt tcaagctgcg cggcgcatac aacaagatgg cgcacctgtc gccggcggcg
     1501 ctcaagcgcg gggtaatcgc ggcttcggcc ggtaaccatg ctcaaggcgt agcgcttgcg
     1561 gctgagagac tgagatgcgc agccaccatc gtgatgccgg tgacgacgcc gccgatcaag
     1621 atcgatgcgg tacgcgcgcg tggcgcaaaa gtcgtcctgt atggcgacac gtatgacgaa
     1681 gcctgcgcgc aggctcgcac cctgcagcgc cggcacggcc tcaccttcgt gcacccttac
     1741 gacgacccgg atgtgatcgc gggccagggc acgatcggca tggaaatcct gcgccagcac
     1801 gcggagccga tacatgcgct ctttgtcgcg atcgggggtg gcggactgat ctcgggcatc
     1861 ggcgcttacg taaagcggct gcgtcccgag atccgtgtga tcggggtgga acccgaggac
     1921 gccgatgcaa tgtaccgatc gctcaaggcg ggtcgtcggg taaagctcga ccaggtcggc
     1981 cttttcgccg atggcgtcgc ggtgaagcag gtcgggaaag agacgtttcg cctctgccgg
     2041 caactcgtgg atgaggtgat cctcgtcgat accgatgcca tgtgtgcggc gatcaaggac
     2101 gtgttcgagg atacgcgggc aatcctggag cctgctggtg ccctggcgat cgcaggggcc
     2161 aaggcatacg tggagcgatc ccgcatccac gaccggacgc tcatcgcagt cgcttgcggc
     2221 gccaacatga acttcgaccg gctccgcttc gttgccgagg aggcggaact cggcgaggcg
     2281 cgcgaagcga ttctcgccgt cacgattccg gagcacccgg gaagcttcaa ggccttctgc
     2341 tcgctcgtgg gaccgaggaa catcaccgag ttcaattacc gctacgcgga tccgcgcgag
     2401 gcgcacgtgt tcgtcggggt gcaggtgcag gaccgcgccg aaaccgcgcg gttggtgcgc
     2461 gcgttgcgtc gccgcggact caagaccttg gacttcagcg acaacgagct cgccaagctc
     2521 cacatccgtc atctggtcgg gggacatgcg gcgacggtca aggatgagat cctctaccgc
     2581 ttcgagttcc cagagcggcc gggcgcgctc atgcgcttcc tcacggcgat gagccgcggc
     2641 tggaacatca gcctctttca ctaccgcaat cacggcgccg attacgggcg cgtgctggtc
     2701 ggcatgcagg ttcccgcgcg ggacaagagg agattccagg cattcctgtc cgaggtcggc
     2761 tatccgtatc gcaacgaaag ccgcaatccc gcctacaagc tgttccttgg ctaaaatacg
     2821 gctgacagtt actcggcaga aaacgatgat cggcaggtca cgaccattca gatttctcat
     2881 aggggctgcg tttgcgctcg cgtgcttgag cgcagcggcg ggcgaagagg acgattttct
     2941 ggcggcacgc gaggcgttcc ggatgggaga cgccgcaaag ctggagcgac atgccgagcg
     3001 cctcagaggc tatgtcctgg aaccgtatgt cacttactgg cgcttgagaa tgcggctcga
     3061 gcaggccgac cccggggaat tgcgcatgtt catcgagcgg tatgcggac
//
LOCUS       DASURG010000373         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_984060, whole genome shotgun sequence.
ACCESSION   DASURG010000373 DASURG010000000
VERSION     DASURG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984060"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /gene="rpoD"
                     /locus_tag="VFY80_06555"
     CDS             <1..1303
                     /gene="rpoD"
                     /locus_tag="VFY80_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX6007604.1"
                     /translation="EEDGEIEEELEAAEEEDEEGGAVQSADMLRLKEEALKRFAVIRS
                     YYNKMMKALAKNGPRSRPYLQARDAISAELMNIRFSAKQVEALCDSLRKLVEEVRSYE
                     RHIMELCVDKAKMPRAHFIKEFPGKEGNLKWVGSEVTARRPWGEALARFEPAIVEQQQ
                     KLLDLQARVGIPIKDLKEINRQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQ
                     FLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIE
                     TINKMNRISRQILQETGQEPDPALLAEKMEMPEEKIRKILKISKEPISMETPIGDDDD
                     SHLGDFIEDQSTMAPNDAAVYASLRGVTKDVLDTLTPREAKVLRMRFGIEMNTDHTLE
                     EVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDTES"
     gene            1515..2501
                     /locus_tag="VFY80_06560"
     CDS             1515..2501
                     /locus_tag="VFY80_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX6007605.1"
                     /translation="MALPHADRFFEIRFESIGGLGAHAAGQVLATAAVLRMGLNGANF
                     SSYGSEKKGSLVRSFVRLADADRLIRTSAPVETPDAVVVFHAALLHNPATFSGLRQDG
                     TFIYNAPAGLIPKELAALPRTARVIRVDALGIAMQEKSRANAVLLGTLCGAFSFLDSE
                     EILDAFSEEFAARHPEAVATNARAFRRGAVEFEVLSEVGEAEGDLPAARSTPAWGYET
                     QPLGGILPEPGNMAWNDLSASRTGLMPVLHAEKCIHCGVCDLVCPDFCLAWGEGEEGG
                     KYERMLKGIDYRYCKGCLRCVESCPSAALTTEKETPGLADRLRVPLFPELIG"
     gene            2513..>2902
                     /locus_tag="VFY80_06565"
     CDS             2513..>2902
                     /locus_tag="VFY80_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007606.1"
                     /translation="MTTHLAVAAHAADARMSGAVRQKLEFRSGNEAAALAARDIGFHL
                     MGYFPITPSTEVAESLAKMGAEGAHDIVMVAADGEHGAAGICYGAALGGGRVLNATSS
                     QGLLYALEQLPVQAGTRVPMVLNIAART"
BASE COUNT          579 a          904 c          955 g          464 t
ORIGIN      
        1 ggaagaggac ggcgagatcg aggaggagct cgaagcggcc gaagaagagg acgaggaagg
       61 cggcgcggta cagagcgccg acatgctcag gctcaaggaa gaagcgctga agcgcttcgc
      121 cgtcatccgc agctactaca acaaaatgat gaaggcgctc gccaagaacg gcccgcgcag
      181 ccgcccctat ctccaggcgc gcgatgccat ctccgcagag ctgatgaaca tccgtttctc
      241 ggcgaagcag gtggaagcac tgtgcgacag cctgcgcaag ctcgtcgagg aggtccgcag
      301 ctacgagcgc cacatcatgg agctgtgcgt ggacaaggcc aagatgccgc gcgcgcactt
      361 catcaaggaa ttccccggca aggaaggcaa tctcaagtgg gtcggctccg aagtcaccgc
      421 gcgccgtccg tggggcgagg cgctcgcgcg ctttgagccg gcgatcgtcg agcagcagca
      481 gaagcttctc gacctccagg cgcgggtcgg catcccgatc aaggatctga aggaaatcaa
      541 ccgccagatg tccaccggcg aggcgaaggc gcgccgggcg aagcgcgaaa tgacggaagc
      601 gaacctgcgg ctggtgatct cgattgcgaa gaaatatacc aaccgcgggc tgcaattcct
      661 ggatctgatc caggaaggga atatcggcct catgaaggcg gtggacaagt tcgaataccg
      721 ccgcggctac aaattctcga cctatgcgac gtggtggatt cgccaggcga tcacgcgctc
      781 gattgcggac caggcgcgca ccatccgcat tccggtgcac atgatcgaga ccatcaacaa
      841 gatgaatcgc atctcccgcc agatcctgca ggaaacgggc caggagccgg accccgcgct
      901 gctcgcggaa aagatggaga tgccggaaga gaagatccgc aagatcctga agatctcgaa
      961 ggagccgatt tcgatggaaa caccgatcgg cgacgacgac gattcgcacc tcggcgactt
     1021 catcgaggat cagtccacca tggcgcccaa cgacgcggcg gtctacgcca gcctgcgcgg
     1081 tgtgacgaag gacgtgctcg acacgctgac cccgcgcgaa gcgaaagtgc tccgcatgcg
     1141 cttcggcatc gagatgaaca ccgaccacac gctcgaagag gtcggcaagc agttcgacgt
     1201 gacgcgcgag cgcatccgcc agatcgaagc gaaggcgctg aggaaactgc gccatccctc
     1261 gcgctcggag cggctgcgca gcttcctcga caccgagagc tgatcgcagc aacttggtcc
     1321 taagcccgtc cacggggtcg ggcttcgttt tcctgcgcgt cctcaatgag gtagcgtccg
     1381 cggcacagcg ctgcaggcgg ggtgctgcgc agcgcgtttg atccacatca gtaccggcgc
     1441 aggttgcgag gcgataatcg caaccaatgc gacccgaaaa atcaatccaa gaaatcgctg
     1501 gcgcacagcg cccggtggcg ctgccccacg cagaccgttt ctttgaaatc cgtttcgagt
     1561 cgataggcgg gctgggtgcc cacgcggccg ggcaggtgct cgcgacggcg gctgtgctga
     1621 ggatgggact gaacggcgcg aacttctcct cgtatggttc ggagaagaag ggctcgctcg
     1681 tgcgctcgtt cgtgcgcctc gccgacgccg accggctgat ccgtacgagc gcgccggtgg
     1741 agacaccgga cgcggtcgtc gtctttcatg ctgcgctcct gcacaacccg gcaacctttt
     1801 cgggcttgag acaagacggc accttcatct acaacgcgcc ggccgggctc attccaaagg
     1861 aacttgcggc cctgccgcga accgcacgcg tcattcgtgt cgatgcactc ggcatcgcga
     1921 tgcaggaaaa atcgcgcgcc aacgcggtgc tgctcggcac gctgtgcggg gcgttttcct
     1981 ttctcgacag cgaagaaatc ctcgacgcgt tttccgagga gttcgctgcg cggcatcccg
     2041 aggcggtggc gaccaacgcg cgcgcttttc gtcgcggcgc ggttgaattc gaagtcctct
     2101 ccgaagtcgg tgaggcggag ggcgatctgc cggcggcgcg ctcgacgccg gcctggggtt
     2161 acgagacgca gcccctgggc ggcatcctgc ccgagccggg caacatggcg tggaacgatc
     2221 tttcggcttc gcgcaccggc ctcatgccgg tactccacgc ggaaaaatgc atccactgcg
     2281 gcgtatgcga tctggtctgc cccgatttct gcctcgcgtg gggcgaaggc gaggaaggcg
     2341 gcaagtacga gcggatgctg aagggcatcg actatcgcta ttgcaaggga tgcctgcgct
     2401 gcgtcgaatc ctgtccttcg gccgcgctta cgactgagaa ggaaacgccc gggctggcgg
     2461 acaggctgcg cgtgccgctc tttcccgaac tgatcggata gggggaagaa ccatgaccac
     2521 ccacctcgca gttgccgcac acgcggctga tgcgcgcatg tccggcgccg tgcgtcagaa
     2581 gctcgagttc agaagcggca acgaagcggc cgcgctcgct gcgcgtgaca tcggcttcca
     2641 cctcatgggt tattttccga tcacgccttc gaccgaggtc gcggagagcc tcgcgaaaat
     2701 gggcgccgag ggtgcgcacg acatcgtgat ggtggcggcc gacggcgagc acggcgcggc
     2761 ggggatctgc tacggcgctg cgctgggcgg cgggcgcgtg ctgaacgcaa cgagttcgca
     2821 gggattgctc tatgcgctcg agcagcttcc cgtgcaggcg ggcacccgcg taccgatggt
     2881 cctcaatatc gcggcgcgca cc
//
LOCUS       DASURG010000374         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_678053, whole genome shotgun sequence.
ACCESSION   DASURG010000374 DASURG010000000
VERSION     DASURG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678053"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFY80_06570"
     CDS             <1..305
                     /locus_tag="VFY80_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6007607.1"
                     /translation="VFSTPSMTQFVETTAQALAAPYLKPGQGQVGVSVSIRHLAPTPL
                     GKKVRAEAELTGIDRRRLSFKVRVFDDVEQVGEAEHDRFVIDVDKYIERLKKKIGG"
     gene            complement(309..1187)
                     /locus_tag="VFY80_06575"
     CDS             complement(309..1187)
                     /locus_tag="VFY80_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6007608.1"
                     /translation="MPYANSDGARLYYEEAGKGTPIVFVHEFADDLYSWEPQLRYFSR
                     RYRCIAYNARGYPPSDVPKPVSKYSQAIATDDIANVMRHLKIRKAHIIGCSMGGFATV
                     HFGLRYARMALSLTAIGVGYGSDPDKKAQFAKDTEIMARRFEELGTPEAIKPYQIGPA
                     RVQFQNKDPRGFAHFCAEFAKHSALGSANTLRGIQARRPTVYSLERGLSKLKVPLHMV
                     TGDEDNNCLEPGVFIKRVCPSAWLTVVPGTGHAVNLEEPDFFNRITAEFLAQVDSGRF
                     HLRDPRSLIKSTMAKR"
     gene            complement(1230..2111)
                     /locus_tag="VFY80_06580"
     CDS             complement(1230..2111)
                     /locus_tag="VFY80_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6007609.1"
                     /translation="MPHASSNGVRLYYEEAGKGTAIVFVHEFSGDLMSWDAQVRRFSR
                     RYRCIAFNARGYPPSDVPKSASKYSYRLAADDIANLMRQLGVENAHVIGCSMGAYTTL
                     QFGLRYPRRARSLTLVGVGAGSDLHGQAQFRRDTAAQAARYLALGTREVVKMYLNHPA
                     RITLKRKDPRGFADFYARVEAHSALGSANTLRGIQAKRPSIYGLDARLRRLAVPTHVV
                     CGDEDPSALAPSLFLKRICPAVQLSVVAGTGHAVNVEEPAYFNDITAAFLAQVDSGRW
                     RARDTRSFNPSTMAHAR"
     gene            complement(2157..>2410)
                     /locus_tag="VFY80_06585"
     CDS             complement(2157..>2410)
                     /locus_tag="VFY80_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6007610.1"
                     /translation="TIIGWMKVFGLALIAQVAGQSLIAYAMAHLPPRLSSVGLLLQPV
                     MAAIFAWLILGETLGALQFAGALLVLIGIRIAHQAESTK"
BASE COUNT          429 a          740 c          793 g          448 t
ORIGIN      
        1 aggtcttttc cacgccatcg atgacgcagt tcgtcgagac gacggcgcag gcgctcgccg
       61 cgccctatct caagcccggt caggggcagg tcggcgtatc ggtgagcatc cggcacctcg
      121 cgccgacgcc gctgggaaag aaggtgcgcg cggaagccga gttgactggc atcgatcggc
      181 gcaggctcag cttcaaggtg agggtgttcg atgacgtcga gcaggtcggc gaagcggaac
      241 acgatcgctt cgtcatcgat gtcgacaagt acatcgagcg cctgaagaag aagatcggcg
      301 gctgatcgct agcgctttgc catggtggac ttgataagcg agcgcgggtc gcgcaaatgg
      361 aagcgcccgc tatccacctg tgcgagaaat tcggccgtga tgcggttgaa aaagtccggt
      421 tcctccagat tcaccgcgtg tccggttccc ggcacgacgg tgagccacgc cgacggacat
      481 acgcgcttga tgaagacgcc gggctcgagg cagttgttgt cctcgtcgcc cgtcaccata
      541 tggagcggca ccttgagttt cgacaacccg cgttcgaggc tatagacggt cgggcgccgc
      601 gcctggatgc cgcgcagcgt gttggcggag ccgagcgcgg agtgcttggc gaactcggcg
      661 cagaagtgcg cgaagccgcg cgggtccttg ttctgaaact gcacgcgcgc gggaccgatc
      721 tggtagggct tgatcgcttc cggcgtgccc agttcctcga agcggcgcgc catgatctcg
      781 gtgtctttcg caaactgcgc tttcttgtcg gggtcggagc cgtaacctac gccgattgcc
      841 gtgagtgaaa gcgccatccg cgcgtagcgc agtccgaagt ggacggtcgc gaatccgccc
      901 atcgagcagc cgatgatgtg cgccttgcgg atcttcagat gccgcatcac gtttgctatg
      961 tcgtcggttg cgatcgcctg ggagtacttg gagaccggct tcggcacgtc ggacggcgga
     1021 tagccgcgtg cgttgtaggc gatgcagcgg tagcgccggc tgaaatagcg aagctgcggc
     1081 tcccagctgt agagatcgtc ggcgaactcg tgcacgaaga cgatcggtgt tcccttgccc
     1141 gcctcttcat aatagagccg cgctccatcg gaatttgcgt agggcattgc agacctcctc
     1201 tcgcgtaaaa aagatcgacg tggggcgcct tatcgcgcgt gcgccatcgt cgaaggattg
     1261 aacgagcgcg tatcgcgcgc gcgccagcgg ccggaatcga cctgcgccag aaacgcggcc
     1321 gtgatgtcgt tgaagtatgc cggctcctcg acgttcactg cgtgaccggt tccggcaacc
     1381 acgctcagct gtaccgcggg gcatatgcgc ttcaaaaaga ggctgggcgc gagcgcgctc
     1441 gggtcttcgt cgccgcacac gacgtgcgtc gggacagcca ggcgccgaag ccgcgcgtcg
     1501 agaccataaa tgctgggccg tttcgcctga atgccgcgca gcgtgttggc gctgccgagc
     1561 gccgaatgcg cttccacccg cgcgtagaag tcggcgaatc cgcgcggatc cttgcgtttt
     1621 aaagtgatgc gcgccgggtg gttgagatac atcttgacga cttcacgcgt tccgagcgcg
     1681 agatagcgtg ccgcctgtgc cgcggtgtcg cgccggaact gtgcctgccc gtggagatcc
     1741 gatcccgcgc caacgccgac cagcgtgagc gatcgcgcac ggcgcggata acgcagtccg
     1801 aactgcagcg tcgtatatgc ccccatcgag cacccgatca cgtgcgcgtt ttcgacgccc
     1861 agttgccgca tgagattcgc gatgtcgtcc gcggcgaggc ggtatgagta ctttgacgcc
     1921 gacttcggca cgtcggaagg cggataaccg cgcgcattga atgcgatgca gcggtagcgg
     1981 cggctaaagc gccgcacctg cgcgtcccag ctcatgaggt cgcccgagaa ctcgtgcacg
     2041 aagacgatcg ccgtcccctt tccggcttct tcgtagtaga gccggactcc atttgaactt
     2101 gcatgcggca tgcgctttgt tcccgcttgt tgtggatgct gcggcgctac tttagcctat
     2161 ttcgtgctct cggcctgatg cgcgatgcgg atgccgatca gcacgagcag cgcgcccgca
     2221 aactgcagcg cgccgagcgt ttcgccgagg atgagccatg cgaagatcgc cgccatgacc
     2281 ggctgcagca gcaatccaac cgacgagagt cgcggcggca gatgcgccat ggcgtacgcg
     2341 atgagactct gccccgccac ctgcgcgatg agcgcgaggc cgaagacctt catccagcct
     2401 atgatggtcg
//
LOCUS       DASURG010000375         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1496300, whole genome shotgun sequence.
ACCESSION   DASURG010000375 DASURG010000000
VERSION     DASURG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496300"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="mobB"
                     /locus_tag="VFY80_06590"
     CDS             complement(<1..287)
                     /gene="mobB"
                     /locus_tag="VFY80_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX6007611.1"
                     /translation="MKIFGFAGYSGSGKTTLIEKLIPVFTGRGLAVSLIKHAHHTFDV
                     DQPGKDSWRHRHAGCSEVLVTSSRRWVLMHELRGAAEPSLAEHVKRMSACDV"
     gene            complement(364..1170)
                     /locus_tag="VFY80_06595"
     CDS             complement(364..1170)
                     /locus_tag="VFY80_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6007612.1"
                     /translation="MLAALLVAAPAPSVFAQNFITVASTTSTEQSGLFKHILPAFEKK
                     TGIQVRVVALGTGQALDMARRGDADVVFVHAKAAEEKFVAEGYGVKRLPVMYNDFVLV
                     GPKADPAKVRGGKDILQALRKIRAAKAPFVSRGDRSGTHMAEIALWKEAGIDVAAKKR
                     AWYRDTGQGMGPALNSASSMNAYILADRGTWIAFKNRGSLAILVEGDKRLFNQYGVIL
                     VNPAKHKHVKKEMGQAFIDWIVSTEGQKAIADYRIGGEQLFFPNAGEGDA"
     gene            complement(1194..1892)
                     /locus_tag="VFY80_06600"
     CDS             complement(1194..1892)
                     /locus_tag="VFY80_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6007613.1"
                     /translation="MLPLRLRDVSFAVNGRTLIDRVSFEVAAGPRTIILGANGAGKSV
                     LMRLCHGLLKPTAGEISWQSNGGRPGSQSMVFQRPVMLRRSALANVIYGLRLAGFSAR
                     ESALRALDVLEAVGLESQAEHPARILSGGEQQRLALARAWAPGPEVLFLDEPTANLDP
                     GSTRDIESIIGAIHATGTKIIMTTHNLGQARRLGDEIFFLNHGRLVERAPVDNFFKHP
                     ATAEAAAFIRGEMP"
     gene            complement(1895..>2467)
                     /locus_tag="VFY80_06605"
     CDS             complement(1895..>2467)
                     /locus_tag="VFY80_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6007614.1"
                     /translation="LPLGALLAVARFRGRQAIIVLLNALMGLPPVVVGLVVYLLLSRA
                     GPLGAFGILFTPAAMVIAQSILITPIIAALSRQTIEDAWNEYEEQLRSLGAGRFRASL
                     TLLRDTRYSLVTILLAGFGRAAAEVGAVMIVGGNIDGVTRVMTTAIALETSKGDLPLA
                     LGLGIVLVTLVVMLNAAASVIREAALRRYG"
BASE COUNT          467 a          801 c          765 g          434 t
ORIGIN      
        1 acatcgcagg ccgacatgcg ctttacgtgc tcggcaagcg acggctcggc tgcgccgcgc
       61 aactcgtgca tcagcaccca gcgtcgtgat gaggtcacca gcacttcgct gcatcctgca
      121 tgacgatggc gccacgaatc cttgcctggc tgatcgacgt cgaaggtgtg atgcgcatgc
      181 ttgatgagcg aaacggcaag cccgcgcccg gtgaagaccg gaatgagctt ctcgatcaac
      241 gtcgtcttgc cgctgccgga atagccggcg aaaccgaaga tcttcatgcc tcgaggcctg
      301 caaaacgcgc gagaaagcgc cgaatgatac tttgcccgaa tagcagggtc gagtcgcgca
      361 tgctcatgcg tccccttcgc cggcgttggg aaagaagagt tgctcgccgc caatcctgta
      421 gtccgcgatc gccttctgcc cctcggtaga gacgatccag tcgatgaagg cttgccccat
      481 ctcctttttg acgtgcttgt gttttgcggg attgaccagg atcacgccat actggttgaa
      541 aagccgcttg tcgccttcga ccagaatcgc aagactgccg cggttcttga acgcgatcca
      601 cgttccgcgg tcggcgagga tataggcgtt catcgaggac gcgctgttga gcgcggggcc
      661 catgccttgc cccgtatcgc gataccaagc gcgttttttc gcggcgacat cgatgcccgc
      721 ctccttccac aacgcaatct cggccatgtg cgtgccgctg cggtcgccgc gcgagacgaa
      781 gggagccttc gcagccctga tcttgcgcag cgcctggaga atgtctttgc ccccgcgcac
      841 cttggcgggg tcggccttcg gtccgacgag gacgaagtcg ttgtacatga ccggcagccg
      901 cttgacgcca tagccctcgg caacgaattt ttcttcggcg gctttcgcgt gcacgaagac
      961 gacgtccgca tcgccgcgcc gcgccatgtc gagcgcctga ccggtgccga gcgccactac
     1021 tcgcacctgt atgcccgttt tcttctcgaa tgcgggcagg atgtgcttga aaagccccga
     1081 ctgctccgtg gacgtcgtcg aagccaccgt gatgaaattc tgcgcgaaca ccgatggcgc
     1141 aggcgctgcg acgagcaatg cagccaacat cgtcatgaaa ccgcttctcg gcgtcatggc
     1201 atttctcctc ttatgaatgc agcggcctcc gcggttgcag ggtgcttgaa gaaattgtcc
     1261 accggcgcgc gctcgacgag tcggccatgg ttcaagaaaa agatttcgtc gcccaatcgc
     1321 cgcgcctggc ccaggttgtg ggtggtcatg atgattttgg tgccggtcgc gtgtatcgcc
     1381 ccgatgatcg attcgatgtc gcgcgtcgaa ccggggtcca ggtttgcggt cggctcgtcc
     1441 agaaaaagca cttcgggccc cggagcccag gcgcgcgcga gcgcgagacg ctgctgctcg
     1501 ccacccgata ggatgcgcgc cggatgctca gcctggcttt cgaggccgac ggcttcaaga
     1561 acgtcgagcg cgcggagcgc agactcgcgt gcggaaaagc cggcgagccg cagcccatag
     1621 ataacgttcg ccagcgccga gcgccgcagc atgacgggcc tctggaacac catcgactgg
     1681 cttccaggac gcccgccatt gctttgccag gagatctccc cggcagtggg cttcagcaac
     1741 ccatggcaca ggcgcatcag cacgcttttc cccgcgccgt tagcgcccag gatgatcgtg
     1801 cgcggccccg ctgcgacctc aaacgacacg cgatcgatga gcgtccggcc gttgacggca
     1861 aacgatacgt cacgcaatcg caacggcagc atcgtcaacc gtagcggcgc agcgccgcct
     1921 cccggattac gctcgccgcg gcattgagca tgacgaccag cgtgacgaga acgatgccga
     1981 ggccgagcgc gagcggcaag tcgcctttgc tcgtttcgag cgcgatggcc gtcgtcatca
     2041 cacgcgtcac gccatcgata ttgccgccca cgatcatcac tgcaccgacc tcggccgcgg
     2101 cccgaccgaa accggccagc aggatcgtta ccagggaata gcgggtatcc cgcagcaggg
     2161 tgagcgaggc ccggaaacga cccgcgccga gcgagcgcag ttgctcctcg tattcgttcc
     2221 acgcgtcctc gatggtctgg cgcgaaaggg cggcgatgat cggtgtgatg aggatcgatt
     2281 gcgcgatcac catcgcggca ggggtaaaca gtatgccgaa cgcgcccagc ggcccagcac
     2341 gcgacaggag cagatagacg acgagcccga ccaccaccgg gggcagaccc atcagcgcgt
     2401 tgaggagcac gatgatcgcc tggcgtccgc gaaagcgggc gacagcgagc agcgcaccga
     2461 gcggcag
//
LOCUS       DASURG010000376         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_167300, whole genome shotgun sequence.
ACCESSION   DASURG010000376 DASURG010000000
VERSION     DASURG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167300"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1525
                     /locus_tag="VFY80_06610"
     CDS             293..1525
                     /locus_tag="VFY80_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007615.1"
                     /translation="MSSAKGIREVAYVDTRQGGAVRREPVHARGFEPLAWHDCAGGGQ
                     VVVQGNIAYVGNMRNPHGTLIIDVADPRHPKPLATIDMPPGTHSHKVRVAGDIMLTNR
                     EVLGERVRRNEIPPEDFQGGLGIYDVANPAKPRLITHWKTTDKPGPSYARGVHRFDFD
                     GRYAYISPTWDGYIGNIVMILDLKDPARPEEVGRWWMPGQWIAGGEQPSWKGAAHKCH
                     HPLRFGNRLYTSYWHGGFVILDIDDMTKPRLVSHLDWSPPFPWPTHTCLRMPFKIDNR
                     DFMVVSDEDVVRLEGCPPYPSAFLWVVDITDEAHPMPVATFQLEGIPPAEQPHMTGCH
                     QPCEKVTGTEIPVAWFAHGLRIVDISRPHAPKEVAHFLPDPPPGSSRVQSNDVTVDDR
                     GLIYLLDRVRGLHILERT"
     gene            1540..>2232
                     /locus_tag="VFY80_06615"
     CDS             1540..>2232
                     /locus_tag="VFY80_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX6007616.1"
                     /translation="MATGVFDDALIRHLWSTEELRAIFSDRNRVQKWYDYEAALALEQ
                     AALGIIPDGAAREIAAHARVENVDIEAIAEGIRRIKHPLVPALKALQALCKPEHGEYV
                     HFGPTTQDVLDTATMLQIRESHAIYVRDMKAIGRALYRLAEAHRATPMVGRSHGVQAL
                     PITFGHKCAVWLSEMGRHHERMRQLEPRMFVGGMVGAVGTQASYGPLAAELEQGLMRR
                     LGLGVADVNWQPA"
BASE COUNT          415 a          719 c          733 g          365 t
ORIGIN      
        1 agctcccgca ccaacgcgaa gggtccgcga tgcagaaata cgccgcccag cccgagtgcg
       61 ctgccattcg cgatgcgaga agcaagctcg gagagcgtgc agactttgga gtgcggaacg
      121 ctgttcacgg tagtgctcca catgcatggg tccagtcgat catagcaccg ggcgcgcgac
      181 gcctcctgcg ttccgagccg atgccgtttg ctgataaact cgccgcttcc tttgttcaag
      241 aatactgcga cgcgcaccgc gcgatttctc tttacctcgg gggcacacca caatgagcag
      301 cgccaaaggc ataagggaag tcgcatatgt tgacacccgg cagggcggag cagtgcgccg
      361 ggagccggtc cacgctcgcg gtttcgagcc gctcgcgtgg cacgactgcg cgggcggcgg
      421 acaggtcgtg gtgcagggga acatcgcgta tgtcggcaac atgcgcaacc cgcacggcac
      481 gctcatcatc gatgtcgcgg atccgcggca ccccaagccg ctcgcgacga tcgacatgcc
      541 gccggggacg cattcgcaca aggttcgcgt ggcgggcgac atcatgctga ccaatcgcga
      601 ggtgctgggc gagcgcgtgc ggcgcaatga gattccaccg gaggatttcc agggagggct
      661 cggcatctat gacgtggcga atccggcgaa gccccggctc atcacgcact ggaagaccac
      721 cgacaagccg gggccgagct acgcgcgcgg cgtgcaccgc ttcgatttcg atggccgcta
      781 cgcttacatc tcgcccacgt gggacggcta catcggcaac atcgtgatga tcctcgatct
      841 taaggacccc gcgcggccgg aagaagtggg gcgctggtgg atgccggggc aatggatcgc
      901 aggcggcgag cagccgtcat ggaagggcgc ggcgcacaag tgtcatcacc cgctgcgctt
      961 cggcaaccgg ctctacacga gctattggca cggcggcttc gtcatcctcg atatcgacga
     1021 catgacgaag cccaggctcg tatcccatct cgactggagc cccccgtttc cgtggcccac
     1081 gcacacttgc cttcgcatgc cgttcaagat cgacaatcgc gatttcatgg tggtatccga
     1141 cgaagacgtc gtccgcctcg agggctgccc gccgtatcct tccgcatttc tgtgggtggt
     1201 ggacatcacc gacgaggcgc acccgatgcc ggtcgcgacc tttcagctcg aaggcattcc
     1261 acccgcggag cagccgcata tgacggggtg ccaccagccg tgcgaaaaag tgacgggcac
     1321 cgaaatcccg gtcgcgtggt ttgcgcacgg actgcgcatc gtggacatct ctcggccgca
     1381 tgcgccgaag gaggtcgccc atttcctgcc cgatccgccg cccggctcct cgcgtgtgca
     1441 gagcaatgat gtcacggtgg acgaccgcgg gctcatctac ctgctcgacc gcgtgcgcgg
     1501 cctgcacatt ctcgagcgga cgtgaaggag ggcgcgccca tggcgaccgg ggtattcgac
     1561 gacgcgctca tccggcactt gtggagcacg gaagagctgc gcgccatatt cagcgaccgt
     1621 aatcgcgtcc agaagtggta cgactacgag gcggcgcttg cgctggagca agcggcgctc
     1681 ggcatcatcc cagacggggc ggcgcgcgaa atcgcggccc acgcgcgcgt ggagaatgtc
     1741 gatatcgaag cgatcgccga agggatccgc cgcatcaagc atcccctggt gcccgcgctc
     1801 aaagccttgc aggcgctgtg caagccggag cacggagagt acgtccattt cggtcccacg
     1861 acacaggacg tcctcgatac cgcgacgatg ctgcagatca gggagtcgca tgcgatctat
     1921 gtgcgcgaca tgaaagcgat cggccgcgcg ctctatcgtc tggcggaggc gcaccgcgca
     1981 acgccaatgg tgggccgctc acacggcgtg caggcgctgc cgatcacctt cgggcacaag
     2041 tgcgcggtat ggctatccga aatggggcgc catcacgagc ggatgcgcca gctcgagccg
     2101 cgcatgttcg tgggcggcat ggtcggtgcg gtgggcacgc aggcgtccta tggtccgctg
     2161 gcagccgaac tggagcaggg tctcatgagg cgactcggcc tcggggtagc cgacgtcaac
     2221 tggcaacccg cg
//
LOCUS       DASURG010000377         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1188800, whole genome shotgun sequence.
ACCESSION   DASURG010000377 DASURG010000000
VERSION     DASURG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188800"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFY80_06620"
     CDS             <1..597
                     /locus_tag="VFY80_06620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6007617.1"
                     /translation="LVSTWIPALDGVEGKLRSGARVADVGCGHGASTLIMAQAYPESE
                     ITGFDYHEPSIRRAREAAAQAGVSDRVRFEVAAAKSFPGSDYDFVAVFDCLHDMGDPV
                     GAAAHVKQSLRSDGTWMIVEPYANDALERNINPVGRVFYSASTFICTPASRAQEVGMC
                     LGAQAGEQRIRDVVTNGGFTRFRRAAETPFNLVYEARP"
     gene            627..821
                     /locus_tag="VFY80_06625"
     CDS             627..821
                     /locus_tag="VFY80_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX6007618.1"
                     /translation="MARKYIDCREYPSEMNCTLAIAADSEQELLEAAVQHAVAVHGHH
                     DTPEFRRQLTSLFKDGAPPA"
     gene            848..1969
                     /locus_tag="VFY80_06630"
     CDS             848..1969
                     /locus_tag="VFY80_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6007619.1"
                     /translation="MRFTQQDFEGALDFVRDLYDQRGLDDFADRTMASLARIASAERV
                     IYGDFDIERQSARLAMRPAIVKEQDGTVDGVVDGGLSGLERNFGQHPLFRYFLQTGDG
                     RPQRATQVMTRSQHHHYSENDSFAKQLGARYQIGMFFAAAPAIVTTILLTRRERDFTE
                     RERALVDRLYPHLVQAFRNTASLNRLCRDVDELVEMLEGPTSSVVVLGGTGGVKRWSE
                     QAKKWVAQYCRTPFPAEADRLPDCFAEWYQRQLALAAHETLSPTPREPLIVDKNGRQL
                     TVQFVPDHFRDEHLLLLNEKRRDSSWTALGASGLTPRESEVLAWVAKGKTNAEVGAIL
                     QMSGRTVQKHLEHIYAKLGVGSRTTATVRALKLLGIEND"
BASE COUNT          412 a          728 c          714 g          366 t
ORIGIN      
        1 ctggtgagca cctggattcc ggcgctggac ggggtcgaag ggaagctcag atccggcgcg
       61 cgcgtcgcgg atgtgggctg cggccatggc gcatccaccc tcatcatggc acaggcgtat
      121 cccgaatccg aaatcaccgg cttcgattac cacgagcctt ccatccgacg cgcgcgcgaa
      181 gcggcggcgc aagccggcgt ctcggaccgc gtgcgtttcg aagtggcagc cgccaaaagc
      241 ttcccaggca gcgactacga cttcgtggcc gtgttcgatt gcctgcacga catgggcgac
      301 ccggtcggcg cggccgcgca cgtgaagcag agcctccgat ccgacggcac ctggatgatc
      361 gtcgagccat acgcgaacga tgccctggag cggaacatca atccggtggg gcgcgtcttc
      421 tattccgcct cgactttcat ctgcacgccg gcttcgcgtg cgcaggaagt ggggatgtgc
      481 ctcggcgcac aggccggcga acagcgcatc cgcgacgtcg ttacaaatgg cggcttcacc
      541 cgcttccgcc gtgcggcgga gacgccgttc aacctggttt acgaagcacg gccctgaagc
      601 gtattttcac ggagaggagg ttcgtcatgg cgcgcaaata catcgattgc cgtgagtatc
      661 cgagcgaaat gaactgcacg ctcgccattg cggccgacag cgagcaggaa ctgctggagg
      721 cggcggtgca acacgcggtc gcggttcacg ggcaccacga tacgccggaa tttcgccggc
      781 agttgacgag tctcttcaag gacggcgcgc cgcccgccta gagcggtgcg cgcgggagga
      841 ggccgtcatg cgcttcacgc agcaggattt cgaaggcgcg ctcgatttcg tgcgcgacct
      901 ctacgaccag cgcgggctcg acgacttcgc cgaccgcacg atggcttcgc tcgcccgcat
      961 cgcgagcgcg gagcgcgtga tctacggcga cttcgacatc gagcgccaga gcgcgcggct
     1021 cgccatgcgg ccggcgatcg tcaaggaaca ggacggcacc gtggacggcg tcgtggacgg
     1081 cggcttgagc gggctcgagc gcaacttcgg acagcatccg ctctttcggt attttctcca
     1141 gaccggagac ggcaggccgc agagagccac acaagtcatg acgcgctcgc agcaccacca
     1201 ctacagcgaa aacgactcgt tcgcgaagca gctcggcgcc cgctaccaga tcggcatgtt
     1261 cttcgccgcg gctcccgcga tcgtgactac gattctcctc acgcggcgcg agcgcgattt
     1321 taccgagcgc gagcgggccc tcgtggaccg gctctatccg catctcgtcc aggcctttcg
     1381 caataccgct tcattgaacc ggctctgccg cgatgtggac gagctggtcg agatgctgga
     1441 aggcccgacg agcagcgtgg tcgtgctggg aggcaccggg ggcgtgaagc gctggagcga
     1501 acaggcgaag aaatgggttg cgcaatactg ccgcacgccg ttccccgcgg aagccgaccg
     1561 gctgcccgac tgcttcgccg agtggtatca gcgccagctc gcgctcgccg cgcacgaaac
     1621 gctcagcccg acgccgcgcg aaccgctgat cgtggacaag aatggcaggc agctcaccgt
     1681 gcaattcgta ccggaccatt tccgcgacga gcacctgctg ctgctcaacg agaaacgccg
     1741 cgactcgtca tggactgcac tcggcgcgag cggcctcacg cctcgcgaat ccgaagtgct
     1801 cgcctgggtg gccaaaggca agaccaatgc ggaagtcggg gcgatcctgc agatgagcgg
     1861 ccgcacggtg cagaagcacc tcgaacacat ctacgccaag ctgggcgtcg gatcgaggac
     1921 caccgcgacg gtgcgcgcgc tgaagctcct cggcatcgag aacgactgat ttattgtcgt
     1981 tcccctggat tccagctttc gccggaatga cgaacatcgc tcgtcattcc cgtccttctc
     2041 catgtagtgc gtcgtagagc agtcgtttca accaccgcgc gctgctgctg tgcactgcgc
     2101 gccgtatggg tctagccggc gttgcgccgc acgaagcgct cgagcgaaat agtatagcgt
     2161 ccggtgcgct ccgaatagcg gacgcagtag gccgtcggat agaaaccgtc cactgcccac
//
LOCUS       DASURG010000378         4181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_167768, whole genome shotgun sequence.
ACCESSION   DASURG010000378 DASURG010000000
VERSION     DASURG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167768"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFY80_06635"
     CDS             <1..324
                     /locus_tag="VFY80_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6007620.1"
                     /translation="AARRITCNGHDIELGDIEFRMLHFFMTHPERVYSRAQLLDEVWG
                     DRVFVEERTVDVHIRRLRQTFKPTGHEELLETVRGVGYRFRRALPDGPAWPRPASADE
                     LLEAR"
     gene            513..1289
                     /gene="epsA"
                     /locus_tag="VFY80_06640"
     CDS             513..1289
                     /gene="epsA"
                     /locus_tag="VFY80_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HEX6007621.1"
                     /translation="MTDSASSRSDDLQAESLLLNLDASLRVYARHEFFSWTQGALQSL
                     LEHELLICAMPSGEPGSFYVDSFSTTLLEPARVSELFRQDVTLVPQLIKAWEENHCEP
                     VAFDEATVSAATGSALGRELARVGAHSILAHGTYDSSGELASFFTFGCVPGSAGSRER
                     HVVGLVVPFLHVAWMRTRINWPGDGTVVKPAVESVLTARETEILQWIYHGKSNIEIGM
                     ILNISALTVKNHVQKILRKLNVLNRTQAIGKALALRILNP"
     gene            complement(1300..3126)
                     /gene="glmS"
                     /locus_tag="VFY80_06645"
     CDS             complement(1300..3126)
                     /gene="glmS"
                     /locus_tag="VFY80_06645"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6007622.1"
                     /translation="MCGIVGAISKRNVVPILLEGLRCLEYRGYDSAGVAVINGSLQRL
                     RSTGRVAALATLAQAHGLRGTIGIAHTRWATHGMPSERNAHPHVSGGISVVHNGIIEN
                     HEAMRHALAAQGYEFVSETDTEVIAHLVHSHVCKGASLFEAVRASTADLVGAYAIAVA
                     FEADPTRLVVARMGAPLILGVGDSEFFAASDAAALIQVTRNVVYLEEGDCAEITLKGV
                     RIVDGKGAACQRPVRVSELTSESIELAPYRHHMQKEIFEQPMAVANTLEMVTNARSIS
                     PGVFGASAEGILREADALLILACGTSYHAGMVARYWLESVAGIPCSVEIASEYRYRES
                     VPNPRGLVVAISQSGETADTLAAVHHARKLGYRHCLAICNVPESALVRAADLRFLTRA
                     GPEIGVASTKAFTTQLAVLVLLTLTLAKLRGRLSGDQEAALLASLRHLPSALSNVLGV
                     EAAIEAWGRKFARRQHALFLGRGIHYPIAMEGALKLKEISYIHAEAYAAGELKHGPLA
                     LVDREMPVIAIAPKDALLEKLQSNLQEVRARGGELHVISDPDTRIHEGEGMHVIRMPD
                     HAGLLSPILHTIPLQLLAYYAALEKGTDIDKPRNLAKSVTVE"
     gene            complement(3219..3500)
                     /locus_tag="VFY80_06650"
     CDS             complement(3219..3500)
                     /locus_tag="VFY80_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007623.1"
                     /translation="MMNREAMIRKLVSYSVEAALREPQSYWLQELFEKGFAGYRRFSD
                     SRLRRELELRGLDETEEPDVEDDAPFDDVDIDDTSESFAPYVVRGKEYD"
     gene            3802..4062
                     /locus_tag="VFY80_06655"
     CDS             3802..4062
                     /locus_tag="VFY80_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6007624.1"
                     /translation="MTAEPVLTEREKEILHLIYQGKSNMEIAAALSINTLTVKDHVRK
                     ILRKLKVHNRTQAVGRAVALRIVTLPEAPQPPSSKRPSTLHH"
BASE COUNT          759 a         1299 c         1342 g          781 t
ORIGIN      
        1 gcagcgcgcc gtatcacctg caacggacac gacatcgagc tcggagacat cgagttccgg
       61 atgctccatt tcttcatgac ccaccccgag cgtgtctaca gccgcgcgca actgctggat
      121 gaggtgtggg gcgatcgcgt cttcgtcgag gagcgcacgg tggacgtgca catccgccgg
      181 ctgcgccaga cgttcaagcc caccggacac gaggagctgc tggagacggt gcgcggggtg
      241 ggatatcgat tccgtcgcgc cctgccggac gggccggcgt ggccgcgtcc cgcatccgcg
      301 gatgaactgc tggaagcccg atagaggcgc gagctctgtc gcatgcgggc gacgaatgcg
      361 ccggtcggca catacgccgc ttctcctgcg ttcacgaggt cgtgccacgt tatgttgact
      421 tgtctttcca agaggtgccg tgccccatgc gagcagacga gcgtttgacc ctacaagccg
      481 atcccctgct gtccgtctta ccgcaggcgg tgatgaccga cagtgcgtcg tcccgcagcg
      541 acgacctgca ggccgagtcc ctgctgctca atctcgatgc atccctacga gtgtatgcgc
      601 gccacgaatt cttcagctgg acacaaggcg cgctgcagag cctgctcgag cacgagctgc
      661 tcatctgcgc gatgcccagc ggcgagccgg gatccttcta cgtggacagt ttctcgacca
      721 cgctgctaga gcccgcgcgc gtgagcgagc tgttccgaca ggatgtgacc ctggtgccgc
      781 agctcatcaa ggcttgggaa gagaaccact gtgagccggt tgctttcgac gaagcgacgg
      841 tcagcgcggc gacaggcagc gctctcgggc gcgagctcgc ccgagtcggc gcgcacagca
      901 tcctggcgca tggtacgtac gacagcagcg gtgaactcgc gagcttcttc actttcggct
      961 gcgtgcccgg gtcggccgga agccgggagc gccacgtggt gggcctggtt gtgcctttcc
     1021 tgcacgtcgc atggatgcgc acccggatca actggcccgg agacggcacg gtggtcaagc
     1081 ctgcagtcga gagcgtgctg acggcgcgtg aaaccgaaat cctgcagtgg atctatcacg
     1141 gcaagagcaa catcgagatc ggtatgatcc tcaacatcag tgcgttgacc gtgaagaatc
     1201 acgtccagaa aatactgcgc aaattgaatg ttctgaatcg cacgcaggcg atcgggaagg
     1261 cgctcgcgtt gcgtatcctc aatccctgag gagcgcctgc tactcgacgg tcaccgactt
     1321 cgcgaggtta cgcggcttgt cgatgtcggt gcccttctcg agtgccgcgt agtacgcgag
     1381 aagctgcagc gggatcgtat gcagtatggg ggagagcagg cccgcatggt ccggcatgcg
     1441 aatcacatgc ataccttcgc cttcgtgaat gcgggtgtct gggtcggaga tcacgtggag
     1501 ttcccctccg cgcgcacgca cttcctgcag gttggactga agtttctcca gtaacgcatc
     1561 tttcggcgcg atcgctatca ccggcatttc gcgatccacg agtgcaagcg gcccgtgctt
     1621 gagttcgccg gccgcatagg cttcggcgtg tatgtaggag atttccttta gcttgagcgc
     1681 gccttccatg gcgatcggat agtggatgcc gcgtccgagg aagagcgcgt gctggcgtct
     1741 cgcgaacttt ctgccccagg cttcaatggc cgcttcgacg ccgaggacgt tgctgagcgc
     1801 agacggcaga tggcgcagcg aagcgaggag cgcagcctcc tgatcgcccg aaaggcggcc
     1861 gcgcagcttc gcgagcgtca acgtgagcag gacgaggacg gcgagctgcg tggtgaatgc
     1921 tttcgtcgac gcaaccccga tctcggggcc tgcgcgcgtg agaaagcgca gatccgccgc
     1981 gcgcacgagc gcggactcgg gaacgttgca gatcgctaga caatgccggt atccgagttt
     2041 gcgggcgtgg tgcaccgcgg ccagcgtgtc ggccgtctca ccggactggg agatcgcaac
     2101 caccaggccg cgcggattcg gtacggactc gcggtagcgg tactcgctcg cgatctcgac
     2161 gctgcaaggg atgcccgcca cgctctcgag ccagtagcgg gcaaccatgc cggcgtgata
     2221 gctcgtgccg catgccagga taagcagggc gtctgcctcg cgcaggatgc cttccgcgct
     2281 tgcgccaaag acgcctggag aaatcgaccg cgcgttggtg accatctcca gtgtattcgc
     2341 gaccgccatc ggctgctcga agatctcctt ctgcatgtga tgacgatagg gcgcgagctc
     2401 gatcgattcc gaagtgagct cgcttacgcg cacggggcgc tggcatgccg cgcctttgcc
     2461 gtccacgatg cgcacgccct tgagggtgat ttcggcgcaa tcgccttctt cgagatatac
     2521 gacgttgcgc gtcacctgaa tgagcgccgc ggcgtccgag gctgcgaaga attcactgtc
     2581 accgacgccc agaatcagcg gtgcacccat gcgggcaacc acgagacggg tgggatctgc
     2641 ttcgaacgct accgcgatgg cgtaggctcc aacgaggtct gcggtgctcg cacgcaccgc
     2701 ctcaaaaagg cttgcgcctt tgcaaacgtg cgagtgcacg aggtgtgcga tgacttctgt
     2761 atcggtctcg gacacgaact cgtatccctg cgctgcgagc gcatgacgca tggcctcgtg
     2821 gttttcgatg atgccgttgt ggaccaccga gatcccgccc gagacgtgag ggtgggcgtt
     2881 gcgttcggac ggcatgccgt gagtggccca gcgggtatga gcaatcccga tggtgccgcg
     2941 cagcccgtgc gcctgggcca gcgtcgccag cgcggcgacc cgccccgtgc tgcggagccg
     3001 ctggaggctc ccgttgatga ccgcgacgcc ggcggaatca tagccgcggt attcgaggca
     3061 ccttagcccc tcgagcagga tcggtacgac gttacgcttc gaaatggcgc cgacaattcc
     3121 acacatatcg cttgctcagc ttacgagatt ggcgcgcaat gatgcgccct gggacgattc
     3181 atgacagtgc gcgcccggac gattgagggc ggcgcgcttc agtcgtactc cttgccgcgc
     3241 acgacataag gcgcaaagct ttctgaagta tcgtcgatgt cgacgtcgtc gaacggtgca
     3301 tcatcttcca cgtccggttc ttcggtctcg tcgaggcccc gcaattccag ctcgcgcctg
     3361 agccggctgt ccgagaatct gcggtagccg gcaaaacctt tttcaaaaag ctcctgcagc
     3421 cagtaggact gcggctcgcg cagcgcagcc tccaccgagt acgacacgag cttgcggatc
     3481 atcgcttccc tgttcatcac tgtacgtccc ctttgtggta atcgggcagg agagaggata
     3541 gggcggcgga tgtttagcac aatgcgcgat gcgggccgcg ccgctagctc gattggacta
     3601 acgcgccgca tttcatacgc atgtcacaga actcggccat agtcacgctc gagaggacga
     3661 gattcgcttg tcgcaccgct gttgtttgcc catgtcgagg ggcgccgtcg tacgggctgc
     3721 ggatttgagg cccgtcgcgt cggcagcccg tgcggtccag tccgggctct tgcggcattc
     3781 acgtcaatcg tgggggccgg aatgacagcc gagccggtgc tgaccgaacg cgaaaaggaa
     3841 attctgcatc tgatctatca ggggaagagc aacatggaaa tcgcggcggc ccttagcatt
     3901 aacacgctca ccgtcaagga tcatgtgcgc aagattctgc gcaagctcaa ggtgcacaac
     3961 cgcacccagg cggtggggcg agccgtcgcc ctgcgtatcg tgacgctgcc cgaggctcct
     4021 cagccaccta gttcaaagcg cccatccacc ctgcaccatt gatacgcacg actgtcacgc
     4081 gcctcggcca cactggagcc ggtcgctcgc ggaacccgca cggcaggtcg ctttgagggg
     4141 tgggcgggac gtctacatcg ctggatggtg catgcgtaac c
//
LOCUS       DASURG010000379         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_576404, whole genome shotgun sequence.
ACCESSION   DASURG010000379 DASURG010000000
VERSION     DASURG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576404"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2592)
                     /gene="fdhF"
                     /locus_tag="VFY80_06660"
     CDS             complement(<1..>2592)
                     /gene="fdhF"
                     /locus_tag="VFY80_06660"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX6007625.1"
                     /translation="LCYKDGMRPDGNCRACMVEIKGERVLAPSCCRQPTPGMEVISDN
                     ARARASQKMVVELLLSDMPEERHTLHSELDHWAAKLEVVKPRFARRREPPADLSHYAM
                     AVNLDSCIQCTRCVRACREEQVNDVIGYAYRSDHSKIVFDFDDPMGESTCVACGECVQ
                     ACPTGALMPARGVGMVKPDKQVHSVCPYCGVGCQLTYNIKDNRILHVEGRDGPSNRGR
                     LCVKGRYGFDYVHHRQRLTQPLIRKPGVPKSAEFTMDPANPLEYFREATWEEALDLAA
                     DRLREIRDRDGKQALAGFGSAKGTNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAA
                     LMEGLNSGAVSNQVNDVLRAEVIFLIGANPIVNHPVAATWIKNAVQRGAKLIYADPRR
                     SELSRHATYALQFKADTDVALLNAMMHTIVHEGLVNEDFIRTRTVGYDALKKNVEGYS
                     PEAMAPICGIPAETIKEVARVYATSKGSMILWGMGISQHVHGTDNARCLIALCLMTGQ
                     VGRPGTGLHPLRGQNNVQGASDSGLIPMVFPDYQRVDNAEARARFEKLWGATLDPKPG
                     LTVVEIMNAVHEGKLRGMYIMGENPAMSDPDVHHAREALAKLDWLVVQEIFLTETCYH
                     ADVILPATAWPEKVGTVTNTDRMVQLGRQALTPPGEARPDLWIIQELARRLGLDWHYD
                     GPRDVFNEMRQAMPSIAGITWKRLETESSVTYPCTKEGDPGQPVVFVDDFPTATGRAK
                     FVPADIIPAAERPDEAYPFVLITGRQLEHWHTGAITRRAAVLDAIEPDPVASIHPLDL
                     DAIGAREGDVITVESRRGIISLYARSDDGTPRGAVFIPFCYYEAAANLLTNPALDPYG
                     KIPEFKY"
BASE COUNT          447 a          842 c          812 g          491 t
ORIGIN      
        1 agtacttgaa ttccggaatc ttgccgtagg ggtcgagtgc cggattggtc agaaggttgg
       61 cggccgcttc gtaatagcag aacggaatga aaacggcacc tcgcggcgtg ccgtcgtcgg
      121 agcgtgcata gagcgaaatg atgccgcggc gcgattccac cgtaattaca tcaccctccc
      181 ttgcaccgat tgcatcgaga tcgagcgggt ggatcgaagc gaccggatcc ggttcgatcg
      241 catcgagcac tgcggcgcgt cgcgtgatgg cgccggtgtg ccagtgctcg agttgccgcc
      301 cggtgatgag cacgaaggga tacgcctcat cgggccgctc ggccgcggga atgatgtccg
      361 ccggcacgaa cttagcgcgc cccgttgccg tcgggaagtc gtccacgaag acgaccggct
      421 gcccgggatc gccttccttc gtgcagggat acgtgacgct gctctccgtt tcgagacgct
      481 tccacgtgat gccggcgatc gagggcatcg cctggcgcat ttcgttgaac acgtcgcgcg
      541 ggccgtcgta gtgccagtcg agtcccaggc gccgcgcgag ctcctggatg atccacaaat
      601 cgggcctcgc ttctcccggc ggcgtcaatg cctggcggcc gagctgaacc atgcgatcgg
      661 tattggtcac cgtgcccact ttttccggcc acgccgtggc cggcagaatg acatcggcgt
      721 gatagcacgt ttcggtaagg aaaatctcct gcaccacgag ccagtcgagc ttcgcgagcg
      781 cttcgcgcgc gtgatgcacg tcgggatcgg acatcgcggg attctcgccc atgatgtaca
      841 tgccgcgcag cttcccttcg tgcaccgcgt tcatgatttc caccaccgtc agccccggtt
      901 tcggatcgag cgtcgcgccc cacagcttct cgaatcgcgc gcgcgcttcg gcattgtcca
      961 cacgctggta atcggggaac accatgggaa tcaacccgga gtcggaggcg ccctgcacgt
     1021 tgttttgccc acgcaaggga tggagcccag tgcccggccg tccgacctgc cccgtcatga
     1081 ggcacagcgc gatcaggcag cgcgcgttgt cggtgccatg cacgtgttgc gagatgccca
     1141 tgccccagag gatcatcgag cccttcgagg ttgcatagac gcgcgcgact tccttgatcg
     1201 tctccgcggg aatgccgcag atcggcgcca tcgcttcggg gctgtagcct tccacgttct
     1261 tcttcaacgc atcgtagccg acggtgcgcg tcctgatgaa gtcttcgttc accagcccct
     1321 cgtgcacgat cgtgtgcatc atcgcgttca ggagcgcgac gtcggtatcg gccttgaact
     1381 gcagcgcgta ggtcgcgtgc cgcgataact ccgagcgccg cggatcggcg tagatcaact
     1441 tcgcgccgcg ctgcacggcg ttcttgatcc aggtcgccgc gaccgggtgg ttgacgatcg
     1501 gattcgcgcc gatgaggaag atcacttccg cacgtaacac gtcgttcacc tgattcgaga
     1561 ccgcaccgga attcaggccc tccatcaacg ctgcaaccga cgaggcgtgg cagagccgcg
     1621 tgcagtgatc cacgttgttg ctgccgaaac cggtgcgcac caatttctgg aacaggtatg
     1681 cctcctcgtt ggtgcccttt gccgagccga agcccgcgag cgcctgcttg ccgtcccgat
     1741 ctctgatctc acgcaggcgg tccgcggcaa gatcgagcgc ttcctcccag gtcgcctcgc
     1801 ggaaatactc gagcggattg gcgggatcca tcgtgaactc ggcgctcttc ggcacgcccg
     1861 gcttgcggat gagcggctgg gtgagccgct gccggtgatg cacatagtcg aatccatagc
     1921 ggcccttgac gcacagccgg ccgcggttcg aaggcccatc gcgcccttcg acgtgaagga
     1981 tgcggttgtc cttgatgtta taggtcaact ggcagccgac accgcagtac gggcacacgg
     2041 aatgcacctg tttgtcgggt ttcaccatgc cgacgccgcg cgcgggcatg agggccccgg
     2101 tgggacacgc ctgcacgcat tcgccgcacg caacacaggt cgattcgccc atcgggtcgt
     2161 cgaagtcgaa cacgatcttc gagtgatcgc tgcgataggc gtaaccgatg acatcgttca
     2221 cctgctcctc gcgacacgcg cgcacgcacc gcgtgcactg aatgcagctg tcgagattga
     2281 ccgccatcgc gtaatgcgag agatcggcgg gcggctcgcg gcggcgcgcg aagcgaggtt
     2341 tgaccacctc gagcttggcc gcccagtgat cgagctccga gtgcagcgta tggcgctcct
     2401 ccggcatatc cgaaaggagc agctccacca ccatcttctg cgaagcgcgc gcacgcgcgt
     2461 tgtcggagat cacttccatg cccggcgtgg gctggcggca gcacgatggc gcgagcacgc
     2521 gctcaccctt gatctccacc atgcaggcgc ggcagttgcc atccggccgc atgccgtcct
     2581 tgtagcagag at
//
LOCUS       DASURG010000380         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_780279, whole genome shotgun sequence.
ACCESSION   DASURG010000380 DASURG010000000
VERSION     DASURG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780279"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFY80_06665"
     CDS             complement(<1..736)
                     /locus_tag="VFY80_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007626.1"
                     /translation="MSAETQDEFEFFLRKPWSDGFPVVTPTEERMRWILEGTRRDPEE
                     LVGRIPPAMTPATVRTVAVHALMAGCLPEYLPIVLGGLALMLREEFNLNGVQGTMHGV
                     APLMIVNGPYAATIGLHGGNGCFGPGFRANASIGRAIRLMLTNLGGGIPGVGSATVFG
                     TPLRYTACVTENSEKSPWETLGVSRGYPADENVITCAMVESPRLCYDDASQEPEPLLT
                     GIADSMTAMGSWNMHVRSDMVVAMSPE"
     gene            complement(932..1900)
                     /locus_tag="VFY80_06670"
     CDS             complement(932..1900)
                     /locus_tag="VFY80_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007627.1"
                     /translation="MSWAKFAALLLSALATSAVSTQGLAQAYPSKAVRLISPYPPGGG
                     TDATARIIAQALGEQLGQQVIVDTRPGASGRIGVEIASKAPPDGYTLVLGNVAPLAIL
                     PAAVAKLPYDPIKGFQPISLIATSDYVLTVHPSLPTRSVKELLALARAKPGQLTFASS
                     GNLGAPHLAGELINQLAKVKLLHVPYKGNGPAAIAVMTGESTMLFGSGPSVVPHMKTG
                     KLRALATTGQKRSMPDLPALNELLPGYDVTQWYGILVPAGVPRDIVERLHREIARAIG
                     NAKVAQLFVNLGTQPVSNTPEEFAAFIKAETDKWGKVIRSANITAD"
     gene            complement(1921..>2645)
                     /locus_tag="VFY80_06675"
     CDS             complement(1921..>2645)
                     /locus_tag="VFY80_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6007628.1"
                     /translation="STIRPSSYCGIVGYKPSFGTINRAGLKPLAESLDTIGVMARTVE
                     DCALLVHAVSDRPLPDFTQKLAQPPRIGLHRTSRWNEASAPAQAALERAASALAKLGA
                     RVREVALPGGFDRLYDDQMLIMNFEAARALSPEYFGHRELLSPHMQKMIPEYLAKPRD
                     AYAAAMRHARECRAVLESSISDVDMLLTPGAPGEAPEGISETGSSLFNRNWTLLGVQL
                     VGGYDEDARVLRCAHWAAQVLS"
BASE COUNT          464 a          834 c          862 g          485 t
ORIGIN      
        1 gttccgggct catcgccacg accatgtcgg agcgcacgtg catgttccat gatcccatgg
       61 cagtcatcga gtccgcgatg ccggtgagca gcggctccgg ttcctggctg gcgtcgtcgt
      121 agcagaggcg cgggctctcc accatggcgc aggtgatcac attttcgtcg gctgggtagc
      181 cgcgcgatac gccgagcgtc tcccacggac tcttctcgct gttctcggtg acacacgcgg
      241 tgtagcgcag cggcgtcccg aatacggtgg cggagccgac tccgggaatg ccgccgccga
      301 ggttcgtcaa catcaggcgg atcgcgcggc cgatcgaagc gttggcacgg aagccgggcc
      361 caaaacatcc attaccgccg tgcaagccga tcgtcgccgc atagggcccg ttgacgatca
      421 tgagcggtgc gacgccgtgc atcgtgccct gtacgccgtt cagattgaat tcttcgcgca
      481 gcatgagcgc cagaccaccg agcacgatgg gaagatactc cggcaggcaa ccggccatca
      541 gcgcgtgtac ggcaaccgtg cgaacggtcg cgggcgtcat tgcgggcggg attcgcccga
      601 ccagttcctc cggatcgcgg cgtgtacctt cgagtatcca tcgcatgcgt tcttcggtgg
      661 gtgtgacgac cgggaagccg tcggaccacg gctttctcag gaagaactca aactcgtcct
      721 gtgtttcagc ggacacgttt ctccgtctcg tcgtcccagc gctatttcgc catcccggcg
      781 ccattttcgt cgttccggcg aaagccggaa cccagcaagg tacgaaaagc gactgggatt
      841 cggccgttgt ccggaatgac gaacgacgtc gttgtccgga atgacgaacg atgtccgcgt
      901 cctgagtcat gtcccattcg gccggcacct ctcagtcggc ggtgatgttg gccgagcgga
      961 tgaccttccc ccacttgtcc gtttcggcct tgatgaacgc ggcaaattct tccggggtat
     1021 tcgaaacggg ctgcgtcccg agattcacga aaagctgcgc caccttcgca ttcccgatcg
     1081 cacgtgcaat ttcacgatgc agacgctcca cgatatcgcg cggcacgccg gccggcacca
     1141 gaatgccgta ccactgcgtt acgtcgtagc cgggaaggag ttcgttcagc gcgggcagat
     1201 ccggcatcga gcgcttctgc cccgtggtcg cgagcgcgcg cagcttgccg gtcttcatat
     1261 gagggacgac ggacggcccg ctgccgaaca gcatcgtgct ctccccggtc atcaccgcaa
     1321 tcgcagccgg gccgttgcct ttgtagggaa cgtgcagcag cttcaccttc gcgagctgat
     1381 tgatgagttc tcccgcgaga tgcggcgcgc ccaggttgcc gctcgatgcg aaagtgagtt
     1441 gaccgggctt cgcgcgggcg agcgcgagca attccttgac cgagcgcgtg ggaagtgacg
     1501 ggtgcaccgt cagcacgtaa tcggaagtgg cgatgaggct gatcggctga aagcccttga
     1561 tcgggtcgta gggcagcttg gcgacagctg cgggcagaat cgcgagtggc gcgacgttgc
     1621 cgagcacgag cgtgtagccg tcgggcggcg ccttggaggc gatctccaca ccgatgcgtc
     1681 cgctcgctcc cggacgcgtg tccacaatga cctgctggcc gagctgctcg cccaacgctt
     1741 gcgcgatgat gcgcgcggtc gcatcggttc cgccaccggg cggatacggt gagatcaggc
     1801 gtaccgcctt ggaagggtag gcctgagcaa ggccttgcgt ggagacagcg ctcgttgcaa
     1861 gagcggacag caaaagtgcg gcaaacttcg cccagctcat catgatcctc ctcgctttat
     1921 ttaagagagt acctgcgcgg cccagtgagc gcagcgcagg acgcgcgcat cctcatcgta
     1981 gccgcccacg agctgcacgc cgagcagcgt ccagttgcga ttgaatagcg agcttccagt
     2041 ttcactgatg ccttccgggg cctcgcccgg cgcgccgggg gtgagcagca tatcgacgtc
     2101 cgagatcgag ctttcgagaa ccgctcggca ttcgcgcgca tgacgcatgg cggcggcata
     2161 ggcatcccgc ggtttcgcca ggtactccgg aatcatcttc tgcatgtgcg ggctgagcaa
     2221 ctcgcgatga ccgaagtatt ccggcgagag ggcgcgtgcg gcctcgaagt tcatgatgag
     2281 catctgatcg tcgtagaggc gatcgaaacc cccgggcaga gcgacctcgc gcacgcgtgc
     2341 gccgagcttc gccagtgccg atgcggcacg ctccagcgcc gcctgcgcgg gcgcactcgc
     2401 ttcgttccag cgcgaagtgc gatgcagccc gatgcgcggc ggctgcgcga gcttctgtgt
     2461 gaaatcgggg agcggtcggt ccgagaccgc gtgcacaagc agcgcacagt cctccaccgt
     2521 gcgggccatg acgccgatcg tgtcgagcga ttcggcgagc ggcttcagtc cggcgcggtt
     2581 gatagtgccg aaactcggct tgtaaccgac gatgccgcag taggaagacg gccggatcgt
     2641 ggatc
//
LOCUS       DASURG010000381         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_985052, whole genome shotgun sequence.
ACCESSION   DASURG010000381 DASURG010000000
VERSION     DASURG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985052"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFY80_06680"
     CDS             <1..651
                     /locus_tag="VFY80_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6007629.1"
                     /translation="KTMLMTYRTRSMLGFVLIAAQAFLYNAIFFTYALVLTRFYRIPA
                     EDTGIYLLPFALGNFLGPLVLGHLFDSIGRRTMIAATYALSGALLLFTGMLFVQETLS
                     ALMQTLMWSAIFFFASAAASSAYLTVSEVFPLELRALAIAIFFSVGTAAGGILAPWFF
                     GLLIGTGSRTAVFFGYALGSSLMLLAALTAARYAVTAERTPLERVARPLSADGGRD"
     gene            complement(664..1176)
                     /locus_tag="VFY80_06685"
     CDS             complement(664..1176)
                     /locus_tag="VFY80_06685"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX6007630.1"
                     /translation="MNKVVVSTLLSAALVAAAPLALSQSAGNAPHARHAAAGSPHGER
                     AFRMPSERVEARLAYLRTALKITDAQQAQWNAFADTLRKQAKDMDASVQARRAQRAEG
                     KRDARPTAIERLAHRQQRLALASARLDELLAAAKPLYAALTPEQQKVADELLGQRHRG
                     MRHGHRRGHA"
     gene            1398..2273
                     /locus_tag="VFY80_06690"
     CDS             1398..2273
                     /locus_tag="VFY80_06690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6007631.1"
                     /translation="MLIVDAQVHIWGANTPERPWPARLEAHRDVPIDQDELRREMDAA
                     GVDRVVIVPPSWEGDRNDLALAAAAAHPDRFAVMGRLDSEAAEARDRLRTWRAQPGML
                     GLRFTFHAPMLLPPLTEGRMDWVWAEAERLDIPIMVLVSHAHAHLIDRVAERHPGLRL
                     VMDHLALTRLTYDEEAFRDLDRLLALARRPNVAVKATALPCYTRDTYPYRKLHPYIRR
                     VYDAFGPQRMFWGTDLSRLPCTYRQGVTMFTEEIPWLSAEDKSWIMGRGVCEWLGWTL
                     PARVAKARVNQGRAR"
     gene            2270..3241
                     /locus_tag="VFY80_06695"
     CDS             2270..3241
                     /locus_tag="VFY80_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6007632.1"
                     /translation="MKSCWIKTDHDKAVLEFRDVPVPQPAPGQLVVRVYASALNRGEL
                     IVGGVVHGGPEKIGGNEVSGLVHAVAEGVTGIAVGARVFGRARGGFAEYALMEAHQAM
                     PIPARLSWEQAAAIPVSFLTAYEMLYPYGGLRRGDWLLATAASSGAGVASIQIARMLG
                     AHTIGTSGSAEKLARLSAIGLEHGIATRGGDFASRVKELSGGKGVDLVVNCVGGSVFG
                     ECMRALAYKGRIATVGYVDGVLKSEIDLGALHANRYVVFGVSNARLSAEGRAGAVSDF
                     ARDVLPAIADGRITPLIDKVYAFDALPEAKARMESNAQVGKIVVRIA"
BASE COUNT          514 a         1056 c         1112 g          628 t
ORIGIN      
        1 aagacgatgc ttatgactta ccgcacgcgt tccatgctcg gcttcgtcct catcgccgca
       61 caggccttcc tctacaacgc catcttcttc acctatgcac tcgtgctgac gcgtttctat
      121 cgcattcccg ccgaggacac cggcatctac cttctcccgt ttgcgctcgg caacttcctc
      181 gggccgcttg tgctgggtca cctgttcgac agcataggcc ggcgcacgat gatcgcagcg
      241 acctacgcgc tgtccggggc gctgctcctc ttcaccggaa tgcttttcgt gcaggaaacc
      301 ctctcggcgc tcatgcagac gctgatgtgg agcgcgatct tcttttttgc ttccgctgcg
      361 gcgagctccg catacctgac ggtcagtgaa gtcttccccc tcgaattgcg tgcgctcgcg
      421 atcgccattt tcttttcggt cggcacggcg gcgggtggca tattggcacc atggttcttc
      481 gggctcttga ttggtaccgg ctcgcgcacc gcggtctttt tcggctatgc cctcggctcg
      541 agcctgatgc tccttgccgc gctgaccgcc gcccgctatg ccgttacagc ggaacgcaca
      601 ccgctcgaac gcgtggcgcg cccgctgtcg gcagacggcg ggcgcgactg accacgaaga
      661 cgcttatgcg tggccgcggc gatgtccgtg acgcatgccg cggtggcgct gccccagcag
      721 ttcatcggca accttctgct gttcgggcgt gagcgctgcg tagagcggct tcgccgccgc
      781 aagcagctcg tccaagcgcg cgctggcaag cgccagccgt tgctggcggt gtgcgagccg
      841 ctcgatcgcg gtgggccgtg catcgcgctt gccttccgcg cgttgcgccc gacgtgcctg
      901 aacgctcgca tccatatcct tcgcctgctt gcgtagcgtg tcggcaaacg cgttccactg
      961 cgcttgctgc gcgtccgtga tcttgagtgc ggtcctgagg taagcgaggc gtgcctccac
     1021 gcgttcgctc ggcatgcgga acgcccgctc accgtgcggg gatcctgcgg ccgcgtggcg
     1081 ggcatgcggc gcgttgccgg cagattgcga gagcgcgagc ggcgcggctg cgacgagcgc
     1141 ggcggaaagc agggtgctga ctacaacctt attcatgatg ggcctccaat gttgttgacg
     1201 gtccgagccg cgtttggcca aattccctcc ggtgaactgc cggctcgcaa tgctccgacc
     1261 cggttgcggc agacgcggtt cctgctttgc gctgtaacag tttgtgtctt tgtaacgcag
     1321 atgacacgtc acgccgcacg cggcgttccg ccgtattgtg cgagaatcgc gagcggccgc
     1381 ataaaaagga acaacgcatg ctgattgtcg atgcgcaggt gcacatctgg ggcgccaata
     1441 cgccggagcg cccctggcct gcgcggctcg aggcacaccg cgacgtcccg attgaccagg
     1501 acgagctccg gcgggagatg gatgccgccg gcgtcgatcg ggtggtcatc gtgccgccgt
     1561 cgtgggaggg cgatcgcaac gatctcgcac tcgccgcggc agcggcgcat cccgaccggt
     1621 tcgccgtcat gggcaggctc gacagcgagg ccgcggaagc gcgcgaccgc ctgcgcacct
     1681 ggcgagccca acccgggatg ctgggcttga ggttcacgtt tcatgcgccg atgctgctcc
     1741 ctccgctgac cgaaggacgc atggactggg tatgggcgga agccgaacgg ctcgacatcc
     1801 cgatcatggt gctcgtgagc catgcgcacg cgcacctcat cgaccgcgtg gcggagcgtc
     1861 atcccggctt gaggctcgtc atggatcacc tcgcgctcac gcgccttacc tatgacgagg
     1921 aggcgttccg cgacctggac aggctgcttg cgctcgcgcg acggccgaac gtcgcggtga
     1981 aggcgactgc gcttccgtgc tacacgcgcg atacctaccc ctatcgcaag ctgcatccct
     2041 atatccggcg cgtgtacgac gcgttcggcc cgcagcgcat gttctggggt acggacctct
     2101 cgcggctgcc ctgcacgtat cgccagggtg tgacgatgtt caccgaagag attccctggt
     2161 tgagcgccga ggataagagc tggatcatgg ggcgtggcgt atgcgaatgg ctgggctgga
     2221 ccctgccagc gcgtgttgcc aaagcgcggg taaatcaagg gagagcgaga tgaagtcatg
     2281 ctggatcaag acggatcatg acaaggccgt gctggaattc cgcgatgtgc cggtgccgca
     2341 gcccgcgccc ggccagctcg tagtgcgcgt gtacgcgagc gcgctcaacc gcggcgagct
     2401 tatcgttggt ggcgtggtgc acggcggtcc ggagaagatc ggcggcaacg aggtgtccgg
     2461 gctcgtgcac gcagtggcag agggcgtgac cggtatcgca gtcggcgccc gcgtgttcgg
     2521 ccgcgcgcgc ggtggcttcg ccgagtacgc gctcatggaa gcccaccagg caatgccgat
     2581 tccggcgcgc ttgagctggg aacaggccgc cgcgatcccg gtgagctttc tcaccgcgta
     2641 tgagatgctc tacccctatg gcgggctgcg gcgcggcgac tggctcctcg ccacggccgc
     2701 atcttcgggc gccggagtcg cgagcatcca gatcgcccgc atgctcggcg cacacacgat
     2761 cggcacgtcg ggttcggcgg agaagctcgc gcgcttgagc gcaattggtc tcgagcacgg
     2821 gatcgcaacg cgtggcggtg atttcgcttc gcgggtgaag gagctctcag gcggaaaggg
     2881 cgtcgacctt gtcgtgaact gcgtcggcgg ctcagtgttt ggcgaatgca tgcgtgcgct
     2941 cgcctacaag ggacgcatcg cgaccgtcgg ttacgtggac ggagtgctga agagcgaaat
     3001 cgacctcggc gcgctccatg caaaccgtta tgtcgtcttc ggcgtctcga atgcgcggct
     3061 ttctgcggaa ggacgggcgg gtgccgtcag tgacttcgcg cgcgatgtgc tgcctgcgat
     3121 tgccgacggg cgcatcacgc cgctgatcga caaggtgtac gcgttcgatg cgctgccgga
     3181 ggcgaaggcg cgcatggaat ctaacgctca ggtcgggaag atcgtggtgc gcatcgcatg
     3241 aagcagcgct tcgaccgcgg agcgcagttg aagaacgggg cgggaagatc agagcaagtc
     3301 cggccggtac
//
LOCUS       DASURG010000382         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_65886, whole genome shotgun sequence.
ACCESSION   DASURG010000382 DASURG010000000
VERSION     DASURG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65886"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..617
                     /locus_tag="VFY80_06700"
     CDS             210..617
                     /locus_tag="VFY80_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6007633.1"
                     /translation="MPWSQTSPMDQKTQFIADYLREVLSITELCELYGISCKTAYKWI
                     DRYLRQGPAGLEDRSRVPKSSPNHTPAAIEQAILQARQRHPSWGAKKLLTLVHKAHPR
                     WELPHRSTVCEILSRNGMVCSATIWMRGARQSR"
     gene            complement(675..986)
                     /locus_tag="VFY80_06705"
     CDS             complement(675..986)
                     /locus_tag="VFY80_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007634.1"
                     /translation="MSANDAQANSVTRDATAEAAVPAIKRSAAQRQRAYRLRRKRAAI
                     EAIGEESAASRVALMTLLAYELAALDARGTPANLIASRRGSVKRILNAIVTRYAIDLS
                     D"
     gene            complement(983..1813)
                     /gene="istB"
                     /locus_tag="VFY80_06710"
     CDS             complement(983..1813)
                     /gene="istB"
                     /locus_tag="VFY80_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX6007635.1"
                     /translation="MSNAIDTSRLSLLLNELRLPALKHIWPSFAERSDKEGWPAARLL
                     MALAEHEIAERDRRRIERHLAEAKLLPGKTLENFDFEAVPMLSKAQVSALCAGDGWIR
                     NGANLLLVGPPGGGKSHLSSAIGLSLLEKGWKVLFTRTSDLVQRLQVARRELALEAAI
                     NRLDRFDLVILDDLAYVTKDQAETSVLFELISARYERRSLLITANQPFGEWNKVFPDP
                     AMTLAAVDRLVHHATIFELNVESYRRRSALLSKQKGPGRPAHFATPKNVGVPRKEKSQ
                     "
     gene            complement(1810..2003)
                     /locus_tag="VFY80_06715"
                     /pseudo
     CDS             complement(1810..2003)
                     /locus_tag="VFY80_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253211.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          353 a          598 c          693 g          359 t
ORIGIN      
        1 ttccatgcgg acgagcgtcg tggctgagtg accgccggag cgcgaagcgc agaggggacc
       61 caacagcaag cgaagcgcct ttgtggccgg tccactccag ccaccggtta gccgttccca
      121 tcggtccggt ctggggacat gggtaacact atagtcgggt gacataggta acagtttagc
      181 cacactgagg ggcaccgagg aggtgcccca tgccgtggag tcaaacgtca cctatggacc
      241 agaagacaca attcatcgcc gactacttgc gcgaggttct gagcatcacg gaactgtgcg
      301 agctctacgg catatcgtgc aagaccgcgt acaaatggat tgaccgatat ctgcgccaag
      361 gcccagcagg gctggaagat cgatcgcgcg tcccaaagag ctcacccaat cacacgcccg
      421 cagcgatcga gcaggccatc ctgcaagccc gccagcgcca tccttcctgg ggtgcgaaga
      481 agctcttgac tctggtgcac aaggctcatc cgcgctggga gttgccacac cgttcgacgg
      541 tgtgtgagat cctgagccgc aacgggatgg tgtgtagcgc gacgatctgg atgagaggcg
      601 cgcgacaatc aagatgagaa tgtcgcggcg gatcgatgat gccggcgttg attgacgctg
      661 gcaagggtga tgcatcagtc gctcaggtcg atggcgtaac gcgtgacgat ggcgttcaag
      721 atgcgcttga ccgagccacg tctggacgcg atcaggttcg ccggtgtacc gcgcgcgtcc
      781 aatgcggcaa gctcgtaagc gagcagtgtc atcaacgcca cgcgtgaagc cgcggattct
      841 tctccgatgg cctcgatggc agcacgcttg cgtcgcaggc ggtaggcgcg ctgtcgctgg
      901 gcggcgctgc gtttgatggc gggcacggcg gcttcggcgg tcgcgtcacg cgttacgctg
      961 ttggcttgcg cgtcgtttgc gctcattggc tcttctcctt gcgcggtacg ccgacgttct
     1021 tcggggtggc gaagtgggcg gggcgtccgg gccccttttg cttgctgagc agggcgctgc
     1081 gtcggcgata gctctcgacg ttgagctcga agatggttgc gtggtggacc aggcggtcga
     1141 cggcggcgag cgtcatggcg ggatccggga agaccttgtt ccattcgccg aagggctggt
     1201 tggcggtaat gagcagggag cggcgctcgt agcgggcgct gatcaactcg aacaacaccg
     1261 acgtctcggc ctggtccttg gtgacgtagg ccagatcgtc gaggatgacc agatcgaagc
     1321 ggtcgagccg gttgatggcc gcttccaggg cgagctcgcg gcgggcgacc tgcaggcgct
     1381 ggaccaggtc cgaggtgcgg gtgaagagca ccttccagcc cttctcgagc aggctcagtc
     1441 cgatggccga ggacaggtgc gacttgccgc caccgggggg accgacaagg agcagattgg
     1501 cgccgttgcg aatccacccg tcgccggcgc acagcgccga gacctgcgcc ttggagagca
     1561 tcggcaccgc ctcgaagtcg aagttctcca gcgtcttgcc cggcagcagc ttcgcttctg
     1621 cgaggtggcg ttcaatgcgg cgccgatcgc gctcggcgat ctcgtgctcg gccagcgcca
     1681 tgagcaggcg cgccgccggc cagccttcct tgtcggagcg ctcggcgaag ctcggccaga
     1741 tgtgcttgag cgcaggaagc cgcagttcgt tgagcagcaa agagagccgc gaggtatcga
     1801 tagcgttgct cataccgaca cctccacgtc cgggtcgatc agggcctcgt agtcgagcag
     1861 cgaagcgagt tgcacgtgta cttgcggcag gcgcgccggg tctgggccga agcgcgcagg
     1921 gcattcagat ccggcagctg tccctgggca aggcactgct tgagcgcctc ggccacttgc
     1981 ccctcgcagg tgcgctcgtg cgc
//
LOCUS       DASURG010000383         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_679175, whole genome shotgun sequence.
ACCESSION   DASURG010000383 DASURG010000000
VERSION     DASURG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679175"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFY80_06720"
     CDS             complement(<1..764)
                     /locus_tag="VFY80_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6007636.1"
                     /translation="MYETILWEVADHVALLTLNRPERLNAVDNAMRQELEDVMRRAEA
                     DNDIWTIIVTGAGRGFCSGADLKARAEAEKRGESAAHPSLFEPRYYYAIAFSETTKPV
                     IAAINGVTRGAGCNMAWAADFRIASERANFGVNFVERGLMGETSAYYLPRLVGHAVAA
                     EICLLGEPFDAAQAKAWGLVNAVVPHEKLIDEATALARKINAKAPLAVRATKMALRRA
                     YAYDAEQFLDLQNVMNTKLRTTSDTKEAMRAFLEKRA"
     gene            complement(781..1230)
                     /locus_tag="VFY80_06725"
     CDS             complement(781..1230)
                     /locus_tag="VFY80_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase"
                     /protein_id="HEX6007637.1"
                     /translation="MNPSFAQRIASMNAMYRLPAHERPTLPDDPADRLTKFKATLLEE
                     VHEIDEIVALAQRGAAPIDIAVAIADLLGDIVVYCRSEALKYGIPLEEVLDIIMDSNQ
                     SKLGADGNPIYDANGKFLKGPNYWRPESRIKALLDARGMNRAGERAA"
     gene            complement(1310..>2264)
                     /locus_tag="VFY80_06730"
     CDS             complement(1310..>2264)
                     /locus_tag="VFY80_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX6007638.1"
                     /translation="MPNTIVTSYNRNFPARNDGAPTTMNFIASPEIVTALAIAGRLSF
                     NPLTDTLTGADGTPFRFDPPRAAPEVPPRNFDRGQANYIAPPEDGSEVKLAVNPRSER
                     IELMQPWPAWDGKDFLDMPVLIKTKGKTTTDHISPAGPWLKFRGHLDKFSDNMFMGAI
                     NAYSGTAGRGRNAYTGETRSFAENARDYRARGAKWVVVGDANYGEGSSREHAALSPRL
                     LGGVAVIVRSFARIHESNLKKQGLLALTFQDAAHYERIREDDRVSLVGLASLAPGKPV
                     ECRVTHANGEVETLWLNHTFSTSQLGWFRAGSALNVVHKAA"
BASE COUNT          366 a          738 c          715 g          445 t
ORIGIN      
        1 gcgcgtttct ccaggaacgc gcgcatcgcc tccttcgtgt ccgaagtcgt gcgcagcttc
       61 gtattcatca cgttctgcag atcgaggaac tgctcggcat cgtaggcgta ggcccgcctg
      121 agcgccatct tggtcgcgcg caccgcgagc ggcgccttgg cgttgatctt gcgggccaag
      181 gccgtagctt cgtcgatcaa tttctcgtga ggcacaacgg cgttgacgag cccccacgct
      241 ttcgcctgcg ccgcatcgaa aggctcgccg agaagacaga tctcggcggc aaccgcatgc
      301 cccaccagtc gcggcagata gtaggccgaa gtctccccca tcagtccgcg ctcgacaaag
      361 ttgacgccga aattggcacg ctcgctcgcg atgcggaaat cggccgccca ggccatgttg
      421 caacccgcac cgcgcgtgac gccgttgatc gcggcgataa cgggcttggt cgtctcgctg
      481 aacgcaatcg cgtagtaata gcgcggctcg aaaagcgacg gatgcgctgc ggattctccg
      541 cgcttttccg cttccgcacg cgccttgaga tccgcaccgg aacaaaaacc gcggccggcg
      601 cctgtcacga tgatcgtcca gatgtcgttg tcggcttctg cgcgccgcat cacgtcctca
      661 agctcctgcc gcatcgcgtt gtcaaccgca ttgaggcgct ccggccggtt gagcgtaagg
      721 agcgcaacgt ggtccgcgac ttcccacagt atcgtctcgt acatggatcg ctccctgccc
      781 tcaggccgcg cgttcgcccg cgcggttcat cccccgcgca tcgagcagcg ccttgatcct
      841 ggactcgggc ctccagtagt tgggaccctt caggaatttt ccgttcgcgt catagatggg
      901 attgccgtcc gcgccgagct tgctctggtt gctgtccatg atgatgtcga gcacctcttc
      961 gagcggaatg ccgtacttga gcgcttcgga tcggcagtag accacaatgt cccccaggag
     1021 atcggctatg gcgacggcga tgtcgatcgg agcggcgccg cgctgcgcga gcgcgacgat
     1081 ctcgtcgatc tcgtgcactt cctcgagcag cgtcgccttg aacttcgtga gccggtcggc
     1141 gggatcgtcg ggcaatgtcg gccgctcgtg ggccgggagg cggtacatcg cgttcatcga
     1201 cgcgatgcgc tgtgcaaaag agggattcat gggatcggga cggatttatt gggtgcgcac
     1261 gcgcaaggat actacagcgc gccgggcccg gcccaatccc gggtggactc tatgccgctt
     1321 tgtgcaccac gttgagcgcg gaacccgcgc ggaaccaccc gagctgagag gtgctgaagg
     1381 tgtgattgag ccacagcgtc tcgacttcgc cgttcgcgtg cgtcacgcga cactccaccg
     1441 gcttccccgg cgcgaggctc gcaaggccga cgaggctcac cctgtcgtcc tcgcgtatgc
     1501 gctcgtaatg tgccgcgtcc tggaacgtca gtgcgagcag tccctgcttc ttgagattcg
     1561 actcatggat gcgcgcgaag ctgcgcacga tcacggccac tccaccgaga agccgaggcg
     1621 aaagcgccgc gtgctcgcgg ctgctgcctt cgccgtagtt ggcatccccc accaccaccc
     1681 atttcgcgcc acgggcgcgg tagtcccgcg cattctcggc gaagcttcgc gtctcccccg
     1741 tgtaggcgtt ccgcccccgg cccgcggtgc cgctgtaggc gttgatcgcg cccatgaaca
     1801 tgttgtcgct gaacttgtcg aggtggccgc gaaacttaag ccacggcccg gccggtgaaa
     1861 tgtggtctgt ggtcgtcttg cccttcgtct tgatgagcac gggcatgtcg agaaagtcct
     1921 ttccatccca tgcgggccac ggctgcatga gttcaatgcg ctcgctccga ggattcacgg
     1981 cgagcttcac ttcgctcccg tcctcgggcg gtgcgatgta gttggcttgc ccgcgatcga
     2041 aattgcgcgg cggcacttcg ggtgccgctc gcggcggatc aaagcggaag ggcgtgccgt
     2101 ccgcgccggt caacgtatcg gtcagcggat tgaacgagag ccggccggca atcgcgagcg
     2161 ccgtgacgat ctccggactc gcgatgaagt tcatggtggt cggcgcgcca tcgttgcgcg
     2221 ccgggaaatt gcggttgtag gacgtcacga tggtattggg catc
//
LOCUS       DASURG010000384         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1292017, whole genome shotgun sequence.
ACCESSION   DASURG010000384 DASURG010000000
VERSION     DASURG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292017"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..706)
                     /locus_tag="VFY80_06735"
     CDS             complement(152..706)
                     /locus_tag="VFY80_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HEX6007639.1"
                     /translation="MVDIDNYAHHAVDGSGAELKEVVLGLHWDPPQEDADTRPADLDA
                     VCMLFGMHGKSLEVVHPGNPRSRDGAVIHTGDSRTGASQWDDERIFVFLNALPQSVVA
                     LTFVVASPAGLPLAEVRGASCHLSDHASEEELLRVEFAGLQPATAYRIATLTRSAAGW
                     RISTDAHPLPEPLKAIRRLQSGSA"
     gene            936..1991
                     /locus_tag="VFY80_06740"
     CDS             936..1991
                     /locus_tag="VFY80_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX6007640.1"
                     /translation="MKILVLAGDGIGPEITDATLQVLERASVRFRLGLEWQREAIGMA
                     ALARSGSTLPGAVMDAARAAPGIILGPVDHLRYPPREQGGINPSGEFRVKLDLFANIR
                     PAKSRMGVGLTGKPVDLVIFRECTEGFYADRNMYQGIGEFMPTEDLALSVRRITARQS
                     RRIAEVAFEWARRRRRKVTAVHKANVLHVTEGLFLREVRKVAERFPDCALEEVIVDAM
                     AALLLRDPQRFDCVVTTNMYGDILSDEASELSGSLGLAGSVMAGDGRCCAQAQHGSAP
                     DIAGQDKANPTSLILSAAMLLDWLGSRHARPEFAQAAQTIESAVDQALQDAATRTADL
                     GGPLGTRAFGAAIAARI"
     gene            complement(2105..>2367)
                     /locus_tag="VFY80_06745"
     CDS             complement(2105..>2367)
                     /locus_tag="VFY80_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6007641.1"
                     /translation="DSELGRKGGAGPGREGRNLDHLCLRVEPFDSGGIAAHLIRHGVV
                     PGETRMRYGAEGEGPSMYLSDPEGNTVELKGPPAREARQRRR"
BASE COUNT          449 a          771 c          745 g          402 t
ORIGIN      
        1 gtgcccgtgg ccggcaagaa gatctacggc ctgcaatagt cgaaggcatg acatccgctt
       61 taccgcgtcg caggccgcga cgaaacggct atggcgggcg ccggtcgaaa ggcgcgccta
      121 gcccctgtat cgtgcagtga gtcggtaagg attatgcgga tcctgattgc aaacgacgga
      181 tcgccttcag cggttcaggc agcggatgcg catccgtgga aattctccaa cctgcagcac
      241 tgcgggtgag cgtggcaatg cggtaggcgg tggcaggctg gagacccgcg aactccactc
      301 gcagcagctc ctcctccgac gcatggtcgc taaggtgaca cgatgcacca cgtacctccg
      361 caagcggcag tccggcggga ctcgccacga caaaagtcag cgcgacgacc gactggggaa
      421 gcgcgttaag gaacacgaaa atgcgttcgt catcccattg gctggcaccg gtgcgcgagt
      481 cgccggtatg aatgaccgcg ccgtcccggc tgcgcgggtt ccctggatga accacttcaa
      541 gagatttacc gtgcatcccg aacaacatgc acacggcatc gagatcggcg ggtcgcgtgt
      601 ctgcatcctc ctgcggtgga tcccagtgca aacccaagac gacttccttg agttcagctc
      661 cggacccatc aactgcgtgg tgagcgtagt tgtcgatgtc caccatcctc ccttatcaga
      721 gcataaacgc gagcggatgt cagaccagca gccgctttgg aagttccaac agcgccactg
      781 ttccgcattg ccgacgtagc agcagcattg atctacgtcg acatgagcgc tccggccacc
      841 gtgaaaattt cgactcacga cgaacgcgga gatgggcggt tccttgccct ttcgcacatc
      901 tgtagacttt tcacgtatcc tgtcgaaaac ggcgcatgaa aatcctggta ctcgcgggcg
      961 atggcatcgg gcccgaaatt accgatgcga cactgcaggt gctcgagcgt gcgagcgtgc
     1021 gcttcaggct cgggctcgaa tggcagcgcg aagcgatcgg aatggcggcg ctcgccagat
     1081 ccggttcgac actccccggc gcggtcatgg acgccgcgcg tgcagcgccc ggcatcatcc
     1141 tcggtcccgt cgatcacctg cgctatccgc cgcgcgagca gggcggtatc aacccgtcgg
     1201 gcgaattccg tgtgaagctg gatctttttg cgaacatccg cccggcgaaa tcgcgcatgg
     1261 gcgttggcct caccggaaag ccagtcgatc tcgtgatctt ccgcgagtgc accgaaggtt
     1321 tctacgccga ccgcaatatg taccagggca tcggcgaatt catgccgacc gaggacctcg
     1381 cgctgtcggt gcgccggatc acggcccgcc agtcgcggcg catcgcggaa gtggccttcg
     1441 aatgggcacg gaggcggcgc aggaaagtga cggcggtgca caaggcgaac gtgctgcacg
     1501 tgaccgaagg gcttttcctg cgcgaagtgc gcaaggtcgc ggagcggttt cccgactgcg
     1561 cgctggagga agtgatcgtc gacgcgatgg cggcgctctt gctgcgcgac ccgcagcgct
     1621 tcgactgcgt cgtcacgacg aacatgtacg gtgacatcct gtccgacgaa gcctccgagc
     1681 tttccggcag cctcgggctt gcgggatcgg tcatggcggg agacgggcgc tgctgcgcgc
     1741 aggcgcagca cggcagcgcg cccgatatcg ccggacaaga caaggccaat cccacctcgc
     1801 tcatcctgtc ggcggccatg ctgctcgact ggctcgggag ccgccatgca cgaccggaat
     1861 ttgcacaagc cgcgcagacg atcgaaagcg cagtcgatca agcgctgcag gacgccgcca
     1921 cgcgcacggc cgacctcggc ggcccattgg gaacgcgcgc attcggcgcc gcaatcgccg
     1981 cgcgaatata agcagtgagc gggtgaagcg tgaagcgcgc aattgcgagg cacgtcgtgc
     2041 cggtgaaaat cggcatccat atctatgccg ttccggattc cgccggaatg acacggggtg
     2101 ccagtcagcg ccgccgctga cgcgcttccc gcgcaggcgg gcccttgagc tccaccgtgt
     2161 tcccttcggg gtcgctcagg tacatcgacg gcccctcgcc ttccgcgcca tagcgcatcc
     2221 tcgtctcacc gggcaccacg ccatgccgga tgagatgcgc cgcgatgccg cccgaatcga
     2281 acggctccac acggagacac aggtgatcca ggttgcggcc ctcgcgcccg ggacccgcgc
     2341 cgcctttcct acccagctcg ctgtcca
//
LOCUS       DASURG010000385         4402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_474699, whole genome shotgun sequence.
ACCESSION   DASURG010000385 DASURG010000000
VERSION     DASURG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474699"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFY80_06750"
     CDS             complement(<1..623)
                     /locus_tag="VFY80_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007642.1"
                     /translation="MSYLAREFRAVGTELPIVAFARLALAPFVCGVTLMLCMLVYGEP
                     LTERYVVLTVVVMLVALRVFGEMPLTNGRSLLIPGRSVLTDWMTVAGMLLFVGFITKT
                     SDLYSRKLLLTWFVMTPFALHVGQELARQLLCRFVSVGALARTKVIVGLNEMGHELAR
                     EIAEDPCRGVLKGFFDDRGDGRIAPFETHPLLGGVEEVARYVKENAI"
     gene            1148..2440
                     /gene="epsL"
                     /locus_tag="VFY80_06755"
     CDS             1148..2440
                     /gene="epsL"
                     /locus_tag="VFY80_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated polysaccharide
                     biosynthesis outer membrane protein EpsL"
                     /protein_id="HEX6007643.1"
                     /translation="MRILRTAGIAAMLCLPAVAAGFEMTEDILWPDDGRFPAYPQEPD
                     PRTVQFFVSGGLYHDDNIFRLSDSTVVPAGVSKSDTIYRIGARLRADVPISRQRIVLD
                     GRVDNYTFDKNGLLDHVDYRGTARWLWQAGPLFTGDLGYSRRRYLGDLGEINAPLKDM
                     ITEDRIFGSAGFMVTPRWRVRGAADWGEWDHSAATRTTLNLEVTSVTVGLDYVTPLNN
                     HLGLQHKQSHGKYPNRQPITGTTTVANSYDEGETSAVLRWNITGKSVLNARAGYTKRE
                     HDEVPARDFDGFTGRVNLDWEAGAKTILHGAVWREIRSVDELATLEQAAASYVLSTGL
                     SIGPSWAPTSKLVFQASYIHEKREYEGDPGAAFGVFPGTPGVQREDTFNGLRLAAGYA
                     PRRNIRVSLAYERGDRDSNITLRDYEYNRVSANARFQF"
     gene            complement(2530..4095)
                     /locus_tag="VFY80_06760"
     CDS             complement(2530..4095)
                     /locus_tag="VFY80_06760"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX6007644.1"
                     /translation="MKIQPVLLCGGSGTRLWPLSREQYPKQLLALNGDRTLLQATALR
                     AMGLTGEVAAPIVVCNEEHSFLVAEQLREIDVRARAIMLEPVGRGTAPALTLAALNAA
                     GEDPVLLVMPSDHVIAAEPAFCAALGVGAALAAEGFVVTFGVPPVAAETGYGYIRTGA
                     KIAASAEPAAYQVHSFVEKPDTANAKRYCASGRYLWNSGIFAVRASVWLESITRYRPE
                     IAAACERAQALGTWDSDFFRVDRKCFAECASDSIDYAVMERLEPAPASAAAPRAAVVR
                     MEAGWSDVGAWSALWEIGSKDDSGNVLHGDVCAVDTRNSLLIGQSRLIACVGLDDIVV
                     IETPDAVMVARKDKAQDVKEVVTRLKEAGRKECLNHRKVYRPWGSYDGIDSGQRFQVK
                     RIIVKPGAALSLQMHHHRAEHWVVVRGTARITRGDEVFLLTENQSTYIPLGVRHRLEN
                     PGKVALEIIEVQSGAYLGEDDIVRFDDAYGRTEKSRPIGWAPPADAALRPPRPSMDGS
                     AGGEQGVIAEVRN"
     gene            complement(4212..>4402)
                     /locus_tag="VFY80_06765"
     CDS             complement(4212..>4402)
                     /locus_tag="VFY80_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007645.1"
                     /translation="RPGHRGCRPAATGQSLTKQPDNVRIDPADPLIRAYWILIEKRAV
                     WFPTPRPAFQRILRGIHY"
BASE COUNT          816 a         1454 c         1384 g          748 t
ORIGIN      
        1 tgtatggcgt tttccttcac gtaacgcgcc acttcctcca cgccgcccag cagcggatgc
       61 gtttcgaacg gcgcgatgcg cccgtcgccg cgatcgtcga agaagccctt gagcacgccg
      121 cggcacgggt cttcggcgat ctctcgtgca agctcatgcc ccatttcgtt caagccgacg
      181 atgaccttgg tgcgtgcgag cgcgccgacc gatacgaagc ggcagaggag ctgccgtgcc
      241 agttcctgcc cgacatgcaa ggcgaaaggc gtcatgacaa accacgtcag gagcagcttg
      301 cgcgagtaga gatccgaggt tttggtaatg aatccaacga agagcagcat gccggcgacg
      361 gtcatccagt cggtcagcac gctgcgcccg ggaatgagca aggatcggcc gttcgtgagc
      421 ggcatttcgc cgaagacacg caaggcgacc agcatgacga ccacggtcag cacgacatac
      481 cgttcggtga gcggctcccc gtagaccagc atgcacagca tcaaggtgac accgcaaacg
      541 aagggcgcga gggcgagcct cgcaaacgcg acgatgggca gctcggtgcc gaccgcccga
      601 aattcgcgcg caaggtagct catggggccc gccagtccaa catgatggtc cgcatatcct
      661 tccgcgggac gcgttgccgc atcccggcgc ctgccgctgt ccatgaagcc ggacgcactc
      721 cgcgccggca ccgggcacga tttccggtac aagaccccgc tcccccgtgg tcgtctacat
      781 gccaacccgt gctgccagca actgccatgc cagaagcgca agtgcgttgc gactcagatg
      841 cttccggcat gaaggggtcc cggagccggg ccgcaaaccg tggcattctc gtcaccattg
      901 ggcgacagac ctccggcgat ggggcagagg tttttgattt gcttcggaat gttctgtcgc
      961 attcgagcaa cagaacgccg cttgccgagg ctgtcgcgct ggccatttgc ctgaatgcgc
     1021 aggcataatg ctgcaggcta tccggcgcgg tcctgcatgg gtgcggcctg ccggtatcaa
     1081 aaagaacggc gcgggcccgc aggcatgacg ccttcgacct tcgcgccctg aatgaggaaa
     1141 aaaagcgatg cgcatcctga gaacagcggg tatagccgcc atgctctgtc tgcccgcggt
     1201 cgccgcgggc ttcgagatga cggaagacat actctggccg gacgacgggc gcttccccgc
     1261 ctatccgcag gagccggatc cgcgcacggt gcagttcttc gtatccggcg gcctctacca
     1321 cgacgacaac atcttcaggc tttcggacag cacggtcgtt cccgcgggcg tgagcaagtc
     1381 cgacacgatt taccgcatcg gcgcacggtt gcgcgccgac gtaccgatca gccgccagcg
     1441 catcgttctg gacggacggg tcgacaacta caccttcgac aaaaacggcc tgctcgatca
     1501 cgtcgattac cgtggaacgg cgcgctggtt gtggcaggcg ggtccgcttt tcaccggcga
     1561 tctcgggtat agccggcgcc gctatctcgg cgacctcggg gaaatcaacg caccgctcaa
     1621 ggatatgatc acggaggatc gtattttcgg cagcgcgggc ttcatggtga cgccgcgctg
     1681 gcgcgtgcgg ggtgcggcgg actggggcga atgggatcac agcgcggcga cgcgcacgac
     1741 cctcaatctc gaagtgacgt cggtcaccgt gggcctggac tacgtgacgc cgctcaacaa
     1801 ccacctcggg ttgcagcaca agcagagcca cggcaagtat ccgaaccgcc agcccatcac
     1861 ggggacgacc accgttgcga acagctatga cgagggtgag acgagcgcgg tcctgcgctg
     1921 gaatatcacg ggcaaatcgg tgctcaatgc gcgcgccggg tatacgaagc gcgagcacga
     1981 tgaagtgcct gcgcgcgact tcgacggctt caccggccgc gtgaacctcg actgggaagc
     2041 gggcgcaaaa accatattgc atggtgccgt atggcgcgag atccgctccg tggatgaact
     2101 ggcgacgctg gagcaggccg ccgccagtta tgtcttgagc acgggactca gcatcggccc
     2161 gtcgtgggcg cccacgtcca agctcgtgtt ccaggcgagt tatatccatg aaaaacggga
     2221 gtacgaaggc gatcccggcg ccgctttcgg tgtattcccg gggaccccgg gcgtgcagcg
     2281 cgaagacacg ttcaacgggt tgcgccttgc agcgggctac gccccgcgcc gcaatatccg
     2341 cgtttcgctc gcgtacgagc gcggcgatcg cgactccaac atcacgctgc gcgactacga
     2401 gtacaaccgc gtatccgcca acgcgcggtt ccagttctag acgcatcgaa ctccacccaa
     2461 ggacggcgtc gcttttcgcg gcgccgtttt tctttccggg gagggtcgcg gagcagagcg
     2521 cgcggtcaat cagtttctca cctccgcgat cacgccttgc tcgccgcccg cactgccgtc
     2581 catagacggc cgtggcggcc gcagcgccgc atccgcgggc ggcgcccacc cgatcggccg
     2641 gctcttctcc gtgcgaccgt acgcatcgtc gaagcgcacg atgtcgtctt ccccgagata
     2701 ggcgccggac tgcacctcga tgatttcgag cgcgaccttc ccgggattct cgaggcggtg
     2761 ccgcaccccg agcgggatgt aggtcgactg gttctccgtc agcaggaata cttcatcccc
     2821 gcgggtaatg cgcgccgtgc cgcgcacgac cacccagtgc tcggcgcggt gatggtgcat
     2881 ctgcaaagag agcgcggcgc ccggcttgac gatgatgcgt ttcacctgaa agcgctggcc
     2941 gctgtcgatg ccgtcgtagc tgccccacgg acgatagacc ttgcggtgat tgaggcactc
     3001 cttgcgcccg gcctccttga ggcgcgtcac cacctctttc acgtcctgcg ccttgtcctt
     3061 gcgtgccacc atgacggcat cgggcgtttc gatcaccacg atgtcgtcga gacccacgca
     3121 agcgatgaga cgcgattgcc cgatcagcag agaattgcgc gtatccaccg cacagacgtc
     3181 gccgtgcaat acattgccgc tgtcgtcctt cgaaccgatc tcccagagcg cgctccacgc
     3241 gccgacgtca gaccatccgg cctccattcg cacgaccgct gcgcggggtg ccgcagcgct
     3301 cgccggcgca ggctcgagcc gctccatcac cgcgtagtcg atcgagtccg acgcgcattc
     3361 ggcaaaacac ttgcggtcca cgcggaaaaa atcgctgtcc caggtaccga gcgcctgcgc
     3421 ccgctcgcac gctgctgcga tttccggccg atagcgcgtg atgctctcga gccagaccga
     3481 cgcgcgcacc gcgaagatgc cgctgttcca caggtatcgc cccgaggcgc agtaacgctt
     3541 cgcgttcgcc gtgtccggtt tctccacgaa cgaatggacc tggtatgcag cgggttcggc
     3601 actcgccgct atcttggctc cggtccgtat gtagccgtag ccggtctcgg ccgcgaccgg
     3661 cgggacgccg aaggtcacaa cgaagccctc cgcggcgagg gcggcgccca cgcctaaggc
     3721 cgcgcaaaat gccggctccg cggcgatgac gtgatcggac ggcatcacga gcagcacggg
     3781 atcctcaccg gcagcgttca gggcggcaag cgtgagtgcc ggcgcggtgc cgcgcccgac
     3841 cggttcgagc atgatggcgc gcgcgcgaac gtctatttcg cgcaactgct cagccaccag
     3901 aaaactgtgc tcctcgttac acaccacgat cggcgcagca acctcgcccg tgagccccat
     3961 cgctcgaagc gcagtcgcct gaagcagcgt ccgatcaccg ttcagcgcga gcagttgctt
     4021 gggatactgc tcgcgcgaga gcggccaaag gcgggtgccg gatcctccgc ataggaggac
     4081 aggctggatc ttcatcgagg atccccgaat gagtgagcaa cgagagtgga gactttatga
     4141 gcggcgcatg aaagctcttg cgccaccccg tgagccgtct aaccctcttt ttgctgggaa
     4201 ctacgcacga atcagtaatg aatgccgcgg aggatccgct ggaacgcggg ccgcggcgtg
     4261 ggaaaccata cggcgcgctt ctcgatgagg atccagtaag cccggatgag ggggtcggcc
     4321 ggatcgattc gtacattgtc cggctgcttc gtaagggatt gccctgtcgc agccgggcga
     4381 catccgcggt gtccgggccg at
//
LOCUS       DASURG010000386         5229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_168747, whole genome shotgun sequence.
ACCESSION   DASURG010000386 DASURG010000000
VERSION     DASURG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168747"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFY80_06770"
     CDS             complement(<1..1097)
                     /locus_tag="VFY80_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6007646.1"
                     /translation="MNAPIPRRTGAQLLVDALHIHGVDLAFGVPGESYLAVLDALYDK
                     RDVMRFVICRQEGGAANMAEAYGKMTGRPGICFVTRGPGACNASIGVHTAFQDSTPLF
                     LFVGQVSNEVAEREAFQEVDYRRMFGPLAKWVAQIDRADRVPEYISHAFHTAVSGRPG
                     PVVLALPEDMQTDVASVPDAGRYQRVAAHPGPTQMHQLRDLLAHAERPVVILGGSGWS
                     VQACEDFKSFVTTFNLPVACSFRAQDLLDNRDPHYVGEVAVGINPKLAERIRASDLLV
                     VIGARLGEWTTLNYSLVDIPRPRQRFVHIHPGAEELGRVYQGDLLINAGMPEFSAAAR
                     GLAPVRTLWDEWTEGARADFEAWQKPVAVPG"
     gene            1215..2207
                     /locus_tag="VFY80_06775"
     CDS             1215..2207
                     /locus_tag="VFY80_06775"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007647.1"
                     /translation="MAVLVTGGGGFVGLNLVEALLERGQEVILFDNGALPGPAQWALS
                     RYGRQLNIAPADVRDAAQVNQVFESHRIDYILHCAAMTSGAERERRDPATIVAINLQG
                     TLNVLDAARAYAVKRIVYTSSGAVYGESLYRLARLYEESPAVPVTLYGITKFAAERLC
                     LRMQALWEVDVVCARLGTVVGPWERDTGVRDSYGTHTQLAGLALSGGAAVLTPREVRR
                     DWVYSRDVAAGLIALMEAPRLRHRLYNLSAGVEWNHPIRTWCERLRSVYPDFDFRVAD
                     PGETANIWYTDQDRCPMDIGRISQDIGFVPRYTMDTAYSDFLEWLEGTPEMLRR"
     gene            complement(2643..3200)
                     /locus_tag="VFY80_06780"
     CDS             complement(2643..3200)
                     /locus_tag="VFY80_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX6007648.1"
                     /translation="MAKRSLFSLLSEQPWWVSLLVAMALFAIAQFVFPPVAPFVALPF
                     VAIAIWFAWQQMRNLSSATVETRLAAVRSMSWDEFSTRVMEAYRRRGYEVTPARSGSF
                     DFTLRRNGRVTLVQCRRWKVSQVGVGPVRELYEALDREEAFNAVCIAAGDFSEAARQF
                     ASGKPISLLYGTDLATLVGKVSASP"
     gene            3330..4526
                     /locus_tag="VFY80_06785"
     CDS             3330..4526
                     /locus_tag="VFY80_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6007649.1"
                     /translation="MSGTQPLKGIKVIELGTLIAGPFCTRLMAEFGAEVIKIEPPGGG
                     DPLRKWRKLHEGTSLWWYVQARNKKSVTLNLRLPEAQAIVRRLVKDADIVVENFRPGA
                     MEKWGLGWDALCAVNPGLVMVRLSGYGQTGPYRDKPGFGAIGESMGGLRYVTGYPDRP
                     PVRVGVSIGDSIAALHGVIGALMALHHRNRNGGRGQCVDVALYEAVFNMMESVLPEYG
                     MYGFVRERSGASLPGIVPSNTYITRDSKYVVIGANADAIFKRMMRAIGRNDLAEDPAL
                     AHNDGRVAHTDEIERVISDWVAQHDLEHVLETLDKAEVPSGKIYDIADIVQDAHYRAR
                     SMIEEHPLEDGTPLKVPGIVPRLSETPGETQWLGPKLGQHTAEILGTLGYSEQDQADL
                     HRRGVT"
     gene            complement(4841..4981)
                     /locus_tag="VFY80_06790"
     CDS             complement(4841..4981)
                     /locus_tag="VFY80_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX6007650.1"
                     /translation="MNITLSVGPLIALIAGILILIIPRLLNIIVALYLIIIGILGLFG
                     TR"
     gene            5059..>5229
                     /locus_tag="VFY80_06795"
     CDS             5059..>5229
                     /locus_tag="VFY80_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6007651.1"
                     /translation="MSSRPRPAVEPSLAPGVGRREVWAWAMYDFANSGYTTVVVTAVF
                     NTYFVAEIAGNAP"
BASE COUNT          951 a         1645 c         1733 g          900 t
ORIGIN      
        1 taacccggaa cggcgaccgg cttctgccag gcctcgaaat cagcgcgcgc gccctcggtc
       61 cactcgtccc aaagcgtgcg caccggtgcg agcccacgcg cggcggcgga aaactccggc
      121 atgccggcgt tgataagcag atcgccctga tacacccgtc ccaattcttc ggcgccaggg
      181 tggatgtgca cgaaacgctg gcgcgggcgc gggatgtcga cgaggctgta gttgagcgtg
      241 gtccactcgc cgagtcgcgc gccgatgacc acgaggagat cgctcgctcg gatgcgttcc
      301 gcgagcttcg ggttgatccc caccgccacc tcgccgacgt agtgcggatc gcggttgtcg
      361 aggaggtcct gcgcgcggaa tgaacatgcg acgggaagat tgaaggtcgt gacgaagctc
      421 ttgaagtcct cgcacgcctg cacactccat ccgctgccgc ccagaatgac aaccggtcgc
      481 tcggcgtgcg cgagcaggtc gcgcagttgg tgcatctgcg tcggacccgg atgggcggcg
      541 acgcgctggt agcgcccggc atccgggacg ctcgcgacgt cggtctgcat gtcctcgggc
      601 agcgcgagca cgaccgggcc gggtcgcccg gacaccgcgg tatggaacgc atgactgatg
      661 tactccggca cacggtccgc acgatcgatt tgcgcgaccc acttcgccag cggcccgaac
      721 atgcgccggt aatcgacctc ctgaaaggct tcgcgctccg cgacttcgtt gctcacctgc
      781 ccgacgaaga gaaagagcgg ggtggaatcc tggaaggcgg tgtgcacgcc gatcgatgcg
      841 ttgcacgcgc ccgggccgcg cgtgacgaag cagattccgg ggcggccggt cattttgccg
      901 taggcttcag ccatgttcgc cgcgccgcct tcctgccgac agatgacgaa gcgcatgacg
      961 tcgcgcttgt cgtagagcgc gtcgagcacg gcgagatacg actcgccggg cacgccgaaa
     1021 gcgagatcga cgccgtggat gtgaagagca tcgacgagaa gctgggcgcc ggtgcggcgc
     1081 ggaatgggcg cgttcatgtt ttcagcggca agagcagcgg gcaggaagac cgcgcaagcc
     1141 tatcatgaag ccctgcatgg cggcagccgg ccctgactta tactcccggc acttcccgaa
     1201 cgagcgaggc gcgtatggca gtgctggtga ccgggggcgg cggtttcgtc ggcctcaatc
     1261 tcgtcgaagc gttgctcgag cgagggcagg aggtgatcct cttcgacaac ggcgcgttgc
     1321 ccggacccgc ccagtgggcg ctgtcgcgct acggccgcca gctcaatatc gcgccggccg
     1381 acgtgcgcga cgccgcgcag gtgaatcagg tattcgagtc gcaccgcatc gattacatcc
     1441 tgcactgtgc tgcaatgacc tccggcgctg agcgcgagcg ccgcgacccg gcgaccatcg
     1501 tcgccatcaa tctgcagggc acgctcaacg ttctggatgc cgcacgcgcc tacgccgtga
     1561 aaaggatcgt ctataccagc tcgggcgcgg tgtacggtga atcgctctac cgtcttgcgc
     1621 gactctacga agagagcccc gccgtgccgg tcacgctcta cggaatcacc aagttcgctg
     1681 cggaacggct gtgcctgcgc atgcaggcgt tgtgggaggt cgatgtggtg tgcgcgcggc
     1741 tcggcacggt cgtcggcccc tgggagcgcg acaccggcgt gcgcgacagc tacggcacgc
     1801 atacgcaact cgccgggctg gcgctttccg gtggcgctgc ggtgcttact ccgcgcgagg
     1861 tgcgccgcga ctgggtgtac agccgcgacg tcgctgcggg attgatcgcg ctgatggagg
     1921 cgccgcgcct gcggcatcgc ctctacaatc tttccgccgg cgtggagtgg aaccatccca
     1981 tccgcacctg gtgcgagagg ctgagatccg tctatccgga tttcgatttt cgcgtcgctg
     2041 atcccggcga aacggcgaac atctggtaca ccgaccagga tcgctgcccg atggacatcg
     2101 gacgcatcag tcaggacatc ggttttgttc cgcgctacac gatggacacg gcatacagcg
     2161 attttctgga gtggctcgag ggcacgccgg agatgttgcg ccggtaggca cgcgtacaaa
     2221 aaaacccccg gcgctggccg ggggaaaaca ggggagggat ggaaccctgc ttggaggaaa
     2281 tttacagcgt gatgctgaac ccgtctgcgc taccgctggt gcaatctagc cgtaaggcaa
     2341 tgtttcgtcc gttgtccggg gtatagcagg cgccgtgcca gcagctaaat gcttggtgca
     2401 tcatgcactt aacaatttca ggccgtccgc acaagcgcga aaactgtcgc cggctgacga
     2461 cgttgtcatg agcgggcgcg ttatctggct gaccaattta gtttttttat gtcgtctgtc
     2521 gacgacaacg gcatatgagc gcgccagcct cccctacgtc atccacacgg gcacgccctt
     2581 gcggaaccgt tggaagcggg tgtacccata tgccgacggc atggcaaggt ctcaagacac
     2641 cgtcatggag aagcgctgac cttgccgacc aacgtggcga ggtcagtacc gtataggaga
     2701 ctgatcggct tgccggaggc gaactggcgg gcggcttccg aaaagtcgcc tgcggcgata
     2761 caaacggcgt tgaaggcctc ctcccggtcc agcgcttcgt agagttcgcg caccgggccg
     2821 acgcccacct ggctcacctt ccagcgccga cactgaacga gcgttacccg tccgttccgg
     2881 cgcaacgtga agtcgaatga cccgctgcgt gcgggcgtca cttcgtaccc gcgtcgccgg
     2941 taggcttcca ttacgcgcgt cgagaattca tcccaggaca tcgagcgcac tgcggccagg
     3001 cgcgtctcaa cggtcgcgga cgagaggttg cgcatctgct gccatgcgaa ccagatggcg
     3061 attgcgacga atggcagcgc gacaaacggc gcaaccggcg gaaagacgaa ctgcgcgatt
     3121 gcaaagagcg ccatcgcgac gagcagcgag acccaccatg gctgctcgga gaggagcgag
     3181 aacagggaac gtttggccat tggcaccgga tacggaacgc gcaatgataa gtcgggcgct
     3241 tcgtcccggc cagtgagtgg ccttcgggaa cgtgccgatc ggcgcgaacg gctctatact
     3301 gcgcttcccc gcggtctttt tttccagcga tgtctggcac acaacccctc aagggcatca
     3361 aagtcatcga gcttggcacg ctgatcgccg ggccgttctg cacgcggctc atggcggaat
     3421 tcggcgccga agtgatcaaa atcgagccgc cgggcggggg cgatccgctg cgcaaatggc
     3481 gcaaactcca cgagggcacg tcgctctggt ggtacgtcca ggcgcggaac aagaaatccg
     3541 tcaccctcaa cctgcgcctg ccggaagcgc aggcgatcgt gcgccggctc gtcaaggatg
     3601 ccgacatcgt cgtcgagaac ttccgccctg gcgcgatgga aaagtggggg ctcgggtggg
     3661 atgcgctctg cgccgtcaac ccgggcctcg tcatggtgcg gctctccggc tacggtcaga
     3721 ccggtccgta ccgcgacaag ccgggattcg gcgcgatcgg cgaatcgatg ggcggcttgc
     3781 gctacgtcac gggttatccc gatcgtccgc cggtgcgcgt gggcgtgtcg atcggcgact
     3841 cgatcgcggc gctgcacggc gtgatcggcg cgctgatggc gctccaccat cgcaaccgga
     3901 acggtgggcg cgggcagtgc gtcgacgttg ccctctacga ggcggtgttc aacatgatgg
     3961 agagcgtgct gccggagtac ggcatgtatg gctttgtccg tgagcggagc ggcgcgtccc
     4021 ttccgggcat cgtgccctcc aacacctaca tcacgcgcga cagcaagtat gtcgtgatcg
     4081 gtgcaaacgc ggacgccatt ttcaagcgca tgatgcgggc gatcggccgc aacgacctcg
     4141 cggaggatcc cgcgctcgcg cacaacgatg gccgcgtcgc gcacaccgac gaaatcgagc
     4201 gcgtgatcag cgactgggtc gcgcagcacg atctcgaaca tgtgctcgag acgctcgaca
     4261 aggcggaggt gccgagcggg aagatctacg acatcgccga tatcgtccag gacgcgcatt
     4321 accgggcgcg cagtatgatt gaggagcatc ccctggaaga cggcacgccg ctcaaggtgc
     4381 ccggcattgt gccgcggctt tctgaaacgc ccggggaaac gcaatggctg gggcccaagc
     4441 tcgggcagca taccgccgag atcctgggaa cgctcggcta cagcgagcag gatcaggccg
     4501 accttcaccg tcgcggtgtg acgtgagacg tgctgttaaa atcgcctcca attcgaacaa
     4561 ggaggcgccg atgatcatcc gatgcgtgcg cttcatgggg gctctggtcg tcgggctcgc
     4621 cgtgatggcg cctgcaggcg cacggtagcg tcggccatct cataccgcag ccgagccgtc
     4681 ttcgacatca aacggtgccg cgccaacccg atcagagcgc gctcgcgtgg cgttctcgta
     4741 ctcaccggat tcgcgctcgc gctcgatggg ctcgtgacgt tcgccgaacg cctctgctgg
     4801 tgtcgcggcc cgcccagagc gaaaccgggc cgctggagcg ctagcgcgtg ccgaagagtc
     4861 cgagtatgcc gatgatgatg agatagagcg cgacgatgat attgagaagc cgcgggatga
     4921 tcaggatcag tattcccgcg atcaatgcga tcagcggccc gacgctcaag gtgatgttca
     4981 tgccggtttc ctttcggacg ggcggctcgc ccgcgatgtc attggaatga cgaccgacgt
     5041 tcgacacggc tgcgcacggt gagttctcgg ccgcgccccg ctgtcgagcc gagcctcgcg
     5101 ccgggcgtag gcaggcgcga ggtatgggct tgggcgatgt acgacttcgc caattcgggc
     5161 tacaccaccg tcgtcgttac agcggtcttc aatacgtatt tcgtcgccga gatcgccggc
     5221 aatgcgccc
//
LOCUS       DASURG010000387         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_882911, whole genome shotgun sequence.
ACCESSION   DASURG010000387 DASURG010000000
VERSION     DASURG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882911"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFY80_06800"
     CDS             <1..284
                     /locus_tag="VFY80_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX6007652.1"
                     /translation="KTIGLRIPDHKVSQALLTELGEPLVTSTLLVPDQDAPLDDGQEI
                     RRHLERDVDLILDSGPCGIEPTTVVNLTGDTPVITRTGRGSIAPFAISA"
     gene            364..1062
                     /locus_tag="VFY80_06805"
     CDS             364..1062
                     /locus_tag="VFY80_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6007653.1"
                     /translation="MELNLVQTIAIYALPVIFAITLHEAAHGYVAKHFGDLTAYAEGR
                     VSLNPLRHIDLVGTVAVPLALLAISKLFAGSGILFGWAKPVPVNFANLRHPKRDMLWV
                     AAAGPFSNLAMALLWAFAVKIGISMPDNHFALPLALMGAAGVFINAIIMVLNLMPLPP
                     LDGGRILVSLLPHRLAYNVSRVEPYGFIILIVMLFSGVLGMILWPVIGTLLSLIAAMI
                     GLPVADLLRLANVL"
     gene            1086..2288
                     /locus_tag="VFY80_06810"
     CDS             1086..2288
                     /locus_tag="VFY80_06810"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX6007654.1"
                     /translation="MFVNRVLSGMRPTGSLHLGHHHGVLKNWITLQHEYECFFFVADW
                     HALTTHYDTPEIIEQNVWDMVIDWLAAGVDPGQATLFIQSRVPEHAELHLLLSMVTPL
                     GWLERIPTYRDQQEKLANKDLATYGFLGYPLLQSADILIYRANRVPVGEDQVPHIEFT
                     REIARRFNHIFGREAGFEQKAEAAIKKLGARRARLYRQLRSRYLEKGDEQALEAARAL
                     LEETQNLTLGDRERLFGYISGGGKMILSEPEALLTEASKMPGLDGQKMSKSYDNTIAL
                     REDPESVTKKIRTMPTDPARVKRSDPGEPEKCPVWQLHLVYSDEPRKQWVQQGCRTAG
                     IGCLDCKQPVIDAVLAELKPIRERAEEYLEDPTLVRNIIADGCEKAGKLAQETLRDVR
                     EVMGLNYT"
     gene            2458..>2676
                     /locus_tag="VFY80_06815"
     CDS             2458..>2676
                     /locus_tag="VFY80_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX6007655.1"
                     /translation="MSEPVNPLSASAPVARIYGQPLLEVPHDLYIPPEALEVFLEQFE
                     GPLDLLLYLIRKENINVLDIPMAQLTQQY"
BASE COUNT          524 a          846 c          803 g          503 t
ORIGIN      
        1 gcaagaccat cggcctgcgc attccagacc acaaggtatc gcaggcgttg ctgaccgagc
       61 tgggcgagcc gctggtcacg tccacgctgc ttgtgcccga ccaggatgcg ccgctcgacg
      121 acgggcagga gatccggcgt caccttgagc gtgatgtcga tctcattctc gacagcggtc
      181 catgcggaat cgaacccacg accgtcgtca atctgacagg cgatacgccg gtcatcaccc
      241 gcaccgggcg cggcagcatc gcccctttcg ccatctcggc atagggccgt cggagcctgc
      301 tagaatcggc gctcacggac acggtccgaa ccgcactgcg ccttccaaca agaacgcgac
      361 gccatggaat tgaacctcgt acagacgatc gcgatctacg cgcttcccgt gatctttgcg
      421 attaccctgc acgaagcggc tcatggctac gtcgccaaac acttcggcga cctcacggca
      481 tatgccgagg ggcgcgtgag cctgaatccg ctgcgccata tcgatctcgt aggcaccgta
      541 gcggttccgc tcgcgctgct ggcgatttca aagcttttcg cgggcagcgg aatcctcttc
      601 ggctgggcca agccggtgcc agtcaatttc gcgaatctgc gtcatcccaa gcgcgacatg
      661 ctgtgggtgg cggctgccgg gcccttcagc aatctcgcga tggcgttgct atgggcgttc
      721 gcggtgaaga tcggaatcag catgccggat aaccatttcg cgctgccgct cgccctgatg
      781 ggcgcggctg gcgtcttcat caatgccatt atcatggtgc tcaatctcat gccgctgccg
      841 ccgctcgatg gcggccgcat cctcgtgagt ctgctgccgc accgcctcgc ttataatgtc
      901 tcgcgcgtcg agccgtacgg tttcatcatt ttgatcgtga tgctgttttc gggcgtgctc
      961 ggcatgatac tgtggccggt gatcggcacg ctgctcagcc ttatcgctgc catgatcggc
     1021 ctgccggtgg ctgatctgct ccggctcgcg aacgtcctct agcgctaaga caacgatacc
     1081 caaaaatgtt tgtgaatcgc gttctgtcag gcatgcgccc gaccggcagc ctgcatctgg
     1141 ggcatcacca cggcgtgctc aagaactgga tcaccctgca acacgagtac gaatgcttct
     1201 ttttcgttgc cgactggcat gcactcacga cgcattacga cacgccggaa atcatcgagc
     1261 agaatgtatg ggacatggtg atcgactggc ttgccgcggg cgtggatccg gggcaggcga
     1321 cgctcttcat ccagtcgcgg gtcccggagc atgcggagtt gcacctgctg ctttccatgg
     1381 tgacgccgct cggatggctc gaacggattc ctacctaccg cgatcagcag gagaaactcg
     1441 ccaacaagga tctcgcgacc tacggatttc tcggctaccc gctgcttcaa agtgccgaca
     1501 tactgatcta tcgcgccaac cgcgtgccgg tcggtgagga ccaggtgccg catatcgagt
     1561 tcacccgtga gatcgcacgg cgcttcaatc acattttcgg cagagaggca ggattcgagc
     1621 agaaggccga ggcggcaatc aagaagctcg gcgcccgccg cgcccgactg taccggcaac
     1681 tgcgcagccg gtacctcgaa aaaggcgatg agcaggcgct ggaagcggcg cgcgcactgc
     1741 tggaagaaac gcagaatctg accctggggg accgggagcg gctgttcggc tacatcagcg
     1801 gaggtggcaa gatgatcctc tcggagcccg aggctctcct caccgaagca tcgaagatgc
     1861 ccgggctgga cgggcagaag atgtcgaagt cgtacgacaa cacgattgcg ttgcgcgagg
     1921 acccggagag cgttacgaaa aaaatacgga ccatgcctac ggatcccgca cgggtaaagc
     1981 gctccgaccc cggagagccc gagaagtgtc cggtatggca actgcacctg gtgtactctg
     2041 acgaaccgcg gaaacaatgg gtccagcagg gctgccgcac ggcgggcatc ggctgtcttg
     2101 actgcaagca gcccgtgatc gatgctgtgc tcgcggagct gaaaccgatc cgcgaacgcg
     2161 ccgaggagta cctcgaggac ccgacgctcg tgagaaacat catcgcggac ggttgcgaga
     2221 aagccggcaa gctcgcgcaa gagacgctgc gcgatgtacg cgaggtcatg ggcctcaact
     2281 atacgtgaag ctggttgccc gggcgttcgg tgcggcgtcg cgcgtcggct tttttctgta
     2341 gaatatcccg atgcagcccc tcacaatagg acgtgcccgc gaaggcgcgc gcaccgcacg
     2401 gtggatcgtc ctgttcaggg ccggtagccc ccccgacaca gcgatggggc agctcacgtg
     2461 agcgagccgg taaacccgtt gtcagcctcg gcgcctgtcg cccgcattta cggccagccg
     2521 ctgctcgaag ttccgcacga cctttacatc ccgcctgaag cgctcgaagt tttcctcgag
     2581 cagttcgaag gcccgctcga tctcctgctc tacctcatcc gcaaagagaa tatcaacgtt
     2641 ctcgacattc caatggcgca gctgacccag cagtac
//
LOCUS       DASURG010000388         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_168888, whole genome shotgun sequence.
ACCESSION   DASURG010000388 DASURG010000000
VERSION     DASURG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168888"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFY80_06820"
     CDS             <1..325
                     /locus_tag="VFY80_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007656.1"
                     /translation="GAFGVNLLSVTLDRRTFFHSDTLTSLQTPANSATMELLRSIEAL
                     LARGGVAEDLQGAGALHYLGRVIYAQAYTMGFRDSFLIVAIVFTLALIPAWIMGRAKP
                     LAETR"
     gene            complement(405..914)
                     /locus_tag="VFY80_06825"
     CDS             complement(405..914)
                     /locus_tag="VFY80_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6007657.1"
                     /translation="MLRAVAFAAHKHRDQRRKDKDASPYINHPIQLATVLWEEGGVHD
                     PDVIAAALLHDTLEDTETTWQELRGAFGEDIADVVLEVTDTKWIKKALRKRLQVARAR
                     HSSENAKLVKLADKICNLRDMSARPPAGWPLERKRQYFDWAKEVVDRLRGTHPELERR
                     FDEVYALKP"
     gene            1055..1414
                     /locus_tag="VFY80_06830"
     CDS             1055..1414
                     /locus_tag="VFY80_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX6007658.1"
                     /translation="MAHTSDTLERLRSALARFAAERDWDQFHNPKNLVMALAGEAGEL
                     VEHFQWLTFEQAVNLPRATRDEVALECADVLLFLLRLCDKLDIDLARAAQKKLALNAK
                     KYPVEKARGRATKYTKL"
     gene            1577..>2311
                     /gene="rlmJ"
                     /locus_tag="VFY80_06835"
     CDS             1577..>2311
                     /gene="rlmJ"
                     /locus_tag="VFY80_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX6007659.1"
                     /translation="MLAYRHLFHAGNFADVFKHALLTRLLIALGEKEKPYCYLDTHAG
                     IGRYDLTHAWAQKAREYENGIGRLWGRRDIPPVLAPYMEAVKEQNTGRKLRFYPGSPL
                     VAKRFMRPIDRIVLTELNKTDYAELKALFARDRRVGVHLLDGYQALKAFLPPKEKRGL
                     VLIDSSFDRSREFERIATALGAAHARWPTGIFAVWYPLMAPGSMRNFERDVKNLGVRK
                     ILKLEMMIAARDFDATIPGCGMLIVNP"
BASE COUNT          409 a          724 c          738 g          440 t
ORIGIN      
        1 cggcgcattc ggcgtgaacc tgctgtccgt cacactcgac cggcgcacgt tcttccacag
       61 cgatacgctg acgagcctgc agacgcctgc caacagcgcg acgatggagc tcttgcgctc
      121 gatcgaggcg ctgctggcgc gcggcggcgt cgcggaggac ctgcaaggcg cgggcgcgct
      181 gcattacctc gggcgcgtca tctacgctca ggcgtatacg atgggattcc gggacagctt
      241 cctgatcgtc gcgatcgtgt tcacgctcgc gctcattccg gcgtggatca tgggccgcgc
      301 gaaacctctg gccgagacga gataggcatt gccgttcgcc ctgatcggag cgacagcaga
      361 gttaaagggc cgttcatgct gcgacggcct caacacccgc ggagttaagg cttgagggcg
      421 tagacttcgt cgaagcggcg ctcgagctcg gggtgcgtgc cgcgaagccg atcgaccact
      481 tccttcgccc agtcgaagta ctgccgcttg cgctcgagcg gccagcccgc gggcgggcgc
      541 gcggacatgt cacgcagatt gcagatcttg tctgcgagct tcacgagctt cgcgttctcg
      601 cttgaatgcc gcgcgcgggc gacctgcagg cgtttgcgca gcgctttctt gatccacttg
      661 gtgtcggtga cttcgagcac gacatccgcg atatcctcac cgaacgcgcc gcgcagttcc
      721 tgccacgtcg tttcggtatc ttccagcgta tcgtggagga gcgctgccgc aatcacgtcc
      781 ggatcgtgca cgccgccctc ttcccacagc acggtcgcga gctgaatggg gtggttgatg
      841 taaggcgagg cgtccttgtc cttgcgccgc tggtcgcgat gcttgtgcgc agcgaaggcg
      901 accgcgcgca ggatgagatc ggcggactgc ctttcctgcc taacccgttt cgatggcgtg
      961 gcgactgcgg tatccacgtg cccgtgcttc acggtgtcgg aggaaggcca gtatacaatg
     1021 gcgcgctgca cgaggggtac cggagaacga tggcatggcg cacacaagcg atacgctgga
     1081 acggctgcgc agcgcgctcg cgcggttcgc ggccgagcgc gactgggatc agttccacaa
     1141 cccgaagaat ctcgtgatgg cgctcgcggg tgaagcgggc gagctggtgg agcatttcca
     1201 gtggctcacg ttcgagcagg cggtgaatct tccgcgggcc acgcgggacg aagtggcgct
     1261 cgagtgcgcc gacgtgctgc ttttcctgct gcgactctgc gacaagctcg atatcgatct
     1321 cgcgcgcgcg gcgcagaaga aacttgcgct caacgcgaaa aagtatccgg tcgagaaagc
     1381 ccgcggccgc gccaccaaat acacaaagct atagatatga gcctcttctg gacaccggct
     1441 tccgccggtg agacgaacac gcgctcgtca tgccggcgaa agcacggcgg agcgttcgtc
     1501 atcccggtga caaccggaat ccaggcaggc aggcgtcttt ttggatcccg gcgttcgccg
     1561 ggatgacgat ccattcatgc tcgcgtatcg ccatctcttc cacgccggca acttcgccga
     1621 tgtcttcaag catgcgctac tcacgcggct cctgatcgca cttggggaga aggagaagcc
     1681 ctactgctac ctcgacacgc atgcgggcat cgggcgctat gacctcacgc atgcgtgggc
     1741 gcaaaaggcg cgcgaatacg agaacgggat cgggcggttg tggggacgcc gcgatattcc
     1801 tcctgtgctt gccccttata tggaagcggt gaaggagcag aataccggcc gcaaactgcg
     1861 gttctatccc ggctcaccgc tcgttgcaaa gcggttcatg cgcccgatcg atcgcatcgt
     1921 gctgaccgag ctcaacaaga ccgattacgc cgagctcaag gcgcttttcg cgcgtgacag
     1981 gcgggtcggc gtgcatctcc tcgacgggta ccaggcgttg aaggccttcc ttccgccgaa
     2041 ggagaagcgc gggctcgtgc tcatcgattc gtccttcgat cgttcgcgtg aattcgagcg
     2101 tatcgccact gcactcggtg cagcgcacgc gcgttggccc accggcatct tcgccgtctg
     2161 gtatccgctc atggcgccag gctcgatgcg caatttcgag cgcgacgtga aaaacctcgg
     2221 cgtccgcaag attctcaagc tcgagatgat gatcgctgcg cgcgacttcg atgcgacgat
     2281 tcccggatgc ggcatgctga tcgtcaatcc g
//
LOCUS       DASURG010000389         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_883153, whole genome shotgun sequence.
ACCESSION   DASURG010000389 DASURG010000000
VERSION     DASURG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883153"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFY80_06840"
     CDS             complement(<1..404)
                     /locus_tag="VFY80_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6007660.1"
                     /translation="MTESVLHGVFPQPEKSLRDLVTSLLHQGFAKSVEPIASTTITTD
                     DKGLVAGEVRINTMTGAVPAYRAHPDKSGKFPVVLVVQEIFGVHEHIRDVCRRLAKLG
                     YFAITAELYYRQGDVTKFTDNQEIFAKVVNHVP"
     gene            539..1696
                     /locus_tag="VFY80_06845"
     CDS             539..1696
                     /locus_tag="VFY80_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6007661.1"
                     /translation="MKITSVKSYTVHPGWRKNLIFVKVETDEGIHGWGEAYSQYDRDR
                     PVVAQLEALGPYVVGRSPFDIKHFTQFAFDDYAARRGSVELYCAVSGIEQAMWDIVGK
                     ACGQPVYNLLGGRCRDKIRVYANGWSYGLKTPADYARAAEKVIAQGWTALKLDPLPAP
                     WRTYIPKEQEERAVAVVKAVREAVGRDVDILIDQHRRLAPMHAIRLDKRLAEFGLYWM
                     EESCQAEYPDELAQIRREIGVPMVIGEATYTKTGFRPLLERRAADILNPDVACCGGIL
                     ELKEIAAMAEPFLVAVSPHNYNSTVVALAATVHASATMPNFIITEYFLPFVEFGDRIS
                     PNQLKPKNGYIDLPTAPGLGVALDEKALVEVPGKRYELRKLRHPADEPGGI"
     gene            complement(1721..2146)
                     /locus_tag="VFY80_06850"
     CDS             complement(1721..2146)
                     /locus_tag="VFY80_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6007662.1"
                     /translation="MPNILVAVDGSKNAMRAIDYAVQHFGSAPVTLHLLTVEPPLDDY
                     GMVRAYLPKREHVKAMRARAQGLLEQAARRARGENITCTTHVAIGDVAGCIVDTARRL
                     KCSAIVMGTRGMGPLKGLILGSVASKVLHLAHVPVTLVK"
     gene            complement(2235..3275)
                     /locus_tag="VFY80_06855"
     CDS             complement(2235..3275)
                     /locus_tag="VFY80_06855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX6007663.1"
                     /translation="MRRQLIFTGDVNLMNVTDPAVPFSRVCDRLKDADLVFGNLECCL
                     YQPSAERSLMDEGFYVAPAIGQALVRAGYHAVGNANNVTYGADAIRSSLVELDRLGIA
                     HVGAGLNREAARAPAILEKGGMRFGFLQRTSVYWPTNHEAGEHSPGVATLRGHTAYQL
                     PLHKTRPEIPPANRPGVPPEILTWADPVYLAQYREEIAALRGKVDIVVASHHWGLHEA
                     VLQYMTEIAHAAIDAGADMVVGHGPHYSLPVEVYKGKPVFYGLGNFSFHTGHGGRKHG
                     DWVGMMARIALEERRIERVSFQFVRHNERNETLLRTMEEEPEMFALIAERSARFETRL
                     ESDGNSALVKLQ"
     gene            3484..3924
                     /locus_tag="VFY80_06860"
     CDS             3484..3924
                     /locus_tag="VFY80_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6007664.1"
                     /translation="MFKHILIPTDGSEQSQRAVRTGIELAKLHQGRVTGIHVIPDYHL
                     LIAYEGAFDPITEERIEEEARARADNYLAYVRKMAEESGVPCDTVCETSDHPYDAILK
                     TAEGKSCDLILMTSHGRKGLAAVLLGSETRKVLTHARVPVLVVR"
     gene            4447..>4606
                     /locus_tag="VFY80_06865"
     CDS             4447..>4606
                     /locus_tag="VFY80_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX6007665.1"
                     /translation="MKIHEYQAKEILRKYGVATPRGIPCFNVDEAVAAGSRLGGKVWV
                     VKAQIHAGG"
BASE COUNT          864 a         1442 c         1443 g          857 t
ORIGIN      
        1 ggcacgtggt tcaccacttt ggcgaagatt tcctggttgt cggtgaactt cgtgacgtcg
       61 ccctggcggt agtagagttc cgcggtgatc gcgaagtagc cgagcttcgc gagccggcgg
      121 catacgtccc tgatgtgctc gtgcacgccg aaaatctcct gcacgacgag caccacggga
      181 aactttccgc ttttgtcggg atgcgcgcgg taggccggca ccgcgcccgt catcgtattg
      241 atcctgactt caccggcgac gaggcccttg tcgtcggtcg tgatcgtcgt cgaagcgatc
      301 ggctccacgc tcttcgcgaa tccctgatgc agcagcgacg tcaccagatc gcgcaggctt
      361 ttttcgggtt ggggaaacac gccgtgcaga acggattcag tcatgttgcg ctcctctcga
      421 tgggtccggt gggctggctc ttgttcagaa cggggcggca attataccgt gcgctcgcgc
      481 gcgcttgaca cgcggaaagg gcgcggcata gatttcaacg ctttcccgag ggggtgccgt
      541 gaaaatcacg agcgtcaaga gttacaccgt ccatcccggc tggcgcaaga atctgatctt
      601 cgtgaaagtc gaaaccgacg aggggatcca cggctggggc gaagcctact cgcagtacga
      661 tcgcgaccgt cccgtcgtcg cacagttgga ggcgctcggt ccctacgtcg tcggccgcag
      721 ccccttcgac atcaagcatt tcacccagtt tgccttcgac gactatgcgg cgcgccgcgg
      781 ctcggtcgag ctctactgcg ccgtctccgg catcgagcag gcgatgtggg acatcgtggg
      841 caaggcctgc gggcagccgg tctacaacct gctcggcggc cgctgccgcg acaagatccg
      901 cgtgtatgcg aacggctgga gctacgggct caagacgccg gccgactatg cacgcgcagc
      961 ggaaaaggtc atcgcgcagg gctggacggc gctcaagctc gaccccctgc ctgcaccgtg
     1021 gcgcacctac attccgaagg agcaggagga gcgcgcagtg gcggtcgtca aagccgtgcg
     1081 cgaagcggtg ggacgcgacg tcgatatcct gatcgaccag catcgcaggc tcgcaccgat
     1141 gcacgcgata cgactggaca agcggctcgc ggaattcggg ctctactgga tggaggaatc
     1201 gtgccaggcg gagtatccgg acgagcttgc gcagatccgc cgcgagatcg gcgtgccgat
     1261 ggtgatcggc gaggcgacct ataccaagac gggattccgt ccgctgctgg agaggcgcgc
     1321 cgccgacatc ctgaatccgg acgttgcgtg ctgcggcggc attctggaat tgaaagagat
     1381 cgccgcgatg gcggagccct tcctcgttgc ggtctccccg cacaactaca attcgaccgt
     1441 cgtcgcgctt gccgccacgg tgcatgcgtc cgcgacgatg cccaatttca tcatcacgga
     1501 atatttcctg cctttcgtgg aattcggcga tcgcatctcg ccgaaccagc tcaagcccaa
     1561 gaacggctac atcgaccttc ccactgcgcc ggggctcggt gtggcgctcg acgagaaagc
     1621 gctcgtggaa gtgcccggga aacgctacga gttgcgcaag ctgcgccatc cggcggacga
     1681 gcccggcggc atctgatctg cagcggacct ggcgcgagcg ctacttcacc agtgtcaccg
     1741 ggacgtgcgc gaggtgcagc actttactcg cgacggaacc caggatgaga cccttgagcg
     1801 gccccatgcc gcgcgtgccc atgacaatcg cgctgcactt gagacgccgc gccgtatcca
     1861 cgatgcagcc cgccacgtcc ccgattgcga cgtgcgtagt gcaggtgatg ttttctccgc
     1921 gggcgcgtct ggcggcctgt tcgagcagac cttgggctcg cgcccgcatg gctttgacat
     1981 gctcgcgctt gggcagatag gcgcggacca ttccgtaatc gtccagcggc ggctcgaccg
     2041 tgaggagatg cagcgtgacc ggcgcactgc cgaagtgctg gacggcatag tcgatggcgc
     2101 gcatcgcatt cttcgagcca tccacggcga caaggatatt cggcatccga ctgtgctcct
     2161 ttccagcttg catcgtgccc gtagccacct tcagcggtat tgatcaggat caatagcagg
     2221 tgcgccgtct tgcatcactg cagcttcacg agggccgagt tgccatccga ctcgagacgt
     2281 gtttcgaagc gcgcgctgcg ctcggcgatc agggcgaaca tctcgggttc ctcttccatc
     2341 gtgcgaagca acgtctcgtt gcgctcgttg tgccgcacga actggaagga gacgcgctcg
     2401 atgcggcgct cctccagcgc gatgcgcgcc atcatgccga cccaatcgcc atgcttcctc
     2461 ccgccgtgcc cggtatgaaa tgaaaaattg ccgaggccgt agaagacggg tttgcccttg
     2521 tacacctcga ccggaaggga atagtgcggc ccgtggccga cgaccatgtc cgccccggcg
     2581 tcgatcgcgg catgggcgat ctccgtcatg tactgcagca ccgcctcgtg cagcccccag
     2641 tggtgcgagg cgacgacgat gtccaccttt ccgcgcagcg ccgcgatctc ctcgcgatac
     2701 tgcgcgagat aaaccggatc cgcccacgtc aggatttcgg gcggcacgcc ggggcggttt
     2761 gccggcggaa tttcagggcg cgttttgtgc agcggcaact ggtaggccgt gtgcccgcgc
     2821 agggtggcga cgcccggcga gtgttcgccg gcttcgtgat tggtgggcca gtacaccgaa
     2881 gtgcgctgaa gaaaaccgaa ccgcatgccg cccttctcga ggatcgcagg cgcgcgggcg
     2941 gcctcgcggt tgagcccggc gccgacatgc gcaatgccga ggcggtcgag ctcaacgagc
     3001 gacgagcgga tcgcgtccgc tccgtacgtc acgttgtttg cattgccgac ggcatgatac
     3061 cccgcgcgca ccagcgcctg cccgatggcc ggggccacgt aaaagccctc gtccatcaac
     3121 gaacgctctg cggacggctg gtagaggcag cactcgagat tgccgaatac caagtccgcg
     3181 tcctttagcc gatcgcatac ccgcgaaaac ggtacggcag ggtccgtcac gttcatcaga
     3241 ttgacgtccc cggtaaatat cagctgtcgg cgcatgaggg cgatccttgg actcgagaag
     3301 ccggcgcgct cttttcggcg gccgcgcggt gtgcgcgcta ttctaagagc gcccgtgggg
     3361 tggcgcatcc aaaatagtgc ttgacacccc atggcaggaa cgacaagctt tgccccgggt
     3421 ccaaataatg gaaaaaaggg tccgttcggg agcgcgcggc gactcccgcg gagaggaggc
     3481 tcgatgttca agcacatcct gataccgacc gacggttcgg agcaatccca gcgggccgtg
     3541 cgcacgggaa tcgagctggc gaagctgcac cagggacggg ttacgggcat acacgtcatt
     3601 ccggactacc acctcctcat cgcttatgag ggggcgttcg atcccatcac ggaagagcgc
     3661 atcgaagagg aagcccgggc gcgggcggac aactatctgg catacgtccg caagatggcg
     3721 gaagagtcgg gagttccttg cgacacggta tgcgagacga gcgatcaccc ctacgacgcc
     3781 atcctcaaga ccgcggaggg caagagctgc gatctcatcc tgatgacttc gcacgggcgc
     3841 aaaggccttg ccgcggtgct gctcggcagc gagacccgta aggtcctcac ccacgcccgc
     3901 gtgcctgttt tggtcgttcg ttaaccgtaa cccctccctc gtagagtccc gttttcggat
     3961 tattttttct aagctctcga ttctaaataa ttttagtgca aagcagcaaa aacaccttgc
     4021 ttggcgaccc gcgaagtggc acaatggcgc cgtgttcgat gctcgctcga gtgtcgggca
     4081 cactcctcca aaacctcctc ctttggtgga actctgcgca acccctcggg gttgcgcttt
     4141 ttttacccac gtggtaagta tgtgctcact atttgtttgt gctgcgaagc aatacaaaga
     4201 accgcacgag cggcaggccc ggtgcgcaac gaatagaaaa tcggattgaa tgccgctccg
     4261 acgctacacc gggcgaagag cgatcgaaag cgcctgcgca ctcgtgcgct tcccgttcgg
     4321 attgcatgcg cgctgtgcgc gttcggcgat acttgactgc ggtcaaggct tgggcgcgac
     4381 actcccgata aattcgagct tcttcaagtt ggcgcgccac cagtccactc cagaacgtct
     4441 tcctgaatga agatccacga atatcaggcc aaggaaatcc tgaggaaata cggcgtcgct
     4501 acgccgcgcg gcatcccatg cttcaacgtc gacgaggccg tcgcagccgg cagcaggttg
     4561 ggcggcaagg tgtgggtggt caaggcgcag atccatgccg gaggcc
//
LOCUS       DASURG010000390         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_67227, whole genome shotgun sequence.
ACCESSION   DASURG010000390 DASURG010000000
VERSION     DASURG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67227"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1000
                     /locus_tag="VFY80_06870"
     CDS             38..1000
                     /locus_tag="VFY80_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007666.1"
                     /translation="MKCIASTIAVALALAGAAPAAAQSYPAKPVRVVVPSGAGGSVDT
                     LARLVAQKMSASMGQQFVVENRSGSGGVIGTEIVARAAPDGYTLLMAYGSHVVNPTLY
                     PKLPYDTVKDFTPITQVAVQPLLVNVHPALPAKSIKELVALAKARPGQLNYGSAGAGS
                     GGHLATEILCMMAKVRMTHVPYKGSAAAMFDVVAGNTQLMVVTLITSLPQVRAGKLRA
                     IGITSAKRSPIVPDVPAVAETIPGYDVEVSYFFLAPTGTPRDIVTKLHGEATRALKQP
                     DVVERLARDGASPVANTPEETAQVIDREIVKWGEAVKASGAKER"
     gene            complement(1002..2000)
                     /locus_tag="VFY80_06875"
     CDS             complement(1002..2000)
                     /locus_tag="VFY80_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6007667.1"
                     /translation="MSQDPLSDLLGAVRLRGAVFYYVSFRQQWAAESPPAREIAEAVM
                     PGAEHVMAYHMVAKGNGWIAISGEQPVKLAAGDIVMLPQGDAHVMSSAPGMKYDRYDA
                     DWVFATRNVPKPMPISYHHGVLHPGVSIPVADAETILVCGFLGCDLRPFNPLVAALPR
                     LLHLPAHRAGAWIGRVIDQAALESAEPRPGAQAVLERLSEMMFVDTARRYLECLPDNA
                     TGWLAGLRDRYVGRSLTLLHTQPGRAWTIEELGREVGLSRSALHERFVQYLGQPPIQY
                     LANWRVQLGSKLLRETHRPVAAIALEVGYESEAAFSRAFKRLVGAPPAAWRRAQAN"
     gene            2176..>2738
                     /locus_tag="VFY80_06880"
     CDS             2176..>2738
                     /locus_tag="VFY80_06880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6007668.1"
                     /translation="MPATQICITPQSASVQPTPDFAAIKQRQQATWASGDYAVIGTTL
                     QMVGELLAEAVDLRAGERVLDVAAGSGNATLAAARRFATVTSTDYVAHLLEKGAARAR
                     AEGVEVAFQVADAEALPFDKASFDVALSTFGVMFTPDHAKAASELARVVRSGGRIGLA
                     SWTPDGFLGALFAVIGRFVPPPAGLRSP"
BASE COUNT          509 a          943 c          868 g          418 t
ORIGIN      
        1 cgactagatc cgcaagccca agcgaggaga acggaatatg aaatgcatcg ccagcaccat
       61 cgcggtcgcg ctcgcgctcg cgggcgccgc ccccgcggct gcgcagagct acccggccaa
      121 gcccgtacgg gtcgtcgttc cgagcggcgc ggggggcagc gtcgataccc tcgcccgctt
      181 ggtcgcccag aagatgtccg ccagcatggg gcagcagttc gtcgtggaga accgttccgg
      241 ctcgggcggt gtgatcggca ccgagatcgt ggcgcgggca gcgccggacg gctacaccct
      301 cctcatggcc tacggcagcc acgtcgtcaa tcccacgctg tatccgaagc tgccgtacga
      361 cacggtcaag gatttcacgc ccatcacaca ggtggcggtc cagccgctgc tcgtgaacgt
      421 ccatcccgcg ctgccggcga aatccatcaa ggagctggtg gcgctcgcca aggcgcgccc
      481 gggacagctc aattacggtt ctgccggcgc cggcagcggt ggccacctcg ccaccgaaat
      541 cctgtgcatg atggcaaaag tcaggatgac ccacgtgccg tacaagggtt cagcggccgc
      601 aatgttcgat gtcgtcgcag gcaacacgca gctcatggta gtgaccctca tcacgtcgct
      661 gccgcaggtg cgcgccggca agctgcgagc gatcggcata accagtgcga agcgctcccc
      721 catcgtaccg gacgtgcccg cggtagcgga gacgattccc ggctacgacg tcgaagtctc
      781 gtacttcttc ctcgcaccga ccggcacgcc gcgcgacatc gtcacgaaac tccacggcga
      841 ggcgacgcgc gcgctcaagc agcccgacgt ggtcgagcgt ctcgcgcgcg acggcgcctc
      901 cccggttgca aatacgccgg aagaaaccgc gcaagtgatc gatcgcgaga tcgtgaaatg
      961 gggcgaggcc gtaaaggcgt cgggagcgaa ggagcggtga gtcagttggc ctgtgcgcgc
     1021 cgccacgccg caggcggcgc accgacgagg cgcttgaacg cacgcgagaa cgccgcctcc
     1081 gactcgtagc ccacctcgag cgcgatcgcc gccacggggc ggtgcgtctc gcgcagcagc
     1141 ttcgagccga gctgtacccg ccagtttgcg agatattgaa tcggcggctg gccgagatac
     1201 tgcacgaagc gttcgtgcag tgcggagcgc gacaacccga cttcgcgccc cagctcttcg
     1261 atcgtccacg cgcgtcccgg ctgtgtgtgc agcagcgtca aagaacggcc cacgtaacgg
     1321 tcgcgcaagc ccgcgagcca cccggtcgca ttatcgggca ggcattccag atagcgccgc
     1381 gcggtgtcca cgaacatcat ctccgagagc cgctcgagca ccgcctgcgc gccgggccgc
     1441 ggctccgccg actcgagtgc cgcttggtcg atcacgcgtc cgatccacgc gcccgcgcgg
     1501 tgagcgggca gatgcagcag gcgcggcaat gcggcgacca gcggattgaa tggccgcaga
     1561 tcgcatccca gaaatccgca gaccagtatc gtttccgcat ccgcgaccgg gatcgatacg
     1621 cccggatgca gcacgccgtg gtgatatgaa atcggcatcg gcttcggcac attgcgcgtc
     1681 gcaaacaccc agtcggcatc gtagcggtcg tacttcatgc ccggcgcgct cgacatcacg
     1741 tgcgcatcac cctgaggcag catgacgatg tcgccggccg caagcttgac cggctgctcg
     1801 cccgatatgg cgatccagcc gttacccttc gcgaccatgt ggtacgccat gacatgctcc
     1861 gcgcccggca tcacagcctc cgcaatttcg cgcgcgggcg gggattccgc ggcccactgc
     1921 tgacgaaagc tcacgtagta gaacactgcg ccgcgcaggc gtaccgcacc gagaaggtct
     1981 gacagcgggt cttgcgacat gggcgcagcg gtttcgatgg agtcccgatc aagttatccg
     2041 gacgcccggc aaattcatca cacaaacgcg gacgcgtgag caaatgtgcc cgacgcgccg
     2101 tgaagcgtca atagaaaggc gtcccgacaa tacgcacgtc gcataagcga tactttcaac
     2161 cacaggagac gagaaatgcc cgcaacccaa atctgtatca caccgcaaag cgcatccgtt
     2221 cagcccacgc ccgatttcgc ggcaatcaag caacgccagc aggcgacatg ggcgagcggc
     2281 gactatgccg tcatcggcac gacgctgcag atggtcggcg agttgctcgc cgaagccgta
     2341 gacctgcgcg ccggtgaacg cgtgctcgac gtggcagccg gcagcggcaa cgcaacgctc
     2401 gccgccgccc gccgcttcgc gaccgtcact tcgactgact acgtagccca cctgctggag
     2461 aagggcgctg cgcgcgcccg tgcagaaggc gtcgaagtcg cgttccaggt cgccgatgcg
     2521 gaggcgctgc ctttcgataa ggcaagcttc gacgtggcgc tttcgacttt cggcgtcatg
     2581 ttcacgccgg atcatgcgaa ggccgcgagc gaacttgcgc gcgtcgtgcg ctccggcgga
     2641 cgcatcgggc tcgcatcctg gacccccgat ggctttctcg gagcgctttt cgcggtgatc
     2701 ggccggttcg tgccgccgcc cgcaggtctg cgctcccc
//
LOCUS       DASURG010000391         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_169817, whole genome shotgun sequence.
ACCESSION   DASURG010000391 DASURG010000000
VERSION     DASURG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169817"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFY80_06885"
     CDS             complement(<1..671)
                     /locus_tag="VFY80_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6007669.1"
                     /translation="MNKVGLVGWRGMVGSVLVQRMVAERDFDLIEVTFFSTSQVGGRG
                     PEIGRDAPAVKDARDIEALAKMEIIITCQGSDYTTEIYPRLRAAGWNGYWIDAAKTLR
                     MNEDAVIVLDPVNMHVIKQALARGVRNYIGGNCTVSCMLMGMHGLFAHDLVEWMTCMT
                     YQAASGGGAQHMRELLTQFGLIHEEVKALLADPASAILEIDRKVHAKQTDGALPMENF
                     GGVPLA"
     gene            complement(713..1777)
                     /gene="leuB"
                     /locus_tag="VFY80_06890"
     CDS             complement(713..1777)
                     /gene="leuB"
                     /locus_tag="VFY80_06890"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX6007670.1"
                     /translation="MKIAALAGDGIGPEIVVQALKVLRALARDGLGIEIEEAPFGGAG
                     YDAYGDPLPESTLKLAREADAVLCGAVGGPKYDALPRPQRPEQGILRIRKALGLFANL
                     RPALLYPELVGASTLKPEVVSGLDLMIVRELTGDIYFGEPRGRRQNASGAREGYDTML
                     YSEPEIVRIAEIGFQAALKRNRKLCSVDKENVLETSRLWREVVNDVGKKYPQVELSHM
                     YVDNAAMQLVRNPRQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDAHAKGLYEP
                     IHGSAPDIAGKNVANPLATILSTAMLLRYSFNQDEWAYRIEAAVKAVLAQGLRTADIH
                     EAGMHKIGTNEMGDAVVAAL"
     gene            complement(1785..2423)
                     /gene="leuD"
                     /locus_tag="VFY80_06895"
     CDS             complement(1785..2423)
                     /gene="leuD"
                     /locus_tag="VFY80_06895"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX6007671.1"
                     /translation="MEKFVRREGLVAPLDRANVDTDAIIPKQFLKSIERTGFGPNLFD
                     EWRYLDHGEPGMDHTKRPLNPDFVLNQPRYRGATVLLARENFGCGSSREHAPWALLQY
                     GFQAVIAPSFADIFYNNSLKNGLLLIKLDPKVVDRLFREVESAPVYRVAIDLEAQAIT
                     TPGGESFSFDIDPFTKHCLLNGLDEIGLTLQHADKIRAFEAQHRSRQPWLFG"
     gene            complement(2423..2548)
                     /locus_tag="VFY80_06900"
     CDS             complement(2423..2548)
                     /locus_tag="VFY80_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX6007672.1"
                     /translation="MKQVVILIWAMLSLTLAACNTIEGMGKDIRKAGESIEKAVK"
     gene            complement(2562..>3242)
                     /locus_tag="VFY80_06905"
     CDS             complement(2562..>3242)
                     /locus_tag="VFY80_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX6007673.1"
                     /translation="YVKGRPFAPAGALWDKAVAYWRTLKSDEDAKFDRVVHLKAEDIA
                     PQVTWGTNPQMVTTIDGRVPDPAQAQDAVQREWIERALKYMDLKPNTPITDIRLDKVF
                     IGSCTNSRIEDLRIAAQVVKGRRVAGNIKLAMVVPGSGLVKEQAEKEGLDRVFTEAGF
                     EWRQPGCSMCLGMNEDQLSSGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIVGH
                     FVDVRKFN"
BASE COUNT          556 a         1066 c          982 g          638 t
ORIGIN      
        1 gcgagcggca cgcccccgaa gttctccata ggcagcgcgc cgtcggtctg cttcgcgtgc
       61 accttgcgat cgatttcgag tatcgcggaa gcgggatccg cgaggagcgc cttcacctct
      121 tcgtgaatca gcccgaactg cgtcagcagt tcgcgcatgt gttgcgcgcc gcccccggac
      181 gccgcctggt acgtcatgca cgtcatccac tcgaccaggt cgtgcgcgaa cagcccgtgc
      241 atgcccatca gcatgcagct cacggtgcag ttgccgccga tgtagttgcg gacgccgcgc
      301 gcgagtgcct gcttgatgac gtgcatgttg accggatcga gaacgatgac ggcatcttcg
      361 ttcatccgca gcgtcttcgc tgcatcgatc cagtagccgt tccagccggc tgcgcgaagc
      421 cgcggataga tctcggtggt gtagtcgctt ccctggcacg tgatgatgat ctccatcttc
      481 gccagagcct caatgtcgcg cgcgtccttg accgcaggcg catcgcgccc gatctcgggg
      541 ccgcgcccgc cgacctgcga ggtcgagaag aacgtgacct cgatcaggtc gaagtcgcgc
      601 tcggcgacca tgcgctgcac gagcaccgac cccaccattc cccgccaacc gacgagacca
      661 accttgttca tgaccgccta ccgtatatcg accgggccat tttatcccaa ggctacagcg
      721 ccgcgaccac ggcgtcgccc atctcgttcg taccgatctt gtgcataccc gcctcatgga
      781 tgtccgccgt gcgtaagcct tgcgccagca cggctttcac cgcggcttcg atgcggtacg
      841 cccattcgtc ctgattaaag ctgtagcgga gcaacattgc ggtggaaaga atggtcgcaa
      901 ggggattggc gacgtttttt cccgcgatat cgggcgcgga cccgtggatc ggctcgtaga
      961 ggccttttgc atgcgcatcg agcgatgccg agggcagcat gccgatagag ccggtcagca
     1021 tcgatgcttc gtcggacagg atgtcgccga acatgttgcc ggtcacgatg acatcgaact
     1081 gcctgggatt gcgcacgagc tgcatcgccg cgttgtccac gtacatgtgc gaaagttcga
     1141 cctgcggata tttttttccc acatcgttca ccacttcccg ccacagccgg ctcgtctcga
     1201 gaacgttctc cttgtccacc gagcagagct tccgattgcg cttcaacgct gcctggaagc
     1261 cgatttccgc gatgcggacg atttccggct cggagtagag catggtgtcg tagccttcgc
     1321 gcgccccgct cgcgttctgc cgccgcccgc gcggctcgcc gaagtagata tcgcccgtga
     1381 gctcacgaac gatcatgagg tcgagccccg aaacgacttc cggcttgagc gtggacgcgc
     1441 ccacgagttc gggatagagc agcgcggggc gcaggttggc aaaaagcccg agtgccttgc
     1501 gaatgcgcag gatgccctgt tcgggccgct gcgggcgggg cagcgcgtcg tacttcggtc
     1561 caccgaccgc gccgcacagc accgcatccg cctcgcgcgc gagcttgagc gtggactccg
     1621 gcagcgggtc accataggcg tcgtatccgg ccccgccaaa cggcgcctcc tcgatttcga
     1681 ttccgagtcc gtcgcgcgcg agggcgcgca gcaccttcag cgcctggacg acgatctccg
     1741 ggccgatgcc gtcaccggcg agcgcggcga tcttcacgat cgggtcagcc gaagagccat
     1801 ggctggcgac tgcgatgctg tgcctcgaac gcacggatct tgtcggcgtg ctgcagcgtg
     1861 agtccgatct cgtcgagccc gttgagcaga cagtgcttgg tgaagggatc gatgtcgaag
     1921 gaaaagcttt caccgcccgg tgtcgtgatg gcctgcgctt cgaggtcgat agcaacgcgg
     1981 tataccggtg ccgactcgac ttcacgaaag agccgatcga cgaccttcgg atcgagcttg
     2041 atgaggagca agccgttctt cagcgaattg ttgtagaaga tatcggcgaa gctcggcgcg
     2101 atcaccgcct ggaagccgta ctgcagcagc gcccacggcg cgtgctcccg cgaagagcca
     2161 cagccgaagt tctcgcgcgc gaggagcacc gtcgcgccgc gataacgcgg ctggttcagc
     2221 acgaaatcgg ggttgagcgg gcgcttggtg tgatccatcc ccggctcgcc atgatcgagg
     2281 tagcgccact cgtcgaagag attggggccg aatcccgtgc gctcgatcga cttcaggaat
     2341 tgcttcggga tgatcgcgtc ggtatcgacg ttggcgcggt cgagcggcgc aaccaatcct
     2401 tccctgcgta cgaatttctc catcacttga ccgccttttc gatcgactcc ccggccttgc
     2461 ggatgtcctt gcccatgccc tcgatggtat tacacgcggc gagggtcaac gaaagcatcg
     2521 cccatatcag tattacaacc tgcttcattt tcggcctccc gtcagttgaa cttcctgaca
     2581 tcgacgaaat gccctacgat cgccgcggcc gccgccatcg ccgggctcac cagatgcgtc
     2641 ctgccgcccg caccctggcg cccttcgaaa ttgcggttcg acgtggaggc gcagcgctcc
     2701 ccggacgaaa gctggtcctc gttcatgccg aggcacatcg aacagccggg ctggcgccat
     2761 tcgaagccgg cttcggtgaa cacgcggtct agcccctcct tctcggcctg ctctttcacg
     2821 agaccggatc caggcacgac catcgcgagc ttgatgttgc ccgccacccg cctgcccttc
     2881 acgacctgag cggcgatgcg caagtcctcg atgcgcgagt tggtgcagga accgatgaag
     2941 accttgtcga gccgtatgtc ggtgatcggc gtgttgggct tcaagtccat gtacttgagc
     3001 gcgcgctcga tccattcccg ctgtaccgca tcctgagcct gtgcgggatc gggtaccctg
     3061 ccatcgattg tggtgaccat ctgcgggttc gttccccatg tgacctgcgg tgcgatgtcc
     3121 tccgctttga gatggacgac gcggtcgaac ttcgcgtctt catcgctctt gagcgtgcgc
     3181 caatacgcga cggccttgtc ccacagcgcg ccggccggag cgaacgggcg ccctttcacg
     3241 ta
//
LOCUS       DASURG010000392         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_883996, whole genome shotgun sequence.
ACCESSION   DASURG010000392 DASURG010000000
VERSION     DASURG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883996"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFY80_06910"
     CDS             complement(<1..698)
                     /locus_tag="VFY80_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6007674.1"
                     /translation="MSVPALGLIVVAAFIHAYWNLLVKRADGGPAFVWLYSLTSAVIY
                     APAVALIIVLDPPRYDALQWLIILVSGILHLLYAITLSHGYRVADLSVVYPLARGTGP
                     LFACVFAIVILGERPSALGLAGIALIIAGIFLIAASSLTSLIATGSRHRGFTYGVLTG
                     LCIASYTVNDAYAVKFLLVSPILLDYFGNLARLVFLTPSVVTARETASEQWRRNARLG
                     IAVGALVPLSYILAL"
     gene            923..1573
                     /locus_tag="VFY80_06915"
     CDS             923..1573
                     /locus_tag="VFY80_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007675.1"
                     /translation="MSSELLTSPLFVAAAAFAALGALFVLGGLVALLRARPLRFMLRT
                     LFGLLMLAVGALAGGIAVGIQGYQALTHETTAARISVSPIAPQRFTATFRFPNGRQDT
                     FELAGDALYVDAHILKWKPLANMVGLHTAYELDRIAGRYHAIERERSAPRTIYTVGRD
                     QPIDLFSLRRRYALLSIFLDADYGSASFVPVTRPEELELRVSTTGLLIRSAAPAAQ"
     gene            1759..2628
                     /locus_tag="VFY80_06920"
     CDS             1759..2628
                     /locus_tag="VFY80_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007676.1"
                     /translation="MHGFATGSAIDVMSRPLAAKLSEQLGQQFLVDARPGATGVIANE
                     LVAKAAPDGYTALAAPGSSLTSTPHLRKVPFDPLKDFAPVGLTTTFSFFMVAHPAVPA
                     KTSGDLVALGRKRTDLSFGSTGVGSAFHLAGELFSQMAGIPLLHVPYRGGGTGAITDL
                     IAGRVDLMFESPAVVAPYVRDGKLKAIGVTGTTRNALLPDVPTISEGGLKGYALTGWH
                     GMLLPAATPRPIVQRLNSALNQALQSPDVKALWAKMGMETPSTTPEQFAALMRSEFER
                     YGKLTASLGLKAN"
     gene            complement(2625..>3269)
                     /locus_tag="VFY80_06925"
     CDS             complement(2625..>3269)
                     /locus_tag="VFY80_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6007677.1"
                     /translation="HGVVGQGGERTEVRPGHCRLMPRGSEHFFFNETKEEEGLVIGFY
                     VGAKSVADTGYEFRGNVTEADLRMPRREKFSDGILVHIDDVKPTRMKEKEGWSITDFR
                     MPIGRHNGSSTTLFWAKFMPGAIHKKHRHENCEEIYYVIRGRGIAGAGRDRAEVRGGH
                     AHYIPKGVEHFLYNTSDTEPLEVIGIYIGAGSVEETGYVYTGEVTTSDLKPMSD"
BASE COUNT          564 a         1068 c         1016 g          621 t
ORIGIN      
        1 agcgcgagga tgtacgaaag cgggacgagt gcaccgacag cgatgccaag acgagcattc
       61 cgtcgccatt gttcactcgc cgtctccctc gccgtaacca cggagggggt gagaaagacg
      121 agccttgcca gattgccgaa atagtcgagc agtatgggag acaccagcag aaatttcacg
      181 gcgtacgcgt cgtttaccgt atagctggcg atacagagac cggtgagtac gccatatgtg
      241 aagccacggt gccggctgcc ggttgcgatc aacgaggtga gcgatgaagc ggcaatgagg
      301 aaaatccccg cgatgatgag cgcgattccc gcaagcccga gcgcgctcgg ccgctcgccc
      361 aggatgacga tcgcgaagac gcaggcgaat agcggacccg tcccgcgcgc gagcgggtag
      421 acgacggaga gatcagccac ccggtagccg tggcttagcg ttatcgcgta gagcagatga
      481 aggatcccgc tcacgaggat gatgagccac tgcagcgcgt cgtatcgcgg cggatccaat
      541 acgatgatca gcgcgaccgc cggcgcatag atgaccgccg aagtcaggga gtacagccag
      601 acgaacgccg ggccgccgtc cgcgcgcttg acgagcaggt tccagtaagc gtgaatgaag
      661 gccgcgacga cgatgaggcc gagagcaggg acggacatga tgcctccaga ggttgagccg
      721 aagcctcaac atctccgcct ctggggtttt gtccctcggg tgcaggccgg cttccccgcc
      781 ggctttccgt gacgctcggt ccaggcgcga aggcgtcgcc tcgccggaac cctagtcacc
      841 actcagttga tggtgcgact atagggatcg aaggttcggc tgtaaagttg cttccacagg
      901 caatcgatgg agtgatttag gtgtgtcaag cgagctgctg acttctccgc tcttcgttgc
      961 ggccgctgcc ttcgcggccc tcggcgcact cttcgtcctc gggggactcg tcgcgctact
     1021 gcgggcgcgc ccgttgcgct tcatgttgcg cacgctattc ggactgctga tgctcgccgt
     1081 gggcgccctg gcgggtggca tcgccgtcgg cattcagggt taccaggcat tgacgcacga
     1141 gacgaccgca gcgcgcataa gcgtgtcgcc cattgcaccg cagcgcttca cggccacttt
     1201 ccgctttccc aatggcaggc aagacacgtt cgagctggcg ggtgacgcgc tttacgtcga
     1261 cgcgcacatc ctgaagtgga agccgctcgc caacatggtg gggctgcaca ctgcgtatga
     1321 attggatcgc atagcggggc gctatcatgc catcgaacgt gaacgcagcg cgccgcgcac
     1381 gatctacacg gtgggtcgcg atcagccgat cgacctcttc agcctgcggc gccgttatgc
     1441 gctgctttcc atttttcttg atgcagacta cggctcggca tcgttcgtcc cggtcacgcg
     1501 cccggaagag ctcgagctgc gcgtatccac gacgggtctc ctcatccgga gcgccgcgcc
     1561 ggccgcacag tgaatgagct acactcgcgc cacggcgctg gctgatatgc gaggtaccca
     1621 atgacccacc ttttgcgtcg acctgactca tgccgcccgg tcacgacgtt gttgcggctt
     1681 tttttgctcg ccgctttagc aacctgcgtc gtcctgcctg cctcggcaca gcagtggcct
     1741 acgcgcagcg tcaggctgat gcacggcttc gcgaccggct cggcgatcga tgtcatgtcc
     1801 cggccgctcg cggcgaagct ttccgagcag ctcggccagc agtttctggt tgacgcgcgt
     1861 cccggagcga ccggcgtgat tgcgaacgaa ctcgtggcga aagccgcgcc cgacggctac
     1921 acggccctcg ccgcgccggg atcgtcgctc accagcacgc cgcacttgcg caaggtgccg
     1981 ttcgatccgc tgaaggattt tgcgccggtc ggcctcacca cgaccttctc gtttttcatg
     2041 gttgcgcacc cggcggtacc cgccaagacc tcaggcgatc tcgtggccct tgggcgcaag
     2101 cgcactgacc tctctttcgg ctcaaccggc gtcggcagcg cgtttcacct cgccggcgag
     2161 ctcttctccc agatggccgg cattccgctc ctgcacgtgc cgtaccgcgg aggcgggacc
     2221 ggtgcgatta ccgacctcat cgcgggccgc gtcgatctga tgttcgaaag tccggcagtc
     2281 gtcgcgccgt atgtgcggga tggaaagttg aaggcgatcg gcgtgaccgg cacgacacgc
     2341 aacgcgctgc tgcccgatgt gccgacgatc agcgaaggtg ggctcaaggg ctacgcgctc
     2401 accggctggc acggcatgct tctgcccgcc gccacaccgc gcccgatcgt gcagcgcctc
     2461 aatagcgcgc tcaaccaggc actgcagtcg cccgacgtca aggcgctgtg ggcaaagatg
     2521 ggcatggaga cgccctcgac cacgcccgag cagttcgccg cgctgatgcg ctcggagttc
     2581 gagcgttacg gcaagctcac cgcatcgctc ggtctcaagg cgaactagtc cgacatcggc
     2641 ttgaggtcgc ttgtggtcac ttcaccggtg tagacgtaac cggtctcctc gacgctgccc
     2701 gcgccgatgt agatcccgat tacctccagg ggctcggtgt cgctcgtgtt gtagaggaag
     2761 tgctcgaccc ctttcgggat gtaatgcgcg tgcccgccgc gcacttcggc gcgatcgcgt
     2821 ccggcgcccg cgatgccgcg tccccggatc acgtagtaga tctcttcgca gttctcgtgc
     2881 cggtgtttct tgtgtatcgc cccgggcatg aacttcgccc agaagagcgt cgtggaactg
     2941 ccgttgtgac gcccgatcgg catgcgaaaa tccgtgatgc tccagccttc cttctccttc
     3001 atgcgcgtcg gcttcacgtc atcgatgtga acgagaatgc cgtcgctgaa tttctcccgc
     3061 cgcggcatcc tcaagtccgc ttcggtgacg ttcccgcgaa attcgtagcc ggtgtccgct
     3121 acgctctttg cgccgacgta gaagccgatg acgagccctt cttcttcctt cgtttcgttg
     3181 aagaaaaaat gctccgagcc gcgcggcatc agccggcaat ggccgggacg cacttcggtc
     3241 cgttcgccgc cctgccccac cacgccgtg
//
LOCUS       DASURG010000393         5881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1089015, whole genome shotgun sequence.
ACCESSION   DASURG010000393 DASURG010000000
VERSION     DASURG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089015"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFY80_06930"
     CDS             <1..405
                     /locus_tag="VFY80_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX6007678.1"
                     /translation="EEPRVALLSFSTKASAQHARVDKVTRALAIVRERSPALAIDGEL
                     QADAALVPQVAAKKVKGESPVAGRANVLIFPDLDAGNIAYKLTQCLAGARAVGPVLQG
                     FAHPLSDLSRGASVEDIVATSAIVLALARAAA"
     gene            499..1473
                     /locus_tag="VFY80_06935"
     CDS             499..1473
                     /locus_tag="VFY80_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007679.1"
                     /translation="MRTAKPFVVVLFAVLAGTPAPHAGAQQEFPDKPIRLIIGSAPGS
                     GPDIMARLIGEHLDNVWGQRVVVDARPGVAGILSAEQALRANPDGYTWMMLTSQLFVA
                     TSVYPNLKFDLDQDFVSVSLVGLVPWVLTVNPQVPAKSVSALVALAKKSPGKIRYGSG
                     GPGSGEHFTTVMFTRLAGINMLHVPYKGVAQALLDTIANEIQMQFAVFPAAKPHVDSG
                     RLRGLGVSTAKRAPGLPDLPAIVDTVPGYVNFGWYSIVAPKGTPESVLAKASAEIVKA
                     AREPAFGERLKTLGIEIIAGGRKELDEFRAAERKRVTEIVKATGISIK"
     gene            complement(1517..2659)
                     /locus_tag="VFY80_06940"
     CDS             complement(1517..2659)
                     /locus_tag="VFY80_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX6007680.1"
                     /translation="MDEAQDKIEIHRGLKGVYFDRSRVCYIDGRAGELRYRGYSIHDL
                     AQHSTFEETCYLLLKGELPKRAELDAFTAKLKTARVLPQPIYEVIRTIRNAHPMDVLR
                     TAVSALAAFDPETADKSPEATLRKGIRLTSQVPMIVAAHEHIRNGREPVAPLGRLGHA
                     ANFLYMLKGKEPSDDAARLMDVDMVLHAEHGSNASSFAARVVAGTDADLHGAITAAVA
                     ALAGPAHGGAAENVMRMAQEIGDPSRTAEYVKTKRANKEPVMGFGHRVYRAEDPRARH
                     MRAGVEKLSREMGQPQWYRILEGVVEAMKPYARHGVNVNVDFYAGVVYFLNGIPEDLF
                     VPIFAVGRVPGWTVQVLEQLENNILLRPLTLYNGPPPRDYVPIDGR"
     gene            complement(2723..3907)
                     /locus_tag="VFY80_06945"
     CDS             complement(2723..3907)
                     /locus_tag="VFY80_06945"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX6007681.1"
                     /translation="MGGELLILNCGSSSIKFGVYTAAAEPRRLLHGTISGLGATPRFS
                     AVDEHGTPLPAGALTNDAGEPLTLHTAPRWLFDWLETGEAARNVRGAGHRVVHGGERF
                     LAPVRIDDEILDALERLDPMAPLHQPHNLAAARELRRLRPTLPQVACFDTEFHASQPA
                     LAQRYAVPRELTAQGIRRYGFHGLSYEYIASVLPDYLGTRAGGRIIVAHLGNGASMCA
                     MHDRRSVATTMGFTALEGLVMGTRSGAIDPGVIFYLVREKRMSVNEVEHMLYHGSGLL
                     GVSGVSSDMRELLASSDPHAREAVELFVYRAARELGSLAAALGGLDALVFTAGIGEHA
                     APVRAMLCEKARWLGIELDDEANARHARTISTPGSDVAVCVIPTNEEIVIARHTQRLL
                     CA"
     gene            complement(3911..5038)
                     /locus_tag="VFY80_06950"
     CDS             complement(3911..5038)
                     /locus_tag="VFY80_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6007682.1"
                     /translation="MKVLSGMRHAFATCELALVVSVAFFVSAPVLAMEQQKNVYATGA
                     DVRIETPVDGDLYAAAGKLHVAYPVRGDAVIAGGSVDLSASIGEDLRAAGGFISLSGK
                     IGGEALLAGGSIAFGPEAVVLGRTWVAGGDIAIAGTLESGLTVYGKNILVLGHIDGPV
                     ELYGEKIEIVASARIRGDVTYSSRSEIRIDPRAQITGTVTRARDTLEFPRPKLHLPGF
                     PVIRPLLLLGLLAAGALLLLLFPRFTAHALSTLDTSPLKSIGLGTAIFFSLPPVILLL
                     MLTIIGIPIALLLAALYAAALLVGYLITAFFIGDRLLHTVRRTPEARFGWRVLALLVA
                     LLLLWLAYALPYVGTFIALLALFAGLGAMVLQAFSRYAQTA"
     gene            5138..5458
                     /locus_tag="VFY80_06955"
     CDS             5138..5458
                     /locus_tag="VFY80_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6007683.1"
                     /translation="MPIYEYRCTACGFQHEYLQRMSDAPLTDCPECGKRAFNKLVSAA
                     GFQLKGGGWYVTDFKNNGTKSRKESDKKASDKKESDKPADSAKSSESKAEASCGTGAC
                     PACS"
     gene            5629..>5881
                     /locus_tag="VFY80_06960"
     CDS             5629..>5881
                     /locus_tag="VFY80_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007684.1"
                     /translation="MKRYFITGLLIWVPVVITLWVLNLLVSTMDQTLLLLPYTLRTES
                     WLGVHIPGLGVLLTLLVVFLTGVFAANMIGQRLVAFWEGL"
BASE COUNT         1052 a         1872 c         1941 g         1016 t
ORIGIN      
        1 gaagagccgc gggttgcgtt gctctcattt tccaccaaag ccagcgcgca gcacgcgcgc
       61 gtcgataaag tcacgcgggc gctcgcaatc gtgcgcgaac gttcaccggc gctcgcgatc
      121 gatggcgagc tgcaggccga tgctgcgctg gtcccgcagg tggcggcaaa gaaagtgaag
      181 ggcgagagtc cggtggccgg aagagccaac gtcctcattt tcccggatct cgatgcggga
      241 aacattgcct acaagctgac gcagtgcctc gccggcgcac gcgcggtggg tcccgtgctg
      301 cagggcttcg cgcacccctt gagcgatttg tcgcgcggtg cgagtgtcga ggatatcgtc
      361 gcgaccagcg caatcgtgct ggcattggct cgggcggccg cgtgaaccgg tattcgaggc
      421 gagatgctct cgcgcggagg ccgtgtagaa tgatgccgcc cgcataagaa aacaagagac
      481 aaccgtggag gagatgacat gcgtacggca aaaccgttcg tcgtggtact gtttgcggtg
      541 ctggccggga ctcccgcgcc gcacgcagga gcacagcagg aatttcccga caaaccgatc
      601 cggctgatca tcggcagtgc gccgggatcg gggcccgaca tcatggcgcg gctgatcggc
      661 gaacacctcg acaatgtgtg gggccagcgc gtggtggtcg atgctcgtcc cggggttgcg
      721 ggcattttga gcgcggaaca ggcattgcgg gcgaatcccg acggctacac gtggatgatg
      781 ctgacgtcac agcttttcgt cgccacgtct gtctatccca acctcaagtt cgacctcgac
      841 caggatttcg tctcggtctc gctggtcggc cttgtgccgt gggtgctgac ggtcaatccg
      901 caagtgccgg caaaatccgt ctccgcgctg gtggcgctcg cgaagaagtc gccgggcaag
      961 atccgatacg gctccggcgg cccgggcagc ggcgagcatt tcaccaccgt catgttcacg
     1021 cgtcttgcgg gcatcaacat gctccacgtg ccgtacaagg gcgtcgcgca ggcgctcctc
     1081 gacacgatcg cgaacgagat ccagatgcag ttcgcggtgt tcccggcagc caaaccgcac
     1141 gtcgattccg gaaggttacg cgggcttggc gtcagcacgg cgaagcgcgc ccctggcctg
     1201 cccgatctcc cggcgatcgt ggatacggtg ccgggttacg tgaacttcgg gtggtacagc
     1261 atcgtggcac ccaaggggac gcccgagtcg gtgctcgcca aggcaagcgc cgagatcgtc
     1321 aaggcggcgc gcgagccggc gttcggcgag cggctgaaaa cattgggcat cgagatcatc
     1381 gccggcgggc gcaaggaact cgacgagttt cgcgcagccg aacgcaagcg cgtgacggag
     1441 atcgtcaagg cgaccggcat ctccatcaag tagcggaggg cgcacccttc gatgcgcttg
     1501 atcgcctgac gtcttcttac cgtccgtcga ttggcacgta gtcgcgcggc ggcgggccgt
     1561 tgtagagcgt gagcggacgc agcaggatgt tgttctccag ctgctccagc acctgcacgg
     1621 tccatcccgg cacgcgtccc accgcgaaga tcggcacgaa gagatcctcc ggaatgccgt
     1681 tcaggaagta caccaccccg gcgtagaagt ccacgtttac gttgacgccg tggcgcgcgt
     1741 acggcttcat cgcttcgacc acgccttcga ggatgcggta ccactgcggc tggcccatct
     1801 cgcgcgagag cttttcgacg ccggcgcgca tgtggcgcgc gcgcggatcc tcggcgcggt
     1861 agacgcgatg cccgaagccc atgaccggct ccttgttcgc gcgttttgtc ttcacgtatt
     1921 cggccgttcg ggacggatcg ccgatctcct gtgccatgcg catcacgttc tccgccgcgc
     1981 cgccatgcgc ggggccggcg agcgcagcga cggccgcagt gatcgcgcca tgcagatccg
     2041 cgtccgtgcc cgcgacgacg cgcgcggcga aagaggaagc gttcgagccg tgctcggcat
     2101 ggagcaccat gtccacgtcc atcaggcgcg ccgcgtcgtc gctcggttcc ttgcccttca
     2161 gcatgtagag aaagttcgcc gcgtggccga gccgcccgag aggcgcgact ggctcgcgcc
     2221 cgttgcggat gtgctcgtgc gcggcgacga tcatcggcac ctgtgacgtg agacggatgc
     2281 ctttgcgcag cgttgcttcc ggcgacttgt cggcggtttc cgggtcgaac gccgcgagcg
     2341 cggacacggc ggtgcgcagc acatccatcg gatgggcgtt cctgattgtt cggatgactt
     2401 catagatggg ttgcggaagc acgcgcgcgg tcttgagctt cgcggtgaac gcgtcgagtt
     2461 cggcgcgctt cggcaactcg cccttcagca gcagatagca cgtctcctcg aaggtcgagt
     2521 gctgcgcgag atcgtgtatc gagtagccgc gatacctgag ctcgcccgcg cggccgtcga
     2581 tgtaacacac ccgcgaacgg tcgaagtaca cgcctttcaa gccgcggtga atttcgattt
     2641 tgtcctgggc ttcgtccatg gcgaattccc gtgatgatga gacgttattt tggttcgaaa
     2701 ccgctgcgcg gggaagcccg cttcaggcgc aaagcaggcg ctgggtgtgg cgcgcgatga
     2761 cgatttcctc gttcgtcggt atgacgcaca ccgcgacgtc gcttcccggg gtgctgatcg
     2821 tgcgcgcgtg gcgggcattg gcctcatcgt cgagctcgat tcccagccaa cgcgcctttt
     2881 cgcagagcat ggcacgcact ggtgcggcgt gctctccgat gccggcggtg aagacgaggg
     2941 cgtcgagccc cccgagcgct gccgcgagcg atcccaattc gcgcgcggcg cggtacacga
     3001 aaagttcgac ggcttcgcgc gcgtgcggat cggagcttgc cagcagctcg cgcatgtcgc
     3061 tgctcacgcc cgacacgccg agcaggccgg aaccatggta gagcatgtgc tccacttcgt
     3121 tcacgctcat gcgtttctcg cgcacaagat agaagatcac gccggggtcg atcgcgccgc
     3181 tgcgcgtgcc catgacgagg ccctcgagcg ccgtgaatcc catggtcgtc gccacgctgc
     3241 ggcgatcgtg catcgcgcac atgctcgcgc cgttgccgag atgggcgacg atgatccgcc
     3301 cgcccgcgcg cgtcccaagg tagtccggga gaacgctcgc gatgtactcg tacgacagtc
     3361 cgtggaatcc gtagcgacgg atgccctgag ccgtcagctc gcgcggcacc gcataacgct
     3421 gggcgagggc cggttgcgag gcatggaatt ccgtgtcgaa gcaggcgacc tgcggcaggg
     3481 tggggcgcaa cctcctcaat tcacgcgcgg ccgcgagatt gtgcggctgg tggagtggcg
     3541 ccatgggatc gagacgctcc agcgcgtcca ggatctcgtc gtcgatccgc acgggcgcga
     3601 gaaaccgctc gcccccgtgc accacccgat ggcctgcgcc acggacgttg cgcgcggcct
     3661 ctccggtctc cagccagtcg aagagccagc gcggcgcggt gtgcagagtg agcggctcgc
     3721 ctgcgtcatt cgtcagcgcg ccggcaggaa gcggagtgcc gtgctcatct accgcgctga
     3781 agcgcggcgt tgcgccgagg ccggaaatgg ttccatgcag gaggcgccgc ggctccgctg
     3841 cggcagtgta gacgccgaac ttgatgctgg aggagccaca gttgaggatc agcagttcac
     3901 cgcccatggg tcacgccgtc tgtgcgtagc ggctgaacgc ctgcagcacc atcgccccca
     3961 gtccggcaaa cagcgcgagg agcgcgatga acgtgccgac atagggaagc gcgtacgcga
     4021 gccacagaag caagagcgcg acgagcagtg ccaatacgcg ccagccgaac ctcgcttccg
     4081 gcgtgcggcg cacggtgtgc aacagccggt cgccaatgaa aaaggcagtg atcagatagc
     4141 cgaccagcag ggccgccgca tagagcgcgg cgaggagcag cgcgatcgga atgccgatga
     4201 ttgtcagcat gaggaggagt atgaccggcg gcaggctgaa aaagatcgcc gtcccgagcc
     4261 cgatgctctt gagcggcgag gtgtccagcg tcgagagcgc gtgtgcggta aaccgcggaa
     4321 agagtaagag caggagcgcg ccggccgcca gcaacccgag cagcagcaga gggcggatga
     4381 cgggaaatcc cggcaggtgc agcttcgggc gcggaaactc gagggtgtcc ctggcgcgcg
     4441 tgacggttcc ggtgatctga gcccgcggat cgatccgaat ctcgctgcgg ctcgagtagg
     4501 tgacatctcc gcggatacgc gcggaagcca cgatttcgat cttttccccg taaagctcga
     4561 ccgggccgtc gatgtggccg agcaccagga tgttcttgcc gtagacggtg aggccgcttt
     4621 ccagcgtgcc ggcgatcgcg atatcccctc cggcgaccca ggtgcgcccg agaacgaccg
     4681 cctccgggcc gaacgcgatg ctcccgcccg ccagcaacgc ctcgcctccg atctttccgc
     4741 tcaggctgat gaagccaccg gccgcacgca ggtcctcacc gatcgaagcg ctgaggtcca
     4801 ccgagccgcc cgcgatcacc gcgtcgccgc gaaccggata agccacatgc agcttgccgg
     4861 cggcggcgta gagatcaccg tccaccggcg tttcgattcg cacatcggcg cccgtggcat
     4921 agacgttctt ctgctgctcc atggccagga cgggtgcgga gacgaagaac gccacagaaa
     4981 cgacgagggc gagctcgcac gtggcaaaag catgccgcat gcccgaaagg accttcatgc
     5041 cttcctcgca ccttgtaatc cgggaaacca gccccaattt tcagtgcgat tgtaaccttt
     5101 tcaggtataa tgctttttcg ttttttgggg ggttgccatg ccaatatacg aataccggtg
     5161 caccgcctgc ggctttcagc atgagtactt gcagcggatg agcgacgcgc cgctcaccga
     5221 ctgcccggaa tgcggcaagc gcgcgttcaa caagctggtc agcgcggccg gctttcaatt
     5281 gaagggcggc ggttggtacg tcaccgactt caagaacaac ggcacgaaat ccaggaagga
     5341 atcggacaag aaggcatccg acaagaagga atccgacaag cccgcggaca gcgccaagtc
     5401 gtcggaatcc aaagcggaag caagttgcgg caccggcgct tgtcccgcgt gtagctgacg
     5461 ccaagccgcc agccggggcc ccggcgtggg ggtgcctgac gccaagccgg ggctgcgcac
     5521 aacagttcaa tatcccgcgc ggctccgcta tgatcgaatc gcggccgcac gctttaattt
     5581 ccttacggct gccgctcgat gcggccccac atgtcaagaa agactctgat caagcgctat
     5641 ttcatcaccg gtctcctgat ctgggtgccg gttgtgatca ccctgtgggt gctcaatctc
     5701 cttgtcagca ccatggacca gaccctgctg ctgctgccct acacgctgcg cacggaatca
     5761 tggctggggg tccatatccc cgggctcggg gtactgctga cactgcttgt cgtcttcctg
     5821 accggcgtct tcgccgccaa catgattggc cagcggctgg tcgctttctg ggaagggttg
     5881 c
//
LOCUS       DASURG010000394         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_682007, whole genome shotgun sequence.
ACCESSION   DASURG010000394 DASURG010000000
VERSION     DASURG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682007"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFY80_06965"
     CDS             complement(<1..210)
                     /locus_tag="VFY80_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007685.1"
                     /translation="MPLPYENLTPDRVLDAVESCGYRCDGRLLALNSFENRVYLVYLE
                     EGAPLVVKFYRPERSRSGSRSGTNRT"
     gene            complement(219..1319)
                     /locus_tag="VFY80_06970"
     CDS             complement(219..1319)
                     /locus_tag="VFY80_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007686.1"
                     /translation="MPHIPDPRRNASFAADDALGLLVQRSVTGDTAAAQALGRWACER
                     LQGERDDEIAQTLRQVPSPVAYRALWDAVCRAIETPDVAGGDGALTTRVFAFPILLVA
                     AARQPSRMPGALPDIGEVRDLLERHGAAGATRNFGLSNALCSVDTLERLKPSLVYRWS
                     REWSGPPRELAPADVHVSAGRERVHLRFLVGAGITPREAPSFLETASNIGAWGIPLTR
                     TLVRQLAQPTIELLAIPRAPVSLLRAPHAGRWAELEAAFNLFTSNTLRNFRSAVGDPV
                     VIVSAHRGEQECGEVRVSMSSVLDDTLLEGFRWPLHPLDDLESIAQVIAELMRDCRVQ
                     DVRFLDRVLPERLASGSLFLRGNDAIAPQAPH"
     gene            complement(1332..2291)
                     /locus_tag="VFY80_06975"
     CDS             complement(1332..2291)
                     /locus_tag="VFY80_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007687.1"
                     /translation="MKRVLLLLACAVAWPLAVGAAQTYPERPIRLIVGFPPGGAADIL
                     GRIAAQRLGDALGQQVVVDNRGGAGGLIATEIATKGAPDGYTLLFSSIPHVINPHLYR
                     KVSYDALQDFVPVIQFVSVPLMMAAAPALPVKSVKDLIALARSKPGEINYASAGSGSS
                     SHLAMELFKAMADVRMTHIPYKGTGPLITDLMTGQVSVTIASAVPLSPQVKAGRLRGL
                     AVTSPKRSAAFPELPAIAETVPGYEVINWFGIFAPRGTPKSIVTRLNAALNDALRSPE
                     LAKVLNARGADVVGGTPVAFARVVKADFAKWAKVVKESGARVD"
     gene            complement(2291..>3296)
                     /locus_tag="VFY80_06980"
     CDS             complement(2291..>3296)
                     /locus_tag="VFY80_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007688.1"
                     /translation="VKEGENVTASMFIRRSAATAILLGAAAGVAAAQGAANYPSKPIR
                     YIVGYTPGGTADMLARAVGQKLTEAWGHQVIVENRPGAGTNIGTEIAAKSPPDGYTLF
                     MPTVANAINPTLYPKLTYDPIRDFAYVMNFAKVPGILVVHPSVPVKNAKELVALARSR
                     PNELRHGSTGIGSPHHLAGEIFKTMAGVKMIHVPYKGATPAIADVVAGHIEVYFGAMV
                     STLPHAKSGRLRALGVTSTKRVAAAPDVATLDEQGLKGFETGSWFGMAVPTGTSRDII
                     MKLHAEAARAIAAPELRKRMSAEGAEFVGDTPEQFTAFLKGEIAKWGKAVKASGAKVD
                     "
BASE COUNT          553 a         1110 c         1084 g          549 t
ORIGIN      
        1 tgtgcgattc gttccggaac ggctccccga gcgactgcgc tcgggccggt agaacttcac
       61 caccagcggc gcgccctcct cgagatacac gagatagacg cggttctcga agctgttgag
      121 cgcgaggagc cgcccgtcgc agcgataacc gcagctctcc acggcatcca gcacgcgatc
      181 cggggtgagg ttctcgtaag gaagcggcat ggggaaacct agtgaggcgc ttgcggcgcg
      241 atcgcatcgt tgccgcgcaa gaacaggctt cccgacgcca gccgttcggg caacacgcgg
      301 tcgaggaagc gcacatcctg cacccggcag tcgcgcatga gctcggcaat gacctgcgcg
      361 atgctctcca ggtcgtcgag cggatgcaac ggccagcgga acccctcgag gagcgtgtcg
      421 tcgagcacgg aactcatgct gacgcggacc tcgccgcact cctgttcgcc ccggtgcgcg
      481 gagacgatga cgacagggtc gcccacggcg gagcggaagt ttcgcagcgt gttgctggta
      541 aaaagattga aggcggcctc gagttccgcc caccggccgg cgtgcggcgc acgcagcaga
      601 ctcaccggtg cgcgcggaat ggcgagcagc tcgatcgtcg gctgcgccag ctgtcgcacc
      661 agcgtccgcg tgagcgggat gccccatgcc ccgatgttgg acgcggtttc gagaaaagac
      721 ggcgcttcgc gcggcgtgat gccggcaccg accagaaagc gcagatgcac ccgctcgcgc
      781 cccgcactca cgtgcacgtc cgcaggcgcg agctcgcgcg gagggcccga ccactcgcgg
      841 ctccaccggt acacgaggct cggcttcagc cgctcaagcg tatcgaccga gcacagcgcg
      901 ttgctcaacc cgaagttgcg ggttgcgccc gcagccccgt gccgctccag caagtcacga
      961 acctcgccga tatcgggcaa cgcgccgggc atgcgtgagg gctggcgcgc cgccgcgacg
     1021 agcaggatcg ggaacgcgaa cacccgtgtg gtgagcgctc cgtcgccgcc cgccacatcg
     1081 ggcgtctcga tcgcacggca cacggcgtcc cacaaggcgc ggtaggcaac gggcgaaggc
     1141 acctgccgca gcgtctgcgc gatctcatcg tcgcgctcgc cttgaagccg ctcgcatgcc
     1201 cagcgcccga gcgcctgggc ggcggcagtg tcgcccgtta cgctccgttg gacgaggaga
     1261 cccagcgcat catcagcggc gaatgaggcg ttgcgcctcg ggtccggaat gtgcggcatg
     1321 ctgcgtcggt atcaatcgac ccgcgcgccc gattctttca cgaccttcgc ccactttgcg
     1381 aagtccgctt tcaccacccg cgcgaacgcc accggggtgc cgccgacgac atccgcgccg
     1441 cgcgcgttga gcaccttcgc gagctccggc gagcgcaacg cgtcgttgag cgcggcattg
     1501 aggcgcgtca cgatggactt cggtgtgccc cgaggcgcaa atatgccgaa ccagttgatg
     1561 acttcgtagc cgggaaccgt ttccgcaatg gccggcaact cgggaaaagc ggccgagcgc
     1621 ttggggctgg tgaccgcaag accgcgcagt ctcccggcct tcacctgagg agagagcgga
     1681 accgcgctcg cgatcgtcac gctgacctgt ccggtcatga ggtcggtgat caggggtccc
     1741 gtgcctttgt acggtatgtg cgtcatccgt acgtccgcca tggccttgaa gagttccatc
     1801 gcgagatggc tcgacgatcc gctgccggcc gaagcgtaat tgatctcgcc gggcttggac
     1861 cgtgcgagcg caatgaggtc cttgaccgat ttcaccggca gcgcgggagc ggctgccatc
     1921 atgagcggca cgctgacaaa ctggatcacg ggaacgaaat cctgcagggc gtcgtaactc
     1981 accttcctgt aaagatgcgg gttgatgacg tgcggaatgg aggaaaagag cagcgtgtag
     2041 ccgtccggag cacctttcgt cgcgatctcg gtggcgatta gccctcctgc gccgccgcga
     2101 ttgtcgacga ccacctgctg ccccagcgca tcgcccagcc gttgcgccgc aatgcgcccg
     2161 aggatatcgg ccgcgccgcc cggtggaaag ccgacgatca gccggatcgg gcgctccgga
     2221 taggtctgcg ccgcgcccac cgcaagcggc cacgcgaccg cgcatgccag gaggaggagt
     2281 actcgtttca tcaatccacc tttgcgcccg atgccttgac ggccttgccc cacttcgcga
     2341 tttcgccctt gaggaacgcc gtgaactgct ccggcgtgtc gccgacgaat tccgcgccct
     2401 cggcgctcat gcgcttgcgc aattccggcg cggctatggc gcgtgcggct tccgcgtgca
     2461 gcttcatgat gatgtcgcgc gacgtgcccg tgggcaccgc catcccgaac cacgagccgg
     2521 tctcgaaccc cttgagcccc tgctcgtcga gcgtggcgac gtccggcgcc gccgcgacgc
     2581 gtttcgtgct ggtcaccccg agggcacgca gccgcccgct tttggcatgg ggcagcgtcg
     2641 agaccatcgc gccgaaatac acctcgatat ggccggcgac gacatcggcg atcgcgggcg
     2701 tcgcaccttt gtaagggacg tgaatcattt tcactcccgc catggtcttg aagatctcgc
     2761 cggcgagatg gtgcgggctg ccgatgccgg tcgagccatg gcgcagctca ttcggtcgcg
     2821 acctggccag tgcgaccaat tccttggcgt tcttcacggg cacggaagga tgcaccacga
     2881 gtatccccgg caccttcgcg aagttcatga cgtaggcaaa atcccggatc ggatcgtacg
     2941 tgagcttggg atagagcgtg ggattgatgg cattcgccac tgtgggcatg aaaagcgtgt
     3001 agccgtcggg cggagacttg gcggcgatct cggtcccgat gttcgtgccg gcgcccggcc
     3061 gattctcgac gatgacctgg tggccccatg cctcggtcag tttctgcccc acggcgcgcg
     3121 ccagcatgtc ggcggtgccg cccggcgtgt agccgacgat gtagcgaatc ggcttcgacg
     3181 gatagttggc ggcgccttgg gccgccgcca ctccagccgc ggcgccgagg aggatggccg
     3241 ttgcggcact ccgccggatg aacatcgatg cagtcacgtt ctctccttct tttacg
//
LOCUS       DASURG010000395         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1500241, whole genome shotgun sequence.
ACCESSION   DASURG010000395 DASURG010000000
VERSION     DASURG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500241"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFY80_06985"
     CDS             <1..718
                     /locus_tag="VFY80_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6007689.1"
                     /translation="IDRPPLAEGGPVEVARAARAFNTMQSRLQRYIRDRTRILAAMSH
                     DLKTPITRLRLRSELLGDGELRTRFTRDLDEMESMVAATLDFLRGLESGEAVQPIDVT
                     ALLESLQADLAETGGVVRIEGRAARAYPGRPQALKRCLSNLLENAIKYGKTATVRVED
                     DATRLEISIADEGPGLPDAELERVFEPFYRTEQSRSRETGGTGLGLTIARSVAEIHGG
                     KIALRNRPEGGLEARVTLPR"
     gene            772..1479
                     /locus_tag="VFY80_06990"
     CDS             772..1479
                     /locus_tag="VFY80_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007690.1"
                     /translation="MPPRDRSSEWFVPAFGPPRFRLYVGLLFLPYTGMVLSYVVIGSM
                     LAETVHWDRVGAILVIYFVALGIGAHALDAIGGDGLKPWGEAFSRTQLWTLAAVAVVT
                     AYAIAIYFAVYYVPLLWLIAIPEGFFLFAYNLEWFRGRFHTDGWFAVSWGVLPVLAGY
                     VIQTNSVSLAALVVAAGAGCLSLVEIKASRPYKDMKRSGMDRNTPQVRTLEAILKSVS
                     AGVILLGIGLALWRAVG"
     gene            complement(1542..>2222)
                     /locus_tag="VFY80_06995"
     CDS             complement(1542..>2222)
                     /locus_tag="VFY80_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6007691.1"
                     /translation="DRLAIVHRFFSGTGPTYDFIVNAATFGIDRRWKRRILALLPEKP
                     KRIVDFACGTGILTVEIARRHPDCHVVGVELRDEYLQHAREKVRRLGLSNVELVLSRA
                     EDYLASETCDCVCSSYLAKDADLPLLTRNTHEMLADGGLLLMHDFIYPPSRAVQLLWK
                     VYFKSLQFTGTPLFPEWREIFYGLPQLIERTRWVEELHQALPANHFRDIRIEPMTLHG
                     SAIVTARK"
BASE COUNT          386 a          681 c          741 g          414 t
ORIGIN      
        1 catcgaccgg cctccgctcg cggagggcgg gcccgtcgag gtcgcgcgcg cggcccgcgc
       61 cttcaacacc atgcagtccc ggctgcagcg ctacatccgt gaccgcacgc gcatcctcgc
      121 cgccatgtcg cacgatctca aaacgccgat cacgcgcttg aggttgcgct cggagctgct
      181 cggcgacggc gagcttcgga cgcgcttcac gcgcgacttg gacgagatgg aatcgatggt
      241 cgccgcgacg ctcgatttcc tgcgcgggct cgagagcggt gaggcggtgc aaccgatcga
      301 tgtcactgcc ttgcttgaaa gcctgcaggc cgatctcgcg gagaccggcg gcgtcgtgcg
      361 catcgaaggg cgggcggcgc gcgcgtatcc cggccggccc caggcgctca agcgctgcct
      421 gagcaatctc ctcgaaaacg ccatcaaata cggaaagacc gcgacggtgc gcgtggagga
      481 cgacgcgacg cggctcgaga tcagcattgc ggacgaagga cccggcctgc ccgatgcgga
      541 actggagaga gtgttcgagc ccttctatcg caccgagcag tcgcgcagcc gcgaaacggg
      601 aggtacggga ctcggtctca cgatcgcgcg cagcgtcgcg gaaattcacg gcggcaagat
      661 cgcgcttcgc aatcgcccag aaggcggact cgaggcgcgc gtcacgctgc cgcggtagac
      721 taacccatcc atccctgacg ctgctccttc tgggggcaga aacgaccgtt gatgccgccg
      781 agagatcgca gcagcgagtg gttcgtgccc gcgttcgggc cgccgcgctt tcgcctgtac
      841 gtgggcctgc ttttcctgcc gtataccggc atggtgctgt cgtatgtggt gatcggctcg
      901 atgctggcgg agaccgtcca ctgggatcgc gtcggcgcca ttctcgtgat ttatttcgtc
      961 gcgctcggca tcggtgcgca tgcgctcgac gcgatcggtg gcgatgggct gaagccatgg
     1021 ggcgaagcgt tttcacgcac gcaactctgg acgctcgccg cagtcgcggt cgtgaccgca
     1081 tacgccatcg cgatctactt cgcggtgtac tacgtgccgc tcctgtggct gatcgcgata
     1141 cccgagggct tctttctctt cgcctacaac ctcgaatggt tcaggggacg gtttcacacc
     1201 gatgggtggt tcgccgtgtc ctggggcgtg cttccggtgc tcgccggata cgtcattcag
     1261 accaacagcg tgtcgcttgc agcgctcgtg gttgcggcgg gtgcgggttg cctcagcctc
     1321 gtcgagatca aggcctcgcg cccgtacaag gatatgaagc gatccggcat ggacaggaat
     1381 acgccgcagg ttcgcaccct ggaagcgata ctgaagagcg tgagcgccgg cgtgattctc
     1441 ctcggcatcg ggcttgcgct atggcgcgct gtcggctaag ggcggcattc gtccggtgaa
     1501 tacgggattc catgcgtccc gggtggcgga taaaggacag gctacttccg cgccgtcacg
     1561 atcgccgacc cgtgcagtgt cataggttct atcctgatat cgcggaagtg gtttgctggc
     1621 agcgcctggt gcagttcctc gacccagcgc gtcctttcga tcaactgtgg caggccgtag
     1681 aagatctcgc gccattctgg aaagagtggc gtgcccgtga attgcagcga cttgaagtag
     1741 accttccaca gaagctgcac ggcgcgcgac ggcggataga tgaagtcgtg catgaggaga
     1801 agtccgccgt ccgccagcat ctcatgagtg ttgcgtgtga gaagcggcag atccgcgtct
     1861 ttcgcgaggt acgacgagca cacgcagtcg catgtctcgc tcgcgaggta gtcttccgca
     1921 cggctcaaca ccagctcgac gttggagagc ccgaggcgcc gcaccttttc gcgggcgtgc
     1981 tgaaggtatt cgtcgcgcag ctcgacgccg acgacatgac aatcgggatg gcgccgcgcg
     2041 atctcaaccg tgagtattcc ggtgccgcaa gcgaaatcga cgatgcgttt gggcttttcg
     2101 ggaagaaggg cgaggatgcg gcgcttccac cggcgatcga tgccgaaggt cgcggcattg
     2161 acgatgaagt cgtaagtcgg gcctgtcccg gaaaagaagc ggtggacgat cgcgagtcga
     2221 tc
//
LOCUS       DASURG010000396         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_375742, whole genome shotgun sequence.
ACCESSION   DASURG010000396 DASURG010000000
VERSION     DASURG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375742"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFY80_07000"
     CDS             complement(<1..672)
                     /locus_tag="VFY80_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007692.1"
                     /translation="MDRSLNLARCLVAVALCSTAGWVSAAEPYPNKPIRMIVPFGAGG
                     STDVLIRIVANKLPEAVGQQVVVDNRTGAGGLIGTEIAAKSTPDGYTLLGTGTPHTIA
                     PNLYKKVPFHPLKDFEPIMQMASQPYGLVVHPSLGVKSVPELIALAKKEPGKHNYASS
                     GQGGAMHLFQALFVNMAGINMVHVPYKGSGPVRADLVGGQIKIGCLGLSSIMPHYKAK
                     QLNILA"
     gene            complement(736..1710)
                     /locus_tag="VFY80_07005"
     CDS             complement(736..1710)
                     /locus_tag="VFY80_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007693.1"
                     /translation="MKVAASLALALICASGSVALNEVQAQSYPSRAVRIVVPFSPGGA
                     ADTPGRMLAPRLAEFLGQQVLIDNRPGAGSTIGTELVAKSPPDGYTLLLISNTHAINA
                     SLYKKLNYDAVSDFAPVLQFANSPNVLVVHPSLPAKSVKELIALAKTKPGQIDYASSG
                     NGSSQHLFTALFTTMAGIEMNHVPYKGSGQARTDLVSGQISVSMPGIASVIQHVRTGR
                     LRALGVTSAKRSPQLPEVPTISEAGVKGYEADQWLGLIAPAKTPAEAISKLNDAAQKA
                     LNQPDLRKSFENVGAEAHYLGPKEFGDLVKSEMPKWAKVVKATALQIN"
     gene            1843..2532
                     /locus_tag="VFY80_07010"
     CDS             1843..2532
                     /locus_tag="VFY80_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6007694.1"
                     /translation="MLRIRGLSKSYGGVRPRILLREVALDLGMGEYLAIMGESGVGKS
                     TLLNLIAGLDLPDAGSIIVDGVEMTALDDARRTLLRRSRIGFVFQAFHLLPHLTVAQN
                     VALPLALNAAGAEATRIRVAQMLEAVGLAQAARAYPRELSGGEMQRVAIARALAHRPK
                     LLLADEPTGNLDAESAAQVIALLREQLKSERASGILVTHSARAAATTDGALVLTDERL
                     QAAPCRNRAYE"
BASE COUNT          465 a          851 c          822 g          518 t
ORIGIN      
        1 ggcgaggatg ttgagttgct tcgccttgta gtgcggcatg atgctcgaga gccctaggca
       61 tccgatcttg atctggccgc ccacgagatc ggcgcgaacc ggcccgctgc ccttgtacgg
      121 cacatggacc atgttgatgc ccgccatgtt gacgaatagc gcctggaaga ggtgcatcgc
      181 cccgccctgc cccgaggacg cgtaattgtg cttgccaggc tccttcttcg ccagggcgat
      241 gagttccggc accgatttga cgccgagcga cggatgcacc accagcccat agggctggct
      301 cgccatctgc atgatcggct cgaagtcctt caacgggtgg aacggcactt tcttgtaaag
      361 attcggcgct atggtatgcg gcgtgccggt accgagcagc gtgtagccgt cgggagtcga
      421 tttcgccgca atctcggttc caatcaaccc tcccgccccg gtacggttgt cgaccaccac
      481 ctgctgcccc accgcttcgg gcagcttgtt ggcgacgatc cgtatgagca cgtccgtcga
      541 gccgcctgca ccaaaaggaa cgatcatgcg aatcggcttg ttcggatatg gttccgctgc
      601 ggacacccat cctgcggtag aacagagggc gaccgcgacc aagcatcgcg cgagattgag
      661 agatcgatcc atgcttcctc ctcgttatga cagcgaccgc gatcatacca agggcccaag
      721 tcgggcaaaa gccggctaat tgatctgcag cgcggtcgcc ttgacgacct tggcccactt
      781 gggcatctcg gacttgacaa ggtctccgaa ttccttcggg ccgagatagt gcgcttccgc
      841 gccgacattc tcgaacgatt ttctgagatc gggctgattg agcgccttct gggccgcgtc
      901 gttcagcttt gagatcgctt ctgccggcgt tttcgcgggg gcgatcaacc cgagccactg
      961 atccgcctcg tatcctttga cgcccgcctc cgaaatcgtc ggcacttcgg gcaactgcgg
     1021 ggagcgcttc gcactggtca cgccgagcgc ccgcagccgg ccggtgcgga catgctgaat
     1081 cacgctcgca atgccgggca tgctcaccga gatttgcccg ctcaccagat cggtgcgcgc
     1141 ctggccgctc cccttgtacg gcacgtgatt catctctatt ccggccatcg tcgtgaacag
     1201 agcggtgaaa agatgctgcg agctgccgtt gccggaggat gcatagtcga tctgccccgg
     1261 cttcgtcttc gccagcgcaa tcagctcctt caccgacttg gccggcagcg agggatgcac
     1321 gaccagcacg ttcggtgaat tcgcaaactg cagaaccggc gcgaagtcgc tgaccgcatc
     1381 gtagttgagc ttcttgtaca gactggcatt gatcgcatgt gtattcgaga tgagaagcaa
     1441 cgtgtagcca tcgggaggcg acttcgcaac gagctcggtg ccgatcgtac tgcccgcccc
     1501 agggcgattg tcgatcagca cctgttggcc gaggaactcg gcgagcctcg gcgccagcat
     1561 gcgccccggc gtatccgccg ctccgccggg agaaaacggc acgacgatgc gaaccgcacg
     1621 actgggatag ctctgcgctt gaacttcgtt caacgcgacg ctgcccgacg cacagatgag
     1681 cgccaaggca agcgatgcgg caaccttcat ggtgttctcc tcttgagctt cgcgaaaaaa
     1741 acggtggatg gtagcagaat tcatcgtcac gggcgctcca ccgctcgctg cgttccgagc
     1801 gtgctaatat cagtcaactt cctgtaatgg ctggggaaga cgctgctacg catccgcggg
     1861 ctctccaaat cgtatggcgg tgtccggccg cgcatcctcc tgcgtgaagt tgcgctcgat
     1921 ctcggaatgg gcgagtacct cgcgatcatg ggtgaatcgg gggttggcaa gtcgacgctg
     1981 ctcaatctga ttgctgggct cgacctcccc gacgcgggca gcatcatcgt agatggcgtt
     2041 gaaatgacgg cgctcgatga cgcgcggcgc acgctcctgc gccgctcgcg catcggcttc
     2101 gtattccagg cgtttcatct cctcccacat cttacggttg cgcagaacgt tgcgttgccg
     2161 ctcgcgctga atgctgcagg cgcggaggct acgcgcatcc gcgtcgcgca gatgctcgaa
     2221 gcagtcgggc tcgcgcaagc ggcgcgggcc tatccgcgcg agctctccgg cggcgaaatg
     2281 cagcgcgttg ccatcgcgcg tgcgttggcg caccggccaa aactcctgct ggcggacgag
     2341 cctacgggta acctcgacgc tgaatcggct gcgcaggtca tcgcgctgct gcgcgagcag
     2401 ctcaaaagcg agcgcgcgag cggcatactg gtgacacatt cggcgcgggc cgccgcgacc
     2461 accgacggtg cgctcgtgct gacagacgag cgcctgcagg cggctccctg caggaatcgg
     2521 gcatacgaat gacgccggcg tttcgctttc cgcgcttcgc ggtgggctac gcgctcaccg
     2581 ttggtccgct gcgcgaacat cccgggcgcg ccgttctcgc ggccgccgcc atagcgttgg
     2641 gggtcgcact cggagt
//
LOCUS       DASURG010000397         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1193188, whole genome shotgun sequence.
ACCESSION   DASURG010000397 DASURG010000000
VERSION     DASURG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193188"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFY80_07015"
     CDS             complement(<1..364)
                     /locus_tag="VFY80_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007695.1"
                     /translation="MPMEWLIRHSAKSPNRAKLTWLALVAMAPVAALAGGSNYGITPG
                     AHPNLAGKVSEWPVPTPRFARDPAPAPDGSIYIAVMSGNRVARFDPKTQQFREWELPP
                     GHRPHGVLVDRQGIVWTTG"
     gene            complement(397..1203)
                     /locus_tag="VFY80_07020"
     CDS             complement(397..1203)
                     /locus_tag="VFY80_07020"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX6007696.1"
                     /translation="MPIPPPTVAAGAPLCERRDAGGVTTLTLNRPQQYNALSLALIEE
                     LEAGLDSIARDAAVRVVVLAGAGKAFCAGHDLKELRAHAGGDFAREAFERFARCMVAL
                     TRLPQPVVARVHGFAFAAGCQLVAQCDLAVASTEARFATSGVNFGLFCNTPGVALARN
                     VSRKVAMEMLLTGEAIDARTALARGLVNRVVPPDELDREIARLTGSILAKTPVAIAAG
                     KRAFYRQIEAGLEEAYAQATEAMVCNLMTEDAAEGIDAFVQKRPPQWKNR"
     gene            1371..2477
                     /locus_tag="VFY80_07025"
     CDS             1371..2477
                     /locus_tag="VFY80_07025"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX6007697.1"
                     /translation="MPTFRYGHASAPDWRDAARSILAQLGAGEASLGFLYITDVLADH
                     ASDILAWFREETGVPHWTGAVGIGVCATGREYLDEPAIAVMVADFEPGTFRIFSNISR
                     AEQVDRAALKCAKAPANFAVVHADPTNAEVPELVKRLAERVESGFLVGGLTSSRRQNI
                     QIADGVFEGGLSGVSFSDAVAVATRLTQGCSPIGPKHGITACQRNVIVSLDGQPALEV
                     FKQDIGEALARDLNRVGGHIFAGLPIPGSDTGDYLVRNLVGIDTAHNLVAIGELLEPG
                     GSIMFCRRDDGTAREDMTRMLESIRKGLYTQPRGGVYFSCLGRGASLFGPDSRELKMI
                     REALGDLPLVGFFCNGEISHNRLYGYTGVLTLFV"
     gene            2497..>2735
                     /locus_tag="VFY80_07030"
     CDS             2497..>2735
                     /locus_tag="VFY80_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HEX6007698.1"
                     /translation="MNGMRLFTELTVTWLDVFAFAFFLAAWTGYVRFATRRGTRVPSL
                     QTSMDGYRRTWMVRMIERDNRMVDVNIMRNLTRSS"
BASE COUNT          445 a          882 c          903 g          505 t
ORIGIN      
        1 ttcccgtcgt ccacacgatt ccctgcctgt cgacgagcac gccgtgcggg cggtgcccgg
       61 gcggcagctc ccattctctg aactgctggg tcttcggatc aaaacgcgcg accctgtttc
      121 cgctcatgac ggcgatgtag atgctgccgt cgggcgcggg tgcgggatcg cgcgcgaagc
      181 gaggcgtggg aaccggccac tcgctcacct tgccggcgag attgggatgc gcgcccggtg
      241 tgatgccgta attcgagccg ccggcaagcg cggcaacggg cgccatggcc acaagggcga
      301 gccaggtaag tttcgctcga ttcgggcttt tggcggaatg gcgaatcagc cactccatgg
      361 gcatgcacga gtctccattc atctgccgat ctccggtcac ctgtttttcc actgtggcgg
      421 gcgtttctgc acgaacgcat cgatgccctc tgcggcatct tccgtcatca ggttgcacac
      481 catggcctcg gtcgcctgcg catacgcttc ctcgaggccg gcctcgatct gccggtagaa
      541 cgcgcgcttg cccgccgcta tggcaacggg cgtctttgcc agtatcgatc cagtgagcct
      601 ggcgatttcc cgatcgagct catccggcgg cacgacccgg ttcacgagcc cgcgggccag
      661 cgcggtccgg gcgtcgatcg cctccccggt gagcagcatt tccatggcca ctttgcgcga
      721 aacgttgcgc gcgagcgcga cgccgggcgt attgcagaag agaccgaagt tgacgcccga
      781 ggttgcgaac cgggcttccg tcgaagcgac cgcaagatcg cactgggcta cgagctggca
      841 gcctgcggcg aatgcaaagc catggacgcg cgcaacgacg ggctgcggca gccgcgtgag
      901 cgcgaccatg caccgcgcga agcgctcgaa tgcttccctc gcgaagtcgc cgcccgcgtg
      961 cgcgcgcagc tccttgagat cgtgaccggc acagaacgct tttcccgctc cggcaaggac
     1021 aacgacgcgc acggcggcgt cgcgtgcgat gctgtcgagg ccggcttcca gctcctcgat
     1081 cagcgcgagg gacagcgcgt tgtattgctg tggacggttg agcgtgagcg tcgtgacgcc
     1141 gcccgcgtca cggcgctcgc aaagcggcgc gccggcggcg acagtcgggg gtgggatcgg
     1201 catgcatctc tccaccggga atgataggga gattatagcg gctgtttatg cggagataga
     1261 gaagcgcgga cgatcatcgt gctgttgcca ccggcatgcg cggcggccgc ggttggcgat
     1321 tgccgtgccg cgcgtgctac gatttgccgt ccatcatccg cagtcaggct atgcccacgt
     1381 tccgttacgg tcatgcaagc gcccccgatt ggcgcgacgc cgcgcgctcg attctcgccc
     1441 agctcggcgc gggggaagcg agcctcggct ttctttacat caccgatgtg ctcgcggatc
     1501 atgcgtcgga cattctcgcg tggttcaggg aagagaccgg cgttccgcac tggaccggag
     1561 cggtcggcat cggcgtttgc gcgaccggcc gcgagtatct cgacgaacct gccattgcgg
     1621 tcatggtcgc cgacttcgag ccgggaacct tccggatctt ttccaacatc tcccgcgcgg
     1681 aacaggtgga tcgtgcggcc ctcaagtgcg ccaaggcgcc ggccaatttc gcggtggttc
     1741 acgccgatcc cacgaacgca gaggttccgg agctggtgaa aaggcttgcc gaacgcgtcg
     1801 agagcggttt tctcgtcggc gggcttacca gctcgaggcg ccagaacatc cagattgccg
     1861 acggtgtttt cgaaggcggg ctttcgggcg tcagcttttc ggatgccgtg gctgtcgcga
     1921 cgcggcttac gcaaggctgc tcgcctatcg gcccgaaaca cggcatcacg gcgtgccagc
     1981 gcaacgtgat cgtcagcctc gacggccagc ccgcgctcga agtgttcaag caggacatcg
     2041 gggaggcgct cgcgcgcgat ttgaatcgcg tcggcgggca tatcttcgcc ggcttgccga
     2101 ttcccggcag cgataccggc gactacctcg tgcgcaatct cgtgggaatc gacaccgcgc
     2161 acaatctggt cgcgatcggc gagctgctcg agccgggggg cagcatcatg ttctgccgcc
     2221 gtgacgacgg caccgcgcgc gaggacatga cgcgcatgct ggagagcatc cgcaagggcc
     2281 tttacaccca gccgcgtgga ggcgtctatt tctcatgcct cggccggggg gcgagcctct
     2341 tcgggcccga ttcgcgcgag ctcaagatga tccgcgaggc cctcggcgat ctgccgctcg
     2401 tgggattctt ctgcaacggc gagatctcgc acaaccggct ctatggctat accggcgtgc
     2461 tgacgctgtt cgtatagaac aatacggagg gggcgcatga acggcatgcg gctgtttacc
     2521 gagctcacgg tcacgtggct cgatgtcttc gcgttcgctt ttttcctggc ggcatggacg
     2581 ggatacgtgc gcttcgccac gcgtcgcggg actcgcgttc ccagcctgca gacgagcatg
     2641 gacggctacc gccgcacgtg gatggtgcgc atgatcgagc gcgacaaccg catggtggat
     2701 gtcaacatca tgcgcaatct cacccgcagc tcgca
//
LOCUS       DASURG010000398         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1295470, whole genome shotgun sequence.
ACCESSION   DASURG010000398 DASURG010000000
VERSION     DASURG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295470"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..636)
                     /locus_tag="VFY80_07035"
     CDS             complement(148..636)
                     /locus_tag="VFY80_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX6007699.1"
                     /translation="MRLILVLAFLAGLTACATRTAEHRPAADWPGRSAQDGSPGSRIA
                     LRALDFVGVPYRYGGRSPDTGFDCSGLVHYVVANVTGMPLPRRAQDMSRVGAPVRRRE
                     LAPGDLVFFDTLREPYSHVGVYLGESRFVHAPSNGGRVEVVSMNERYWLRRYNGARRI
                     SN"
     gene            complement(736..1221)
                     /locus_tag="VFY80_07040"
     CDS             complement(736..1221)
                     /locus_tag="VFY80_07040"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX6007700.1"
                     /translation="MARSILIFVIGGLLGAAAGFALGIFVYPYIFLADIVATERLDAG
                     TSKRLVATGTFRHANPSDPIHYGKGQVKAFEDLVYLEADFEVGPGPKYHVYLVPERNV
                     TPSTDVARTMFIDLGRLKAFKGSQNYSVPHGVDVSRYGSVVIWCEQFGVLISPAALTP
                     G"
     gene            1449..>3197
                     /locus_tag="VFY80_07045"
     CDS             1449..>3197
                     /locus_tag="VFY80_07045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6007701.1"
                     /translation="MARAVADAIAESRILVVEAGTGTGKTFAYLVPALMSGGKVILST
                     GTKTLQDQLFDRDIPTVRRALGIPVTVALLKGRANYVCHYHLERALAEGRFQSRQEPQ
                     DLARIADFARSSVTGDKADCAVVPENAGAWSHATSTRDNCLGSECGHYERCFVMAARR
                     QALAADVVVVNHHLFFADLVLRDEGAGELLPACNTVIFDEAHQLPETASLFFGESVST
                     TQYAELARDARAEGAVAARECPELQRAAHALEKAARDLRLAFTEDNGRLPLRAVERNS
                     GFAPVLAAVLARLDELAHILAEQAQRSDGLERCWQRALSLAEQTERWRSAADGARVRW
                     VELFSRALHLHSTPLSIADRFHDQISAQPRAWIFTSATLSVGGNFDHYRSELGLEDGE
                     DRSWESPFDYERQALLYVPKGLPEPNTPEHTAAVVSAALPLIEACGGRTFVLCTSLRA
                     MREAYLLLHDAFRRRSLGYPLLAQGEGSRTELLERFRTLGNAVLVGSHSFWEGVDVRG
                     NALSLVVIDKLPFSAPDDPVLAARIDKMSREGRNAFLEYQLPEAVITLKQGAGRLIRD
                     EGDRGVLVICDGRLVSR"
BASE COUNT          576 a         1018 c         1017 g          586 t
ORIGIN      
        1 cgcgaatatc gagcaggcaa cgaaccttcc aaggttgact cgcatagcgt ctcctggctg
       61 agggatgacc catggtaaca agaacgattc tcaatagcaa tagagaacca ttctcgttgg
      121 aatactgcct ggcgggccgg gtttgcccta gttcgaaatt ctccgcgcgc cgttgtagcg
      181 ccgtagccaa tagcgctcgt tcatgctgac cacctccacc cgaccaccgt tgctaggtgc
      241 gtgaacgaag cggctttcgc cgaggtagac gccgacgtga gagtacggct cgcgcaaggt
      301 atcgaaaaac acgagatcgc caggcgccag ttcccggcgg cgcacgggcg cgccgacccg
      361 gctcatatcc tgtgcccggc gcggcagcgg cattcccgtg acgttggcaa ccacgtagtg
      421 aaccagcccg ctgcaatcga atcccgtatc gggagatcga ccgccatagc gatagggcac
      481 gcctacgaag tcgagcgcac gcagcgcgat ccggctaccg gggctaccgt cctgcgcact
      541 cctgcccggc cagtccgcag ccgggcgatg ctctgctgtg cgcgtggcgc atgcagtcaa
      601 acccgcaaga aaggcgagca cgagaattaa tctcatgcat caaatgtagc ccataaccct
      661 ctgatgtgca aagaccggtg gctacgtgct cccgctcgtt ggggcgggta ttcagctcgt
      721 gcggtagggc atggctcacc caggcgtcaa agccgcgggt gatatgagca caccgaattg
      781 ttcgcaccag atgacgacgc tgccatagcg agagacgtcc accccgtggg ggacggagta
      841 attctggctg cccttgaatg ccttcagcct tcccaggtcg atgaacatcg tgcgcgcgac
      901 gtcagtggac ggcgtcacgt tcctctcggg cacgagatag acgtgatact tcggccccgg
      961 tccgacttcg aaatccgctt cgagatacac gagatcttcg aaagccttga cctgcccttt
     1021 tccatagtga atcgggtcgg agggattcgc atgacggaag gtaccggttg ccacgagccg
     1081 cttcgatgtg cccgcatcca ggcgctcggt cgcaacgata tcggccagga aaatgtaggg
     1141 atagacgaag atcccgagcg cgaagcccgc cgcggcgccg agaagaccgc caattacgaa
     1201 gatgaggatg gatctcgcca caacgacgct ctttctgttt gtcagctggc ccgtccttag
     1261 gcgtcacgcg ctcgtaatcc ttacatggaa tggcgcgttg tgggtaacga tgcaacccgg
     1321 cagcgtggtc accggctgtg gcgcaggcta aactcgcggg cgtgtctcaa ttcgagtggt
     1381 tgttctccgc cgagggtcct cttgcgcggg caattggtga cttccgtgcg cggccgtttc
     1441 agctcgagat ggcgcgcgcg gtcgcggacg ccatagcgga aagccgaata ctggtggtcg
     1501 aggccgggac cggaacgggc aagactttcg cctatcttgt cccagcgctc atgtccggcg
     1561 ggaaggtgat cctctcgacg ggtacgaaaa cgctgcagga tcagctcttc gaccgcgaca
     1621 tccccaccgt ccgcagggcg ctcggcatcc cggtgactgt cgcgctgctc aagggacgtg
     1681 ccaattacgt ctgccactat catctggagc gtgcgcttgc cgaaggccgg tttcagagcc
     1741 gccaagagcc gcaggatctc gcgcgcatcg cggattttgc gcgctcgagc gtaacggggg
     1801 acaaagccga ttgtgcggtc gtgccggaaa acgccggtgc ctggtcgcat gccacgtcca
     1861 cccgtgacaa ttgcctcggc tccgagtgcg gacactacga gcgttgcttc gtgatggctg
     1921 cgcgacgcca ggcgctcgcc gccgatgtcg tagtcgtcaa ccatcacctg tttttcgccg
     1981 atctggttct gcgcgacgaa ggagcgggcg agctgctccc cgcgtgcaac acggtgatct
     2041 tcgacgaggc gcaccagttg ccggagaccg cgagcctctt ttttggcgaa agcgtttcca
     2101 cgacgcagta cgctgaactc gcccgggacg cgcgtgcgga aggggcggtg gcggcgcgcg
     2161 aatgccccga actgcagcgg gcggcgcacg cgctcgagaa ggccgcgcgc gatctgcgcc
     2221 tcgccttcac cgaggacaat ggtcgcctgc cgctccgtgc agtcgagcgc aacagcggct
     2281 ttgctccggt gctcgccgca gtgctcgcac gcctcgacga gctcgcacac atcctcgccg
     2341 agcaggccca gcgcagcgat ggcctggagc gctgctggca gcgcgcgctg tcgctggcgg
     2401 agcaaacgga gcgctggcgt agcgcggcag acggagcacg cgtgcgctgg gtcgagctct
     2461 tcagccgcgc gctccatctg cattccacgc ctctttcgat cgccgaccgg ttccatgacc
     2521 agatctcagc ccagccgcgc gcctggattt tcacttccgc gaccctttcg gtcgggggaa
     2581 acttcgacca ttatcgtagc gagctcggct tggaggatgg ggaggacaga agctgggaaa
     2641 gcccgttcga ttacgaacgt caggcgctcc tttacgtgcc taaaggattg cccgagccca
     2701 acacgccgga acacacggcg gcggtcgttt ccgccgcgct tccgcttatc gaggcgtgcg
     2761 gtgggcgcac gttcgtgctc tgcacgagcc tacgcgcgat gcgggaggcg tacctccttt
     2821 tgcatgacgc gtttcgccgg cgcagcctcg gctatccgct gctcgcgcag ggtgaggggt
     2881 cgcgcacgga gctcctggag cgcttccgca ccctcggtaa cgcggtgctc gtgggcagcc
     2941 actcgttctg ggaaggcgtc gatgtgcgcg gtaatgcact gtccctcgtc gtcatcgaca
     3001 agcttccctt ctcggcgccc gacgatccgg tgctcgccgc acgcatcgac aagatgagcc
     3061 gcgaaggccg caacgctttt ctcgagtatc agctgcccga ggccgtcatc acgctcaagc
     3121 agggggcggg gcgattgatc cgcgacgagg gcgaccgcgg cgttctcgtc atctgcgacg
     3181 ggcgtcttgt aagccgg
//
LOCUS       DASURG010000399         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1500942, whole genome shotgun sequence.
ACCESSION   DASURG010000399 DASURG010000000
VERSION     DASURG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500942"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFY80_07050"
     CDS             <1..958
                     /locus_tag="VFY80_07050"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6007702.1"
                     /translation="NLSLHPYEFREAGHSTHAFGPMFGSAAAAGSLAGLDARQARHLL
                     SYTAQQTSGVSCWMRDEEHIEKAFDFGGMPARNGVAAAAMVAHGFTAVEDVFSGERNF
                     FVAYDERHRIGRAPEPERLTRELGSTFEIMNTNIKRWSVGSPIQAPLDALLELIRTHN
                     IKADDVEKLVVRVAHQGANTTDNRHMPDICMQHMCAVMLIDGIVTFASAHDEKRMRDR
                     KVLALRSRIELYGDDELTRALPSRQGIVELKLKDGRELRNHVKAVRGTSHNPMNRAEV
                     DEKCFHLMAPILGKARARKLCDAVWALESTTNMQRLRSLLTA"
     gene            974..>2067
                     /locus_tag="VFY80_07055"
     CDS             974..>2067
                     /locus_tag="VFY80_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6007703.1"
                     /translation="MSEATARARLAQALFAPRTIALIGASGDATKNTSRPQRFLRKHG
                     YTGTIVPVNPGRTEIFGEKAYPDVTAVPHAIDHAFIMVPAKAVAAAVEQCVAKKVTVA
                     TIYSDGFAETGPEGRREQERLLAIAREGGVRLVGPNCIGLVATEPPCMLSVNAVLEMA
                     EIRKGPLAIVSQSGSMIGGLLSRGLGRGAGFSKLISVGNEADLGVGEITDLLVDDPHT
                     STILLFMETIRDAEHLARAARRAYAAGKAVIVYKLGRSEVGQDLAASHTGAMAGGDEV
                     ADAFFRAHGMLRVDVLEGLFELPALVQGQRPASRHRVAVMTTTGGGAASVVDRLGSFG
                     LEVVAPTGRVVANLAQKGIRIGTARLTDLTL"
BASE COUNT          374 a          683 c          698 g          312 t
ORIGIN      
        1 caacttgtcc ctgcatccgt acgaattccg cgaggcggga cattcgacgc acgctttcgg
       61 gccgatgttc ggctcggccg cggcagcggg cagtctcgcc ggcctggacg cgcgccaggc
      121 gcgccacctg ctctcgtaca ccgcgcagca aacctccggc gtgtcgtgct ggatgcgcga
      181 cgaggagcac atcgagaagg cgttcgactt cggcggcatg ccggcgcgta acggcgttgc
      241 agccgcagcg atggtcgctc acggcttcac cgcggtggaa gacgtcttct ccggcgagcg
      301 caacttcttc gtcgcctacg acgagcgcca tcgcatcggt cgcgcgccgg agcccgaacg
      361 cctgacgcgc gagctgggat ccacgttcga aatcatgaac accaacatca agcgctggtc
      421 ggtcggttcg ccaatccagg cgccgctcga cgcgctgctc gagcttatcc gcacgcacaa
      481 catcaaggcg gacgatgtgg agaaactcgt ggtgcgggtc gcgcaccagg gcgccaacac
      541 gaccgacaac cgccacatgc ccgacatctg catgcagcac atgtgcgcag tcatgctgat
      601 cgacggcatc gtcaccttcg cctcggcgca cgacgagaag cgcatgaggg accgcaaggt
      661 gctcgcgctc cgaagccgca tcgagctcta cggcgacgac gagctgacac gcgcgctgcc
      721 gtcgcgccag ggcatcgtgg agctcaagct caaggacggg cgggagctgc gcaaccatgt
      781 gaaagcggtg cgcggcacga gccacaatcc gatgaaccgt gccgaggtgg acgagaagtg
      841 cttccacttg atggcgccga tattgggcaa ggcgcgcgcc cggaagctgt gcgacgcggt
      901 gtgggcgctc gagagcacga cgaatatgca gcgcctgcgg tcgcttctca cggcatgagg
      961 cgcgtgtcgg gcgatgagtg aggcaacagc gcgcgcgcgg ctcgcacaag cgctgttcgc
     1021 gccccgaacg atcgcgctca tcggcgcctc gggcgacgcg accaagaaca cctcgcgccc
     1081 ccagcgattc ctgagaaagc acggctacac cggcacgatc gttccggtga atccgggacg
     1141 caccgaaata ttcggtgaga aggcctaccc cgacgtcact gctgtcccgc atgcgatcga
     1201 ccacgccttc atcatggtgc cggcgaaagc ggtggccgcc gcggtggaac aatgcgtggc
     1261 gaagaaagtg accgtcgcca cgatctacag cgacggcttc gcggagaccg ggcccgaagg
     1321 gcgccgcgag caggagcgcc tgcttgcgat tgcgcgcgaa ggcggcgtcc gcctcgtcgg
     1381 cccgaactgc atcggactcg tcgcgaccga gccgccctgc atgctgtcgg tcaacgctgt
     1441 cctcgaaatg gcggaaatcc gcaagggacc gcttgcgatc gtgtcgcaaa gcggcagcat
     1501 gatcggaggg ctgctgtcgc gcgggctggg acgcggcgcc ggcttctcca agctgatctc
     1561 ggtcggcaac gaagccgatc tcggcgtcgg cgaaatcacc gatctcctcg tcgacgatcc
     1621 gcacacgagc accatcctcc ttttcatgga gacgatccgc gatgcggagc atctcgcgcg
     1681 ggcggcccgc cgggcatacg ccgccggaaa agcggtaatc gtgtacaagc tcgggcgctc
     1741 ggaagtgggc caggacctcg ctgcatcgca caccggcgca atggcaggcg gggacgaagt
     1801 ggcagatgcc tttttccgtg cgcacggcat gcttcgagtg gacgtgctgg aagggctctt
     1861 cgagctgccc gcgctggtgc aagggcagag accggcgtcg cgtcaccgcg tcgccgtgat
     1921 gacgaccacc ggcggcggcg cggcgagcgt ggtggatcgc ctcggcagct tcgggttgga
     1981 ggtggtcgcc cccaccggtc gggtcgtggc gaatcttgcg cagaagggta tccgcatcgg
     2041 cacggcacgg ctcaccgatc tcacgct
//
LOCUS       DASURG010000400         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1501452, whole genome shotgun sequence.
ACCESSION   DASURG010000400 DASURG010000000
VERSION     DASURG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501452"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFY80_07060"
     CDS             <1..999
                     /locus_tag="VFY80_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6007704.1"
                     /translation="ETAAFLLAGDDLAARFAMTRLFEPDQFLARQNVVHLSAVAPGES
                     ALSGTLVLSREHLHRFTTGLERKPGFSAEFPARLIRTDLAWEQLVLPRSTLEQLEEIQ
                     RWILHGGALLRDWGMGGRLRPNFVSLFHGAPGTGKTLAAGLVGRHCGCEVYKLDLSMI
                     VSKYIGETEKNLAKVFDLAEHRRWILFFDEADALFGRRTRVESSHDRYANQEISFLLQ
                     RIEEFNGVVILASNLKSNVDDAFLRRFQSVVHFPMPRPAERKRIWQNAFSPRAVLEDG
                     IDLGRLAEKHELSGGTIMNVVRYSSLKALSRNQSVIWLADMEEGIRREFLKEGRTS"
     gene            1015..>2437
                     /locus_tag="VFY80_07065"
     CDS             1015..>2437
                     /locus_tag="VFY80_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX6007705.1"
                     /translation="METARAKVVNRPPTSAPAAARGIQRKALRVSSSSDPAEREAEST
                     ARKVARMPLADTSVRHRAGIFRSPLVQNTPLIQRKAEGQPDTGANIAAEIQSSSTEGT
                     PLPLSVRRFMEPRFRADFSKVRMHNNEKSAALSTHVGARAFAVGNQIFFGRDQFRPET
                     PDGRELIAHELTHTIQQGGAIQRSEDVSVTQQTPTMVQRLGISDALDYFADKAHNIPG
                     FRMFTIILGVNPINMSRVERTPANILRGVIEFVPGGALVTQALDNYGIVERVASWAAE
                     QIRSLGMTGSIIRDAIMRFLDSLGWRDIFRLGSVWDRAKRIFTEPIDRIISFARNLIT
                     GILRFIREALLRPLARLAEGRPAYDLLKAVLGQDPITGEPYPRNADTLIGGFMKLIGQ
                     EEIWQNIKRANAVARAWAWFQGALSGLLGFVRQIPTLFVQALQSLTLADVVLLPRAFA
                     KVAGVFAGFAIRFISWAGSQVLSL"
BASE COUNT          428 a          813 c          776 g          420 t
ORIGIN      
        1 gagaccgcgg cgttcctgct cgccggcgac gacctcgcgg cgcgcttcgc catgacgcgc
       61 ctgttcgagc ccgaccagtt cctcgcgcgg cagaacgtgg tgcacctctc ggcggtggcg
      121 cccggcgagt cggcgctgag cggcacgctg gtgctctcgc gcgagcacct gcaccgcttc
      181 accaccggcc tggagcgcaa gcccggcttc agcgccgagt tcccggcgcg cctcatccgc
      241 accgaccttg cctgggagca gctggtcctg ccgcgctcga cgctcgagca gctcgaggag
      301 atccagcgct ggatcctgca cggcggcgcg ctgctgcgcg actggggcat gggcggccgg
      361 ctgcggccga atttcgtgag cctgttccac ggcgcgccgg gcaccggcaa gacgctcgcg
      421 gcgggcctgg tcggccggca ttgcggctgc gaggtctaca agctcgatct cagcatgatc
      481 gtgtcgaagt acatcggcga gaccgagaag aacctcgcca aggtattcga cctcgccgag
      541 caccgtcgct ggatcctgtt cttcgatgaa gccgatgcgc tattcggccg gcgcacgcgt
      601 gtcgaaagct cgcacgaccg ctacgccaac caggaaatca gcttcctgct ccagcgcatc
      661 gaggaattca acggtgtggt catcctggcg tccaacctca agagcaatgt cgatgatgcc
      721 tttctgcgcc ggttccagtc ggtcgtgcat ttccccatgc ccaggccggc ggagcgcaag
      781 cgcatctggc agaatgcctt ctcaccgcgc gccgtgctgg aagatggcat cgacctgggg
      841 cgcctggcgg aaaagcacga gctgtcgggc ggcaccatca tgaacgtggt gcgctactcc
      901 tcgctcaagg cactgagccg caatcagtcg gtcatctggc tggctgatat ggaagaaggc
      961 attcgtcgcg aatttctgaa ggaaggccgc acgtcgtgaa cgcgagccaa tagcgtggag
     1021 accgcacgcg ccaaggtcgt caatcgcccg ccgacgtcgg cacccgcggc agcgcgtgga
     1081 attcagcgca aggcgctgcg cgtatcgtct tcgagcgatc cggctgaacg ggaagcggag
     1141 tcgacggcca gaaaagtggc acgcatgccg ctggcggaca cttccgtccg ccatcgtgcc
     1201 ggcatcttcc ggtcgcctct cgtgcagaac accccgctga ttcaacgcaa ggcggaagga
     1261 cagccggaca ccggcgccaa catcgccgcc gaaatacaga gcagttccac cgagggtacg
     1321 cccctgcccc tctctgtgcg ccgtttcatg gagccgcgtt tccgcgccga cttcagcaaa
     1381 gtgcgcatgc acaataacga gaagtccgcc gcactcagca cgcatgtcgg cgcgcgcgct
     1441 ttcgccgtag gcaatcagat ctttttcggt cgtgatcagt tccgcccgga aacgcccgat
     1501 ggacgcgaac tgattgccca cgagctgacg cacaccatac agcagggtgg cgcgatccag
     1561 cgcagtgaag atgtgagtgt cacgcagcag acgcccacca tggtgcagcg gctgggcatc
     1621 agcgatgcgc tcgattattt cgccgacaag gcgcacaaca tccccggctt ccgcatgttc
     1681 accatcatcc tgggggtgaa tcccatcaac atgtcgcggg tggagcgcac gcctgccaac
     1741 atcctgcgcg gcgtcatcga gtttgtgccg ggcggcgcgc tggtcacgca ggcgctcgac
     1801 aattacggca tcgtcgagcg cgtggcgtcg tgggcggcgg agcagatccg gtcgctgggc
     1861 atgaccggca gcatcattcg cgatgcgatc atgcgtttcc tcgattcgct cggctggcgc
     1921 gacatcttcc ggctgggcag cgtgtgggat cgcgccaagc gcattttcac cgagccgatc
     1981 gaccgcatca tcagctttgc ccgcaatctg atcaccggca tcctcaggtt catccgggaa
     2041 gcgctgctca gacctctcgc gcgcctggcg gaaggcagac cggcctacga cctgttgaaa
     2101 gccgtgcttg ggcaggaccc gatcaccggc gagccgtatc cgcgcaacgc cgatacgctc
     2161 atcggcggat tcatgaagct catcggccag gaggagatct ggcagaacat caagcgcgcg
     2221 aatgcggtgg cgcgcgcgtg ggcgtggttc cagggcgcgc tcagcggact gctcgggttc
     2281 gtgcggcaga ttcccacact gttcgtgcaa gcgctgcaat cgctcaccct ggccgacgtc
     2341 gtgctcttgc cacgcgcgtt cgccaaagtg gccggcgtat tcgccggctt cgccatccga
     2401 ttcatcagct gggcaggcag ccaggtgctg agcctgc
//
LOCUS       DASURG010000401         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_70586, whole genome shotgun sequence.
ACCESSION   DASURG010000401 DASURG010000000
VERSION     DASURG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70586"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFY80_07070"
     CDS             complement(<1..325)
                     /locus_tag="VFY80_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007706.1"
                     /translation="MKFEIPQSLKSEHEELHSTLVRATKESGLTGVAAKAVAALLHPH
                     FVKEEEFALPPLALLAPLSRGEWAAEMRDVLALTEKLKAELPQMLSEHRSIVVALDKL
                     EEAARS"
     gene            complement(380..1006)
                     /locus_tag="VFY80_07075"
     CDS             complement(380..1006)
                     /locus_tag="VFY80_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6007707.1"
                     /translation="MNSRKLFGRLAAAAGFSLGLLIAPGTALAHCDTLDGPVVAAARK
                     ALDTNNVNLVLPWVQKKDEADIRSQFQKTVAVRKAGGQAKELADTYFFETLVRIHRAG
                     EGAGYTGLKPAGQIEPPIAAADKSLDSGKLQDVAQLISKRTEQGLHRHFEDVMKKKNY
                     KPNDVAAARAYVSAYVEYTHYVERLYNAAETLAPEHVQKTAPGLAHTH"
     gene            complement(1099..1566)
                     /locus_tag="VFY80_07080"
     CDS             complement(1099..1566)
                     /locus_tag="VFY80_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX6007708.1"
                     /translation="MNRTIVALIVSVMVNVGVGAALAYSAFQHGTWPAVFRGDKGEPD
                     LPAYLDLNAEQKRRWSELEAAFLRELGKDWRQIRIHRERMINEIFSEQPDRQRIEEER
                     AAIAQLQSAQQRRVIQQLLQERDILDAAQRRKLAKMLLRQAPAGTFEERLHGK"
     gene            complement(1568..2038)
                     /locus_tag="VFY80_07085"
     CDS             complement(1568..2038)
                     /locus_tag="VFY80_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6007709.1"
                     /translation="MKERPEPQCPRTEALSALIDEELAGGARSEMVTHAASCPLCGAM
                     LADLQRLRVALKPLAAARPGVDLAPLIEQRLRDKGVPSDSSHRPSRVARPRSPWTLFP
                     VAITAAGALALGVYFGALLAGGGTASMLRPASMALFDPIPPGGLCVGLPACDGR"
     gene            complement(2035..>2287)
                     /locus_tag="VFY80_07090"
     CDS             complement(2035..>2287)
                     /locus_tag="VFY80_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6007710.1"
                     /translation="DYDAPDGGPAPEAQLQAKQQLKALDTALRRLPSDQREIVLLREI
                     EDMSYSEISATLGISEGTVKSRLARAREALLSHYREADA"
BASE COUNT          389 a          756 c          705 g          437 t
ORIGIN      
        1 cagatcgtgc cgcctcttcg agtttgtcga gggcgaccac gatggatcga tgctcgctca
       61 gcatttgcgg cagctccgcc ttgagctttt cggtaagggc cagcacatcg cgcatctcgg
      121 ccgcccactc tccacgcgag agaggcgcaa gaagagccag cggaggcaac gcgaactctt
      181 cttccttgac gaaatgagga tgcaagagcg ccgccactgc cttcgctgcc actccggtca
      241 gcccgctttc ctttgttgcc cggaccagcg tcgaatgcaa ctcctcgtgt tcgctcttca
      301 gggattgggg aatctcgaat ttcatgaata gtctcccggt gcggggcagc gccgaagcgc
      361 ggccccgatg gcgcgatgac taatgcgtat gcgcgagccc cggcgcggtt ttctgcacgt
      421 gctccggtgc cagggtctcg gccgcgttgt agagccgctc gacatagtgc gtgtactcga
      481 catacgcgct cacgtaggca cgcgcggcgg cgacgtcatt cggtttgtag ttctttttct
      541 tcatgacgtc ttcgaagtgc cggtgcaatc cctgctcggt ccgcttggaa atgagctgcg
      601 ccacgtcctg aagcttgccg ctgtcgagcg acttgtcggc agccgcgatc ggcggctcga
      661 tctggccggc gggcttgaga cccgtgtatc cggcgccctc acctgcgcga tgaatgcgca
      721 cgagggtctc gaagaaatac gtgtcggcaa gctccttcgc ctgcccgccg gctttacgca
      781 ctgccacggt cttctggaac tggctgcgga tatcggcttc gtccttcttc tggacccagg
      841 gcagcaccag attgacgttg ttggtgtcga gcgccttgcg agccgccgcg acgacgggcc
      901 cgtccagggt gtcgcaatgc gcgagcgccg tgcccggtgc gatgagcagt cctaaagaaa
      961 accccgccgc ggccgccagg cggccgaaaa gtttacgaga gttcatgatg gtcaagctcc
     1021 tgtcggttga gaggcgcgaa aatcgcagcc tttgttccgt taaacgagcc ggcggggaat
     1081 atgttccatc atgatgtgct atttgccgtg cagccgttcc tcgaacgtgc ccgccggcgc
     1141 ctggcgcaga agcatcttcg cgagcttgcg ccgttgcgcg gcatccagga tgtcgcgctc
     1201 ctgcaggagc tgctggatca cgcgtcgctg ctgtgcgctc tgcaactggg ctatcgcggc
     1261 ccgttcctct tcgatgcgct gccgatccgg ctgctcggaa aagatctcgt tgatcatgcg
     1321 ctcacggtgg attcgaatct gccgccaatc ctttcccaac tcgcgaagaa aggcggcttc
     1381 gagttcgctc cagcggcgct tctgctcggc gttgagatcc agatatgccg gcagatccgg
     1441 ctcgcctttg tcgccgcgaa aaaccgcggg ccacgtcccg tgctgaaatg ccgaatacgc
     1501 tagcgcagca cctacaccca cgttgaccat gacggaaacg atgagcgcga cgatcgtgcg
     1561 attcacgtta cctcccatcg cacgctggca gcccgacgca gagcccaccc ggcgggattg
     1621 gatcgaagag cgccatggaa gcgggtcgaa gcatgctcgc cgtgccgccc ccggcgagca
     1681 gtgccccgaa atagacaccg agcgccagcg cgcctgccgc cgtaatcgcc accggaaaga
     1741 gtgtccacgg ggatcgcggg cgcgcgacgc gtgagggtcg gtgagacgag tccgaaggga
     1801 cccccttgtc ccgcagccgt tgctcgatca gcggcgcgag atcgacgccg ggtcgcgcag
     1861 ccgcaagcgg ctttagcgcc acccgaaggc gctggagatc cgcgagcatc gcgccgcaca
     1921 gcggacacga cgccgcgtgc gttaccattt cgctacgagc cccgccggca agctcctcgt
     1981 cgatcaacgc cgaaagcgcc tcggtgcgcg gacactgcgg ttccggtctc tccttcatgc
     2041 atccgcctca cggtaatgcg aaagcaacgc ctcgcgggca cgtgcaaggc gggatttcac
     2101 ggtgccctcg ctgataccca gcgtcgcgct gatctcgctg tacgacatgt cttcgatctc
     2161 gcgtaacagt acgatctcac gctgatcgga cggaagacgc cgcaacgccg tgtccagcgc
     2221 cttcagctgc tgcttcgcct gcaactgcgc ttccggcgcc ggcccgccgt ccggcgcgtc
     2281 gtagtcc
//
LOCUS       DASURG010000402         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_683776, whole genome shotgun sequence.
ACCESSION   DASURG010000402 DASURG010000000
VERSION     DASURG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683776"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2223)
                     /locus_tag="VFY80_07095"
     CDS             complement(<1..>2223)
                     /locus_tag="VFY80_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEX6007711.1"
                     /translation="GIGFLVVLYARYYMSPQDPVPRFFAFLLAFMGSMLGVVLSGNLL
                     LMVFFWELTSVFSFLLIGYWHHNAAARDGARMALIVTSAGGLCLFAGVLIIGRIVGSY
                     DLDEVLAGRETIQAHALYVPALVLIAAGALTKSAQFPFHFWLPHAMAAPTPVSAYLHS
                     AAMVKLGVFLLARMWPVLGGTDAWIWIVGTAGFATLLFGAYMAIFQHDLKGLLAYSTI
                     SHLGLITALLGLNRPLALVAAVFHIMNHATFKASLFMAAGIIDHETGTRDLRRLNGLF
                     RFMPITATLAMVAAAAMAGVPLLNGFLSKEMFFAESLHAEGPLPVLNQALPVIATAWG
                     IFSVAYSLRFIHGVFFGPDPTGLPREPREPPRWMRFPVELLVLACLVVGILPVLTVGP
                     VLDIAVYSVLGPFKPPYSLAVWHGFTTAVFMSIAAMGGGIVLYVMLHHYLSQGAERTP
                     LLPPLDGRRLFERLLVLLSLHWARALETALGTRRLQVQLRWVLVAALFAALWRLSLNG
                     LEWGPLPLTPIDPVFLLMWIAGGACAFGAAWQAKFHRLVALVLMSGAGLVVCLTFVWL
                     SAPDLALTQLLVEIVTAVLLLLGLRWLPKRIPFRWTLAGALAAFPRRLRDFVIATAAG
                     SGLAVVAYAVMTRPLPDTISHFFIEEAWREGGGTNVVNVIIVDFRGFDTLGEITVLGV
                     VAVAVYSLLRRFRPARESVEPLAQHTRQSEAVAAEDLLVPAVIMRLMFPPIALFAVYL
                     L"
BASE COUNT          507 a          707 c          732 g          277 t
ORIGIN      
        1 agcaggtaga cggcgaaaag agcgatcggc gggaacatga ggcgcatgat cactgcggga
       61 accaacaggt cttcagcggc caccgcctcg ctctgccgcg tgtgctgcgc cagcggttcg
      121 acgctctcgc gcgcgggacg aaaccgacgc agcagcgagt acacggccac cgccacgacg
      181 ccgagtaccg tgatctcgcc caatgtgtcg aagccgcgga agtcgacaat gatgacgttc
      241 acgacgttcg ttccgccacc ctcgcgccac gcctcttcga tgaagaaatg cgagatcgta
      301 tcgggcagcg gccgcgtcat cacggcatag gcaaccacgg cgagccccga tcccgccgca
      361 gtggcgatca cgaagtcgcg caggcgccgc ggaaaagccg caagcgcgcc cgcgagcgtc
      421 catctgaagg gaatgcgctt ggggagccag cgcaagccga gcagcagcag tacggcggtt
      481 acgatttcca ccaggagttg cgtaagagcg agatcgggcg cggagagcca cacgaaggtg
      541 aggcagacca cgagcccggc gccgctcatc agcacaagag cgaccaggcg gtggaatttc
      601 gcctgccacg cggcaccgaa tgcgcaggcg ccgccggcga tccacatgag gagaaatacc
      661 ggatcgatgg gcgtcaaagg caacggtccc cattccagcc cgttcaggga aagccgccac
      721 aacgccgcaa aaagcgctgc gacgagcacc cagcgaagct gcacttgcag ccgccgtgtg
      781 ccgagcgccg tttcaagcgc gcgcgcccaa tgcagcgaca gcaggaccag caggcgctcg
      841 aagagccgtc gcccatcgag aggcggcaaa agcggcgtgc gctccgcgcc ctgcgagagg
      901 taatggtgca gcatcacgta aagcacgatg cctccaccca tcgcggcgat actcatgaac
      961 accgcggtcg tgaacccgtg ccacacggcg agactgtagg gcggcttgaa cgggccgagc
     1021 acggagtaca ccgcgatgtc cagtaccggg cccacggtga ggacgggcag gatcccgacg
     1081 acgaggcaag cgagcaccag caactccacc ggaaagcgca tccagcgcgg cggctcccgc
     1141 ggctcccgcg gcaggcctgt cggatcgggt ccgaagaaga cgccatgaat gaatcggagc
     1201 gagtatgcga cgctgaaaat accccacgcg gtggcgatga ccggcaaggc ctggttgagc
     1261 actggcagcg gcccctccgc atgaagcgac tcggcgaaga acatctcctt ggaaagaaag
     1321 ccgttcagca gtggcacgcc cgccatcgcc gccgccgcaa ccatcgcgag cgtggcggta
     1381 atcggcatga aacggaaaag accattcagc cggcgaaggt cgcgcgtgcc ggtctcgtgg
     1441 tcgatgatgc cggccgccat gaaaagcgaa gccttgaaag tcgcgtggtt catgatatgg
     1501 aaaaccgcgg ctacgagcgc aagcgggcgg ttcagtccga gcagcgccgt gatgagaccg
     1561 agatggctga tcgtcgagta agcgagcagt cccttgaggt cgtgctggaa gatcgccatg
     1621 taggccccga acaacagtgt ggcaaaaccg gcggtgccga cgatccagat ccatgcgtcg
     1681 gtaccgccca atacgggcca catgcgggcg agcaggaaca cgccgagctt gaccatcgcc
     1741 gcggaatgaa gataggccga caccggcgtc ggtgccgcca tcgcgtgcgg cagccagaag
     1801 tgaaacggga actgcgcact cttggtgagc gcgcccgctg cgatcagcac gagcgccggc
     1861 acgtagagcg catgcgcctg gatcgtctcg cgccccgcca gcacttcgtc caggtcgtag
     1921 ctgcctacga tgcggccgat gatgagcacg cccgcaaaca ggcacaggcc gccggcggag
     1981 gtgacgatga gcgccatgcg cgcgccgtcg cgtgccgccg cgttgtgatg ccagtatccg
     2041 atcagcagaa acgagaagac gctcgtaagt tcccagaaga acaccatgag cagcagattg
     2101 ccggagagaa ccacgccgag catcgacccc atgaacgcga ggaggaacgc gaaaaagcgc
     2161 ggcaccgggt cctgcgggga catgtagtaa cgcgcgtaga ggacaacgag aaatccgatg
     2221 ccg
//
LOCUS       DASURG010000403         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_990350, whole genome shotgun sequence.
ACCESSION   DASURG010000403 DASURG010000000
VERSION     DASURG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990350"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFY80_07100"
     CDS             complement(<1..198)
                     /locus_tag="VFY80_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6007712.1"
                     /translation="MKIGFIGLGQMGRGMAARLVERGHALNVWNRTQAAAEAFRTRGV
                     TVADRADQVLAADVVITMLADD"
     gene            336..1130
                     /locus_tag="VFY80_07105"
     CDS             336..1130
                     /locus_tag="VFY80_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6007713.1"
                     /translation="MDLGIKGRSAIVTGASRGIGKETARQFLEEGVRVTICGRNAETL
                     ERTREELVKGTGGEIHAVVADMMKADDIARLVESARKQFDGIDILVNNAGQMYSGRFD
                     VMTDTGLREQLETKLFGFLRAIRLVHPLMKAQRWGRIVNLIGGAGKEPDPYMFGSGIT
                     NSALLNITKSLSTEFGEDNILVNAVCPGWVDTNLWRRNARGLASELGAKSEEEARRLA
                     ARKNSLNRFGRPEELANAIVFLCSERASYITGVSLNLDGGRLKSLW"
     gene            1245..2213
                     /locus_tag="VFY80_07110"
     CDS             1245..2213
                     /locus_tag="VFY80_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007714.1"
                     /translation="MKCVRVALLLSVIWPAAVLPVHAQQYPTRPVRLIIPWPPGGVTD
                     VIARGLGATLTESLGQQVIPDNRPGAAGTLGVGIAASANPDGYTLLMTDVPSHAISAT
                     LRPNLPYNPLKDIEPIALPSRSPLVLVVNPKFGIKTIPQLIAHAKAKGSEFSFASSGP
                     GSITHLTAERFIRETGVKPLHVPYKGGGPATAALVSGEAGMYFACISAAIPHIKAGRL
                     TLLGITSPKRSTLFPDTPAVAEVLPKFEMGCNTGFFAPGGTPKPIIERLHAAAMKAVQ
                     HPRMKDLLAANAAEAAPYTQAQFKQHVADEVRSWGAVVKAAGLKVE"
     gene            2224..>2498
                     /locus_tag="VFY80_07115"
     CDS             2224..>2498
                     /locus_tag="VFY80_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007715.1"
                     /translation="MRELNGKVALITGASRGIGQAIALRLAAEGAALYLAADGTQVEL
                     EDTASACRERGAPDAAWDLHDLAADGAAEAMISTAHKRMGRIDILVN"
BASE COUNT          476 a          832 c          795 g          395 t
ORIGIN      
        1 atcgtccgcg agcatggtga tgacaacatc ggcggcgagc acttgatccg cacggtccgc
       61 cacggtgact ccccgcgtgc ggaacgcctc ggcagccgcc tgcgtgcggt tccatacgtt
      121 gagggcatgg ccccgctcga caaggcgcgc ggccatgccg cggcccatct ggcccagacc
      181 tatgaatccg atcttcatat cgctgctcct tccattcgtg cacaacccgc aggtcgcgcc
      241 accgcgccgt tgaattacaa tgcgacagga gtccttcaag gagccgccat tatgccggct
      301 ccctttctat cgcgaccatc tgcaaggaaa acaacatgga cctaggcatc aagggacgct
      361 ccgccatcgt aacgggcgcg agccgcggca tcggcaagga gacggcgcgg cagttcctcg
      421 aagaaggcgt gcgcgtgaca atctgcgggc gcaatgcgga gacgctggag cgcacccgcg
      481 aagagctggt gaaagggacc ggcggcgaaa tacatgccgt cgtcgcggac atgatgaagg
      541 ccgacgacat cgcgcggctg gtggagagcg cgcgcaaaca gttcgacggc atagatatcc
      601 tcgtcaataa cgccgggcag atgtactccg gccgcttcga cgtgatgacg gacaccggac
      661 tccgggagca gctcgagacc aagctgttcg gtttcctgcg cgcgatccgc ctcgtccacc
      721 cgttgatgaa ggcgcagcgc tgggggcgca tcgtcaatct gatcggcggg gcgggcaagg
      781 agcccgatcc gtacatgttc ggcagcggca tcaccaacag cgcgctgctc aacatcacca
      841 agtcgctctc caccgaattt ggcgaagaca atattctcgt gaacgcggtg tgccccggct
      901 gggtcgatac gaatctctgg cgccgtaatg cccggggact ggcatcggaa ctcggcgcaa
      961 agagcgaaga ggaggcgcga cggctcgcgg cgcgaaagaa ttcgcttaac cgcttcgggc
     1021 gaccggaaga gctggcgaac gcgatcgtct tcctgtgctc ggagcgcgcg agctacatca
     1081 ccggcgtctc gctcaatctc gacggcggac ggctcaaatc gctgtggtag cgacgccgtc
     1141 gttgacatgc cgagcgcgaa agttgtaaat agcgccggac aggggcgaca cacccgatcg
     1201 atccggaatc cgtttcctgg ttcacaaaag cgaggaggaa gatgatgaag tgcgtacgcg
     1261 ttgctttgct gctttcggta atctggcccg ccgctgtact gcccgtccac gcgcagcaat
     1321 atccgacgcg acccgttcgt ctcatcatcc cctggccgcc gggcggcgtt acggacgtga
     1381 tcgcgcgcgg cctcggcgcg acgctgaccg aatcgctcgg ccagcaggtg atccccgaca
     1441 accgtcccgg cgcggccggc acgctcggcg tggggatcgc ggcaagcgcc aatccggacg
     1501 gctacacgct gcttatgacc gacgtgccga gccacgccat cagcgcaacg ctgcgcccca
     1561 atctgccgta taacccgctg aaggatatcg agccgatcgc cctgccttcg cggtcgccgc
     1621 tcgtgctcgt cgtcaatccc aagttcggca tcaagacgat cccccagctc atcgcgcatg
     1681 ccaaggcgaa gggcagcgag ttttcgttcg cctcttcggg ccccggctcg atcacgcacc
     1741 ttacggcgga gcgcttcatc cgtgaaaccg gcgtcaagcc cctccatgta ccgtacaaag
     1801 gcggtggacc ggcgacggcg gcgctggtgt ccggcgaggc gggcatgtac ttcgcctgca
     1861 tatcggccgc gatcccgcac atcaaggccg gccggctgac gctcctcggc atcacgtctc
     1921 ccaaacgttc gacgctgttt ccggacacgc ccgcggtggc cgaagtgctg ccgaagttcg
     1981 agatgggctg caacaccggt ttctttgcgc cgggcggcac gccgaagccg atcatcgagc
     2041 ggctgcacgc ggcggcgatg aaagcggtgc agcatccccg catgaaggac ctgctggcgg
     2101 ccaatgcagc cgaagccgcg ccgtacacgc aggcgcaatt caaacagcac gtcgccgatg
     2161 aggtgcgcag ctggggcgcg gtcgtaaaag ccgccggact gaaagtcgag taatacagcg
     2221 ctcgtgcgcg agttgaacgg caaggtcgcg ctcatcaccg gtgcctcgcg cggcatcggg
     2281 caggctatcg cgctgcggct cgccgctgaa ggcgccgcgc tctacctcgc cgccgacggc
     2341 acgcaagtgg aactcgagga caccgcctcc gcatgccgcg agcgcggcgc gccggacgcc
     2401 gcgtgggacc tgcacgacct tgccgcggac ggggcagcgg aagcgatgat ctccaccgcc
     2461 cacaagcgca tgggccgcat cgacatcctg gtcaacaa
//
LOCUS       DASURG010000404         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1195079, whole genome shotgun sequence.
ACCESSION   DASURG010000404 DASURG010000000
VERSION     DASURG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195079"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFY80_07120"
     CDS             <1..378
                     /locus_tag="VFY80_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6007716.1"
                     /translation="DVSERTWQKEDRTLWRAKNCDTFKPMGPFIVTDLDPMNLTIAIH
                     VNGKKVSEYHTGKMLFSAQHYIAQISKYMTLWPGDVVWLGTDNATEPDLKHGDCVEVI
                     QKDIGILSNPVVRESRETANGER"
     gene            420..1586
                     /locus_tag="VFY80_07125"
     CDS             420..1586
                     /locus_tag="VFY80_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX6007717.1"
                     /translation="MAHFFASCPRGLEAVLTTELPTLGARDVNAVDGGATFSGEMALC
                     WAVNLESRVASRVLWQVGFARYGSEEDVYAAARRLPWNDWFDVQRSIRVNVSAIRSPL
                     KSLDFATLRIKDAVCDAFRAACGRRPDVDTAQPDIRIHAFLTRDTATFYLDTSGDALF
                     KRGWRVAGGEAPLRENLAAGILRLAGWQPGTALYDPMCGSGTFLVEAAMIALERAPGL
                     GRPFAFEKLASFEPATWRTLRERAKSRCRPACALSIHGSDKFGSVLDLARANLEAAGL
                     EDAVTLKQVDVLDSSPPAKSGIIVMNPPYGERLEDRDSLAAFYPLLGNVLKKRYAGWT
                     AYILTADMRLPGLMRLKPSRRTPLYNGALECRLFEFAMIAGSLRRGGADPRQPG"
     gene            1674..2087
                     /locus_tag="VFY80_07130"
     CDS             1674..2087
                     /locus_tag="VFY80_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007718.1"
                     /translation="MEKERTLKILNALANGVHPATGEQFPADSPYQHPDTVRALFEAV
                     RTVEHARAAAAERPQRPASMPGNTGLRWTSEEEERLIAAFDAGRTVDELARAHNRTRA
                     GIEARLVKLGKLDESAVTVQLRYPPKPSGQAQPGA"
     gene            complement(2117..>2368)
                     /locus_tag="VFY80_07135"
     CDS             complement(2117..>2368)
                     /locus_tag="VFY80_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007719.1"
                     /translation="TVLRATLSNIVLLSTSIRALVFLASGLMLADRVIGFALLLPFAL
                     TGLWIGNRYHGRISRGALLRLISVLLVLIGASLLLRATR"
BASE COUNT          456 a          763 c          774 g          375 t
ORIGIN      
        1 gacgtgagcg agcgcacctg gcagaaggag gatcgcacgc tgtggcgcgc aaagaactgc
       61 gataccttca agcccatggg accctttatc gtgacggatc tcgatccgat gaatctcacc
      121 attgcgatcc acgtgaacgg caagaaggtg tctgaatacc acaccggcaa aatgctgttt
      181 tccgcgcaac actacatcgc gcagatctcg aaatacatga cgctctggcc cggcgacgtc
      241 gtctggctcg gcaccgataa tgcgaccgag ccggacttga agcacggcga ctgcgtcgag
      301 gtcatccaga aggacatcgg cattctgtcc aatccggtcg tgcgcgaatc gcgtgaaaca
      361 gcaaacggtg aacggtgaat ggtgagcggt gaacaccgtt caccgttcac ttaatcctga
      421 tggctcattt tttcgcgtcg tgcccgcgcg ggctggaggc ggtgctcacc actgaactcc
      481 ccacgctcgg cgcgcgggac gtcaacgccg tggacggcgg cgcaactttt tccggtgaga
      541 tggcgctgtg ctgggcggtc aatctggaga gccgcgtggc gagccgggtg ctgtggcaag
      601 tgggattcgc acgctacggc tccgaggaag acgtttacgc tgcggcacgg cgtttgccgt
      661 ggaacgactg gttcgacgtg cagcgctcga tacgcgtcaa cgtctccgcg atacgctcgc
      721 cgctgaagag cctcgacttt gcgaccctgc gcatcaagga cgcagtatgc gacgcgttcc
      781 gcgccgcgtg cggccgccgg cccgacgtcg ataccgccca gcccgacatt cgcatacacg
      841 cctttctcac gcgcgatacc gcaacctttt atctcgacac gtcgggcgac gcgctcttca
      901 agcgtggctg gcgcgtcgcg ggcggcgaag cgccgctgcg ggaaaatctc gctgccggca
      961 tccttcgtct cgctgggtgg cagcccggaa cggcactcta cgacccgatg tgcggcagcg
     1021 gcacgtttct cgtcgaagcc gccatgatcg ctctcgaacg ggcgcccgga ttgggacggc
     1081 cgttcgcctt cgagaagctc gcgagcttcg agcccgcgac atggcgcaca ctgcgggagc
     1141 gtgcaaaatc acggtgccgg cccgcttgcg cgttgtcgat acacggcagc gacaagttcg
     1201 gcagcgtgct cgatctcgcg cgcgccaatc tggaggcggc agggctcgag gatgcggtca
     1261 cactgaaaca ggtcgacgtg ctcgactcga gtccaccggc gaagagcggg atcatcgtga
     1321 tgaatccgcc ctatggagaa cgcctcgagg atcgtgattc gcttgccgcc ttctatcccc
     1381 tgctcggaaa cgtactgaag aagcgctacg ccggctggac cgcctacatc cttaccgccg
     1441 acatgcgcct ccccgggctg atgcgtctca agccgtcccg gcgcacgccc ctctacaacg
     1501 gcgcgctcga gtgccgtctc ttcgaattcg cgatgatcgc cggcagcctg cgcagaggag
     1561 gcgctgaccc gcgtcaaccg gggtgacggg cgctgcgttg gaggtacgcg cattcgcgct
     1621 gtgagccggg cgcggcaacg cgccgatcac cccaaacgac aggaggaggc gtcatggaaa
     1681 aagaacggac tttgaaaata ctcaacgcgc tggcgaacgg cgtgcacccc gcaaccggtg
     1741 agcagtttcc cgcggacagt ccctatcagc atcccgatac ggtgcgggcg ctgttcgagg
     1801 cagtgcgcac ggtggagcac gcgcgcgctg cggcggcaga gcgcccgcaa agacccgcat
     1861 ccatgccggg caataccggg ttgcgctgga catccgagga ggaagagcgg ctgatcgctg
     1921 cattcgatgc cgggcgcacc gtcgacgagc ttgcgcgcgc gcacaaccgg acgcgcgccg
     1981 gcatcgaagc gcggctcgtc aagcttggca agctcgacga atcggccgtg accgtgcaac
     2041 tgcgctatcc gccgaaaccg agcggtcagg cccagcccgg ggcgtgaaaa aatagccgcc
     2101 ggcgcgggca cagcagtcag cgcgtagctc ggagcagcag tgacgcgccg atcagcacca
     2161 gcaacacgct gatcaggcgc agcagcgcgc cgcgcgaaat gcggccgtgg tagcggttgc
     2221 cgatccacag cccggtgagg gcgaacggca gcagcagcgc gaagccgatc acgcggtcgg
     2281 cgagcatgag gccgctcgcg agaaagacca gcgcgcggat cgatgtcgag agcagcacga
     2341 tgttcgaaag ggtcgcgcgc agcaccgt
//
LOCUS       DASURG010000405         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1297454, whole genome shotgun sequence.
ACCESSION   DASURG010000405 DASURG010000000
VERSION     DASURG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297454"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFY80_07140"
     CDS             complement(<1..1011)
                     /locus_tag="VFY80_07140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6007720.1"
                     /translation="MLFDLFFEVALPSHAGLSEAELYADTLASIEHADRLGYRCAWLV
                     EHHFMRGYSHASKPEVLLGAAAARTRRIRLGHGVIPLPLHHPVHVAERVATLDVVSSG
                     RLDVGIGRGFSPREYAVFGVPMEASRELTEESLEILRASFRRQPVSFHGRHFRLDALD
                     ILPHVVQEPHPPLWTAAVSPDTYAWAAAQELGVLAGPFKPWFMVKHDLARYLRAWRAA
                     APPRIGMTVGVLCLEDGARARALAEEAFTWYYGQLFTVTLPVLERLYPSYEQLHELGR
                     FRKLMKLGVQLGWLDTFGLTVVGNPRECTAKLQKYAKAGITHLVCAFGAGAVEHRVVE
                     ESL"
     gene            1148..1954
                     /locus_tag="VFY80_07145"
     CDS             1148..1954
                     /locus_tag="VFY80_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6007721.1"
                     /translation="MENLVEIRDVNFAYEVRPVLKGINMVVPRGKVVGIMGLSGCGKT
                     TLLRLIGGSLMPTGGEVRVAGQATHELDRDGLYALRRKMGMLFQFGALFTDMTTFDNV
                     AFQMREHTDLPESMIRDLVLMKLDAVGLRGAQHMMPSELSGGMARRVALARAVALDPM
                     LVMYDEPFAGLDPISLGVIGNLIRHLNDALGATSIVVTHDVQESLKIVDYIYFMSEGV
                     VVTHGTPDDIRNSKAPFVHQFVWGEMDGPVPFHYPGRGLPHELGFGDRHA"
     gene            1947..2741
                     /gene="mlaE"
                     /locus_tag="VFY80_07150"
     CDS             1947..2741
                     /gene="mlaE"
                     /locus_tag="VFY80_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX6007722.1"
                     /translation="MLERVAAPLRRLGHQCVNAVWRLGYASRFFVSVLLRSGMSFRRF
                     GLVIREIYFAGVLSLIIIIVSGLFVGMVLGLQGYETLQRYGSAEAVGTLVALSLTREL
                     GPVVAALLFASRAGSAMTAEIGLMKATEQISAMEMMAVDPVARVVGPRFWGGLISMPL
                     LAAMFSAIGVFGGYLIAVVLIGVDEGAFWSQMQASVDLRNDIVNGVIKSAVFGVTVTA
                     IALFEGYDAPPTAEGVSRATTRTVVTSSLAILGLDFVLTAFMFRGA"
     gene            2746..3222
                     /gene="mlaD"
                     /locus_tag="VFY80_07155"
     CDS             2746..3222
                     /gene="mlaD"
                     /locus_tag="VFY80_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX6007723.1"
                     /translation="MERSVLDLWVGIFVAGGIAALLILALKVGNASETFNVGDSYRVS
                     AEFDNIGGLKPRAPVKSAGVVVGRVTQIQFDNERFRARVLMNIDKRYQFPKDSSVSIL
                     TSGLLGEQYIGVEAGGDTENVQEGGTFKLTQSAVVLEKLIGQFMYSKAAEGGDAGK"
     gene            3257..>3543
                     /locus_tag="VFY80_07160"
     CDS             3257..>3543
                     /locus_tag="VFY80_07160"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6007724.1"
                     /translation="MKYRFLLTMLMLCVPVGAIQAAELGPDALAKSVTDEVLAIVRAD
                     KDVQSGDRKKVLEVVESKIVPHFNFARMTQLAMGRNWRQANAGQQKVLVDQ"
BASE COUNT          611 a         1068 c         1212 g          652 t
ORIGIN      
        1 gagcgactcc tcgaccaccc ggtgctccac cgcgccggca ccgaacgcgc acacaaggtg
       61 cgtgatgccg gctttcgcat acttttgcag cttcgcggtg cattcgcggg gattgccgac
      121 gaccgtgagg ccgaaggtgt cgagccagcc gagctggacg ccaagcttca tgagtttacg
      181 aaagcgcccc agctcgtgca gctgctcgta gctcggatag aggcgctcga gtaccggcag
      241 cgtcacggtg aaaagctgcc cgtagtacca ggtgaacgct tcctccgcca gcgcgcgcgc
      301 gcgcgcgccg tcttcgaggc atagcacgcc caccgtcatg ccgatgcgcg gcggtgcggc
      361 ggccctccac gctcggagat aacgcgccag gtcgtgcttc accatgaacc agggcttgaa
      421 cggccccgcg aggacgccga gctcctgcgc cgctgcccag gcgtaggtgt cgggactgac
      481 cgcggcggtc cagagtgggg gatgaggctc ctgcacgacg tgcggcagga tatcgagcgc
      541 atcgagcctg aaatgccggc cgtggaagct caccggttgc cgccggaagc ttgcgcgcaa
      601 aatctcgagg ctctcttcag tgagctcgcg gctcgcttcc atgggcacgc cgaacaccgc
      661 gtactcgcgc ggcgagaagc cgcggcctat gcccacatcc agccggccgg acgagaccac
      721 atcgagtgtc gccacgcgct cggccacatg cacgggatgg tgcaacggca gcggaatgac
      781 gccgtgcccc agccggatgc gccgggttcg cgcggcggct gcgccgagca ggacctcggg
      841 cttggaggca tgcgaatagc cgcgcatgaa gtgatgctcg acgagccacg cgcaacggta
      901 gccgaggcgg tccgcatgct cgatgctcgc gagcgtgtcg gcataaagct ctgcttcgga
      961 caggccggcg tgggaaggca gcgcgacttc gaagaacaga tcgaaaagca tgagagtgcg
     1021 atgatgccag cggggtcgac actttccaat caccgccacg ccggtttggg gcgctgcgtc
     1081 attggtgagg ggtgcggaag tggtttagaa ttagcgcttt ttaatcggcc gcggcgcccg
     1141 ctcggcgatg gaaaatctgg tcgaaatcag ggacgtcaat ttcgcgtacg aggtccgtcc
     1201 ggtactcaag ggcatcaaca tggtggtgcc ccgcggcaag gtggtcggca tcatggggct
     1261 gagcggctgc ggcaagacga cgctcctgcg cttgatcgga ggatcgctga tgccgaccgg
     1321 cggcgaggtg cgcgttgcgg gccaggcgac gcacgagctc gaccgcgacg gcctgtatgc
     1381 gctgcgccgc aagatgggga tgctgttcca gttcggcgcg ctcttcaccg acatgacgac
     1441 cttcgacaac gtcgcattcc agatgcgcga gcacaccgat cttcccgagt cgatgatccg
     1501 cgatctcgtg ctgatgaagc tcgatgcggt cggcctgcgc ggcgcgcagc acatgatgcc
     1561 gtccgagctt tccggcggga tggctcgccg ggtggcgctc gcgcgcgcgg tcgcgctcga
     1621 cccgatgctc gtcatgtacg acgagccctt cgccggcctc gatcccatct cgctcggcgt
     1681 gatcggtaat ctcatccgcc atctcaacga tgcgctgggt gcaacctcga tcgtcgtcac
     1741 acacgacgtt caggaatcgc tgaagatcgt cgattatatt tacttcatgt cggagggcgt
     1801 cgtggtgacg cacggcacgc cggacgacat ccgcaactcc aaggcgccat tcgtgcacca
     1861 gttcgtatgg ggggagatgg acggtccggt cccgttccac tatccgggga gaggacttcc
     1921 ccatgagctc ggcttcgggg atcgccatgc ttgagcgcgt tgcggcgccg ctgcggcgcc
     1981 tgggccacca gtgcgtcaat gcggtatggc ggttgggtta cgcgagccgc tttttcgttt
     2041 cggtgctgct gcgctcgggc atgagcttcc ggcgctttgg gctcgtcatc cgggagatct
     2101 atttcgcggg ggtgctctcc ctcatcatca tcatcgtttc gggcctgttc gtcggcatgg
     2161 tgctcgggct tcagggttac gaaaccttgc agcgctacgg ctctgccgaa gcggtgggga
     2221 cccttgtggc gttgtcgctt acccgtgagc tggggccggt ggtggcagcg cttctctttg
     2281 cgagccgcgc ggggtcggcg atgaccgccg agatcgggct catgaaggcg acggagcaga
     2341 tctccgcgat ggagatgatg gcggtcgatc ccgttgcacg agtcgtcgga ccgcgcttct
     2401 ggggcggact catctccatg ccgctcctcg cggcgatgtt cagcgcgatc ggggtgttcg
     2461 gcggttacct gatcgctgtc gtcctgatcg gggtcgatga aggggcattc tggtcgcaga
     2521 tgcaggcgtc cgtcgatctg cggaacgata tcgtgaatgg ggtgatcaag agcgcggtct
     2581 tcggcgtgac ggtgaccgcg atcgcgctct tcgaaggcta cgacgcgccg ccgaccgcgg
     2641 aaggcgtgtc gcgcgcaacg acccgcacgg tggtgacctc gtcgctcgcc atcctcggcc
     2701 ttgatttcgt tctcaccgcg tttatgttca gaggagcgtg agtccatgga acgcagcgtt
     2761 ctcgatttgt gggtagggat cttcgtggcc ggcggcatcg cggcgcttct gatactcgcg
     2821 ctcaaagtgg ggaacgcgag cgaaaccttc aacgtgggcg attcgtatcg cgtgtctgcg
     2881 gaattcgaca acatcggcgg gctcaagccg cgcgcgccgg tgaagagcgc cggtgtggtc
     2941 gtgggacgcg tgacccagat ccagttcgac aacgagcggt ttcgcgcgcg cgtcctgatg
     3001 aacatcgaca agcgctacca gtttcccaag gacagctcgg tgtccatact cacgtccgga
     3061 ctgctcggcg agcagtacat cggcgtggag gcgggcggag acacggaaaa cgtccaggag
     3121 ggcggaacgt tcaagctcac ccagtccgcg gtcgtgctcg aaaagcttat cgggcagttc
     3181 atgtacagca aggcggccga aggcggtgac gccggcaagt agccggagct gcatggcgga
     3241 tgatgaaagg aaagcgatga aataccggtt cttgttgacg atgctcatgt tgtgcgtccc
     3301 ggtcggggcg atacaggcgg cagagcttgg acccgatgca ttggcgaaga gcgtgaccga
     3361 tgaagtgctc gccatcgtcc gtgcggacaa ggacgtccag tccggcgatc gcaagaaggt
     3421 gctggaggtg gtcgaatcga agatcgtgcc gcacttcaac ttcgcgcgca tgacgcagct
     3481 cgccatgggc cgcaactggc ggcaggcgaa cgccggccaa cagaaggtcc tggtcgacca
     3541 gtt
//
LOCUS       DASURG010000406         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1195468, whole genome shotgun sequence.
ACCESSION   DASURG010000406 DASURG010000000
VERSION     DASURG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195468"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /locus_tag="VFY80_07165"
     CDS             <1..1868
                     /locus_tag="VFY80_07165"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6007725.1"
                     /translation="SASGAMDAILYELDDFSLLDRWVPILDRLARQHPDLITGEVEAR
                     AASSMFISMVMRQPHHRDLEYWVERAYRASRASSDANLRMSVEPLVAISIIWAGHYPK
                     AWAVIEGMQHLAQTAVVSPFQRTKLKTVETMYYMLTAMHEPCLKSMQEGLDIERTAGV
                     NVLSSQLLAYGAGGALGAGKLDVAEQLLRQLAELPATTPRFDRCLLHLFSAWAAVLSQ
                     DTLRAYQEQKLALRMAVEVGSPYFEVMCRLMSAQVLFACGEQRKAIEHFERVHDIARN
                     IRNHLLEFMTLVNYAHLAIEYGRPRSGMKALQYALELGKPRNYITFPCWNPTTMAVVC
                     AHALEGDIETDYVKSLIRRRALKPANAGVAAESWPWPFRIRTFGHFVLLKDDRPITFS
                     GKAQRRPLDLLKALIAYGGRRVREEQLTEALWPRIDGDSAHRSFTTTLHRLRKLLGED
                     RALLLQEGRLTLNAEYVWLDVWAFEQMSAEIDRLLKGPPDKVDAARLTRLADRILALY
                     TGPFLAGDTDEAWYISPRERLRNRFVRAMSDIAHYWQQSGQWERAVDYFQRSLEADHL
                     AEGFYRHLMLCYRQLGRNAEAIDVYQRCRKTFSAVLSVEPSRETTAIYERLVSAS"
     gene            2042..2416
                     /locus_tag="VFY80_07170"
     CDS             2042..2416
                     /locus_tag="VFY80_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007726.1"
                     /translation="MAATNLWAAWIGIFLGLASGTVQGLFFHREDWLGGYDAWPRRLM
                     RLGHISFFGIAFLNLAYANTVALLGGDADVPAASMLLVAGAITMPLVCYLAAWKKPLR
                     HLFPVPVLSLLGAVAALIGGLA"
     gene            2413..>3165
                     /locus_tag="VFY80_07175"
     CDS             2413..>3165
                     /locus_tag="VFY80_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX6007727.1"
                     /translation="MKIGLIAMSGIRACDEELLALGLTLPGFVERSQAIASLPSLGLL
                     TLAGMTPQRHQIVYIEVADIQRMPPVPRDFDLIALSSFSAQIYEAYALAQRYAAHGIP
                     VVMGGLHVTMNPHEPARYGASAVLGEGELVWSDVIQDAERGQLKPIYDARGKEFDLAQ
                     APMPAFELLDMYRYNRITVQTSRGCPWRCSFCASSILLTKRYKQKPAHKVLAEIDRIR
                     SLWPRPFIEFADDNSFVNRHYWRALLPELARRR"
BASE COUNT          570 a         1017 c          986 g          592 t
ORIGIN      
        1 tgagcgcatc gggcgcgatg gacgccatcc tctacgaact cgacgacttc tcgctcctag
       61 accgctgggt gcccatactc gaccgcctcg cgcgccagca tcccgatctc atcaccgggg
      121 aagtcgaagc gcgagcggcc tccagcatgt ttatctccat ggtgatgcgc cagccgcacc
      181 accgcgatct cgagtactgg gtggagcgcg cataccgggc ttcgcgcgct tcgtcagacg
      241 cgaacctgcg catgtccgtg gagccgctcg ttgcgatcag cattatctgg gcgggccatt
      301 acccgaaggc ctgggcggtg atcgagggca tgcagcattt ggcccagacc gcagtcgtgt
      361 cgccgtttca gcggacgaag ctgaagactg tcgaaaccat gtactacatg ctgacggcaa
      421 tgcacgagcc gtgcctgaaa tcgatgcagg aaggactcga cattgaacgg accgcgggcg
      481 tgaacgtgct gagctcgcag cttctggcat acggcgccgg cggggcgctc ggtgcaggaa
      541 agctcgatgt cgcagagcag ctcctgcgac agcttgccga attgcccgcg acgaccccgc
      601 gcttcgatcg ctgcctcctc cacctcttct cggcatgggc ggcggtgtta agccaggata
      661 cgctgcgcgc ctatcaggag cagaagcttg ccctgcgcat ggcggtcgag gtgggcagcc
      721 cgtatttcga agtgatgtgc cggctcatgt ccgcgcaagt gctgttcgcg tgcggcgaac
      781 agcgcaaggc gatcgagcac ttcgagcgcg tgcacgacat tgcgcgcaat atccgcaatc
      841 acctgctcga gttcatgaca ctcgtcaact atgcccacct cgcgatcgaa tacggccggc
      901 cccgttcggg catgaaggcg ctgcagtacg cgctggaact cgggaagccc cgcaattaca
      961 tcaccttccc ttgctggaat ccgaccacca tggcggtcgt ttgcgcccat gcgcttgaag
     1021 gcgacatcga gaccgactac gtgaaaagcc tcatccgtcg gcgcgcattg aagccggcaa
     1081 atgccggggt ggccgcggag agctggccgt ggccgttccg gatacgcaca ttcgggcact
     1141 tcgttctcct caaagacgac cggccgatca ccttcagcgg caaggcgcag cgccgaccac
     1201 ttgatctcct caaggcgctg atcgcctatg gcggccggcg cgtgcgcgag gagcaactga
     1261 ccgaagcgct ctggccccgc atcgacggcg attcggcgca cagatcgttc acgaccactt
     1321 tgcatcgtct gcgcaagttg ctgggcgagg atcgcgcact gctgcttcag gaagggcggc
     1381 tgaccttgaa cgcggaatac gtctggctcg atgtctgggc gttcgagcag atgagcgccg
     1441 agatcgaccg gctgctcaag ggcccgcctg acaaagtcga tgcggcgcgg ctcacacgcc
     1501 tcgctgatcg catcctcgcg ttgtataccg gccccttcct ggcgggcgat accgatgaag
     1561 cgtggtacat cagtccgcga gagcggctgc gcaaccgctt cgtgcgcgcg atgagcgaca
     1621 tcgcccatta ctggcagcag tctggacagt gggagcgcgc ggtcgattac ttccagcgca
     1681 gcctcgaggc cgatcacctc gcggagggct tctaccggca cctcatgctg tgttaccggc
     1741 agctcggccg gaacgctgaa gcgatcgacg tctatcagcg ctgccgcaag acgttttccg
     1801 cggttctcag tgtcgaacct tcacgcgaga cgacggcgat ctacgagagg ctcgtttcag
     1861 cgtcctaaat cacgcgcgca ctccaccgcc gcggccccgt tccgcatgcg cggccttgcg
     1921 ggccgctcgc acagactgcg ccatccgtaa gccaaccgta agctacccgt aatccaagtg
     1981 catacccgcg gctgacacac tgtccccgcc taaacgcaga atcgctttgg cggggagcaa
     2041 gatggcggcg acaaacctct gggcagcctg gatcggcatt tttctgggct tggcaagcgg
     2101 gactgtgcaa ggcctgttct ttcatcgtga ggactggctg gggggttatg acgcatggcc
     2161 ccgccgcctg atgcggctcg gtcacatcag cttcttcgga atcgccttcc tcaacctggc
     2221 ctatgcgaac acggtggcgc tcctcggtgg agatgccgac gtgccggcag cgtcgatgct
     2281 gctcgtcgca ggcgccatta ccatgccgct cgtgtgctat ctcgcagcgt ggaagaagcc
     2341 gctgcgacat ttgtttccgg ttccagtgct gagtctgctc ggtgcggtcg cggcattgat
     2401 cggcggcctc gcatgaagat cggcttgatc gcgatgagcg gaatccgggc gtgcgacgag
     2461 gagctgcttg cgctcggcct gacgttgccg ggcttcgtcg agcgctcgca agccatcgct
     2521 tccttgccga gcctcggcct gctcacgctc gcgggaatga cgccacagcg gcaccagatc
     2581 gtttacattg aagtcgccga tatccaacgc atgccgcccg tcccgcggga tttcgacctg
     2641 atcgcgctct cgagcttcag tgcgcagatc tacgaagcct atgcgctcgc ccagcgctac
     2701 gccgctcatg gcatcccggt tgtgatgggc ggcctgcacg tgacgatgaa cccgcacgag
     2761 ccggcgcgat acggggcaag tgccgtcctg ggcgagggag agttggtctg gagtgacgtc
     2821 atccaggacg ccgaacgtgg gcaattaaaa ccgatctacg atgcgcgtgg gaaggaattc
     2881 gatctggccc aggcgccgat gccggcgttc gagctgctgg acatgtatcg ctacaaccgc
     2941 atcacggtac agacgagccg cggctgtccc tggcgctgca gcttctgcgc gagctccatt
     3001 ctgctgacca agcgctacaa gcaaaagccg gcgcataaag tgctcgcgga aatcgacaga
     3061 atacgatcgc tgtggcctcg tccgttcatc gaattcgcgg acgacaattc cttcgtgaat
     3121 cggcactatt ggcgcgcgtt gctgcccgag ctcgcgcggc ggcgc
//
LOCUS       DASURG010000407         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_277563, whole genome shotgun sequence.
ACCESSION   DASURG010000407 DASURG010000000
VERSION     DASURG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277563"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VFY80_07180"
     CDS             complement(<1..1173)
                     /locus_tag="VFY80_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6007728.1"
                     /translation="MLKKRTGWLGTMVPAMCAAMGIGGGDAYAAEELRIGFLAPMTGA
                     FAQIGRDMANGFQMYVDEVKGDFAGAKVTFILEDEEGKPPVGVRKAEKLIRQDKVHVF
                     IGGLLASTGYALAPVSTRLKVTYIPSVAAADDLTQRDSAKYPYVVRTGWTSSQPSHPF
                     GEWACEQGYKRIAIVAADYAFGYEVAGGFQKSFEDCGGRVVQKIWPPLGTNDFGPYIP
                     TLKKDVDAIFTVMVGPMALQFPKQLAAAGIKKPVIGGGTSYDEFVLPSMGDEVIGHVS
                     ALQYSAAIDTPKNAAFVKKYREKYGKVPSYYSETNYTTAQMIHEVLKKTKGKWPGQEK
                     FIKMLSSLQLETVRGPVRFDELRNPVQNIYIKKVEKKKMFGYEKDELWNTVVKTYPN"
     gene            complement(1203..2201)
                     /locus_tag="VFY80_07185"
     CDS             complement(1203..2201)
                     /locus_tag="VFY80_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6007729.1"
                     /translation="MKPHQRLLVVVAAVLLALAASAPFASPYVIILITHALIFAILAM
                     SVDLLLGYTGLPSLGQAAYLGMGAYLTAILATKFQLGLDGTFWIIVVLGMLIGAATAA
                     FFGLFAIRATGVYFLMITLALGMCVWGLAYRWNSVTGGDNGIVLPPRPQFWGIDLAND
                     LVYFYLVFAFFVAALAALYLLVQSPFGRSLVGIRENELRMRILGYNTWLHKYIAFVIA
                     GAFGGVAGVLWAHSNGLVSPEDVILATSVDALLMVVLGGPGTLVGGAIGASVVVFLRE
                     YLSTLVPWWQYVLGAVYVLTILYLPGGLMGLPERMRRWRESTPPAKEAKEVQSVSH"
     gene            complement(2209..3069)
                     /locus_tag="VFY80_07190"
     CDS             complement(2209..3069)
                     /locus_tag="VFY80_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6007730.1"
                     /translation="MDLQFWVIQAFNGISYGALLFLLASGLTLIFGVMRIVNLAHGAY
                     FLLGGYVALSVIWTTGSWLLALPLAALAIAAVGLVMERVFLRPLGFDPLRQVLLTIGF
                     AFLFQQAALDIWGGDHLDIQPPDVLKESIVIGGLYFPVYRMFMIGMAIAIGLGLWLAL
                     DRTRMGAVVRAAVDDAEMARGVGIDTSRVSMFIFALGAFLAALGGVIGGAFLGVYPGL
                     DFVILPIAFAVVIIGGMGSLGGAAIGSLLVGLADNFGKALFPEISYFTLYAPMVLILA
                     LKPTGLFGRE"
     gene            3288..3611
                     /locus_tag="VFY80_07195"
     CDS             3288..3611
                     /locus_tag="VFY80_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007731.1"
                     /translation="MAGSTHDGHYEAVWPRTARQAKQKALAPRLATLEGKTIAQLWDY
                     VFRGDEVFEFLEEGLKARFPGIRFVSWRDFGNTHGSEERAVVAALPQRLRELGADAVV
                     SGMGC"
     gene            3633..>4808
                     /locus_tag="VFY80_07200"
     CDS             3633..>4808
                     /locus_tag="VFY80_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX6007732.1"
                     /translation="MRASAAAEGAGVPSSTLVCEGFLGLAGAASIGLGFANMPVARVV
                     GHPGVQSRETLRKNTLDVTLERVIDNLLHAPPAAKAAADPGPREVIARGSFEEINEYF
                     YDNGLSDGLPVVPPTIERVERFLCYTDRDPEESLGTLLPDSRAATVWSIAVNGVMAGC
                     RPEYMPVLVALIEAMADPAYGVEHSGNTPGGETLIILNGPIIHDLGFNYTQGVMRDGF
                     RPNTSIGRFWRLYLRNVAGFLPHKNDKSTFGNTWRVVVAENEEVVRRIGWTPNSADMG
                     CTAGDNTVTIARYTGGNHISSVSGSTPEEVMPYVCDAMVKQYSWQLMFTVGQGMGTLR
                     PLMLVSPIIAETIAGWGWSKQDLKRHLFEHARITAREFERILRDWTQKPIWNLTEEAK
                     "
BASE COUNT          937 a         1529 c         1536 g          806 t
ORIGIN      
        1 gttgggatac gtcttgacga ccgtattcca cagttcgtcc ttctcgtaac cgaacatctt
       61 cttcttctca actttcttga tgtagatgtt ctggaccgga ttgcgcagct cgtcgaaccg
      121 caccgggccg cgcaccgttt cgagctgcag actcgagagc atcttgatga atttctcctg
      181 gcccggccac ttgcccttcg tcttcttgag cacctcgtga atcatctgcg cggtggtgta
      241 gttggtctcc gagtagtacg acggcacctt gccgtatttc tcgcgatact tcttcacgaa
      301 ggccgcgttc ttcggcgtgt cgatcgccgc gctgtactgc agcgcggaca catgcccgat
      361 gacttcgtct cccatcgaag gcagcacgaa ctcgtcgtag ctcgtgccgc cgccgatcac
      421 cggcttcttg attccggctg cagcgagctg cttcgggaac tgcagcgcca tcggcccgac
      481 catcacggtg aagatggcat ccacgtcttt cttgagcgtc gggatgtacg ggccgaaatc
      541 gttcgtcccg agcggcggcc agatcttctg gacgacgcgc ccaccgcaat cctcgaacga
      601 cttctggaag ccgccggcga cttcgtatcc gaaggcatag tccgccgcga cgatcgcgat
      661 gcgcttgtag ccctgctcgc acgcccattc gccgaacgga tggctgggtt gcgagctcgt
      721 ccagccggtc cgcacgacat agggatactt ggcgctgtcg cgctgcgtca ggtcgtcggc
      781 agcggcgacg gacggaatat aggtgacttt cagtcgcgtg ctgaccggtg cgagcgcgta
      841 tccggtcgaa gcgagcagcc cgccgatgaa cacatggacc ttgtcctgac ggatcagctt
      901 ctccgccttg cgcacgccga ccggcggctt gccttcctcg tcttcgagaa tgaaggttac
      961 tttggcgccc gcgaagtcgc ctttgacttc gtcgacgtac atctgaaagc cgttcgccat
     1021 gtcgcgcccg atctgcgcga aggcgcccgt catcggcgca agaaaaccga tgcgcagttc
     1081 ctcggcggcg tacgcatcgc cgccgccgat tcccatcgcg gcgcacatcg ccggcaccat
     1141 cgttcccagc catccagttc gttttttcaa catgggttcc tcctttggct acaaagactc
     1201 gttcagtgag agaccgactg tacttccttc gcttccttcg cgggcggtgt cgattcgcgc
     1261 cagcgccgca tgcgttcggg caggcccatc agcccgccgg gcagatagag aatcgtaagg
     1321 acatagaccg cgccgaggac atactgccac cacgggacga gcgtactcag gtattcgcgc
     1381 aggaacacga ccaccgaagc gccgatcgcg ccacccacca gcgtgccagg gccgcccagc
     1441 accaccatga gcagtgcatc gacggaagtg gcgaggatca cgtcttccgg gctcacgagg
     1501 ccgttggagt gcgcccacag cacgcccgcg acgccgccga aagcgccggc gatgacgaac
     1561 gcgatgtact tgtggagcca ggtgttgtag ccgaggatgc gcatgcgcag ctcgttttcc
     1621 cggatgccga cgaggctgcg gccgaacgga gactgcacca gcaggtagag cgccgccagc
     1681 gcggcgacga agaacgcgaa cacgaggtag aagtagacaa ggtcgttcgc gagatcgata
     1741 ccccagaatt gcgggcgcgg cggcagcacg atgccgttgt ccccacccgt gacggaattc
     1801 cagcggtagg cgagacccca cacacacatc ccgagcgcga gcgtgatcat caggaaatag
     1861 acgccggtcg cacggatcgc gaagagcccg aaaaacgctg cggtcgcggc tccaatcagc
     1921 atgccgagca cgacgatgat ccagaaggtg ccgtcgagtc cgagctggaa cttggtcgcg
     1981 agaatcgcgg tgagataggc tcccatcccg agataggcgg cctgacccag cgaagggaga
     2041 ccggtatagc cgagcagcag atccacgctc atcgcgagta tcgcgaagat gagcgcgtgc
     2101 gtgatgagga tgatgacgta ggggctcgcg aacggcgcgc ttgccgcaag cgcgaggagg
     2161 accgcggcaa ccacaacaag gaggcgttga tggggcttca tgcgcgcttc actccctccc
     2221 gaagagcccc gtcggcttga gcgcaaggat gagcaccatc ggcgcgtaga gagtgaaata
     2281 ggagatttcg ggaaagagcg ccttgccgaa attgtccgcg aggccgacga gcaggctgcc
     2341 gattgctgcg ccgccgagac tgcccatccc gccgatgatg accaccgcga aggcgatcgg
     2401 caggatcacg aaatcgagcc cgggatagac gcccagaaag gcgccgccga tcacgccgcc
     2461 gagcgcagcg agaaatgcgc cgagcgcaaa gatgaacatc gagacgcgcg atgtatcgat
     2521 gccgacgccg cgcgccattt ccgcgtcgtc tactgccgcg cgcacgaccg cacccatgcg
     2581 cgtgcggtcg agcgcaagcc acaatccgag cccgatcgcg atggccatgc cgatcatgaa
     2641 catgcggtac accggaaagt agagcccgcc gatgacgatg ctttccttca gcacgtcggg
     2701 cggctggatg tcgaggtggt cgccccccca gatgtcgagc gccgcctgct ggaagagaaa
     2761 ggcgaagcca atggtgagca acacttggcg cagcgggtcg aacccgaggg ggcgcaggaa
     2821 cacgcgttcc atcacgaggc ccaccgccgc gatcgccaat gcggcgagcg gcagggcgag
     2881 gagccacgac ccggtcgtcc agatcacgga cagtgcgacg tagccgccca gcagaaagta
     2941 agcgccgtgc gcgagattga cgatgcgcat cacgccaaag atcagcgtga gcccgctcgc
     3001 cagcagaaaa aggagcgcgc cgtacgaaat cccgttgaac gcctgtatca cccagaactg
     3061 caggtccacc gcgccactcc tccttgcccc gcggaccgct tgcggccgcc ggcgcgtcat
     3121 gtcggtctat attaactgcc gtcgcttctt caaccaaatc cggcagcgac gcattgcacg
     3181 atcgccatcg cgcccggcag cctagcacga agcgcgcgat gcgtggtgcg cggcgcagcg
     3241 gcgtggggta tcctcgcgct agacgtccgg gcgagaggat tatcgagatg gcaggcagca
     3301 cgcacgacgg acattacgaa gccgtctggc cgcgcaccgc gcgccaggcg aaacagaaag
     3361 cgcttgcgcc gcggcttgca acgctcgaag ggaagaccat cgcgcagctg tgggactacg
     3421 tctttcgcgg cgacgaagtg ttcgaatttt tggaagaggg gctcaaggca cgctttcccg
     3481 gaatacggtt cgtgagctgg cgtgatttcg gaaacaccca cggaagcgag gagcgcgcgg
     3541 tcgtggctgc gctgccgcaa cggctacgcg agctcggcgc agacgcggtg gtttccggca
     3601 tgggctgttg aggcagctgc acgcccgccg tgttgcgggc gagtgccgcg gccgagggcg
     3661 ccggcgtgcc gtcctcgacg cttgtatgcg agggctttct cggcctcgcg ggcgctgcct
     3721 cgatcgggct cggcttcgcc aacatgccgg tcgcgcgtgt cgtcgggcat cccggcgtac
     3781 agagccgcga gacgctccgg aagaacacgc tcgatgtcac gctcgagcgg gtgatcgaca
     3841 acctcctcca tgcgccgcct gcggcaaagg ccgccgcaga ccccgggccc cgcgaggtga
     3901 tcgcgcgcgg gagcttcgag gaaatcaacg agtacttcta cgacaacggg ctttccgacg
     3961 gtctgccagt cgttccgccc acgatcgagc gggtggagcg attcctgtgc tataccgacc
     4021 gcgatcccga agaatcgctc ggaacgctgc tgcccgacag ccgggcagcc acggtgtgga
     4081 gcattgccgt caacggcgtc atggccggct gccgtccgga atacatgccg gtgctggtcg
     4141 cgctcatcga ggccatggcg gatcctgcct acggcgtgga gcacagcggc aacacgccgg
     4201 gcggcgagac cctcatcatt ctgaacggtc cgatcattca cgacttgggc ttcaactaca
     4261 cgcagggcgt gatgcgtgat ggctttcgtc cgaacacgtc gatcggccga ttctggcgcc
     4321 tgtacctgcg caacgttgcc ggattcctgc cccacaagaa cgacaagtcg acgttcggca
     4381 atacgtggcg cgtagtggtg gcggagaacg aggaggtggt gcgcaggatt ggttggacgc
     4441 cgaacagcgc cgacatgggt tgcacggcgg gcgacaacac agtgacgatc gcgcgctaca
     4501 ccggcggcaa ccacatttcc tcggtgtccg gaagcacgcc cgaagaagtg atgccctacg
     4561 tgtgcgatgc gatggtgaag cagtattcgt ggcagctcat gttcacggtg ggacagggca
     4621 tggggacgct ccgcccgctc atgctggtct cacccatcat cgccgagacg atcgccggct
     4681 ggggctggtc caagcaggat ctgaagcgcc atcttttcga gcatgcgcgg ataacggcgc
     4741 gcgaattcga gcgcatcctg cgcgactgga cgcagaagcc gatctggaat cttacggagg
     4801 aggcgaaa
//
LOCUS       DASURG010000408         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1503679, whole genome shotgun sequence.
ACCESSION   DASURG010000408 DASURG010000000
VERSION     DASURG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503679"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..697)
                     /gene="cynS"
                     /locus_tag="VFY80_07205"
     CDS             complement(254..697)
                     /gene="cynS"
                     /locus_tag="VFY80_07205"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX6007733.1"
                     /translation="MKRSDVTEMIVMARIKRGLTWARLAKAVGESKEWTTAALLGQMQ
                     MTKDQAEAAGKLLGLPEDAVLLLQQVPYKGSLPTAVPTDPLIYRFYELVNVYGTTFKE
                     LIHEEFGDGIMSAIDFRMDLKREPDPKGDRVKITMSGKYLPYKTY"
     gene            complement(778..1701)
                     /locus_tag="VFY80_07210"
     CDS             complement(778..1701)
                     /locus_tag="VFY80_07210"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6007734.1"
                     /translation="MTESLIKIEGITKRFTGRDGTATTVFEDLHFSIEAGEFVCLIGH
                     SGCGKTTILNVLAGLEPASAGYVLVGGREIDGPSLDRAVIFQSHALMPWLTVSGNIAF
                     AVSSRWPQWGRARVRAHCQEYIDLVNLTGSEKKRPSELSGGMKQRVGIARALAIRPKM
                     LLMDEPFSALDALTRGMLQEEVLRICAETRQTVFMITHDVDEAILLADKIILMTNGPH
                     AKIAEIVVNTLPRDRNRHDLHRHPHYYRIRNHLIEFLVDRSRAFVEAGAAAGYDPRKP
                     PLTRPGLEETVSQVAVPPAARTSRPVIAVAN"
     gene            complement(1750..>2050)
                     /locus_tag="VFY80_07215"
     CDS             complement(1750..>2050)
                     /locus_tag="VFY80_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6007735.1"
                     /translation="LNVARVLGLSRWQTVWRVMLPAALPMMFTGFRLSLGIAWLVIVA
                     AEMLVGGTGIGYFVWNEWNNLSITNILAAILAIGLVGMVLDLALARLTKMVTFPE"
BASE COUNT          360 a          646 c          636 g          408 t
ORIGIN      
        1 gcagttcagc gccatcgccg ccgaattgaa gcagtaaaac ggctcgcctg acctcgtcac
       61 tacggacttc cgtcattcca cttgcttccg tcattccggt gaaaaccgga atccaggcgc
      121 ctctctggat cccggcgttc gccgggatga cgttcgttgg tgcccgggat gacgttcgtt
      181 ggtgcgccgg gatgatgttc ggcgcgatcg caagagcgct caaatggagt ttcaggccgg
      241 cttccgtgcg gaactaatag gtcttgtacg gaagatactt cccgctcatc gtgattttca
      301 cgcggtcgcc tttcggatcg ggctcgcgct tgagatccat cctgaaatct atggcgctca
      361 tgatgccgtc tccgaactct tcgtgaatca gctccttgaa tgtggtgccg tacacgttga
      421 cgagctcgta gaagcggtag atcagcgggt cggtcgggac cgcggtcggc agcgacccct
      481 tgtacggcac ttgctggagg agcagcacgg cgtcttcggg caggcccaga agctttcccg
      541 cagcctccgc ctgatccttg gtcatctgca tctggccgag gagcgctgcg gtcgtccatt
      601 ccttgctctc gccgaccgcc ttcgcaagcc tggcccatgt gaggcctctc ttgatccgtg
      661 ccatcacgat catctctgtg acatcgctcc gcttcagcgg cttggcaaag ctggctgcag
      721 cgagcagagc ttccggtgca tcgcttcgtt tcatcgatcg tctccttggc tggggtttca
      781 gttggcaaca gcgattacag gtctcgacgt gcgcgcggcc gggggaaccg ctacctggga
      841 cacggtttcc tcgagcccgg gacgcgtgag cggcggctta cgcgggtcat atcccgccgc
      901 cgcgcccgcc tccacgaacg cacgcgatcg atcgaccaga aactcgatca ggtgattgcg
      961 gatgcggtag tagtgcggat gccggtgcag gtcatgtcgg ttgcggtcgc gcggcagcgt
     1021 gttcaccacg atttcggcaa tcttcgcgtg cggaccgttg gtcatgagaa tgatcttgtc
     1081 ggcgaggagg atcgcctcat cgacgtcgtg cgtgatcatg aacacggtct gccgcgtctc
     1141 ggcgcagatg cgcagcacct cttcctgcag catgccgcgc gtaagtgcat cgagcgcgga
     1201 gaacggctcg tccatcagca gcatcttcgg tcggatcgcg agcgcccggg cgatcccgac
     1261 gcgctgcttc atgccgccgg acagctcgga cggccgcttc ttctcggaac ccgtcagatt
     1321 gacgagatcg atgtactcct ggcagtgcgc gcgcacgcgc gccctgcccc actgcggcca
     1381 gcgcgaggac acggcaaaag cgatgttccc cgacactgtg agccagggca tcagcgcgtg
     1441 gctctggaaa atcaccgcgc ggtcgaggct cggaccatcg atctcccggc cgccgaccag
     1501 gacgtaaccg gcggaagcgg gctcgagtcc cgccagcacg ttgagtatcg tcgtcttgcc
     1561 gcagccggag tggccgatga ggcaaacgaa ttcgcccgct tcgatgctga agtgcagatc
     1621 ctcgaagacc gtggtggccg tcccgtcgcg gccggtgaag cgcttggtga tgccttcaat
     1681 cttgatcaga gattcggtca ttcgcaaacg cgtgattcgc tatggatcgt tctggcggac
     1741 atgtctcatt cactccggga atgtcaccat cttcgtgagc cgggcaagcg ccagatcaag
     1801 caccatgccg accaggccga tcgcgagaat cgcggcgagg atgttagtga tcgagagatt
     1861 gttccattcg ttccacacga aatagccgat gccggtgccg cccacgagca tctcggccgc
     1921 gacgatcacg agccacgcga tgccgaggct caggcggaag ccggtgaaca tcatcggcag
     1981 cgctgccggc agcatcacgc gccacaccgt ctgccagcgc gacagcccga ggacgcgcgc
     2041 gacgttcagg
//
LOCUS       DASURG010000409         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_583078, whole genome shotgun sequence.
ACCESSION   DASURG010000409 DASURG010000000
VERSION     DASURG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583078"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..808
                     /gene="rph"
                     /locus_tag="VFY80_07220"
     CDS             80..808
                     /gene="rph"
                     /locus_tag="VFY80_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6007736.1"
                     /translation="MTTPMRPSGRQPGELRAVRITRHYTRHAEGSVLIEFGDTKVICT
                     ASVLERQPPHLKGTSQGWVTAEYGMLPRSTGTRTDREAARGKQSGRTQEIQRLIGRAL
                     RAVVALDKVGERTIQLDCDVIQADGGTRTASITGAFVALHDAVEGLIARGALNASPIR
                     DFVAGVSVGMYAGTPLLDLDYLEDSQCETDMNVVMTGSGGLVEIQGTAEGAPFSRAAM
                     EEMVDLAERGIRQLVAAQRASLNA"
     gene            837..1469
                     /gene="rdgB"
                     /locus_tag="VFY80_07225"
     CDS             837..1469
                     /gene="rdgB"
                     /locus_tag="VFY80_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX6007737.1"
                     /translation="MKKIVIASGNPGKLREFGEMLRPLGMEVMAQAELGINEIDEPHT
                     TFVENALAKARHASRLSDLPALADDSGICVAALGGEPGVNSARFAGPPPSGIRASREE
                     QDRRNNEKMIGLLAGVHDRRAHYYCVIVLMRHGEDPEPLIAEGRWHGTIIDTPRGANG
                     FGYDPYFLLPALGLTAAELAPAEKNAASHRGRALAALLARLRPASTEECT"
     gene            1466..>2627
                     /gene="hemW"
                     /locus_tag="VFY80_07230"
     CDS             1466..>2627
                     /gene="hemW"
                     /locus_tag="VFY80_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX6007738.1"
                     /translation="MTSAASVVQAKITPRLTALPPLALYIHIPWCVRKCPYCDFNSHE
                     LRGALPEREYIAALVSDLEHALPQVWSRRVSSVFFGGGTPSVLSAQAIDAILSNVRAR
                     LPIAADAEITLEANPGTFEAARFRAYHATGVNRLSIGIQSFNRGLLQALGRIHDDAEA
                     RRAMEIARGTFDNVNLDLMYALPQQTVEEACADIETAVQYAPSHLSAYHLTIEPNTYF
                     HRYPPRLPTEDISAAMQEEIEARLAVHGYTQYEVSAFAKSRDLACRHNLNYWTFGDYL
                     GIGAGAHSKISSATGIVREMRYKQPKLYMESAARGVPVQSRQELTGAEAAFEFMMNAL
                     RLTSGFEARLFGERTGVPLTEVLPALDEAERRGLLERDHRRIAPTALGRRFLN"
BASE COUNT          476 a          865 c          848 g          438 t
ORIGIN      
        1 cgcatcgttc accgtttacc attgtcgctc gaaatcggag ggccgcggcc acgatttcgg
       61 ccacccaacg atagcacgaa tgacgacccc aatgcgaccc agcggccggc aacccggaga
      121 attgcgcgcg gtgcgtatca cgcgccacta cacccgtcac gccgagggct cggtgctcat
      181 cgaattcggt gacacgaagg tgatctgcac cgcaagcgtg ctcgaacgcc agccgccgca
      241 cctcaaaggc acatcgcagg gctgggtgac tgcggaatac ggcatgctgc cgcgctccac
      301 cggcacgcgg accgaccgcg aggccgcacg cggcaaacag tcggggcgga cgcaggagat
      361 tcagcgcctc atcggtcggg cgctgcgcgc agtggtcgcg ctcgacaagg tgggcgagcg
      421 caccattcag ctcgactgcg atgtgatcca ggccgatggc ggcacacgca ccgccagcat
      481 caccggtgcc ttcgtggcct tgcacgacgc cgtggagggg ctcattgcgc gcggtgcgct
      541 gaacgcatcg ccgatacgcg acttcgtggc cggcgtgtcg gtcggcatgt acgccggaac
      601 gccactgctg gatctcgact atctcgaaga ctcgcagtgc gagacggaca tgaatgtcgt
      661 catgaccggt tccggcggtt tggtggagat tcagggcacg gccgaagggg cgcctttttc
      721 gcgcgccgcg atggaggaga tggtggatct cgccgaacgc gggatccggc agctcgttgc
      781 agcccagcgc gcgagcctca acgcctgaac tcgtcaagcg cacctttgtc tgcccgatga
      841 agaagatcgt catcgcgtcg ggtaatccgg gcaagctccg cgaattcggc gagatgcttc
      901 gcccgcttgg aatggaagtg atggcgcagg ccgagctcgg cataaacgaa atcgacgagc
      961 cacacacgac cttcgtcgag aacgcgctcg ccaaggcgcg ccatgcgagc aggctttccg
     1021 atctgccggc gcttgcggac gattcgggca tatgcgtggc ggcgctcggc ggagagccgg
     1081 gcgtaaattc cgcgcggttc gcgggccccc caccgtccgg catccgggcg agccgcgaag
     1141 agcaggaccg ccgcaacaac gagaaaatga tcggcctgct cgcgggcgtg cacgaccggc
     1201 gcgcgcacta ttactgcgtg atcgttctca tgcgccacgg cgaggatccc gagccgctca
     1261 tcgccgaagg gcgctggcac ggaacgatca tcgatacgcc gcgcggcgcg aatggctttg
     1321 gctatgaccc ctatttcctc ttgccggcgc tcgggctgac cgcggccgaa ctcgcgcccg
     1381 ccgaaaagaa cgcggcaagc catcgcggcc gggccttggc cgcgctgctc gcccggctgc
     1441 ggcctgcgag cacggaggaa tgcacatgac gagtgccgca tccgtcgttc aagccaaaat
     1501 tacgccgcgg ctcacggcgc tgccgccgct tgcgctctac attcatattc cgtggtgcgt
     1561 gcgcaagtgt ccctattgcg acttcaattc ccacgagctg cgcggcgcgc ttccggagcg
     1621 cgagtacatc gctgcgctcg tctcggacct ggagcacgcg ctaccgcaag tctggagccg
     1681 gcgggtgagc agcgttttct tcgggggcgg caccccgagc gtgctctcgg cgcaggcgat
     1741 cgacgcgatt ctttcgaatg tgcgcgcccg gcttccgatc gcggccgacg cggaaatcac
     1801 gctcgaagcg aatcccggca cgttcgaggc cgcgcggttc agggcctatc acgcgaccgg
     1861 cgtgaatcgg ctctcgatcg ggatccagag cttcaatcgg ggtctcctcc aggcactggg
     1921 acgcatccat gacgacgccg aggcgagacg cgcgatggag atcgcgcgcg ggacgttcga
     1981 caatgtgaac ctcgacctca tgtacgcgct gccgcagcag acggtggaag aggcgtgcgc
     2041 cgacatcgaa actgcggttc agtatgcgcc ctcgcacctc tcggcatacc atcttacgat
     2101 cgagcccaac acctactttc accgctaccc tccgcgactg cccactgaag acatttccgc
     2161 cgcgatgcag gaggaaatcg aagcgcgcct tgcggtgcac ggctataccc agtacgaagt
     2221 ttcggccttt gcgaagtcac gcgatttagc gtgccgccac aatctcaact actggacctt
     2281 cggcgactat ctgggaatag gcgccggtgc gcacagcaag atctcttccg caaccggcat
     2341 cgtgcgcgag atgcgttaca agcagccgaa gctgtacatg gaaagcgccg cgcgcggtgt
     2401 tcctgttcag tcccggcagg agctgaccgg agcggaagcg gccttcgagt tcatgatgaa
     2461 tgcgctgcgg cttacttccg gattcgaggc gcggctattc ggcgagcgca cgggcgttcc
     2521 actcactgag gtgttgcccg cgctggacga agcggagcgg cgtgggctcc tcgagcgcga
     2581 ccatcggcgc attgcaccta cggctctcgg ccggcgcttc ctcaacg
//
LOCUS       DASURG010000410         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1503828, whole genome shotgun sequence.
ACCESSION   DASURG010000410 DASURG010000000
VERSION     DASURG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503828"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2517
                     /gene="acnA"
                     /locus_tag="VFY80_07235"
     CDS             <1..2517
                     /gene="acnA"
                     /locus_tag="VFY80_07235"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX6007739.1"
                     /translation="KKITEGHLKTLAGWKPQASRTEEVPLVVARVLLQDMTGVPLVVD
                     FAAMREAAKAQGKDPEIIEPIVPGHLIMDHSVQIDHYGSPDALRKNMEIEFKRNRERY
                     QFLKWSAQAFKTFHIIPPGNGICHQINLEYVSRVVWERDGVYYPDSLVGIDSHTTMVN
                     GLGVLGWGVGGIEAEAAMLGQPMYFLEPDVVGVHLTGRLRDGVTATDLVLRITQLLRE
                     KKVVGKFVEYFGEGAASLPVPDRATIGNMSPEYGATCGYFPVDDVACEFLRMTGRGDV
                     VPAVQAYYKAQGMYGMPKKGDIDYSEVVELDLGTVKAAVSGPRRPHDRLDLDALKGKY
                     NELMQKPFVDGGYGKANEMEKRVPTGKSGVDVGHGDVLISAITSCTNTSNPGVLLGAG
                     LLAKKAAEKGLKPHPRVKTSLGPGSLAVTEYLKNAGLLTYLEQLGYYVAGYGCTTCIG
                     NSGPLDAQIEDSVAKNDIVAAAVLSGNRNFEARIHQNIRANFLMSPPLVVAFGLAGTV
                     NIDLTRDPLGTGKDGKPVYLRDIWPSTQEIAAVMKYATDPEMYRKLFSAFGEGNPMWD
                     EIPASVGATYQWDDKSTYVRLPPYFEGFSMQPGKVGDIRGARVLGIFGDSITTDHISP
                     AGGIRATTPAGKYLQEHGVTVADFNTYGARRGNHEVMMRGTFANVRIKNLMVPGTEGG
                     ITVHQPSGEQMPIYEAAMRYKKDGVPSVVFGGDEYGQGSSRDWAAKGPQLLGVKAVIV
                     RNFERIHRANLVFMGVLPLQFKDGASVQSLKIDGSETIDLQGIENGITPMQDVTMVVR
                     RKNGQTDKVPLQLRIDTPIEVDYYKHGGILPYVLRELLAA"
BASE COUNT          520 a          846 c          846 g          434 t
ORIGIN      
        1 aagaaaatta cggaaggcca tttgaaaacg ctcgcgggat ggaagccgca ggcgtcgcgc
       61 accgaggaag tgccgctcgt cgtcgcgcga gtcctgctgc aggacatgac cggtgtcccg
      121 ctggtcgtcg atttcgccgc aatgcgtgaa gcggcaaagg ctcagggcaa ggacccggag
      181 atcatcgagc cgatcgtgcc gggccacctc atcatggatc actcggtgca gatcgaccac
      241 tacggcagcc cggacgcgct caggaagaac atggagatcg agttcaagcg caaccgcgag
      301 cgctaccagt tcctcaaatg gagcgcgcag gcgttcaaga ccttccatat cattccgccc
      361 ggcaacggca tctgccacca gatcaacctc gagtacgtct cgcgcgtcgt gtgggagcgg
      421 gacggcgtct actaccccga cagcctggtc ggcatcgact cgcacacgac gatggtgaac
      481 gggctcggcg tgctcggttg gggcgtcggc ggtatcgaag ccgaagcggc gatgctcggc
      541 cagccgatgt acttcctcga gcctgacgtc gtgggcgtgc acctgaccgg ccggctcagg
      601 gacggtgtca cggcgaccga cctcgtgctc cggatcacgc agctcctgcg cgaaaagaaa
      661 gtggtgggca agttcgtcga atacttcggc gagggcgcag cctcgcttcc cgttccggat
      721 cgcgcgacca tcggcaacat gtcgccggaa tacggggcga cctgcggcta cttccccgtc
      781 gatgacgtgg cgtgcgaatt cctgcgcatg acgggacgcg gcgacgtcgt gccggcggtc
      841 caggcctatt acaaggcgca gggcatgtac ggcatgccga agaaaggcga catcgactac
      901 agcgaggtcg tggagctcga tctcggtacc gtcaaggccg ccgtctccgg cccgcgccgt
      961 ccgcacgacc ggctcgatct cgacgcgctc aagggcaagt acaacgagct catgcagaag
     1021 cccttcgtcg acggtggcta tggcaaggca aacgaaatgg agaagcgcgt gccgaccgga
     1081 aagtccggcg ttgacgttgg ccacggcgac gtgctgatct ccgcgatcac ctcgtgcacc
     1141 aacacctcga accctggcgt gctgctgggt gcaggcttgc ttgcaaagaa agcggcagag
     1201 aaaggactga agccccatcc gcgcgtgaaa acgtcacttg ggcccggctc gctcgcggtg
     1261 accgaatatc tgaagaacgc cgggcttctc acctatctcg agcagctcgg ctattacgtg
     1321 gcgggctacg gctgcacgac ctgcatcggc aactccggcc cgctcgatgc gcagatcgaa
     1381 gactcggtcg cgaagaacga tatcgttgcg gccgccgtgc tttcgggaaa ccgcaatttc
     1441 gaagcgcgca tccaccagaa catccgcgcg aatttcctga tgagcccgcc gctcgttgtg
     1501 gcattcgggc tcgcgggcac ggtgaacatc gatctcaccc gggatccgct cggcacgggc
     1561 aaggacggca agccggttta ccttcgcgac atctggccct ccacgcagga gatcgcggcg
     1621 gtcatgaagt acgcgaccga tccggagatg tatcgcaagc tcttttccgc cttcggcgaa
     1681 ggcaacccga tgtgggacga aattccggcc tccgtcggcg caacgtatca gtgggacgac
     1741 aagtccacct acgtgcggct tccgccctat ttcgaaggct tctcgatgca gccgggcaag
     1801 gtgggcgaca tccggggcgc acgcgtgctg gggatcttcg gagattccat caccaccgac
     1861 cacatcagcc ccgcgggcgg cattcgcgcg acgacccccg cgggcaagta cctccaggag
     1921 cacggtgtga cggtggcgga cttcaacacc tacggcgcgc gacgcggcaa tcacgaagtc
     1981 atgatgcgcg gcacgttcgc caatgtgcgc atcaagaacc tgatggtgcc cggcacggaa
     2041 ggcggcatca ctgtccatca gccgtccggc gagcagatgc cgatctacga agccgcgatg
     2101 cgctacaaga aggacggcgt gccttcggtc gtgttcggcg gggacgagta cggccagggc
     2161 tcttcgcgcg actgggcggc gaagggtccg cagctcctcg gcgtgaaagc ggtgatcgtg
     2221 cgcaacttcg agcgcatcca ccgcgccaat ctcgtcttca tgggcgtgct cccgctccag
     2281 ttcaaggacg gtgcgagcgt gcagtcgctc aagatcgacg gctccgagac gatcgacctg
     2341 cagggcatcg aaaacggcat cacgccgatg caggacgtga cgatggtcgt acgccgcaag
     2401 aacggccaga ccgacaaggt gccgctgcag ctgcggatcg acacgccgat cgaggtcgat
     2461 tactacaagc acggcgggat cctgccctac gtactgcggg agctgctcgc cgcttgatca
     2521 ctttcccgcc ctccccgctt gggcgcggca gggcagacga atgaagacgg cgcccacccg
     2581 gcgtcgcctt tccacgcagg ccggcattca aaagatgccg gcctgtttct ttggcacacg
     2641 ggatgc
//
LOCUS       DASURG010000411         7068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_685854, whole genome shotgun sequence.
ACCESSION   DASURG010000411 DASURG010000000
VERSION     DASURG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685854"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..864)
                     /locus_tag="VFY80_07240"
     CDS             complement(1..864)
                     /locus_tag="VFY80_07240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6007740.1"
                     /translation="MSCSALRLLMWFAGLWVAIATNAYAAQPFWYFRNLDSPFVPTNQ
                     ATVDAMLRIANVNASDYVVDLGSGDGRILITAARRYGAHGLGVDLDPDLVKESLRNAR
                     MAGVADRVRFEQRDLFDTDVSKASVVTLYLLPEVNLKLRPRLLAQLRPGTRIVSHGFG
                     LGDWQPDLRLSVRGTGSEVLFWIVPAAVAGKWSGDIGGTEGASLEIEFKQNFQEVDGS
                     ARRGGKLVHLRDARIEGDRLSFVLIDDADHTHRQLFEGQVREKLIEGTVRGSGSALRR
                     QQSWKASKLTP"
     gene            1135..1377
                     /locus_tag="VFY80_07245"
     CDS             1135..1377
                     /locus_tag="VFY80_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007741.1"
                     /translation="MVIDLEQYRLAKRRNTSAAAVNVRRYDEERLCVNWRPTSGLAAT
                     FCYREPHLMSPALPDNLDGIDVEDFVDRLSALASQI"
     gene            complement(1379..3517)
                     /locus_tag="VFY80_07250"
     CDS             complement(1379..3517)
                     /locus_tag="VFY80_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6007742.1"
                     /translation="MSPADKPVPRKLPGSLDGNRMLSRWLSINPDGTVTVYPGKVEIG
                     QGILTALTQIVAEELDVAIDRIKLAPASTAYSPNEGMTSGSRSIQESGMALRYAAAEV
                     KDLLLERAASRLGVSVEQLEIADGEVQARSGGRTSYWEVASEELLRREATPDMVAKVP
                     AEHTIVGASIPRRDLPAKVTGVPAYVHDMELPGMLHGRIVRPPSRKAKLVTLDDESEV
                     RGLPGVRALVRDGNFLGVIAEREEQAIRAQRRLARSARWQESAALPQSSDPRYLLAMP
                     SEDEVISEKSDETARARAARRFSAEYSRPLIAHASIGPSCAVAVFENGRYNVWTHSQG
                     IFPLRADLAKVLRVAESDIVVTHAEGAGCYGHNGADDVALDAALLARAIPGRPVRVQW
                     MREDEFQWEPYGSGMVIRMGAALDAKGDIVEWTHELWSHGHSTRPGPQDGANLLAAWH
                     LGEPVKMPRPGNPGLPAGGSHRNAIPLYTFPNQTVTNHLIREAPLRTSALRSLGGHVN
                     VFAIESFMDELAQAAGADPVEFRLRHLRDPRGRAVIEAVARRAQWRERERGDGVRGRG
                     IGFARYKNLGCYVAVIVEVEAEAEIRVKRAWAAVDVGQVISPDGLINQIEGGIVQTAS
                     WTLKEQVNFDRERVTTQTWGDYPILTFAETPEIEVEVLNRPGEPVLGAGEGAQGPTGA
                     AIANAVENALGVRLRDMPLTRDKLIAAIAA"
     gene            complement(3514..3978)
                     /locus_tag="VFY80_07255"
     CDS             complement(3514..3978)
                     /locus_tag="VFY80_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6007743.1"
                     /translation="MAITLNVNRAMRVVDAEPETPLLYVLRNELKLKGARFGCGAGHC
                     GACMVIVDGKAVPSCDVPVAAVAGKPITTIEGIASDARLHPLQEAFIGEQAAQCGYCA
                     TGIIMAAKALLDVNSQPSDEEIKAALANNLCRCGTHQRILRAIRRAVKGIAQ"
     gene            4138..4341
                     /locus_tag="VFY80_07260"
     CDS             4138..4341
                     /locus_tag="VFY80_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX6007744.1"
                     /translation="MILDVEALKERIHQLHIEHRDLDDVISRLSQSPAQDQLQLQRLK
                     KRKLYLKDQIALLERQLTPDIPA"
     gene            complement(4416..4925)
                     /locus_tag="VFY80_07265"
     CDS             complement(4416..4925)
                     /locus_tag="VFY80_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX6007745.1"
                     /translation="MDTRTTARIGYGAGSAICIALIAFAYYLQYVEYQEPCPLCILQR
                     VAFFGLGVVFLVAAIHGPGRVGSAIYGVLLTLIAGIGAAIATRHVWLQHLPKDRVPEC
                     GPGLEYMLRKFPLSQVLDKVLRGSGQCAEAGWTFLGLSIAGWSLVWFVLLGVFAAGLA
                     LYVRARAPR"
     gene            complement(4928..5422)
                     /locus_tag="VFY80_07270"
     CDS             complement(4928..5422)
                     /locus_tag="VFY80_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEX6007746.1"
                     /translation="MTDAPATTPFSAIEGSPLALAMRHELWLYPAVEILHIAGFVVLV
                     GSITMFDLRLLGLSRKLSVRLMARHLLPWTGGALIVIVPTGVLMFIAHASDFLVNPAF
                     QLKLMLILAAGVNAALFHAGVYRGVGLWDAEVAPPLPARLHAGTSLALWFGVIACGRL
                     LAYL"
     gene            complement(5419..5763)
                     /locus_tag="VFY80_07275"
     CDS             complement(5419..5763)
                     /locus_tag="VFY80_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX6007747.1"
                     /translation="MRSAVCLSVLLLASLPAHAHHGWSEYDSTRPLNLSGTIAESGYE
                     HPHGHIRLNTDNKTWWVVLAPPTRMANRGLPASALKKGARATVTGYPNRSKPEEMRAE
                     RITVDGKTVELR"
     gene            5830..6249
                     /gene="arfB"
                     /locus_tag="VFY80_07280"
     CDS             5830..6249
                     /gene="arfB"
                     /locus_tag="VFY80_07280"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX6007748.1"
                     /translation="MIRVTADIVLDESEIEERFIRASGPGGQNVNKVATAVQLRFNVL
                     RSKRLPEAVRARLITLAGRRLAGDGSLVITAERFRNQRRNRDDALDRLIALVRRAAEP
                     PKRRRATRPTAASRERRLEGKRRRGEIKRRRVRLDLI"
     gene            6373..6876
                     /locus_tag="VFY80_07285"
     CDS             6373..6876
                     /locus_tag="VFY80_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6007749.1"
                     /translation="MLRAAAVFRVTSAAVFASVMSVSTVYSHAASGEEVIKARIDFMK
                     DEMEGNWKPLAAFAKGKGSLADVEKHAAAISKRANDIPKHFPKDTGRGKYPDKLTRTL
                     PAVWEDWDGFKKDTQRLVEGSDRLARLAREGKKDEVLELIGPGGYGSTRIGCAECHDN
                     FRGPRAK"
BASE COUNT         1287 a         2349 c         2206 g         1226 t
ORIGIN      
        1 ctatggcgtg agcttgcttg ctttccagct ctgctgcctg cgcagcgcgc tgccgcttcc
       61 tcgcaccgtg ccttcgatca gcttttcgcg gacctggccc tcgaagagtt gtcgatgagt
      121 gtggtcggca tcgtcgatca gcacgaacga gaggcgatcg ccctcgatcc gggcatcgcg
      181 aagatggacg agcttgccgc cgcgacgagc actgccatcg acttcctgga agttctgctt
      241 gaattcgatc tccagcgacg cgccttccgt ccccccgatg tcgccgctcc actttcccgc
      301 caccgcggcg ggaacgatcc agaacaatac ctcgctgccg gtgccgcgca cgctgagcct
      361 caaatcgggc tgccagtcgc caagcccgaa gccgtgcgag acgatgcgcg tgccggggcg
      421 tagctgtgca agcagccggg ggcgcagctt cagattgacc tcgggcagca ggtacagcgt
      481 caccacgctc gctttgctga cgtccgtatc gaacagatcc cgttgctcga agcgcacgcg
      541 gtccgcgaca cccgccatgc gcgcgttgcg caggctctcc ttcacaagat cggggtcgag
      601 atctacgccc aggccgtgcg cgccgtagcg gcgtgccgcc gtgatgagga tgcgcccgtc
      661 tcccgagccg agatcgacga cgtagtcgct ggcattgacg ttcgcgatgc gcagcatggc
      721 gtccacggtg gcctggttgg tcgggacgaa aggagaatcg agattcctga agtaccagaa
      781 aggctgggca gcatacgcgt tcgtcgcgat agcaacccag agtccggcaa accacatcag
      841 caggcgcagg gcggagcagc tcatcgcgct catgcggggt ttcgcattcc ttgggtgaaa
      901 aatcgcggca cttgcgacaa gagtgtcgaa aaccggtacg tcagtgtcga atttcgtatc
      961 aacccaccgg tttcggaccc ggtgtttcat tctccggcgc cattccggtg tcgcgatcgt
     1021 ggtgccgcat caatgccttg gcccactcct gtgaattatt accggtctcg cgcattgtct
     1081 ggcacaaaac ctgcacaagc cttgtgccgg gaaaacaatt gctggaggac ggacatggtg
     1141 atcgatctcg aacagtatcg gctggcgaag cgcagaaaca cgtcagccgc ggcggtcaac
     1201 gtgcgccgct acgatgaaga acggctgtgc gtcaactgga ggccgacaag cggtctcgcg
     1261 gcgaccttct gctatcgcga gccgcacctc atgagcccgg cgctgcccga caacctcgat
     1321 gggatcgacg tcgaggactt cgtcgaccgc ctgagcgcgc tcgcctccca gatctgaatc
     1381 aagccgcaat cgcggcgatc agcttgtcgc gcgtgagcgg catgtcgcgg agccgcacgc
     1441 caagcgcgtt ctcgacggcg ttcgcgatcg ccgcgccggt cgggccttgt gcaccctcgc
     1501 ctgctcccag caccggctcg cccggccggt tcagcacttc gacctcgatc tccggtgtct
     1561 cggcgaaggt cagtatcgga taatcgcccc acgtctgcgt cgtgacgcgc tcacggtcga
     1621 agttcacctg ctccttgagc gtccagctcg cggtctggac gataccgcct tcaatctggt
     1681 tgatgagacc gtcgggggag atcacctggc ccacgtccac cgccgcccac gcgcgtttca
     1741 cccgtatttc ggcctccgcc tccacttcga cgatcaccgc gacatagcac ccgagattct
     1801 tgtagcgggc gaaaccgata ccgcgtccgc gcaccccgtc gccccgctca cgttcgcgcc
     1861 actgcgcccg gcgcgcaacc gcctcgatca cggcacgccc gcggggatcc cgcaggtggc
     1921 gcaggcggaa ctccaccgga tccgcgcccg ccgcctgcgc aagctcgtcc atgaacgact
     1981 cgatcgcgaa gacgttgacg tgcccgccga gcgaacgcag cgccgaggtg cggagcggcg
     2041 cctcgcgaat caggtgattc gtcaccgtct ggttgggaaa cgtatacagc ggaatcgcgt
     2101 tgcggtggct gccgccggca ggcagcccgg gatttccggg acgtggcatc ttcacgggct
     2161 caccaaggtg ccacgcggcg agcaggttcg ccccgtcctg cggaccgggc cgcgtgctgt
     2221 gtccgtggct ccacagctcg tgcgtccatt cgacgatatc gcccttcgcg tcgagagcgg
     2281 cgcccatccg aatcaccatt cccgagccat acggctccca ctggaattca tcctcccgca
     2341 tccactgcac gcggaccgga cgcccgggaa ttgcacgcgc gagcagggcc gcatcgagcg
     2401 ccacgtcatc ggcgccgtta tggccgtagc atcccgcgcc ttcggcatgc gtcaccacga
     2461 tgtcgctctc tgccacccgc agcacttttg cgagatcggc gcgcagcgga aagatgccct
     2521 ggctgtgcgt ccacacgttg tatctgccgt tctcgaatac ggcgacggca caggaaggac
     2581 cgatggatgc gtgggcgatg agcgggcggc tgtattcggc actgaatctg cgcgccgcgc
     2641 gggcacgtgc cgtctcatcg ctcttctcgc tgatcacctc gtcctccgaa ggcatggcga
     2701 gcaggtagcg cgggtcactg ctctgcggca gcgcggcgct ctcctgccag cgggcgctgc
     2761 gtgcgagccg ccgctgcgcg cgaatcgcct gctcctcgcg ctcggcgatg acaccgagga
     2821 aattgccgtc gcgcacaagc gcgcgcaccc ccggtaagcc gcgcacttcg ctttcgtcat
     2881 ccagcgtaac gagcttcgcc ttccggctcg gtggccgcac gatgcgcccg tgcagcatgc
     2941 ccgggagctc catgtcgtgc acgtaggccg gcacgccggt cactttcgcc ggaaggtcgc
     3001 ggcgcggaat ggatgccccg acgatcgtgt gctcggcggg caccttggcg accatgtcgg
     3061 gcgtcgcttc gcgtcgcagc agctcttcgc tcgccacttc ccagtagctc gtgcgcccgc
     3121 cgctgcgcgc ctggacctcg ccgtcggcga tttcgagctg ctccaccgac acgcccaacc
     3181 ggctcgccgc acgctcgagc agcaggtcct tcacttccgc cgccgcgtag cgcagcgcca
     3241 tgccgctttc ctgtatcgag cggctcccgg aggtcatgcc ttcattggga ctgtaggcgg
     3301 tgctcgccgg ggcaagcttg atgcgatcga tcgcgacatc cagctcctcg gcgacgatct
     3361 gcgtaagcgc cgtgaggatg ccctgcccga tttccacctt tccggggtac acggtcacgg
     3421 tgccgtcggg attgatgctg agccagcggc tcagcatgcg gttgccgtcg agactgccgg
     3481 gcagcttgcg cggtacgggt ttgtcagcag gactcattgc gctattcctt tcaccgcgcg
     3541 ccgaatggcg cgcaggatcc gctggtgcgt gccgcagcgg cacagattgt tcgcgagggc
     3601 ggctttgatt tcttcatcac tcggctgcga attcacatcg aggagcgctt tcgccgccat
     3661 gatgatgccc gttgcgcaat atccgcactg ggcggcctgc tcgccgatga aagcctcctg
     3721 taacggatgc aggcgtgcgt cgctggcgat gccttctatt gtggtgatcg gctttccggc
     3781 aaccgcggca accggcacat cgcacgatgg aaccgctttg ccgtccacga tgaccatgca
     3841 cgccccgcaa tgacccgcgc cgcagccgaa gcgcgcgccc ttgagcttaa gttcgttgcg
     3901 cagtacgtag agcagcggcg tttcgggctc cgcgtcgaca acccgcatcg cacgattcac
     3961 gttcagagtg atggccatgg cgagccttgg ttcctaaggt tttaaaaaac agggccttaa
     4021 cagatgactc cggcgctcgc accgcgtgcg gccgggcgcg gctccggtct cgttttttgc
     4081 aaacaatggg actatactga aatcgtgtcc gactgcgaat ggaggactcc gcccatgatg
     4141 atcctggatg tcgaagcgct caaggaacgc atccaccagc tgcacattga gcaccgcgac
     4201 ctcgacgacg taatctcccg actctcacaa agccccgccc aagaccagtt gcaactgcag
     4261 cgactcaaga aacgcaagct ctatctcaag gaccagatcg ctctgctcga gcgtcagttg
     4321 acgcccgaca taccggctta gccgctcgcg gatctcctgt cacgcgggtt ccatgcggag
     4381 gcggcagatt ctagcgcaag ttagagcgcc gcatttcagc gcggcgctcg tgcgcgaacg
     4441 tacagtgcaa gccccgccgc gaaaacgccg agcaggacga accagacgag cgaccagccg
     4501 gctatcgaca gaccgagaaa agtccagccc gcttcggcgc attggcccga tcccctcagc
     4561 actttgtcga gcacctggct caacggaaat tttctgagca tgtattcgag tccgggaccg
     4621 cattcgggca cgcggtcctt gggaaggtgc tggagccaga cgtgacgtgt cgcgatggct
     4681 gcaccgatgc ctgcgatgag cgtcagcagc acgccgtaga tcgcgctgcc gacccgtccc
     4741 ggcccgtgga tcgccgcaac gaggaatacg accccgagtc cgaaaaacgc gacgcgctgc
     4801 agaatgcaca gcggacaggg ttcctggtac tccacgtact ggaggtaata ggcgaacgcg
     4861 atcagcgcga tgcagatcgc agagcccgct ccgtacccga ttctggcggt cgtccgggtg
     4921 tccatcatca taggtaggcg agcagacgtc cgcacgcgat caccccgaac cacaatgcga
     4981 gtgacgtgcc ggcgtggagc cgggccggaa gcggcggggc cacttccgca tcccacagcc
     5041 cgacgccgcg gtacacgccg gcgtggaaca gcgcggcatt gaccccggcc gccagaatca
     5101 gcatgagctt aagctgaaag gcggggttca ccagaaaatc gctcgcatgc gcaatgaaca
     5161 tcagaactcc cgtcggtacg atcacgatga gcgcgccgcc ggtccagggc agcaggtggc
     5221 gcgccatgag gcgcaccgaa agcttgcggg aaagcccgag caaccgcagg tcgaacatcg
     5281 tgatcgagcc caccaggacc acaaagccgg cgatatggag gatctcgacc gcggggtaga
     5341 gccacagctc atgacgcatc gcgagcgcca gcggagaccc ctcaatcgcg gagaacgggg
     5401 tcgtggccgg cgcatcggtc atcgcaattc gacggtcttt ccgtccaccg tgatgcgctc
     5461 ggcgcgcatt tcctcgggct tgctgcggtt cggatagccg gtcaccgtag cccgtgcacc
     5521 cttcttgagc gcgctcgccg gcaaccctcg attggccatg cgcgtcggcg gcgcaagcac
     5581 aacccaccaa gtcttgttgt ccgtgttgag gcggatatga ccgtgcgggt gctcatatcc
     5641 cgactcggca atcgtgcccg agagattgag cggtctcgtg ctgtcgtact cgctccagcc
     5701 gtggtgggcg tgcgcaggca ggcttgcgag gagcaaaacg gacaggcata cggcggatcg
     5761 catcggcagc gcctttcgga ataggccaac gacgatcgta gtttaccgaa attcgctaca
     5821 ctgccgcaca tgatacgtgt cactgctgac atcgtcctcg acgaaagcga gatcgaagag
     5881 cgcttcatac gcgcttcggg cccgggcggg cagaacgtga acaaggttgc aacggcagtt
     5941 cagctgcgct tcaacgtcct gcgctcgaag cgcctcccgg aagcggtgcg ggcccgactc
     6001 atcacgctcg ccggccgccg cctcgccggg gatggctcgc tcgtcattac cgccgagcgc
     6061 ttccgcaatc aacgccgcaa ccgcgacgac gcgctcgacc ggctgattgc gctggtgcgc
     6121 agggcggccg agccgccgaa gcggcggcgt gcgacccggc cgaccgctgc ttcgcgcgag
     6181 cggcgcctcg aaggcaagcg gcgccgcggc gaaataaagc gccgccgggt aagactcgat
     6241 ttgatctgac gaacgcacgc gaagcgcatt gggcgctatg cggcctacgg agcggaatga
     6301 acgtccgggc ccagctgtta tatcatcgcg caccagtcgc gccccatatc gggaaactta
     6361 ggaggaaact acatgcttcg tgcagcagca gtatttcggg ttacatcagc ggcggtcttc
     6421 gcgagtgtca tgtccgtatc caccgtttat tcgcacgcgg cgagcggcga agaagtcatc
     6481 aaggcccgca tcgatttcat gaaggatgag atggaaggaa actggaagcc cctcgccgcc
     6541 ttcgcgaaag gcaaaggatc gctggcggac gtcgagaaac acgcagccgc catttcgaag
     6601 cgcgcgaacg acattccgaa gcactttccg aaggacaccg gacgcggcaa gtatcccgac
     6661 aagctcacgc gcacgctgcc tgcggtatgg gaggactggg atggattcaa gaaggatacg
     6721 cagcggctgg tggaaggaag cgaccggctc gcccgcctcg cgcgcgaagg aaagaaggac
     6781 gaagtgctcg agctgatcgg tccggggggc tatggcagca cgcggatcgg ctgcgcggaa
     6841 tgccacgaca attttcgcgg tccgagagcg aaatagacgc agccataccg atgctcgcgc
     6901 gcctgtgagt gctggccgga agatacgcca gtcgctcacg ggcgcgctgc tctcgattct
     6961 cgcgttgacc tgctctgtac atgcggcggg ggacgcgatg cgcggcgaat acgtcgcccg
     7021 attggcgggc tgccattcct gccacaccga tccccgccgc caggacgc
//
LOCUS       DASURG010000412         3776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1401558, whole genome shotgun sequence.
ACCESSION   DASURG010000412 DASURG010000000
VERSION     DASURG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401558"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFY80_07290"
     CDS             complement(<1..893)
                     /locus_tag="VFY80_07290"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6007750.1"
                     /translation="MQATHTEPIDDLRVREIDELVSPETLLSELPATAAVSATARQAR
                     GAIHRILSQADDRMLAVVGPCSIHDPAAALEYARSLAREAKRLEAELLIVMRVYFEKP
                     RTTVGWKGLINDPALDGTYKVNEGLRLARKLLIDINTLGLPTGQEFLDPITPQYLADL
                     AAWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNVRIAVDAIRAARHPHRFVGVTK
                     AGRSAVVETTGNEDCHLILRGGKEPNYDAKSVQAACSALASAGLPERLMIDCSHANAA
                     KQFRRQLEVAGDVGRQLAEGER"
     gene            complement(957..1565)
                     /locus_tag="VFY80_07295"
     CDS             complement(957..1565)
                     /locus_tag="VFY80_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX6007751.1"
                     /translation="MRMRINPASPFARKVRIVAREANLMSRLEEIDTAVSPVTPNPDL
                     AGANPLVKIPALILDDGTTLYDSRVIAEYLDSLAGAKLFPASGTERWNELRLQALCDG
                     ILDAAVLTRYEMAVRPQPQQWQEWIAGQRRKIDGGVAALEREHAGWGERFRIGQIGAA
                     CVLGYLDFRFPQIDWRSAHPGLKGWFQRVSERTSVRETMPPA"
     gene            1676..2578
                     /locus_tag="VFY80_07300"
     CDS             1676..2578
                     /locus_tag="VFY80_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6007752.1"
                     /translation="MPAEPLIAVFTKNRTNPAYAGARLGADRTAQRLGARTRHFVPDT
                     PDDVEQQIALIDQALAARPDAFVFTPVHENAVDSAIARIDAAGIPLVNFINRMQRGNR
                     IAFVGSDDRALAIAVAERLFVHLNGRGRVAVMQGTPGSITSRTRLEGFLEAAGRHPGV
                     AIVAQCAGDYQRQTARDVMQGVLARHSALDGVLCANDEMALGAIEAMRAAGRMVPVVG
                     VNALPEAIAAIERGTLIATADFDATKMGCIATEAAIRHLRGERIPAEIMLPVQVVDAG
                     NCAAWDRPIEARECPRWEDVAGAA"
     gene            complement(2747..>3776)
                     /locus_tag="VFY80_07305"
     CDS             complement(2747..>3776)
                     /locus_tag="VFY80_07305"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX6007753.1"
                     /translation="VLPKLDVSEIAHIHIKHGNEELNIVHEDGVWRVKERGGFPADPA
                     LISELTFKAREWKIAQSEPISEAQRPRVDVAPPDAKEGASTLLEFRGKDGKPLATLYL
                     GKKHLGKAPLQVKGFDKGQPDGRYLIVGSDPKTLLVVQDPMNNVEPKPEKWIAKEFAK
                     VDRIKTLKVDTGNPQTSYEISREEEAGDWVLAGVRADQTNPSAAVSATNVLYSLSFDD
                     VAPGLKPAQLEKPTLITADTFEGWHYRIQVAPKPGDDEHMYFTIAVTGDVKRTERKPA
                     KDEKPEEKEKRDKEHEERFKRLEAQLAREKTFADWVFVVQRKSLSSLLRERSRFIEEP
                     EKPEPAKK"
BASE COUNT          564 a         1291 c         1275 g          646 t
ORIGIN      
        1 cgctcgccct cggcgagctg ccggccgacg tcgccggcca cctcgagctg gcggcggaac
       61 tgcttcgccg cgttggcgtg cgagcagtcg atcatcaggc gctcgggcag cccggcgctc
      121 gcgagcgcgc tgcacgccgc ctgcacgctc ttcgcgtcgt agttcggctc cttgccgccg
      181 cgcaggatga gatggcagtc ctcgtttccg gtggtttcga ccaccgccga gcggccggcc
      241 ttggtgacgc cgacaaaacg atgcggatgg cgcgcggcgc gtatcgcgtc gacggcgatg
      301 cgcacgttgc cgtcggtccc gttcttgaat cccaccggac acgacagacc cgacgcgagc
      361 tcgcgatgca cctggctctc ggtggtgcgc gcgccgatcg cgccccacgc ggccaggtcg
      421 gcgagatact gcggcgtgat cgggtccagg aactcctggc cagtcggcag gccgagcgtg
      481 ttgatgtcga tcaggagctt gcgcgcaagc cgcaagcctt cgttcacctt gtacgtgcca
      541 tccagcgcgg gatcgttgat gaggcccttc cagcccaccg tggtgcgcgg cttctcgaag
      601 taaacgcgca tcacgatgag aagctccgcc tccaggcgct tcgcctcccg cgcgaggctc
      661 cgcgcgtact cgagcgcggc agccgggtcg tggatggagc acggacccac cacggccagc
      721 atgcgatcgt cggcctgcga taggatacgg tggatcgcgc cgcgggcctg ccgcgcggtg
      781 gccgagactg ccgccgtggc cggcagctcg gagagcaatg tctcgggaga aaccagctcg
      841 tcgatctcgc ggacgcgcag atcgtcgatc ggctcggtat gcgtggcttg cacggtcttc
      901 ctccctacat cagcgcgatg cttccccgga acccgctcgc gccaaaccct cgcgcgctag
      961 gcaggaggca tcgtctcgcg tacggacgtt cgctccgaga cgcgctggaa ccagcccttg
     1021 agcccgggat gtgcgcttcg ccagtcgatc tgcgggaagc ggaagtccag gtagccgagc
     1081 acgcaggcgg cgccgatctg cccgatgcgg aagcgttcgc cccaaccggc gtgctcgcgc
     1141 tccagcgcag ccacgccgcc atcgatcttg cgccgctggc ccgcgatcca ctcctgccac
     1201 tgctgcggct gcgggcgcac cgccatttcg tagcgcgtga gcacggcggc atccaggatg
     1261 ccgtcgcaaa gcgcctgcag ccgcaactcg ttccaccgct ccgtgcccga ggcgggaaag
     1321 agcttggccc cggcgagcga gtcgagatac tccgcgatca cgcgcgagtc gtagagcgtc
     1381 gtgccgtcgt ccaggatcag cgcgggaatc ttgaccagcg gattggcccc cgccaggtcg
     1441 ggattcgggg tgacgggcga gacggccgtg tctatctcct ccagacgcga cataaggttt
     1501 gcttcgcgcg cgacgatgcg caccttgcgg gcaaaggggg aggcggggtt gatgcgcatg
     1561 cgcatggcga tgctcctcgg aaaccgcggc ggtatgcgcg catgctaacc tgagcattcc
     1621 gcgccggcaa tgcgcccggt tcgaggcggg cgggcgctct acaggatgac cgacgatgcc
     1681 cgccgagccc ttgatcgcgg tattcaccaa gaaccggacc aacccggcgt acgcgggcgc
     1741 ccggctcggc gccgaccgga ccgcgcagcg tctcggcgcg cgcacgcgcc atttcgtacc
     1801 cgatacgccg gacgatgttg agcagcagat cgcgttgatc gatcaggcgc tcgccgcgcg
     1861 tccagacgcg ttcgtcttca cgcccgtgca cgagaacgcg gtggacagcg cgatcgcgcg
     1921 catcgacgcg gccgggatcc cgctcgtcaa cttcataaac cgcatgcagc gcggcaaccg
     1981 gattgccttc gtgggctccg acgaccgcgc gctcgcaata gcggtagccg aacggctgtt
     2041 cgtgcacctg aacgggcggg gcagggtcgc ggtcatgcaa ggcacccccg gctcgatcac
     2101 cagccgcaca cggctcgaag gcttcctcga ggccgccggg cgccatccgg gcgtcgcgat
     2161 cgtcgcgcaa tgcgcgggcg actaccagcg ccagaccgca cgcgacgtca tgcaaggggt
     2221 gctcgcccgc cattccgcgc tcgacggggt gctgtgcgcg aacgacgaga tggcgctcgg
     2281 ggcgatagaa gcgatgcgcg cggccgggcg gatggtcccc gtggtcggcg tcaatgcact
     2341 tcccgaagcg attgccgcga tcgagcgcgg gacgctcatc gcgaccgcgg atttcgacgc
     2401 gaccaagatg ggatgcatcg ccaccgaagc ggcgatccgt cacttgcgcg gggaacggat
     2461 accggccgag atcatgctac cggtgcaggt ggtggacgcc ggcaactgtg cggcctggga
     2521 ccggccgatc gaggcgcgcg agtgtccgcg ctgggaagac gtggcaggtg ccgcgtgagc
     2581 gcgacgcggt gcctgtccgg caagtgattt gtcgccgact ctttctgggc gccgcctgtg
     2641 cgggggcagc gtcgccggat gtcattcccg agtaagcgga agcccgggcg cacatgtcct
     2701 cacggtcatg cgtgcatggc gacgaagcgg tgcgacgcga cggaacctac ttcttcgccg
     2761 gctcgggctt ttccggctcc tcgatgaagc ggctccgctc gcggagcagc gacgagagcg
     2821 acttgcgctg aacgacgaac acccagtcgg cgaacgtctt ctcgcgcgcc agctgcgctt
     2881 ccagacgctt gaagcgctcc tcgtgctcct tgtcgcgctt ttccttctcc tccggcttct
     2941 cgtccttcgc cggcttgcgc tcggtgcgtt tcacgtcgcc ggtgaccgcg atcgtgaagt
     3001 acatgtgctc gtcgtcgccg ggcttcggcg cgacctggat gcggtagtgc cagccctcga
     3061 acgtatccgc ggtgatcagc gtcggctttt ccagttgcgc cggtttcagc ccgggcgcaa
     3121 cgtcgtcgaa cgagagactg tagagcacgt tcgtcgcgct caccgcagcc gacgggttgg
     3181 tctggtcggc gcgcacgccg gcgagcaccc aatcgcccgc ctcttcctcg cgcgagatct
     3241 cgtagctcgt ctgcggattg cccgtatcga ccttcagcgt tttgatgcgg tcgaccttcg
     3301 cgaattcctt cgcgatccat ttctcaggct tgggctcgac gttgttcatc gggtcctgca
     3361 cgacgagcaa tgtcttcgga tcggaaccga cgatgaggta gcgcccgtcg ggctggccct
     3421 tgtcgaagcc cttcacctgc agcggcgcct tccccaggtg cttcttcccg aggtagagcg
     3481 tggcgagcgg cttgccatcc ttgccgcgaa actcgagcag cgtggaggcg ccttccttgg
     3541 cgtcgggcgg cgccacgtcg acccgggggc gctgcgcctc ggagatcggc tcgctctgcg
     3601 caatcttcca ctcgcgcgcc ttgaacgtga gctcggagat gagcgccgga tcggcgggaa
     3661 agccgccgcg ctccttcacg cgccacacgc cgtcttcgtg gacgatgttg agctcctcgt
     3721 tcccatgctt gatgtgaata tgggcgatct cgctcacgtc gagcttgggc agcacg
//
LOCUS       DASURG010000413         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_992351, whole genome shotgun sequence.
ACCESSION   DASURG010000413 DASURG010000000
VERSION     DASURG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992351"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFY80_07310"
     CDS             <1..813
                     /locus_tag="VFY80_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6007754.1"
                     /translation="AGYAAPPRTWSEWHEMLAAITRAGQDERYGILLPLNEPEPLIAL
                     AMQQPEPLLREEDRWSGFSSTGFRRALAFYASMFENGWAPRVGGAQISNVWAEFGRGR
                     FAFYVSGPWNIGEFKRRLPPEVQAHWMTAPLPGPDGPGASTAGGSSLVIFRSAQAREA
                     AWRLVEFLSEPAVQLRFHRLTGNLPPRRSAWQDQELASDPHASAFREQLERVKAAPKV
                     PEWERIVSEVQYMAEEVVRGRASIEQATRTLDSKVNAILEKRRWLLARERTQ"
     gene            810..1682
                     /locus_tag="VFY80_07315"
     CDS             810..1682
                     /locus_tag="VFY80_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6007755.1"
                     /translation="MTHPAAWAFIAPALIVIGVFFLVPVAAGFALSLTDFDIYALADF
                     GNLRFVGLDNYVRMLETPLFWQALGNTLYFVIVGVPLSIGLSLAAALLLHSRLARFRP
                     LFRTALFAPVVTTIVAVAVIWRYLFHTRYGVVNHALGSVGIGPIDWLGDPAWAMPTII
                     LLAVWKNFGYNMVIFLASLQAIPETLYEAARIDGASWWRQIVHITLPMLGPTLLLVAI
                     LTTAGYFQLFAEPYVMTQGGPLRSTVSVLYFMYEEGFKWWNFGAASAVALMLFGLVFA
                     VTWFLMRISRRVAA"
     gene            1679..2500
                     /locus_tag="VFY80_07320"
     CDS             1679..2500
                     /locus_tag="VFY80_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6007756.1"
                     /translation="MNPRLAAITVNGLLVGLTVVALFPLLWMLSVSFMPTGAASALPP
                     PLFPSAPTLAHYRELFTSGGMLRWLGNSLLVALAITALSLAFNTMAGYAFAKLKFHGR
                     ERLFNSLIGALIIPGQVAMMPLFLLLKEMGLVNSYGGVIVPAMASVFGIFLVRQYART
                     IPDELIEAARVDGASEFRIFVSIVVPLLKPVLASLAVFTFLAAWNDFMWPLIVLTDNT
                     LQTLPVALATLSREHVQDSELMMAGSVVTVLPVLVLFLALQRYYIEGLVLGSVKG"
     gene            2500..>4879
                     /locus_tag="VFY80_07325"
     CDS             2500..>4879
                     /locus_tag="VFY80_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX6007757.1"
                     /translation="MSKTAGCTLAARTRSACLCILGFLLLATSSSSHAAPMDMTLRVL
                     DDFDDPTQWQASASDDVKARLFRATGRAGGALCLEFDFGTVSGYAVARRRLPIEFTGN
                     YEMTLALKGDAPANMLQLKLVDASGENVWWVNRPDFDFSRDWETLRFKRRHIGFAWGP
                     TQDRTLKRSAFIELAIVRGRGGGKGTVCLDELTLRALPSAAAPMAPRVTATSSFQSSV
                     PAHVIDGNMDTVWRSDPAAAEEQALTLDFGASREFGGLVLQWAPGTHATRYAIEYSDD
                     GERWRTVRQVAAGNGGKDPHLLSESETRYLRLRMQKGNGHGYGLAEVEVKDVEWGASP
                     NAFFEALAKAAPRGHYPRAYRAEQTYWTVLGSDGGERHGLLSEDGAVEPAPRSPSVEP
                     LLLLDGTVVTWADVSIEHELRDGYLPLPRVTWRGSDWALHVTAFAAATDRHGTLTARY
                     AFENRTDRIRRVTLALAIRPFQVNPPSQFLNVPGGVAPIRELKWDGRAIAVNGQVMVY
                     PLEPPQSVMLAPFDAGNVLELLAGGAFRESSALSDETGFASGVLLYRMDIPPREQRTI
                     GVVLPRAGVPDRLEPARGWLERAEDETAETWREKLNRVALHLPPDAQHIAHTVRTALA
                     HVLASRAGAALQPGTRAYARSWIRDGAMMSDALSRMGHAAVARDYAEWFAPYQFSNGK
                     VPCCVDDRGADPVAENDSHGAFIHLIAQHYRYSADQDWLRRMWPRVDAAARYMNGLRL
                     SERTARNQDPERRAYYGLMPPSISHEGYSDKPAYSYWDDFWTLAGYDGALEIA"
BASE COUNT          771 a         1554 c         1613 g          941 t
ORIGIN      
        1 gccggatacg cggcaccgcc acggacatgg tccgagtggc acgagatgct cgcggcgatc
       61 acccgcgcag gacaggacga gcgctacggc atcctcctcc cgctcaacga accggagccg
      121 ctgatcgcgc tcgcgatgca gcaacccgag ccgctcttgc gcgaagagga ccgttggagc
      181 ggcttttcca gcaccggttt tcgccgcgcg ctcgcgtttt acgcctcgat gttcgaaaac
      241 ggctgggcgc cgcgcgtcgg aggcgcgcag atctcaaacg tctgggcgga gttcgggcga
      301 gggcggtttg cattctatgt ctcgggcccc tggaacatcg gcgaattcaa gcggcgactt
      361 ccgccggagg tgcaggctca ctggatgaca gcgccgctgc cgggacccga cgggccgggc
      421 gcctccacgg ccgggggatc gagcctcgtc atctttcgca gcgcccaagc gcgtgaagcg
      481 gcctggcggc tggtcgagtt tctctccgag ccggcggttc agcttcgctt tcaccgcctc
      541 accgggaacc tgcctccgcg gcgcagcgca tggcaggacc aggagcttgc atccgatccc
      601 cacgcgagcg cgttccgcga acaactggag cgcgtaaagg cggcacccaa ggtgcccgag
      661 tgggaacgca tcgtgagcga agtgcagtac atggccgagg aagtcgtacg cggtcgcgca
      721 agcatcgaac aggcgacgcg cacgctcgac agcaaggtga acgcgatcct ggagaagcga
      781 cgctggctgc tcgcgcgcga aaggacgcaa tgacgcatcc cgcagcatgg gcgttcattg
      841 cgccggcgct catcgtgatc ggcgtctttt ttctcgtgcc tgttgcagcg ggattcgcgc
      901 tcagcctgac cgacttcgac atctatgcgc tggctgactt cgggaacttg cgcttcgtcg
      961 gcctcgacaa ctacgtgcgg atgctggaga cgccgctttt ctggcaggcg cttgggaata
     1021 cgttgtactt cgtcatcgtc ggcgtaccgc tttcgatcgg gctttcgctt gccgctgcgt
     1081 tgctcctgca ttcgcggctc gcgcgtttca ggccgctttt ccgcaccgcg ctctttgcgc
     1141 cggtcgtgac gaccatcgtg gcggtcgcgg tgatctggcg ctacctcttc cacacccgtt
     1201 acggggtcgt aaatcacgcg ctgggaagtg tcggcattgg gcccatcgac tggcttgggg
     1261 atccggcatg ggcgatgccg accatcatcc tgctcgccgt gtggaagaac ttcggctaca
     1321 acatggtgat cttccttgcg agcctccagg cgattcccga gacgctctat gaagccgcgc
     1381 gcatcgacgg cgcctcttgg tggcggcaga tcgtgcacat cacgttgccg atgctcgggc
     1441 cgacgctgct cttggtcgcc atcctcacga ccgccggata cttccagctt tttgccgagc
     1501 cctacgtgat gacgcagggc gggccgttgc ggagcaccgt gagcgtcctc tacttcatgt
     1561 acgaggaagg cttcaaatgg tggaatttcg gcgcagcgtc cgcggttgcc ctgatgctgt
     1621 tcgggctcgt gtttgctgtg acctggttcc tcatgcgcat ttcgcggcgg gttgcggcat
     1681 gaaccctcgc ctggcagcaa tcaccgtcaa cggcttgctc gtcgggctga cggtcgtggc
     1741 gctctttccg ctgctgtgga tgctctcggt ctcgtttatg ccgacgggcg ccgcgagcgc
     1801 cctgccgccg ccgctttttc catcggcgcc gacccttgcg cactatcgtg agcttttcac
     1861 gagcggcggg atgctccgtt ggctcgggaa cagccttctt gtcgcgcttg ccatcaccgc
     1921 cctctcgctt gcattcaaca cgatggcggg ctatgccttc gccaagctca agtttcacgg
     1981 gcgcgagcgg cttttcaaca gcctgatcgg tgcgctgatc attcccgggc aagtcgccat
     2041 gatgccgctc ttcctgctgc tcaaggaaat ggggcttgtg aattcctacg gcggcgtgat
     2101 cgtgccggcg atggcgagcg tattcgggat cttcctcgtg cgccagtatg cgcgtaccat
     2161 acccgacgaa ctgatcgaag ccgcgcgcgt cgatggtgcg agtgaattcc gcatctttgt
     2221 ctcgatcgtc gtgccgctgc tgaaacccgt gctcgcctcg ctggcggtct tcacctttct
     2281 cgctgcatgg aacgacttca tgtggccgct catcgtgctg accgacaaca cgctgcagac
     2341 gctgccggtt gcgctcgcga cgctctcccg cgagcatgtg caggacagcg agctcatgat
     2401 ggccggctcc gtcgtgaccg ttcttccggt gctcgtgctc tttctcgcgc tgcagaggta
     2461 ctacatcgaa gggctcgtgc tcgggagcgt gaagggttga tgagcaagac agcggggtgc
     2521 acgctcgccg cgcgcacccg tagcgcgtgc ctgtgcattc ttggcttcct gctgctcgcc
     2581 acgtcttctt cttcccacgc cgcacccatg gacatgacgc tccgcgtgct cgacgatttc
     2641 gatgatccga cgcagtggca ggcatcggct tcggatgacg tgaaggcgcg gcttttccgt
     2701 gcgacaggcc gtgcgggcgg tgcgttgtgt cttgaattcg atttcgggac ggtgtccggt
     2761 tatgccgtcg cgcgtcgcag gcttcccatc gaattcaccg gaaactacga gatgacgctg
     2821 gcactgaagg gcgatgcgcc ggcgaacatg ctgcagctga agctcgtcga tgcgagcggc
     2881 gagaacgtgt ggtgggtcaa ccgcccggac ttcgattttt cgcgcgactg ggagacgctg
     2941 cgcttcaagc gccggcacat cggtttcgca tgggggccga cgcaggaccg cacactcaag
     3001 cgtagcgcgt tcatcgagct ggccatcgta agagggcgcg gcggcggcaa aggcaccgta
     3061 tgtcttgatg aactcacgct gagagcgttg ccttccgcgg ccgcgccgat ggcgccgcgc
     3121 gttacggcta cttccagttt tcagtcgtca gtccccgctc acgtcatcga cggcaacatg
     3181 gacacggtgt ggcgaagtga tcccgcggcg gcggaagagc aggctctgac gctcgatttc
     3241 ggtgcgtcac gcgaattcgg cggcctcgtc ctgcagtggg cgccgggaac gcacgcgacg
     3301 cgctatgcga tcgagtactc ggacgatggc gaacgctggc gcacggtgcg gcaggttgcc
     3361 gccggcaacg gtgggaagga cccgcatctg ctctccgagt cggagacgcg ctatctcagg
     3421 ctccgcatgc agaaagggaa cgggcacggt tacgggctgg cggaagtaga ggtgaaggac
     3481 gtggagtggg gcgcgtcacc caacgcattc ttcgaggcgc ttgccaaagc ggccccccgt
     3541 ggccattacc cgcgcgctta ccgggctgag cagacatatt ggaccgtgct cgggagcgac
     3601 ggcggggagc ggcacgggct cctctcggag gacggcgcag tcgagcccgc gccgcgttcc
     3661 ccttcggtcg agccgttgct gctgctggac ggcacggtgg tcacgtgggc ggacgtgagc
     3721 atcgagcacg aattgcggga cggctatctg ccgctgccac gcgtgacgtg gcgcgggagc
     3781 gattgggcct tgcacgtcac ggccttcgct gcggcgacgg atcggcatgg gacgctcacc
     3841 gcccgatatg ccttcgagaa ccgaaccgat cggatccggc gcgtgacgct cgcactcgcg
     3901 atccgcccgt ttcaggtgaa tccgccgtcg caattcctca atgtgccggg cggcgtcgcg
     3961 ccgatccgcg aactcaagtg ggatggaaga gcgattgcgg tgaacggcca ggtgatggtg
     4021 tatccgctcg agcctccgca gagcgtgatg ctggcgccgt tcgatgcagg aaacgttctc
     4081 gagctcctcg ccggcggtgc gttccgggaa agcagcgccc tgagcgacga gaccggattc
     4141 gcttccggcg tcctgctcta tcgcatggac atcccgccgc gcgaacagcg cactatcgga
     4201 gtcgtcctgc cccgggccgg cgtaccggat cggctcgagc ccgcacgggg ctggcttgag
     4261 cgtgcggagg acgaaactgc agaaacgtgg cgcgagaagc tgaaccgcgt tgcgctgcac
     4321 ctgccacccg atgcgcagca tatcgcgcat accgtgcgca cggcgctcgc gcacgtactt
     4381 gcgagccgcg ccggggccgc actgcagccc gggacccggg cttacgcgcg ctcgtggata
     4441 cgggatggcg cgatgatgtc cgatgcactc tcccggatgg gccacgccgc ggtggcgcgc
     4501 gattatgcgg aatggttcgc gccgtatcag ttcagcaatg gcaaggtgcc gtgctgcgtg
     4561 gatgatcggg gtgcggaccc cgtggccgag aacgacagcc acggcgcctt catccatttg
     4621 atcgcgcagc actaccgcta cagcgctgac caggattggc tgcgccgaat gtggccccgc
     4681 gtggacgcgg cggcgcgtta catgaacggt ttgcggcttt ccgagcgcac ggcaagaaat
     4741 caggaccctg agcgccgtgc ctattatggc ctcatgccgc cttcgatcag ccacgagggc
     4801 tactccgaca agccggcata ctcctactgg gacgacttct ggacgctagc cgggtacgac
     4861 ggtgcgcttg agatcgcgc
//
LOCUS       DASURG010000414         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1298771, whole genome shotgun sequence.
ACCESSION   DASURG010000414 DASURG010000000
VERSION     DASURG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298771"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="VFY80_07330"
     CDS             <1..1809
                     /locus_tag="VFY80_07330"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX6007758.1"
                     /translation="QKNHYLPRLAQGIDIPCFALTGPEAGSDASSIPDTGIVCRASFE
                     GRSDVLGIRLNWEKRYITLGPIATLIGLAFRLRDPDHLLGDTDDIGITLALIPRTTPG
                     VSIGRRHFPLNSAFFNGPNYGKDVFLPMESVIGGREYVGQGWRMLMNCLAAGRSISLP
                     ANSVGIAKLAALTTGAYGRVRIQFRTPIGRFEGVEEALARIGGHTYMMDAARIMTAGA
                     IDCGEKPAVVSAIVKYHLTERGRMVINDAMDVHGGKAICMGPRNYLGRSYQQLPIAIT
                     VEGANILTRSMIIFGQGALRGHPYVLREIAAAGERDPAKALRLFDEAFFGHVKFILAN
                     AARALFLGLTAARFTRVPGDAHTQPYYRQLARWSAAFAFATDVAMLRLGGQLKRKEKL
                     SARLGDVLSQLYLCSAALKRHEDGGRPHDELPLLHWSVQDALFRLQAAFAGLIDNFPG
                     SIARALLRVTAFPAGRRFAPPSDALGHEVAALLLEPSKVRERLTRGVYIPKSGDEPIA
                     QLEHALRAAIAVESIYSRIYAAARAGRISGSTADELALQAESAGIIDPAELNALDLAK
                     ALRRAVIAVDDFPPDLGRDTEAYAAATPVGAARKMA"
     gene            1898..>3109
                     /locus_tag="VFY80_07335"
     CDS             1898..>3109
                     /locus_tag="VFY80_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6007759.1"
                     /translation="MAGPGAHVIPAVSARTLDGLFRERVDRTPDAVACRHFNETHGVW
                     RDYNWGQLNHQVARWQSALLRDGLKSGDRVAVMLRNSPEWVVFDQAALGLGLVVVPLY
                     TQDRPENVAYITRDAGCKVLLFEGLGQWQALRDVREQLSGLVRILAVNPQVEDGTEPR
                     LRSIAEWLPEQGGEVQHVPSDPHGLATIVYTSGTTGRPKGVMLSHHNILSNAEACLSV
                     LTPRPDDVFLSFLPLSHTFERTCGYYLAVMTGAATAYARSIPQLAEDLQSIRPTLLIS
                     VPRIYERVYGAIRSKLAEGPAFRRRLFELALDTGYARFEHEQGRTPWQPSFLLWPLLD
                     ALVAAKIRARLGGRLRAAISGGAALAPEISRVFIGLGITVLQGYGLTETSPVACANRP
                     DDNLPASVGRAM"
BASE COUNT          536 a          981 c         1008 g          584 t
ORIGIN      
        1 cagaaaaacc attatctgcc ccggctcgcg caaggaatcg acatcccgtg ctttgcactg
       61 accggtccgg aggcggggtc ggatgcgagc tcgattccgg ataccggcat cgtgtgtcgc
      121 gcgtcgttcg aaggcagatc cgatgtgctg gggatccgcc tcaactggga gaaacgctac
      181 attacgctgg gccccatcgc gacacttatc ggtctcgctt tccggctgcg cgacccggat
      241 caccttctgg gcgataccga tgatatcggc atcacgctcg ccctgatacc gcgaacaacg
      301 cccggtgtca gcatcgggcg gcgccacttt ccgctcaatt ccgctttttt caatgggccg
      361 aattacggca aggacgtatt cctgccgatg gagtctgtga tcggcggccg ggaatacgtc
      421 ggccagggtt ggcgcatgct gatgaactgc cttgcagccg gccgttcgat ttcgcttccc
      481 gcgaacagcg tgggaatcgc caagctcgcc gcattgacga ccggtgccta cggccgtgta
      541 cgcatccagt tccgcacgcc gatcggccgt ttcgagggcg tggaagaagc gctcgcgcga
      601 atcggcggac atacctacat gatggacgca gcgcgcatca tgaccgcggg tgccatcgac
      661 tgcggcgaaa aacccgctgt cgtttcggcg atcgtgaaat atcacctgac cgagcgcgga
      721 cggatggtga tcaacgacgc aatggatgtt cacggtggca aggcgatctg catggggccg
      781 cgcaactatc tcggccgctc ttaccagcaa ctgcccatcg caatcacggt cgagggcgcg
      841 aacatcctca cgcgctccat gatcattttt ggccaaggag cgctacgggg tcacccctac
      901 gttctgcgcg aaatcgcggc ggcgggcgag cgcgatcccg cgaaggcgct gcggctgttc
      961 gacgaggcct ttttcgggca cgtaaaattc attcttgcga atgcggcgcg cgcgcttttt
     1021 ctcggtctta cggcggcccg cttcacacgc gttccgggcg acgcgcatac gcaaccttat
     1081 taccgccagc tcgcgcggtg gagcgccgct ttcgccttcg ccaccgatgt ggccatgctg
     1141 cgcctgggcg gccagttgaa gcgcaaggag aagctttcgg cgcgactggg tgacgtcctg
     1201 agccagcttt acctgtgttc cgccgcactc aagcgccacg aggatggcgg aaggccgcat
     1261 gacgagctgc cgctcctcca ctggtcggtg caggatgcgc tctttcgcct tcaggctgcg
     1321 tttgcagggc tcatcgacaa ctttccgggc tcgattgcgc gggcgctgct gagggtaacg
     1381 gcatttccgg ccggccgccg attcgcgcca ccgagcgacg cgctcgggca cgaggtggcc
     1441 gcattgctgc ttgaaccctc taaagtgcgg gagcggctga cgcgaggcgt ttacatcccc
     1501 aagagcggtg atgagccgat tgcgcagctc gagcatgcat tgcgggctgc cattgcagtt
     1561 gaaagcatct acagccgcat ctatgctgcc gcgcgagcgg gacggatcag cggcagcacc
     1621 gccgatgagc ttgcgctgca ggccgagtcg gctggcatca tcgaccccgc tgaactgaac
     1681 gcgcttgacc ttgccaaagc gctgcgccgc gcagtcatcg cggtggacga ttttccacct
     1741 gacttgggac gggacacgga ggcgtacgcg gcagcaacac cggtcggtgc agcgcgcaag
     1801 atggcttaag atgtcgtccg ctggcggcgc acgaaatcca ttttcgtcgc tgggtccacg
     1861 gcgaactcaa ttcaggcaag gacgggagga gcaaagcatg gcaggacccg gggcacatgt
     1921 cattccagcc gtgtcggcgc gcacgctgga tgggctcttt cgcgagcgcg tggatcgaac
     1981 gcccgacgcc gttgcctgcc ggcatttcaa cgagacgcat ggcgtgtggc gagattacaa
     2041 ttggggccag ctcaatcacc aggtcgcgcg ctggcagtct gccttgctgc gggacgggct
     2101 gaagagcggc gatcgggtcg cggtgatgct gcgcaattcg ccggagtggg tggtcttcga
     2161 tcaggccgcg ctcggactgg ggcttgtcgt cgtaccgctt tacacgcagg accgcccgga
     2221 aaacgtcgcg tacatcactc gggatgcagg ctgcaaagtg ctgctcttcg aaggattggg
     2281 gcaatggcag gcgctgcgcg atgtgcgcga gcagctttcc gggctcgtgc gcatactcgc
     2341 cgtcaacccg caggtggagg acggtaccga gccacgactg aggtcaatcg ccgaatggtt
     2401 gccggagcag ggcggcgagg tgcagcatgt cccgagcgat ccgcatgggc tcgcgaccat
     2461 cgtctacacc tcgggcacga cgggtcggcc gaagggcgtc atgttgtcgc accacaatat
     2521 tctgagcaac gccgaagcgt gcctttcggt gctgaccccc cggccggacg acgtcttctt
     2581 gtcgttcctc ccgctctcgc atacgttcga gcgcacctgc ggctattatc tcgccgtcat
     2641 gacaggtgcg gcgaccgcgt atgcccgctc cataccgcag cttgcggagg atctccagag
     2701 catccggccc acgctcctca tctcggtccc gcgcatatac gagcgggtgt acggggcgat
     2761 ccggagcaag ctcgcggaag gtccggcatt cagacggcgg cttttcgagc ttgcgctcga
     2821 taccggctat gcgcgattcg agcacgagca gggacgcacg ccgtggcagc cctcattcct
     2881 gctgtggccg ctgctcgatg cgctcgtcgc cgcgaaaatt cgtgcccgcc tcggtggacg
     2941 cttgcgcgcc gcaatatcgg gcggcgccgc gcttgcgccg gagatttcgc gcgtcttcat
     3001 cggccttggc atcaccgtgc tgcagggcta cggactgacg gaaacgagtc ccgtggcgtg
     3061 cgccaatcgg ccggatgaca atctgccggc gagcgtcgga cgcgccatg
//
LOCUS       DASURG010000415         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1196914, whole genome shotgun sequence.
ACCESSION   DASURG010000415 DASURG010000000
VERSION     DASURG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196914"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /gene="dxs"
                     /locus_tag="VFY80_07340"
     CDS             <1..1266
                     /gene="dxs"
                     /locus_tag="VFY80_07340"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX6007760.1"
                     /translation="LLSGRVYARAKGLGERVLGELARRAEEHVKGMVTPGTLFEEFGF
                     NYIGPIDGHDLDALIPTLNNIKHLSGPQFLHVVTRKGQGYKLAEADPVLYHGVSKFDR
                     VNGISGGKSGGKQTYTQVFGEWVCDMAARDARLIAITPAMREGSGLVKYAELYPERYF
                     DVGIAEQHALTFAAGLACEGYKPVVAIYSTFLQRAYDQLIHDIQIQNLPIVFAIDRAG
                     LVGADGATHHGSFDIAYLRCLPNMTVMTPADENECRQMLYTAFQMNSPAAVRYPRGTG
                     PGVPISVEMRALPIGKGEIRREGAAVGSRVAILAFGAMLTPALEAAEAFDATVANMRF
                     VKPIDEALILELADRHALLVTVEENAVMGGAGSAVAETLQAHRRVVPMLQLGLPDRFI
                     DQGDPGIQLAECGLNRDGIVKAIRARLGE"
     gene            1272..1934
                     /gene="folE"
                     /locus_tag="VFY80_07345"
     CDS             1272..1934
                     /gene="folE"
                     /locus_tag="VFY80_07345"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX6007761.1"
                     /translation="MDDRSNPAAMTLTKEMIEQNEIDGYAKIDQYNPVIIRRIAEHYR
                     SILRDIGENPDREGLLKTPERIAKALQFLTHGYDLDPAGILRSAMFREEYQQMVIVKD
                     IEIYSLCEHHLLPFFGKAHVAYIPNGYIVGLSKIPRVVDAFARRLQVQERLTVDIRNC
                     IDETLKPLGVAVVIEAQHLCMIMRGIQKQHSVATTSAFTGEFNAEKTRAEFMRLITRA
                     GG"
     gene            1951..>2267
                     /locus_tag="VFY80_07350"
     CDS             1951..>2267
                     /locus_tag="VFY80_07350"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX6007762.1"
                     /translation="MVSRTSEYIVERVLPDVRRGRMVILVDSEDRENEGDLFLAAERA
                     TPEAINFMAQHGRGLISLALTEERMRALGLALIADDQINTTKFQTAFATPIDAREGVG
                     SGMS"
BASE COUNT          437 a          704 c          741 g          385 t
ORIGIN      
        1 ctcctgtcgg gccgcgtcta cgcgcgcgcg aaaggactgg gcgagcgcgt cctcggggaa
       61 ctcgcgcggc gcgccgaaga gcatgttaaa ggcatggtga cgccgggcac gctgttcgag
      121 gagtttggct tcaattacat cggcccgatc gacggccacg atctcgatgc gctcatcccg
      181 acactcaaca acatcaagca cctgagcggt ccgcagttcc tgcatgtcgt cacacgcaaa
      241 ggacagggct acaagctcgc ggaagcggat cccgtgctct atcacggcgt ctccaagttc
      301 gaccgcgtga acggcatctc gggcgggaag tcgggcggca agcagacgta cacgcaggtg
      361 ttcggagaat gggtgtgcga catggcggcg cgtgatgcgc ggttgattgc gatcaccccg
      421 gcgatgcgcg aaggatcggg gctcgtgaag tacgccgagc tgtatcccga gcgctacttt
      481 gacgttggca tcgccgagca gcacgcgctc accttcgctg cgggcctcgc ctgcgaaggc
      541 tacaagccgg tcgtggcgat ctattccacc ttcctgcagc gcgcctacga tcagctgata
      601 cacgatatcc agatccagaa tctgccgatc gtgtttgcga tcgaccgcgc gggcctcgtg
      661 ggcgcggacg gtgcgaccca tcacggcagc ttcgatatcg cgtatctgcg ctgcctgccg
      721 aacatgaccg tgatgacgcc ggccgacgaa aacgaatgcc ggcagatgct ctacacggcg
      781 tttcagatga attcgcccgc cgcagtgcgc tatccgcgcg gcaccggacc cggcgttccg
      841 atcagcgtgg agatgcgcgc cctgccgatc ggcaagggtg aaattcggcg tgagggcgct
      901 gccgtcggga gccgggttgc gatcctggcg ttcggcgcca tgctcacgcc cgcgctcgag
      961 gcggcggaag cattcgatgc cacggtggcc aacatgcgtt tcgtgaaacc gatcgacgaa
     1021 gcgctgattc tcgaacttgc ggaccggcac gcgctgctcg tgacggtaga ggagaatgcc
     1081 gtgatgggcg gcgccggaag cgccgtggca gagacattgc aggcgcatcg ccgcgtggtg
     1141 ccgatgctgc agctcggact tcccgaccgc ttcatcgacc agggcgaccc gggaatccag
     1201 cttgctgaat gcggtctcaa cagggacgga atcgtgaagg cgattcgggc gcgactcggg
     1261 gagtagaata aatggacgat cgttcgaatc cggcagccat gacgctcacc aaggaaatga
     1321 tcgaacagaa cgagatcgat ggctacgcca agatcgatca gtacaaccct gtcattatcc
     1381 ggcgcatcgc cgagcactac cgctcgatct tgagggacat cggcgagaat ccggaccgcg
     1441 aggggcttct gaagacgccg gagcggatcg cgaaagcgct gcagttcctc acccacggct
     1501 acgatctcga cccggccggc atcctgcgct cggcgatgtt ccgcgaggaa taccagcaga
     1561 tggtgatcgt gaaggatatc gaaatctact cgctgtgcga gcatcatctg ctgccgttct
     1621 tcggcaaggc gcacgtcgcc tacattccga acggctatat cgtgggcctc tccaagatac
     1681 cgcgcgtcgt ggacgcgttc gcgcggcggc tgcaggtgca ggagcggctt acggtcgata
     1741 tccgcaactg catcgacgag acgctgaagc cgctcggcgt ggccgtcgtg atcgaggcgc
     1801 agcacctgtg catgatcatg cgcggcatcc agaagcagca ctcggtggcg accacatcgg
     1861 cgttcaccgg ggagttcaac gctgagaaga cgcgcgcgga attcatgcgg ctcatcacgc
     1921 gcgccggagg ctgacgttcg cgtctgcacg atggtgagcc gcaccagcga atacatcgtc
     1981 gagcgcgtcc tgcccgacgt ccgccggggg cgcatggtga tcctcgtgga cagcgaagat
     2041 cgggagaacg agggcgatct cttcctcgcg gcggaacgcg cgactccgga agcgatcaac
     2101 ttcatggcgc agcacgggcg cggtctcatc tcgcttgcgc tcaccgagga gcggatgcgc
     2161 gcgctcggcc tcgcgctcat cgccgacgat cagatcaaca ccaccaaatt ccagacggcg
     2221 ttcgcgacgc cgatcgacgc gcgagagggc gtgggctccg gcatgtc
//
LOCUS       DASURG010000416         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_992812, whole genome shotgun sequence.
ACCESSION   DASURG010000416 DASURG010000000
VERSION     DASURG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992812"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFY80_07355"
     CDS             complement(<1..211)
                     /locus_tag="VFY80_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEX6007763.1"
                     /translation="MATASPTLASVEMIRRLVGFPTVSRDSNLELIDFLRECLRPHDA
                     RVRMTYDDGRRKANLFATLGPDDAGG"
     gene            complement(329..1288)
                     /locus_tag="VFY80_07360"
     CDS             complement(329..1288)
                     /locus_tag="VFY80_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007764.1"
                     /translation="MRAFCGLALSLLLAGAALAQEYPTRPIRVLVPFAPGGVVDTSTR
                     ILTTKLTERLGWNFVVDNRPGGNGFIAVQIAAKGAPDGYTLLSAHTGEFAVNPAIFRN
                     VPYDLDRDFAPITMLSDAPMAVVVGANSPINGFKDLVALARAKPGQVTFGSPGTGSIN
                     HLATEWLASAAGVKLLHVPYKGGAPAVSATAAGEVMMTVAGIPGVLPHLQAGRVKVIG
                     ITTAKRSAHANYPTPQEGGISGVDASIWVGLFAPKGTPKNIVNRLYKETVEVLKLADV
                     KERFGTVGGADTMGMPPAEFHARVKRDLERYRQVVQAVGIEPQ"
     gene            complement(1300..2751)
                     /locus_tag="VFY80_07365"
     CDS             complement(1300..2751)
                     /locus_tag="VFY80_07365"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6007765.1"
                     /translation="MSAVAGNPLDLRVWLDEARRLGELKEVRGAHWNLELGAISEVNV
                     RKALPPALLFDEIVGYPKGFRVLTCSTASPARLSSILRLGVETSHHALVRKLRGKPKS
                     WQAEAARYDPVETETGPVFENVQDNGGVNLEAFPSPLWHEKDGGRYIGTGCCVVTRDY
                     DSAWVNVGTYRVQLHDRNHVGLDMVPGKHGAIQYDKYMKARKPFPVAIVLGCDPLGYL
                     ISGIEVPFGMCEYNYIGAILGEPVSVAKGPVTGLAFPAASEIVLEGWAHPGAQRTEGP
                     FGEFHGYYPGKAHAAPVVTIERVYYRNDPILLGSPPAKPPNDYSYSKAVMRSALLFDA
                     LIAAGVPDVKAVWAHEIGGARMFNVVAINQRYAGHAKQAGHILSQCGVGAYMSRYSVV
                     VDEDIDPSNLQEVMWAVATRTDPEVDIDIIRRGMGSKNDPMFVAYRYAAPYSSKAVID
                     ACRPYDHLDDFPDVAEASRELQDEVRAKWKDIL"
     gene            complement(2748..3221)
                     /locus_tag="VFY80_07370"
     CDS             complement(2748..3221)
                     /locus_tag="VFY80_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6007766.1"
                     /translation="MTRTIQFTLNGECVSAEIEPHHNLVDMLQRQFALHGARESCGQG
                     LCGCCTVLVNGKAVSGCLYLAALVDGAEVTTIEYDAGHGLDAVQQAYIECSAFQCGFC
                     TPGFVLMTRQLLDAHPDPSDEEIRHYLSGNLCRCATYPEVIDAVKLAARKRKRAA"
     gene            complement(3218..>3975)
                     /locus_tag="VFY80_07375"
     CDS             complement(3218..>3975)
                     /locus_tag="VFY80_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6007767.1"
                     /translation="VVHPDTDVTPYDMATLGSRSTYHMGHAVRLAAGEARDKLLALAR
                     ELELSAETTPIPELFRKKYGMQAGKIVGVGSFIPSYTPTDHETGLSDNVTPFWMVGGT
                     GVEVEVDTETGHVTVTRLVNVVDAGRPVNPRIVETQLSGAALMQLGFTLSEKMSFDAG
                     QVTNASLAEYKIPGMRDIPLVIENQAVDAAQASGPFGAKGVGESGTFGVSPAIANALH
                     DAVGVRLTSLPITPEAVYRAIRRANGQPLEDDR"
BASE COUNT          693 a         1324 c         1244 g          714 t
ORIGIN      
        1 tgccgcccgc atcatcggga ccgagcgtcg cgaacagatt cgccttacgg cggccgtcgt
       61 cgtaagtcat gcgcacgcgc gcgtcatgcg ggcgcaggca ttcgcgcagg aaatcgatga
      121 gctcgagatt ggagtcgcgg ctgacggtag ggaagccgac gagcctgcga atcatttcga
      181 cgctcgccag cgtcggactc gctgtagcca tgatgcaccc acactaaaaa gccagaatag
      241 gcgggcgcac acgcgctgtc aaatcactgg aatcagcgac agcgcgcagt atgtcagaag
      301 tgcactgcga gcgattgcat gcacgaattc actgcggctc gatgccgacc gcctgcacca
      361 cttggcgata gcgctcgagg tcgcgcttca cgcgcgcgtg gaattccgcg ggcggcatgc
      421 ccatcgtgtc cgcgccgccg acggtaccga aacgctcttt gacgtcggcg agcttcagca
      481 cttccaccgt ctccttgtag agcctgttga cgatgttctt gggagtgcct ttcggcgcga
      541 agagcccgac ccagatcgac gcatcgacgc ccgagatgcc gccttcctgc ggcgtcgggt
      601 aattcgcgtg cgccgagcgc ttcgccgtcg tgatgccgat caccttgaca cgccccgcct
      661 gcagatgcgg cagcacaccc ggaatgccgg cgactgtcat catcacttcg ccagcagccg
      721 tcgcggaaac ggctggcgca ccgcctttgt acggcacgtg cagcagcttc acgcctgcgg
      781 cggaggcgag ccactcggtc gcgaggtggt tgatggagcc cgtgccgggc gaaccgaagg
      841 tcacctgccc cggcttcgct ctcgccagcg cgacgagatc cttgaagccg ttgatcggcg
      901 agttcgcccc gaccaccacc gccatcggcg cgtcactcag catcgtgatc ggcgcgaagt
      961 cgcggtcgag atcgtacggc acgttcctga agatcgccgg attgaccgcg aattcgccgg
     1021 tgtgcgcaga aagcagcgtg tagccgtcgg gtgcgccctt cgccgcgatc tgcaccgcga
     1081 tgaaaccgtt accgccgggg cggttgtcca cgacgaagtt ccagccgagc cgctcggtga
     1141 gcttggtcgt gaggatccgc gtggaggtgt ccaccacgcc cccgggcgcg aacggcacca
     1201 gcacgcggat cgggcgcgtc ggatactcct gcgccaatgc cgcacctgcc agcaagagcg
     1261 acaacgcgag gccgcagaag gcccgcatca atttctcctt catagtatgt ccttccactt
     1321 cgcccgcacc tcgtcctgca gctcgcggct tgcctccgcg acatcgggaa aatcgtcgag
     1381 atgatcgtac ggccggcaag catcgatcac cgccttcgag ctgtagggcg cggcgtagcg
     1441 gtatgcgaca aacatcgggt cgttcttcga gcccatgccg cgcctgatga tgtcgatatc
     1501 cacttcggga tcggtgcggg tcgcgaccgc ccacatgact tcctgcaagt tcgaagggtc
     1561 gatgtcctcg tccaccacca ccgaatagcg cgacatgtag gcaccgacgc cacattggct
     1621 caagatatgc cctgcctgct tcgcgtgccc ggcatagcgc tgattgatcg cgaccacgtt
     1681 gaacatgcgc gccccgccga tctcgtgcgc ccataccgcc ttgacgtccg gcacgccggc
     1741 ggcaatcagc gcgtcgaaca gcagtgccga gcgcatcacc gccttggaat acgagtagtc
     1801 gttcggcggc ttcgcgggcg ggcttccgag gaggatcgga tcgttgcggt aatacacgcg
     1861 ttcgatggtg accaccggcg ccgcgtgcgc cttccccggg tagtagccgt ggaattcgcc
     1921 gaacgggcct tcggtgcgct gggcgcccgg atgtgcccag ccttcgagca cgatttcgga
     1981 tgcagccggg aaagcgagcc cggtgacggg gcccttcgcc accgaaaccg gctcgcccag
     2041 gattgcgccg atatagttgt actcgcacat gccgaagggc acttcgatcc cggagatgag
     2101 atagccgagc ggatcgcagc cgagcacgat cgcgacggga aacggctttc tcgctttcat
     2161 gtacttatcg tactggatcg cgccgtgctt gcccggcacc atgtcgagcc ccacgtggtt
     2221 gcggtcgtgc agctgcacgc ggtaggtgcc cacgttcacc cacgcggagt cgtagtcgcg
     2281 cgtgacgacg cagcacccgg tgccgatgta gcgcccgccg tctttctcgt gccacaacgg
     2341 agagggaaaa gcctcgagat tcacgccgcc gttgtcctgg acgttctcga agacggggcc
     2401 ggtttcggtc tcgacaggat catagcgcgc cgcttcagcc tgccagctct tcggtttgcc
     2461 gcgcagcttg cgcaccagcg catgatggga ggtttcgacg ccgagccgca ggatcgatga
     2521 taggcgcgcg gggctcgccg tgctgcacgt gagcacccgg aaacccttgg ggtagccgac
     2581 gatctcgtcg aagagcagtg ccgggggcag cgccttgcgc acgttgactt cgctgatcgc
     2641 gccgagttca agattccagt gcgcgccgcg cacctctttc agctccccga gtcgacgcgc
     2701 ctcgtcgagc cacacacgaa gatcgagtgg attgcccgcg acagcgctca tgctgcgcgc
     2761 tttcgcttgc gcgcggcgag tttcaccgca tcgatcactt cgggataggt cgcgcaccgg
     2821 cacagattgc cggagagata atggcgaatc tcttcgtccg acggatcggg atgtgcgtcg
     2881 agcaactggc gggtcatcag cacgaatccg ggcgtgcaga atccgcactg gaatgcgctg
     2941 cactcgatgt acgcctgctg aaccgcatcg agtccgtgtc cggcgtcata ttctatggtc
     3001 gtgacttccg ccccatccac gagggcagcg agataaaggc agcccgacac cgccttgccg
     3061 ttcaccagca ccgtgcagca gccgcacaat ccttgcccgc agctctcgcg cgcgccatgg
     3121 agcgcgaatt gccgctgcag catgtccacc aggttgtggt gcggctcgat ctcggcgctg
     3181 acacactcgc cgttcagcgt gaactgaatg gttcgggtca tcgatcgtcc tcgagcggct
     3241 ggccgttcgc ccgccggatc gcgcgataaa ccgcctccgg cgtaatgggc aacgacgtga
     3301 gccgtacccc gaccgcgtcg tgaagcgcgt tcgcgatcgc gggcgacacg ccgaaggtac
     3361 cgctctcgcc cacgcccttc gcgccgaacg ggccgctcgc ctgcgcggcg tcgaccgctt
     3421 ggttctcgat gacgagcgga atgtcgcgca ttcctggaat cttgtactcg gcgagagagg
     3481 cattggtcac ctgccccgcg tcgaaactca tcttctcgga aagcgtgaac ccaagctgca
     3541 tcagcgccgc gcccgagagc tgcgtctcga cgatgcgcgg attcaccgga cggccggcat
     3601 cgacgacgtt gacgagccgc gtcactgtca cgtggccggt ttcggtatcg acttcgactt
     3661 cgacgcccgt tccccccacc atccagaatg gtgtcacgtt gtccgaaaga ccggtctcgt
     3721 ggtcggtcgg cgtgtagctc gggatgaaac ttcccacacc gacgatcttg cccgcctgca
     3781 tgccgtactt ctttctgaag agctcgggaa tgggcgtggt ctcggcggag agctccagct
     3841 cacgtgccag cgcgagcagc ttgtcgcggg cctcgcccgc ggcaagccgc accgcatgcc
     3901 ccatgtggta ggtcgagcgc gagcccaaag tcgccatgtc gtacggcgtc acatcggtgt
     3961 cgggatgaac gaccc
//
LOCUS       DASURG010000417         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1402209, whole genome shotgun sequence.
ACCESSION   DASURG010000417 DASURG010000000
VERSION     DASURG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402209"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..811)
                     /locus_tag="VFY80_07380"
     CDS             complement(20..811)
                     /locus_tag="VFY80_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6007768.1"
                     /translation="MNTPGILIERDHNVGWIRINRPDRLNSFSGTMREDLERALQQME
                     EDPEVRCVIVTGVGRAFSTGGDVSFMKQLIDVSDIATFEHLVRAGAKIVQRIADMSKP
                     VIAAVNGPAAGAGACLALACDLRVASESATIGFGFLRVGLHPDWGGSYFLPRLIGIAR
                     AAEFVLTGEMINAERGERLGIFNRVVAAPELEANARVLAGQMAAAPQRIVADWKRTLR
                     ASLHQELPAVLEMETEAQLRAFRSPDFREGIESFLEKRAPRFGKK"
     gene            complement(808..2013)
                     /locus_tag="VFY80_07385"
     CDS             complement(808..2013)
                     /locus_tag="VFY80_07385"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX6007769.1"
                     /translation="MSKSAYIIDAVRTPIGRHSGVLARVRPDDLAAHAIKATIARSGV
                     DPMRIDDVILGCANQAGEDNRNVARMAALLADLPVEIAGQTVNRLCGSGLQAVNAAAQ
                     AIAAGAGDIIIAGGVESMSRAPYAMLKAEEGFARGAPAIADTVLGWRFINPRLPDHWT
                     IGLGETAENIAEKYHITREAQDEFALRSQRNAGAAARAGRFFDEIVNVELTGRKGEKS
                     IINSDEHPRPETSMEQLARLRPAFREGGTVTAGNSSGLNDGAAALTLVSEERLNQMKA
                     EPLARVVASAVAGVQPHFMGLGPVPATQKALQRAGLQMKDLVLIEMNEAFAAQCLGCM
                     RDLDVNPDIVNVNGGAIALGHPLGCSGARIMTTLVHELRRRGGGYGLATMCIGVGQGI
                     STVVEVFPA"
BASE COUNT          366 a          706 c          645 g          386 t
ORIGIN      
        1 gccacacgtt caatcgcttt catttctttc cgaacctcgg tgcacgcttc tcgaggaatg
       61 attcgatgcc ctcgcggaaa tcggggctgc ggaaggcgcg cagctgcgcc tccgtttcca
      121 tttccagcac agcgggcagc tcctggtgca gggaagcgcg cagcgtgcgc ttccaatcag
      181 cgacaatgcg ctgcggtgcc gccgccatct gtccggccag cacgcgcgcg ttcgcttcca
      241 gctcgggcgc ggcgaccacg cgattgaaga tgcccagtcg ctcgccgcgc tcggcgttga
      301 tcatctcacc cgtcaacacg aactcggcgg cgcgggcaat gccgatcagg cgcggcagga
      361 aatagctgcc gccccaatca ggatgcaggc ccacacgcag aaagccaaaa ccgatggtgg
      421 cactttcact ggccacgcgc aggtcgcagg ccagcgccag gcaggcgccc gcgccggcgg
      481 ccggcccgtt cacggccgcg attaccggct tcgacatgtc ggcgatgcgc tgcacgatct
      541 tcgcgcctgc ccgtaccagg tgctcgaaag tggcgatgtc gctcacatcg atcagctgct
      601 tcatgaagga cacgtcgcca cccgtggaga aggcacgacc gacccccgtg acgatcacgc
      661 agcggacttc cggatcttcc tccatctgct gcagcgcgcg ctccagatcc tcgcgcatgg
      721 tgccggagaa actgttcagc cgatcgggcc ggttgatgcg aatccagccg acgttgtgat
      781 cgcgctctat gagaatgccg ggtgtgttca tgcggggaat acctcgacca cggtggagat
      841 accctggccg acaccaatgc acatcgtggc gaggccgtaa ccgccgccgc ggcgccgcag
      901 ctcgtgcacg agcgtggtca tgatgcgcgc gccggaacag ccgagcggat gcccgagcgc
      961 aatggcgccg ccattcacgt ttacgatgtc gggattcaca tcgagatcac gcatgcagcc
     1021 caggcattga gccgcaaaag cttcattcat ttcgatcaag acgaggtctt tcatttgcag
     1081 accggcgcgc tgcagcgcct tctgcgttgc gggtaccggc cccagcccca tgaagtgcgg
     1141 ctgcactccc gcgaccgcgg atgcgacgac gcgcgccagc ggctcggcct tcatttggtt
     1201 caatctttct tcggaaacca gcgtgagcgc ggccgcacca tcgttcaggc ccgatgaatt
     1261 gccggccgtg accgtaccgc cctcgcggaa agcggggcgg agccgggcca gctgctccat
     1321 gctggtctcc ggacgaggat gctcatcgct gttgatgatg ctcttctcac ctttccggcc
     1381 ggtcagctcg acattgacga tttcgtcgaa gaaacggccc gcgcgcgcgg ccgcgcccgc
     1441 attgcgctga ctgcgcaacg cgaattcatc ctgcgcttcg cgtgtaatgt ggtatttctc
     1501 cgcgatgttc tccgccgttt cgccgaggcc gatcgtccag tgatcgggca ggcgcggatt
     1561 gatgaatcgc cagccgagca cggtatcagc aatggcaggt gctccgcgcg cgaaaccttc
     1621 ttctgccttg agcatggcgt acggggcgcg tgacatggat tcgacgccgc ccgcgatgat
     1681 gatgtcgccg gctccggcgg caatggcctg cgcggccgca ttcacggcct gcaagcccga
     1741 cccgcacaga cgattgaccg tctgcccggc aatctccacg ggtaagtccg ccagcaatgc
     1801 cgccatgcgt gccacgttgc ggttgtcttc accggcctga ttcgcgcagc cgagaatcac
     1861 gtcatcaatg cgcatgggat cgacgccgct gcgcgcgatc gtcgccttga tcgcgtgcgc
     1921 agccagatca tccggccgga cgcgcgccag cacaccgctg tgacggccaa tcggtgtgcg
     1981 tacggcgtcg atgatataag cggatttgct catggctcag ctgaggtcga cccacacact
     2041 cttgatctgc gtgtacagat cgagtgcgta tgcgcccagc tcgcgtccgt agccggactg
     2101 ctt
//
LOCUS       DASURG010000418         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_890377, whole genome shotgun sequence.
ACCESSION   DASURG010000418 DASURG010000000
VERSION     DASURG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890377"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="VFY80_07390"
     CDS             <1..1778
                     /locus_tag="VFY80_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6007770.1"
                     /translation="EAQADELIRMKLAFAFGRIRPSEELVQPVYVEREAVEIRNGVNI
                     RRLRTNVFEISHGEDRVEVDLNLAPGQMYECPYTLGNHLLQRDYFAVVHTGDGDGWDM
                     NRPAMGSIVLFQGRVYLIDAGPNIDYALTALGIGVNEIDGIFHTHCHDDHLVGLTALI
                     RGDRRIAYYAVPMVRASVVKKLETVMRISESDFSALFEVHDLELEEWNDIEGLEVKPI
                     LSPHPVETTIFYFRAMWEGGNRSYAHLADIASFDVLRKMIAPDHATAGISRARFEKTV
                     RDYTQRVDVKKIDIGGGIIHGAAIDFRGDPSGKLILAHTARRLTEQERAIGSGAPFGT
                     VDVLIEGISDELRRRAFGYLRDYFPEVPIHRIRHLMNNRILVMNPEVLLVKEGQPVAD
                     IYLILSGAVEMLRTGMPGSHLLSAGSIIGETPALLDTRAEETYRAVSFVQAMRLPRDL
                     YLDFVKRNNLYQQIVESRDELEFLRSSWLFADGVSCMTLNRLVRATHPVSFEKDRTLA
                     PPPDELIVMRSGEALFTTAAGYEEPLSTGSYFGATALLNHVNHGSRVRFMRASDAYQV
                     PLTSILDVPVVRWKMLETHRRRYEE"
     gene            complement(1779..>2023)
                     /locus_tag="VFY80_07395"
     CDS             complement(1779..>2023)
                     /locus_tag="VFY80_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX6007771.1"
                     /translation="ARLEFGPITAAWYSELGGLNKFVHIWPYPTLDARNETRKKAQAA
                     GAWPPSVAAKKAGLEVYHLLKQENKIVMPAAFSPLQ"
BASE COUNT          368 a          656 c          639 g          360 t
ORIGIN      
        1 cagaggccca ggccgacgaa ttgatccgga tgaagcttgc attcgcgttc ggccgcatcc
       61 gcccgagcga ggagctggtg cagccggtat acgtcgagcg cgaggccgtg gagattcgca
      121 acggcgtcaa catccggcgc ctgaggacca acgttttcga gatctcgcac ggcgaggacc
      181 gcgtcgaggt ggacctcaat ctcgcgccgg ggcagatgta cgaatgcccc tacacgctcg
      241 gcaaccacct cctgcagcgc gattattttg cggtcgtcca taccggagac ggcgacggct
      301 gggacatgaa tcgccccgcg atgggcagta tcgtgctgtt ccaggggcgc gtgtacctga
      361 tcgatgccgg ccccaacatc gactatgcac tcacggcgct cggcatcggc gtcaacgaga
      421 tcgatggcat tttccacacg cactgccacg acgaccatct cgtcgggctg acggcactga
      481 tccgcggcga ccgccgcatc gcgtattacg cggtgcctat ggtgcgcgct tcagtggtga
      541 agaagctcga gacggtcatg cgcatctccg aaagcgactt cagcgcactt ttcgaggtgc
      601 acgatctcga gctcgaagag tggaacgaca tcgaggggct cgaagtgaag cccattctct
      661 cgccgcatcc agtcgagacc acgatcttct atttccgggc gatgtgggaa ggcggcaatc
      721 ggagctacgc gcatctcgcg gacatcgctt cgttcgacgt gctgcgcaag atgatcgccc
      781 cggaccatgc caccgcgggg atcagccgag cgcgcttcga gaagaccgtg cgcgactaca
      841 cccagcgcgt ggacgtgaag aagatcgaca tcggcggcgg gatcatccac ggcgccgcga
      901 ttgatttccg cggtgacccc tcggggaagc tcatccttgc ccacactgcg cggcgcctga
      961 ccgagcagga gcgcgccatc gggtccggcg cgcccttcgg cacggtggac gtgctgatcg
     1021 aaggcatatc cgacgagctg cgccggcgcg cattcggtta tctgcgcgac tacttccccg
     1081 aagtgccgat ccaccgcatc cggcacctca tgaacaaccg gatcctagtg atgaatccgg
     1141 aagtgctgct ggtgaaggaa gggcagccgg ttgccgacat ctatctcatc ctgagcgggg
     1201 ccgtggagat gttgcgcacc ggcatgccgg gaagccatct gctttcggcc ggttccatca
     1261 ttggggagac gccggcgctg ctcgacacgc gcgctgaaga aacctaccgc gccgtgagct
     1321 tcgtccaggc gatgcgactc ccgcgcgatc tctatctcga cttcgtcaag cgcaacaacc
     1381 tgtatcagca gatcgtcgag tcgcgcgacg agctggaatt cctgcgctcg agctggctct
     1441 tcgccgacgg cgtctcatgc atgacgctca accggctcgt gcgtgcgacg caccccgtct
     1501 cattcgagaa agaccgcacg ctcgcgcccc cgccggacga gctgatcgtg atgcgttccg
     1561 gggaggcgct gttcaccacc gcggcggggt atgaagagcc gctctcgacc ggaagctact
     1621 tcggcgcgac tgcgctgctc aatcacgtga atcacggctc gcgggtgcgt ttcatgcgcg
     1681 cgtcggacgc ctaccaggtt ccgctcacga gcatcctcga tgtgccggtg gtgcgctgga
     1741 aaatgctcga gacgcaccgc cggcgctacg aagagtagct actgcagcgg cgagaaggcc
     1801 gccggcatga cgatcttgtt ttcctgcttg agcaggtgat acacctcgag ccccgccttc
     1861 ttggcggcga ccgagggtgg ccaggcaccc gccgcctgcg ccttcttgcg cgtctcgtta
     1921 cgggcatcga gcgtgggata cggccagatg tggacgaact tgttgagccc gcccagctcc
     1981 gaataccacg ctgcggtgat cggcccgaac tcgagccgcg cgg
//
LOCUS       DASURG010000419         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_279232, whole genome shotgun sequence.
ACCESSION   DASURG010000419 DASURG010000000
VERSION     DASURG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279232"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFY80_07400"
     CDS             complement(<1..691)
                     /locus_tag="VFY80_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007772.1"
                     /translation="MASLWLNPSAVARALGAAAFILVVLHLAGQFSKLALGHGSLKGL
                     VPLFNLSAEQNIPAYFSVLLILLAALILAGIAAINHGQNMPHTSKWTLLSIGFLCMAF
                     DEAFSIHERLMVPLRAMMGVSDAVGTVKGEVVLGIFYYAWVIPGMVIVLLLGLYFLRF
                     LLYLPARTRYRFLCAAVLYVGGAIGFELIGGAYAEVHGYDNWTYTFIATTEEVLEMAG
                     VICFIWALLKYC"
     gene            complement(793..1050)
                     /locus_tag="VFY80_07405"
     CDS             complement(793..1050)
                     /locus_tag="VFY80_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX6007773.1"
                     /translation="MKVHIPSALRSYTERSDTKASGATLAAVLADLDRQYPGIRFRMI
                     DEQERIRRHIRIFVNGEQIRVLSHPLRESDEVVIVQALSGG"
     gene            complement(1061..2185)
                     /locus_tag="VFY80_07410"
     CDS             complement(1061..2185)
                     /locus_tag="VFY80_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX6007774.1"
                     /translation="MSDTLVILVATRKGAWLFHGESARRSWRVDGPHFLGHVVSHIVL
                     DPRDRRTLLAAAKTGHLGPTIFRSTDLGRSWKEAAQPPAFAKAADGTGRAVDHTFWLT
                     PGPASERDVWYAGTSPQGLFRSEDGGVTWAPFSSINDDPKYREWMGTVQDGTPDGPKL
                     HSIIVDPRDPKHLYFAMSGGGVHESRDGGRTFHPLLKGLEVVEGFDGTQPTFHDPHCV
                     RLCPSNPDRLYQQNHCGIYRLDRPSDTWVRIGKSMPAEVGDVGFPMVVHPRNADTAWV
                     LPMDGTSVWPRTSPGGKPAVYATRDGGTTWQRLNAGMPEEQAWWTVKRQAMTADALDP
                     VGLYFGTTSGELWISRDEGREWRCMARHLPEIYAVETALA"
BASE COUNT          437 a          727 c          744 g          418 t
ORIGIN      
        1 tgcagtattt caacagcgcc cagataaagc agatgacgcc cgccatctcg agcacctctt
       61 ccgtcgtcgc gatgaaagtg taggtccagt tgtcgtagcc gtgcacttcg gcataagcgc
      121 cgccgatcag ctcgaaaccg atggcacctc cgacgtagag aaccgctgcg cacagaaacc
      181 tgtagcgcgt gcgcgccggg aggtacagga ggaatcgcag gaagtagagc cccagcagca
      241 gcacgatgac catgccgggg atgacccagg cgtaatagaa aattccgaga accacttccc
      301 ctttgacggt gcccacggca tcacttaccc ccatcatcgc ccggagcggc accatcagcc
      361 tttcgtgaat cgaaaaagcc tcgtcgaaag ccatgcaaag aaatccgatc gacagcaagg
      421 tccacttgga cgtatgaggc atgttctggc catgattgat ggcggctata ccagccagaa
      481 tcagcgcggc aagcaggatg agcagcacgg agaagtacgc cggtatgttc tgttctgcgc
      541 tcaggttgaa gagcggcacg aggcccttca aggagccgtg gccgagcgcg agtttcgaga
      601 actggccggc caggtggagc actaccagta tgaacgccgc ggcgcccaat gcgcgggcaa
      661 ccgctgacgg attaagccac aggcttgcca cgcgccaccg ggagatgata cgagccgagc
      721 aggctaaccg cagggggtga gcgcttgggc tgcgaacagt gccgccggcg tggccctttt
      781 ggactatgcg gcttaaccgc cgctcagcgc ctgcacgatc acgacttcgt cgctttcgcg
      841 tagcggatgc gagagcaccc ggatttgctc gccattcacg aaaatgcgga tgtggcgccg
      901 gatgcgctcc tgctcgtcga tcatgcgaaa acgtatgccg gggtactggc gatcgagatc
      961 ggcgagaacc gctgcgagcg ttgcgccgct tgctttcgtg tcgctgcgct cggtataaga
     1021 gcgcagcgca ctcggaatat gcaccttcat ggcgtagccg tcaggcgagc gcagtctcca
     1081 cggcgtagat ttccgggagg tgtcgcgcca tgcagcgcca ctcgcggcct tcgtcgcgac
     1141 tgatccagag ctctccgctg gtcgttccga agtagagccc tacgggatcc agagcgtccg
     1201 cggtcatggc ctggcgcttg acggtccacc aggcctgctc ttccggcatg ccggcgttca
     1261 ggcgctgcca cgttgtaccc ccgtcgcgcg tcgcgtagac cgcgggcttg ccgcccggac
     1321 tcgtgcgcgg ccaaacgctg gtaccgtcca tgggcagcac ccacgcggta tcggcattgc
     1381 gcggatgcac caccatggga aacccgacat cccccacttc ggctggcatg ctcttgccga
     1441 tgcgtaccca agtatcggaa ggacgatcga gacggtagat cccgcagtga ttctgctggt
     1501 agagacggtc gggattgctc gggcagaggc gcacgcagtg tggatcgtga aacgtcggct
     1561 gcgtgccatc gaatccttcg accacttcca ggcccttgag caacggatgg aacgtacgcc
     1621 cgccatcgcg cgactcgtgt acgccaccgc ccgacatcgc gaaatagaga tgcttcggat
     1681 cgcgtgggtc gacgatgatg gagtgaagtt tcgggccatc cggcgtgccg tcctgcaccg
     1741 tccccatcca ctcgcggtat ttcgggtcgt cgttgatgct ggaaaagggc gcccacgtga
     1801 cgccgccgtc ttccgatcga aagagtccct gcggagacgt gcccgcgtac cagacatcgc
     1861 gctcggatgc gggaccgggc gtcaaccaga acgtgtgatc gacggcgcgg ccggttccgt
     1921 ccgccgcttt ggcgaacgcc ggcggctgcg ccgcttcctt ccagctgcgg ccgaggtcgg
     1981 tcgagcggaa gatagtcggg ccgaggtggc cggtcttggc ggcggcgagc agcgtgcgcc
     2041 ggtcgcgcgg gtccagcacg atgtggctga cgacgtgacc caggaaatgc gggccgtcca
     2101 cgcgccacga ccttcgcgcg gattcaccgt ggaaaagcca cgcgcctttt cttgtggcaa
     2161 cgaggatgac aagcgtatcg ctcatgggtt tttcacctcc ttttgcgtcc cgctatggcg
     2221 ggcggcgacg gtttcccgta tcgggcggat cggccgcact tcgatgctcc cgacgtgcgc
     2281 gggcggtatc cgggaagcga tctggatcgc ttcattgaga tctcgc
//
LOCUS       DASURG010000420         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_482379, whole genome shotgun sequence.
ACCESSION   DASURG010000420 DASURG010000000
VERSION     DASURG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482379"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFY80_07415"
     CDS             complement(<1..823)
                     /locus_tag="VFY80_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6007775.1"
                     /translation="MPTQVKRKARLLVVDDDPGLLRLLTIRLRAENYEVDAVESAAAA
                     LAALGRVRPDLVITDLRMDQMDGIGLLKEIQSRAPGLRVIILTAHGTIPDAVQATQSG
                     AFAFLTKPVDKQELLDQVGKALKVSGFADTTEDWRSEIVTRSAIMEERLAQAHMVAGT
                     DARVMITGESGTGKELLARAIHRASPRRNRPFVAINCSAMAENLLESELFGHVKGAFT
                     GAIREHRGLFQAADGGTLMLDEIGDMPPRLQVKLLRVLQENVIRPVGSTDAIAVNV"
     gene            complement(780..1433)
                     /locus_tag="VFY80_07420"
     CDS             complement(780..1433)
                     /locus_tag="VFY80_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007776.1"
                     /translation="MLRLCVVASLCSVLAGCELAGRLGNDPLPAPIVAPAQISSSGLR
                     AYLDTMEGLASADPARQADVFYEVEREYTRAPTTAATLRYAVALVTPGHPATKPLEGK
                     RQLETLLATPERMTQEERTFAAVLLHETNARLKLEAENRRLLATLDDRSRSQANSDKR
                     VQAQLEENARLRRALAEAEQKLNAIKEIERSIIERSPSPPGSRDANSSETQSSSTSR"
     gene            complement(1436..>2115)
                     /locus_tag="VFY80_07425"
     CDS             complement(1436..>2115)
                     /locus_tag="VFY80_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6007777.1"
                     /translation="SHELKTPLASIREGTELLMDGAVGELDSAQREVTTILRDNGIKL
                     QQLIENLLSFSAWQARHSGLEISEFRLRPLIKSALETHQLTLLAQRVHLDLKVQDVEL
                     RADRAKLKLILDNLLSNALKYSPRGGTIFIHAHADKDLLVLDVADTGPGISKEERSAI
                     FEAFYSGRAPTAGHLKGTGIGLSVVSEFVQAHGGSIEILDGVFPGAHFRIRLPLAPHL
                     EAEAEPA"
BASE COUNT          331 a          693 c          692 g          399 t
ORIGIN      
        1 gcacgttcac cgcgatcgcg tcggtgctgc cgacggggcg aatcacgttc tcctgcagca
       61 cgcgaagcag cttgacctgc aggcgcggcg gcatgtcgcc gatctcatcg agcatcagcg
      121 tgccgccatc ggcggcctgg aagaggccgc ggtgctcgcg aatcgcgccg gtgaaggcgc
      181 ctttcacgtg gccgaacagc tcgctttcca gcagattctc ggccatcgcg ctgcagttga
      241 tcgcgacgaa cggccggttg cggcgcgggc tggcgcgatg aatcgcacgg gccagcagct
      301 ctttgccggt gccgctctcg ccggtgatca tgacgcgcgc gtcggtgccc gcgaccatgt
      361 gagcctgcgc cagccgctct tccatgatcg cgctgcgcgt gacgatctcg ctgcgccagt
      421 cttcggtggt gtcggcgaag ccggaaacct tgagcgcctt gccgacctgg tcgagcagtt
      481 cctgcttgtc gaccggcttg gtgaggaatg cgaaggcgcc gctctgcgtc gcctgcaccg
      541 catccggaat cgtgccgtgc gcggtgagga tgatgacgcg cagcccgggc gcgcggcttt
      601 gaatttcctt caacagcccg atgccgtcca tctggtccat gcgcaggtcc gtgatcacga
      661 gatccggacg cacgcgcccg agcgccgcca gagcagcagc cgcgctttcg accgcatcga
      721 cttcgtagtt ttccgcgcgc agacggatcg tgagcagccg cagcagcccc ggatcgtcgt
      781 caacgactag tagacgagct ttgcgtttca cttgagttgg catcgcgact gccagggggc
      841 gagggactgc gttcaatgat ggacctctcg atttctttga tcgcgttgag cttctgctcg
      901 gcctcggcga gggcgcggcg caggcgcgca ttttcctcaa gttgagcttg cacccgcttg
      961 tccgagtttg cctgcgaccg gctgcggtcg tcgagtgtcg ccagcagccg acgattctcg
     1021 gcctcgagct tgaggcgcgc gttcgtctcg tgcagcaaca ccgctgcgaa agtacgctct
     1081 tcctgcgtca tccgttccgg cgtcgcaagc aaagtttcca attgacgctt gccttccagc
     1141 ggcttggtcg ccggatgccc gggcgtgacc agtgccaccg cataacgcaa cgtggccgca
     1201 gttgtcggcg cacgcgtgta ctcacgctcg acttcataga acacgtcggc ctggcgcgcg
     1261 gggtccgcgc tcgcgagtcc ttccatcgtg tcgaggtagg cgcgcagacc gctcgaggag
     1321 atctgcgccg gcgcgacgat gggtgccggc agcggatcgt tgccgagcct gccggcgagc
     1381 tcacagccgg cgagcacact gcacagcgac gcgacgacgc agaggcgcag catggctacg
     1441 cgggctccgc ttccgcttcc aggtgcggag cgagcggcag tcggatgcgg aaatgcgctc
     1501 cgggaaacac gccgtccaag atttctatcg agcctccgtg agcctgcacg aattccgaga
     1561 cgaccgacag gccgatcccg gtgcctttca gatggccggc ggtgggcgcg cggccggaat
     1621 aaaacgcttc gaaaatcgca ctgcgctctt ccttcgagat gcccgggccg gtatcggcga
     1681 cgtcgagcac cagcaggtct ttgtccgcgt gcgcgtgaat gaaaatagta ccgccgcgcg
     1741 gactgtactt gagcgcgttc gacagcagat tatccaggat cagtttcagt tttgcgcggt
     1801 ccgcgcgcaa ttccacgtcc tgcaccttca ggtcgaggtg cacccgttgc gcgagcagcg
     1861 tcaactggtg cgtctcgagc gccgacttga tcagcggccg cagccggaat tcgctgatct
     1921 cgagcccgga gtggcgcgcc tgccaggcgc tgaaggacag caggttctcg atgagctgct
     1981 gcagcttgat gccgttgtcg cgcaggatcg tcgtcacctc gcgctgcgcg ctgtccagct
     2041 cgccgaccgc tccgtccatc agcagctccg tgccttcgcg gatgctggcg agcggcgtct
     2101 tgagctcgtg cgaca
//
LOCUS       DASURG010000421         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1198050, whole genome shotgun sequence.
ACCESSION   DASURG010000421 DASURG010000000
VERSION     DASURG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198050"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1677
                     /locus_tag="VFY80_07430"
     CDS             208..1677
                     /locus_tag="VFY80_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX6007778.1"
                     /translation="MRNPASMRFAASLVALLMAACSGSENPGINQANASQAQGSAAAA
                     APPVAAPTRALPDFSALVERVGPAVVNVSTIRTPQNGSSQTPGMPPGAENDPFFEFFR
                     RFGIPFDMPQPSPTMGLGSGFIISEDGYILTNAHVVKGATEVTVRLTDRREFKAKIVG
                     ADARSDVATLKIEATGLPTVRIGNPDTVKVGDWVAAIGSPFGFENSVTAGIVSAKSRS
                     LPDSTYVPFIQTDVAVNPGNSGGPLFNMNGEVIGINSQIYSRTGGYMGLSFAIPINVA
                     MKVRDDLVRYGKVTRGRIGVTIQDVNQALAKSFGLEKPTGAIVSSVEKGGPADKAGVQ
                     PGDIILRVGKEQVERSGELPNLVAGLQPGTKTEIVVWRNGKQTTLPLTVGELNETNVA
                     SAAGGGSESGGKLGLAVRPLSPAERKQLESEGGVVVENAQGPAARAGIRPGDVILGVN
                     GKRIENPQELRDAVEKSDGVVALLVQRSDARIFVPVPLG"
     gene            complement(2016..2984)
                     /locus_tag="VFY80_07435"
     CDS             complement(2016..2984)
                     /locus_tag="VFY80_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6007779.1"
                     /translation="MLNAAIVGIGWWGKNLVNAVQDKSERIRFVHGVSKEPETVREFA
                     SSHGFRLSTDLEEALADPRVEALVLATPHSLHADQIVRVAAAGKPVFCEKPLSLTRAD
                     AERAIAATKRAGIPLGVGQNKRFWPSTQALVRTVRSGVLGKALHIEGHYSNENSSMHF
                     SEWRADPRETPGAGMTGTGIHLLDVFSTLAGAAREVNAQFVSTKPSPDPLDTVSALFK
                     FENGMSGMLGAVRATPIYWRVHVFGDQGNAEALGETELVLRLKGKKIESQRFEPLDSI
                     RAELEAFADAAAGRAPYPITPDEMLTTVATFEAVIQSIARGAAVRL"
     gene            complement(3013..3540)
                     /locus_tag="VFY80_07440"
     CDS             complement(3013..3540)
                     /locus_tag="VFY80_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3256..3258),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX6007780.1"
                     /translation="MTTTQWLRDPTAETTALKRGRTKPPKSLDGLTIGLFDIGKTRSD
                     EFLDRVETRLAEHKLKVRRYAKPTNAKVAPPEVIDHVANEADVVLIALSDUGSCTSCS
                     LHDLMTLDARGVPGISVITAEFRNALEAQSKALGFEPAVLYVPHPIQNRTPEELKRIA
                     DDIIEPALNMLVSNR"
BASE COUNT          643 a         1132 c         1155 g          648 t
ORIGIN      
        1 aacatgcacg attggcgcga ggcgatcgat cagtcgaagg acacggtggc gctgctcgtg
       61 caacgcgaca ccaagcgcgt ctacgtccct gtcacgccgg gctagccggt ccacaacttt
      121 ttttcgtttg agggtgtcaa agcctgcgac tgcccccggg cggtgaactg ccgcccgcga
      181 gaggcttttt gtgacaggag gatacggatg cgaaacccag cttccatgcg gttcgccgcg
      241 agcctcgtcg ccctcttgat ggcggcctgc tccggcagcg agaaccccgg catcaaccag
      301 gccaatgcaa gtcaggcaca aggcagcgct gcggctgcgg ccccgcccgt tgcggcccct
      361 acccgcgcgc ttcctgattt ttcggcgctg gtcgagcgcg tggggccggc ggtggtcaat
      421 gtcagtacca ttcgcacgcc ccagaacggc agcagccaga cacccgggat gccccctggt
      481 gccgaaaacg atccgttttt cgagttcttc cggcgcttcg gcataccgtt cgacatgccg
      541 cagccttcac ccaccatggg cctggggtcg ggtttcatca tcagcgaaga cgggtacatc
      601 ctgacgaacg cgcacgtcgt gaaaggcgcg accgaagtga ccgtccggct cactgaccgc
      661 cgcgaattca aggcgaagat cgtaggcgcg gacgcgcgca gcgacgtggc gacgctgaag
      721 atcgaagcaa cgggactgcc gaccgtaagg atcggcaacc ctgacacggt caaggtgggc
      781 gattgggtgg cggcgatcgg ctcgccgttc ggattcgaaa acagcgtgac ggccggcatc
      841 gtgagcgcaa aatcgcggag cctgcccgac agcacttacg tgccgttcat tcaaaccgac
      901 gttgcggtca atcccggaaa ctccggcggg ccgctcttca acatgaatgg cgaagtgatc
      961 ggcatcaatt cccagatcta cagccgcacc ggcgggtaca tggggctgtc attcgcgatc
     1021 ccgatcaacg tggcgatgaa agtgagggac gacctcgttc gctacggtaa agtgacgcgc
     1081 gggcgcatcg gcgtcaccat ccaggacgtg aaccaggcgc tcgcgaagag cttcggactc
     1141 gagaagccga cgggcgcgat cgtcagttca gtggaaaaag gcggcccggc cgacaaggcg
     1201 ggcgtgcagc ccggcgacat catactgcgc gtggggaagg agcaggtcga acgctccggt
     1261 gagctgccga atctcgtggc gggactgcaa cccggcacga agacggagat cgtcgtgtgg
     1321 cgcaacggca agcagacgac actgccgctc actgtcggcg agctcaacga aaccaacgtc
     1381 gcctccgcgg cgggtggagg cagcgagtcg ggcggcaagc tggggcttgc ggtgcggccg
     1441 ctcagtcctg cggagcgcaa gcagctcgag tccgagggtg gcgtcgtggt ggagaacgcg
     1501 caaggccccg ccgcgcgcgc gggcatccgt ccgggcgatg tcatattggg tgtgaatggg
     1561 aagcgcatcg agaatccgca ggagctgcgc gacgccgtcg agaagagcga tggcgtggtc
     1621 gcgctcctcg tgcagcggag cgacgcgcgg atcttcgtgc cggtgccgct cggctaagct
     1681 tggctttcgc gcttggggaa actcagcagg aacccgccat tcgcgggtag accgcctccg
     1741 caggaaaccg actgccatgc gtcgttccac cgcctatgca cgaaaggact gcggttcgac
     1801 cgagcgaagc gaaggccgag aaaaaagaac tcgtcacacc ggcgaaaacc ggtgtccagt
     1861 atgacgtcct ccactggatt ccggctttcg ccggaatgac gaggctgctt tggaactgcc
     1921 gggtggcttt cgcgggaatg acgatgcttt gacgcgccgg cggtggcctt cgccggaatg
     1981 acgatcttcg tcgaccggct tttttcaaga gaggtttaga ggcggacagc tgcgccgcgc
     2041 gcgatcgact ggatcaccgc ttcgaaggtg gcgacggtcg tcagcatttc atccggcgtg
     2101 atggggtacg gggcgcggcc cgctgccgca tcggcgaacg cttcgagctc ggcccggatg
     2161 gaatcgagcg gttcgaaacg ctgactctcg atttttttgc ccttgagccg cagcacgagc
     2221 tccgtctcgc caagcgcttc ggcattgccc tggtctccga aaacgtgcac acgccagtaa
     2281 atgggtgtcg cgcgcaccgc accgagcatg ccggacatgc cgttctcgaa cttgaacagc
     2341 gcagatacgg tgtcgagcgg atcgggtgac ggtttcgttg agacgaactg tgcgttcacc
     2401 tcacgcgccg ctccggccag cgtgctgaag acatccagca agtggatgcc ggtgcccgtc
     2461 atcccggcac ccggcgtctc gcgcgggtcc gcacgccact cggaaaagtg catgctggaa
     2521 ttctcgttgc tgtaatgtcc ctcgatgtgc agcgcttttc caagcacgcc actcctcact
     2581 gtcctgacga gcgcctgcgt ggaaggccag aagcgcttgt tctgccccac gccgagcgga
     2641 atccccgcgc gcttcgtcgc cgcaatcgca cgctcggcgt cggcgcgcgt gagcgaaagc
     2701 ggcttctcgc agaataccgg cttgccggca gcggcaacgc gcacgatctg atcggcgtgc
     2761 agcgaatgcg gcgtcgccag cacgagcgcc tctacccgcg gatcggcaag cgcttcctcg
     2821 aggtcggtgc tgaggcgaaa gccgtggctg gaggcgaact cccgcaccgt ctccggctcc
     2881 ttgctcaccc cgtgcacgaa ccggatgcgc tcgctcttgt cctgcactgc gttgaccaaa
     2941 ttcttgcccc accagccgat gccaacgatt gccgcgttca gcattgccac tccttttgcg
     3001 ttgcgccgcc tctcatcgat tggagaccag catgttgagc gccggctcga tgatgtcgtc
     3061 ggcgatgcgc ttcaattcct cgggcgtgcg gttctggatc ggatgcggaa cgtacaacac
     3121 cgcaggctcg aatccgagcg ccttcgattg cgcttcgagc gcgttgcgga attccgccgt
     3181 gatcaccgag attcccggca cgccgcgcgc gtcgagcgtc atcaagtcgt gcaaactgca
     3241 cgacgtgcac gaccctcaat ccgacaacgc gatcaacacg acgtctgctt cgttcgcgac
     3301 gtggtcgatc acttccggcg gtgcgacctt ggcgttggtc ggcttggcgt agcgcctgac
     3361 tttgagcttg tgttctgcaa gacgcgtctc gacccgatcc aggaattcgt cgctgcgggt
     3421 cttgccgatg tcgaagagcc cgatggtcag tccgtcgagc gacttgggcg gcttcgtgcg
     3481 cccgcgcttc aaagcggtcg tttcagctgt gggatcgcgc agccattgcg tggtcgtcat
     3541 gctcgcacct ccttcgttac cggttgcagt tcttcgcg
//
LOCUS       DASURG010000422         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_176959, whole genome shotgun sequence.
ACCESSION   DASURG010000422 DASURG010000000
VERSION     DASURG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176959"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFY80_07445"
     CDS             <1..560
                     /locus_tag="VFY80_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6007781.1"
                     /translation="LPMTAAQILWVNLVTSVTLGLALAFEPPEPGVMQRPPRPPSAPL
                     LSGFLVWRVVLVSFVFFAACQGIFVYAIERGDDLETARTLVVNTLVVLEAFYLFNVRY
                     LHMTSITFRGALGTPAVLYALGALVIAQLVLTYAPFMHEWFQTRALALLDGILILAIG
                     VTVMMLLELEKALFRRLGVLARAAI"
     gene            729..1832
                     /locus_tag="VFY80_07450"
     CDS             729..1832
                     /locus_tag="VFY80_07450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6007782.1"
                     /translation="MKIFKLPDLGEGLQEAEIVKWHVAVGEEVKADQPMVSVETAKAL
                     VDIPAPYSGRIARLYGTPGEIAHIGAPLVAFEGEGDAPDAGTVVGKVEVGQKVVAAAP
                     AAAVPAAGGIKATPAVRALARKLNVDLTMVTPTGADGLITANDVQRAAKVLAETGPIE
                     LLRGVRRVMAQNMELANAEVASATIMDDADIDAWPSVANVMLRLIRALVAGCRAEPAL
                     NAWYDSEKIGRWVLKKVDIGIAVDTPDGLLVPVLRNVESRSVEDLKRGLERLVADARA
                     RKVPPEELRGNTITLSNYGTIAGRYASPVVLPPTVAILGAGKIAKQVIVADDRPAVHR
                     MLPLSLTFDHRVVTGGEAGRFLNAVIADLQLPE"
     gene            complement(1839..2087)
                     /locus_tag="VFY80_07455"
     CDS             complement(1839..2087)
                     /locus_tag="VFY80_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007783.1"
                     /translation="MKAVELGELLESLGVPILGYRENDGYLGASVRVTDNVHIEIAEE
                     GAISVVAHNARGLQLYAARTEVNELICDLVEAGALVLH"
BASE COUNT          431 a          811 c          823 g          465 t
ORIGIN      
        1 cgctgccgat gacggccgct cagatcctct gggtcaatct cgtaacgtcg gtaacgctcg
       61 gcctcgcgct cgcgttcgag ccgcccgagc cgggcgtgat gcagcgtccg ccgcggcccc
      121 ccagcgcccc gctcctgtcg ggctttctcg tatggcgcgt ggtgctggtt tccttcgtct
      181 tcttcgcggc atgtcagggc atattcgtat acgccatcga acgcggcgac gacctcgaga
      241 ctgcacgcac gctcgtcgtc aatacgctgg tggtactgga agcgttctat ctcttcaatg
      301 tgcgctatct gcacatgact tcaatcactt ttcggggcgc gctcgggaca ccggccgtcc
      361 tttacgcgct cggtgcactc gtcatcgcgc agttggtcct cacctatgca cctttcatgc
      421 acgagtggtt ccaaacccgg gcgctggcgc tgctcgacgg catcctgatc ttggcaatcg
      481 gcgtaacggt catgatgctt ctggagctcg aaaaggcgct cttccgccgt ctcggcgttc
      541 tggctcgcgc agcgatatag cagacctggg gctccggcca gccgggctac gccgacatgc
      601 gcctcatgtt ccccgcgcaa cgctcccgga gagctatgcg cagggttgtt ttgtcatgcg
      661 cggcgcggga agctattgct gctatattcg cggcgaagtg cgaatgcctg cgggacggag
      721 aaggaacgat gaaaatcttc aagctgcccg acctgggcga aggcttgcag gaagcagaga
      781 tcgtcaagtg gcatgtcgct gtcggcgagg aagtgaaggc ggaccagccg atggtttcgg
      841 tcgaaacggc gaaggcgctc gtcgatatcc cggcgcctta ctccggccgc atcgccaggc
      901 tctacggcac gccgggagag atcgcgcaca tcggcgcgcc gctcgtggca tttgaaggcg
      961 aaggcgacgc gccggacgcc ggcacggtgg tcggcaaggt cgaggtcgga cagaaagtgg
     1021 tcgcggcggc gcccgcggca gccgtccctg cagcgggcgg catcaaagcg acgccggccg
     1081 tgcgcgcgct ggcgcgcaag ctcaatgtag atctcacgat ggtgacgccg accggcgcgg
     1141 acggcctcat caccgccaac gacgtgcagc gcgcggcgaa agtgctcgcg gaaacgggac
     1201 ccatcgaact gctccgcggg gtgcggcgcg tgatggctca gaacatggag cttgcgaacg
     1261 ccgaagtcgc atcggcgacg atcatggacg atgccgacat cgacgcgtgg ccgtcggtag
     1321 cgaacgtgat gctgcgcctc attcgggcgc tcgtcgcggg atgccgcgcg gagcctgcgc
     1381 tcaacgcctg gtacgacagc gagaagatcg gccgctgggt gctgaaaaag gtcgacatcg
     1441 gcatcgcggt cgatacgccg gacggattgc tcgttccggt gctgcgcaat gtcgaatcgc
     1501 gcagcgtcga ggatctcaag cgggggctcg agcggctcgt ggccgacgcg cgggcacgca
     1561 aggttccgcc cgaggagctg cgcggcaaca ccatcacgct gtcgaactac ggcaccatcg
     1621 cgggccgcta tgcgtctccg gtcgtgctgc cgccgactgt cgccatactg ggcgcaggaa
     1681 agattgcgaa gcaggtgatc gtcgcggacg acaggcccgc cgtgcaccgc atgctgccgc
     1741 tctcgctaac gttcgatcac cgtgtcgtga cgggcggaga ggcggggcgt ttcctcaacg
     1801 ccgtgatcgc ggacctgcag ttgccggagt aggcccgctc agtgaagcac taaggcgccc
     1861 gcttccacga gatcgcaaat gagctcgttc acttccgtac gggcagcgta gagctgcagc
     1921 cctctcgcgt tgtgcgccac caccgagatg gcaccttcct cggctatctc gatatggacg
     1981 ttatcggtaa cgcggaccga tgcgccgagg tagccgtcgt tctccctgta gccgagtatc
     2041 ggtacgccga ggctctccag aagctcccca agctccactg ctttcattgt cgccctcccc
     2101 agggtttcga caagggacag gtttatcacg cccggggaag ctgcacccgc tcttttcgtg
     2161 gccgccccga tgaacggtat gttccggctg acggccgctg tggcacaaaa gctgcgacag
     2221 gcgcgcctag cggttcgttt gaagattggc gcgggcgcca cgatgtcgtc atgccggcga
     2281 aagccgcgac ccactaagac tttgatttgt taggcgcacc ggttttcacc ggtgtgacga
     2341 gctccctttc aacggcctgc tagcgttcga ccgggagctt gcggtcgttc caatccacca
     2401 tgccgtcctt caagttgtag acccgttcga agcccagccc gtagagcatc gcggcagccg
     2461 tagtgctgcg cacgcccgaa cggcacacac cgttgtacga agacctgctg gcagggcccg
     2521 cggtggcggc
//
LOCUS       DASURG010000423         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1198601, whole genome shotgun sequence.
ACCESSION   DASURG010000423 DASURG010000000
VERSION     DASURG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198601"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFY80_07460"
     CDS             complement(<1..384)
                     /locus_tag="VFY80_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX6007784.1"
                     /translation="MAAQPFHDRDGWVWFDGEWVPQREAKVHVLTHAMHYASCVFEGE
                     RAYDGDVFKSREHSERLLRSARILGFDVPYAAEEIDRAKRELVTRMGLPDCYLRALSW
                     RGSEQLGVSAKHNTIHTAIAAWYWGD"
     gene            587..1294
                     /locus_tag="VFY80_07465"
     CDS             587..1294
                     /locus_tag="VFY80_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6007785.1"
                     /translation="MNAAPAFDALDRPPHLLLVDDDDRIRELLKRYLAQAGARVSAAA
                     DAAGARKLLDSMDFDLLILDVMMPVEDGFSLAESVRKTSKVPIVLLTARGMPEDRIRG
                     LSIGADDYVPKPFEPAELALRINAILRRTVAGRGEAPEIVNFGPFAFNTGRGELSREG
                     VNVRLTEAEVAMLRILATRPGEVVSREELAKRTGAGLERSVDVQVTRLRRKMETDPRA
                     PIYLQTVRGVGYRLAAD"
     gene            1291..1602
                     /locus_tag="VFY80_07470"
     CDS             1291..1602
                     /locus_tag="VFY80_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX6007786.1"
                     /translation="MTIRKLRIKRMIRMSKQVEPLRVPAGEFQRRVGEFADIALTRPV
                     TITRNGRDRTVLISAEEYARLKRRDRQVLRVEEFSDADLDMIAASEAPDEAAAFDAES
                     E"
     gene            1692..2042
                     /locus_tag="VFY80_07475"
     CDS             1692..2042
                     /locus_tag="VFY80_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="growth inhibitor PemK"
                     /protein_id="HEX6007787.1"
                     /translation="MKDRPCAVILVAKIANEETQVTVLPITHTPPREKAHGVRLPEGT
                     CRRLGLDNEAKWIVLTEANRFVWPGPDLRGGARGDPRRVDYGFLPARFFAHVRDSFIA
                     LARKQRPTITKRSD"
BASE COUNT          390 a          739 c          672 g          372 t
ORIGIN      
        1 gtcgccccag taccaggcgg cgatcgcggt atggatggtg ttgtgcttgg ccgaaacgcc
       61 gagttgctcg gacccgcgcc aggacagagc gcgcaggtag cagtccggca ggcccatccg
      121 ggtgaccagc tcgcgcttgg cgcgatcaat ctcctcggcc gcgtaaggca cgtcgaagcc
      181 caggatgcgc gccgagcgga ggagccgctc actgtgctcg cggctcttga acacgtcccc
      241 gtcataggcg cgctcgcctt cgaacacgca cgaggcgtag tgcatggcat gcgtcagcac
      301 gtgcaccttg gcctctcgct gggggaccca ctcgccgtca aaccaaaccc acccgtcgcg
      361 gtcgtggaat ggttgtgcgg ccatgctttt cctcctggta tagggtcagc gcgcgcctgt
      421 gggacctgcg cgaatcgctc ccacgggtct gtttttggtg ataatctgtg ctgcgagaca
      481 tgcctgtcaa ccaagctcac ctgagcgtag aagcaattac ccctcaaatg tcgcatttgc
      541 gcatgttcct tgcggtgaga gcgtctctca acggggcctc ccgcacatga atgccgcccc
      601 cgccttcgac gccctcgacc ggcccccgca tctactgctt gtggatgacg acgatcggat
      661 acgcgaactg ctgaagcgct acctcgccca ggccggcgcg cgggtgtcgg ccgccgccga
      721 cgccgccggc gcgcgcaagc tgctcgacag catggacttc gacctgctca tcctcgacgt
      781 gatgatgccg gtggaagacg gcttctcgct ggccgaaagc gtgcgcaaga cctcgaaagt
      841 cccgatcgtg ctcctgaccg cgcgcggcat gcccgaagac cgcatccgcg gcctctccat
      901 cggcgccgac gattacgtgc ccaagccctt cgagcccgcc gaacttgcgc tccgcatcaa
      961 cgccatcctg cgccgcaccg tggcggggcg cggcgaagcg cccgagatcg tcaatttcgg
     1021 tcccttcgcc ttcaacaccg gccgcggcga actctcgcgc gagggggtca atgtccgcct
     1081 caccgaagcg gaagtggcca tgctgcgcat cctcgccacc cgccccggcg aagtggtcag
     1141 ccgcgaagaa ctggccaagc gcaccggcgc cggcctcgag cgttcggtcg acgtccaagt
     1201 cacgcggctc aggcgcaaga tggaaaccga tccgcgcgcg ccgatctatc tgcagaccgt
     1261 gcgaggtgtc ggctacaggc tggccgccga ttgacgatcc gtaaactacg gattaaacgt
     1321 atgatccgta tgtcaaagca agttgaaccc ttgagggtcc cagcgggcga gttccagcgc
     1381 cgggtcggcg agttcgctga cattgcgctg acgcgtccag tgaccatcac ccgcaacggc
     1441 agagaccgca ccgtcctcat ctcggcggaa gaatatgccc ggttgaagcg gcgtgaccgc
     1501 caagtgctgc gcgttgagga attctccgac gccgatctgg acatgatcgc cgcctccgaa
     1561 gcgcccgatg aagccgctgc gttcgacgcc gagtctgaat gacgtcgact ctgcctgagc
     1621 cgagggcggc gctggtcatc cgatacgcct acctgtggcg agacgagtat gagcgtgggc
     1681 aagaggaggg cgtgaaagat cgcccttgcg cagttatcct cgtcgccaaa attgcgaacg
     1741 aagagacgca agtgactgtt ctgccaatta cgcacacgcc gccgcgcgag aaagcgcacg
     1801 gcgtgagact gccggagggc acgtgccggc gcctgggcct cgataatgag gcgaagtgga
     1861 tcgtgcttac cgaagcaaat agattcgtct ggccggggcc ggacctccgc gggggcgcgc
     1921 gcggcgatcc gcggcgagtg gactacgggt tcctgccagc ccgtttcttc gcgcacgtgc
     1981 gcgacagctt catcgcgctg gctcgcaagc agcgtccgac gataacgaaa cgatccgact
     2041 gatgacggcc cagcccatct tccgccttcg cgacatcatg ccgaagggcc tctactggcg
     2101 cacgctgctc atcatcgtcg cgcccgccgc cctcatgcag ctcatcatca cgctcgtctt
     2161 cctcgacgac cac
//
LOCUS       DASURG010000424         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_280535, whole genome shotgun sequence.
ACCESSION   DASURG010000424 DASURG010000000
VERSION     DASURG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280535"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2681)
                     /gene="mfd"
                     /locus_tag="VFY80_07480"
     CDS             complement(<1..2681)
                     /gene="mfd"
                     /locus_tag="VFY80_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6007788.1"
                     /translation="MLELTLPAPGQRAQAPVLHGSSDALALAALALRARPLLVVTESA
                     WHAQRLLEEIPFFASNLRVHLFPDWETLPYDHFSPHHDLVSERLATLYQMMRGEFDVA
                     IVPVTTALCRLTPVEYLAAHTFFFKQGEKFAPEELRRQLTRAGYTHVTQVLAPGEYCY
                     RGGLIDLFPMGSALPYRIDLLDNEIESIRSFDVDTQRSIYKVPEVRLLPAREFPMDEA
                     GQTQFRRHFREKFEGDPSKSTIYRDVSKGIPSAGVEYYLPLFFDRTAVITDYLPDNTL
                     VCLQGGLENAMAEFWTETRSRYEMLRGDRANPVLPPQDMFLAADAFFGALKTFARVEL
                     GQGDDSATAPLPGLAVERRATHPLHRLQDFLGRHEGRTLILAESQGRRETMLEYFAEY
                     GLKPALCDDYAQFQASSEPVMLGVGPLHAGFVERAQRFAIITENELYAAQARTRRDRD
                     TRRATSEGMLRDLSEVKIGDPVVHEQHGIGRYLGLQEMAFEEGKSEFLILEYQGGDKL
                     YVPVAQLGVIGRYSGAQPEEAPLHKLGSGQWDKAKARAAKQVRDTAAELLALYAKRAA
                     RQGHAFGISQHDLEAFAAGFGFEETPDQAAAIEAVAADMASGKPMDRLVCGDVGFGKT
                     EVALRAAFIAVADKKQVAVLCPTTLLAEQHFQTFSDRFADWPVRIAELSRFRSAKESQ
                     GILKGLSTGDIDIVIGTHKLLGKEAKFKRLGLVIIDEEHRFGVRQKEALKRLRAEVDV
                     LTLTATPIPRTLAMSLEGIRDFSVIATAPEKRLAIKTFVAPFSEGLIREAVMRELKRG
                     GQVYFLHNEVESIERMRERLTRLLPEARIAVAHGQMRERDLGRVMRDFTAQRFNVLLC
                     STIIETGIDIPTANTIVINRADKFGLAQLHQLRGRVGR"
     gene            complement(2772..>3526)
                     /gene="amrS"
                     /locus_tag="VFY80_07485"
     CDS             complement(2772..>3526)
                     /gene="amrS"
                     /locus_tag="VFY80_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX6007789.1"
                     /translation="ASPAAIAQAAVRLGCRSVAFTYNDPVIFAEYAMDVADACRARSI
                     KTVAVTAGYIGTDARRDFYAKMDAANVDLKGFTDEFYVKLCGARLKPVLDTLVYLRHE
                     TDVWFEITTLLIPGKNDSSEELRAECRWIARELGPDVPLHFTAFHPDYKLTDIAGTPA
                     ATLTRARNIALEEGLHYVYTGNVHDPEGGTTYCPGCRAPLIVRDWYRIEEQKVTADGH
                     CPQCGHAVAGRFEPYHGQFGRRRIPLAIHTPA"
BASE COUNT          560 a         1135 c         1211 g          620 t
ORIGIN      
        1 cggccgaccc ggccgcgcag ctggtggagc tgcgcgaggc cgaacttgtc ggcgcggttg
       61 atgacgatgg tgttcgccgt cgggatgtcg atgccggtct cgatgatggt ggagcagagg
      121 agcacgttga agcgctgcgc ggtgaagtcc cgcatcacgc gcccaaggtc gcgctcgcgc
      181 atctgcccgt gtgccacggc gatccgcgcc tccggcagga ggcgggtaag ccgttccctc
      241 atcctctcga tcgactccac ctcgttgtgc aggaagtaca cctggccgcc gcgcttgagc
      301 tcgcgcatca ccgcctcgcg gatcaggccc tcggagaagg gcgcgacgaa ggtcttgatg
      361 gcgaggcgct tctccggcgc ggtggcgatg accgagaagt cgcgtatccc ttccagcgac
      421 atcgccagcg tgcgcgggat cggcgtcgcg gtgagcgtga gcacgtcgac ctccgcgcgc
      481 aggcgcttca gcgcctcctt ctggcgcacg ccgaagcgat gctcctcgtc gatgatcacg
      541 agtcccaggc gcttgaactt cgcctccttg ccgagcagct tgtgcgtgcc gatgacgatg
      601 tcgatgtctc cggtggacaa gcctttcaga attccctggg attccttcgc gcttcggaag
      661 cgcgagagct cggcgatgcg gaccggccag tcggcgaagc ggtcgctgaa ggtctggaag
      721 tgctgctcgg cgagcagcgt ggtcgggcag agcaccgcca cctgcttctt gtccgccacc
      781 gcgatgaagg cggcgcgcag cgcgacctcg gtcttgccga agccgacgtc gccgcacacg
      841 aggcggtcca tcggcttgcc cgaggccatg tcggcggcca ccgcctcgat cgccgcggcc
      901 tggtcgggcg tctcctcgaa gccgaagccc gccgcgaagg cttcgaggtc gtgctggctg
      961 atgccgaagg catgcccctg gcgcgcggcg cgcttggcgt agagcgcgag cagctccgcc
     1021 gcggtatcgc gcacctgctt cgccgcgcgg gccttcgcct tgtcccactg gccgctgccg
     1081 agcttgtgca gcggcgcctc ctcgggctgc gcgccgctgt agcggccgat gacgccgagc
     1141 tgcgccaccg gcacatagag cttgtcgccg ccctggtatt cgaggatgag gaattcgctt
     1201 ttcccttcct cgaaagccat ttcctgcagg ccgagatacc tgccgatgcc atgctgctcg
     1261 tgcaccaccg ggtcaccgat cttcacctcc gaaagatcgc gcagcatgcc ttcgctcgtg
     1321 gcgcggcggg tgtcgcggtc gcggcgcgtg cgcgcctggg cggcgtagag ttcgttctcg
     1381 gtgatgatgg cgaagcgctg cgcgcgctcg acaaaccctg cgtggagcgg accgacgccg
     1441 agcatcaccg gttcgctgga agcctggaat tgcgcgtagt cgtcgcagag cgccggcttg
     1501 aggccgtact cggcgaagta ttcgagcatc gtctcgcgcc ggccctggct ctcggcgagg
     1561 atcagcgtgc gcccctcgtg tcggccgagg aagtcttgga gacggtgcag cggatgggtt
     1621 gcgcgacgct cgacggcgag gccgggaagg ggggccgtgg cactatcgtc cccttggccc
     1681 aactcgacgc gtgcaaatgt cttgagcgcg ccgaaaaacg cgtccgcggc gagaaacata
     1741 tcctgcggcg gcagcaccgg gtttgcgcga tcgccgcgca acatctcgta acgggagcgc
     1801 gtctccgtcc agaactccgc catcgcgttt tcgagcccgc cctgcaggca gacgagcgta
     1861 ttgtcgggca ggtagtcggt gatgaccgcc gtccggtcga agaagagcgg gaggtagtac
     1921 tccacgcccg cgctcgggat gcctttgctg acatcgcggt agatcgtgct cttcgacgga
     1981 tcgccctcga acttctcacg gaagtgccgg cgaaactgcg tctgacccgc ttcgtccatc
     2041 ggaaattcgc gcgcgggcag cagccgcacc tccggtacct tgtagatgct gcgctgggta
     2101 tcgacgtcaa agctgcggat gctttcgatt tcgttgtcga ggagatcgat gcggtagggg
     2161 agcgcgctcc ccatcgggaa aagatcgatc agcccgccgc gatagcagta ctcgccgggc
     2221 gccagcacct gcgtgacgtg cgtgtagcct gcgcgcgtga gctgtctccg cagttcctcc
     2281 ggcgcgaact tctcgccctg cttgaagaag aaagtgtgcg cggcgaggta ctcgaccggt
     2341 gtcaggcggc agagcgcggt ggtcaccggc acgatcgcaa cgtcgaactc gccgcgcatc
     2401 atctggtaga gcgtggcaag ccgctcggat acgagatcgt ggtgcggtga aaagtggtcg
     2461 taggggagtg tttcccaatc cggaaagagg tgtacccgca ggttcgacgc aaagaacggg
     2521 atctcttcca gcaggcgttg agcgtgccac gcgctttcgg tcacgacgag aagcgggcgc
     2581 gcgcgcaggg cgagcgccgc cagcgcgagc gcatcgcttg atccgtgcag cacgggcgcc
     2641 tgggcgcgct gccccggcgc cggtagggtg agctccagca tcagcccgaa aaaaagcgtc
     2701 gattatacag accgctgcag ggaaagatcg cgtttgcggc cgctccgccg gctgccgcag
     2761 gcgacgagat gtcaggccgg cgtgtggatc gcgagcggga tgcggcgccg tccgaactgg
     2821 ccgtgatagg gctcgaagcg cccggcaacg gcgtgaccgc actgtgggca gtgcccgtct
     2881 gccgtcacct tctgctcctc gatgcgatac caatcgcgca cgatgagcgg cgcgcggcaa
     2941 cccgggcagt aggtggtgcc cccttcgggg tcgtggacgt tgcctgtata gacgtagtgc
     3001 aggccctctt cgagcgcgat attccgcgca cgcgtcaacg tcgcggccgg cgttccggca
     3061 atatcggtca gcttgtagtc gggatgaaac gcggtgaagt gcagcggcac gtcgggcccg
     3121 agctcgcgtg caatccagcg gcactcggcg cgcagctcct cgctcgaatc gttcttgccc
     3181 gggatgagga gcgtcgtgat ctcgaaccat acgtccgtct cgtgcctgag atagacgagt
     3241 gtgtcgagca cgggcttgag ccgcgcgccg cagagtttca cgtagaactc gtcggtaaag
     3301 cctttgagat cgacgtttgc ggcatccatc ttcgcgtaga aatcacgacg tgcgtccgtg
     3361 ccgatgtagc cggcggtcac ggccacagtt ttgatgctgc gcgcacggca ggcgtcggcc
     3421 acgtccatgg cgtactcggc gaagatcacc gggtcgttgt aagtgaacgc caccgacctg
     3481 cagcccaggc gcacggccgc ctgcgcgatc gccgcggggc tcgcct
//
LOCUS       DASURG010000425         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_483479, whole genome shotgun sequence.
ACCESSION   DASURG010000425 DASURG010000000
VERSION     DASURG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483479"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            785..1894
                     /locus_tag="VFY80_07490"
     CDS             785..1894
                     /locus_tag="VFY80_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6007790.1"
                     /translation="MAMDTVSLIKTMLTAGEGISDLIFSPGRPPQVERHGHLVAVDGI
                     ETLRADDTARIAADLIAGNDQLLRTLKEQGACDLSYSLAETSRFRVNIFKQRGSHAVV
                     MRVIPPRVPTLAELNLPTQLTELAALKNGIVLVTGPTGSGKSSTLAAIIDLINETRAD
                     HILTIEDPIEFLHTHKKGTVHQRELHTDTPTFAAALRAALRQAPKVILVGEMRDRETI
                     EIALTAAETGHLVFSTLHTIDASKTVERIVGAFEQGDQQAIRTRLAASFRYFISQKLI
                     PKKSGGRMPVLEVLKSTMRTREYIENGDTEGKSLLDAMRDGELDGMRHFDGELEKLVR
                     AGEITLETAYLYATNAGNLRIQLADVPDQSSLIIR"
BASE COUNT          393 a          665 c          662 g          345 t
ORIGIN      
        1 agaacgcgaa ggtgcatctg aaccagggca ggtcgtatgg ccgcggcggt gagggtcaca
       61 tgcggatgaa catcggcacg tcgcggcagc tggtgaagcg tgcgctggag aacattgccg
      121 aggcgctgag caagccgacg ctcacgtccg cctgacgcgc cgcgtcccga acacgacgcg
      181 gtccccggtt cgagcgactc agccccgcct tcgggcgggg ctgatcgcgc gcctagcttt
      241 cctgattccc cgaagaacga tgtcttgtcc ttcgacccgc gcacgccggg ggtgtgcccg
      301 gttctgtgtg tgtttagggc gcagagaatc gacatgcgct cgcccgaatc gcggaggaag
      361 tgaggtgtag ccccttttct gagcattgaa aaaggggctg cgctgttgac gttactgcaa
      421 atggggacag tccccatttc ccacttctgc aaatggggac tgtccccaat ttccttcagt
      481 gcccagtctt cgcagaaagt gctactcggc gcgcaacgtc tcggccggat caatccgcgc
      541 cgcccgcgcg ccggcgctcc gggagaacat ccgccgccac gggtcgtggc tgaggacgac
      601 ggccttcacg ccgggccgag acctatggcg agcgtgagga ctgcggtcga aaagaaagac
      661 ggttgccgag ggaaactgcg gcacgcgcag cggaaatcct gtgcaacggt gtcgagccgg
      721 atccaacgcc gtgcagggat tggcatctgg cctctacgag aagacgtgaa atcgaaggta
      781 tcgcatggcc atggatacgg tatcgctcat caagacaatg ctcaccgccg gcgaaggcat
      841 cagcgatctg atcttctcgc ccgggcggcc gccccaggtc gagcgccacg gtcacctcgt
      901 cgccgttgac ggcatcgaga cgctgcgcgc ggacgatacg gcgcgcattg ccgccgacct
      961 gatcgcgggc aacgaccagc tgctgaggac gctcaaagag cagggggcgt gcgatctctc
     1021 ctactcgctg gccgaaacct cacggttccg cgtcaacatc ttcaagcagc gcggcagcca
     1081 tgccgtggtg atgcgcgtca ttccgccgcg cgtgcccacg cttgcggagc tcaacctgcc
     1141 gacccagctg accgagcttg ccgcgctcaa gaacggcatc gtgctggtca ccgggcccac
     1201 cggatcgggc aagtcgtcga cgctggcggc gattatcgat ctgatcaacg aaacgcgtgc
     1261 cgatcacatc ctgacgatcg aagatccgat cgagttcctg cacacccaca agaaagggac
     1321 cgtgcatcag cgcgagctgc acaccgacac gccgacgttt gccgcggccc tgcgcgccgc
     1381 gctgcgtcag gcgccgaagg tgatcctcgt cggcgagatg cgggaccgcg agaccatcga
     1441 gatcgcgctg accgcagccg agaccggtca cctcgtcttc tccacgctgc acaccatcga
     1501 tgcgtcgaag acggtcgagc gcattgtcgg tgcgttcgag cagggcgatc agcaggcgat
     1561 ccgtacgcgg ctggcggcgt cgttccgcta tttcatttca cagaagctga tcccgaagaa
     1621 gtcaggcggg cgcatgccgg tgctcgaggt gctgaagtcc accatgcgca cccgcgagta
     1681 catcgagaac ggcgatacgg aagggaagtc gctgctcgat gccatgcgcg acggcgagct
     1741 cgatggcatg cgccacttcg acggcgagct ggaaaagctg gtgcgcgcag gcgagatcac
     1801 gctcgaaacg gcatatctct acgcgaccaa tgccggcaat ctgcggattc aactggccga
     1861 cgtcccggac caatcgtcgt tgatcatccg gtaacgcgaa cgtcgctgcc ggcgcggact
     1921 tgatatcgta cacggcatga ccgctttggc agcaacccgg ctcggcgccc ctgacgctcg
     1981 tcagcatctc gtgatgctgc acggcatcta cggtcgcggg cgcaactggc agtcgattgc
     2041 gcgggcagtc gtcgccgccc ggcca
//
LOCUS       DASURG010000426         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_994679, whole genome shotgun sequence.
ACCESSION   DASURG010000426 DASURG010000000
VERSION     DASURG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994679"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFY80_07495"
     CDS             <1..735
                     /locus_tag="VFY80_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007791.1"
                     /translation="AKATPDGHTLLVGSSGPLSVNPVVFRDIPYDSVKDFAPVSLVQI
                     VPLVVLVSPKFNVASVKELIARAKAQPGKITMASAGSGTTNHFAIELFSSLTGAKFLH
                     VPYKGSGPALGELLGGQVNSMVDQLTSSMGFIRDGKLKVIAVTAAKRVSALPNVPTLA
                     ESGVPNYEASTYLGILAPAGTSRDVVMKLNGALRKVVEASAVQERFRSLGADPGGSTP
                     EAFARMIRDELAKWRSLAKSANLQFN"
     gene            777..1637
                     /locus_tag="VFY80_07500"
     CDS             777..1637
                     /locus_tag="VFY80_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6007792.1"
                     /translation="MNESRCIVAPGVADALAARLVALEGFEAIYMTGFGTSLTRLGMP
                     DVGLLTASEMVDNASRIADASGLPLIADADTGYGNPINVRRTIRDYEKAGVAAVHVED
                     QVWPKRCGHLAGKRVIPAAEMVAKIKAACDARRDGDLAIIARTDAIAVEGLDSALERA
                     ERYREAGADMLFIEAPVGRAEVERVARHFQGVPLLYNMAASGKTPDLPAAELGRLGFR
                     LAIYPNWVILAAIPAMQHLLRELKRTGSIAHLREGVATFRQFTDIAGLPEIQQLEERY
                     GVPDDQRASL"
     gene            1639..>2815
                     /locus_tag="VFY80_07505"
     CDS             1639..>2815
                     /locus_tag="VFY80_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6007793.1"
                     /translation="MIAPRTLFEKIWSRHVVAMRGEDEALLYIDRNLVHDGSFHAFGA
                     LAKEGRSVRRPEQTFAVVDHYVPTVGRERGVAGISDPEARTMIELLQRNAEAHGIMHV
                     GIDDPRQGIVHVVGPELGLTQPGFTIACGDSHTSTHGAVGAYAFGIGASQLKQILATQ
                     CLWQKKPRTLRVTVDGRLGADVTAKDVILAIIAKIGTAGATGHVIEYAGTAVHSLSME
                     GRLTVCNMSIEAGARAGMVAPDDVTYQYLAGREYAPTEAYWESALAYWRTLPTDDGAP
                     FDAEVSLNGGELTPMVTWGTSPEEAASVAAVVPDPNQISDLNKRAHAVQALAYMRLQP
                     GMLLTDIAIDRAFFGTCTNGRIEDLRAAARVLTGRKVVVPAWVSPGSSSVKRQAEAEG
                     "
BASE COUNT          521 a          887 c          944 g          463 t
ORIGIN      
        1 gcaaaggcga cccccgacgg ccatacgctg ctggtcggtt cgagcgggcc gctttccgtc
       61 aatcccgtgg tgttcaggga tatcccctac gattccgtga aggactttgc gccggtcagt
      121 ctcgtccaga tcgtgccgct cgtcgtgctg gtcagcccaa agttcaacgt ggcgtccgtg
      181 aaggagttga tcgcccgcgc gaaggctcag ccgggcaaga tcacgatggc gtccgccgga
      241 tcgggcacga cgaatcactt tgccatcgag cttttcagct cgctcaccgg cgcgaaattc
      301 ctgcacgtgc cgtacaaggg cagcgggccc gccctcggcg agctgctcgg cggtcaggtg
      361 aacagcatgg tcgatcagct cacctcctcc atgggcttta tcagggacgg caagctcaaa
      421 gtgatcgcgg taacggcggc gaagcgcgtg agcgcgcttc ccaacgtgcc gacgctcgcg
      481 gagtcgggcg ttccaaatta cgaggcgagc acttaccttg gcatcctggc gcccgccgga
      541 acgtcgcgcg acgtcgtgat gaagctgaac ggcgcgctgc gcaaggtcgt cgaagcgtcg
      601 gcagtacagg agcgttttcg cagtctcggg gcggatccgg gcggcagcac gccggaagcg
      661 ttcgcgcgca tgattcgcga cgagctcgcg aagtggcgca gcctcgcgaa atccgccaac
      721 ctgcagttca actagcaagc acctcgatga aacccgctgc ccgccttcgc gctctgttga
      781 acgaatcccg ctgcatcgtg gccccggggg tggcagacgc gctcgcggca cgtctggtcg
      841 cgctggaagg attcgaggcg atctacatga ccggattcgg cacgagcttg acgcggctcg
      901 gcatgcccga tgtcgggctc ctcaccgcaa gcgagatggt ggacaacgcg agccgcattg
      961 cggacgcatc cgggctgccg ctcatcgcag acgcggacac cggatatggc aacccgatca
     1021 acgtgcggcg cacgatacgg gactacgaaa aggccggcgt cgcggccgtc catgtggagg
     1081 atcaggtgtg gcccaagcgc tgcggccacc tcgctggcaa acgcgtgatt cccgccgcgg
     1141 agatggtggc caagatcaag gccgcgtgcg atgcgcgccg cgatggcgac ttggccatca
     1201 tcgcgcgcac cgacgcgatc gcggtcgaag ggctggattc ggccctggag cgggcagagc
     1261 gttatcgcga ggcgggcgcc gacatgctgt ttatcgaggc tcccgtgggg cgggccgaag
     1321 tcgagcgcgt cgcgcgccat ttccagggcg tgccgctcct ctacaacatg gcggcgagcg
     1381 gcaagacgcc cgatctgccg gccgcagaac tgggtcgcct cggtttcaga ctcgccatct
     1441 accctaattg ggtcatcctc gccgcgattc cagcaatgca gcatctcctg cgagagctca
     1501 aacgaaccgg cagcatcgcc catctgcgcg agggggtggc gacgttcagg caattcaccg
     1561 acatcgcggg actgccggaa atccagcagc tggaagagcg ttatggcgtg ccggacgacc
     1621 agcgcgcgag cctatgacat gattgcaccg cgcacgcttt tcgagaagat ctggtcgcgc
     1681 cacgtcgtcg caatgcgcgg cgaggacgag gcactcctct acatcgaccg caatctggtg
     1741 cacgacggat cgtttcacgc cttcggcgcg ctcgccaagg aagggcgcag cgtgcgcagg
     1801 ccggagcaga ccttcgcggt cgtcgaccat tacgtgccga cggtgggccg ggagcgtggc
     1861 gtcgcgggca tcagcgaccc ggaagcgcgc acgatgatcg agctgctcca acgcaacgcc
     1921 gaggctcacg gtatcatgca tgtcggcatc gacgatccgc ggcaagggat cgtccatgta
     1981 gtagggcctg agttagggct cactcagcca gggttcacga ttgcctgcgg cgattcccat
     2041 acttccacac atggcgccgt gggcgcctat gcgttcggta tcggcgcctc gcagttgaag
     2101 cagatccttg ccacgcagtg cctgtggcag aaaaagccgc gcaccctccg cgtcacggtg
     2161 gacggaaggc ttggcgcaga cgtgacggca aaggatgtga tcctcgcgat catcgcaaag
     2221 atcggaacgg cgggtgcgac cggccacgtc atcgagtacg cgggcacggc ggtccactcg
     2281 ctctcaatgg aagggcgcct caccgtatgc aacatgtcga tcgaggccgg cgcgcgcgcc
     2341 gggatggtcg cgccggacga cgtcacgtat cagtaccttg cgggccgcga gtacgccccg
     2401 acggaagcct actgggaaag cgcgctcgcc tactggcgga cactgccaac cgatgacggc
     2461 gcaccgttcg atgcggaggt cagtctgaac gggggtgaac tgacgcccat ggtgacgtgg
     2521 ggcaccagtc cggaggaggc ggcatcggtg gcagcggttg tcccagatcc aaaccagatc
     2581 agtgacttga ataaacgcgc ccatgcggtg caagcacttg cttacatgag actgcagccg
     2641 gggatgctgc taacggatat tgccatcgat cgggcatttt tcggcacctg cacgaatggt
     2701 cggatcgagg atctgcgcgc ggcggcccgg gtgctcacgg gccgcaaagt cgtcgtaccc
     2761 gcgtgggtgt cgccgggatc gagcagcgtc aagcgccaag cagaggccga gggtc
//
LOCUS       DASURG010000427         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_382116, whole genome shotgun sequence.
ACCESSION   DASURG010000427 DASURG010000000
VERSION     DASURG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382116"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFY80_07510"
     CDS             complement(<1..423)
                     /locus_tag="VFY80_07510"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HEX6007794.1"
                     /translation="MGENNRALSEGAVEHDPIAQFALWYDEACAVERPLPHAVALATS
                     APDGQPSLRMVLLKGFDAHGFVFFTNYLSRKGRELEKNNRASLLFYWGTLERQVRIEG
                     RIAKVSTRESDEYFRTRPRGSQLSACASPQSEVVPDRAA"
     gene            complement(442..933)
                     /locus_tag="VFY80_07515"
     CDS             complement(442..933)
                     /locus_tag="VFY80_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX6007795.1"
                     /translation="MYRPRREVDWLAAMLFAAILSGCSVPRPGDGPREPAPAPRIPVS
                     ASDGQWELVTSTFIGAGRIPGVRRATLAFEDGRIAVFSGCNQGSASAYHVEGRLEVAA
                     LIATRRACPEPLGTFEARYFKLLQARPVYRMDGDTLMLLDGEQSARFRRLQQGATGSA
                     GKP"
     gene            complement(954..>2043)
                     /locus_tag="VFY80_07520"
     CDS             complement(954..>2043)
                     /locus_tag="VFY80_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX6007796.1"
                     /translation="IIGANLHNLKALHTRIPLGRMVGVTGVSGSGKSTLAREVLYRSL
                     VRLLADRRNGKVRAKSAAGLNGASGCRELKGWQYVGRVLEVDQTPIGKTPRSCPATYV
                     GFWDRVRQLFAEVPEARMRGYTAGRFSFNTKGGRCETCEGQGLRKIEMSFLPDVKVPC
                     ETCRGARFDPETLAVRFKGRSIGEVLAMNVDEAVEFFSAHPSIHHCLKLLQEVGLGYL
                     TLGQQSPTLSGGEAQRIKLVTELAKVRSTHAANHTLYVLDEPTVGLHMADVEKLIHVL
                     HQLVDAGNTVVVIEHNLDIIAECDWVIDLGPEGGAAGGRVVAEGTPEDLIREYGLASA
                     GAGSTSARTVSHTARVLSEFFSSRRRFQ"
BASE COUNT          339 a          696 c          641 g          367 t
ORIGIN      
        1 cgcggcgcgg tctggaacca cttcgctctg gggtgacgcg caggcggaaa gctggctgcc
       61 gcgcgggcgc gttctgaaat actcatctga ttcccgcgtc gacacttttg cgatgcggcc
      121 ttcgatgcgc acctgccgtt cgagcgttcc ccagtagaaa agaaggcttg cgcggttgtt
      181 cttttccagc tcgcggccct tgcggctcag ataattggtg aaaaacacga atccgtgcgc
      241 gtcgaagccc ttcaacagca ccatgcgcag cgacggctgg ccatccgggg ccgacgtcgc
      301 gagcgccacc gcatgcggca acggtcgctc caccgcgcac gcctcgtcgt accacaaggc
      361 aaactgcgcg atcgggtcgt gctcgacagc gccctcgctc aatgcccgat tgttctctcc
      421 catgtgcccg attctactcg cttagggttt gccggcgctt cccgtggccc cttgctgcag
      481 acggcggaac ctcgcgctct gttcgccgtc caggagcatc agcgtgtcgc cgtccatgcg
      541 atagaccggg cgggcctgca agagcttgaa ataccgcgct tcgaacgtgc cgagcggttc
      601 ggggcaggcg cgccgcgtgg cgatcagtgc ggcgacttcc aagcgtccct cgacatgata
      661 tgcggacgca ctgccctggt tgcagccgct gaacaccgct atgcgcccgt cctcgaaggc
      721 gagcgtcgcg cggcgaacgc cgggaatgcg ccctgccccg atgaacgtgg acgtgacgag
      781 ttcccattgg ccgtcggacg cggacaccgg gattcttggc gccggtgcag gctcccgcgg
      841 tccgtcgccc ggccggggca cgctgcagcc ggagagtatg gcggcgaaga gcattgcggc
      901 gagccaatcg acttcgcggc ggggacggta cattgctttc tccttcacgg ctgtcattgg
      961 aaccggcggc ggctcgagaa aaactcggaa agcacacgcg cggtatgcga cacggtccga
     1021 gcggatgtgc tgccggcgcc cgccgatgcg agcccgtatt cgcggatcag atcctcgggc
     1081 gtgccttccg ccaccacgcg accgcccgcg gcgccgcctt cggggccgag atcgatcacc
     1141 cagtcgcatt cggcgatgat gtcaagattg tgctcgatca ccaccacggt gttcccggca
     1201 tccacgagct ggtggagcac gtggatcaat ttctcgacgt ccgccatgtg cagccccacc
     1261 gtcggctcgt ccagcacata cagcgtgtga ttcgccgcgt gcgtgctgcg caccttcgca
     1321 agctccgtga cgagcttgat gcgctgcgct tcacccccgg aaagcgtcgg gctctgctgg
     1381 cccagcgtca gataaccgag ccccacctcc tgcagcagct tgaggcaatg gtgaatagac
     1441 gggtgcgcgc tgaaaaattc gaccgcttcg tcgacattca tcgccagcac ctctccgatg
     1501 gagcggcctt tgaaccggac ggccagcgtt tcgggatcga agcgcgcgcc gcggcaggtt
     1561 tcgcagggca ccttcacgtc ggggagaaag ctcatttcga tttttctcag accctgcccc
     1621 tcgcacgtct cgcagcggcc gcccttcgta ttgaaggaaa agcgcccggc cgtgtagccg
     1681 cgcatgcggg cttccgggac ttcggcgaag agctgccgga cgcggtccca gaaaccgacg
     1741 tacgtcgcgg gacaggagcg gggcgtcttg ccgatcggcg tctggtcgac ttccagcacg
     1801 cgtccgacat actgccagcc cttcagctcc cgacagccgg atgcgccatt gaggcccgcc
     1861 gcgctctttg cgcgcacctt tccgtttcgc cggtccgcaa gcaggcgcac gagactccgg
     1921 tagagcacct cgcgcgcgag cgtcgatttt cccgatcccg aaacgcccgt caccccgacc
     1981 atacgcccga gcgggatgcg ggtgtgaagc gccttcaaat tgtgcaggtt cgcgccgatg
     2041 atc
//
LOCUS       DASURG010000428         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_281017, whole genome shotgun sequence.
ACCESSION   DASURG010000428 DASURG010000000
VERSION     DASURG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281017"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1226)
                     /locus_tag="VFY80_07525"
     CDS             complement(33..1226)
                     /locus_tag="VFY80_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6007797.1"
                     /translation="MDQEQIFPRFSEPEYKRREAVVRREMEARGLDALVIAGDSGGRT
                     ANQANVYWLTNWVDVMVNYVVITRTRGPFLFVSYPLYLHTAKRAGRAETIEWGQLNPG
                     GDIAKRLIDLGHAKDRIGIVGVRNIARGSMLADHRDALIKTMPDAHFEDALDVMVNAR
                     MIKSREEIGWFERGAAFTDKVIENLARNMKVGMPEYMLSALIHEPVLREGGTVRLQFV
                     GATPMEAPEIIFPWQYPSHRKLERGDVVLTEISASWGGCSGQIQRPFAIAAKPTPLYQ
                     RLYDVAAECYRRIFEVLTPGATDADIRNAAMFVEREGFRSFDILIHGWGLQIEPPRVD
                     LPGSLIKRELTPVTFQPGMLIVIQPHIVTADSKAGVQVGNLVVIEERGARSLHKYPME
                     FIRVE"
     gene            1270..2229
                     /locus_tag="VFY80_07530"
     CDS             1270..2229
                     /locus_tag="VFY80_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007798.1"
                     /translation="MLRLIAIAGAFFSVVAHAQTAYPTKPVRMIVPYAPGGPIDVWGR
                     ILAQELTKAFGQSVVIDNRPGANGIVGTDILAKSPPDGYTFLYQTGSHVANVSMYRKL
                     PYDSVRDFQPITQMATTHGMVLVVHPSVPARSLKEFIALVKARPGKLNFASAGPGNAT
                     HLAAEMMKAAAGLDIVNVSYKGGGPALNDVIAGQVEMMMVSVAQGTPFVRSGKVRALA
                     ITAARRAPPLADVPTFQESGFPDFEFAGWHGLWFPANVPRDRLERMYGEVKKIMFAPE
                     MKLRLAELGLIPLATTPAEFANFIDREIAFYAKLIKAAKIEPQ"
BASE COUNT          448 a          760 c          685 g          454 t
ORIGIN      
        1 agcgggacgg ccgccgcaac aatcagaagc gatcactcga ctcgtatgaa ctccatcgga
       61 tacttgtgca acgaccgcgc gcctctttcc tcgatcacga caagattgcc cacctgcacg
      121 ccggccttcg aatcagcggt aacgatgtgc ggctgaatca cgatgagcat tcctggctgg
      181 aacgttaccg gcgtcagctc acgcttgatc agcgagcccg gcaaatcgac gcgcggtggc
      241 tcgatctgca ggccccagcc gtgaatgagg atgtcgaatg agcgaaatcc ttccctctcc
      301 acgaacattg ccgcattgcg tatatcggcg tctgtcgcgc cgggtgtgag cacttcgaag
      361 atgcgccgat agcactccgc ggcgacgtca tacagccgct gatagagcgg cgtcggcttc
      421 gccgcgatcg cgaacggcct ctggatctgc cctgagcagc cgccccagct cgcgctgatc
      481 tcggtgagca cgacatcgcc gcgttccagt ttccggtgcg acggatactg ccacgggaag
      541 atgatctcag gcgcttccat cggcgttgcg ccgacgaact ggaggcgcac cgtgccgcct
      601 tcgcgcagca ccggctcgtg tatgagcgcc gacagcatgt actcgggcat gccgaccttc
      661 atgttccgcg cgaggttttc aatcaccttg tcggtgaacg ccgccccgcg ttcgaaccat
      721 ccgatctcct cgcggctctt gatcatgcgc gcgttcacca tcacgtcgag cgcgtcttca
      781 aagtgcgcgt ccggcatcgt cttgataagc gcgtcgcgat ggtcggcgag catcgagccg
      841 cgcgcgatgt tgcgtacgcc cacgatgccg atgcgatcct ttgcatgccc gaggtcgatc
      901 agccgcttcg caatatcgcc gccgggattg agctgacccc actcgatcgt ttctgcgcgc
      961 ccggcacgtt tcgccgtgtg gagataaagc ggatagctta cgaagagaaa tgggccccgc
     1021 gttcgcgtga tgacgacgta attcaccatc acgtcgaccc agttcgtgag ccagtacacg
     1081 ttcgcctggt tcgccgtgcg cccgcccgaa tcacccgcga ttaccagcgc gtcgagcccc
     1141 cgcgcttcca tctcccgtcg caccaccgct tcacggcgct tgtattcggg ttcggaaaat
     1201 cgcggaaaga tctgctcctg gtccatgcgc ttcctctgga agggcccaca ctatacaata
     1261 actcaccgta tgctacgtct gatcgcaatc gctggcgcat tcttcagtgt cgtagcgcac
     1321 gcgcaaactg cttatccgac caagcccgtc cgcatgatcg tgccgtacgc acccggcggg
     1381 ccgatcgacg tgtgggggcg catcctcgcg caggaactca caaaagcatt cggccaatcg
     1441 gtcgtgatcg acaaccgtcc gggtgcgaac ggcatcgtcg gcaccgacat cctcgcgaag
     1501 agtccgcctg acggatacac cttcctgtat cagaccggca gccatgtcgc aaacgtgagc
     1561 atgtaccgaa agctcccgta cgactcggtg cgcgatttcc agcccatcac gcagatggca
     1621 accacgcacg gtatggtgct cgtcgtccat ccatcggtgc cggcgagaag tctgaaggaa
     1681 ttcattgcgc ttgtgaaagc gcgccccggt aagctcaatt tcgcgtccgc cggcccgggc
     1741 aacgcgacgc accttgccgc cgagatgatg aaggccgctg ctggtctcga tatcgtgaac
     1801 gtaagctaca aaggcggcgg ccccgcgctc aacgacgtca tcgcgggtca agtcgagatg
     1861 atgatggtaa gcgtcgccca gggtacgccc ttcgtgagat cgggcaaggt gcgtgccctc
     1921 gcgatcacgg ccgcgaggcg cgcgccgccg ctggcggacg tgccaacgtt tcaggaatct
     1981 ggctttcccg atttcgagtt cgcaggatgg catggcttgt ggtttcccgc gaatgtgccg
     2041 cgcgatcggc tcgagcgcat gtatggggaa gtgaagaaga tcatgttcgc gcccgagatg
     2101 aagctacggc tcgccgaact cgggctcatt ccgctcgcga cgaccccagc ggaattcgcg
     2161 aatttcatcg accgcgagat cgcgttctac gcgaagctca tcaaggccgc gaagatcgag
     2221 ccgcagtaac gtctcgcccg ccggagcgcg tccggcacga tggccgtaaa cgggcgaaga
     2281 ctttatcgac cgccattttg gaattacagt cgattggttt ccggagttca tccggaacgc
     2341 tactgca
//
LOCUS       DASURG010000429         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1199310, whole genome shotgun sequence.
ACCESSION   DASURG010000429 DASURG010000000
VERSION     DASURG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199310"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /gene="nadA"
                     /locus_tag="VFY80_07535"
     CDS             <1..1015
                     /gene="nadA"
                     /locus_tag="VFY80_07535"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX6007799.1"
                     /translation="KLGKRAVILGHHYQRADVYKHADLTGDSLKLSKLAAQTDAHYIV
                     FCGVHFMAEVADILSKPEQIAILPDLNAGCSMADMANLSKVERAWRELKQVLDPDEHV
                     TPVTYINSAADLKAFCGEHGGIVCTSTNAREILEWSFARREKVLFFPDQHLGRWTGYL
                     MDIPLSEMLVWDPDEEMGGLTPQQIKMAKILLWKGHCSVHQMFQAQHILRWRQQHPGG
                     MVVSHPECNFEVCKLSDYVGSTEYIIKTVAASAPGTRWLVGTELNLVNRIAEEFKPQG
                     KIVQFMAPTVCMCSTMQRIDPQHLAWALENLVAGKVVNQIKVPEHEAKLARIALDRML
                     AVS"
     gene            1106..1909
                     /locus_tag="VFY80_07540"
     CDS             1106..1909
                     /locus_tag="VFY80_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6007800.1"
                     /translation="MSFISAPTARPIPTSVKDTLRVATYNIHKGLSFFKRRMVIHELR
                     ERLRALDVDLVFLQEVLGNHERYARRHHDWPASPQYEFLADSVWQQYAYGRNAVYDFG
                     HHGNAVLSRYPIARWDNQDVSTHRFERRGLLHCEIGIPNWDEPLHCICVHLGLFARGR
                     SRQIEWLRQRIEKLVPREAPLIVAGDFNDWHWRHKATHELAQPLDMHEVFEVVHGAPA
                     RTFPAMLPVFRLDRIYVRGFRIGYAHAHHGNIWARISDHAVLTTVLERI"
     gene            1906..3072
                     /gene="clsB"
                     /locus_tag="VFY80_07545"
     CDS             1906..3072
                     /gene="clsB"
                     /locus_tag="VFY80_07545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEX6007801.1"
                     /translation="MSDFVDGNRITLLTRGLDYFPQLEAACDAAQHEIHIEAYIYDDD
                     EAGQRIGAALKRAARRGVAVHVLIDGFGSKRLPRAFIADLADAGVHVLVYRPERTPVF
                     MARRRLRRMHRKLAVIDARVAFVGGINIIDDMHTPGHTPPRFDYAVRVEGPLLGKIHP
                     AVKRLWALVEFMQLGRDWHDVEKLQPDATPRGTQQAAFVVRDNLRHRRDIEEAYLEAI
                     AAARKEIIIANAYFFPGLSFRRALMDAAARGVSVTLLLQGRVEYVLLHYASRALYGAF
                     LDAGIRISEYHKSFLHAKVAVIDGAWATVGSSNIDPFSLLLAREANVVVRDRTFAGAL
                     RESLVAAMAEGARDVKRERWRQQPLPVRVVTWIAYGIARLLTGLFAYGRAREFT"
     gene            3242..>4092
                     /locus_tag="VFY80_07550"
     CDS             3242..>4092
                     /locus_tag="VFY80_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6007802.1"
                     /translation="MNPHSSTLPRAAARPHAGRRTREWRVVPMLLPYLWEFKGRVVVA
                     LLFLTIAKLANVGVPLILKEVVDALDPSRAVLVLPLALLVAYGVLRLSTTLFAELRDV
                     VFVRVTQRAIRRIALTVFGHLHTLSLRFHLERQTGGVSRDIERGTRGISTLLSYMLFS
                     IIPVILEFTLVAVVLFARFDWRFVAVTFVAIALYILFTVAITEWRMEIRRRANELDSK
                     ANTRAIDSLLNYETVKYFGNEEFEARRYDENLRKYEGAAVKSESSLGLLNIGQSTIIA
                     GAVTALM"
BASE COUNT          689 a         1387 c         1283 g          733 t
ORIGIN      
        1 gaaactcggc aagcgggccg tcatcctcgg gcatcactat caacgcgccg acgtctacaa
       61 gcacgctgat ctcaccggag actcgctcaa gctttccaag ctcgcggcgc agacggacgc
      121 gcattacatc gttttctgcg gcgtccattt catggcggag gtcgcggaca tcctctcgaa
      181 gcccgagcag atcgcgattc tgcctgacct caacgccggc tgctccatgg ctgacatggc
      241 gaatctctcg aaggtcgagc gcgcatggcg ggaactgaag caggtgctcg atccggacga
      301 gcatgtgacg ccggtgactt acatcaacag cgcagccgac ctcaaggcct tctgcggcga
      361 gcacggcggc atcgtctgca cctccaccaa cgcgcgcgaa atcctggagt ggagcttcgc
      421 gcgccgcgag aaggtgctgt tctttcctga ccagcacctc ggccgctgga cgggttatct
      481 catggacatc ccgctctcgg agatgctggt gtgggatccg gacgaggaaa tgggaggctt
      541 gaccccccag cagatcaaga tggcgaagat cctgctgtgg aaagggcact gctcggtgca
      601 ccagatgttc caggcgcagc acatcctgcg ctggcgccag cagcatccgg gcggcatggt
      661 ggtgagccac ccggaatgca acttcgaggt gtgcaagctc tcggactatg tcggctccac
      721 cgagtacatc atcaagacgg tcgcggcgag cgctccgggc acccgctggc tcgtgggcac
      781 ggagttgaac ctcgtcaacc gcattgccga agagttcaag ccgcagggca agatcgtgca
      841 gttcatggcg ccgacagtgt gcatgtgctc gacgatgcag cgcatcgacc cgcagcacct
      901 ggcgtgggcg ctcgagaatc tggtcgccgg taaggtcgtc aatcagatca aagtaccgga
      961 gcacgaagcg aagcttgcgc gcatcgcgct cgaccgcatg cttgcggtct cctgaagccg
     1021 gtcggtccca cgctgcgcga ctgtgccttg cgccgcccgc cgggcggcat gtgtctcggc
     1081 gcgcgcttcg cgactctccc gtagaatgag cttcatttcc gcgcccaccg cgcggccgat
     1141 cccgacctcc gtgaaagaca cgctgcgcgt tgcgacctac aacatccaca agggcttgtc
     1201 gttcttcaag cgccggatgg tgatccacga gctgcgcgag cgcctgcggg cgctcgacgt
     1261 ggatctcgtg ttcctgcagg aagtgctcgg caaccacgag cgctacgcgc ggcgccatca
     1321 cgactggccg gcgtctcccc agtatgaatt cctcgccgac tcggtgtggc agcagtacgc
     1381 ctacgggcgc aacgccgtgt acgatttcgg gcaccacggc aacgcggtgc tgtcacgcta
     1441 tccgatcgcg cgctgggaca accaggacgt ctccacccat cgcttcgagc ggcggggact
     1501 cctccactgc gaaatcggca tcccgaactg ggacgaaccg ctgcactgca tctgcgtgca
     1561 cctgggactc ttcgcgcgcg ggcgcagccg ccagatcgaa tggctgcgcc agcgcatcga
     1621 gaagctcgtg cctcgcgagg cgccgctcat cgtggcgggt gacttcaacg actggcactg
     1681 gcgacacaag gcgacccacg agctcgcgca gccgctcgac atgcacgaag tgttcgaggt
     1741 ggtgcacggg gccccggcgc gcaccttccc cgccatgctg ccggtctttc gcctcgaccg
     1801 catctacgtg cgcggctttc gcatcggcta cgcgcacgcg caccacggca acatttgggc
     1861 gcgcatatcg gatcacgccg tgctgacgac cgtcctcgaa aggatatgag cgatttcgtt
     1921 gacggcaacc gcatcacgct cctcacccgc gggctcgact actttcccca gctcgaggcc
     1981 gcgtgcgatg ccgcgcagca cgagatccac atcgaagcct acatctacga cgacgacgaa
     2041 gcgggtcagc gcatcggcgc ggccctgaag cgcgccgcac gccgcggcgt tgcggtgcac
     2101 gtgctgatcg acggcttcgg ctccaagcgg ctgccgcgcg ccttcatcgc ggatctcgcc
     2161 gatgccggtg ttcacgtgct ggtgtaccgt cccgagcgca cgccggtctt catggcgcgg
     2221 cggcggctgc ggcgcatgca ccgcaagctc gcggtgatcg atgcccgtgt cgctttcgtg
     2281 ggtggcatca acatcatcga cgacatgcat acgccgggtc acacgccgcc ccgcttcgat
     2341 tacgcggtgc gcgtggaagg gcctttgctg ggaaagatcc atcccgcggt gaagcggctg
     2401 tgggcgctcg tcgaattcat gcagctcgga cgcgactggc acgacgtcga aaagcttcag
     2461 ccggacgcca cgccgcgcgg aacgcaacag gccgccttcg tcgtgcggga caacctgagg
     2521 catcgccgcg acatcgagga ggcgtatctg gaagcgatcg ccgccgcgcg caaggaaatc
     2581 atcatcgcga acgcctactt cttccctgga ttgagcttcc gccgcgcgct gatggatgca
     2641 gcggcgcgcg gcgtgagcgt cacgctcttg ctgcaggggc gggtcgaata tgtgctcctg
     2701 cactacgcct cgcgggcgct ctacggcgcg tttctcgacg ccggcatccg catttccgaa
     2761 tatcacaaga gtttcctgca tgcgaaggtc gcggtgatcg acggcgcatg ggcgacggtc
     2821 ggctcgtcca acatcgatcc cttcagcctt ctgctcgcac gcgaggcgaa cgttgtcgtg
     2881 cgcgatcgta ccttcgcggg cgcgctgcgc gagagcctcg tcgccgcgat ggccgaaggc
     2941 gcgcgcgacg tgaagcgcga gcgctggcgt cagcagccgc tgcccgtgcg cgtggtgacg
     3001 tggattgcgt acggcatcgc gcggctgctc actggcctgt ttgcctacgg acgtgcgcgc
     3061 gagttcacat gacggctcac cggcccggac gcatccggca gcggcgagaa gcgagccgcc
     3121 ctttaccgga tgtggtttgc ccgcatgacg actgtttggc acgaggcgcc ggtgtcgtga
     3181 aaagcctcct gttgttcacg gcgcgctgca cttagaataa ggctgcgtcg tcgcttccct
     3241 catgaatccg cactcctcca cactcccgcg cgccgctgcg cggccacacg ccgggcgccg
     3301 tacccgcgaa tggcgcgtcg tgccgatgct gttgccctac ctgtgggagt tcaaggggcg
     3361 cgtagtcgtc gcgctcctct ttctcaccat cgcgaagctc gccaacgtcg gcgttccact
     3421 gattctgaag gaagtggtcg atgcgctcga tccgagccgc gcggtgctgg tgctgccgct
     3481 cgcgctgctc gtcgcctacg gcgtgctgcg gctgtctacg acgctcttcg ccgaactgcg
     3541 cgacgtcgta ttcgtgcggg tcacccagcg ggcaatccgc cgcatcgcgc tcaccgtgtt
     3601 cggccacctg cacacgcttt cgctgcgctt ccacctggag cgccagacgg ggggcgtttc
     3661 gcgcgacatc gagcgcggca cgcgcggcat ctcgacgctg ctctcctaca tgctgttctc
     3721 catcatcccg gtgatcctcg agttcacact ggtcgcggtg gtgctcttcg cgcgcttcga
     3781 ctggcgcttc gtcgccgtca ccttcgtcgc catcgcgctc tacatcctct tcaccgtggc
     3841 gatcaccgag tggcgcatgg agatacgccg ccgcgcgaac gagctcgatt cgaaagcgaa
     3901 tacccgcgcg atcgacagcc tcctcaatta cgagacggtc aagtacttcg gcaacgagga
     3961 attcgaggcg cgccgctacg acgagaacct gcgcaaatac gagggcgcag ccgtcaagag
     4021 cgaatcctcc ctgggcctgc tcaacatcgg ccagagcacc atcatcgccg gtgcggtcac
     4081 cgcgctcatg ta
//
LOCUS       DASURG010000430         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1096934, whole genome shotgun sequence.
ACCESSION   DASURG010000430 DASURG010000000
VERSION     DASURG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096934"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1361)
                     /locus_tag="VFY80_07555"
     CDS             complement(72..1361)
                     /locus_tag="VFY80_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX6007803.1"
                     /translation="MSAENTLSPREFLLGSFRAALDAADPLKLVSLHLPTPPRGRTLV
                     VGAGKAAAAMALAVEQHWPANAPLDGLVITRYQHGLLTNRVTVIEAGHPVPDERGERA
                     AQEIFRRVRALGKDDLLLVLVSGGGSSLLSLPADGITIEDLRATTRELLRCGAPIQDM
                     NTVRKHLSSILGGRLAAASRAPVRALLISDVTGDDPTHIGSGPCAPDPSTYADALEIL
                     ERYEVQAPRAVLDRLTRGASGEVAETPKPGDALFRRVENRVIATAQHSLDGAANYFAA
                     HDVRAAILGDSVTGEARETAKVYGALARQVRAHGTPWAAPVALISGGECTVTVRGRGR
                     GGRCTEFLLSLAVNLAGAERIHALACDTDGIDGSEDNAGAILAPDTLARAVSLELRAR
                     RMLDDNDSYGFFSALGDLVVTGPTRTNVNDYRVILIS"
     gene            complement(1523..>2318)
                     /locus_tag="VFY80_07560"
     CDS             complement(1523..>2318)
                     /locus_tag="VFY80_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6007804.1"
                     /translation="GMEFFDKVVNVDQSPIGRTPRSNPATYTGLFTPIRDLFAGVPEA
                     RARGYGAGRFSFNVKGGRCEACQGDGVLKVEMHFLPDIYVPCDVCHGKRYNRETLEIE
                     YKGKSIHEVLGMTVEQATEFFSAVPVIARKLQTLLDVGLGYITLGQSATTLSGGEAQR
                     VKLSLELSKRDTGRTLYILDEPTTGLHFHDIDLLLRVLQRLRDHGNTVVVIEHNLDVI
                     KTADWIIDMGPEGGDGGGQVIAVGTPEEVAKDKGSHTGRYLKQVLS"
BASE COUNT          377 a          755 c          772 g          414 t
ORIGIN      
        1 aggagggagc gaaaaacaga ggagcgcaag gagcttgcgg atacgcccgg tcacgttgcg
       61 cctcgcatgg attacgagat gagtatgacg cggtagtcgt taacgttggt gcgggtgggc
      121 ccggtgacga cgagatcccc gagcgcgctg aagaagccgt agctgtcatt gtcgtcgagc
      181 atgcgtcgcg cgcggagctc gagcgacacg gcgcgcgcga gcgtatcggg agcgaggatc
      241 gcgccggcat tgtcctctga gccgtcgatg ccgtcggtgt cgcaggcgag cgcgtgtata
      301 cgctccgcgc ctgcgagatt cacggcgagc gaaagcagaa actcggtgca gcgcccgccg
      361 cggcccctgc ctcgaaccgt caccgtgcat tcgcctccgg agatgagcgc gacgggtgct
      421 gcccacggcg ttccgtgcgc tcgcacctgc cgtgcgagtg cgccatagac ctttgctgtt
      481 tcacgcgctt ctcctgtcac cgaatcgccg agtatcgccg cgcggacgtc gtgcgccgcg
      541 aaataattcg cggccccgtc cagcgagtgc tgcgctgtgg caatcacgcg attctcgacg
      601 cgtcggaaca gggcgtcgcc gggcttcggc gtttcagcca cctcgccgct cgcgccccgt
      661 gtcaggcggt cgagcacggc gcgcggcgct tgcacctcgt agcgctccag tatctccagc
      721 gcatctgcat aggtggaggg atcgggcgcg cacggtccgg agccgatgtg ggtcggatca
      781 tcgccggtta catctgaaat cagcagtgcg cgcaccgggg ccctgctcgc cgctgcgagc
      841 cggccaccga gtatcgagga gaggtgcttg cgtaccgtat tcatgtcctg gatcggcgca
      901 ccacagcgca gcagctcgcg cgtggtcgcc ctgagatcct cgatggtgat gccgtccgca
      961 ggaagcgaga gcaggctcga gccgccgccg gagacgagga cgagcagcag atcgtccttg
     1021 ccgagcgcgc gcacgcggcg aaaaatttcc tgcgcagcgc gctcaccgcg ctcgtccggc
     1081 accgggtggc cggcttcgat cacggtcacg cgattcgtga gcagcccgtg ctggtagcgt
     1141 gtgatcacga ggccgtcgag cggcgcgttc gccggccagt gctgctcgac cgcaagcgcc
     1201 atggccgcgg ccgctttgcc cgcgccgacg accagcgtgc ggccgcgcgg aggcgtgggc
     1261 aggtggagcg aaacgagctt gagcggatca gcagcgtcca gcgccgcgcg aaagctgccg
     1321 agcaggaact cgcgcggtga cagggtgttt tcagccgaca ccgggtgcct ctaccggcgt
     1381 ttctccgtca ttcccgcgaa cgccggaatc caggccagtg tccctggatg ccggtttgca
     1441 ccggcatgac gattcgaaac tggtgttcct ggatgcggat tttcaccggc atgacgagta
     1501 aggctgttca tcgattccag cgtcagctga gcacctgctt gagatagcgc ccggtatgac
     1561 tgcccttgtc cttcgctacc tcttccggcg tcccgactgc gatcacttgg ccgccgccat
     1621 ccccgccctc cggtcccata tcgatgatcc agtccgcggt cttgatgacg tcgagattat
     1681 gctcgatcac gactacggta ttgccgtggt cgcgcagccg ctgcagcacg cggagcagca
     1741 ggtcgatgtc gtggaagtgc agccccgtcg tcggctcgtc caatatatat agtgtacggc
     1801 ccgtatcgcg cttggagagc tcgagcgaga gctttacgcg ctgcgcctcg ccgccggaga
     1861 gcgtggtcgc gctctggccg agcgtgatgt agcccaggcc cacgtcgagc agcgtctgga
     1921 gcttgcgcgc gatgacgggc accgcgctga agaattccgt cgcctgctcg actgtcattc
     1981 ccagcacctc atggatgctc ttgcccttgt actcgatttc cagcgtctca cggttgtagc
     2041 gcttgccgtg gcagacatcg cacggcacgt agatgtcggg cagaaagtgc atctccacct
     2101 tcagcacgcc gtcgccctga cacgcctcgc agcgcccgcc cttcacgttg aaggagaagc
     2161 ggcccgcgcc gtagccccgg gcgcgcgctt ccggaacgcc cgcgaagagg tcgcgaatcg
     2221 gcgtgaaaag accggtgtag gtggcgggat tcgagcgcgg cgtgcgcccg atggggctct
     2281 ggtcgacgtt caccaccttg tcgaagaatt ccattccg
//
LOCUS       DASURG010000431         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_586674, whole genome shotgun sequence.
ACCESSION   DASURG010000431 DASURG010000000
VERSION     DASURG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586674"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>2098
                     /locus_tag="VFY80_07565"
     CDS             32..>2098
                     /locus_tag="VFY80_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6007805.1"
                     /translation="MRTDLERFFNPRAIAIVGASQDLNTISGQPLKFLKSHGYTGALY
                     PVNPRYAEVAGVKCHARLADLPETPDLALILVNAARVPDVLRQCGEKGVPYVIIFSSG
                     FAETGGTGAALQSELTAIAREFDIGVIGPNCQGMINIAERVYAGFGSVFNSDYEPGAV
                     SMVSQSGGFGFSVMNLSSKDGGLAFRQMVTTGNEIGISTLDLIEYFVRDPQTEIIAAY
                     IEGLKDARRLISVGRTALAAGKPVLIWKVGNTEHGQRAAASHTANLGGEMALYRAAFR
                     QAGIIQVEDIQDVVDYGRAFRSGRLPRGNRLAIITISGGAGILMTDECIGRGMALAQL
                     APETLARLREIVPSFGSLLNPVDVTAAIFNDLSLINRTLQAIVEDPNVDCIAMINASL
                     QGEIAARIAAEIVAVSGRTEKPVLLAWSARDVAAADAYAQLDAARIPHYKSPVRCGRA
                     AAVLSRYADARRRLERERLEAPLALHSATARSMLAGRPADLTEHAAKRLLAEYGIGVT
                     REALAVTQTEAVAAAKRIGYPVALKVQSPDIPHKTEARAVRLRIVSEEQLLSAYDEVL
                     RNAKNYKPDAAIEGVLVQEMLEGGVEAIAGIVNDTLFGPAVMFGLGGVFAEVLKDVVF
                     RLAPITASTAREMIAETKGCAVLEGLRGRPPADLDALADTLVRLSALAMDLEQELAEL
                     DMNPLVV"
BASE COUNT          374 a          674 c          680 g          370 t
ORIGIN      
        1 gtgcatgcgc ccgcgtcgcc gtcgcacaca tatgcgcacc gatctcgaac gtttcttcaa
       61 tccgcgcgcg atcgcgatcg tcggcgcatc ccaggacttg aacaccatca gcgggcagcc
      121 gctcaagttt ttgaagtcgc acggctatac gggcgcgctc tatccggtga atccgcgata
      181 cgccgaagtc gccggcgtga aatgccatgc caggctcgcc gaccttccgg aaacgccgga
      241 tcttgcgctc attctcgtca atgccgcccg cgttcccgac gtgctgcgtc agtgcggcga
      301 gaaaggcgtg ccgtacgtca tcattttcag ttccgggttc gcggagaccg gcggaacagg
      361 tgctgcgctc caaagcgagc tgaccgcgat cgcacgcgag ttcgacatcg gcgtcatcgg
      421 acccaactgc cagggaatga tcaacatcgc cgagcgcgtg tacgcgggct tcgggtcggt
      481 gttcaactcc gattacgaac caggcgccgt cagcatggtg tcccagagcg gaggattcgg
      541 cttttcggtg atgaacctct cctcaaagga cggtggcctt gcgtttcgcc agatggtgac
      601 caccggaaac gagatcggca tttccacgct cgacttgatc gaatatttcg tccgcgatcc
      661 gcagacggag atcatcgccg cctatatcga aggcttgaaa gacgcgcgcc gtcttatctc
      721 tgtcggcagg acggcgctcg cggccggcaa gccggtgctg atctggaaag tgggaaatac
      781 cgagcatgga cagcgtgccg cggcgtccca caccgccaac ctgggcggag agatggcgct
      841 ctaccgcgcg gcgttccggc aggcgggcat catccaggtc gaggacattc aggacgtggt
      901 cgattacggg cgcgctttcc gcagcggtcg acttccgcgc ggcaaccgcc tcgcgatcat
      961 cacgatttcc ggcggcgccg gcatcctcat gaccgacgag tgcatcggtc gcgggatggc
     1021 gctcgcgcag ctcgcaccgg agacactggc gaggctccgc gaaatcgtgc cgagctttgg
     1081 ctctctgctc aatccggtcg acgtcacggc cgcgattttc aatgacctct cgctcatcaa
     1141 ccgcacgctg caggcgatcg tcgaggaccc caacgtcgac tgcatagcga tgatcaacgc
     1201 ttcgctccag ggcgaaatcg cagcgcgcat cgccgctgaa atcgtcgcgg tgtccgggcg
     1261 caccgagaag cccgtgctgc tcgcatggag cgcacgcgac gtcgcggcag cggacgcgta
     1321 cgcgcagctc gatgccgccc gaatcccgca ttacaagtcg ccggtgcgct gcgggcgtgc
     1381 ggcggcggtc ctgtcgcggt atgccgacgc ccggcggcgg ctcgagcgtg agcgcctgga
     1441 ggcgccgctt gccttgcatt ccgcgacggc ccgttcaatg ctcgcgggca ggcccgccga
     1501 tctcacggag cacgcggcaa agcggctgct ggcggaatac gggatcggtg ttacgcgaga
     1561 agcgctcgcc gtgacgcaaa ctgaagccgt cgctgcggca aaacggatcg gctatccggt
     1621 ggcgctcaag gtgcagtcgc ccgacattcc ccacaagacc gaagcgcgcg ccgtgcggct
     1681 gcgtattgtg agcgaggagc aactcctttc cgcgtacgac gaggtactgc gcaacgcgaa
     1741 gaactacaaa ccggatgctg ccatcgaagg cgtgctcgtg caggaaatgt tggagggcgg
     1801 agtcgaagcg atcgcaggca tcgtcaacga tacgctcttc ggtcccgcgg tcatgttcgg
     1861 gctcggcggc gtctttgcgg aagtgctgaa ggacgtggtg ttccggctgg cgcccatcac
     1921 cgcgtcgacc gcgcgcgaaa tgatcgctga aacgaaaggc tgtgcggtgc tcgaaggctt
     1981 gcgcggcagg ccgccggcgg atctcgatgc gctcgccgat acgcttgtcc ggctctccgc
     2041 gctcgcgatg gatctcgagc aggagctcgc tgagctcgac atgaatcccc tggtcgtg
//
LOCUS       DASURG010000432         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1507022, whole genome shotgun sequence.
ACCESSION   DASURG010000432 DASURG010000000
VERSION     DASURG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507022"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VFY80_07570"
     CDS             complement(<1..1864)
                     /locus_tag="VFY80_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX6007806.1"
                     /translation="MTPHRAEIAIVGMAGRFPGAPDVETLWGNIANRVESVVRFTAED
                     LAGCGVDPALLSHPDYVPAGAPLSDAECFDAAFFGVNPAEARLMDPQHRLLLECAWAA
                     LEDAGIDPAAFEGRIGVFAGVARNTYLAHLLATRRDVLEAAGEYQAMIAGEKDFPATR
                     VAYKLNLRGPAVNVQTACSTSGVALHLACQSLRAGDCDVALVGGGRILVPQRAGYRYE
                     QGAILSPDGHCRAFDAGARGTIRGSGVGVIVLRRLEDAQRECDNVRALVKGSAINNDG
                     SAKVGFTAPSLTGQAAAISDALACAGVSADTIGYVEAHGTGTAIGDPIEIAALTRAFR
                     RTTRRNGYCAVSSVKANIGHLDAGAAVAGIIKAVHALEHRQIPPSPNFERPNPEIDFK
                     ASPFYVPAALSEWQSDGPRRAGVSSFGLGGTNAHIVLEEAPPAEPTSSARKRHLLLVS
                     ARTRAQLDAAAQRLGQRLASADNINMADVAYTLQVGRRGFAYRRALVCRDRHDAVRLL
                     EAHDPKRVIDGNAERPVDRVAFLFPGQGAQYVNMARAVYESEPQFRADIDRCAEVLKP
                     HLNRDLRAILFTDAQAVQAAEEEIRQTAITQPALFAVEYALARLWLSWGFEPAMM"
     gene            complement(1864..>2671)
                     /locus_tag="VFY80_07575"
     CDS             complement(1864..>2671)
                     /locus_tag="VFY80_07575"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6007807.1"
                     /translation="LGFETLKERREAYQGAHALWLEGGIRALMEVHQCSDEEAKRIMA
                     ELEQQGLTTRAFFRYLQDAIANRMLVDKSPSYAMDPNALARAERDFADPLYIHLVRHP
                     YAMVTSFESYHMEQVLYLGAHRFSPRQLGELVWLLSNRNILDFLANIPQRRQYAIRYE
                     ELVTRPHLVMQEMCARLGIPFDEELLRPYDNLERKMTDGLKPDSTPMGDTHLFQYQSI
                     SPRAAARWRGVEQDNFLSEATWQLAELLGYDRPAPALAASGAPAAGEMGA"
BASE COUNT          434 a          858 c          922 g          457 t
ORIGIN      
        1 tcatcatcgc gggctcgaag ccccacgaga gccacaggcg cgcgagcgcg tattcgacgg
       61 cgaagagcgc tggctgcgtg atggctgtct gccggatttc ctcttccgcg gcttggacgg
      121 cctgcgcatc tgtgaagaga atggcgcgga ggtcccggtt gagatgaggc ttcaacacct
      181 cggcgcagcg atcgatatcc gcgcggaatt gcggctctga ttcgtacacc gcgcgcgcca
      241 tgttgacgta ctgggcgcct tgaccgggaa agagaaacgc gacgcggtcc accggccgct
      301 cggcgttgcc gtcgataacg cgcttggggt cgtgcgcctc cagcagccgc acggcatcgt
      361 gccgatcccg gcacacgagc gcgcgacggt atgcgaagcc gcgacgcccg acctgcaggg
      421 tatacgcgac atcggccatg tttatattgt cagcgctggc cagacgctgc ccgaggcgct
      481 gggccgccgc atcgagttgc gcgcgagtgc gcgcggagac gagcagcagg tgacgcttgc
      541 gtgcgctgct cgtgggctcg gctggcggcg cttcttcgag cacgatgtgc gcgttcgttc
      601 cgccgaggcc gaaggaactc acgccggcgc gccgcggacc gtcgctttgc cattcggaca
      661 atgcggccgg cacgtagaac gggctcgctt tgaagtcgat ctcggggttg gggcgctcga
      721 agttggggct cggggggatc tgccggtgct cgagcgcgtg cacggccttg atgatccctg
      781 cgaccgccgc gccggcgtcg aggtggccga tgttggcctt gacggaactc accgcgcagt
      841 atccattgcg gcgcgtcgtg cggcgaaagg cgcgcgtcaa ggctgctatt tcgatcggat
      901 cgccgatcgc cgtaccggtg ccgtgcgctt ccacgtagcc aatggtgtcg gccgatacgc
      961 cggcacaggc gagggcgtcg gagatggccg cggcttgtcc ggtgaggctc ggtgcggtga
     1021 agccgacttt cgccgagccg tcgttgttga tggcggaacc cttgacgagc gcgcgtacgt
     1081 tgtcgcactc gcgctgtgcg tcctccagcc ggcgcaggac gatgaccccc acgccgcttc
     1141 cccgaatcgt accgcgcgcg cccgcgtcga aagcgcggca gtgcccgtcg ggcgacagta
     1201 tcgcgccttg ctcgtagcga tagccggcgc gttgcggcac gagaatgcgt ccgccgccca
     1261 cgagcgcaac atcgcagtcc ccggcccgca ggctctggca cgcgagatgg agcgcaacgc
     1321 cggaggtcga gcacgcggtc tgcacgttga ccgccggccc gcgcaggttg agcttgtacg
     1381 ccacgcgggt cgccgggaag tccttctcgc ccgcgatcat ggcctgatat tcgccggcag
     1441 cctccagcac gtcgcggcgc gtagcgagca gatgcgcgag gtaggtattg cgcgcgacac
     1501 cggcgaagac gccgatcctg ccctcaaagg cggccggatc gattcccgcg tcctcgagtg
     1561 ccgcccatgc gcactccaga agcaggcgat gctgcggatc catgaggcgc gcttcggcgg
     1621 gattcacgcc gaagaaagcg gcgtcgaaac attccgcgtc gctcagcggt gcgcccgccg
     1681 gaacataatc ggggtgcgag agcagcgccg gatccacacc gcagcccgcg agatcctctg
     1741 cggtaaagcg aaccacggac tcgacgcgat tcgcaatgtt gccccacaac gtttcgacgt
     1801 caggcgcccc gggaaatctc ccggccattc cgacgatggc gatctcggcg cgatgtggcg
     1861 tcatcaggct cccatctcgc cggctgccgg cgccccgctt gctgcaagcg cgggagcggg
     1921 ccggtcgtat cccagcaact ccgcgagctg ccaggtcgcc tcggagagaa agttgtcctg
     1981 ctccacgccg cgccagcgcg ccgcggcccg cgggctgatg gactggtact ggaaaagatg
     2041 cgtatcgccc atcggggtgg aatcgggctt cagcccgtcg gtcatcttgc gctccagatt
     2101 gtcataaggg cgcaggagtt cctcgtcgaa agggataccg agacgcgcgc acatttcctg
     2161 catgacgaga tgcgggcgcg tgaccagctc ctcgtagcgt atggcgtact gccgacgctg
     2221 cgggatattg gcgaggaaat cgagaatgtt gcggttgctg aggagccata cgagctcgcc
     2281 cagctgccgg gggctgaagc ggtgcgctcc gagatagagc acctgctcca tgtggtagct
     2341 ctcgaacgag gtgaccatcg cgtagggatg gcgcacgaga tggatgtaga gcgggtcggc
     2401 aaagtcgcgc tcggcgcgtg cgagcgcgtt cggatccatt gcgtaagacg gcgatttgtc
     2461 caccagcatg cgattggcga tcgcgtcctg aaggtagcgg aagaatgcgc gcgtggtgag
     2521 cccctgctgc tcgagctctg ccatgatccg cttggcttcc tcgtcgctgc actggtgcac
     2581 ttccatgagg gcgcggattc cgccttcgag ccacaacgca tgagcgccct ggtacgcctc
     2641 gcggcgctct ttgagcgttt cgaagccgag a
//
LOCUS       DASURG010000433         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1301754, whole genome shotgun sequence.
ACCESSION   DASURG010000433 DASURG010000000
VERSION     DASURG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301754"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1549)
                     /gene="leuC"
                     /locus_tag="VFY80_07580"
     CDS             complement(143..1549)
                     /gene="leuC"
                     /locus_tag="VFY80_07580"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6007808.1"
                     /translation="MTSATMFEKIWRTHVIAEEDGEVLLHVDRALIHEGSSHAFAKLD
                     SQSRTVARPKQVFAFNDHYVPTSGREKGVEGIAIPEIRNMVIKLAENTAKHGITLFGI
                     DDERQGILHIVPPEQGITQPGLVICGADSHTSTHGAFGCLAFGIGASEMMHVMATQTI
                     WQRPSKTMRIAVNGRLGFGVTPKDVILAIIAKIGAGGGVGHVIEYAGSAITAMTMEGR
                     MTVCNMSIEAGARAGMVAPDETAFEYMQGRPYAPKGGAWDEALAFWRTLPSDTDARFD
                     REVALDGAGIAPMVTWGTSPEHASPVTETVPDPERAPDEAKRAEIAAALEYQGLTPGM
                     ALEEVKVDRVFIGSCTNSRIEDLRAAAEVARLGRARVPAWIVPGSRTVKRQAEREGLD
                     RIFREAGFEWRDPGCSLCTAINGDQLQPGERCASTSNRNFRGRQGTGSRTHLASPPMA
                     AAAALTGRLTDVRALLKQ"
     gene            complement(1561..2613)
                     /locus_tag="VFY80_07585"
     CDS             complement(1561..2613)
                     /locus_tag="VFY80_07585"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX6007809.1"
                     /translation="MGKKIVVVGAGAIGGYAGGHMTRAGHDVTFIDPWPEHVEYMRAH
                     GLQLSGLTPQETHSIRVNAMHLTDVQSLMKGRPVDIAFICMKSYDTEWATHLIRPYLA
                     PDGFCVSLQNCINEERIAGVVGWGKTLGCIASLVAAELYQPGHIKRTVPIGGDKHIVF
                     RVGEVHGRVTKRAEEVADLLRAADSAKVTTNLWGERWSKLVVNSMRNGLSAATGLNGN
                     ARDTAEGPRWLSIRLGSQAVRVGQALGYQLERMLGMDPETLARAGEGHRDALTEIIDI
                     LLESAKKRSDEQRPSMAQDIAKGRRTETDFINGYVAAKGDEIRVPAPIHAKMNEIVRR
                     VERGELRAMPDNIASL"
     gene            complement(2803..>3231)
                     /locus_tag="VFY80_07590"
     CDS             complement(2803..>3231)
                     /locus_tag="VFY80_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6007810.1"
                     /translation="EFPDILVNQRVVDALCMELVMKPETFDGLLLPNLYGDIVSDLAA
                     GLAGGLGVAPGANFGTGCAMFEAVHGTAPDIAGKDVANPMAMILSAVMMLRHINEARA
                     ADRIENGLKQQLREKREVTRDLGGEAGTQRMAAAVIEKLK"
BASE COUNT          478 a         1115 c         1010 g          628 t
ORIGIN      
        1 cgtgtcccgg tccgcgcggt accttgttaa agcgcgtggg gcaaatctgg ttggtatcca
       61 cgttcggctc gtcgatcggc gcggcgatgg cagtgagctg ggtaaacggt tccatggcgt
      121 atgtcgttct atgggcgttg attcattgct tcagcaacgc gcgcacatcg gtgaggcgcc
      181 ccgtcaacgc cgcagccgcg gccatcgggg ggctcgcgag atgcgtgcgg ctccccgtcc
      241 cctgccggcc gcggaaattg cggttcgacg tggaagcaca acgctctccg ggctgaagct
      301 ggtcgccgtt gatcgcggtg cacagcgagc accccggatc gcgccattcg aagcctgcct
      361 ccctgaagat gcggtcgagt ccctcccgtt ccgcctgccg ctttaccgtg cgcgacccgg
      421 gcacgatcca cgccggcacg cgggcgcgcc ccagtcgtgc tacttccgcc gccgcgcgca
      481 ggtcttcgat gcggctgttg gtgcaggagc cgatgaagac ccgatcgacc ttgacctcct
      541 cgagcgccat cccgggcgtg agcccttggt attcgagcgc cgcggcgatc tcggcgcgct
      601 tcgcctcgtc cggcgcacgt tccggatcgg gcaccgtctc ggtcaccggt gaggcgtgct
      661 cgggactcgt accccacgtc accatcggtg cgatgcccgc gccgtcgagc gccacttcgc
      721 gatcgaaacg tgcgtccgtg tccgaaggca gcgtcctcca gaaggcaagt gcctcgtccc
      781 acgcgccgcc cttcggcgca tagggacggc cctgcatgta ctcgaacgcc gtctcgtcgg
      841 gcgccaccat acccgcgcgc gcaccggcct cgatcgacat gttgcacacc gtcatgcgac
      901 cttccatcgt cattgccgtg atggcggaac cggcatactc gatgacgtgt cccacgccgc
      961 caccggcgcc gatcttcgcg atgatcgcaa ggatgacatc cttcggcgtc acgccgaacc
     1021 cgagcctccc gttcacggca atccgcatcg tcttcgacgg gcgctgccag atggtctgcg
     1081 tcgccatgac gtgcatcatt tcggaggcgc cgatgccgaa cgcgaggcag ccgaatgcgc
     1141 cgtgcgtcga agtgtgcgag tccgcgccgc agatcacgag cccgggctgg gtgatgccct
     1201 gctccggcgg cacgatgtgg agtatgccct gccgctcgtc gtcgatgcca aagagcgtga
     1261 tgccgtgctt cgcggtgttt tccgcaagtt tgatcaccat attccggatt tctgggatgg
     1321 cgatgccctc cacgcccttc tcgcgtcccg atgtcggcac gtagtggtcg ttgaaggcga
     1381 acacctgttt cggccgcgcg acggtgcggc tctggctgtc gagctttgcg aacgcgtgcg
     1441 aggagccttc gtgaatcagc gcgcggtcca cgtggagcag gacttcgccg tcttcttccg
     1501 cgatcacgtg cgtgcgccag atcttttcga acatcgtcgc cgatgtcata gctcgtccct
     1561 tcacaagctc gcaatgttgt cgggcatcgc gcgcaactct ccgcgctcca cgcggcgcac
     1621 gatctcgttc atcttcgcat ggatcggcgc gggcacgcgg atttcgtcac ccttcgctgc
     1681 aacgtagccg ttgatgaaat cggtctcggt gcggcgccct ttcgcgatat cctgcgccat
     1741 cgacgggcgc tgctcgtcgg agcgcttctt tgcgctttcg agcaggatgt cgatgatttc
     1801 ggtgagggcg tcgcgatgcc cctctccggc gcgggcgagc gtctccgggt ccatcccgag
     1861 catgcgttcg agctggtagc cgagcgcctg ccccacgcgc accgcctgcg aaccgagccg
     1921 gatcgaaagc cagcgcggcc cttccgcggt atcgcgcgcg ttgccgttga ggccggtcgc
     1981 tgccgacagg ccgttgcgca tcgagttgac tacgagcttt gaccagcgct cgccccagag
     2041 attggtcgtc accttcgcgc tgtccgcagc gcgcaggaga tccgcgactt cctccgcgcg
     2101 cttcgtcaca cggccgtgca cttcgccgac gcgaaagacg atgtgcttgt cgccgccgat
     2161 cggcacggtg cgcttgatgt ggcccggctg atagagttct gcggcgacca ggctcgcgat
     2221 gcagccgagc gtcttgcccc agcccacgac accggcgatg cgctcctcgt tgatgcagtt
     2281 ctggagcgag acgcagaagc cgtcgggcgc gagatagggc cggatcagat gcgtcgccca
     2341 ttccgtgtcg taggacttca tgcagatgaa ggcgatgtcg accggccggc ctttcatgag
     2401 gctctgcacg tcggtcaggt gcatcgcgtt cacgcgtatc gaatgcgtct cctgcggtgt
     2461 caatcccgag agctgcagtc cgtgcgcgcg catgtattcc acgtgctcgg gccacggatc
     2521 gatgaacgtc acgtcgtgtc ccgcgcgcgt catgtgcccg ccggcgtaac cgccgatcgc
     2581 gccagcgccc acgactacga ttttcttgcc catgtgcgat tcgttccgtg ctcgagggtc
     2641 ataccggcct tgctgcctca atcggccgcc tgtcatcggt cgtgccaccg caaccggccc
     2701 cgtcgtgtca gaccggccct gccgcctcaa tcggccccgt ccgtcagacc ggcgaaagcc
     2761 ggtagccagc cgtctagcgt taggctgttg aatgaaaccg gtctatttca gtttctcgat
     2821 gacggcagca gccatgcgct gcgttcccgc ctcgccgccc agatcgcgcg tcacctcgcg
     2881 cttctcacgg agctgctgct tcaagccgtt ctcgatgcgg tccgcggctc gagcttcgtt
     2941 gatgtgccgc agcatcatca ccgcggagag gatcatcgcc atcggattcg ccacgtcctt
     3001 gcccgcgata tcgggcgccg tcccgtgcac cgcctcgaac atcgcgcagc ccgttccgaa
     3061 gttcgcccct ggcgcgacgc cgagaccgcc ggcgagcccc gcggcgaggt cggagacgat
     3121 gtccccgtag agattcggca gcagcagccc gtcgaaggtc tcgggcttca tcacgagttc
     3181 catgcacagc gcgtcgacca cgcgctggtt cacgagaata tcggggaatt c
//
LOCUS       DASURG010000434         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_281531, whole genome shotgun sequence.
ACCESSION   DASURG010000434 DASURG010000000
VERSION     DASURG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281531"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..430
                     /locus_tag="VFY80_07595"
     CDS             56..430
                     /locus_tag="VFY80_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6007811.1"
                     /translation="MKHEDATTAFEKTSLGASNSAYEVIARLDPEYFEKLKGLYVDGT
                     FGRDGALSRKTKELIMVGITCALRAPRGVRIHSQRALSLGATSQEVLEAMEVAAIPGG
                     MPGLWLGVETLQELLEPRSDGK"
     gene            594..1535
                     /locus_tag="VFY80_07600"
     CDS             594..1535
                     /locus_tag="VFY80_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007812.1"
                     /translation="MLLVCVSPNVSSQGYPAKPIRVIDAYPPGGSTDVLARLVAPKFQ
                     EATGQPWVIENRPGAQGIIGCEYVARAAPDGYTLLMFTGSHTVHPSIYRKLPYDLLRD
                     FAPVTLASSTTNILVVHPTVPAKSLREFIALAQSARGKLNYSSAGTGSTTHMAAELLK
                     SMARIEATHIPYKGSAPAVVDLVGGHVDFMFAPLPVMLPHVKSGKARALAVSTAKRSA
                     ALPAIPTVAEAGLPGFEATNAVGVLAPAATPKAIVEKLNAEIVRVLKLPDIHERLVSM
                     GAEPVGSSTGAFAEFLRVDIARWAKVVKEAGIAVQTY"
     gene            1560..1976
                     /locus_tag="VFY80_07605"
     CDS             1560..1976
                     /locus_tag="VFY80_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6007813.1"
                     /translation="MKKLAVTPEEYVEILNLYSAYNHASDAGEAEWYASLFAEDGEHH
                     GTYDVKGRAALVEYKKKDKASRTHLYRRHWNGSIYLEKIDAQTIRGRCYLFGYNGEPG
                     KLPYATHAGVYTDTIKLVDGEWKFAERRLVFDGTLK"
BASE COUNT          403 a          631 c          662 g          336 t
ORIGIN      
        1 gcggatcgaa ccgccgcgaa tcgaccaatt ggatgcagcc aacaggaggc cgtggatgaa
       61 acacgaggac gcaacgacag cattcgagaa aacctcgctg ggcgcgagca acagtgccta
      121 cgaggtcata gccaggctcg atccggagta cttcgaaaag ctcaagggcc tgtatgtgga
      181 cgggacattc ggccgcgacg gcgctctttc ccgcaagacc aaggagctca tcatggtggg
      241 aatcacatgt gcgttaaggg cgccgcgcgg cgtgcgcatt cattcccagc gtgcattgag
      301 cctcggagca acgtcccagg aagtgctgga ggcaatggag gtcgcagcca ttcccggggg
      361 aatgcccgga ttgtggctgg gcgtggaaac actgcaggag cttctggagc cacggtcgga
      421 cgggaagtga ggcgggacgg gcgcactcat cctcggatgc ggatgcggat gcggtcagga
      481 ttgcgggctg ttgctttctc tgctcggatt gcaggcgatc cggtgcgcgc gtagactcgg
      541 cgggaaagaa gaggtgcaat ggtgtgatcc gctcctcggc cgctgctttc accatgctcc
      601 tggtctgcgt ctcgcccaac gtgagcagcc aaggctatcc cgcgaagccc attcgggtaa
      661 tcgatgccta cccgccaggc ggctccaccg atgtattggc ccggctcgtc gcgccgaaat
      721 tccaggaggc gactggccag ccatgggtga tcgagaatcg gcccggcgcg cagggcatca
      781 tcggctgcga gtatgtcgcg cgcgcggcgc ccgacggcta caccctgctc atgttcaccg
      841 gcagtcacac cgtgcacccg agcatctaca ggaagctgcc gtacgacctg ttgcgcgatt
      901 tcgcacccgt aacgctcgcc agctcgacca ccaacatcct ggtggttcac ccgaccgtcc
      961 cggcgaagtc gttgcgcgaa ttcattgcgc tggcacagtc cgcgcgcggc aagctcaact
     1021 actcttccgc aggtacgggc tcgaccaccc acatggcggc ggagctgctg aagagcatgg
     1081 cccgcatcga ggcgacgcac attccctaca agggctccgc gccggcggtg gtcgacctgg
     1141 tgggcggcca cgtggacttc atgttcgcgc cgttgccggt catgctgccg cacgtgaaga
     1201 gcggaaaggc cagggcgctg gcagtctcga ccgcgaagcg cagcgccgcc ttgccggcaa
     1261 tcccgactgt cgccgaagcc ggattgccag ggttcgaggc cacgaatgcg gtaggcgtcc
     1321 ttgcccctgc tgccacgccg aaagcgatcg tcgagaagct caacgccgag atcgtgcgcg
     1381 tgttgaagct gcccgacatc cacgaacgcc tggtgtccat gggcgccgag cccgtcggca
     1441 gctccaccgg ggcgttcgcc gagttcctgc gggtggacat tgcgcgctgg gccaaggtcg
     1501 tgaaggaagc cggaattgcg gttcagacct attgatcgtt caactctcct ggagaacaca
     1561 tgaagaagct cgcggtaacg ccggaggaat acgtcgagat tctgaatctg tactcggcct
     1621 acaaccatgc cagcgacgcc ggtgaggcgg agtggtatgc gagcctcttc gccgaagacg
     1681 gcgagcatca cggcacttac gacgtgaagg ggcgcgcggc gctggtggag tacaagaaga
     1741 aggacaaggc gagccgcacg cacctctatc gccggcactg gaacggaagc atctatctcg
     1801 aaaagatcga cgcgcagacg atccgcggac gatgctatct attcggctac aacggcgagc
     1861 ccggcaagct gccctacgcc acccacgccg gggtttacac cgataccatc aagctcgtcg
     1921 acggcgagtg gaaattcgcc gagcggcggc tcgttttcga tgggacgctg aagtgaacgc
     1981 ccgcagaaac gcctcgacca cgagtgccgc tcgggcacgc actacggcgg ct
//
LOCUS       DASURG010000435         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1302030, whole genome shotgun sequence.
ACCESSION   DASURG010000435 DASURG010000000
VERSION     DASURG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302030"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VFY80_07610"
     CDS             complement(<1..113)
                     /locus_tag="VFY80_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX6007814.1"
                     /translation="MQIGPYPLKNRLVVAPMAGVTDRPFRQLCKRFGAGLAV"
     gene            complement(271..1425)
                     /locus_tag="VFY80_07615"
     CDS             complement(271..1425)
                     /locus_tag="VFY80_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6007815.1"
                     /translation="MTESPGADAAIVGGGPVGTALAIALARSDVRAVLLESRVPATAP
                     TDPRPIALSYGSRLILERLNVWEALGRTTPIERIHVSQQGGFGHALMAADRAGLPALG
                     YVVEYARLQNALTAALQAARGFAVYGAQVTAIHGDHEKAAVDYHDAEGERTLIARVVI
                     IADGGAVSGATTKVIDYHQAAVVAVVSSELPHGGTAFERFTPEGPLALLPCGKDLALV
                     WSMKAEAAMACAAEPTDTFLARLHRAFGGRVGRFTAVGARLTYPLALRIGRAAMPRTL
                     YVGNASQTLHPVAGQGFNIGLRDAWELSAQIRDAERGTLGTSPMLEACEKRRRLDRRG
                     GTVFTDALVRIFSNDFPPLRAARGLGLAALDALPPAKDVLVRRMIFGARG"
     gene            complement(1418..>2267)
                     /locus_tag="VFY80_07620"
     CDS             complement(1418..>2267)
                     /locus_tag="VFY80_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HEX6007816.1"
                     /translation="PSEFRDVHAVLDEMRLVKDAAEVATMRRASEISATAHLRAMRAV
                     HPGASEYEIEAELLHEFRRSGAQAPAYTPIVAGGARACVLHYVENSAVLRDGELLLID
                     AGCELDGYASDITRTFPVNGRFGGPQREVYELVLAAQQAAIAAVKPGNAWIAPHEAAV
                     KVLARGMIDLGLLSGTLDEVLEKEAYKPFYMHRTGHWLGLDVHDAGNYRQAGAWRPLA
                     PGMVLTVEPGCYVRQGPDIPERYAGIGVRIEDDVLVTETGCEILTRDAPKTVRDIEAW
                     MRRNGD"
BASE COUNT          400 a          745 c          750 g          372 t
ORIGIN      
        1 acggcgagac cggctccgaa tcgtttgcac aactggcgaa agggccggtc ggtgacgccg
       61 gccatcggcg cgaccacgag tctgttcttg agcggatagg gaccgatttg catcggcgga
      121 ttcttgcgaa aaaggcagtc attctaattc cgggctgcgc gcaaggaaag aatggcgtgc
      181 gctttaccgt gaacgatgtt gcgacaaaaa cacagaacct gctgacgaag cgcggtggag
      241 tgagcgctta ttggaagtgc ggcggacgat tcatccacgc gcaccgaaaa tcatgcggcg
      301 aacgagaacg tcttttgcgg gtggcagcgc gtcgagcgcg gcgaggccaa gcccgcgtgc
      361 cgcgcgcagc ggtggaaagt cattggagaa aatgcgcacc agcgcatccg tgaacaccgt
      421 gccgccgcgc cggtccagtc gccggcgctt ttcgcatgcc tcaagcatgg gggaagtgcc
      481 gagcgtcccg cgttcggcgt ctcggatttg agccgaaagt tcccacgcat cgcgcagacc
      541 gatgttgaat ccctggccgg cgacgggatg cagcgtttgc gacgcgttgc ctacataaag
      601 cgtgcgcggc atcgccgcgc ggccgatcct gagcgccagc ggataggtca atcgcgcgcc
      661 gacagcggta aagcggccca cgcggccgcc gaatgcccga tgcaggcgcg cgagaaatgt
      721 atcggtgggc tctgcagcgc aggccatcgc cgcttcggct ttcatgctcc acacgagcgc
      781 cagatcttta ccgcatggca gcagcgcgag cgggccttct ggggtaaagc gctcgaaagc
      841 cgtcccgccg tgcggcagct cgctgctcac cactgccacc acggcagcct ggtgatagtc
      901 gatgaccttc gtcgtcgctc cggagacggc accgccgtcg gcgatgataa ccaccctggc
      961 gatgagcgtg cgctcacctt cagcgtcgtg gtaatcgacc gcagccttct catgatcgcc
     1021 gtggatagcg gtgacctgcg ccccgtacac cgcaaagccg cgggcggcct gcagcgccgc
     1081 ggtcaaggcg ttctgcaggc gtgcatattc gacgacgtag cccaaagcgg gcaatccggc
     1141 ccgatccgcc gccatcaggg catggccgaa gccaccctgc tgggaaacgt gaatgcgttc
     1201 gatcggcgtc gtgcgcccca aggcctccca gacattgagc cgctcaagga tcaggcggct
     1261 cccgtaggag agcgcgatcg gtcgcggatc ggtcggtgcc gtggcaggca cgcgcgattc
     1321 gagcagcacc gcacggacgt ccgaacgggc gagcgctatt gcaagcgccg tgccgaccgg
     1381 gccaccgccg acgatcgcag catccgcacc cgggctctca gtcaccgttg cgtctcatcc
     1441 acgcttcgat gtcgcggacg gtcttcgggg cgtcgcgcgt cagaatttcg cagcccgttt
     1501 cggtgaccag cacgtcgtcc tcgatccgca ccccgatgcc tgcgtagcgc tcgggaatgt
     1561 cggggccctg ccgcacgtaa cacccgggct ctacggtgag caccatgccg ggcgcgagcg
     1621 gccgccatgc cccggcctgg cggtaatttc ccgcatcatg gacatcgagt cccagccagt
     1681 gccccgtgcg gtgcatgtag aacggcttgt aggcctcttt ctccagaact tcgtccagcg
     1741 tgccgctcaa gaggcccagg tcgatcatgc cgcgcgcgag caccttgacg gccgcttcgt
     1801 gcggagcgat ccaggcattc ccgggcttga cggccgcgat ggcggcctgc tgggccgcga
     1861 gcacgagctc gtagacctcg cgctgcggtc cgccgaagcg gccgttcacc ggaaaagtcc
     1921 gcgtgatatc cgaggcgtag ccatcgagct cgcaacccgc gtcgatcaat aggagttcgc
     1981 catcacgcag caccgcgctg ttttcgacgt agtgcagcac gcatgcgcgc gcgcccccgg
     2041 cgacgatcgg agtgtaggct ggcgcctgcg ccccggagcg gcgaaactcg tggagcagtt
     2101 cggcctcgat ctcgtattcc gaggcgccgg gatgtaccgc ccgcatggcg cggaggtgag
     2161 cggtggccga gatttcggac gcgcggcgca tggtggcgac ttcggcggcg tccttgacga
     2221 gccgcatttc atcgagcacc gcatgcacat cgcggaattc ggacgga
//
LOCUS       DASURG010000436         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_689522, whole genome shotgun sequence.
ACCESSION   DASURG010000436 DASURG010000000
VERSION     DASURG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689522"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFY80_07625"
     CDS             complement(<1..1051)
                     /locus_tag="VFY80_07625"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX6007817.1"
                     /translation="MEQETRHWGYGAELAPGARALAPYAASRAPSRGRLYPEPDCPTR
                     SPLQRDRDRILHSTAFRRLNYKTQMFVFHEGDHYRNRLTHSLEVAQIARTIARQLCLD
                     EDLAEAIALAHDLGHPPFGHAGERALDEALHEVGGFDHNVQSFRIVTRLERKYAAFDG
                     LNLTWETLEGLVKHNGPPRGSEKPGDKVLVHAVRNFQQQMDLALDAWPTAEAQAAALS
                     DEIAWMTHDIDDGLRAGLIAFDDLRDLELLMPIVEHVERMPAIDSSRRIYGVTRQLIT
                     VLIADVVGESRARLARLAPEGPEDIRQAGGPVVSFSDPTEAAAKGLKAFLFKRLYRHA
                     RVMAVMDRAESIVRDL"
     gene            1198..1530
                     /gene="erpA"
                     /locus_tag="VFY80_07630"
     CDS             1198..1530
                     /gene="erpA"
                     /locus_tag="VFY80_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX6007818.1"
                     /translation="MTEANVTLSESAARRIMEIVADEPRNKLLRVSVEGGGCSGFQYK
                     FDLVAESEPDDIVVERNGAKVLIDPVSIMYMGGAEIDFVDDLIGSSFRINNPNATAAC
                     GCGTSFSI"
     gene            complement(1615..2184)
                     /gene="efp"
                     /locus_tag="VFY80_07635"
     CDS             complement(1615..2184)
                     /gene="efp"
                     /locus_tag="VFY80_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX6007819.1"
                     /translation="MAKVIASSVRKGNVLEIDGKLCVVLKAENIHPGKGTPVTHLEMR
                     RISDGVKVTERYRTTEQVERAYLDERELNYLYEDETGYTFMHPETYDQITVPKDVVGD
                     QAVYLQEGMTCTVSLYEGNPVSIELPPRVTLEVVEADPVVKGQTAASSYKPAVLSNGV
                     RVMVPPHITAGTRVVVSTVDGTYVERAKE"
     gene            2342..>2678
                     /locus_tag="VFY80_07640"
     CDS             2342..>2678
                     /locus_tag="VFY80_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX6007820.1"
                     /translation="MGASLRTRSSPWWDSGRHADRRPFLLARNRIKAKIRRWFEDRGF
                     TEVDTAILQVSPGNEAHLHAFETALTTPDGSRHPLYLHTSPEFACKKLLAAGEERIFT
                     FAPVFRNRER"
BASE COUNT          471 a          862 c          833 g          512 t
ORIGIN      
        1 caagatcgcg gacaatgctc tcggctctgt ccatcaccgc catgacccgg gcgtgccggt
       61 aaagccgctt gaacaggaaa gctttcagtc ccttggccgc cgcttccgtt ggatcagaaa
      121 aggacaccac cggaccgcct gcctggcgaa tgtcctccgg gccctcgggt gcaagcctcg
      181 cgagcctcgc ccgcgactcg cccacgacat cggcgatgag caccgtgatg agttgccgcg
      241 tcaccccgta gatccggcgc gagctgtcga tcgcgggcat ccgctccacg tgttcgacga
      301 tgggcatgag cagttcgaga tcccgcaggt cgtcgaacgc gatcaggccg gcgcggagac
      361 catcgtcgat atcgtgtgtc atccacgcga tttcgtcgga cagcgccgcc gcctgagctt
      421 ctgcggtcgg ccacgcatcg agtgccaagt ccatctgctg ctggaaatta cgaacagcat
      481 gcacaaggac cttgtcgccg ggcttctcgc tcccgcgcgg cgggccgtta tgtttgacga
      541 gaccttccag ggtctcccag gtcaggttga gcccgtcgaa cgccgcgtac ttccgctcca
      601 gtcgcgtcac aatgcggaag ctctgtacgt tgtggtcgaa tccgccgacc tcatgcagag
      661 cctcgtccag cgccctttcg cctgcgtgcc caaatggcgg atggccgagg tcgtgcgcca
      721 aagcgatggc ttccgccaga tcctcgtcga ggcagagctg ccgcgcgatc gttcgcgcga
      781 tctgtgccac ttccaggctg tgggtcagtc ggttgcggta atgatcgcct tcgtggaaga
      841 cgaacatctg cgtcttatag ttgagccggc ggaacgccgt ggaatgcagg atgcggtcgc
      901 gatcccgctg gagcgggctc cgcgtgggac agtcaggctc gggatagagg cggccccggc
      961 ttggggcacg gcttgccgca tagggcgcca gggcgcgggc gccgggagcg agctccgcgc
     1021 catatcccca gtgcctcgtt tcctgctcca tgagttcggc cacttggacg atcggcctaa
     1081 tgcgcttatc ttcagttccg tccacgtgat aggcgcgatt cttgccgggg tgcaaatcag
     1141 gtccacccga ggctatcggt cgcgccagtc cgttcgagat cgatcctgag caacgccatg
     1201 accgaagcaa acgtcactct gagcgaaagt gcagcccgcc gcatcatgga gatcgtggct
     1261 gatgagccgc ggaacaagct gctgcgcgtc agcgtggaag gcggcggctg ttcaggcttt
     1321 cagtacaaat tcgatctcgt ggccgagagc gagccggatg acatcgtggt cgagcgcaac
     1381 ggagcgaagg tgctgatcga cccggtgtcg atcatgtaca tgggcggggc cgagatcgac
     1441 ttcgtcgacg acctcatcgg atcgtcgttc aggatcaaca atcccaatgc gaccgcggca
     1501 tgcggatgtg ggacgagttt ttcgatttga aggtgaaatc gccaagtagt gaaatcgtga
     1561 aatcgaaaaa acacgacccc acgacttcac tagctcacga tttcacgacc cgattcactc
     1621 tttcgcccgc tccacgtacg tcccgtccac cgtcgacacg acgacgcgcg tgccggccgt
     1681 gatgtgcggc gggaccatga cgcggacgcc attcgagagc actgccggct tgtaggatga
     1741 agccgccgtc tggcccttca ccaccggatc ggcctcgacc acctccagcg tgacccgcgg
     1801 cggtagctcg atggagaccg gattgccttc gtagaggctc accgtgcagg tcatgccctc
     1861 ctgcagatag accgcctgat caccgacgac gtccttcgga acggtgatct ggtcgtaggt
     1921 ttccgggtgc atgaaggtgt agccggtctc gtcctcgtaa aggtagttca gctcgcgctc
     1981 gtcgaggtag gcgcgctcga cctgctcggt cgtccggtag cgctccgtta ccttgacgcc
     2041 gtcggaaatg cggcgcatct ccaggtgcgt caccggcgtg cctttgccgg gatggatgtt
     2101 ctccgccttg aggacgacgc acagcttgcc gtcgatctca agcacgttgc ccttgcggac
     2161 cgagcttgca atgacctttg ccacgtgttt ctccgttcgg ttgagcgggg tccgcggccg
     2221 gcgcgagcgc gcggcgttgc gggactgcat gcgatgtgcc ggggttcata gcgtgatcga
     2281 ggcgcgaggc caaggccagg cgtgccgcat cgtcgagttg cagcaggaac agagactctg
     2341 aatgggagcc tcactcagaa ccagatcctc cccctggtgg gactcgggcc gccatgccga
     2401 ccggcgccca ttcctgcttg cgcgcaatcg catcaaggcg aagatccgcc ggtggtttga
     2461 agatcgcggc ttcactgagg tcgacaccgc catcctgcag gtttctcccg gcaacgaggc
     2521 ccacctgcac gccttcgaga cggcgctgac aacgcccgat ggcagccgcc acccgcttta
     2581 cctccacacc tcccccgagt ttgcctgcaa gaagctgctc gccgcgggag aggagcgcat
     2641 cttcaccttc gcacccgttt tccgcaatcg cgagcgcg
//
LOCUS       DASURG010000437         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_178755, whole genome shotgun sequence.
ACCESSION   DASURG010000437 DASURG010000000
VERSION     DASURG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178755"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFY80_07645"
     CDS             <1..861
                     /locus_tag="VFY80_07645"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX6007821.1"
                     /translation="KAVHPDAEQAADGTFRMDGYRPAERHKDLYRGCLFPRKPNMLSV
                     AEIVKQKPAWAEVLKLRQSEQSRAMLDAIHAGTQEEIRLRAAMMASVEEGVGLLFEAL
                     EATGQLDNTFVLFLGDNGYFFGEHGLGPERRFAYEEGIRSPFIARFPKRIRAGTRVSD
                     LVICQDIAPTLIELAGGKPGPQIQGRSLLPLVAGKRSGWRKSFLIEYWAENAYPWLIG
                     MSYKAVRDARYKFIHWINRGRQGELDELYDLEKDPYEVRNLARRPAYRAVRERLTREL
                     KRLTAEAIGL"
     gene            939..1901
                     /locus_tag="VFY80_07650"
     CDS             939..1901
                     /locus_tag="VFY80_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007822.1"
                     /translation="MRGLRWLAALALALAATAAAAQNYPTKPVRIIVPFAPGGATDIV
                     TRILAQKLTEAWGQTVVVDNRAGAGGNIGADIAAKSPPDGYTLFMPSGSVMTANQHIY
                     KKISFNAEKDFVAITNVASGPQVVAVTNTMPVKTLKDLIALAKARPKEITFGSAGFGT
                     QTHLAAENFLYSAKVEATHIPYKGEGPALTDLAGGQIQLLTPNLSAAMSFLTSGKIRA
                     LGVTSKTRAPQLPNVPAVAEVVPGFENLGWFGLVAPAGTPAAIVKKVHADAVKALQSA
                     DIRKRFGELGMVPVGNTPDEFAKAMKEETARWAKVVRERKLQLD"
     gene            1970..2845
                     /locus_tag="VFY80_07655"
     CDS             1970..2845
                     /locus_tag="VFY80_07655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX6007823.1"
                     /translation="MKVDLTQVEETCKALYIRALKVLPPDIKQGFERLDRTETNATGK
                     AILGTMIRNIQVAEDTNNLLCQDTGIPIYNVAIGRGIEVDGYALKQAIVKGCERATRE
                     HPLRSSVVHPVTRRNEHTSCGRAIPVINIDFVPDAETLSIEMIPKGSGSENNSWLRMA
                     VPADGLDAIKTFVVDCVLDAGGKTCPPTIVGVGVGGTADLCVHLSKVAATRPLGTRCE
                     DPEGAKLEEDLSAVVNQLGVGPQGLGGDSTSFAVHVETAATHITMNPVAVNMQCHSAR
                     RARATFSPRGVEYGF"
     gene            2853..>3451
                     /locus_tag="VFY80_07660"
     CDS             2853..>3451
                     /locus_tag="VFY80_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6007824.1"
                     /translation="MAHHDFNMPVTEAQIRALRVNDTVTLNGTLYGIRDATQIHMFDH
                     GRKVRFDMKGHAVIHTAPNVRWVGETQATPCGYAPICIGTTTSARMERFTRPLMSEYG
                     VRMIIGKGGLGQGSLDAFAELGGVYLAIVGGAAALETTWVEAIEEVDLDDLNPESLWK
                     FRVRGFGPLLVSMDSHGGSLHTAVNQSARDKRAAALAALG"
BASE COUNT          669 a         1098 c         1116 g          568 t
ORIGIN      
        1 aaggcggtgc atcccgacgc ggagcaggcg gcggacggca cgtttcgcat ggacggctat
       61 cgccccgccg aacggcacaa ggatctctat cgcggttgcc tgtttccgag gaagcccaac
      121 atgctgtccg tggcagagat cgtgaagcag aagccggcat gggccgaggt cctgaagttg
      181 cggcagagcg agcagtcgcg cgcaatgctc gatgcgatcc acgccggcac gcaggaagaa
      241 atccggctgc gcgcggcgat gatggcctcc gtcgaggagg gcgtcggcct gctattcgaa
      301 gcgctcgaag cgaccggtca gctcgacaat accttcgttc tcttcctcgg cgacaacggc
      361 tattttttcg gcgagcacgg cctcgggccg gagcgccgct tcgcctatga ggagggcatc
      421 cgctcgccct tcatcgcgcg ctttccgaag cgcatccgcg cgggcacacg cgtctcggat
      481 ctcgtcatct gccaggacat cgccccaacg ctcatcgagc ttgcgggcgg aaagccggga
      541 ccgcagatcc aagggcgttc gctgcttccg cttgtcgccg gcaagcgttc aggctggcgc
      601 aaatcgtttc tcatcgagta ctgggccgag aacgcgtacc cgtggctgat cggcatgtcg
      661 tacaaggcgg tgcgcgatgc gcgctacaag ttcatacatt ggatcaaccg cggccggcag
      721 ggcgagctcg acgaactcta cgatctcgaa aaggatccgt acgaggtgcg gaacctcgca
      781 cgccgcccgg cgtatcgggc ggtgcgcgag cggctcacgc gggagctcaa gcggcttacc
      841 gctgaagcga tcgggctttg aagcgtctgg cttgccgctc gatgcccgat tggagtaagg
      901 tgcaagctcc tgattacaac ggataacgtg gaggaaaaat gcgtggactg agatggcttg
      961 cggcgctcgc gttggcgctc gccgcaacgg cggccgctgc acagaactat ccgaccaagc
     1021 cggtgcgcat catcgtgccc ttcgcacccg gcggcgcgac cgatatcgtg acgcgcatcc
     1081 tggcgcagaa gctgacggaa gcgtggggcc agaccgtcgt cgtggacaac cgcgccgggg
     1141 ccggcggtaa catcggtgct gacattgccg ccaagtcgcc gcctgacggc tatacgcttt
     1201 tcatgccttc gggctcggtc atgaccgcga accagcacat ctacaagaag atctccttca
     1261 acgccgaaaa ggacttcgtc gcgatcacca atgtcgcgag cggtccccag gtcgtggcgg
     1321 tgaccaatac catgccggtg aaaacgctca aggatctcat cgccctcgcc aaggcgcggc
     1381 ccaaggagat cacgttcggc tcggccggat tcggcacgca aacccacctc gctgcggaaa
     1441 actttctcta cagcgccaag gtcgaagcga cgcacatccc ctacaagggc gagggccccg
     1501 cgctcaccga tctcgcgggg gggcagatcc agctcttgac gcccaatctc tcggccgcga
     1561 tgagctttct cacttcgggc aagatccggg cgctcggggt gacgagcaag acacgcgcgc
     1621 cgcagctccc gaacgttcct gcagtggcgg aggtcgtgcc gggattcgag aacctcggct
     1681 ggttcggtct tgtcgcaccg gcagggacgc cggccgccat cgtgaagaag gtccacgccg
     1741 atgcggtgaa ggcgcttcaa agcgcggaca tcagaaaacg cttcggcgag ctcggcatgg
     1801 tgccggtcgg gaatacgccc gacgagtttg cgaaagcgat gaaggaggaa acggcgcgct
     1861 gggcgaaggt tgtgcgcgag cgcaagctgc agctcgattg agttccgtat acttcgtttc
     1921 gcctgagccg agcgaagtca agtcgaaggg cattggaaac gggaatggaa tgaaggtcga
     1981 tcttacgcaa gtcgaagaga cgtgcaaggc gctctacatc cgcgcgctca aggtgctgcc
     2041 gccggacatc aagcagggat tcgagcgcct cgatcgcact gaaacgaacg cgaccggcaa
     2101 agcgatcctc ggcacgatga tccgcaacat ccaagtcgcc gaagacacga acaatctgct
     2161 ctgtcaggac accggcatcc cgatctacaa cgtcgcgatc ggccgcggca tcgaagtgga
     2221 cggctatgcg ctcaagcagg ccatcgtgaa gggttgcgag cgcgcaacgc gcgagcatcc
     2281 gctgcgctct tcggtcgtgc atcccgttac gcgccgcaac gagcacacgt cctgcggtcg
     2341 tgcgattccg gtgatcaaca tcgatttcgt gcccgacgcg gaaacgttgt caattgagat
     2401 gattccgaaa ggctcgggat cggagaacaa ctcgtggctc aggatggccg ttcccgcgga
     2461 cggcctcgac gccatcaaga ctttcgtggt cgattgcgtg ctcgacgccg gaggcaagac
     2521 ctgtccgccc accatagtcg gcgtcggcgt cggcggaacg gccgatctgt gcgtccacct
     2581 ctccaaggtc gcggcgacac gtccgctcgg cacccgttgc gaggatcccg agggcgcgaa
     2641 gctcgaagag gacctgagcg cggtggtcaa tcagctcggc gtggggccgc agggcctcgg
     2701 cggcgattcc acctctttcg cggtacacgt ggagaccgcg gcgacccaca ttacgatgaa
     2761 cccggtcgcc gtcaacatgc agtgtcactc cgcccggcgc gcgcgcgcga cgttctcgcc
     2821 gcgcggggtc gaatacggat tctgacctca atatggcgca tcacgacttc aacatgccgg
     2881 tcaccgaagc gcagatccgg gcgctgcgcg tcaacgacac ggtgacgctc aacggcacgc
     2941 tctacggcat ccgcgacgcg acgcagatcc acatgttcga ccacggccgc aaggtgcgct
     3001 tcgacatgaa gggccacgcg gtcatccaca ccgcgccgaa cgtcaggtgg gtgggagaga
     3061 ctcaggcgac gccgtgcggc tatgcaccga tttgcatcgg cacgaccacc agcgcgcgca
     3121 tggagcgctt cacccgcccg ctcatgagcg agtacggcgt gcgcatgatc atcggcaagg
     3181 gcgggctcgg acagggctcg ctcgatgcgt tcgcggagtt aggcggcgtc tatctcgcca
     3241 tcgtcggcgg cgcggccgca ctcgagacga catgggtgga agcgatcgaa gaggtcgatc
     3301 tcgacgacct caaccccgaa tcgctgtgga aattcagagt gaggggtttc ggcccgctgc
     3361 tcgtttccat ggacagccac ggcggcagcc tccataccgc ggtcaaccag tcggcgcgcg
     3421 ataagcgtgc cgcggcgctc gccgcgctcg g
//
LOCUS       DASURG010000438         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1405294, whole genome shotgun sequence.
ACCESSION   DASURG010000438 DASURG010000000
VERSION     DASURG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405294"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFY80_07665"
     CDS             <1..359
                     /locus_tag="VFY80_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6007825.1"
                     /translation="LPTEAVERIVAIMQDRGLTAKISSIHVNGWFGTYDKLAMTLRMA
                     RECFAVELDREREHYIFIGDSPNDAPMFGYFPHAIGVANVRDFESELAHRPTYVAGKR
                     CGAGFRECVDFLLASK"
     gene            393..1424
                     /locus_tag="VFY80_07670"
     CDS             393..1424
                     /locus_tag="VFY80_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX6007826.1"
                     /translation="MIDLRSDTVTRPTEAMLESMREATFGDDSRDGDPTVMKLEAVAA
                     ERLGKEAGVFMPSGTMANLVAMMAHAQRGGEVLLEQGSHTLYAELGGIAGIAGLFYRG
                     LPGHRGAMDLEALREALRPTLRNQMGTALVWMENTHNRAGGAVLPEHHMQSVYALASD
                     HAVPVHLDGARIFNAATALATEARQLAQHADSVCFCVSKGLSAPVGSVLCGTAAFIER
                     ARAFRRMVGGNMRQAGPLAAAGIVALETMVHRLAEDHRTAKRLAEGFHRIDASLVAPG
                     EVETNLVRVEVKTGGRRAAEWSRALKDHGILVSPCDPYALRFVTHRHIGDAEVDAALA
                     AFATLWSGR"
     gene            complement(1554..1766)
                     /locus_tag="VFY80_07675"
     CDS             complement(1554..1766)
                     /locus_tag="VFY80_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX6007827.1"
                     /translation="MERKRVSASNIRSVGYDARNQILEVELRAGTIVQYSGVSETVYR
                     GFMNAPSPDSYYRDRIEDDYPAKRLR"
     gene            complement(2504..>2788)
                     /locus_tag="VFY80_07680"
     CDS             complement(2504..>2788)
                     /locus_tag="VFY80_07680"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX6007828.1"
                     /translation="SRYRNRRSSLHTVTELRAEENYTLWLKFDDGLEGRVYLGELVET
                     TSFGVLSDAEMFGRAAIDPVSNEVTWGAGLRIDPEVLYRDLMSKAAAALH"
BASE COUNT          459 a          908 c          888 g          533 t
ORIGIN      
        1 gactccccac ggaagcggtc gaacgcatcg tcgcgataat gcaggaccgg ggacttacgg
       61 cgaagataag ctcgatccac gtgaacggct ggtttggcac gtacgacaag cttgcgatga
      121 cgctgcgcat ggcgcgtgag tgcttcgccg tcgagctcga ccgcgagcgc gagcactaca
      181 tctttatcgg agattctccc aacgatgcgc cgatgttcgg ctattttccc catgccatcg
      241 gtgtggcgaa cgtgcgcgat ttcgaaagtg agctcgcaca ccgcccgacg tacgtcgccg
      301 ggaagcggtg cggcgcgggc tttcgcgagt gcgtcgattt cctgctcgcg tcgaagtaat
      361 atccgcgctc gaattgcgca gaaggtgaga ccatgatcga tctcagaagc gataccgtca
      421 cgcgccccac cgaggcgatg ctcgaatcga tgcgtgaggc gactttcggc gacgactcgc
      481 gtgacggtga cccgaccgta atgaagctgg aagccgtggc ggcggaacgc ctgggcaagg
      541 aggccggggt tttcatgccg agcggcacca tggcgaatct cgtggccatg atggcgcacg
      601 cgcagcgcgg cggcgaagtg ctgctcgagc agggatcgca taccctctac gcagagctcg
      661 gcggaatcgc cggtatcgcg gggctcttct accgcggcct acccggccac cgcggcgcga
      721 tggacctcga agcgctgcgc gaagcgctgc gacccacgct gcgcaaccag atgggcacgg
      781 cgctcgtctg gatggaaaac acgcacaacc gcgcgggggg cgctgtcctt cccgagcatc
      841 acatgcaatc ggtctatgcg ctcgcgagcg atcacgccgt gccggtgcac ctcgacggcg
      901 cgcgcatctt caatgctgcg acggcgctcg cgactgaagc gcggcagctc gctcagcacg
      961 ccgactccgt ctgcttttgc gtatcgaaag ggctttccgc gcccgtcgga tcggtgctct
     1021 gcggaacggc ggccttcatc gagcgcgcac gcgccttccg tcgcatggtt ggcggcaaca
     1081 tgcgccaggc gggaccgctc gccgccgcag gcatcgtcgc gctcgaaacg atggtgcatc
     1141 ggctcgccga ggatcatcgc acggcgaaac ggctggccga gggatttcac cgcatcgatg
     1201 cgagcctggt cgctccgggc gaggtggaaa cgaacctggt gcgcgtcgaa gtgaagacgg
     1261 gcggccggcg cgccgccgaa tggtcgcgcg ccctcaaaga ccacggaata ctggtgagcc
     1321 cgtgcgaccc ctacgcgctg cgcttcgtca cccaccgcca catcggcgat gcggaagtgg
     1381 acgcggcgct cgctgccttc gcaacgctct ggagcggccg ctgacctgat ctttgcggcc
     1441 ctgtgcactt cgcgcagccc agttgcgacg tattcggcac gagctcgcgc caatcgcgaa
     1501 gcactcgtat gtcgaggcgt ttgtggtgag cttgccagga acggactgcg ccttcaacgc
     1561 aatcgcttgg cggggtagtc gtcctcgatg cggtcgcgat aatagctgtc gggagacggc
     1621 gcgttcatga atccgcggta caccgtctcg gatacacccg agtactgcac gatcgttccc
     1681 gcgcgcagtt ccacctcgag tatctggttg cgcgcgtcgt atccaaccga gcggatgttc
     1741 gatgcgctca cgcgtttgcg ttccatgacg atctctcctg ctccagcaat gtgccgcggg
     1801 cgatgcacgc agcgcggtta gtatgaccga gcgcgcgctg caggcgtcac tgctcaagaa
     1861 ataggcagtc cacaatgtgc gagagcgccg gctcgggcca cacccgcaga ggcccagtcc
     1921 aacgtcatga ttgaatggaa gtattttgga gcgctgtcac ggggagcgcc ccgccggcca
     1981 aactttgctt gcgggcgggg ggcagatttt gtatctttag cgctctgctg tcagccggtc
     2041 aaaatccaac gcccgcggcc ccgcatgcct ccttgcggag gcggcatgac cgcaggcgtg
     2101 gacgggctta cggattgttc cccatgggga acggccatgc acccgggatt gcgaccggtc
     2161 ggggcgcttc cggaacgggt ggagcttccg ctgtttgagc gggcttcgcc gccggtctgg
     2221 gcgccgcctt ttttctgcca gcaggctttt tcttggctgc ttttttactg gccggctttc
     2281 gagccggcct tttttttgcc ggccgtctgc gcgccgccgt cttgcgcaga gccgccttgc
     2341 gcgccacttt ctttgctacg acctttcttt tcttcttcgc ggccttcttt ttcgcagctt
     2401 tcttccttgc catatcgagc tccttcgaga atgttgccac actcgccgct tcacgcagcg
     2461 accacttcgc agcgcgcggg agaggccccc gcggcgctgt ccctcagtgc aaggccgcgg
     2521 ctgccttgct catgagatcc cggtagagca cctccggatc gatcctgagc cctgcgcccc
     2581 aggtcacctc gttcgacacc gggtcgatcg ccgcacggcc gaacatttcg gcgtcggaga
     2641 gcacgccaaa gctcgtggtc tcgacgagct cgccgagata gacccggccc tccagcccgt
     2701 catcgaactt gagccacagc gtgtaattct cctcggcgcg gagctccgtg acggtatgca
     2761 agctcgacct gcggttgcgg taacggct
//
LOCUS       DASURG010000439         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1405426, whole genome shotgun sequence.
ACCESSION   DASURG010000439 DASURG010000000
VERSION     DASURG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405426"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFY80_07685"
     CDS             complement(<1..417)
                     /locus_tag="VFY80_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6007829.1"
                     /translation="MIRFDKVSKRFRIPPGPRRKAGVVDALLEVSAHIAAGEVIAVAG
                     PNGAGKSPLFSLLLGFLEPTGGELSIAGDDPRSYVRKHGAGYLPERFRLPPEWRVGDV
                     LRAFASIDRISRLRAQDTLERFGLAGHAQKNVAALSH"
     gene            496..1191
                     /locus_tag="VFY80_07690"
     CDS             496..1191
                     /locus_tag="VFY80_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6007830.1"
                     /translation="MRGFGGALAAFLTVGIMVAIAAVGIFAGIAEAVEEGFTQPFDES
                     VLRWFESTRAPWLDRIMLELTTLGSGVVLILLVLITSVVLWQTQHKWSVFLLLLGTIG
                     GKLLNTALKLAYNRPRPTIVEAVTDAYSPSFPSGHAMSAMVVYSCVAYLVGRLEPARG
                     LRYTVWIFAALLILAIGISRMYLGVHYPSDVLAGFAGGLAWLGFVIAGMQALQHFARR
                     RPETLREEKDLNQ"
     gene            1252..2760
                     /locus_tag="VFY80_07695"
     CDS             1252..2760
                     /locus_tag="VFY80_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX6007831.1"
                     /translation="MNNSRVLGQLLIAAGHISEEQLVKALRRQRTTRERLGEILIKEG
                     LPARCIAQALAQQLRLHYADPPLQPEPAAFQLIDRSTAARLRVLPLSVREKTVRVAMA
                     DPLDMSAVDDLQFRTGKRVEPVVAEPAAIESALAAFESEAPTVVATVEHILRSAVQAR
                     ASDIHIEPAAEAVVVRGRVDGVLRELLTLPKQTNAAVVSRIKVMAALDISVKRKPQDG
                     RTSVRVNGRDISARISTLPANGGEKVVVRLLDGGEHFASLDDLGLSEQSLNLLRAMLQ
                     HPHGVVLVTGPTGSGKSTTLYAALSELDRQTRNIITLEDPVEYKIAGITQVQVDRRAG
                     LTFPRVLRAVLRQDPDIIMIGEMRDRETVEVALAAAMTGHLVLSTLHTNDAPSAVARL
                     FDMGAPPYLVAASLVGVVAQRLVRRVCPACRTDGCSACQGSGFRGRVGVYEILPINPE
                     LAALIGRRTSAQAIRKAGRDNGMTSLAEDARRKVDLGLTTAAEAAPLLGLAD"
BASE COUNT          510 a         1011 c          933 g          519 t
ORIGIN      
        1 gtgcgagagc gccgccacat tcttctgcgc gtgaccagcc aggccgaatc gctcgagcgt
       61 atcctgtgcc cgtaaccgcg aaatgcggtc tatgctggcg aaggcgcgta acacgtcgcc
      121 cacacgccac tccgggggca accgaaagcg ctcgggcaaa tagcccgcgc catgcttgcg
      181 cacgtagctg cgcggatcgt cgccggcaat gctgagctcg ccgccggtgg gctcgaggaa
      241 gccaagcagg agggaaaaga gcgggctctt gcccgcgccg ttgggtccag ccaccgcaat
      301 cacttcgccc gccgcaatgt gcgcgctgac ctcgagcagc gcgtcgacca cacccgcttt
      361 gcgccgcgga ccgggcggaa tgcggaatcg cttggagact ttatcgaatc gtatcacgcg
      421 tacccgtgag gcatgtatcc tgccagccgc tcaactttgg tgattcattc tatcctgcgt
      481 tttatcggcc ggcacgtgcg cggcttcggc ggtgcactgg cagcgttcct gaccgtcggc
      541 atcatggtcg ccatcgccgc tgtcggcatt ttcgccggaa tcgccgaggc cgtggaagaa
      601 ggcttcacgc agcccttcga cgaatccgtg ctgcgctggt tcgaaagtac gcgcgcgccc
      661 tggctggacc gcatcatgct cgagctaacg acgctgggct ccggcgtggt actcatcctg
      721 ctcgtgctga tcacctcggt cgtcctctgg cagacgcaac acaagtggtc ggtgttcctg
      781 ctgctgctgg gcaccatcgg cggcaagctg ctcaatactg cgctcaagct tgcctacaat
      841 cggccgcgcc ctaccatcgt ggaagccgtg acggacgcat actcgccgtc cttcccttcc
      901 gggcacgcca tgtccgcgat ggtcgtctac agctgtgtcg cctacctggt aggacgcctg
      961 gagcccgccc gcgggttgcg atacaccgtg tggatctttg cagcgctgct catcctggcg
     1021 atcggcatca gccgcatgta cctgggcgtc cactatccgt ccgacgtgct ggctggcttt
     1081 gcgggagggc tggcctggct cggtttcgtg attgcgggca tgcaggcgct ccagcacttc
     1141 gcgcgacgtc gtccggaaac actccgggag gagaaagacc tgaaccagtg agcgccggtt
     1201 acggcgcatc atcccatggc tccgcccagc acctgacgcc atgctgcgcg tatgaataac
     1261 agccgggtgc tcgggcagct gctgatcgcg gccggccaca tcagcgaaga acaactcgtc
     1321 aaggcactgc gccggcaacg caccacgcgt gagcgcctgg gcgaaatcct gatcaaggaa
     1381 ggactgcccg cgcgctgcat cgcgcaggcc ctcgcccagc aattgcgcct gcactatgcg
     1441 gatcctcccc tgcagcccga gcccgccgcg tttcagctga tcgatcggtc gacggcggca
     1501 cgcctgcgcg tattaccgct ttcggtacgc gagaaaacgg tgcgcgtcgc catggcggac
     1561 cccctcgaca tgagcgccgt cgatgacctg cagttccgaa ccggtaagcg cgtcgagccg
     1621 gtggtcgccg aaccggcggc catcgaaagt gcgctcgcag ctttcgagag cgaagcaccg
     1681 accgtggttg ccacggtcga acatatcctg cgatcagcgg tccaggcccg cgcgagcgac
     1741 attcatatcg agcctgcagc cgaggccgtt gtcgtgcgcg gccgcgtaga tggcgtgctg
     1801 cgcgagctgc tcacattgcc caagcagacg aatgccgccg tcgtctcacg catcaaggtc
     1861 atggctgcgc tcgacatctc cgtcaaacgc aaaccgcagg acggtcgcac gagcgtgcgc
     1921 gtcaatggtc gcgacatctc ggcgcgcatt tcgacactcc ccgccaacgg cggagagaaa
     1981 gtcgtcgtgc gtctgctcga cggcggcgag cacttcgcat cactcgacga cctcgggctc
     2041 tcggagcagt cgctcaacct gctgcgtgcc atgctccagc atccgcacgg tgttgtcctg
     2101 gttaccggtc ccacaggctc gggaaaatcc accacgctct acgctgcact cagcgagctg
     2161 gaccgacaga cacgcaacat catcacactg gaagatccgg tggaatacaa aatcgcaggc
     2221 atcacccagg tgcaggtcga caggcgcgcc gggctcacct ttccccgcgt gctccgcgcc
     2281 gtgctccgcc aggatccgga tatcatcatg atcggtgaga tgcgtgaccg cgaaacggtc
     2341 gaggtcgcgt tggccgcagc catgaccggc cacctcgtcc tgtcgacact gcacaccaac
     2401 gacgctccat ccgccgtcgc acggctgttc gacatgggcg ccccacctta cctggtggcc
     2461 gcatccctgg tcggcgtggt ggcgcagcgg ctggtgcgca gggtctgccc cgcctgccgg
     2521 acggacggct gctcggcgtg ccagggatcc ggcttccgcg gcagggttgg tgtctacgaa
     2581 atactgccca tcaaccccga gctggccgcc ctgatcggcc gcaggacgag cgcacaggcg
     2641 attcgtaaag ccggccgtga caacgggatg acgtcgctgg ccgaagacgc tcgccgcaag
     2701 gtcgacctgg gcctcaccac ggccgcggaa gcggccccgc tcctcggcct cgccgattag
     2761 ctgtccggtt tcgcacgccg aggcgttctt catgtgggca gttatctcag taccaaaata
     2821 tctggaggag ctatgtgtct tcgctctacg gcgggtctgg cactcgtcat cctcgcagca
     2881 tgtggcggcg ggggtggcgg cggaggcggt gggccggtgc agccgggccc gacgaccggc
     2941 agcatacgcg gaagcgtgac cgacaacacg gcc
//
LOCUS       DASURG010000440         5922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1302569, whole genome shotgun sequence.
ACCESSION   DASURG010000440 DASURG010000000
VERSION     DASURG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302569"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFY80_07700"
     CDS             <1..721
                     /locus_tag="VFY80_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6007832.1"
                     /translation="VERLRETLAQVRVLAAEMERNGAALKSLAVEATSEKRRLEGEKD
                     ARSRVLARLSREIDQQRREIGTLKRNESRLARLIEELGRVVVRRPSSPRLSNQRVPGR
                     AEPSGPFEALKGRLKLPVRGELANRFGSPRREGALTWKGLFITARAGDEVRAIAAGTV
                     VFADWLRGFGNLLIIDHGGAYMSLYGYNQALLKRVGEAIRGGDPIATVGNSGGNADSG
                     LYFELRHEGRPLDPLKWVDIQ"
     gene            758..2185
                     /locus_tag="VFY80_07705"
     CDS             758..2185
                     /locus_tag="VFY80_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6007833.1"
                     /translation="MRNRLHHIGLIFLGILMGVAISLNFSAGAQKDAVLAPLPIEELR
                     AFTEVFGRVKSDYVEPVEDKRLITQAINGMLTGLDPHSAYLDQEAFRELQVGTQGEFG
                     GLGIEVGMEEGFVKVIAPIDDTPAARSGIKAGDLIIKLDDASVKGMTLTDAVKRMRGK
                     PNTQITLTIVRKGEDKPLVVTVTRAIIKVQSVKAKLLEPGYGYFRVSQFQEHTGENLA
                     AAITRVFKENQGSMKGIVLDLRNDPGGLLNGAVAVSAAFLPSGSLVVYTDGRTEDSKM
                     KLTASPENYIRGRGKDDYLKKLPQEVKNVPMVVLVNSGSASASEIVAGALQDHRRAVV
                     MGQPTFGKGSVQTILPLGNNTAIKLTTARYYTPSGRSIQAKGIVPDIPLEDPVGEKTA
                     SLRLREADLAKHLLDVAKEKEQAEEKAKAEAAAVKYDFKPAPRPKELDEKAMRPEPGE
                     IVSKNDYELNQAVAFLKSRTLARAN"
     gene            2254..3000
                     /gene="moeB"
                     /locus_tag="VFY80_07710"
     CDS             2254..3000
                     /gene="moeB"
                     /locus_tag="VFY80_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX6007834.1"
                     /translation="MNDEQLLRYSRHILLPEIGIEGQERLLSARALVIGAGGLGSPVA
                     LYLASAGVGHITICDGDKVDLTNLQRQIVHRTHSIGRTKVDSARATLAEINPEARVVA
                     LAERVGGARLDELVDSADVVVDGCDNFATRHAVNRACVKFRKPLVSGAAVRFDGQVAV
                     FDLRDAHSPCYHCLFPESGENEDVRCAVMGVFAPLVGIVGSIQAAETLKVISGAGETL
                     VGRLLLVDALAMQTRIVRFAKDAACTVCAA"
     gene            complement(3009..3365)
                     /locus_tag="VFY80_07715"
     CDS             complement(3009..3365)
                     /locus_tag="VFY80_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007835.1"
                     /translation="MANVTTQQLAELLLGIARAQQAIIEAMENSKAGFKATHFRPTLE
                     AVSRIRSNRAESLADFPARLLLQLLGRSGPALDQVVADLEELLAREASPATPVEAGAT
                     AYAAGVDPATLNTQPT"
     gene            complement(3394..3981)
                     /gene="slmA"
                     /locus_tag="VFY80_07720"
     CDS             complement(3394..3981)
                     /gene="slmA"
                     /locus_tag="VFY80_07720"
                     /inference="COORDINATES: protein motif:HMM:NF007015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX6007836.1"
                     /translation="MGARTGERKTQILHTLAQMLESPSAERITTAALAARLEVSEAAL
                     YRHFTSKAQMFEGLIDFIENSLFGLINRITSEEKSALRQIDSIVGLLLGFAQKNRGMT
                     RVLTGDALVNEEERLQSRINQLHDRIEAALKQALRFGVTQQEFAAELDVTAQANLLMS
                     FVTGRWHQFAKSGFKRDPVEFWGKQWRQLLEGVMA"
     gene            complement(4024..4668)
                     /locus_tag="VFY80_07725"
     CDS             complement(4024..4668)
                     /locus_tag="VFY80_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX6007837.1"
                     /translation="MAWAKSWIFDLDNTLHNAAPHIFPHINRAMTAYLQTHLGLDEKA
                     AAELRRHYWTRYGATLLGLMRHHGTDPRHFLQDTHRFDDLERMLVCEPQLRHLITRLP
                     GRKFVFSNAPVHYSRAVLKGLCIADLFEGVYSIEDTRYRPKPDCYGFLRLTRANHLKP
                     QRCIMVEDTLSNLRTAKKLGMRTVWVSEAARAPGYVDVKISSLRQLPRMLTRLQ"
     gene            complement(4698..5585)
                     /gene="argB"
                     /locus_tag="VFY80_07730"
     CDS             complement(4698..5585)
                     /gene="argB"
                     /locus_tag="VFY80_07730"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX6007838.1"
                     /translation="MPLNTVSGAPKAKVLTEALPYIQRFHGKTIVVKYGGNAMIDVEL
                     KEDFARDVVLLKLVGMNPVVVHGGGPQIDDLLKRVGKKGEFVQGMRVTDAETMDVVEM
                     VLGGQVNKEIVNLINRHGGHAVGLTGKDGAFIRAKKMLVTAPGESDGKIDIGQVGEVD
                     AIDPRVIGTLLDGGFIPVIAPIGFGAKGESYNINADLVAGKVAEVLKAEKLVVLTNTP
                     GVLDKNGNLLTGLTARKVDELFADGTISGGMLPKISSALDAVKNGVNTCHIIDGRVPH
                     ALLLEILTNEGVGTLIRRR"
     gene            complement(5749..5910)
                     /locus_tag="VFY80_07735"
     CDS             complement(5749..5910)
                     /locus_tag="VFY80_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6007839.1"
                     /translation="MLRSERYYGKVYRAFTLEQPVDEATAQAKYENGVLEVTLPKKAA
                     VAAKRISVQ"
BASE COUNT         1058 a         1859 c         1938 g         1067 t
ORIGIN      
        1 ggtcgagcgg ctgcgtgaga cgctggcaca agttcgcgtg ctcgccgccg agatggagcg
       61 caacggggca gccctgaaat cgctcgctgt ggaagcgact tccgaaaagc gccgcctgga
      121 aggggaaaag gacgcacgca gccgcgttct tgcgcgcctc tcgcgcgaga tcgaccagca
      181 gcggcgcgag atcgggacgc tgaagcgcaa cgaaagccgg ctcgcccgat tgatcgaaga
      241 gctgggccgc gtggtggtac gcaggccctc ctcgccgcgc ctttcgaacc agcgcgtgcc
      301 gggccgtgcc gagccgagcg gccccttcga ggcgctcaag ggacggctga agcttccggt
      361 gcgcggggaa ctcgcgaacc gcttcggcag tccacggcgg gagggggcac tcacctggaa
      421 aggcttgttc atcaccgcgc gggctggtga tgaagtgcgg gccatcgcgg ccggcacggt
      481 cgtgttcgcc gactggctgc gcggcttcgg caatctcctc atcatcgatc acggcggtgc
      541 ctacatgagc ctctacggct ataaccaagc gcttctcaag cgggtgggtg aagccattcg
      601 tggcggcgac cccattgcca ccgtgggcaa tagcggcggt aacgcggatt cgggtttata
      661 cttcgagctg cggcatgagg gcaggccgct cgacccgctc aagtgggtcg atattcaatg
      721 agttgtaagc attcaagcag gggtgaagga gccagcaatg cgtaacagat tgcatcacat
      781 cggactgatc tttctcggca tcctcatggg tgtcgcgatc agtctgaatt tctcggccgg
      841 cgcacagaag gacgcggtgc tcgcgccgtt gccgatcgag gagctgcgtg ccttcaccga
      901 ggtcttcggc cgcgtgaaga gcgattacgt cgagccggtc gaggacaagc gcctcatcac
      961 tcaggcgatc aacggcatgc tgaccggcct cgacccgcac tcggcgtatc tcgatcagga
     1021 agcgttcagg gagcttcagg tcggcacgca aggcgaattc ggcgggctcg gcatcgaagt
     1081 cggcatggag gaagggttcg tcaaggtgat cgcaccgatc gacgacacgc cggccgcgcg
     1141 ctccggaatc aaggcgggcg atctcatcat caagctcgac gacgcgtcgg tcaaaggcat
     1201 gaccctcacc gacgcggtga agcgcatgcg cggcaagccc aacacccaga tcacgctcac
     1261 catcgtgcgc aagggcgagg acaagccgct cgtcgtgacc gtgacgcgcg cgatcatcaa
     1321 ggtgcagagc gtgaaagcga agctgctgga gccggggtac ggctatttcc gcgtgagcca
     1381 gttccaagag cacaccggcg agaatctggc cgcggcgatc acgcgcgtat tcaaggaaaa
     1441 ccaggggtcg atgaaaggca tcgtgctcga cttgcgcaac gatcccgggg ggctgctgaa
     1501 tggcgctgtc gcggtttctg cggcattcct cccgtcgggc tcgctcgtgg tgtacaccga
     1561 tggccgcacc gaggactcga agatgaagct cacggcctcg ccggagaact acatccgcgg
     1621 gcgcggcaag gacgactacc tgaagaagct gccgcaggag gtgaagaacg tgccgatggt
     1681 ggtgctcgtg aacagcggct cggcttcggc ttccgagatc gtcgcgggcg cgctgcagga
     1741 ccaccgccgg gcggtggtca tggggcagcc cacgttcggc aagggctcgg tgcagaccat
     1801 tctgccgctc gggaacaata cggcgatcaa gctcaccacc gcgcgctatt acacgccgag
     1861 cgggcgctcg atccaagcga aaggcatcgt gcccgatatt ccgctggagg atccggtggg
     1921 cgagaagacg gcgagcctga ggctgcgcga agcggatctc gccaagcacc tgctcgacgt
     1981 ggcgaaggag aaggagcagg ccgaagagaa agcgaaggcg gaggcggccg cggtcaagta
     2041 tgacttcaag cctgcgccgc gtcccaagga gttggacgag aaggcgatgc ggccggagcc
     2101 gggggagatc gtctcgaaga acgactacga actcaaccag gcggtcgcat tcctgaagag
     2161 ccgcacgctc gctcgggcaa actgacgacc gcggcggcgg ctgcggcctc tgaaaaaatg
     2221 cccggcttca ggccgggcat ttcatttgaa ccgatgaacg acgagcagct actgcgctac
     2281 agccggcaca ttctcctgcc ggagatcggg atcgaggggc aggaaaggct cctctccgcg
     2341 cgcgcgctcg tgatcggcgc ggggggactc ggctctccag ttgcgcttta cctcgcttcc
     2401 gccggagtgg gccacatcac gatctgcgac ggcgacaaag tcgacctcac gaatcttcag
     2461 cgccagatcg tgcaccgcac gcactcgatc ggccgcacca aggtggattc ggctcgcgcg
     2521 acgctggctg agatcaatcc cgaggcccgt gtcgtcgcgc ttgcagagcg cgtgggaggc
     2581 gcgcgcctcg acgagctggt cgattcagcg gacgtggtgg tggatggctg tgacaacttt
     2641 gccacgcgcc acgccgtcaa ccgggcgtgc gtgaaattcc gcaagccgct cgtgtccggc
     2701 gctgcagtca gattcgacgg gcaagtcgcg gtgttcgatc tgcgcgatgc gcactcgccg
     2761 tgctaccact gcctttttcc agagagtggc gaaaacgaag acgtgcgttg cgcggtgatg
     2821 ggcgtcttcg caccgctcgt gggcatcgtc ggttccattc aggcggcaga aacgctgaag
     2881 gtgatcagcg gtgcgggaga gacgctcgtc ggccggctgc ttctcgtgga tgcgctcgcc
     2941 atgcaaaccc gcatcgtgcg tttcgcaaaa gacgcggcct gcacggtctg cgccgcgtaa
     3001 gagaggcgct atgtcggctg cgtgttgagc gttgccgggt ccacgccggc ggcgtacgcc
     3061 gtcgcgccag cctccacagg ggtcgcaggc gatgcctcgc gcgcgagcag ctcctcgagg
     3121 tccgccacca cctgatcgag cgccggaccg ctgcggccca aaagctgcag cagcaggcgc
     3181 gccggaaagt cggcgagcga ttccgcgcgg ttgctgcgga tccgcgacac cgcttcgagc
     3241 gtgggccgga agtgcgtcgc cttgaatccc gccttcgaat tctccatcgc ctcgatgatc
     3301 gcctgctgcg cgcgtgcgat gccgagcagg agctccgcga gttgttgtgt cgtcacgtta
     3361 gccatgtctt ccccgtacga ttgtcgtatc gcgtcacgcc atgacgcctt ccaggagctg
     3421 gcgccactgc ttgccccaaa actccaccgg atcgcgcttg aagccgctct tcgcgaattg
     3481 atgccagcgc ccggtgacga agctcatcag cagattcgcc tgcgcggtga catccaactc
     3541 agcggcgaac tcctgctgcg tcacgccgaa acgcagcgcc tgcttgagcg cggcctcgat
     3601 gcggtcgtgc agctggttga tgcgcgactg cagccgctcc tcctcattca ccagcgcgtc
     3661 tccggtcagc acccgcgtca tgccacggtt cttctgcgcg aaaccgagga gcaggccgac
     3721 gatcgagtcg atctggcgga gcgcgctctt ctcctcgctc gtaatgcggt tgatgaggcc
     3781 gaagagcgaa ttctcgatga aatcgatcag cccttcgaac atctgggcct tgctcgtgaa
     3841 atggcgatag agggccgctt ccgagacctc cagccgggct gcgagggctg cggtcgtgat
     3901 ccgctccgcc gacgggctct ccagcatctg cgccagcgtg tgcagaatct gtgtttttcg
     3961 ctcccctgtc ctcgctccca ttccctgccc cggccggatc gcagcgatcc gctcgcgccg
     4021 gtctcattgt agccgggtca gcatccgggg caactgtctc aggctcgaaa tcttgacatc
     4081 aacatagccc ggcgcccggg cagcctcgct cacccatacc gtgcgcatcc cgagcttctt
     4141 ggctgtgcgc aggttggaaa gcgtgtcttc caccatgatg cagcgctggg gcttgaggtg
     4201 gttggcgcgc gtgagccgca gaaagccgta acaatcgggc ttcggccggt agcgcgtatc
     4261 ctcgatcgag tagacgcctt cgaaaagatc ggcgatgcag aggcctttca gcaccgcgcg
     4321 tgagtaatgc accggcgcgt tcgagaagac gaacttccgg ccgggaagcc gcgtaatgag
     4381 atggcgcaac tgcggttcgc acacgagcat gcgctcgaga tcatcgaagc ggtgcgtgtc
     4441 ctgcaggaag tgccgcggat cggtgccatg atggcgcatg agacccagaa gcgtcgcgcc
     4501 gtaacgcgtc cagtaatggc gccgcagttc cgcagccgcc ttctcgtcca gaccgaggtg
     4561 cgtctgcagg taggccgtca tcgcacggtt gatatgcgga aagatatggg gcgccgcgtt
     4621 gtgcagcgtg ttgtcgaggt cgaagatcca ggactttgcc caggccacgc ggcggccgtt
     4681 ttttcgatgc gcgcgcttca gcggcgccgg atgagcgtgc caacgccctc gttcgtcaga
     4741 atctcgagca gcagggcgtg cggcacacgg ccgtctatga tgtggcaggt attgacgccg
     4801 ttcttgaccg catcgagcgc cgagctgatc ttgggcagca tcccgccgga aatggtgccg
     4861 tcggcgaaga gctcgtccac cttgcgtgcc gtgagtccgg tgaggagatt gccgttcttg
     4921 tcgagcacgc ccggcgtatt ggtgaggacc accagctttt ccgccttgag cacctccgca
     4981 accttgcccg cgacgaggtc ggcgttgatg ttgtaggact cacctttcgc gccgaagccg
     5041 atcggcgcga tgaccggaat gaagccgccg tcgagtagtg tcccgatgac ccgcgggtcg
     5101 atcgcatcca cttcgcctac ctgcccgatg tcgatcttgc cgtccgattc gcccggcgcg
     5161 gtgacgagca tcttcttggc gcggatgaat gcgccgtcct tgccggtgag gccgacggcg
     5221 tgaccgccgt ggcggttgat gagattcacg atctccttgt tcacctgtcc gccgagcacc
     5281 atttcgacga cgtccatggt ttcggcatcc gtcacccgca tgccctgcac aaactcgccc
     5341 ttcttgccga cgcgtttgag gagatcgtcg atctgcggtc ccccgccgtg gacaacgacc
     5401 gggttcatgc cgacgagttt cagcagcacg acgtcacgcg cgaagtcttc cttcagctcg
     5461 acatcgatca tggcgttgcc gccatacttc acaacgatgg tcttgccgtg gaagcgctga
     5521 atgtagggca gcgcctccgt gagaaccttg gctttcggcg caccggatac ggtgttgagc
     5581 ggcatggatg tgcaggaatg tgagggcaca cttattgtac acgagcaaga agaaggcgag
     5641 aggcgctccc gcgctgtcgc cttcttctcc tcatgatgct cacgcacgcg cagatgagcg
     5701 tgcgcacgcc gctcagggcg ccccggcggc ggagccgccg gtccccgatc actggacgct
     5761 gatgcgtttc gccgcgactg ccgccttctt cggcaaagtc acttccagca cgccgttttc
     5821 gtacttggcc tgcgccgtgg cttcgtcgac gggctgttcg agagtgaagg cacggtagac
     5881 cttgccgtaa tagcgttcac ttctcagcag gcgctcgcca tt
//
LOCUS       DASURG010000441         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1098119, whole genome shotgun sequence.
ACCESSION   DASURG010000441 DASURG010000000
VERSION     DASURG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098119"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /gene="lnt"
                     /locus_tag="VFY80_07740"
     CDS             <1..883
                     /gene="lnt"
                     /locus_tag="VFY80_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX6007840.1"
                     /translation="GGWGLKHVEWTRPTGEAVSVSLLQGNVPQDLKFRPDRIQRTLEE
                     YRALAESSRSRLIVFPETALPLFLDNVPPAYLDGLAAHARRNGGDVLIGVPEREPDGR
                     YYNSVISLGTARPQRYHKFHLVPFGEFIPLRPVLADIVGALAIPLQDFSRGTAQPRPL
                     EVAGQRVAVNICYEDAFGEEIIRQLPDATLLVNVSNVAWFGRSIAPGQHLQISQARAL
                     ETGRYMLRATNTGVTAVIDPRGAVVAAAPQFTRAVLTHRVHGYEGATPYVRWGNVPVL
                     VICGAFGIFALALRARA"
     gene            954..1841
                     /gene="glyQ"
                     /locus_tag="VFY80_07745"
     CDS             954..1841
                     /gene="glyQ"
                     /locus_tag="VFY80_07745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX6007841.1"
                     /translation="MLTFQQLILTLNRYWDQHGCVLLQPYDMEMGAGTFHTATFLRAI
                     GPEPWNAAYVQPSRRPKDGRYAENPNRLQHYYQYQVVMKPSPREFQDLYLGSLTALGI
                     DPRAHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQQVGGLDCKPVLGELTY
                     GLERLAMYLQGKENVFDLVWVAGVTYRDVYHQNEVEQSRYNFELSNAELLFQHFAQFE
                     AEAKRLVESDLVLPAYEMVLKCSHTFNLLDARGAISVTERAAYIGRVRALARLIAQAY
                     YDAREQLGFPMLAKQEKAA"
     gene            1842..>2383
                     /locus_tag="VFY80_07750"
     CDS             1842..>2383
                     /locus_tag="VFY80_07750"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX6007842.1"
                     /translation="MEASLLVELLTEELPPKSLKTLGTVFGEEVLKGLERRKLRASGE
                     SALRIFATPRRLGALITGVLDKAADREQEVSGPSVKAGRDAAGKPTAALLGFARKIGI
                     AVEALEERDSPKGRVFVARLQVSGVALDAAIGEILTEAIRKLPIPKVMRWGDRDAEFV
                     RPVHGLVILHGKRVVPGSVLG"
BASE COUNT          432 a          751 c          770 g          430 t
ORIGIN      
        1 cggaggctgg ggactcaaac acgtcgagtg gacgcgcccg actggcgagg ccgtgagcgt
       61 aagcctgctg caggggaacg tgccacagga ccttaaattc cgccccgatc ggatccagag
      121 gaccctcgaa gagtaccgcg cgcttgctga atcgagccgg agccggctca tcgttttccc
      181 ggagaccgcg ttgccgctgt ttctcgataa cgtgccgccc gcctatctgg acggtctggc
      241 ggcgcatgcg cgaagaaacg ggggcgatgt gctgatcggt gtcccggaac gcgagcccga
      301 cggccgctac tacaacagcg tgatctcgct cgggacagcg cggccgcagc gctatcacaa
      361 gttccatctc gtgccgttcg gcgaattcat tccgctgcga cccgttttgg cggacatcgt
      421 gggcgcgctt gcgatcccgc tgcaggattt ttcgcgcggt acggctcagc cccgtccgct
      481 cgaggtggcc gggcaacgcg tcgcggtgaa catctgctac gaggacgcct tcggcgagga
      541 gatcatccgc cagttgcccg atgcaacgct gctcgtcaac gtgagcaacg tcgcgtggtt
      601 cgggcgttcg atcgcgccgg gacagcatct gcagatttcc caggcacgcg cgctcgagac
      661 cggccgctac atgctgcgcg ccacgaatac cggcgtaaca gccgtcatcg atccacgcgg
      721 cgcggtggtc gctgcggcgc cgcagttcac acgcgcagtg ctgacacacc gcgtgcacgg
      781 ctacgagggc gccacgccgt acgtgcgatg ggggaacgtt cccgtactcg taatatgcgg
      841 cgcgttcggc atcttcgctc ttgcattgcg cgcacgagct tgagcagcgc cgcccttacc
      901 ggcgtaccag caaaacccgc tacaatctgc cattttccgg cgacgccgac gcgatgctga
      961 cgtttcagca actgattctc acgctcaatc gttactggga tcagcacgga tgcgtgcttc
     1021 tgcagcccta cgacatggag atgggcgcag gcacgttcca tacggcgaca ttcctgcgcg
     1081 cgatcggacc cgagccgtgg aacgccgcct acgttcagcc ttcgcgccgt cccaaggacg
     1141 gacgctacgc cgagaatccg aaccgcctgc agcactacta tcagtaccag gtggtgatga
     1201 agccttcgcc acgcgaattc caggatctct atcttgggtc gctcacggca ctcggcatcg
     1261 atcccagagc gcatgacata cggttcgtgg aggacgactg ggagtctccg acgctcggcg
     1321 cgtggggtct cggctgggaa gtatggctag acgggatgga agtcacgcag ttcacttact
     1381 tccagcaggt cggcgggctg gactgcaagc cggtgctcgg ggaactcacc tacgggctcg
     1441 agcggcttgc gatgtatctg cagggcaagg aaaacgtgtt cgacctcgtg tgggtggccg
     1501 gcgtgacgta ccgcgacgtc tatcatcaga acgaagtgga gcagtcgcgt tacaacttcg
     1561 agctctctaa cgccgaactg cttttccagc acttcgcgca gttcgaggcg gaagcgaagc
     1621 gcctcgtcga gtcggatctc gtgcttccgg cgtacgaaat ggtcctcaag tgctcgcata
     1681 ccttcaatct gctcgatgcg cgcggcgcga tttcggtgac cgagcgcgca gcttacatcg
     1741 ggcgcgtgcg cgctctcgca aggctcatcg cgcaggcata ttacgatgcg cgggagcagc
     1801 tcggtttccc catgctcgcg aagcaggaga aagcagcgta gatggaagcg agcctcctcg
     1861 tcgagctgct gaccgaggaa cttcctccca agtcgctcaa gacgctcggc acggtgtttg
     1921 gcgaggaagt gctgaagggg ctggagcgcc gcaagttacg agcatccgga gagagcgctc
     1981 tgcgtatctt cgcgaccccg cggcgcctcg gtgccctcat caccggcgtg ctcgacaaag
     2041 cggcggaccg ggagcaggaa gtgagcgggc cgtcggtcaa ggcggggcgc gatgccgccg
     2101 gcaagccgac cgcggcgcta ctgggctttg cccgcaagat cggcattgcg gtcgaggcgc
     2161 tggaagagcg cgacagcccg aaaggacgtg tttttgtcgc gcgcttgcaa gtctccggtg
     2221 tcgcactcga cgcagcgatc ggcgaaatcc tcaccgaggc aatccggaag ctgcccattc
     2281 ccaaagtgat gcgttggggc gaccgcgatg ccgaattcgt gcgacccgtc cacggactcg
     2341 tcatcctgca cggaaagcgc gtggtcccag gttcggtcct ggg
//
LOCUS       DASURG010000442         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1098137, whole genome shotgun sequence.
ACCESSION   DASURG010000442 DASURG010000000
VERSION     DASURG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098137"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFY80_07755"
     CDS             complement(<1..797)
                     /locus_tag="VFY80_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007843.1"
                     /translation="MTPFGSDRPRRKLPIVLVLVGVFVAALAGVGYYFGPRLERDAPQ
                     ITLAPDAGVLGRAPMEIRVADPGSGLKAITVTLSAGGAEHTIASEQYADPVKEKTFAL
                     AASKLTGIKEGPATLRVTARDGSLWRFFGGNETVLQKELIIDITPPTVALIADDRYVT
                     FGGVGAIVYKPSSDTVTSGVRIGEYFFPGFQGPVKNQPDHFIALFAHPYNVPTSTRAM
                     LVATDKAGNTREMALVYELKGAKYRKSTIAVSESFIQGKVAPLLNDVA"
     gene            complement(941..2488)
                     /locus_tag="VFY80_07760"
     CDS             complement(941..2488)
                     /locus_tag="VFY80_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX6007844.1"
                     /translation="MNGAQSLVRTLVANGVNVCFTNPGTSEMHFVAALDKVEGMRCVL
                     GLFEGVVTGAADGYYRMTGRPACTLLHLGPGLGNGLANLHNAKKARSGIVNIVGEHAT
                     YHLKHDAPLTADIAGIARPVSHWIHTATSARTLAMDCASAIQQARMPPGQIATLIAPA
                     DTAWGEAEAPARAESPPARKMVARDAVSGAARALTDGAPAMLLLGGAALRGRALEDAG
                     RIAAKTGCRLMSEYNNARMEGGAGRVKVDRLPYPVDQAFATLKGVRRLVLAGAKVPVS
                     FFAYPGKPSVLTPEDCVVTKLADAEDDLAGALSSLVAALDATRLSPAHVNDLARPALP
                     SGKVTPDGIACTLAALLPDDAIVIDESVTTGRNLGTTMASAPPHDWLNIMGGSIGWAL
                     PAATGAAIGAPERKVVALEGDGSGMYTLQALWTMARENLDVTVVVLANRSYQILHTEL
                     RNMGAGTPGQRAADMLTLDRPHLDWVSLARGLGVEGGRATTLDEFAGHLASAFSRRGP
                     YLVELVF"
     gene            2630..3610
                     /locus_tag="VFY80_07765"
     CDS             2630..3610
                     /locus_tag="VFY80_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007845.1"
                     /translation="MKIEARLCLAIALAVAGSTAFAQSWAPQKNIEIVAGSAPGGSND
                     KTARAMERILSEYKLVPTTITVINRPGGGGSIAYTYVAQRAGDAHYLAIGSSNLLSNN
                     LVGSSTLHHHDFTPIAQLYEDYAVFAVQTESPLKVGKDLADRLRKDPRTVVVGFANTF
                     GGSRHVAAGLLVKAVGSSVRDLKPVVYKGSAEAITSVLGGHIEVVVVGATNAAGHVAN
                     GRMRVIAVAAPKRFSSGPLATAPTWREQGIDVVYGNWRAIYAPKGLTPAQVRYWEATL
                     RKMSDTAEWKTDLEKNYWTPVFVVGDALRTEIEREQAHLKTILAELGLAK"
     gene            3615..>4136
                     /locus_tag="VFY80_07770"
     CDS             3615..>4136
                     /locus_tag="VFY80_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007846.1"
                     /translation="MKSRWILAFVVCLAGQAFAAQGYPVRPVRLVIPYPPGGPTDFVG
                     RLVGQKLSPLLKQQVVVDNRPGAGTIIGSEIVARAAPDGYTLLFGTGGGTFLAPLMLP
                     KVPYDPHRDFAPVAMLVQSPQVLVVHPSVAAKSVSELIALAKAKPGVLNFASVGTGTS
                     PHLGGELFQALTGA"
BASE COUNT          724 a         1351 c         1344 g          717 t
ORIGIN      
        1 gcaacatcgt tcaacagggg cgcaaccttg ccctgaatga agctctcgga cacggcaatg
       61 gtgctcttcc tgtatttcgc acccttaagt tcgtagacga gcgccatctc ccgggtattc
      121 cccgccttgt cggtggcgac cagcatggcc ctggtgctgg tgggcacgtt gtagggatgc
      181 gcgaaaagag cgatgaagtg atccggctga ttttttacgg gcccctggaa acccgggaag
      241 aagtattcgc cgatgcggac gccgctcgtt accgtatctg acgaaggctt gtagacgatc
      301 gccccgacgc cgccaaaggt cacatagcgg tcatcggcga tgagggcgac ggtgggcggc
      361 gtgatgtcaa tgatgagctc cttctgcagc acggtctcgt tgccaccaaa gaatctccac
      421 agcgagccat cccgcgccgt cacgcgaagg gtcgcggggc cttccttgat gccggtcagc
      481 ttggaagcgg cgagcgcgaa tgtcttctct ttgacgggat cggcgtactg ctcggacgcg
      541 atcgtatgct cggcgccccc cgctgaaagc gtgacggtaa tggctttcaa tcccgagccc
      601 gggtcggcaa cccgaatctc catcggcgca cgcccgagca ccccggcgtc cggtgcgagc
      661 gtgatttgcg gcgcgtcccg ctcgagccgc gggccgaagt aatagccgac ccccgcaagc
      721 gcggcgacga agactccaac aaggacaaga acgatgggaa gttttctgcg aggtctgtcg
      781 gatccgaaag gggtcatctt gtttcagggg agtttagtgg ctgaaaagtt ttttgcgcac
      841 gtcggcgtat ctcgtcctgc gcaagtgtag cctcagaata gcagcgatcc aagccgcaca
      901 gcggtgaaat aagccgcagc tgcagcgaaa gtcgcgcttt tcagaatacg agttcgacga
      961 gatacggccc gcggcgcgag aatgcgctcg ccaggtgccc cgcaaattcg tccagcgtcg
     1021 tcgcgcgtcc gccttccacg ccgagtccgc gcgcgagcga aacccagtcg agatgcgggc
     1081 gatcgagcgt cagcatgtcg gccgcccgct gtccgggcgt gccggccccc atgttgcgca
     1141 attcggtgtg caggatctgg tacgatcggt tcgcgagaac cacgacggtc acatcgagat
     1201 tctctcgcgc catcgtccac aacgcctgca gtgtgtacat gccgctgccg tcgccctcca
     1261 gcgcgacgac cttgcgctcc ggcgcgccga tcgcggctcc ggtcgccgcc ggcagcgccc
     1321 agccgataga gccgcccatg atgttgagcc agtcgtgcgg cggagcgctc gccatggttg
     1381 tgccgagatt cctgcccgtc gtcaccgatt cgtcgataac gatcgcatcg tcgggaagca
     1441 gcgcagccag cgtgcaggca atgccgtccg gcgtcacctt gccggacggg agggcgggac
     1501 gcgcgagatc gttcacgtgc gccggggaaa gccgcgtcgc atccagcgcc gccacgagcg
     1561 acgagagcgc acccgcgaga tcgtcttcgg cgtccgccaa cttcgtcacc acgcaatctt
     1621 cgggcgtaag cacgctcggc ttgccgggat aggcgaagaa cgatacgggc actttggcgc
     1681 cggcgagcac gaggcgccgc acgcccttca gcgttgcgaa cgcctgatcg accggataag
     1741 gcaaccggtc caccttgacc cgccccgccc cgccttccat gcgcgcgttg ttgtattcgg
     1801 acatgagacg gcagccggtc ttcgcggcga tgcggcccgc gtcctccagc gcgcgcccgc
     1861 gcagcgcggc gccgccaagc agcagcatcg cgggcgctcc gtccgtcagc gcgcgcgctg
     1921 caccgctgac cgcatcgcgc gccaccatct tgcgcgcggg tggtgattcc gcgcgcgccg
     1981 gcgcttcggc ctcgccccaa gcagtgtcgg ccggtgcgat gagcgtcgcg atctgccctg
     2041 gcggcatgcg cgcctgctgg atcgcggatg cgcaatccat ggcgagggtg cgcgcgctgg
     2101 tcgccgtatg aatccagtgc gaaaccggtc gcgcgatgcc cgcgatgtcg gccgtgagcg
     2161 gcgcatcgtg cttgagatgg taggtcgcgt gctcgccgac gatattgacg atgccggagc
     2221 gggccttctt cgcgttgtgc aggttcgcga gcccgttgcc gagcccgggg ccgagatgga
     2281 gcagcgtgca cgcgggccgg ccggtcatgc ggtaataccc gtccgctgcg ccggtcacca
     2341 cgccctcgaa gagcccgagc acgcagcgca tgccttccac cttgtcgagc gcagcgacga
     2401 agtgcatttc ggacgttccg gggttggtga aacagacatt cacgccgttc gcgacgagcg
     2461 tgcggacgag gctttgcgcg ccgttcatgg ctcgaacccc acacatggat ttgggcaagg
     2521 ttaccgcggc gctgcgccat cgggcaatgg cgaccccaac ttggcgaggc gcgtacggga
     2581 gactatgatg cgcaggccat tgcggacacc ctcgggagga ggatcacgca tgaagattga
     2641 agcacgcctt tgcctggcaa tcgcgctcgc cgtcgccggt agcaccgctt tcgcccaaag
     2701 ttgggcgccg cagaaaaaca tcgaaatcgt tgcgggctcc gcgcccggag gcagcaacga
     2761 caagaccgcg cgcgccatgg agcgcatctt gagcgagtac aagctcgtgc ccaccacgat
     2821 cactgtcatc aaccgcccgg ggggaggagg cagcatcgcg tacacctacg tcgcgcagcg
     2881 cgccggcgat gcccactacc tcgccatcgg cagcagcaac ctcctcagca acaacctcgt
     2941 cggttccagc acactccacc atcacgattt cacgccgatc gcgcagctct atgaggacta
     3001 tgcggtcttt gccgtgcaga cggagtctcc tctcaaggtc ggcaaggatc tcgcggatcg
     3061 cctgcgcaag gacccgcgca ctgtggtagt cggctttgcc aataccttcg ggggtagccg
     3121 tcatgtcgct gccggcctgc ttgtgaaagc cgtcggcagc agcgtgcgag acctgaaacc
     3181 ggtcgtatac aagggatcgg cggaggccat cacctcggtg ctgggcgggc atatcgaagt
     3241 agtcgtggtc ggagcgacca atgccgccgg tcacgtcgcg aatggacgca tgcgggtcat
     3301 cgcggttgcg gcgcccaagc ggttttccag cgggccgctg gccactgcac ccacctggcg
     3361 cgaacagggc atcgacgtgg tctatggcaa ctggcgtgcg atctacgcgc ccaaaggtct
     3421 caccccggcg caggttcgct actgggaggc gacattgcgc aagatgtccg acactgcgga
     3481 gtggaaaacg gatctcgaaa aaaactactg gacgccggta ttcgtcgtgg gcgacgcgct
     3541 gcgcacagag atcgagcgcg agcaggcgca tctcaagacc attctcgccg aactcgggct
     3601 cgccaagtaa tatcatgaag tcgcgctgga ttctcgcgtt cgtcgtttgc ctggccgggc
     3661 aggcgtttgc ggcgcagggc tatccggtga ggccggtgcg gctcgtgatt ccctacccgc
     3721 ccgggggccc gaccgatttc gtcggtcgcc tggtggggca gaagctctcg ccgctcctga
     3781 agcagcaggt cgtcgtcgac aatcgcccgg gcgccgggac catcatcggc agcgaaatcg
     3841 tggcccgtgc cgcacccgat ggctacacgc tgctcttcgg caccggtggc ggcacgtttc
     3901 tcgcgccgct catgctgccg aaggtaccgt acgatccgca tcgagatttc gcaccggtcg
     3961 cgatgctggt gcaaagtccg caggtgctgg tcgtccatcc gtcggtcgcg gctaaaagcg
     4021 tgagcgaatt gatcgcgctc gccaaagcta agcccggtgt gctcaatttc gcgtcggtcg
     4081 gcaccggcac ctccccgcat ctcggaggcg agctgttcca ggcgctgacc ggagcg
//
LOCUS       DASURG010000443         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_179448, whole genome shotgun sequence.
ACCESSION   DASURG010000443 DASURG010000000
VERSION     DASURG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179448"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFY80_07775"
     CDS             <1..252
                     /locus_tag="VFY80_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007847.1"
                     /translation="IYGSQMMTQQERDQYQQRMRTATTAEERERIRAEHHARMQERAK
                     QRGMSLPDQPPAAGPRGGMGPGSGYGPGSGGGYGGGRGR"
     gene            complement(288..539)
                     /locus_tag="VFY80_07780"
     CDS             complement(288..539)
                     /locus_tag="VFY80_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007848.1"
                     /translation="MIGVSDLDLNRLRQFLDEEERFARAVPQGDRRHAVARKMLALIA
                     DARAKLKPSADGTANYVPVKSIIEDLAALRARMETRESS"
     gene            complement(536..>2329)
                     /locus_tag="VFY80_07785"
     CDS             complement(536..>2329)
                     /locus_tag="VFY80_07785"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6007849.1"
                     /translation="KLTLESQRLVGNALEPRACLVAYDAGSGRYALHVPLQGAGTMRA
                     QILQVTGLEKERITLVAQDVGGSFGVRGAAYPEYFAAMMAAKRLARPVKWVGSRSETF
                     MSDFHGRALSLTGEIAMDEDGKFLAIRFDDRADLGAYGGAFGSFIATRNMTVTAGGVY
                     RIPAIYARTRLAYTNTVPVSAYRGAGRPDIAYAIERLVDHAAHEHGFDRIELRRRNLI
                     PRDAMPYQTANAGTYDSGDFEAVMDDALQRADWTGFPARRGDSARAGKLRGIGIATYL
                     EAGGGGAAPKDQVAAEFDPDGRMTLYAVTQSSGQGHETIYPQIVAATLGIDASEIRFH
                     PSPPAADLIGNGTGGSRGVLGTGSAFRVLGQKLIDVARAHAAQALGVAEGELRYSEGR
                     YHAGDRSIGFVELARRLAGPKPHPLDTIAEGVFGATYPNGCHIAEVEIDPETGAATIA
                     RYTAVDDLGNVINPTLVEGQVHGGVMQGAGQVFGEHAVYDPATGQLLSGSFSDYVMPR
                     AGWLRHIEVHDHPVPTPTNALGAKGVGEAGCSGSLPALANAVIDALRPLGVTHLDMPF
                     TPARLWHAIRSAPRRLEEIPSFRRNPESGEA"
BASE COUNT          374 a          794 c          766 g          395 t
ORIGIN      
        1 atttacggct cgcagatgat gacccagcag gagcgcgatc agtatcagca gcgcatgcgc
       61 accgcaacga ccgccgagga acgcgagcgg atccgcgcgg aacatcacgc gcgaatgcag
      121 gagcgtgcca agcaaagggg catgtcgcta ccggatcagc ctcccgcagc tggaccgcgt
      181 ggcggcatgg ggccgggaag tggctacgga ccgggcagcg gcggcggcta cggcggcgga
      241 cgcggacgct gatcgcctga tgtgttgcga ctccgtgcgc cgggggctta gctcgattcg
      301 cgcgtttcca tccgcgcgcg cagtgcggcg agatcctcga taatcgattt caccggcacg
      361 taattcgcgg taccgtctgc ggacggcttg agcttggcgc gcgcgtccgc aatgagcgcg
      421 agcatttttc tcgcaacggc gtggcggcga tcgccttggg ggacggcacg cgcaaatctc
      481 tcttcctcgt cgagaaactg ccgcaagcgg ttgagatcga ggtcggacac gccgatcacg
      541 cctctccgga ttccgggttg cgccggaatg acggtatttc ttcgaggcgt cgcggcgcgc
      601 tgcggatcgc atgccataga cgcgcgggcg tgaacggcat gtcgagatgc gtcacgccaa
      661 gcggacgcaa tgcatcgatc accgcattgg cgagcgccgg aagcgagccg ctgcagcccg
      721 cctctcccac gcctttcgcg ccgagcgcgt tggtcggggt cggcacggga tgatcgtgca
      781 cctcgatgtg tctcaaccat cccgcacgcg gcatcacgta gtcgctgaag cttccgctga
      841 gaagctggcc cgtcgccggg tcatacaccg cgtgctcgcc gaaaacctgc ccggcgccct
      901 gcatgacccc gccgtgcacc tgcccttcga ccagcgtcgg gttgatcacg tttccgagat
      961 cgtccaccgc agtgtagcgc gcgattgttg cggcaccggt ctccgggtcg atttccactt
     1021 cagcgatatg acagccgttg ggatacgttg cgccgaagac gccctccgcg atcgtgtcga
     1081 gcgggtgcgg cttcgggccc gcgagcctgc gggcgagctc cacgaagccg atcgaacggt
     1141 cgccggcatg gtaacgcccc tcgctatagc gcagctcgcc ttctgccacg ccgagcgcct
     1201 gcgcggcatg cgcgcgcgcg acatcgatca gcttctgtcc cagtacccga aacgcgctgc
     1261 cggttccgag cacgccgcgc gagccgccgg tgccgttgcc gatcaagtcg gcggcgggcg
     1321 ggctcggatg aaaccgtatc tccgatgcgt cgatgccgag cgtcgctgcc acgatctgcg
     1381 ggtaaatcgt ctcgtgaccc tgtcccgacg actgcgtgac ggcatagagc gtcatccggc
     1441 cgtcgggatc gaattccgcc gcgacctgat ccttgggcgc cgcaccgccg cccccggctt
     1501 cgagataagt cgcgattccg atgccgcgaa gcttgcccgc gcgcgctgag tccccgcgtc
     1561 gcgccgggaa gcccgtccag tccgcgcgtt gcagcgcatc gtccatcacc gcttcgaagt
     1621 cgcccgagtc gtaggtccct gcgttcgccg tctggtacgg catcgcatcg cgcgggatca
     1681 ggttcctgcg ccgaagttcg atgcgatcga agccgtgctc gtgcgccgca tggtccacca
     1741 gccgctcgat ggcatacgcg atgtccgggc gtcccgcacc gcgataggcc gataccggaa
     1801 ccgtattggt gtaagcgaga cgcgtccgtg cgtagatcgc gggaatgcgg tagacgccgc
     1861 ccgcggtgac ggtcatgttg cgggtcgcga tgaacgagcc gaacgcgccg ccgtaagcgc
     1921 cgagatccgc gcgatcgtcg aagcggatcg cgagaaactt cccgtcttcg tccatggcaa
     1981 tctcgcccgt gagcgagagc gcgcgcccgt gaaagtcgct catgaaggtt tcgctgcggc
     2041 ttcccaccca tttcaccggg cgcgcgagcc gctttgcggc catcatcgcg gcgaagtact
     2101 cgggatacgc ggcgccccgc acgccgaagc tcccgccgac gtcctgcgcg acgagggtga
     2161 ttctctcctt ctctagaccg gtcacctgca gaatctgggc gcgcatggta cctgcaccct
     2221 gcagcggcac gtggagcgca tagcgaccgc tcccggcgtc gtacgcgacg aggcacgcgc
     2281 gcggctcgag cgcattgccg acgagcctct ggctctccaa cgtgagctt
//
LOCUS       DASURG010000444         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_894041, whole genome shotgun sequence.
ACCESSION   DASURG010000444 DASURG010000000
VERSION     DASURG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894041"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..759
                     /gene="nth"
                     /locus_tag="VFY80_07790"
     CDS             127..759
                     /gene="nth"
                     /locus_tag="VFY80_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6007850.1"
                     /translation="MNRNKIGEIFRRLRAANPAPRSELEFSCPFELLIAVILSAQATD
                     RSVNLATRELFREANTPQTFLQLGQAGLERYIKRIGLYRTKAKNILAACRLLVEEHGG
                     AVPRTREALERLPGVGRKTASVILNTAFGEPTIAVDTHIFRVANRTGLAPGRNVLEVE
                     RKLMKTVPDEFRRDAHHWLILHGRYVCRARKPDCGRCIINDLCEYRDKVL"
     gene            759..1190
                     /locus_tag="VFY80_07795"
     CDS             759..1190
                     /locus_tag="VFY80_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEX6007851.1"
                     /translation="MFAPTREESRHFFFDTWEKYRRGEPLQGLERVALQVIVLHPEYH
                     AILEAPERYADRDYRPESGEMNPFLHLALHVAVEEQLAIDQPRGIAAAYQRLLGKTGS
                     EHDAKHLVLECLGETLWRAQREGTPPDETLYIECLERQARS"
     gene            complement(1197..1514)
                     /locus_tag="VFY80_07800"
     CDS             complement(1197..1514)
                     /locus_tag="VFY80_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6007852.1"
                     /translation="MKHSALLLYVTVLLAHPAWASDTVAGEKKSKPCEACHGPAGNSA
                     SPDFPRLAGQNYDYLVKTLNDYKSGARKNPIMAPQAANLSKRDISDLAAYYSSQRGLY
                     VKY"
     gene            complement(1525..1869)
                     /locus_tag="VFY80_07805"
     CDS             complement(1525..1869)
                     /locus_tag="VFY80_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6007853.1"
                     /translation="MRHPFIKLFTAAALFGAAGALAAAEGNAAAGAEKNSMCAGCHGI
                     PGYKTAFPEVYSVPKLGGQHAAYIVKALQAYKTGERSHPSMRAISATLSEQDIADLAA
                     YYASPQSQSAAK"
     gene            1964..3811
                     /locus_tag="VFY80_07810"
     CDS             1964..3811
                     /locus_tag="VFY80_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6007854.1"
                     /translation="MLRHTNVLDTVNSQAPARFSPWLTKLLPFLRWRALLTRETLRAD
                     ALAGLVGAAIVLPQGVAFATLAGMPPQYGLYAAMVPAVIAALFGSSWHLVSGPTNVIS
                     IVIFASLSPVAEPGSADYIRLVLTLTFLVGVMQLAMGLARMGILVNFVSHTVIIGFTA
                     GAACLIFTAQLGNFFGLDIARGASFPETLAHFVSNLDDVNPYVTAVGAATLLTGIAAR
                     RYLAQFPYMIVAMVVGTVLALALDALFGREATRIATVGALPAALPTLSTPDLSPTTLK
                     NTLPVALAVTLLALTEAVSIARALALKSGQRIDANQEFIGQGLSNIAGSFFSAYASSG
                     SFNRSGANQEAGARTPLAAIFSVPILILILFAVGPLVAYLPLAVMGAILFLVAYGLFD
                     LHHIRNILRTSRQETVVLLATFIAAIFADLEFAIYAGVLLSLMLYLTRTSRPMILDVK
                     PDPAPDSYHYTADSGLPDCPQLKMVRVNGSIFFGAVDHVQRELEEIDELNPRQKHVLI
                     VASGINFIDVAGAEMLAAEARRRRRLGGGLYFYRLKDTPRALLERGGYMKDIGWENVF
                     PVKTRAVAAIYPKLDTEICRNCRALIFQECRVALPNGEPRVARSNHRKE"
     gene            complement(3819..4883)
                     /locus_tag="VFY80_07815"
     CDS             complement(3819..4883)
                     /locus_tag="VFY80_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6007855.1"
                     /translation="MEPVMAEFADLTWGGVLALLLIGFVGGLVSGFIGSGGAFVLTPA
                     MMSLGVPGAVAVASNICHKFPKALVGAMKRHRFGHVDLKLGLVMAASATVGVWVGADI
                     QQRIKMNFGEAGSNLYVSLAFMVVLAIVGGYVLYDALHIRRNGGVPLEEQRSRLACWI
                     QSIEIPGTMMHFQTANVRVSALFTIPLGFATGVLAGTIAVGGFIGVPGMIYILGAPAL
                     IASATELVIAFVMGLAGTVKYALHGLVDIRLALILLAGSLFGVQLGAIGTTYVHPYVI
                     KVVMAAIMLIVALSRALVIPVYMVELDMISLASPVMSALRSASLFFLVLALGIGAFII
                     LGAMLRGHRQVALDAGAPAK"
     gene            complement(4890..5120)
                     /locus_tag="VFY80_07820"
     CDS             complement(4890..5120)
                     /locus_tag="VFY80_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007856.1"
                     /translation="MEAHKPYLGLICSGVGSGSLYALLYAYEAEVMVYFTRTDGLYPA
                     LPILAAFVFSLVHGAFAGFFWEVIGIRARRRS"
BASE COUNT          995 a         1714 c         1628 g          920 t
ORIGIN      
        1 agtgcgcgtg cggccgccca ggagacgcgc aagcggcaaa cgatacaaaa agcgctcgcg
       61 cgggcccgcg cccgactcgg ccagcgcagc cgctgagcct gagcccgcga acacaccgcc
      121 tgccacatga atcgcaacaa gatcggcgaa atcttcaggc gcctgcgcgc tgccaacccc
      181 gcgccgcgca gcgagctcga gttcagctgc cctttcgagc tgctgatcgc ggtgatcctc
      241 tcggcgcagg cgacggacag gagcgtaaac cttgccaccc gggagctgtt ccgcgaggcg
      301 aacaccccgc aaaccttcct gcagctcggg caggcaggcc tcgagcgcta catcaagcga
      361 atcgggcttt atcgcaccaa ggccaagaac atcctggcgg cgtgcaggct gctggtggag
      421 gagcacggcg gtgccgtacc gcggacccgc gaagcgctgg agcgactccc gggcgtcggc
      481 cgcaagacgg caagcgtcat cctgaacacg gccttcgggg aaccgaccat cgccgtggac
      541 acccatatct ttcgggtggc gaaccgcacc ggcctcgccc ccggccgcaa tgtgctggaa
      601 gtggaacgaa aactgatgaa gaccgtgccc gatgaatttc gccgcgacgc gcatcactgg
      661 ctgatactgc atggccgtta cgtgtgccga gcgcgcaagc ccgattgcgg ccgctgcatc
      721 atcaacgatc tgtgcgaata ccgcgacaag gtgctgtgat gttcgccccg acgcgcgagg
      781 aatcgaggca ctttttcttc gatacctggg aaaaataccg ccgcggcgag ccgctacagg
      841 gcctggagcg cgtcgcgctg caggtgatcg tgcttcaccc ggaatatcac gcgatcctgg
      901 aagcgcccga gcgctatgcc gaccgcgatt atcgccccga gtccggagag atgaatccgt
      961 tcctgcacct cgccttgcac gtggctgtcg aagaacagct cgcaatcgat cagccgcgcg
     1021 gcatcgccgc tgcataccaa cgactgctgg gaaagacggg atcggagcac gatgccaagc
     1081 atctcgtgct cgagtgcctt ggggagacgc tctggcgcgc ccagcgcgag ggcacgccgc
     1141 ccgacgaaac gctctatatc gagtgcctcg agcgccaggc ccgaagctga tccggctcag
     1201 tacttgacgt aaagcccgcg ctggctcgaa tagtacgcgg cgagatcgga gatatctcgc
     1261 ttcgagagat tcgccgcctg cggcgccatg attggattct tccgcgcgcc ggatttgtag
     1321 tcgttgagcg tcttcacgag gtagtcatag ttctggcccg ccagcctggg aaaatcgggt
     1381 gacgcgctgt ttcccgcggg accatgacat gcttcgcatg gtttcgattt tttctcgccc
     1441 gcgacggtat ctgaagccca tgcgggatgg gcgagcagca cggtcacgta gagaagcaat
     1501 gcagaatgtt tcatcgcggc tccctcactt cgctgcagac tgcgactgcg gcgaagcata
     1561 gtatgccgca aggtccgcaa tgtcctgctc cgacagcgtc gcggagatcg cacgcatcga
     1621 aggatggctg cgctcccccg tcttgtaagc ctgcagcgcc tttacgatat acgcggcatg
     1681 ctggccgccg agcttgggca cgctgtacac ctcggggaac gccgtcttgt agcctgggat
     1741 gccgtgacag cccgcgcaca tggaattctt ctccgcgccg gcggcggcat tgccttccgc
     1801 tgctgcgagc gcgccggctg cgccgaaaag cgcggctgcg gtaaagagct tgatgaatgg
     1861 atgtctcatg cggttttttc tcggtagcaa aacgggtttt ttgcgggcgc tagtataccg
     1921 cggcccgcag cgttgcggca ccgtgaacga tgcccgataa tgcatgctgc ggcacaccaa
     1981 cgtgctcgac acagtgaatt cgcaagcccc cgcgcgcttt tctccctggc tgaccaagct
     2041 ccttccgttt ctgcgctggc gcgcgctcct cacgcgtgaa acgctgcgcg ccgatgcgct
     2101 ggcgggtctc gtgggcgctg caatcgtgct gccccagggc gtcgcattcg caacgctggc
     2161 cggcatgcca ccgcagtacg gcctctatgc cgccatggtt ccggccgtca tcgccgcgct
     2221 gttcggctct tcatggcatc tcgtctccgg gccgaccaac gtcatctcga tcgttatctt
     2281 cgccagcctg agcccggtgg cagagccggg cagcgccgat tacatccggc tagtgctaac
     2341 gctcactttc ctcgtcggcg tgatgcaact ggcgatgggg ctcgctcgca tggggatact
     2401 ggtcaacttc gtgtcgcata cggtgatcat cggtttcacc gcgggcgccg cctgcctcat
     2461 cttcacggcg cagctcggga acttcttcgg gctcgacatc gcgcgcggcg cgtccttccc
     2521 ggaaacgctg gctcacttcg tatcgaatct cgatgacgtc aatccgtacg tgacggcggt
     2581 cggcgcggcg acactgctca ccgggatcgc cgcacgccgt tatctggcgc agtttcccta
     2641 catgatcgtc gcgatggtcg tcggcacggt gctcgcgctt gcgctcgatg cgctgttcgg
     2701 ccgtgaagcg acccgcatcg cgacggtcgg ggcactgccg gcggccctgc cgacactgtc
     2761 cacgcccgat ctctcgccca caacgctcaa gaatactctg ccggtcgcgc tggccgtcac
     2821 gctgctcgcg ctcaccgagg cggtgtcgat tgcgcgcgcg ctcgcgctga aatcaggaca
     2881 gcgcatcgac gccaaccagg aattcatcgg gcagggactg tcgaatatcg caggaagctt
     2941 cttctccgct tacgcgagca gcggctcctt caatcgcagc ggcgccaatc aggaagcagg
     3001 tgcccgcacg ccgctcgccg cgatattttc ggtgccgata ctgatcctca tcctgttcgc
     3061 ggtagggccg ctcgtcgcgt atctcccgct cgcagtgatg ggcgcgatcc tcttcctggt
     3121 tgcgtatggc ctcttcgacc ttcatcacat ccgcaacata ttgcgcacga gccggcagga
     3181 gacggtggtc ctgctcgcaa ccttcatcgc ggcaatcttc gcggatctcg agttcgccat
     3241 ctatgcgggc gtgctgctgt cgctcatgct ctatctcacg cgcacctcgc gcccgatgat
     3301 cctcgacgtc aaacccgatc ccgcaccgga cagctatcac tacaccgcgg acagcggcct
     3361 ccctgactgc ccgcagctca agatggtgcg cgtcaacgga tcgatttttt tcggcgcagt
     3421 ggatcacgtt cagcgcgagc tcgaggagat cgacgagctg aacccccgcc agaagcacgt
     3481 gctgattgtc gcgagcggca tcaacttcat cgacgtcgcg ggcgcggaga tgctcgccgc
     3541 ggaggcgcga cgccggcgcc ggctgggggg cgggctttac ttttatcggc tgaaggacac
     3601 gccgcgcgca ctgctcgaaa ggggcggcta catgaaggat atcggctggg aaaacgtctt
     3661 tcccgtgaag acgcgcgccg ttgcggcgat ctaccccaag ctcgatacgg agatctgccg
     3721 caactgccgg gcgctcatct tccaggagtg tcgggtggca ctgccgaacg gcgagccccg
     3781 ggtggcgcgt tcaaaccatc ggaaggagtg accgtccgct atttcgccgg cgccccggca
     3841 tcgagcgcga cctggcggtg gccgcgcagc atggcaccga ggatgatgaa ggcaccgatt
     3901 ccgagtgcca ggacgaggaa gaacaggctt gccgaccgca gcgcggacat gaccggagag
     3961 gcaagcgaga tcatgtcgag ctcaaccatg tagaccggaa tcacgagcgc gcggctcaag
     4021 gcgacgatca gcatgattgc cgccatgacg accttgatca catagggatg aacataagtc
     4081 gttccgatcg cgcccaactg cacgccgaag agcgatcctg caagcagaat gagcgccagg
     4141 cggatgtcca ccagcccgtg cagcgcatac ttgacggtcc cggccagccc catgacgaag
     4201 gcgatgacga gctccgtggc gctcgcgatc aaggccggcg cgccgagaat gtaaatcatc
     4261 cccgggacgc cgataaagcc gcctaccgca atcgtgccgg cgagaacgcc ggtcgcgaaa
     4321 ccgagcggaa tcgtgaataa agcggacaca cgcacgtttg cggtctggaa gtgcatcatc
     4381 gtgcccggaa tctcgatgga ttgaatccag cacgcgaggc gcgagcgttg ctcttccaga
     4441 ggcacgccac cgttgcggcg gatgtgaagc gcgtcgtaca ggacataacc gcccacgatg
     4501 gcgagaacga ccatgaaggc aaggctcacg tagaggttcg aaccggcctc gccgaaattc
     4561 atcttgatgc gttgctggat atcagcgccc acccatacgc caaccgtggc ggaggctgcc
     4621 atcacgaggc cgagcttcag gtccacgtgg ccgaaccggt ggcgcttcat cgcgccgacc
     4681 agcgctttag ggaatttgtg gcagatattg ctcgccaccg ccacggcgcc cggcacgccg
     4741 aggctcatca tggccggcgt cagcacgaat gcgccaccgg atccgatgaa tccgctgacc
     4801 agcccgccga cgaaaccgat cagcagaagc gcgagcacgc caccccacgt gagatcggcg
     4861 aactccgcca tgacgggttc catcgcgctc tagcttctcc gtcgcgcgcg gatgccgatt
     4921 acttcccaga aaaagccggc aaaagccccg tgcacgagcg agaaaacgaa agcggcgagg
     4981 atcggaagcg ccggatagag gccgtcggtc cgcgtaaagt agaccatgac ttcggcctcg
     5041 taggcgtaga ggagcgcgta gaggctgccg gagcccacgc cggagcagat caatcccagg
     5101 tagggcttgt gcgcttccat gatggcgcga ttattctagc agcatgacgc cgcgcgttcc
     5161 ggcgcagggt gaagagtaac gggttgcagg gttagaatcg gaagcatggc ctaccgactg
     5221 tcccgcatct ctacccgcac gggagacgac ggcacca
//
LOCUS       DASURG010000445         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1405949, whole genome shotgun sequence.
ACCESSION   DASURG010000445 DASURG010000000
VERSION     DASURG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405949"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFY80_07825"
     CDS             <1..369
                     /locus_tag="VFY80_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007857.1"
                     /translation="EVKDVTWLRPDGVEMTPADWNNAHAKSLTILLSGEAGLTHLTAR
                     GEQESDDTFLMMMNASEQDVVFRLPPQPGTRWTVLIDSSDGVDKRPDRTHRAGRRIPV
                     KARSMLLFVRALPREPRPAE"
     gene            366..2204
                     /gene="treZ"
                     /locus_tag="VFY80_07830"
     CDS             366..2204
                     /gene="treZ"
                     /locus_tag="VFY80_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX6007858.1"
                     /translation="MRRRHTMPFGAVLDGMGARFRLWAPTAERVELMLLSEIGRETLL
                     MDAADAGWFEARVGYARVGDRYRFRVDGGTEVPDPASRHQPEDVHGLSALIDPESYRW
                     HDDAWHGVPWHEVVLYELHIGTFTREGTFAAARERLPYLRDLGITAVELMPIADFPGA
                     RNWGYDAVLPFAPDSVYGHPEDLKRFIDAAHGVGLAVFLDVVYNHFGPEGNYLHVYAR
                     EFFTARHETPWGAAINFDGAGSRTVRDFYVHNALYWLEEYHFDGLRLDAVHAICDESP
                     THILTEIAQAVRRGPGRGRCVHLVLENDDNAACFLERAQNGARGYDAQWNDDAHHALH
                     VLVTGETDGYYSDYADAPVRHLARTLAEGYAYQGERSHYRGRPRGETSRHLPATAFID
                     FLQNHDQVGNRAFGDRISTLASAEALRAAYALLLLAPAVPLLFMGEEFGCTQPFPFFC
                     DFGGELGQAVTEGRRRECAAFARFSAPRATEAIPDPNASSTFESAVIDWDALRAPQHA
                     AWHARFAHLLALRRKAIVPLLPRLVPGSGRYTLPGSRALRIEWHLVEGARLVVVANLG
                     DAIVKASREAVGELIYAEPAQPIFSSGIEDLAPWSVTWFLDRGSGA"
     gene            2201..>3916
                     /gene="malQ"
                     /locus_tag="VFY80_07835"
     CDS             2201..>3916
                     /gene="malQ"
                     /locus_tag="VFY80_07835"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX6007859.1"
                     /translation="MNLALLDRLCAICGIHTAYRDVWGAERRVPPETRIALLAAMGVS
                     AASDAELERSVEDCESEEWRRPLPPVVVFRGASGPLHVPLRIPTRLAHTQFECTLVTE
                     NGERTTLHCRAEALRRTADRTVDGEPFAAFDLLVNELPACGYHRLELASREASALQSV
                     ATIIVAPPHCYLPEAVQQGRLWGPVVNLYGIRSRRNWGIGDFTDLGTLVEFAAAAGAG
                     AVGVNPLHALYPHDPEHASPYSPSSRLQLNALYLDPERVAEFPDCRAARERVESRAFQ
                     DALQSLRAADLLDYSAVSRLKTEVLGMLYQSFREQHLDRGTGRSRAFRAFQSTGGETL
                     RRASVFHALQEALQARDPSAWGWRSWPQAYRDPGSPEVERFAAEHRDRIEYYDYLQWN
                     ADEQLGAAGQRALELHLGVGIYLDLAVGADPGGAETWGQHALYAESVTIGCPPDDFGP
                     NGQDWGMPPLVPHRLRETAYAPFAALLRANMRHAGALRIDHIMGLMRQFWVPAGMGPG
                     AGGYVSFPVDDLLGVLALESERNRCMVIAEDLGTLPDGLAERLQDQRILSYRLLYFQR
                     ESDGGF"
BASE COUNT          636 a         1279 c         1310 g          691 t
ORIGIN      
        1 gaagtgaagg acgtgacgtg gctgcgcccg gacggcgtcg agatgacgcc ggccgactgg
       61 aacaacgcac atgccaagtc gctcacgatc ctgctttccg gagaggcggg cttgacgcac
      121 cttaccgcgc gcggcgagca ggaatcggac gacacctttc tcatgatgat gaatgcgtcc
      181 gaacaggacg ttgtttttcg gcttccgccg cagcccggca cgcgctggac ggtattgatc
      241 gattccagcg acggcgtcga caaacggccc gatcgcacgc accgcgcggg caggcgcata
      301 ccggtgaagg cgcgctcgat gctgctcttc gtgcgcgcgt tgccgcgtga gccccgccct
      361 gccgaatgag gcgccgccat acgatgccgt tcggtgccgt gctcgatggc atgggcgcgc
      421 gcttccgctt gtgggctccg accgcggagc gagtggaact catgctgctg tcggagatcg
      481 gtcgcgaaac cctcctcatg gatgcggcgg atgccgggtg gttcgaagcg cgggtcggat
      541 acgcacgcgt cggcgaccgc taccgcttcc gcgtcgacgg cggcaccgag gttccggatc
      601 ccgcttcgcg ccatcagccc gaggacgtgc acggactgag tgcgctcatc gatcctgaaa
      661 gctatcgatg gcatgacgac gcatggcacg gcgtgccgtg gcacgaagtc gtgctgtacg
      721 agctgcacat cggcacgttc acgcgtgagg gcacgtttgc ggcggctcga gagcggctac
      781 cctacttgcg cgatctcggc atcacggcgg tcgaactcat gccgatcgcc gattttcctg
      841 gcgcacgcaa ctggggttat gacgcggtac tgccgtttgc gccggacagt gtctacggtc
      901 acccggagga tctgaagcgt ttcatcgacg cggcgcacgg agtgggactc gcagtatttc
      961 tcgacgtggt ctacaaccat ttcggcccgg agggcaacta tctacacgtc tacgcgcgcg
     1021 agtttttcac ggcgcgccat gaaacgccgt ggggcgcagc catcaatttc gacggcgcgg
     1081 gcagtcgcac ggtacgcgat ttctacgttc acaacgcgct ctactggctc gaagaatatc
     1141 acttcgacgg gctgcggctc gacgcggtgc acgcgatctg cgacgaatcg ccgactcaca
     1201 tcctgaccga aatcgcgcag gccgtgcgcc gcggtcccgg tcgcggacgc tgcgtgcacc
     1261 tcgtcctcga aaacgacgac aacgccgcct gcttcctgga gagggcgcag aacggtgctc
     1321 gcgggtacga cgcgcagtgg aacgatgatg cgcaccatgc gctgcacgtg ctcgtcaccg
     1381 gtgaaaccga cggctattac tcggactacg ccgatgcgcc ggtgcgccat ctcgcgcgca
     1441 cgctcgcaga gggatatgct tatcagggcg aacgctcgca ctaccgcgga aggccccgtg
     1501 gcgagacctc gcgccacctg cccgccacgg cgttcatcga cttcctccag aaccacgacc
     1561 aggtcggcaa ccgggcgttc ggagaccgca tcagcacgct ggccagtgca gaggcgctac
     1621 gggcagcgta tgcgctgctg ctcctggcgc ccgctgtgcc gctgctcttc atgggcgaag
     1681 agttcgggtg cactcagcct tttccgtttt tctgcgactt cggcggcgaa ttggggcaag
     1741 cggtcaccga aggacgacgc cgggaatgtg cagcgttcgc acgcttcagc gctccgcgcg
     1801 ccaccgaggc gatacctgat cccaacgcgt catcgacatt cgagagcgca gtcatcgatt
     1861 gggatgcgct gcgcgcgccg cagcacgccg catggcacgc tcgcttcgct catctgctcg
     1921 cgctgcgccg taaggcgatc gttcccctcc tgccccgatt ggtaccgggc agcggcaggt
     1981 atacgttgcc tggatcccgc gcgctgcgca tcgaatggca tctcgtcgag ggcgcgcggc
     2041 tggtggtcgt cgcgaatctc ggagatgcca tagtcaaggc gagccgcgag gcggtcggcg
     2101 aactgatcta cgccgagccg gcccaaccga tcttttcctc gggaatcgag gacctggcgc
     2161 cgtggtcggt aacgtggttt ctcgaccgcg gaagcggcgc atgaatctcg ccttactcga
     2221 ccggctttgc gcgatctgcg gcatccacac cgcctaccgc gacgtgtggg gcgccgaacg
     2281 ccgggtcccg ccggagacgc ggattgcgct gcttgccgct atgggcgtga gcgccgcaag
     2341 cgacgcggaa ctcgagcgca gcgtcgaaga ttgcgagtcc gaggaatggc gccgtccgct
     2401 gcccccggtg gtcgtctttc gtggagcaag cgggccgctc cacgtcccgc ttcgcattcc
     2461 gacgcgtctg gcgcacacgc agtttgaatg cacgctcgtg acggaaaacg gcgaacggac
     2521 aaccctgcat tgcagggccg aagcgctgcg gcgcacggcc gacagaacag tggacggtga
     2581 gccgtttgcg gcattcgatc tcctggtgaa cgaattgccc gcctgcggtt atcaccggct
     2641 ggaactcgct tcgcgcgaag catccgcgct ccagtccgtt gcgacgatca tcgttgcgcc
     2701 accgcactgc tacctgccgg aggccgtgca gcaagggcgt ctctggggac cggtggtgaa
     2761 tctttacggc atacgctcgc gtcgcaactg gggcatcggc gatttcacgg acctgggcac
     2821 cctggtggag ttcgccgctg ctgcgggcgc gggcgcggtg ggtgtgaacc cgttgcatgc
     2881 cctttatccg cacgatcccg agcacgcgag cccgtacagc ccgtcgagtc gcctgcagct
     2941 gaatgcgctc tacctcgatc ccgagcgcgt cgcggaattt cccgactgcc gtgcggcgcg
     3001 ggagcgggtc gaaagccgcg cgttccagga tgcactgcaa tcgctccgcg ccgccgatct
     3061 cttggactac tccgcggtct cgcgactcaa aaccgaagtg ctgggaatgc tctaccagag
     3121 ctttcgcgag cagcacctcg accgcggcac cggacgcagc cgcgccttcc gggcgtttca
     3181 gagtacgggc ggcgagacgc tgcgccgcgc ctcggtcttc cacgcgctcc aggaagcgtt
     3241 gcaggcgcgc gatccatccg catggggatg gcgcagctgg ccgcaggcct atcgtgaccc
     3301 cgggtctccg gaggtcgaac gcttcgccgc ggagcatcgt gatcgcatcg agtattacga
     3361 ctacctgcag tggaatgcag acgagcaact gggcgcagcg ggacagcggg cgctcgagct
     3421 ccacctcggt gtaggcatct atctcgacct cgcggtgggc gctgatccgg gcggtgctga
     3481 aacgtggggg cagcacgcgt tgtacgcgga gagcgtgacc atcgggtgcc cgcccgacga
     3541 cttcggtccg aacgggcagg actggggcat gccgccgctc gtgccgcatc ggctgcgtga
     3601 gaccgcgtat gcgccgtttg cagcgctcct tcgggcgaac atgcggcatg caggggcgct
     3661 gcgcatcgac cacatcatgg gtctcatgcg gcaattctgg gtgccggcgg gcatggggcc
     3721 gggcgccggc ggttacgtct cttttccggt ggacgatctg ctgggcgtgc tggcgctcga
     3781 aagcgagcgc aaccgctgca tggtgatcgc ggaggatctc ggcacgctgc cggacgggct
     3841 cgccgagcgc ctgcaggacc agcgtattct ttcttaccgg ctgctgtact tccagcgcga
     3901 gtcggacggt gggttc
//
LOCUS       DASURG010000446         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_485632, whole genome shotgun sequence.
ACCESSION   DASURG010000446 DASURG010000000
VERSION     DASURG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485632"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFY80_07840"
     CDS             <1..262
                     /locus_tag="VFY80_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6007860.1"
                     /translation="AGAERARMPGGQSRWSAGKDLSWEPLRIERVCEVKYDHLQGDRF
                     RHAALFLRWRPDKPPAECRYDQLAVTEPYALGKVFGSRHAQT"
     gene            complement(207..1265)
                     /gene="ligD"
                     /locus_tag="VFY80_07845"
     CDS             complement(207..1265)
                     /gene="ligD"
                     /locus_tag="VFY80_07845"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6007861.1"
                     /translation="MAAIREELTAGTRRIAVSNPRKILFPQAGYTKIDLARYYLAVAE
                     GVLRGSGGRPNMLVRYPDGVEGEFFYQKRAPVPRPDWIEVVTIRFPSGRSADEVVPRD
                     AAALVWLANLGCLELHPHPVRAEDLDHPDELRIDLDPVPGVKWDQVREVGALVHEVLQ
                     DFKLTGWPKTSGKRGLHVYVRIKPHWTYDEVRRAALALAREVERRAPSIATTRWWKEE
                     RRGVFLDYNQNARDRTIAGAYSVRPTRDATVSAPIKWRELASCEPRDFTLVTVPVRFA
                     KLGDPHRAIDRRACSLEPLLELAARQERAGQGEAPWPPHYRKARGEPPRVQPSRRRAA
                     VTSARGANRKPSRARRAP"
     gene            1488..1715
                     /locus_tag="VFY80_07850"
     CDS             1488..1715
                     /locus_tag="VFY80_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007862.1"
                     /translation="MTERKRNIQGEGDKESDRKYRERTTEFVKSKHGQEEIERAGQLS
                     EEEARRLKQYEDQGKSRAKGEDPQIKHQSKR"
     gene            complement(1775..2176)
                     /locus_tag="VFY80_07855"
     CDS             complement(1775..2176)
                     /locus_tag="VFY80_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007863.1"
                     /translation="MLVAAVVLWFLGLVTLVPYGTYYLFYEAPRDQYALLITLVLFWI
                     FGYWGVVGPLLAALKVRAVFRAIEAARSSDDLVRTLKSSDARDVAIDFIATENRIPRF
                     LASWVYRVLVSRLVKGARGAADRPASRRDGD"
BASE COUNT          465 a          770 c          791 g          398 t
ORIGIN      
        1 tgccggcgcc gagcgcgcgc gcatgccggg cggacagagc cgctggagcg ctggcaagga
       61 cctctcgtgg gagccgcttc gtatcgaacg ggtgtgcgaa gtgaaatacg accatcttca
      121 gggcgaccgc ttccggcatg cggcgctttt tctgcgctgg cggccggaca agccgccggc
      181 cgaatgccgt tacgaccagc tcgcggttac ggagccctac gcgctcggga aggttttcgg
      241 ttcgcgccac gcgcagacgt gaccgccgcg cggcgacgcg agggctgaac gcgaggcggt
      301 tccccgcgcg ccttgcggta atgcggcggc catggcgcct caccctgtcc tgcgcgctcc
      361 tggcgcgctg caagctccag cagcggttcg agagaacagg cgcgccggtc gatcgcgcgg
      421 tgcgggtcgc ccaactttgc gaagcgcacc ggcacagtca ctagcgtgaa atcgcggggc
      481 tcgcaagaag caagctcgcg ccacttgatc ggcgccgaga ccgtcgcgtc ccgcgtgggc
      541 cgcacggaat aggcgccggc gatggtgcgg tcgcgcgcat tctggttgta gtcgagaaag
      601 acgccgcggc gctcttcctt ccaccatctc gtcgtggcga tactcggcgc gcggcgctcg
      661 acttcgcgag ccaacgcaag cgctgcacgc cgcacctcat cgtatgtcca gtgcggcttg
      721 atgcgcacgt agacgtgcag tccgcgcttg cccgatgttt taggccaccc ggtgagcttg
      781 aagtcctgaa gcacctcgtg cacgagcgcg cccacttcgc gcacctgatc ccatttcacg
      841 ccgggaaccg gatcgagatc gatgcgaagc tcgtcgggat ggtcgaggtc ctcggcgcgc
      901 acaggatgcg ggtgaagctc caggcaaccc aagttggcga gccacacgag cgccgccgca
      961 tcccgcggca ccacttcgtc ggcgctgcgg ccggacggaa aacggatcgt gacgacttcg
     1021 atccaatcgg gacgcgggac gggcgcgcgc ttctggtaga agaattcgcc ttcgacgccg
     1081 tcgggatagc gcaccagcat gttgggccgt ccacccgatc cccgcaaaac gccttcggca
     1141 acggcgagat agtagcgcgc gagatcgatt ttggtgtagc ccgcctgggg aaacaggatc
     1201 ttgcgcgggt tcgataccgc gatccgccgc gtgccagcgg taagctcctc gcggatagcc
     1261 gccatggatg ccttctcttc gctgtgtgtc gggtttggac atcacgcgac ttcggtgagt
     1321 gccgttagca tcatggcggg ctgtagtcgg cggacgacat ctcggcggtt gaagcgcgct
     1381 cgacgcgtga tgccccggaa ctttcaattg cctggtgcca ccaatcgggt accgccgacg
     1441 cgcatcacgc aatggcgaca tggcattcac aaaggatgga gacttccatg actgaacgga
     1501 aacgcaatat tcagggcgaa ggcgacaagg aatccgaccg gaaatatcgc gagcgcacga
     1561 ccgagttcgt caagtcgaag cacggccagg aggaaattga gcgggccgga caactgagcg
     1621 aggaagaagc gcggcgcctc aagcaatacg aggaccaggg caagtcccgc gcgaaaggcg
     1681 aagacccgca gatcaagcat cagtcgaagc gctgacgtgt gagccgccgc tcgtcgctgc
     1741 gatgcgatgt attatcgcgc cgagcgtcgg acgcttaatc gccgtcacgc cggcttgcag
     1801 gtcgatctgc cgcgccccgg gctcctttga ccagccgcga tacgagcacc cggtataccc
     1861 aggaggccag gaaccgcggg atgcgatttt ccgttgcaat gaaatcgatg gcgacatccc
     1921 gggcgtccga gctcttaagc gtgcgcacga gatcgtcgct cgaacgagcg gcctctatgg
     1981 cgcgaaacac ggcgcgcacc ttgagcgctg cgagtagcgg gccgacgacg ccccaatagc
     2041 cgaaaatcca gaagagcacg agcgtgatca atagtgcgta ctggtcgcgc ggcgcctcgt
     2101 aaaacaggta gtaggtgccg tagggcacga gcgtgacgag gccgaggaac caaagcacta
     2161 ccgctgcgac gagcaagttg tcacgccctc agtgccacga gatcacgccc agcggtttac
     2221 ggccaacact tggcatcacc cagcacggat ttccaggcag cacttttcga atgtcagggg
     2281 ctactcccct gctcgtgcgt agctctgggt gagcaattcc cagacatggc cgtccggctc
     2341 gctccagtag acgatgcgtc ccccgacgcg gctattgacc ttgaagtcag gctctccgtg
     2401 cggcaggctg cgataggcga tacc
//
LOCUS       DASURG010000447         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_77884, whole genome shotgun sequence.
ACCESSION   DASURG010000447 DASURG010000000
VERSION     DASURG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77884"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..209
                     /gene="rrf"
                     /locus_tag="VFY80_07860"
     rRNA            96..209
                     /gene="rrf"
                     /locus_tag="VFY80_07860"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            283..942
                     /locus_tag="VFY80_07865"
     CDS             283..942
                     /locus_tag="VFY80_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007864.1"
                     /translation="MKRAAMFFLLAVTAAFAEAQSWIAFTPAERDFRVLFPEPPSQST
                     AADGSTVFVATVARNEFDVKYAVYRLAPGIRLSGDPRADLQQRLEARARNEERGIRYV
                     HDDGGEPGWERYVFREGRVISVHRLVGRVGRYYELEVSMPRGSPELAIHTARDFFNSF
                     QATGVGLPSLATAVQQIDAWCRDRKDSFSQGFCRYSVCLQPGYEKYPHCTALAGLKNL
                     F"
     gene            complement(955..>3052)
                     /locus_tag="VFY80_07870"
     CDS             complement(955..>3052)
                     /locus_tag="VFY80_07870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX6007865.1"
                     /translation="AVCYSLGITEVDPARSSMLFERFMSRERNEPPDIDVDFEHQRRE
                     EVIQHVYRKYGRDRAALAATVITYRPRSALRDVGKALALDPIEVDRLAKSLAWWDGGR
                     VVPDRLREAGIDTASPVIRNLLMLVNDLVGFPRHLSQHVGGFVISRGALSHLVPIENA
                     AMPERSVIQWDKDDLETLGLLKVDVLALGILSAIRRALQFVNRHCGSALTMREIVDRG
                     DDPAVYRMIQKADTIGVFQIESRAQMSMLPRLKPGKFYDLVIEIAIVRPGPIQGGMVH
                     PYLRHRRSAEPVSYPSEAIRQVLERTMGVPIFQEQVMQLAMVAADFTPGEADALRRSM
                     AAWRRKGGLEKFQKKLKAGMANNGYSSEFSEQLYKQVLGFGEYGFPESHSASFALLAY
                     VSSWLKYYEPAAFLAAMLNSQPMGFYSPSELVQDARRHGVGVRPVDVALSEWECTLEP
                     ASCTCPAGGAKPNQPAVRLGLLMVKGLTKTAAERIIAMRAQKAFSDVDDLVRRAHPGR
                     RDLQCLAAAGALVSLAGHRRNAYWLTAPRDRPAHLLKDAPLAEATPLLAPPSEGEEIV
                     ADYTSLGLTLGRHPLELLRCRLDRMRLLPACDLRKTPDGTRARTAGIVTCRQRPGTAN
                     GVVFVTLEDETGYTNVVVWNDLVERQRRELLGSRLLGVEGRIQRDGDIVHLVAHRLFD
                     HTRLLGKLTTVARDFH"
BASE COUNT          582 a          907 c          977 g          586 t
ORIGIN      
        1 gcgctgcgaa ggtgatcagg ctcgtgtgaa tacaatcgaa tcgacttctt ccggttttgg
       61 tttgcaactt gagcgttgcg aattgacaag ctatgtctga cgactatagc gagttggaac
      121 cacgccttcc cattccgaac aggaccgtga aacgactcag cgccgatgat agtgcggatt
      181 gcccgcgcga aagtaggaca tcgtcaggca cccattcaag aaaggcccgg ccagcgatgg
      241 ccgggccttt tttatggcct gtgcactcgt tagggaggtg agatgaaacg agcggctatg
      301 ttttttctgc ttgcggtgac cgcggctttc gcggaagcgc agagctggat tgcgttcacg
      361 ccagcggaac gcgacttccg tgtgctcttt ccggagccgc cctcgcagtc gacggctgcc
      421 gacgggtcca ccgtattcgt ggccacggtc gcccgcaacg agttcgatgt gaagtacgcg
      481 gtgtaccgat tggcgccggg catacgcctt tccggggatc cacgcgccga tctccagcag
      541 cggctggagg cgcgcgcgcg gaacgaagag cgcgggatca ggtatgtgca cgatgacggc
      601 ggcgagcccg gctgggagcg ctacgtgttt cgcgagggca gagtaatcag tgtgcacagg
      661 ctcgtggggc gggtaggccg atattatgag ttggaagtca gcatgccgcg cggatcgccc
      721 gagcttgcaa tccatacggc acgcgacttc ttcaattcgt tccaggcgac cggtgtaggc
      781 ctcccttcgc tagccacggc ggtgcagcag atcgatgcct ggtgcaggga tcgcaaggat
      841 tcgttctcgc agggcttttg ccgctacagc gtgtgcctgc aacccggcta cgagaaatat
      901 ccccattgca cggctctggc aggtctcaag aatttatttt gagatgattg gtggttaatg
      961 aaaatcacgc gcgacagtcg tgagctttcc gagcagccgg gtgtgatcga aaaggcgatg
     1021 tgcaacgaga tgcacgatgt cgccgtcgcg ctggattctt ccctccacgc ccagcaggcg
     1081 tgagcctagg agctcgcgcc gctgcctttc gacgaggtcg ttccagacta cgacgttggt
     1141 gtaaccggtt tcgtcttcga gcgtgacgaa tacgacgccg tttgccgttc cgggacgctg
     1201 gcggcaagtg acgattcccg ccgtgcgcgc gcgcgttccg tccggggttt tcctgagatc
     1261 acaggcaggc aaaaggcgca tgcgatcgag tcggcatcgt aagagctcga ggggatggcg
     1321 gcccaacgta agtccgaggc tcgtgtagtc tgcgactatc tcctcacctt cggaaggcgg
     1381 ggcaagcaaa ggggtcgctt cggcaagcgg cgcgtccttc aacaggtgag caggccggtc
     1441 gcgaggtgca gtgagccagt acgcgttgcg acgatgtccg gcaagcgaga caagcgcgcc
     1501 agcagctgcc agacactgca gatcccgccg gcctggatgc gcgcggcgca cgagatcgtc
     1561 cacatcggaa aaggcctttt gtgctcgcat tgcgataata cgttcggccg cggtcttggt
     1621 cagccccttc accatgagca atccgaggcg cacggcaggc tgattcggct tcgctccacc
     1681 cgcagggcac gtgcatgagg cgggctccag cgtgcattcc cactcgctca gtgccacatc
     1741 caccggtcgc accccgacgc cgtggcggcg cgcgtcctgc acgagttccg aaggggaata
     1801 gaaccccatc ggctggctat tgagcatcgc ggcgaggaat gcggccggct cgtagtattt
     1861 gagccacgag gagacatacg cgagcagcgc gaagctcgcc gaatgcgatt caggaaatcc
     1921 atactcgccg aaacccagca cctgcttgta gagctgctca gaaaattcgg acgagtagcc
     1981 gttattcgcc attccggcct tgagcttctt ctgaaatttc tcgaggccgc ctttgcgccg
     2041 ccaggcggcc attgagcgcc gcaatgcatc cgcttcgccc ggcgtgaagt ctgcggcgac
     2101 catggcgagc tgcatgactt gctcctggaa aatcggcacg cccatcgtgc gctcgagcac
     2161 ctgacggatg gcttcggagg gatagctcac cggttccgct gaccgccggt ggcgcagata
     2221 cggatgcacc atgccgccct ggatcggtcc gggccgcacg atggcgattt cgattacaag
     2281 atcgtaaaac ttcccgggct tgagccgggg cagcatggac atctgcgcgc gcgattcgat
     2341 ctgaaagacg ccgatcgtgt ccgctttttg aatcatcctg tatacggcgg ggtcgtcgcc
     2401 gcgatcgacg atctcccgca tggtgagtgc gctgccgcaa tggcggttca cgaattgcag
     2461 cgcacgacgg atggccgaca gtatgccgag cgccagcacg tccactttca ggaggccgag
     2521 ggtctcgaga tcgtccttgt cccactggat cacgctgcgc tcgggcatgg cggcgttttc
     2581 gatcggcacg agatgcgaga gcgccccgcg cgaaattacg aaaccgccga cgtgctgcga
     2641 aagatgccgc gggaagccga cgagatcatt taccagcatg aggaggttcc ggatcaccgg
     2701 gctcgcggta tcgatgccgg cttcacgcag ccgatccgga acgacccgtc cgccatccca
     2761 ccacgcgagc gattttgcaa ggcgatcgac ttcgatcgga tcgagcgcga gcgccttgcc
     2821 gacgtcacgt agcgcgctgc gcggccggta ggtaatgacg gtagcggcaa gggcggcacg
     2881 atcccggccg tatttgcgat agacatgctg gatgacctcc tcgcggcgct gatgctcgaa
     2941 atcgacatcg atatcgggcg gttcattgcg ctcgcgcgac atgaagcgct cgaagagcat
     3001 gctcgagcgc gcgggatcga cttcggtgat cccgaggctg tagcacaccg cc
//
LOCUS       DASURG010000448         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_997850, whole genome shotgun sequence.
ACCESSION   DASURG010000448 DASURG010000000
VERSION     DASURG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997850"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFY80_07875"
     CDS             <1..732
                     /locus_tag="VFY80_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6007866.1"
                     /translation="LVLIYGESGTGKEFIVRMIPDQSPRATGPFVSINCAALTETLLE
                     SELFGHVRGAFTGAVRDKAGLFEVASNGTLFLDEIGEVAPTVQAKLLRALQEREIRRV
                     GAERTIKVNARVVAATNRDLRAAVAAGSFREDLYFRLGGFVISVPPLRDRRGDIPLLV
                     HDFVRRAAQRVKKDVQAVSPEAMTALMNYSWPGNVRELEHAIERAVIVARGETIKPRE
                     LPPEITQKPPQQVSHDSLDLHEQER"
     gene            1288..>2115
                     /locus_tag="VFY80_07880"
     CDS             1288..>2115
                     /locus_tag="VFY80_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX6007867.1"
                     /translation="MIHSPRDALTTQPEPHAAPHTDPPNRIRALQTFGQSIWLDYLQR
                     SLFTSGEFRRLIAEDDLRGATSNPSIFERAFADNTDYLDALQDIERRGDLEPLALYEA
                     LAIRDIRDAADLLRPVYDRTARADGYVSMEVSPYLAHDTAATIDEARRLWETIARENV
                     MIKVPASIEGLPAIRELTSEGINVNITLLFGIARYGAVAQAYLDGLSEFIQRGGDPGR
                     VSSVASFFVSRIDTMVDEMITQRLADATNPDLRASLTSLLGRVGIANARLAYQHYLAM
                     "
BASE COUNT          409 a          667 c          642 g          397 t
ORIGIN      
        1 ctcgtcctga tctacggaga gagcggcacc gggaaggaat tcatcgtgcg gatgatcccc
       61 gatcagagcc ctcgcgccac cgggccgttc gtgtcgatca actgcgccgc gctgaccgaa
      121 acgttgctgg agtcggagct ctttggacat gtccgtggag ccttcacagg tgccgttcgg
      181 gacaaggccg gcctgttcga ggtcgcgagc aacggaacgc tgtttctgga cgaaatcggg
      241 gaagttgcgc cgaccgtcca ggcgaagctg ctccgtgccc tccaggagcg cgaaatccgg
      301 cgcgtcggcg ccgaacgcac gatcaaggtc aacgccagag tcgtggccgc gacgaatcgc
      361 gatctgcgag cggcggttgc cgcaggcagc ttccgcgaag acctctattt ccggctcggt
      421 gggttcgtga tctcggttcc gccactgcgc gaccgccgtg gggacattcc gctgctcgtc
      481 cacgacttcg tgcggcgtgc ggcgcagcga gtcaagaagg atgtgcaagc ggtatcgccc
      541 gaggccatga ccgccctcat gaattacagc tggcccggca acgttcgtga gctcgagcat
      601 gcgattgagc gtgccgtcat cgtcgctcgc ggagagacca tcaagcctcg cgagctgcct
      661 ccggaaatca cacagaagcc gccacagcag gtctcgcatg acagtctgga tctgcacgaa
      721 caggaacggt gatgattgag cgtgcactcg aacgttttcg cggcaacagg aaggatgcgg
      781 ccaccgcgct gaagatcagc agtgcaaccc tctggcgcaa aatgaagcac tacggtctct
      841 tcggaccacg cacaagctct ggtgatcagg actggtaact ggcgatacgc gcatcgtgcg
      901 cgaattcccg cgcatcgtcg atcgtcgccc gatttcacat tgatgaagaa cccttcattt
      961 tgaatgacac gcgccttcca atgtctcggg acgcgctgcc gacccctcta acccctcagt
     1021 tcacttgcgt tgagcacagc ggcggccggg tggtactcga tttcgtgcct gattgaggca
     1081 tcgtccgagc gttcggacgc cgtggcgctc tcaactcaag tgagttatgg gcttagaaag
     1141 accagcaaac caccccacga cctcgcacat ccatctgaat cacgcgcggc ccggctcttg
     1201 cctctaatgc gtgagggcgc ctgatcccgg gtcgcctctt cgccgctggg ccctctcagt
     1261 cgatggcagt ctcagtggag ctcactcatg attcattccc cacgcgatgc gctgacgacg
     1321 caaccggaac ctcacgcagc gccgcacact gacccgccga atcgcattcg agccctgcag
     1381 acgtttggcc agtcgatctg gctggactac ctgcagcgaa gcttgttcac gagcggtgaa
     1441 ttcagacggc tgattgctga agatgacctt cgcggggcta cctcgaatcc ctccattttc
     1501 gaaagggcct tcgccgacaa cacggattac ctcgacgcgc ttcaggacat cgagcggcgt
     1561 ggcgatctgg agccactggc gctgtacgag gcgctcgcga ttcgcgatat ccgcgacgca
     1621 gcggacctgc ttcgcccggt ctatgatcgc accgctcgcg cggacggcta cgtcagcatg
     1681 gaagtgtccc cgtacctggc gcatgacacg gcggcgacga tcgacgaagc gaggcgcctc
     1741 tgggagacca tcgctcgcga gaacgtcatg atcaaggtgc cggcgtccat cgaaggactc
     1801 cccgcgattc gtgagctgac gagcgaaggc atcaacgtca acatcacgtt gctctttggc
     1861 atcgcgcgtt acggcgccgt ggcgcaggca tatctcgacg gtctgtcgga gttcatacaa
     1921 aggggcgggg atcctgggcg cgtgagcagt gtcgccagtt tcttcgtgag ccgcatcgac
     1981 acgatggtgg acgagatgat cacgcagcgc ctggccgacg cgaccaatcc ggatctgcgt
     2041 gcatcgctga cgagcctgct tggacgagtc ggcattgcca atgccaggct cgcgtatcaa
     2101 cactacctcg cgatg
//
LOCUS       DASURG010000449         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_284026, whole genome shotgun sequence.
ACCESSION   DASURG010000449 DASURG010000000
VERSION     DASURG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284026"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFY80_07885"
     CDS             <1..673
                     /locus_tag="VFY80_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX6007868.1"
                     /translation="RRAMDIEGLGEKLVDQLVEAGRVKTPADLYKLDVDVLATLDRMA
                     EKSAANVVAAIERSKQTTLARFIYALGIRNVGETTARDLARHFGGIEALMAADSESLQ
                     RVSDVGPVVAESIAQFFAEPHNRKVVRQLIEAGVTVENLPAAGRSRSLEGKSFVLTGT
                     LPSLTRDQAKERIEQHGGRVGGSVSKKTDYVVAGAEPGSKYDKALALGIPIIDEAQLL
                     ELLRD"
     gene            714..1595
                     /gene="galU"
                     /locus_tag="VFY80_07890"
     CDS             714..1595
                     /gene="galU"
                     /locus_tag="VFY80_07890"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX6007869.1"
                     /translation="MAKVNKAVFPVAGMGTRFLPATKASPKEMMPVVDKPLIQYAVEE
                     AVAAGITEMIFVTGRNKRPIEDHFDKAFELESELEQRGNKHLLQMVRNIIPRRVSCVY
                     LRQPQALGLGHAILCAASVVGNAPFAVVLADDLIDADPPVLRQMTDAFARRGASLIAV
                     QKVARTETRQYGIVKLDSRGGGDYRITGIVEKPAPSSAPSTLGVVGRYVLNPRIFEHL
                     RRVKPGAGAEIQLTDGIAALLEEEDVYAYEFSGVRYDCGSKLGYLKANVAYALKHPEV
                     GPDLRRYMRSLVSRLDH"
     gene            1648..2208
                     /locus_tag="VFY80_07895"
     CDS             1648..2208
                     /locus_tag="VFY80_07895"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF006605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEX6007870.1"
                     /translation="MKTDAAQAWSLLESAELLCPAGKVDATVERLASQITSRLRDCQP
                     VVLGVMRGSVIFAGRLLPLLRFPLEFDYLEVTRYAGSTRGGSIQWKVSPGTTVKDRVV
                     LVLDDILDQGYTLATIRERLLEAGARELYTAVFADKNIGTEKPVKPDFVGIEVPNRYV
                     FGFGMDVYGMWRNLPAVYALKDDGDG"
     gene            2220..2966
                     /locus_tag="VFY80_07900"
     CDS             2220..2966
                     /locus_tag="VFY80_07900"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX6007871.1"
                     /translation="MLGIIGGSGLTRLDALDHTRREVVTTPYGPPSSALVFGTVRRSE
                     VVFLARHGEQHTIPPHAVNYRANMWALHARKVLDVIAVAAVGGIRRDLAPGTLVIPDQ
                     IIDYTYARESTYFEGGEAGVTHIDFTRPYCEAMRERLLAAAVEAGEAVADGGTYGATQ
                     GPRLETAAEIDRMERDGADVVGMTAMPEAALARELGICYAAIAVVVNPAAGRGSGAAV
                     ISLEDMMAVLRPAIERVERIIECLAGRYGG"
     gene            2956..3489
                     /gene="def"
                     /locus_tag="VFY80_07905"
     CDS             2956..3489
                     /gene="def"
                     /locus_tag="VFY80_07905"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6007872.1"
                     /translation="MAVRPVLRMGDPRLLDRARAVAEFDTPELHALIADMEDTMAALN
                     GAGLAAPQIGVGLQVVIFGVESNPRYPDVEPVPKTVLVNPELVLLDEEMEEGWEGCLS
                     VPGMRGLVPRYRRLRYRGFDARGAAIDRTVSGFHARVVQHECDHLAGVLYPMRIRDLR
                     NFGFTEELFPGQDIAAD"
     gene            complement(3494..>4407)
                     /locus_tag="VFY80_07910"
     CDS             complement(3494..>4407)
                     /locus_tag="VFY80_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6007873.1"
                     /translation="RDQRRLAALYLLTVADIRGTSPKVWNAWKGKLLEDLFHATHRQL
                     TGDAVSLESSLQARQDEALAKLRLYALPPHAHDALWSKLDMSYFLRHTPQEIAWHTRL
                     LYYRSDTVKPVVKARLSPAGEGLQVMVYVADQKMLFARICSFFEGLSYDIFEAKIYTT
                     RHGYALDSLQVHDATNRSPQYRDLISYIEYELTERLERKTALPPLTRPRLSRQLRHFP
                     ISPEVNIQPDDKGVYRVLSVIAGDRPGLLSRIARVLNAYDINLHTAMINTLGSRAEDT
                     FFVTGPALSDPKTVVRFESDLLEELRT"
BASE COUNT          804 a         1305 c         1495 g          803 t
ORIGIN      
        1 acgccgtgcc atggacatcg aagggcttgg ggaaaaactc gtggatcaac ttgtggaagc
       61 cggcagggtg aaaacgcccg ccgatctgta caagctcgat gtggacgtac ttgcgacact
      121 cgaccgcatg gccgagaagt ccgcggccaa cgtggtggcc gcgatcgagc gcagcaagca
      181 aacgacactc gcgcgtttta tctatgcgct tggcatacgc aacgtgggtg aaacgacggc
      241 acgcgatctt gcccggcact tcggcggcat cgaggcgttg atggcagcgg acagcgaaag
      301 cctgcaacgg gtgtccgatg tcggcccggt ggtggcggaa agcatcgcac agttcttcgc
      361 ggagccgcac aatcgcaagg tcgttcgcca gctcatcgag gcgggcgtga cagtcgagaa
      421 cctgcccgct gccgggcgat cgcgcagcct ggaaggcaag agtttcgtgc tgacgggcac
      481 tttgccgtcg ctcacgcgcg atcaggcgaa agagcgcatc gagcagcacg gcggccgggt
      541 gggcggcagc gtgtccaaga agacggacta cgtcgtcgcg ggtgcggagc ccggcagcaa
      601 gtatgacaag gcgctggcgc tcggcatccc gattatcgac gaggcgcagt tgctggaact
      661 attacgcgat tagcataccg aagcagtagc acaataatag aggcgaagcc gacatggcga
      721 aagtgaacaa ggcggtgttt ccagtggccg gtatgggtac ccgatttttg cccgcgacca
      781 aggcgagccc gaaggaaatg atgccggtgg tggacaagcc gctcattcag tatgcggtgg
      841 aagaggccgt cgcagccggg atcacggaga tgatttttgt caccggccgg aacaagcgac
      901 cgatcgaaga ccacttcgac aaggccttcg agctcgagtc ggaactcgag cagcgcggca
      961 acaagcatct gctgcagatg gttcgcaaca tcatcccgcg ccgcgtatcg tgcgtctacc
     1021 tgcgccagcc gcaggcgctc ggtctggggc atgcgatcct ctgtgccgcc tcggtcgtcg
     1081 gcaatgcacc tttcgcggtg gtgctcgcgg acgatctcat cgacgcagac ccgcccgtgc
     1141 tgcggcagat gaccgatgcc ttcgcgcgaa gaggcgcttc gcttatcgcg gtgcagaaag
     1201 ttgcgcgaac ggaaacgcgc cagtatggca tcgtgaaact ggacagccgc ggcggtggcg
     1261 actaccgcat cacgggcatc gttgaaaagc cggcacccag ctcggcgcct tccacgctcg
     1321 gtgtggtggg acgctacgtt ctgaatcccc gcatcttcga gcatctgcga cgggtgaagc
     1381 cgggcgccgg cgcggagatc cagttaaccg acggcatcgc ggcgctgctc gaggaagagg
     1441 acgtctatgc ctacgaattc tccggcgtgc gctatgactg cggtagcaag ctcggttatc
     1501 tcaaggcgaa cgtcgcctac gcgctgaagc acccggaagt gggccccgat cttcgcaggt
     1561 acatgaggag ccttgtttcg cggctcgacc actagcgcgg gcgcattcga gagaacctct
     1621 tcgggagagt cctgctacac tgatcgggtg aaaaccgacg cggcccaggc gtggtctctg
     1681 ctcgaatccg ccgaactctt gtgtccggcc ggaaaagtcg atgcgaccgt ggagcggctg
     1741 gcgtcgcaga tcacgagccg gcttcgcgac tgccaaccgg tcgtactcgg cgtcatgcgc
     1801 ggcagcgtca ttttcgccgg acggcttctt ccgctgctgc ggtttccgct cgagttcgat
     1861 tatctggaag tgacgcgtta cgcgggctcc acgcggggtg gttccatcca gtggaaggtg
     1921 agtcccggca cgacggtgaa ggatcgtgtg gtcctggtgc tcgacgatat actcgatcag
     1981 ggttacacgc tcgccacgat tcgcgagagg ctgttggagg cgggtgcgcg ggaactctac
     2041 acggcggtgt ttgccgacaa gaacatcggc accgagaaac cggtcaagcc ggatttcgtc
     2101 ggcatcgagg tgcccaatcg ctatgtgttc ggcttcggca tggacgtata cggcatgtgg
     2161 cgcaaccttc ctgcggtcta cgcactgaag gacgacgggg acggctagac gcattcacca
     2221 tgctcggaat catcggcggc agtggactga ctcgactgga tgcgctcgac catacgcgcc
     2281 gcgaggtcgt caccacaccg tacggtccgc cctcatccgc gctcgtgttc ggaacagtac
     2341 gtcgcagtga agtggtgttc ctcgcgcgtc acggagagca gcacacgatc ccgccgcacg
     2401 ccgtgaacta ccgcgccaat atgtgggcgc tgcacgcccg gaaggtcttg gacgtgattg
     2461 cggtcgctgc agtgggtggg atacgacgcg accttgcgcc cggtacgctt gtcatcccgg
     2521 atcagatcat cgattacacc tacgctcgcg agagcacgta cttcgaaggc ggcgaagcgg
     2581 gcgtgactca catcgacttc acgcgcccct actgtgaggc gatgcgtgag cggctgctcg
     2641 ccgcggcggt cgaggcgggc gaggcggtcg cggacggagg cacgtatggc gcgacacagg
     2701 gtccgcgtct cgagacggct gcagaaatcg atcgcatgga gcgggatggc gcggacgtgg
     2761 tcggcatgac tgccatgccc gaggcggcgc tcgcgcgcga gctcggcatc tgttacgccg
     2821 ccatcgcggt cgtcgtcaat cccgctgcgg gccgaggaag cggcgccgca gtgatttcac
     2881 tggaggacat gatggcggtg ctgcggccgg cgattgagcg ggtcgaacgc atcatcgagt
     2941 gtctggcggg gcgctatggc ggttaggccg gtgctacgca tgggggaccc gcggcttctc
     3001 gatcgtgcgc gcgcggttgc ggagttcgat acccccgagc tgcacgcgct tatcgccgac
     3061 atggaggaca cgatggctgc gttgaacggg gccggcctcg ccgcacccca gatcggcgtt
     3121 ggtttgcagg tcgtcatctt cggcgtcgaa tcgaatccac ggtatccgga tgtggaaccg
     3181 gtaccgaaga cggtgctggt caatccggag ctggtgttgc tcgacgagga gatggaagaa
     3241 ggctgggaag gatgcctttc cgtaccgggc atgcgagggc tcgtgccgcg ctacaggcgg
     3301 ctgcggtatc ggggcttcga cgcccgcggc gccgcgattg accgcaccgt cagtggattc
     3361 cacgcgcggg tcgtccagca cgaatgcgac cacctggccg gcgtgcttta cccgatgcgg
     3421 atccgcgatc tccgcaattt cggctttacc gaggaactgt tccccgggca ggatatcgcg
     3481 gcggactagc ggcctacgtg cgcagttcct cgagcagatc gctctcgaag cgcaccactg
     3541 tcttggggtc gctcagcgcg gggccggtta cgaaaaatgt gtcctccgcg cgcgatccca
     3601 gcgtattgat catcgcggtg tgcaagttga tgtcgtaggc gttcagcacg cgcgcgatgc
     3661 gtgaaagcag tcccgggcga tctccggcga tgacggacag tacccggtag acgcccttgt
     3721 cgtcgggctg gatgttcact tcaggcgaga tcggaaagtg ccgcaattgc cggctcagac
     3781 gcggccgggt gagcggtggc agcgccgtct tccgctcaag gcgttcggtg agctcgtatt
     3841 caatgtagct gatgagatcg cgatactgcg gcgagcgatt cgtcgcgtcg tgcacctgta
     3901 agctgtcgag cgcataaccg tggcgcgtgg tgtagatctt ggcttcgaag atgtcgtagc
     3961 tcaagccctc gaagaagctg cagatgcgcg cgaacagcat cttctggtcg gccacgtaga
     4021 ccatgacctg caggccttcg ccggccggcg aaaggcgcgc cttcaccacg ggcttcacgg
     4081 tgtcgctgcg gtagtagagc aagcgcgtgt gccacgcgat ctcctgcggc gtatgccgca
     4141 ggaagtagga catgtcgagc ttcgaccaaa gcgcgtcgtg cgcatgggga ggcagcgcgt
     4201 agagccgcag cttcgcgagc gcttcatcct gccgtgcctg caggctgctc tcgagactca
     4261 cggcatcgcc ggtcagctgt cggtgggtcg cgtggaagag gtcttcgagg agttttcctt
     4321 tccaggcatt ccatactttg gggctggtgc cccgaatgtc cgccacggtg agcagataga
     4381 gcgccgcgag ccgtcgctgg tcccgca
//
LOCUS       DASURG010000450         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_78589, whole genome shotgun sequence.
ACCESSION   DASURG010000450 DASURG010000000
VERSION     DASURG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78589"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFY80_07915"
     CDS             complement(<1..650)
                     /locus_tag="VFY80_07915"
                     /inference="COORDINATES: protein motif:HMM:NF020475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HEX6007874.1"
                     /translation="MKYEDPACAYCPPTVRACRQGEEAARGPGFCPTKVDPETQAAAR
                     AQYDDPEIRRISRESALVESEGYCRWTRVEEAVQFAKRMGYRRIGIANCISFVDHAYV
                     LSGILESHGFEVVSASCKTGGIPKEEMGIADHEKVRPGQFEALCNPVCQAELLNAHGC
                     EFNIVLGLCIGHDSLFFKHAKGLTTVLIAKDRVLGHNPVAALHLADTYYSRVWGPD"
     gene            complement(744..1481)
                     /locus_tag="VFY80_07920"
     CDS             complement(744..1481)
                     /locus_tag="VFY80_07920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6007875.1"
                     /translation="MCDSPAKSGYRGWRGWLPLPATHIESPSGDWVDLTYPLSSEVPR
                     VASFAPPVFTRIAEMPEKPLNVTRMDTIVHMGTHLDAPRHFFLDGPAMEAVPLERCMG
                     RGVVLRIEKPAYGVIDVADLERAQPQIEPGDIVAIDTGWSATWGSPAWDRQPCVSVEA
                     ARWLRERRIKMLAVDTPTPDLPLDRREPDFTWPVHRTLLAHGILIAEQLANLESLSGR
                     RVEFMFGPLPIAGSDGSPARAIARAVS"
     gene            complement(1485..2723)
                     /locus_tag="VFY80_07925"
     CDS             complement(1485..2723)
                     /locus_tag="VFY80_07925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6007876.1"
                     /translation="MADATLGAYNIADLREMARRRLPKGMFEFVDRGTEDEIALANNR
                     AAFERIRFRPRTLVDVSTRSQEVTLFGRTMPMPIAVGPTGTAGLLWHQGEVGLARAAA
                     AAGIPFTVAINSMTSMEAIAEQAGGRLWFQLYMWSDRALSHQIVARAKAAGFEALIVT
                     VDGAVRANREYGHRNGFEMPFRFNRRNVAGVLARPGWLFHVLLRYALTSGIPRFENYP
                     PDIMDRLTARTLKSSFLKNDSLTWDDIRTLRRLWPGMLMVKGILHPQDAMLAADCGAD
                     GVIVSNHGGRMLDSAIAPIEVLPEIVDAVGQRITVLMDSGVRRGADVVKALALGARAV
                     LIGRATLYGVAAAGERGAARAIAILRDEVSRVMALIGAREIGELDPGFVELPPMPAQG
                     TARAALDTALRAVEPRVRAV"
     gene            2975..3445
                     /locus_tag="VFY80_07930"
     CDS             2975..3445
                     /locus_tag="VFY80_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX6007877.1"
                     /translation="MKAKWEGFTLVELMSVVAIIGILAAIALPLHMDYTKRSRMTEVV
                     LAVSACRTPVSDMYQTQKSAPGAGNWGCEVSAPASRYVQSITTDANGKIIATAQNFSD
                     PDIDGKVVTLVPLINGAAAVSASNMGKPITEWRCGSSADGTTVPVKYLPSSCRG"
BASE COUNT          753 a         1291 c         1231 g          657 t
ORIGIN      
        1 ttatccgggc cccatacgcg gctgtagtag gtatcggcga ggtgcagtgc ggcaacaggg
       61 ttgtgtccca gcacgcgatc cttcgcgatg agcacggtag tgaggccttt ggcgtgcttg
      121 aagaacagac tgtcgtgtcc gatgcacagc ccgagcacga tgttgaattc gcagccgtgc
      181 gcattgagca attcagcctg acacacggga ttgcagagtg cctcgaactg cccgggacgc
      241 actttctcgt gatccgcgat gcccatctcc tccttcggga tgccgcccgt cttgcacgag
      301 gccgaaacga cttcgaagcc gtggctttcc agtatgccgc tgaggacgta agcgtgatcc
      361 acgaagctga tgcagttcgc gataccgatc ctgcggtagc ccatgcgttt tgcaaactgg
      421 acggcctcct cgacgcgcgt ccatctgcag tagccctcgc tctctaccag tgcggattcg
      481 cgtgagattc tgcggatctc gggatcgtca tactgcgccc gggccgccgc ctgcgtttcg
      541 ggatcgactt tcgtgggaca gaaacccggt ccgcgcgccg cttcttctcc ttgccgacag
      601 gcgcggaccg taggcgggca ataggcgcag gcggggtctt cgtatttcat ggcggctccc
      661 gatagagcaa aacctgccgc cagtataggc aagcccctga gacgccattt gtcccagatc
      721 aaacggaacg gacgaggcag ccctcaggaa acggcgcgcg cgattgcccg cgcgggcgat
      781 ccgtccgaac ccgcaatagg cagcggtccg aacatgaatt ccacccgccg gccggagagg
      841 ctttcgaggt tcgcaagctg ctcagcgatg agaatgccgt gcgcgagcag cgtgcgatgc
      901 accggccagg tgaaatcggg ctcccgccga tcgagcggca aatcgggtgt cggcgtgtcg
      961 acggccagca tcttgatccg ccgctcgcgc agccagcgcg cggcctcgac cgaaacacac
     1021 ggctgccgat cccatgccgg cgagccccat gtggcgctcc agccggtatc gatcgccacg
     1081 atatcgcccg gctcgatttg cggctgcgcc cgctcgaggt ccgcgacgtc gatcacgccg
     1141 taggcgggct tctcgatgcg cagcacgacg ccgcgcccca tgcagcgctc gagcggcacc
     1201 gcttccatcg cggggccatc gagaaaaaaa tggcgcgggg cgtcgagatg cgtgcccatg
     1261 tgcacgatcg tgtccatgcg cgtcacgttg agcggtttct cgggcatttc cgcgatccgc
     1321 gtgaagacag gcggtgcaaa cgaggcaaca cggggtactt ccgatgacag cggatacgtg
     1381 agatcgaccc agtcgccgct cggcgattcg atatgcgtcg caggcagcgg caaccagcca
     1441 cgccagccgc gatacccgct cttcgcggga ctgtcgcaca tgcgctatac ggcccggacc
     1501 cgaggctcta cggcacgcaa cgccgtatcg agagctgcgc gcgcagttcc ctgcgcgggc
     1561 ataggcggaa gctccacgaa ccccgggtcg agctcgccaa tctcgcgggc gccgatgagc
     1621 gccatcacgc ggctcacttc gtcgcgcagt atcgcgattg cgcgagccgc cccgcgctcc
     1681 cccgccgcgg ctacgccgta gagcgtggca cgcccgatca gcaccgcgcg cgcccccagc
     1741 gcgagcgctt tcaccacatc cgcgccacgc ctgacaccgc tgtccatcag gaccgtgatg
     1801 cgctgaccga ccgcgtccac gatctcgggc agcacttcga tcggcgcgat ggcgctgtcg
     1861 agcatgcgcc cgccatgatt cgagacaatc acgccgtcgg cgccacaatc ggctgccagc
     1921 atggcgtcct gaggatggag gatgcccttg accatcagca tgccgggcca cagcctgcgc
     1981 agcgtgcgaa tgtcgtccca cgtgaggctg tcgttcttga ggaacgagct cttcaacgtg
     2041 cgcgccgtga gccggtccat gatgtccggg ggataattct cgaaccgcgg gatgccgctc
     2101 gtcagcgcat agcggagcag cacgtggaac agccagcccg gccgcgcgag caccccggcc
     2161 acgttgcggc ggttgaaccg gaacggcatc tcgaagccgt tgcggtggcc gtactcccgg
     2221 ttcgcccgca ctgcgccgtc cacggtcacg atcagcgctt cgaatcccgc tgccttggca
     2281 cgcgcaacga tctgatgcga gagcgcgcga tccgaccaca tgtagagctg gaaccagagg
     2341 cgcccgcccg cctgctcggc gatcgcttcc atcgaggtca tggagttgat cgctacggtg
     2401 aacggaatgc cggccgcggc cgcggcacgc gcaagtccca cttcgccctg atgccagagc
     2461 agccctgccg tgccggtggg gcccaccgca atcggcatcg gcatcgtgcg accgaaaagc
     2521 gtgacttcct gactgcgggt cgagacgtcc accagcgtgc gtggccgaaa ccggatgcgc
     2581 tcgaacgcag cgcggttgtt ggcgagcgca atttcgtcct cggtgccgcg atcgacgaac
     2641 tcgaacatgc ccttcggcag gcggcggcga gccatttcgc gcagatccgc gatgttatag
     2701 gctccaagcg ttgcgtcggc cacgtcaatt ctctccctgc ttcacggtac gcctatgtta
     2761 tacgcaacgg cctgcggcgc gcatgccgca gcgaaccgac ccgtggtaca ttcagactat
     2821 gcggccgcgc aagtttgagc cggcgccggg gcgcactaaa gaccattgag cgcccacgcc
     2881 gcacgacagg atgaagggac agggccgcca tgcgattgac gaattaacat gcgtgcccgc
     2941 tacagcggga gacaatgaca agaaaggcag tgcaatgaaa gcgaagtggg agggattcac
     3001 gctcgtcgag ctgatgagtg tcgtcgccat catcggcatc ctggcagcca tcgcgcttcc
     3061 cctgcatatg gattacacga agcgctcgcg catgacggaa gtcgtgctcg cggtaagtgc
     3121 ttgccggacc ccggtcagcg acatgtatca gacgcagaag agcgcaccgg gcgccggcaa
     3181 ctggggatgc gaagtgtcgg cgccggccag ccgctacgtc cagtcgatca ccaccgacgc
     3241 caacggcaag atcattgcga ccgcgcagaa cttcagcgat ccggacatcg acggaaaggt
     3301 cgttacgctg gtgccgctca tcaacggcgc agcggccgtt tctgcaagca acatgggtaa
     3361 gcccatcacc gagtggcgtt gcggcagttc cgcggacggt accacggtgc ccgtcaagta
     3421 cctgccgagc tcatgccgcg gctgaaccga ccgaaagggt aaacagccgc gagcgcacgc
     3481 aaagatcgcg aagcagagcc catgccccaa cttgcgtcaa tgctcaatct cagccggctc
     3541 gatgacattc tgcggacgct agtatgctag agggccgacg cgaacttacc gcagacgagg
     3601 tattccatga ccagccctgc acgacgtgaa gccgtccgtc gacatcaccg cttcaaggat
     3661 caactctcgg gtgatgtcga ataccggggt gcatcatacc cttgtgtcgt gaccgacatc
     3721 agccagcgag gcctgtatct cacatcgaac gtgccggtaa agctcggcga tcgaatgcgg
     3781 gtggacctta gaattgctcc agacagacgg ttctcatgca tgaccgaaat ccgacagatc
     3841 acgagcggcg ggctgggagc cgagatcgtc gacatcacgg acgaggacgc gcgtgtgctg
     3901 gtcggcagga tcgaagacca ttgttcggcc gt
//
LOCUS       DASURG010000451         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1099809, whole genome shotgun sequence.
ACCESSION   DASURG010000451 DASURG010000000
VERSION     DASURG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099809"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..700)
                     /locus_tag="VFY80_07935"
     CDS             complement(65..700)
                     /locus_tag="VFY80_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6007878.1"
                     /translation="MAYDLQEQEQLEAIKSWWEQYGTLIITVVVACLLTIAAFQGWRY
                     YRNQQSIAAVTLYEQLSQAERANEHKKVRDIALQITDKYSRTPYAAMAALSAGRAAFE
                     TGDLGAAKAQLQWVIDHAREEEMKDLARLRLAGVLLDEKNYDEALALLNAPHGAAFDA
                     LYADLKGDILLAQGKRGEARGAYQQALEKSDVKSPYRPMIQAKLDALGEAK"
     gene            complement(700..1956)
                     /gene="hisS"
                     /locus_tag="VFY80_07940"
     CDS             complement(700..1956)
                     /gene="hisS"
                     /locus_tag="VFY80_07940"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX6007879.1"
                     /translation="MNDILPDEAELWERFEGTVLKWVRAYGYRAIRTPILERTELFVR
                     SIGAVTDIVEKEMYTFIDELNGERLTLRPEGTASCVRAVIEHSLLYAGPQRLWYWGPM
                     FRHERPQKGRYRQFHQVGVEALGFPGPDIDAEHMLMCARLWRELGLEDVRLEINTLGS
                     SAARARYRTRLVAYLERHVDRLDEDSKRRLHANPLRVLDSKNPAMQAIIADAPKLADD
                     LDADSAASFEALKDRLRAADLQYVVNPRLVRGLDYYNGAVYEWLTDRLGAQGTVCAGG
                     RYDGLVEQIGGKPAPACGFAMGVERLLALMKNAGAMPSRAFPDVYLVHQGEAAGRLAE
                     AVAERLRDHELSVVLHCGGGGFKSQMKKADASGARFAVIVGDEEAQANEVSVKPLRTA
                     AAQTRVPLDQAIALIRRHELHTDREA"
     gene            complement(2007..3293)
                     /gene="ispG"
                     /locus_tag="VFY80_07945"
                     /gene_synonym="gcpE"
     CDS             complement(2007..3293)
                     /gene="ispG"
                     /locus_tag="VFY80_07945"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX6007880.1"
                     /translation="MSCYPSYGPQARRCSRKVRVGHVAIGGDAPIVVQSMTNTDTADL
                     QATVDQVAALARAGSEIVRVTVNNAEAAAQVPRIRDRLDRMGITVPLVGDFHFNGHRL
                     LTQHPACAEALAKLRINPGNVGRGSKRDEQFATMIEFALRYAKPVRIGVNWGSLDPEL
                     LARMMDENARRPEPEEAAVVTRKALVTSALESAREAQALGLPGDAIVLSCKVSGVQDL
                     IAVYRDLAARCDYALHLGLTEAGMGTKGIVASTAAMSVLLQEGIGDTIRVSLTPEPGG
                     DRTREVIVAQEILQTMGLRSFAPMVIACPGCGRTTSTFFQELADRIQNYLRQQMPLWR
                     ERYPGVESMHVAVMGCVVNGPGESKHANVGISLPGSGENPVAPVYVDGVKTVTLKGGN
                     IACEFQNIVEQYVVDHYSKPQAASTQSGEAPAKVLG"
     gene            complement(3290..>3841)
                     /locus_tag="VFY80_07950"
     CDS             complement(3290..>3841)
                     /locus_tag="VFY80_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEX6007881.1"
                     /translation="TPFSTHTEHRWPRVLMLTALLAVVALGAYEFYWYDRSTEDSPVS
                     AVAPIAIREPAAPSAPAPVSSAPAAASSAPPGPPAEPAVAPVEVTTPSANDGTPTPAI
                     SEEGALRFVFSRDSWVQVRDGTGKVIMTRLNAAGTEQTLSGKPPFSLVIGNARGVRLS
                     YNGESVDLGPHTVRDDVARLRLE"
BASE COUNT          630 a         1312 c         1201 g          698 t
ORIGIN      
        1 accgtctggc agcctacgag caggagcgca gccgctccgg cggcagccct acgcagcgcg
       61 cgcttcattt cgcctcgccg agcgcatcga gcttcgcctg gatcatcgga cggtaggggc
      121 ttttcacgtc gctcttttcg agcgcctgtt gataagcgcc gcgcgcctcc ccacgcttgc
      181 cttgcgcaag caggatatct cccttcaggt ccgcgtagag cgcatcgaac gcggcgccgt
      241 gcggcgcgtt caggagcgcg agcgcttcat cgtagttctt ctcgtcgagc agcacgccag
      301 cgagccgcaa gcgcgcgagg tccttcatct cctcttcgcg cgcgtggtcg atcacccact
      361 ggagctgcgc cttcgcggcg ccaagatcgc ccgtctcgaa tgcagcccgg cccgcgctca
      421 gcgcagccat cgccgcatag ggcgtgcgcg aatacttgtc ggtgatctgg agcgcgatgt
      481 cgcgcacctt cttgtgctcg ttcgcacgtt cggcctgcga cagctgctcg tacaaagtca
      541 cggcggcgat cgactgctgg ttgcggtagt aacgccagcc ctggaaggcc gcgatcgtga
      601 gcagacaagc gacaaccacg gtgatgatga gcgttccata ctgctcccac catgacttga
      661 tcgcctcgag ctgttcctgt tcctgtaaat cgtacgccat cacgcttccc tgtctgtatg
      721 tagttcgtgt cgccggatca acgcaatcgc ctgatcgagc ggcacgcgcg tctgcgccgc
      781 cgcagtgcgc agaggtttca cgcttacttc gttcgcctgt gcttcttcgt cgcccactat
      841 gaccgcgaaa cgggcgccgc tcgcatccgc tttcttcatc tgcgacttga agccgccgcc
      901 gccgcagtgg agcacgacgg agagctcgtg gtcgcgcagc cgctcggcga ccgcttccgc
      961 caggcggccc gctgcctcgc cctggtgcac gaggtagacg tcgggaaagg cgcgcgaggg
     1021 catcgcgccc gcgtttttca tcagcgcgag cagccgctcc acgcccatcg cgaagccaca
     1081 cgccggagcg ggtttcccgc caatctgttc gacaagaccg tcgtagcgac cccccgcgca
     1141 caccgtgccc tgcgcaccca gccggtccgt cagccactcg tagaccgcgc cgttgtagta
     1201 atcaagtcca cggacgagcc gcggattcac cacatactga agatccgccg cgcgcaggcg
     1261 gtccttgagc gcttcgaaac tcgcagcgga atcggcatcg agatcgtccg cgagcttcgg
     1321 tgcgtcggcg atgatcgcct gcatcgcggg attcttggag tcgagcacgc gcaacggatt
     1381 cgcatgcagg cgccgcttcg agtcctcgtc cagccgatcg acgtgccgtt cgagataggc
     1441 gacgagccgc gtccggtacc gggcccgcgc cgcgctgctt ccgagcgtgt tgatttcgag
     1501 ccgcacatct tcgagcccga gttctcgcca cagccgcgcg cacatcagca tgtgctcggc
     1561 gtcgatgtcg ggaccgggaa atccgagcgc ttccacgccc acctgatgga actggcgata
     1621 gcgtcctttc tgcggtcgct cgtgccggaa catgggcccc cagtaccaca gccgctgcgg
     1681 acccgcgtag agcaggctat gctcgatcac ggcacggaca caggatgcgg tgccctctgg
     1741 gcgcagtgtc aggcgctcgc cgttcagctc gtcgatgaag gtgtacattt ccttctcgac
     1801 gatgtcggtc acggcgccga tcgaacgcac gaaaagctcc gtgcgctcca ggatcggcgt
     1861 gcggatcgcg cggtagccgt aggcgcgtac ccactttaga acggtgccct cgaagcgctc
     1921 ccacagctcc gcctcgtccg gcaggatatc gttcatcccc cggagcgcac ttattttttg
     1981 tgtcatgtgc gattgcggcg cagcgtctag ccgagcactt tcgccggcgc ctcgccggac
     2041 tgcgtcgatg ccgcttgggg tttggagtag tgatcgacga catactgctc gacgatgttc
     2101 tggaattcgc acgcgatgtt gccgcccttg agcgtaaccg tcttgacgcc atcgacgtag
     2161 accggtgcga cgggattttc tcccgagccc ggcaggctga tgccgacgtt cgcgtgcttg
     2221 ctctcccccg gtccgttcac gacgcagccc atgactgcga cgtgcatcga ttcgacgccg
     2281 ggatagcgct cacgccagag cggcatctgt tggcgcaggt agttttggat gcggtccgcg
     2341 agctcctgga agaacgtgct ggtcgtccgt ccgcaacccg ggcaagcaat caccatgggc
     2401 gcgaacgatc gcagccccat cgtctgcaat atctcctgcg caacgatgac ttcccgcgtg
     2461 cgatccccgc cgggctccgg cgtcagcgat acgcgtatcg tgtcgccgat gccttcctgc
     2521 agcaatacgc tcatcgcggc ggtcgaggca acgatgcctt tggtgcccat gcccgcctcg
     2581 gtgagcccga gatggagcgc ataatcgcag cgcgccgcga gatcacggta gacggcgatc
     2641 aaatcctgca cgccgctcac cttgcatgag agcacgatgg catcgccggg aaggccgagc
     2701 gcctgcgcct cgcgggcgct ctccaacgcc gaggtgacca gcgccttgcg cgtcaccact
     2761 gcggcctcct ccggttcagg gcggcgcgcg ttctcgtcca tcatccgcgc aagaagctcc
     2821 ggatcgaggc tgccccagtt cacgccgatc ctcaccggct tcgcatagcg caacgcaaac
     2881 tcgatcattg tcgcgaactg ctcgtcgcgc ttcgagccgc ggccaacgtt gccgggattg
     2941 atgcgcaact tggcgagggc ttccgcgcac gccggatgct gcgtgaggag ccggtgaccg
     3001 ttgaagtgaa agtccccgac cagcggaaca gtgatcccca tgcgatcgag acgatcccgg
     3061 atgcggggga cctgcgctgc ggcttcggcg ttgtttacag tgactcgcac gatctcggag
     3121 cccgctcgcg caagcgctgc cacctgatcc accgtcgctt ggaggtccgc cgtatcggtg
     3181 ttggtcatcg actgcacgac gatcggcgcg tcaccgccga tcgcgacgtg accgacgcgc
     3241 accttgcggc tgcagcgccg cgcctgggga ccgtaggagg gatagcagct cattcgagcc
     3301 tcagccgcgc aacgtcgtcg cgcacggtgt gcggaccgag atccaccgac tcgccgttgt
     3361 agctcaaccg cacaccgcgc gcgttgccaa tcacgagcga aaaaggcggt ttcccggaaa
     3421 gggtctgctc ggtacccgcc gcgttgaggc gggtcatgat gacctttccg gtgccatcgc
     3481 gcacctgcac ccacgagtcg cggctaaaga cgaagcgcag cgcgccttcc tcggatatcg
     3541 caggcgtcgg cgtgccatcg ttcgcgctcg gcgtggtcac ctcgacgggc gctacggcag
     3601 gctctgccgg ggggcccggt ggcgcgcttg atgccgcggc aggagcggac gagacggggg
     3661 cgggcgcgga aggcgccgcc ggttcccgaa tggcaattgg cgcgaccgcg cttaccggag
     3721 agtcttccgt gctgcgatcg taccagtaga actcgtaggc tcccaacgca acgacagcga
     3781 gcagcgccgt caacatcagc acacgcggcc agcggtgttc cgtatgcgtg gaaaacggcg
     3841 t
//
LOCUS       DASURG010000452         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_78983, whole genome shotgun sequence.
ACCESSION   DASURG010000452 DASURG010000000
VERSION     DASURG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78983"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFY80_07955"
     CDS             <1..888
                     /locus_tag="VFY80_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6007882.1"
                     /translation="MHNGSPVFIIVHMNPTHRSAREEIKQALGERFGAEFDVPPGLPG
                     AEELARIAGHRSHRKYRAEPVLPELLRLLCACALSAPSKSDLQQADIVHVADRARLKA
                     IVDLIPDMPWINAAPVFLVFCGNNRRIRRIGEWRGKPFANDHLDHFMNAAVDAGLVMM
                     NFIRAAEAVGLGACPISAVRNRPHEVSALLDLPDCVFPVAGLTVGYPAEAGRISARLP
                     LDVTLHVDRYDERDLKRRIEHYDRWRRELQPFRKQRHSDRYEDVEDYGWSEDKARQYA
                     VPERPDFGAFIRAKGFTLD"
     gene            complement(956..2308)
                     /gene="ffh"
                     /locus_tag="VFY80_07960"
     CDS             complement(956..2308)
                     /gene="ffh"
                     /locus_tag="VFY80_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX6007883.1"
                     /translation="MFENLTQRLTRVMKTLRGEARISEANIQDALREVRVALLEADVA
                     LPVVRDFIARVREKALGREVLGSLTPGQAVVGVVHKELVHLMGERADALALATKPPAV
                     ILMAGLQGSGKTTTSAKLAKWLGDGERKKVLLVSCDVYRPAAIDQLQTLAAQIGVDFH
                     PASAGEKPVAIAKAALDFARTHYHEVLIVDTAGRLAIDEAMMSEIRELHAALNPAETL
                     FIVDAMQGQDAVNTAKAFSEALPLTGILLTKLDGDARGGAALSVRHVTGKPIKFAGVG
                     EKLTGLEPFHPDRMASRILGMGDVLTLIEDVQKGVDREQAEKLAKKVKSGKGFDLEDF
                     KQQIVQMKKMGGLAALADKLPGNLAQMAQGAPQMDDKGIRRIEGIINAMTPGERSKPD
                     IIKASRKRRIASGAGVTVQEVNRLLNQFEQTQKMMKMMAKGGLTKMMRSMKGMFPGMR
                     "
     gene            2337..>2914
                     /locus_tag="VFY80_07965"
     CDS             2337..>2914
                     /locus_tag="VFY80_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEX6007884.1"
                     /translation="MPAILPYLVNALLYGGLGYHFWRTRWAHAGPAQAHSVPSAVEHY
                     AMLVPLGLHTALLARSMFGGQGLYLGVGDAVSAILWLTVLIYWIGNFHYRLEGLQALV
                     MPVAAVASLLPAVLPSLKPLPNTEFLAFKFHLLISMLAYSLFTIASLHVLLMALLERR
                     LHDGTLPQVLQRLPPLLAMESVLFRVIWAGFVL"
BASE COUNT          466 a          979 c          904 g          565 t
ORIGIN      
        1 atgcacaatg gcagccccgt attcatcatc gtccacatga atcctaccca tcgcagcgct
       61 cgtgaagaga tcaagcaggc gctcggcgag cgtttcggcg ccgaattcga tgtaccgccg
      121 ggcctgccag gcgccgagga actcgcccgg atcgccggcc acagatcgca ccgcaagtac
      181 cgggccgaac cggttttgcc ggagcttctg cggctactct gcgcgtgcgc gctctcggcg
      241 ccttcgaaaa gcgatctgca acaggccgac atcgtgcacg tcgccgaccg tgcgcgcctg
      301 aaggcgatcg tcgatctcat tcccgacatg ccatggatca acgcggcgcc ggtgttcctc
      361 gtgttctgcg gcaacaaccg ccgcattcgg agaatcggcg agtggcgcgg gaagcccttc
      421 gccaacgatc acctcgacca tttcatgaat gcggcggtgg acgcgggact cgtcatgatg
      481 aatttcattc gcgccgccga agcagtcgga ctcggcgcct gcccgatcag cgccgtgcgc
      541 aaccgcccgc acgaggtgag cgcgctcctg gacctgcccg actgcgtgtt tccggtcgcc
      601 gggctcacgg tcgggtatcc ggcggaagcg gggcgcatca gcgcgcgcct gccgctcgac
      661 gtaacgctgc acgtcgatcg ttacgacgag cgcgatctca agcgccggat agagcactac
      721 gaccggtggc ggcgcgaact tcagccgttt cgcaaacaac ggcacagcga ccgctacgag
      781 gacgtggaag actacggctg gtcggaggac aaggcgcggc agtacgcggt gccggagcgc
      841 ccggactttg gcgcattcat ccgcgcgaag ggcttcactc tcgactgata gagcaggcgg
      901 gctacgtcaa cgagagaaca gtgcccgcag ccggttgtcc cggatgcagg gttcactacc
      961 gcatgccggg aaacatgcct ttcatcgagc gcatcatctt cgtaagcccg cccttcgcca
     1021 tcatcttcat catcttctgc gtctgctcga actggttgag cagccgattc acttcctgca
     1081 ccgtaacccc ggcgccgctc gcgatgcggc gcttgcgcga agctttgatg atgtcgggtt
     1141 tgctgcgctc gcccggggtc atggcgttga tgatgccttc gatacgccgg atgcctttgt
     1201 catccatctg cggtgcgccc tgcgccatct gtgcgagatt gccggggagc ttgtcggcga
     1261 gcgcggcgag cccgcccatc ttcttcatct gcacgatctg ctgcttgaag tcctcgagat
     1321 cgaagccttt gccggacttc accttcttcg cgagcttctc cgcctgctcg cgatcaacgc
     1381 ccttctgtac gtcctcgatg agcgtaagca cgtcgcccat gccgaggatg cgcgaggcca
     1441 tgcgatcggg atgaaacggc tcgagcccgg tcaacttctc tcccacgccg gcgaatttga
     1501 tcggcttccc ggtcacatgg cgcaccgaga gggcggcgcc gccgcgcgca tcgccgtcga
     1561 gcttggtgag gaggattccg gtcagcggga gcgcctcgga gaaggccttc gccgtgttga
     1621 ccgcatcctg gccctgcatc gcatcgacga tgaagagcgt ctcggccggg ttgagcgccg
     1681 cgtgcagttc gcggatctcg ctcatcatcg cctcgtcaat cgcgagacga cccgcggtat
     1741 cgacgatgag cacctcgtgg tagtgcgtgc gcgcgaaatc aagtgccgcc ttcgcgatgg
     1801 cgaccggttt ctcgccggcc gacgcgggat ggaaatcgac gccgatctga gccgcaagcg
     1861 tctgcaactg gtcgatggcg gcgggacgat agacgtcgca gctcacgagc agcactttct
     1921 tgcgctcacc gtcgcccagc cacttcgcga gcttggcgct ggtcgtggtc ttgcccgatc
     1981 cctgcagccc ggccatgagg atcaccgcgg gcggcttcgt cgcaagcgca agcgcgtcag
     2041 cgcgctcgcc catcaagtgc acgagctcct tgtgcaccac gccgacgacg gcctggccgg
     2101 gcgtgagact gccgagcact tcccggccca atgccttttc gcgcacgcgt gcgatgaagt
     2161 cgcgcacgac ggggagcgcg acgtccgctt ccagaagtgc gacgcgcacc tcgcgcaacg
     2221 catcctggat gttcgcttcg ctgatgcgcg cctcgccgcg cagcgtcttc atgacgcgcg
     2281 tcaggcgctg ggtgagattc tcgaacacgg gatgacgata aggtaaactc tgcggaatgc
     2341 ccgcgatttt accctatctc gtcaacgcct tgctctatgg cggcctcggg tatcacttct
     2401 ggcgcacgcg ctgggcgcac gccggtccgg cacaggcgca cagcgtgcca agcgcggtcg
     2461 agcactacgc aatgctcgtg ccactcgggc tgcacacggc gcttctcgcg cgttcgatgt
     2521 tcggcggcca gggcctctac ctcggcgtgg gcgatgccgt gtcggcgatt ctctggctga
     2581 cggtgctcat ctactggatt ggaaattttc attaccgcct cgaaggactg caggcgctcg
     2641 tgatgcccgt tgccgccgtg gcctctttgc tgcccgcggt tttgccttcg ttgaaaccgt
     2701 tgcccaacac ggagtttctc gcgttcaagt ttcacctgct gatctcgatg ctcgcctaca
     2761 gcctcttcac catcgcctcg ctgcacgtgc tgctgatggc gctgctcgag cggcggctgc
     2821 acgatggcac gctgccccag gtgctccagc ggctgccccc gctcctcgcc atggagtccg
     2881 tgctcttccg cgtgatctgg gcggggttcg tcct
//
LOCUS       DASURG010000453         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1510385, whole genome shotgun sequence.
ACCESSION   DASURG010000453 DASURG010000000
VERSION     DASURG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510385"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFY80_07970"
     CDS             complement(<1..744)
                     /locus_tag="VFY80_07970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6007885.1"
                     /translation="MLLSHCKSTRARRGWYGSLAPRNGMMAMRIGFIGLGQMGRCMAA
                     RLLERGYDLSVWNRRPAACDALRQLGAQVASRPQALLEADVVITMLADDAAVEAVWIA
                     SGMVRAAPSSSIHLNMASVSLGMGRRLAALHRESGSHYVSAPVFGRPPSAANGQLDII
                     AAGEEAALTRCAPLFVALGRRWFNVGTEPHRANVVKIARNFLLGSIIESLGESFALVA
                     KSGVDPATFLEILMSTSMNCPAYRNYGSLM"
     gene            813..1607
                     /locus_tag="VFY80_07975"
     CDS             813..1607
                     /locus_tag="VFY80_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6007886.1"
                     /translation="MDLGIKGKSAIVTGGSRGIGRETARQFLEEGVRVTICGRNSETL
                     ERTREDLLKRTGGEILAVTADMTKPDDIGRLVESARRQYGAVDILVNNAGQMYSGRFD
                     VLTDAGLKEQLETKLFGFMRAIRLVYPMMKAQRWGRIVNLIGGAGKEPDPYMFGSGIT
                     NSALLNVTKSLSAEFGEDNVLVNAVCPGWVDTNLWRRNAQGLAAELGAKSEEEARRLA
                     ARRNSLNRFGRPEELANAIVFLCSERASYITGVSLNLDGGRLKSLW"
     gene            1669..2637
                     /locus_tag="VFY80_07980"
     CDS             1669..2637
                     /locus_tag="VFY80_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007887.1"
                     /translation="MKLVRIAALLSAAILAALPLALRAQDYPTRSIRLIIPWPPGGIT
                     DVIARGLGATLSESLGQQVIPDNRPGAAGTLGVGIAAKSNPDGYTLLMTDVPSHAISA
                     TLYKSLPYDPIKDVEPVALPSRSPLVLVVNPKLGIKSIPQLIQHAKAKSDFSFASSGP
                     GSITHLTAERFIRETGIKPLHVPYKGGGPATAALVSGEAGMYFACISAAIPHIKSGRL
                     TLLGITSPQRSPLYPDTPTVAEVLPKFEMGCNTGFFAPGGTPKRIIDRLHADIMKAVK
                     QPKMKELLAANAAEPAPYTPAQFKQHVADEIREWGKVVRAAGLKVE"
     gene            2648..3403
                     /locus_tag="VFY80_07985"
     CDS             2648..3403
                     /locus_tag="VFY80_07985"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6007888.1"
                     /translation="MGELSGKVALVTGASRGIGRAVAERLAREGAAIYLAADGTEQEL
                     EEAARGCRAAGAPDAARAVHDLARDGEAEAMIAAAHQRMRRIDILVNNAGLRVRKAFG
                     EFTHEEFDRLVAVNLRAPFFASQAVVPIMRAQGGGCIVHMASQLGLVASRYGAVYNLT
                     KAGLIQLTRSMALELAVENIRINAVCPGPITTEGFLAGRNPGELEQRARDVPIGRFGT
                     AEEVAGVVAFLVSKDAGYVAGHALVMDGGYIVH"
     gene            complement(3527..>3916)
                     /locus_tag="VFY80_07990"
     CDS             complement(3527..>3916)
                     /locus_tag="VFY80_07990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6007889.1"
                     /translation="LRSVFLGSRAAVRRMLPRKRGVVVSTASGTGIRGQPKGSAYSAS
                     KAGIIGFTRAVSLELKDTGVRINCFGPGATDTPLWRVGKTEEEIKRLLAAGYVYQPDD
                     FSNVVVFLASDMSWPLNGEFISRDLHR"
BASE COUNT          752 a         1297 c         1239 g          628 t
ORIGIN      
        1 catcaggctg ccgtaattcc tgtaagccgg gcagttcatc gacgtgctca tgaggatttc
       61 gagaaacgtg gcgggatcga cgcccgactt cgcgacgagc gcgaaggatt caccgaggct
      121 ctcgatgatc gaccccagga gaaaattgcg cgcgatcttg accacgttgg cgcggtgggg
      181 ctccgttccg acgttgaacc agcgccgccc gagcgctacg aagagcggcg cacagcgggt
      241 gagcgctgcc tcttcgccgg ccgcgatgat atcgagctgt ccgttcgccg ccgacggcgg
      301 ccgcccgaac accggcgctg agacgtagtg tgaacctgac tcgcgatgga gcgcggcaag
      361 ccggcgcccc atgcccaggc tcaccgaggc catgttgaga tgtatcgacg aagacggcgc
      421 agcgcgcacc atccccgacg cgatccacac cgcttcaacg gcagcatcgt ccgcgagcat
      481 cgtgatgact acatccgctt cgagaagggc ctgcggtcgg cttgcgacct gcgcgccgag
      541 ctgccgaagc gcgtcgcacg cggccggcct tcgattccat acgcttaagt catagccgcg
      601 ctcgagcagc cgcgcggcca tgcagcgacc catttgaccc aacccgatga atccgatcct
      661 catggccatc atcccattgc gcggagccag cgagccatac cagccgcgcc gagcccgcgt
      721 tgatttacaa tgtgacagca acatgccgtc gcagccgcca ttatgacgaa aacaccgcgg
      781 ctcatttcac agtctctatc gcaaggaaag caatggatct gggaatcaag ggaaagagcg
      841 caatcgtcac cggcggcagc cgcggcatcg gtcgcgagac ggcacgccag ttcctcgaag
      901 aaggggtgcg cgtgacgatt tgcggccgca actccgaaac gctcgaacgc acgcgcgagg
      961 atctgctcaa gcggacgggc ggcgagatcc tcgccgtgac cgccgacatg acgaagcccg
     1021 acgatatcgg acggctcgtc gagagcgcgc gccgccagta cggcgcggtg gatatcctcg
     1081 tcaacaatgc gggacagatg tactccggcc ggttcgacgt cctgaccgac gccggactca
     1141 aggagcagct cgaaaccaag ctcttcgggt tcatgcgggc gatccgcctc gtgtatccga
     1201 tgatgaaggc gcagcgctgg gggcgcatcg tgaatttgat tggcggcgcg ggaaaggagc
     1261 cggatccata catgttcgga agcggcatca ccaacagcgc gctcctcaat gtcaccaaat
     1321 cgctctcggc ggaatttggc gaggacaatg tgctggtgaa cgcggtctgt cccggatggg
     1381 tcgataccaa tctttggcgg cgcaatgccc agggactcgc cgccgaactc ggcgccaaaa
     1441 gcgaagagga agcgcgacgg cttgccgcac gcaggaattc gctcaaccgc ttcggacgac
     1501 ccgaggagct cgcgaacgcg atcgtgtttc tctgttccga gcgcgcgagc tatatcaccg
     1561 gggtttcgct caacctcgac ggagggcgcc tgaagtcgct ctggtagccc cggtccgaca
     1621 cgatttggtc acgcttcgac acacgttcaa tcaacgagga ggaaagtgat gaagctcgtt
     1681 cgcattgccg ccttgctgtc cgccgcaatc cttgccgcgc tgccgctcgc gctcagagcg
     1741 caggactatc ccacgcgctc catccgtctc atcattccgt ggccgcccgg cggcatcacc
     1801 gacgtgatcg cgcgcgggct cggcgcgaca ttgagcgagt cgctcggcca gcaggtgatc
     1861 ccggacaacc gccccggtgc cgcaggcacc ctcggcgtgg gcatcgctgc gaagtcgaat
     1921 cccgacggct atacactgct catgaccgat gtgccgagcc acgcgatcag cgcgacgttg
     1981 tacaagagct tgccgtacga tccgatcaag gacgtcgagc ccgtcgcgct gccctcgcgc
     2041 tccccgctgg tgctggtcgt caatcccaag ctcggcatca agtcgatccc gcagctcatc
     2101 cagcacgcca aagcgaaaag cgacttctcg tttgcctcct ccggcccggg ctcgatcacg
     2161 cacctgacgg cggaacgctt catccgcgaa accggaatca agccgctgca cgtgccgtac
     2221 aagggcggtg gaccggcgac cgcggcgctc gtatcgggcg aggccggcat gtacttcgcc
     2281 tgcatttcag ccgcgattcc gcacatcaag tcgggacggc tgacgctgct gggcatcact
     2341 tcgccccagc gctccccact ttatccggat acgccgaccg tcgcggaagt cctgcccaag
     2401 ttcgaaatgg gctgcaacac cggcttcttc gctccaggcg gcacgccgaa gcgcatcatc
     2461 gatcggctgc acgccgatat catgaaagcg gtgaagcagc cgaagatgaa ggaactgctc
     2521 gcggcgaacg ccgcggaacc cgcaccgtac accccggctc agttcaagca gcacgtcgcc
     2581 gatgaaattc gcgaatgggg caaggtcgta cgcgcggccg gcctgaaggt cgagtaagac
     2641 cgccccgatg ggcgaactca gcggaaaagt cgcgctcgtg accggcgcct cacgcggcat
     2701 cggacgcgcg gtcgccgagc ggctcgcacg ggaaggtgcg gcgatctatc tcgccgcgga
     2761 cggcaccgag caggagctgg aagaggcggc ccggggatgc cgcgcggcgg gcgcacccga
     2821 cgctgcgcgg gccgtccacg atctcgcacg cgacggagaa gcggaggcga tgattgccgc
     2881 cgcacaccag cgcatgcggc gcatcgacat cctcgtcaac aatgcaggac tgcgggtgcg
     2941 caaggcgttc ggcgaattca cgcacgagga attcgaccgc ctcgtcgcag tgaacctgcg
     3001 cgcacccttc ttcgcgagcc aggcggtggt gccgatcatg cgggcgcagg gcggcgggtg
     3061 catcgtgcac atggcaagcc agctcggcct ggtcgcctcg cgttatggcg ccgtgtacaa
     3121 ccttaccaag gcgggactga tccagctcac gcgttcaatg gcactcgagc ttgcggtgga
     3181 gaacattcga atcaatgcgg tctgcccggg acccattacc accgaaggct ttctcgccgg
     3241 ccgcaatccc ggcgagctgg agcaacgggc gcgcgacgtg ccgatcgggc gcttcggcac
     3301 cgccgaggaa gtggcgggcg tagtggcgtt tttggtatct aaagacgccg gctacgtcgc
     3361 cggccatgcg ctcgtgatgg acggcggcta catcgtgcac tgaagcgtca cactgtccca
     3421 cggcgaaggc ctgtgtccgc gacgcttcct cttcccggcg caagccgaga tccaggcagg
     3481 aacgccggta atactggaat ccggctttcg ccggaatggc gacccgctag cggtgcaggt
     3541 cgcggctgat gaactcgccg ttgagcggcc acgacatgtc gctcgcaagg aacaccacga
     3601 cgttcgagaa atcgtccggc tgataaacgt atccagcggc gagcaggcgc tttatctctt
     3661 cttcggtctt tccgacgcgc cagagcggcg tgtcggtcgc acccggtccg aaacagttga
     3721 tgcgcacgcc ggtgtccttt aactcgagcg acaccgcgcg cgtgaacccg atgatccccg
     3781 cttttgaagc cgaatatgcc gaccccttgg gctgaccgcg gatgccggtg cccgaagcgg
     3841 tgctcacgac gacgccgcgc ttgcgcggca gcatgcggcg gaccgcggca cgcgagccca
     3901 agaacacgct gcgcag
//
LOCUS       DASURG010000454         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_794201, whole genome shotgun sequence.
ACCESSION   DASURG010000454 DASURG010000000
VERSION     DASURG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794201"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFY80_07995"
     CDS             <1..227
                     /locus_tag="VFY80_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6007890.1"
                     /translation="LEGQILMPYITGRRLTLNPVVIFLSMLLWGWLWGVIGMLLAVPI
                     AMILRIVCDHVEWLQPFGEFLSSERTEVPD"
     gene            complement(249..1292)
                     /locus_tag="VFY80_08000"
     CDS             complement(249..1292)
                     /locus_tag="VFY80_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6007891.1"
                     /translation="MARNSAGEFGGAALRLRAGWRGGRKAAGVIAVLAAAIAPVSAQG
                     LAEGDPTEQVVRVPVRATANGVPAEWSLVATLYRPRGAGPFPVVVLNHGSPGAAWERQ
                     AMGRYRPLPQVREFIKRGFVVIAPMRRGYGDTGGDWAERYGTCVAPDYYRAAREGAKD
                     VNATVSYARSLPFVRGDEVLLVGQSAGGIAAMAAASENPSGVVAVINFSGGRGGRPYT
                     HPGDPCVAANMTEAIGKLARTIEVPVLWHYSENDQFFAPRHVKAWYQAFRDAGGSGKL
                     VMQPPFQWDGHGIFGAERGLPIWTAAFDSFLREVRVAPITPPAVIPVAGQGGAAKPAR
                     PAAVPATPPGSDT"
     gene            complement(1572..2393)
                     /locus_tag="VFY80_08005"
     CDS             complement(1572..2393)
                     /locus_tag="VFY80_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6007892.1"
                     /translation="MERTKISRAIAWALLSSGVAVSGAAYAADSAKSTSSPTDRGVQS
                     RSAGTDRQATQQQGDRFWASDLIGKNVQLSGGKDGEIKDVIVDAKTGEVRHVVTEIDT
                     DRGKDGLYAVPARMFQLGKDNKLTLNVDQSWLAQRKPFTEDKWPAMQDKDYWGDTQAG
                     DNAHRLSKLIGQNVHNAEGKQVGEIEDAVVNLKSQKVDFVVFSHDPGVTKAEKDYALP
                     LASLQIPTATADGGEGKQRIVLNMSDEKLKGMKSLEDADRKRINDPAFMSRFQAQ"
     gene            complement(2601..2870)
                     /locus_tag="VFY80_08010"
     CDS             complement(2601..2870)
                     /locus_tag="VFY80_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX6007893.1"
                     /translation="MKREMLLGLLAAAVYCGSPLPAAAQVFKCKQPNGTVVYQDGPCE
                     GAAVVDTRSASGVSGGKSASSGGGTGSGAASTKPTHPVLPEKMRR"
     gene            3065..>3423
                     /locus_tag="VFY80_08015"
     CDS             3065..>3423
                     /locus_tag="VFY80_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HEX6007894.1"
                     /translation="MAEGSAKFHEDAELLTPETRDRHRAIASIMEELEAVDWYDQRVE
                     ATRDAELRAILAHNRDEEKEHAAMVLEWLRRRDSHLDEQLRNYLFIDKPVLEREEELD
                     QAEAGADAPSVPGAGEGS"
BASE COUNT          617 a         1118 c         1042 g          646 t
ORIGIN      
        1 ttctcgaagg gcagatcctg atgccctaca tcaccggacg gcggctcaca ctcaatccgg
       61 tggtgatttt tctgtcgatg ctgttatggg gatggctgtg gggcgtcatc ggcatgctgc
      121 tcgcggtgcc gatcgccatg atcctgcgca tcgtctgcga tcacgtcgaa tggctccagc
      181 cattcggcga attcttgagc agcgagcgga ccgaggtgcc ggactgaagc gctgcccgcg
      241 cgctgttctc aggtgtccga cccaggcgga gtagccggca ctgcagcggg gcgagcgggc
      301 tttgcggctc caccctgacc cgcaaccgga atgacggctg gcggcgtgat cggggcaact
      361 cgcacctcgc ggaggaagct gtcgaacgct gcggtccaga tcgggagtcc ccgctcggcg
      421 ccgaagatac cgtggccgtc ccactggaag ggaggctgca tcacgagctt gccgctgcca
      481 ccggcatcac gaaatgcctg gtaccacgcc ttcacgtggc gcggcgcaaa gaactgatcg
      541 ttttccgagt agtgccagag caccggcacc tcgatcgtgc gcgcgagctt gccgatcgcc
      601 tcggtcatgt tggccgcgac gcacgggtcg ccggggtggg tgtagggacg ccccccacgc
      661 ccgccagaga agttgatgac tgcgacgacg cccgacgggt tctcgctcgc ggctgccatc
      721 gccgcgatgc cgcccgccga ctgtccgacg agcagcacct cgtcgccccg cacgaaaggc
      781 agcgaacgcg catacgacac cgttgcattg acgtccttcg cgccctcgcg cgccgcacgg
      841 tagtaatccg gcgcgacaca cgtgccatag cgctcggccc aatcgccgcc ggtatcgcca
      901 tagccgcggc gcatgggcgc gatgactacg aatccgcgct tgatgaactc gcgaacctgc
      961 gggagcggcc gatagcgccc catggcctgg cgctcccaag cggctccggg actgccgtga
     1021 ttgagcacaa ctacgggaaa cggccctgcc ccgcgtggcc gatagagggt ggcaacgagg
     1081 gaccattctg cgggtactcc gttggcggtt gcacgcaccg gaacgcgcac cacctgctcg
     1141 gtgggatccc cttccgcgag gccctgtgcg gagaccgggg cgatcgccgc cgcgagtaca
     1201 gcgatcaccc ctgccgcctt ccgcccgccc ctccagcccg cgcgcagccg aagcgctgca
     1261 ccgccaaact ccccagcaga atttcgtgcc acctgcgagc ctctccctga tccgcgccgt
     1321 tcatgctcaa cgacaagctt gtcgtacgag cgctacagac tgtggcggaa tcttacaggc
     1381 gcgcaggcgg ggcgcaagcc ccctgtagaa atttttaggc gtgcacgcaa gcggcagccc
     1441 cggtgtgagt aaaccgcatg cggagaaacg atggacgaat tagaaatagt aatggcccgg
     1501 accgccgtcg ggcagcccga gccgttgaac gcgggaccct caccgagggc ccgatccgga
     1561 aagacgctcc tctactgagc ttggaaacgt gacatgaaag ccggatcgtt gatgcgtttc
     1621 cggtccgcgt cttccagcga tttcatgcct ttcaattttt cgtcactcat gttgagaacg
     1681 atgcgctgct tgccttcgcc gccgtcggcg gtcgcagtcg ggatctgcag cgaagcgagc
     1741 ggcagggcgt agtctttctc cgccttggtc acccccggat catggctgaa caccacgaag
     1801 tccactttct gcgatttcag gttgaccacc gcatcctcga tctcgccgac ctgcttgccc
     1861 tcggcgttgt gcacgttctg cccgatcagc ttgctcaggc ggtgtgcatt gtcgcccgcc
     1921 tgggtgtcac cccagtagtc cttgtcttgc atcgcgggcc acttgtcttc ggtgaagggc
     1981 ttgcgctgcg cgagccagct ttgatccacg ttcaacgtga gcttgttgtc tttcccgagc
     2041 tggaacatgc gcgccggaac ggcatacaac ccatcctttc ctcgatcggt atcgatctca
     2101 gtcacgacat gacgcacttc gcccgtcttc gcatcgacga tcacgtcctt gatctcacca
     2161 tccttcccgc ccgagagctg gacgttcttg ccgatgagat cgcttgccca gaaacgatca
     2221 ccttgttgct gggtcgcctg ccggtctgtt ccggcgctgc gagattgcac gccacgatct
     2281 gtcggagacg acgtgctctt tgcgctatcc gccgcgtacg cagcaccgct cactgccacg
     2341 cccgacgaga gcaatgccca ggcaatcgct cggctgatct tagtacgttc catactatcg
     2401 gacctccttt ctttgcaaag aaggttggaa tgacccgatt cgtctgagtt ccgatggatt
     2461 ttttcagctg tcgcccagga gtgacgggtg tcgtttcaca accacatggg taaaagcgcg
     2521 ttgtacacct tgccgtggag aggatgcgct atgacaacgt actgccacgt tggcggcgag
     2581 aagatgcgta atgcgttacc tcagcggcgc atcttctcgg ggagcaccgg gtgggtcggt
     2641 ttggtggagg ccgctccgga tccggtgccg ccacccgagg acgcgctctt gccgcccgac
     2701 acgccgctcg ccgaacgcgt gtccaccacc gcggcgccct cgcacggacc gtcctgataa
     2761 acgacggtgc cgttcggctg cttgcacttg aaaacctgcg ccgcggcggg gagtgggctc
     2821 ccgcagtaaa cggcggccgc aagcaaaccg agcagcattt ctcttttcat atcgacctcc
     2881 tggcgttatg cccgcgcaag tttgcgcgtc tcgaagcaat atgtcgaatg cgccaacttg
     2941 taacgcttac atgcgcgctg taagagttgc gcccgtctga cgccttgcgt cgcatagcaa
     3001 tgcatgcagc gacccggcat gaattatgct gcactcgcgc acgtttcaat gacgggtaga
     3061 tcacatggcc gagggcagcg cgaagttcca cgaggatgcc gagttgctga cacccgagac
     3121 gcgcgaccga caccgcgcca tcgcttccat catggaagag ctcgaggcgg tggactggta
     3181 cgatcagcgc gtggaagcaa cgcgcgatgc agagctgcgt gcgatcctcg cacacaatcg
     3241 cgacgaggaa aaagaacacg cggcgatggt gctcgagtgg ctgcgccggc gcgattcaca
     3301 cctcgatgag cagctccgca actatctttt catcgataag ccggtgctgg aacgcgagga
     3361 ggaactcgac caggccgaag cgggagctga tgcgccatcc gtgcccggag ccggcgaagg
     3421 ctc
//
LOCUS       DASURG010000455         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1305428, whole genome shotgun sequence.
ACCESSION   DASURG010000455 DASURG010000000
VERSION     DASURG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305428"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFY80_08020"
     CDS             <1..448
                     /locus_tag="VFY80_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX6007895.1"
                     /translation="WLGSTPYRWLEYTCWAYAAAYIILVPRMMRAVSRSDRRMLFRRR
                     DEQRGFTLQEVGLALLIVIAVAGLIMGTALIARVWGFYAGVGALVLWPLTLLVVPWYA
                     ALAHSDWLMLLVVHGGGLAGVHLYRTARVRGQVDASHIHDSVSEEP"
     gene            448..1248
                     /locus_tag="VFY80_08025"
     CDS             448..1248
                     /locus_tag="VFY80_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6007896.1"
                     /translation="MPQAVRRMLFIIVLLPLAAIAAEVSVRDIPTRAGVTQRVLLIQP
                     DRSIAAVILLPGGNGLVQIGADGSVGRGGNFLVRSREQFARAGFLVAVLDAPSDRIEG
                     PALDYFRQTDEHATDVGRVIMLLREEVKRPVWLVGTSRGTTSVANAAIRLRENGPDGV
                     VFTASLVGNDRERVPSMAIESIRAPALVVHHEEDACRYCLLRDVPALMVGLKGASRSE
                     LITFRGGGPARGNPCQAFHYHGFIGIEHEVVSRIADWIKSQLPSESGR"
     gene            1245..1568
                     /locus_tag="VFY80_08030"
     CDS             1245..1568
                     /locus_tag="VFY80_08030"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6007897.1"
                     /translation="MKQVHTAKHAAEAHLVKGFLESNGIEATVRGEYLTSGWGELPVD
                     LCSVWVNDDRQFERANELLIAFFKGTLARQYGGERWTCQHCGEQLEGQFTACWKCGTA
                     RPVDR"
     gene            complement(1714..2880)
                     /locus_tag="VFY80_08035"
     CDS             complement(1714..2880)
                     /locus_tag="VFY80_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6007898.1"
                     /translation="MRITAIHCATVPLASAIRNAYIDFSKMTASVVAVVTDAKRDGTR
                     VIGYGFNSNGRYAQGGLLAERFVPRLMSADPKALVDDAGVNLDPHRIWSCMMANEKPG
                     GHGERSVAVGVIDMAVWDAIAKLEGKPLYRVLAERYNVGESDDKVFVYAAGGYYYPGK
                     NLEALREEMKRYLDLGYTVVKMKIGGESLADDLRRIEAVIDVAGSGDRVAVDANGRFD
                     LKTALEYARALEPYGLFWYEEAGDPLDFELQAELARQYEKPLATGENLFSMQDARNLV
                     RYGGMRPDRDWLQFDCALSYGLVEYLRILAMLEKTGWSRRRCIPHGGHQMSLNIAAGL
                     GLGGNESYPDVFQPFGGFADDTPVRDGYVRLPDIPGVGFEAKKNLYNVMKSLAR"
     gene            2962..>3181
                     /locus_tag="VFY80_08040"
     CDS             2962..>3181
                     /locus_tag="VFY80_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007899.1"
                     /translation="MATPKRQSVSALTLQLSGYIAGAVRKAPPREVIARAKIHLVDTF
                     AAMISGSRLDPGQTALAYVRTLGGRPEAG"
BASE COUNT          550 a         1012 c         1020 g          599 t
ORIGIN      
        1 ctggctcggc agcacgccct accggtggct cgagtacacg tgctgggcgt acgctgccgc
       61 ctacatcatc ctcgttccaa ggatgatgcg ggccgtgtcg cgatcggacc gccggatgct
      121 tttccgacga cgggatgagc agcgcggatt cactctgcag gaagtagggc tcgcgctcct
      181 catcgtcatc gctgtagcag gcctcatcat gggcaccgcc ctcatcgccc gcgtatgggg
      241 tttttatgcg ggcgtcggcg cattggtgct ctggcctttg acgctgctcg tcgtaccgtg
      301 gtacgccgct ctcgcgcatt ctgactggtt gatgctcctc gtggttcacg gcggcggact
      361 ggccggcgtg catctctacc gcaccgcgcg cgttcggggg caggtcgatg catcgcacat
      421 ccatgactcg gtgagcgagg agccgtaatg ccgcaggcgg tgcggcgcat gctcttcatc
      481 atcgtgctgc tgccgctcgc tgcaatcgca gccgaggtat ccgtaagaga cattccgacc
      541 cgggcgggcg tgacgcagcg cgtgctgctc atccaaccgg accggtcgat cgcggccgtc
      601 attctgctcc ccggtggaaa cggtctcgtg cagatcggcg cggacgggag tgtgggacgc
      661 ggcggcaatt ttctggtgcg ttccagggaa cagtttgctc gggccggttt tctggtcgcc
      721 gtgctggacg cgccttcgga ccggatcgaa ggccccgccc tcgattactt ccgccaaacc
      781 gacgagcacg caacggatgt cggacgcgtc atcatgctcc tgcgcgaaga ggtgaaacgg
      841 ccggtgtggc tcgtcgggac gagccgcgga actacctccg tcgcgaacgc ggcgatccgg
      901 ttacgcgaga acggcccgga cggcgtcgtg ttcaccgcct cgctggtggg caatgaccgc
      961 gagcgcgtgc cgtccatggc aatcgaatcg atccgcgcgc ccgcgctggt cgtacaccat
     1021 gaggaggacg cgtgccgcta ctgcctcttg agagacgtgc cggcccttat ggtgggtctc
     1081 aagggcgctt ccaggtccga gttgatcacc ttcaggggcg gtggcccggc acgcgggaac
     1141 ccgtgccagg ctttccatta ccacgggttc atcggaatcg aacacgaagt cgtgtcccgc
     1201 attgccgact ggatcaagtc gcagctgccg agcgagagcg gccgatgaag caggtgcaca
     1261 cggcgaagca cgccgccgaa gcgcatctcg tcaagggatt tctcgagtcg aacgggatcg
     1321 aagcgaccgt gcgaggcgag tacctcacga gcggctgggg cgaattgccg gtcgatctgt
     1381 gctcggtctg ggtcaacgac gatcgccagt tcgagcgcgc caacgaactc ctgatcgcct
     1441 tcttcaaggg cacgcttgcg cgccaatacg gcggcgagcg ctggacgtgc cagcattgcg
     1501 gtgagcagct cgaaggtcag ttcactgcct gctggaagtg cggcaccgcg cgacccgtgg
     1561 atcggtgacg acaagcgtgg tcatcggcca catgcgttct ggattgcggc tttcgccgca
     1621 atgacgttga atcgtcatgc cggtgaaaac cggcatccag caaagcatct gtctctggct
     1681 tccggcattc gccggaatga cggcagaaga aactcagcgc gcgagcgact tcatgacgtt
     1741 atagaggttt ttcttcgctt cgaagccgac cccaggaatg tcaggcaatc tcacgtagcc
     1801 atcgcgaacc ggagtgtcgt ctgcaaaacc gccgaacggc tggaagacat cgggatacga
     1861 ttcattcccg ccgagcccga ggcccgccgc gatgttgagt gacatctggt gcccgccgtg
     1921 cgggatgcag cggcggcgcg accacccggt tttttcgagc atcgcgagta tgcgcagata
     1981 ttcgacgagc ccgtacgaga gcgcgcagtc gaactgcaac cagtcgcgat cgggccgcat
     2041 gccgccgtag cggacgaggt tgcgcgcgtc ctgcatcgag aagagattct cgccggtcgc
     2101 aagcggcttt tcatattggc gggcaagctc cgcctgcagc tcgaagtcga gcggatcgcc
     2161 cgcctcctcg taccagaaaa gcccgtaggg ctcgagcgct cgcgcatatt cgagcgcggt
     2221 tttcagatcg aagcgtccgt tggcatcgac cgcaactctg tcacccgacc ccgccacatc
     2281 gatcaccgct tcgatgcgcc ggagatcgtc ggcgagcgac tcgccgccga tcttcatctt
     2341 gacgacggtg tagccaagat cgagataccg cttcatctct tcccgcagcg cttcgaggtt
     2401 cttgcccgga tagtagtagc cgcccgccgc atagacgaac accttgtcgt cgctttcgcc
     2461 cacgttgtag cgctcggcga gcacgcgata gagcggtttc ccttcgagct tggcgattgc
     2521 atcccacacc gccatgtcga tcacgccgac cgcgaccgag cgctcgccgt ggccacccgg
     2581 tttttcattc gccatcatgc atgaccagat gcggtgcgga tcgaggttca cccccgcgtc
     2641 gtccaccagc gccttcggat cggcgctcat gaggcgcggg acgaagcgct ccgcgaggag
     2701 cccgccttgc gcgtagcgac cgttcgagtt gaagccgtag ccgatgacgc gcgtgccgtc
     2761 gcgcttggca tcggtcacga ccgccacgac gctcgcggtc atcttcgaga aatcgatgta
     2821 ggcgttgcgt atggccgaag cgagcggaac ggtcgcgcag tgaatcgcgg tgattctcat
     2881 gggcttgaag tcaatgggat ggatggcgtg gcgccgtgtt gtaacatgtg ttgtcgttgc
     2941 tcacaactcc agggagcccg tatggcaacg ccaaaacgcc agagcgtttc cgcgctgact
     3001 ctgcagctca gcggctacat cgcgggcgcc gtgcgcaagg caccgccgcg ggaagtgatc
     3061 gcgcgcgcaa agattcatct cgtcgacacg ttcgcggcaa tgatctctgg ctcgcgcctc
     3121 gatcccggtc agaccgcact cgcctacgtg cgcacgctgg gcggcaggcc ggaagccggc
     3181 c
//
LOCUS       DASURG010000456         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_693036, whole genome shotgun sequence.
ACCESSION   DASURG010000456 DASURG010000000
VERSION     DASURG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693036"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFY80_08045"
     CDS             <1..216
                     /locus_tag="VFY80_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6007900.1"
                     /translation="KGKPLPPWAADFDCTGWAQFFLKYLLGHPAVTCVIPGTRRVAHL
                     KDNMQAGMGRLPDAATRKRMVEYLGRL"
     gene            280..765
                     /locus_tag="VFY80_08050"
     CDS             280..765
                     /locus_tag="VFY80_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6007901.1"
                     /translation="MTTIKAMTRVFSITAATLFAAVALAQDQRPGYGTAVNLATAKKM
                     AAGALAECQKNKWNVAVAVVDNHGLLLYFERLDDTQTASSQIAVDKARAAAMYRRPTR
                     AFADVIAKTGPAVMTLPGVVASPGGLPIFAGGRVVGGIGISGVTGDQDEQCAKAGIAG
                     M"
     gene            903..1814
                     /locus_tag="VFY80_08055"
     CDS             903..1814
                     /locus_tag="VFY80_08055"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEX6007902.1"
                     /translation="MTPFRTFQILLRLSALACCLGVLASCSMIRLGYGHLDTYAAWKA
                     HEYFDLDAHQKDEFLRRFNRLHEWHRYEQLPDYAAFLATARKRLDKPLHSEDVDWFID
                     GVKVRYARVVSHATGDAVALLYTITPAQLEALRRQWEKDSRRFVREYRLTGSAEDIKR
                     ARARRTLEQVREWVGALSPEQEARIVSMSDALPMTEKLRHEDRVRRQREFLQLMAQRG
                     EDRKAFAERLGRWLIDWDKGRAPEYERRSREWYAQRVAMVIEIERMLTPHQRAVVLGR
                     IDDYIADFARLGQQPPSRTASNQALLQ"
     gene            1848..>2146
                     /locus_tag="VFY80_08060"
     CDS             1848..>2146
                     /locus_tag="VFY80_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6007903.1"
                     /translation="MPAYSRPIASKLPRVGTTIFTVMSRLAAEHNAINLSQGFPDFDC
                     APELRALLVKHLNAGVNQYPPMAGITALREAVAEKAEALYGVRYDAEHEVTIVPGA"
BASE COUNT          383 a          719 c          695 g          349 t
ORIGIN      
        1 aagggcaaac cgctgccgcc gtgggccgcc gacttcgact gcacgggctg ggcgcagttt
       61 ttcctcaaat atctgctggg ccacccggcg gtgacctgcg tcatccccgg aacccggcgc
      121 gtggcgcatc tcaaggacaa catgcaggcc ggcatgggcc ggctgccgga cgcggcgacc
      181 cggaagcgga tggtggaata tctggggcgg ctatgacgtt agcgtagcga gccggccgcg
      241 gtgtcggccg cgtttccacc acccaccatg gaggctgcaa tgacaacgat caaagcaatg
      301 acccgcgtct tttcgattac ggccgcgacg ctcttcgccg cggttgcgct cgcacaggat
      361 cagcggccgg gatacggcac ggccgtcaac ctcgccacgg cgaaaaaaat ggcggcgggc
      421 gcgctcgccg aatgccagaa gaacaagtgg aacgtcgccg tggccgtcgt ggacaatcac
      481 ggcctgctcc tctacttcga gcggctggac gacacccaga cggccagctc gcagatcgcc
      541 gtggacaagg cgcgggccgc agccatgtat cggcgcccga cccgtgcatt cgcggatgtg
      601 atcgccaaga ccggcccggc cgtcatgacc ctgcccggcg tcgtcgcctc cccgggaggc
      661 ctgccgatct tcgccggcgg cagagtcgtc ggcggcatcg gaatcagcgg cgtcacggga
      721 gaccaggacg agcaatgcgc caaagcggga attgccggga tgtgaacacg ctccgctcct
      781 gtggcacacg gaacggggct gcggccccgt ttttttttgc cggaactacg gctgcgaatg
      841 agggtccctt ggcgaggcgc cctgcggtat ttttccgaag cgccccgcta ctagatatcc
      901 ctatgacgcc tttccgaact ttccagattc tcctgcgttt gagcgcactc gcctgctgcc
      961 tcggcgtgct cgcaagctgc agcatgatac gcctgggcta cggccatctc gacacttatg
     1021 cggcgtggaa agcccacgag tatttcgatc tcgacgccca ccagaaggac gagtttctcc
     1081 ggcgtttcaa ccgcctgcac gagtggcatc gttacgagca acttcccgat tatgccgcct
     1141 ttctcgccac cgcccggaag cgcctcgaca agccgctcca cagcgaggac gtggattggt
     1201 tcatcgacgg cgtcaaggtg cgttatgcgc gcgtcgtgtc tcacgcgacc ggcgatgccg
     1261 tggcgctcct gtacaccata acgccggcgc agctcgaagc gctgcgccgg cagtgggaaa
     1321 aggacagccg ccgattcgtt cgcgagtacc gccttaccgg cagcgcggag gatatcaagc
     1381 gcgcccgcgc gcgccgcacg ctggaacagg tccgggagtg ggtcggcgcc ctgagccccg
     1441 agcaggaggc gcggatcgtc tcgatgtcgg acgcgctgcc gatgacggaa aagctgcgcc
     1501 atgaagaccg cgtgcgccgc cagcgggaat tcctgcagct catggcgcag cgcggcgagg
     1561 atcgcaaggc gttcgcggag cggctcggga ggtggctcat cgactgggac aagggccgcg
     1621 ctccggaata cgagcgccgt tcgcgcgagt ggtacgcgca acgggttgcg atggtaatcg
     1681 agatcgagcg catgctgacg ccgcatcagc gcgccgtcgt gctcggccgc atcgacgatt
     1741 acatcgcgga cttcgcacgg cttgggcagc agcccccgtc gcggaccgcc tcgaaccagg
     1801 cgctgctaca ataacgcgcg atcccggagg aatcaccgaa gtcagccatg cctgcctact
     1861 cgcgtcccat cgcgtcgaaa ctgccgcggg tcggcaccac cattttcacc gtcatgtcgc
     1921 ggctcgccgc cgagcacaac gcgatcaatc tttcgcaggg ctttcccgac ttcgattgcg
     1981 cccccgagct gcgcgcgctg ctcgtcaagc acctcaacgc cggcgtcaat caatatccgc
     2041 cgatggccgg catcacggcg ctgcgcgaag cggtcgccga aaaggccgag gcgctgtacg
     2101 gcgtgcgtta cgacgcagag catgaggtaa cgatcgtgcc gggcgc
//
LOCUS       DASURG010000457         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1305932, whole genome shotgun sequence.
ACCESSION   DASURG010000457 DASURG010000000
VERSION     DASURG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305932"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFY80_08065"
     CDS             complement(<1..372)
                     /locus_tag="VFY80_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6007904.1"
                     /translation="MSETIEFAPSLRSMLDRMLLIRAYEEKAVAMQAAGQAAGTCTAV
                     GQEASAVGVVTALGSRDLILTNHRSAGHLLARGADPARLYAEVLGKVTGYCKGKSGSL
                     HISVKELGVVLTSTIVGAELSL"
     gene            complement(392..892)
                     /locus_tag="VFY80_08070"
     CDS             complement(392..892)
                     /locus_tag="VFY80_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6007905.1"
                     /translation="MRIIYTFSAFCLAAILSATASAASPDDKATLARGKYLVATSGCN
                     DCHTAGYPESGGRVPEAEWLTGSPVGFTGPWGTTYPANLRLTVQALSEDAWMMRARSE
                     LRPPMPWFSLRDMTDSDLRAMYRYIRSLGPKGGMAPAYVPPGSKVATPYIEFVPKNLP
                     ARAAVK"
     gene            complement(1039..2154)
                     /locus_tag="VFY80_08075"
     CDS             complement(1039..2154)
                     /locus_tag="VFY80_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007906.1"
                     /translation="MSGLNDFLKRPGALIECDDDPEAVFELLCEKGWSDGLPVIPPTP
                     ERVERMLAYCDRDPDKPVVKIPPRYGAATPIRIAANAVMAGCKPEHFPLVVLALEAMA
                     EEPYNLYGTQATTHPCTPFLIFNGPIARELGINCGHNVMGNYFRPNAVIGRAVRLALV
                     NIGAAIPGIGDMATHGTPAKFTYCVAENEAASPWEPLHVELGFPKEATTVTVVAGEGP
                     HNINDHESLTPEGILTMCAGTIATTGSNNAYYRGQPVLAFGPEHAATVASRGMSKREV
                     KHWLYEHATIPLSKFSKEGIERRFRRKQSEEFENAPLDTPVHMWKTPEDLIVIVTGGA
                     GKHSAYVPTFGNTRAVTRALKHASGVLVNAILELKRN"
BASE COUNT          385 a          744 c          743 g          468 t
ORIGIN      
        1 gagcgaaagc tcggcaccga cgatggtgga ggtcagcacg accccgagtt ccttcaccga
       61 aatatggagc gagccgctct tgcccttgca gtacccggtc accttgccca gcacttcggc
      121 ataaagccgc gcgggatcgg cgccgcgcgc cagcaggtgt ccggcgctgc ggtggttggt
      181 aaggatgaga tcgcggctgc cgagcgcggt gacgacaccg acggcgctcg cttcctgtcc
      241 gactgcggtg caggtgccgg cggcctggcc tgcggcctgc atcgcgaccg ccttttcctc
      301 gtaggcgcgg atcaggagca ttctgtcgag catggaacgc aggctcggcg caaactcgat
      361 cgtttctgac atgtgaatcc tttccggagt gttatttcac ggcggcccgt gccggcagat
      421 tcttcggcac gaactcgatg tagggtgtcg caactttgct gcccggcggc acgtaggccg
      481 gcgccatgcc acctttgggc ccgagactgc ggatgtagcg atacatcgcg cgcaaatcgg
      541 agtccgtcat gtcacgcagc gagaaccacg gcatgggcgg acgcagctcc gaccgagccc
      601 gcatcatcca ggcgtcttcg gacagcgctt gcaccgtcaa acgcaagttc gcaggatagg
      661 tcgtacccca gggccccgtg aacccgaccg ggctgccggt cagccattcg gcttccggta
      721 ccctgccgcc cgattcggga tagccggccg tgtggcagtc gttgcagccg ctcgtggcga
      781 cgagatactt gccgcgcgca agcgtagctt tgtcatcggg tgacgcggcg ctggccgtcg
      841 cactcagaat cgctgcaagg cagaacgcag aaaaagtgta gatgatgcgc accgtcttct
      901 cctctcttgt gaattgacga gagaatgata ggaagacgcg tgctcggtgt ttgctaaatg
      961 atgcttatgc gaatcctaaa tttttcgtaa gcgatgcacc ttgtgttgca gaggtagcgc
     1021 gggcgtatcg gctttgtttc agtttcgttt caattcaaga atcgcgttca ccagtacacc
     1081 gctcgcgtgc ttcaatgcgc gcgtcacggc gcgcgtgttg ccgaaggtcg gcacgtaagc
     1141 ggagtgcttg cccgcgccgc cggtcacgat gacgatcagg tcctcgggtg tcttccacat
     1201 gtgcaccggc gtgtcgagcg gcgcgttttc gaactcttcc gactgcttgc ggcgaaaacg
     1261 gcgctcgatg ccctccttcg agaacttcga aagcggaatg gtcgcgtgct cgtagagcca
     1321 gtgcttcacc tcacgcttcg acatgccgcg gctcgcgacg gtcgccgcgt gctcggggcc
     1381 gaatgcgagg acgggctgcc ctctgtagta agcgttgttg gatccggtcg tcgcgatcgt
     1441 ccccgcgcac atcgtgagaa tgccttcggg cgtgaggctc tcgtgatcgt tgatgttgtg
     1501 cggcccctcc cccgccacga ccgtcaccgt ggttgcctct ttcgggaaac cgagctcgac
     1561 atgcagcggc tcccacgggc ttgccgcttc gttctccgcg acgcagtagg tgaatttcgc
     1621 gggcgtgccg tgggtcgcca tgtcgccgat gccgggaatc gccgcgccga tgttgacgag
     1681 cgcgagcctc accgcacggc cgatgacggc gttcgggcga aagtaattcc ccatgacgtt
     1741 gtgcccacaa ttgatgccga gttctcgcgc gatcggcccg ttgaagatga ggaacggcgt
     1801 gcacgggtgc gtggtggcct gcgtgccgta gaggttgtac ggctcctcgg ccatcgcttc
     1861 gagtgcgagc acgacgagcg ggaaatgctc cggcttgcag cccgccatca ccgcgttcgc
     1921 agcgatgcgt atcggcgtcg ccgcgccgta gcgcggcgga atcttcacca ccggcttgtc
     1981 gggatcgcgg tcgcagtagg cgagcatgcg ctccacgcgc tcgggcgtgg gcggaatcac
     2041 cggcaggccg tcgctccagc ccttctcgca cagaagctcg aaaacggctt cgggatcgtc
     2101 gtcgcactcg atcagcgcac cgggccgctt caaaaaatcg ttgaggccac tcacgtcgcc
     2161 gcctccgtta gctcgactac acatcgttcg ccttcatgtc ggcagcaagc gcccttcgcg
     2221 ggtcgcatcc ccgtaaacgg ggcccctgct ccgccgctca tgtcgctttc tcccggatca
     2281 ctttgaagag ttgcggcagc gcgacttgcg cccgttcgcg gactttgtcc atgccgagcc
//
LOCUS       DASURG010000458         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1409228, whole genome shotgun sequence.
ACCESSION   DASURG010000458 DASURG010000000
VERSION     DASURG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409228"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFY80_08080"
     CDS             complement(<1..193)
                     /locus_tag="VFY80_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6007907.1"
                     /translation="MTIVADARRILVACFVACAGGAAAASAQDYPVKPIRMVVPFPPG
                     GFSDVFARIIGGKMNESWGQ"
     gene            complement(526..1269)
                     /gene="surE"
                     /locus_tag="VFY80_08085"
     CDS             complement(526..1269)
                     /gene="surE"
                     /locus_tag="VFY80_08085"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX6007908.1"
                     /translation="MRILVSNDDGVYSPGIAALAEAASDFGEVRVVAPDVEMSSAGHS
                     ITAWRPLSYKHTPLGGMEAYRVNGTPADCVALGTSQWEGIDIVLSGINLGSNVGHGLW
                     HSGTLAAAKQAVLMGLRGVALSIPTPKDQPDFKKLRPAVREVLGLLLPDESLSLVNVN
                     FPHEQPKGMMWTRQSIRLYDGKVMPGEDPMGRKHYWFTVVPVTETDEGTDRWALERGY
                     ISITPLRLDLTDEEKLRAMQARHPLRAVR"
     gene            1317..>2170
                     /locus_tag="VFY80_08090"
     CDS             1317..>2170
                     /locus_tag="VFY80_08090"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6007909.1"
                     /translation="MQVLLVHNPDAGDKSAPDEKALKKLIREAGHEVEYCSIRGQKWK
                     RVIADAPDLVAVAGGDGTVVQVARELIGRQVPIAVLPLGTANNISKSLGVKHLPIEDI
                     VASWNAAQHKRFDAGVISAPWGKRYFVEAAGAGLFALAMPEADANPTLENMEDAEARA
                     TYAQQILRERLKACRPLHLDITADEKTISGEYILFEAMNMEYVGPNLYLAPDSVGDDG
                     RLDVVLIAERQRRKLDRYLETWQDGVARPPDFDALKAERIELQWTGFDMHVDDALFPA
                     KKRRPPQRP"
BASE COUNT          405 a          686 c          690 g          389 t
ORIGIN      
        1 gctggcccca ggattcgttc atctttccgc cgatgatgcg cgcgaagacg tcagagaatc
       61 cgcccggcgg aaacggcacg accatccgta tcggcttgac aggatagtcc tgcgcactcg
      121 ccgcggctgc gccgccggcg caggcaacga aacacgcgac gagaatacgt ctcgcgtcgg
      181 caacgatagt catcgatgtt ctcccttgga acgcggcacg gcctgagcga gccttctcgc
      241 tcgctcaggc gccgctgcgc ccggcctgat cagattatag cgtcagtgtc tgttcgacgc
      301 gcgtgccgga tgtgtactct gcgaagcgtc ccgaatgcgt cagcgcaagc cgaagaacga
      361 gagtatcgcg atgatgacta cgatcaagcc gatgatatag atgatgttgt tcacgaagct
      421 tcctttgcgt tgacgagcgc cggagcgctg cgtgctggat ggtgcagcga agccttttgc
      481 gagcagtcgg gggtgtgacc acgtggctgc gtcaaagttg ccggcctagc ggacggcgcg
      541 gagcggatgc cgtgcctgca tcgcgcgcaa cttttcttca tcggtgagat ccagccgcag
      601 cggcgtgatg gagatgtagc cgcgctccag cgcccagcgg tccgttccct cgtcggtctc
      661 ggtcaccggc accaccgtaa accagtagtg cttgcgcccc atcggatcct cgcccggcat
      721 caccttgccg tcgtagaggc ggatcgactg tcgtgtccac atcatgccct tcggctgctc
      781 gtgcggaaaa ttgacgttga cgagcgacag cgactcatcg ggcagcagca gcccgagcac
      841 ttcccggacc gcgggcctga gtttcttgaa atccggctga tccttggggg tcgggatgct
      901 gagcgcgact ccgcgcaaac ccatcagtac cgcctgtttt gccgcggcga gcgtgccgga
      961 gtgccagagg ccgtggccga cgttgctgcc aaggttgatg cccgagagca cgatgtcgat
     1021 gccttcccac tgtgaggtgc cgagcgccac gcagtctgcc ggcgtaccgt tcacgcgata
     1081 cgcttccatg cctccgagtg gcgtgtgctt gtagctcagc ggccgccatg ccgtgatcga
     1141 atggcccgcg gaggacatct ccacgtccgg cgcaacgacg cgcacctccc cgaaatccga
     1201 cgccgcttca gcgagcgccg cgatgcccgg gctgtagacg ccgtcgtcgt ttgaaaccag
     1261 tatccgcatg gccgtgtctc atgtttggtt tcatttttgg gggagggttc catccgatgc
     1321 aggtgctgct ggtgcacaac ccggatgccg gcgacaagtc cgcgccggac gagaaggcgc
     1381 tgaagaagct catccgcgag gcgggacacg aggtcgaata ctgttccata cgggggcaaa
     1441 aatggaagcg tgtcatcgcg gatgcgccgg accttgtcgc ggtcgcgggc ggcgacggca
     1501 cggtggtgca ggtcgcgcgc gagctgatcg gccggcaggt tccgatcgcg gtcctgccgc
     1561 tcggcaccgc caacaacatt tcgaaatcgc tcggcgtcaa gcacctgccg atcgaggaca
     1621 tcgtcgcgag ctggaacgcc gcccagcaca agcgcttcga cgcaggtgtc atatcggcac
     1681 cgtggggcaa gcgttacttc gtcgaggctg caggcgccgg gctcttcgca ctcgccatgc
     1741 cggaggcgga tgcgaatccc acgctggaaa acatggagga cgctgaagcg cgcgcgacct
     1801 acgcacagca gatacttcgg gagcggctga aggcctgccg gccgctgcac ctcgacatca
     1861 ctgcagacga aaagacgatc tccggcgagt acatcctttt cgaagcgatg aacatggaat
     1921 acgtcggtcc taatctctac ctcgcacccg acagcgtcgg cgacgacggc cggctcgacg
     1981 tggtactcat cgcagaacgc cagcggcgca agctcgaccg gtacctcgaa acgtggcagg
     2041 acggtgtcgc gcggccgccc gatttcgacg cgctcaaagc cgagcgtatc gaacttcagt
     2101 ggacaggctt cgacatgcac gtggacgatg cgttgtttcc cgctaaaaaa agacgtccgc
     2161 cacagcgacc
//
LOCUS       DASURG010000459         4956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_999940, whole genome shotgun sequence.
ACCESSION   DASURG010000459 DASURG010000000
VERSION     DASURG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999940"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VFY80_08095"
     CDS             complement(<1..1427)
                     /locus_tag="VFY80_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX6007910.1"
                     /translation="MSSIQDPGSGTLQLPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     TAMESGKSIVLVAQKSAAKDEPAPEDLYGVGSIANILQMLKLPDGTVKVLVEGNQRAR
                     INEILDVKTHFAVNVTPVAFDQTTDHEVEAMRRTMIQQFDQYVKLNKKIPPEILTSLA
                     GIDEAGRLGDTIAAHLPLKLEQKQEVLEMFGVKERLEHLLKLVEGELDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGELEEGADLDEIEKRIKSARMPKEARKKAESE
                     LKKLKLMSPMSAEATVVRNYIDAVVSLPWRKRSKISTDLGAAEKVLDEDHYGLEKVKE
                     RIVEYLAVQQRVDKLKAPILCLVGAPGVGKTSLGQSIARATNRKFVRMSLGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMTKVGVRNPLFLLDEVDKMGMDFRGDPASALLEVLDPEQ
                     NHTFVDHYIEVEYDLSDVMFVATANT"
     gene            complement(1546..2814)
                     /gene="clpX"
                     /locus_tag="VFY80_08100"
     CDS             complement(1546..2814)
                     /gene="clpX"
                     /locus_tag="VFY80_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6007911.1"
                     /translation="MSDKVGGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREETQTEHGGKSAKSDLPVPHEIRAILDQYVIGQDVAKKILSVAVYNHYKRLKSVA
                     KGDDVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVENI
                     IQKLLQKCDYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTI
                     ASVPPQGGRKHPNQEFVQVDTTNILFVCGGAFDGLDKIIRARSEKSGIGFAAEVSSRD
                     NRKDITKVLREVQPEDLIKYGLIPEFVGRLPVVATLEELDEVALIQILTQPKNALLKQ
                     FQKMFGMEGVELEVREPALRAVAKRALERKTGARGLRSILEHTLLNTMFDLPSMENVV
                     KVVVDEGTIVSDAAPLLIYSDQPKVAGSTV"
     gene            complement(2850..3473)
                     /gene="clpP"
                     /locus_tag="VFY80_08105"
     CDS             complement(2850..3473)
                     /gene="clpP"
                     /locus_tag="VFY80_08105"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX6007912.1"
                     /translation="MKQYMEPQGLGLIPMVIEQSGRGERAYDIYSRLLKERVVFLVGP
                     VTEVTANLIVAQLLFLESENPDKDISFYINSPGGMVSAGLAIYDTMQFIKPDVSTLCI
                     GQAASMGALLLAAGAKGKRFCLPNSRVMIHQPMGGFQGQASDVEIHAREILFLKGRLN
                     EILARHTGQKIETIQKDTDRDNFLSADQAVSYGLVDRVLVSRTETVA"
     gene            complement(3470..4780)
                     /gene="tig"
                     /locus_tag="VFY80_08110"
     CDS             complement(3470..4780)
                     /gene="tig"
                     /locus_tag="VFY80_08110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6007913.1"
                     /translation="MQANLETLGQLERRLSVAVPMAEIDSEIESRLKKLTRSVKMHGF
                     RPGKVPLKVVEQQYGPQVRQEVLGDTVEKSFGEAVRQQKLKVAGYPKFEAKPLEAGAS
                     AFEYSATFEVYPEIRVGDLSQVTLTRPQLEVGEAEVDKTLEVIRKQRATYEPVDRGAE
                     PGDRLNIDYRGTIDGTDFEGGTAQGHNVTLGEGRLLPDFDAQLKGMASGDARTFDVRF
                     PDDYHGKDVAGKTARFDVAVKAVAAPRLPELNADFARQLGVGDGDLGKMRAEVKANLE
                     REVKLRLKNRVKEQVMQALLQVTQFDAPKSLVDLEVQRLQSRMRQELAASGLPVKNET
                     PMPREVFEQQAERNVRLGLILGELVRAQNLQPRPEQVRAVVEEQAQSYERPAEVVKWF
                     YQSPERLRDIEAAVMEDNVVEWALKTARNEDKAMAFDELMGTRQ"
     gene            complement(4811..4895)
                     /locus_tag="VFY80_08115"
     tRNA            complement(4811..4895)
                     /locus_tag="VFY80_08115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4859..4861),aa:Leu,seq:tag)
BASE COUNT          833 a         1647 c         1414 g         1062 t
ORIGIN      
        1 gtgttcgctg tcgccacgaa catcacgtcc gacaggtcgt actcgacctc gatgtagtgg
       61 tcgacgaagg tgtgattctg ctcgggatcg agcacttcga gcagcgcgga cgcgggatcg
      121 cctcggaaat ccatgcccat cttgtccact tcgtcgagca ggaagagcgg attgcgaacg
      181 ccaaccttcg tcatgttctg caggatcttc ccgggcatcg agccgatgta ggtgcggcgg
      241 tgcccgcgaa tctccgcttc atcgcgcacc ccgccaagcg acatgcgcac gaacttgcgg
      301 ttcgtcgcgc gcgcgatcga ctgtcctagc gaggtcttgc ccacgccggg agcgccgacg
      361 aggcacagga tcggcgcctt cagcttgtcc acgcgctgct gcaccgcgag atactcgacg
      421 atccgttcct tcaccttctc gagcccatag tggtcttcgt cgagcacctt ttcggccgcg
      481 ccgaggtcgg tgctgatctt gctgcgcttg cgccacggca acgacacgac tgcatcgatg
      541 tagttgcgca cgaccgtcgc ttccgcggac atgggggaca tgagcttcaa cttcttcagc
      601 tcgctctccg ccttcttgcg cgcctccttc ggcatgcggg ccgatttgat gcgcttttcg
      661 atctcgtcga ggtccgcgcc ttcttcgagc tcgcccagct ccttctggat cgccttcacc
      721 tgctcgttca ggtagtactc gcgctggctc ttctccatct ggcgcttcac acgcccgcgg
      781 atgcgttttt cgacctgcag gatgtcgagt tccccttcca cgagcttcaa gaggtgctcg
      841 aggcgttcct tcacgccgaa catctccagc acttcctgct tctgttcgag cttgagcggc
      901 agatgcgcgg caatcgtgtc gccgagccgg cccgcctcgt cgatgcccgc cagggaagtg
      961 aggatctccg gcggaatctt cttgttcaac ttcacgtact ggtcgaactg ctggatcatc
     1021 gtgcgccgca tcgcctcgac ttcgtggtcg gtcgtctggt cgaacgccac gggtgtgacg
     1081 ttgaccgcga aatgcgtttt cacgtcgagg atttcgttga tgcgcgcgcg ctggttgccc
     1141 tcgaccagca ccttcaccgt gccgtccggg agcttgagca tctgcaggat gttggcgatg
     1201 ctgccgacgc cgtagaggtc ctcgggcgcg ggctcatcct tcgccgcgga tttttgcgcg
     1261 accaacacga tgctcttgcc cgattccatc gccgtctcga gcgccttgat cgacttcggg
     1321 cgtccgacga aaagcgggat caccatatgg ggaaatacga cgacgtccct cagcggcaac
     1381 agcggaagct gcaacgtgcc ggaaccgggg tcttgaatgc tggacatggg ggtcatttcc
     1441 tcaaatcgcc tggaaaggca tgaaaaatta acattgggcg cgccgccgcg aaatcaagcg
     1501 acgcccccgc aacgattccg ccgggggccg cgcggcatcg gatgactata ccgtggatcc
     1561 agccaccttc ggctggtccg agtaaatgag aagcggcgcc gcgtcgctca cgatggtgcc
     1621 ctcatccacg acgaccttga cgacgttctc catcgacggc aggtcgaaca tggtgttgag
     1681 cagcgtgtgc tccagtatgg agcgcaggcc gcgcgcgccc gtcttgcgct cgagcgcgcg
     1741 cttcgcgacc gcgcggagcg caggctcgcg cacttcgagc tccacgcctt ccatgccgaa
     1801 catcttctgg aactgcttga gcagcgcgtt cttgggttgg gtcaggatct ggatgagcgc
     1861 cacctcgtcc agctcttcga gcgtcgcgac aacgggcagg cgtccgacga actcggggat
     1921 caggccgtac ttgatgagat cctcgggctg cacctcgcgc agcaccttcg taatgtcctt
     1981 gcggttgtcg cggctgctca cctcggcggc aaagccgatg ccgctctttt ccgagcgcgc
     2041 gcggatgatc ttgtcgagcc cgtcgaacgc cccgccgcac acgaaaagga tgttggtcgt
     2101 atcgacctgc acgaactcct ggttgggatg cttgcgcccg ccctgtggcg gcaccgaagc
     2161 gattgtcccc tcgatcagct tgagaagcgc ctgctgcacg ccctctccgg acacgtcccg
     2221 tgtgatcgaa ggattgtcgg acttgcggga aatcttgtcg atctcgtcga tgtagacgat
     2281 gccgcgctgg gctttctcga cgtcgtaatc gcacttctgc agcagcttct ggatgatgtt
     2341 ctccacgtcc tcgccaacgt atccagcctc cgtcagcgtc gtcgcgtccg ccatcacgaa
     2401 cggcacgttg agcagccgcg cgagcgtctg cgcgaggagt gtcttgcccg aaccggtcgg
     2461 gccgatcagg aggatgttcg atttcgccag ttcgacatcg tcgcccttgg cgacgctttt
     2521 caggcgcttg tagtggttgt acaccgccac ggacagaatt tttttcgcga cgtcctgacc
     2581 gatgacgtac tggtcgagga tcgcacggat ctcatgcggc acaggcaggt ctgacttggc
     2641 gctcttgcca ccgtgctcgg tctgcgtctc ttcgcgaatg atgtcgttgc agagctcgat
     2701 gcattcatcg cagatgaata ccgacggtcc ggcgatcaac ttcctcacct cgtgctggct
     2761 cttgccgcag aacgaacagt agagcagttt ctcgccgcca accttgtctg acataccgct
     2821 tttcctcatc tggacacagg tttaggacac tatgcaacgg tttccgttct actcacgagc
     2881 acgcggtcga cgaggccata gctcacagcc tgatccgcag agagaaaatt gtcgcgatcc
     2941 gtgtccttct ggatcgtctc gattttctga ccggtgtgcc gcgcgagaat ctcgttcaac
     3001 cggcccttga ggaaaagaat ctcgcgcgca tggatctcga catccgaagc ctgtccctgg
     3061 aaaccgccca tcggctgatg gatcatcacg cgcgaattgg gcaggcagaa gcgcttgccc
     3121 tttgcgcccg ccgcaagcag cagcgcgccc atgctcgccg cctggccgat acagagcgtc
     3181 gagacgtccg gcttgatgaa ctgcattgtg tcgtagattg caagccccgc ggacaccatt
     3241 ccgcccgggg aattgatgta gaacgagatg tccttgtccg ggttctccga ttcgaggaaa
     3301 aggagctgcg cgacgatcag gttcgccgtc acctccgtca ccggcccgac cagaaacacc
     3361 acgcgctcct tcagcaggcg cgagtagata tcgtaggcgc gctcgccgcg gccgctctgc
     3421 tcgatcacca tggggatcaa gccgagaccc tgaggttcca tgtactgctt cattgtcgtg
     3481 tacccattaa ttcgtcaaag gccatcgcct tgtcttcgtt gcgggcggtc ttcagcgccc
     3541 actcgacgac attgtcttcc atcaccgccg cctcgatgtc gcgcaagcgc tccggcgatt
     3601 gataaaacca cttcaccacc tcggcgggac gctcatagct ttgcgcctgt tcctcgacca
     3661 ctgcccgcac ctgctccggc cgcggctgca ggttctgcgc ccggaccaac tcgcccagga
     3721 tgagacccag tcgcacgttg cgctccgcct gctgctcgaa gacttcccgc ggcatcggcg
     3781 tctcgttctt gaccgggagg ccgctcgccg cgagctcctg ccgcatccgg ctctggagcc
     3841 gctgcacctc caggtccacg agcgacttcg gggcatcgaa ctgcgtcacc tgcaggagcg
     3901 cctgcatcac ctgctccttc acccggttct tgagccgcag cttcacttcg cgctcgaggt
     3961 tcgccttgac ttcggcccgc atcttgccga gatcgccatc cccgactcca agctggcgcg
     4021 caaagtcggc gttgagttcc ggcagccgcg gcgccgcgac cgctttcacc gccacatcga
     4081 accgggccgt cttgcccgca acgtccttgc catgatagtc atccgggaag cgcacatcaa
     4141 acgtcctcgc gtccccgctt gccatccctt tcagctgagc atcgaaatcg gggagcagcc
     4201 gaccttctcc cagggtgacg ttatgaccct gagcggtgcc gccttcgaag tctgtaccgt
     4261 cgatggtacc gcggtagtcg atgttcaggc gatcgccggg ctccgcgccg cgatccaccg
     4321 gctcatacgt ggcccgctgc ttccgtatga cctcgagcgt cttgtcgact tccgcttccc
     4381 cgacctcgag ctggggccgc gtgagagtca cctgggacag atcacctacc cgtatctcgg
     4441 gatagacctc gaacgtagcg ctgtactcga aagcactcgc acccgcttcg agcggttttg
     4501 cctcgaactt gggatagccc gccaccttca gcttttgctg ccgcaccgct tccccgaagc
     4561 tcttctcgac tgtgtcgccc agaacttcct gtcgcacttg ggggccgtac tgctgttcca
     4621 ccactttgag tggcaccttg ccgggacgaa agccgtgcat cttcaccgat cgtgtgagct
     4681 ttttaagccg cgactcgatc tcgctgtcga tctcggccat cggtaccgct acgctcaggc
     4741 ggcgttcgag ttgccccagc gtttcgaggt tcgcttgcat taaatcagga tcccctataa
     4801 ccgtgtcatc tggtgcgaaa ggagggactc gaacccacac gcctttcggc gccagaacct
     4861 aaatctggtg cgtctaccaa ttccgccact ttcgcacggc cgtaaagccg gaccgccgga
     4921 tccgggttca gggccccgag gatccggctt taccgc
//
LOCUS       DASURG010000460         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_387454, whole genome shotgun sequence.
ACCESSION   DASURG010000460 DASURG010000000
VERSION     DASURG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387454"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="VFY80_08120"
     CDS             <1..1923
                     /locus_tag="VFY80_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6007914.1"
                     /translation="FSGGGCVDKVGNYSFGTALFLLPHVIGTAGPVSGLVTDWTNVCA
                     HTRLFVAFGGLALKNGQVASGGPGAHDMEMWLRRARDAGIEFVVVSPNRGDAPDFLHA
                     HWISIRPGTDTAFLLGMAHELVSAGLEDRAFLARYCTGYEPFERYLTGRDDGQPKSPE
                     WAAPICGVDAAEIRALARKLAATRSLLTMTWSIQRAHHGEQPYWMLITLAAMLGQIGL
                     PGGGFGFGHGSQGGVGSPRPAGAATAELPVGKNPASTAVPSARLTDMLERPGEEYDFN
                     GERLRYPHIRLVYWAGGNPFHHHQDLNRLRQAWQRPDTVIVHETHWTPTARHADIVLP
                     ATTTLERNDLGGASRDRFLLAMHRAIDPIARARNDRDIFGELAARGGFEARYTEGLDE
                     TGWIRRLYDSTREKNLTAGIEMPPFEVFWERGYFELPRPQRAFVLFEDFRNDPLAHPL
                     KTPSGRIEIYSTRIAGFNYDDCPPHPTWLPPSEWLGASQTRVYPLHLVTTQPPDKLHS
                     QADYGPVARAAKRAGHERIRLSSADASMRSLTDGTVVRVFNARGACLATVEVDAGLRT
                     GVAIMSTGAWYDPADGNDRALERHGNPNVLSLDQGTSKLSQGPSALSMLVEVERWDGA
                     HATSAHTPPQIVASVG"
BASE COUNT          354 a          714 c          723 g          368 t
ORIGIN      
        1 ttcagcggcg gtggctgcgt cgacaaggtg ggcaattaca gcttcggcac cgccttgttc
       61 ctgctgccgc acgtgatcgg caccgcaggc ccggtgtcgg gcctcgtgac cgactggacg
      121 aacgtctgcg cgcatacgcg gctcttcgtc gcgttcggcg ggctcgcgct caagaacggg
      181 caggtggcct ccggaggtcc cggagcgcat gacatggaga tgtggctgcg ccgcgcgagg
      241 gatgcgggca tcgagttcgt cgtcgtgagc ccgaaccgcg gcgatgcgcc cgatttcctg
      301 cacgcgcact ggatctcgat ccggcctgga accgacacgg cgtttctgct gggcatggcg
      361 cacgagctgg tgagcgcggg gctcgaagac cgcgctttcc tcgcccgcta ttgcaccgga
      421 tacgagccgt tcgagcgcta tctcacgggg cgcgacgacg gccagccgaa gagcccggaa
      481 tgggcggcgc cgatctgcgg cgtcgatgcg gcggaaatcc gcgcgctcgc acgcaagctc
      541 gcggcgacgc gcagtctatt gacgatgacc tggtcgattc agcgtgcgca ccacggcgag
      601 cagccgtatt ggatgctgat cacgctcgcc gcgatgctcg gtcagatcgg gctcccgggc
      661 ggcggcttcg ggttcggtca cggctcgcaa ggcggcgtcg gcagtccccg gccggccggc
      721 gcggccacgg ccgagctgcc cgtcggcaag aatcccgcga gcacagcggt tccgtcggcg
      781 cgactgaccg atatgctcga gcgtccgggc gaagagtacg acttcaacgg cgagcggctg
      841 cgctatcccc atattcgtct ggtctattgg gccggcggca atccatttca tcatcatcag
      901 gatctgaatc ggctgcggca ggcctggcag cgcccggata cggtgatcgt ccacgaaacg
      961 cactggacgc cgaccgcgcg ccacgcggac atcgtgctgc cggcgacgac gacgctcgag
     1021 cgcaacgacc tgggcggcgc gtcgcgcgat cgattcctgc tcgcgatgca tcgcgcgatc
     1081 gatccgatcg ctcgagcacg caacgaccgc gatatcttcg gcgagcttgc cgcgcgtggc
     1141 ggcttcgagg ctcgctatac cgaagggctg gacgaaaccg gctggatccg caggctctac
     1201 gacagcacgc gcgaaaagaa tctgactgcc ggcatcgaga tgccgccgtt cgaggtcttc
     1261 tgggagcgcg gctacttcga gctgccgcgg ccgcagcgcg cgttcgtcct gttcgaggac
     1321 tttcgcaatg atccgctggc gcatccgctg aaaaccccga gcggcaggat cgagatctat
     1381 tcgacgcgga tcgccggctt caactatgac gactgtccgc cgcacccgac ctggctgccg
     1441 ccgagcgagt ggcttggggc ctcgcagacg cgcgtctacc ccttgcatct ggtcaccacg
     1501 caaccgcccg acaagctgca cagccaggcc gattacgggc cggtcgcgcg cgcggccaag
     1561 cgcgcgggtc acgagcgcat ccgcttgagc agcgcagacg cgagcatgcg ttcgcttacc
     1621 gacggcacgg tcgtgcgtgt tttcaatgcg cgcggcgctt gcctggcgac ggtcgaagtg
     1681 gatgcgggct tgcgcaccgg cgttgccatc atgtctaccg gggcctggta cgaccctgcc
     1741 gatggcaacg accgcgcgct cgaacgccat gggaacccga acgtgctctc gctggatcag
     1801 gggacctcga agctgagtca gggaccgagc gcgctttcga tgctggtcga agtggagcgc
     1861 tgggacggcg ctcatgccac atccgcgcat actccgccgc agatcgtcgc cagcgtgggt
     1921 tagcccggcg taacgtgtct accttgccgg cgggttgtgt agcccgtaca cccacctatc
     1981 gagcttttga tgaattgcct gacgctcgat tccctcaccc gcaaccctct cccaagggga
     2041 gagaggggag cgtccagcgc taaatgagtt gtcactcatt tagcgaatcc tcaataggac
     2101 gtgagctcga attcgactgg atcgccggca gcctgcgccc gcgtcgtgtc gtagaccgg
//
LOCUS       DASURG010000461         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1512034, whole genome shotgun sequence.
ACCESSION   DASURG010000461 DASURG010000000
VERSION     DASURG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512034"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="dprA"
                     /locus_tag="VFY80_08125"
     CDS             <1..971
                     /gene="dprA"
                     /locus_tag="VFY80_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX6007915.1"
                     /translation="ASVRDLVRVVPHKVASDIERGPSPESMACAAAWLEDEANAIVTL
                     GDVRYPRILLEIHDPPPLLYIKGRAELLNGPALAIVGSRHATPQGIALAESFATTLSE
                     AGLTIVSGLALGIDAAAHRGGLAGRAKSMAVVGTGLDVVYPARNRALAHQLAASGVLI
                     SEFPLGTGPLAGNFPRRNRIISGLARGCLVIEAAAQSGSLITARLANEQGREVFAVPG
                     SVHSPLAKGCHQLIKQGAKLVDSALDVLEELHMPLPAGAPERAVSELEPHDAALLEHL
                     GYDPCDIDTLVTRSGLPADIVTATITRLELAGRVAGIPGAKYQRVR"
     gene            1134..>3247
                     /locus_tag="VFY80_08130"
     CDS             1134..>3247
                     /locus_tag="VFY80_08130"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEX6007916.1"
                     /translation="MDKRLIIAEKPSVAADIARALGGFTRHKDYYESDRYVLSYAVGH
                     LLELSAPEEFDPKRGRWSFRHLPVIPPRFDLKPINNKSAAQLNVLLKLMRRKDIAGLI
                     NACDAGREGELIFRYLVQHAGVKKPIERLWLQSMTPAAIREGFEQLRSDRQMRPLADA
                     AKSRSEADWLVGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLAILVEREQRIREFKAR
                     DYWEVHAQFGAKAGEYAGRWFDPAFTKGEDAESKSERLWSAAQAEAIAAACRGKSGQV
                     EEESKPATQLSPLLFDLTSLQREANGRFGFSAKTTLSLAQALYERHKVLTYPRTDSRA
                     LPEDYPGTVKKTLDALKAPYSAFAADILKHGWVKANTRIFNDAKVSDHFAIIPTMQAP
                     KSLNEAEAKLYDIVVRRFLAVFYPAAEYLQTVRITQVAEHKFRTEGRVLQRPGWLTVY
                     GKAAQEENENLPRVAPGERVAVLEVNAVPNQTKPPPRYSEATLLSAMEGAGKLVEDEE
                     LRAAMEAKGLGTPATRAAIIEGLLSENYIERLGRELKPTWKAFRLLFALKHFGVDEIT
                     SPELTGDWEYKLKQMEQARLPRQTFMDHIEQAAKELVERIRGGNIPDEAFVTVPAPCP
                     KCAGVVQETYRKFQCQSCDFYIWPVLLGREWSPEEVAELITRRYIGPLNGFRSKQGRP
                     FSAGLRLTDDLRIEFDFGQPSGS"
BASE COUNT          629 a         1022 c         1020 g          576 t
ORIGIN      
        1 gtgcgagtgt ccgggatctc gtgcgcgtcg tgccccacaa agtcgcgagc gacatcgagc
       61 gcgggccgtc accggaatcc atggcgtgcg ctgcggcttg gcttgaggat gaagcgaatg
      121 cgatcgtcac gctcggcgat gtgcgctatc cgcggattct cctcgagata cacgatcctc
      181 cacccctgct ctatataaaa ggccgcgccg agctcctgaa cgggccggcc ctcgcgatcg
      241 taggcagtcg tcatgcgacc ccgcaaggaa tcgcgctcgc cgaatcgttt gcgacgacgc
      301 tgagcgaagc gggcttgacg atcgtcagcg gtcttgcgct cggaatcgat gccgcagctc
      361 accgcggcgg gcttgccggt cgcgcaaaaa gcatggccgt ggtaggtacc gggctcgacg
      421 tggtctatcc cgcgcgtaac cgggcgctcg cgcatcaact cgcggcgagc ggcgtgctca
      481 tttccgaatt tccattgggc accgggccgc tcgcgggaaa ctttcctcgt cgcaaccgca
      541 tcatcagcgg ccttgcgcgc ggatgcctcg tcatcgaagc ggcagcgcaa agcggctcgc
      601 tcattacggc gcgacttgca aacgagcagg gcagagaagt tttcgcggtg cctggctcgg
      661 tgcattcgcc gctcgcgaag ggatgccatc aactcatcaa acagggtgcc aagctcgtcg
      721 atagcgcgct cgacgtgctg gaggagctcc acatgccgct ccccgcgggc gcgccagaaa
      781 gggccgtctc cgagctcgag ccacacgatg cggcgcttct cgaacatctc ggatacgatc
      841 cctgcgatat cgacacgctg gttacgcgca gcggtctccc cgcggacatc gtcaccgcca
      901 caatcacgcg cctcgaactc gcggggcgcg ttgccggcat cccgggcgcc aagtaccagc
      961 gcgtgcgctg atcgccattt gcgcgcaagt cgttcgcgaa tagcgctttt tcgcgagccg
     1021 cagatctcgc gcttgcgctt gaataggttt gttgtatcat cgtccgctct ttttcgctcg
     1081 cgtcacgaca tgcgacgtgc tcgcagcgtt cttatctatc tcagccgcgc gccatggata
     1141 aacgactgat catcgcggag aagccttcgg tggccgcgga catcgcgcgc gcgcttggtg
     1201 gcttcacgcg gcacaaggat tactatgaga gcgatcgcta cgtgctctcg tacgcagtcg
     1261 ggcatctgct cgaactgtcc gcgccggagg agttcgatcc caagcgcggc cggtggtcgt
     1321 ttcgccacct gccggtcatt ccgccgcgat tcgatctcaa accgatcaac aacaagagcg
     1381 ccgcgcaact caacgtgctg ctcaagctca tgcgcagaaa ggatatcgca ggactcatca
     1441 acgcctgcga cgcggggcgc gaaggcgagc tcatctttcg ctacctcgtg cagcacgcgg
     1501 gcgtcaaaaa accgatcgag cgactgtggc tgcagtccat gacgcctgcc gccatacgcg
     1561 agggcttcga gcaactgcgc agcgatcgtc agatgcggcc tctcgcggat gccgcaaagt
     1621 cgcgatccga ggccgactgg ctcgtcggca tcaacggcac ccgggcgatg acggcattca
     1681 attcaaagga gggcggcttc tatctcacca ctgtgggccg cgtccagacg ccgacgctcg
     1741 caatcctggt cgagcgggaa cagcgcattc gggaattcaa ggcacgcgac tactgggaag
     1801 tgcacgcgca attcggtgcg aaagccgggg aatatgcggg gcgctggttt gatcccgcct
     1861 tcacgaaggg cgaggatgcc gagtcgaaga gtgaacggct gtggagtgcg gcccaagccg
     1921 aagcgatcgc cgctgcgtgt cggggcaaga gcggacaagt cgaggaggag agcaagccgg
     1981 cgacccagct ctctccgctg ctcttcgacc taaccagcct tcagcgcgaa gcgaatggcc
     2041 gctttggctt ttcggcgaaa acgactcttt cgctcgccca ggccttatac gagcggcaca
     2101 aggtgcttac gtatccaagg acggactcgc gcgcgctgcc cgaagattac ccgggtacgg
     2161 ttaagaagac gctagacgcc ctcaaggcgc catactcggc cttcgcagcc gatatcctga
     2221 agcatggctg ggtcaaggcg aacacgcgta tcttcaatga tgcgaaggtg tcagatcact
     2281 tcgccatcat tcccaccatg caggcgccga agtcactgaa tgaggccgaa gcgaagctct
     2341 acgatatcgt ggtccgccgc ttcctggcgg tgttctatcc cgcggcggaa tacctgcaga
     2401 ccgtgcgcat cacccaggtt gcggagcata agtttcgtac cgagggccgc gtactgcagc
     2461 gccccggctg gctcaccgtc tacggcaagg cggcgcagga agaaaacgag aatctcccgc
     2521 gcgtggcgcc gggggagcgc gtcgcggtat tggaggtgaa cgccgtccca aaccagacca
     2581 agccgcctcc tcgctattcg gaggccacac tgctgtccgc aatggaaggc gccggcaagc
     2641 tggtggagga cgaagagctg cgcgccgcca tggaggccaa ggggctgggt acgccggcca
     2701 cgcgtgccgc catcatcgag gggctgctca gcgagaacta cattgagcgg ctcggccgcg
     2761 aactgaagcc tacgtggaaa gcattccgcc tcctcttcgc gctcaagcat ttcggcgtgg
     2821 acgagatcac ctcgccggag ctcaccgggg actgggaata caagctgaaa caaatggaac
     2881 aggcacgcct gccgcgccaa accttcatgg accacatcga gcaggccgca aaggagctgg
     2941 tggaacgcat ccgtgggggc aacattccgg atgaggcctt tgtgaccgtg ccggcgccgt
     3001 gccccaaatg cgcgggcgta gttcaggaga cctatcgcaa gtttcagtgc cagtcttgcg
     3061 acttctatat ctggccggtg cttctcggcc gcgaatggtc gccggaagaa gtggcggaat
     3121 tgatcacgcg ccgctatatc gggccgctca acggatttcg cagcaagcag gggaggccgt
     3181 tctctgcggg acttcggctg accgatgact tgcggatcga gttcgatttc gggcaaccga
     3241 gcggatc
//
LOCUS       DASURG010000462         7676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1101926, whole genome shotgun sequence.
ACCESSION   DASURG010000462 DASURG010000000
VERSION     DASURG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101926"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFY80_08135"
     CDS             complement(<1..497)
                     /locus_tag="VFY80_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6007917.1"
                     /translation="MDIEYWWLLALPLFFALGWLAARIDIKQLVSESRALPVSYFKGL
                     NFLLNEQPDKAIEAFIEVVKVDPQTIELHFALGSLFRRRGEVERAIRMHQNLVERSDL
                     QEEQRLTALFELAQDYLKAGLLDRAEELFLKLDNTVHAEAALKSLLEIYEQEKDWQKA
                     IHVAG"
     gene            complement(519..812)
                     /locus_tag="VFY80_08140"
     CDS             complement(519..812)
                     /locus_tag="VFY80_08140"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX6007918.1"
                     /translation="MRYFLWILKFALFVVVLSFAVKNTETVTVRYYLGLEWQAPLVLV
                     LLTFFCLGVAVGIMASLGRLFRQRRQISVLKGELRAQVEDGTRSSADGVSGTP"
     gene            complement(931..1218)
                     /locus_tag="VFY80_08145"
     CDS             complement(931..1218)
                     /locus_tag="VFY80_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX6007919.1"
                     /translation="MTKSELIARLAARYPQLVAKDAELAVKMILDAMAKSLAQGQRIE
                     IRGFGSFGLNYRPPRTGRNPKSGEKVQVPAKYVPHFKAGKELRERVDFKKQ"
     gene            complement(1228..2943)
                     /gene="rpsA"
                     /locus_tag="VFY80_08150"
     CDS             complement(1228..2943)
                     /gene="rpsA"
                     /locus_tag="VFY80_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6007920.1"
                     /translation="MKTSTPVSGSAENFAALFEESLSRKEMRIGEVITAEIVRVDENF
                     VVVNAGLKSESYISAEEFRNDRGELDVKPGDFISVAIESLENGYGETRLSRDKAKRLA
                     AWHELEEALEKGTMVTGVINGKVKGGLTVTVNGIRAFLPGSLVDIRPVKDTTPYENRQ
                     MEFKVIKLDRKRNNVVVSRRAVLEETQGEERQKLLESLQEGAVVKGIVKNITDYGAFV
                     DLGGIDGLLHITDLAWRRVKHPSEILSVGDEVEAKVLKFDQEKNRVSLGLKQLGEDPW
                     VGLSRRYPPGTRLFGKVSNLTDYGAFVEIEPGIEGLVHVSEMDWTNKNVHPSKVVQLG
                     DEVEVMVLEIDEERRRISLGMKQCMPNPWEEFAMNYRKGDRVKGQIKSITDFGIFIGL
                     PGGIDGLVHLSDLSWSQPGEEAVRNYRKGEEVEAMVLSIDVERERISLGIKQIEGDPF
                     TSYIAAHEKNSVVTGTVKSIDAKGAVIQLDGDVEGYLRSSEVARDRVEDIRTRLKEGD
                     SVTAMIINIDRKNRTINLSIKAKDMAEEAEAMQKLASEKQASAGTTNLGALLKAKLDT
                     AKTEQ"
     gene            complement(3017..3688)
                     /gene="cmk"
                     /locus_tag="VFY80_08155"
     CDS             complement(3017..3688)
                     /gene="cmk"
                     /locus_tag="VFY80_08155"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX6007921.1"
                     /translation="MPQVVPVIAIDGPSASGKGTVALGVAQALGFHFLDSGALYRLVA
                     YAALAQGASLESESQVANIARNMKATFRSDGVTLDGRDVSLEIRTEAVSAAASRVAAL
                     PAVRAALIERQRGFRRPPGLVADGRDMGSVVFPDATLKIFLTASAEERARRRYKQLME
                     KGLSANMALLLQDIRERDARDSARAVAPLQICADAIEIDTTAMTVAEAVSRILALYAQ
                     AARAP"
     gene            complement(3692..5014)
                     /gene="aroA"
                     /locus_tag="VFY80_08160"
     CDS             complement(3692..5014)
                     /gene="aroA"
                     /locus_tag="VFY80_08160"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX6007922.1"
                     /translation="MDHLDLAPIRRIRGTVRLPGSKSISNRILLLAALARGDTRIHDL
                     LESDDTGHMLEALRALGVECRPDNKRSLTVRGAAGVPPVKEARLFLGNAGTAFRPLAA
                     VLALAHGHYTLSGVARMHERPIGDLVDALTQLGADVRYLGKRGYPPLEVHPGQLRPRD
                     AVSVRADVSSQFVTALLIALPLLGKPIRVHIVGELTSKPYVEITLNTLRRFGVAVARE
                     GWSAFTVPQTSGYASPGEIFVEGDASSASYFLAAGAISGLTGGGPVRVEGVGRDSIQG
                     DVRFTEILERMGAEITFGDNWIEAQASVSAKNSGRLSAIDADLNHIPDAAMTVAMLAL
                     FADAPSTLRNIGSWRVKETDRIAAMATELRKLGALVEEGADYLRVTPPPGRALNAGAE
                     IDTYDDHRMAMCFSLVALGGVPVRIRDPGCVAKTFPQYFDVLQSISER"
     gene            complement(5030..5908)
                     /locus_tag="VFY80_08165"
     CDS             complement(5030..5908)
                     /locus_tag="VFY80_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6007923.1"
                     /translation="MPAKRKINKLVVIGVGLIGGSFALALKRARAVSRIIGIGRTKRN
                     LALARRRGIIDEIALDAGQAVRDADFVFLGTPVGQMPDVMARIAPHLSSRVVLTDGGS
                     TKQDVIEYARMYLGDHYSRFVPSHPIAGTELSGAGAAFADLFRNRQVIVTPTPETSAS
                     ALRAVRAAWHRCGARLIRMEAQRHDDILAAVSHLPHVIAFTLMNALARRPDALAHSGG
                     GLRDTVRIAGSSPEMWRDICLANREALLRALDGYTSELARCRAAIERGDGAALESMFG
                     EARAARSRWLLRRRTQ"
     gene            complement(5919..7019)
                     /gene="hisC"
                     /locus_tag="VFY80_08170"
     CDS             complement(5919..7019)
                     /gene="hisC"
                     /locus_tag="VFY80_08170"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6007924.1"
                     /translation="MSLCDLAPPYIRSIAPYQPGKPISELAREMGLDEGSIIKLASNE
                     NPLGVSPRARRAIERELDELSRYPDGNGFALKQALCARYGVAREQIVLGNGSNDVLDL
                     AARAFLTPGLSAIYSQHAFAVYPLAVQAIGAVGIEVPAADFGHDPDAMLRTVRPDTRL
                     IFIANPNNPTGTMIPGPRLEAFIAAVPKSVLVVLDEAYSEYVPPEAAYDSFAWLRRYP
                     NLVITRTFSKVYGLAGLRVGCAFAAEEVADLMNRVREPFNVNSVALAAAAAALEDTDF
                     VQRSVAANASGMRQITAGLKRLRLEQIPSYGNFVTFRVRDAASVYQRLLKQGVIVRPI
                     ASYGMPQHLRVTIGLESENERFLESLERSLAG"
     gene            complement(7041..>7676)
                     /locus_tag="VFY80_08175"
     CDS             complement(7041..>7676)
                     /locus_tag="VFY80_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX6007925.1"
                     /translation="VGRTLDLLLTTPARVCGEIMLPVRQCLMAKDKRRDKIRRVYSHT
                     QSLAQCQRWIARHLSHTETVAVVSNAEAARRASAEKNAAAIAARTAAALYGLHLLARN
                     IEDEPRNTTRFLVLAERDAPPAGRDKTSLILSTRNVPGAVHELLAPLAENQVSMTRLE
                     SRPARTELWEYVFYVDIEGHQQDENVKRALAELERRASFLKNLGSYPAAAQ"
BASE COUNT         1361 a         2485 c         2381 g         1449 t
ORIGIN      
        1 ttgccggcta catgaatcgc cttctgccag tccttctcct gctcgtagat ttcgagcagc
       61 gacttgagcg ccgcctcagc gtgcacggta ttgtcgagct tgagaaaaag ctcttcggcg
      121 cggtcgagca gccccgcctt caagtaatcc tgagcaagct cgaagagcgc ggtgagccgc
      181 tgctcttcct ggagatcgga gcgctcgacg aggttctgat gcatgcggat cgcgcgttcc
      241 acttcgccgc gccggcggaa gaggctcccg agcgcgaagt gcagctcgat cgtctgcgga
      301 tcgactttca ccacctcgat gaaggcctcg atcgccttgt ccggctgctc gttgagcagg
      361 aagttgaggc ccttgaagta ggataccggc agcgcccgcg actcggaaac gagctgcttg
      421 atgtcgatgc gggccgcgag ccaccccagc gcgaaaaaga gcggcagcgc aagcagccac
      481 cagtattcaa tatccatgat gcgaggccgc gatatcgctc acggcgtgcc cgaaacgccg
      541 tccgccgacg accgcgtgcc gtcctcaacc tgcgcgcgca attcgccttt caacacggag
      601 atctggcgcc gctggcggaa gagacgccca aggcttgcca tgatgcccac tgccacaccc
      661 agacagaaaa aggtgagcag caccagcacg agcggcgcct gccattccag acccaggtag
      721 tagcgcacgg tgacggtctc ggtgttcttc accgcgaagc tcaggaccac gacgaacagc
      781 gcaaacttga gaatccagag gaagtagcgc ataagcggag cttaattgaa gctgcggcgt
      841 ctgtcagcgc gccggcgcgc cccggtggcg ggcaaaaaaa gacggcacga tctttcgatg
      901 atgccgtcct ggacagacga tgccgtcgtc ttactgtttc ttgaaatcca cgcgctcgcg
      961 caattccttc cccgccttga aatgcggtac gtatttcgcc ggcacctgca ccttctcgcc
     1021 ggacttggga ttgcgtcccg tgcgcggggg ccgatagttc aagccgaaac tgccgaatcc
     1081 cctgatctcg atgcgctgcc cctgcgcaag gcttttcgcc atcgcgtcca ggatcatctt
     1141 gaccgcgagc tcggcatcct tcgccaccaa ttgcggatag cgtgccgcaa gtcttgcgat
     1201 cagctccgat ttggtcatgg cgcttcctta ttgttcggtc ttcgccgtat cgagcttggc
     1261 cttgagcagt gcaccgaggt tggtggtgcc ggcacttgcc tgtttttcgg aagcgagctt
     1321 ctgcatcgcc tccgcctcct cggccatgtc cttcgccttg atcgaaagat tgatggtgcg
     1381 gttcttgcga tcgatattga tgatcatcgc cgtgaccgaa tcgccttcct tgagccgcgt
     1441 gcgaatgtcc tccacgcggt cgcgagcgac ctcggacgag cgcagatatc cttccacgtc
     1501 gccgtcgagc tggatcaccg cgcccttggc gtcgatcgac ttcaccgtgc cggtgacaac
     1561 gctgttcttt tcgtgcgccg cgatgtagct cgtgaaggga tcgccttcga tctgcttgat
     1621 gccgagcgaa atgcgctcgc gctcgacgtc gatcgagagc accatcgctt ccacttcctc
     1681 gcccttgcgg tagttgcgta ccgcctcttc gccgggctgc gaccaggaaa ggtcggagag
     1741 gtgcacgagg ccgtcgatgc cgccggggag cccgataaag atgccgaagt cggtgatcga
     1801 cttgatctgg cccttcacgc gatcgccctt gcgatagttc atcgcgaatt cttcccacgg
     1861 attcggcatg cactgcttca tgccgagcga gatgcgccgg cgctcttcgt cgatctccag
     1921 caccatgact tcgacttcat cgcccagctg cacgactttc gagggatgga cgttcttgtt
     1981 ggtccagtcc atttcggaga cgtgcacgag cccctcgatg ccgggctcga tctcgacgaa
     2041 tgcgccgtag tcggtcaagt tgctcacttt gccgaagagg cgcgttccgg gcgggtagcg
     2101 gcgtgagaga ccgacccacg gatcttcgcc gagctgcttc aagccgagcg acacgcggtt
     2161 cttctcctga tcgaacttga gcaccttcgc ttccacttcg tcgccgaccg agaggatctc
     2221 agaggggtgc ttcacgcgcc gccacgcgag gtcggtgatg tgcagcagcc catcgatgcc
     2281 gccgagatcg acgaacgcgc cgtagtcggt gatgttcttg acgatgccct tcaccaccgc
     2341 gccttcctgc aggctctcga gcagcttctg gcgctcctcg ccctgcgttt cctcgagcac
     2401 ggcgcggcgc gagacgacca cattgttgcg cttgcggtcg agcttgatga ccttgaactc
     2461 catctgcctg ttttcgtacg gcgtggtgtc cttcaccggc cggatgtcga cgagcgagcc
     2521 ggggaggaag gcgcgaatgc cgttcaccgt gacggtgagc ccgcccttca ctttgccgtt
     2581 gatgacgccg gtcaccatgg tgcccttctc gagagcttcc tcgagctcgt gccacgccgc
     2641 gagccgcttc gccttgtcgc gcgaaagccg ggtctcgcca taaccgtttt ccagggactc
     2701 gatcgcgacg ctgatgaaat cacccggctt cacgtcgagc tctccccggt cgttccggaa
     2761 ctcttcggcg gagatgtagc tttctgattt caagcccgcg ttgaccacca cgaaattctc
     2821 gtcgacgcgc acaatttcgg cggtgatcac ctcgccgatg cgcatctcct tgcgggagag
     2881 gctctcttca aacagcgcgg cgaaattttc cgcggaccca gaaaccgggg tagaagtttt
     2941 catcattcag attcagtgtg ttaggaaacc cgtccgctcg acggggtggg tttaaaaaac
     3001 cacgagtcga gccgactcat ggtgcccgtg ctgcctgcgc gtacagcgcc agtatccgag
     3061 ataccgcctc cgccaccgtc atcgcggtcg tatcgatctc gatggcatcg gcgcatattt
     3121 gcaggggggc cacagcgcgc gcactgtcgc gtgcgtcgcg ttcccggatg tcctgcaaga
     3181 ggagcgccat gttagcactc aacccttttt ccatcaactg cttatatcgc cggcgcgcgc
     3241 gttcttcggc gctcgcggtg aggaagatct tgagcgtcgc atcgggaaaa acaaccgatc
     3301 ccatgtcccg cccatcggcg acgagccccg gcggccggcg gaacccgcgt tggcgctcga
     3361 tcagggcagc gcgcactgcg ggcagagccg ctacacgaga cgccgcagca ctcaccgctt
     3421 ccgtgcggat ctcgaggctc acgtcgcgcc cgtcgagtgt gacgccgtcg ctccgaaaag
     3481 ttgctttcat attccgcgcg atattggcaa cttgtgattc actttctagg ctcgcgccct
     3541 gcgcaagggc cgcatacgca accaagcgat acagggcgcc gctgtcgaga aagtgaaacc
     3601 ccagcgcctg cgccaccccc agcgccacag tccccttccc cgaagcagag ggaccgtcta
     3661 tcgcgatgac ggggacgacc tgcggcacgg ttcagcgctc ggaaatgctc tgcaggacgt
     3721 cgaagtactg cggaaaagtc ttggcaacgc agccggggtc tcgaatccgc accggaacgc
     3781 cgccgagcgc cacaagcgaa aagcacatcg ccatccggtg gtcgtcgtag gtatcgattt
     3841 cggcacccgc attcagcgcc cggccagggg gtggcgtgac gcggaggtaa tcggcgccct
     3901 cctccacgag cgcaccgagc ttgcgcagtt cggtcgccat ggcggcgatg cgatcggttt
     3961 ccttgacgcg ccagctaccg atgttgcgca gcgtcgatgg cgcatccgca aagagcgcca
     4021 gcatcgcaac cgtcatcgcc gcatccggaa tgtgattgag atctgcgtcg atggcgctca
     4081 gcctgccgct gttctttgcc gacacgctcg cctgcgcttc gatccagttg tcaccgaatg
     4141 tgatctcggc gcccattcgc tcgagaattt cagtaaaccg cacgtcgccc tggatcgaat
     4201 cgcggcccac gccttccacg cgcaccggcc caccaccggt gagcccgctg atcgcacccg
     4261 ccgcaagaaa atacgacgcg gaggatgcat cgccttccac gaagatctct cccgggctcg
     4321 catatccgga tgtctgagga acggtgaacg cggaccatcc ttcgcgcgcg accgccacgc
     4381 cgaaacggcg cagggtattg agcgtgatct cgacgtacgg cttcgacgtg agctcgccca
     4441 cgatgtgcac gcggatcggt tttccgagca gcggaagcgc gatcaggagt gcggtcacga
     4501 actggctcga gacatccgca cgcacactga ctgcgtcccg cggtctcagc tggccgggat
     4561 gcacctcgag tggaggataa ccgcgcttgc ccaaatagcg aacgtcagcc cccagttgcg
     4621 tcagtgcatc gacgaggtcg ccaatgggcc gctcgtgcat gcgcgcgaca ccggagagcg
     4681 tgtagtgccc atgagccagg gccagcacgg cagccagcgg acgaaaggcg gtgccggcgt
     4741 tgccgaggaa aagtcgcgct tccttcaccg gtggcacgcc cgcggcaccc cgcaccgtga
     4801 ggctgcgctt gttgtcggga cggcactcca cgccgagcgc acgcagcgcc tcgagcatgt
     4861 ggccggtgtc gtccgattcg agaaggtcgt gaatgcgggt gtcgccgcgc gcgagcgcag
     4921 caagcagcag gatgcggttc gagatgctct tcgagcccgg cagccttacc gtgccgcgaa
     4981 tccgtcggat cggcgcgaga tcgagatgat ccatcccggc gatgttgcgc tactgcgtgc
     5041 gtcgccgcaa cagccagcgc gaccgggctg cgcgcgcctc accgaacatc gattcgagcg
     5101 cagcgccatc tccgcgctcg atggcggcgc ggcagcgtgc gagctccgac gtatagccgt
     5161 cgagcgcgcg aaggagcgct tcccggtttg cgagacagat gtcgcgccac atttccggcg
     5221 agctgcccgc gatgcgtacc gtatcgcgca gcccgccgcc cgaatgcgcg agcgcatcgg
     5281 ggcgccgggc gagcgcattc atgagcgtga acgcgatgac atgcggcagg tggctgacgg
     5341 ctgcgagaat gtcgtcgtgc cgttgtgcct ccatgcggat caaacgtgcg ccgcatcgat
     5401 gccacgccgc gcgaaccgcg cgcaatgcgc tcgcgcttgt ttccggcgtg ggcgtcacga
     5461 tcacctggcg attgcggaac aggtccgcaa acgccgcccc tgcgccgctc aattccgttc
     5521 ctgcgatggg atgcgaaggc acgaagcgcg aatagtgatc gccgagatac atgcgtgcat
     5581 actcgatgac gtcctgcttg gtgctgccgc catcggtgag caccacccga gaggaaagat
     5641 gcggtgcgat acgggccatc acgtccggca tctgtcccac gggtgtcccg agaaaaacga
     5701 agtcggcatc gcgcaccgcc tgcccggcgt cgagagcgat ctcatcgatg atgccgcgcc
     5761 ggcgggcgag cgcgagattc cgcttcgtgc gtccgatgcc gatgatgcgg ctcactgccc
     5821 gcgctcgctt gagcgcgagc gcgaacgatc cgccgatcag tccgacgccg atgacgacga
     5881 gcttgttgat ttttcttttt gccggcatgc ggcgctgttc agcctgcgag cgagcgctcg
     5941 aggctctcga ggaagcgctc gttttccgac tcgagaccga tggtgacccg caggtgctgc
     6001 ggcatgccgt agctcgcaat gggccgcacg atgacaccct gcttgagtag cctttggtat
     6061 acgctcgccg catcgcgcac ccggaaggtc acgaaattgc cgtaagacgg aatctgctcc
     6121 agtcgaagcc gcttcaggcc agcggtgatc tgcctcatgc cggacgcgtt agccgcaacg
     6181 ctccgctgca cgaaatcggt gtcttccagc gcagccgccg cggctgcgag tgcgacgctg
     6241 ttcacgttga acggctcgcg cacgcggttc atgagatcgg caacctcctc tgcggcaaaa
     6301 gcacacccta cgcgcagtcc ggcgagcccg tacaccttgg agaaggtccg cgtgatgacg
     6361 aggttgggat agcgcctgag ccacgcgaag ctgtcatacg cggcttccgg cggcacgtac
     6421 tcgctatagg cctcgtcgag aaccacgagc acgctcttcg gcacggcggc aataaacgct
     6481 tccaggcgcg ggccgggaat cattgtgccg gtcggattat tgggatttgc gatgaagatg
     6541 agccgcgtgt cgggccgcac tgtgcgcaac atcgcatcgg ggtcgtgccc gaagtccgca
     6601 gcaggcactt cgatccccac ggcgccgatc gcctggacgg cgagcggata gaccgcaaag
     6661 gcatgctgcg aataaatggc gctcagcccc ggtgtcagga acgcgcgcgc cgcgagatcc
     6721 aatacatcgt tcgagccatt gccgagaacg atctgctcgc gcgcgacacc gtaacgcgcg
     6781 cagagcgcct gcttcaacgc gaagccgttg ccgtccggat agcgcgacag ttcgtcgagt
     6841 tcccgctcga tggcccgacg cgcgcgcggg ctcaccccga gcggattctc gttcgaggcg
     6901 agcttgatga tggacccttc atccaggccc atctcgcgcg cgagttcgga gatcggcttt
     6961 ccgggctgat acggcgcgat gctgcggatg tagggcggtg caagatcgca gagactcatg
     7021 ctccaattga ccgggttggg ttattgggcg gctgcgggat acgagccgag attcttgaga
     7081 aaagaggcac gccgctccag ctcggcgagc gcgcgcttca cgttctcgtc ctgctggtgg
     7141 ccctcgatat cgacgtagaa gacatattcc cagagctcgg tccgcgcggg ccgcgattcg
     7201 agccgtgtca tgctcacctg gttctccgca agcggcgcga gaagctcgtg aacggcaccc
     7261 gggacattgc gtgtggagag aatgagtgaa gtcttgtcgc gccccgccgg cggcgcgtcg
     7321 cgttcggcaa gcacgagaaa gcgcgtcgta ttgcgcggct cgtcttcgat attgcgtgcg
     7381 agcaggtgca gtccgtagag tgctgccgcg gtgcgcgctg caatcgcggc ggcattcttc
     7441 tcggcgctcg cgcggcgtgc ggcctcggca ttgctcacga cagctaccgt ttccgtgtgt
     7501 gacaggtgcc gcgcgatcca gcgctggcac tgcgccaggc tttgcgtgtg cgaatagacg
     7561 cggcggattt tgtcgcggcg cttgtccttc gccataagac attgccgcac cgggagcatg
     7621 atttctccgc acactctggc gggcgtggtg agaagcaggt cgagcgtccg cccgac
//
LOCUS       DASURG010000463         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1512234, whole genome shotgun sequence.
ACCESSION   DASURG010000463 DASURG010000000
VERSION     DASURG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512234"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFY80_08180"
     CDS             complement(<1..944)
                     /locus_tag="VFY80_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007926.1"
                     /translation="MALPLFAGVQAGVHCRMVTRICAPALLLLFLASTAPAQDADAVA
                     RKLESYRSNTPFLYALLREMPKGGDLHSHLSGAVYAETFIAWAVEDSMCLSMTTLGTA
                     NCNEGGVIRADSLLRDQTLYDRVIDAWSTRNWRQEMRSGHYDFFETFGKFGGVGGSRD
                     GDMLAEVMRRAHAGRVSYLELMNTPNDGAGALGARVGWSADYGQLRARLRAAGHAEVV
                     AAARRDVAGWIARQRAIMQCDSRTPEPACAVTVRFLYQISRGRAPERVFAQLMTAFEV
                     AAADSMVVGFNMVQPEDGYLSMRDYSLQMGMIDYLHQF"
     gene            complement(967..1575)
                     /locus_tag="VFY80_08185"
     CDS             complement(967..1575)
                     /locus_tag="VFY80_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007927.1"
                     /translation="MRRFLTAALVSWLVLASPACDSPTVPPPDAQYNFMNGPETLPQV
                     LRFGERYALGIIDPNTGLRVIAGLPANPASHIGCQVFGPPFNGTEDFQVQSHQVIGTE
                     PPFVQILNTVDVNLHVYQNSTFNGFCRSTVYAQGTGKMVGTDNDLTASGERWNIWGWH
                     MSGDVIVTATGETARLNAFNRFRANSSSTEPVELLIRRVELN"
     gene            1857..>3316
                     /locus_tag="VFY80_08190"
     CDS             1857..>3316
                     /locus_tag="VFY80_08190"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX6007928.1"
                     /translation="MLLATITALTIATVGSFQLVQTQSAPAPPRTAATTAVAQRAATP
                     PIIDGVQSDEVWRSAPRQSDFFEFQPKIGTPPRFNTEFQVSYDDRNLYVFVRAYDPHP
                     DSIRHALTRRDVRGPSDQIKIIVDSYNDNRTGFQFAVNPDGVKRDYAVYDDDNEDDSW
                     NGVWDVATQQDSLGWTAEFQIPLSQLRYASAGTHEFGFGVWRDIERYSERVSWPEYKP
                     NTNGFISQMGELTGLAGLTSARRLELTPYAVTKNVSRQSEPGGFDRAQEITVGGDLKY
                     GITPNITLDATINPDFGQVEADPAVLNLSAFETFLRERRPFFVEGTGVYSSFRLNCYI
                     VVDCNTNEGLFYSRRIGRAPTLLSDYGDATTPASTPIAGAAKITGRISSGLSFGLLEA
                     TTLRVEGTDDRTVEPFSNFAVFSAQQDLRGGNTGINLIATAVNRSLDEWTEDVLHSSA
                     YAAGASFRNRFANRRYELNASFAVSRVAGSAAAIRRT"
BASE COUNT          654 a         1040 c         1035 g          587 t
ORIGIN      
        1 tagaactggt gcaggtaatc gatcatgccc atctgcagcg aatagtcgcg catcgatagg
       61 taaccatctt ccggctgcac catgttgaag ccgacaacca tggaatcggc ggccgccact
      121 tcaaaggcgg tcatgagctg cgcgaacacg cgctcgggcg ctcgcccgcg gctgatctgg
      181 tagagaaaac gcactgtgac cgcacaggcc ggctccggtg tccggctgtc gcattgcatg
      241 atggcgcgct gacgggcaat ccagccggcg acgtcgcgcc gcgcggccgc caccacctca
      301 gcatgtccgg ccgcgcgcag ccgcgcccgc agttgcccgt aatctgccga ccagcccacc
      361 cgtgcgccga gggcaccggc accgtcattc ggcgtgttca tcaactcgag gtaggacacc
      421 cgcccggcat gcgcgcgtcg catcacttcg gccagcatgt cgccatcgcg agatccaccc
      481 acgccaccga atttgccgaa ggtctcgaag aaatcgtaat ggccgctgcg catttcctgc
      541 cgccagttgc gcgtggacca ggcatcgatt acgcggtcgt agagcgtctg atcacgcagc
      601 aggctgtcgg cgcggatgac gccaccctcg ttgcagttgg ctgttcccag ggtggtcatg
      661 ctgagacaca tgctgtcctc gaccgcccag gcgatgaacg tctccgcata caccgcccca
      721 gacaggtgcg aatgcagatc gccgcccttg ggcatctcgc gcagcagcgc gtaaaggaac
      781 ggcgtgttcg accggtagct ctcgagtttg cgcgccacgg catcggcatc ctgcgcgggg
      841 gccgtggaag cgaggaacaa taggaggagg gcgggagcgc agatcctggt aaccatgcgg
      901 cagtgtacgc cggcctgcac ccccgcgaaa aggggcagtg ccatgcaccg cccctcacac
      961 cgtcgctcag ttcagttcaa cacgccgaat cagcaactcg accggctcgg tgctgctgga
     1021 gttggcgcgg aaccggttaa aggcattcaa gcgggccgtc tccccggttg cggtcactat
     1081 cacatcacct gacatgtgcc atccccagat attccagcgc tcaccactcg ccgtcaggtc
     1141 gttatccgtg cccaccatct tgcccgtacc ctgggcataa acggtcgagc ggcaaaagcc
     1201 gttgaatgtg ctgttctgat aaacgtgcag atttacatca accgtgttga gaatttgcac
     1261 gaagggcggc tccgtgccga tcacttggtg actctgcacc tggaaatcct cggtgccatt
     1321 gaagggcggg ccgaaaacct ggcaaccaat gtgggacgcg ggattcgcgg gtaacccggc
     1381 gatgacgcgc agtccggtgt tcggatcgat gatgccgagc gcgtaacgtt ccccgaaacg
     1441 caaaacctgc ggcagcgttt ccggaccgtt catgaaattg tactgcgcat cggggggcgg
     1501 gacggtcggc gaatcacaag cagggctagc gagcaccaac cacgagacca gtgctgcagt
     1561 gagaaagcgg cgcattctgc acctccaggg agagagtggg gagaacagca cgttgcgcgg
     1621 cggagggtcg gccggcgggc gccgggatcg cgccgcgcgc gccagaaggt ctggcgggct
     1681 acaatacagg ttgaagcggc ggcggaagtc aagtcaggaa aatatcccta caaggcggcg
     1741 gttcgtcccg ggcgggttgc taaaaaacca gcatgtgcta gaaatttagc agtcgggcgc
     1801 gcaacccttt tcaggggtcg gcctgtccaa cccttaaact cttcaggacg cggaccatgc
     1861 tgctcgccac gattaccgcg ctgaccatcg caaccgtcgg gtcatttcag ctcgtgcaga
     1921 cgcaatccgc gccggcgccg ccacggaccg cggccacgac ggcagtggcg caacgcgcgg
     1981 ccacgccgcc gatcatcgat ggagtgcaga gtgatgaagt ctggcggagc gcaccccggc
     2041 aatccgactt tttcgagttc cagcccaaga tcggcacgcc gccgagattc aacaccgaat
     2101 tccaggtttc ctacgacgat cgcaatctgt acgtattcgt gcgcgcctac gacccgcatc
     2161 ccgacagcat ccggcacgcg ctcacgcgcc gcgacgtgcg cggtccctcc gatcaaatca
     2221 agatcattgt cgattcctac aacgacaatc gcaccggttt tcagttcgcg gtcaatccgg
     2281 atggggtgaa gcgcgactac gccgtatacg atgacgataa tgaagacgat tcctggaatg
     2341 gtgtgtggga cgtggccacg cagcaggatt cgctcgggtg gacggcggaa ttccagattc
     2401 cgctttcgca gctgcgttac gccagtgcgg gcacgcatga attcggattt ggtgtgtggc
     2461 gcgacatcga gcgctacagc gagcgggttt cctggccgga atacaagccg aataccaatg
     2521 ggttcatatc gcagatggga gagctgaccg ggttggccgg acttacttcg gcgcgccggc
     2581 tggagctgac gccctatgcg gtgaccaaga acgtatcgcg gcagagcgag ccgggcggat
     2641 tcgatcgcgc gcaggagatc acggtgggcg gcgacttgaa gtacggcatt acgcccaaca
     2701 tcacgctgga tgcaacgatc aatcccgatt tcggtcaggt cgaggccgat cctgccgtgc
     2761 tcaacctgag cgcgttcgag acctttctgc gggagcgccg gccgtttttc gtcgaaggca
     2821 ccggcgttta cagctctttc cgtctgaact gctacatcgt cgtggattgt aataccaacg
     2881 aagggctctt ctattcgcgc cgcatcggcc gggcacccac actgctgtcg gattatggtg
     2941 acgcgacgac gccggcctcg accccgatcg cgggggcggc caagatcacc ggccgcatca
     3001 gctcggggtt gtcattcgga ttactcgagg ccaccacgct gcgcgtcgaa ggcacggacg
     3061 accgcacggt cgagcccttc agtaactttg cggtgttcag cgcgcagcag gatctgcggg
     3121 gcgggaacac cggcatcaat ctgatcgcaa cggcggtcaa ccgctcgctg gacgaatgga
     3181 cggaagacgt cctgcattcg tccgcgtatg cggcgggcgc gagcttccgc aatcgatttg
     3241 ccaaccggcg ctacgagctc aatgcctcct ttgcggtgtc acgcgtggcg ggcagcgcgg
     3301 ccgccattcg gcggac
//
LOCUS       DASURG010000464         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_795815, whole genome shotgun sequence.
ACCESSION   DASURG010000464 DASURG010000000
VERSION     DASURG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795815"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFY80_08195"
     CDS             <1..305
                     /locus_tag="VFY80_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007929.1"
                     /translation="GGEKALTALALVFAMFQLNPAPFCLLDEVDAPLDDHNTERFCDL
                     VRKMSQHSQFLFISHNKITMEIAHQLLGITMQEAGVSRVVAVDIDEAMKLAEEAAA"
     gene            322..1416
                     /locus_tag="VFY80_08200"
     CDS             322..1416
                     /locus_tag="VFY80_08200"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX6007930.1"
                     /translation="MSDLQISLLIIGAVVVGGVYLFNWLQERKFRRKLGRAFESQHED
                     VLLEAPGAQGRRVEPQVSWEAAAVEPVAEAPHSERHPTASPAGVSDVAPPFDTETEYV
                     ATMEGHPSIPEAAIDEVLNRAAACGKPCRVVVIRAAGSEWEEPLRRGGEHYVKLAFAL
                     QLVNRSGAMTAAQLALFCDAVKNIATRYAAQVTCPDTQTALGRARDLDKFCSDVDVAI
                     GVNIVAAEGQSFAGQRIRALAEAAGFKLEPDGLFHYRNERRQTLFTLDNHEPAPFLPE
                     QINRLTTTGVTLLLDVPRVADGPGALDRMLEVGRGLARSLGGRLVDDNRVALNEAGIL
                     RIREQLAEICARMEAFGVSAGGSRALRLFS"
     gene            1413..>3289
                     /gene="ligA"
                     /locus_tag="VFY80_08205"
     CDS             1413..>3289
                     /gene="ligA"
                     /locus_tag="VFY80_08205"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX6007931.1"
                     /translation="MTVQHVRERIRRLREEIERHNYQYYVLDQPLVSDADYDRLFREL
                     QQLESQHPELVTPDSPTQRVGAKPLSSFAPLRHRTPMLSLNNAFEAEEVAAFDRRVRE
                     ALGADEVAYSAEPKFDGLAINISYENGLLVHGATRGDGYTGEDVTANLRTIRSIPLKL
                     AAHPPPSRLEVRGEVLMRKADFEALNAEQRTRGEKAFVNPRNAAAGSLRQLDARITAS
                     RRLSFVAYGLGSAEGTPRFRRQSDILDYLAEMRFSIANERAVVHALEGLLAYYERIGR
                     RRGELAYEIDGVVYKVNDLAAQEELGYVARAPRYAIAHKFPAQEAETVVDDIEVQVGR
                     TGALTPVARLKPVFVGGVTVTNATLHNEDEIRRKDVQIGDVVVVRRAGDVIPEVVRVV
                     KERRSRHARPFRMPGRCPVCGSAVQRLEGEAVTRCTAGLFCPAQRKEALLHFASRRAM
                     DIEGLGEKLVDQLVEAGRVKTPADLYKLDVDVLATLDRMAEKSAANVVAAIERSKQTI
                     LARFIYALGIRNVGETTARDLARHFGGIEALMAADSESLQRVSDVGPVVAESIAQFFA
                     EPHNRKVVRQLIEAGVTVENPPAAGRSRSLEGKSFVLTGTLPSLTRDQAKERIEQHGG
                     RV"
BASE COUNT          597 a         1009 c         1126 g          557 t
ORIGIN      
        1 cgggcgggga aaaagcactc acggcgctgg cactggtgtt cgcgatgttc cagttgaatc
       61 ccgcgccgtt ctgcctgctc gacgaggtcg atgcgccgct cgatgaccac aataccgagc
      121 ggttctgcga tctggtgagg aagatgtcgc agcactcgca gttcctgttc atcagccaca
      181 acaagatcac gatggaaatc gcccaccagc tgctcggcat cacgatgcag gaggcgggtg
      241 tatcgcgcgt agtcgccgtg gacatcgatg aggcgatgaa gctcgcagag gaagcggccg
      301 cctgaggcat cctcccacgc catgagcgac ctgcagatca gcctgctcat catcggcgcg
      361 gttgtcgttg gcggcgtcta tcttttcaac tggttgcagg agcggaagtt ccgacgcaag
      421 cttggtcggg cgttcgagag ccagcacgaa gatgtgctgc tcgaagcgcc cggcgcgcag
      481 ggcaggcgcg tggagcccca ggtgtcatgg gaagcagctg cggtcgaacc tgtcgcggag
      541 gcgccgcatt cggagcggca tcccaccgct tcgcccgcag gcgtatccga tgtcgcgccg
      601 ccgttcgaca ccgaaaccga gtatgtggcg acgatggaag ggcacccgtc gattcccgag
      661 gcggcaatcg atgaagtcct gaatcgtgcc gcggcatgcg gtaagccgtg ccgcgttgtc
      721 gtgatccgtg cagccggcag cgaatgggag gagccgctgc gacgcggcgg cgagcactat
      781 gtgaaactcg ccttcgcgct gcagctcgtc aaccggagcg gtgcaatgac tgcggcgcag
      841 ctcgctctct tttgcgatgc ggtgaaaaac atcgctacgc gctatgccgc ccaggtgaca
      901 tgccctgaca cgcagacggc gctcggtcgt gcgcgcgacc tagacaagtt ctgctctgac
      961 gtcgatgtgg cgattggcgt caacatcgtc gcagcggaag ggcagtcgtt cgcgggccag
     1021 cgcatccgcg cgctcgccga ggcggcaggt ttcaagctgg agcccgatgg gctctttcac
     1081 tatcgcaacg agcgccgcca gacgctcttc acgctggata accacgagcc cgcacctttc
     1141 ctgccggagc agatcaaccg gctcaccacg acgggtgtca cgctgctgct cgacgtgccg
     1201 cgcgttgcgg atggccccgg ggccctcgat cgcatgctgg aggtcggacg ggggcttgcg
     1261 cgctcgctcg gcggaaggct cgtggatgac aatcgcgtgg cgctgaacga ggccgggatc
     1321 ctgcgcattc gggagcagct tgcggagatc tgcgcgcgaa tggaagcgtt cggcgtgagt
     1381 gcgggcggtt caagggcgct gcgccttttt tcatgacggt gcagcacgtc cgcgagcgga
     1441 ttcggcggtt gcgcgaggag atagagcggc ataactacca gtactacgtg ctggaccagc
     1501 cgctcgtctc cgacgcggac tacgatcggc tctttcgcga gctgcagcag ctcgaaagtc
     1561 aacatcccga actggtcacg ccggattcgc ccacgcagcg cgtgggagcg aagccgcttt
     1621 cgagttttgc tccgctgcgg caccgcacgc cgatgctgtc gctcaacaac gcgttcgagg
     1681 cggaagaagt cgcggccttc gatcggcggg tgcgcgaagc gctcggcgca gacgaagttg
     1741 cctacagcgc ggaacccaag ttcgacggcc tcgccatcaa tatcagttac gagaacggct
     1801 tactggtgca cggcgcgacg cgcggggacg gctacacggg cgaagacgtt acggccaatc
     1861 tgcgcacgat ccgcagcatt ccgctcaagc ttgcggcgca tcccccgccc tcgcggctgg
     1921 aagtgcgagg cgaggtgctg atgcgcaagg ctgattttga ggcgctcaac gccgaacagc
     1981 gcacccgtgg agaaaaggcg ttcgtcaacc cgcgcaatgc cgccgcgggt tcgctgcgtc
     2041 agctggatgc gcgcatcacc gccagtcggc gcctctcctt cgtcgcctat ggattgggca
     2101 gcgccgaggg cacgccgcgc tttcggcgac agagcgacat actcgattac ctcgcggaaa
     2161 tgcgcttctc gatcgccaac gaacgtgccg tcgtccatgc tctggagggc cttctcgcct
     2221 attacgagag gatcggccgc aggcgcggtg aacttgccta cgagatcgac ggggtggtct
     2281 acaaggtgaa cgatctggcg gcgcaggaag agctgggata tgtcgcgcgc gcgccgcgct
     2341 atgccattgc gcacaagttc ccggcgcaag aggccgagac cgtggtcgac gatatcgagg
     2401 tccaggtcgg gcgtaccggg gcgctgacgc cggtcgcgcg gctgaaaccg gtcttcgtcg
     2461 gcggggtcac ggtgacaaat gcgacgctgc ataacgagga cgaaatccgg cgcaaggacg
     2521 tgcagatcgg cgatgtcgtg gtagtgcgtc gcgcggggga cgtcataccc gaggtcgtgc
     2581 gcgtggtgaa ggagaggcgc tcccgtcacg cgcgcccgtt caggatgccc gggcgctgtc
     2641 ccgtgtgcgg ctcggcggtc cagaggctgg aaggcgaagc ggtcacccgc tgtaccgccg
     2701 ggcttttctg cccggcccaa cgcaaggagg ctctattgca ctttgcctca cgccgtgcca
     2761 tggacatcga agggcttggg gaaaagctcg tggatcagct cgtggaagcc ggcagggtga
     2821 aaacgcccgc cgatctgtac aagctcgatg tggacgtgct tgcgacactc gaccgcatgg
     2881 ccgagaagtc cgcggccaac gtggtggccg cgatcgagcg cagcaagcaa acgatacttg
     2941 cgcgttttat ctatgcgctt ggcatacgca acgtgggtga aacgacggca cgcgatcttg
     3001 cccggcactt cggcggcatc gaggcgctga tggcagcgga cagcgaaagc ctgcaacggg
     3061 tgtccgacgt gggcccggtg gtggcggaaa gcatcgcaca gttcttcgcg gagccgcaca
     3121 atcgcaaggt cgtgcgccaa ctcatcgagg cgggcgtgac agtcgagaac ccgcccgctg
     3181 ccgggcgatc gcgcagcctg gaaggcaaga gtttcgtgct gacgggcact ttgccgtcgc
     3241 tcacgcgcga tcaggcgaaa gagcgcatcg agcagcacgg cggccgggt
//
LOCUS       DASURG010000465         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1512367, whole genome shotgun sequence.
ACCESSION   DASURG010000465 DASURG010000000
VERSION     DASURG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512367"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFY80_08210"
     CDS             complement(<1..683)
                     /locus_tag="VFY80_08210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6007932.1"
                     /translation="MAIHFLVLITVLSHLGFAGSRLAVPLLAVDRGASAFLVGSIVAL
                     YALFPTVLALPAGRMADRMGFRFPLVFGTGAILVAMLVPFVSPTLAALYFSVSVLGLG
                     FMALQLATQTLAGSIAGPGERTRNFSLVSLAFALGNLGGPLMAGVLIDAVGYAWTFFA
                     LAVPLAPATALAVMSRRWLREVHVKAEAARGGALDLLRIAPLRNTLIAGGIIATAWDV
                     YQFLMPLYGR"
     gene            complement(696..1826)
                     /locus_tag="VFY80_08215"
     CDS             complement(696..1826)
                     /locus_tag="VFY80_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007933.1"
                     /translation="MKKLALAGVAAFIAAAAAIWWTGIVSWERHTGYFGPAFSLDGKH
                     LYAVVRETNGFTWGLGWEHFTPPARAYPIADSVRLVRIDITSGQEETLEAWHDTPVLR
                     RVISEYRGRVFNSLRAAVKPDRTGAVRYEVELAIPVVPASEVHRVSGTWSAHNAERRR
                     GTWQREPYASPMLSEPVVSGETEVLPLSGPESYPCALILLDHRTMTARVIVRTRAYAD
                     RYPEGPPLAALVERSRKKDIDRVAALERKHAELVARYRAEGATEIEALLKTGRALEDL
                     GYLPKSPRLIAHRVESGQATRNGLPLFEIAEAEMASGIFPDIEKALAAPGSEVERSLG
                     KYVVHNDYTTSRGLNAHLATGAREFLVRFRGATYRMEIRHAR"
     gene            complement(1834..>2511)
                     /locus_tag="VFY80_08220"
     CDS             complement(1834..>2511)
                     /locus_tag="VFY80_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6007934.1"
                     /translation="KNCSTRIGKGEVVVVCGPSGSGKSTLIKCVNGLEPFQQGEIVVD
                     GKSVGARDTNLPKLRSKIGMVFQHFELFPHMTVTDNLNLAQVKVLGRSREEATAKSMA
                     LLKRVGMEEHAQRFPGQLSGGQQQRVAIARGLAMDPIAMLFDEPTSALDPEMINEVLD
                     VMTALAKDGMTMMVVTHEMGFARRVAGRVVFMDHGEIVEDAPTEEFFGKPRSERAVQF
                     LSKILHH"
BASE COUNT          444 a          812 c          836 g          419 t
ORIGIN      
        1 cgcccgtaga gcggcatgag aaattgatag acgtcccacg cggtcgcgat gatgccgccg
       61 gcaatcaacg tgttgcgcag cggcgcgatc ctcagcagat cgagcgcgcc gccgcgcgcc
      121 gcctccgctt tcacgtggac ctcccgcagc cagcgccggc tcatgaccgc gagcgcggtt
      181 gccggagcga gaggcaccgc tagcgcgaag aacgtccacg cgtaaccgac tgcatcgatc
      241 agcacgccgg ccatcagcgg accacccagg ttgccgagcg caaaggcgag actgaccagg
      301 ctgaagttgc gcgtgcgctc gccgggccca gcgatcgagc cggccagcgt ctgcgtcgcg
      361 agctggagcg ccatgaaccc gaggcccagc accgacacgc tgaaatagag ggcggcgagt
      421 gtcggagaca cgaagggaac cagcatggcg acgaggatcg cacccgtgcc gaagacaagc
      481 ggaaagcgga atcccatgcg gtccgccatc cgcccggccg ggagtgccag caccgtcggg
      541 aagagcgcat agagtgcgac gatggagccg acgagaaagg ccgacgcgcc gcgatcgacg
      601 gcgagaagcg gtacggcgag gcggcttccc gcgaagccga gatgcgacag caccgtgatg
      661 aggacgagga aatggatcgc cacgattact ctgttttagc gcgcatgtcg gatttccatg
      721 cggtaggttg caccgcggaa gcggacgagg aattctcgcg cgccagtggc gagatgcgcg
      781 ttgagcccgc ggctcgtcgt gtagtcgttg tgcacgacgt acttgcccag cgaccgctcg
      841 acctcgctgc cgggcgccgc cagcgctttc tcgatgtcgg gaaagatgcc ggacgccatt
      901 tcggcttccg cgatctcgaa gagcggcagg ccgttcctgg tggcttgccc ggactctacg
      961 cggtgcgcga tgagccgcgg agatttgggc aagtacccga ggtcctcgag cgcgcggccg
     1021 gtcttgagca gcgcctcgat ttcggtcgcg ccttcggcac ggtaccgggc aacgagttcg
     1081 gcgtgcttgc gctcgagcgc cgccacgcgg tcgatgtctt tctttcgcga gcgttccacc
     1141 agcgcggcca gtggcgggcc ttcgggataa cggtcggcgt aggcgcgagt gcgaacgatg
     1201 acccgcgccg tcattgtccg gtgatcgagc aggatcagcg cgcagggata ggattccggt
     1261 ccactcaacg ggagaacttc ggtctcgcct gatacaaccg gctcgcttag catggggctc
     1321 gcataaggct cgcgctgcca tgtaccgcgc cgccgctccg cgttgtgcgc cgaccaggtt
     1381 ccggaaacgc gatggacttc ggaggccggc acgaccggga tcgccagctc gacttcgtag
     1441 cgcacggcgc cggtccgatc cggcttgacc gcggcgcgga ggctgttgaa gacacggccg
     1501 cggtattccg agatcacgcg gcgcagcacc ggggtgtcgt gccacgcttc gagcgtttct
     1561 tcctggcctg aggtgatgtc gatccggacg agccgcacgc tatccgcaat cggataggcg
     1621 cgcgcggggg gcgtgaagtg ctcccacccg agaccccagg tgaagccgtt agtctcgcgc
     1681 accaccgcgt aaagatgctt tccatcgagg gaaaaagccg ggccgaaata gccggtgtgg
     1741 cgttcccagg atacgatgcc ggtccaccag atggcggccg ctgcagcgat gaatgcggca
     1801 acgcctgcga gcgcgagctt tttcaatccg ggatcagtgg tgcaggatct tcgacaggaa
     1861 ttgcaccgcg cgttcggatc gcggcttgcc gaaaaactct tcggtcggtg cgtcctcgac
     1921 gatctccccg tggtccatga acactactcg accggcgacg cgccgcgcga atcccatctc
     1981 gtgggtgacg accatcatcg tcatgccgtc cttcgcgagc gccgtcatca cgtcgagcac
     2041 ttcgttgatc atctcgggat cgagtgcgga agtcggctcg tcgaacagca tggcgatcgg
     2101 atccatggcg agcccgcgcg cgatggcgac gcgctgctgc tgtccgccgg agagctggcc
     2161 gggaaaccgc tgcgcgtgct cttccatccc gacccgcttc aggagcgcca tcgatttcgc
     2221 agtcgcctcc tcgcggctgc ggccgagcac tttcacctgc gcgagattga gattgtcggt
     2281 gacggtcatg tgcgggaaaa gctcgaagtg ctggaacacc atgccgatct tcgagcgaag
     2341 tttcggcaga ttcgtgtccc tcgccccgac cgacttcccg tccacgacga tttcgccctg
     2401 ctggaacggc tccaggccgt tcacgcactt gatgagcgtg gacttgcccg agccggaggg
     2461 tccgcagacc acgaccactt cgcccttgcc gatccgcgtg ctgcagttct t
//
LOCUS       DASURG010000466         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1512437, whole genome shotgun sequence.
ACCESSION   DASURG010000466 DASURG010000000
VERSION     DASURG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512437"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..648
                     /locus_tag="VFY80_08225"
     CDS             148..648
                     /locus_tag="VFY80_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007935.1"
                     /translation="MFALVKGVYQAAIEMEGDDYVPISARTRLGWTGELIALRYGDYR
                     DSLLEIMFDSSTGIVTRLAMPAWKHVNVLPSIEVNSVPGVPVIEPPSQTFEGPSGWKR
                     LDCSADFSLTVHERELTLSIEGGADPIEGIGDGRLVFLVGAAKQLVGLRVRDLTTSET
                     QALRVR"
     gene            777..1649
                     /locus_tag="VFY80_08230"
     CDS             777..1649
                     /locus_tag="VFY80_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX6007936.1"
                     /translation="MTRQAITPLRKRMIEDMEIRRLAAGTQAQYLSVVARFARHFGRS
                     PDQLGYEEVRAYQLHLAQSGLDAGSVNRATTALRFFYCVTMGWRDAPERIPRARTAEK
                     MRGVLTPDEIARMIDAAPGAKYRAALSVAYGAGLRSSEVIALKVSDIDSARMVIRIED
                     GKGRRDRYAKLSPKLLAELRAWWRRAKPRVFLFPSRMSAFDHISTRQFSRACQTAAAT
                     AKLGKSVHPHMLRHSFATHLLDAGVDIRVIQAMLGHKKLETTAIYARVSPKLIQDAAG
                     PFEQLQFRDRTAPA"
     gene            1659..>2236
                     /locus_tag="VFY80_08235"
     CDS             1659..>2236
                     /locus_tag="VFY80_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HEX6007937.1"
                     /translation="MPAQRLEVADIFRACGPAYRTALAGRLSHGQLRVMSAIEACRTA
                     ALGGHVRRCEDCAHVEIAYNSCRNRHCPKCQAAAAYDWLEARRAELLPVAYYHVVFTL
                     PSGIADIAYANKAVVYDALFKAASEALLRIAADPKHLGAKIGVTMVLHAWRSALTHHP
                     HAHCIVPGGGLSADGQEWITCRPGFFLPVRVLS"
BASE COUNT          378 a          728 c          709 g          421 t
ORIGIN      
        1 ctgcgatcat cggcgctgac ccaagaaatg gctggattgg agcccctcaa ccattgcagt
       61 gttgcggcca gctgggcgaa attggcccaa ttcagacatt gagcggcgaa gggcgtaggg
      121 gctagcaggt cgctaggaag cactgcgatg tttgctctag tcaagggtgt ctaccaagcc
      181 gcgattgaga tggagggcga tgactacgtc cccatctccg cgcgcacccg tcttggctgg
      241 acgggtgaac tcattgccct gaggtatggc gactacagag actcgcttct tgaaatcatg
      301 ttcgactcaa gtaccggcat cgtcactcga ttggcgatgc ccgcgtggaa gcatgtaaat
      361 gttttgccat ccatcgaggt gaattctgtt cccggcgtgc ctgtaatcga gccgccctct
      421 cagacatttg aaggcccgtc gggatggaag cgtctggact gctctgcgga tttctctctg
      481 acggtccacg agcgtgagtt gaccctctca atagaaggcg gtgccgaccc aattgaaggc
      541 atcggcgacg ggcggctggt ttttcttgtg ggggccgcaa agcaactcgt gggcctccgg
      601 gttcgcgatt tgactaccag tgaaactcaa gcgctgcgcg tccgttaacg gcgagtgtac
      661 taaaccgctc ccgcggtagg cccctcctga ggggttagcg ctctacgtct gggctggtct
      721 cctgtctgcc ggagcgtccg gattggccgg ttgcgctccg agacgggagc tgtcccatga
      781 cccggcaggc catcacgccg ctgcgcaagc gcatgatcga agacatggag atccgccgcc
      841 tcgcggcggg cacacaggcg caatatctga gcgtcgtcgc caggtttgcg cgccatttcg
      901 ggcgctcgcc cgatcagctg ggctacgagg aggtgcgcgc ctatcagctg cacctagccc
      961 aatcgggcct tgacgccggc agcgtcaatc gcgccacgac ggcgctgcgc ttcttctatt
     1021 gcgtcaccat gggctggcgc gacgcgcccg agcggatccc acgcgcccgc actgccgaga
     1081 agatgcgcgg cgtccttacg cccgacgaga tcgcacgcat gatcgacgcc gcgccgggcg
     1141 cgaaatatcg cgctgctctg agcgtggcgt acggcgccgg tctgcgttcc tccgaagtga
     1201 tcgcgttgaa agtctccgac atcgacagcg cgcgcatggt gatccgcatc gaggacggca
     1261 aaggccgacg tgaccgctac gccaagctgt cgccgaagct gcttgcggag ctgcgcgcct
     1321 ggtggcggcg cgccaagccg agggtgttcc tgtttccgag ccgcatgagc gcgttcgatc
     1381 acatcagcac gcggcagttc agccgcgcat gccagaccgc ggcggcgacg gccaagctcg
     1441 gcaaatccgt gcacccgcac atgttgcgcc actcgttcgc aacgcatctt ctggacgccg
     1501 gcgtcgacat ccgcgtcatc caggcgatgc tcggccacaa gaagctcgag accacggcga
     1561 tctatgcgcg ggtctcgccc aagctcatcc aggacgccgc cggcccgttc gagcagcttc
     1621 agttcaggga tcgaacggca cccgcctgat cggcgctcgt gcccgctcaa cggcttgagg
     1681 tcgcggacat cttccgcgcc tgcgggcccg cttatcgcac cgcgctggcc gggcgcttga
     1741 gccatggcca gcttcgggtg atgtcggcga tcgaggcgtg ccgcacggcc gcactcgggg
     1801 gccatgtgcg ccgctgcgaa gactgtgcgc acgtcgagat cgcctacaat tcctgccgca
     1861 accggcactg tcccaagtgt caggcggcag ccgcctacga ttggcttgaa gcgcgccggg
     1921 ccgagctctt gccggttgcg tactatcatg tcgtgttcac gctgccgagc gggatcgccg
     1981 acatcgccta cgccaacaag gccgttgtct acgacgcgct gttcaaggcc gcgtccgaag
     2041 cgcttttgcg cattgcagcc gaccccaaac acctgggcgc gaaaatcggc gtcacgatgg
     2101 tcttgcacgc gtggcgttca gcgctcacgc atcacccgca tgcgcattgc atcgttcccg
     2161 gcggcggctt gtcggccgat ggccaggaat ggatcacctg ccggccgggc ttcttcctgc
     2221 cggtccgcgt gctttc
//
LOCUS       DASURG010000467         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1410082, whole genome shotgun sequence.
ACCESSION   DASURG010000467 DASURG010000000
VERSION     DASURG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410082"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1875
                     /locus_tag="VFY80_08240"
     CDS             55..1875
                     /locus_tag="VFY80_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007938.1"
                     /translation="MLAGRHAIVATAQTIEYGPWNPGIQSEIPDELLPLSTLFRPENA
                     TRDAAAVEELHRLTGLSMEELALFRAERLVVHELLVRVTADLSVPDGPRVEDLGINFR
                     RMVEIVLERYIAPHMAVIVEEYDALRRTLTAAVDRELEALFAPHRARGTSAMREFLQK
                     VWTAPGAAPDAEDEWARANGLVKTWAATAQSGSTPTGRTACAALNRVVSALLAKYGRV
                     WGDARLISQLAVGMACNEYGSEVVGALIEPHIRRAAEREGYALLPPQSQPVVMNTKGA
                     SGSGKSTIRPLQRRLAGQIGVNWTDFALISPDIWRKYLLDYRALGDAYKYAGAFSGRE
                     LAIIDHKLDRYMARKAERGGMSHLLIDRFRFDSFAPDSEEAGSNLLTRFGSLVYMFFM
                     VTPPHETVERSWKRGLDVGRYKAVDDLLAHNIEAYTGMPQLFFTWALREGKRVHYEFL
                     DNSVPFGERPRSIAFGWNDEMNVLDVSCMLDIDRYRKINVNARNAAEVYPDSGRMAPE
                     SNTQFLVQCARRLPAINFADHDSGRIYARLESGRLVWRDADALSAAMADPETRAGLLA
                     VAPEVHDAYLTQAGAQAGPRLVPRERFHTLGQWGGKNAVD"
     gene            1918..>2181
                     /locus_tag="VFY80_08245"
     CDS             1918..>2181
                     /locus_tag="VFY80_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6007939.1"
                     /translation="MSTLLLVVVASLMTIVAPGTWAQKYPTKPIRLLTPFAPGGGTDI
                     LSRLLGPPLSEALGQPIVVDNRPGAGGTFGALIAVKADPDGYTF"
BASE COUNT          378 a          705 c          731 g          367 t
ORIGIN      
        1 gctggcccaa gcgcggctag cgcacgctga aaaaagtcgt cgccggggaa gccggtgcta
       61 gccggacgac acgcgattgt agccactgcc caaacgatcg agtacggccc gtggaatccc
      121 ggcatacagt cggagattcc cgacgagctg ctgccgctct ccacgctttt ccgtcctgag
      181 aacgccaccc gcgacgctgc ggcggtcgag gagctgcacc ggctgacggg cttgagcatg
      241 gaggagctcg ccctcttccg tgccgagcgc ctcgtcgtgc acgagcttct cgtgcgagtc
      301 acggccgatc tatccgtgcc cgatggcccg cgcgtcgagg acctcggcat caatttccgt
      361 cgcatggtgg agatcgtcct cgagcggtac atcgcgccgc acatggccgt gatcgttgaa
      421 gagtacgacg cgctccggcg cacgctcacc gcagcagtgg atcgtgagct cgaggcgttg
      481 tttgcgccgc accgcgcccg cggcacaagc gcaatgcgcg agttcctgca gaaggtgtgg
      541 actgcgccgg gggccgcgcc ggacgcggaa gacgagtggg cgcgcgccaa cggcctcgtc
      601 aagacatggg cggccaccgc ccaatccggc agtacgccga ccggaaggac agcgtgcgca
      661 gcgttgaacc gggtcgtatc ggcgctgctc gcgaagtacg gccgcgtgtg gggcgacgcc
      721 cggttgattt cacagctcgc cgtcgggatg gcgtgcaatg agtacggcag tgaagtcgtc
      781 ggcgcgctca tcgagcccca tatccggcgt gcggccgaac gcgaaggcta cgcgctcctt
      841 ccgccgcagt cccagccggt ggtcatgaat accaagggcg cctccggttc cggcaagagc
      901 acgatccggc cgctccagcg ccggcttgcg ggccagatcg gcgtcaactg gacggacttt
      961 gcgctcatca gcccggatat ctggcgcaag tatctgctcg attaccgcgc gctcggcgac
     1021 gcgtacaagt atgccggggc attcagcggg cgcgagctcg cgatcatcga ccacaagctc
     1081 gatcgctaca tggcgcgcaa ggcggagcgc ggcggcatgt cgcatctgct gatcgaccgc
     1141 tttcgcttcg acagcttcgc gccggattcc gaggaggcgg ggagcaacct tctcacgcgc
     1201 ttcggcagcc tggtctacat gttcttcatg gtgacgccgc cacacgaaac ggtcgagcgt
     1261 tcgtggaagc gcgggctcga cgtcgggcgc tacaaggcgg tggatgatct gctcgcgcac
     1321 aacatcgagg cctacaccgg catgccgcag cttttcttca cgtgggcgct gcgggaaggc
     1381 aagcgcgtcc actacgagtt tctggacaac agcgtgccgt tcggcgagcg gccgcgcagc
     1441 atcgcgttcg gctggaacga cgagatgaac gtgctcgacg tgagctgcat gctggacatc
     1501 gaccgctacc gcaagatcaa tgtcaacgcc cggaatgccg ccgaggtata tccggacagc
     1561 ggccggatgg cgccggagag caatacgcag ttcctcgttc agtgtgcgcg ccggcttccc
     1621 gcgatcaact tcgcggatca cgacagcggc cgcatctatg cgcggctcga gtccggtagg
     1681 ctggtttggc gcgacgccga tgcactgtcg gcagcgatgg cggatcccga gacgcgcgcc
     1741 ggactccttg ccgttgcgcc cgaagtccat gatgcatatc tgacccaggc aggcgcgcaa
     1801 gccgggcctc gtctcgtgcc gcgcgagcgg tttcatactt tggggcaatg gggaggaaaa
     1861 aacgctgtag actgagcgga cgaaggagga tgcggttttc cacgaggagg aacgaacatg
     1921 agcaccctat tgctggtggt ggtcgcgtcg ctgatgacga tcgttgcgcc cggaacgtgg
     1981 gcgcagaagt atcccaccaa gccgatccgg ctcctcacgc cgtttgcgcc cggcggcggc
     2041 acggatattc tttcgcggct gctcggcccg ccgctctccg aggcgctcgg gcagccgatc
     2101 gtcgtcgaca atcgccccgg cgccggaggt acgttcggcg ccctgatcgc cgtgaaagcg
     2161 gatcctgatg gctacacgtt c
//
LOCUS       DASURG010000468         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1205071, whole genome shotgun sequence.
ACCESSION   DASURG010000468 DASURG010000000
VERSION     DASURG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205071"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFY80_08250"
     CDS             complement(<1..318)
                     /locus_tag="VFY80_08250"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEX6007940.1"
                     /translation="MNTDQLIAMLATSATPVEPNAAARRYAAATGWSALATVSLMAAM
                     LGVRADLADAVMLPMFWTKVVFVAGLAGAGVVAAMRLSRPGVPLGSVPGVLIALVVAM
                     WALA"
     gene            complement(315..860)
                     /locus_tag="VFY80_08255"
     CDS             complement(315..860)
                     /locus_tag="VFY80_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6007941.1"
                     /translation="MIENEAVLRNLLIRGLSGDTHAYHAFLKAVSVYLRSFLRRRLAN
                     LPDELEDVVQDSLLAIHQQRHTYDTGQPVTAWVHAIAKYKLVDLLRRRGSREAMTDPL
                     EDDNDLVSPMDSEAADARRDLAKLLNLLPDRQRLPIVHVKLEGLSVAEAASVTGMSES
                     AVKIGIHRGLKALAARIKGAT"
     gene            1087..1242
                     /locus_tag="VFY80_08260"
     CDS             1087..1242
                     /locus_tag="VFY80_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007942.1"
                     /translation="MSRVAAVLFFLGFVTAGNPVLAQDAMQKDGMTKETTTTTTTDSM
                     GHDGMRK"
     gene            1271..1504
                     /locus_tag="VFY80_08265"
     CDS             1271..1504
                     /locus_tag="VFY80_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007943.1"
                     /translation="MLKRSIVVLTLAGGLLGAQGALAYGHQATGSEEASGLRGYLDGR
                     ITSEINATHRYSSEAQGWGRSDSSPFPWDNSGD"
     gene            1571..2200
                     /locus_tag="VFY80_08270"
     CDS             1571..2200
                     /locus_tag="VFY80_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX6007944.1"
                     /translation="MDGQVVKVHSPAVRIAHWVNAFAILIMVLSGWRIYNAAPLFPFS
                     FPRALTLGGWLAGALQWHFAAMWLLVLNGLCYITYGICSGHFRHRLFPVTPGAVARDV
                     KAALHGRLAHDDLRVYNAAQRAAYIAAIVLGIVLVVSGLVIWKPVQFHTLGYFIGDYE
                     GARYLHFFSMALLVGFVAVHVAMVLLVPRTLTAMVTGRTYFKAAQGGPQ"
     gene            2197..>2646
                     /locus_tag="VFY80_08275"
     CDS             2197..>2646
                     /locus_tag="VFY80_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX6007945.1"
                     /translation="MKRSPGMGQSLEAFSRPQIENIERRLFMRRGLSLGALALLSGCD
                     VTDTEAVQKALFAMSRWNDRVQGWLFDPRKLAPEFPETAITDPFPFNAFYGESKVPKL
                     DGNRYTLQVGGLVRERRPWALPELYALPQASQVTRHICVEGWSAIGKW"
BASE COUNT          490 a          790 c          810 g          556 t
ORIGIN      
        1 cgcgagcgcc cacattgcga ctaccagtgc gataagcacg cctggcaccg agccgagcgg
       61 tacgccgggc cgcgagaggc gcatcgcggc gaccaccccg gccccggcga gaccggccac
      121 gaacacgact ttggtccaga acatcggcaa catgacggcg tccgcaagat ccgcccgcac
      181 ccccagcatg gccgccatca gtgacaccgt agccaacgca ctccatcccg tggctgcggc
      241 gtagcgccgt gccgcagcgt tcggttccac aggtgtggcc gacgttgcga gcatcgcgat
      301 aagctggtct gtattcatgt tgcgcctttg attcttgcag caagcgcctt gaggccccga
      361 tgaatgccga tcttgacggc cgattcggac atgcctgtca cgctcgccgc ttccgcgacc
      421 gacaatcctt ccagcttgac gtgaacgatg ggcagccgct gccgatcggg aagcaggttc
      481 agcagcttcg ctagatcgcg acgcgcatcg gcagcttcgc tatccatggg ggacacgaga
      541 tcgttgtcgt cttccagcgg atcggtcatg gcttcccgac tgccacgccg gcgcagcaaa
      601 tcaacgagct tgtatttcgc aatggcgtgc acccaggcgg tcacgggctg accggtgtcg
      661 taggtatgac gctgctggtg tattgccaga agtgagtcct gcactacgtc ctcgagctca
      721 tcgggtagat tagcgaggcg tcttctcagg aaggagcgca ggtacacgct caccgctttc
      781 aggaacgcat gataagcgtg agtgtcaccg ctcagtccgc gaatcagcag attccggagt
      841 actgcctcgt tctcgatcaa agtctttctc ttcttttgtg gttattcggt tcgtcgcggc
      901 gttcggttac accttacaag aaagatattt ccaagccggg ctctgtaacc gccgaggctc
      961 tttcctcgaa ttagctctcc tgaacatcgt attgaagcac ccagagctcg aaggcagccg
     1021 ggcttcactg ggaagctccg aaaggagcaa gcgctgccgg tcacttccaa actttaggag
     1081 caccacatga gcagagttgc agcagttctc ttttttcttg gctttgtgac ggcgggaaac
     1141 cccgtcctgg ctcaagacgc gatgcagaaa gacggcatga ccaaagagac caccaccacg
     1201 accaccacgg actcgatggg tcacgacggg atgcggaagt aaaccaacct cacttatagg
     1261 agattacatc atgttgaaac gtagcattgt cgtgctgact cttgcgggcg ggctgctcgg
     1321 ggcgcagggt gccttggcct atgggcatca agccaccggc agcgaggaag cgagcggatt
     1381 gcgcgggtat ctcgatggtc ggatcacgag cgagatcaac gcgacgcacc ggtattcgag
     1441 cgaagcgcag gggtggggaa ggtccgattc ttctccgttt ccctgggaca acagcggcga
     1501 ctgatggtcg cgtctgcgta tcaaccggct ccgattcatt cggggccggt tttcatttca
     1561 ggagaccgtc atggatggcc aagtcgtgaa agtgcactcg cctgcagtgc gcatcgcaca
     1621 ctgggtgaat gcatttgcga tactgatcat ggtgttgtcg ggctggcgca tctacaacgc
     1681 cgcgccgctt ttcccattct cgtttccgcg tgcgctcacc ctcggcggct ggctcgcggg
     1741 ggcgctgcag tggcacttcg ccgcgatgtg gctcttggtg ctcaacgggc tttgttacat
     1801 cacttacggg atttgctccg ggcactttcg acaccggctg tttccggtca cgccgggtgc
     1861 agtggcgcgc gacgtgaagg cggcgctgca tggccgactt gcgcacgatg atttgcgtgt
     1921 ctacaacgcc gctcagcgtg ctgcctatat cgccgccatc gtgctcggca tcgtgctcgt
     1981 cgtctccggg ctcgtgatct ggaagccggt gcagttccac acgctgggat acttcatcgg
     2041 tgattacgag ggggcgcgct acctgcactt cttctcgatg gcgcttctcg tcgggttcgt
     2101 cgccgttcat gttgcaatgg tgctgctcgt gcctcggacg ctcacagcga tggttactgg
     2161 gcgcacctat ttcaaggcag cgcagggagg cccgcaatga agcgatctcc tggaatggga
     2221 caaagtctcg aggcattctc gaggccgcag atcgagaata tcgagcgccg gcttttcatg
     2281 aggcggggat tgagcctggg cgcgctcgct cttctatccg gctgtgatgt gaccgacacc
     2341 gaagcggtac agaaggcgct gttcgccatg tcgcgctgga acgaccgcgt gcagggctgg
     2401 ttgttcgacc cgcggaagct tgcgcccgag tttcctgaaa ccgcaatcac cgatccgttt
     2461 ccgttcaacg ccttttatgg cgagagcaag gtgccgaaat tggatggaaa ccgctacacg
     2521 ctccaggtag gcggtctggt ccgggagcgc aggccgtggg cgcttcccga gctctacgcg
     2581 ctccctcaag cgtcccaggt cacgcgccat atctgcgtgg agggctggag cgcgatcggt
     2641 aaatgg
//
LOCUS       DASURG010000469         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_287497, whole genome shotgun sequence.
ACCESSION   DASURG010000469 DASURG010000000
VERSION     DASURG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287497"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VFY80_08280"
     CDS             complement(<1..159)
                     /locus_tag="VFY80_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6007946.1"
                     /translation="MALRIGDDAPDFTAETTQGTIRFHEWIGEGWAILFSHPKYFTPV
                     CTTELGYMA"
     gene            complement(544..744)
                     /locus_tag="VFY80_08285"
     CDS             complement(544..744)
                     /locus_tag="VFY80_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007947.1"
                     /translation="MLDMLPPLPAALPLLPAVLPADGLVADGLLDLSVPAALAAKGRA
                     NAAATATAIRVFELRMVFSSQK"
     gene            743..982
                     /locus_tag="VFY80_08290"
     CDS             743..982
                     /locus_tag="VFY80_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007948.1"
                     /translation="MKRLDTNNDGSVSRDEAKKDTNISKRFKELDKNNDGKLSSTELS
                     AAEATGAGGSAKSGSTAGKASSAQGSATEGPKKGN"
     gene            complement(1090..1815)
                     /locus_tag="VFY80_08295"
     CDS             complement(1090..1815)
                     /locus_tag="VFY80_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6007949.1"
                     /translation="MVNREEILKRVQKALEHEPRINLHRHPVRIDYSDGAVILEGEVR
                     DPAAKKLALEKAAAVGDVRGVIDRLRVAPSERRGDGAIRTSLANRLLQESEFRNCAIR
                     ARTKGRIETLREVASDWGGDIEIGVEEGVVTLEGRVLSLSHKRAAGVLAWWTPGCRDV
                     VNALAVEPPEDDHDDEIVDALKLVLEMDPLVQADRIHATCRGAVVRLEGVARTEEERR
                     QAEYDAWCLFAVDGVENRIEVRT"
     gene            complement(1882..2379)
                     /locus_tag="VFY80_08300"
     CDS             complement(1882..2379)
                     /locus_tag="VFY80_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007950.1"
                     /translation="MARQKTATRSADGFDHTFRAIHDHEIPWESDDVRGILKLPPGVK
                     VKILNYDPVMKRIDMKQRFPAGYVEPRHTHTSWHSILVTKGRMCVAGKDLRPGDYVFG
                     WDIPHGPYEYPDGCEVFIVYMGDATHIWEKEDLLKHKNIWKPKTKVGKQGVAKHLQKR
                     KAGQI"
     gene            2528..>3142
                     /locus_tag="VFY80_08305"
     CDS             2528..>3142
                     /locus_tag="VFY80_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX6007951.1"
                     /translation="MRSVLARAAAAAGILVIAAGCAHVGGSKVDPDGVYNAILQDDVA
                     QIRALVHSGTLAVNQSIPVSGYREGTPLLTVAARSASLAVIRYLLSAGADVNARTPVN
                     ETALMLAAYFHDEDSARSANARVRHDEAVRILLDAGADIENDPYHYTPLSYAAYQGND
                     IAVGMLLAHGARVDADAENGSTYINTPLMMAAIQGHESTVLQLLR"
BASE COUNT          577 a         1023 c          958 g          584 t
ORIGIN      
        1 ggccatgtag ccgagctcgg tcgtgcagac cggcgtgaaa tacttggggt gggaaaacaa
       61 tatcgcccag ccctctccga tccactcgtg aaaacgtatc gtgccctgcg tcgtctccgc
      121 cgtgaaatcc ggcgcgtcgt ctccaatcct cagtgccata tcgtcctcct gatgtaagaa
      181 tgaaacgccg cgacgcgaat gcagctgcgt ggtcgagcga gcagtggtgg ggtcgtggcg
      241 ctgggcgagt agggactcgc ccggccgcca ttatcgcgcg ctcggcgcac ggcgacaaaa
      301 gaagcccggc tcggccgtat acggcttgcc ggcctgttgg cgcttgccga caagcgattc
      361 agtacgcttt tccgcgactt agcgcctccg agtgccggcg gctgtatcca agcggcgaca
      421 tcggcggccg agcgacccgg ctatcgcgct gaaattgaaa tgcaatttca ctggcacggc
      481 gcttgctccg cctcccgagg cccgcgtcgt cgggcacgtt attcgagcgt ccaccaagtt
      541 tttctacttc tgagaggaaa agaccatgcg caactcgaaa accctgatcg cggtggcggt
      601 tgctgccgcg tttgccctgc ccttcgcagc gagcgctgca ggcaccgaca agtcgagcag
      661 cccgtccgcg accagcccgt ccgccgggag caccgcgggc aagagcggca gcgcggccgg
      721 caacggtggc agcatgtcaa gcatgaagcg gctcgatacc aacaacgacg gcagcgtgtc
      781 gcgcgacgaa gcgaagaagg acaccaatat ttccaagcgc ttcaaggaac tcgacaagaa
      841 caacgacggc aagctttcat cgactgaact gagcgcggcg gaggcaaccg gcgccggcgg
      901 ctccgccaag agcggcagca ccgcgggcaa ggcaagcagc gcccaaggca gcgccactga
      961 aggtccgaag aaaggcaact gatccgggag cgcggtctta aacaaaccgc gatcccagaa
     1021 atcgcgatcg ccggatggcg attgcccaac gtcccgtcac gtcggaaaaa accggtgggc
     1081 ggggcgtagc tacgtacgga cttcgatccg gttctcgacg ccgtccaccg cgaagagaca
     1141 ccacgcatcg tactccgcct gacgtctttc ctcttcggta cgcgccacgc cctcgagcct
     1201 caccacggcg ccccgacagg tagcatgaat gcggtccgcc tgcaccagcg gatccatctc
     1261 gagaacgagc ttgagcgcgt ccacgatttc gtcgtcgtga tcgtcctcgg gaggctccac
     1321 ggccagggcg ttcacgacgt cgcggcatcc cggagtccac cacgcgagca caccggcggc
     1381 gcgcttgtgc gagaggctca gcacccgccc ttccagcgtc accaccccct cctcgacgcc
     1441 gatctcgatg tcgccacccc aatcgctcgc gacctccctc agcgtctcga tccgtccttt
     1501 ggtgcgcgcg cgtatggcgc agtttcgaaa ttcgctttcc tgaagcaggc gattggcaag
     1561 cgaggtgcgg atcgcgccat cgccccgacg ttcggacggc gcgacgcgaa gccggtcgat
     1621 gacgcctctc acatccccta cggcggccgc tttttccagc gcgagcttct tggccgcggg
     1681 gtcgcgcact tcgccttcga gaatcaccgc gccgtcgctg tagtcgatgc gcaccggatg
     1741 gcgatgaaga ttgatgcgtg gctcgtgctc cagcgccttc tgcacccgct tgaggatctc
     1801 ttcccgattg accatggtct tactcctttg atgggtggag tgccgcacgg ccgggatgtt
     1861 ccgcaagaat cgcgcgcgcc gctagatctg gcccgccttg cgcttctgca gatgcttcgc
     1921 tacgccctgc ttgccgacct tggtcttcgg cttccagatg ttcttgtgct tgaggagatc
     1981 ctctttttcc cagatgtgcg tcgcgtcgcc catgtagacg atgaagacct cgcagccgtc
     2041 cggatactcg taagggccgt ggggaatgtc ccagccgaag acgtagtcgc ccggacgcag
     2101 gtccttgccc gcgacgcaca tgcggccctt ggtcacgagg atggaatgcc agctcgtatg
     2161 ggtatggcgc ggctcgacgt atccggcggg gaagcgctgt ttcatgtcga tgcgtttcat
     2221 gaccggatcg tagttgagta tcttcacctt gaccccgggc ggaagtttga ggatcccgcg
     2281 gacatcgtcg ctttcccacg gaatctcgtg atcgtggatt gccctgaagg tgtgatcgaa
     2341 gccgtccgcg cttcgggtcg cggttttctg ccgtgccatc ttccctctcc ttttgagtcc
     2401 agcgatgaac cgaacggcat cataccgcat accgcgcagg cggttgacga ccggcgcaag
     2461 ctgatgcact gttctgctgc gcgcaagcaa atgtcttacc ggagaagaat aaaggaagga
     2521 tgacatcatg aggagcgttc tcgcccgggc cgctgccgcg gctgggatcc tggtgatcgc
     2581 ggcaggttgc gcgcatgtcg gtggaagcaa ggtggacccc gacggcgtct acaacgcgat
     2641 attgcaggac gacgttgcgc agatccgcgc gctcgtgcac tccggcacgc tcgccgtcaa
     2701 ccagtccatc ccggtgtcgg ggtatcgcga aggcacaccg cttctgacgg ttgccgcgcg
     2761 atccgcatcg cttgcggtga ttcgttacct cttgtcggca ggagccgacg tcaacgcccg
     2821 cacgccggtc aacgagaccg cacttatgct cgccgcatac ttccacgacg aggatagcgc
     2881 gcgcagcgcc aacgcccggg tgcggcacga tgaggcggtt cgtatccttc tcgatgcggg
     2941 tgcggatatc gaaaacgatc cctaccacta cacgccgctt tcctatgccg cctatcaggg
     3001 caacgacatc gcggtaggca tgctgcttgc gcacggcgcc cgcgtagatg ccgatgcgga
     3061 aaacgggtca acctacatca atacgccgct catgatggct gcgatccagg ggcatgagag
     3121 cactgtgctg cagttgctcc gc
//
LOCUS       DASURG010000470         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_388696, whole genome shotgun sequence.
ACCESSION   DASURG010000470 DASURG010000000
VERSION     DASURG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388696"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFY80_08310"
     CDS             complement(<1..500)
                     /locus_tag="VFY80_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6007952.1"
                     /translation="MWIYVVFLIVLCNMTVFRGSKVLVSLFAIELGAPQFYIGVLIAL
                     YSLFPMMLALYAGKLSDRLGVRYPMIAGSSGLALGLSLPYFAPGLPALYASAAMIGAS
                     HVFYNVSVQNLVGALSGTAERTRNFTNYGLVMAAGGFLGPLIAGFSIDHFGHAAGYLV
                     LAGGPL"
     gene            648..1940
                     /locus_tag="VFY80_08315"
     CDS             648..1940
                     /locus_tag="VFY80_08315"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6007953.1"
                     /translation="MADVTITVSKHHASMLRNTLRALRHRNFRLFIEGQMCSLIGYWM
                     QNIAQSWLLYRLTESTTLLGMLAFAGSVPILLLAPLAGLWSDRANLHRLMFTIQVLEM
                     VQAAALAALAVSGAIEPWHIVALSMLLGVLVAIELPVRHAYLLELVGDKADLPNAVAV
                     TSMMANSGRLIGPALAGVAIGWIGEAGCFIINTFSYIAVVVTFLMMRVAPRARPETHS
                     SVMEGLSEGLRYAWHSIPIRLLLLMLAITGFLATPYAALMPALTREVLHGGAQEMGFL
                     VGAAGLGGLAGTVYLASRRNVRGLVTIIIYAILSAGTALAALSWTSSLWLALPLLVVI
                     GFCILVTSVSVNMILQTIVDDDKRGRVMSLYTAAFLGISPFGALAAGATADWIGVTAT
                     LTVSGACCVVGAFYLASRLPQIRAHIRPIYGRLGITRR"
     gene            complement(1927..>2966)
                     /locus_tag="VFY80_08320"
     CDS             complement(1927..>2966)
                     /locus_tag="VFY80_08320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6007954.1"
                     /translation="VAFGYLEGGAEDHLTYHANRAALDRIRFVPRTLVDVSCRSQNVT
                     VFGRPYSSPFGIAPTGAAGIYCRGAEIALARGARDANVPFVLSTHSFVPLAQVCRETG
                     GAPWYQMYLQPQRADTEAVVHAARDAGCEVLVLTVDAATGGNREYNYRNGFSIPVRIT
                     RRTGFDGLCHPSWLADVYWRTLRGRNLIEWTVPHDRGTWSDLSWLRRVWPRKLIVKGV
                     LTVEDARLAEAHGADGVWVSNHGGRQLDGALASMETLPHIAAAVGHRLTIMVDSGFRR
                     GTDVVKALALGADMVFFGRGALYGAAAAGEAGVRRALDLIRIEVDRVLALLGCNGVGD
                     LGAQHVRVSAG"
BASE COUNT          504 a          978 c          946 g          538 t
ORIGIN      
        1 atcagcggcc cgccggcaag cacgaggtag cccgcggcgt gaccgaagtg gtcgatcgaa
       61 aaaccggcaa tgagcgggcc aaggaatcca ccggcggcca tgacgagccc gtagttggtg
      121 aagttgcgtg tgcgttccgc agtgccgctc agcgcgccga cgagattctg taccgagacg
      181 ttgtagaaca catgggaggc gccgatcatc gcggccgacg cgtagagcgc cggcagcccg
      241 ggcgcgaaat atggcagcga aagtccgagc gcgagaccgc tcgatcccgc gatcatggga
      301 tagcgcacgc cgagccggtc cgaaagtttt ccggcataga gcgcgagcat catcggaaag
      361 agcgaataga gcgcgatcag aacgccgatg tagaactgcg gcgcgccgag ctctatggcg
      421 aacagcgaca ccagcacctt gctgccgcga aagaccgtca tgttgcagag gacgatcaga
      481 aaaacgacgt agatccacat gctaccggtg cggcacgggg accacgatgt gggatggcat
      541 cacgggttcg ttcgtattcg gcggccgcgt gcagcgcgct tcgggcgcgc agcatcgatg
      601 ccgtccgatt ttaccggact cgcgtacgcc tcgcgcgcgg attccgcatg gcggacgtta
      661 caatcacggt ttcgaagcac cacgcctcaa tgctgcgcaa tactctgcga gcgctccgtc
      721 accgcaattt ccggctcttc atcgagggcc agatgtgctc gctcatcggc tactggatgc
      781 agaacatcgc gcagagctgg ctgctgtatc gcctgaccga atcgacgacg ctgctcggca
      841 tgctggcgtt cgctggcagc gtgccgatcc tgctgctagc gccgttggct gggttgtggt
      901 ccgaccgcgc gaatctccat cgcctcatgt tcacgatcca ggttctcgag atggtccagg
      961 ctgccgcgct tgcggcgctc gcggtctcgg gagccatcga gccgtggcac atcgtggcgc
     1021 tgtcgatgct gctcggcgtg ctggtcgcga tcgaacttcc cgtgcgccat gcctatctgc
     1081 tggagctcgt cggcgacaag gccgatcttc cgaacgccgt cgcggtgaca tcgatgatgg
     1141 cgaacagcgg ccggttgatc ggccctgcgc tcgccggcgt tgcgatcggc tggattggcg
     1201 aggctggctg tttcattatc aacacgtttt cctacattgc cgttgtcgtc acgttcctca
     1261 tgatgcgcgt tgcgccgcgg gcgcggccgg aaacacattc ttcggtgatg gaaggcttga
     1321 gcgaaggcct gcgctacgcc tggcattcga tcccgatccg gctcctgctg ctcatgctcg
     1381 ccataacggg ctttctcgca acaccctatg ccgctctcat gccggcactg actcgtgagg
     1441 tcttgcacgg tggtgcgcaa gagatgggat ttctcgtcgg agccgcggga ctgggcggcc
     1501 tggcgggcac ggtctatctc gcctcgcggc gcaatgtgcg cgggctcgtc acgatcatca
     1561 tctacgcgat tctgagtgcg ggcacggcgc tcgcggcgct ttcgtggacc tccagcctgt
     1621 ggctggcgtt gccgctactg gtcgtgatcg gcttctgcat tctcgtcaca tcggtctcgg
     1681 tcaacatgat cctccagacc atcgtcgatg acgacaagcg cgggcgcgtg atgagcctct
     1741 acaccgcggc gtttctgggc atttcgcctt tcggcgcgct cgctgcgggc gcgacagcgg
     1801 actggatcgg cgttaccgct acgttgactg taagcggcgc ctgctgcgtg gtgggcgcct
     1861 tctatctcgc ctcgaggctc ccgcagatcc gcgcccacat ccgcccgata tacggccggc
     1921 tcggcatcac ccggcgctga cccggacgtg ctgcgctccg agatcgccga cgccgttgca
     1981 tccgagcagc gccagcacgc ggtccacctc gatgcggatg aggtcgagcg cgcgccggac
     2041 gccggcttct cccgcggccg ctgcgccgta gagtgccccg cgcccgaaga acaccatatc
     2101 ggcacccagc gcgagcgcct tgacaacgtc ggtgccgcgc cggaagccgc tgtccaccat
     2161 gatcgtcagg cggtgtccga ccgcagcggc gatatgcggc aatgtttcca tcgacgccag
     2221 tgcgccgtcg agctggcgtc cgccgtgatt cgatacccat acaccatcgg cgccatgcgc
     2281 ttcggcaaga cgcgcatcct ccacggtcag cacgcctttc acgatgagct tccgcggcca
     2341 tacacgccgc agccaggaaa ggtcgctcca agtgccccgg tcgtggggca cggtccattc
     2401 gatgagattc cggccgcgca gcgtccgcca gtacacgtct gcgagccagc tcgggtggca
     2461 caatccgtcg aagccggtgc gccgggtaat ccgcaccgga atgctgaaac cgttgcggta
     2521 gttgtattca cggttgccgc cggttgcggc atcgaccgtc agcaccagaa cctcgcagcc
     2581 ggcatcgcgc gccgcatgta ccaccgcttc ggtgtccgcc cgctgcggct ggaggtacat
     2641 ctgataccac ggcgcgccgc ccgtttcccg acacacctgc gcgagcggca cgaaagaatg
     2701 cgtggacagc acgaacggca cgtttgcgtc cctcgccccg cgtgcgagcg cgatctccgc
     2761 cccgcggcag tagattcccg ctgcaccggt cggcgcgatg ccgaagggac tcgagtaggg
     2821 ccggccgaaa accgtcacat tttgcgagcg acacgacacg tccacgagcg tgcgcggcac
     2881 gaatcgaatg cgatcgagcg ccgcccggtt ggcatggtat gtgaggtgat cttcggcacc
     2941 gccttcaagg taaccgaatg caacgc
//
LOCUS       DASURG010000471         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1102963, whole genome shotgun sequence.
ACCESSION   DASURG010000471 DASURG010000000
VERSION     DASURG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102963"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /locus_tag="VFY80_08325"
     CDS             <1..2246
                     /locus_tag="VFY80_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6007955.1"
                     /translation="NTLRAMGEEYAREVAAGEFSEQAFHARIHQRAARLPQLSSLLVI
                     QADGKIAAHSQIYPSPSVDFVDRDYFHAQREAKEDALYFGVPVKGRIINEWLNPVSRR
                     VIGPGGAFLGVAVAAINIPYFEEIYRSVELGPSGHVFLFRDDGVLLTSFPRREADFGQ
                     SFRHHPLFSSISATTGIVYARGVMDEASRLIGYHKVAGYPLVAVVASTEDYVLREWRQ
                     QSLYAAASALVAMAVILAGAFLIAHQVRVAQELAGEVAESEYRWLTAMEAAGHGVWEW
                     DVTTGRTFRSRQYHRMLGYDDQEIPNTRESWLKLLHPEDRMVAEEADRACLEGRVNAF
                     SNELRLKCKDGSWKWMLNRGTVVSRDPHGGALRVLGTLTDVTETHEAQQRLRDSAARL
                     NAIVESAMDAIITIDEHHTIVLFNKAAEKIFRCPARGAPGEEAIGSKLDRFIPERYRT
                     AHRAHIERFGRTGTTMRRMGENIVLAGMRADGEEFPIDASISQVEVAGRRYYTVILRD
                     ITERKRSADALHASHRELRELYGVMNEVREAERLRIARELHDELAQWLTALKMDVSWI
                     AARLPKELPRLMDKTAKMKEVVDTTVAAVRRIASDLRPVMIDDLGLIPAIEHLLHDFS
                     QRSGVVVSLDARTDTVDLADPIATAVYRMVQEALTNVARHAEATRVEVSFAIEGENLV
                     AKVCDNGRGIDEAAMRKGKSYGVLGIRERAQTLGGSARIYKPDGGGTTVEITIPLRAQ
                     ARMESVA"
     gene            2243..2893
                     /locus_tag="VFY80_08330"
     CDS             2243..2893
                     /locus_tag="VFY80_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6007956.1"
                     /translation="MIRVVLADDHRMVRTGLKEILADTGDIEVVGEATNGHEVMARVR
                     ELLFDLLVLDMSMPGRSGIDLIRQVKSEKPALRILVLSMHSEEQYAVRALKAGAAGYL
                     TKESAADELVAAIRRIAGGGAYVSPETAQRLALGLEAGRERPPHESLSDREMQVFHMI
                     AAGKSVTDIADTLTLSVKTVSTHKTRIMQKLNAHNQTDLIRYAIRHKLVDDPAESE"
BASE COUNT          574 a          913 c         1012 g          495 t
ORIGIN      
        1 acaacacgct gcgggcgatg ggcgaagagt atgcgcggga agtggccgcc ggggaatttt
       61 cggagcaggc gttccatgcg cgcatacacc agcgcgcagc ccggctcccg caattgagct
      121 cgctgctcgt gatccaggcc gacggcaaga ttgccgcgca ctctcagatc tatccctccc
      181 cgagcgtcga tttcgtcgac cgcgattact tccatgcaca gcgcgaggca aaagaggacg
      241 cactctattt tggcgtcccg gtgaaaggtc gcatcatcaa tgagtggttg aatccggtga
      301 gccggcgcgt gatcggcccg ggcggcgcgt ttcttggcgt agcagtcgcg gccatcaaca
      361 ttccgtattt cgaggagatt taccgctcgg tggagctcgg cccgtcaggc cacgtatttc
      421 tgttccgcga cgacggcgta ctcctcactt cgttcccgcg gcgggaggcc gattttggcc
      481 agtcgttccg gcaccacccg ctgttctcga gcatctcggc gaccaccggt atagtttatg
      541 cgcgaggtgt gatggacgag gcgtcacggc tcatcggtta tcacaaggtc gctggctacc
      601 cgctcgtggc ggtcgtcgcc tctacggaag actacgttct gcgcgaatgg cggcagcagt
      661 cgttgtacgc ggcggcaagc gcgctcgtcg cgatggcagt gatcctcgcc ggagcgttcc
      721 tcatcgcgca tcaagtgcgc gtggcgcagg agctcgccgg cgaggtggcg gagtcggaat
      781 accgctggct aaccgcgatg gaggcggcgg gacacggcgt gtgggaatgg gacgtgacga
      841 ccggcaggac gtttcgctca cgccagtacc atcgaatgct gggctatgac gatcaggaga
      901 ttcccaatac gcgcgagagc tggctcaagc tgctgcatcc cgaggaccga atggtggccg
      961 aggaggcgga tcgcgcgtgc ctcgaagggc gcgtgaacgc gttttcgaac gagcttcggc
     1021 tgaagtgcaa ggacggaagt tggaagtgga tgctcaaccg cggcacggtg gtgagccggg
     1081 acccgcatgg cggggcgcta cgcgtgctgg ggacgcttac ggacgtcacg gagacgcacg
     1141 aagcccagca gcgtctgcgc gacagcgccg cgcgtctgaa cgcgatcgtt gaatccgcga
     1201 tggatgcgat catcaccatc gacgagcacc acaccatcgt gctcttcaac aaggcggccg
     1261 agaagatatt tcgctgtccc gcgcgcggtg cgccgggcga ggaggcaatc ggctcgaagc
     1321 tcgatcgctt cattccggag cggtatcgca cggcccatcg cgcgcacatc gagcgcttcg
     1381 gccgaaccgg cacgacgatg cggcgcatgg gcgagaacat cgtgctggcg ggaatgcgcg
     1441 cggacggcga ggaatttccg atcgatgcgt ccatttccca ggtggaagtg gccggacggc
     1501 gctactacac cgtcattctg cgcgatatca ccgagcgcaa gcgctcggcc gatgctttgc
     1561 acgcatccca ccgtgagctg cgcgagctct acggcgtgat gaacgaggtg cgggaagcgg
     1621 agcgtctgcg catcgcgcgc gagctgcacg acgagcttgc acagtggctg accgccctca
     1681 agatggatgt ttcgtggatc gccgcgcgcc tgcccaagga actgccgcgc ctcatggaca
     1741 agaccgccaa gatgaaagag gtcgtcgata ccacggtcgc cgctgtgcgc cgcatcgcct
     1801 cggacttgag gccggtgatg atcgacgatc tcgggctcat tccggcgatc gagcatctgc
     1861 tgcatgattt ttcgcagcgc tccggcgtcg tggtgagcct ggacgcgcgt accgatacgg
     1921 tagatctcgc cgacccgata gcgaccgcag tctatcgcat ggtgcaggag gcgctcacca
     1981 atgtcgcgcg gcacgccgag gcgacacgcg tcgaagtgag cttcgcgatc gaaggcgaga
     2041 acctggtggc gaaggtttgc gacaacggcc ggggcatcga cgaggcggcg atgcgcaagg
     2101 gcaagtcgta tggcgtactc ggcatacggg agcgggcgca gacgctcggc ggcagcgcgc
     2161 gcatctacaa gcccgatggg ggcgggacca ccgtggagat caccattccg ctccgggccc
     2221 aggcgcgcat ggagtccgtc gcatgatacg cgtcgtgctc gccgatgatc accgcatggt
     2281 tcgcaccggg ctcaaggaga tcctcgccga taccggcgac atcgaggtgg tgggcgaggc
     2341 gacgaacggg cacgaggtca tggcgcgcgt gcgcgagctt ctcttcgacc ttctggtgct
     2401 cgacatgtcg atgcccggcc gctccggaat cgatctgatc cgtcaggtaa agagcgaaaa
     2461 gccggcgctg cgcattctgg tcttgagcat gcacagcgaa gagcagtacg cagtgcgcgc
     2521 gctcaaggcc ggcgccgcgg gctatctaac caaggagagt gcggccgacg aactggtggc
     2581 cgcgatccgc cgcatcgcgg ggggcggcgc ttacgtgtcg ccggaaactg cccagcgcct
     2641 tgcgctcggt ctcgaggcag ggcgcgagcg cccgccgcac gagtcgctgt cggaccgcga
     2701 aatgcaggtc tttcacatga tcgcggcagg aaaatccgtg accgacatcg ccgatacgct
     2761 cacgctgagc gtaaagacgg tcagcacgca taaaacgcgc atcatgcaga agctcaacgc
     2821 gcacaaccag acggacctca ttcgctacgc catccggcac aagctggtgg acgatccggc
     2881 cgagtcggaa taggcgcaaa gccagcgggc cgcacgccga gcctagaaca gcatgtacca
     2941 gaggaagatc aggaagagga tcgtcgcagc cccgatcccg taaaggataa agcg
//
LOCUS       DASURG010000472         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1103385, whole genome shotgun sequence.
ACCESSION   DASURG010000472 DASURG010000000
VERSION     DASURG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103385"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFY80_08335"
     CDS             <1..489
                     /locus_tag="VFY80_08335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6007957.1"
                     /translation="GAHIHVVGGETPGEEALFQEFVQGATATEHLTYHGRVPYRAACA
                     LYGRAHVLLNTSDVEGFPNAYLQAWQNGVPVVTLIDPDGVIEREGLGIVCSSAEELFG
                     AVQRLLGDQTAWAHASTRCSAFMARHYNEDRILADYLAAFRDACTTTCEQRTLSPVSR
                     HV"
     gene            482..1519
                     /locus_tag="VFY80_08340"
     CDS             482..1519
                     /locus_tag="VFY80_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HEX6007958.1"
                     /translation="MSDTPFIITIDTEGDNLWARPHEITTRNAAYLPRFQALCERYRF
                     KPVYLTNYEMALSDEFVEFGRSVVSREAGEIGMHLHAWNSPPLHALTTDDYRHQPYLI
                     EYPAPIMKQKIRRLTALLEDRFERRMVSHRAGRWAFDGRYAAMLLDAGYRVDCSVTPG
                     VDWRPNKGAPQGNGGSNYTRFPDRPYFMSPTDISVPAKSGLLEVPMTVRSGRLYRRIP
                     FAYRVPCVRWIAKHISPAPRWLCPTSLQEKHNLHPMVELARQARTDGASHLEFLLHSS
                     ELMPGGSPAFRTPQDIDRMYEHLEILFDELSAWCSGTTLAELDGMLRRAAGTLAAKPP
                     VHDPHQAPIPA"
     gene            1516..2376
                     /locus_tag="VFY80_08345"
     CDS             1516..2376
                     /locus_tag="VFY80_08345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6007959.1"
                     /translation="MSSARGAVIHDWIKKAIMIRYRGRATTYGEVWFDEGLPEPCDVD
                     VVFCRQRATPVPGARTTSTLSLVVDLSRTEGELMDAFGKDCRYKVKRAESRDELELEF
                     IDEPAGRLEEFRAFYDGFAAEKGLRPCYRDWLAAACAARQLVLTCATHHGEPLVWHAL
                     LVAGGNAWLQYTGSCYRNRDNAYRALVGRANRWLHWQEMLRFRSRGLPRYDWGGLFED
                     ESTPERAGINRFKKEFGGTAVRRYDCVLPITLKGRMWLPLRDAWQHRPALRALSAALL
                     QRRLSSARAG"
     gene            complement(2389..2868)
                     /locus_tag="VFY80_08350"
     CDS             complement(2389..2868)
                     /locus_tag="VFY80_08350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6007960.1"
                     /translation="MDAKTRKKALRLLSHGVYVLTSRSAERYGAATITWVSQASFKPP
                     LLMAAIRRDSNVFKCLEQSGTAVLHIVGDGQQEIARRFFFPTDAAGETINGEPFGEGA
                     TTGPVLMRLPAHVECAVDRILDTGGDHAVVILRVVDAACREQVRPLTIAETSWNYGG"
     gene            complement(2888..>3190)
                     /locus_tag="VFY80_08355"
     CDS             complement(2888..>3190)
                     /locus_tag="VFY80_08355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6007961.1"
                     /translation="KIMEYMALGKPIVQFDLTEGRYSAQDASLYARRNDVRDFAAKIL
                     ELLDDPERRARMGEFGRRRIENELEWRHEFPKLIAAYEALWRAPDAATAARGIGAR"
BASE COUNT          561 a         1057 c         1000 g          572 t
ORIGIN      
        1 ggcgcacaca tccatgtcgt gggaggagaa acccctggcg aagaggcact gttccaggag
       61 ttcgttcaag gtgcaacggc gaccgaacat cttacctatc atggtcgcgt gccctacaga
      121 gcggcgtgtg cgctgtacgg ccgggcacac gtgctgctca acacttccga tgtggaagga
      181 tttcccaacg cctacctgca ggcatggcag aacggcgtgc cggtcgtcac gcttattgac
      241 cccgacggtg tgatcgagcg cgaaggtctc ggtatcgtct gctcctctgc cgaagaactt
      301 ttcggtgccg tccagcgttt gctgggcgat cagacggcct gggcacacgc cagcacgcgc
      361 tgcagcgcct tcatggcccg ccactacaat gaagaccgaa ttttggcgga ctatctcgcg
      421 gcgtttcgcg atgcttgtac gactacgtgc gaacagcgaa cgctctctcc ggtgagccgc
      481 catgtctgac acaccgttca tcatcacgat cgacaccgaa ggtgacaacc tgtgggcgcg
      541 cccgcacgaa atcaccacgc gcaatgccgc ctacctgccg cgctttcagg ccctgtgcga
      601 gcgctaccgc ttcaagcccg tctatctgac caactacgaa atggcgctct cggatgaatt
      661 cgtcgagttc ggtcgctcgg tcgtcagccg cgaggcgggc gagatcggga tgcatctgca
      721 cgcctggaat tcgccgccgc tgcacgcgct caccacggac gactatcgcc accagccgta
      781 tcttatcgag tatcccgccc caatcatgaa gcagaagatc cgcaggctca cggcgctgct
      841 cgaggatcgc ttcgagcggc ggatggtgag ccatcgcgcc ggccgctggg cgttcgacgg
      901 ccgctacgcg gcgatgctgc tcgatgcggg ctatcgcgtc gattgctcgg ttacgcccgg
      961 cgtcgactgg cgcccgaaca aaggggcacc gcagggcaac ggcggctcga actacacgcg
     1021 cttcccggac cgaccctatt tcatgtcgcc aaccgacatc tcggtgcccg caaaaagcgg
     1081 gctgctcgaa gtgcccatga cggttcgctc cggtcggttg tatcgccgca ttccgtttgc
     1141 ctatcgtgtt ccgtgcgtgc gatggatcgc caaacacatt tcaccggcgc cgcgctggct
     1201 ctgcccgacg tcgctgcagg agaagcacaa cctgcatccc atggtcgagc tcgcccgaca
     1261 ggcgcgcacc gacggcgcaa gtcatctgga gttcttgctg cactcgtcgg agcttatgcc
     1321 aggcggcagc ccggcgttcc gcacgcctca agatatcgac cgcatgtacg agcatctcga
     1381 aatactgttc gacgagctct ccgcgtggtg tagcggcaca acgctggcgg aactcgacgg
     1441 catgttgcgc cgggcagcgg gaacgcttgc tgcaaagccg cccgtgcacg atccgcacca
     1501 ggcgcccatc cccgcatgag cagcgcgcgg ggcgcggtca ttcacgactg gataaagaaa
     1561 gcgatcatga tccgttacag aggtcgcgcc accacctatg gcgaagtgtg gttcgacgaa
     1621 ggtctgcccg agccgtgcga cgtcgatgtc gttttttgcc gccagcgcgc cacgccggtt
     1681 cccggtgccc gaaccacatc aacgctttcg ctggtcgtcg atctctcgcg aaccgagggc
     1741 gaactgatgg atgccttcgg caaggactgc cgttacaagg taaaacgggc ggagtcgcga
     1801 gacgagctcg agctcgagtt catcgacgag ccggcgggcc ggctcgagga gttccgtgcg
     1861 ttttacgacg gctttgccgc ggagaaaggg ctgcgcccgt gctaccgcga ctggctggcg
     1921 gccgcctgcg ctgcgcgcca gctcgtcctc acatgcgcca cgcaccacgg tgagccgctg
     1981 gtctggcacg cgctgctcgt cgcgggtggc aatgcatggc tgcaatacac gggatcctgc
     2041 taccgcaatc gcgataatgc ataccgggcg ctggtcgggc gggccaaccg ctggctgcac
     2101 tggcaggaaa tgctgcgctt tcggtcgcgc gggctcccgc gctatgactg gggcgggctc
     2161 ttcgaggacg aatcgacgcc cgaacgtgcc ggcatcaacc gcttcaagaa ggaatttggc
     2221 ggaacggcgg tgcggcggta cgactgcgta ctgccgatta cgctgaaggg ccggatgtgg
     2281 ctgccgctgc gcgacgcgtg gcagcatcgc ccggccttgc gggcgctgag cgcggcgctt
     2341 ctgcagcgga ggctctcgag cgcgcgggcg ggttgacgcg ctagacagtc acccgccgta
     2401 attccaggac gtctctgcga tggtcagcgg gcgcacctgc tcgcggcacg ccgcatccac
     2461 cacgcgcagg atcaccactg catgatcgcc tccggtatcg agaatgcggt ccaccgcaca
     2521 ttcgacgtgc gccggaagcc gcatcagtac cgggccggtg gttgcgccct caccgaacgg
     2581 ctcaccattg atcgtttctc ctgccgcgtc cgtcgggaag aaaaagcgcc gcgcaatctc
     2641 ctgttggccg tcgcccacga tgtgcagcac cgccgtgccg ctttgttcca ggcacttgaa
     2701 gacgttgctg tcgcgccgga tcgccgccat gaggagcggg ggcttgaacg acgcctgcga
     2761 aacccacgtg atcgtcgcag cgccgtagcg ctcggcgctg cgcgaggtaa gcacatagac
     2821 gccatgcgag agcaggcgca gcgctttctt gcgggttttt gcatccatca tccggcaacc
     2881 cggaccgcta tcgtgccccg atgccccggg ccgcggtcgc ggcatcaggc gcgcgccaca
     2941 acgcctcgta ggccgcgatc agtttcggaa actcatgacg ccactcgagc tcgttctcga
     3001 tgcgacgccg gccgaattcc cccatccgcg cgcggcgttc cgggtcatcg agcagttcaa
     3061 ggatcttggc cgcgaagtcg cgcacatcgt tgcggcgcgc gtagagcgaa gcgtcctgcg
     3121 ccgagtaacg gccctcagtg agatcgaact gcacgatcgg ctttccgagc gccatgtact
     3181 ccatgatctt
//
LOCUS       DASURG010000473         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_82447, whole genome shotgun sequence.
ACCESSION   DASURG010000473 DASURG010000000
VERSION     DASURG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82447"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFY80_08360"
     CDS             <1..798
                     /locus_tag="VFY80_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007962.1"
                     /translation="PYDVSTPVAGDWYVSAEIPPSPVAAHLQNLELGEVAPLQAVFTA
                     ASAAGWTPLYLGRTRRGGHAPILQALQIDNRRTALVLGSGYWRWAFRGGAGRDLYYRL
                     WGSLAGWMVQDQPAIAGAAVRPVDRSVPRGEALRWLTPGMTPDSVEIRIAAGGSVITR
                     VVSRAQQGDTTITAPVAPGHYRYQARAFEDGAVAAESEGAITVESYSPEFMRAPADLG
                     LLRSAPTSLAEQTRRGRRPLHASPWPYGLLVLLLCAEWVLRRRWGLR"
     gene            765..1733
                     /gene="ftsY"
                     /locus_tag="VFY80_08365"
     CDS             765..1733
                     /gene="ftsY"
                     /locus_tag="VFY80_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX6007963.1"
                     /translation="MGAAPPLGLEIEGTNCNVRLKSTEKKSLWQKIKDVALTDVGVLV
                     RGMDEGSLEKLEELLIASDFGVNATMRLVGTVESLSRQGKIRTEEQFRQTVEQDIVEI
                     LQTGETRSELNFSQDGGPTVILMVGVNGVGKTTSIGKLAYKLKREGHRVLLAAGDTFR
                     AGAIDQLRIWSERVGAEFVGAQPGGDPAAVAFDAIDAAQARNCDVIIIDSAGRLHTQS
                     DLMLEIAKVERVIARKLPGAPHETLLVLDATVGQNAVAQARTFSQALKLSGLVLAKLD
                     SSARGGVVVALKQEFGLPVKLVGTGEQLGDLEAFEPESFAQEVLSV"
     gene            1733..>2397
                     /locus_tag="VFY80_08370"
     CDS             1733..>2397
                     /locus_tag="VFY80_08370"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX6007964.1"
                     /translation="MNARPRQASPLDAPAQFLKGVGPKRAELLARLDIHTARDLLYHT
                     PRRYEDASTVTAIEKAEIGLDVTIIGEVVSKGVIPTRAGLRIFQAVLKDGTGRIEVSW
                     PGQPFLDRSIHKGDILLVTGSVRFFHGRVIQPREFIVLGNVEEQTGNKGVVLPIYPAT
                     EGLSQRMLRSIVDANVPQLLGSAQVDEVFDARILKEIGLPPLQTALQWVHQPETLEQA
                     NRGR"
BASE COUNT          434 a          718 c          820 g          425 t
ORIGIN      
        1 ccgtacgacg tcagcacacc ggttgccggt gactggtacg tgtccgcgga gataccgccg
       61 tcgccggttg ccgcgcattt gcagaacctg gaattgggtg aagtggcgcc gctgcaagcg
      121 gtgtttaccg cagcgtcggc agcaggctgg acgccgcttt atctggggcg cacgcgccgg
      181 ggtggccatg cgcccatcct ccaggcgctg cagattgaca atcgccggac cgcacttgtc
      241 ctggggagcg ggtattggcg ctgggccttt cgcggcggtg ccggtcgtga cctctattac
      301 cgactgtggg gctcgttggc cggctggatg gtacaggacc agcctgcgat cgcgggtgcg
      361 gccgtgcggc cggtggacag gagcgtgccg cgtggcgagg cgctgcgctg gctgacgccg
      421 ggcatgactc ccgactcggt ggagatccgg atcgccgccg gcggcagtgt gatcacacgc
      481 gtggtgagtc gcgcgcagca gggggacacc acgatcaccg cgccggtcgc gcccgggcat
      541 tatcgctacc aggcgcgcgc tttcgaggac ggtgccgtcg cggccgagtc ggagggtgcc
      601 attacggtcg agtcgtacag cccggagttc atgcgcgcac cggcggatct ggggctattg
      661 cggagtgcac caacatcact ggccgaacaa acgcgtcgcg ggaggcggcc attacatgcc
      721 tcgccctggc cctatgggtt actggtcttg ctattgtgtg cggagtgggt gctgcgccgc
      781 cgttggggct tgagatagaa ggaacgaatt gtaacgtgag actgaagagt accgaaaaga
      841 aatcactctg gcagaagatc aaggatgtag cgctcaccga tgtcggtgtg ctcgtgcgcg
      901 gcatggatga gggttcgctg gaaaagctgg aagagctgtt gatcgcgagc gatttcggcg
      961 tgaatgccac catgcgcctg gtcggcaccg tcgagtcgct gtcgcggcag ggaaagattc
     1021 gtacggaaga acaattccgt cagaccgtcg agcaggatat cgtcgaaatt ctgcaaaccg
     1081 gggaaacgcg cagtgagctg aacttcagcc aggacggcgg accgacggtc atcctgatgg
     1141 tgggtgtcaa cggcgtcggc aagacgacca gcattggcaa gctcgcgtac aagctgaagc
     1201 gtgagggaca cagggtcctg ctggccgcgg gcgacacgtt tcgcgccggc gcgatcgatc
     1261 agttgcgcat ctggtccgaa cgggtgggtg cagagttcgt cggtgcgcaa cctggcggtg
     1321 acccggccgc cgttgccttc gacgccatcg acgctgcgca ggctcgcaat tgcgacgtca
     1381 tcattatcga cagtgccgga cgcctgcaca cgcaatccga tctcatgctg gagattgcga
     1441 aagtcgaacg cgtgattgcc cgcaagctgc cgggcgcgcc gcacgaaacg ctgctcgtgc
     1501 tggacgccac cgtcggccag aacgcggtgg cgcaggcacg cacgttcagc caggcactga
     1561 agctcagcgg cctggtgctg gccaagctgg acagcagtgc caggggcggt gttgtggttg
     1621 ccctcaaaca ggagttcggt ttgccggtca agctcgtggg caccggtgag cagctgggcg
     1681 atctcgaagc attcgagccg gaaagctttg cgcaggaggt tctgtccgtc tgatgaatgc
     1741 gcgcccgcgc caggcttcgc cgctcgatgc acccgcgcag tttctcaagg gcgtgggacc
     1801 gaagcgtgcc gagctgctcg cgcgcctcga catacacacc gcgcgcgacc tgctctatca
     1861 cacaccgcgc cgctacgaag atgccagcac ggtcaccgct atcgagaagg cggagatcgg
     1921 cctggatgtc accatcattg gtgaagtcgt cagcaaaggc gtcattccca cgcgcgccgg
     1981 cctgcgcatc ttccaggcgg tcctgaaaga cggcaccggc cgcatcgaag tttcctggcc
     2041 gggccagccg tttctcgatc gcagcattca caagggcgac atcctgctgg tcacgggcag
     2101 tgtccgcttc tttcacggac gcgtcatcca gccacgcgag ttcatcgtac tcggcaacgt
     2161 cgaagaacag accgggaaca agggtgtggt gctgcccatc tacccggcca cggaaggcct
     2221 gtcgcagcgc atgctgcgca gtatcgtgga tgccaacgtt ccgcaattgc tcggctctgc
     2281 acaggtcgat gaagtattcg atgctcggat cctgaaggag atcgggctgc cgccactgca
     2341 gacggccctg cagtgggtgc atcagcccga gacactcgag caggcgaatc gcgggcg
//
LOCUS       DASURG010000474         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1514094, whole genome shotgun sequence.
ACCESSION   DASURG010000474 DASURG010000000
VERSION     DASURG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514094"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2279)
                     /locus_tag="VFY80_08375"
     CDS             complement(<1..2279)
                     /locus_tag="VFY80_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6007965.1"
                     /translation="MSMKDATRAPARRLLEKAAHGVILLGEAITDCNDAASRALELER
                     EALIGQSLIDLSPRIQADGTLSSERWTRRVQAAKAGLPQWFQWQFRRAGAEIVHALVH
                     LSPASDDDGSEVIAEVHDLSRLSGAGWVHKESEARLRDVLQHTKPLIFVKDRDGRYIF
                     VNHELERVVRRPAQEIIGRTDHELWRSELAERFRRNDLQVLDERQAIEFNVTDTFGEQ
                     QRTFLSFKFPLFDADGAPYAVCGIATDISERKQLEDALRGAALAVSSAQGATVFQELV
                     RYLAATLKLDCALIAIPIDGNTACNMRVLAFVLDGEIRENFEYCIAGTPCETVLGKSF
                     RVYPSHLTAAFPNDVDFSRLGFESYAGYPLNDSTGNSLGLIAVVSRKPMADPGFIESV
                     LKIFAVRAAAELERLRAEEALRTSEASYRAIFEASEDAIFVHDWDTGAIVDVNPTACA
                     AYGYTAEEMKRVSIADLSSGVHPYTMEEAAKLHERAKAGGPLRFEWHRRNKDRSLHWD
                     EVVLKKAVIAGKPRVVAFTREITERKLAEQALRASEEHYRAIFNASSEGLVLRDADFR
                     VVDVNPAFLAMTGYSREEALGARHVLTMPVADLERARELQRRALAGETVHIEGNGLRR
                     DGTQYDCEVVLASMQFRGKPHVLGIVRDISARKQAEARREQLEAQLRQAQKMEAIGQL
                     TGGIAHDFNNLLTSIMGYIVLATERDTVHGDAKLAKYLEQAHLSCGRARDLIQQMLTF
                     SRGQRGEPRPLSLAPCVKES"
     gene            complement(2371..>2711)
                     /locus_tag="VFY80_08380"
     CDS             complement(2371..>2711)
                     /locus_tag="VFY80_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6007966.1"
                     /translation="ATPPSGQGQQRIAVGRCQLDLASHQLFDGVGEEVPITTMEYELL
                     KTFVEHPNKVLTRDQILTLTRNREWEPFDRSIDIRIARLRRKIEVDPDKPQAIRTVRG
                     AGYMFVPARA"
BASE COUNT          446 a          878 c          903 g          484 t
ORIGIN      
        1 gactccttca cgcacggtgc gagcgacagc ggccgcggct cgccgcgctg tccgcggctg
       61 aaggtcagca tctgctggat cagatcgcgc gcgcgcccgc aggataggtg cgcctgctcg
      121 agatacttgg cgagcttggc atcgccgtgc accgtgtcgc gctccgtcgc gagcacgatg
      181 taacccatga tgctcgtcag cagattgttg aagtcgtgcg cgatgccgcc cgtcaactgc
      241 ccaatcgcct ccattttctg cgcctgtcgc aactgcgcct cgagttgctc gcgacgcgcc
      301 tccgcctgct tgcgcgcact gatgtcgcgg acgatgccga gcacgtgtgg cttgccgcgg
      361 aactgcatgg aagcgagcac gacctcgcaa tcgtactgcg tgccgtcgcg gcgcaggcca
      421 ttgccctcga tatgcacggt ctcgccggcg agagcgcggc gctggagctc gcgcgcccgt
      481 tcgagatcgg cgacgggcat ggtgagtaca tggcgcgctc cgagcgcctc ctcgcggctg
      541 tagccggtca tcgcgagaaa cgccggattc acgtccacca cgcggaaatc ggcatcgcgc
      601 agcacgaggc cttccgacga tgcattgaag atcgcccggt agtgctcctc gctcgcgcgg
      661 agcgcctgct cggcgagctt gcgctcggtg atctcgcggg tgaaggcgac gacgcgcggc
      721 ttgcccgcga tcaccgcctt cttgagcacc acttcgtccc aatggaggct acggtccttg
      781 ttgcgccgat gccattcgaa ccggagcggc cccccggcct tggcgcgctc gtggagcttc
      841 gccgcttcct ccatggtgta ggggtgcacg ccggaactca agtctgcgat gctgacccgc
      901 ttcatttcct cggcggtgta gccgtacgcc gcgcacgccg tggggttcac gtccacgatc
      961 gccccggtat cccagtcgtg cacgaagatc gcgtcttcgc tcgcttcgaa gatcgcgcgg
     1021 tagctcgctt ccgaggtgcg cagcgcttcc tcggcacgca gtctttcgag ctccgcagcc
     1081 gcgcgaacgg cgaagatctt gaggaccgat tcgatgaaac cgggatcggc catcggtttg
     1141 cgcgacacga cggcgatcag cccgagcgaa ttgccggtgg agtcgttcag cggataaccg
     1201 gcgtagcttt cgaaacccag acggctgaaa tcgacgtcgt tcgggaaggc ggccgtgagg
     1261 tgcgaaggat agactctgaa ggacttgccc agcaccgttt cgcagggcgt gcccgcaatg
     1321 cagtactcga agttctcgcg aatctcgccg tcgagcacga acgcgagcac gcgcatgttg
     1381 cacgccgtat tgccgtcgat cgggatcgcg ataagcgcgc aatccagctt gagcgtcgcg
     1441 gcgaggtagc gcaccagctc ctggaacacg gtcgcgccct gtgcgctcga taccgcgagc
     1501 gcggcgccgc gcagggcgtc ttccagctgc ttgcgctccg agatgtcggt cgcgatgccg
     1561 cacaccgcgt agggcgcgcc gtcggcatcg aagaggggaa acttgaacga gagaaaagtg
     1621 cgctgctgct cgccaaaggt gtccgtcacg ttgaactcga tcgcctggcg ctcatcgagc
     1681 acctgcagat cgttgcgccg aaagcgctcg gcgagctcgc tgcgccagag ttcgtgatcg
     1741 gtgcggccga tgatttcctg cgcggggcgg cgtacgacgc gttcgagctc gtgattgacg
     1801 aagatgtagc gcccgtcccg gtccttgacg aagatcagcg gcttcgtgtg ctggagaaca
     1861 tcgcgcagcc gcgcttcgct ctccttgtgg acccagccgg cgccggaaag gcgcgacaag
     1921 tcgtgcactt cggcgatgac ctccgagccg tcgtcgtcgc ttgcgggact caagtgcacc
     1981 agagcgtgga cgatctctgc gccggcgcgc cggaactgcc actggaacca ctgcggcaga
     2041 ccggccttgg cggcctgaac tcgccgcgtc cagcgttccg acgaaagcgt tccatccgcc
     2101 tggatgcggg gcgagagatc gatcagggac tggccgatca gcgcctcacg ctccagttcg
     2161 agggcgcggg aggcggcatc gttgcaatcc gtgatcgcct caccgagcag gatcaccccg
     2221 tgcgcggcct tttccagcag gcggcgcgca ggggcgcggg tcgcgtcctt cattgacatc
     2281 agccgttagt cgtctcgcgg ggagtatagg cacgaaccgc gccataccgc gcgactgtta
     2341 cacggcagaa acaattgaaa cgaaaggaga tcaggcgcgc gccgggacga acatgtagcc
     2401 cgccccgcgc accgtgcgga tcgcctgcgg cttgtcggga tcgacctcga tcttgcgacg
     2461 cagcctggcg atgcggatgt cgatggagcg gtcgaagggc tcccactcgc gattgcgggt
     2521 gagggtgagg atctggtcgc gggtcagcac cttgttgggg tgctcgacga aagtcttcag
     2581 gagctcgtat tccatggtcg tgatgggtac ttcctcacct acgccgtcga agagctgatg
     2641 tgacgcgagg tcgagctggc agcggcccac ggcgatgcgc tgctgcccct gtcccgaggg
     2701 cggcgtcgca g
//
LOCUS       DASURG010000475         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1103695, whole genome shotgun sequence.
ACCESSION   DASURG010000475 DASURG010000000
VERSION     DASURG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103695"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFY80_08385"
     CDS             complement(<1..293)
                     /locus_tag="VFY80_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6007967.1"
                     /translation="MDPVLCSVDDGVAVVTLNNPPLNLVTLELTRQLNALVTKLAMDP
                     TVRVMVLTGSGSKAFCAGSDIKEFPEMMAAGAVVPKKLALENEAYSRVDDFPKP"
     gene            complement(491..1663)
                     /locus_tag="VFY80_08390"
     CDS             complement(491..1663)
                     /locus_tag="VFY80_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6007968.1"
                     /translation="MANDTSRAISAIYDAVLSDEHWPAALDAFARHIGSVGAVLVAVD
                     KVGLPFTILQATSNYPIEDVHYYFNNLGQYDEPVMTSTFAVTPRFQLLRDEDVWGDIS
                     KLENRPDYKWVRERIGANRRAGVRLSGEKGWMDLVALQFAERDWRLVPETMPARLARL
                     VPHLAKAVEVNRTFTLLREAYHAVLSVLDFVRIGIAIVGPGGLVITYNNELRRIFELR
                     DGLRLSPNGRLECTSVAQGAELASAIAEATSTSAGDGETTERLLFIERRSRRRPFLIE
                     VAPLRDITGDLQRGFAGAVVFVIDPENARAISTDRLARLFQLSPAEAAVVRAMVDGLS
                     AGEIADLRGTKEDTVRSQFKAIYAKTGVRRRADLVRLAVSVDPPIDRSGSTTTGAN"
     gene            complement(1768..>2388)
                     /locus_tag="VFY80_08395"
     CDS             complement(1768..>2388)
                     /locus_tag="VFY80_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6007969.1"
                     /translation="TITGMTKAMHAKPRSPGDYRTCGVPRTGVLVRVVDDQDRDLPVG
                     DVGEVITRSDCVMEGYWNNPTATEETLRGGWLHTGDIGSMDADGYLTLRDRSKDLIIS
                     GGSNIYPREIEEVLLRHPDLIEASVVGRPHPEWGEEVIAFVVARRGARVETAALDALC
                     LENIARFKRPRAYRFVESLPKNNYGKVLKTELRAILQKEKETGRGT"
BASE COUNT          475 a          716 c          708 g          489 t
ORIGIN      
        1 ggtttcggga aatcgtcaac gcggctgtag gcttcgttct ccaaggcaag cttcttcggc
       61 acaacggcgc ccgctgccat catttccgga aattccttga tgtcggagcc agcgcagaat
      121 gcctttgagc ccgatccggt cagcaccatc acgcgaaccg tagggtccat tgcgagcttc
      181 gtgaccaatg cattgagctg tcgggtcaat tcgagcgtga cgagattgag cggtggatta
      241 ttgagtgtga cgacggcaac gccgtcgtcc acggagcaga gcaccggatc catcctgagc
      301 gtcctttccc ctcgttgtcg aatgatgtgg ggacgatcat gacccagccg cagggaattt
      361 gaaagcgctt acctgaagtg cctgggtgca agtgagctgt gcaaagttga tgaagctcat
      421 ccggcttata tctcgagcgg agccgctcat ctgcccgatt tatcggctct cggcgtgctc
      481 ggattcgagc ctaattcgcc cccgtcgtgg ttgaacccga ccgatcgatc ggaggatcga
      541 cactgacggc aagacggaca agatcggctc ggcggcggac gcctgttttc gcgtagatgg
      601 ccttgaactg cgagcgcacg gtgtcttcct tcgtaccgcg caagtcggcg atctctcccg
      661 cggacagacc atcgaccatg gcgcggacaa cggctgcctc ggctggagaa agttggaaca
      721 gccgagccaa tcggtcggta gaaatcgctc tcgcattttc ggggtcgatg acgaacacca
      781 cagcgcctgc gaaaccccgc tgcagatcgc ctgtgatatc gcggagcgga gcgacttcga
      841 tcaggaaggg tcgcctgcgg cttctccgct caatgaaaag caggcgctct gtggtttcgc
      901 catctcccgc ggaagttgat gtcgcctcgg cgatcgcgga agcgagctct gcgccctgcg
      961 ctacgctggt gcattcgagc cggccgttgg gagagaggcg gagcccgtct ctaagctcaa
     1021 aaatgcgccg cagctcattg ttgtacgtga tgaccaatcc accgggtccg acaattgcaa
     1081 tgccgatgcg aacaaaatcc aggacggaca gcacggcatg gtaggcctca cgcagcagag
     1141 tgaatgtgcg attgacctcg accgcttttg cgagatgggg caccagccgc gctaagcgag
     1201 cgggcatagt ttccggcacc aggcgccagt ctctctctgc gaactgcagg gcgacgagat
     1261 ccatccagcc tttttcgccg gaaaggcgga cacccgcacg ccggttcgca cctatccgct
     1321 ctcgaaccca tttgtaatct ggccgattct cgagcttcga aatatcgccc cagacgtcct
     1381 cgtcgcgcaa aagttggaag cgcggcgtaa cagcgaacgt actcgtcata acgggctcgt
     1441 cgtactggcc caaattgttg aaataataat ggacgtcctc gatcgggtaa ttgctggttg
     1501 cctgtaggat cgtgaagggc aggccgacct tgtcgacggc tacgagcacg gcgccaaccg
     1561 atccgatatg ccgcgcaaac gcatcgagcg cggccggcca atgctcgtct gagaggaccg
     1621 cgtcgtagat cgccgatata gctcgactgg tgtcgttggc catattcata ccccaaaaac
     1681 caagtgtagg agggtatacg gatcaaacga gcacgccaag cgtggaggtg ctgatcttgg
     1741 tgaacgttcg ctaccttgtt gcgccagtca ggtgccgcgc cctgtctcct tctccttctg
     1801 gagtatggcc ctcagctcgg tcttgagaac tttaccatag ttgttcttgg gcaaactctc
     1861 gacgaaccga taggctctgg ggcgtttgaa acgggcgatg ttctcgagac agagggcgtc
     1921 cagggctgcg gtttcgaccc tggcaccgcg tcgcgcgacg acgaaggcga tgacctcttc
     1981 gccccattcc ggatgtggtc ggccgaccac cgaagcctcg atcaaatcgg gatggcggag
     2041 cagcacttcc tcgatctcgc gtggatagat attactcccg ccactgatga tcaggtcctt
     2101 cgaacggtcc cgcagcgtga ggtagccatc agcatccatg ctgccgatgt cgcccgtgtg
     2161 cagccacccg cctctgaggg tttcttcggt ggccgtcgga ttattccagt aaccctccat
     2221 cacgcagtcg ctccgcgtaa tgacctcacc cacgtcgccc accggcaggt cgcgatcttg
     2281 atcgtcgacg acgcgcacca agacgcccgt gcgcggcacg ccacaggtgc ggtagtcccc
     2341 cggagagcga ggtttcgcat gcatcgcctt ggtcatgccg gtgatggt
//
LOCUS       DASURG010000476         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1103741, whole genome shotgun sequence.
ACCESSION   DASURG010000476 DASURG010000000
VERSION     DASURG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103741"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..856)
                     /locus_tag="VFY80_08400"
     CDS             complement(80..856)
                     /locus_tag="VFY80_08400"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX6007970.1"
                     /translation="MPKDISQAVREVCLWFPESSEVIAHGSPDFRVRGKTFATYVINH
                     HGDGRVALWLNAPAGAQELYTREEPKHFFVPPYVGPRGWLGVRLDQGISWKRVAKLVR
                     EAYEKVAPPALTAQLGKTIEIAPPTRMLSAAQIDPMQSKRAQSVLKSMRRICLALPET
                     SEDRQFGFPVWRAGKKTFAQAYQYRDTDKLRLAFWVGVERQAMLTGDERYTIPQYMGH
                     NGWIALDVSKHLDEDEVRELVLFSYRHFALKRMLQRLGEP"
     gene            934..1161
                     /locus_tag="VFY80_08405"
     CDS             934..1161
                     /locus_tag="VFY80_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX6007971.1"
                     /translation="MIRTVLTVVLALAAAAALAAESGAKPSFESLDKNSDGKVSLNEA
                     ADNDALFVAFKSLDTNKDGMLSREEFAAYKG"
     gene            complement(1254..1781)
                     /locus_tag="VFY80_08410"
     CDS             complement(1254..1781)
                     /locus_tag="VFY80_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007972.1"
                     /translation="MENRQILDYPPQFQKTVRMHTKVWIPPCAMALVLAACGQSQQSA
                     APAPAAPPFHTTLTTKQLMSWIIDPNATAIWNSVGSITTEKGTQEKHPRTDEEWSTYR
                     NAAAMLIESGNLLMLDGRAVDNDEWMTASRGMADAAATVLEAAEAKDVEAYFDAGGVL
                     YEACTACHSKYLVEP"
     gene            1876..>2123
                     /locus_tag="VFY80_08415"
     CDS             1876..>2123
                     /locus_tag="VFY80_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007973.1"
                     /translation="MRVISRALLVTAAAVFATSAMAAGAPAKGPRTKDGKPDLNGIWQ
                     ALNTANFDLEPHAAKAALAMVPGQFVPVPAPEIVKMGAV"
BASE COUNT          357 a          696 c          660 g          410 t
ORIGIN      
        1 tggtccgcct ggtccctgct gatcgcctac ggtttcgggc tgcatctgcc ggatgcaaca
       61 tccgggcgca gcgccagcgt cacggttcgc ccagccgctg cagcatgcgc ttcagcgcga
      121 agtgtcggta gctgaacagc acgagttctc tcacttcatc ctcgtccagg tgcttgctga
      181 cgtcgagcgc gatccagccg ttgtggccca tgtattgcgg gatggtgtag cgctcgtcgc
      241 cggtcagcat cgcctgccgc tcgacgccaa cccagaatgc gaggcgcagc ttgtccgtgt
      301 cgcgatactg ataggcctgc gcgaacgtct tcttgccggc acgccacacg ggaaagccga
      361 attgccggtc ctcggaagtt tccggcagcg cgagacagat gcggcgcatc gacttcagca
      421 ccgactgcgc gcgcttcgac tgcatcggat cgatctgcgc ggcgctgagc atcctggtcg
      481 gcggcgcgat ctcgatggtc ttgccgagct gcgcggtcag cgccggcggc gcgaccttct
      541 cgtacgcctc gcgcaccagc ttcgccacgc gcttccagga aataccctgg tccagccgca
      601 cgccgagcca gccgcgaggc ccgacatacg gcggcacgaa gaaatgcttg ggctcttccc
      661 tcgtgtacag ctcctgcgcg ccggccggcg cgttgagcca tagcgcgacg cgcccgtcgc
      721 catgatgatt gatcacgtac gtcgcgaagg tcttgccgcg cacgcggaaa tccggcgagc
      781 cgtgcgcgat cacctcgctg ctttccggga accacaggca tacttcgcgc accgcctggc
      841 tgatgtcctt gggcatgcgg cgagcataac cgcggggaac attcacgccc aaggcgctat
      901 tgtccggaca gtgcttgctg caggagatcc atcatgatcc gcaccgtgct aacagtcgtt
      961 ctggccctgg ccgccgctgc cgcgctggcc gccgagtcgg gcgcgaagcc gagtttcgag
     1021 tcgctggaca agaactccga cggcaaagtc agcctgaacg aggccgccga caacgacgcg
     1081 ctgttcgtgg cgttcaagag cctcgatacc aacaaggacg gcatgctgag ccgcgaggaa
     1141 ttcgccgctt acaagggatg acaaaccgcg aaggttgtgg ggtcgggctt tagcccgact
     1201 tctttttttg tcgggttgtt gttgtcgggc tgaagcccga cctgcagccc gacctacggc
     1261 tccaccaggt atttcgaatg gcaggcggtg cacgcttcgt acagcacacc gccggcgtcg
     1321 aagtaggctt cgacgtcctt cgcttccgcg gcttccagca cggttgcggc cgcgtccgcc
     1381 atcccgcgtg acgcggtcat ccattcatcg ttgtccaccg cgcgcccgtc gagcatcagc
     1441 agattgcccg attcgatgag catcgccgcg gcgttgcgat acgtggacca ttcctcgtcc
     1501 gtccgtggat gcttctcctg cgtgcccttc tcggtcgtga tgctgccgac ggaattccag
     1561 atcgccgtag cattcggatc gatgatccag ctcatgagct gtttcgtcgt caacgtcgtg
     1621 tgaaacggcg gcgcagccgg cgccggcgct gcgctttgct gcgattgtcc gcatgcagcc
     1681 agcaccagtg ccattgcgca gggtggaatc catactttgg tgtgcatgcg gacagtcttc
     1741 tggaattgcg gcggatagtc gagaatctga cgattctcca cacaattcaa cacgttcgcg
     1801 cctcagactg ggcgccgtat tgacaggcca tcgggcctca ttgttttatc gccgctcttg
     1861 ggggagacac aaacgatgcg agtcatttca cgcgcgctgc tggtcaccgc ggcggcagtt
     1921 ttcgcgacca gcgcaatggc cgcaggcgct ccagccaaag gcccgcgcac gaaggacggc
     1981 aagcccgacc tgaacggcat ctggcaggcg ctcaacaccg ctaatttcga cctcgagccg
     2041 catgccgcga aggcagcgct ggcgatggtg cccggccagt tcgtgccggt gcccgcgccg
     2101 gagatcgtga agatgggcgc ggt
//
LOCUS       DASURG010000477         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1411698, whole genome shotgun sequence.
ACCESSION   DASURG010000477 DASURG010000000
VERSION     DASURG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411698"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..553)
                     /locus_tag="VFY80_08420"
     CDS             complement(95..553)
                     /locus_tag="VFY80_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6007974.1"
                     /translation="MRIAMSWLGCALLLSAPVHAQSTREASTASEEQAVRVPLEHYLQ
                     GHATGDGAHFRAAFHPEARLFFIRDGRLSTRTSEEYIAGARGTPPADEAQRKRWIESV
                     DIAGNAAVAKIILDYPTTRFVDYMSLLKIEGAWKIVNKTFYAEPRSSGKP"
     gene            728..>2388
                     /locus_tag="VFY80_08425"
     CDS             728..>2388
                     /locus_tag="VFY80_08425"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6007975.1"
                     /translation="MSFVELRAHSAFSFGDGTASPEALIQRAAELGYRALGLTDTSDL
                     GGIIRFTLEAEKTGIKPICGAELKVDGFVTAFLACDQTGYRNLAHLVTKARVGSIGRW
                     RHEHGHEHEHGRVGTVTPKRGHNLAKRYAVPLPERGRPNLTFQDIVEHSEGLFCLTGP
                     QHGELGTLLSTDRKVEATYELDRWRDVFGHRLAVEVQLHHTGGNEAALATSLIELAER
                     NRVTWVASNDPRYLDANGRLVHDLLTANRADVCVNTAASWGLLLPNGEWRLKSPREIQ
                     TLWQGRAAGLAASCAIAEECQPFDLRWLRPPLPHFDVPAGHNDDSYLRELVRAGAAER
                     WGQVDERQRAQIEHELGVIQRLGFAGFFIVMWDAIRFARSRNILCQGRCSAANSAVAY
                     CLGITAVDPVKHGLLFERFLSEVRTDGRTEAPDIDVDFEMHRREEVLDYMYDRYQRQH
                     SAICAVTQFFHAPTAVQDMMRALGYPADMAFRISKRVHGSEPSEGAQLIEEGLGKEAG
                     LDSSDARGRALLSALRSLDDVPRLRSTHPGGFVLSCRPLGDYLPI"
BASE COUNT          405 a          707 c          790 g          486 t
ORIGIN      
        1 caacggcatc tgcggcagat cgagcgcatc aagagcatgg agggattttc gggcaatgcc
       61 ggcgcggcct tccgggtgta gcgaagcacg ctcttcacgg cttgcccgaa ctcctgggct
      121 ccgcatagaa cgtcttgttc acgattttcc acgcgccttc gatcttcagc agcgacatgt
      181 agtccacgaa acgcgtggtc ggataatcga gaatgatctt ggccaccgcc gcattgccgg
      241 cgatgtcgac actctcgatc cagcgcttgc gctgcgcttc atccgcgggc ggcgtgccgc
      301 gcgcgccggc aatatactct tcactcgtgc gcgtgctcag cctgccgtcg cgaatgaaga
      361 acagcctggc ttccggatgg aacgcggcgc gaaaatgcgc accgtcaccg gtggcatgcc
      421 cttgcagata atgctcgagc ggaacccgca cggcctgctc ttcggacgcc gtgctcgcct
      481 ctcgggtcga ctgggcatgg accggcgcac tcagcagcag cgcacaaccg agccatgaca
      541 ttgcgatacg catatggttc tccaggatgc aggttgatcg tatgtgatgc gtctgatcag
      601 gtttgagttg tccgggtcct gcgtctggga cggcaggcgc cggcgggtcg caggctgatg
      661 ggttctggca gaaggtgttt cgcgggtgtc ttgatcttcg gtaaatcttc gggtatcatc
      721 ccgaagtatg tcctttgtcg agttgcgcgc ccattccgct ttttcctttg gtgatggcac
      781 cgcctctcct gaagcgctca tccagcgggc ggccgagctg ggttaccgtg cgctgggtct
      841 gaccgatact tcggacctgg gcgggatcat ccgcttcacg ctggaagccg agaagacggg
      901 catcaagccg atttgtggtg cggagctgaa ggtcgacggc ttcgtcaccg cgtttctggc
      961 ctgcgatcaa accggctacc gcaacctcgc gcatctggtc acgaaagcgc gagtcggcag
     1021 catcgggcgc tggagacatg aacacggaca cgaacacgaa cacggtcgtg tcggtaccgt
     1081 gacaccgaag cgtggtcaca atttggccaa acgctatgcg gtgccgttgc ccgagcgtgg
     1141 cagaccgaat ctcacctttc aggacatcgt cgagcacagt gagggcctgt tctgcctgac
     1201 gggtccgcag catggtgagc tgggcacgct gctctcgacc gatcgcaagg tcgaagcaac
     1261 ctacgagctg gatcggtggc gcgatgtatt tggccatcgg ctggcggtgg aagtgcagct
     1321 gcatcacacg ggtggcaatg aagccgcgct ggcgacgtcg ctcattgagc tggccgagcg
     1381 caaccgcgtc acctgggtag cgagcaacga tcctcgttat ctcgatgcga atggccggct
     1441 cgtgcacgat ctgctcaccg ccaatcgcgc ggatgtgtgt gtgaacaccg cggcatcgtg
     1501 ggggctgctg ttgcccaatg gcgagtggcg gctcaagtca ccgcgtgaaa tccaaacctt
     1561 atggcagggg cgggcagccg gcctggctgc gagctgcgca atagcggagg agtgtcagcc
     1621 gttcgatctg cgttggctgc gcccgcccct gcctcatttc gatgtgccgg ccggccacaa
     1681 tgatgacagc tatctgcgcg agctggtgcg ggcgggcgca gccgagcgct ggggtcaggt
     1741 cgacgagcgg cagcgcgcgc agatcgagca cgagctgggt gtcattcagc gtctgggttt
     1801 tgccggcttc ttcatcgtca tgtgggatgc gattcgtttt gcgcgttccc gcaacattct
     1861 gtgtcagggg cggtgcagtg cggccaacag cgcggtcgcg tactgcctcg gcatcacggc
     1921 ggtcgatccg gtcaagcacg gcctgctgtt cgagcgattt ctttcggaag ttcggacgga
     1981 tgggcggacg gaagcgcccg atatcgacgt cgacttcgag atgcatcgac gggaggaagt
     2041 gctggactac atgtacgacc gctatcagcg ccagcactcg gcgatttgtg cggtgacgca
     2101 gttcttccac gcgcctactg cggtgcagga catgatgcgc gcgctgggtt accctgcgga
     2161 catggcgttc cgcatttcca agcgtgtgca cggcagcgag ccgagtgaag gcgcgcagct
     2221 catcgaggaa ggattgggta aggaggccgg tctcgactcg agtgatgcgc gcgggcgcgc
     2281 gttgctgtcc gcgctccgct ccctggatga cgtgccgcgc ttgcgttcta ctcatccggg
     2341 cgggttcgtg ctctcgtgcc ggccgctcgg tgattacctg cccatcga
//
LOCUS       DASURG010000478         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1206585, whole genome shotgun sequence.
ACCESSION   DASURG010000478 DASURG010000000
VERSION     DASURG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206585"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..2339)
                     /locus_tag="VFY80_08430"
     CDS             complement(147..2339)
                     /locus_tag="VFY80_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6007976.1"
                     /translation="MSVAPVRESQDQSVQVARLQAELAAARAEVESLQTLLKEQAVRS
                     RRIEESRQAWAQTVDALAQPMFMHDNKGCIVRANRAYAERAGVPVKNLIGRVYWKLFP
                     IQNAPFPVPESDAAPHEFELPLSEAEVFLVRSAGPSAGLPQGWRLYIFQDVTELKKAV
                     SAVRASGHYARSIVDSSLAAIVAVDRDRRILEFNPAAERAFGYTREEVIGRPANLLYA
                     DPESAEAIRRLVFERQGGVSEVENRRKNGEVFTSLMSAAILRDADGKALGILGTSIDV
                     TERKRAEAKMREVATELELLFENAVTGIAFTRELTIERVNRRFAELFGYPKEEFLGQT
                     LERVHAGKEDYERTLRDAEAHFNQNRVYRADVQLQRKDGSPVWIHVVARAVARERDAA
                     GAIWVFEDVTERKFAELRLERRETYFRALIENCSDVIAVIDAGAVIKYESRGIERLAG
                     YSSAERTGRSALELVHPDDAQRVRAAYQRIVQGEGQYAAIEFRMHRKDGSWRTVEGIG
                     SGAFDLDGEKVGVVNLHDVTERRHAEHRLVKGMEGAVAAIAAAAELRDPYTAGHERRV
                     ADLAAAIGREMGLPEERVRGLHLAGVVHDIGTIHVPAEILVKPSRLSALEQAMVRTHC
                     QAGHDVLKNIDFPWPIARIVLQHHELIDGSGYPLGLKGDEILLEARILSVADVVEAMV
                     SHRPYRPAVTIDAALSEIVMNRGTHYDARVVDACLKLFREKNYLLPAA"
BASE COUNT          433 a          845 c          787 g          433 t
ORIGIN      
        1 ggatgcgccg acgatcgcaa tggcgcgcgg attgaagaag cgttcgagat cggtgcgcat
       61 gtgtgcgacg ggcgcatgcg cggtgcacat gcggaaaagg cgacatgata tgcgcttccc
      121 gcgctcgcgc tcaaccgagc agggcgctat gcggcgggca ggaggtaatt cttctcgcgg
      181 aagagcttga ggcacgcatc gacgacgcgc gcgtcgtaat gcgtcccgcg gttcatgacg
      241 atctccgaaa gcgccgcatc gatcgtcacg gcgggtcggt agggacgatg cgagaccatg
      301 gcttcgacca catcggcgac gctcagaatg cgcgcttcaa ggaggatctc atcgcccttg
      361 agtcccagcg gataccccga gccgtcgatc aactcgtggt gctgcagcac gatacgcgcg
      421 atgggccatg gaaaatcgat gttcttcagc acgtcgtggc ccgcctggca gtgcgtacgt
      481 accatcgcct gctcgagcgc ggaaaggcgc gagggcttga ccaggatttc ggcgggcacg
      541 tggatcgttc cgatgtcgtg cacgacgccc gcgaggtgga gtccgcgcac gcgttcttcc
      601 ggcaacccca tctcgcggcc gatcgcggcc gcgagatcgg cgacacgccg ttcgtgcccc
      661 gcggtatagg gatcgcgcaa ttcagcggcg gccgcaatcg cggcaaccgc gccctccatg
      721 cctttcacga gccgatgctc ggcatgccgg cgttcggtga cgtcgtgcag gttcacaaca
      781 ccgacctttt cgccgtcgag atcgaaggcg ccgctgccga tgccctcgac ggtgcgccag
      841 ctgccgtcct tgcggtgcat tctgaactcg atcgccgcgt actgtccctc gccttgcacg
      901 atgcgttggt atgcggcgcg cacccgctgc gcgtcatcgg ggtggacgag ctcgagcgcc
      961 gagcgaccgg tgcgctccgc ggagctatag cctgccagcc gttcgatgcc gcggctttcg
     1021 tacttgatga cggctccggc atcgatgacg gcgatgacat cgctgcagtt ttcgatgagc
     1081 gcgcggaaat acgtctcgcg ccgctccagg cgcagctcgg cgaacttgcg ctcggtcaca
     1141 tcctcgaaaa cccagatcgc cccggccgca tcccgctcgc gggccaccgc tcgcgcgacg
     1201 acatgaatcc acacgggcga gccgtccttg cgctgaagct gcacgtccgc ccggtagacg
     1261 cggttctggt tgaagtgcgc ttccgcgtcc cgcagcgtac gctcgtagtc ctccttaccg
     1321 gcgtggacac gctccagcgt ctgaccgagg aattcctcct tgggatagcc gaaaagctcc
     1381 gcgaaacgcc gattgacccg ctcgatcgtg agctcccgcg tgaaggcaat gccggtgacc
     1441 gcgttctcga agagtaattc gagctccgtc gccacctcgc gcatcttcgc ctcggcgcgc
     1501 ttgcgctccg tcacgtcgat cgaggtgccc agtatgccga gcgccttgcc atcggcgtcg
     1561 cgcaggatgg cggcagacat caggctcgtg aatacttccc cattcttgcg ccggttctcg
     1621 acttcgctca cgcccccctg gcgttcgaac acgaggcggc ggatcgcttc cgcggattcg
     1681 ggatcggcgt agaggagatt ggcaggccgc ccgatcacct cctcgcgtgt ataaccgaac
     1741 gcccgctcgg cggcgggatt gaattccagt atgcgccgat cgcggtccac ggcgacgatc
     1801 gcggcaagag aagaatccac gatgctgcgc gcgtaatgcc ccgaagcgcg cacggcggaa
     1861 accgctttct tgagctcggt gacgtcctgg aatatgtaga gccgccaccc ttggggaagc
     1921 cccgccgacg gaccggccga gcgcaccaga aagacttctg cctcggacag cggcaactcg
     1981 aattcgtggg gggccgcgtc agactccggg accggaaacg gtgcattctg gatgggaaag
     2041 agcttccagt agacgcgccc aatcagattc ttgaccggca cgccggcccg ctcggcgtac
     2101 gcgcgattgg cgcgcacgat acagcccttg ttgtcatgca tgaacatcgg ctgggccagc
     2161 gcatccaccg tctgcgccca cgcctgccgc gactcctcga tgcggcgcga gcggaccgcc
     2221 tgctccttaa gcagcgtctg caggctttcc acttcggccc gcgccgcagc caattccgcc
     2281 tgcaggcgcg cgacctgcac ggactgatcc tggctttccc tcaccggtgc aacgctcacc
     2341 gcgtcctccc acgcatgccc cgaaacccga cggtaaatca caaagccacg aaactcaagc
     2401 gctgtcagcc tgccatggcg gtgcgccggt gcgcgaggga aaaccgcacg aaaccacatg
     2461 ggtgtgaggg cttgcgcaac tcaccgcctg cggtgcgg
//
LOCUS       DASURG010000479         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_491819, whole genome shotgun sequence.
ACCESSION   DASURG010000479 DASURG010000000
VERSION     DASURG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491819"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VFY80_08435"
     CDS             complement(<1..1170)
                     /locus_tag="VFY80_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX6007977.1"
                     /translation="MSWRAEIAEIERRRALAEACGGPEAVARHHAAGKLTVRERIARV
                     LDSGSFREVGKLAGRARYDDAGNLASFEPAPYVMGIGRIDGRPVAIGGEDYTIRAGTS
                     FGADRRKGGQGGFIEDLAYEYRIPLVNLIDGTGGTVNTARRKGYTVVPGYGQDGFERS
                     GDLMGMVPVVSAVMGTTAGGPSARAILAHWSVMVRGSGQIFAAGPPVVERAFGETLTK
                     EALGGAKVAVDTAGTIDNAAADEEECLRQIRRFLSYMPTNVWELPPETPCEDPVDRCE
                     DALADIIPRSPRQAYSMKKLLELVVDRGSVFEIQPTFGRAVITALARMNGRVVGIVAN
                     DPMHGGIVDHKAARKQGHFVELCDAFHIPLIFFADVPGLMVGLESEANALLREGVA"
     gene            complement(1185..1406)
                     /locus_tag="VFY80_08440"
     CDS             complement(1185..1406)
                     /locus_tag="VFY80_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEX6007978.1"
                     /translation="MARIEIKSEITGTVWQVQAKPGDKVESGDTLIVIESMKMEIPVI
                     TEDAGTVKEILVKQKDPVAEGQVVAVLED"
     gene            1617..2348
                     /locus_tag="VFY80_08445"
     CDS             1617..2348
                     /locus_tag="VFY80_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6007979.1"
                     /translation="MNANWEGARPAALTQSLPEQIAAQLSTRIVSGHYAPGQRIMEQA
                     IAAEFAVSRGPVREALRLLERDGLVTILPRRGALVTNLSTSEVKEIFDIRAVLNGLRD
                     RELAENPDRMHVLPALETEVAKLARYARQPEMGDAYIDTVFALNRMLTQASHNRRLRT
                     ILDSLALQTLRYSRLGLSTPQRRRQSVHHWQKLVKAIRDGDGTAAERAAQQRVLDSRD
                     AAIRLLEHADGARPATRETRSRRAA"
     gene            2371..3174
                     /locus_tag="VFY80_08450"
     CDS             2371..3174
                     /locus_tag="VFY80_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6007980.1"
                     /translation="MNPPQLTDAALTVEDRVALLTFKRDDVRNALTSTALVDDIPNAI
                     EWANAHPEVSVLVMTGEGSAFSAGGNIKTMGERSKAPPHILQQNYRSGIQRIPRALEA
                     AEIPVIAAVNGPAIGAGFDMANMCDIRIGSTNAQFGETFVNLGIIPGDGGAWFLEKLL
                     GYQRAAELALTGRIVKAEEAKAIGILLEVTSPGELMPRTLEIARRIAAKPPLSVRYCK
                     KLLKLAQRQNLEEHLEVCAAYQAICHKTEDHAEALAAFFEKRAGRFQGK"
     gene            3171..3866
                     /locus_tag="VFY80_08455"
     CDS             3171..3866
                     /locus_tag="VFY80_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007981.1"
                     /translation="MKLPHTAARLCLALLVIAVTSAEAQQAQSTKLPPVDEAAKDASW
                     VSFRRQLLNALAKHDRKFLLSIVDRNIRNPLDVPRGIATFRKQWDLDADDSPVWRELP
                     AALFLGSAWLKPEKGARQLCAPYVAVKWPDDIDPFDHGAITAREALVKSAPASDADTV
                     ATLSYDIVPVTDWEVPDRAADAKQKWVKIRLKDREAYVPEEQIRSPIEHRACFVKTES
                     GWRMVAFVVGLEK"
     gene            3881..>4449
                     /locus_tag="VFY80_08460"
     CDS             3881..>4449
                     /locus_tag="VFY80_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6007982.1"
                     /translation="MQRAVCLIAGLLMVGSAYSLDLQAHRGGRGLMPENTLPAFAHAL
                     ALGVTTLELDCGVTRDGVVVVSHDPMLNPDITRGPDGAWIGQLGPPIFHLTYDALRRY
                     DVGRIRPGSAYAARFTGQQPVDGTPIPRLAEVFDLARRAGNNTVRFNIETKISPDHPE
                     RTIDAVTFARALVTEVRAAGMTDRVTIQS"
BASE COUNT          788 a         1545 c         1388 g          728 t
ORIGIN      
        1 agccacaccc tcgcgcaata gggcgttcgc ttccgactcc agccccacca tgaggccggg
       61 cacgtcggcg aagaagatga gcggaatgtg gaatgcgtcg cacagttcga cgaaatggcc
      121 ctgcttgcgt gccgctttgt gatcgacgat tccgccgtgc atcggatcgt tcgccacgat
      181 tccgaccacc cggccgttca tccgcgcgag cgccgtgatg acggcgcggc cgaaggtggg
      241 ctggatttcg aacaccgagc cgcgatcgac caccagctcg agaagctttt tcatgctgta
      301 ggcctggcgc ggcgagcgcg ggatgatgtc ggcgagcgcg tcctcgcacc ggtccaccgg
      361 atcttcgcag ggcgtctccg gcggcagctc ccagacgttg gtcggcatgt aggagagaaa
      421 ccgccggatc tgccgcaggc actcctcttc gtccgccgcg gcgttgtcga tcgtcccggc
      481 agtgtccacg gccaccttcg cacctcccag cgcttccttc gtgagcgttt caccgaatgc
      541 gcgttcgacg accggcgggc cggcggcgaa gatctgcccc gagccccgga ccatgaccga
      601 ccagtgtgcg aggatcgcac gcgcggacgg tccacccgcg gtcgtgccca tcaccgcgct
      661 cacgacggga accatgccca tgagatcgcc cgagcgctcg aaaccgtcct gtccatagcc
      721 cggcaccacc gtatagccct tgcgcctcgc agtattgacc gtcccgccgg tgccgtcgat
      781 caaattgacg agcgggatgc gatactcata ggcgaggtct tcgatgaagc caccctgccc
      841 gcccttgcgc cggtcggctc caaaactggt gcccgcgcgt atggtgtagt cctcgccccc
      901 gatcgccacc ggccggccgt cgatgcgacc gatgcccatg acgtacggcg cgggctcgaa
      961 gctcgcgagg tttcccgcat cgtcgtagcg cgcccgaccg gcaagcttgc ccacctcgcg
     1021 gaacgacccg gaatcgagga cgcgggcgat gcgctctcgc accgtgagct tccccgcggc
     1081 gtggtgcctc gccaccgcct cgggtccgcc acacgcttct gcaagcgcac ggcggcgttc
     1141 gatttccgca atttccgctc gccagctcat cgcgcggcgc tgcttcagtc ctcgagaacc
     1201 gcgacgacct gaccctcggc gacgggatcc ttctgcttca ccagaatttc tttcaccgtc
     1261 cctgcgtcct cggtgattac cggaatctcc atcttcatcg attcgatcac gatcagcgta
     1321 tcgcccgact ccaccttgtc tcccggcttg gcctgcacct gccataccgt gccggtgatc
     1381 tcggacttga tctcgattct ggccatggtt ctgcagctcc tcgatagtgc gctatgggtt
     1441 gacgacgccc ggcggagaat gcggatcgga acggcgtcag ggggcgccgg acaggggcga
     1501 ggggcatcgt actcggcgag cgcgcatgtt gtcaagaaga ttgttgacaa tcttacacgc
     1561 ggagactatc gtgcgcgatc atgataccga cacgggccgc gccggcggcg cacgcagtga
     1621 acgcaaactg ggagggtgcc cgacccgccg cgctgaccca gtcccttccg gaacagattg
     1681 ccgcccagct ctcgacgcgc atcgtctccg gccactacgc gccgggccaa cgcatcatgg
     1741 agcaggcgat cgcggcggag ttcgccgtga gccgcgggcc cgtgcgcgag gcgctgcgcc
     1801 tgctcgaacg ggacgggctc gtcacgatcc tgccgcgacg cggcgcgctc gtgacgaacc
     1861 tcagcacctc ggaagtgaag gaaatcttcg acatccgcgc cgtgctgaac ggcctgcgcg
     1921 accgcgagct cgccgaaaat ccggaccgca tgcacgttct gccggcgctc gaaacagagg
     1981 tcgcgaagct cgcgcgctat gcccgccagc cggaaatggg cgatgcctac atcgatacgg
     2041 tctttgcact gaaccggatg ctcacgcagg cgtcgcacaa ccggcgcctg cgcaccatac
     2101 tcgactcgct cgcgcttcag actttgcgct attcgcggct ggggctctct acgccgcagc
     2161 gccgtcgcca atctgtccac cactggcaga aactggtaaa agcgatccga gacggagacg
     2221 gcaccgccgc ggagcgcgcc gcccagcagc gggtgctcga ttcacgcgac gctgcgatcc
     2281 gcctgctgga acacgcggac ggcgcgcgtc cggcgacacg cgagacgcgg tcacggcgcg
     2341 ccgcctgatc gacacgagga ttggcacgac atgaacccgc cccaattgac cgatgcggcg
     2401 ttgaccgtcg aggatcgcgt cgcgctgctc acgttcaagc gcgacgacgt gcgaaacgcg
     2461 ctcaccagca ctgcgctcgt ggacgacatc ccgaatgcca tcgaatgggc aaacgcccat
     2521 cccgaggtct cggtgctcgt catgacaggc gaaggcagcg ccttctcggc cggcggcaac
     2581 atcaagacca tgggcgagcg ctcgaaggcg ccgccccaca tattgcagca aaactaccgt
     2641 agcgggatcc agcgcatccc gcgcgcgctc gaggcggcgg agattccggt gatcgcggca
     2701 gtgaatggcc cggccatcgg agccggcttc gacatggcca acatgtgcga tatccgcatc
     2761 ggttccacca acgctcaatt cggcgagacc ttcgtcaacc tcggcatcat ccccggggac
     2821 ggtggtgcgt ggtttctcga aaaactgctc ggctaccagc gcgccgccga actcgctctg
     2881 accgggcgca tcgtcaaagc ggaggaagcg aaggcgatcg gcatcctgct cgaagtgacg
     2941 agccctgggg agctgatgcc gcgaacgctc gaaatcgcgc gccggatcgc ggcaaagccg
     3001 ccgctctcgg tgcgctactg caaaaagctc ctcaagctcg cgcagcgcca gaatctcgaa
     3061 gagcacctcg aggtgtgcgc cgcctatcag gcgatctgtc acaagacgga ggatcacgcg
     3121 gaagcgctcg ccgcgttctt cgaaaagcgc gcgggccgct tccagggcaa atgaagcttc
     3181 cgcacaccgc ggcccgcctc tgcctcgctc ttctggtgat cgcggtcacg agcgcagaag
     3241 cgcagcaggc acaatcgacg aagctgcctc cggtggacga agcggcgaag gacgcttcct
     3301 gggtgagctt ccgcaggcag ctactcaacg cgctcgccaa gcacgaccgc aagttcctgc
     3361 tctccatcgt ggaccgcaac atccgcaatc cccttgatgt gccgcgcggc atcgccacct
     3421 tccgcaagca gtgggacctc gatgcggatg acagcccggt gtggcgcgag ctgccggcgg
     3481 cgctttttct cgggagcgca tggctcaagc ccgagaaagg tgcacggcag ctctgcgcgc
     3541 cctacgtcgc cgtgaagtgg ccggacgata tcgatccgtt cgatcacggc gccatcacgg
     3601 cacgcgaggc gctcgtcaag tcggccccgg catccgatgc cgataccgtt gcaaccttgt
     3661 cctatgacat cgtgccggtc accgactggg aagtcccgga tcgcgccgcc gacgcaaagc
     3721 agaagtgggt aaagatccgg ctgaaggaca gagaagccta cgtgccggaa gagcagatcc
     3781 gcagccccat cgagcatcgc gcgtgtttcg tgaagacgga gagcggctgg cgcatggtcg
     3841 ccttcgtggt cggcctggaa aaataaccgg caatcggcgc gtgcagcggg ccgtttgcct
     3901 catcgcgggc ttactgatgg tcggttccgc ctacagcctc gacctgcagg cacaccgcgg
     3961 cgggcgcggg ctcatgccgg aaaacacgct ccccgcgttt gcgcacgcgc tcgcactcgg
     4021 cgtcaccacg ctcgagctcg attgcggcgt cacgcgtgac ggcgtcgtgg tcgtgagcca
     4081 cgaccccatg ctcaatcccg acattacccg ggggccggac ggcgcgtgga ttgggcaact
     4141 cggcccgccg atcttccacc tgacctacga cgcgctgcgc cgctacgatg tgggccgcat
     4201 ccgtcccggg tcggcctacg ctgcgcgctt tacaggtcag cagccagtgg atggcacgcc
     4261 cataccgcgg cttgccgagg tgttcgatct cgcccgccgc gccgggaaca atacggtgcg
     4321 cttcaatatc gagaccaaga tctcgccgga tcatcccgag cgcaccatcg acgcggtgac
     4381 ctttgcccgc gcactggtta cggaagtgcg cgcggccggg atgaccgacc gcgtcacgat
     4441 ccagtcgtt
//
LOCUS       DASURG010000480         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_696623, whole genome shotgun sequence.
ACCESSION   DASURG010000480 DASURG010000000
VERSION     DASURG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696623"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VFY80_08465"
     CDS             complement(<1..1060)
                     /locus_tag="VFY80_08465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6007983.1"
                     /translation="MNDSSDAYAELREGVRALCNEFDSAYWQKVDEARGYPDAFVEAL
                     TKAGWLSALIPEEYGGSGLSLTAASVIMEEINRSGGNSGACHGQMYNMGTVLRHGSEA
                     QKRQYLPKIASGELRLQSMGVTEPSAGTDTSKIKTVALRKGDRYIVNGQKVWTSRVQH
                     SELMILLARTTPLAEVKKKSEGMSIFIVDLREALQKGMTIRPIRNMVNHETNEVFFDS
                     LEIPAENLIGEEGKGFRYILDGLNAERILIAAECIGDGYWFIDRASDYARERVVFDRP
                     IGKNQGIQFPIARAYVNVEAANLMRYKAAGLFDEKKPCGAEANMAKLLGADASWEAAN
                     VCLQTHGGFGFAAEYDVER"
     gene            1201..1776
                     /locus_tag="VFY80_08470"
     CDS             1201..1776
                     /locus_tag="VFY80_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6007984.1"
                     /translation="MKNATRGAASPSRPAAVSAALVASWPEEIHQVLSELKIRQVAYV
                     PDSGHARLIELARADRSMRAVPLTTEEEGVALAVGAWLGGEKSVLLLQSSGVGNCINM
                     LATIEECRVPLLMLVTMRGQWGEFNPWQVPMAQGAAPSLTAMGVVVQTVDDPHDVAST
                     IAAAARLAFNTSRAVAVLIGQRVIGAKEFGK"
     gene            1773..2369
                     /locus_tag="VFY80_08475"
     CDS             1773..2369
                     /locus_tag="VFY80_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6007985.1"
                     /translation="MKHPKPTLDRRPLTAEILKQRGKTLVVGGLGAPCWDIAAAGDDP
                     LNFYAWGGMGGTAMIGLGLALAQPSRRVLVITGDGDMLMGLGSLATIAAQRPKNLAIL
                     VQDNEHYGETGMQITHTRLGVDLAGMAKAAGFSTTGTIYTATQLKTWIPRLYRQPGPV
                     FAVIKVKADRAPMVLPPRDGTVLKNRFRHALLGDAAFQ"
BASE COUNT          436 a          815 c          768 g          437 t
ORIGIN      
        1 tgcgctcgac gtcgtattcc gcggcgaagc caaacccgcc atgcgtctgc aggcacacgt
       61 tcgccgcctc ccacgaggcg tcggccccga ggagcttcgc catgttcgct tccgccccgc
      121 acggcttctt ctcgtcgaac agcccggccg ccttgtagcg catgaggttc gcagcctcga
      181 cgttcacgta cgcgcgcgcg ataggaaact ggatcccctg gttcttgccg atcgggcggt
      241 cgaacaccac ccgctcgcga gcatagtcgc tcgcgcggtc gataaaccag taaccatcgc
      301 cgatgcactc cgcggcaatg agaatgcgct cggcgttgag cccatcgagg atatagcgga
      361 agcccttgcc ctcctccccg atcaggttct cggccggaat ctcgagagag tcgaagaaca
      421 cttcgttcgt ctcgtgattc accatgttgc gaatcggccg gatcgtcatc cctttctgta
      481 gcgcctcgcg cagatcgacg atgaagatcg acatcccttc ggacttcttc ttgacctcgg
      541 caagcggcgt ggtgcgcgca aggaggatca tgagctccga gtgctgcacg cgcgaagtcc
      601 ataccttctg gccgttcacg atgtagcggt cgcccttgcg tagcgcgaca gtcttgatct
      661 tcgacgtgtc cgtgcctgcg ctcggctcgg tgacgcccat cgactgcagg cgcagctcac
      721 cgctcgcaat cttcggcaga tattggcgct tctgcgcctc cgacccgtga cgtagcaccg
      781 tgcccatgtt gtacatctgc ccgtggcagg cgccggagtt gcccccggag cggttgatct
      841 cctccatgat gaccgaggcg gcggtgagcg agagccccga gcccccgtat tcctcgggaa
      901 tgagcgcgga gagccagccc gctttggtca gcgcttcgac gaacgcatcg ggatagccgc
      961 gcgcttcgtc taccttctgc cagtaggcgc tgtcgaactc gttgcacaac gcgcgcaccc
     1021 cttcgcgcaa ttcggcatag gcatcggagg aatcattcat ggcgggatac gtgcgagagg
     1081 acgcagccga ttatagcagc gcggtgcggc cgcaagcctg cggggcattg tcgcgggcgc
     1141 agttaggcac cacaatacgc gctccagcgc atccaacaac ccacgcgaag gacaacaagc
     1201 atgaaaaacg ctacgcgcgg cgcggccagt ccctcgcggc ccgccgctgt atcggccgct
     1261 ctcgtcgcat cgtggcccga agagatccat caggtgctct cggaactcaa gatccgtcag
     1321 gtggcctatg ttccggactc cggccacgcg cgcttgatcg agctcgcgcg tgcggatcgc
     1381 agcatgcgcg cggtcccgct caccaccgaa gaggaaggcg tcgcgctcgc agtcggtgca
     1441 tggctcggcg gcgagaagag cgtattgctg ctgcagtcga gcggcgtggg gaactgcatc
     1501 aacatgctgg cgacgatcga agagtgccgc gtgccgctcc tcatgctcgt cacgatgcgc
     1561 gggcagtggg gcgaattcaa tccatggcag gtgccaatgg cgcagggcgc cgcgccctcg
     1621 ctcacggcga tgggcgtcgt agtgcagacg gttgacgatc cccacgatgt cgcaagcacg
     1681 atcgccgccg ccgcgaggct cgcgttcaac acttcgcgcg ccgtcgccgt gctcattggg
     1741 cagcgcgtaa tcggcgccaa ggagttcgga aaatgaagca ccccaagcct acactcgatc
     1801 gtcggccgct tacggccgaa atcctgaaac agcgcggcaa gacgctcgtg gttggcggac
     1861 tcggcgcgcc gtgctgggac atcgccgccg caggcgacga tccgcttaat ttctacgcgt
     1921 ggggcggaat gggcggcacc gccatgatcg gtctcgggct tgcgcttgca cagccgtcgc
     1981 ggcgcgtgct cgtcatcacc ggcgatggcg acatgctgat gggcctgggc tcgcttgcca
     2041 cgattgccgc gcagcggccg aagaatctcg cgatactggt ccaggacaac gagcactacg
     2101 gtgagacggg gatgcagatc acgcacacgc ggctcggcgt ggatctcgcg ggcatggcga
     2161 aagccgcggg attctcaaca accggaacca tctacaccgc cactcaactg aaaacctgga
     2221 taccgcgact ctatcgccag ccggggccgg ttttcgcggt catcaaagta aaagcggacc
     2281 gcgcgccgat ggtcctgccg ccgcgcgacg gcaccgtgct caagaaccgc ttccgccacg
     2341 cgctcctggg cgacgcggcg tttcagtgag cgctgtcact actgccgttc gtgtcgagcg
     2401 aagtcccgtg ggccttctgt ggtgttcagc gatcgcccgg cgcaggtcaa caatcg
//
LOCUS       DASURG010000481         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1104760, whole genome shotgun sequence.
ACCESSION   DASURG010000481 DASURG010000000
VERSION     DASURG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104760"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            623..1732
                     /locus_tag="VFY80_08480"
     CDS             623..1732
                     /locus_tag="VFY80_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6007986.1"
                     /translation="MSASKWGDLIPRVVETNEDGTAYDRWSIYGRVSDLDICNCPALM
                     GEPFPTFPKRWEEVPATAYEPRARLAALDRDGIDAEVLFPNPPGGTFFEYGDRDFELD
                     VVRAYNDALSEWARASDRYLPLAIIAYLSEPHIIAREIERAASAGHRGINCQGEMPAG
                     LPHLTDPYWYPLWDVCQALDLPVHIHGSAGVRAGASVRKWSGYTPRQAHSAMTATSAV
                     TPAQVVPQLIFSGMMQRFPRLKIVFAEAGIGGLNYVLAACDHEWQSRRLWSESLTTRP
                     SETVRRQVYVNFWFEAEGIKLRHDIGIDNIMWESDFPHVASYYPRSWHEVHRVLEGVP
                     PEDRRKLLYENAIRLYRIDAAWPEKTISGDAQPWS"
     gene            1723..>2084
                     /locus_tag="VFY80_08485"
     CDS             1723..>2084
                     /locus_tag="VFY80_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007987.1"
                     /translation="MELKYGFISVDDHVQEPPDVWTARLPRGRWGSRVPHLERALDGA
                     EHWVVDGKILLGGGAGKAGAFMSDRNCEPRRWDEVPAAAYEPAERLKAMDAAGVDYSV
                     LYPTVAGLAGEAFARIED"
BASE COUNT          388 a          614 c          669 g          413 t
ORIGIN      
        1 gtgggcggcg tcgagtagct cctcgatcca ctcccgagga atttcggttt gattgtactc
       61 cttcaccgag cggcgcgtct tgattgcggt gagcgcatca gcgagagggc gctccgttgt
      121 ggttgccatg atcctacctc ctgattctgt ccgatttata tctataggta tgtatagaaa
      181 attcagacgg cgagcaacat cgcctgaccg gagagctgga cagctggcag aaagcagcga
      241 gcagccggca cggagcagag ggcagggagg agaaactgaa gcgttccggt tgctccaatc
      301 gtttcagtcg ttcccagtgt taaagacgtt cgcttggtgg cggtttggag cggttggaat
      361 ccttccagct tgaacggctt ggactacctg aacgttttga acgcagcgca gcgattgaac
      421 ggcttgaacg ccttgaacgg gcttcggtac tctttgccag cttgacattc ttgacggtgg
      481 ggcgttatcc ctagcacatc atgcacgtcg ctcactcgaa tcatgattgc gtttcccttg
      541 gggaggaagg atgcaactta agtacggact gatcagtgcc gattctcacg cggcatttga
      601 cccggccacc tacaccagcc gaatgtcggc aagcaagtgg ggagatttaa tcccacgcgt
      661 agtcgaaacg aacgaggacg gcacagctta cgaccgctgg tcaatctacg gcagggtttc
      721 cgacctcgac atatgcaact gccccgcact gatgggcgag ccgttcccga ccttcccgaa
      781 gcgatgggag gaagtgcccg cgactgccta tgagccgcgg gcgcgtctcg ccgcgctcga
      841 ccgcgacggc atcgatgcgg aggtgctgtt tccaaacccg ccgggcggaa cgttcttcga
      901 atacggcgat cgcgacttcg agcttgacgt cgtgcgcgcc tataacgatg cgctgtccga
      961 gtgggcgcgc gccagcgatc gctatctgcc tctcgccatc atcgcctatc tcagcgaacc
     1021 gcacatcatc gcgcgcgaaa tcgagcgcgc ggcgtcggcc ggccaccgcg gcatcaattg
     1081 tcagggcgag atgccggccg ggcttcccca tctcaccgat ccgtactggt atccgctctg
     1141 ggatgtttgc caggcactcg atctgccggt gcacattcac ggctccgcag gcgtgcgcgc
     1201 cggcgcgtcg gtgcggaaat ggagcggcta tacgccgcgc caggcgcatt cggcgatgac
     1261 ggccacgtcg gcggtaacgc cggcgcaggt ggtcccgcag ttgatttttt ccgggatgat
     1321 gcagcgcttc ccgcgcctga agatcgtgtt cgcggaggcc ggcatcggcg ggctcaacta
     1381 cgtgctcgcc gcctgcgatc acgaatggca atcgcgccgg ttgtggagcg agagcctgac
     1441 gacccgcccg agcgaaacgg tgcgccggca agtgtatgtg aatttctggt tcgaagccga
     1501 agggatcaaa ctgcgccacg acatcggcat cgacaacatc atgtgggagt cggactttcc
     1561 ccatgtggct tcgtactacc cgcgttcctg gcacgaggtc catcgcgtgc tcgaaggcgt
     1621 gccgcccgag gatcgccgca agctgctcta cgaaaatgcc atacgtctat accgcatcga
     1681 tgcggcttgg ccggagaaaa ccatctcggg ggatgcgcaa ccgtggagct gaagtacggt
     1741 tttatcagcg tcgacgatca cgtgcaggaa ccgcccgacg tttggaccgc tcggttgccg
     1801 cgcggccgtt ggggttcgcg ggtgccgcat ctcgagcgcg cattggacgg agccgagcat
     1861 tgggtcgtcg atggaaaaat tttactcggc ggcggcgccg gcaaagccgg cgccttcatg
     1921 agcgatcgca attgcgagcc gcgccgatgg gacgaagtcc cggcggcggc ttacgagcct
     1981 gcggagcgtc tgaaggcaat ggacgccgcg ggtgtggatt attcggtgct ctatcccacc
     2041 gtggcggggc ttgcgggcga ggcgttcgcc cggatcgagg acag
//
LOCUS       DASURG010000482         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1104835, whole genome shotgun sequence.
ACCESSION   DASURG010000482 DASURG010000000
VERSION     DASURG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104835"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFY80_08490"
     CDS             <1..377
                     /locus_tag="VFY80_08490"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX6007988.1"
                     /translation="FREEFIQLVRTYHDRLGALYDTALAEAEMRRRKEETISQMVRDY
                     QALKASWGGFTGYDRWFDRRPNNAQIASVAIYTRLVPAFRALLREHDGDLPAFYAAVR
                     KLAEMPKEARTAALEARGERGG"
     gene            432..1061
                     /locus_tag="VFY80_08495"
     CDS             432..1061
                     /locus_tag="VFY80_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX6007989.1"
                     /translation="MQFASASFGDNYAIPPEYAFCAPDRASRVKMSQNSNPHLTWSGL
                     PAGTKSLALICHDYDVPSKADDVNQEGRTIPASLPRVDFYHWVLVDIDPKINIIKAGE
                     FSNGITPRGKPGPDAPRGTRQGINDYTKWFASDKDMAGNYFGYDGPCPPWNDTIPHHY
                     VFTLYALDIEKCPVSGTFTGPDVLKAMEGHVLDKASFTGLYSLNPNVKV"
     gene            1131..3667
                     /gene="hrpA"
                     /locus_tag="VFY80_08500"
                     /pseudo
     CDS             1131..3667
                     /gene="hrpA"
                     /locus_tag="VFY80_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524161.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
BASE COUNT          687 a         1185 c         1166 g          629 t
ORIGIN      
        1 agtttcgcga ggaattcatc cagctcgttc gcacctacca tgaccggctt ggcgcgcttt
       61 acgatacggc gcttgcggaa gcggagatga gacgccgcaa ggaagagacc atttcacaga
      121 tggtgcggga ctaccaggcc ctcaaagcga gctggggcgg gttcacggga tacgaccggt
      181 ggttcgaccg tcggccgaac aacgcgcaga tcgcatctgt cgcgatctac acccgtctcg
      241 tgccggcgtt tcgtgcgctc ttgcgcgaac acgacggcga tcttccggcg ttctatgccg
      301 ccgtgaggaa gctcgccgaa atgccgaaag aggcacgcac cgccgcgctg gaggcgcgtg
      361 gcgagcgcgg gggttgagac cgctgcggcg atgctgtaat ttgcgacggt tacgcgaaga
      421 aggaggctcg tatgcaattc gccagtgcca gcttcggcga caactacgcg attccgcccg
      481 aatacgcgtt ctgcgcgccc gatcgcgctt cacgcgtgaa gatgtcgcag aactccaatc
      541 cgcacctcac gtggagcggc ctgccggcgg gaacgaaatc gctcgcgctc atctgtcacg
      601 attacgacgt gccgagcaag gccgacgacg tgaaccagga agggcgcacg attcccgcgt
      661 ccctcccgcg cgttgatttc taccactggg tgcttgtgga catcgacccc aagatcaata
      721 tcatcaaggc gggcgagttc tcgaacggca tcacaccgcg cggaaagccg ggccccgatg
      781 cgccgcgcgg cacgcgccag ggcatcaacg actacaccaa gtggttcgcc tccgacaagg
      841 acatggcggg aaattacttt ggttacgatg ggccgtgtcc gccgtggaac gacacgattc
      901 cgcaccatta cgtgttcacg ctctacgcgc tcgatatcga gaagtgccct gtcagcggca
      961 cgttcacggg acccgatgtg ctgaaggcga tggaagggca cgtgctcgac aaggcgtcct
     1021 tcaccggact ctacagtctc aaccccaacg tcaaggtgtg atcccgcgtg ggccagacca
     1081 aggccggtct tgaccggcct ttttattttg gcgcctcgaa attcgacgcg gtgcaacggc
     1141 gcgccaacct gccgcgccca acgtatcccg acgagttgcc ggttgtcgcg cttcgcaaag
     1201 aaattgcggc cgctgtcgcc gcgcatccgg ttgtcatcgt ttgtggcgaa accggctcgg
     1261 gaaagacgac gcagcttccc aagatctgcc tcgagctcga acgcggcgtg aacgggctca
     1321 tcggtcacac ccagccacga cgcatcgcgg cgcgcacggt cgcagcccgc atcgctgccg
     1381 agctcaaagc cccctcggcc acgccgtggg ctacaaggtg cgcttctccg accggctttc
     1441 ggagcacacc tacatcaaag tgatgaccga cggcatcctg ctcgcggaga cgcagggcga
     1501 ccgactcctg cggaactacg acacgctcat catcgacgaa gcgcacgagc gcagtctcaa
     1561 tatcgacttt ctgctggggt acgtgaagcg ccttctgccg cggcgtccgg aactcaagct
     1621 gatcatcact tcggcgacga tcgacgccga gcgcttctcg cgccattttg acggcgcgcc
     1681 cgtgatcgag gtgtcgggcc gcacctaccc ggtggaaata cgctaccggc cgctcgacga
     1741 ccccgattcg gacaacgaaa gcacgctcga agaggcaatc gtcgatgcgg cggacgaact
     1801 cgcgcgcgag ccgggcggcg gggacatcct cgtattcctg ccgggcgagc gtgaaatccg
     1861 ggacaccgcc gaagcgctgc ggaagcacgc gttcggacgc gtttctcctg caatgcgcca
     1921 cgtcgagatc ctgccgctct atgcgcggct ctcgttcgaa gagcagcagc gcgtgtttcg
     1981 gcgaagcgcg cctgcgagtc gaatcgtgct cgcgaccaac gtcgcggaga cgtcgctcac
     2041 ggtcccgggc atccactacg tggtcgatac cgggctcgcg cgcatcaacc gctacagcta
     2101 tcgcaacaag gtcgagcagc tccaggtcga gaagatttcg cgtgcggctg cggaccagcg
     2161 cgccgggcgc tgcgggcgcg tcggagcggg cgtgtgcatc aggctctatt ccgaagacga
     2221 ttacgccgca cggcctcaat ataccgatcc agagatcctg cgctcttcgc tcgcgcacgt
     2281 catcctgcgc atgaaggcgc tccggatcgg cgacgtcgag gattttccgt ttctggagcc
     2341 gccggcaccg cgcatggtcg cggacggcta ccagctcctg accgaactca acgcggtgga
     2401 cgagaaacgc aacctcaccg ctctgggcgt gcgactcgcc aggtttccca tcgatccccg
     2461 tatcgcgcgc atgattctgg ccgcgcaaag cgaagcctgt ctgaccgaaa tcctgatcat
     2521 cgccgcggcg ctcacgatcc aggatcctcg cgagcgcccg ctagagaagg cggaagccgc
     2581 ggatcgcgcg catgagccgt ttcgcgatga gcgttccgac ttcatgggat atctcgagct
     2641 gtggaaattc ttcgacggtg cgctcaagca caggaagtcg catcgcaagc tgcaggcgct
     2701 ggtgcacgag cattttcttt cccatcgccg gctgcgcgag tggcgcgacg tccatgcgca
     2761 gctcgccgcg ctcgcgggcg acatcgggct gcaccccaat gagaagccgg ccggctattc
     2821 cgaaatccat cgggcgcttc tcgccggcct cttaggcaac atcggattca agaatgaaga
     2881 cggcgaatac gtgggggcgc gcggcatcaa atttgccatc tttccgggct cgggactgcg
     2941 caagtcgcag cccaagtggg tgatggcggc ggaagtcgtc gagacggcgc ggctctatgc
     3001 gcgcacggtg gcgcggatcg agccggagtg gatcgagccg ctcgcgcgcc atctcgtaga
     3061 gcgccattac gtcgatccgc attgggagcg ggagagggcg atggtcgtcg cgttcgagcg
     3121 tgtcacgctc tatgggctca ccatcgtgtc gcggcggcgc gtgcattacg ggccgatcga
     3181 tgccgcggca tcgcgcgaaa tcttcatccg ccaggcgctc gtaggaggcg agtacgagac
     3241 gcgcgccccg ttcttcgagc acaaccggcg cctcatacgg gacgtgcagg cgctcgagca
     3301 caaggcgcgg cggcgcgacg tgctggtgaa cgacgaggcg atctatgctt tctacgacgg
     3361 gctcattccg gaaggcatcg tgaatggcgc cggattcgag aagtggcggc gcgaagccga
     3421 gcgcgcggat ccgcggctcc tccacctcac gcgcgaatac ctcatgcggc acgccgcagc
     3481 ggacatcacc gaggcgcaat ttccggaaac catgcgcgtc ggggaagcgg atctccggct
     3541 tcgttaccgc ttcgagccgg gacatccgct cgacggcgtg acggcgacgg tcccgctgca
     3601 tctgctcaat acgctggata ccgcctgctt cgagtggctc gtgcccggca tggtgagaga
     3661 gaaagtg
//
LOCUS       DASURG010000483         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_798640, whole genome shotgun sequence.
ACCESSION   DASURG010000483 DASURG010000000
VERSION     DASURG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798640"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFY80_08505"
     CDS             <1..201
                     /locus_tag="VFY80_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007990.1"
                     /translation="RLRGPGELLGARQSGVPLLRFADLTADVDLLESARNAADALVRE
                     NAPAVRAHLDRWLGARHEFLKV"
     gene            204..749
                     /locus_tag="VFY80_08510"
     CDS             204..749
                     /locus_tag="VFY80_08510"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEX6007991.1"
                     /translation="MSLPLRDRRWLRRPPHSTGTVRAWLVDRGSLTARIQARCPAFSV
                     KLVFQGRGRVDRDETFLVPPGRRGPALVREVYLYCRRTPVVFAHSVFNPRSLRGAWRR
                     LARLGTRPLGAALFADPRVHRYPLRQRKLNRQHELYRRACRALNARPPFLWARRSVFT
                     LHNSPILVTEVFLPGIDKLRA"
     gene            746..1621
                     /gene="ubiA"
                     /locus_tag="VFY80_08515"
     CDS             746..1621
                     /gene="ubiA"
                     /locus_tag="VFY80_08515"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX6007992.1"
                     /translation="MTLKEKLDAYEKLMRLDKPIGILLLLWPVLWGLWLAAPGTLRLD
                     IVLIFVLGTILTRSAGCIINDYADRDFDPHVKRTRERPLAAGRVTTTEALVLAAVLFL
                     LAFVLVLFLNRLTILLAFVALFLAISYPFTKRFFALPQAYLGIAFGISIPMAFAANHG
                     TIAPVAWVMLLANVFWAMAYDTEYAMVDRDDDVRIGIKSSAILLGRYDVPAVIACHTA
                     FLAIMVMVGMWLKLGVLYYVGLTLAAGFVLHQYQLIRDRDREKCFAAFLNNNWVGAAV
                     FAGIALDIFFRVRSA"
     gene            1632..>2038
                     /locus_tag="VFY80_08520"
     CDS             1632..>2038
                     /locus_tag="VFY80_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="catalyzes the decarboxylation of
                     3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate decarboxylase"
                     /protein_id="HEX6007993.1"
                     /translation="MKYRDLREFMTQLEARGELKRIGVEVDPRLEMTEICDRVLKARG
                     PALLFERPRGHAIPVLGNLFGTPERVARGMGQDSVAALRELGQLLAYLKEPEPPRGWK
                     DAWEKLPLLKQVLSMTPREVSGARCQEIVWEGK"
BASE COUNT          289 a          680 c          668 g          401 t
ORIGIN      
        1 cggctgcgcg ggccgggcga gttgctgggc gcgcgccaga gcggcgtgcc gctcctgcgc
       61 tttgccgatc tcactgccga cgtcgatctg ctcgaatcgg cgcgcaacgc cgccgatgcg
      121 ctggtgcgcg agaacgcgcc cgcggtgcgc gcgcatctcg atcgctggct cggcgcgcgg
      181 catgaattcc tgaaagtctg atcgtgagcc ttcccctccg cgaccggcgc tggttgcgcc
      241 gcccgcccca ttcgaccgga accgtccgcg cgtggctcgt ggaccgcggc tcgctcacgg
      301 cgcggatcca ggcccgttgt cccgcattca gcgtgaagct cgtcttccag ggccgcgggc
      361 gcgtcgatcg cgacgagacg tttctcgtcc cgccgggccg gcgcggtccg gccctcgtgc
      421 gcgaggtgta cttatattgt cgccgcacgc cggtcgtctt cgcgcacagc gtgttcaatc
      481 cgcgctcgct gcgcggagcc tggcggcggc ttgcgcggct cggcacccgg ccgcttggcg
      541 cagcgctctt cgccgatcca cgggtccacc gctatcccct gcgccagaga aagctcaatc
      601 gtcagcacga gctctaccgg cgcgcgtgcc gggcgctcaa tgcgcggccg ccttttctgt
      661 gggcgcggcg ctcggtattc acgctgcata attcccctat actggtgacc gaagtgtttc
      721 tgccaggcat cgataaactg cgcgcatgac gctcaaggag aagctcgacg cctacgagaa
      781 gctgatgcgg ctcgacaagc cgatcggcat cctgcttttg ctctggccgg tgctgtgggg
      841 gctctggctc gcggcgcccg gcactctgcg tctcgatatc gtcctcatct tcgtgctcgg
      901 cacgatcctc acgcgctccg ccggctgcat catcaacgac tatgccgacc gcgatttcga
      961 cccgcatgtg aaacgcacgc gcgagcggcc gctcgccgcg ggccgcgtga cgacgaccga
     1021 ggcgctcgtg ctcgcagcgg tgctctttct gctcgcgttc gtgctggtcc tgttcctgaa
     1081 ccggctcacc atcctgctcg ccttcgtggc gctcttcctc gccatcagct atcccttcac
     1141 caagcgcttc ttcgcgctgc cgcaggcgta tctgggcatt gcgttcggga tttcgatccc
     1201 gatggcgttt gcggctaacc acgggacgat tgcgccggtc gcgtgggtga tgctgctcgc
     1261 gaacgtgttc tgggcaatgg cctacgatac cgagtacgcg atggtcgatc gcgacgatga
     1321 cgtccggatc ggcatcaaat cctccgcgat tctgctcggg cgctacgacg tgccggcggt
     1381 aatcgcatgc cacacggcct ttctcgccat catggtgatg gtcggcatgt ggttgaagct
     1441 cggcgtgctc tattacgtcg ggctcacgct cgcggcaggg ttcgtcctgc accagtatca
     1501 gttgatccgg gaccgcgacc gcgagaagtg cttcgcggcg tttctcaaca acaactgggt
     1561 cggcgcggcg gtctttgccg gcatcgcgct cgacatcttc ttccgggtcc gctccgcgtg
     1621 agccctccgc gatgaaatac cgcgatctgc gcgaattcat gacgcaactc gaagcgcggg
     1681 gtgaactgaa gcgcatcggc gtcgaggtgg atccgcgtct cgagatgacg gagatctgcg
     1741 atcgcgtact gaaagcgcgt gggcctgcgc tgctttttga aaggccgcgg gggcatgcga
     1801 ttcccgtgct gggcaatcta ttcggcacgc cggaacgcgt cgcgcgtggc atgggtcagg
     1861 actccgttgc cgcgttgcgc gagctgggcc agcttctcgc ctacctcaag gagccggagc
     1921 cgcctcgggg ctggaaggac gcctgggaga agctgccgct cttgaagcag gtgctcagca
     1981 tgacaccgcg cgaagtgtcg ggcgcgcggt gccaggaaat cgtgtgggaa ggaaagga
//
LOCUS       DASURG010000484         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_697090, whole genome shotgun sequence.
ACCESSION   DASURG010000484 DASURG010000000
VERSION     DASURG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697090"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFY80_08525"
     CDS             complement(<1..221)
                     /locus_tag="VFY80_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6007994.1"
                     /translation="MPHALLVDDDRNTLAALSELVAREGFTVAIAATLQEARQRVSER
                     HPDVVLLDLMLPDGNGMELFDDVKSRSIT"
     gene            550..1227
                     /locus_tag="VFY80_08530"
     CDS             550..1227
                     /locus_tag="VFY80_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX6007995.1"
                     /translation="MAKRVTSSSRNRSKARSSPAESMGHPAEEVKSLDEARSEALECR
                     ACPLWRDTTQTVFGEGARDAAIVFVGEQPGDSEDRTGRPFVGPAGHMLDRALHEAGIA
                     RDAVYVTNAVKHFKYVARGKRRLHKKPADAEIDACHDWLARELEFVAPRLVIALGATA
                     ARSLLGRATPIEANRGRLLPFPGTTARLLITVHPSALLRIPDEFKAEAYRRFVADLKI
                     GAEFIHS"
     gene            1224..1955
                     /locus_tag="VFY80_08535"
     CDS             1224..1955
                     /locus_tag="VFY80_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007996.1"
                     /translation="MRRFPDVLRGWVHGLRLLVRRASPNKRRVSALAIGLALLGSVSY
                     IAYIEHEKRSLRRAIAELARDTGSRFHDALRTVADEGGSAQQRLDDHYFTIDRHLKRL
                     RSMDTAPAQDLARAADDYMLTVREIAKRLASGHRYRAEVLETVRALQVHMGEDKRTGA
                     WVTQAVQRRERLRTEYRAYRLTSEALVGLLNDYASSRARLAQHIDSAFMPEDSMALAV
                     RDLILKARQKIAEDVERAGALETYR"
     gene            complement(1960..2136)
                     /locus_tag="VFY80_08540"
     CDS             complement(1960..2136)
                     /locus_tag="VFY80_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6007997.1"
                     /translation="MKSAIAAVLFATTLSACVAVPYEPAPAYGYYGPTYYYGPPVASF
                     HFGYHSHRHHRYRH"
     gene            2347..3105
                     /locus_tag="VFY80_08545"
     CDS             2347..3105
                     /locus_tag="VFY80_08545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6007998.1"
                     /translation="MVAHNRVAVVTGAGSGIGRAAALALLRDGYRVGLAGRRAALLEQ
                     TASAAPEAADRALVAPTDVTDPESVTNLFSRVRDAFGRIDVLFNNAGANAPGVPFEDL
                     SFERWKTVVDCNLTGSFLCAQEAFRTMKNQDPRGGRIINNGSISAHAPRPDSAPYTAS
                     KHGVTGLTKTISLDGRKYDIACSQIDIGNAMTELAARMSRGVKQADGTIKVEPMIDVA
                     QVGEAVRYIAGLPLDVNIQFMTIMATKMPFVGRG"
     gene            3327..3554
                     /locus_tag="VFY80_08550"
     CDS             3327..3554
                     /locus_tag="VFY80_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX6007999.1"
                     /translation="MKSMKISAVAVLLTAMVAGCSNMTPRERDTAVGAAIGGVGGAAV
                     SGGSALGTLGGAAAGGFIGHQYGDDIRRKVD"
BASE COUNT          699 a         1132 c         1188 g          672 t
ORIGIN      
        1 tccgtgatgg agcgcgattt gacatcgtcg aaaagctcca tcccgttgcc gtcgggaagc
       61 atgaggtcga gcagcacgac gtcgggatga cgctcggaca cccgctggcg cgcttcctgc
      121 aacgtcgcag cgatcgcgac cgtgaagccc tcgcgcgcca cgagctccga gagagcggcc
      181 agcgtattgc gatcgtcgtc tacgagcagg gcgtgcggca tgagcgaagc ctcttgagca
      241 ggattgcgcc ggaattttca gttgcggcat tcgtgccgcg cctgccgcat tatcacgcgc
      301 tgaagcggca ctcggcagca tccccgttac gcattgcgga caacgctgtg aaccgctttt
      361 gtgcgcgaag ttcactttcc cggcccggag cgtacgcgac actcatcccc atttcgcggc
      421 cgtcagaaat tccacatgca cgtgctgttg cattccagcg acagcgttgc gtacgctaaa
      481 gtgtcgtcat caggcaacgc gcaacgagaa atgcgattga gtgtcgaaac aacgcgggta
      541 ggcaagtgca tggcgaagcg cgtcacaagc tcttccagga atcgcagcaa agcacgcagc
      601 tcgcctgcgg agtccatggg tcacccggcc gaagaggtca aatcgctcga tgaagcgcgc
      661 agcgaagcgc tcgagtgccg cgcttgccca ctgtggcggg acacgactca gaccgtgttc
      721 ggcgagggtg cgcgcgacgc ggccatcgtg tttgtcggcg agcagccggg tgacagcgag
      781 gaccgcaccg gcaggccgtt tgtcggtcct gccggtcaca tgctcgaccg cgcgctgcat
      841 gaagcgggca tcgcccgcga cgccgtatat gtcaccaacg cggtaaaaca cttcaagtac
      901 gtcgcgcgcg gcaagaggcg cctgcacaag aagccggccg acgccgaaat tgacgcatgc
      961 cacgactggc tcgcgcgcga gctcgaattc gtggcgccgc ggctcgtcat cgccctcggc
     1021 gcgacggccg cgaggtcact gctggggcgc gcaacgccga ttgaggccaa tcgcggtcgt
     1081 ctcctgccat ttcccggaac gacggcgaga ctcctcatca ccgttcatcc gtctgcgcta
     1141 ctgcgcatcc ccgacgaatt taaagcggaa gcctaccgtc gcttcgtcgc ggatctgaag
     1201 atcggggcgg agttcatcca ctcatgaggc gctttcccga cgttctgcgg ggatgggtgc
     1261 acggccttag gctgctcgtg cgtcgcgcaa gtcccaacaa acgacgggtg agcgctctgg
     1321 cgatcgggct cgcgctcttg ggctcggtgt cctacatcgc gtacattgag cacgagaaac
     1381 gctcacttcg gcgcgcaatt gcagagctcg cgcgggatac tggcagtcga tttcacgatg
     1441 cgcttcgcac ggtcgcggac gaaggcggat ccgcccagca gcgcctggat gaccattact
     1501 tcaccatcga tcgccatctc aagagactgc gttccatgga caccgcgccg gcccaagatc
     1561 tggcgcgcgc ggccgatgat tacatgctca cggtccgcga aattgccaag cgactcgcaa
     1621 gcggccatcg ataccgcgcc gaagtcctgg agacggttcg cgccctgcaa gtccatatgg
     1681 gagaagacaa acgcaccggt gcctgggtca ctcaggcagt gcaaaggcga gaaaggctgc
     1741 ggacggagta ccgcgcctac cggctcacga gcgaggcgct cgtcggcctc ctgaacgact
     1801 acgcatcatc gcgggcacgc cttgctcagc acatcgactc ggcgttcatg ccggaggaca
     1861 gcatggcgct cgcagttcgc gacttgatcc tgaaagctcg ccagaagatc gcggaagacg
     1921 tggagcgggc gggtgcgctc gagacctatc gttgatgcct cagtggcggt agcgatgatg
     1981 gcgatgactg tggtagccga agtggaagga cgccaccggc ggaccatagt agtaggtcgg
     2041 cccgtagtag ccgtaggcgg gcgccggttc gtacggcacc gcaacgcacg cgctcagggt
     2101 cgtggcgaac agcacagccg caattgcgct tttcatcgaa acctccttgt ccgtcgcccc
     2161 atgctatggg tgttcgacca caagataccg catggggtcg ccctgcatct gtaagttgtt
     2221 gtaaggattt gtaacggtgc tacggcgctc tcttcttttt tgttcggtgc ccgcgcactc
     2281 ggcattgcga cgacgccgcg ccgctgtgcg ctagcatagc gctccctcac agcaaaggag
     2341 actcaagtgg ttgcacacaa cagggttgca gtagttacgg gcgcaggaag tggcatcgga
     2401 cgagccgcgg cgctggcact gttgagggac ggctatcgcg tcgggctcgc cggacggcgc
     2461 gccgcgctcc tcgagcagac cgccagcgcg gcgcccgagg ccgccgaccg cgcgctcgtc
     2521 gcgcccaccg acgtgacgga tcccgaatcc gtaacgaatc tcttctcacg ggtgagagac
     2581 gcgttcggcc gcatcgatgt gctgttcaac aacgcgggcg ccaatgcgcc gggcgtgccg
     2641 tttgaagact tgtcgttcga gcggtggaag actgtcgtgg actgtaatct gaccggttct
     2701 tttttgtgcg cgcaggaggc gtttcgcacg atgaagaatc aggatccgcg cggcggacgc
     2761 attatcaaca acggctccat ctcggcacac gcaccgcggc cggattctgc cccgtacacg
     2821 gcaagtaagc acggtgtcac tggcctgacg aagaccatct cgctcgacgg ccgcaaatac
     2881 gatatcgcgt gcagccagat cgacatcggc aacgccatga ccgagcttgc agcgcgcatg
     2941 tcgcgcggcg tgaagcaggc cgacgggacc atcaaggtgg agccgatgat cgacgtcgcg
     3001 caggtaggag aggccgtgcg ctacatcgcc ggcttgccgc tcgatgtcaa catccaattc
     3061 atgacgatca tggcgacgaa gatgcccttt gtcgggcgcg gataacaccc gtgtcgtttc
     3121 cgggtgacgc aatgcgcgac aaaaaatcta acggtcgttt gaacggctgc tccgggccgc
     3181 aacgatatgc gcaggcctga cgtgagaaat cctcagctcg cggcgtttcg ctagaacccg
     3241 cgctccgcct gtcggtcttt catttggtgc gtggtccgtt tgtttgcggg cctttttaat
     3301 ggactcgaca gatggaggca cttgctatga agagcatgaa gatttccgcg gtcgcagtgt
     3361 tgctgacggc gatggtcgcc ggttgctcga acatgacgcc acgcgagcgg gataccgcag
     3421 tgggtgctgc gatcggcggc gtcggcggtg cggcggtgag cggcggcagt gccctgggta
     3481 cgctcggtgg tgcggcagcc ggaggattta tcggtcacca gtacggcgac gacattcggc
     3541 gtaaagtcga ctagcgccga acaggcaaac agagagaccg ggagcgatcc cggtcttttt
     3601 ttgtttcgcg tcaggccggc agctgcggcg gcgccgtcag gacttcggtc agcttgtccg
     3661 ggtccagcgg acgcgcatag atgtatccct g
//
LOCUS       DASURG010000485         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1412857, whole genome shotgun sequence.
ACCESSION   DASURG010000485 DASURG010000000
VERSION     DASURG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412857"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..377
                     /locus_tag="VFY80_08555"
     tRNA            302..377
                     /locus_tag="VFY80_08555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:335..337,aa:Thr,seq:tgt)
     gene            626..1588
                     /locus_tag="VFY80_08560"
     CDS             626..1588
                     /locus_tag="VFY80_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6008000.1"
                     /translation="MITVEHLTKAFGPKLAVNDVTFTVNRGEVLGFLGPNGAGKSTTM
                     RMVTGFIPPTSGSITVGGHDVLENPIPAKRMIGYLPENAPGYADMTVTGFLRFAAELR
                     GLHGDARKRAVNRAIELCFLQNVVYQSIDTLSKGYKHRTCLAQSLIHDPPILIMDEPT
                     DGLDPNQKHEVRNLIKRMGQDKAIIFSTHILEEVEAVCSRVIIIDRGKIVANGTPEEL
                     KLRSEMAGAVHLRVNDVPAAALQEELKRVKGAARSEILAERDGTVELRVYPDGTAARG
                     SLTRDVAALAAQQRWQVAEIHTEEGRLDEVFRAITLPDTVRAAA"
     gene            1689..>2192
                     /locus_tag="VFY80_08565"
     CDS             1689..>2192
                     /locus_tag="VFY80_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6008001.1"
                     /translation="MNAWANIKTIMKRELGGYFTSPIAYVFLVIFLLLTGFFTFTVGS
                     FFERGEASLVSFFTWHPWLYLFLVPAVGMRLWAEERRLGTMELLLTMPITTWQAIVGK
                     FLASWLFLALALALTFPVVITVNYLGDPDNGVIVAGYVGSLLLAGTYLAISCMTSALT
                     RNQVISFI"
BASE COUNT          432 a          691 c          675 g          394 t
ORIGIN      
        1 gtggaccgga tccccgctgt ggcagatcga tacgctgcgc cgcctcgaaa tcccggagga
       61 catgcagaaa aagcacggct acaagccgct cggaccggct gacggaccgg tgaagaacgc
      121 aatcttcgga agcaacaatg cgcgcctctg taattacaaa ccaaagatgt cgagcgcgaa
      181 ggcggcgatc gcagcaatct gtactacggc tatattcaca agcgcgccgt gtaaaatgcg
      241 cgcaagcgcg cgtgctcgcg cgcgctgttc gtgcgctgct tgcaggtata atggcgcccg
      301 agccggcata gctcagctgg tagagcaacc gccttgtaag cggtaggtcg tctgttcgag
      361 tcagactgtc ggcaccatcg cgtcggcggt cgagtgtggc agcgccgcct ttaaatcgtc
      421 aaacccgctg cggtcgaacg gggcattttc ccgccagccg gaacttgttt gtgcggcgcg
      481 gtcaaagagc gcagcgcgcc cgcacagttc gggcttgaaa acgtgaccgc cacacctatc
      541 tagtggctgg aaaagggctg tcgggtgaac agcagaatag cggcgcgctc gtcgccccac
      601 agcgcggccc aacggaggga tcacattgat cacagtcgag catctgacga aggcgttcgg
      661 gcctaagctc gcggtaaacg atgttacatt caccgtgaac cgcggcgagg tgctgggctt
      721 tctcggtcca aacggggcgg gaaaatccac caccatgcgg atggtgaccg ggttcatccc
      781 gccgacatcg ggcagcatta cggtgggcgg tcacgacgtg ctggagaatc cgattccggc
      841 caagcgcatg attggctacc tgcctgaaaa cgcgccgggc tacgcggaca tgacggtaac
      901 cggcttcctg cgcttcgccg cggaactccg gggcctgcac ggagatgcgc gcaagcgcgc
      961 agtgaaccgg gcgatcgaac tctgctttct gcaaaacgtc gtctaccagt ccatcgacac
     1021 gctgtccaaa ggctacaagc accgcacctg cctcgcccag tccctcattc acgatccgcc
     1081 catactgatc atggacgagc cgaccgacgg tctcgacccg aatcagaagc atgaggtccg
     1141 caatctcatc aagcgcatgg ggcaggacaa ggcgatcatc ttttcgacgc atatcctgga
     1201 ggaagtcgag gcggtgtgct cacgcgtcat catcatcgat cgcggaaaga ttgtcgcaaa
     1261 cggtacgccg gaagaactca aactgcgatc cgagatggcc ggtgcggtac atctgcgcgt
     1321 gaacgacgtc ccggcggccg cactgcagga agaactgaag cgcgtgaagg gggcggcacg
     1381 ctccgaaatc ctcgcagagc gcgatggcac ggtggagttg cgggtctatc ccgacggcac
     1441 cgcggcgcga ggcagcctca cccgcgatgt cgcggcgctc gccgcgcaac agcgctggca
     1501 ggtggccgag atccacaccg aggaaggccg gctcgatgaa gtcttccgcg cgattacgct
     1561 tcccgacacg gtgagggcgg cggcatgagc ccggaagcag gggcccgatt caatggggat
     1621 gcgacgaggc gaatgctcgc cggccgccga cgcgagagcg ttgctaggcg cttgaaggag
     1681 gccgcggcat gaacgcctgg gccaacatca agacgatcat gaagcgcgag ctgggcggct
     1741 acttcacttc gcccatcgcc tatgtctttc tcgtgatctt cctgctgctc accggctttt
     1801 ttaccttcac cgtcggcagc ttcttcgagc gcggcgaagc atctctcgta tccttcttca
     1861 cctggcatcc atggctttac ctgttcctcg tgcccgcggt cggcatgcgc ctgtgggccg
     1921 aggagcgccg cctcggcacc atggagctcc tgctcaccat gccgatcacc acctggcagg
     1981 cgatcgtcgg caagttcctc gcatcgtggc tctttctggc gctggcattg gcgctcactt
     2041 ttccggtcgt catcacggtc aactacctcg gcgatccgga caacggggtc atcgtggcgg
     2101 gctacgtcgg gagcctgctc ctcgccggca cgtatctcgc gatcagttgc atgacctccg
     2161 cgctcacccg caatcaggtc atcagcttca tc
//
LOCUS       DASURG010000486         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1003483, whole genome shotgun sequence.
ACCESSION   DASURG010000486 DASURG010000000
VERSION     DASURG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003483"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1054)
                     /locus_tag="VFY80_08570"
     CDS             complement(137..1054)
                     /locus_tag="VFY80_08570"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEX6008002.1"
                     /translation="MIVSLSGCATPQVGALLAQRPAGLPERHEIADVPFYPQPMYQCG
                     PAALAMVVAHQGVPITPEAIASAVYLPGREGSLQAEMLSGARREGRVAYLLAPRLDDL
                     LREVAAGTPVVVLQNLAFSFAPLWHYAVVVGYDLAREEIVLRSGTTQRLVMTLSVFER
                     TWARSRYWAMVAMPPEHLPATAEEERYVAAVTALERVSPAAARSAYATALRRWPLDAA
                     ALLGAGNALYAMKDLHAAERAYRHATNVRPDYADAWNNLAQTLLDLGRTEEARRAIDE
                     ALERGGPRTATYRATLDAIDAQTRAASHR"
     gene            complement(1105..1485)
                     /locus_tag="VFY80_08575"
     CDS             complement(1105..1485)
                     /locus_tag="VFY80_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEX6008003.1"
                     /translation="MNFFHRCVARVLIVCMMAIGMPLPASAVIVTTDQVQAAGDRDKV
                     RSFLDRAEVRAQLENLGVDANAARARVDALTDEEVSNLAARIDTLPAGGDSFLGILFA
                     VFIILLITDILGLTKVFPFTRSVR"
     gene            complement(2115..3125)
                     /locus_tag="VFY80_08580"
     CDS             complement(2115..3125)
                     /locus_tag="VFY80_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008004.1"
                     /translation="MGHAVDLRLTAGLCAALVWELTGLAHGAASAAGNAAADYPRRPI
                     RFIEAFGSGGTTDVLSRVLGQKLTERLGQQVIVDNRPGAGGNIGAQLAAKALPDGYTL
                     FMGVVPILAAAGSMYGKLGYDVLKDFTDIGLVVSGNYVLVVGSTVPAKSVQEFIALAR
                     TRGAALRYSSSGVGSTLHLSMAMLNSMAGLKMLHVPYKGGPPMITAVAAGEVDAGAPS
                     ITLALPLIKAGRLTPLGVTGAKRAREFPNLPTIAESGVPGYELTPWYAAYAPASTPKA
                     VVNLLNSELNAILQLPDIKSGFVAQGLEAAGGTPERARQVLEVEVAKWSKVIREAGIK
                     AE"
     gene            complement(3170..>4376)
                     /locus_tag="VFY80_08585"
     CDS             complement(3170..>4376)
                     /locus_tag="VFY80_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6008005.1"
                     /translation="ATVIGCGRRTDPKTAAMLNGTAGTWLEMVEENIYAKGLPAIQVV
                     PAALAYAEHRRASGRDLLTAIVVGYEAGCRISRATKTKMAIHPSGTFGTICAAVAAAR
                     LAGANVDAMREILNVSATLGLATSRKAIIEGATVGRAYSGASGWMGILALDMVEAGFT
                     GETNGVQSVYGSIYADAPDVVSRIPQAARESFEPERVVAGLGSEYLITEGFIKMHACA
                     RSIHPALDLIEDLLAREPAGRLIPRDVERIEFTTYISPTTLRTQTPKTAFGSRFSLPF
                     AVASLIFHGRGGLANFEQPAFENPVIRELALRIDVSENPDWTALFPFKQPCEVLVRLK
                     DGSTLSARAEYTRGDPKNPRSAAEMTTKFFDVSRTVWNEDLARRIFDGIMALDRIEDV
                     RAFFLENPI"
BASE COUNT          784 a         1451 c         1379 g          762 t
ORIGIN      
        1 gcaaggagca gcttaggaaa gccgcttaac cctcagagcc atgaccgcca atttgcacaa
       61 cttgacggac acaacccggc ctgccgctac tcatcccggc ctgggctgcc gcgaacggga
      121 gcgaggagca gaagatttac cgatgtgacg cggcgcgagt ctgtgcgtcg atcgcgtcca
      181 gcgttgcgcg ataggttgca gtgcgcggcc cgccccgctc gagcgcctcg tcgattgccc
      241 gccgcgcctc ctcggtgcgg ccgagatcga gcagcgtctg ggccagattg ttccacgcat
      301 cggcgtagtc gggacgcacg ttcgttgcat gacgatacgc gcgctcggcg gcgtgcaaat
      361 ctttcatcgc gtacaacgca ttgcctgcgc caagcaatgc ggccgcatcg agcggccatc
      421 gccgtaacgc cgtcgcgtaa gcgctacgcg ccgcggcggg tgacacacgc tccaatgccg
      481 tgacagcggc gacgtaacgc tcctcttccg cggtagcggg cagatgctcc ggaggcatgg
      541 caaccatggc ccaatacctg ctgcgcgccc atgtgcgctc gaacacgctg agtgtcatga
      601 cgaggcgctg cgtagttccc gaccgcagca cgatttcctc gcgcgcgagg tcgtagccca
      661 cgacgaccgc gtaatgccag agcggcgcga agctgaacgc gagattctgc agaacgacga
      721 ccggcgtgcc ggccgcgacc tcgcgcagca gatcatccag ccttggagcg agcaggtagg
      781 cgacgcgccc ttcgcgccgc gcgccgctca gcatctccgc ctgcagggag ccttcgcgtc
      841 ccggcagata cacggcggat gctatggcct ccggagtgat cggcacgccc tggtgcgcaa
      901 ctaccatcgc gagcgcggcg gggccgcatt ggtacatggg ctgcgggtag aacggcacat
      961 cggcgatctc gtgccgctcg ggcagaccgg ccgggcgctg cgccagcagc gcgccgacct
     1021 gcggtgttgc acaacccgac agcgatacga tcaaaaagag aaatcccgcc agggcggggc
     1081 gtcgtttgca gcaaaaggtc aatgtcagcg caccgacctc gtgaacggga ataccttcgt
     1141 gaggccgaga atgtcggtga tgagcaggat gatgaagacg gcgaacagga taccgagaaa
     1201 gctgtcgccg ccggcgggca gcgtgtctat gcgcgcggcg agattgctca cttcttcgtc
     1261 ggtcagcgca tcgacgcggg cgcgggctgc gttggcatcg acgcccaggt tttccaactg
     1321 cgcgcgcact tccgcgcggt cgaggaagct tctcaccttg tcgcgatcgc cggcggcctg
     1381 cacctgatcc gtggtgacga taaccgctga ggcaggcagc ggcatcccga ttgccatcat
     1441 gcatacgatg agtacgcggg ccacgcaacg atggaaaaaa ttcatggtgt ctccttggtg
     1501 atggacatgc gtgcactgca tatgtggcgg tggtgaagca ttatccatac gcgcgctttt
     1561 tcgcgtcaaa gcgacgacgt atcgagcgtg attttatccg cacctgcaca tgacgcaggc
     1621 atttcttgtc ttcgccatgc cgcgcactgt cgtcatttgc agacacagcg tccgcgcgtg
     1681 acgggctcgt agcccgggat acgccgccgg gtcgggatca tgggcgcgag cggtgagggc
     1741 tgggcgctcg taatgacgag cgcgtaatcg acccgccttg atgtggccgg ctgccgatcc
     1801 gatcgcgaaa ggcctcaatg aatccatggc ggcgccggga cccgacgccg tctcgcacgt
     1861 gccagggacg ctggtcggca accaccaatg ccgacgttga tcgacacctt gaaagttgcg
     1921 aaggagaagc ggctggcggc tcaaggccga cagtcgcaat gacccacacc gaggagaaag
     1981 ggccgttcca gatgatgtat tgagtgcgga aaatttccga cccgctggtg taggtgcttt
     2041 acccagcgcc gcaaccttcg tcgacgcgat cacaatagct cacatggcat gcgctcgttt
     2101 tcgcgttcgt tggttcactc cgccttgatt ccggcttccc tgatcacctt cgaccatttt
     2161 gcgacttcga cctccagcac ttggcgcgcg cgctcgggcg taccgcctgc cgcctccagc
     2221 ccctgcgcca cgaagcccga cttgatgtcc ggcaactgca ggatggcgtt cagctccgag
     2281 ttgaggaggt tgaccactgc cttcggtgtg ctggccggcg catatgctgc ataccacggc
     2341 gtcagctcgt aaccgggcac gcccgactcc gcgatggtag gcaggttggg aaattcgcga
     2401 gcgcgcttcg cgccggttac gccaagcggc gtcagccgtc ccgccttgat gagcggaagc
     2461 gcaagcgtga tgctgggcgc gccggcatcc acttcgccgg ccgcgactgc ggtgatcatg
     2521 ggcggaccgc ccttgtaggg tacgtgcagc atcttcaggc ccgccatgct attcaacatc
     2581 gccatcgaaa gatgcagcgt gctgcccacg ccggacgagc tgtagcgcag cgctgcgccg
     2641 cgcgtgcggg cgagcgcgat gaactcctgc accgacttcg ccggcaccgt ggagcccacc
     2701 accagcacat agttgccaga aaccacgaga ccgatatccg tgaaatcctt cagcacgtca
     2761 tagccaagct ttccgtacat gctgccagcc gctgcaagaa tcggcaccac gcccatgaag
     2821 agcgtgtagc cgtcgggaag agccttcgcg gcaagctgcg ctccgatgtt gcccccggcg
     2881 cccgggcgat tgtccacgat cacctgctgt ccgaggcgct ccgtgagctt ctgcccgagt
     2941 acccgcgaca gcacgtcggt ggtgcccccg gatccgaacg cctcgatgaa gcggatcggg
     3001 cggcgcggat aatccgccgc agcatttccg gcagcgctcg ctgccccgtg cgcgagcccg
     3061 gttagctccc acacgagggc cgcacacagc cccgccgtta gtcgcaagtc gaccgcgtga
     3121 cccatagacc ctccagctca attgcccccg tcaccggtca cgtcctgcat cagatcggat
     3181 tttcgaggaa gaaggcgcgc acgtcctcga tcctgtcgag cgccatgatg ccgtcgaaga
     3241 ttctccgggc cagatcttcg ttccacaccg tgcgcgacac gtcgaagaat ttcgttgtca
     3301 tctccgcggc gctgcgcggg ttcttcgggt cgccgcgcgt gtattccgcc cgggcgctga
     3361 gcgtgctgcc gtccttcaac cgcaccagca cctcgcacgg ctgcttgaac gggaacagcg
     3421 ccgtccagtc cggattctcc gaaacgtcga tacgcagcgc gagctcgcgg atgaccggat
     3481 tctcgaacgc cggctgctcg aagttcgcga gcccgccgcg cccgtggaaa atgagcgacg
     3541 ccacggcaaa gggcagcgaa aatcgcgatc caaacgccgt cttcggcgtt tgcgttcgca
     3601 gagtcgtcgg cgaaatgtag gtcgtgaatt cgatgcgctc gacgtcccgg ggaatgagcc
     3661 gcccagccgg ctcgcgcgcc aggagatcct cgatcaggtc gagcgcgggg tggatcgagc
     3721 gcgcgcaggc atgcatcttg atgaagccct cggtgatcag gtactccgag ccgaggccgg
     3781 caacgacgcg ctcgggctca aaggactcgc gcgccgcctg cggaatgcgg ctgacgacat
     3841 ccggcgcatc ggcgtagatc gatccgtaaa ccgactgcac gccattggtc tcgcccgtaa
     3901 atcccgcttc gaccatgtcg agcgcaagga tgcccatcca gccgctcgcg ccgctgtaag
     3961 cgcggccaac agtggcgccc tcgataatcg ccttgcggct ggttgcgagc ccgagcgttg
     4021 cgctcacgtt caggatttct cgcatggcat cgacgttagc gccggcgagc cgtgcggcgg
     4081 ccaccgcggc gcagatcgtc ccgaacgtgc cgctcgggtg gatcgccatc ttcgtcttcg
     4141 tcgcgcggct tatacgacag ccggcttcgt acccgactac gatcgccgtc agcagatcgc
     4201 gtcccgaagc gcggcggtgc tcggcgtacg caagcgcggc cggaacgacc tggatggccg
     4261 gcagcccttt ggcatagatg ttttcttcca ccatctcgag ccacgtgccc gcggtcccgt
     4321 tcagcatggc ggcagtcttg ggatcggtcc tgcgtccgca accgattacc gtcgca
//
LOCUS       DASURG010000487         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_289811, whole genome shotgun sequence.
ACCESSION   DASURG010000487 DASURG010000000
VERSION     DASURG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289811"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VFY80_08590"
     CDS             <1..1639
                     /locus_tag="VFY80_08590"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX6008006.1"
                     /translation="PIFHVNGDDPDAVCLVTEIALDYRMKFHRDVVVDLVCYRRLGHN
                     EQDEPMVTQPLMYKKIQKLPTTRKRYVDRLAKEGVISEAEAEEMIANCRNALDSGYHT
                     NKTILSRYKPPFEVDWKPYIGTRWNEKDDTRLPIETLRVLGERITQVPENFKLHPRVQ
                     KIIEDRRLMAQGKIPLDWGMAEHLAYATLLNEGYSIRISGQDSARGTFFHRHAVLHDQ
                     NRERWDQGTYIPLQNVSAKQGDFVVIDSLLSEEAVVGFEYGYATSGPNELVVWEAQFG
                     DFANGAQVVIDQFIASGEVKWGRLCGLTMLLPHGYEGQGPEHSSARIERYLQLCADYN
                     MQVCIPATPVQFYYLLRRQMVRPYRKPLIIFTPKSLLRHKESVSPLEEFANDKFRPVN
                     EDWDAAEINPQNVKRVIFCSGKVYYDLRAARREREIRDLPLVRIAQLYPFPHDDFRAQ
                     IERYKGADEIVWCQEEPRNQGAWRHIQHYLLRHLLPHQKLFYAGRESSASPAAGYKQL
                     HDEQQKELVGMALTMSAGSGPRILSLQPRPLSAGEEN"
     gene            1649..>2027
                     /locus_tag="VFY80_08595"
     CDS             1649..>2027
                     /locus_tag="VFY80_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6008007.1"
                     /translation="MLIDVKVPQLSESVAEATLVTWHKKQGDYVKRDENLVDIETDKV
                     VLETPAPDSGVLAAIIKSDGGTVTSNEVIARIDTEAKPAAAAEPVPPTQAQETRQPAK
                     PAAAGVGALPAAQKLATEKKMDTA"
BASE COUNT          419 a          641 c          647 g          320 t
ORIGIN      
        1 cccgatcttt catgtgaacg gcgacgatcc cgacgcggtg tgcctcgtca cggagatcgc
       61 gctcgactac cgcatgaaat tccaccgcga cgtcgtggtc gatctcgtgt gctaccggcg
      121 cctcgggcac aacgagcagg acgagccgat ggtgacgcag ccactcatgt acaagaagat
      181 ccagaagctg ccgaccacgc gcaagcgcta cgtcgaccgg ctcgcaaagg aaggtgtcat
      241 ttccgaggcg gaagcggaag agatgatcgc gaattgccgc aacgcgctcg actcgggcta
      301 tcacaccaac aagacgatcc tctccaggta caagccgccg ttcgaggtag actggaaacc
      361 ctacatcggt acccgctgga acgagaagga cgacacgcgg cttcccatcg aaacgctcag
      421 agtgctgggc gagcgcatca cgcaggtgcc ggagaacttc aagctccatc cacgggtgca
      481 gaagatcatc gaagaccggc gtctcatggc ccagggaaag atcccgctcg actggggcat
      541 ggctgagcat ctcgcgtatg cgacgctgct gaacgagggc tattcgattc gcatttcggg
      601 ccaggactcc gcccgcggga cgttcttcca ccgccacgcc gtgctgcacg atcagaaccg
      661 cgagcgctgg gatcagggca cctacattcc gctgcagaac gtgagcgcga agcagggtga
      721 cttcgtggtg atcgattcgc tgctctcgga agaggcggtg gtcggcttcg aatacggcta
      781 cgcgacttcg ggtcccaacg agcttgtggt ctgggaggcg cagttcggcg acttcgcgaa
      841 cggcgcccag gtcgtcatcg atcagttcat cgcatccgga gaggtgaagt ggggccggct
      901 gtgcggcctc acgatgctgc tcccgcacgg ttatgagggg caggggcccg agcactcctc
      961 ggcgcgcatc gagcgctatc tgcagctttg cgcggactac aacatgcagg tctgcattcc
     1021 ggcgacgccc gtgcagttct actatctgct gcgccgccag atggtgcggc cgtaccgcaa
     1081 gccgctcatc atcttcacgc caaaaagcct gctgcgccac aaggagtccg tctctcccct
     1141 ggaggaattt gcgaacgaca aattcaggcc ggtgaacgaa gactgggatg cggcggagat
     1201 caacccgcag aacgtgaaac gcgtgatctt ctgcagcggc aaggtctact acgacctgcg
     1261 tgccgcgcgc cgcgagcgcg agatacggga tctgccgctg gtgcgcattg cgcagctcta
     1321 tccgtttccg cacgacgatt tccgcgccca gatcgagcgt tacaagggcg cagacgaaat
     1381 cgtgtggtgc caggaagagc cacgcaacca gggcgcgtgg cgccacatcc agcactatct
     1441 gctgcgccat ctcctgccac atcagaagct cttctatgcg gggcgcgagt cctcggcttc
     1501 gccggccgcg gggtacaagc agctccacga tgagcagcag aaggagctag tcggcatggc
     1561 gctcacgatg tccgccggca gcgggccgcg gatcctgtcg ctgcagccgc gtccgctgtc
     1621 ggcgggcgag gaaaactaaa ggcggccgat gctgatcgac gtcaaggttc cgcaactctc
     1681 cgagtcggtc gccgaggcga cgctcgtcac ctggcacaag aagcagggcg actacgtgaa
     1741 gcgtgacgag aacctcgtcg atatcgagac cgacaaggtg gtgctggaga cgcccgcgcc
     1801 cgactcgggc gtgctcgcag ccatcatcaa aagtgacggc gggacggtga ccagcaacga
     1861 agtgatcgcg cgcatcgata ccgaagcgaa gcctgctgcc gccgcggagc cggtgccgcc
     1921 cacgcaggcg caggaaacac gacagccggc gaagcccgcc gcggcgggcg tgggcgcact
     1981 tcccgcggca cagaagctcg ccaccgagaa gaaaatggac accgccg
//
LOCUS       DASURG010000488         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1003560, whole genome shotgun sequence.
ACCESSION   DASURG010000488 DASURG010000000
VERSION     DASURG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003560"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1386)
                     /locus_tag="VFY80_08600"
     CDS             complement(73..1386)
                     /locus_tag="VFY80_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6008008.1"
                     /translation="MRRVSASFIIVAPWILTVLFSVLGARLSWQLGVLAFLFSAFVAG
                     AVARYLGHATRELRRELRSGQITQTEPHRIIEIDALRAAVRVASRESRQSAEQTVRTE
                     EQLLAVIESVTEGILQISEHARIMRLNPAARELLNLPANAVGQTVPALIRHPELRYCI
                     EQAATGQSFEPVEILFDERQLLVLPRPLRAFPGAVIAIVNLTEVRRLESVRRDFVANV
                     SHELKTPLTSIRGYTETLLNEELPPEVRRQFLDVVHKNADRLQRIVDDLLDLSRLQSG
                     GWQPELEQVDPVSLAEDVWSSCVGAQRKNIRFNVHANTARHVLADAGGLRQVLSNLFD
                     NAIRYTPEGGEVTVSVRNGNGVNTDENYIELAVHDTGTGISRDALPRIFERFYRVDPA
                     RSRQEGGTGLGLSIVKHLVERMDGAVSAESELGKGTTIRIKLPAA"
     gene            complement(1389..>2074)
                     /locus_tag="VFY80_08605"
     CDS             complement(1389..>2074)
                     /locus_tag="VFY80_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6008009.1"
                     /translation="VVEDERDIAALVAYHLTREGYRVRTAEGGNEALAAVSADRPDLI
                     VLDLMLPGFSGYEVLAEIRRRPELAEVPIIVLTARREESDRIKGLELGADDYLTKPFS
                     PRELVLRVGAVLRRAQSLPVAGSGRTLRGGNIVVDLNALEVTVQGAPVELTPTEYRLL
                     VALLERRGRVQNRQQLLEAAWDVHARIETRTVDMHVQRLRNKLGPGGEWIETVRGFGY
                     RFRGKDDEE"
BASE COUNT          391 a          668 c          629 g          386 t
ORIGIN      
        1 caggacgatc gtgagaccga tgccgatgat gaccggcagt attcttcgag cgacagagtc
       61 agacatcaac cactatgctg ccggcagttt gatgcgaatg gtcgtgcctt tgcccaattc
      121 gctttccgcg ctcaccgcac catccatgcg ttccaccaaa tgtttgacga tggacaagcc
      181 caaaccggtc ccgccttcct ggcgcgagcg cgccggatca acacgataga atcgttcgaa
      241 gatgcgcggc aacgcgtcgc gggaaatacc cgtacccgtg tcgtgcacgg ccagttcaat
      301 gtagttctcg tccgtattta cgccattgcc gttgcgcacg gagaccgtga cttcgccgcc
      361 ctcaggcgtg taacggatgg cgttgtcgaa cagattcgat aatacctgcc gtagtccgcc
      421 ggcgtcggcc agcacatggc gggccgtgtt cgcgtgcacg ttgaaccgaa tgttcttgcg
      481 ctgtgctcca acgcagctgc tccagacatc ttccgccagg ctgacgggat cgacttgctc
      541 cagctcgggt tgccagccgc ccgactgcaa gcgggaaagg tcgagcagat catcgacgat
      601 gcgctgcagg cgatcggcat tcttatggac cacgtccagg aactgtctgc gcacttcggg
      661 tggcagttct tcgttgagca gggtttcggt gtaaccgcga atcgaggtca ggggcgtttt
      721 cagctcatgg ctgacgttcg ccacgaaatc gcggcgcacg ctttccagtc gccgtacttc
      781 cgtcaggttc acgatggcga ttaccgctcc ggggaacgca cggagcggtc gcggcagaac
      841 cagcagctgg cgctcgtcga agagaatttc cacgggctcg aacgattgtc ccgtggcggc
      901 ctgctcgatg caataccgca actctggatg ccggatcaga gcaggaacag tctgccccac
      961 ggcattcgcg ggcagattga gtaattcgcg ggcggctgga ttcagccgca tgatgcgggc
     1021 gtgttcactg atctggagga taccttcggt gacggattcg atgacggcca gcaactgctc
     1081 ttcggtccgg accgtttgct ccgcgctttg cctgctctca cgcgacgcca cacgcacggc
     1141 ggcgcgcagc gcatcgatct cgatgatgcg gtgcggctcg gtctgagtga tttgtccgga
     1201 acgcagctcg cgacgcagct cccgggtggc gtgtccgagg tagcgcgcca ccgcccccgc
     1261 gacgaacgcg gagaatagaa aggccagcac gcccagctgc cagctgaggc gcgcgcccag
     1321 cacgctgaac agcaccgtca ggatccacgg agctacaatg atgaagctgg cgctaacgcg
     1381 ccgcatgctc actcctcgtc gtctttacct cgaaaccggt agccgaagcc gcgcaccgtc
     1441 tcaatccact caccgccggg accgagcttg ttgcgcaatc gctgaacatg catgtcgacc
     1501 gtgcgcgtct cgatgcgcgc gtgtacatcc caggcggcct ccagcagctg ctgtcgattc
     1561 tgcacgcgac cgcgccgctc gagtagcgcg accagcaatc ggtactcggt cggcgtgagc
     1621 tcgaccggtg cgccctgaac ggttacctcg agtgcattca gatcgacaac gatattgccg
     1681 ccgcgcagtg tacgtcccga gcccgccacc ggcagcgatt gcgcacgccg caggaccgca
     1741 cccacgcgca agaccagctc gcgcgggctg aatggcttgg tcagatagtc atcggcaccc
     1801 agctcgaggc ccttgatgcg atccgactcc tcccggcgtg cagtcaggac gatgatcggc
     1861 acttccgcca gttccgggcg acgtcgaatt tcggccaaca cttcatagcc ggaaaagccc
     1921 ggcagcatga gatcgagaac gatcaggtcg ggacgatcgg cggagaccgc cgcaagggct
     1981 tcgttgccgc cttccgccgt gcggacgcgg tagccttccc gggtgaggtg atacgcgacc
     2041 agggcagcga tatcacgctc gtcttctacg acca
//
LOCUS       DASURG010000489         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_289892, whole genome shotgun sequence.
ACCESSION   DASURG010000489 DASURG010000000
VERSION     DASURG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289892"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFY80_08610"
     CDS             complement(<1..228)
                     /locus_tag="VFY80_08610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6008010.1"
                     /translation="MAEIRNYTVNFGPQHPAAHGVLRLVLELDGEVVQRADPHIGLLH
                     RATEKLAEDRTYLQNLPYFDRFDYTTMLSNEH"
     gene            complement(325..1077)
                     /locus_tag="VFY80_08615"
     CDS             complement(325..1077)
                     /locus_tag="VFY80_08615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6008011.1"
                     /translation="MPSRLETLVANLGSVLGDKLAGAVTALNEVTVVVKPEDLLDTAT
                     RLRDAPELRFEQLIDVCGVDYSTYGKPASETEGDQLDTDSPAGGAPSEAGAAPAIDGM
                     APERGVAAAMRRARWPLAGRRYAAVYHLLSLAQNWRLRVRVFAADEEFPVVPSVIGIW
                     PSANWFEREAFDLFGIVFEGHPDLRRILTDYGFVGHPFRKDFPTSGTVEMRYDPDQQR
                     VIYQPVTIEPRQNVPRIVREENYADSEGFRKG"
     gene            complement(1078..1557)
                     /locus_tag="VFY80_08620"
     CDS             complement(1078..1557)
                     /locus_tag="VFY80_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX6008012.1"
                     /translation="MGAPENLSDKGFITTTADNLINWTRTGSLWPVTFGLACCAIEMM
                     HAGASRYDLDRFGIVFRPSPRQSDVMVVAGTLCNKMAPALRKVYDQMAEPRWVISMGS
                     CANGGGYYHYSYSVVRGCDRIIPVDIYVPGCPPTAEALLYGILQLQNKIRRTNTIAR"
     gene            complement(1755..2453)
                     /locus_tag="VFY80_08625"
     CDS             complement(1755..2453)
                     /locus_tag="VFY80_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008013.1"
                     /translation="MHEARPEQPSFPAMGRSRALARLNGRILAAYSRRTVVALPHGLP
                     VRVLLGRLEPVLVLNVEKEMRKDAIVIARAGDAHGAAPRAELVRELVEATKAIDREFL
                     AHARTFPVRLNIRYEDIVPVRTRRIERLYGGARRILQAWSADQTLRGALRVSFPEAEL
                     EGFLRELLQLYAQEVRVLSRSVALPALLTPLREHLARGLHAAMSDIAVALARELAQGL
                     HHAAPNARGISSAS"
BASE COUNT          442 a          827 c          825 g          445 t
ORIGIN      
        1 atgctcgttc gagagcatgg tggtgtagtc gaagcggtcg aaatacggca ggttctgcag
       61 gtaggtccgg tcctcggcga gcttctcggt cgcccgatgc aggagaccga tgtgcggatc
      121 cgcgcgctgg acgacctcgc cgtcgagctc caataccagg cgcagcacgc cgtgcgccgc
      181 ggggtgctgc ggcccgaagt tgacggtgta gtttctaatc tctgccatag tcgttccaga
      241 ctggcgtgcg cggagcgctc aaggcgtccc gcgcacgccc ggtgagggcg accgcgcacg
      301 ctgcgcctgg ctgaacccgc ggaatcatcc cttgcggaat ccctcgctgt ccgcgtagtt
      361 ctcctcgcgc acgatgcgtg gcacgttctg ccgcggctcg atcgtgaccg gctggtagat
      421 cacgcgctgc tgatcggggt cgtagcgcat ctccaccgtg cccgacgtgg ggaaatcctt
      481 gcggaacggg tgacccacga aaccatagtc tgtgaggatg cggcgcaggt cagggtgtcc
      541 ctcgaacacg atgccgaaaa gatcgaaggc ttcgcgctcg aaccagtttg ccgacggcca
      601 gatgccgatc acggagggca cgacgggaaa ctcttcgtcg gccgcgaaaa cgcgcacgcg
      661 caagcgccag ttctgcgcga gcgacagcag gtggtagacc gccgcgtagc ggcgtcccgc
      721 gagtggccag cgcgcgcgcc gcatcgcggc ggcgacaccg cgctccggcg ccatcccgtc
      781 gatcgccggc gccgcccccg cttcggacgg cgcgccgccg gcgggcgagt cggtatcgag
      841 ctgatcgccc tcggtctccg aggcaggctt gccgtaggtg ctgtagtcga cgccgcatac
      901 gtcgatcaac tgctcgaacc tcaattcggg cgcatcgcgc aggcgggttg ccgtgtctaa
      961 gaggtcctcg ggcttcacca cgaccgtgac ctcgttcagc gccgtcacgg cgccagcgag
     1021 cttgtcgccg agtaccgagc ccaagttggc gacgagggtt tcgagacggg atggcatcta
     1081 acgcgcgatg gtattggtgc gtctgatctt gttctgaagt tggaggatgc cgtaaagcag
     1141 cgcctccgcg gtgggcgggc agcccggtac gtagatgtcg accggtatga tgcgatcgca
     1201 gccgcgaacg acagaatacg aatagtggta gtagccaccg ccgttcgcgc acgagcccat
     1261 ggaaatcacc cagcgaggct cggccatctg gtcatagacc ttgcgcagcg cgggcgccat
     1321 cttgttgcac agagtgcctg ccacgaccat cacgtccgat tgccgcgggc ttgggcggaa
     1381 cacgatgccg aagcgatcga ggtcgtagcg ggaggcgccc gcgtgcatca tctcgatcgc
     1441 gcagcacgcg aggccgaagg tcaccggcca cagcgaaccg gtccgggtcc agttgatgag
     1501 gttgtccgcg gtggtggtga tgaaaccctt gtcggagaga ttttcgggag cacccatgtg
     1561 ttagctctca gttcaggacc ggtgggtcgt caagcgcttt gcttcatgcc gtgtcgggca
     1621 gccgtagtct gcgctgccga acgcaaaagt tgcgacccgg tggacgagta ccggttctgc
     1681 gtaaagacgt gaaattttaa ccacggcgcg caggcaggtc aacgcaaaag agttccacag
     1741 atcaaaaagt tgcgtcatga tgcggaggaa attccgcgcg cgttcggcgc agcgtggtgc
     1801 aggccttgcg cgagttcgcg cgcaagcgcg accgcaatgt cgctcatggc cgcgtgcagt
     1861 ccccgcgcca ggtgttcgcg caacggcgtg agcagcgccg gaagcgccac ggaccggctc
     1921 agcacgcgca cttcctgcgc gtagagttgc agcagttcac gcaggaatcc ttccagttct
     1981 gcttccggga aactgacgcg caacgccccg cgcaacgtct gatctgcgct ccacgcctgc
     2041 agtattctgc gggcaccccc gtacagccgc tcgatgcgcc tcgtgcgcac aggcacgatg
     2101 tcctcatagc gtatgttgag gcgcaccgga aaggtacgcg cgtgcgccag aaattcacga
     2161 tcgatcgcct tcgttgcctc gaccaactcg cgcaccagtt ccgcgcgggg tgccgcgcca
     2221 tgcgcatcgc ccgcgcgcgc gatgacgatg gcatccttgc gcatttcctt ctccacattc
     2281 agcacgagca ccggctccag ccggccgagc agcacccgca ccggcaaacc gtgtggcagc
     2341 gccacgacgg ttctgcggct gtatgccgca aggatcctgc cgttcagcct tgcgagcgcc
     2401 ctgctccggc ccatcgccgg gaaagacggt tgctccgggc gcgcctcatg cacccctcgg
     2461 cttctccgcc ttgtgcagcc cgatcatgcc ggcgaagatc gtcttagccc gcacgttgca
     2521 gtacccagtg tccgccaat
//
LOCUS       DASURG010000490         4133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_492840, whole genome shotgun sequence.
ACCESSION   DASURG010000490 DASURG010000000
VERSION     DASURG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492840"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFY80_08630"
     CDS             complement(<1..329)
                     /locus_tag="VFY80_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX6008014.1"
                     /translation="MTTAFVTHSDCLLHDMGAHHPEQPARLKAVEDQLIASGLIDHLR
                     YVEAPLVPREDLERVHTKRYIDALEDAAPVTGLTHLDPDTAMNPHTLAAARRAAGAGI
                     AATDMVIR"
     gene            379..942
                     /locus_tag="VFY80_08635"
     CDS             379..942
                     /locus_tag="VFY80_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6008015.1"
                     /translation="MTTEIASSGGRMPQRRPYPAHLVRSATLADGATIVIRPICPDDA
                     KSEQEFVRALSPESRYFRFMEALRELSPQMLSHFTQIDYDRHMALVAVTPGGERQIGV
                     ARYIVTSDEASCEFAIVIADDWQQRGVATVLMDALMDAARARGTRRMFGDVLAANHRM
                     LELMHRLGFRSVRHSDDPGLLRVEVNL"
     gene            1020..1925
                     /locus_tag="VFY80_08640"
     CDS             1020..1925
                     /locus_tag="VFY80_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6008016.1"
                     /translation="MDRIVAQVGGIVLGKEQQIRLALSCLLARGHLLIEDIPGVGKTM
                     LAHALATSLGLGFQRIQFTSDLLPADILGISIYDREAHAFQFHPGPIFTQLVLADEVN
                     RATPKAQSALLEAMEEHQVTIEGETRRLPHPFFVIATQNPSTQVGTFPLPESQLDRFL
                     MCIELGYPDASAERELLRGEDRRDLLDTLTPCIEVKTLIELQKRARGVHTSAALLDYV
                     QAIVGHTRRAGDYPNGLSPRAALALVQAARAWALMAGREHVVPEDVQAVLTGVVGHRL
                     TVAGALGRVNRADLVKHLIERVPIP"
     gene            1993..2955
                     /locus_tag="VFY80_08645"
     CDS             1993..2955
                     /locus_tag="VFY80_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6008017.1"
                     /translation="MYTALRNALYAWLFQLRGPQSGPIVLVQRRIFIVPSAQGFLFAG
                     VLLLMLIGSINYALSLGFILTFLLTALGLNAMVYTFRNLARLQISAGRAHPVFVGDTA
                     EFTLHIENPTRADRYAIGITADKLAATYVDVPAGRCVSAGVRIPARRRGRMRPGRLTL
                     FTRYPLGLYYAWSYVELEAYCLVYPRPSAERLPLPASEPADGTGTQRGSGLEDFSGMR
                     QYHPGDSPRHIAWKAAARGEELLTKQFSGRADAELWLDWTHLPATCGTEERLSYLARW
                     VIDAHTSGVAYGLRIPGRTLAPASGDAQRDRCLQALALFGQEDE"
     gene            2948..>4133
                     /locus_tag="VFY80_08650"
     CDS             2948..>4133
                     /locus_tag="VFY80_08650"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HEX6008018.1"
                     /translation="MNSISGPAVDFRNMLWLTAALALVAAPHVQRLPLWVMLFAAALA
                     LWRLYLGSTNHPLPRKTLIMVVVLVATAAVFLNYRTLFGRDAGVSLLVVMLALKLLET
                     ESRRDALLLILLAYFLIVTNFLYSQTIPTALYMLGCLWIVTAAMIACNYARSRPDYRT
                     PLRSAALMLAQAAPLMLALFLLFPRATGPLWRLPQDAHGGVSGLSDTMTPGSVARLTL
                     SDDVAFRVEFKSSIPPARSMYWRGPVLWDFDGRTWTMPRSFPQANPKFESLSPAVEYT
                     VTVEPHNRLWLFGLDLPSAAPQGAIVSRDFQLRSVAPVVNRTRYEMQSYLEYRTGLDE
                     RRNALERALQLPESANPRTVRFAKELRNRHSTDAALMREVLLMFRNENFHYTLEPPPL
                     GPH"
BASE COUNT          657 a         1391 c         1292 g          793 t
ORIGIN      
        1 cgtatcacca tgtcggtcgc cgcgatgccg gcgcccgctg cccggcgcgc cgcggcgagg
       61 gtgtgcggat tcattgcggt atcggggtcg agatgggtaa gacccgtcac cggcgccgca
      121 tcttcgagtg catcgatgta gcgcttcgtg tgcacgcgct cgaggtcttc gcgcggcacg
      181 agcggcgcct cgacatagcg caggtgatcg ataaggcccg aggcgatcaa ctggtcctcg
      241 accgccttga gccgtgccgg ctgctcgggg tggtgagccc ccatgtcgtg cagcagacag
      301 tccgaatggg tgacgaaggc agtggtcaat tgttcacact ccgatgctgc tccggcatct
      361 tataatgcat caatcaaagt gaccaccgag atcgcatcct cgggcggaag gatgccgcag
      421 cgccggccct atcccgccca cctcgtgcgc agtgcaacgc tcgctgacgg agccaccatc
      481 gtgattcgac ccatctgccc ggacgacgca aagagcgagc aggagttcgt gcgcgcgctc
      541 tcgcccgaat cgcgctactt ccgcttcatg gaagcgctgc gcgagctttc tccgcagatg
      601 ctctctcatt tcacccaaat cgattatgac cgccacatgg cgctcgtcgc ggtgacgccg
      661 ggcggcgagc gccagatcgg agtggcacgc tatatcgtga cgtccgacga ggcgagctgc
      721 gagtttgcca tcgtcattgc cgacgactgg cagcagcgcg gcgtggcgac cgtcctgatg
      781 gacgcgctga tggacgcggc ccgcgcgcgg ggcacgaggc ggatgtttgg cgacgtgctc
      841 gccgccaatc accgcatgct ggaactcatg caccggctcg gattccgttc cgtgcgccat
      901 tcggatgacc ccgggctgct gcgcgtcgag gtcaatttgt gaggatgacc gtgccggata
      961 ccgcggccaa tccgaatgtt catccgccag gcgagcgcgc tgcggcctgg accgatggca
     1021 tcgatcggat tgtcgcgcag gtgggcggca ttgtcctcgg caaggagcaa cagatccgtc
     1081 tcgcgctttc gtgcctgctc gcgcgaggtc atctgctgat cgaggatatc ccgggtgtgg
     1141 gcaagacgat gctcgcgcat gcgctcgcca cgtccctcgg gctcggattt caacgcatcc
     1201 agttcaccag cgatctcctg ccggcggaca tccttgggat ctcgatatac gatcgcgagg
     1261 cgcatgcgtt tcagttccat cccggtccga tcttcacgca gctcgtgctg gccgacgagg
     1321 tgaaccgggc gacgcccaag gcacagagcg cgttgctcga ggcgatggag gaacaccagg
     1381 ttacaatcga gggcgaaacg cgccggctgc cacatccctt cttcgtcatc gccacgcaga
     1441 acccctccac ccaggtggga acgtttccgc tgcccgagtc tcagctcgac cgctttctca
     1501 tgtgcatcga gctcggctat cccgatgcaa gcgcggaacg ggaactgctg cgcggtgaag
     1561 atcgccgcga cctcctggat acgctcacgc cgtgcatcga ggtgaagacg ctgatcgagc
     1621 tgcagaagcg ggcgcgcggg gtgcacacct cggccgcgct gctcgattac gtccaggcga
     1681 tcgtggggca tacccgacgc gcaggcgatt atccaaacgg gctctcgccg cgggcggcgc
     1741 ttgcgctcgt tcaggccgcc cgtgcctggg cgctcatggc ggggcgcgag catgtggtgc
     1801 cggaggacgt gcaggcggta ttgactggcg tggttggcca ccggttgacc gtcgccggcg
     1861 ctttggggcg cgtcaatcgc gccgacctgg tgaagcattt gatcgagcgc gtgcccatcc
     1921 cgtgacgctc atccacccca tgcgcccgca aggcgacctg caggttttgc ggtacggcac
     1981 ccgtagttga acatgtacac cgcgcttcgc aacgcgctct atgcctggct cttccagttg
     2041 cgcgggccgc agagcgggcc gatcgtgctc gtccagcggc gcatcttcat cgttccttcg
     2101 gcgcaagggt ttctcttcgc cggcgtgctt ctcctgatgc tgatcggctc gatcaactac
     2161 gcactcagcc tcggctttat cctgacgttc ctgctcacgg cgctcggcct gaatgccatg
     2221 gtgtacacgt ttcgcaatct ggcccgcctg caaatttccg ccggccgtgc ccatcccgta
     2281 ttcgtcggag ataccgcgga atttactctt catatcgaga atccgacccg ggctgaccgc
     2341 tatgcgatcg gcatcaccgc cgacaagctt gccgccacgt acgtcgatgt gccggcgggc
     2401 cggtgtgtga gcgcgggcgt gcgtatcccg gcccggcgcc gcgggcggat gcgccccggc
     2461 cggctcacgc tctttacgcg ctatccgctg ggactctatt acgcctggtc ctacgtcgag
     2521 ctcgaggcct actgcctcgt ctatccccga ccttcagccg agcgcttgcc gcttcccgct
     2581 tccgagccgg cagacggcac cggcacgcag cgcggctcag gactcgagga tttcagcggg
     2641 atgcggcagt accatccggg agattcgccg cgtcacatag catggaaagc cgcggcgcgc
     2701 ggcgaagagc ttctgaccaa gcagttcagc ggccgcgccg atgccgaact gtggctcgac
     2761 tggacacacc tgccggccac ctgcggtacg gaggagcggc tgtcgtatct cgcacgctgg
     2821 gtgatcgatg cacatacatc gggcgttgca tacggcttgc gcatcccggg tcggacactg
     2881 gcgcccgcga gcggagacgc ccagcgcgac cgctgcctgc aggcgctcgc gctgttcggg
     2941 caggaagatg aatagcataa gcgggcccgc cgtcgatttt cgcaacatgc tgtggctcac
     3001 ggctgcgctc gcgctggtcg cggcgccgca cgtgcagcgc ttgccgctct gggtcatgct
     3061 gttcgccgcc gcactggcgc tctggcgact ctacctcggc agcacgaacc atccgctgcc
     3121 tcgcaagacg ctcatcatgg tggttgtgtt ggtcgccacg gcggccgttt ttctcaacta
     3181 tcgcacgctc ttcgggcggg atgccggcgt ctctctgctc gtcgtgatgc tggcgctgaa
     3241 gctgctcgag accgagtcga ggcgcgatgc gctgctgctc atcctgcttg catactttct
     3301 catcgtcacc aatttcctgt actcccagac gattccgacc gcgctctaca tgttgggctg
     3361 cctgtggatc gtcaccgccg ccatgatcgc atgcaattac gcccggtcgc ggcccgatta
     3421 ccgcacgcca ttgcgaagcg ccgcgctcat gcttgctcag gccgcgccgc tgatgctcgc
     3481 gctctttcta ctgttcccgc gcgcgaccgg accgctatgg cgcctgccgc aggacgcgca
     3541 cggtggggtg agcggccttt cggacacgat gacgcccggg agtgtcgccc gtctgacgtt
     3601 gtccgacgac gtcgccttcc gcgtcgaatt caagtcctcc attccccccg cccgcagcat
     3661 gtactggcgc ggcccggtgt tgtgggattt cgacggccgt acctggacca tgccgcgcag
     3721 ctttcctcaa gcaaacccca aattcgagtc actgtccccg gccgttgagt acacggttac
     3781 ggtcgagccg cacaaccggc tatggctctt cggtctcgat ctgccgtctg ctgcaccaca
     3841 gggcgccatc gtgagccgcg attttcagct ccgctcggtg gcgccggtcg tcaaccgcac
     3901 gcgctacgag atgcagtcgt accttgaata tcgtaccggg ctcgacgaaa gacgcaatgc
     3961 gctcgagcgc gccctgcagt tgccggaaag cgccaatccg cgcaccgtgc ggttcgcaaa
     4021 ggagctgaga aatcgccact cgacggatgc agcgctcatg cgcgaagtgc tgctcatgtt
     4081 ccgcaatgag aacttccatt acacgctcga gccgccgccc ctcgggccgc ata
//
LOCUS       DASURG010000491         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1003834, whole genome shotgun sequence.
ACCESSION   DASURG010000491 DASURG010000000
VERSION     DASURG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003834"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFY80_08655"
     CDS             complement(<1..1169)
                     /locus_tag="VFY80_08655"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6008019.1"
                     /translation="MFEVNIAAVLFVSMIVMVAMGVHIAVALGAASAIGVYLVTGNFA
                     VVERMLASTAYEALRDYVFAVIPLFMLMGEFIARSGAVTDVYRGINRGLRRLPGRLAL
                     ATVLGNALFSLVTGVSIASAAAFSRISYPEMKRHGYRRPFALGTIAGSSCLGMLIPPS
                     VLMIVWGILTEKSIGALFLAGVIPGVMVAGMFCLYILGVAIFRPDVVGAALPSGAAPQ
                     RPLASGASEEEESFNRGSFWGSTLGIALVVVTVLGGIWFGFFTPTEGAGAGAFIALVL
                     AIVKGMRWRGIVDCVLAVGRTSAPILILLVMASLYSRTLAMTGVTSAIQDAFLQTGLT
                     PAVILLIMVGIWFVLGMVIDSISIMLLTVIIFEPIAVSVGFDSIAFAVIGVLAIEA"
     gene            complement(1194..1733)
                     /locus_tag="VFY80_08660"
     CDS             complement(1194..1733)
                     /locus_tag="VFY80_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX6008020.1"
                     /translation="MPYLRYLIRFSDQLSKFLMILAAVWAMGLALLIVGDIIGNNMVG
                     EAIPGVREIVISSIVMIVYLQLGYAIRSHSMLRADTLLLVMPLLLRRIVLAIGYALGA
                     LLFFMILRGTISPAVHAWTTSEFWGEGALRVPVWPTRFMILVGCGLAILNYLVAALID
                     VFGLEDRPAARDIDIPGAD"
     gene            complement(1872..>2519)
                     /gene="dctP"
                     /locus_tag="VFY80_08665"
     CDS             complement(1872..>2519)
                     /gene="dctP"
                     /locus_tag="VFY80_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX6008021.1"
                     /translation="VFEDKFNQKLLARITDSSYNLGTNFEWKTLADLKGRRIAGAGLN
                     LNWLKYAGVTPVQSSLPEAYTSMQTRVYDGWIMFPSAWVNLKLHEVGPYYTLIGFGTI
                     TWHGLSINLNTWKRLPKDVQDIMLETAKDFEEQVGKVNRARYEKDIATLRGLIKVNEI
                     PANVRADWARALKDWPPSIAKELDGKGLPATQILKLTLDAAEKHGHKWPYRYEIK"
BASE COUNT          504 a          777 c          820 g          418 t
ORIGIN      
        1 gcttcgatgg cgagcacgcc gatcaccgcg aaagcgatcg agtcgaagcc gacggacacc
       61 gcgatcggct cgaagatgat gacggtgagc agcatgatcg agatcgagtc gatgaccatg
      121 cccagcacaa accagatccc gaccatgatg agcagtatca cggcgggcgt gaggccggtc
      181 tgaaggaagg cgtcctgaat ggcgctggtg accccggtca tggcgagggt gcgggaatag
      241 agcgatgcca tgacgagcag gatcagtatc ggcgcggacg ttcttcccac cgcgagcaca
      301 caatccacga taccgcgcca gcgcatgcct ttgacgatcg ccagcacgag tgcaatgaag
      361 gcgcctgcac cggcaccctc cgtcggtgtg aaaaacccga accagattcc gccgagcaca
      421 gtgaccacga ccagcgcgat gccgagcgtg ctgccccaga acgagcccct gttgaacgat
      481 tcctcttctt ctgaggcgcc ggaagcgagt ggcctttgcg gcgcagcgcc tgaagggaga
      541 gcggcgccga cgacatccgg ccgaaagatc gcaacgccga ggatgtagag gcagaacatt
      601 ccagcaacca tcacgccggg gatgacgccg gcgaggaaga gcgcaccgat cgacttctct
      661 gtcaggatgc cccacacgat catgagcacg ctaggtggaa tgagcatgcc gaggcaggac
      721 gagccggcga tggtcccgag tgcaaacggg cgccggtagc cgtggcgctt catctccgga
      781 taggagatgc gcgaaaacgc ggctgcggaa gcgatgctga cgcccgtcac gagcgagaac
      841 agcgcgtttc cgagtaccgt ggcgagggcg agccggccgg gaagacgccg gaggccgcgg
      901 ttgatgcccc ggtagacgtc ggtcactgca cccgagcggg cgatgaactc gcccatcagc
      961 atgaagagcg ggatcacagc gaaaacgtag tcgcgcagcg cctcgtatgc ggtgctcgcg
     1021 agcatgcgtt ccacgacggc gaaatttccg gtgacgaggt agacgccgat cgcgctcgcc
     1081 gcgccgagtg caacggcgat gtgcacgccc atcgccacca tgacgatcat gctgacgaac
     1141 agcacggcgg cgatgttgac ttcgaacacg gctactcctc ccccaaggct gctctagtcc
     1201 gcgccgggaa tgtcgatatc gcgcgcggcc ggccggtcct cgagcccgaa gacgtcgatc
     1261 agcgcagcga cgaggtagtt cagtatcgcg aggccgcagc ccacgaggat catgaagcgc
     1321 gtgggccaga ccggcacgcg cagcgcgcct tccccccaga attcggacgt ggtccaggca
     1381 tgcaccgcag ggctgatcgt ccctctcagg atcatgaaga acagcagggc gccgagcgca
     1441 tagccgatcg cgagcacgat acgccgcagg aggagcggca tgacgaggag aagcgtgtca
     1501 gcgcgcagca tcgaatggct gcgtatcgca tagccgagct gcaggtagac gatcatgacg
     1561 atggagctga tgacgatttc gcgcacaccg ggaatggcct cgccgaccat gttgttgccg
     1621 atgatgtcgc ccacgatcaa cagcgcgagc cccatcgccc agaccgcagc cagtatcatc
     1681 agaaacttgg aaagctggtc gctgaagcgg atgagatagc gcaggtaggg cattggggtg
     1741 ctcgggggat cgtgcgctaa gcacgcgacc gcggccggac acggttccga ccgcggctgc
     1801 gtgacaagtg accagcggca attacgcgca attcgcccgc actcgggtcg tcgtctgccg
     1861 ctcgcccggc gctatttgat ctcgtagcgg tagggccatt tatggccgtg cttctcggcc
     1921 gcgtcgagcg tgagcttgag gatctgcgtg gccggcaggc ccttgccgtc cagttccttc
     1981 gcgatcgaag gcggccaatc cttgagcgcg cgcgcccagt ccgcccgcac attcgcggga
     2041 atctcgttca ccttgatgag gccgcgcagc gtcgcgatgt ccttttcgta gcgcgcgcgg
     2101 ttgacctttc cgacctgctc ctcgaagtcc ttcgcggtct cgagcatgat gtcctgcacg
     2161 tccttgggca ggcgtttcca ggtgttcagg ttgatgctca acccgtgcca cgtgatggtg
     2221 ccgaatccga tgagcgtgta atacgggccc acctcgtgca gcttgagatt cacccacgcg
     2281 ctcggaaaca tgatccagcc gtcgtagacg cgggtctgca tgctggtgta ggcctcgggt
     2341 agcgagcttt gcaccggcgt cacgcccgcg tacttgagcc agttgagatt gagtccggca
     2401 ccggcgatgc gccgcccctt gagatctgca agcgttttcc actcgaagtt ggtgccgagg
     2461 ttgtagctcg agtcggtgat ccgcgcgagc agtttctggt tgaacttgtc ctcgaacac
//
LOCUS       DASURG010000492         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_595783, whole genome shotgun sequence.
ACCESSION   DASURG010000492 DASURG010000000
VERSION     DASURG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595783"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..745
                     /locus_tag="VFY80_08670"
     CDS             68..745
                     /locus_tag="VFY80_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008022.1"
                     /translation="MRRAVILCSALALWGSALSYAKLPPPTEEEIAKARAAKAKAAEQ
                     AKKDAAALAKAQDRVVEHYKRSKSGVAAAPAKDSTKKIAKKPRGKVERLACLTGSEDR
                     HARIGVEVVDSKVVSFAYYSKWKPRTCSVHVQRGDPYSKWQDYGAHTTVTTEKGTFLI
                     EHGRTEVQFMFREVDRMFYCGMEGKIRGSLTVKRGKRECVLEGVMDEHTEDAPEPAPS
                     TTAAAAS"
     gene            complement(771..1184)
                     /locus_tag="VFY80_08675"
     CDS             complement(771..1184)
                     /locus_tag="VFY80_08675"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX6008023.1"
                     /translation="MKWGAAPPWQIALIAVSLTSAGCEPVTLTAFGVGAAAGVQHTLT
                     GIAYRTFSVPLPKVRAAVLTAFDRMGIKLGGKESIENGERLTARASDREIEIELEAIT
                     PKTTRMRSTAKSGIFRDAATATEIILQTERVLGGS"
     gene            complement(1181..2404)
                     /locus_tag="VFY80_08680"
     CDS             complement(1181..2404)
                     /locus_tag="VFY80_08680"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6008024.1"
                     /translation="MALPLEGVKVVDLTTVMAGPYCCMVLGDMGAEVLKIENFPEGDS
                     ARRFDPQVNGESYCFAVLNRNKKSVALNMKDPRGKEAFMKLAAKADIITENFRPGVVR
                     KLGIDYQTISRINPGVIYASMSGFGQTGPYGRKGGFDIVAQGMSGIMMMTGYPGGRPA
                     KVGIAMNDIASGVTALYGILGAYISRLKSGKGQYLETSLLEAGLAWTHWESGAYFGSG
                     ELPTATGTRHRRSTPYQAYKTKDGYVTVGANNKKLWHNFCTMVCGKPEWLSDPRFIDL
                     PSRLKHIDQLEREIEAVFAIQPTAHWVEKLDAAEVPGGPVYTYDQILADPHIKARNMV
                     VEMDHPKIGRMKTLGLPVKSSGELVAIRRPAPWLGQHTEEALREAGYGRGEIEALFEA
                     GVIYDKHRASATASA"
     gene            2583..3551
                     /locus_tag="VFY80_08685"
     CDS             2583..3551
                     /locus_tag="VFY80_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008025.1"
                     /translation="MTLRFRFALPVVAAALFAPALAWGQAYPSKPVRVVIVFPPGGSN
                     DVVGRLVFQKVSEIAGQQFVVDNRGGAAGTIGSDIVAKSPPDGYTIMVQSTTHVANAH
                     LYKKLPFDVLKDFIGITPMARQVGMLVVHPSLPAKTGKEFVALARKRPGEIVYGSAGN
                     GSYVHLSMALMASMANIKMVHVPYKGGGPVNVAIASGEVQAVLATIGSLTANLKAKRV
                     RPLGVSSGERTKAYPDVPAIAEFVPDYEFTAWVGCFAPAGTPKPIIDALNGLIAKALA
                     DPGVAEKLSAQTLDPMHMTPEQFAQRLKSDYDKYEKVVKISDARID"
BASE COUNT          696 a         1121 c         1146 g          698 t
ORIGIN      
        1 ggcatcgcgg tttgcaccgg gcttactata ggtcgccgca tatgcggatg tgaaagggag
       61 gatgcgcatg agacgcgccg tgatattgtg ctccgcgctc gcactctggg gctcggccct
      121 cagttacgcc aaattgcccc cgccgacaga agaagaaatc gcgaaagctc gcgcagccaa
      181 ggccaaggca gccgaacaag ccaagaaaga tgccgcggcg ctggcgaagg cgcaggaccg
      241 cgtggtcgag cattacaagc gcagcaagag cggtgttgcc gccgccccgg cgaaggacag
      301 caccaagaag atcgccaaga aaccccgtgg caaggtcgag aggctcgcgt gcctcacggg
      361 cagcgaggac cgtcacgcgc gcatcggcgt cgaggtcgta gacagcaagg tcgtctcctt
      421 cgcctactac agcaaatgga agccgcgcac gtgctcggtg catgtccagc gcggcgatcc
      481 ctacagcaag tggcaggact acggcgccca taccaccgtg acgaccgaga agggcacctt
      541 tctcatcgag cacgggcgca ccgaggtcca gttcatgttt cgcgaagtgg accggatgtt
      601 ctactgcggc atggaaggca agatccgcgg cagcttgacg gtgaagcgcg gcaagcgcga
      661 gtgtgtgctg gagggtgtga tggacgagca caccgaggac gcacccgaac cggcgccctc
      721 cacgaccgcg gcagccgcca gctagtttcc cttaccgact ggctttgccg tcacgatccg
      781 ccgagcactc gctcggtctg aaggatgatt tcggtcgccg tcgcggcatc ccggaagatg
      841 cccgatttgg cggtggagcg catgcgcgtc gtcttgggcg tgatcgcctc gagctcgatc
      901 tcgatttccc gatcggaagc gcgtgccgtg agccgctccc cattctcgat cgattccttt
      961 cccccgagct tgatgcccat gcggtcgaat gccgtcaaca cggcagcgcg tactttcggc
     1021 aacggcaccg aaaaggtccg gtacgcgatg ccggtcagcg tgtgctgcac gccggctgcg
     1081 gcgccgacac cgaaagcggt caacgtcacc ggctcgcatc ctgcggaagt gagcgaaacg
     1141 gcgatgagcg cgatttgcca cggcggggcc gcgccccact tcatgcagag gccgtcgcgc
     1201 tcgcgcgatg cttgtcatag atgacgccgg cctcgaagag cgcctcgatt tcaccgcgcc
     1261 cgtagccggc ttcgcgcagc gcctcctcgg tatgctggcc gagccaaggg gcgggacggc
     1321 gtatggcgac gagctctccg ctggatttca ccggcaggcc gagcgttttc atgcgcccga
     1381 tcttgggatg atccatttcc accaccatgt tgcgtgcctt gatgtgcggg tcggcgagga
     1441 tctggtcgta ggtgtagacc ggtccgccag gcacttcggc cgcgtcgagc ttctccaccc
     1501 agtgcgctgt cggctgtatt gcaaataccg cttcgatctc gcgctcgagc tggtcgatat
     1561 gtttcaggcg cgacgggaga tcgatgaagc gcggatcgga cagccattcc ggcttgccgc
     1621 agaccatcgt gcagaagttg tgccacagct tcttgttgtt cgccccgacc gtgacgtagc
     1681 catctttcgt cttgtaggcc tggtacggcg tcgagcggcg gtggcgcgtg ccggtcgcgg
     1741 tcggcaactc gcctgagccg aagtaggcgc ccgactccca gtgggtccac gcgagccccg
     1801 cttcgaggag cgaggtctcg agatactgac ccttgccgct cttgagacgg ctgatgtagg
     1861 cgccgaggat gccgtagagc gccgtgacgc cgcttgcgat gtcgttcatc gcgatgccca
     1921 ccttggcggg acggccgcct ggatacccgg tcatcatcat gatcccagac atgccctgcg
     1981 cgacgatgtc gaagccgcct ttgcggccgt acggcccggt ctggccgaaa ccggacatgg
     2041 aggcgtagat gacgccggga ttgatcctgc tgattgtctg gtagtcgatg ccgagcttgc
     2101 gcaccacacc cggcctgaaa ttctcggtga tgatgtcggc ctttgcggcg agcttcatga
     2161 acgcttcttt gccgcgcgga tctttcatgt tgagcgcgac gcttttcttg ttgcgattga
     2221 gcaccgcaaa acagtacgac tcgccgttta cttggggatc gaagcgtcga gcggagtcgc
     2281 cttcgggaaa attctcgatc ttgagcactt cggcgcccat gtcaccgagc accatgcagc
     2341 agtagggacc ggccatgacg gtggttaagt cgaccacttt cacgccttcg agtggaagtg
     2401 ccatcgcgtg tctgctctcc ggagaccccg cgccccgatc gggacgcgga agccgttaaa
     2461 aatgaagaga attatacaat cgctgccctg gggttttcag ccgccgattg aacgggacgg
     2521 catattctgc tcataatgcc gaacaaaatc acgcggttcc catagtcgag gaggagtatg
     2581 tcatgacgtt gcgtttccgt tttgcactgc ccgttgttgc agccgccctg ttcgcccccg
     2641 cgcttgcgtg gggccaggcc tatccatcca agcctgtccg agtggtcatc gtgtttcctc
     2701 caggcggttc gaacgacgtg gtgggccggc tggttttcca gaaagtgtcg gaaatcgccg
     2761 gacagcagtt tgtcgttgac aatcgcggcg gcgcggccgg aacgatcggc tcggacatcg
     2821 tggccaagag tccgcccgac ggctacacca tcatggtgca gtccaccacg cacgtcgcca
     2881 acgctcatct ctacaagaag cttccgttcg acgtactgaa agactttatc ggtatcaccc
     2941 ccatggcaag acaggtcggc atgcttgtgg tccatccctc gctcccagcg aaaacgggca
     3001 aggaattcgt cgcgctggca cgcaagcgcc ccggcgaaat cgtctatggg tctgccggca
     3061 atgggagcta cgtgcatctc tccatggcgc tgatggcgtc tatggccaac atcaagatgg
     3121 tgcacgtccc ctacaagggt ggcggccccg tcaacgtcgc catcgcttcc ggcgaagtcc
     3181 aggctgtgct cgcgacgatc ggctcgctca ccgcgaatct caaggcgaaa cgcgtgcggc
     3241 cactgggggt gagttcgggc gaacgcacca aggcctatcc cgatgtcccg gcaatcgccg
     3301 agttcgtgcc cgactatgaa ttcacggcgt gggtcggttg cttcgccccg gcggggacgc
     3361 cgaagccgat tatcgacgcg ctgaacggat tgatcgcgaa ggcgttggcg gatccgggcg
     3421 tggcggaaaa actctcggcg cagacgctcg accccatgca catgacgcca gagcagttcg
     3481 cgcagcgact taaatccgac tatgacaagt acgaaaaggt cgtgaaaatc agcgacgcgc
     3541 ggatcgattg aaggtaaagc gagcgcgtcg aggcacggcg gtttgtcgcc aaatcttgac
     3601 gctacgtcgt agaacctcta cataagttac tgaaattaaa ggatgtagag gtttacatcc
     3661 t
//
LOCUS       DASURG010000493         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1310603, whole genome shotgun sequence.
ACCESSION   DASURG010000493 DASURG010000000
VERSION     DASURG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310603"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFY80_08690"
     CDS             <1..597
                     /locus_tag="VFY80_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucC family protein"
                     /protein_id="HEX6008026.1"
                     /translation="NKAILRVALPAYFASLIALAFAALPEPHALTLPLLYVIHVVMGI
                     ASGGIGLATGNIGLKLAPQGKGTAYLTAVGLAGSFAAGMAALAGGQLADWFAARQLSL
                     TFQWTAPDAMKQMTLLQFRHWEFLFGISFALGFYVLHRLSRIREGAEHSERTVVQQFV
                     IEAVRSLDQLSPIEGLRTVILLPFARLRDRRVKPRPPP"
     gene            696..896
                     /locus_tag="VFY80_08695"
     CDS             696..896
                     /locus_tag="VFY80_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008027.1"
                     /translation="MTRDFPSIELLEARCSDFAKALLREVADHPKYHQIASKLMALDG
                     TIFRSDIERALYEVSIEDPKRA"
     gene            complement(930..>2313)
                     /gene="aprA"
                     /locus_tag="VFY80_08700"
     CDS             complement(930..>2313)
                     /gene="aprA"
                     /locus_tag="VFY80_08700"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HEX6008028.1"
                     /translation="EVYNRIMVTHLLMDAKRPNRVAGAVGFSVRDGTFYVFRAKAVII
                     GAGGGSHIYRPRSVGEGMGRTWYAPWSTASAYGLIIPTGAKMIQMENRIVLARFKDGY
                     GPVGAWFLLLKSHATNAYGEMYEAARFDEIKARVGSYAEITPMPTCLRNHAMLEEMKA
                     GKGPIYMHTQKVLDTKEKEEIGWEDFLDMTISQAVVWAAQNIDPKEKPSELITSEPYV
                     MGSHATCSGAWASGPEDCAPSDYQWGYNRMTTVEGLFGAGDTIGGSAHKFSAGSFTEG
                     RLAGKAAVRYVLDRKSESPQVSDEHVAELRAQIYQPLETYRVGRNEIVAGTVAPGYIT
                     PLAGLQRLEKLMDEYVGGYSTFYVTNEPLMNRGLELLEMLKEDMAHVGAEDLHQLQRA
                     WELHHRIMTAESVTRHTLFRKETRWPGYYYRADFPKLVDPDWHVFTASQYDPKTRHWE
                     MTKLPVHHFV"
BASE COUNT          378 a          756 c          718 g          461 t
ORIGIN      
        1 aacaaggcga tcctgcgcgt cgcgctgccc gcctacttcg caagcctcat cgcacttgcc
       61 ttcgccgcgc ttccggagcc gcatgcgctc acgctgccgc tcctctacgt aatccacgtc
      121 gtgatgggca ttgcgtcggg cggtattggg cttgcgaccg gcaacatcgg tctcaagctt
      181 gcgccgcagg ggaaaggaac cgcctatttg acggcggtcg ggctcgccgg atcgttcgcg
      241 gccggcatgg cggcgttagc cggcggccaa ctcgcggact ggttcgccgc gcgccagctc
      301 tcgctcacgt tccagtggac cgctcccgat gcgatgaagc aaatgacgct ccttcaattc
      361 cggcactggg aattcctgtt cggaatctcg tttgcactcg gcttctacgt gttgcaccgg
      421 ctctcgcgca tacgtgaggg ggccgagcac agcgagcgca cagtggtgca gcagttcgtg
      481 atcgaggcgg tgcgctcgct cgaccagctt tcgccgatcg agggtctgcg caccgtgatc
      541 ctgttgccct ttgcgcggtt gcgcgaccgc cgtgtcaagc cgcgcccgcc gccttgacgc
      601 tcgatgcgcc accgagctac gtgtcggcgt gtctccgaca gcctttcgcg cgctggacgc
      661 ggtaaactgc gcgggaaacc ggacgaagcg atcccatgac ccgcgatttc ccttcgatag
      721 agcttcttga agcccgctgc agcgacttcg ccaaggcact gctgcgcgaa gtcgccgacc
      781 atcccaagta ccatcagatt gcgagcaagc tcatggcgct cgatggaacg atcttccgtt
      841 ccgatatcga gcgggcgctc tacgaagtga gcatcgaaga ccccaagcgg gcatgaaggc
      901 ggcggtgagc cgcctttgtc acgtacggtt cagacgaagt ggtgtaccgg gagcttggtc
      961 atctcccagt ggcgcgtctt cgggtcgtat tgcgaggcgg tgaagacgtg ccagtcggga
     1021 tcgacgagct tcgggaagtc agcgcgatag taatagcccg gccagcgcgt ctccttcctg
     1081 aagagcgtgt ggcgcgtcac cgattccgca gtcatgatcc ggtgatgcaa ctcccacgcc
     1141 cgctggagct ggtgcaagtc ctcggcgccg acatgcgcca tgtcttcctt cagcatttcg
     1201 agcagctcga gcccgcgatt catgagaggc tcattggtga catagaacgt ggagtagccg
     1261 ccgacatatt cgtccatcag cttctcgagg cgctgcaatc cggcgagcgg cgtgatgtag
     1321 cccggtgcca cggttccggc gacgatttcg tttctgccga cgcgataggt ctcgagcggc
     1381 tggtagatct gcgcccgcag ctccgcgacg tgctcgtcgc tcacctgcgg cgattcgctc
     1441 ttgcgatcga gcacgtagcg caccgccgct ttgccggcaa ggcggccttc ggtgaacgaa
     1501 cccgcggaga atttgtgcgc cgaaccgccg atcgtgtcgc cagcgccgaa gaggccttcc
     1561 accgtcgtca tgcggttgta gccccactga tagtctgaag gcgcgcagtc ttccggcccg
     1621 ctcgcccacg cgcccgaaca cgtcgcgtgc gagcccatga catagggttc ggacgtgatg
     1681 agctccgagg gtttttcctt ggggtcgatg ttctgcgccg cccatacgac ggcctggctg
     1741 atcgtcatgt cgaggaagtc ctcccagccg atctcttcct tttccttggt gtcgaggacc
     1801 ttctgcgtgt gcatgtagat cgggcccttg ccggccttca tctcttccag catcgcgtga
     1861 tttcggagac acgtcggcat cggcgtgatt tcggcatagc tgcccacacg cgccttgatc
     1921 tcgtcgaagc gcgccgcttc gtacatctcg ccgtaggcgt tggtcgcgtg cgacttcagc
     1981 agcaggaacc acgcgccgac cggaccgtag ccgtccttga agcgggcgag cacgatgcga
     2041 ttctccatct ggatcatctt cgcgcccgtg ggaatgatga gcccataggc ggacgccgtg
     2101 ctccacggtg cataccacgt gcgccccatg ccttcgccca ccgagcgcgg gcgatagatg
     2161 tgcgagccac cgccggcgcc gatgatcacc gccttggcgc ggaaaacgta gaaggtgccg
     2221 tcgcgcacgc tgaagcccac cgcgcccgca acgcggttcg gccgcttcgc gtccatcagg
     2281 aggtgcgtaa ccatgatgcg gttgtagacc tcg
//
LOCUS       DASURG010000494         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1516077, whole genome shotgun sequence.
ACCESSION   DASURG010000494 DASURG010000000
VERSION     DASURG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516077"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..313)
                     /locus_tag="VFY80_08705"
     CDS             complement(5..313)
                     /locus_tag="VFY80_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008029.1"
                     /translation="MAQCIEGRFPPEIQAGVLERALMRATEWSNREYGKECVVCADFL
                     WQDDETFTFHITSPDLPDMLINTSAEITVTKAHATLVDSRKFHSCYVKRAKSGVSGGG
                     "
     gene            570..2105
                     /gene="istA"
                     /locus_tag="VFY80_08710"
     CDS             570..2105
                     /gene="istA"
                     /locus_tag="VFY80_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX6008030.1"
                     /translation="MFQYRQVLIRLRQGDGDREIARSGLMGRVKVARLRGLATERGWL
                     AEGARLPDDAELAAAVGRARRARSTISSAEPHRKVIERWREQGVSGVAIHAALCREHG
                     YTGSYSAIHRIIASIEQAQPPKATVPLTFAPGEAAQVDFGAGPLLVDPADGRVKRTWC
                     FVMTLCFSRHQYAEFVFDQKVATWLGLHRRAFEWFGAVPGRLIIDNPRCAITRACSRD
                     PIVQRAYAECAEGYGFRIDPCPPADPAKKGIVEAGVKYVKRNFLPVRQFRDLADLNAQ
                     VREWVLTEAGERIHGTTRERPLERFALEKPLLRPLPDIAPDLGVWACVRVHRDCHVQF
                     ERALYSVPFKLVGRDLWLRATDTTVTVYEEHQLVAVHLRTRSAGTRRTVSEHLPPEAR
                     EFFARDRHWCLTQAERIGPSCVELIRTLFADRILERLRAAQGVLRLAEQFGAARLEAA
                     CARSLAHASPHYRTVKTILARGFDRVDVAQQFREHSAGVYAAEARFVRPADLLFATGE
                     PQS"
     gene            2251..>2835
                     /locus_tag="VFY80_08715"
     CDS             2251..>2835
                     /locus_tag="VFY80_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6008031.1"
                     /translation="MNPVPELSPHLKQLRLSGILDSLEARNRQAIESKLAYTEFLSLL
                     IQDEVARREQKKFALRLRRATFRTTKTLEQFDFERLPKLNRALVHELATGRYLTERAP
                     VLIVGPCGTGKSHLAQALGHCAIRQGVDVLFTTCAQLTASLNAARATGAYDRKLAALA
                     RIPLLCIDDFGLKPLRPPADEDLHDLIAERYEQTS"
BASE COUNT          474 a          977 c          871 g          513 t
ORIGIN      
        1 cggctcaacc accgccgcta actccgcttt ttgcacgctt cacgtagcag gagtggaatt
       61 tcctagagtc gacgagcgtg gcgtgcgctt tcgttacagt gatttccgcg gacgtgttta
      121 tcaacatgtc gggaagatcg gggctcgtga tgtggaaggt gaacgtttca tcgtcctgcc
      181 acagaaagtc agcgcagaca acgcactctt taccgtattc gcgattcgac cactccgtcg
      241 cccgcatcag cgccctttcc agcacgccgg cctggatctc gggcgggaat cggccctcaa
      301 tgcactgcgc catcggtggt gccgacacac tcgcacaacc cacaagctct gccaatgacg
      361 ctgcgaggac gattaggctg acgattctcg cgcttgtcat ctttcggcgt aatggagcca
      421 atcggttttc ggcataaagg agccagtcag ttttcggcat attggagcca gtgaaggggt
      481 gagcacgggc atctcggatt gacggcggcc gccgtaccct cgggcctttt tccggccgag
      541 gaagcacggt gggtcgcagg agattcgaga tgtttcagta tcgacaggtc ctcatcaggc
      601 tgcgccaagg ggacggcgat cgggagatcg cccgctcggg actcatgggg cgggtgaagg
      661 tagcgcggct gcggggcctc gccacagaac gaggctggct cgccgaaggc gcccggttgc
      721 ccgacgacgc cgagctcgct gcggcggtcg gccgagcgcg gcgtgcccgc tcgacgatct
      781 cgagcgcgga accgcaccgc aaggtcatcg aacgctggcg cgagcaaggc gtttccggcg
      841 tcgcgatcca cgcggcgcta tgccgtgagc acggctacac cggcagttac tcggcgatcc
      901 atcggatcat cgcctcgatc gagcaagcgc aaccgccgaa ggcgacggtg ccgctgacgt
      961 tcgcgccggg cgaagcggca caggtcgatt tcggtgccgg cccgctgctc gtcgatcccg
     1021 ccgacgggcg cgtgaagcgc acctggtgct tcgtgatgac gctgtgcttc tcgcgccatc
     1081 agtacgcgga gttcgtattc gaccagaagg tggcgacttg gctcgggctg catcggcgcg
     1141 catttgagtg gttcggtgcc gtgcccggcc ggctgatcat cgacaacccg cgctgcgcga
     1201 tcacccgtgc ctgcagtcgc gacccgatcg tgcagcgcgc ctatgccgag tgtgcggaag
     1261 gctacggctt tcgcatcgat ccatgcccgc cggccgatcc ggccaagaag ggcatcgtcg
     1321 aagccggcgt gaagtacgtg aagcgcaact tcctccccgt ccgccagttc cgcgatctcg
     1381 ccgatctcaa cgcccaggtc cgcgagtggg tcctcaccga agccggcgaa cgcatccacg
     1441 gcaccacgcg cgagcggccg ctcgagcgtt tcgcactcga gaagccgctg ctgcgcccgc
     1501 tccccgacat tgcacccgac ttgggcgtat gggcgtgcgt gcgcgtgcat cgagattgcc
     1561 atgtgcagtt cgagcgggcg ttgtactcgg tgcccttcaa gctcgtcggc cgagatcttt
     1621 ggctgcgcgc caccgacacg accgtgaccg tttacgagga gcaccaactc gtcgccgtgc
     1681 atctgcgcac tcggtcagcc ggtacgcgcc gaaccgtgag cgagcatctg ccgccggagg
     1741 cgcgggagtt cttcgctcgc gatcggcact ggtgtcttac acaggccgag cggatcggcc
     1801 cgagctgcgt cgagctcatc cgcacactct tcgccgatcg gatcctcgag cgcttgcgtg
     1861 ccgcgcaagg cgtgctgcgg cttgccgagc agttcggcgc cgcacggctc gaagccgctt
     1921 gtgctcggtc gctcgcacac gcctcgcctc actaccgcac cgtcaagacg atcctcgccc
     1981 gcggtttcga ccgtgtcgac gtcgcccagc agtttcgcga gcacagcgcc ggcgtctacg
     2041 ccgcagaggc gcgcttcgtg cgaccggccg acctgttgtt cgcgaccggc gagccgcagt
     2101 cgtgattctc gagggtaccc aaggccaggc ccgcctgccg atcgcggcgc ctgggcgatt
     2161 ctacgcggcc gatttttctc gcaacgagct cgaacgccca ggttgcggcg ttctctcgtc
     2221 attcacccca agcaaccgaa ggagtcctct atgaaccccg ttcctgagct cagtcctcat
     2281 ctgaagcagc tacggctctc cggcatcctc gactcgctcg aagcgcgtaa ccgccaagcc
     2341 atcgagtcga agcttgccta taccgagttc ctgtcgctat tgatccaaga cgaagttgcc
     2401 cgacgagagc agaagaagtt cgcgctgcgc cttcgccgcg ccacgttccg caccaccaag
     2461 acactcgagc agttcgattt cgagcgcttg cccaagctca accgcgcgct cgtccacgag
     2521 ctcgccaccg gccgctatct caccgagcgc gcccccgtgc tcatcgtcgg tccctgcggc
     2581 accgggaagt cgcatcttgc acaggcgctc ggccactgcg cgattcgtca gggcgtcgac
     2641 gtgctgttca ccacctgcgc gcagctcacc gccagcctca atgccgctcg cgccaccggc
     2701 gcctacgacc gcaagctcgc cgcactcgct cgcattccgc tcctgtgcat cgatgacttt
     2761 ggcctcaagc cattacggcc gccggccgac gaagaccttc acgacttgat cgccgagcgc
     2821 tacgaacaga cgagc
//
LOCUS       DASURG010000495         6584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_391559, whole genome shotgun sequence.
ACCESSION   DASURG010000495 DASURG010000000
VERSION     DASURG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391559"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFY80_08720"
     CDS             <1..627
                     /locus_tag="VFY80_08720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX6008032.1"
                     /translation="SQILGYLVAPRRGELVVDFCAGAGGKALMLGALMQSHGRVYAFD
                     VSARRLARLGPRLKRSGLSNLHPAVIANENDTRVKRLAGRIDRVLVDAPCSGLGTLRR
                     NPDLKWRQSPRSVAELATKQAAILEGAARLVKPGGRLVYATCSLLAEENEAIVKAFLT
                     AHATFALLDCGQLLKAQRIALDTGQYLSLLPHVHGTDGFFAAAISRTG"
     gene            complement(642..2864)
                     /locus_tag="VFY80_08725"
     CDS             complement(642..2864)
                     /locus_tag="VFY80_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6008033.1"
                     /translation="MPLPLKILLLEDYAADAELALRELERDGVDCTMRRVETEEAFVR
                     MLDEFHPDVILSDFTLPAFDGLTALEIARKVEPDIPFIFVSGTLGEERAIESLKRGAV
                     DYVLKTNLRRLAPAVRRALQECVERKERRKAEQRVARLSRIHAVLSGINSTIVRVRQR
                     EELFRDACRIAVVHGGFRFAWIGLVDPETLDIRPAAWMGQDDGYLAGLKLSASGAVSE
                     EQDAAGLAVRSRSAVVINDVATDSRLRDAGRALDRGFRALVVLPLVIDEKAAGVLGLY
                     ADEPDIFDEEELKLLGELAANIAFALDHIAKTERADYLAYYDGLTGLANRTLFSDRVA
                     QYLHAAARGESVAILMIDLERFRHINETFGVAAGDAVLRTFAKRLAQAVPEHGTVARI
                     NADRFGVAVAGIRSVSDVARLLEQELTTALAQPFEASSQQVHIPGRAGVAVSPGDGSD
                     AATLCTNAETALQDAKRRSEIFMFYAPEMNARVAERLTLENRLRVALKEGEFTLHYQP
                     KIDLATGAISGLEALLRWNSPDLGMVLPERFISILEDTGMIVEVGLWAVRRAIADQLA
                     WRKRGLAPPRTAVNVSTVQLRHARFVNEVSDLVEAGGAAGRHIDLEITESMIMADIAS
                     SMEKLSAIRALGVGLSLDDFGTGYSSLSYIARLPVITIKIDRSFIAAMRESAEQLAIV
                     SAVISLARALRLRVVAEGVETSEQARLLKELGCDEAQGFHYSRALPAQEVAALFARKT
                     "
     gene            3046..4209
                     /locus_tag="VFY80_08730"
     CDS             3046..4209
                     /locus_tag="VFY80_08730"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX6008034.1"
                     /translation="MFTGALELPWWGYVVHTLIVTHITIAAVTIFLHRHQAHRALDLH
                     PLPSHFFRFWLWLTTGMVTKEWTAIHRKHHAKCETDEDPHSPQIVGIKKVLWEGTELY
                     REESRNKETLERYGHGTPDDWIERHVYTLHSVMGIALLFIVNFALFGFIGITIWAVQM
                     VWIPFFAAGVINGIGHFWGYRNFQPEDASRNIVPWGILIGGEELHNNHHAYASSARLS
                     NRWFEFDIGWLYIRMLESLQLAQVKKVAPKVRLVADKKHCDLATVQAVVTHRYEVIAK
                     YAKSLRRTCAHELRTLHARAVTVDRATLRRWLHIDAGTLPTIERAKMNEVLSKSSVLN
                     TIYTMRQELAVLWQRSNASKEQLVGHLEDWCRRAEASGIAALQEFSRQLRCYA"
     gene            complement(4334..4489)
                     /gene="rpmG"
                     /locus_tag="VFY80_08735"
     CDS             complement(4334..4489)
                     /gene="rpmG"
                     /locus_tag="VFY80_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX6008035.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRTTPDKIEIKKFDPVARKHVL
                     YKETKIK"
     gene            complement(4501..4737)
                     /gene="rpmB"
                     /locus_tag="VFY80_08740"
     CDS             complement(4501..4737)
                     /gene="rpmB"
                     /locus_tag="VFY80_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX6008036.1"
                     /translation="MARVCQVTGKKPMLGNNVSHANNKTRRRFLPNLQYRRFWVEKEN
                     RWVSLRVSQAGLRTIDKKGIEAVLAEIRARGESI"
     gene            complement(4810..5484)
                     /gene="radC"
                     /locus_tag="VFY80_08745"
     CDS             complement(4810..5484)
                     /gene="radC"
                     /locus_tag="VFY80_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX6008037.1"
                     /translation="MAITDWPLNERPREKLLGKGAESLSDAELVAIFLRTGLKGKSAV
                     DRAREVLSAFGSLSALCAADSARFCGIPGLGSAKYVQLQAVLEMARRSLREQLARSTV
                     LGSPQAVRDYLRLKLQDRPHEVFVGVFLDAQNRVLAVEELFRGTLTQTSVYPREVVKR
                     ALHHNAGALIFAHNHPSGVAEPSRADEALTHALKQALALVDVRVLDHFVIGAGTAMSF
                     AERGLI"
     gene            5612..>6584
                     /gene="coaBC"
                     /locus_tag="VFY80_08750"
     CDS             5612..>6584
                     /gene="coaBC"
                     /locus_tag="VFY80_08750"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX6008038.1"
                     /translation="MTDPARKKVLLGLTGGIAAYKAAELCRLLVKDGIDVQVVMTEAA
                     RGFITPATMQALSGKPVFTDMWDERVPDNMGHIELSRGKDAIVVAPATADFIAKLVHG
                     LGDDLLSTLCLARECPLVVAPAMNRQMWDNAATRRNMARLAADGVAIVGPASGDQACG
                     EVGMGRMVEPDAIAEAVRALWGPKALRGVRVMVTAGPTFEAIDPVRGITNRSSGKMGY
                     AIARAAIDAGARVTLISGPTALATPAGTERVDVVSAEEMCGAVKRRISEVDIFVGVAA
                     VGDYRPVRQSEHKIKKSTRNLTIELEPNPDILAYVAELPNRPFCVGFA"
BASE COUNT         1211 a         2100 c         2073 g         1200 t
ORIGIN      
        1 agtcagattc tgggctatct cgtcgcgccg cgtcggggcg agctggtcgt cgatttctgt
       61 gccggcgccg gaggaaaagc gctcatgctg ggagcgctca tgcaatcgca cgggcgcgtc
      121 tatgctttcg atgtgtcggc ccgacggctc gcgcgactcg ggccgcggct caagcgctcg
      181 ggactcagca atctccatcc ggccgtcatc gcgaatgaaa acgatacccg ggtcaagcgc
      241 ctcgccggca gaatcgatcg cgtgctggtg gatgccccgt gcagcggcct ggggacgctg
      301 cgccgcaacc ccgatctcaa gtggcgccag tcgcctcgct cggtcgcgga acttgcaaca
      361 aaacaggccg cgatcctgga aggggcggcg cgactcgtca agccgggcgg gcggctcgtc
      421 tatgcgacct gcagcctgct cgcagaggag aacgaggcga ttgtgaaagc atttctcact
      481 gcacacgcca cattcgcact gcttgattgc gggcagctgc tcaaggcgca gcgcattgcg
      541 ctcgacaccg ggcagtacct gtcactgctc ccgcatgtgc acggaaccga tggctttttt
      601 gccgccgcca tatcccgcac gggctgaggc tcttggccgc atcacgtttt ccgcgcgaag
      661 agagccgcca cctcttgggc cggcagcgca cggctgtagt ggaacccttg cgcctcgtcg
      721 cacccgagct ctttcagcag ccgggcctgc tcgctggttt cgacgccctc ggcaacgacc
      781 cgcaggcgca gtgcgcgtgc gagtgaaatg accgccgaga cgattgcgag ctgctcggcg
      841 ctctcgcgca ttgccgcgat gaaggagcgg tcgatcttga tcgtgatcac cggaagtcgc
      901 gcgatgtaac tgagcgacga atagccggtt ccgaagtcgt ccagggacag gcccacgccg
      961 agcgcccgga tggcggagag cttttccatg ctgctcgcga tatccgccat gatcatgctc
     1021 tccgtgatct cgaggtcgat gtgccggccg gccgccccgc cggcttccac gagatcgctg
     1081 acttcgttta cgaaccgcgc atgccgcaac tggacggtcg agacgttgac cgccgttcgc
     1141 ggcggggcaa ggccgcgctt gcgccatgcg agctggtcgg caatcgcccg gcgcacggcc
     1201 cacaggccga cctcgacgat catgccggtg tcctccagga tggagatgaa ccgctcgggg
     1261 agcaccattc cgagatccgg gctgttccag cgcaacaacg cctcgagtcc gctgatggcg
     1321 ccggtggcga gatcgatctt gggctgatag tgcaaagtga actcgccctc tttcagagcg
     1381 acgcgcagcc ggttttcaag cgtgagccgc tccgccaccc gcgcgttcat ttcgggcgca
     1441 tagaacatga agatttcgct gcggcgcttc gcatcctgca gcgcggtctc ggcgttggtg
     1501 cagagtgtgg cggcatcgga tccatcgccg ggtgaaacgg caaccccggc acgcccgggg
     1561 atgtgcacct gctgtgagct ggcctcaaac ggttgcgcaa gggctgtggt cagctcttgc
     1621 tccaacagac gtgcgacatc ggaaacgctc ctgatcccgg caacggcgac gccgaaacgg
     1681 tccgcattga tgcgcgcgac ggtgccatgc tcaggaaccg cctgtgccag gcgcttcgcg
     1741 aacgttcgta gaaccgcgtc gccagcggcc accccgaacg tttcattgat gtgccggaac
     1801 cgctcgagat cgatcatcaa tatcgcaacg ctctcgccgc gtgcggcggc atgcagatac
     1861 tgcgccacgc ggtcgctgaa gagcgttcgg ttggcaagac cggtcaggcc atcgtaatag
     1921 gcgagatagt cggctcgctc cgtctttgca atgtggtcga gcgcgaaggc gatgttggcc
     1981 gcgagttcgc ccagcagctt gagttcttcc tcatcgaaga tatcgggctc gtcggcgtag
     2041 agaccgagca cgcccgccgc cttctcgtcg atcacgagcg gcagcacgac gagcgcgcgg
     2101 aatccgcggt cgagcgcccg cccggcatcg cgcagtctcg aatcggtcgc gacgtcattg
     2161 atgacaacgg cggaccggct gcgcacggcg aggcccgccg catcttgttc ttccgataca
     2221 gcgccggatg cggagagctt gagcccggca agatagccgt catcctgccc catccaagcg
     2281 gcggggcgga tgtcgagcgt ctcgggatcg accaatccga tccaggcaaa cctgaaaccg
     2341 ccatgcacca cggcgatgcg gcatgcgtcc cgaaagagct cttcgcgctg gcgtactctc
     2401 acgatcgtcg aattgattcc ggacagcacg gcgtggatgc ggctcagccg cgcgacgcgc
     2461 tgctcggcct tgcggcgctc cttgcgctcg acgcactcct gcagcgcgcg ccgcaccgcc
     2521 ggcgcaagcc ggcgcaagtt ggtcttcagc acgtaatcga ccgcgccgcg cttgagcgat
     2581 tcgatggcac gctcctctcc gagagtgcct gagacgaaaa tgaaagggat gtcgggctct
     2641 actttgcgcg cgatctcaag cgccgtgaga ccgtcgaaag cgggtaaggt gaaatcggac
     2701 aagatcacgt cggggtggaa ctcgtccagc atgcgcacga aggcctcctc cgtctcgacc
     2761 cggcgcatgg tgcagtcgac gccatcgcgc tccagttccc tcaacgccag ctccgcgtcg
     2821 gcggcataat cttcgagcag aaggattttc aggggcaatg gcacgtgggt ttgggctcgc
     2881 ggcaagaccg taaatatgac ccaatcacta ttccatacaa gctcttaagc gcgccatgtg
     2941 ccgtctggcg tgcaacggca tgcaacatca aatttctttg cgaaatcaga gaacttctgt
     3001 aaactcgccg cttctaaaga gactttgaag ccaagaaggg ggttcatgtt tacaggggcg
     3061 ttggaactgc cgtggtgggg ctatgtcgtc catactctca tcgtcaccca catcacgatc
     3121 gcggcagtca cgatctttct tcaccggcat caggctcacc gcgcgctcga tctccatccg
     3181 ctgccgagtc attttttccg cttctggctg tggctcacga caggaatggt gaccaaggaa
     3241 tggaccgcca ttcaccgcaa gcaccacgcg aagtgcgaga ccgatgagga tccgcacagc
     3301 ccgcagatcg tcggcatcaa aaaggtgctt tgggaaggca cggagctcta ccgcgaggaa
     3361 tcccgcaaca aggaaacgct ggagcggtac ggccacggca cgcccgacga ctggatcgaa
     3421 cgccacgttt acacccttca ctcggtgatg ggcattgcgc tgttgttcat tgtcaatttc
     3481 gcgctgttcg gcttcatcgg catcacgatc tgggcagtgc agatggtgtg gattcccttc
     3541 ttcgccgccg gggtgatcaa cggcatcggg cacttctggg gttaccgcaa ctttcagccg
     3601 gaagacgcga gccgcaacat cgttccgtgg ggcatcctca tcggcgggga agagctgcac
     3661 aacaaccatc atgcctacgc gagctccgcg cggctgtcga accgctggtt cgaattcgac
     3721 atcggatggc tctacatccg catgctggag agcctccagc tcgcgcaggt gaagaaggtc
     3781 gcgcccaaag tgaggctcgt cgccgacaag aaacactgcg atctcgcaac ggtgcaggcc
     3841 gtggtcacgc accgctacga agtgatcgcc aagtatgcga agtctctgcg gcgcacctgt
     3901 gcgcacgagc tgcggacgtt gcatgcgcgc gctgtgacgg tagaccgggc aacgctgcga
     3961 cgctggctgc acatcgacgc gggcacctta ccgacgatcg agcgcgcgaa gatgaacgaa
     4021 gtgctgagca agagcagcgt gctcaatacc atctacacca tgcgccagga gctcgcagtg
     4081 ctgtggcagc gctccaacgc atccaaggag caactggtcg ggcacctcga agactggtgc
     4141 cgccgcgccg aggcgagcgg gatcgcggcc ctgcaggaat tcagcaggca gttgcgctgt
     4201 tacgcataga gagccgcgaa cgaacgactc ggagaaaaaa cagccacgaa aaagcccgcg
     4261 caaagcgggc ttttttctgc actgcgcgtt caaacggcac ctcgagtcgg ttcaaacgcg
     4321 acagcgtatg ccgtcacttg atcttggttt ccttgtagag gacgtgcttg cgcgcgaccg
     4381 gatcgaactt cttgatttcg atcttatccg gcgtggttcg cttgttcttg gtggtggtgt
     4441 agaagtgacc ggttccggcg ctcgactcga gcttgatctt ctcacgcata tgagttctcc
     4501 ttaaatgctt tcgccgcggg cccgtatttc ggccagcacg gcttcgatgc ccttcttgtc
     4561 tatcgtccgc aaaccggcct gggagacccg aaggctcacc cagcggtttt ccttttcgac
     4621 ccagaagcgg cggtactgca ggttggggag aaagcgccgc cgcgtcttgt tgttggcatg
     4681 ggaaacgttg tttcccagca tcggcttctt gccggtgacc tgacacacgc gcgccatggc
     4741 aatggctcca gtattttcga aggggcggat tctacacgct tttcggcgtc cgcctcaagt
     4801 cgccgcggct cagatgagcc cgcgttcggc gaacgacatc gccgttcccg caccgatcac
     4861 gaaatggtcg agcaccctca cgtccaccag ggcgagcgcc tgcttcagcg cgtgcgtgag
     4921 cgcttcgtcc gcccggcttg gctcggcgac acccgaagga tgattgtgag cgaagatgag
     4981 ggcgcccgca ttgtggtgca gcgcgcgctt cacgacttcg cgggggtaga cgctcgtctg
     5041 cgtcagcgtg ccgcggaaga gttcctccac ggcgagcacg cggttctgcg catcgagaaa
     5101 tacgccgacg aacacctcgt gcggccggtc ctgcagcttg agccgcagat aatcgcggac
     5161 cgcctggggc gagccgagca cggtactgcg cgcgagctgc tcgcgcagcg agcggcgcgc
     5221 catctcgagg accgcttgga gctgcacgta tttcgcgctg ccgagccccg gaatgccgca
     5281 gaagcgcgcg ctgtccgccg cgcacagcgc cgagagcgag ccgaaggcgc tcagtacctc
     5341 gcgcgcgcga tcgaccgcgc ttttgccctt cagtcccgtg cgcaggaata tcgccacgag
     5401 ctcggcatcg gagagggact ccgcgccctt gccgagcagc ttctcgcgcg gccgttcgtt
     5461 gagcggccag tcggtgatcg ccatcgcgct ccatgccatg ctttgcttaa gcgaagcaac
     5521 gcaagtccgg aacaaaacgt ttaccgcgcg tgcgtttgcc gctccctttc tttgcagtta
     5581 cacttcttcc gctccggact gaaagcgcta cgtgactgat cccgctcgca aaaaagtact
     5641 gctcggcctg accggtggca ttgcggccta caaggcggcc gagttgtgcc ggctgctcgt
     5701 caaggacggc atcgatgtcc aggtcgtgat gaccgaagca gcgcgggggt tcattacgcc
     5761 ggccacgatg caggcactgt ccggaaaacc tgtctttacc gacatgtggg acgagcgcgt
     5821 gccggacaac atggggcaca tcgagctctc gcgcggcaag gacgccatcg tagtcgcccc
     5881 cgccaccgcc gacttcatcg cgaaactggt tcacggcctc ggcgacgatc tgctctccac
     5941 gctgtgcctt gcgcgggagt gtccgctcgt cgtagcgccg gcgatgaacc ggcagatgtg
     6001 ggataacgca gcgacacggc gcaacatggc acgactcgca gccgacggcg tggccatcgt
     6061 cggtccggcg agcggcgatc aggcctgtgg cgaagtcggc atggggcgca tggtcgagcc
     6121 ggatgcgatc gcagaggcgg tgcgcgcgct gtggggaccc aaagcactgc gtggcgtgcg
     6181 ggtgatggtg acagccggcc cgacgttcga ggcaatcgac ccggtgcgcg gcatcaccaa
     6241 tcgcagctcc ggcaagatgg gctacgctat cgcccgagct gcgatcgatg cgggcgcgcg
     6301 cgtgacgctg atctccggcc cgactgcgct cgcgacgccc gcaggcaccg aacgcgtcga
     6361 cgtcgtctcc gcagaggaga tgtgcggggc tgtaaagcgc cgcatttccg aggtggacat
     6421 cttcgtcggc gtcgctgcgg tgggcgatta ccggccggtt cgacaaagcg agcacaaaat
     6481 caagaaaagc acgcgcaatc tcacgatcga gcttgagccg aaccccgaca tcctcgccta
     6541 cgtcgccgag cttcccaacc ggccgttctg cgtggggttt gccg
//
LOCUS       DASURG010000496         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1004560, whole genome shotgun sequence.
ACCESSION   DASURG010000496 DASURG010000000
VERSION     DASURG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004560"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFY80_08755"
     CDS             complement(<1..561)
                     /locus_tag="VFY80_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6008039.1"
                     /translation="MSGTEILAILMLVAFFALLMAGVPVAITLATSGFVFGVLGFGTG
                     LFNLLPARIYGVVSNYQWLAIPLFVFMGVMLEKSRLAEDLLDVVGHLAGGLRGGMALG
                     IIGVGVLMGATTGIVGATVITLGLLTLPTLIRRGYHPGVACGAICASGTLGQIIPPSL
                     ILILLADIMGESVGTLFAAAFVPGLVL"
     gene            complement(558..1085)
                     /locus_tag="VFY80_08760"
     CDS             complement(558..1085)
                     /locus_tag="VFY80_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX6008040.1"
                     /translation="MPETRAAGAARCVRAGIERLIEVVGEWTAWISLVIVILMAVNVL
                     LRYGFSVGSVWAQELEWHLLAPLILFGMSYALRHGEHVRVDIVYGRFPLRAKLFVDLV
                     SALLTIAISVLVIWFSLNYVQQSYVIDEGSPDPGGLPHRFLLKALIPVGFALLLLQAI
                     ASALLSFDKLREREL"
     gene            complement(1078..2175)
                     /gene="dctP"
                     /locus_tag="VFY80_08765"
     CDS             complement(1078..2175)
                     /gene="dctP"
                     /locus_tag="VFY80_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX6008041.1"
                     /translation="MKNKRRTFLKGGAVAAAAATTAGFPTIARAQQTFNWKMTSAYPK
                     GAPFYMDGPGSATDLAKRIEAMSNGRLRITVYGAGELIPALEGFDAVRAGTVEMNHAN
                     SYFWTGKTFAAQYFTAVPFGLNFQGMNGWLYDGGGIPLWHEVYAPFNMIAFPCGNTGV
                     QMTGWFKREIKSVKDFNGLKMRIPGLAGKVYATLGVDVKLLPGGEIFPALERGVIDAA
                     EFVGPYQDRRLGLQKAAKYYYTTGWHETATVSELLINRAAWNKLPKDLQAIVENACAA
                     CNVVSEAWCQRTNAEAMEDLVKNQGVIARPLPDDVIKSLRAATAKVLEEAIAKDPVTK
                     KVHTSYMAFKAKYDSWAGYSEAVYHGKIRGA"
     gene            2355..>2889
                     /locus_tag="VFY80_08770"
     CDS             2355..>2889
                     /locus_tag="VFY80_08770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6008042.1"
                     /translation="MISHRHTLLAAICLAWILPGLTGHDPWKPDEAYTFGVVYEMLKG
                     GSWIVPRLAGEVFLHEPPLFHITAAASARLFSPLLPLHDAARLVSGFYMGLTFLFCAL
                     AGRELNGARYGTVAALLLLGCFGLVIRSHQLITDVAALCGFATAHYGFALAIRRAVAG
                     GSWIGVGLGLVFMTQGPS"
BASE COUNT          532 a          957 c          895 g          505 t
ORIGIN      
        1 gaggacgagg ccgggaacga aggcggccgc gaaaagcgtt ccgaccgact cgcccatgat
       61 gtcggcgagg agaatgagga tgaggctcgg cgggatgatc tggccgaggg tccccgaagc
      121 gcagatcgcg ccgcacgcga cgccaggatg gtagccgcgg cgaatcagcg tggggagtgt
      181 caggagccct agcgtaatga ccgtggcgcc aacgataccg gtcgtagccc ccatcagcac
      241 ccccacgccg atgatgccga gcgccatgcc cccgcgtagc ccgcccgcaa gatggcccac
      301 aacgtcgagg agatcctccg cgagccgcga tttttcgagc atcacgccca tgaacacaaa
      361 aagcgggatc gcgagccact gataattgct gaccacacca tagatgcggg cgggcaggag
      421 attgaagagg cccgtgccaa agcccagcac gccgaacacg aagccgctgg tcgcaagcgt
      481 gatcgcaacc ggcacccccg ccatgagcag cgcgaagaac gccaccaaca tcaggatggc
      541 gagaatctcc gtcccgctca cagctcgcgc tcgcgcagtt tgtcgaagga gaggagtgcg
      601 gaagcgatgg cctgcaggag cagcagggcg aagccgaccg gaatgagcgc cttcagcaga
      661 aagcgatggg gaagaccgcc gggatcaggc gagccttcgt cgatcacgta cgactgctga
      721 acgtaattga gcgaaaacca gatcaccaga accgaaatcg cgatcgtgag gagcgcggaa
      781 accagatcga cgaaaagctt ggcgcgcaga ggaaagcgcc cgtagacaat atccacgcgg
      841 acatgctcgc catggcgcag agcataagac atgccgaaga ggatgagcgg cgcgagcaga
      901 tgccactcca gctcctgcgc ccataccgag cccacgctga agccgtagcg cagcagcacg
      961 ttgaccgcca tcaaaatgac gatcacgagc gagatccacg cggtccactc cccgaccacc
     1021 tcgatcaggc gctcgattcc cgcgcgcacg cagcgcgccg cgcccgccgc ccgcgtttca
     1081 ggcaccgcga atcttgccgt gatagacggc ttcgctgtag ccggcccagc tgtcgtactt
     1141 cgccttgaac gccatgtaag acgtgtgcac tttcttggtc accgggtcct tggcgatcgc
     1201 ctcttccaac actttcgcag tcgccgcgcg caggctcttg atcacgtcgt ccggcagcgg
     1261 gcgcgcgatg acaccctggt tcttcacgag gtcctccatc gcctccgcat tggtccgctg
     1321 gcaccacgct tcgctcacga cgttgcacgc ggcgcacgca ttctcgacga tcgcctgcag
     1381 gtctttcggc agcttgttcc atgccgcgcg gttgatgagg agctccgaga cggtggccgt
     1441 ctcgtgccag ccggtggtgt agtagtactt cgccgccttc tggagtccca gccggcggtc
     1501 ctgatagggc ccgacgaatt cggccgcgtc gatgacgccg cgctcgagcg cagggaagat
     1561 ttccccgccc ggcaagagct tcacatccac ccccagcgtg gcgtacacct tgccggcgag
     1621 acccgggatg cgcatcttga ggccgttgaa gtccttgacc gacttgattt ccctcttgaa
     1681 ccagccggtc atctgcacgc ccgtattgcc gcacggaaag gcgatcatgt tgaaaggcgc
     1741 atagacttcg tgccacagcg ggatgccgcc gccgtcgtag agccagccgt tcatgccctg
     1801 gaaattcagt ccgaacggca cggcggtgaa gtactgcgcg gcgaacgtct tgcccgtcca
     1861 gaaataactg ttggcgtgat tcatctcgac cgtgccggcc cgcaccgcgt cgaatccttc
     1921 cagcgcgggg atgagctcgc ccgctccata gacggtgatc cgcaaccgcc cgttcgacat
     1981 ggcctcgatg cgcttggcga gatccgtggc gctgcccggc ccgtccatgt agaacggcgc
     2041 acccttggga tacgcgcttg tcattttcca gttgaatgtc tgctgcgcgc gggcaatcgt
     2101 cggaaaaccg gcagtggtgg cagccgcagc cgccacggcg ccgcccttca gaaatgttct
     2161 gcgcttgttc ttcatggctc cctccgttgg tgtggactgg tgcttcacgc gtgccgcgcg
     2221 tcatatggtc tcagtaccgc gggacggtgc gcaagcgcaa aggccgccgg cgtggttccg
     2281 accgccccgt gcgataatcg caccattcgc ggacaacaag aacgaacctt gatcggcgca
     2341 gccccgcacc gcccatgatc tctcaccggc acaccctgct cgcggcgatt tgcctcgcat
     2401 ggatcctgcc cggactcacc ggacacgacc cgtggaagcc cgacgaggcg tacaccttcg
     2461 gggtcgtgta cgagatgctc aaaggcggct cgtggatcgt tccgcgtctc gccggtgagg
     2521 tgttcctgca cgagccgcct cttttccaca tcaccgccgc ggcgagcgct cgcctgtttt
     2581 ccccgctgct gccgctgcac gacgcggcgc gcctcgtgag cgggttttac atggggctca
     2641 ctttcctctt ctgtgcgctt gccgggcgcg aattgaacgg cgcgcgctac ggtaccgtcg
     2701 cggctttgct gttgctcggc tgcttcgggc tcgtgatccg cagccatcaa ttgattaccg
     2761 atgtcgcggc gctgtgcggc ttcgcgaccg cgcactatgg atttgcgctc gctatacggc
     2821 gtgcggttgc gggcggctcc tggatcggag tggggctcgg gctcgttttc atgacccagg
     2881 ggccgtctg
//
LOCUS       DASURG010000497         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_493874, whole genome shotgun sequence.
ACCESSION   DASURG010000497 DASURG010000000
VERSION     DASURG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493874"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFY80_08775"
     CDS             complement(<1..294)
                     /locus_tag="VFY80_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX6008043.1"
                     /translation="MRFDKFTTKYQQAFADAQSAAVGNDNPYIEPQHLLFALLNQQDG
                     TTASLLGRAGVNAAGLRAGLKSAIDRMPKVEGAGGEVNVSRELANLLNLTDREA"
     gene            644..2242
                     /locus_tag="VFY80_08780"
     CDS             644..2242
                     /locus_tag="VFY80_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX6008044.1"
                     /translation="MVYSQKTPWTLIVGVIMIAYAYAVQSGMMEPVIQYLNTGRYTGE
                     MRVLGYSFGAAAVVVGLWQLLARPREGHLDYYLSTVAGVAFIMLIAFIVKWGLDPQMA
                     KWGRAAQPTLGFNFASVLNLNYVVMGIVAGIVVVNVFKVPSWAENGVRLSRLGLKTGV
                     ILLGTLYSFAELAQLGKLSVLMIAVFVLGSVWLVLLIGVRRNLPNSMTGVMSSGIGVC
                     GVSASVAAAPVVQAKSLEIAYTIGTVLLFGVVCMFVFPIVGKALGMGYVQFGAWAGTG
                     ILNSAQVAGAALAFQPTGIETLKVAEIFNITRVLFLPIIVLWLAIWYVRREEAAARHV
                     QQVNVTEVIVSKFPVFVLGFILMFLLSSTGIFAPARHYQGAYFDNSEKIMVRKDRSGK
                     EVNHYLKEPEIAALRKDLEKIKREDQRAAIQRLIEGRKIMSEEDDAVVRGVINAKIVS
                     KESAAALNRAHRAVRHTAPKIAKFRDLIAWFFTFGLVGLGMQITASSIRQAGGQPLVI
                     GSIVGVIKAVGSLIVVLLFVSETI"
BASE COUNT          412 a          678 c          734 g          472 t
ORIGIN      
        1 cgcttcgcga tcggtcaggt tgagcagatt ggcgagctcc cgagatacat tgacctcgcc
       61 gcccgcacct tcgaccttcg gcatgcgatc aatcgcgctc ttgagtccgg cgcgcaagcc
      121 ggcggcgttg acaccggcgc gtcccagcag ggacgcggtc gtgccgtcct gctggttgag
      181 cagcgcgaag agcagatgct ggggctcgat atacggattg tcattgccga cagcggcact
      241 ctgggcgtcg gcaaacgcct gctgatactt ggtcgtaaac ttgtcgaagc gcatgagcgt
      301 tcgctccaaa aggactcaca tcggatattg ggcaggcaaa ccggatttca agtgcctgaa
      361 aattctacgt tgccgaccgg cacagcggga agtccgctcg attgagaacg cgcgggctgc
      421 ccggcgcttt cgctccccgg cggaaatgcg cgcatgttcc tttggtgaag cacacctatc
      481 aatagattcg tgcctgatat cgaatatttt tatgcgactg tcttcatctg cagcttgact
      541 tgacgcgcac tcccgcctaa cactacgaaa ggcagcggca caagaacgac cgagcgcggc
      601 gtgcaaggcg ccgtaccgtc aacgttgcac agagggggag cacatggtct acagtcagaa
      661 gacgccgtgg acgctcatcg tcggcgtgat catgatcgcg tacgcgtatg ccgtgcaaag
      721 cggcatgatg gagccggtca tccagtacct caacacgggc cgctacaccg gcgaaatgcg
      781 ggtgctgggc tattcctttg gcgccgcggc agtcgtggtg ggattgtggc agctgctcgc
      841 gaggccgcgt gaggggcatc tcgactacta cctctctacc gttgcgggcg tcgccttcat
      901 catgctgatc gccttcatcg tcaaatgggg cctcgatcct cagatggcga agtggggacg
      961 cgccgctcag cccacgctgg gattcaactt tgcgagcgtt ctgaacctga actacgtcgt
     1021 gatgggcatc gttgcgggca tcgtcgtggt gaacgtcttc aaggttccga gctgggccga
     1081 gaacggtgtg cggctgtcgc ggctggggct caagacaggt gtgattctgc tcggcacgct
     1141 ctacagcttt gcggagctgg cgcagctcgg aaagctctcg gtcctgatga tcgcggtgtt
     1201 cgtgctgggg tcggtgtggc tggtgctgct gatcggcgtg cgccgcaacc tgccgaactc
     1261 catgaccggc gtcatgtctt cgggcattgg cgtgtgtggc gtatcggcct ccgtcgcggc
     1321 ggcgcctgtg gtgcaggcga aatcgctcga gatcgcctac accatcggca ccgtgctgct
     1381 cttcggcgtc gtgtgcatgt ttgtattccc gatcgtcggc aaggcgctcg ggatgggcta
     1441 tgttcaattc ggcgcctggg ccggcaccgg cattctcaat tccgcccagg tggcgggcgc
     1501 cgcgctcgcg ttccagccga cgggcatcga aacgctcaag gtcgccgaga tcttcaacat
     1561 cacccgggtg ctgtttttgc cgatcatcgt gctgtggctc gcgatatggt acgtacggcg
     1621 cgaggaggcg gccgcgcgtc acgtgcagca ggtgaacgtc accgaagtga tcgtcagcaa
     1681 gtttccggtc ttcgtcctcg gtttcattct catgttcctg ctctcttcca ccggcatctt
     1741 cgcgcccgcc cggcactatc agggcgccta cttcgacaac agcgagaaga ttatggtgcg
     1801 caaggatcgc tccggcaagg aagtgaacca ctacttgaag gagcccgaga tcgcggcgct
     1861 caggaaggac ctcgaaaaga tcaagcgcga ggatcagcgt gcggcgatcc agcgcctcat
     1921 cgagggcagg aaaatcatgt ccgaagagga cgatgccgtc gtccgcggcg tgatcaacgc
     1981 gaagattgtc tcgaaggagt ccgcggcggc gctcaatcgg gcgcaccggg cggtacgtca
     2041 caccgcgccg aagatcgcga agttccggga tctgatcgcg tggttcttca ccttcgggct
     2101 ggtgggactg gggatgcaga tcaccgcatc ctccatccgc caggccggcg ggcagccgct
     2161 cgtgatcggc tccatcgtcg gcgtcatcaa ggcggtgggc tcgctcatcg tcgtgctgct
     2221 cttcgtttcc gaaacgatct aggaggctac cgtggaagaa aaattcgatc ggggctcggc
     2281 gctgaccata ttcaag
//
LOCUS       DASURG010000498         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1106564, whole genome shotgun sequence.
ACCESSION   DASURG010000498 DASURG010000000
VERSION     DASURG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106564"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFY80_08785"
     CDS             complement(<1..1097)
                     /locus_tag="VFY80_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX6008045.1"
                     /translation="MGMTAADKILARASGLRDTRAGDIVYPVPDLVIIHDGVVMGAKQ
                     ELDAIAIDRLFDPARIFMVTDHDVVYTSERAAARGAFNRKAAKAWGVVHFYDAGQGGH
                     GHIFPIERGHVLPGTFYFDNDRHCTNAGGIGALAIRVGTEMSRVLATGTVWTMVPRTV
                     KLTIEGRMKAGVYARDLGFEIGRRLRTGSEFGVDIDYRVLEFAGELDQFSLAARVSLC
                     STPTEMRAIGVFFPPSEEIVAAARERAQRPFTPAYPDRDATYEAEIRMDVGALEPQVA
                     LPGGPQNAVNLAEVAGTRVDHAFLGSCGSGMYEDLVTAGGILKGRRLAPGVRFIVTPG
                     SEDSTRRMRREGLLDIFHDAGALVMPAGCGV"
     gene            1176..2165
                     /locus_tag="VFY80_08790"
     CDS             1176..2165
                     /locus_tag="VFY80_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008046.1"
                     /translation="MKSVRASALVLTVAVLVFAAERAPAQWKPEKPVEIIAPSGPGGT
                     TDRTARLVAKILVQNKLVDVPVNVVNKPGGSGTIGLNYLNQHPGDGHHIIIATTGSIS
                     NHILGLIPYNHTAFTPLAMLFEEYLGVNVSAGSAIKSGADLVARLKKDPQSVSFGVST
                     SLGGGNHTTLTTCLRAGGVDIKRLKTAIFPGGAATTTALLGGHVDAINTGLGNMVAHL
                     RSGKLRTLAISAPKRMWGDFASVPTWREQGVPADSSSWRGLMGPQGMSAQQIAYWDKV
                     FAALAQTDEWKQDLEANFWVNGYVGAQAARKRLDDEYAEYKAVLTELGLAKIK"
BASE COUNT          421 a          708 c          721 g          379 t
ORIGIN      
        1 cacacaccgc agcccgccgg catcaccagc gctccggcat cgtggaaaat gtcgagcagg
       61 ccttcccggc gcatgcgccg ggtggagtct tcagaaccgg gcgttacgat aaaccgcaca
      121 ccgggcgcga gccgccgccc cttcagaatg ccgccggccg taaccaggtc ttcgtacatc
      181 cccgatccgc atgagccgag aaaggcgtga tcgactctag tgccggcgac ctcggccaga
      241 ttcaccgcgt tttgcggacc accggggagc gcgacctgcg gctccagcgc gcccacgtcc
      301 atccggattt ccgcctcgta cgtcgcgtcg cgatccggat aggcgggcgt gaacgggcgt
      361 tgcgcgcgct cgcgcgctgc cgcgacgatt tcttccgagg gcgggaaaaa aacgccgatc
      421 gcacgcatct cggtcggcgt gctgcagagc gagacgcgcg cagcgagact gaactggtcg
      481 agctcgccgg cgaattccag cacgcgatag tcgatgtcca cgccgaattc gctgccggtt
      541 cgcaaccgcc tgccgatctc aaaaccgaga tcgcgcgcgt agacaccggc tttcattcga
      601 ccttcgatcg tgagcttcac cgtgcgcggc accatggtcc ataccgttcc ggtcgcgagt
      661 acgcgtgaca tttcggtacc gacccggatc gcgagcgcgc cgatgccgcc ggcgttggtg
      721 caatgacgat cgttgtcgaa gtaaaacgtg cccggaagga catggccgcg ctcgatcggg
      781 aagatatggc cgtgcccgcc ctgtcccgca tcgtagaaat gcacgacgcc ccacgccttt
      841 gctgccttgc ggttgaacgc gccgcgcgct gccgcacgtt cgctggtgta gaccacatcg
      901 tggtcggtca ccatgaagat tcgcgcaggg tcgaacagcc ggtctatcgc gatggcatcg
      961 agctcctgct ttgcgcccat caccacaccg tcgtgaatga tgacgaggtc gggaaccgga
     1021 tagacgatgt cgccggcacg cgtgtcgcgc aggccggatg cgcgcgcgag aattttgtcc
     1081 gcggcggtca ttcccatggc cgaagtatat cctcctgctg ccggatggag tgctatcatc
     1141 cgactggctc gatcgcaatg gggatggaga aaaaaatgaa atcggtgcgg gcatccgctc
     1201 tggtgttgac ggtggcagtg ctggttttcg cagcggagcg cgcgccggcg cagtggaagc
     1261 cggagaagcc ggtggagatc atcgcgcctt cgggtccggg cggcacgacg gatcgcaccg
     1321 cgcggctcgt ggcgaagatc ctggtgcaaa acaaactggt cgatgtgccg gtaaacgtgg
     1381 tgaacaagcc tggcggcagc ggtacgatcg gcctcaacta tctcaatcag catcccggcg
     1441 acgggcacca tatcatcatc gccacgaccg gctcgatcag caatcacatc ctgggtctca
     1501 tcccgtacaa ccacacggca ttcacgccgc tcgcgatgct gtttgaggaa tacctcggcg
     1561 tgaacgtaag cgcgggctcg gcgatcaaga gcggcgccga tctcgttgcg cggctgaaaa
     1621 aggatccgca gtccgtgagc ttcggcgttt cgacgagcct cggcggaggc aatcacacga
     1681 cgctcaccac gtgcctgcgc gcgggcggcg tggacatcaa gcggctcaag accgcgatct
     1741 tcccgggcgg agccgcgacg accaccgcgc tcctcggcgg ccacgtcgat gccatcaaca
     1801 ccgggctggg caacatggtc gcgcacctga ggagcgggaa gctgcgcaca ctcgccattt
     1861 cggcaccaaa gcgcatgtgg ggtgatttcg catccgtgcc gacgtggcgc gagcagggcg
     1921 tgcccgcaga ttcgtcgagc tggcgcgggc tcatgggacc gcaaggcatg tcggcgcagc
     1981 agattgcata ctgggacaag gtgttcgcgg cgctcgcgca gacggatgag tggaagcagg
     2041 acctcgaagc caacttctgg gtgaacggct atgtcggtgc gcaggctgcg cgcaagcgcc
     2101 tcgatgacga gtatgccgag tacaaggcgg tcctgaccga gctcggcctc gcgaaaatca
     2161 aatagacggc acgccggggc taaagtgccg cgctcctcgg cgtgatacag tattcgatct
     2221 tcttgttgg
//
LOCUS       DASURG010000499         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_392490, whole genome shotgun sequence.
ACCESSION   DASURG010000499 DASURG010000000
VERSION     DASURG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392490"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFY80_08795"
     CDS             complement(<1..494)
                     /locus_tag="VFY80_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX6008047.1"
                     /translation="MKHELPPLPYAMDALAPHVSKETLEFHYGKHHQAYVTNLNNLIA
                     NTEYDKMTLEEIVKKAPPGGVFNNAAQVWNHTFFWSCMKPGGGGEPTGAVKAAIEKKW
                     KSVDEFKKQFQASAVGNFGSGWTWLVKKPDGSVDIVNTSNAGTPLTTSDKALLTIDVW
                     EHAY"
     gene            complement(624..1598)
                     /gene="ybiB"
                     /locus_tag="VFY80_08800"
     CDS             complement(624..1598)
                     /gene="ybiB"
                     /locus_tag="VFY80_08800"
                     /inference="COORDINATES: protein motif:HMM:NF006005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HEX6008048.1"
                     /translation="MKSYAEYLLLLDANGGLASDRDLELLAGAMLDGGIPDVELGALL
                     ATVRSRSASVHFLRALLAALDTRVTRWRSEERSAPVVIGCYGGAADIPNLSALIGLLL
                     ARFDVPVLMHGPLHAERGLSTALVLRELGIMPCTQKQQVAQELRTHRIAFVPDALLAP
                     GLASLLALRARLGPIPMLVTAARLVDPFDCGALAVAGAEGARELELMRIALAGNGARA
                     LLLRASGGEAFANPLVRPRMEFWTCGEPHVLFEEDASQTARAAALPAPLDVRSTAAWI
                     RQACEGTRAIPTPIVNQLAACLYAAGYCEDFNQAKALAALAKAGRHVA"
     gene            complement(1857..>2323)
                     /locus_tag="VFY80_08805"
     CDS             complement(1857..>2323)
                     /locus_tag="VFY80_08805"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6008049.1"
                     /translation="YASLYRALYGVSALGLRYFNVYGPRQDAASPYAGVISRFSANLA
                     AGLPLVVFGDGQQTRDFVFVGDVARANVAALESRYQGVINVGTGKSVSLLALIDALGR
                     CAGCDPQVRFEAPVPGDIRDSAMRPERMRDVLGFVPATGLEDGLAHLLAYPG"
BASE COUNT          394 a          785 c          788 g          356 t
ORIGIN      
        1 tagtacgcgt gctcccacac gtcgatggtg agcaaggcct tgtcgctcgt ggtgagcggc
       61 gtgccggcgt tgctggtgtt gacgatgtcg accgagccgt ccggcttctt cacgagccag
      121 gtccagcccg agccgaagtt gccgaccgcg gaggcctgga actgcttctt gaactcgtcg
      181 accgacttcc acttcttctc gatcgccgcc ttcacggcgc ccgtgggctc gccgccaccg
      241 cccggcttca tgcagctcca gaaaaaggtg tggttccaca cctgcgcggc attgttgaag
      301 acgccgcctg gaggcgcttt cttcacgatc tcctcgagcg tcatcttgtc gtattcggtg
      361 ttcgcaatga gattgttcag gttggtgacg tacgcctggt gatgcttgcc gtaatggaac
      421 tcgagcgttt ccttggagac gtgcggagca agcgcgtcca tggcgtacgg cagcggcgga
      481 agctcgtgct tcatcgggtc ttctcctgtt ggcgcggatg gaaccgggca ttctagccaa
      541 acggatggag cgcaacaacg ggatccggta tttggcatcc ggcccggcgc gcaatccgaa
      601 cgccacacga gctcgcgggt tcgtcatgcg acatggcgcc ccgccttggc gagcgctgcc
      661 aatgccttgg cctggttgaa atcttcgcaa tagcccgcgg cgtacaggca ggccgcgagc
      721 tgattgacga tcggcgtcgg aattgcacgc gtgccttcgc atgcctgtcg aatccacgct
      781 gcggtcgagc gcacgtcgag cggcgcagga agcgcggctg cccgcgccgt ttgcgaggcg
      841 tcctcctcga acaggacgtg cggctcgccg caggtccaga actccatgcg cgggcgaacg
      901 agcgggttcg cgaacgcttc gccgccgctc gcccgcagca gcagcgctcg cgccccgttc
      961 cccgccagcg cgatgcgcat gagctcgagc tcgcgcgcgc cttccgcgcc ggccacggcg
     1021 agcgcgccgc aatcgaacgg atcgaccaga cgcgcggcgg ttacgagcat ggggatcggc
     1081 cccaggcgcg cgcgcaaggc gagcagcgac gcgagacctg gcgcaagcag tgcgtcgggt
     1141 acgaatgcga tccggtgcgt cctcagctcc tgagccacct gctgcttctg cgtgcacggc
     1201 atgatgccca gctcgcgcag aacgagcgcc gtcgagagcc cgcgctccgc gtgcagcggc
     1261 ccgtgcatca gcaccggcac gtcgaaacgc gcgagcagca gcccgatgag cgcagagagg
     1321 ttcgggatat cggccgcgcc cccatagcaa ccgatcacca ccggagcgct ccgttcctcg
     1381 ctgcgccagc gcgtcacgcg cgtgtccagc gccgcgagca aggcccgcag gaaatgcacc
     1441 gatgccgagc ggctgcgcac cgtggcgagc agcgcaccga gctcgacgtc cggtatgccg
     1501 ccgtcgagca tcgcgccggc gaggagctcc aggtcgcgat ccgaagcgag cccgccgttc
     1561 gcatccagca agagcaggta ctcggcgtaa gacttcatgc gagcgcgtcc gattggtcgg
     1621 gtgccggcga tggcagcaag cgttatgcca agccgcacag atgtggatcg ggcgcaaaac
     1681 gcactgcgcc gcaggcatgg acgagcacat cgtggaccat gcacagcccg tagccgccgc
     1741 aatcgagcgc gcggccgcgc ggcgccgcca cattcgggtg cgtcgcccgg aacccggcgc
     1801 attgaaatgg tgccaagcga ccccgtgccg cccgggcgcg gcaatgggcg cgcgtgtcag
     1861 ccggggtatg cgagaagatg cgcgaggccg tcttcgagcc cggtcgccgg cacgaagccc
     1921 agcacgtccc gcatgcgctc gggccgcatg gccgaatcgc gtatgtcgcc gggcacgggc
     1981 gcctcgaagc gaacctgggg atcgcacccg gcgcagcggc cgagcgcatc gatgagcgcg
     2041 agcaggctca cgctcttgcc ggtgccgaca ttgatcacgc cctggtagcg ggactcgagg
     2101 gcggcaacgt tcgcgcgtgc gacatcgccg acgaacacga agtctcgggt ctgctgcccg
     2161 tcgccgaaaa ccacgagcgg caatccggcg gcgagattgg ccgagaagcg gctgatgacg
     2221 cctgcataag gcgaagcggc gtcctgccgc gggccataga cattgaagta gcgcaggccg
     2281 agcgcgctca cgccgtacag cgcgcggtat agcgacgcgt act
//
LOCUS       DASURG010000500         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1311771, whole genome shotgun sequence.
ACCESSION   DASURG010000500 DASURG010000000
VERSION     DASURG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311771"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VFY80_08810"
     CDS             complement(<1..107)
                     /locus_tag="VFY80_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6008050.1"
                     /translation="MRAIRNIAIIAHVDHGKTTLVDQLLRQSGTFAAHQ"
     gene            365..1225
                     /locus_tag="VFY80_08815"
     CDS             365..1225
                     /locus_tag="VFY80_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6008051.1"
                     /translation="MTPFELLEPRSLRKAAALLASDGGVVRPMGGGTALMLMMKMGVL
                     RPTRLVSLRGIEKRYSRISAVKEGGLRIGALATLSEIERAPAVRREAPVIAQTLRTLA
                     NVRVRNVATLGGHLAHADPHLDLPPVLIALGASVEISGANGVRTVPLEQLYSGYLETT
                     LAPGELIAEVRIPRQRPRYAAYLKCTTRSADDWPALGVAVLVDREVDRVRDARMVVSA
                     ATDTPTRLKETEKALCGTDVSPDALRRLGDIAASEVTLMADAHGSAPYKKELLRVYVA
                     RAIRSALEQR"
     gene            1226..>2880
                     /locus_tag="VFY80_08820"
     CDS             1226..>2880
                     /locus_tag="VFY80_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6008052.1"
                     /translation="MAGVGSYGETGGAGEAPGAVGRSVPRLEARAKVTGRAEYVHNLV
                     LPGMLHGKIFRSSVAHGRIKRIDVGGARAVKGVYRVVTAEDIRSLIPDPYYGPAFHDQ
                     PILAIDKVHYAGEPVGVVLAADPHVAEEAAQQIVAEYEELPAVYDEVEAMTSRAVVHE
                     KLKPAGTFPDLKHLQGRRDTNIALDFHLRRGDAARAFAEADRVFEHTFRTQQVLHVPL
                     EPFVTVVEPMESGITIHTASQSPSFVRIEMARLLGWPENRVRVTVPYLGGGFGAKLYI
                     KLEALVTALALLTRRPVKIALSMEEQFYTLTKHAATLRIKSAVTKEGRISARECEVWW
                     NGGAYADIGPRITQKSGFTAPGPYDIEHIAIDSYALYTNLPPAGALRGFGIPQLVWAY
                     ESHTDMMARELGIDPVEFRRRNILRDGRPQATGTIMEDAAIGAVLERLAERMKWAQPF
                     DRGTGTVKRGRGIAIGFKASISPTTSVAILNVIADGSCTLYLSTVDMGQGSDTAMAQI
                     VAEVLNIPAEEVKVVHPDTDVTPYDMATLGSRSTFHAGNAVRLA"
BASE COUNT          504 a          915 c          982 g          479 t
ORIGIN      
        1 tgctggtgcg ccgcaaacgt gcccgactga cgcagcagct gatcgacgag cgtcgtcttg
       61 ccgtgatcga cgtgggcaat gatggcgatg ttgcgtatgg cgcgcatggg ttcccgagcg
      121 aaaagcgcgc gattgtagca cctgcggacg tcgagggaca ggcggcgttt cacttcgtct
      181 gtctgggtat tcgcgtcgca acggcatgcc gcgcgaattg attttgggcg tcgttctcag
      241 tagagtctcc tcacgcaggg ccgacttcga tcgatgcggt cctgcatgcg ggctcaacaa
      301 cgttccggaa gggcctgcgc tgccgcgccg ccgtattttc agagcacgac gagcgaacgc
      361 cgaaatgaca ccgtttgagc tgctcgagcc ccgttcgctg cgcaaagccg cagcgctgct
      421 cgcgtccgat ggcggtgtgg tgcgtccaat gggcggcggg acagcgctca tgctcatgat
      481 gaaaatgggc gtgctgcggc caacgcgcct cgtgagcctg cggggaatcg agaagcgcta
      541 ttcccgcatc agcgcggtga aggagggcgg actgcgtatc ggggcgctcg ccacgctttc
      601 cgagatcgaa cgggcgccgg ccgtgcggcg cgaagcgccg gtcatcgcgc agacgctgcg
      661 cacgctcgcc aacgtgcggg tgcgcaatgt cgcaacgctc ggcggacatc tcgcacacgc
      721 cgacccgcac ctcgatctgc cgcccgtgct catcgcgctg ggcgcgagcg tggaaatctc
      781 gggcgcaaac ggcgttcgca cggtgccgct cgagcaactg tattcaggtt acctcgagac
      841 gacgcttgcg cccggcgaat tgatcgccga agtgcgcatc ccgcgccagc ggccgcgata
      901 cgccgcttac ttgaagtgca cgacgcgctc ggcggacgac tggcccgccc tcggcgttgc
      961 cgtcttggtc gaccgcgagg tcgatcgtgt gcgggacgcg cgtatggtcg tgagcgcggc
     1021 aaccgatacg ccgacacggt tgaaagagac cgagaaggcg ttgtgcggaa ccgatgtttc
     1081 acccgatgcg ctgcgccggc tcggcgatat tgccgcgagc gaagtgacgc tcatggccga
     1141 tgcgcacggg tcggcgccat acaagaaaga actgctgcgg gtgtatgtcg cccgcgcgat
     1201 ccgcagtgcg ctggagcagc ggtagatggc gggggttggc tcatacggag agacgggcgg
     1261 ggcgggcgaa gcgccgggcg ccgtcggccg ttcggtgccg cggctggagg cgcgcgcgaa
     1321 ggtcaccggg cgcgccgagt atgtgcacaa cctcgtgctg cccggaatgc tccatgggaa
     1381 gatcttccgc agcagcgtcg ctcatgggcg catcaagcgc atcgacgtgg gcggcgcgcg
     1441 ggcggtgaag ggcgtgtatc gcgtcgtcac ggcagaggac atccgctccc tcatacccga
     1501 tccgtactac gggccggcgt tccacgacca gccgatcctc gcaatcgaca aggtgcacta
     1561 tgcgggcgag ccagtcggtg tcgtgctggc cgccgatccg cacgtggcag aggaggcggc
     1621 gcaacagatc gtcgccgagt acgaggaact gcccgcggtg tacgacgaag tcgaagcgat
     1681 gacttcgcgc gcggtggtac acgaaaagct gaaaccggcc ggcacctttc ccgatctgaa
     1741 gcacctgcag ggcaggcgcg acaccaatat cgcgctcgat tttcatttgc ggcgggggga
     1801 tgccgcgcgc gcgttcgccg aagcggaccg cgtgttcgag cataccttcc gcacgcaaca
     1861 ggtgctgcac gtgccgctcg aaccgttcgt caccgtggtg gagccaatgg aaagcggtat
     1921 caccattcat accgcatccc agagcccttc gttcgtgcgc atcgagatgg cgcggctcct
     1981 gggctggccg gaaaatcgcg tgcgggtcac ggtgccgtat ctgggcggtg gctttggcgc
     2041 gaagctctat atcaagctcg aagcgctggt gacggcgctc gccctgctta cgaggcgtcc
     2101 ggtcaagatc gcgctttcaa tggaggagca gttctacacc ctcaccaagc acgcggcgac
     2161 gcttcgtatc aagagcgcag tcacgaaaga gggtcgtatc agcgcgcgcg agtgcgaagt
     2221 gtggtggaac ggcggcgcct acgccgacat tggcccgcgc atcacgcaga agtccggctt
     2281 caccgcgccg ggaccgtacg acatcgagca cattgcgatc gactcatacg ctctctatac
     2341 caatctgccc ccggccggcg cgctgcgcgg tttcggcatt ccgcagctcg tatgggccta
     2401 cgaaagccat accgacatga tggcgcgcga gctcgggatc gatccggtgg aattccgccg
     2461 ccgtaacatc ctgcgcgacg gccggccgca ggcgaccggg acgatcatgg aggatgccgc
     2521 gatcggtgcg gtgctcgagc gcctcgccga gcgcatgaag tgggcgcagc cgttcgatcg
     2581 aggtaccggc accgtcaaac gcggacgcgg aatcgcgatc ggcttcaagg cctcgatctc
     2641 gcccaccacc tcggtggcga tcctcaacgt catcgccgac ggcagctgca cgctctatct
     2701 ctccaccgtc gacatgggac agggctccga caccgccatg gcgcagatcg tggccgaggt
     2761 gctgaacatt ccggccgagg aggtgaaggt ggtgcatccg gacacggatg tgacgcccta
     2821 cgacatggcc acgctcggct cgcgctccac cttccacgcc ggcaacgcgg tgcggctggc
//
LOCUS       DASURG010000501         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1517068, whole genome shotgun sequence.
ACCESSION   DASURG010000501 DASURG010000000
VERSION     DASURG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517068"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VFY80_08825"
     CDS             complement(<1..1628)
                     /locus_tag="VFY80_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX6008053.1"
                     /translation="MPADSHQSERQSEFVRNLHAFAQQHRASHWSGTFAEFLEKVVPG
                     NTRGIARNSHQYVWDMIRWQGSEIDQEGHSRYKLFADELYGIDDSLERLADYFKAASA
                     GSEVGRRLLLMLGPPSGGKSSIVILLKRGLEEYSHTDEGAIYAIQGCPVNESPLHLVP
                     YTLRPQFREIYGVDIVGDLCPLCRARLSEFRGNSPQAGEGKLSGDFMQMPVQRIFISE
                     AGRVGIGTYAPHDPTTADIADLVGSVDLSKVAEYGDEGDPRAWSWSGAMYASSRGVLE
                     MIEILKVKREFLYLLLTLTQEKNVKVSRFPLIYLDEAILAHTNLAEFRRFLQESENEA
                     LLDRMVIIQVPYTLSYKDEARIYKKLISSAVAFKEVHLDPHALHTAAVFAILTRLHEG
                     EEKETEFSKKVRVHAGEDVEGVNQADAERIRARMPDEGLGGVSPRFVINALSNAIIQG
                     DAKSLTTMEMLLALKDAIESDARIDPKKKRKWVDFLVIARKDFYNRWVKEDVHKALFV
                     SFEQEAQDLLDKYLDEVEATLDNRPVKDPMTKEER"
BASE COUNT          463 a          778 c          763 g          482 t
ORIGIN      
        1 cgctcctctt tggtcatcgg atcctttacc ggccggttgt ccagcgtcgc ctcgacctcg
       61 tcgagatact tgtccagcag gtcctgcgcc tcctgctcga acgagacgaa gagtgccttg
      121 tgcacgtcct ccttcaccca gcggttgtaa aagtccttgc gcgcgatgac caggaaatcc
      181 acccacttgc gcttcttctt ggggtcgatg cgcgcatccg attcgatcgc atccttgagc
      241 gccagcagca tttccatggt ggtgaggctc ttcgcatcgc cctggatgat ggcgttcgaa
      301 agcgcgttga tcacgaagcg ggggctcacg ccgcccaggc cttcgtccgg catgcgcgcc
      361 cgaatgcgct cggcgtcggc ctggttgacg ccttccacgt cctcaccggc gtgaacacgc
      421 accttcttgg aaaattccgt ctccttttcc tcgccctcgt gcaggcgcgt gaggatggcg
      481 aacacggctg cggtgtgcag agcgtgcgga tcgaggtgga cttccttgaa cgcgaccgct
      541 gacgaaatca atttcttgta gatgcgggct tcgtccttgt aactcaacgt atacggcacc
      601 tggatgatga ccatccggtc gagcagggcc tcgttttcgc tctcctgcag gaagcgccga
      661 aattccgcga gattggtgtg ggcgaggatg gcctcgtcca gatagatgag cggaaaccgc
      721 gacaccttga cgttcttctc ctgggtcagc gtgagcagca gatacagaaa ctcgcgcttc
      781 accttcagga tctcgatcat ctccagcacg ccgcggctcg aagcgtacat cgcgcccgac
      841 cacgaccacg cccgcggatc gccttcatcg ccgtattccg cgaccttcga aagatcgaca
      901 gagcccacga ggtcggcaat gtcggccgtg gtcggatcgt gaggggcgta ggtcccgatg
      961 cccacccgtc cggcttccga gatgaatatg cgctggaccg gcatctgcat gaagtccccg
     1021 gagagcttgc cttcaccggc ttgaggactg ttgccgcgga actcggaaag ccgggcgcgg
     1081 cacagcgggc agagatcgcc gacgatgtcc acgccgtaga tttcgcgaaa ctgcggacgc
     1141 agggtgtacg gcacgagatg gagaggcgac tcgttcaccg gacagccctg tatggcgtag
     1201 atcgcgccct cgtcggtgtg gctgtactcc tcgagcccgc gcttgagcag aatgacgatg
     1261 ctcgatttgc cgcccgaggg cggcccgagc atcagcagca ggcgccggcc gacctcggag
     1321 cccgcgctcg ccgccttgaa ataatcggcg agccgctcca gcgagtcatc gattccgtac
     1381 agttcgtctg cgaaaagctt gtaccggctg tggccttcct ggtcgatctc gctgccttgc
     1441 cagcggatca tgtcccatac gtactggtga ctgttgcggg cgatccctcg cgtgttgccc
     1501 ggcaccactt tctccaggaa ttccgcgaac gtgcccgacc aatggctggc gcggtgctgc
     1561 tgtgcgaatg cgtggagatt tctgacgaac tcgctctggc gttcggactg gtgggaatcg
     1621 gccggcatcg ttttacctcc cgctacagtg gtatgggcct cacaacaatg gacatggcgg
     1681 caaattcatg gttccgtcga cgcttgtaag caacgggcgc gtatcccgtt tgcggtgcac
     1741 acaactataa gtccggttcc gctcctttaa cagcgtaccg tttctcatgc ggcgatcggc
     1801 tcgaaaacaa cgctcgcgtt cgcgaacacc cgtcaaaaaa acgtgatcta tcacggctat
     1861 gcgggctgtc gccggtccgt gccccgcgct ctcaaagcgg ctccacgcat tgcggttgac
     1921 aagcgaaaag agcgcacgtt ccagcacggc catcatagcg caatcattga gcgacccggc
     1981 aaccggttgc gtcaaggtag aatcgcctgt tgtcgcgccg tttcgccggg cgggcgacgc
     2041 aaggccacac ggcgctcatt accagcggag cacccctcgc gggcgcgata gggccggact
     2101 gcgaccgggt ccccggggca aagtaagtct gcgcgggaga tgacgcaggc tggcgccgca
     2161 ccggtcgtca tcgtgccccc gtcatggaaa agcgagcgct ccgatggcac gtccgtccgc
     2221 ggtccgcgcg caggaacgtc ctgaaaccat cggcgataca tcggttctaa cgggactgac
     2281 gtttgcggct gcatgccggg gaaaggaggc gaaaggtgac acttactcag gggcagagga
     2341 ctgcactcac cggggcgctc gacaagctcg agaccgaggt gcgggagaaa attcgcgagt
     2401 ccatgccgca gctcgcggac cagaaataca tcgacctggt gggtgtggtg tacgacaccg
     2461 gagacgaggc atcggcgacc atgcag
//
LOCUS       DASURG010000502         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_188643, whole genome shotgun sequence.
ACCESSION   DASURG010000502 DASURG010000000
VERSION     DASURG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188643"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFY80_08830"
     CDS             <1..475
                     /locus_tag="VFY80_08830"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX6008054.1"
                     /translation="LERARQASIEAMHIQVSGRPADLSTREMLAALDSADIDLIALAG
                     YLRLIPPAVVKRYPQRIVNIHPALLPRFGGRGMYGLHVHRAVLAAGEKESGATVHFVN
                     EQYDQGDIIAQARVPVLPGDTPESLGARVLAVEHELLPDALEKLARSILAEELNA"
     gene            468..2021
                     /gene="purH"
                     /locus_tag="VFY80_08835"
     CDS             468..2021
                     /gene="purH"
                     /locus_tag="VFY80_08835"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX6008055.1"
                     /translation="MPRALLSVSDKTGIVEFGRALSALGWEIVSTGGTARALREGGVR
                     VLDVSEVTGHPEVMDGRVKTLHPAVHAGILARRSHSADVETLERLGYRGIDLVAVNLY
                     PFHEAVAGNVPLAEAMDEVDIGGPTMLRAAAKNHADVLVVVDPRDYERVLGAVEAGDT
                     GLELRRALARKVFEHTATYDRAIADYFARPDGSEPAEFPSRITLALQLVQSLRYGENP
                     DQPAAFYREQNAPAASVPDLEQLHGKELSFNNLLDVEAAVTAVSAWHADDDVACVIIK
                     HTTPCGIALAPNPEDAYQRALACDPVSAFGGIVAFNRPLTGSVASQLAETFLEIVIAP
                     DFEANALEQLRRKKNLRLIKLPVTVAGRAELDFKRVRGGMLVQARMRMEFPEGDWKVV
                     TQRTPEEDELRDLRFAWRVCASVKSNAIVLAARQRTLGIGAGQMSRVDSSRIAVLKAQ
                     DQNAELRGAVLASDAFFPFRDGVDAAAAAGIRAVIQPGGSVRDAEVIEAANAHDMAMI
                     FTGRRVFRH"
     gene            2058..2140
                     /locus_tag="VFY80_08840"
     tRNA            2058..2140
                     /locus_tag="VFY80_08840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2091..2093,aa:Ser,seq:tga)
     gene            complement(2145..>2395)
                     /locus_tag="VFY80_08845"
     CDS             complement(2145..>2395)
                     /locus_tag="VFY80_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008056.1"
                     /translation="CRESRVEQCFRDFHHVVRTGFNLQVGGIVEHVETGMLREQLPAL
                     GNELERLGRGTQAGASDDDRPRIDRNSRFVGQRAKRTV"
BASE COUNT          429 a          756 c          768 g          442 t
ORIGIN      
        1 gctcgagcgt gcgcgccagg ccagcattga ggccatgcat attcaggtga gtggccgccc
       61 ggccgatctg tcgacgcgcg aaatgttggc tgcactcgat tccgcggaca tcgatctgat
      121 cgcgctcgcc ggctatctgc gactgatccc gccggccgtg gtcaagcgct acccgcaacg
      181 catcgtcaac atccacccgg cactgctgcc tcgcttcggc ggacgcggta tgtatggcct
      241 gcacgtgcac cgcgccgtgc tggccgccgg tgaaaaggaa agcggcgcga ctgtgcactt
      301 cgtgaacgag cagtacgacc aaggcgacat tatcgcgcag gcccgcgttc cggtcctgcc
      361 cggtgacacg ccggagtcac tgggcgcgcg tgtgctcgcg gtcgaacatg aactactgcc
      421 cgacgcgctg gagaaactgg cgcgcagcat cctggccgag gagcttaatg cctagagccc
      481 ttctgagtgt gtcggacaag accggtatcg tggagttcgg acgtgcactg tccgcgctcg
      541 ggtgggagat cgtgtccacc ggcgggaccg ctcgcgcgct gcgcgagggc ggcgtgcgcg
      601 ttctcgatgt gagtgaagtg accggccatc ccgaggtgat ggatggccgc gtcaagacgc
      661 tgcaccccgc agtgcacgcc ggcatcctcg cccgccgctc gcattcggcc gacgtggaaa
      721 cgctcgaacg actcggctac cgcggcatcg atctcgtggc cgtgaatctc tacccgtttc
      781 atgaagccgt ggccggcaac gtgcccctgg ccgaagccat ggatgaagtc gacatcggcg
      841 gcccgaccat gctccgtgcg gccgcgaaga accacgccga tgtattagtc gtggtcgatc
      901 cgcgcgacta tgagcgtgtg ctgggtgctg ttgaagcggg tgacacggga ctggaactgc
      961 ggcgcgcgct ggcacgcaag gtgttcgagc ataccgccac ctacgaccgg gccattgccg
     1021 actactttgc tcggcccgat ggcagcgagc cggcggagtt cccgagccgg atcacactgg
     1081 cattacagct cgtacaatca ctgcggtacg gtgaaaaccc ggaccagccg gccgcgtttt
     1141 atcgcgagca gaacgctccc gctgcttctg tgcccgatct cgagcagctg cacggcaagg
     1201 agctgtcctt caacaatctg ctggatgtgg aagccgcggt gacggccgtc agcgcgtggc
     1261 acgccgatga cgacgtcgcg tgtgtcatca tcaaacacac gacgccctgt ggcattgcgc
     1321 tcgcgcccaa tccggaagac gcgtatcagc gcgcgctcgc ctgcgatccg gtctccgcgt
     1381 tcggcggcat tgtcgccttc aaccgcccgc tcacgggcag cgttgccagc cagttagcgg
     1441 aaacattcct ggagatcgtg atcgcgccgg atttcgaggc gaatgcactg gagcaactgc
     1501 gcagaaagaa gaatctgcgc ctgatcaagc tgccggtcac ggttgccggt cgcgctgagc
     1561 tggacttcaa acgcgtacgt ggcggcatgc tggtgcaggc gcgcatgcgg atggagtttc
     1621 cggaaggcga ttggaaagtg gtgacgcaac gcacaccgga agaggacgag ttgcgggacc
     1681 tgcgctttgc ctggcgcgtg tgtgcgtcgg tgaaatcgaa tgccatcgtc ctggccgcgc
     1741 gccagcgcac gctgggcatt ggcgccggcc agatgagtcg cgtcgattct tcccgtatcg
     1801 ccgtactgaa ggcgcaggat cagaacgcgg agctgcgcgg cgccgtgctc gcgtcggacg
     1861 cgttcttccc cttccgcgac ggcgtggacg cagccgcagc cgccggcatc cgcgccgtca
     1921 ttcagcccgg cgggtccgtg cgcgatgctg aagtcattga agccgccaat gcgcacgaca
     1981 tggcgatgat cttcaccggc agacgcgttt tccggcattg aaagaattgc tgatttgcgc
     2041 gatatttccg aggtcgagga gggttggcag aacggctatt gcaccggtct tgaaaaccgg
     2101 cgtccgcaag gacttccgag ttcgaatctc gggccctccg ctcttcaaac ggttcgttta
     2161 gcgcgttgcc ctacgaaacg actgttgcga tcgatgcgcg gacgatcgtc atcagaggct
     2221 ccggcctggg taccacggcc gagacgctcc agctcatttc ccaaagccgg caattgttcg
     2281 cgcagcatcc cggtttcaac atgctctacg attccaccga cctgcagatt gaatccagtc
     2341 cgaacgacat gatgaaagtc gcggaagcat tgttcgaccc tgctttcgcg gcatt
//
LOCUS       DASURG010000503         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_904134, whole genome shotgun sequence.
ACCESSION   DASURG010000503 DASURG010000000
VERSION     DASURG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904134"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..437
                     /locus_tag="VFY80_08850"
     CDS             105..437
                     /locus_tag="VFY80_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008057.1"
                     /translation="MKKIIAGCAIVAGLLPSAPVAAAPEDVRIYEPGMLTRDRYEIVT
                     RLWVESWRSAFQVPTHATRHEALSEMRAQAARRGANALTNVTCLTDDAPFPGAKPHFC
                     YALAIRLK"
     gene            complement(444..1289)
                     /locus_tag="VFY80_08855"
     CDS             complement(444..1289)
                     /locus_tag="VFY80_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6008058.1"
                     /translation="MSESAARLGQWIGKKESRRGIATAWPAAALAAMLDHPDPEPPIG
                     SAIAPGGHWLYLLDTTPQAELGPDGLPQRGGFLPPVGLPRRMWAGGRIEFRSAITIGS
                     DIARESEVISVEEKQGKSGALVFVTVQHTISCDERAAVVEEHDIVYREAAKPGDVPAQ
                     GKPAPAKAAWQRQLIAGPVMLFRFSALIFNANRIHYDMDYCRENGYPGLVVHGPLQTV
                     LLLDLCRRHETRGVKRLEYRALHPIYHTERFTLNGELQAGATRAQLWTANAAGHYAMT
                     ATAYF"
     gene            1392..2141
                     /locus_tag="VFY80_08860"
     CDS             1392..2141
                     /locus_tag="VFY80_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6008059.1"
                     /translation="MEWLADPHAWLALVTLAALEIVLGVDNIIFITILVGRLPAHQRA
                     RARTLGLGFAMLTRILLLLSLAWIMTLTQPLFTLLGKEISGRDLILIGGGLFLLWKSV
                     HEIHNALEGEEVEQHAAAPAVATFGSIIVQIAIIDIVFSLDSVITAVGMVDEVSIMII
                     AIVIAVGVMMFAAGPIGTFVDRHPTIKMLALSFLILIGIALIGEGWDLHIPKGYIYFA
                     MAFSVAVEMLNLRIRARRGAPVHLRRHMPNE"
     gene            complement(2143..>2651)
                     /locus_tag="VFY80_08865"
     CDS             complement(2143..>2651)
                     /locus_tag="VFY80_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008060.1"
                     /translation="LLRELVESIRHEEGEPALPYYFIGHSAGGQLLSRLAAFAPGEAR
                     RIVIANPSTYVWPTREVRFPFGFGGLPAPLSGDDTLRRYLAQPVTIYLGSEDIAHDAS
                     LNVSADAMRQGTNRYERGLRVFEAARALTQKNGWPFAWRLVEAPGVGHSARAMYANPA
                     ADAALSPD"
BASE COUNT          448 a          827 c          865 g          511 t
ORIGIN      
        1 gtcgtgggcg aggaggagac cgagggtctc gatacggtcc tccataacga aaaaggctat
       61 aacctgtaga atcggctcgg gtaacttcaa ccgtcgaggc gccgatgaag aagatcatcg
      121 caggctgcgc gattgttgcc ggactgctcc cgagcgcgcc agtcgcggcc gcgcccgagg
      181 acgtgcgcat ctacgagccc ggcatgctga cgcgcgatcg ctacgagatc gtgacgcggc
      241 tctgggtaga gtcgtggcga agcgcgtttc aggtgcccac tcacgccacg cggcatgagg
      301 cgctctccga gatgagggct caagccgcgc ggcgcggtgc gaatgcgctg accaacgtga
      361 cgtgccttac cgacgacgcg ccttttccgg gcgcgaagcc gcacttctgc tacgcgctcg
      421 cgattcgcct gaagtaggac gatctagaag tacgcagtag ccgtcattgc gtaatggccg
      481 gcggcattgg ccgtccacaa ctgcgctcgc gtggcgcccg cctgtagctc gccgttcagc
      541 gtgaaccgtt cggtgtgata gatgggatgc agcgcgcgat actcgagccg ctttacgccg
      601 cgcgtttcgt gccggcggca gaggtcgagc agcagcacgg tctgcaacgg tccgtgcacg
      661 acgaggccgg gatagccgtt ttcgcggcag taatccatgt cgtaatggat gcggttcgca
      721 ttgaaaatga gcgcggaaaa gcggaacagc atgacgggcc ccgcaatgag ttgccgctgc
      781 catgctgcct tggccggcgc cggcttgcct tgtgccggca cgtcgcccgg cttcgcggct
      841 tcgcggtaga cgatgtcatg ctcttctaca accgcagcgc gttcgtcgca cgatatcgtg
      901 tgctgtacgg tgacgaagac gagtgcgccg cttttgccct gtttctcctc gaccgagatg
      961 acctcggact cacgcgcgat gtccgaccct atggtgatcg cgctcctgaa ttcgatgcgg
     1021 cctccggccc acatgcggcg cggtaacccg accggcggca ggaaaccgcc cctctgggga
     1081 aggccgtctg gccccagttc cgcctgcggc gtggtatcga gcagatacag ccaatgccct
     1141 ccgggcgcga tcgcgctccc gatggggggc tcgggatcgg ggtgatcgag catcgctgcg
     1201 agcgcagccg ctggccacgc cgtcgcgata cccctgcggg attctttctt tccgatccac
     1261 tgtccaagtc gcgcagctga ttcgctcatg tcgatggatt cctcggggag agtcgtttgc
     1321 gaccggagcg ggtgaatgta gcatagcgcg cccgcctata atgccgggcg ttttcctgat
     1381 gggagtgact catggaatgg ctcgctgatc cgcacgcctg gcttgcactc gtcacgctgg
     1441 cggccctcga aatcgtgctc ggcgtggaca acatcatctt catcacgatc cttgtggggc
     1501 ggctaccggc ccatcagcgg gcccgggcgc gcacgctcgg cctgggattt gccatgctca
     1561 cccgcatcct cctgctcctg tcccttgcct ggatcatgac actcacgcag ccgctcttta
     1621 ccctgctcgg caaggaaatt tccggccgcg atctcattct gatcggaggc ggccttttcc
     1681 tcctgtggaa gagcgtgcac gaaatccata acgcgctcga aggcgaggaa gtggagcagc
     1741 acgccgcggc gccggcggtg gcgacgttcg gctcgatcat cgtccagatc gccatcatcg
     1801 acatcgtctt ctcgctggat tccgtgatta ccgcagtcgg catggtggac gaagtatcga
     1861 tcatgatcat tgcgatcgtc atagcggtcg gcgtcatgat gttcgcggcc ggtccgatcg
     1921 gaactttcgt ggaccggcac cccaccatca agatgctggc gctgagcttt ctcatcttga
     1981 tcggcattgc gctcatcggt gaaggctggg atctgcacat accgaagggc tatatctatt
     2041 tcgcgatggc gttttccgtg gcggtggaga tgttgaatct tcgcatccgc gcaagacgcg
     2101 gcgcgccggt gcacctgcgg cggcatatgc cgaacgagtg agtcagtcgg gtgagagtgc
     2161 cgcgtctgcg gcggggttcg cgtacatcgc gcgcgcgctg tggcccacgc cgggcgcctc
     2221 caccagacgc catgcgaagg gccagccgtt cttctgcgtg agcgcgcgcg cggcttcgaa
     2281 gacgcggagc ccgcgctcgt agcgattcgt gccttggcgc atggcatcgg cactgacgtt
     2341 gagcgaggca tcgtgtgcga tgtcttcgga gccgagatag atcgtcaccg gctgcgcgag
     2401 atagcggcgc agcgtgtcgt ctccgctgag cggcgcgggc agtccgccaa agccgaacgg
     2461 gaagcgcact tcgcgcgtcg gccacacgta tgtggacggg ttggcgatca cgatgcggcg
     2521 cgcttcccca ggggcaaacg cggcgaggcg gctgagaagc tgaccacccg ccgaatgtcc
     2581 gatgaaataa tacggaagcg caggctcgcc ttcctcgtgc cgaatgctct ccaccagttc
     2641 gcgcaggagc g
//
LOCUS       DASURG010000504         4561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_293077, whole genome shotgun sequence.
ACCESSION   DASURG010000504 DASURG010000000
VERSION     DASURG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293077"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFY80_08870"
     CDS             <1..937
                     /locus_tag="VFY80_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008061.1"
                     /translation="LLAAAVPIAAPGAERYPDRPIRLIVGFPPGGAADILGRMAGQQL
                     GDALGQQVVVDNRGGAGGLIATEIAVRGSPDGYTLLFSSIPHVINPHLYRKVNYDAIR
                     DFVPVIQFVSVPLMMACAPKLAAQSVKDLIALAQAKPDGINYASAGAGSSSHLAMELF
                     KSMAGVRMTHIPYKGTGPLIADLMTGQVNVTIASAVPLAPQVKSGRLRGLAVTSPKRS
                     AAFPELPAIAETVPGYEVINWFGIFAPRGTPASIVSRINGALNKALKSGDLVKLLNAR
                     GADAVGGTPDAFARVVRDDYAKWGRVVKESGARVD"
     gene            953..2053
                     /locus_tag="VFY80_08875"
     CDS             953..2053
                     /locus_tag="VFY80_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008062.1"
                     /translation="MALLPDPRRYAACATDEVLVQWAQKSAAGDDEARSALLRSVRER
                     LAAGRDDELLGALRRVPSAMVGACLWELMCRAVDEGDAADHRGVVARIFAFPVLLVAA
                     ARESAVLPGTLPDVDEIRTLLERHGALGATRNFGLGDALVTKDALEAVKPSVVYRWTR
                     DWSGPPRDLPPEAIRLRQRGREEVHLRFIVGAGITPAEAPSLVETASNIGAWGLPLAR
                     ALVRQLAQPGLEVLALPRPPLSLLRAPLAGRWAEIETAWHLFVSNTLREFRSSVGEPT
                     VRVSAHEDPGGMGEVRVSMSSALDDTVLEGFRWPLHPFDDLRRIETMIAELLRECRVN
                     DVRFVPERLPERLRSGHLFLRAGDTEERGAAH"
     gene            2053..3042
                     /locus_tag="VFY80_08880"
     CDS             2053..3042
                     /locus_tag="VFY80_08880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX6008063.1"
                     /translation="MSAMPHPYANLTPDCMLDAVESCGYRCDGRQLALNSFENRVYLV
                     YLEEGAPLVAKFYRPERWSDGAILEEHEFALELEAREIPVVAPLRCNGRTLHEHAAYR
                     FALYPRRPGRSPELENRETLTWLGRFIARMHAVGAMKRFAHRPAIDLESFGSEPSRFV
                     IENGFIPPDLSETYRTVVEDVLSRVARCESRAGDVTTLRLHGDCHAGNILWTEAGPHF
                     VDLDDARTGPAVQDLWMCLSGDRRAMEMQLAALIEGYREFYDFDPAELALIEALRTLR
                     MVHYAGWLARRWSDPAFPANFPFFNTQRYWQDHVLALREQAALLDEAPLELLL"
     gene            3057..3875
                     /locus_tag="VFY80_08885"
     CDS             3057..3875
                     /locus_tag="VFY80_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6008064.1"
                     /translation="MLDLMLPLAVVYGCVVGLVFLFQSHLVYYPQIGRELSATPQAYG
                     LRYEDVEIATEDGERIFGWWVPVAEARGTALIFHGNAGNISHRLDYLRMFHRLRYSSF
                     IIDYRGYGRSSGSPSEAGTYRDAAAAWEHLTGKRGIAAGDIVLFGESLGGAVASWLAA
                     RTTPRALVMHSTFTSAPDLGAQVYWFLPVRLISRFRYDSLSRLAEVKAPVLIIHSRED
                     DIVPYAHGERLYQAAPEPKQLLTLRGGHNDAFVFVRDEWVRALDEFLARHAPSR"
     gene            complement(3896..>4561)
                     /locus_tag="VFY80_08890"
     CDS             complement(3896..>4561)
                     /locus_tag="VFY80_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6008065.1"
                     /translation="IIDSHGLMLAYLGDAEAHGAALALASPVVGGAVTDDGILLEVGG
                     KEAMDLVCRTVVNSAGFHAQSVARSVEGIAASTIPPTYYAIGHYYTLAGKAPFRRLVY
                     PVARQDWLGVHVTVDLGGQVKFGPDFNWIDRIDYRFDESREAAFYEAIRRYYPGLRDG
                     ALQPGYTGIRPKIAGPGAPAPDFVIQGPLEHDVHGLVNLYGIESPGLTASLAIADHVA
                     ALV"
BASE COUNT          735 a         1470 c         1527 g          829 t
ORIGIN      
        1 gcttctggcg gcagccgtgc cgattgcggc gcctggggcg gagcgctacc ccgaccgccc
       61 gatccggctc atcgtgggat ttccgcccgg cggtgccgcg gatattcttg gacgcatggc
      121 gggccagcaa ctgggggacg cgctcggcca gcaggtggtc gtcgacaacc gcggcggcgc
      181 gggcggcctc atcgccaccg agatcgccgt gcgcggatcg ccagacggct acacgctgct
      241 cttttcatcc attccgcacg tcatcaaccc gcatctctat cgcaaggtga attacgatgc
      301 gatccgggat ttcgtgccgg tgatccagtt cgtcagcgtg ccgctcatga tggcgtgcgc
      361 gccgaagctt gccgcgcaat cggtgaagga tctcatcgcg ctggcccagg caaagccgga
      421 tggcatcaat tacgcttccg caggcgccgg ttcgtcgagc cacctggcga tggagctctt
      481 caagtccatg gcgggtgtac gtatgacgca cattccgtac aagggcaccg gtccgctcat
      541 cgcggacctc atgacgggcc aggtgaacgt caccatcgcg agcgcggtac cgcttgcgcc
      601 gcaggtgaaa tcggggcgat tgcgtgggct cgccgtgacc agtccgaagc gctccgccgc
      661 tttccccgag ctgcccgcga ttgcggagac cgtgcccgga tacgaagtga tcaattggtt
      721 tggcatcttc gcgccccgcg ggacgcccgc gtcgatcgtc tcgcgcatca acggtgcgct
      781 gaacaaagcg ttgaagtctg gcgatctcgt caagctgctc aacgcccgcg gggcggacgc
      841 ggttggcggc actcctgatg cgtttgcacg cgtcgtcagg gacgactacg ccaaatgggg
      901 gagggtggtg aaggagtccg gcgcgcgggt cgattgacgc ccgctatcgc gcatggcgct
      961 gttacccgat ccgagacgct atgccgcctg cgcgaccgat gaggtgctcg tgcagtgggc
     1021 gcaaaagagt gcggcaggcg acgatgaggc gcgttccgcg ctgctccgct cggtaaggga
     1081 gcggctcgct gccggacgcg acgacgagct gctcggagcg ctgcgtcgcg taccgtcggc
     1141 aatggtgggc gcgtgcctgt gggaactcat gtgccgcgcg gtggacgaag gcgatgccgc
     1201 agaccaccgt ggcgtcgtcg cacggatctt cgcgttcccc gtgctgctcg tcgccgcggc
     1261 gcgtgagtcg gccgttctgc cgggaacgct gcccgacgtg gacgaaatcc gaactttgct
     1321 ggaacggcac ggcgcgctcg gcgcgacacg taacttcggg cttggcgatg cgctggtgac
     1381 aaaggatgcg ctcgaagcgg tgaagccgag cgtcgtctat cgttggacgc gcgactggtc
     1441 gggcccgccg cgcgatcttc cgcccgaggc gattcgactg cgacagcggg gtcgtgaaga
     1501 ggtgcatctg cggttcatcg tgggtgccgg aatcacgccc gccgaagcac cgtcgctcgt
     1561 ggagacggca tccaatatcg gcgcgtgggg cctgccgctc gcccgggcgc tcgtgcggca
     1621 gctggcgcaa cccgggctcg aagtgctcgc gttgccacgg ccgccgttga gccttctgcg
     1681 cgcgccgctc gccggacggt gggccgagat cgagacggca tggcatcttt tcgtgagcaa
     1741 tacgctgcgt gagttccgct cgtcggtggg cgagccgacc gtcagggttt ccgcgcatga
     1801 agatccaggc ggcatggggg aagtgcgggt cagcatgagt tctgcgctcg acgatacggt
     1861 gctcgaagga tttcgctggc cgctgcatcc cttcgatgac cttcgccgca tcgagacgat
     1921 gatcgccgaa ctgctgcgcg aatgccgggt gaacgatgtg cgattcgttc cggaacggct
     1981 ccccgagcga ctgcgctcgg gccatctttt cttgcgcgca ggcgacacgg aggagcgcgg
     2041 tgccgcgcac tgatgtctgc gatgccccac ccgtacgcca acctgacccc cgattgcatg
     2101 ctcgacgcgg tggagagctg tggctatcgc tgcgacggca gacagcttgc gctgaacagc
     2161 tttgagaacc gcgtctatct cgtgtatctc gaagagggcg cgccgctggt ggcgaagttc
     2221 taccggcccg agcgctggtc ggacggcgcg attctcgaag aacacgaatt tgcgctcgaa
     2281 ctggaggcgc gcgaaattcc ggtcgtcgct ccgcttcggt gcaacggccg cacgctccac
     2341 gagcacgccg cctaccgctt cgcgctctat ccgcgccggc ccggacgtag ccccgagctg
     2401 gaaaatcgcg aaacgctgac gtggctcggg cggttcatcg cgcgcatgca cgcggtgggc
     2461 gcgatgaagc gctttgccca ccgacccgcc atagacctcg agagcttcgg aagcgagccg
     2521 tcgcgtttcg tgatcgagaa cggattcatc ccgcccgacc tgagcgagac ctatcgcacc
     2581 gtggtggagg acgtgctgag tcgggtggcg cgatgcgaat cgcgcgcggg agacgtgact
     2641 acgctgcgcc tccacggcga ctgccatgcc ggcaacatcc tctggaccga agcagggccg
     2701 cacttcgtgg acctcgatga cgcgcgcacc ggtcccgccg tgcaggatct gtggatgtgc
     2761 ctgtccggag atcgccgtgc gatggaaatg caactcgctg cgctgatcga aggctatcgg
     2821 gaattctacg acttcgatcc ggccgagctc gcgctgatcg aggcgcttcg cacgctgcgc
     2881 atggtgcact atgcaggctg gctcgcgcgg cgctggagcg atcccgcgtt cccggcgaat
     2941 ttccctttct tcaatacgca gcgctattgg caggatcacg tgctcgcact gcgcgagcag
     3001 gctgcgctgc tcgacgaggc gccgctcgag ctcctgctgt gagacccgtg agagcaatgc
     3061 tcgacctgat gctccccctc gcggtcgtct acggatgcgt ggtcggactc gtgttccttt
     3121 tccagtcgca tctcgtgtat tacccgcaga tcggtcgcga gctgagcgcg acaccgcaag
     3181 cctatggcct gcgctatgaa gacgtggaga tcgcaaccga agatggggaa aggatcttcg
     3241 gctggtgggt cccggtggcc gaggcgcgcg gcacggcgct catttttcac ggcaacgccg
     3301 gcaacatctc gcatcggctc gattacctgc gcatgttcca ccgcctgcgc tattcgagct
     3361 tcatcatcga ctaccggggc tacgggcgaa gctcgggttc accttcagaa gcggggacct
     3421 accgcgatgc cgcggccgcg tgggagcatc tcactggcaa gcgcggcatt gcggcgggcg
     3481 atatcgtgct gtttggcgaa tcgctgggcg gcgcggtcgc ttcctggctt gccgcccgca
     3541 cgacgccgcg cgcgctcgtc atgcactcca cgtttacttc cgcacccgat ctcggcgcac
     3601 aggtgtactg gttcctgccc gtgcgcctca tcagccggtt tcggtatgac agcctctcgc
     3661 gcctcgccga ggtgaaggct ccggttctca tcatccacag ccgcgaggac gacatcgtgc
     3721 cttatgccca tggcgagcgg ctttatcagg cggcccccga accgaagcaa cttctgactt
     3781 tacgtggcgg gcacaacgac gcctttgtct tcgtgcgcga tgaatgggtg cgcgcgctcg
     3841 acgaatttct ggcgcgccac gcgccttcgc ggtaacagca aaggggacgg ccgggctata
     3901 ccaacgccgc cacgtggtct gcgattgcga gcgacgcggt gagtccgggc gattcaatcc
     3961 catagaggtt gacgaggccg tgaacgtcgt gctcgagcgg gccttggatc acaaagtcgg
     4021 gcgcgggggc gccgggcccg gcaatcttcg gccggatgcc ggtatacccg ggctgcaatg
     4081 ccccgtccct gagtccgggg taatagcggc gaatcgcttc gtagaacgcc gcctcccggc
     4141 tttcatcgaa gcgatagtcg atgcggtcga tccagttgaa atccgggccg aacttgacct
     4201 gtcctccgag atccaccgtc acgtgcacgc cgagccagtc ctggcgcgcg accggataga
     4261 cgaggcgccg gaacggcgcc ttgccggcga gcgtgtagta atgaccgata gcgtaatacg
     4321 tgggcggaat cgtcgacgcc gcaatcccct cgaccgagcg tgccacgctt tgcgcgtgaa
     4381 accctgcgga attcaccaca gtgcggcaga cgagatccat cgcttctttt ccgccgactt
     4441 cgagcaggat gccgtcgtcc gttacggcac ccccgactac cgggctcgcg agcgccaggg
     4501 cggctccgtg ggcttcggca tcgcccaggt aggcgagcat gagcccgtga ctgtcgatga
     4561 t
//
LOCUS       DASURG010000505         6829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_598733, whole genome shotgun sequence.
ACCESSION   DASURG010000505 DASURG010000000
VERSION     DASURG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598733"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFY80_08895"
     CDS             <1..767
                     /locus_tag="VFY80_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6008066.1"
                     /translation="GDRAILKGIDMRISRGKVVAILGTSGSGKTTLLRMLGGQLRPDA
                     GYVKVAGQIVHTLDDAELYALRRQMGMMFQMGGLFTDMSVYENIAFPMRERTDLPEPV
                     IRDLVLMKLHAVGLSGAHRLMPGELSGGMARRVALARAIALDPMLIMYDEPFSGLDPI
                     SLNVIANLIRRLNDALGVTSIVVTYDVVESLKIVDYVYFISDGVVAAEGTAQEMMASD
                     DPFVRQFVHALPDGPVAFHYPRNPYDVDLRLLGAGA"
     gene            complement(728..1312)
                     /locus_tag="VFY80_08900"
     CDS             complement(728..1312)
                     /locus_tag="VFY80_08900"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6008067.1"
                     /translation="MTTIHTIGHGNRPLEELIARLRDAGIGRLVDVRAFPGSRRHPHF
                     ARQALEKALPQAGMEYVWEGSALGGRRRPAPDSANTALRNESFRAYADHMMGAEYQVA
                     LARLIAHSNERSTAIMCAERLPWHCHRHLISDSLVARGVTVLHIVSEAPPRAHAMSPL
                     ARVEGAALVYDRGSVPELDLDGQAPAPSKRRSTS"
     gene            1402..1590
                     /locus_tag="VFY80_08905"
     CDS             1402..1590
                     /locus_tag="VFY80_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX6008068.1"
                     /translation="MGIEVGGIFGLLILIADIWAIINIAQSGASTGSKVLWIVLVIVL
                     PVLGLIIWLLLGPRTARA"
     gene            complement(1625..2362)
                     /locus_tag="VFY80_08910"
     CDS             complement(1625..2362)
                     /locus_tag="VFY80_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX6008069.1"
                     /translation="MAIFLYTDFGTGDIYVGQVKAALHHHAPGIPVIDLLHQAPAFNP
                     RANAHLLAALTRHLPHRAVVMAVVDPGVGSARRPIAVRAANRWFVGPDNGLLSVLAAR
                     ARQVEIYTILWRPAALSSSFHGRDLFAPFAGMLAQEALPADAMAVQSELDVRLGGDDL
                     AEIVYIDHFGNAMTGLRAENVARQRALMVDSRTIRYAQVYSEVDPGELFWYENSIGLV
                     EIASNAASAADALQLRVGQPVLLAQTD"
     gene            complement(2372..3076)
                     /locus_tag="VFY80_08915"
     CDS             complement(2372..3076)
                     /locus_tag="VFY80_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6008070.1"
                     /translation="MLNDLELTGRARTHVVQRDDLNAAVHPEALAAFLAMKAEAARAG
                     FDIEIVSAFRDFAAQQRIWDMKFRGERPLYDAQGKVRNHAELSPAELIEAIACWSAVP
                     GGSRHHWGSEIDVIDRASVPAGERVHLLPSETEPGGVFHALHQWLDENMARYGFYRPY
                     RTYRGGVFPEPWHLSFAPVSTLALERLTPQVFAETVRESDILGRDLVLERIEAFYRRY
                     LANVDAPEFPARRAPV"
     gene            complement(3196..3612)
                     /gene="rplQ"
                     /locus_tag="VFY80_08920"
     CDS             complement(3196..3612)
                     /gene="rplQ"
                     /locus_tag="VFY80_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX6008071.1"
                     /translation="MRHRHGLRKLNRTSSHRLAMLRNMTNSLLRHEVIKTTLPKAKEL
                     RRVVEPIITLGKKPSLANRRLAFDRLRDRDNVVKLFDELGPRYAKRNGGYLRILKFGF
                     RKGDNAPMAYVELLDRPEPTDETEAGKETAAAQSAA"
     gene            complement(3622..4602)
                     /gene="rpoA"
                     /locus_tag="VFY80_08925"
     CDS             complement(3622..4602)
                     /gene="rpoA"
                     /locus_tag="VFY80_08925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX6008072.1"
                     /translation="MQSSAFLKPRIIDVQNISPYHAKLTMEPFERGYGHTLGNALRRT
                     LLSSMPGYAPTEVKISGVLHEYSTLDGVQEDVVDILLNLKGIVLKLHNRDEATLNLKK
                     SGEGVVTAGDIESTHDVEIVNPDHVIAHLASGGKLDMQIKVEQGRGYIAATTRRSIDE
                     EGRNVGSILLDASFSPVRRVSYSVESARVEQRTDLDKLIMDIETNGAIDPEEAVRYAA
                     RVLVEQLSVFADLKGMPTQIEEKKATQIDPILLRPVDDLELTVRSANCLKAENIYYIG
                     DLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEKV"
     gene            complement(4624..5250)
                     /gene="rpsD"
                     /locus_tag="VFY80_08930"
     CDS             complement(4624..5250)
                     /gene="rpsD"
                     /locus_tag="VFY80_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX6008073.1"
                     /translation="MARNLDARCRQCRREGEKLFLKGEKCFTDKCAIERRNYPPGQHG
                     QKNTRLSGYGVQLREKQKVRRTYGLLERQFRRGYAEASRRKEVTGEALLQILESRLDS
                     VAYRMGFGASRTEARQIVRHDGILVNGKRVNIPSFQCRPGDVVEVAQKAKDQLRIKAA
                     AEAAESRGYPEWVEVDTKALKGTFKARPQRSELPSTINESLVVELYSK"
     gene            complement(5266..5655)
                     /gene="rpsK"
                     /locus_tag="VFY80_08935"
     CDS             complement(5266..5655)
                     /gene="rpsK"
                     /locus_tag="VFY80_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6008074.1"
                     /translation="MAKASTRVRKKVKKNVAEGIAHVHASFNNTIVTITDRQGNALAW
                     ATCGSNGFKGSRKSTPFAAQVAAEHAGRLAQESGVKNIEVRIKGPGPGRESAVRALNA
                     CGFKITSISDVTPVPHNGCRPPKKRRI"
     gene            complement(5668..6045)
                     /gene="rpsM"
                     /locus_tag="VFY80_08940"
     CDS             complement(5668..6045)
                     /gene="rpsM"
                     /locus_tag="VFY80_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX6008075.1"
                     /translation="MARIGGVNIPNHQHASIALTAIYGIGRSRANMICTAAGVNGSTK
                     VKDLSDSEMDKLREQLTKFTVEGDLRREVSMNIKRLIDLGTWRGKRHRAGLPVRGQRT
                     RTNARTRKGPRKAAVKIIKPTTV"
     gene            complement(6109..6222)
                     /gene="rpmJ"
                     /locus_tag="VFY80_08945"
     CDS             complement(6109..6222)
                     /gene="rpmJ"
                     /locus_tag="VFY80_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX6008076.1"
                     /translation="MKVLASVKRMCRKCKIIRRKGVVRVICSDPRHKQRQG"
     gene            complement(6248..6466)
                     /gene="infA"
                     /locus_tag="VFY80_08950"
     CDS             complement(6248..6466)
                     /gene="infA"
                     /locus_tag="VFY80_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX6008077.1"
                     /translation="MAKEETIQMQGEIVETLPNATFRVKLENGHIVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLTRGRIVFRAK"
     gene            complement(6467..>6829)
                     /gene="secY"
                     /locus_tag="VFY80_08955"
     CDS             complement(6467..>6829)
                     /gene="secY"
                     /locus_tag="VFY80_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070586.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6008078.1"
                     /translation="FFCYFYTALVFNPKETAENLKKSGAFVPGIRPGDQTARYIEKIT
                     MRLTLTGSIYVTLVCLLPEFLIVWKNVPFYFGGTSLLIIVVVTMDFMAQVQAYVMSHQ
                     YESLLKKANFKGGVFPTR"
BASE COUNT         1141 a         2191 c         2155 g         1342 t
ORIGIN      
        1 acggcgaccg cgcaatcctg aagggcattg atatgaggat ttcgcgcggc aaggtcgtgg
       61 cgatcctcgg aaccagcggc tcgggcaaga ccacgctgct ccgcatgctg ggggggcagc
      121 ttcgaccgga cgcgggctac gtcaaggtcg cgggccagat cgtgcacacg ctggacgacg
      181 cggagctcta cgcgctgcgg cgccagatgg gcatgatgtt ccagatgggc ggcctgttca
      241 ccgacatgtc cgtatacgag aacatcgctt tccccatgcg cgagcgcacc gaccttccgg
      301 agccggtgat ccgcgatctc gtgctcatga aactgcacgc ggtggggctg agcggcgcgc
      361 atcgcctgat gcccggcgag ctttccggcg gcatggcacg ccgcgtcgcg ctcgcgcgcg
      421 cgattgcgct cgatccgatg ctcatcatgt acgacgagcc cttctccggg ctcgatccca
      481 tctcgctcaa cgttatcgcc aacctgatcc ggcgcctgaa cgatgcgctc ggcgtaacat
      541 ccatcgtcgt cacctatgac gtggtggagt cgctcaagat cgtcgattac gtctacttca
      601 tttccgacgg cgtggtggca gcggaaggca ccgcgcagga gatgatggct tccgacgacc
      661 cgttcgtgcg ccagttcgtg cacgcgctcc cggacggacc ggtcgcgttc cactacccgc
      721 gcaatcccta cgacgtcgac ctgcgtttgc ttggggcagg cgcctgaccg tcgagatcca
      781 actccggcac gctcccccgg tcgtagacca gcgccgcacc ttcgacgcga gcgagcgggc
      841 tcatcgcatg cgcgcggggc ggtgcctcgg acacgatgtg caggaccgtc acgccgcggg
      901 ccaccagaga gtcggaaatg agatggcggt gacagtgcca gggcagtcgt tccgcacaca
      961 tgatcgccgt gcttcgctcg ttcgaatgtg caatcaggcg cgcgagcgcc acttggtact
     1021 ccgcgcccat catgtgatcg gcgtaggcgc ggaagctctc attgcgcagt gccgtgttgg
     1081 ctgagtccgg cgccggccgg cgacggcctc cgagtgccga gccttcccag acgtactcca
     1141 ttcccgcctg gggaagggcc ttttcgagtg cctggcgcgc gaaatgcgga tggcgtcggg
     1201 aacccggaaa tgcgcgcacg tcgaccaggc gcccgatgcc cgcgtcgcgc aagcgcgcga
     1261 tgagctcctc gagtggccgg ttgccatgac cgatggtgtg gattgtcgtc atgcggtgac
     1321 agccagtgcc gcgcgggaac aaaagacgcg cacgacccga ccaattgatg cgttccccgt
     1381 catagacaag gagtgatggc catgggcatc gaggtaggcg ggatcttcgg tttgctcatc
     1441 ctgatcgcgg atatctgggc gatcatcaat attgcgcaga gcggcgcatc gaccggctcc
     1501 aaagtcctct ggattgtgct cgtcatcgtg ctgcccgtgc tcggcctcat catctggctg
     1561 ctgctcggac cccgcacggc tcgggcgtag cgacttccct tgcggcggtt tctgccgtga
     1621 tccgtcaatc tgtctgggcc aggagcaccg gctggccgac gcgaagctga agcgcgtccg
     1681 cggcgcttgc ggcgtttgac gcgatctcca cgagcccgat gctgttttcg taccagaaaa
     1741 gctcgcccgg atccacctcg gagtagacct gcgcatagcg gatcgtccgg ctgtccacca
     1801 tcaaagcgcg ttggcgggcc acgttttccg cgcgcaagcc cgtcatcgcg ttgccgaagt
     1861 ggtcgatgta gacgatctcc gcgagatcat ctcctccaag acgtacatcg agttcactct
     1921 gcaccgccat ggcgtccgcg gggagcgcct cctgcgccag catgccggcg aagggggcaa
     1981 agagatcgcg cccgtgaaag gacgaggaca gcgcagcagg ccgccataag atggtgtaga
     2041 tctccacctg ccgggcgcgt gcggcgagca ccgacaagag cccgttatcg ggccccacaa
     2101 accagcggtt ggcggcgcgt acggcgatcg gccggcgggc actgcccacg cccggatcca
     2161 cgaccgccat caccaccgcc cggtgtggca ggtgccgcgt gagtgcggcg agcaggtggg
     2221 cattggcgcg cgggttgaat gcgggcgctt ggtgcaggag gtcgattacc ggaatgccgg
     2281 gcgcatggtg gtgcagcgca gccttaacct ggccgacgta gatgtcgccc gtaccgaagt
     2341 cggtataaag gaatatcgcc atcggacgcc gttaaaccgg tgcgcgtctt gcaggaaact
     2401 cgggggcatc cacgttcgcg aggtagcgcc gatagaacgc ctcaatgcgc tcgagcacga
     2461 ggtcgcggcc gaggatgtcg ctctcacgca ctgtctccgc gaagacctgc ggtgtcaggc
     2521 gctcgagcgc cagcgtcgaa accggcgcga agctcagatg ccacggctcc ggaaagacac
     2581 cgccccggta ggtccggtac ggacgataga agccgtagcg cgccatgttt tcgtcgagcc
     2641 attggtggag cgcgtgaaat acgccgcccg gctccgtttc ggacggcagc agatgcacgc
     2701 gctcgcctgc gggcacgctg gcacggtcga tcacatcgat ctcgctgccc cagtgatggc
     2761 ggctgccgcc cggcacggcg ctccagcagg cgatcgcctc gatcagttcc gcgggagaaa
     2821 gctcggcgtg attgcggacc ttgccctgcg catcgtagag cgggcgctcg ccgcgaaact
     2881 tcatgtccca gatgcgctgc tgcgcggcaa aatcccggaa ggcgctcacg atttcgatgt
     2941 cgaatccggc gcgcgcggct tcggctttca tcgcgaggaa cgcggcgagc gcctcggggt
     3001 gcacggcggc attaaggtca tcgcgctgca cgacgtgcgt tcgcgcgcgg cccgtgagct
     3061 cgagatcgtt cagcatgcgc ggattgtaaa tcgtggcggc tgcccggcgc caagcagccg
     3121 ggtgtaccat gcgccgaccg acaaaaaagc cggcctgcgg ccggctttcc gacaacgagc
     3181 caacaatcgc ggtcgctagg ccgcggattg cgcggcggcc gtctccttgc ccgcctcggt
     3241 ctcgtccgtg ggctcggggc gatccagcag ctcgacgtac gccatcggcg cattgtcgcc
     3301 cttcctgaac ccgaacttga ggatgcgcag atagccgcca ttccgcttcg cgtagcgtgg
     3361 accgagctcg tcgaagagct tgaccacgtt atcccggtcg cgcaggcggt cgaacgcgag
     3421 ccggcggttg gcaagcgagg gcttcttgcc gagcgtgatg atcggctcca ccacacgcct
     3481 cagctccttc gccttcggca gcgtcgtctt gatgacctcg tgccgcaaca gcgagttcgt
     3541 catgttgcgc agcatcgcga gccgatggct gctggtgcgg ttgagtttcc gtaaaccgtg
     3601 acggtgacgc atggctcgtc cttatacttt ttccagccct gcgggcggcc agttctcgag
     3661 tttcatgccg agcgtgaggc cgcgcgaggc gagcacttcc ttgatctcgt tgagcgactt
     3721 tctgccgaga ttcggcgtct tcaggagctc ggtctcggtg cgctggatga gatcgccgat
     3781 gtagtagatg ttctcggcct tgagacagtt cgccgagcgt accgtgagct cgagatcgtc
     3841 taccggtcgg agcaggatcg gatcgatttg cgtcgccttc ttttcctcga tctgcgtcgg
     3901 catgcccttg agatcggcga agaccgaaag ctgctccacc aagacgcgcg ctgcgtagcg
     3961 caccgcctct tcgggatcga tcgcgccgtt cgtctcgata tccatgatga gcttgtcgag
     4021 atcggtgcgc tgctcgacgc gggcggactc gacggaatag ctcacgcggc gcaccgggct
     4081 gaacgaggcg tcaaggagta tcgagccgac gttgcggccc tcttcgtcaa tgctgcggcg
     4141 tgtggtcgct gcgatatagc cgcggccctg ttcgaccttg atctgcatgt cgagcttgcc
     4201 gccggaagcg aggtgcgcga tcacgtggtc gggattgacg atctcgacgt catgcgtcga
     4261 ttcgatgtcg cctgcggtca cgaccccctc gcccgatttc ttcaggttga gggtcgcctc
     4321 gtcgcggttg tgcagcttga gcacgatgcc cttcaagttg agaaggatgt cgaccacgtc
     4381 ttcctgcacg ccgtcgaggg tcgagtactc gtgcagcacg ccgctgatct tcacttcggt
     4441 cggcgcgtag cccggcatcg aggagagcag cgtacgccgc agcgcattgc cgagcgtatg
     4501 cccatagccg cgctcgaacg gctccatcgt gagcttggcg tgatacggcg agatattctg
     4561 cacatcgatg atgcggggct tcaaaaacgc actgctttgc atggcttgaa tacctcttcg
     4621 aaattacttc gagtacagct cgaccaccag cgactcgttg atcgtggagg gcagctcgga
     4681 gcgctgcggg cgcgccttga aggtgccctt gagcgccttc gtgtcgacct caacccactc
     4741 cggatagccg cgcgactcgg ccgcctcggc ggcggccttg atgcgcaact ggtccttcgc
     4801 cttctgcgcg acctcgacca catcgcccgg ccggcactgg aaggacggga tattgacccg
     4861 ttttccgttc acgaggatgc cgtcgtgacg cacgatctga cgcgcttcag tgcgcgaggc
     4921 gccgaaaccc atgcgatacg caacgctgtc gagccggctc tcgaggatct gcagcagcgc
     4981 ctccccggtc acttccttgc ggcgcgacgc ttccgcatag ccgcggcgaa actggcgctc
     5041 gagcagtccg taggtgcggc gcactttctg cttctcgcgc agctgcacgc cgtagccgga
     5101 aagccgggtg ttcttctgcc cgtgctgtcc cggcggatag ttacggcgct cgatcgcgca
     5161 cttgtcggta aagcacttct cgcctttcag gaacagcttc tcgccctcgc ggcggcactg
     5221 cctgcacctt gcgtcgagat ttctggccac agtcgatccc tctcgtcaaa tccggcgctt
     5281 cttcggcggg cggcagccgt tgtgcggcac cggcgtcacg tccgaaatgc tggtgatctt
     5341 gaaaccgcag gcgttcagcg cccgcaccgc cgactcgcgg ccgggccccg ggcctttgat
     5401 gcgcacttcg atgttcttga ccccgctctc ctgcgcgagc ctgccggcgt gctccgcggc
     5461 tacctgcgcg gcgaacggcg tggacttgcg cgagcccttg aagccgttgc tgccgcaagt
     5521 cgcccaggcg agcgcgttgc cctggcggtc agtgatggtc acgatcgtgt tgttgaagga
     5581 cgcgtgaacg tgggcgatgc cttccgccac gttcttcttg accttcttgc gcacccgggt
     5641 gcttgctttt gccatgtcgg tataccttta gacagtcgtc ggcttgatga tcttgaccgc
     5701 ggccttgcgc ggacctttac gagtgcgcgc attcgtccgc gtgcgctggc cgcgcacggg
     5761 aaggccggcg cggtgccgct tgccgcgcca cgtgccgaga tcgatcaggc gcttgatgtt
     5821 catcgagact tcgcgcctga gatcgccttc gaccgtgaac ttcgtgagct gctcgcgcag
     5881 cttgtccatt tccgagtccg acaagtcctt gaccttggtc gaaccgttca cgccggccgc
     5941 cgtgcagatc atgttcgcgc gactgcgccc gatgccgtag atcgcggtca acgcgatcga
     6001 cgcatgctga tgattcggta tgttgacgcc acctatgcgg gccatgccta cccccgaaac
     6061 ggaaaaccgg aaattttagc gcgcaagacg ctcatttaca atcgaaaatc atccctgacg
     6121 ctgcttgtgc cgcgggtcgg aacagatcac ccgcacgacg cctttgcgcc ggatgatctt
     6181 gcacttgcgg cacatccttt tcaccgatgc cagcactttc atatcgtctc ctgctttcct
     6241 ctgtacccta tttcgcgcgg aaaacgatgc gcccgcgcgt gagatcgtaa ggcgtcagtt
     6301 cgaccgttac cttgtcaccc ggaaggatgc ggatatagtg catgcgcatc ttgcccgaga
     6361 tgtgccccaa cacgatgtgg ccgttttcca gcttcacgcg gaacgttgcg ttgggcaacg
     6421 tctccacgat ctcgccctgc atctgtatgg tctcttcctt ggccatctag cgcgtaggga
     6481 agacaccgcc cttgaagttc gctttcttga gcaggctctc gtactggtgc gacattacgt
     6541 acgcctgcac ctgcgccatg aaatccatcg tcaccaccac gatgatgaga agcgacgtcc
     6601 cgccgaagta gaagggcacg tttttccaga cgatcaggaa ttcgggcagc aggcacacga
     6661 gcgtcacgta gatcgagccg gtcagcgtaa gccgcatcgt gattttctcg atgtagcgcg
     6721 cggtctggtc tccgggtcgt atgccgggaa cgaacgcacc gctctttttc aggttctccg
     6781 ccgtctcttt cggattgaag acgagcgcgg tgtagaaata gcagaagaa
//
LOCUS       DASURG010000506         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_293287, whole genome shotgun sequence.
ACCESSION   DASURG010000506 DASURG010000000
VERSION     DASURG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293287"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFY80_08960"
     CDS             complement(<1..1155)
                     /locus_tag="VFY80_08960"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEX6008079.1"
                     /translation="MSSQLSNIRPKPDKVLVDIADYVSGYSIRSKEAYSTARLCLMDT
                     LGCGFEALSYPACTKLLGPIVPGTIVPNGAKVPGTAYQLDPVTAAFNLGAMIRWLDYN
                     DTWLAAEWGHPSDNLGGILSAADWSSRNAVAAGRTPLTMRDVLTGMIKAHEIQGVIAL
                     ENSFNRVGLDHVVLVKVATTAVVTQMLGGTHEEIVNAVSNAWIDGQSLRTYRHMPNTG
                     SRKSWAAGDATSRGVRLALMALKGEMGYPSALTAKTWGFYDVLFRGKPFSLKRKYGSY
                     VMENVLFKISYPAEFHAQTAVECAIRLHRQVKDRLDDIDRIVITTHESAIRIIDKQGP
                     LYNPADRDHCIQYMVAIGLMKGALTAADYEDEAASDPRIDRLRAKMQCVED"
     gene            complement(1206..2384)
                     /gene="prpC"
                     /locus_tag="VFY80_08965"
     CDS             complement(1206..2384)
                     /gene="prpC"
                     /locus_tag="VFY80_08965"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX6008080.1"
                     /translation="MNKEESTSGFKPKKSVALSGVPAGTTALCTVGRSGNDLHYRGYD
                     ILDIADTCEFEEIAYLLVHGKLPNAAELANYKLKLRQSRGLPTAVRSVLEHVPAGAHP
                     MDVMRTGCSALGAALPEKEDHNLSGARDIADRLIASFGSMLLYWYHFSHNGRRIDVET
                     DDDSIGGHFLHLLHGKPAKSLWVKAMHTSLILYAEHEFNASTFAARVVAGTGSDMHSA
                     ITGAIGALRGPKHGGANEVGFEVMNRYETPDEAERDIVARIRNKEVVIGFGHPVYTIA
                     DPRNKIIKEVARTLSKDAGDMKMFDIAERIETVMMREKKMFANLDWFSAVSYHRMGIP
                     TPMFTPIFVIARTSGWSAHVIEQRIDNKIIRPTAVYTGPEGRHFVPLAERGQTPKRGK
                     "
BASE COUNT          431 a          791 c          843 g          536 t
ORIGIN      
        1 gtcttccacg cactgcatct ttgcgcgtag ccggtcgatg cgcggatccg aggctgcctc
       61 gtcttcgtag tccgctgcgg tgagcgcgcc tttcatgagc ccgattgcca ccatgtactg
      121 gatgcagtgg tcgcggtcgg ccgggttata gagcgggccc tgcttgtcga tgatgcggat
      181 cgcggactcg tgcgtggtga tgacgatgcg gtcaatatcg tcgagtcgat ccttgacctg
      241 ccggtgcagc ctgattgcgc attccacggc ggtctgtgcg tggaattcgg cggggtagga
      301 gatcttgaag agcacgttct ccatgacata ggacccgtac ttgcgcttga ggctgaacgg
      361 tttgccgcgg aagagcacat cgtagaagcc ccaggtcttc gcggtcagcg ccgagggata
      421 gcccatctcg cccttgagcg ccatcagcgc aaggcgcacg ccccggctcg tcgcgtcgcc
      481 cgcagcccat gacttgcgcg atccggtgtt gggcatgtgg cgataggtgc gcagcgactg
      541 gccatcgatc caagcgttcg ataccgcgtt cacgatttcc tcgtgcgtgc cccccagcat
      601 ctgcgttacg acggcggtgg tggccacctt gacgagcacc acatgatcga gcccgacgcg
      661 attgaagctg ttctcgagag cgatgacgcc ctggatctcg tgcgccttga tcatgccggt
      721 cagcacgtcg cgcatcgtga gcggggttct gcccgccgca acggcgttgc gcgacgacca
      781 gtcggcggcg ctgaggatcc cgcccaaatt gtccgaaggg tggccccatt ccgccgccag
      841 ccaggtgtcg ttgtagtcca gccagcgaat catggcgccg aggttgaacg ctgccgtaac
      901 cggatcgagc tgataggcgg tgcccggcac cttcgcgccg ttggggacga tcgtgccggg
      961 gacgatcggg ccgaggagct tcgtgcatgc gggatacgac agcgcctcga atccgcaccc
     1021 gagcgtgtcc atcaggcaaa ggcgggcggt gctgtacgct tccttgctcc tgatggaata
     1081 acccgagacg tagtcggcga tgtcaacaag gaccttgtcc ggcttaggcc ggatattcga
     1141 aagttgtgac gacattgctc gttccgttta ttggtgttct ggcatgcccg gttccggcgg
     1201 agcgtctact tgcctctctt tggcgtctgg ccgcgctccg cgagcggcac gaagtggcgg
     1261 ccctccggtc cggtgtagac cgctgtgggg cggatgatct tgttgtcaat gcgctgctcg
     1321 atcacgtgtg ccgaccagcc gctcgtgcgt gcgataacga agatcggtgt gaacatgggt
     1381 gtggggatgc ccatgcggtg ataggacacc gccgagaacc agtcgagatt cgcgaacatc
     1441 ttcttctcgc gcatcatcac ggtttcgatg cgctcggcga tgtcgaacat cttcatgtct
     1501 cccgcgtctt tcgagagcgt gcgcgcgacc tccttgatga tcttgttgcg cggatccgca
     1561 atggtgtaga ccgggtgccc gaatccgatc acgacttcct tgttgcgaat gcgggcgacg
     1621 atgtcccgct ccgcctcgtc gggggtctcg tagcggttca tcacttcgaa accgacttca
     1681 ttggcgccgc cgtgcttggg accccgcagg gcgccgatcg cgccggtgat cgcggaatgc
     1741 atatccgagc ccgtgccggc gacaacgcgc gctgcaaagg tggaggcgtt gaactcgtgc
     1801 tcggcgtaga ggatgaggga cgtgtgcatc gccttcaccc aaagcgactt ggcgggcttg
     1861 ccgtggagca gatgcaggaa gtggcccccg atcgaatcgt cgtccgtttc cacgtcgatg
     1921 cgccgcccgt tgtggctgaa gtgataccag tagagcagca tcgaaccgaa cgaggcgatg
     1981 aggcggtcgg cgatgtcgcg cgcgccggac aggttgtgat cctccttttc aggcagcgcc
     2041 gcgccgagcg ccgaacagcc ggtacgcatg acgtccatgg gatgcgctcc ggcgggcaca
     2101 tgctcgagta cgctgcgcac cgccgtgggc aggccgcgtg actgccgcag cttcaacttg
     2161 tagttcgcga gttccgcggc gttggggagt tttccgtgca ccagcaggta agcgatctcc
     2221 tcgaattcgc aggtgtcggc gatgtcgagg atgtcataac cccgatagtg gagatcgttg
     2281 ccgctacgcc ctacggtgca caacgccgtg gtgcccgccg gcacaccgga cagcgcgacc
     2341 gatttcttgg gtttgaaccc gctcgtggat tcttctttgt tcatgacatc cctcaatgtg
     2401 tcgtgtcgcc gtcctgcaaa cccggatacc gttccgaacg ggaagtcatt ccggcgcaag
     2461 ccggaatcca ggcatacccg gctggatgcc ggttttcacc ggcatgacgg aacgtcagcc
     2521 gaggagtgag cggatcgaat cgcgctcctc gtgcagctcc ttcaacgtcg cctgcatgcg
     2581 gccgcggctg aactcgctca c
//
LOCUS       DASURG010000507         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_700734, whole genome shotgun sequence.
ACCESSION   DASURG010000507 DASURG010000000
VERSION     DASURG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700734"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFY80_08970"
     CDS             complement(<1..723)
                     /locus_tag="VFY80_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX6008081.1"
                     /translation="MSTLTRTLPLLLILAGTTPTTSPAQSREGMRRAEAARYLSDVHA
                     DVKRAYYDREFRGVDLELEYDSAKARLARAQSDQERFWAIDRYLHSLKDSHTYFVGLL
                     RVGLGDFNLDFQFFGESAYVVGVDRGSYADSVGVRTGDEILEFDGQPLTRARADDVVK
                     AFLSAHPIRPLQLKVRAPDRSVAHFSIQPDTAAIYRVKGKQLRRMLSMWRDSIEYSTS
                     HAQATICGLRFLLAPAPVHARRS"
     gene            770..1267
                     /locus_tag="VFY80_08975"
     CDS             770..1267
                     /locus_tag="VFY80_08975"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX6008082.1"
                     /translation="MRRIPAQIALAILLSACELSLPTRSRAPADTAAGEIAFRLASPN
                     EAAILVPVQINGRSAIDFVLDTGATLSCIDVSLARELQLPDDDAIGGIAVGAMNVGRL
                     ETVRIDSLRIGNAVVYDVAACKLDLEVLRRGFGAHGLIGLNFLKSFDMQLDFDRNVLT
                     LTAPD"
     gene            complement(1271..2242)
                     /locus_tag="VFY80_08980"
     CDS             complement(1271..2242)
                     /locus_tag="VFY80_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6008083.1"
                     /translation="MKTILSIDGGGIRGLIPALVIAQIEQRTGRRAAELFDFFAGTST
                     GGIIALALNVPDQDGKPLYTAEALADFYRQRGEQIFDRSFMKRIVSGHGWIDELYDHR
                     PLNRILQEYFGGSLPMRAALKPVMVSTYDLHNRKPRFLKSFNPEHYALPMWLAARATS
                     AAPTYFEPADIPIESGRSVLVDGGVFVNNPAASAYAEAVRGWKNEDIFVVSIGTGSET
                     ASYDYGAAQTWGKLEWVAPLIHLVFDGVSDAVDYQMNQMLGDRFLRLQITLQYADADL
                     GNASADNLKRLEADAQRLIAAHNPEINRVCALLAARQQPPPPPMPLP"
BASE COUNT          410 a          722 c          739 g          413 t
ORIGIN      
        1 cgatcggcgt gcgtgaactg gggcaggcgc cagtaaaaaa cggagtccgc aaatggtcgc
       61 ctgcgcgtgc gacgtcgaat actcgatgct gtcgcgccac atggagagca tgcgccgcag
      121 ctgcttgccc ttcacgcggt aaatcgcggc cgtgtccggc tggatggaga aatgcgccac
      181 cgagcggtcc ggcgcccgca ctttcagctg caacggccgg atcgggtgcg ccgagaggaa
      241 ggctttcacg acgtcgtccg ctcgcgcgcg cgtgagcggc tgcccgtcga attccaggat
      301 ctcatcgccg gtgcgcacac ccacggaatc ggcgtagctg ccgcggtcga cgcccacgac
      361 gtaagcggat tcgccgaaga actggaaatc gagattgaaa tcgcccagcc cgacgcggag
      421 cagcccgacg aagtacgtgt gcgagtcctt cagcgagtgc aggtagcggt cgatggccca
      481 gaaccgttcc tggtcgctct gcgcgcgcgc caggcgggct ttggccgagt cgtattccag
      541 ctccaggtcg acgccgcgga attcacgatc atagtaggcg cgtttgacat cggcatggac
      601 atcgctgagg taacgcgcgg cttcggcgcg gcgcatccct tcacggcttt gtgcagggct
      661 ggttgtcggc gtggtgccgg ccaggatgag caggagcggc agcgttcgtg tcagcgtcga
      721 cataggcaga tgatggggat cggttcacca gcggcaaagt gagattacga tgcgacggat
      781 acctgcgcaa attgcgctag cgattctttt gagcgcctgc gagctgagtc tccccacgcg
      841 ctcgcgcgca ccggccgaca ccgccgcggg cgaaatcgcg ttccgcctgg ccagtccgaa
      901 cgaagcggcc atcctggtgc ccgtgcagat caatggccgg tccgcgatcg atttcgtgct
      961 cgacaccggt gccaccctca gctgcattga tgtttccctc gcgcgcgagc tgcagctgcc
     1021 ggatgacgac gccatcggcg gtattgccgt gggtgccatg aatgtcggac gcctggaaac
     1081 cgtacgtatc gattccctgc gcatcggcaa cgccgtcgtc tatgatgtag ctgcctgcaa
     1141 gctcgatctc gaggtgctgc gccgcggctt cggcgcgcac ggcctgatcg gcctgaactt
     1201 tctcaagtcc ttcgatatgc agctcgactt cgaccgaaac gtgctgacgc tgaccgcacc
     1261 ggattgagcg ctacggcagt ggcatgggcg ggggcggcgg ctgctgccgc gccgccagca
     1321 gcgcacagac ccggttgatt tccggattgt gcgcggcaat caagcgctgc gcatccgctt
     1381 ccaatcgctt caaattgtcc gccgacgcat taccgagatc cgcatctgcg tactgcagcg
     1441 tgatctgcag ccgcaagaaa cgatcgccca gcatctggtt catctgatag tcgacggcat
     1501 cgctcacacc atcgaatacc agatgtatga gaggcgccac ccactcgagc ttgccccatg
     1561 tttgcgccgc gccgtaatcg tagctcgcgg tttccgagcc ggtgccaatg gacacgacga
     1621 agatgtcttc gttcttccag ccgcgcaccg cttccgcata cgcggaggcc gcgggattgt
     1681 tcacgaacac gccgccatcg accagcacgg agcgccccga ttcgatgggg atatcggccg
     1741 gctcgaaata ggtgggtgcg gccgaggtcg cgcgcgcggc cagccacatg ggcaacgcgt
     1801 agtgctccgg attgaagctc ttcaggaagc gcggcttgcg gttatgcaag tcgtaggtgc
     1861 tcaccatcac cggcttgagc gcggcgcgca tcggcagtga tccgccgaag tattcctgca
     1921 gaatgcgatt gagcgggcga tgatcgtaca gctcgtcgat ccagccgtgg cccgagacga
     1981 tgcgcttcat gaaggagcga tcgaagatct gctcgcctcg ctgacgatag aaatcggcca
     2041 gcgcctcggc cgtgtagagc ggcttgccgt cctggtcggg cacgttgagc gccagcgcaa
     2101 tgatgccgcc cgtggacgtg cccgcaaaaa agtcgaacag ctcggcggca cgccgtccgg
     2161 tccgctgctc gatctgggcg atgaccaatg ccggaatcag cccgcggatg ccacccccgt
     2221 cgatgctcag gatggttttc atgactggcc tccggcaggc acgtgggcgt gcccgggcac
     2281 gcgg
//
LOCUS       DASURG010000508         9741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_598821, whole genome shotgun sequence.
ACCESSION   DASURG010000508 DASURG010000000
VERSION     DASURG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598821"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFY80_08985"
     CDS             <1..780
                     /locus_tag="VFY80_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6008084.1"
                     /translation="TLGYLAAKKDRRIASATFMTALIDFTAAGELEVFIDEAQLDALE
                     ARMQQRGYLEGAEMATTFNMLRANDLIWSFVVNNYLLGRDPFPFDLLYWNSDSTRMPA
                     AMHSYYLRNMYLKNLLREPGKLSLAGVPIDVRRIEVPAYFISTIEDHIAPWKSTYAGA
                     RLLQGPVRFVLGGSGHIAGIINPPRANKYCYWTNEARPESADEWLKGATQHAGSWWTD
                     WATWIAGRSGGKTPARILGKGALKAIEDAPGSYVRMRADTR"
     gene            818..1786
                     /locus_tag="VFY80_08990"
     CDS             818..1786
                     /locus_tag="VFY80_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008085.1"
                     /translation="MKWSAALTVLLLIFGASAAQAQTAYPSRPVRIIVPFAPGGNVDL
                     VARAIAQPLSEALRQQVIVDNRPGASGLVGTHFVAKSPPDGYTLLAMANTFAVVPSLM
                     SNPGYDPLRDFAAVSLTCRVPQVLVVNPALPVRSVKELIALAKARPGEISYASAGTGG
                     TGHMAAELFNRHAGLKMLHVPYKGNAQAIVDVISGQVTMMFDQVSTSAPHIRAGKLKA
                     LAVTSLTRSALFPDLPTVDESGVRGFEDITFNGLVAPAGTPGDVVARVHAEVAKAVAR
                     PELSKRFLERGVELAASASPQDFATAIRQEFQKKAKLAREAGIKVE"
     gene            1830..2597
                     /locus_tag="VFY80_08995"
     CDS             1830..2597
                     /locus_tag="VFY80_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6008086.1"
                     /translation="MQLRDHTFLVTGGASGLGAATARRLAEAGARVVIADLDARAGAR
                     IARECGAQASFTRTDVTREADVAAAINAAVQRCGAVHGAVSCAGIAIAERVVGRHGPH
                     RLDAFMRVMDVNVIGSFNVLRLAAQAMEKNAPDEGGERGVIINTASIAAFEGQVGQVA
                     YSASKAAIVGMTLPAARELARAGIRVMAIAPGIFETPMMATMSEEVRASLGAQVPFPQ
                     RLGRAEEFAALVAHIIENPMLNGAVVRLDGALRMAAK"
     gene            2731..5403
                     /gene="aceE"
                     /locus_tag="VFY80_09000"
     CDS             2731..5403
                     /gene="aceE"
                     /locus_tag="VFY80_09000"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX6008087.1"
                     /translation="MDMRDIRTDPDPQETREWLDALESVLEREGPDRAHFLLERLVAK
                     ARVSGAYIPFNLNTPYTNTIPLSREESFPGNAELEQKIRALVRWNAMAMVVRANKESS
                     GLGGHIASFASAATLYDVGFNHFFRAAHENFAGDLIYMQGHSAPGIYARAFLEGRITE
                     EQLAHFRREVEGKGLPSYPHPWLMPEFWQFPTVSMGLGPIMAIYQARFMRYLKDRGLV
                     EAQGRKVWCFCGDGEMDEPESLGAISLAGREQLDNLIFVINCNLQRLDGPVRGNGKII
                     QELESDFRGAGWNVIKVIWGSYWDPLFMRDKTGILLKRMEECVDGEYQAFKSHDGAFV
                     RKHFFGKYPELADMVANMSDDDIWRLNRGGHDPHKIYAAYAAAVKHQGQPTVILAKTV
                     KGYGMGEAGEGQNITHQQKKMGTASIRAFRDRFNVPIPDEKLEEVPFFRPPADSPEMV
                     YFRERIQAMGGSLPARRRKGNALEVPPLSAFEAQLKGTEGREISTTMAFVRILNTIIR
                     DKKIGKYVVPIVPDESRTFGMEGMFRQLGIYSHVGQLYTPQDADQLMFYKEDKKGQIL
                     EEGITEAGAMSSWIAAATSYSTNGVAMIPFYIFYSMFGFQRVGDLAWAAGDMRARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHVLASTVPNCIAYDPAYAYELAVIIQDGLRRMYAEQ
                     EDVYYYITVMNENYAHPQMPEGAEEGVLKGMYLLREGKAKKNQPKVQLLGSGTILREV
                     VAGAELLEREFGIAADIWSVTSFTELRRDGLSVERWNMLHPEQPPRVSYVEQCMKDRT
                     GPVVAATDYMKIFADQIRAFLPGADYIVLGTDGFGRSDTREQLRHFFEVDRYFVTVAA
                     LKALADQEVVPQAKVTEAIRKYGILPDKPEPSRT"
     gene            5434..6768
                     /gene="aceF"
                     /locus_tag="VFY80_09005"
     CDS             5434..6768
                     /gene="aceF"
                     /locus_tag="VFY80_09005"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX6008088.1"
                     /translation="MANLVEVKVPDIGDFKDVPVIEVLIAPGDSVKKEDSLVTLESDK
                     ATMEVPSPASGIVKDVRVKVGDKVSEGSPVVVLEASDGAEGGAERAAPVASHAVPTSA
                     PDVAAPGRSAPAAKPQPAAFSQPGSPPAAAPQPSVEAASVGKAHASPAVRRLAREFGV
                     DLSQVKGTGPKERIVKEDVQSYVKAVLARPRGAEGAGPGLNLPPLPQIDYAKFGPIET
                     KPLSRIRKLSGANLHRNWVSIPHITQHDEADITELETFRKAQAEEAKRSGIKFTMLGF
                     LMKASVVALKQHPEFNASLASDGEGLVIKGYYHISIAVDTPNGLVVPVIRDVDKKGLL
                     ELARELGEVSARMRAGRISPADLQGGCFSISSLGGIGGTHFTPIINAPEVAILGVGKA
                     VTKPVWNGREFAPRLMLPLSLSYDHRVIDGAQGARFISYLASVLSDIRRLVL"
     gene            6789..7448
                     /gene="nadD"
                     /locus_tag="VFY80_09010"
     CDS             6789..7448
                     /gene="nadD"
                     /locus_tag="VFY80_09010"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6008089.1"
                     /translation="MTGSAPIGILGGTFDPVHYGHLRLGQQVGETLRLDQVRFVPSGT
                     PPHRSAPRLPAPERLAMVKLAVADNALFAVDERETLRSGPAYTVDTLAELRAELGPAR
                     PLCLLVGADAFLDFATWHRWHDIFGLAHVVVAHRPGFPVETWQERMLQPLAREYAQRS
                     LGQPLAVHLAPAGGIAVVAITALDISATMIRECIRAGRSPRYLLPQPVLDYIQSRKLY
                     A"
     gene            7498..7863
                     /gene="rsfS"
                     /locus_tag="VFY80_09015"
     CDS             7498..7863
                     /gene="rsfS"
                     /locus_tag="VFY80_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6008090.1"
                     /translation="MRVEKLVSTTVAALEDVKARDIVVLDVRKMTSLFDKVIIASADS
                     TRQGKALSSRVQEKVKGIGAPIHGVEGEQNGEWILVDLGTVIVHIMQPAARQYYNLEE
                     LWTPAKRTRRRATRSTRAA"
     gene            7860..8339
                     /gene="rlmH"
                     /locus_tag="VFY80_09020"
     CDS             7860..8339
                     /gene="rlmH"
                     /locus_tag="VFY80_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX6008091.1"
                     /translation="MKFIVLSVGHRMADWVRAGFDEYARRMPRDAAIVLKEIRPAARS
                     GGSGAADVRRVLEDECRRIRAALPKGAYKVLLDERGTAMSTREFARCIARWREQGRDV
                     AFIIGGAEGTAPALKTEADLAWSLSTLTFSHGLARVMLAEQLYRAVSLLAGHPYHRS"
     gene            8461..9075
                     /locus_tag="VFY80_09025"
     CDS             8461..9075
                     /locus_tag="VFY80_09025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX6008092.1"
                     /translation="MLDKRIYLASRSPRRRELLRQIGVSFELLFLREDPRRGRDVDET
                     VLPHEAAQDYVVRLARAKAELAARVAPQRGLRPHPALGADTTVVFEGNVIGKPDGAED
                     AIRMLSALSGREHQVMTAVALALRDRIESVLSISTVEFRVLEPAEIERYVATGEPHGK
                     AGAYAIQGRAAAFITRIAGSYSGIMGLPLAETAQLLRKFDIAVI"
     gene            9093..>9741
                     /locus_tag="VFY80_09030"
     CDS             9093..>9741
                     /locus_tag="VFY80_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX6008093.1"
                     /translation="MVEEILINVTPQETRVAVIEHGVTQELHVERASARGLVGNIYVG
                     RVLRVLPGMQSAFIDIGGERAAFLHVADIRGNRHPGDHDTPIEKLLAEGQNLMVQVAK
                     DPIGTKGARLSTQISIAGRFLVYLPQESHIGISQRIEDEEERQHLREKLHQLLPPDEK
                     GGFIIRTMAETASDRELACDIEYLRKLWRGIQERALTAAALTPLYQELNLSLRVLR"
BASE COUNT         1780 a         3082 c         3188 g         1691 t
ORIGIN      
        1 acgctcggct acctcgcggc gaagaaggac cggcggatcg ccagcgccac gttcatgacg
       61 gcgctgatcg acttcaccgc ggcgggcgag ctcgaggtgt tcatcgatga agcgcagctc
      121 gatgcgctgg aggcgcgcat gcaacagcgc ggctatctgg agggggccga aatggcgacg
      181 accttcaaca tgctgcgcgc aaacgacttg atctggtcgt tcgtcgtgaa caactacctt
      241 ctcggccgcg atccgttccc gttcgatctg ctctactgga attcggactc gacgcgcatg
      301 ccggcggcga tgcacagcta ttacctgcgc aacatgtatt tgaagaacct gttgcgcgag
      361 ccggggaagc tttcgctcgc cggcgtgccg atcgacgtgc ggcgcatcga agtgcccgcc
      421 tatttcattt ccaccatcga ggaccatatt gcaccgtgga aatccactta tgccggcgcg
      481 cggctgctgc agggcccggt gcgcttcgtg ctcggcggct ccgggcacat cgccggcatc
      541 atcaatccgc cccgagcgaa caagtactgt tactggacga acgaggcgcg cccggaatcc
      601 gccgacgaat ggctgaaagg cgcaacccag cacgccggtt cctggtggac cgattgggcg
      661 acgtggatcg ccggccgcag cggcgggaag actccggcgc gcattctcgg gaaaggtgcg
      721 ctgaaggcga tcgaggatgc gccgggcagt tacgttagaa tgagggccga cacccggtaa
      781 gcgtccgcgg gcgtgggccc gcttggagga gcgcgccatg aagtggagcg ccgcactgac
      841 ggttctgctg ctgatattcg gcgcgagcgc agcgcaggcg cagacggcat atccctcgcg
      901 cccggtgcgc atcattgtgc ccttcgcgcc cggtggcaac gtcgacctcg tcgcccgtgc
      961 gattgcccaa cccctttcgg aagcgctgcg gcagcaggtg atcgtcgaca atcgcccggg
     1021 cgccagcgga ctcgtcggca cgcacttcgt ggcgaagtcg ccgcccgatg gctacaccct
     1081 tctcgccatg gcgaacacct tcgcagtggt accttcgctc atgtccaatc cgggctacga
     1141 tcccttacgg gattttgccg cggtgtcgct cacttgccgt gtgccgcagg tcctggtcgt
     1201 gaacccggcg cttcccgtgc gatcggtgaa ggagctcatt gcgctcgcca aagcacggcc
     1261 cggagaaatt tcctacgcgt ccgccggcac gggcggcacc ggacacatgg cagccgagct
     1321 tttcaatcgc catgccgggc tcaaaatgct gcacgtaccc tacaaaggca acgcgcaggc
     1381 catcgtggat gtcatcagcg ggcaggtgac gatgatgttc gaccaggtca gcacctcggc
     1441 gccccacatc cgcgccggta agctcaaggc gcttgcggtg acgagtctca cgcgctccgc
     1501 gctctttcca gacttgccca cggtggacga atccggcgtg cggggtttcg aggacatcac
     1561 gttcaacggc ttggtcgcgc ccgccggcac cccgggcgat gtcgtggcgc gcgtgcacgc
     1621 cgaagtcgcg aaagcggtgg cccggcccga gctcagcaag cgttttctcg agcgcggcgt
     1681 cgagctcgcg gcgagcgctt caccacagga cttcgccacc gcgatacgtc aggagtttca
     1741 gaagaaggcc aaactcgcgc gcgaggccgg gatcaaagtg gagtagcggc tcagcccgag
     1801 ccgcagcctg atccccattt aagtccacct tgcaacttcg cgaccacacc tttctcgtca
     1861 cgggcggcgc ttccggactg ggcgccgcga cggcgcggcg gctcgcggaa gccggtgccc
     1921 gggtcgtcat cgcggatctc gatgcgcgag ccggcgcgcg cattgcacga gaatgcggtg
     1981 cgcaagcgag tttcacccgc acggatgtca cccgggaggc cgacgtggct gccgcgatca
     2041 acgccgctgt ccagcgctgc ggtgccgtgc acggtgcggt gagctgcgcc ggcatcgcga
     2101 ttgcggagcg ggtcgttggc cgccacggcc cgcatcggct cgatgcgttc atgcgtgtga
     2161 tggacgtcaa cgtgatcggc agtttcaacg tgctgcgtct cgctgcgcag gcgatggaga
     2221 agaatgcgcc agacgaaggc ggtgaacgcg gcgtcatcat caataccgca tcgatcgccg
     2281 cgttcgaagg gcaggtgggg caggtcgcgt acagcgcgtc gaaagcggca attgtcggca
     2341 tgacgctccc cgcggcgagg gagctcgcgc gcgccggcat acgcgtcatg gcgattgcac
     2401 cgggaatctt cgaaacgccg atgatggcga ccatgagcga agaagtgcgc gcatcgctcg
     2461 gtgcgcaggt tccttttccg caacggctgg gtcgcgcgga agagtttgcg gcactcgtgg
     2521 cgcacatcat cgaaaacccg atgctgaatg gcgcggtggt gcgcctcgac ggggcgctcc
     2581 gcatggcggc aaagtgacgc tctgccgagc atttcactgc gctgcagcat gcggcgcctt
     2641 ctcaagtgcg gctataatcg attcgcccgc tgcacagtct taagggcgtc ccgtttttct
     2701 ttctcaaatg gcccaattta ggcagacgcc atggacatga gagacattcg caccgatccc
     2761 gatccgcagg aaacccggga gtggctggat gcgctggaat ccgtgctcga gcgcgaaggg
     2821 ccggatcgcg cccatttcct gctcgagagg ctggtggcga aggcccgggt gtccggcgcc
     2881 tacattccgt tcaatctcaa tacgccctac accaacacga ttccgctgtc gcgcgaagaa
     2941 agttttcccg gcaacgccga gctcgagcag aaaattcgag cgctcgtgcg ctggaacgcg
     3001 atggcgatgg tcgtgcgggc gaacaaggag tcttccggtc tgggtgggca catcgcgagc
     3061 tttgcctccg ccgcgacgct ctacgatgtc ggattcaacc atttctttcg cgcggcgcat
     3121 gaaaacttcg cgggcgacct gatttacatg caggggcact cggcgccggg catctatgca
     3181 cgcgcgtttc tcgagggccg catcaccgaa gagcaactcg cgcatttccg ccgtgaggtg
     3241 gaaggcaagg gtcttccgtc ctatccccac ccctggctca tgcccgagtt ctggcagttt
     3301 cccacggtgt cgatgggtct cggaccgatc atggcgatct accaggcgcg cttcatgcgc
     3361 tacctcaagg atcgcgggct ggtcgaagcg caggggcgta aggtgtggtg tttctgcggc
     3421 gatggcgaga tggacgagcc cgaatcgctc ggcgccattt ctctggccgg ccgcgagcag
     3481 ctcgacaacc tcattttcgt catcaactgc aacctgcagc gcctcgatgg cccggttcgc
     3541 ggcaacggca agatcatcca ggaactcgag agcgatttcc ggggtgccgg gtggaacgtg
     3601 atcaaggtga tctggggctc gtactgggac ccgctcttca tgcgcgacaa gaccggcata
     3661 ctgctgaagc gcatggagga gtgcgtggac ggcgagtacc aggcattcaa gtcgcacgac
     3721 ggcgccttcg tgcgcaagca cttctttggc aagtatccgg agcttgccga catggtcgcc
     3781 aatatgtcgg acgacgacat ctggagactc aaccgcggcg gacatgatcc gcacaagatc
     3841 tatgcggcct atgctgcggc cgtgaagcac cagggccagc ccacggtcat tctggcgaag
     3901 acggtgaagg gctacggcat gggcgaggcg ggcgaaggtc agaatatcac gcaccagcag
     3961 aagaagatgg gcacggcttc tatccgcgcg ttccgcgacc gcttcaacgt gccgatcccc
     4021 gacgaaaagc tcgaggaggt tccgttcttc aggccgccgg cggacagccc cgaaatggtg
     4081 tacttccggg agcgcatcca ggcgatgggc gggagcctgc cggcgcgacg ccgcaagggc
     4141 aatgcactcg aagtgccgcc gctttcggct ttcgaagcac agttgaaggg caccgagggc
     4201 cgcgagattt cgaccacgat ggcgttcgtg cggatcctga ataccatcat acgcgacaag
     4261 aaaatcggaa agtacgtcgt gcccatcgtg ccggacgagt cgcgcacttt cggcatggaa
     4321 ggcatgttcc gccagctcgg catctattcg cacgtcggcc agctctacac gccgcaggac
     4381 gccgatcagc tcatgttcta caaggaggac aagaagggcc agatcctgga ggagggcatc
     4441 accgaagcgg gcgcgatgtc ctcgtggatc gcggcggcaa cttcgtatag cacgaacggc
     4501 gtggcgatga ttccgttcta catcttctat tccatgttcg gattccagcg ggtaggcgac
     4561 ctcgcgtggg cggcgggcga catgcgcgcg cgcggcttcc ttctgggcgg gacggccggg
     4621 cgcactacgc tgaacggcga ggggctgcag cacgaggacg ggcacagcca cgtgctcgcc
     4681 tccaccgtgc cgaactgcat cgcctacgat cccgcttacg cgtatgaact cgcggtgatc
     4741 attcaggacg gtcttcgccg catgtatgcg gagcaggaag acgtctacta ctacatcacc
     4801 gtcatgaacg agaactacgc tcacccgcag atgcccgagg gcgcggaaga gggcgttctg
     4861 aaaggcatgt atctcctgcg tgaaggcaag gcgaagaaga accagcccaa ggtacagctt
     4921 ttgggcagcg gcacgatctt gcgcgaagtg gtcgccggcg cggagctcct cgaacgggag
     4981 ttcggcatcg cggccgacat ctggagcgtc acgagtttca cggagctgcg gcgcgacggg
     5041 ctgtcggtgg agcgctggaa catgctgcat cccgagcagc cgccgcgagt gagttacgtc
     5101 gagcagtgca tgaaggacag aacgggcccc gttgtcgcgg ccaccgatta catgaagatc
     5161 tttgcggacc agattcgtgc cttcctgccg ggggcggatt acatcgtgct cggcacagac
     5221 ggcttcgggc gctccgacac gcgcgagcag ctgcgtcact tcttcgaggt cgaccgctac
     5281 ttcgtcaccg tggcggcgct caaagcgctc gccgatcagg aggtcgtgcc gcaggcgaaa
     5341 gtcaccgaag cgatccgcaa gtacggcatc cttcccgaca agcccgagcc cagccggacc
     5401 tgacgtcagt tcagacaaga agaacatttc gcaatggcaa acctggtcga agtgaaggtg
     5461 ccggacatcg gtgatttcaa ggatgtgccg gtgatcgagg tcctgatcgc acccggcgac
     5521 agcgtgaaga aggaggactc gctcgtcacg ctcgagtccg acaaggcgac catggaggtg
     5581 ccttcgccgg cgagcgggat cgtgaaggac gtgcgcgtca aggtcggcga caaggtctcg
     5641 gaagggtctc cggtcgtcgt gctggaagcg tccgatggcg cggaaggagg tgccgagcgc
     5701 gccgcacccg tcgcgagtca cgccgtgccg acctctgcac ccgatgtcgc tgctcccggc
     5761 cgttcggcgc cggccgcgaa accgcagcct gcggcttttt cccagcccgg ctcgccgccg
     5821 gctgccgcgc ctcagccgtc cgtcgaagcg gcaagtgtcg gaaaggctca cgcgagccct
     5881 gcggtgcgcc ggctcgcgcg cgagttcggc gtcgatctgt cgcaggtcaa gggcacaggg
     5941 ccgaaggagc gcatcgtcaa ggaggacgtg cagagctacg tcaaggctgt gcttgcgcgc
     6001 ccgcgcggag ccgaaggtgc aggtcctggg ctcaatctgc cgcccttgcc gcagatcgat
     6061 tatgcgaaat tcgggccgat cgagacgaaa cctctttcgc gcatccgtaa actctcgggc
     6121 gcgaatctgc accgcaattg ggtaagcatt ccgcacatca cgcagcacga cgaggcggac
     6181 atcaccgagc tcgagacctt tcgcaaggcg caagccgagg aagcgaaacg aagcggcatc
     6241 aagttcacga tgctgggctt tctcatgaag gcatccgtcg tggcgctcaa gcagcatccc
     6301 gagttcaatg cgtcgctcgc gtccgacggc gaaggtctcg tgatcaaggg ctactaccac
     6361 atcagcatcg cggtcgacac gcctaacggg ctcgtcgtgc cggtgatacg cgacgtagac
     6421 aaaaaaggcc tgctcgagct tgcccgcgag ctgggcgagg tcagcgcgcg catgcgcgcc
     6481 ggaaggattt ctcccgccga tctacagggc ggctgtttct cgatttcgag tcttggcggc
     6541 attggtggca cgcatttcac gcccatcatc aatgccccgg aagtcgcgat tctcggagtg
     6601 ggcaaggcgg tgacgaaacc ggtgtggaac gggcgcgaat tcgcgccgcg gctgatgttg
     6661 ccgctttccc tgtcgtacga ccatcgggtg atcgacggcg cacagggcgc gcgcttcatt
     6721 tcttatctgg cgagcgtgct gtcggacatc cgccgactcg tgctgtagcc tgcgcagcct
     6781 cgagtctcgt gaccggctct gcgcccatcg gcatcctcgg cggcacattc gatcccgtcc
     6841 actacggcca tctccgactc ggccagcagg tcggcgagac gctcaggctc gatcaagtgc
     6901 ggtttgtccc gagcgggacg ccaccgcatc gcagcgcgcc gcgcctgccg gcacccgagc
     6961 ggctcgcgat ggtcaaactc gccgtcgcgg acaacgcgct cttcgcggtg gacgaacgcg
     7021 agacgctgcg cagcggtccg gcctacaccg tggacacgct tgcggaactg agggcggaac
     7081 tgggtccggc gcggccgctg tgcctgctgg tgggcgcgga cgcgtttctc gactttgcga
     7141 cgtggcatcg ctggcacgat atcttcggtc tcgcgcacgt cgtggtggcg caccggccgg
     7201 gcttcccggt ggagacatgg caggagcgca tgctacagcc gctcgcgcgg gaatacgcac
     7261 aacgctcgct cgggcagccg ctcgccgtgc atctggcgcc tgcgggcggt atcgcggtgg
     7321 tcgcgatcac cgcgctcgac atttcggcga cgatgatacg cgagtgcata cgcgccggac
     7381 gaagcccgcg ctatttgctt ccgcaacccg tgctcgatta tattcagtcg cgcaagctct
     7441 acgcctgatg caccgtcacc aattcaatgg ggcacgcgac ttttcgacag gaggtcgatg
     7501 agagtagaga agctcgtgag taccacggtt gcagcgctgg aagacgtaaa agcgcgcgat
     7561 atcgtggtgc tcgacgtcag aaaaatgacg tcactgttcg acaaagtcat catcgccagc
     7621 gccgattcca cgcgccaggg taaagcgctt tcgagccgcg tgcaggaaaa agtgaagggc
     7681 atcggcgctc ccatccacgg cgtggaaggc gagcagaacg gcgagtggat actcgtcgat
     7741 ctcggcaccg tgatcgtaca catcatgcag ccggctgcgc ggcagtatta caacctcgag
     7801 gaactctgga cgccggcgaa gcgcacgcga cgccgcgcta cccgcagcac gcgggcggcg
     7861 tgaaattcat cgtcctctcc gtagggcaca gaatggcgga ctgggtccgc gccgggttcg
     7921 acgaatatgc gcggcgcatg ccgcgcgatg cggcgatcgt cctgaaggag atcaggcccg
     7981 ctgcgcgtag cggcgggtcc ggcgccgctg acgtacggcg ggtgctggaa gacgaatgtc
     8041 gccgaatccg ggctgcgctt ccgaaaggcg cttacaaagt gctgctcgac gaacgcggca
     8101 ctgccatgtc cacccgggag ttcgcgcggt gcatcgcgcg ctggcgcgag caggggcgcg
     8161 atgtcgcctt cattatcggg ggcgccgagg gcactgcgcc ggccctgaag accgaggcgg
     8221 atctcgcctg gtcgctctcc acgctgacgt tttcccacgg actcgcgcgc gtgatgctcg
     8281 cggagcagct ctaccgtgcc gtctcgctcc tggccgggca cccgtatcac cgctcctgag
     8341 cacgcggttt cgcgcagggc aaaagtcgct tcatcacttc aggcactcta aggtagcatt
     8401 gggcaggttg cgagcgcggc cgccacggcc gttgccgcct tcctcggaca gaacaacatc
     8461 atgctggaca agcgcatcta cctcgcttcg cggagcccgc ggcgccgcga gctgttacgc
     8521 cagatcggcg tgagcttcga gctgctattc ctgagagagg acccgcgccg ggggcgcgac
     8581 gtggacgaga ccgtgctgcc gcacgaggcg gctcaagact acgtggttcg tctcgcacgt
     8641 gcgaaagcgg agcttgctgc gcgggtcgcg ccgcaacgag gcttgcgccc gcatcccgcg
     8701 ctcggagcgg acaccacggt ggttttcgag ggtaacgtga tcggcaagcc cgacggggcc
     8761 gaggatgcca ttcgcatgct ctccgcgctt tcgggtcgtg agcaccaggt catgacggcc
     8821 gtcgcgcttg cgctgcgcga tcgtatcgaa agtgtgctct ccatatccac cgtggagttt
     8881 cgcgtgctcg aacccgctga aattgagcgc tatgtcgcaa ccggagagcc ccacggcaag
     8941 gccggagctt atgccataca gggccgtgca gccgccttca tcacccggat cgccggaagc
     9001 tattccggca tcatgggcct gccgctcgcc gagaccgccc agctgctgcg caagttcgat
     9061 atcgcagtca tctgaagctt gcgcttcccg cgatggtcga ggaaatcctg atcaacgtca
     9121 cgccgcagga gacgcgggtc gccgtgatcg agcacggcgt cacgcaggag ctgcacgtcg
     9181 agcgcgcgag tgcgcgcggc ctggtcggca acatctacgt gggccgcgtg ctgcgcgtgc
     9241 ttcccggcat gcagtcagcc ttcatcgaca ttggcggcga gcgcgccgca tttctccacg
     9301 tcgccgatat ccgcggcaat cgccaccccg gagaccatga cacgccgatc gagaagctgc
     9361 tcgcggaggg ccagaacttg atggtgcagg ttgcgaagga cccgatcggc accaagggtg
     9421 cgcggctttc cacgcaaatc agcattgccg gccggtttct cgtctacctg ccccaggagt
     9481 cccacatcgg catttcgcaa cgcatcgaag acgaggagga acgccagcac ctccgggaaa
     9541 aactgcatca gctgctgccg ccggacgaga aaggcggctt catcattcgc accatggcgg
     9601 agaccgcatc ggatcgcgaa cttgcgtgcg atatcgagta cttgaggaag ctctggcgcg
     9661 gcattcagga gcgtgcgcta acggcggccg ccctgacgcc gttgtatcag gagctcaacc
     9721 tcagcctgcg cgtgctgcgc g
//
LOCUS       DASURG010000509         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1108797, whole genome shotgun sequence.
ACCESSION   DASURG010000509 DASURG010000000
VERSION     DASURG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108797"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFY80_09035"
     CDS             <1..530
                     /locus_tag="VFY80_09035"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX6008094.1"
                     /translation="LVREDPRRGRDVTESVVDGEGAAAYAGRIARAKAEVAARQALQR
                     GLRPYPTLGADTSVVLDGAILGKPDNAQHAAQILRALSGREHEVMTAVAIALRDRVET
                     ALSISTVEFRELSDDEIYGYVRFGEPYGKAGAYAIQGRAAIFVRRIAGSYSGIMGLPL
                     AETAELLSRFDIEVV"
     gene            549..2003
                     /gene="rng"
                     /locus_tag="VFY80_09040"
     CDS             549..2003
                     /gene="rng"
                     /locus_tag="VFY80_09040"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEX6008095.1"
                     /translation="MIEEILINVTPQESRVAVIEHGVTQELHFERTSARGLVGNIYMG
                     RVLRVLPGMQSAFIDIGGERAAFLHVADIWGNRHAGEHDTPIEKLLAEGQNLMVQVAK
                     DPIGTKGARLSTQISIAGRFLVYLPQDSHIGISQRIEDEEERQHLRVKLQQLLPPDEK
                     GGFIIRTMAETAADRELASDIEYLRKLWRGIQERAQTADPLTLLYQDLNLSLRVLRDF
                     VHEETARIMVDSHETFQSMQTFAQEFTPNVVERIEHYQGERPLFDLYGVEDEIEKALA
                     RRVELKSGGYLIIDQTEALTTIDVNTGGYVGVRSFDDTIFKTNLEAAQVIARQLRLRN
                     LGGIIIIDFIDMDSEEHRNAVLNEFRKALARDRTRMTVNGFTQLGLVEMTRKRTRESL
                     AHVLCEPCPTCQGRGELKTAQTVCYQILRELLRESRQFNAREFRILASQQVIDQFLDE
                     ESQSLAMLSDFIGKPVSLLVENVYNQEQFDIILV"
     gene            2041..>2630
                     /locus_tag="VFY80_09045"
     CDS             2041..>2630
                     /locus_tag="VFY80_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6008096.1"
                     /translation="MQLREGAGHTPTSAPFVRAIAEQAFSRAAGAPLVPGNQVRLLKD
                     STENYPAWREAIQSARHKLYFENYIVADDRVGREFVAALADRARAGVRVRAIYDWIGA
                     RGAASPRLWRPLIEAGGEVRCFNPPRLESPFGWLARDHRKMIAVDGRVGFVTGLCVSE
                     KWNGSHTPGGEPWRDTGVEVQGPAVADIERAFAEVWS"
BASE COUNT          487 a          847 c          869 g          427 t
ORIGIN      
        1 tgctggtgag ggaagatccg cgccgcgggc gcgatgtcac cgaatcggtg gtcgatggcg
       61 agggcgccgc cgcctatgcc gggcgcatcg cgcgcgccaa ggccgaagtc gccgcacggc
      121 aggcgctgca aagagggctg cggccgtacc cgacgctcgg cgccgacacc agcgtcgtgc
      181 ttgacggtgc gattctgggc aagccggaca acgcgcagca cgcggcgcag atactgcgtg
      241 cgctttcggg gcgcgagcac gaagtcatga cagcggtcgc catcgccttg cgcgatcgcg
      301 tcgaaaccgc actttcgatc tcgaccgtcg agttccggga actctccgac gacgaaatat
      361 acggttacgt tcgtttcggc gagccttatg gcaaggccgg cgcctacgcg attcagggcc
      421 gggcggcgat atttgtccgg cggatcgccg gcagctattc cggtatcatg gggctgccgc
      481 ttgccgaaac ggcggaattg ctgagccgct tcgacatcga agtcgtctga gcgccgtgcg
      541 ctttcgcgat gatcgaggaa atcctcatca atgtgacgcc gcaggaatcg cgggtcgccg
      601 tcatcgagca cggcgtcacg caggagctgc atttcgagcg gaccagcgcg cgcggactgg
      661 tcggcaacat ctacatgggc agggtattgc gcgttctgcc gggaatgcaa tccgcattca
      721 tcgacatcgg cggtgagcgc gcggcctttc tgcacgtcgc cgacatctgg ggcaaccggc
      781 acgccggcga acacgatacc ccgatcgaaa agctcctcgc cgaaggtcag aacctcatgg
      841 tgcaggtggc gaaggatccc atcggcacca agggtgcgcg gctctcgacc cagatcagca
      901 ttgccgggcg cttcctggtt tatctcccgc aggactcgca catcggcatt tcgcagcgca
      961 tcgaggacga ggaggagcgc cagcacctgc gggtgaagct gcagcagctc ctgccgccgg
     1021 acgagaaggg cggcttcatt atccgcacca tggccgagac ggccgccgac cgggagctcg
     1081 cctccgacat cgagtacctg cgcaagctct ggcgcggcat ccaggagcgg gcgcagaccg
     1141 ccgaccccct gaccctgctc tatcaggacc tcaacttaag cctgcgcgtg ctgcgcgact
     1201 tcgtgcacga agagaccgcg cgcatcatgg tcgactccca cgaaaccttt cagagcatgc
     1261 agacctttgc gcaggaattc acgccgaacg tcgtggagcg catcgagcat taccaggggg
     1321 agcgtccgct cttcgacctc tacggcgtcg aggacgagat cgagaaagcg ctggcgcggc
     1381 gcgtcgaact caaatccggc ggatacctga tcatcgacca gaccgaggcg cttaccacca
     1441 tcgacgtcaa taccggcgga tacgtgggcg tgcgcagttt cgatgacacc atattcaaga
     1501 ccaatctcga ggcggcgcag gtcatcgcgc ggcagctgcg cctccggaac ctgggcggca
     1561 tcatcatcat cgatttcatc gacatggaca gcgaggagca ccgcaacgcc gtgctgaacg
     1621 aattccgcaa ggcgcttgcc cgcgaccgca cccgcatgac ggtgaacggg ttcacgcagc
     1681 tgggactggt cgagatgacg cgcaagcgca cccgcgagag tctcgcgcac gtgctgtgcg
     1741 agccctgtcc cacatgccag ggccgcgggg aactcaagac cgcccagacc gtctgttacc
     1801 agatcctgcg cgaacttctg cgcgaatcgc gacagttcaa cgcgcgcgag ttccgcatcc
     1861 tcgcgtccca gcaggtgatc gaccagttcc tcgacgagga atcgcagagt ctcgcgatgc
     1921 tgtctgactt catcgggaag cccgtgtcgc tgctggtcga aaacgtgtac aaccaggaac
     1981 agttcgacat catcctcgtg tagcgcgccc gtcgcgcggg cgagcgatac actgcccggc
     2041 atgcaactac gtgaaggagc agggcacacg cccacctccg cccccttcgt gcgcgcgatc
     2101 gccgagcagg cgttctcgcg cgcggccggc gcaccgctcg ttccggggaa ccaggtacgg
     2161 ctcctgaagg attcgacgga aaattatccg gcgtggcgcg aagcgattca gtcagcccgg
     2221 cacaagctct atttcgagaa ctacatcgtc gcggatgacc gcgtcggccg tgagttcgtg
     2281 gcggcgctcg cggacagggc gcgcgccggg gtgcgggtgc gggcgatcta tgactggatt
     2341 ggcgcgcgag gagccgcgtc gccccggctg tggcggccgc tgatcgaggc ggggggcgag
     2401 gtgcggtgct tcaatccacc gcggctcgag agcccgttcg gctggctcgc gcgcgatcat
     2461 cgcaagatga tcgcggtgga cgggcgcgtc ggcttcgtca ccggtctctg cgtcagcgaa
     2521 aagtggaatg gcagccacac gccgggaggc gagccttggc gtgataccgg cgtcgaagtc
     2581 caggggccgg cggtcgccga catcgaacgg gcgttcgccg aagtatggtc
//
LOCUS       DASURG010000510         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_394436, whole genome shotgun sequence.
ACCESSION   DASURG010000510 DASURG010000000
VERSION     DASURG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394436"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFY80_09050"
     CDS             <1..1109
                     /locus_tag="VFY80_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6008097.1"
                     /translation="GASDAGLIPMVYPDYQSVENPAVRAHFEEFWGTALDPKRGLTVV
                     EIMHAIKDGVIKGMYIMGENPAMSDPDVQHAREALAALEHLVVQDIFLTETAWHADVV
                     LPASAHAEKWGTFTNTNRQVQIARPVLDPPGGARQDWMLIQEIARRIGLDWSYRDVPD
                     VFAEMAQVMPSLRNITWERLLAEDAVTYPCDAPDQPGNEIIFANAFPTASGRAKIVPA
                     DLLPPDELPDETYPLVLTTGRLLEHWHTGAMTRRASNLDLLEPEAIAALHPRELARMG
                     IAPGEVIRVSTRRGEVVLKARADRDVAEGMVFIPFCFAEAAANLLTNPQLDPFGKIPE
                     FKFCAARVERAAPAAEAAEYRGAGLREAFTRRPA"
     gene            complement(1090..1389)
                     /locus_tag="VFY80_09055"
     CDS             complement(1090..1389)
                     /locus_tag="VFY80_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008098.1"
                     /translation="MRSSVLCVLMCLGLDAVPSGALAHDWYPIECCTGRDCTRVDRVD
                     RMPDGTMRMFVGAIEVVVPKGFTQRPSMDNDAHVCLRRKSDGTYAPRCVFMPAGV"
     gene            complement(1543..2031)
                     /locus_tag="VFY80_09060"
     CDS             complement(1543..2031)
                     /locus_tag="VFY80_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6008099.1"
                     /translation="MTARYEFAIKGRRGLPKAYHFKGSGLPNVYLLNGVKVEQDPDYG
                     ELVTIDHLPDLFMAIAFMLVRKPERLTGPEMRFLRKRMKMTQAELAKELWVSDQTVAN
                     YEKGKTEDGPADRALRLLFLAHVIDDGDLADELRRAAEELMLPSHKGRTETPGPWLFA
                     TQ"
BASE COUNT          378 a          730 c          683 g          359 t
ORIGIN      
        1 agggcgcgtc ggacgcgggc ctcatcccga tggtgtaccc ggactaccag tcggtcgaga
       61 acccggcggt gcgcgcccac ttcgaggagt tctgggggac ggcgcttgat cccaagcgcg
      121 gcctgacggt ggtcgagatc atgcacgcca tcaaggatgg cgtgatcaag ggcatgtaca
      181 tcatgggtga gaacccggcc atgtccgacc ccgacgtgca gcacgcgcgc gaggcgctcg
      241 ccgcgctcga gcatctcgtc gtgcaggaca ttttcctcac cgagacggcc tggcacgccg
      301 acgtcgtgct gccggcctcc gcccacgccg agaagtgggg aacgttcacc aataccaacc
      361 gccaggtcca gatcgcgcgc ccggtgctcg atccgcccgg cggggcgcgc caggactgga
      421 tgctgatcca ggagatcgca aggcgcatcg ggctcgactg gagctaccgc gacgtgccgg
      481 atgtcttcgc cgaaatggcg caggtgatgc cgtctctcag gaacatcacc tgggagcggc
      541 tgctggccga ggacgcggtg acctatccct gcgatgcgcc ggaccagccc ggcaacgaga
      601 tcatcttcgc caacgcgttc ccgaccgcct cggggcgcgc caagatcgtt ccggccgatc
      661 ttctgccgcc ggacgagctt cccgacgaga cctatcctct ggtgctcacc acggggcgcc
      721 tcctcgagca ctggcacacg ggagccatga cccgccgcgc cagcaacctc gacctgctcg
      781 agcccgaggc catcgcggcc ttgcacccca gggagcttgc gcggatgggc atcgctcccg
      841 gcgaggtcat tcgcgtgtcg acgcgcagag gcgaggtcgt tctcaaggcc cgcgcggatc
      901 gcgatgtggc cgaaggtatg gtgttcatcc ccttctgctt cgccgaggcg gccgccaatc
      961 tgctcaccaa cccgcagctc gaccccttcg gcaagattcc ggagttcaag ttctgcgcgg
     1021 ctcgcgtcga gcgcgcggca ccggcggcgg aagcagcaga gtatcgcggg gccggcctgc
     1081 gcgaggcgtt cacacgccgg ccggcatgaa aacgcagcgt ggtgcgtatg tcccgtcgct
     1141 cttgcggcgc aggcacacgt gcgcgtcgtt gtccatggag ggacgctgcg taaatccctt
     1201 gggcacgacc acctcgatcg ccccgacgaa catccgcatg gtcccgtccg gcatccggtc
     1261 gacgcggtcg acccgcgtgc agtcccgtcc ggtgcagcat tctatgggat accagtcgtg
     1321 agcgagggcg ccgctcggga cggcatcgag ccccaggcac atcagcacgc agagcacact
     1381 cgagcgcatt cacctttccc gcggcccaat accgtccgga caacaaacag tggacagccg
     1441 cgctgagttc cgtgagctcg tgtgccatgg cgaacggcaa aggccatcgg taatcgacca
     1501 ggaggccgcg atcggcacgc ctctgcccag caccgagcag tgctactgcg tcgcaaaaag
     1561 ccaggggcca ggcgtctcgg tccggccctt gtgcgagggt agcatcagct cctccgcagc
     1621 ccgccggagc tcgtccgcca ggtcgccgtc atcgatcacg tgggcgagga agagaagccg
     1681 aagcgcgcga tcggcaggac catcctcggt cttgcccttc tcatagttgg caacggtctg
     1741 atcactgacc cacagctcct tggccagctc ggcctgtgtc atcttcatcc gcttgcgcag
     1801 gaagcgcatc tcgggtccag tgaggcgctc gggcttgcga acgagcatga atgcgattgc
     1861 catgaagaga tcgggaaggt gatcgatcgt gaccaactcg ccatagtcgg ggtcctgctc
     1921 gaccttcacc ccattcagga gatagacgtt tggcaagccc gagcccttga agtggtacgc
     1981 cttgggcagg ccgcggcggc ccttgattgc aaactcgtaa cgtgcggtca tgggtcgatc
     2041 cacttgacag ttacgaagat cagtctctcc ccgcgtacaa ccgtgacgac gcctgcctca
     2101 cgtgacgtcc aggggagctt gtctactact cgacacttcc attcgtccgg
//
LOCUS       DASURG010000511         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_700942, whole genome shotgun sequence.
ACCESSION   DASURG010000511 DASURG010000000
VERSION     DASURG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700942"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFY80_09065"
     CDS             complement(<1..493)
                     /locus_tag="VFY80_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6008100.1"
                     /translation="MGKGVDPIKAEVIARFLLATAEEMGATLTRTAFSPNIKERADCS
                     TAIFDAKGEVIALAQRVPIHLGSMVGAVDEMLKRFALKDIVAGDMFIANDPYNGGGTH
                     LPDITVIAPVFIGKRIAAYVANIAHHADVGGMVPGSEAAVCKSIYQEGVRIPPVRIMR
                     AGKV"
     gene            complement(478..2562)
                     /locus_tag="VFY80_09070"
     CDS             complement(478..2562)
                     /locus_tag="VFY80_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6008101.1"
                     /translation="MKAVCVGIDTGGTFTDLVAADLDRGRYHYHKVPTTTRDPARGIL
                     EGVAEILDRNDLARADVTFLVLGTTLATNAVLEGKWARSGVITTRGFRDVLELARQRR
                     PHYFNLDVPKPMPPAARDCRIEVNERIAHDGTVVAPLDEDGLKSAVDVLKGKKVEAVA
                     ICFMHAYANPDHEARAKALVQALWPEVYLCTSSEVLAEFREFERFATAAVNASLMPIM
                     DRYLERFERGVADLGIAATPRVMQSNGGAVSPGAVRRAPVNTFFSGPAGGVIGSVALG
                     KALGLSHLITFDMGGTSTDVCLIRDGEPAKKSERQMAGFPVRTRTIDIHTIGAGGGSI
                     AWVDAGGLLKVGPQSAGAEPGPAAYGRGGARATVTDANVVLGRLNPRALLGGRMAVYP
                     DRARAVIDELARTVGVDPIQAAAGVLEIINVNMMGAVRVISVEQGEDPRDFALVAFGG
                     AGPLHAADVARNMGMRKVVVPPRPGLLSAIGLIHADLRGDFSLTRLLRAEAQNVAALN
                     RGLAELKKRGTAWIKAEGEREARFQWYADLRYFGQNFELILELKAERLEAATLARLVE
                     SFHHRHKDHYGYDMRGQPVEIVNLRLVVTARRRAPPAGRTIARRGDIRQALLESRKAW
                     FATSGFTATPVYDRERLPAGCRIAGPAIIEQMDTTTVVPPQAVVKSDSLGYLHMELAP
                     LAQKRGRQWEKA"
     gene            complement(2734..2964)
                     /locus_tag="VFY80_09075"
     CDS             complement(2734..2964)
                     /locus_tag="VFY80_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008102.1"
                     /translation="MSKLLSTLIAVAFAAASATAAAQATPATPAQPATPAAQDQPKAD
                     KPKASKAKKTKKSTTKKTEGKSKAKGKKTDTQ"
     gene            complement(3046..>3801)
                     /locus_tag="VFY80_09080"
     CDS             complement(3046..>3801)
                     /locus_tag="VFY80_09080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008103.1"
                     /translation="GNIGAEMAAKSPPDGYTIVFAYSGTHAINPSIYSRMPFKESDFA
                     PIIWLSSVPQVLVVHPSLPVKNVKELVALAKKRPGQLTYGSSGNGAFNHLTGALFANM
                     AKIDIVHVPYKGGGPAAVALLSGEITMILGEPASIVGFVKSGKVRALAVTGEKRALGM
                     PDLPTMAEAGVAGYSATSWNGMLAPAGVPAPIIKRLNDAFNRIIGEPDMRKRMLDNGY
                     EPVGGTPEQFGTHIRAEIAKWAPVVKATGVRVD"
BASE COUNT          601 a         1280 c         1210 g          710 t
ORIGIN      
        1 tcaccttccc cgcgcgcatg atgcggaccg gcggaatgcg caccccttcc tgatagatcg
       61 atttgcacac cgccgcttcc gagcccggta ccatgccgcc cacgtccgcg tgatgcgcga
      121 tattggcaac gtaggcggcg atgcgctttc cgatgaagac cggcgcgatg acggtgatgt
      181 ccggcagatg cgtgccgccg ccgttgtagg gatcgttcgc gatgaacatg tcgcccgcga
      241 cgatgtcctt cagcgcaaac cgcttcagca tctcgtccac cgcgcccacc atcgagccga
      301 gatggatcgg cacgcgctgt gcaagcgcga tcacttcccc tttcgcatcg aagatcgcgg
      361 tcgagcagtc ggcgcgctcc ttgatgttgg gcgagaacgc ggtgcgcgtg agcgtcgcgc
      421 ccatttcctc cgcggtggcg agcaggaagc gggcgatcac ttcggccttg atcggatcta
      481 cgccttttcc cattgccgcc cccgcttctg cgccagcggc gcaagctcca tgtgcagata
      541 acccaggctg tcgctcttga ccaccgcttg gggcggcacg acggtcgtcg tatccatctg
      601 ctcgatgatc gccggcccgg cgatgcggca tcctgcgggc agccgctcgc ggtcatatac
      661 cggcgtcgcc gtaaagcccg acgtcgcaaa ccaggctttg cggctttcga gcagcgcctg
      721 cctgatgtca ccgcgcctcg cgatggtgcg cccggcaggc ggcgcacggc gccttgcggt
      781 caccacgagc ctcaggttga cgatttccac cggctgcccg cgcatgtcat aaccgtagtg
      841 gtccttgtgg cggtggtgga aggactcgac gagccgcgcc agtgtcgccg cttcgagccg
      901 ctcggccttg agctccagta tcagctcgaa gttctggccg aagtaacgca ggtccgcata
      961 ccactggaaa cgggcctcgc gctcgccttc cgccttgatc cacgccgtgc cgcgcttctt
     1021 gagttcggca agcccgcgat tgagcgccgc gacgttctgc gcctccgcgc gcagcagccg
     1081 cgtgaggctg aaatccccgc gcaggtcagc gtgaatgagt ccgatggcgg agagcagtcc
     1141 ggggcgcggg ggcacgacca ccttgcgcat tcccatgttg cgggcgacat cggctgcgtg
     1201 cagcggtccg gccccgccga aggcgacgag cgcgaagtcg cgcgggtcct cgccctgctc
     1261 gaccgaaatg acgcgcaccg cccccatcat gttgacgttg atgatctcga gcacgcctgc
     1321 cgccgcctga atcggatcca cgcctacggt ccgcgcgagt tcgtcgatga cggcccgggc
     1381 gcgatcggga tacaccgcca tgcgcccacc cagcagcgcg cgcggattga ggcgaccgag
     1441 cacgacgttc gcgtcggtaa ccgttgcgcg cgcgccgccc cgtccgtatg cggcggggcc
     1501 gggctccgca cccgcgctct gcgggccgac tttgagcaat ccgcccgcat ccacccacgc
     1561 gatgctgcca ccgcccgcgc cgatggtatg gatgtcgatg gtgcgggtgc ggaccggaaa
     1621 gccggccatc tggcgttcgc tcttcttcgc cggttcgccg tcgcggatca ggcagacgtc
     1681 ggtgctcgtt ccgcccatgt cgaaggtaat gagatgcgaa agaccgagcg ctttcccgag
     1741 cgcgacgctg ccgatcacac cgcccgcagg cccggaaaaa aaggtgttca ccggcgctct
     1801 gcgcaccgca cccggcgaaa ccgcacctcc gttcgactgc atgacccgtg gtgtcgccgc
     1861 aatgcccaag tccgccaccc cgcgctcgaa ccgctcgaga tagcggtcca tgatcggcat
     1921 caggctcgcg ttgaccgctg cggtagcgaa tcgctcgaac tcccgaaact ccgccagcac
     1981 ttcactcgag gtgcagagat agacctcggg ccacaacgcc tgcacgagcg cctttgcccg
     2041 ggcttcgtgg tccggattgg cataagcgtg catgaagcag atcgcaactg cctcgacctt
     2101 ctttcccttg aggacgtcga ctgcggactt caacccgtcc tcgtcgagcg gcgcgaccac
     2161 cgtgccgtca tgggcgatgc gctcgttcac ctcaatacgg caatcgcgcg cggccggcgg
     2221 catcggcttc ggcacatcga ggttgaagta atgcgggcgc cgctggcgcg caagttcgag
     2281 cacgtcgcga aagccgcgcg tggtgatgac accgctgcgc gcccacttgc cttcgagcac
     2341 ggcgttggtg gcgagcgtcg ttccgagcac cagaaaggtc acgtcggcgc gcgcgagatc
     2401 gttccggtcg aggatttctg cgacgccttc cagtatgccg cgcgcgggat cgcgcgtcgt
     2461 cgtgggaacc ttatggtagt gatagcggcc gcgatcgagg tccgcggcga cgagatcggt
     2521 gaacgtgccg ccggtgtcga tgccgacgca taccgccttc atgcgcttct tccgtagggc
     2581 gaaaacgcga tactaatgca agcgctttcg atccccaaac gcacaaccgg tatcgaagtg
     2641 cggccgcgca gccttgcacc gaaaaaaaag gcggctcgac gccgcctttt ttaatccgaa
     2701 gccgccgcga ccgcaggccg cggcgggaat tcgttactgc gtatcggttt tcttgccttt
     2761 cgccttgctc ttgccttcgg tcttcttggt ggtgctcttc ttggttttct tcgccttgct
     2821 ggccttcggc ttgtccgcct tcggctgatc ctgcgcggca ggagtggccg gctgggccgg
     2881 cgtggcgggt gtggcctgtg ctgcggcggt ggcggacgcg gcggcgaagg caaccgcgat
     2941 cagcgtggac agtaacttgc tcatgcgttt ctccctgacg ttgaaaggat catccggcaa
     3001 ctccggatag tccgataacg cgggaaccgg tgttcggttg acgcttcaat cgacccgcac
     3061 gccggttgcc ttcaccaccg gtgcccactt ggcgatctcc gcgcggatgt gcgtgccgaa
     3121 ctgctcgggc gttccgccga cgggctcgta gccgttgtcg agcatgcgtt ttctcatgtc
     3181 cggctcgccg atgatgcggt tgaacgcgtc gttgaggcgc ttgatgatcg gcgccggcac
     3241 cccggccggc gcaagcatgc cgttccacga cgtggcggaa tagcccgcga cgccggcctc
     3301 ggccatcgtc ggcagatccg gcatcccgag cgcgcgcttt tcccctgtca ccgccagcgc
     3361 gcgcaccttg cccgatttca cgaagccgac gatggatgcc ggctcgccga ggatcatcgt
     3421 gatctcgccc gagagcagcg cgaccgctgc cggcccgccg cctttgtagg gcacgtggac
     3481 gatgtcgatc ttcgccatgt tggcgaagag ggcgccggtc aagtgattga aggcgccgtt
     3541 tccgctcgag ccgtaggtga gctgacctgg ccgcttcttc gcaagcgcga ccagttcctt
     3601 cacgtttttc accggcagcg aaggatgtac gaccaggacc tgcggcacgg aagaaagcca
     3661 gatgatcggg gcgaagtcac tttccttgaa cggcatcctc gaatagatgc tggggttgat
     3721 tgcatgggtt ccggagtacg cgaacacgat tgtgtatccg tcgggcggcg acttcgccgc
     3781 catttcggcg ccgatgttgc c
//
LOCUS       DASURG010000512         4847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_293619, whole genome shotgun sequence.
ACCESSION   DASURG010000512 DASURG010000000
VERSION     DASURG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293619"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFY80_09085"
     CDS             complement(<1..421)
                     /locus_tag="VFY80_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008104.1"
                     /translation="METPQSPQDGPKLLVRGGRVYDHEGDVHAPPVADIRIAGSRIVA
                     VEAELAPLADETVMDARGKLVIPGLVNAHYHSHDVLSKGLVEQLPLEIWMLFSRLGDA
                     RGRWEEVRTRTLVGAWECLRNGVTTLQDMNNQAPLDEE"
     gene            complement(440..1420)
                     /locus_tag="VFY80_09090"
     CDS             complement(440..1420)
                     /locus_tag="VFY80_09090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008105.1"
                     /translation="MTPALLIAVPMVLFAGAVTAQMTSSAPTTFPTRPIRLVLPFAPG
                     GSTDTLVRILQPRLMQALGQTIVVDNRPGGANNVATEIVANAAPDGYTLLVASPNFAT
                     NKSLYAKLSYDPLRNFVPVTHLGIGPYFMSLHAGVPANTVSELVALAKAKPRSLSYAS
                     PGVGSASHLAMELFRMRAGVDMVHVPYKGGGPAGLAVLAGDVQLFLGTFASSIANVKA
                     GKLKAIAITSTKRTSLAPDVPTLHESGFPGFDANTWDCLVAPAGTPKAVVARLNAETV
                     KILRLPEVRELVHKMGYEATGTTPEELDRFLRKEMELWAKVIRSANIRLD"
     gene            complement(1444..2862)
                     /gene="allB"
                     /locus_tag="VFY80_09095"
     CDS             complement(1444..2862)
                     /gene="allB"
                     /locus_tag="VFY80_09095"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX6008106.1"
                     /translation="MSGNTFDTVVVGGTIAAEGEPYKADIGISNGTIAAIGLPRGHQQ
                     IDAREIIDASGKFVLPGAIDCHVHFREPGHVHKEDWLTGSRAAAFGGVTTVIDMPNTH
                     PATDSVEHFLAKKEIALRASYVDFGLYGLISDRSYHDIEAMHRAGVVGFKTYLSNSAS
                     AHIGMIGDGSLLEAFEMLAELGVRCVVHAENGEIIENRTSRLQAAGRIDPRAHADSRP
                     DVCAIEAVARSLIFAEWTGAPIHIAHEGTAAAIDLIVAAKARGVDVSVETCPQYLLLS
                     VNDLMRGGGVMRCNPPLRDPSNHERLWQALRSGEIDAIATDHAPHTPDEKRGKSIWEC
                     QCGMLGVETAMPLMMTEVARGRLSLSEYVRAAARNPAKLWRLYPRKGTIQVGSDADLV
                     IVDFEREARIDQARLHSKSKVSSWHGRDVRGLPVCTLVRGKVVAREGELCVEPGWGKY
                     VVQMPATPAPRNSPQNWIKCPD"
     gene            2941..3927
                     /locus_tag="VFY80_09100"
     CDS             2941..3927
                     /locus_tag="VFY80_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008107.1"
                     /translation="MGSLAVDVAGLALPHPLMLGSGPIAVRAVDLLAFGQVASAIVTK
                     SISAMASRGSPKPRIAKSDRDGMLNYEGGPNPGIDAFCATLQEVIPQVSCPIIGSISA
                     RTLAHGSGTEHVVEKFANAGVRAIELDFKYLYDQATGRSDFRPQDMSSIIARVRAVAR
                     VPVIAKLAYGPIELGDLAKAAEDGGAAAISAINTVFPAMRISVRTRRPLLSMNYGGLS
                     GAPIRPLAIAAIFRLYEVTNLPLIGIGGVNAAEDVVEFVMAGAGAVQVYTAAQLGGPE
                     VFPRLRADLARLLDSMGVSCLSELRGAAHGHQRAVIDRQWEAVGQSAQPEDE"
     gene            complement(4173..>4847)
                     /locus_tag="VFY80_09105"
     CDS             complement(4173..>4847)
                     /locus_tag="VFY80_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6008108.1"
                     /translation="GEACRERDILLLRDGAQSTGFVHTNVRHSKIDAIADSTQKGLLA
                     LYGVGFLYVRREWAERMKPAYLARFGVDLGDAHEASIGDFAFKLARGARRFDVGNYNF
                     VGIAAVDKSMEQLLGWGTRNIESYLRTLSHALANGFIELGLPVAGGKPGKHMAQIVTV
                     GTMSTDQYGTGDPRYNHLYEHLTSNRVKLSIRRGMLRFSLHVYNNMEDVARVLDLTKQ
                     FLRKAA"
BASE COUNT          895 a         1531 c         1479 g          942 t
ORIGIN      
        1 actcctcgtc aagcggtgcc tggttattca tgtcctgcag cgtcgtgacg ccgttgcgta
       61 gacattccca ggcaccgaca agggtacgcg tccgcacttc ctcccaccgg ccgcgcgcgt
      121 cgccaaggcg gctaaagagc atccagatct ccagcggcag ctgctcgacc agacctttgg
      181 aaagaacgtc gtgcgagtgg taatgcgcgt tcaccaagcc cggaatcacg agcttcccgc
      241 gtgcgtccat cactgtctca tcggctagcg gggcgagctc ggcctcaacc gcaacaatgc
      301 ggctcccggc gatgcgtatg tcggcgacgg gcggcgcatg gacatcgcct tcgtgatcgt
      361 aaacgcggcc gccacgcacc agcagcttcg gaccgtcctg aggagattga ggcgtttcca
      421 tgactttacc ctcgtcgatt caatcgagtc ggatgttcgc gctccggatt accttcgccc
      481 acagctccat ttccttacga agaaaccggt ccaactcttc gggcgtggta ccggtcgcct
      541 cgtaacccat cttgtgcacg agctcgcgca cctccggcaa acggaggatc ttgaccgtct
      601 cggcgttcag acgcgcaacg accgctttcg gcgtccccgc cggggcgacg aggcagtccc
      661 aggtattggc gtcgaatccc ggaaagcccg attcgtgcaa cgtcggcacg tcaggcgcga
      721 gcgaggtgcg tttcgtgctg gtgatggcaa tcgccttcag cttcccggcc ttgacgtttg
      781 cgatggagga cgcaaatgtc cccagaaaca actgcacgtc acccgccagt accgcgagcc
      841 ctgccggtcc gccgcccttg tagggcacat gaaccatatc cactccggcg cgcatacgga
      901 agagctccat ggcgagatgc gaagcggatc cgaccccggg tgacgcatag ctcaggctac
      961 gcggcttcgc cttcgcgagc gcgacgagct cggaaaccgt attggccggc acaccggcgt
     1021 ggagcgacat gaaatacggg ccaatgccga gatgagtgac cggcacgaaa ttgcgcaacg
     1081 gatcgtatga aagcttggcg taaaggctct tgttggttgc gaagttgggc gacgcaacga
     1141 ggagcgtata gccgtccggc gcggcattgg ccacgatttc agtggcaacg ttgttcgctc
     1201 cgccggggcg gttgtcgact acgatcgtct gtcccagcgc ctgcatgagc ctgggctgca
     1261 gtatccgtac aagcgtgtcc gtgctgccgc caggggcaaa cgggagaaca aggcgaatcg
     1321 gacgcgttgg aaatgtggtt ggcgcggacg atgtcatctg tgcggttaca gcgcctgcaa
     1381 acaacaccat cggcaccgcg atgagcaatg cgggtgtcat gagcctctcc ttgaggtgga
     1441 acgctagtcc ggacacttta tccaattttg gggactgttg cgaggcgcgg gcgtggctgg
     1501 catttgcacg acatactttc cccagcccgg ctcgacgcac aactcgcctt ctcgtgcgac
     1561 caccttgccg cgtacgagcg tgcagaccgg gagaccacgg acgtcgcgcc cgtgccacga
     1621 tgacactttg ctcttcgaat gaagccgagc ttggtcgatc cgcgcttcgc gttcgaaatc
     1681 gacgatcaca aggtccgcat cggagcccac ctggatcgtg cctttccttg gatacagacg
     1741 ccacagcttc gctgggttgc gcgcggccgc gcgcacgtat tccgacagcg acaaccgccc
     1801 gcgcgcgact tccgtcatca tgagcggcat cgcggtctct acgccaagca ttccgcattg
     1861 gcattcccag atgcttttgc cgcgcttctc atctggcgta tgcggcgcgt gatccgtggc
     1921 gatggcgtcg atctccccgc tgcgtagcgc ctgccacagg cgttcgtgat tcgaaggatc
     1981 gcgcagcggc ggattgcagc gcataacgcc gccaccacgc atgagatcgt tcactgaaag
     2041 cagcaggtac tgaggacacg tttcgaccga cacgtccacg ccgcgagcct tggctgcaac
     2101 gatcaggtcg atcgcggccg ctgttccctc gtgcgcgata tggatcggcg cgccggtcca
     2161 ctcggcaaat atgaggcttc gtgccactgc ttcgatcgcg caaacgtccg gccgcgaatc
     2221 ggcatgcgcg cgcggatcta tgcgccccgc cgcctgcagc cgcgaggtgc gattctcgat
     2281 gatctctccg ttctcggcgt ggaccacgca ccgcacaccc agctcggcca gcatttcgaa
     2341 cgcttcgagc aatgacccgt caccgatcat cccgatatgc gcgctggcgg aattgctgag
     2401 ataggtcttg aaaccgacga cccctgcacg gtgcatcgct tcgatgtcgt gatacgagcg
     2461 atcggaaatg agcccgtaaa gcccgaagtc cacatagctc gcgcgaagcg cgatctcttt
     2521 cttggcgagg aaatgctcta cgctgtcggt agcggggtgc gtgttcggca tgtcgatgac
     2581 cgtcgtcaca cctccgaagg cggctgcacg cgagccggtc agccaatcct ccttgtgcac
     2641 atgaccgggc tcacggaagt ggacgtggca atcgatcgcg ccaggcagta cgaacttgcc
     2701 agacgcatcg atgatctcgc gcgcgtcgat ctgttggtgg ccgcgcggca ggccgatcgc
     2761 ggcgatagta ccgttactga taccgatgtc ggccttgtac ggctcgcctt cggcggcgat
     2821 agtgccgccg acaactactg tatcgaacgt gttgccggac acggccccat ctcctcttgt
     2881 tggactgcaa ctgcgataga ctagcgatta cattatccta cgaagttcgg acgctgccga
     2941 atgggaagct tggctgtcga tgtcgcgggg cttgctctgc cgcatcctct catgctgggt
     3001 tcaggaccaa tcgcggtacg cgccgtcgac ctgctcgcct tcgggcaggt ggcctctgca
     3061 atcgtgacca agtcaatctc ggcgatggct tcgcgcggga gcccgaagcc ccgcattgca
     3121 aaatccgacc gcgacggcat gctcaactac gaaggtggac cgaacccagg catcgacgcg
     3181 ttttgcgcca cgctgcagga agtaatcccc caggtgtcct gtccgatcat tggctcgatt
     3241 tcggccagga cccttgccca cggctccggc accgagcacg tggtggagaa attcgcaaat
     3301 gccggcgtgc gcgcgatcga gctcgatttc aagtatctct atgaccaggc cacgggccgc
     3361 agcgattttc gcccgcaaga catgtcttcg atcatcgcac gcgtgcgggc ggtcgcgcgt
     3421 gttccggtta ttgccaagct cgcgtacgga cccattgagc tgggtgatct cgcgaaagcc
     3481 gccgaagacg gcggtgcggc agcgatttcc gcgatcaaca cggtgtttcc ggccatgcgt
     3541 atcagcgtcc gcacacggcg gccattgctc tcgatgaatt acggcggctt gtcgggcgcg
     3601 ccaatacgac cgctcgcgat tgccgccatc tttcgcctct atgaagtgac gaacctgccc
     3661 ttgatcggca ttggaggggt gaacgcggcc gaagacgtcg tcgagttcgt catggctggt
     3721 gcgggcgcgg ttcaggtcta cacagctgct cagctcggtg gccccgaggt cttcccgaga
     3781 ttgcgagccg atctcgcgcg cctgctcgac agcatgggcg tatcgtgcct gtccgaactg
     3841 cgtggcgctg cgcacggcca tcaaagagcc gtcatcgacc gtcagtggga ggctgtgggc
     3901 caaagcgcgc agccggagga tgaatgagca cggacgcata ggtcggcagc tgtttcggac
     3961 ttctgccgcc gggcccgtgt ttgggttgga gcgcgtactt ctcctcgagc gtgactgcga
     4021 ctacatggcg cgacacgcaa gtctcgatcg cgctagatgt cggcgcgaca ccacggcacc
     4081 acgcgaatta tgagatcgcg tgccggccgc tgttccgatt catcgcggga tcccgatcgg
     4141 ctaccaaggg ccggtagccg cgtcagcaca gttcaggccg ctttgcgcag aaactgcttc
     4201 gtaagatcga gcacgcgcgc cacgtcttcc atgttgttgt agacgtgcag cgaaaagcgc
     4261 agcataccgc ggcggatcga aagcttcaca cggttcgacg taagatgctc gtaaaggtgg
     4321 ttgtaacgcg ggtcgccagt gccatattgg tcggtgctca tcgtaccaac cgtaacgatc
     4381 tgcgccatgt gcttgcccgg cttgccaccc gcgactggga gccccagctc gatgaaacca
     4441 tttgcgagcg cgtggctgag cgtgcggaga taggattcga tgttgcgcgt accccatccc
     4501 agaagctgtt ccatcgactt gtccacggct gcaattccga caaagttgta attgccgacg
     4561 tcgaagcggc gcgcgccccg tgccagcttg aaagcgaaat cgccgatgct cgcttcgtgc
     4621 gcgtcgccga gatcgacacc gaaacgcgcg aggtacgccg gtttcatgcg ctcggcccat
     4681 tcccggcgta cgtataggaa tcccacgcca tagagcgcga gcaggccctt ctgcgtcgaa
     4741 tctgcgatcg cgtcgatctt ggagtggcgg acgttggtgt ggacgaagcc cgtcgactgc
     4801 gcgccatcac gcaggagcag gatatcgcgc tcccggcacg cttcacc
//
LOCUS       DASURG010000513         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_905030, whole genome shotgun sequence.
ACCESSION   DASURG010000513 DASURG010000000
VERSION     DASURG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905030"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFY80_09110"
     CDS             complement(<1..594)
                     /locus_tag="VFY80_09110"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX6008109.1"
                     /translation="MKSLNSVVRGHAPRSLLGIAIATAMLWSAGALAQQAVTLRLGHV
                     GFPGSLFDISANKFAKDVNEALKGKVEVRVFHSSQLGSDEAMIKGLRVGAPEMQVPST
                     IMSTVDQRHGVFEMPYLIVSRAHMKKVSESREVQKVLVDPLPARGVRVLAFWENGFRH
                     ITNNVRPIVRPEDLKGIKLRVPGGVWRVKMFQAYGANP"
     gene            complement(702..1550)
                     /locus_tag="VFY80_09115"
     CDS             complement(702..1550)
                     /locus_tag="VFY80_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX6008110.1"
                     /translation="MDEAQRPMSLRERIGIDLGRKIRLEEGIAWATANDVRYIDIQLD
                     TAANALTTFDDRRAAGIRTACEKAGVHLGLHTLSAVNVAEYSPYVSDAVDAYLKSYID
                     IYPKLGAEWIVVHAGYHFSSDKPLRMQAGLERLKRIADYAERKGALILLENLNKEPED
                     AEVHYLAHTLEEWRYYWSLLSSPAVRLSFTVNHAHLVPEGVDAFVAALDMSLVGEVRL
                     ADCHRNGHEVHLRPGEGNVDFAKVLRSIEDKGFKGHYTNAFGSLEDMLAGREYLVVKA
                     REAGLA"
     gene            complement(1526..1993)
                     /locus_tag="VFY80_09120"
     CDS             complement(1526..1993)
                     /locus_tag="VFY80_09120"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX6008111.1"
                     /translation="MTPNQQHVEYMKRALEQARETGERGNRPVASIIVRDGAIIGEGR
                     NTILSDFDPSAHAEIAAIRQACGKLKTVDLSGSTLYSTLEPCPMCFWAMQEAKVGRLV
                     LGGRYAGIGRTDMGRYTVESFLEFTGRSLEVVTGVLASECEAVRLEWMKRNAR"
     gene            complement(1995..2960)
                     /locus_tag="VFY80_09125"
     CDS             complement(1995..2960)
                     /locus_tag="VFY80_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008112.1"
                     /translation="MRILLTLLALFAPLVFSGGAKAQAYPAKAIRFVVPFAPGGGNDI
                     LGRVVAQKLNEGFGVPVVVDNRGGAGGTIGTDIVAKSPPDGYTLLINNVSLAVNVTLY
                     AKLPYDTLKDLEPVSLAGRQPNILVVHPSLPVKSVKELLALARGKPAALSFASGGIGS
                     SSHLSAELLKYMTKVDIVHVPYKGMGPALIDLVSGQTQFAMATMASALPHIRNGRMRA
                     LAVSTAKRVAVVPDVPTMMEAGVRDFEYSTWYGILVPAGTPQPVIVRLSAELAKALSA
                     RDVAERFGAQGVEPVSTTPQEFKSFLVKEVKRWAPVIKAAGMQVN"
     gene            complement(2971..3939)
                     /locus_tag="VFY80_09130"
     CDS             complement(2971..3939)
                     /locus_tag="VFY80_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008113.1"
                     /translation="MLRVVSTLLVAVGLAGSAGLAVAQNYPNRPIRMVVPFAPGGGSD
                     ISGRVLADGLTEALGQTIVVDNRPGAGSILGCDIVAKANPDGYTTLLGNISMAFNTAI
                     YKKLPYDAIKDFSPISLVTDQPNILVAHPALPAKTFKEFIALATAQPGKLTYGSAGMG
                     SGTHLAMEMLLMSRKLDLVHVPYKGTGPALTALLGNQISVFFSTYASALPHVKASRLR
                     AYAVTSAKRTTTLPDIPTVAESGFPGYEYSTWYGFLAPAGTPRAIVEKLNKAAVSVLK
                     SDKTHERYITQGMEPIPSTSAEYVKYLKSEIDKWTKVVRAAKLPLQ"
     gene            complement(4063..>4253)
                     /locus_tag="VFY80_09135"
     CDS             complement(4063..>4253)
                     /locus_tag="VFY80_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HEX6008114.1"
                     /translation="RDRVFRIGHLGAFNDLMLMGTLAGVEMGLAIAAVPHRKGGVAAA
                     MEYLSGVASSAPARQKAA"
BASE COUNT          747 a         1358 c         1332 g          816 t
ORIGIN      
        1 cgggttcgcg ccgtaggcct ggaacatctt cacccgccag acgccgcccg gcaccctcag
       61 cttaatgccc ttcaggtcct cgggccgcac gatcgggcgc acgttgttgg tgatgtggcg
      121 gaatccgttt tcccagaagg cgagaacgcg caccccgcgc gcgggcaacg gatcgacgag
      181 caccttctgc acctcgcggc tctccgacac ttttttcatg tgcgcccggc tcacaatcag
      241 atacggcatc tcaaagacgc cgtgtcgctg atcgacggtg ctcatgatcg tcgagggcac
      301 ctgcatttcc ggtgcgccga cccgcaggcc cttgatcatc gcctcgtcgg aaccgagctg
      361 actcgagtgg aacacgcgca cctcgacctt gcccttcagc gcctcgttga cgtccttcgc
      421 gaacttgttg gcggaaatat cgaaaagcga gccggggaag ccgacgtgcc ccagacgcag
      481 cgtgaccgcc tgctgagcaa gcgcgcctgc cgaccagagc atcgccgtcg cgatcgcgat
      541 gccgagcaac gagcgcggcg catgtccccg cacgactgaa ttcagagatt tcatgatcct
      601 cctccttgtt ccgttaaagg tgtgctgaaa tggtgcgggc tggagattgt ccttgttgcc
      661 cgccccgaac aataacttta cccgatttac aatgtcatgc gctacgcgag tccggcctcc
      721 cgcgctttta caaccagata ttcgcgcccg gcaagcatgt cctccagcga gccgaacgca
      781 ttggtgtagt gacccttgaa ccccttgtcc tcgatgctgc gcaagacttt ggcgaaatcg
      841 acgttgcctt cgccgggcct cagatgcact tcgtggccat tgcggtggca gtcggcgagc
      901 cgaacttcgc ccacgagact catgtcgagc gccgcgacga acgcatcaac accctcggga
      961 acgaggtgcg cgtggttgac ggtgaacgag agccgcaccg caggcgaaga gagcaggctc
     1021 cagtaatacc gccactcttc gagcgtatgg gcgagatagt gcacctcggc gtcctcgggc
     1081 tccttgttga gattctcgag aaggattagc gcgcccttac gttcggcgta gtcggcgatc
     1141 cgtttgagcc gctcaaggcc cgcctgcatc ctgagcggct tgtcgctcga aaaatgataa
     1201 cccgcgtgca cgacgatcca ttcggcgccg agcttgggat agatgtcgat gtagctcttg
     1261 agatacgcgt ccaccgcatc gctcacgtag ggggagtact ccgccacgtt gacggcggaa
     1321 agcgtatgga gcccgagatg gacgcccgcc ttttcgcagg cggtgcggat tccggcggcg
     1381 cgccgatcat cgaaagtcgt cagcgcattc gccgcggtgt cgagctggat gtcgatgtag
     1441 cggacgtcat ttgctgttgc ccaggcgatg ccttcctcga ggcggatctt gcgcccgagg
     1501 tcgatgccga tacgctcgcg caggctcatc gggcgttgcg cttcatccac tccaggcgca
     1561 ccgcctcgca ttccgacgcc aacacgccgg tcacgacctc gagtgagcgc ccggtaaatt
     1621 cgagaaacga ttcgacggtg taacggccca tgtcggtgcg tccgatgccg gcataacgcc
     1681 cgcccagtac gagacggccg accttcgctt cctgcatggc ccagaaacac atcgggcagg
     1741 gctcgagcgt ggaatagagc gtgcttccgg agagatccac cgttttcagc tttccgcacg
     1801 cctgccggat ggcggcgatt tccgcgtgcg cgctcggatc gaaatcgctg agaatggtgt
     1861 tgcggccttc gccgatgatc gcaccgtcac gcacgatgat ggaggccaca ggtcgattgc
     1921 cgcgctcgcc ggtctcgcgc gcctgctcga gcgcgcgctt catgtactcg acgtgttgtt
     1981 ggttcggcgt catgtcagtt cacctgcatg ccggcggcct tgatgaccgg tgcccagcgt
     2041 tttacttcct tcacgaggaa ggacttgaac tcctgcggcg tcgtggacac gggctcgacg
     2101 ccttgcgcgc caaagcgctc ggccacgtcg cgcgctgaga gcgctttcgc gagttcggcg
     2161 ctgagccgca cgatcacagg ctgcggggtg ccggcgggca cgagtatgcc gtaccatgtg
     2221 ctgtattcga aatctcttac accggcttcc atcatcgtcg gcacgtcggg cacgacggcg
     2281 acccgttttg cggtcgatac tgcgagcgcg cgcatgcggc cgttgcgtat gtgcggaagg
     2341 gcggaggcca tggtcgccat cgcgaattgc gtttgcccgc tcacgaggtc gatcagcgcg
     2401 ggccccatgc ccttgtacgg cacgtggacg atgtcgacct tcgtcatgta tttgagcagc
     2461 tccgcggaga gatgagagct gctcccgatg ccgcccgatg cgaacgaaag tgcggccggc
     2521 ttcccgcggg cgagggcgag cagctccttg accgatttca ccggcagcga aggatgaacg
     2581 accagtatgt tgggctggcg tccggcgagg ctcaccggct ccagatcctt cagcgtatcg
     2641 taaggaagct tcgcgtagag cgtcacgttg accgcgaggc tcacgttatt gatgaggagg
     2701 gtgtagccgt cggggggcga cttggcgacg atgtcggtgc cgatcgtgcc cccggcgccg
     2761 ccacggttgt ccacgaccac cggcacgccg aagccctcgt tcagcttttg tgccaccacc
     2821 cgccccagga tgtcattgcc gccccccggg gcgaacggca cgacaaaacg gatcgccttg
     2881 gcgggatagg cctgcgcctt ggcgccgccg gagaagacga gcggcgcgaa gagcgcgagc
     2941 aaagtcagca aaatgcgcat cgtggacctc tcattgcagc ggcagcttgg ctgctcgcac
     3001 caccttcgtc cacttgtcga tctcggactt gaggtacttc acgtattcag ccgaagtgga
     3061 aggaatcggc tccatgccct gcgtgatgta gcgctcgtgc gtcttgtccg acttgagcac
     3121 gctcaccgcg gccttgttga gtttctccac gatcgcgcgg ggcgtgcctg ccggggcgag
     3181 aaaaccgtac cacgtgctgt actcgtaacc cggaaatccg gattccgcga cggtcggtat
     3241 atcgggtagc gtcgtcgtgc gcttggcgct cgtgacggcg tacgcccgaa gccggcttgc
     3301 cttcacgtgc ggcaacgccg aagcgtaggt cgaaaagaac accgaaatct ggtttccgag
     3361 gagcgccgtg agcgcggggc ccgtaccctt gtacggtacg tgcacgagat cgagtttgcg
     3421 cgacatgagc agcatctcca tcgcgagatg cgttccggag cccattccgg ccgagccgta
     3481 ggtgagcttg ccgggttgtg cggtggcgag cgcgatgaat tccttgaacg tcttcgcggg
     3541 aagcgcggga tgcgcgacca gtatgttggg ctgatcggtc acgagcgaga tcgggctgaa
     3601 gtccttgatc gcgtcatacg gaagtttctt gtagatcgcc gtgttgaacg ccatgctgat
     3661 gttgccgaga agcgtggtgt agccgtcagg gttcgccttg gcgacgatat cgcagccgag
     3721 gatgctgccc gcgcccgggc ggttgtccac gacgatcgtc tggcccagcg cttcggtcag
     3781 gccgtccgcc agcacgcggc ccgatatgtc gctgcccccg cccggagcga acggcaccac
     3841 catgcgaatc ggccggttgg gatagttctg ggcaaccgca aggccagcgc ttccggcgag
     3901 acccaccgcg accagcagag tcgacaccac acgcagcatc tctttcctcc tctttttagg
     3961 tatgaattga cgtcgaaacg gcattgcgcg cgccgccgca accgcccgcg cgcgtgaaac
     4021 tttcagcgat gatcgccggc ctgtcaaccg gcgcacgccg cttcaggccg ctttctgccg
     4081 cgctggagcg ctcgaggcta cgccggatag gtactccatc gccgcggcaa ctccgccctt
     4141 gcggtgtggc acggcggcaa tcgcaagccc catctccacg cccgcgagcg tgcccatcag
     4201 catgagatcg ttgaaggcgc ccagatgtcc gatgcgaaac actctgtcgc gca
//
LOCUS       DASURG010000514         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1109242, whole genome shotgun sequence.
ACCESSION   DASURG010000514 DASURG010000000
VERSION     DASURG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109242"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..502)
                     /locus_tag="VFY80_09140"
     CDS             complement(74..502)
                     /locus_tag="VFY80_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6008115.1"
                     /translation="MKTVRQLLAVKGSTVHSVAPDARVYEALRLMAEKEVGALVVLEG
                     GTLVGILSERDYARKVILHGKSSHDIPVRGIMTENVVTVHPGQTDDQCMALMTERRIR
                     HLPVLDNGRLVGLVSIGDLVKEVIAEQEQMIRQLETYIHS"
     gene            complement(606..>2255)
                     /locus_tag="VFY80_09145"
     CDS             complement(606..>2255)
                     /locus_tag="VFY80_09145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6008116.1"
                     /translation="HQFLVMFVIGGMVLSAAGVLSTVKRVFYGFTLPAIVPLLMTLFL
                     QGHAPHVQMGMLGVAYTVVILAFAAEMRANVLASTHAQEELRQATQRLQALINASPLA
                     IVVRDAEGRIERWNPAAERMFGWTEAEVRNRMVPWYPPGKEEEGYRFRAVIMRGEAFS
                     DVEAVRLRKDGTPLMVSLSGAPMLNDAGKAVGVLVIVADITVRKRIEMRQELQNEITA
                     LLAEPRSSVSETLVKVIQAFCEKLGWVCGSRRVLDPEINSLRCAEAWGNGDPTVMEFV
                     EQTRALLTPSTSKHSGVTRRVWDSGQPMWITDIQADPQLKRGEAARKAGLHTALAFPV
                     MIGEELYGVMEFFAQESRQQDPGMLQFTRQLGSQIGQFIARKQAEHDLRFVATHDPLT
                     ALPNRTMFAERLSQALAQARRYNRRVALLFVDLDGFKNVNDSRGHDVGDALLKEIAVR
                     LRVCLREGDVIGRMGGDEFVVLIEEFQDAEHLAPIAQKILDTVARPVTVRGEDCYVTS
                     SIGISAYPQDGRDSQTLLKSADSAMYRAKEQGKNRYAFHSI"
BASE COUNT          401 a          764 c          658 g          432 t
ORIGIN      
        1 aaagtaacgg cgtagactcg cttcaccaaa aaagccccgg cacacggggc ttttttttgg
       61 ggtcgtgtcc gcgtcagctg tggatgtagg tctcgagctg acggatcatc tgctcctgct
      121 cggcaatcac ttccttcacc agatcgccga tcgataccag ccctacaagc cttccgttat
      181 ccagcaccgg aagatgccgt atgcgccgct cggtcatgag cgccatgcac tgatcgtcgg
      241 tctgtccggg atgcaccgtc accacgtttt ccgtcatgat cccgcgcacc ggaatgtcat
      301 gtgaggactt cccgtgcagt atcaccttcc gggcatagtc ccgctccgaa aggatgccga
      361 cgagcgtccc gccctcgagc acgacgagcg cacccacttc cttttccgcc atgagcctga
      421 gcgcttcata gacgcgcgca tcgggcgcca ccgagtgaac cgtactgccc tttaccgcaa
      481 gcaactgcct gaccgttttc atagccgcac tccttgccgc cgcgccgatc ccccgcgaaa
      541 tttctccttg tgccgggtca gtgtagccaa tttccggagc cccataaaga ttcacggccc
      601 gcgaactaga tggaatggaa tgcgtaacgg tttttgccct gctctttggc gcggtacatc
      661 gcgctgtccg cgctcttgag cagcgtttgc gaatcgcgcc catcctgcgg gtacgcgctg
      721 atgccgatgc tcgacgtgac gtagcagtcc tcgcctcgca ccgtgaccgg ccgcgccacg
      781 gtgtcgagga ttttctgcgc gatcggggcg agatgctcgg catcctggaa ttcttctatc
      841 agcaccacga actcgtcgcc gcccatgcgg ccgatgacgt ccccctcgcg caggcagact
      901 ctcaggcgca cggcgatctc cttcaacagg gcgtccccga cgtcgtgacc gcggctgtcg
      961 ttcacgttct tgaaaccgtc cagatcgacg aagagcagtg cgacccgtcg gttgtagcgc
     1021 cgggcttgcg cgagcgcctg actcaggcgc tccgcgaaca tcgtacggtt cggaagcgcg
     1081 gtgagcgggt cgtgcgtcgc cacgaacctg agatcgtgct ccgcctgctt gcgcgcgatg
     1141 aactggccga tctggctgcc aagctgtcgg gtaaattgca gcatgcccgg gtcctgctgg
     1201 cgcgattcct gtgcgaagaa ctccatgacg ccgtacagct cttcgccgat catcaccgga
     1261 aaggcgagcg ccgtatgcag cccggccttg cgagccgcct cgccgcgctt cagttgggga
     1321 tcggcctgaa tgtccgtgat ccacatcggc tggccgctgt cccacacgcg tcgcgtcacg
     1381 ccggaatgct tgctggtcga cggtgtcaga agcgcgcggg tctgctccac gaattccatc
     1441 accgtggggt cgccgttccc ccaagcttcc gcgcagcgca gggaattgat ctcgggatcg
     1501 agcacgcggc gtgagccgca gacccagccc agtttctcgc aaaacgcctg gatcactttc
     1561 acgagcgtct cgctcaccga gctgcgcggc tcggccagca gcgcagtgat ctcattctgc
     1621 aactcctgcc gcatttcgat gcgcttgcgg acagtgatgt cggcgacgat gacgaggaca
     1681 cccaccgcct tgcccgcgtc gttcagcatc ggtgccccgg agaggctcac catgagcggc
     1741 gtgccgtcct tgcgcagccg caccgcttcg acgtccgaaa acgcctcacc gcgcatgatg
     1801 acagcgcgga agcgatagcc ctcttcctct ttcccgggcg gataccacgg caccatcctg
     1861 ttgcgcacct ccgcttcagt ccaaccaaac atgcgctcgg ccgccgggtt ccagcgttcg
     1921 attcggccct cggcgtcgcg aacgacgatg gcgagcggag acgcgttgat gagcgcctgt
     1981 agtctttgcg tagcctgacg cagctcttcc tgcgcgtgcg tggaagccag cacgttggcc
     2041 ctcatctcgg ccgcaaaggc gagaatgacg accgtgtacg ctacgcccag catgcccatc
     2101 tggacgtgcg gcgcatgacc ctggaggaac agcgtcatga ggagcggaac gatcgcaggc
     2161 agcgtgaagc cgtagaacac acgcttcacc gtcgacagca cgccggctgc ggacaggacc
     2221 atgccaccga tgacgaacat cacgagaaac tggtg
//
LOCUS       DASURG010000515         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_294087, whole genome shotgun sequence.
ACCESSION   DASURG010000515 DASURG010000000
VERSION     DASURG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294087"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFY80_09150"
     CDS             <1..266
                     /locus_tag="VFY80_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6008117.1"
                     /translation="PTIAETVPGYSVSTWYGAMVPAKTPREIVVRLNQEMVKALALPD
                     VKERLASLGADIVASSPEQTASFFRSELAKYTKVAQAAGIRAE"
     gene            392..1648
                     /gene="otnK"
                     /locus_tag="VFY80_09155"
     CDS             392..1648
                     /gene="otnK"
                     /locus_tag="VFY80_09155"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX6008118.1"
                     /translation="MSVLLGCIADDFTGATDLANTLTRCGMTSVVLPGVPGADFAVPD
                     ADAIVVALKSRSNPPADAVRMSLEARDWLARAGARQYYFKYCSTFDSTDAGNIGPVAE
                     ALLAALDTTFTVACPAFPTNGRTVYQGYLFVGERLLSESSMRHHPLTPMTDPSLVRVL
                     QRQCNGRIGLIPHEVVRDGPAAIRCARERLARDGARIAVVDALTDADLVAIGEACAEL
                     PLLTGGSGLALGLPRNFRRAGALEPRAPAALAPIEGHAAVLAGSCSAATQRQVERMKE
                     AYAAFEIDPIELHTNGKAVRDALEWARLHVGASPILLSSTADENAVARAQAALGRERA
                     GELIERAMGEIAKGLVAMGVRRLVVAGGETSGAVVHALGIRGLRIGEEIDPGVPWTAS
                     LDEPPLALALKSGNFGSDDFFLKALT"
     gene            1645..2274
                     /gene="otnC"
                     /locus_tag="VFY80_09160"
     CDS             1645..2274
                     /gene="otnC"
                     /locus_tag="VFY80_09160"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HEX6008119.1"
                     /translation="MNENALREHMVEIGKSLYERGLAHGSAGNLSVRLDDGWLMTPTN
                     SCLGRLDPSRLSRLDPSGRLLAGDRPSKETFLHLAMYRERACGAVVHLHSVHAVAVSC
                     LADVDPESVFPPLTAYAIMQLGRLALAPYYPPGDESLAEAVRNLAGKHHAILLANHGP
                     VVAGATLDAAVNAIEELEQTAKLMLLLRGQPLRLLTSEQVAELNRRFSN"
     gene            2295..3290
                     /locus_tag="VFY80_09165"
     CDS             2295..3290
                     /locus_tag="VFY80_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6008120.1"
                     /translation="MSLAAFVAVLAAVPAAAQSTLDAVRGRGHVQCGVNTGLAGFSQP
                     DSKGVWRGIDADFCRAVAAAVFGDPNKVRFTPLTSQQRFTALQAGEIDLLARNTTWSI
                     TRDTALGLNFVGVNYYDGQGFIVPKKLNVKSVKELNGATICVQPGPTNELNVADYFRA
                     HGMKFKPVVIEKLEEVLNAYFAGRCDVYTTDVSGLAAIRASRAPNAAEHVILPEVISK
                     EPLGPAVRHGDDRWFDIVKWTLFAMIEAEELGLTSKTIDRHLKSANPSVLRFVGASGE
                     IGKALGLDNRWAYNVVKHVGNYGESFERNLVPLGFERGLNRLWSQGGLMYAPPIR"
     gene            3301..>4320
                     /locus_tag="VFY80_09170"
     CDS             3301..>4320
                     /locus_tag="VFY80_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6008121.1"
                     /translation="MKFSFHDGRLRALAYQAAALGTTLALAWFLVGNTLENLAERNIA
                     SGFAFLGREAGFAIGETTFLSYSAADTYLRALAVGLANTARVAVIGIFAATALGTLIG
                     LARLSRNWLVARVAAGYVEFVRNVPLLVQLFFLYAVMTEYLPAPRAAFQPLPGLYLSN
                     RGIFLPVPEAHPVYGWMLIACLAGLIAALLLRRWAATHRVAGRRLPLLGIGLGLALVP
                     PALAYLLAGAPTALDVPKLAGFNFTGGMAISPEFATLAGGLILYTAAFIAEIVRAGVQ
                     SVPSGQSEAAYALGLSSSRTLRHVVLPQALQAIVPPLTNQYLNLTKNSSLAVAIGYPD
                     LVSIAN"
BASE COUNT          692 a         1431 c         1428 g          769 t
ORIGIN      
        1 tgccgaccat cgcggaaacc gtgccgggct attcggtgag cacgtggtac ggcgcgatgg
       61 tgccggcgaa gacgccgcgc gagatcgtgg tgcgcctcaa tcaggaaatg gtgaaggccc
      121 tcgcgctgcc cgatgtgaag gagcgactcg cctcgctcgg cgcggacatc gtggcctcga
      181 gccccgagca gaccgcgtcg ttcttccgct ccgagctggc gaagtacacc aaggtcgcgc
      241 aggcggccgg gatccgggcc gagtaagcgc tgaaggaagc gccgggcacg atgaagcaag
      301 ccgtcctgcc ggcgaaagcc gacgttcgca gccggaaccg gattccgact ttggtcggaa
      361 tggcgatacg accataaggg cgaacagcac ggtgtccgtc ctgctcggtt gcatcgccga
      421 tgacttcacg ggcgcgacgg atctcgccaa tacgctgaca agatgcggca tgacgagcgt
      481 cgtgctgccc ggcgtgccgg gtgccgattt cgccgtgccg gacgccgacg cgatcgtcgt
      541 cgcgctcaag tcgcgttcga acccgcctgc cgatgcagtg cggatgtcgc tcgaggcccg
      601 cgactggctc gcgcgtgcgg gcgcacgcca gtactacttc aagtattgtt cgaccttcga
      661 ttcgaccgat gcagggaaca tcgggccggt ggccgaagcg ctgcttgccg ccctcgatac
      721 cacgttcacg gtggcgtgcc ccgcgtttcc cacgaacggg cgaaccgtgt accagggcta
      781 tctcttcgtc ggcgagaggc tcctgtccga atcgagcatg cgccatcatc cgctgacgcc
      841 catgaccgat ccatcgctcg tccgcgtgct ccagcggcag tgcaacggtc gcatcggatt
      901 gatcccgcac gaggtcgttc gggacggacc ggcggcgata cgctgcgctc gtgaacgtct
      961 cgctcgcgat ggcgcgcgca ttgcggtcgt cgatgcgctg accgacgcgg atcttgtcgc
     1021 catcggcgag gcgtgtgccg agctgccgct tctcaccggc ggatcggggc tcgcgctcgg
     1081 cctgccgcga aactttcggc gtgcgggcgc gctcgaacct cgcgcaccgg ccgcgctcgc
     1141 gccgatagag ggtcatgcgg cggtactggc gggaagctgc tcggctgcga cgcagcggca
     1201 ggtcgagcgg atgaaggaag catacgccgc cttcgagatc gacccgatcg aattgcacac
     1261 gaacggcaaa gcggtgcgag acgctctcga atgggcgcgc ctgcatgtgg gagcctcgcc
     1321 cattctgttg tcctcgaccg cagacgagaa cgcggtcgca cgggcgcagg cagcgctcgg
     1381 acgcgagcga gccggagaac tcatcgagcg cgcaatgggc gagatcgcga aggggctcgt
     1441 cgcgatgggc gtgcggcggc tcgtcgtcgc cgggggcgag acctccggcg ctgtcgtgca
     1501 cgcgctcggc atccggggtc tgcgcatcgg ggaagagatc gaccccggcg tgccgtggac
     1561 ggcgagcctt gacgagcccc cgcttgcgct tgcgctcaag tcgggcaatt tcggcagcga
     1621 cgatttcttt ctgaaggcgc tgacatgaac gaaaacgcgc ttcgcgagca catggtggag
     1681 atcggcaagt cgctctacga gcgcggtctc gcacacggtt ccgccggaaa tctgagcgtc
     1741 agactcgacg acggctggct catgacgccc accaattcct gcctcggccg cctcgacccg
     1801 tcacgcctct cgcggctcga cccctcgggc aggctgctcg ccggcgacag gccctcgaag
     1861 gagacgttcc tgcaccttgc gatgtaccgc gagcgggcat gtggcgcagt ggtgcatctg
     1921 cattcggtac atgcagtggc ggtatcgtgc ttggccgacg tcgatccgga gagcgtcttc
     1981 ccgccgctca cggcttacgc catcatgcag ctcggccggc tcgcgctcgc gccctattac
     2041 ccgccgggag acgaatcgct ggcagaagcg gtgcgcaacc tcgccggcaa gcaccatgcg
     2101 attctcctcg cgaatcacgg accggtcgtt gcaggcgcga cgctggacgc agcggtgaac
     2161 gccatcgagg agctcgagca gaccgcaaag ctcatgctgc tgctccgcgg tcagccgctg
     2221 cgactgctca cctcagagca ggtcgccgag ttgaaccgcc gtttctccaa ctgagactcg
     2281 ctatgcgctc atcgatttcg ctcgcggcct tcgttgccgt gctcgccgca gtgcctgccg
     2341 ccgcgcaatc gacgctcgac gccgtgcgtg ggcgcggtca cgtgcagtgc ggcgtcaaca
     2401 ccgggctcgc cgggttttcg cagccggatt ccaaaggcgt ctggcgcggg atcgacgcag
     2461 acttctgccg tgccgtggcg gcggcggtat tcggcgatcc gaacaaggtg cgcttcacac
     2521 cgctcacctc tcagcagcgc tttacggcgc tccaggcggg cgagatcgat cttctcgcac
     2581 gcaacacgac ctggagcatc acgcgcgaca cggcgctcgg gctcaacttc gttggggtga
     2641 attactacga cgggcagggc ttcatcgtcc cgaagaagct caacgtgaag agcgtgaagg
     2701 aactgaacgg ggcgacgatc tgcgtgcagc cgggacccac gaacgagctc aatgtggcgg
     2761 actatttccg cgcgcacggc atgaagttca agccggtcgt gatcgagaag ctcgaggaag
     2821 tgttgaacgc ctacttcgcg ggacggtgtg acgtctacac caccgacgta tccggactcg
     2881 cggcgatccg cgcttcgcgc gcgcccaatg cggccgaaca tgtgatcctg ccggaggtca
     2941 tctccaagga accgctcggc ccggcggtgc ggcacggtga cgaccgctgg ttcgacatcg
     3001 tgaagtggac gctcttcgcc atgatcgaag cggaggagct ggggctcact tcgaaaacga
     3061 tcgatcggca tttgaaaagc gccaatccct cggtgctgcg cttcgtcggg gcgagcggcg
     3121 aaatcggcaa ggcgctcggc ctcgacaacc gctgggccta caacgtggtg aagcacgtcg
     3181 gcaactacgg tgagagcttc gagcgtaacc tcgtgccgct cggcttcgag cgcgggctca
     3241 accggctctg gtcgcagggc gggctcatgt acgcgccccc catccggtag cgtctcgggc
     3301 gtgaagtttt cgtttcacga cggacgcctg cgcgcgctcg cctaccaggc ggccgcgctg
     3361 ggcacgacgc tcgcgctagc gtggtttctc gtggggaata cgctcgaaaa cctcgccgaa
     3421 cgcaatatcg cgagcggctt tgcgttcctc gggcgcgagg ccggttttgc catcggcgag
     3481 acgacgtttc tctcctacag tgcagcagac acgtatctca gagcgctcgc ggtcgggctc
     3541 gcgaacactg cgcgcgtcgc ggtcatcggc attttcgccg cgacggcgct cggaacgctg
     3601 atcgggcttg cgcgtctttc gcgcaactgg ctcgtcgcgc gcgttgcggc cggctacgtc
     3661 gaattcgtgc gtaacgtccc gttgctcgtg cagctcttct tcctgtatgc cgtgatgacg
     3721 gaatatcttc cggcgccgcg cgccgcattc cagccgcttc ccggcttgta cctctcgaac
     3781 cggggcatct ttcttccggt tccggaggcc catcccgtct acggctggat gctcattgca
     3841 tgcctggcag gcttgatcgc agcgctgctc ttgaggcggt gggctgcgac gcatcgcgtc
     3901 gccgggcggc ggctgccgct gctcgggatc ggtctcggtc tcgccctcgt gccacccgcg
     3961 ctcgcttatc tcttggccgg agcgccgacc gcgctcgacg ttccgaagct cgcgggcttc
     4021 aatttcacgg gcggcatggc gatctcgccg gaattcgcca cgctcgcggg cgggctcatc
     4081 ctgtacaccg ccgctttcat cgcggaaatc gtgcgcgccg gcgtgcagtc ggtaccgtcc
     4141 ggccagtccg aggccgcgta tgcgctggga ctgtcgagca gccgaacgct gcgccatgtg
     4201 gtgctaccgc aggcgctgca ggcgatcgtg cctccgctca ccaatcagta tctcaatctc
     4261 acgaagaaca gttcgctcgc ggtcgcgatc ggctaccctg atctcgtttc catcgccaac
//
LOCUS       DASURG010000516         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_294146, whole genome shotgun sequence.
ACCESSION   DASURG010000516 DASURG010000000
VERSION     DASURG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294146"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFY80_09175"
     CDS             complement(<1..630)
                     /locus_tag="VFY80_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6008122.1"
                     /translation="MRHILRLSMLLSMAATPLLSQQAAGPVQQRAVLVTGASSGIGRK
                     IAELLASQGHFVYAGARKPADIEELSKIRNIQGIKLDVTVASDIAAAIETVRKGGRGL
                     HGIVNNAGIAILSPLVQTDEKEMASIFDVNVHGPYRITKAFAPLLVESKGRVVTISSI
                     SGILSGGFFGPYSMTKHAVEAFGDALAVEMAPLGVRSSLIEPGNFKSEIG"
     gene            complement(793..1164)
                     /locus_tag="VFY80_09180"
     CDS             complement(793..1164)
                     /locus_tag="VFY80_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6008123.1"
                     /translation="MSTGRKATRKKSTAKRAGRVERGIIEGLNDAIAFERGDGNVGRV
                     LTARRAEAAPAPTFTKGRVIDLRHRLGISQPVFAQALNVSNDTVRGWEQGRNPPSGAA
                     ARLLELVEENPDLVLRKVSER"
     gene            1847..2377
                     /locus_tag="VFY80_09185"
     CDS             1847..2377
                     /locus_tag="VFY80_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX6008124.1"
                     /translation="MDHSKHRMGPEIVIPKGALYTKADVEFMQGMIAHHAQAIVMSRM
                     AETNGASPQLLKLSRKIDQSQIPEILIMQDWLRRHGQFAPDTASWHDMRMDGMLTDDE
                     LKALSEAKGVAFDRLFLNGMIKHHAGALEMVDDLFKTPGAGQEVDVNVFANDVVTAQT
                     AEIGIMRRLLSQLPPE"
     gene            2432..>2958
                     /locus_tag="VFY80_09190"
     CDS             2432..>2958
                     /locus_tag="VFY80_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008125.1"
                     /translation="MRGSILLLATTVVPTMVSAQTYPTNKDPRSGLKPGRYDAGIAIS
                     GMKQVSHVKKPAVFDSAAGLKFINSDLAFGTHYVYQANFAGFTIWDITDPANPVMMSA
                     TKCITSQGDPTIYENLLFLSAEGAGNRNDCGDGGVQDPKDHMAGVRIFDVSNPKAPKL
                     VKNVQTCKGSHTHTLV"
BASE COUNT          541 a          999 c          913 g          505 t
ORIGIN      
        1 gccgatctcc gacttgaagt tgcccggctc gatgaggctc gaacgcacgc cgagcggcgc
       61 catctcgacc gcgagcgcgt cgccgaatgc ctcgacggca tgcttggtca tgctgtacgg
      121 gccaaagaag ccgccggaga gaatgcccga gatggaactg atggtcacca cgcgcccctt
      181 cgattcgaca agcagcggcg caaacgcctt ggtgatgcga taggggccgt gcacgttcac
      241 gtcgaagatc gaggccatct ccttctcgtc ggtctgcacc agcggcgaga gtatcgcgat
      301 gcctgcgttg ttcacgatac cgtgcaagcc gcgtccgccc ttgcgcaccg tctcgatggc
      361 cgcggcgatg tcggacgcaa cggtgacgtc gagcttgatg ccctgaatgt tgcggatctt
      421 cgacagctcc tcgatgtcag cgggcttgcg cgcaccggcg tacacgaagt gaccctgcga
      481 tgcgagcagc tcggcgatct tgcgcccgat accgctgctc gcacccgtga caagcaccgc
      541 acgttgctgc acgggcccgg cagcctgctg cgagaggaga ggcgttgcag ccatggacag
      601 caacatggaa agccgaagga tgtggcgcat cgtgtgctcg aggctgcggg agggagtttt
      661 tgcccatcga gaagggcggt gctgggaaac agcgatcgaa agtgacgcac gcgatcggcc
      721 agcgccagcc gcagattacg agccgcagat gaccagccgc aggccgtcat cgctttgtcc
      781 cgcgccgacc gcctaacgct cggagacctt ccggagcacg aggtccgggt tctcctcgac
      841 gagctcgagg aggcgcgcgg cagcgccgct cggcgggttg cggccctgtt cccagccgcg
      901 gacggtatcg ttgctcacgt tcagcgcctg cgcgaacacc ggctgcgaga tgcccaggcg
      961 atggcgaaga tcgatgaccc gacctttcgt gaacgtcggc gccggcgccg cctcggcacg
     1021 gcgcgccgtc agcacgcgtc ccacgttccc gtcaccgcgc tcgaacgcga tcgcgtcgtt
     1081 gagtccctca atgatcccgc gctcgacgcg tcccgcgcgc ttcgcggtcg acttctttct
     1141 cgttgccttt cttccagtcg acatagaccc gccctcctat gggcgctcca gcgtgtttgc
     1201 gtgctcggag gacccgcccc gcatcacggc tcccggttga gcatctcgac cagcgcggcc
     1261 atcggccttt tgccatccgc ggagaggtta tccgcctcgt tcttcgcata ggcgtagagc
     1321 aggtagacct gacgcgcagc ttgcgcacgc ccccggtctc cgcgatcacc gccccctccg
     1381 tgggggtctc cgcaagccgc acggccagcg cgtcgaggtc ggcctcggag aacagccctt
     1441 tcgccgcgcg gtcgaacgac cggagagcga tcacggtcgg cagggcgggg gcctcgcgca
     1501 tggaagtatg gtaacggtta ctatacacaa agtcaagtaa tggctactac cttatgagcc
     1561 atccaacctc acgaaggaga tgccacgccg ccgttcggcg gaacttggcg cccggatgta
     1621 gcggcccctg cggatgagca tcaacaaccc ggaggctcgc cccgtattct ttcgtgatcg
     1681 cgggcgcctc cccttgcgcc actctccgcc ggcttgctcg attagctggc cccgtctcag
     1741 gagcaccttc ggcatgacgg cttctcgttc gataatcgcg ctcaccgttg tctgcgtcct
     1801 cgccggcgcc tcctcgcgcg ccttcgcgca gagtggaacg ccgagcatgg atcacagcaa
     1861 gcaccgcatg ggccccgaga tcgtcatccc gaagggcgcg ctctatacga aggcggacgt
     1921 cgagttcatg cagggcatga tcgcgcacca cgcccaggcc atcgtgatgt cgcggatggc
     1981 ggaaacgaat ggcgccagtc cgcagctgct caagctctcg cggaagatcg accagtcgca
     2041 gataccggag atcctcatca tgcaggactg gctccgccgg cacgggcagt tcgccccgga
     2101 cacggcctcc tggcacgaca tgcggatgga cggcatgctc accgacgacg agctaaaggc
     2161 tctcagcgag gcgaagggcg ttgccttcga ccggctcttt ctcaacggga tgatcaaaca
     2221 ccacgccggc gcgctcgaga tggtcgacga cctcttcaag accccgggag ccggccagga
     2281 agtggatgtc aatgtcttcg ccaacgatgt cgtcacggcc cagaccgccg agatcggcat
     2341 catgaggcgc ttgctttcgc agctcccccc agagtaaaac acgagtgatc gccccacgtc
     2401 cctacccctg gaacaaacgt cgatgcgtat gatgcgcggc tccatcctgc tgctggcgac
     2461 gaccgtcgtg ccgaccatgg tgtcggcaca gacgtatccg accaacaagg atccccgaag
     2521 cggtctcaag cccggccgtt acgacgcggg cattgcgatc agcggcatga agcaggtgtc
     2581 gcacgtgaag aagccggcgg tcttcgactc ggcggccggc ctgaaattca tcaactccga
     2641 cctggcgttc ggcacccatt acgtctacca agccaacttt gccggcttca cgatctggga
     2701 catcaccgac cccgccaacc cggtgatgat gtcggcgacg aagtgcatca cctcgcaggg
     2761 tgatccgacc atttacgaga acctgctctt cctgtccgct gaaggcgcgg gcaaccgcaa
     2821 tgattgcggc gacgggggcg tgcaggatcc gaaggaccac atggccggcg tgcgcatctt
     2881 cgacgtgtcc aacccgaagg cgccgaagct cgtgaagaac gtgcagacgt gcaaaggctc
     2941 gcacacgcac accctggt
//
LOCUS       DASURG010000517         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_497119, whole genome shotgun sequence.
ACCESSION   DASURG010000517 DASURG010000000
VERSION     DASURG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497119"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFY80_09195"
     CDS             <1..733
                     /locus_tag="VFY80_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6008126.1"
                     /translation="KKTLPSRAARAAEIVRPSVVRVRGLTEALPQDALKKKSKLDARK
                     SRQDAAPPAKSAPEGWDEASVGSGIVIVDDGTILTNLHVVYGAGRVMVTFHDGSESEA
                     ELLSVHPENDLAVIKVRKVPDDLPVATLGPAGRLKPGDEVVAVGFPFGIGPSVSAGVV
                     SGLNREFLSPEGKRVLTGLIQFDAAANPGSSGGPLVTMQGDVVGIVTAILNPTGSGTF
                     IGVGFAVTIGAARRAVDGTTLLPEP"
     gene            736..1737
                     /locus_tag="VFY80_09200"
     CDS             736..1737
                     /locus_tag="VFY80_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6008127.1"
                     /translation="MSDDSNVHGLMQRILYEIKRVVVGQDHFLERILVAMLAQGHLLV
                     EGVPGLAKTLTIKTLARTIRGSFKRIQFTPDLLPADLVGTRIYNQKTGDFSTALGPVF
                     TNLLLADEINRAPAKVQSALLEVMQERQVTIAGETYQVPVPFLVMATQNPIETEGTYP
                     LPEAQVDRFMMKVVVDYPTEEEEFVIVERVTGAAAAVAAVATTDQLGALQEACRKVYV
                     DPALFQYAVKLVSATRRCERYGLSEFARYITFGASPRATICLIEGARALAFLRGREYA
                     LAEDVSDLVPDVLRHRLVLSYDALSDAQSADTLIARVMQAVPAPAAPLEAHVQIAAR"
     gene            1715..2689
                     /locus_tag="VFY80_09205"
     CDS             1715..2689
                     /locus_tag="VFY80_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6008128.1"
                     /translation="MFKSRQGKLKLVRPAEHPAASAESALRHVEWTLLRRLDGILQGN
                     YLTFFRGFGIDLADLREYHFGDDARTIDWNATARLQVPHVRQFNQEREITAWFLIDLS
                     ASVDFGTGGNTKRAIAETFVALVARLLTRHGNRAGALLYTDRIHEVVLARGGRRHVVH
                     LITEMQKARSTPRAGAPTRLGELLDRALQVIKRRSVVFVVSDFISDGDWARALAVLGQ
                     RHELLAVRLYDALEREFPDLGLMIVEDAETGRQLFVDTHDAAFRRRFAELAAKRETAV
                     RQAFADAGADCIELGTGSDVVDTLLRFARLRKRRAQLASGGVPPALMR"
     gene            2686..>3036
                     /locus_tag="VFY80_09210"
     CDS             2686..>3036
                     /locus_tag="VFY80_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX6008129.1"
                     /translation="MSFLAQLRQTTMLWPEGLWLLAVLPVLIAMYVWLGRRHQRVHAR
                     LAGLWQSDRSVRSAFRRALPPFLFLLGLAALITAVGRPQAVLTLPSVHKSVMLAIDTS
                     GSMRATDVKPSRLAA"
BASE COUNT          521 a          950 c         1004 g          561 t
ORIGIN      
        1 aaaaaagacg cttccatccc gtgccgcgag agccgcagaa atcgtgcgac cgtccgtggt
       61 gcgcgtgcgc ggattgaccg aagcactccc gcaagacgcc ttgaagaaaa agtctaagct
      121 tgatgcgcgt aaatcgcgtc aggatgccgc gccgcccgcc aagagcgccc cagaagggtg
      181 ggacgaagcc tcggtcggtt caggtatcgt gatcgtggac gacggcacca tactcacgaa
      241 tctgcacgtc gtctatggcg caggtcgggt gatggtgacg tttcatgacg gctcggaatc
      301 cgaagccgag ttgctgagcg tgcatccgga aaacgacctc gcggtgatca aggtgcgcaa
      361 ggtgccggat gacctgccgg tcgccacgtt gggtccggca ggccgcttga agcccggcga
      421 cgaggtggtg gcggtcggtt ttccgttcgg catagggccg tcggtgtcgg cgggcgtggt
      481 ttcaggcctc aatcgcgaat ttctctcgcc cgaggggaaa cgcgtgctga ccgggctcat
      541 acagttcgac gccgcagcca acccgggcag ctcgggcggt ccgctggtta ccatgcaggg
      601 cgatgtggtg ggcatcgtca ccgcgatcct caacccgacg ggtagcggca cgttcatcgg
      661 cgtcgggttt gcagtcacca tcggcgcagc gcggcgcgcc gtcgacggca ccaccttact
      721 tccggagcct tgaacatgag cgacgactcg aacgtccacg gcctgatgca gcggatcctg
      781 tatgaaatca agcgcgtggt tgtcggtcag gaccactttc tcgagcgcat actggttgcg
      841 atgctggcgc agggccatct gctggtggaa ggcgtccccg gattggcgaa gacgctgacg
      901 atcaagacgc ttgcgcgcac catacgcggt tcgttcaagc gcattcagtt cacacccgac
      961 ctgcttcccg cagacctcgt cggcacgcgc atctacaacc agaagacggg agacttcagc
     1021 actgcgctcg ggcccgtgtt caccaatctc ttgctcgccg atgagatcaa tcgcgcgccg
     1081 gcgaaagtgc agagcgcgct gctggaagtc atgcaggagc gccaggtcac catcgccggc
     1141 gaaacctatc aggtgccggt gccgtttctc gtcatggcga cacagaatcc gattgaaacg
     1201 gaagggacct atccgctgcc cgaagcgcag gtcgatcggt tcatgatgaa agtggtggtg
     1261 gactacccga cggaagagga agagttcgtc atcgttgaac gggtcaccgg cgccgcggcc
     1321 gcagttgcgg cggtagcaac gacggatcag ctcggcgcat tgcaggaggc gtgccgcaag
     1381 gtctatgtcg atccggcgct gttccagtac gccgtcaagc tcgtatccgc cacgcggcgc
     1441 tgcgagcgtt acggtctcag cgaattcgcc cgctatatca cgttcggtgc gagcccgcgc
     1501 gcgacgatct gcctgatcga aggcgcccgc gcgctcgcat tcctgcgcgg acgcgagtat
     1561 gcgttggcgg aagacgtgtc agacctcgtc cccgatgtgt tgcgccaccg gctcgtgctt
     1621 tcctacgacg cgctttccga cgcgcagtcg gcggatacgc tcattgcccg cgtgatgcag
     1681 gccgtgcccg cacctgccgc gccgctcgag gcccatgttc aaatcgcggc aaggtaagct
     1741 caagctcgtc cggccggctg agcacccggc ggcgagcgca gaaagcgcgc tccgccacgt
     1801 cgagtggacg ttgctgcgcc ggctcgacgg gatactgcag ggcaactatc tcaccttttt
     1861 tcgcggcttc ggcatcgatc tcgcggactt gcgcgagtat cacttcggtg acgatgcgag
     1921 aacgatcgac tggaatgcga cggctcgcct gcaagtgccg catgtacgcc aattcaacca
     1981 ggagcgggag atcacggcgt ggttcctcat cgatctcagc gcctcggtgg atttcggtac
     2041 gggcggcaac accaagcgcg ccatagcgga aaccttcgtg gcgctggttg cgcggcttct
     2101 cacccgtcac ggcaatcggg ccggcgcgct gctctacacc gaccgcatcc atgaggtcgt
     2161 gctcgcgcgg ggcggacgtc gccacgtcgt tcatctcatc actgaaatgc agaaagcccg
     2221 atcgacaccg cgcgcgggtg cccccacgcg cctgggcgaa ctcctggatc gtgcgctgca
     2281 ggtcatcaag cgtcgctcgg tcgtgttcgt ggtgtcggac ttcatcagcg atggcgattg
     2341 ggcacgcgca ctcgcggtgc tggggcaacg ccatgagctg ctcgcagtgc ggctctatga
     2401 cgcgctggag agggaatttc cggatctcgg gttgatgatc gtggaggatg ccgagaccgg
     2461 ccggcaattg ttcgtggaca cgcacgatgc ggcatttcgc cggcgctttg cggaactcgc
     2521 ggcgaagcgt gaaacggccg tgcgccaggc gttcgcggac gccggtgccg actgcatcga
     2581 gctcgggacc ggttccgatg tcgtcgatac gctcctgcgc tttgctcggc tgcgcaagcg
     2641 ccgggcgcag ctcgcgagcg gcggcgtacc gccggcactg atgagatgag tttcctcgca
     2701 cagctgcggc aaacgacgat gctgtggccg gaagggctct ggctcctcgc tgtattgccc
     2761 gtgttgatcg cgatgtatgt atggttgggc aggagacacc agcgcgtgca cgcgcgcctt
     2821 gcggggctct ggcaatcgga ccgctcggtg cggagtgcat tccgtcgcgc gcttccgccg
     2881 tttctttttc tgctgggtct cgccgcactg atcacggcgg tgggccggcc gcaggcggtc
     2941 ctcacgctgc cctctgtgca caagagcgtg atgctcgcga tcgatacgtc gggcagcatg
     3001 cgcgcgaccg atgtcaagcc cagccgcctg gccgcc
//
LOCUS       DASURG010000518         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_294390, whole genome shotgun sequence.
ACCESSION   DASURG010000518 DASURG010000000
VERSION     DASURG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294390"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFY80_09215"
     CDS             <1..647
                     /locus_tag="VFY80_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX6008130.1"
                     /translation="HFGATLVCGFAHIEGMPVGIIANNGILFSESAQKGAHFIELCCQ
                     RKIPLVFLQNITGFMVGRKYENEGIARHGAKMVTAVSCAQVPKFTVIIGGSFGAGNYG
                     MCGRAFGPRFLWTWPNARIAVMGGEQAASVLAQVRRESLQARGLAWSEEEESAFKAPI
                     LEQYARQSSAWYASARLWDDGVLDPVATRTTIAHGLAASAHAPIERTAFGVLRM"
     gene            681..1493
                     /locus_tag="VFY80_09220"
     CDS             681..1493
                     /locus_tag="VFY80_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6008131.1"
                     /translation="MTENVLLHIDDRGTATVTLNRPEVHNAFDPKLVKTLTSILQRLE
                     GDSEVRAVVLRGAGRSFCAGADIEEMRKSATYAPEQNFEIARQSAQMFHALYALGKPT
                     IACVHGAARGGGCGLIAACDIAIAARLATFRFSEVKIGVIPAMISPYVIAAIGERMAH
                     RYMLTGEQFDADEAWRIGLVHEVVEADALEAKLTDLLAQLRTSGPNAVREIKRLIPVS
                     AHAAIESGIIEETARRIAAIRATPEAQEGLSAFLEKRKPAWGARDRAQGPKS"
     gene            1490..2401
                     /locus_tag="VFY80_09225"
     CDS             1490..2401
                     /locus_tag="VFY80_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX6008132.1"
                     /translation="MKLPSRVKIVEVGPRDGLQNEPTPVSAATKIELIHRLQDAGIRV
                     IEATAFVSPKWVPQMVDNAEVMAGIRRRPDVSYPVLVPNLKGLEAAINAGAHEVVVFG
                     AATESFSRRNTNCSIAEGLARFSEVCKTALAAGLKVRGDISVCLGCPYEGAVSPEAVV
                     RVARELDAMGCYEITIADTIGVGTAGQTRAAFEAVMHYIAPERLAGHFHDTYGQALTN
                     IYAALECGVATYDSSVAGLGGCPYAKGATGNVATEDVVYLLNGLGIETGIDLEKVIAA
                     GEYICGILERPTHSRAARALLAKRHAA"
     gene            2434..2985
                     /locus_tag="VFY80_09230"
     CDS             2434..2985
                     /locus_tag="VFY80_09230"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEX6008133.1"
                     /translation="MPLSSSAPRKLMHNRTIECRGYERDDGLWDIEAHLVDTKTYLHT
                     RRQGGTERRPGEPVHDMWLRLTIDLDMKVHDAEAVTDVGPYPHCGHIAPNFKSLIGLT
                     IGPGWRRRTFELLGGTRGCTHLVELLGPLGTTAFQATGRAREGKHAASSVMRRPYQLN
                     ACHVYQDDSEAVRERWPQFYTGK"
     gene            complement(3007..3387)
                     /locus_tag="VFY80_09235"
     CDS             complement(3007..3387)
                     /locus_tag="VFY80_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008134.1"
                     /translation="MVDLAEGIKRVGFKRWYERQLIESHVYFVTAFLSLIVIVAGLEG
                     YNAAAEGSTRLYMLALIAGGIVLCAWAFRRYQFMLGRAVRAAERSICSGCATYGTFEV
                     VPQAQAVRALTVRCRKCGHEWRME"
     gene            complement(3393..>3843)
                     /locus_tag="VFY80_09240"
     CDS             complement(3393..>3843)
                     /locus_tag="VFY80_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008135.1"
                     /translation="LLGPVMSVSGNEIAIRFGRTRVIFTFMTASGLTACVLGYTAALP
                     WAIVFLLMSIHYGLMLGDSAALTSGAVASAHPDERGATMAVYSFSGFSVAFLAPLVFG
                     SVLDLAGGNRSLVAWGLAFASIGIFGALAPLARWAYLRRGRVAPPTG"
BASE COUNT          756 a         1211 c         1257 g          619 t
ORIGIN      
        1 cgcacttcgg cgccacgctg gtgtgcggct tcgcgcacat cgagggcatg ccggtgggca
       61 tcatcgccaa caacggcatc ctgttttccg agtcggccca gaagggcgcg cacttcatcg
      121 agctgtgctg ccagcgcaag attccgctgg tgttcctgca gaacatcacc ggcttcatgg
      181 tcggccgcaa gtacgagaac gagggcatcg cccgccacgg cgcgaagatg gtgaccgcgg
      241 tgtcgtgcgc ccaggtgccc aagttcacgg tgatcatcgg cggcagcttc ggcgccggca
      301 attacggcat gtgcgggcgg gcgttcgggc cgcgcttcct gtggacctgg cccaatgcgc
      361 gcatcgcagt catgggaggc gagcaagcgg cgagcgtgct ggcgcaggtg aggcgcgaaa
      421 gcctccaggc gcgcggtctt gcgtggagcg aggaggaaga aagcgcattc aaggcgccga
      481 tcctcgagca gtacgcgagg cagtcgagcg cgtggtatgc gagcgcgcga ctctgggacg
      541 acggcgtcct cgatccggtg gcaacgcgca ccacgattgc acatgggctc gcggcaagcg
      601 ctcatgcgcc gatcgagagg accgcgttcg gggttttaag aatgtaggac ttataattgt
      661 ccaacccacc cgcagccaac atgaccgaaa acgtcctgct tcacatcgac gatcgcggga
      721 ccgcgaccgt aacgctgaac cgtccggaag tgcacaacgc cttcgacccg aagctcgtga
      781 agacgctcac ttcgattctg cagcggcttg aaggtgactc cgaagtgcga gcggtagtgc
      841 tgcgcggtgc ggggaggagc ttctgtgccg gcgccgacat cgaggagatg cgcaagagcg
      901 cgacctacgc gcccgagcag aacttcgaga tcgcgcgtca gtcggcgcag atgttccacg
      961 cactgtacgc actcggcaag ccgacgatcg cctgcgtgca cggcgcagcg cgcggcggcg
     1021 gatgcgggct gatcgcagcc tgcgatatcg cgatcgccgc gcgccttgcc actttccgct
     1081 tctccgaggt gaagatcggt gtcattccgg cgatgatcag cccgtacgtc attgctgcga
     1141 tcggcgagcg catggcgcat cgctacatgc tgaccggcga gcagttcgat gcggatgaag
     1201 cgtggcgcat tgggcttgtc cacgaagtcg tagaggccga tgcgctggaa gcgaaactga
     1261 cggatctgct cgcccagctt cgaacgagcg ggccgaacgc ggtgcgggaa atcaagaggc
     1321 tcattcccgt gagcgcacac gctgcgatcg aaagcggcat catcgaggaa acggcgcggc
     1381 gcatcgccgc gatccgcgcg acgccggaag ctcaggaagg cttgtcggcc tttctcgaaa
     1441 agcgcaagcc ggcatggggc gcgcgcgatc gagcgcaagg gccaaaatca tgaagctgcc
     1501 gagccgggtc aagatcgtgg aggtcggccc gcgcgacggc ctgcagaacg agcccacccc
     1561 tgtctccgcc gcgaccaaaa tcgaactcat ccaccgattg caggatgcag ggatacgggt
     1621 gatcgaggcg accgcgttcg tctcgccgaa gtgggtaccc cagatggtgg acaacgccga
     1681 agtcatggcg gggatccgcc gcaggccgga cgtttcttat ccggtgctcg tgcccaacct
     1741 caaaggcctc gaggctgcga tcaacgccgg cgcgcacgaa gtcgttgtat tcggcgcagc
     1801 aaccgaatcg ttttcccgcc gcaataccaa ctgttccatc gcagaggggc tcgcgcgatt
     1861 ttcggaagta tgcaaaacgg cccttgccgc ggggctcaag gtgcgaggcg acatctccgt
     1921 ttgcctcggg tgcccttacg agggcgcggt cagccccgaa gcggtggtgc gtgtggcgcg
     1981 ggaactcgat gcgatgggtt gttacgaaat caccatcgcc gacaccatcg gcgtcggcac
     2041 agcgggacag acgcgcgcgg cgttcgaggc ggtgatgcac tacatcgctc cggagcgcct
     2101 cgccggtcac ttccacgata cctatggcca ggcgctcacc aatatctatg cggcgctcga
     2161 gtgtggtgtc gcgacctacg acagctcggt ggcgggcctc ggcggatgcc cgtatgcgaa
     2221 aggcgcgacc ggcaacgttg caaccgagga cgtggtgtac ctgttgaacg ggctcggcat
     2281 cgagacgggt atcgatctcg agaaagtcat cgccgccggc gagtatatct gcggcatact
     2341 ggaacgcccc acgcattcgc gtgcggctcg cgcactcctg gcgaagcgcc acgccgcctg
     2401 attttcgaac gtcgatgtga ttccggagtt tgtatgccgt tgagttcgag cgcacctcgc
     2461 aagctgatgc acaaccgcac gatcgaatgc cgtggttacg agcgcgacga cggcttatgg
     2521 gacatcgagg cgcatctcgt cgacacgaag acctacttgc acacgcgccg gcaaggggga
     2581 acggagcgtc ggcccggcga gccggtgcac gatatgtggc tgcggctcac catcgatctc
     2641 gacatgaagg tccacgatgc cgaagcggtg accgatgtcg ggccctatcc gcactgcggc
     2701 catatcgcgc ccaatttcaa gtctctgatc gggcttacca tcggtcccgg ctggcgccgc
     2761 aggacgttcg agcttcttgg cggcacgcgc ggctgcacgc atctcgtgga attgctcggg
     2821 ccgctgggta ccaccgcgtt tcaggcgacg gggcgtgcac gagaagggaa gcatgccgcc
     2881 tcttccgtca tgcgcagacc ctaccagctc aacgcctgcc acgtgtacca ggacgattcc
     2941 gaagcggtgc gggagcgctg gccgcagttc tatactggaa agtagaagag gcagctgggc
     3001 gaccgctcac tccatccgcc attcatgccc gcatttgcgg caacggacgg tgagcgcgcg
     3061 gaccgcctgg gcttgcggca cgacttcgaa ggtgccgtaa gtcgcgcatc cgctgcagat
     3121 ggaacgctcg gccgcccgca ccgcgcggcc cagcatgaac tgatagcggc gaaatgccca
     3181 ggcacagagc acaatcccgc cggcgatgag ggccagcatg taaagccgcg tcgaaccctc
     3241 tgcggccgca ttgtagcctt cgagccccgc cacgatgacg atgaggctca ggaacgcggt
     3301 gacgaaatac acgtggcttt caatgagctg gcgctcatac cagcgcttga accccacacg
     3361 cttgatgcct tcggcgagat cgaccatatg cgttaccctg ttggaggcgc aacccgcccg
     3421 cggcgcagat acgcccacct cgccaacggc gccagcgcgc cgaatattcc aatactggca
     3481 aacgccaatc cccaggcgac gaggctgcga ttgccgccgg caaggtcgag caccgaacca
     3541 aatacgagcg gggccagaaa agcgacactg aatccggaaa acgagtagac cgccatcgtc
     3601 gcgccgcgtt catcgggatg cgccgacgct acggcaccgg aggtaagcgc cgcggagtcc
     3661 ccgagcatga gtccgtagtg gatgctcatc aggaggaaca cgatcgccca cggcaacgcc
     3721 gccgtgtatc caagcacgca cgccgtcaga ccggacgcgg tcatgaaggt gaagatcacg
     3781 cgcgtacgcc cgaagcggat cgcgatctcg ttgccggaga cgctcatcac gggaccgagg
     3841 aga
//
LOCUS       DASURG010000519         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_803196, whole genome shotgun sequence.
ACCESSION   DASURG010000519 DASURG010000000
VERSION     DASURG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803196"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFY80_09245"
     CDS             <1..993
                     /locus_tag="VFY80_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX6008136.1"
                     /translation="AVSREAGMVIGASVVLVYTLFGGMWSVAVTTFVQMIVIVIGLFY
                     IAWLVAGMAGGASSVIEHAAAQGKFEFLPRLSLADIVAFVAALITMGFGSVPQQDVFQ
                     RVNASRTEATAAWGSILGGCAYFLFAFVPLFLAYAATVIDPTMVAQLMEKDSQLILPN
                     LILGHLPLFAQIVFFGALLSVIMSTASGTLLAPSVTISENVLKGIFRAMSDRAFLWMN
                     RAVVVCFAILVTVYAVFTDETIHKMVENAYKVTLVSAFVPLVCGLYWKRATTQGAAFA
                     IVGGLGSWLALELVASDALWPPQFVGFLIAIAGMLLGSLAPQRYGVSRAGVQSA"
     gene            993..1610
                     /locus_tag="VFY80_09250"
     CDS             993..1610
                     /locus_tag="VFY80_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008137.1"
                     /translation="MHHDSTRAYSAPSAGRAVSAYAWFKLCVIALLACNAVLFVLAGT
                     ANEALDSAAWFALLVLFELESAFALRFRHAHATAVIRALRLAAALAIGLAAVGYLRDR
                     EWLDATNAWLWIAVVALLELEVRRPRSAAAHRPAFVLTAAALYGGLAAVAAAWAWRGE
                     WFDAYDALLWLVAFVTLELDVLGRAKAAARVQPSGESTAEFGDKV"
     gene            complement(1572..2525)
                     /locus_tag="VFY80_09255"
     CDS             complement(1572..2525)
                     /locus_tag="VFY80_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX6008138.1"
                     /translation="MQFYDIFNGDADGICALQQLRIEDPRPSVLVTGAKREIDLVARV
                     TPGAGDELTVLDLSFKVNARAVADALDCGARCRYFDHHAAGDVPQHPNLRTFIDTAPD
                     VCTSLIVDRYLSGRQRIWAVVAAFGDNLIKSALRTAELLELEGREVQLLRELGEALNY
                     NAYGDTVDDLHYHPADLFETLSHYRDPRDFVEAEPIFDVLRNAWQDDLARARNVAPER
                     ETAGGALYVLPDAAWSRRISGLFANQLAQEAPARAHAVLVKKPEGYSVSVRAALEKPV
                     GAEALCAKYETGGGRKAAAGINHLPEGSLSDFITEFSRAFT"
BASE COUNT          385 a          823 c          820 g          519 t
ORIGIN      
        1 gctgtctcgc gcgaggcagg catggtgatc ggcgcgagcg tcgtgctggt ctatacgctt
       61 ttcggcggca tgtggtcggt tgccgtcacc acgtttgtgc agatgatcgt catcgtcatc
      121 ggcctcttct acatcgcctg gctggtggcc gggatggcgg gaggcgcctc ctccgtcatc
      181 gagcacgccg ccgcgcaggg caagttcgaa tttctgccgc gactctccct cgccgacatc
      241 gtcgcgttcg tggcggccct catcacgatg ggattcggct cggtgccgca gcaggacgtg
      301 tttcagcgcg tgaacgcatc gcgcaccgag gcgacggcgg cgtgggggtc gatcctcggt
      361 ggatgcgcct attttctctt tgcctttgtc ccgctcttcc tcgcctacgc cgctaccgtc
      421 atcgatccga cgatggtggc gcagctgatg gaaaaggact cgcagttgat tctccccaac
      481 ctgatccttg ggcatctgcc gctgttcgcg caaatcgtct tcttcggtgc gctgctgtcg
      541 gtcatcatga gcactgcgag cggcacgctg ctcgcaccgt ccgtgaccat atccgaaaac
      601 gtgctcaagg gcatttttcg cgcgatgagc gacagggctt tcctgtggat gaaccgtgca
      661 gtcgtcgtat gctttgcgat cctcgtgacc gtgtatgcgg tcttcaccga cgagacgatc
      721 cataagatgg tggagaacgc gtacaaggtc accctcgttt ccgcgttcgt gccgctggta
      781 tgcgggctct actggaaacg ggcgacgacc cagggtgcgg cttttgcgat cgtcggcgga
      841 ctcgggagct ggctcgcgct cgagcttgtc gcaagcgatg ccctgtggcc gccgcagttc
      901 gtcggattcc tgattgcgat tgcaggcatg ctcctcgggt ccctggcccc gcagcgctac
      961 ggtgtttccc gcgcgggcgt gcagtccgca tagtgcatca cgactcgacc cgcgcatata
     1021 gtgcgccgag cgcagggcgc gcggtctccg cttacgcgtg gttcaagctg tgcgtcattg
     1081 cgctccttgc gtgcaacgcc gtgctgttcg tgctcgcggg cacggcgaac gaggcgctcg
     1141 actccgccgc ctggttcgcg ttgctcgtgc tcttcgagct ggagagcgct ttcgcgctgc
     1201 gctttcgcca tgcccatgcc acagcggtga tacgcgcctt gcggctcgcc gccgcgctcg
     1261 ccatcggcct tgctgcagtt gggtatcttc gagaccggga gtggctcgac gccaccaacg
     1321 cctggctctg gatcgcggtc gtcgcattac tcgaactcga ggttcggcgt ccgcgcagtg
     1381 ccgcggcgca tcggccggca ttcgtgctga cggctgccgc cctctacggc ggcctcgccg
     1441 cggtagcggc agcatgggcg tggcgcggcg agtggttcga tgcttacgac gcgctgctgt
     1501 ggctcgttgc gttcgtcacg ctggagctcg acgtgctcgg ccgcgcgaag gctgcagcgc
     1561 gcgtgcaacc gtcaggtgaa agcacggctg aattcggtga taaagtctga caagctgccc
     1621 tccggcagat gattgatgcc ggcggccgct ttccttccac cgcccgtttc atacttcgcg
     1681 cacagcgctt cggcgcccac aggtttctcg agcgccgcgc ggacgctcac ggaatagcct
     1741 tccggcttct tcaccagaac ggcgtgcgcg cgcgccggcg cctcctgcgc gagctgattc
     1801 gcaaaaagcc cgctgatgcg gcggctccag gcggcatcgg gcagaacata gagcgcgcca
     1861 ccggctgttt cgcgctccgg cgcgacgttt ctcgcgcgtg cgagatcgtc ctgccaggcg
     1921 ttcctgagga cgtcaaaaat gggttccgct tcgacgaaat cgcgcgggtc gcgatagtgg
     1981 ctcaacgtct cgaaaaggtc cgcgggatga tagtgaagat catccacggt gtccccatag
     2041 gcgttgtaat tgagcgcttc gcccagctcg cgcagcagct gcacctccct tccctcgagc
     2101 tcaagcagct cggcagtccg cagcgccgat ttgatcaggt tgtcgccgaa agccgcgacc
     2161 accgcccaga tcctctgccg tcccgaaaga tagcggtcca cgatgaggct cgtgcagaca
     2221 tcgggcgccg tgtcgataaa cgttcgcaag ttgggatgct ggggaacgtc cccggcagca
     2281 tgatgatcga agtagcggca ccgcgcgccg caatcgagcg cgtccgccac cgcgcgcgcg
     2341 ttgaccttga atgagagatc gagcacggtg agttcgtcac ccgcgcccgg tgtgacgcgg
     2401 gcgacgaggt cgatctcgcg cttcgccccg gtgacgagaa cgctggggcg cggatcctcg
     2461 atccgcagct gctgaagcgc gcagatcccg tccgcgtcgc cattgaagat gtcatagaat
     2521 tgcatcagga aatccggtca gccacgg
//
LOCUS       DASURG010000520         4491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_88738, whole genome shotgun sequence.
ACCESSION   DASURG010000520 DASURG010000000
VERSION     DASURG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88738"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFY80_09260"
     CDS             <1..588
                     /locus_tag="VFY80_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6008139.1"
                     /translation="IDGKRRTPAGAELRWRMAVLGRANALPIFFIEHLTSMAERRRAH
                     VTTAVGSSGSATPSSPSTHPNGVYALERTYIVTPDVEASAALYATVLGMPKPAVTKGT
                     VIMSNMAVFQIGPTGLGVVQPYAEGPAAEALERRGPGPFQALYRTRSMGAAARWMQAQ
                     GLPPLQRGVRNTGEVAMLATPAEACGAYVGFVGPE"
     gene            complement(589..1512)
                     /locus_tag="VFY80_09265"
     CDS             complement(589..1512)
                     /locus_tag="VFY80_09265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX6008140.1"
                     /translation="MKAAFFMKHGGPEVMECGDVPDPAAGPGQVLVDVHAASVNGADW
                     KVRAGKYAPITAFPYIPGRDFSGVVAALGEGVKDFAIGDPVFGVCDVGQEAAYAEKVA
                     IRSSIIARKPENLSHVECAALALVGLTALSSIEDTLKLKSGEKILIQGGAGGVASLAI
                     QVAKHIGAHVISTASAANLDYVRSLGADEVIDYRARDFAESVSGCDAVFDTVGGAVAI
                     RSFAVLKPGGRAAFIASGNTAPPSPRSDVVSLRPKVGRDRPHLERIVALVARGAVRVP
                     EITVYTLSQAAEAHRISESRHLRGKLVFKVR"
     gene            1651..1944
                     /locus_tag="VFY80_09270"
     CDS             1651..1944
                     /locus_tag="VFY80_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX6008141.1"
                     /translation="MAKGYWINMFRSVKDPQKVEAYRNLAGPVMQAAGARFLVRGNPA
                     KTYEAGVMERVVVIEFESVQRAMEVHDSPGYQAALRALGDGAERDLRIVEGLE"
     gene            complement(2037..2438)
                     /locus_tag="VFY80_09275"
     CDS             complement(2037..2438)
                     /locus_tag="VFY80_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6008142.1"
                     /translation="MPETSLAFDHVHLVAKNPRTTAEWYVDKLGGKIVRSLEVKGAPQ
                     VYVSLGGFLVIVRGRRPSESANDKPGLQWGVDHFGVRVKGDFDRFCHNLRQRGVTFSL
                     EPTDFNPTTRIAFINAPDGVSVELLNRKDEL"
     gene            2497..2886
                     /locus_tag="VFY80_09280"
     CDS             2497..2886
                     /locus_tag="VFY80_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6008143.1"
                     /translation="MAKLRHIAMVVEQMEKTAQFYEQSFGMKRVRQTATAIGLSDGVV
                     SLVIIHPSNVNMKGDSRRGLHHIGFLVDDMQKEAAKVESNGAVLHGGIIHSDTGTETE
                     RKYRDPNGQIFDLTSPEYARDYWQIAV"
     gene            complement(3018..4187)
                     /locus_tag="VFY80_09285"
     CDS             complement(3018..4187)
                     /locus_tag="VFY80_09285"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6008144.1"
                     /translation="MTTVAATIPIHGTCDPRFVGVREEFERNFSERGEIGAAVCVYKD
                     GRKVVDLWGGHKDIERTIPWAEDTIVIMNSVAKSMCALCMHMLIDRGRVEFDAPVAHY
                     WPEFGQAGKARVLIRHVLSHTCGVIFCDHAPPGSWFDWDVHIRALEKQAPAWEPGTQG
                     AYNSINIGYILGEVVRRVTGKTVGTFLREEVTSRLGADYNIGLRPDEIARVSDMHRNP
                     KNTFFKVASDPATPLGRAFRSAPKTGYFQNCREIREREVASFGGHGNARAMARIYAML
                     AGNGEIDGVRLLSPQAVERAAQLVWEDDCIMTQRRIRMGYGFMHNETNTVPMGENMKA
                     FGHTGTGGAFTWCDRERNLAFAYCTNFQREGPGIGPRGAALGVAAGGGQRPDWLK"
     gene            4301..>4491
                     /locus_tag="VFY80_09290"
     CDS             4301..>4491
                     /locus_tag="VFY80_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008145.1"
                     /translation="MTTSPRAAQIRAGLSHPIIDGDGHWLEPIPVFLEYLDEVGGSKA
                     VDALRAYWQRNTAWYRADW"
BASE COUNT          804 a         1419 c         1450 g          818 t
ORIGIN      
        1 atcgatggca agcgccgcac gcccgcgggg gccgaattgc gctggcgaat ggcggtgctg
       61 ggacgagcga acgcgctgcc cattttcttc atcgagcatt tgacgtcgat ggcggagcgg
      121 aggcgcgcgc atgtcactac ggctgtgggg tcgtcgggca gcgctacgcc atcgtcgcct
      181 tcaacgcatc ccaacggcgt ctacgcgctc gagcgtacgt atatcgtgac gccggatgtc
      241 gaggcctcgg ccgcgctcta tgcgacggtg ctcggcatgc cgaagccggc cgtcaccaaa
      301 ggcacggtga tcatgtccaa catggcggtg ttccagattg gcccgacggg gcttggtgtc
      361 gtgcagccgt atgctgaggg accggctgca gaggcgctcg aacgccgcgg ccccggaccg
      421 ttccaggcgc tctatcgcac gcgaagcatg ggcgcagccg cgcgctggat gcaggcgcag
      481 ggattgcccc cgctgcagcg cggcgttcgg aataccgggg aggtggcgat gctcgccacg
      541 ccggccgagg cgtgcggcgc ctacgtgggc ttcgtcggtc ccgaatagtc agcgcacctt
      601 gaaaacgagc ttgccgcgca ggtggcggct ttcgctgatg cggtgcgctt ccgccgcctg
      661 ggacaaggta tagaccgtga tctccggcac gcgcaccgcg ccgcgtgcga caagcgccac
      721 gatgcgctcg agatgcgggc gatcgcgccc cactttgggc cgcagcgaaa cgacatcgct
      781 ccgcggcgaa gggggcgcgg tattgcccga cgcgatgaat gccgcgcgcc cgcctggctt
      841 gagtacggca aaggagcgga tcgcgacggc gccgcctacc gtatcgaaca ccgcatcgca
      901 gcccgacacc gattccgcaa agtcccgggc gcgatagtcg atcacctcgt cggcgccaag
      961 gctgcgcacg taatccaggt tcgcggcgct tgcggttgaa atgacgtgcg cgccgatgtg
     1021 cttggccacc tggatcgcca agctcgcgac gccgccggcg ccgccctgga tcaggatctt
     1081 ttcgccggac ttgagcttga gggtgtcctc gatcgaggag agtgcggtca ggccgacgag
     1141 cgcgagcgcc gcgcattcga cgtgcgaaag gttttccggt tttctggcaa tgatggacga
     1201 gcgaatggca accttctcgg cgtaagccgc ttcctgtccg acgtcgcaca ctccgaagac
     1261 gggatcgcca atggcgaaat ccttcacgcc ctcgccgagc gcggccacca cacccgaaaa
     1321 atcgcgcccc ggtatgtagg gaaatgcggt gatcggcgcg tatttgccgg cgcgcacttt
     1381 ccaatccgcg ccatttacgc tcgcggcatg aacatcgacg aggacctggc ccgggccggc
     1441 tgccggatcc ggcacgtcgc cgcactccat gacttcaggt ccaccgtgct tcatgaaaaa
     1501 cgctgctttc atcgttcgcc ccttctgcca gtgatcgatg cccccaattt tagacgctcg
     1561 ccggtgcgaa cccgatacga tttgtcggac tggaggattc gcgcgatact cgtgcgccca
     1621 accaaccaac tgtccatcgg aggagcagtc atggccaagg gttactggat caacatgttt
     1681 cgctcggtca aggacccgca gaaggtcgag gcgtatcgca atctcgccgg cccggtaatg
     1741 caggcggccg gcgcccggtt cctggtgcgc ggaaatccgg cgaagaccta tgaagccggg
     1801 gtgatggagc gggtcgtggt gatcgagttt gaaagcgtgc agcgcgcgat ggaagtgcac
     1861 gacagccccg gttaccaggc ggcgctcagg gcgctgggcg acggggcgga gcgcgatctc
     1921 aggatcgtcg aagggctgga gtagccacat ccttccggcg aacgccggac ccagccgtta
     1981 ttagaatccg gttctcaccg gactgatcgg cgtgctgtcg aatgaccgag cgtgggttac
     2041 agctcgtctt tgcggttcag aagctcgacg ctcacgccgt ccggagcgtt gatgaacgcg
     2101 atgcgcgtcg tcggattgaa atcggtcggc tcgaggctga aggtgacgcc gcgctgccgc
     2161 aggttatggc agaagcggtc gaaatcaccc ttcacccgca cgccgaagtg atcgacgccc
     2221 cactgcagcc cgggcttgtc attcgccgac tcggacgggc gccggccgcg gacgatgacg
     2281 aggaagccgc cgagcgacac atagacctgg ggtgcgccct tgacctcgag gctgcgcacg
     2341 atcttgccgc cgagcttgtc cacgtaccac tcagcggtgg tccgggggtt cttcgcgacg
     2401 aggtggacgt ggtcgaaagc aagactggtc tctggcatga tgatcgtctc ttgatggcgt
     2461 ttgggtccgg ttccagtcta accaaaggag gaattcatgg caaagctgcg ccatatcgcg
     2521 atggtggtgg agcagatgga gaagaccgcg cagttctacg agcagtcctt cggcatgaag
     2581 cgcgtgcgac agaccgccac agccatcggc ctctcggacg gcgtcgtgag cttggtcatc
     2641 atccatccct cgaatgtcaa catgaaaggc gacagccgcc ggggcttgca tcacatcggc
     2701 tttctcgttg acgacatgca aaaggaagcg gcgaaggtgg aatcaaacgg ggctgttttg
     2761 catggaggaa tcattcattc cgacaccggc acggaaacgg agcgcaagta tcgcgacccg
     2821 aacggacaga tcttcgatct cacgagcccc gaatacgccc gcgattactg gcagatcgct
     2881 gtctgattcg ggctgtcatt ccgttcgccg ttccggtgca tcgaactcgt cattccggca
     2941 catcgacctg gtcattccgg cgaaagccgg aatccaggcc agacaccggc cggtgtgacg
     3001 cggcggtcga ccgcgcgtca cttgagccag tcgggccgct ggccgcctcc agccgctacg
     3061 ccgagcgcag caccgcgcgg accaatgccc gggccttcgc gctggaagtt ggtgcaatag
     3121 gcaaaggcca ggttgcgctc gcgatcgcac cacgtgaatg ccccaccggt gccggtgtgt
     3181 ccgaacgcct tcatgttctc gcccatcggc acggtattgg tttcgttatg catgaagccg
     3241 tagcccatgc ggatgcgtcg ctgggtcatg atgcagtcgt cctcccacac gagctgcgcg
     3301 gcacgctcga ccgcctgtgg gctcagcagg cgaacgccgt cgatctctcc gttgcctgcg
     3361 agcatggcat agatgcgcgc catcgctcgc gcatttccgt ggccgccgaa cgacgccact
     3421 tcgcgctcgc ggatttcgcg gcagttctgg aaataaccgg tcttcggcgc cgagcggaac
     3481 gcgcgaccca gcggcgtagc ggggtccgag gcaaccttga agaacgtgtt cttcggattg
     3541 cggtgcatgt ccgagacgcg tgcgatttcg tcaggtctca acccgatgtt gtagtcggcg
     3601 cccagtctag aagtcacttc ctcgcgcagg aacgtgccca cggtttttcc ggtgacgcgg
     3661 cgcacgactt cgcccaggat gtagccgatg ttgatcgagt tgtaggcgcc ctgggttccg
     3721 ggctcccatg ccggcgcctg cttctcgagc gcccggatat ggacgtccca gtcgaaccag
     3781 gagccgggcg gcgcgtggtc gcagaagatg accccgcagg tgtgcgaaag gacgtgccgt
     3841 atcagcacgc gcgccttccc cgcctggccg aactcgggcc agtagtgcgc aaccggtgcg
     3901 tcgaattcga cgcgtccgcg gtcgataagc atgtgcatgc acagcgcgca catgctcttc
     3961 gcaaccgaat tcatgatgac gatggtgtct tccgcccagg ggatcgtgcg ctcgatatcc
     4021 ttgtgcccgc cccataggtc gacgactttg cggccgtcct tgtagacgca aaccgcggcg
     4081 ccgatctcgc cacgctcgct gaaattacgc tcgaattcct cgcgcacccc gacaaagcgc
     4141 ggatcgcagg ttccatggat gggtatcgtc gcggcgactg tcgtcatggc actttcttca
     4201 gtgaagtgga tgtggcggtc attctgctac agatagctgc cgttcggcca gcgcggcata
     4261 atagcgccga tcttcaatcg cgtccccaca ggaggcgaac gtgaccacga gccctcgcgc
     4321 cgcgcagatc cgcgccggac tgtcccatcc catcatcgac ggcgatggtc actggctgga
     4381 gccgattcct gtttttctcg agtatctcga cgaagtcggt ggatcgaaag cggtcgatgc
     4441 actgcgcgcc tactggcagc gcaacaccgc gtggtaccgg gccgactgga a
//
LOCUS       DASURG010000521         4974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_88875, whole genome shotgun sequence.
ACCESSION   DASURG010000521 DASURG010000000
VERSION     DASURG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88875"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFY80_09295"
     CDS             <1..1082
                     /locus_tag="VFY80_09295"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6008146.1"
                     /translation="FYVFFEGMLIPMFIIIGVWGGPNRVYAAVKFFLYTLLGSFLMLV
                     ALIYLYNAAQGSFSLLDYYELPLSLAEQVLIFIAFFMAFAVKVPMWPVHTWLPDAHVE
                     APTGGSVVLAAIMLKLGGYGFLRLSLPILPDASQYLAGFVIALSLIAVVYIGLVALVQ
                     SDLKKLIAYSSISHMGFVTLGLFIFNPHGMEGAVVQMISHGFVSGALFLCVGVLYDRL
                     HSRQIADYGGVVNTMPVFAAFFMLFAMANSGLPGTSGFVGEFLVIMGAVQVNFWYAFA
                     AASTLIFGAAYTLWMYKRVIFGAVANARVAALTDISARECLVLGLLAAAVLAMGIYPE
                     PFARVLHTSVNDLIFHATQTKLPAP"
     gene            1095..2543
                     /gene="nuoN"
                     /locus_tag="VFY80_09300"
     CDS             1095..2543
                     /gene="nuoN"
                     /locus_tag="VFY80_09300"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX6008147.1"
                     /translation="MQYILPLWPAYPELFLLVMVCGILLADLILGDDRRVVTYALTQL
                     ALAGAIAISWWTVRIEPAYTFSGMFVDDVMSDVLKLLVYLGVMGILVYSRAYLSARGM
                     FRGEFFVLTLFATLGMMIMISANHLLILYLGLELMTLSLYAMVALQRDSATATEAAMK
                     YFVLGALASGMLLYGMSMLYGATGTLEITRLAEVIFDGSGREAVLIFALVFVVAGIGF
                     KLGAVPFHMWVPDVYHGAPTAVTVLIGSAPKLAAFAFIMRLLVQGLGAEPLLVEWQQM
                     LIVMAVLSLAIGNITAIAQTNLKRMLAYSTISHMGFLLLGILSGDLNGYGSGMFYAVV
                     YMAMSLGAFGMIMLLSRSGFEAENLEDFRGLNQRSPWYAFVMLLLMFSMAGVPPTVGF
                     YAKLSVLQAVINAGYVWLAVVAVLFALIGAFYYLRVVKLMYFDSPVDTTPIEPRTDVR
                     LVMSLNGLAMLILGIVPGPLMALCLYSIQVSL"
     gene            2609..3160
                     /locus_tag="VFY80_09305"
     CDS             2609..3160
                     /locus_tag="VFY80_09305"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6008148.1"
                     /translation="MAHTGDFTETTLATERVYGGGFFDVFRDEVRRPDGKPAVREYIK
                     HPGAVMMVPLLDERTVILIRQFRYPLQRHFIELPAGKMEVGEAPLATAQRELIEECGY
                     EAASWTHLTTLHPCIGYSDERIELYLARELTHVGSGPDDGEFLEVLPTPIDQALEWVR
                     AGRITEVKAIIGLLWLDRMPGRA"
     gene            complement(3188..>4974)
                     /locus_tag="VFY80_09310"
     CDS             complement(3188..>4974)
                     /locus_tag="VFY80_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6008149.1"
                     /translation="LIDRLASSLLMSGAEKSSRELVTIKRSLAEKRRIIQLLVESEHR
                     YRDLVETSHDLIWATDAQGRFTFLNNAAHDILGLAPKDLIGRCFFDFEAETSHVSNRR
                     FLAMLKRKGEVKNHVTHLRTAHGEDRWIGINARVVLDDHGTTFGIRGTARDITEQYLA
                     TQRIEHMALHDVLTDLPNRLSLQRGIETAMKDGSVGALLFLDIDHFKYVNDNFGHRAG
                     DQLIIGVGSVLREIARACAGELYRLGGDEFAIHLPGGLRQEATAAAEQALDGIAHYRF
                     QVSDQRVVANLSVSIGIALYPFHGDDLPTLLSNVDIAMYQAKDLGRNRYILSDQSSDN
                     LRSTHRRVHWAKRLRDALDEDRLILFSQPVVRLSDGKPVHHEILVRIREEDGQHIMPG
                     HFIEIAESLGLVQEIDLCVVEKLLAFMRRHDQIGKPLRYFVNLSRVSISDQRWIKRFI
                     RLLRDNRAHPSQLVFEITETAAMSEIDVTLAFIRQVKEMGCRFALDDFGAGFSSFYYL
                     KRFEVDYLKIDGSFIRDLAIDEGSRIFVRALNDVARGLSKQVIAEWVETPEVLRILTD
                     MGAQYGQGYLFQRPQPLERGVEPLRARA"
BASE COUNT          790 a         1522 c         1607 g         1055 t
ORIGIN      
        1 tgttctacgt gttcttcgag ggcatgctga tcccgatgtt catcatcatc ggcgtgtggg
       61 gcggccccaa ccgggtctat gctgcggtca agttcttcct ctatacgctc ctcggctcat
      121 tcctcatgct ggtggcgctg atctacctgt acaacgccgc gcagggcagc ttctcgctcc
      181 tcgattacta cgagttgccg ctttcgctcg ccgaacaggt tctgatcttc atcgcattct
      241 ttatggcctt tgccgtgaag gtgccgatgt ggccggtcca tacctggctt ccggacgcgc
      301 acgtcgaggc gccgaccggc gggtcggtcg tgctcgcggc gatcatgctg aagctgggcg
      361 gctacggctt tctccggctg agcctgccga tcctgcccga cgcgagccag tatctggccg
      421 gattcgtgat cgcgctttcg ctcatcgcgg tggtctacat cggtcttgtc gcgctcgtcc
      481 agtcggacct caaaaagctg atcgcgtatt cgtcgatctc gcacatgggt tttgtcacgc
      541 tcgggctctt catcttcaat ccacacggga tggaaggtgc ggtggtgcag atgatctcgc
      601 acggcttcgt gtcgggcgca ctgttcctct gcgtgggggt gctgtacgac cggcttcact
      661 cgcggcagat cgcggactat ggcggcgtgg tgaacacgat gccggtgttc gcggcgttct
      721 tcatgctgtt tgcgatggcg aactcgggac ttccgggtac gagcggcttc gtcggcgagt
      781 ttctcgtcat catgggcgcg gtgcaggtca acttctggta cgcgtttgcc gcggcctcga
      841 cgctcatctt cggcgccgcc tacacgctct ggatgtacaa gcgcgtgatc ttcggcgcgg
      901 tcgccaatgc ccgggttgca gcgctcaccg acatttctgc ccgcgaatgt ctcgtgctcg
      961 gcctgctcgc tgccgcggtc ctcgcgatgg gcatctaccc cgagcctttc gcgcgcgtgc
     1021 tgcacacctc ggtgaacgac ttgatcttcc acgcgactca gaccaagctg ccggccccgt
     1081 agcgagactc ccacatgcaa tacatcctgc ccctgtggcc cgcatatccc gagctcttcc
     1141 tgctcgtcat ggtctgcggc attctgctcg ccgatctcat cctgggcgac gacaggcgcg
     1201 ttgtcaccta tgcgctcaca cagcttgcac tcgccggtgc gattgcgatt tcgtggtgga
     1261 cggtgcgcat agagcccgcc tacaccttca gcggcatgtt cgtggatgac gtgatgtccg
     1321 acgtgctgaa gctcctcgtc tatctgggag tgatggggat actggtgtat tcccgcgctt
     1381 atctctccgc gcgcgggatg ttccgcgggg aattcttcgt gctgacgctg ttcgcgacgc
     1441 tcggcatgat gatcatgatc tcggccaacc acctgctcat cctgtacttg ggcctcgagc
     1501 tcatgacgct ttcactctat gcaatggtcg cgctccagcg cgactcggct actgcgaccg
     1561 aggcggcaat gaaatacttc gtgctcggcg cgctcgcttc cgggatgctg ctctacggca
     1621 tgtcgatgtt gtatggtgca accggaacgc tcgagatcac gcggctcgcc gaagtgattt
     1681 tcgacggcag cggccgggag gcggtgctca tcttcgcgct cgtcttcgtc gtggcgggca
     1741 tcggcttcaa gctgggggcg gtgccgttcc acatgtgggt gccggacgtc taccacggtg
     1801 ccccgacggc ggtcacggtg ttgatcggct ccgcaccgaa gctcgcggcg tttgccttca
     1861 tcatgcgttt gctcgtgcag gggctcggcg ccgagcccct cctggtcgag tggcagcaga
     1921 tgctgatcgt catggccgtc ctctcgctcg cgatcggcaa catcacggcg atcgcgcaga
     1981 cgaatctgaa gcgcatgctc gcctattcga cgatttccca catgggcttt ctgctgctcg
     2041 gcatcctgtc cggcgatctc aacggctacg ggtccggcat gttctatgcg gtggtctaca
     2101 tggcgatgag cctcggcgcc ttcggcatga tcatgctgct ctcgcgaagc ggcttcgagg
     2161 cggagaactt ggaagatttc cggggcctta accagagaag cccttggtac gcgttcgtga
     2221 tgctgcttct catgttttcc atggccggcg tgccgccgac ggtcgggttc tacgccaagc
     2281 tctcggtgct gcaggcggtc atcaatgcgg ggtacgtctg gcttgcggtg gtcgcggtcc
     2341 tctttgcgct gatcggcgcg ttttactacc tgcgcgtggt gaagctcatg tacttcgatt
     2401 cacctgtcga tacgacgcca attgagccac gcaccgacgt ccggctcgtc atgagcctca
     2461 acgggctcgc gatgctgatc ctcggcatcg tcccggggcc gctcatggcc ctctgcctct
     2521 actccatcca ggtctcgctg tagggcggcc gggtcaccca aaccggcgga cgacggggat
     2581 ttcgcgtccg taaacacgaa gggatctcat ggcgcataca ggcgatttca cagaaaccac
     2641 gcttgctacg gagcgcgtct atggcggcgg ttttttcgac gtcttcaggg acgaggtgcg
     2701 ccgcccggac gggaagcccg cggtacggga gtacatcaag cacccgggcg ccgtgatgat
     2761 ggtgccgctc ctcgacgagc gcacggtgat cctgatacgg cagttccgct atccgctgca
     2821 acggcacttc atcgagctgc cggccggcaa gatggaagtg ggcgaggcgc cgctcgcgac
     2881 ggcgcagcgt gagctcatcg aggagtgcgg ctacgaggcg gcgagctgga cacatctgac
     2941 gacgctgcat ccgtgcatcg gctattcgga cgagcgcatt gagctctatc ttgcgcgaga
     3001 actcacccac gtcggttcgg ggcccgatga cggcgagttt ctcgaagtgc tgccgacgcc
     3061 gatcgatcag gcgctcgaat gggtgcgcgc gggccgcatc acggaggtga aggcgatcat
     3121 cggactcctc tggctcgacc ggatgcccgg gagagcgtga gagcggggat ggcctcgggc
     3181 cgacggttca ggcgcgcgcg cgcagcggct ccacgccgcg ctcaagcggc tgcgggcgct
     3241 ggaagagata accctggccg tattgcgcgc ccatgtcggt caggatccgc aggacttccg
     3301 gcgtctccac ccattccgcg atcacctgct tgcttaagcc ccgtgccacg tcgttcagtg
     3361 cgcgtacgaa gatgcggctt ccttcgtcga tcgcaaggtc gcggatgaaa ctgccgtcga
     3421 tcttcaggta atcgacttcg aagcgcttga gataatagaa ggagctgaag cccgcgccga
     3481 agtcgtcgag cgcgaagcgg cagcccattt ccttcacctg ccggatgaac gcgagcgtca
     3541 cgtcgatctc cgacatcgca gcggtctcgg tgatctcgaa tacgagctgg ctcgggtggg
     3601 cgcggttatc gcggagcagc cggatgaagc gcttgatcca gcgctggtcc gagatgctca
     3661 cacgcgagag gttcacgaaa tagcgtagcg gcttgccgat ctgatcgtgc cggcgcatga
     3721 acgcgagaag cttttcgacg acgcagagat cgatttcctg caccagcccg agcgactcgg
     3781 cgatttcgat gaagtggccg ggcatgatgt gctgaccgtc ttcttcgcgg atgcgaacca
     3841 atatttcgtg gtgcacgggc ttgccgtcgg aaaggcgcac gaccggctgc gaaaaaagga
     3901 tcaggcggtc ctcatcgagc gcatcgcgca gccgcttcgc ccagtggacg cgccggtgag
     3961 tgctgcgcag gttgtccgag ctctggtcgg aaaggatgta gcggttgcgg ccgagatcct
     4021 ttgcctggta catcgcgata tcgacgttcg agagcagcgt cggaaggtcg tcgccgtgga
     4081 acggatagag cgcgatgccg atagagacgc tcaggttcgc gacgacccgc tgatcgctca
     4141 cctggaagcg gtagtgggcg atgccgtcga gcgcctgctc ggccgcggcg gtcgcctcct
     4201 gccgcagccc gcctggaagg tggatcgcga actcgtcgcc gcccagtcgg tagagctctc
     4261 ccgcgcaggc gcgggcgatc tcgcggagca cgctgcccac gccgatgatg agctggtcgc
     4321 ccgcgcggtg cccgaagttg tcgttcacgt acttgaagtg atcaatgtcc agaaagagga
     4381 gggcaccgac gctgccgtcc ttcatcgcgg tctcgatgcc gcgctgcagg ctgaggcggt
     4441 tggggagatc ggtgagcacg tcgtgcagcg ccatgtgctc gatgcgctgg gtggcgaggt
     4501 attgctcggt gatgtcgcgt gccgtgccgc gtatgccgaa cgtcgtcccg tggtcgtcca
     4561 gcacgacccg cgcgttgatc ccgatccagc ggtcctcacc gtgggcggta cggagatgcg
     4621 tcacgtggtt cttcacttcg cccttgcgct tcagcattgc aaggaatcgg cggttcgaga
     4681 cgtgcgaagt ctcggcctcg aagtcgaaaa agcagcggcc gatgagatcc ttgggcgcaa
     4741 ggccgagaat gtcgtgcgcc gcattgttga ggaaggtgaa gcggccctgc gcgtcggtgg
     4801 cccagatgag atcgtgcgag gtctcgacca agtcgcggta gcgatgctcg ctttcgacga
     4861 ggagctggat gatgcgtcgc ttttctgcca gggagcgctt gatggtgaca agttcgcgcg
     4921 agctcttctc ggcaccggac atgagcagcg atgacgcgag ccggtcgatc aaat
//
LOCUS       DASURG010000522         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_88936, whole genome shotgun sequence.
ACCESSION   DASURG010000522 DASURG010000000
VERSION     DASURG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88936"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFY80_09315"
     CDS             <1..1060
                     /locus_tag="VFY80_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6008150.1"
                     /translation="LAVPKEWGGPGADFVSQALVMAELAKGDSAISKAFSQNWKWSHL
                     IASACTEDQKQRFLKPFLADHRYVMGRGITEPNAGSDNRMPPEDDPKAGYRVRAVRQG
                     DEWILNGEKCFIANGSVGSLFFVDARTNPDVSIKEGGTLFMVPKGTPGFRIGKIFNKR
                     GWRFYQNAELIFENARVPHANVVGQVGTGSVKSGRGDTTGGDLFGDLELAANALGVCD
                     DAVEMAMHFSRTHKRAGKYLMEHQLIQLKLNEMHMLTEALRSYVMRVAWLHDRREHSA
                     NAGLVMNYSTDVIQRVTRLNMEIHGAEGCMINARIDKLVRDAMVWTHLAGDTVQRVKV
                     LKRLGKNGPGAAGMGTSD"
     gene            1306..2754
                     /locus_tag="VFY80_09320"
     CDS             1306..2754
                     /locus_tag="VFY80_09320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6008151.1"
                     /translation="MQPLLKVLLVEDAPADAELAIRTLQRAGIACESRIVANERELER
                     ELERFGPHIVLSDFSLPGFDGLGALRVVRERGADIPYIFVSGTIGEERAIDALRNGAT
                     DYVLKSNLARLPAAVTRAMREAEEARQRRRQDQKIARMDRVRGVQSGVSACIVRTRDR
                     RQLFQEICNIAAERGGFRMAWIGLTVADTMSVIPVAWSGHHASHIEAFVRRPAELLGE
                     QSAIGRAVRSRKEIVVNRIVAEPRLALRTQALQMGYGSMIALPLVCEDTVIGFWVIYA
                     AESDFFGVEETRLLSDVAADVSFALDYIAKQERLNELAYYDVLTGLSNRNLMHEHLRQ
                     ALAHAARNNRLVAVVFIDLDRFKWVNDTFGHSAGDRLLKEIAVRIASCTREGDIVSRH
                     GGDEFIMVLPDLPGCESATPVLQRVLESISEPVVIEGQDVCVSCSIGAAFFPHDGTDA
                     ETLLKHADAAMYEAKERGPGRLRYYGLEFDVA"
     gene            complement(2813..>3016)
                     /locus_tag="VFY80_09325"
     CDS             complement(2813..>3016)
                     /locus_tag="VFY80_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX6008152.1"
                     /translation="YRLRYSPAHRWFYFPEMRRDEALVFKVYDSEKDGRARFTAHTSF
                     HDPHTPPDAPPRQSIEARALAFF"
BASE COUNT          546 a          927 c         1022 g          521 t
ORIGIN      
        1 cctcgccgtg ccgaaggagt ggggcgggcc gggcgcggac tttgtgtccc aggcgctcgt
       61 aatggccgag cttgccaagg gcgacagcgc gatttcgaaa gccttcagcc agaactggaa
      121 atggagccac ctgatcgcct cggcctgcac cgaagaccag aagcagcggt ttctgaagcc
      181 atttcttgct gaccatcgct acgtcatggg acgcggcatc accgagccga acgcgggatc
      241 ggacaatcgc atgccgcccg aggacgatcc gaaggcgggc taccgcgtgc gggccgtgcg
      301 ccaaggagac gaatggatcc tgaacggcga gaagtgcttc atcgcgaacg gcagcgtcgg
      361 ctcgctcttc ttcgtggacg cgcgcaccaa tccggatgtc agcatcaagg aaggcggaac
      421 gctcttcatg gtgccgaaag gcacgccggg attccgcatc ggcaagatct tcaacaagcg
      481 cggctggcgc ttctaccaga atgcggaact catcttcgaa aacgcgcgtg ttccgcacgc
      541 gaacgtcgtg ggacaggtcg gcacgggatc ggtaaagtcc gggcgcggcg atacgacggg
      601 cggcgacctc ttcggcgatc tcgaactggc cgccaacgcc ctgggcgtgt gcgacgatgc
      661 ggtcgaaatg gcgatgcatt tttcccgcac ccacaagcgt gccggcaagt atctgatgga
      721 acaccagctt atccagctca agctgaacga gatgcacatg ctgaccgagg cgctgcgctc
      781 gtacgtgatg cgggtggcgt ggctccacga ccgcagggaa cattcggcga acgcaggtct
      841 cgtcatgaac tactccaccg atgtcattca gcgcgtgacc cgactcaaca tggagatcca
      901 tggcgcggaa ggctgcatga tcaacgcgcg catcgacaag ctcgtgcgcg acgcgatggt
      961 gtggacgcat ctcgcgggcg acacggtgca gcgcgtgaag gtgctgaagc gtctgggcaa
     1021 gaacggcccg ggcgccgccg gcatgggcac gagcgactga agcttcgccg cggcgatcac
     1081 gtcgcacgtt cagagtgaac gaccgttgtc cgcagtgtac ggttgcgtaa actgcatgcc
     1141 accgcgtgta gagatcgcgc cacacgctgt cgaacacgct ccacaccaat tccgtgatct
     1201 gatgccttgg cgcaatcacg gccctcgtat atcgttcctg ctgtctcttg catgtcccgc
     1261 cgtcggccgt cgcgacgacg gtgccaacgc cgcctggaat cgccgatgca accgctactc
     1321 aaagtgctcc tcgtggagga tgcacccgcc gacgcggaac ttgcgatccg tacgcttcag
     1381 cgtgccggca tcgcatgcga gagccgcatc gtcgcgaacg agcgcgagct tgaacgcgag
     1441 ctcgagcggt tcggcccgca tatcgtgctg tccgatttct cgcttcccgg atttgacggg
     1501 ctcggggcgc tgcgggtcgt gcgcgagcgc ggcgccgaca tcccgtacat cttcgtgtcg
     1561 gggacgatcg gcgaggagcg cgcgatcgac gcgctcagaa acggcgccac cgattacgtg
     1621 ctgaagagca atctcgcccg gttgcccgct gcggttaccc gcgccatgcg tgaagcggag
     1681 gaggcgcggc agcgccggcg gcaggaccag aaaatcgcgc gcatggatcg cgtgcgcggc
     1741 gtacagagcg gcgtgagcgc ctgcatcgtt cgcacccgcg accgccggca gcttttccag
     1801 gagatctgca acatcgcggc ggagcgcggc ggcttccgca tggcttggat cgggctgacg
     1861 gtcgccgata cgatgagcgt gatcccggtt gcgtggagcg gccatcatgc gagccacatc
     1921 gaggcattcg ttcggcgccc ggccgaactg ctcggcgagc agagtgcgat cggacgcgcc
     1981 gtgcggtcgc gcaaggagat cgtggtgaat cgcatcgtgg ccgagccccg cctcgcgtta
     2041 aggacgcagg cgctgcagat gggctacggc tcgatgatcg cgctgccgct cgtctgcgag
     2101 gacacggtga ttggattctg ggtgatctac gcggccgaat ccgatttttt tggcgtggaa
     2161 gagacgcggc ttctgagtga cgtcgcagcg gatgtatcgt tcgcgctgga ttacatcgca
     2221 aagcaggagc gcctgaacga gctcgcgtat tacgacgtgc tgacaggact ctccaaccgc
     2281 aatctcatgc acgagcacct gcgccaggcg ctcgcccacg ccgcgcggaa caaccgcctg
     2341 gtcgcggtcg tgttcatcga tctcgaccgc ttcaagtggg tgaacgacac cttcggccac
     2401 agcgcgggtg atcggcttct gaaggagatc gcggtgcgga ttgcctcctg cacgcgcgaa
     2461 ggcgacatcg tctcgcggca cggcggcgac gaattcatca tggtgctgcc cgatctcccc
     2521 ggctgcgaat cggcgacgcc ggtgctgcag cgggtgctgg agtcgatctc ggagccggtg
     2581 gtgatcgagg gtcaggacgt gtgcgtgagt tgcagcatcg gcgcagcgtt ctttccccat
     2641 gacggcacgg acgcggagac cctcctcaag cacgccgatg cggccatgta tgaggcgaag
     2701 gagcgcgggc ccgggcggct tcgctattac ggcctcgaat tcgacgtggc gtgatcagga
     2761 ctggctcgcg ccgggatgac gggcgcggcg gctttcgccg gatgcgggcg tgtcaaaaga
     2821 acgccaaggc gcgcgcctcg atgctctgtc gcggcggggc gtcgggcggc gtatgcggat
     2881 cgtgaaatga ggtgtgcgcg gtgaagcgtg cgcgtccgtc cttttccgaa tcgtagacct
     2941 tgaagacgag cgcttcgtcc cgtctcatct ccggaaagta gaaccagcgg tgcgcggggc
     3001 tgtagcgcag ccggta
//
LOCUS       DASURG010000523         7135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_294878, whole genome shotgun sequence.
ACCESSION   DASURG010000523 DASURG010000000
VERSION     DASURG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294878"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFY80_09330"
     CDS             complement(<1..330)
                     /locus_tag="VFY80_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008153.1"
                     /translation="MAARRPRIMMVGPWPPTKGGVTTFMRNVVRSPLGQKYDFVPFTT
                     SRPGKRNVTRDKYGYAAVFRGGVKRVLQGIAITLWHLVLYPWVVLVRRPAVIQIQASD
                     FQAFWEAA"
     gene            complement(354..2384)
                     /locus_tag="VFY80_09335"
     CDS             complement(354..2384)
                     /locus_tag="VFY80_09335"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX6008154.1"
                     /translation="MLEPDAAEAFWTPRESSSTKVRIAWYWNRLRCMSPPELGHRALR
                     TVRTRVEHWRSAGASPPRAHADAPFNAWIHVPSRPEVEHCIAAAERIAAGRFEIFALR
                     DVQLDSPPRWNVDPRTGVEAPMRFGKLINYRDPRIVGDIKYLWELNRHQHLVTLAQAH
                     AITGEARYFDILCAHLESWLAACPCPRGPNWSSALEAGLRLISWSAAWQLLGGSESPG
                     FADGQGRRLRERWLESVYQHARFIRGYFSLHSSANNHLIGEAAGLYIAAVTWPCWEEA
                     KRWRAEAKRILEREIVLQNGADGVNREQAVAYQRYELELLVLCALAGDANSETFSAAF
                     RARLLAMLEYIASIMDAGGNVPMIGDSDDAMVLRMDHRSSSCSYRALLATGAVLFRRG
                     DFKAKAGKLDERTRWLVTDADAIFRTLPTAGARLPVRRSFPEGGYYILGCDFETPQEI
                     RLLVDAGELGYQSIAAHGHADALSFWLSVGGVEFFVDPGTYAYHTQEAWRRYFRGTAA
                     HNTVRIDGADQSESGGNFMWVRKARAHCASWLPQPQCDVFTGWHDGYRRLASPVVHHR
                     SIRLDKRERRITIEDRLDAHGPHGVEVIFHCSEHCSVRPAADGYTVHAEGRSVRLNLP
                     RLNGAEQRVYYGSTAPIFGWVSRRLDERRPAPTIVWRCRISQSVQLRTEIFC"
     gene            complement(2371..4242)
                     /gene="asnB"
                     /locus_tag="VFY80_09340"
     CDS             complement(2371..4242)
                     /gene="asnB"
                     /locus_tag="VFY80_09340"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX6008155.1"
                     /translation="MCGIAGWIARPESALAEDTLAAMLDAIAHRGPDDEGTARFRCVS
                     GHHVFLGHRRLAIIDPEGAHQPMTDRDAGLALVFNGEIYNFRALRDELTRLGYSFVRD
                     SDTEVLLRAYQHWRDEVVAHLRGMFAFAVWDMRQERLFLARDRFGEKPLFVCQNGEDI
                     FFASEIKALLRVPGVEGAVDLEAVWEYLAYRYVPGPRTLFAGIRKLAPGSIAVWQRGR
                     YVERRYWRPPDRMAQAGASAPADAVPQFLDRLDEAVKSQMVSDVPFGAFLSGGLDSSA
                     IVALMSRHNSSVRTFSVGFGEDRYSELPYAAEVAAHFGTTHCEIVVAHRDIIEHLPRL
                     VAFRDAPVSEPSDIPIYLLAQEAARAVKMVLTGEGSDEILGGYPKHIVERYAAPYQRL
                     PRRLRRSLIGPLAHALPYGFRRAKTAVTNLNIEDWHERYVRWFGALDREERDRLSVLR
                     LNGTPRDDEPPFDAEPAASALRRILYFDQTSWLPDNLLERGDRMTMAASIESRVPFLD
                     QEVASYVSSLPDECRVRGWRSKWILREACRQLLPERILKRPKVGFRVPVNEWFRADLR
                     DYLLDHLQSAGSITRRYYDTATLDRVLAEHLEGRHNHEKLLWSLLNLEIWHRQYARA"
     gene            complement(4329..5678)
                     /locus_tag="VFY80_09345"
     CDS             complement(4329..5678)
                     /locus_tag="VFY80_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6008156.1"
                     /translation="MQEVLQRPQTANEYAPVTRLAQRAGTVAVVGLGYVGLPMAVEFG
                     QRRPTIGYDVSRKRIEGLRHGIDATREIAAPDLRKAEQLSFSDDPAQLGVADFVIIAV
                     PTPINAARQPDFGPLESASITVGRHMKPGAIVIYESTVYPGATEEVCVPLLERHSGMR
                     WRHDFHVGYSPERINPGDPDHSFSRITKVVSGDDPETLEKIADLYASVVSAGVYRASS
                     IRVAEAAKVIENTQRDLNIAFVNELAIIFDKLGLDTLEVLKAAGTKWNFLPYRPGLVG
                     GHCIGVDPYYLTHKAELAGYHPEVILAGRRINDGMGQHIARKTIQQMIHAGRNIKGAR
                     VNILGLTFKENCSDVRNSKVVDVIHGLREFEVETFVHDPVADAAEALHKYDIRLRSWD
                     ELPPADALILAVAHREFVERPVEAYLAKIVRSGCLIDVKSVFDPVAFRREGLQVWRL"
     gene            complement(5708..6811)
                     /locus_tag="VFY80_09350"
     CDS             complement(5708..6811)
                     /locus_tag="VFY80_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6008157.1"
                     /translation="MTTYEEVRMKLARGPRTWLVTGAAGFIGSHLTQALLDLDQNVIG
                     LDNFSTGHRRNLQQVRGAVGDARWRRFRLIDGDIRSLDTCRQACRAVDFVLHEAALGS
                     VPRSMEDPLTTHECNVNGFLNMLLAAREAGVERFVYASSSAVYGDHPRLTKIEAETGR
                     PLSPYAATKWMDELYAGIFARCFGFPSVGLRYFNVFGPRQDPNGPYAAVIPLWIAGMI
                     RNAPIYINGDGETARDFCYVDNVVQANLLAASVENASAVSEVYNIAAGDRTTLNELFE
                     LMRALLEPDHPHVREIRPIYRSFRPGDVRLSQADIAKARQQLGYQPIWRVREGLARAI
                     EWYATTMEAERDAALGQVAVQAALRGSGETVAP"
BASE COUNT         1312 a         2315 c         2280 g         1228 t
ORIGIN      
        1 cgcggcttcc cagaacgcct gaaagtcgct cgcctggatc tggatgacgg ccggccgccg
       61 caccagcacc acccagggat agagcacgag atgccacagc gtgatggcga tgccttgcaa
      121 cacgcgcttc acgcctccgc gaaagacggc ggcataaccg tacttgtcac gcgtgacgtt
      181 gcgcttaccc ggacgcgagg tggtgaacgg gacgaagtcg tacttctgtc caagcggcga
      241 gcgcacgacg ttgcgcatga acgtcgtgac cccgcccttg gtggggggcc atgggccgac
      301 catcatgatc cgtgggcgcc tcgcggccat gtcgcttgca tcgggcgttt gcatcagcag
      361 aagatctccg tcctgagctg cacgctttgc gaaatgcggc aacgccagac tatggtgggc
      421 gcgggccggc gctcatccag gcggcgcgac acccagccga atatcggggc cgtactgccg
      481 tagtacaccc gctgttcggc accgttcagc cggggaaggt tgagccgcac cgagcggcct
      541 tcggcgtgca ccgtatagcc gtccgcggcg ggacgaacgc tgcagtgctc gctgcaatga
      601 aaaatcactt cgaccccatg cggcccgtgc gcatccagtc tatcttcgat cgtgatgcga
      661 cgctcgcgct tgtcgagccg gatgctgcgg tggtgcacga ccgggctcgc gagccgacgg
      721 tagccgtcgt gccaccccgt gaagacatcg cattgcggct gcggcaacca tgacgcgcag
      781 tgtgcccgcg cctttctcac ccacatgaag ttgccgcccg actccgactg atccgcgccg
      841 tcgatgcgca cggtgttatg cgcggcggtg ccgcggaagt aacgtcgcca ggcctcctgc
      901 gtgtgatagg cgtaggtgcc gggatcgacg aagaactcta caccgccgac agagagccag
      961 aatgacagcg catcggcatg gccgtgtgcc gcgatcgact gatagcccag ttcgcccgca
     1021 tcgacgagga ggcgtatctc ctgcggcgtc tcgaagtcgc agccgaggat gtagtaccca
     1081 ccctcgggaa acgagcgtct gaccggcaat cgcgcccctg cggtggggag cgtgcggaaa
     1141 atcgcgtccg catccgtcac gagccagcgc gtgcgctcgt ccagcttacc ggccttcgcc
     1201 ttgaagtcac cgcgtcggaa taggacggcc ccggtcgcga gcagcgcacg atagctgcaa
     1261 gacgaagagc ggtgatccat cctgagcacc atggcgtcgt ccgagtcacc gatcatgggg
     1321 acgtttccgc ccgcgtccat gatcgaggcg atgtactcga gcatggcgag cagccgcgcc
     1381 cgaaacgcgg ccgagaatgt ttcgctgttc gcgtcgcctg ccagcgcgca caatacgagc
     1441 aactccagct cgtagcgctg ataggcaact gcctgttcgc gattgacgcc gtctgcaccg
     1501 ttctgcaaca cgatttcgcg ctcgaggatg cgctttgctt cggctctcca gcgcttcgcc
     1561 tcttcccagc agggccacgt cacggcggcg atgtaaagtc ctgcggcttc gccgatcaga
     1621 tgattgttcg ccgaggaatg cagcgagaag taaccgcgaa tgaagcgcgc gtgctggtac
     1681 actgattcga gccagcgctc gcgcaggcgc cggccctgcc cgtcagcaaa gccgggcgac
     1741 tcgctgccgc cgaggagctg ccaggcggcg gaccagctga tgagccgcag acccgcttcc
     1801 agcgcgctcg accagttggg acctctgggg cagggacacg ccgcgagcca gctctcgagg
     1861 tgcgcgcaca gaatgtcgaa atagcgtgct tcgccggtga tcgcgtgagc ttgcgcgagc
     1921 gtcacgaggt gctgatgccg gttgagctcc cacaggtact tgatgtcccc gacgatgcgc
     1981 ggatcgcgat agttgatgag cttcccgaac cgcatcggcg cctccacccc ggtcctagga
     2041 tcgacattcc accgcggcgg ggaatcgagc tggacgtccc tcagcgcgaa aatttcgaac
     2101 cgccccgccg caatgcgctc ggcggcagcg atgcagtgct cgacttccgg tcgggatgga
     2161 acatgaatcc acgcattgaa cggggcgtcc gcatgcgcgc gcggcgggga tgcgccggcg
     2221 gatcgccagt gctcgacccg cgtacgcact gtccgcagcg cacgatgccc gagttcgggt
     2281 ggcgacatgc agcgcagccg gttccagtac caggcgatcc gaaccttggt ggagctcgat
     2341 tcgcgcggcg tccagaaagc ttctgcggca tcaggctcga gcatattggc gatgccagat
     2401 ttcgagattg agcaacgacc agaggagctt ctcatggtta tgacgcccct cgaggtgttc
     2461 cgccagcacc cggtcgagcg tggctgtatc gtaatagcgt cgcgtgatcg aacctgcgct
     2521 ctgaaggtga tcgagcagat agtcgcggag atcggcacgg aaccattcgt tgaccggcac
     2581 gcgaaacccc accttcgggc gcttcagtat ccgctcgggc agcagttgcc gacacgcttc
     2641 gcgcaggatc cacttgctgc gccacccgcg cacccggcac tcatcgggca ggctcgacac
     2701 atacgaggcg acctcctggt ccagaaaggg cacgcgcgat tcgatcgatg ccgccatcgt
     2761 catgcgatcg ccgcgttcca gcaggttgtc cggtagccag ctcgtctgat cgaagtagag
     2821 aatgcgccgc agcgcgctcg ccgccggctc ggcatcgaac ggcggctcat cgtcgcgcgg
     2881 cgtgccgttg agccgcaata ccgagagtcg atcgcgctct tcgcgatcga gcgcgccgaa
     2941 ccagcgcacg tagcgctcgt gccagtcctc gatgttgaga ttcgtgaccg ccgtcttcgc
     3001 gcggcggaag ccgtaaggga gcgcatgcgc gagcggaccg atcagcgagc ggcgcagccg
     3061 gcgcggcagt cgctggtagg gtgccgcata gcgctcgacg atgtgcttgg ggtagccgcc
     3121 caggatttcg tcgcttccct cacccgtcag caccatcttg accgctcgtg cggcctcctg
     3181 cgcgagcaga tagatcggaa tgtcggaggg ctcggacacc ggggcgtcgc gaaacgccac
     3241 gagccgcggc agatgctcga tgatgtcccg atgggcgacc acgatctcgc agtgcgtcgt
     3301 gccgaaatgc gcggcgactt ccgccgcata gggcagctcg ctgtagcgat cttcaccgaa
     3361 tccgacggaa aaggtgcgca cgctcgaatt gtgccggctc atgagcgcga cgatcgccga
     3421 cgaatcgagg ccgcccgaca ggaaagcacc gaacggcacg tcgctaacca tctgcgattt
     3481 gacggcttca tcgagacgat cgagaaactg cggcacggcg tccgccggag cgctcgcacc
     3541 tgcctgagcc atgcgatccg gggggcgcca gtagcggcgc tcgacgtagc gcccgcgctg
     3601 ccacacggct atggatccgg gcgccagttt acggatcccg gcgaagagcg tgcgcggccc
     3661 aggaacgtaa cgataggcga gatactccca aaccgcctcc aggtcgacgg cgccttcaac
     3721 gccgggcacg cggagcagcg ccttgatctc cgaggcgaaa aagatgtcct cgccgttctg
     3781 acacacgaac agcggcttct ctccgaagcg gtcgcgcgcg aggaagagcc gctcctgacg
     3841 catgtcccag acggcgaacg caaacattcc gcgcaggtga gcgaccacct cgtcgcgcca
     3901 gtgctggtac gcgcgcagca gaacttcggt gtcggagtcc cggacgaagc tgtagcccaa
     3961 gcgtgtcagc tcgtcgcgca gtgcacgaaa attgtagatt tcaccgttga acaccagcgc
     4021 gagacccgca tcgcggtccg tcatcggctg atgcgcgccc tcgggatcga tgatcgcgag
     4081 ccggcgatgc ccgagaaaga cgtgatgccc ggaaacgcag cggaagcggg ccgtaccctc
     4141 gtcatccggg ccgcgatgcg cgatcgcatc gagcattgcg gccagcgtgt cctcggcaag
     4201 cgcgctttcg gggcgcgcga tccacccggc aattccacac atggcgcctc gtcaacgatt
     4261 gctcaagctg aaaagtggcg ctatgccaca ccccaagtcc gtgcgccgcg cgatccccgc
     4321 gcggcccgtc acagccgcca cacctggagg ccctcgcgcc ggaacgcgac cgggtcgaac
     4381 accgatttca catcgatgag gcagccggaa cgcacgatct ttgccagata ggcctcaacc
     4441 ggccgctcca cgaattcgcg atgcgccacc gcgagaatca gcgcatcggc tgggggcagc
     4501 tcgtcccagc tgcgcaggcg tatatcgtat ttgtgcagcg cctcggcggc atccgccacg
     4561 gggtcgtgga cgaaggtttc cacttcgaat tcacgcaaac cgtggatcac gtcgaccacc
     4621 ttggagttgc gcacgtcgct gcagttctcc ttgaacgtga ggccgagaat gttgacacgc
     4681 gcacccttga tgttgcggcc cgcgtggatc atctgctgaa tggtcttccg cgcgatgtgc
     4741 tggcccatgc cgtcgttgat gcggcgtccg gcgaggatca cttccgggtg atatccggcg
     4801 agctccgcct tgtgggtcag atagtatgga tcgacgccaa tacagtgccc accgacgagc
     4861 cccgggcgat acggcaggaa attccatttc gtgcccgccg ccttcagcac ttccagcgtg
     4921 tcgagaccga gcttgtcgaa gatgatcgca agttcgttca cgaacgcgat gttgaggtcg
     4981 cgctgggtgt tttcgatcac cttggcggct tccgcgacgc ggatggagga tgcgcggtac
     5041 acgccggccg acaccaccga agcgtaaaga tcggcgattt tttcgagcgt ttcaggatca
     5101 tcgccggaca ccaccttggt gatgcgggag aagctatggt cggggtcacc ggggttgatc
     5161 cgctccggtg aataaccgac gtggaagtcg tggcgccacc gcatgccgga gtgccgctcg
     5221 agaagtggaa cgcacacttc ctctgtcgcg ccggggtaga ccgtcgactc gtagatcacg
     5281 atggcgcccg gcttcatatg ccggcctacc gtgatgctgg ccgactcgag cgggccgaaa
     5341 tccggctggc gcgcggcatt gatcggcgtg ggcaccgcga tgatcacgaa gtcggccaca
     5401 ccgagctgcg cgggatcgtc gctgaacgag agctgctcgg ccttccgcaa gtcaggcgcc
     5461 gcgatctcgc gcgttgcatc gataccgtgg cgaagtcctt cgatacgctt gcgcgaaacg
     5521 tcgtaaccga tcgtggggcg gcgctgaccg aattcgacgg ccatgggcag ccccacatag
     5581 cccaggccaa cgaccgccac cgttccggcg cgctgcgcaa ggcgtgtaac cggtgcgtac
     5641 tcattagcgg tctgcggtct ttggagcact tcttgcatgg acctgtccct cgtgtcagca
     5701 caatgcacta cggtgcgact gtctctcccg agccccgcaa agcagcctga acggccacct
     5761 gccccaacgc agcgtcgcgc tcggcttcca tcgtcgtcgc ataccactcg atcgcccgcg
     5821 caagtccttc gcgcacgcgc cagatcggct gatatccgag ttgttggcgc gcttttgcga
     5881 tgtctgcctg ggaaagccgc acatcgccgg ggcgaaagga gcgatagatc ggacggattt
     5941 cgcgcacgtg cgggtggtca ggctcgagca gcgcgcgcat gagttcgaaa agctcattca
     6001 gcgtggtgcg gtcgcccgcc gcaatgttgt agacctcact cactgcagag gcgttttcca
     6061 cgctcgcggc cagcaggttc gcctgtacca cgttgtccac gtaacagaag tcgcgcgccg
     6121 tctcgccgtc gccgttgatg taaatgggcg cgttgcgaat catgcccgcg atccacaggg
     6181 gaatgacggc tgcataaggc ccgttcggat cctgccgggg accgaagacg ttgaagtagc
     6241 gcagtccgac cgagggaaag ccaaagcagc gggcgaaaat cccggcatag agctcgtcca
     6301 tccacttggt cgcggcatag ggtgaaagcg gccgcccggt ttccgcctcg atcttggtga
     6361 gccgcgggtg atcgccatac accgcgctcg agctcgcata gacgaaacgc tccacacccg
     6421 cttctcgcgc ggcgagcaac atattgagga agccgttgac gttgcactcg tgcgtggtga
     6481 gcgggtcttc catggagcgc ggcacactgc cgagcgccgc ttcgtgcagc acgaagtcca
     6541 cggcccggca cgcctgccga caggtatcca gcgagcggat gtcgccgtcg atcagccgga
     6601 atctacgcca tcgggcatcg cccaccgctc ccctgacctg ctgcaggttg cggcgatgcc
     6661 ccgtggaaaa attgtcgagt ccgatgacgt tctgatcgag atcgagcaac gcctgcgtga
     6721 gatgcgagcc gataaatccc gccgcgccgg tgacgagcca ggtgcgcgga ccgcgcgcaa
     6781 gcttcatccg gacttcctcg tatgtcgtca tgatctcctt tcatgttctg cgccgccgag
     6841 cgccttcggc acttggcaaa aacacgcagg cggcgaggga aaacagagcg attttcggca
     6901 tgctctgtgt ttccacgttg gcgcgaggga tagtccggat ggggcccgcg cagcgaggaa
     6961 ccggagtgcg cgcggtcccg atatatcctc ttgtccactg cggtcctagc gcttgccggc
     7021 gaccgcaagg ccactcatga aatttcctat gttgagaagc agcaggtacg cggcgccgag
     7081 cgtcgagccg cctaccacga gtctgatgaa gggggccggc tccgggaagt aggat
//
LOCUS       DASURG010000524         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_498030, whole genome shotgun sequence.
ACCESSION   DASURG010000524 DASURG010000000
VERSION     DASURG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498030"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1194
                     /locus_tag="VFY80_09355"
     CDS             58..1194
                     /locus_tag="VFY80_09355"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEX6008158.1"
                     /translation="MQIRIPCVLMRGGTSRGPFFLTDDLPADPAVRDEVLLAAMGSPH
                     DYQVDGIGGANPLTSKVAMISKSRHPGADVDYLFAQVLVNEKLVDTKPNCGNMLVAVA
                     PFAIEAGLVPAHHPETTVRIFNVNTKALVESIVQTPGGIVTYEGEAEIDGVPGTAAPV
                     KLNFRSAIGSVTGKLLPTGRPMDVIDGVEVSCVDVAMPMILMRAEQFGKTGHETAAEL
                     DADRALYARMEPIRRKAGEMMGMGDVSKLVVPKIGLLSKPRKGGTIASRYFVPYSCHK
                     AHAVTGTVCVASACAISGTVAAQIAPLTRAPQGIVKIEHPSGMIAIELDVELEKSPPV
                     MKRAALIRTARRIFDGHVHIPAAIWRGPQAASTRDERSAAASAA"
     gene            1223..2527
                     /gene="hpnE"
                     /locus_tag="VFY80_09360"
     CDS             1223..2527
                     /gene="hpnE"
                     /locus_tag="VFY80_09360"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEX6008159.1"
                     /translation="MNARLPHIAVLGGGYAGMAAAVTLAQAGVAVTVYEAASHLGGRA
                     RRVDYKGVTLDNGLHVLIGACSEVQKLVQLVNRDAARMLLRLPLDWHMVEGFRLRVRP
                     LPAPFHLAVALATARGLGWRARWAAARFIGALRAARFQLTDDVTVSALLAAHRQHESA
                     IRYLWQPLCASALNTPIDAASARVFVNVLRDGLAKAAAQSDLIVARENLTALFPEPAA
                     EYVQAHRGEVRTGHTVSDVVRRDGMFVVTAHGSAQAFTHVVCALSPQRLEAAIGRIVE
                     LRPALEKVAQFRYLPIHTISLQYANRVQMPAPMMGIGAGLAQWVFDREAICGQRGLLA
                     AVISAGGAHARLEQEELARRVDTELRRHFGPLPPLAWHRVIAEKRATFACTAGIERPS
                     ARTALEAFHLAGDYVESDYPATLEGAVRSGISAARAVLRTLG"
BASE COUNT          441 a          872 c          846 g          443 t
ORIGIN      
        1 gatgaggcgt ccatccctta taatcatgcc agctgttttc gactgggagc gcccatcatg
       61 caaatccgga taccatgcgt gctgatgcgc ggaggcactt cgcgcggccc gttttttctc
      121 accgacgatc tgccggccga cccggcggtg cgcgacgaag tgctgctcgc cgcgatgggt
      181 tcgcctcacg actatcaggt ggacggcatc ggcggcgcta atccgctcac gagcaaggtc
      241 gcgatgatca gcaaatcgcg ccatccgggg gcggacgtcg attacctctt tgcgcaggtc
      301 ctcgtgaacg aaaagctcgt ggacaccaag cccaattgcg gcaacatgct cgtcgcggtc
      361 gcgccattcg cgatcgaggc tgggctcgtg ccggcgcatc acccggaaac caccgtgcgc
      421 attttcaacg tgaacacgaa ggcgctggtg gaatcgatcg tgcaaacgcc gggcggcatc
      481 gtcacttacg agggtgaggc agagatcgac ggcgtgccgg gcaccgcggc gcctgtgaag
      541 ctcaatttca gaagcgcaat cggctcggtg accggcaagc tgctcccgac cggccgcccg
      601 atggacgtga tcgacggcgt ggaggtgagc tgcgttgatg tcgccatgcc gatgatcctc
      661 atgcgcgcgg agcagttcgg caagacggga cacgaaaccg cggcagaact cgatgccgac
      721 cgcgcgctct atgcgcgcat ggagccgata cggcgcaagg cgggagaaat gatgggcatg
      781 ggcgatgtat ccaagctcgt cgtgcccaag atcggtctcc tgtcgaagcc gcgcaaaggc
      841 gggaccatcg catcgcgtta cttcgtgcct tattcctgcc acaaagcgca tgccgtcacc
      901 ggcaccgtct gcgtcgcctc ggcgtgtgcc atatcgggca ccgtggcagc gcagatcgcg
      961 ccgctgacgc gcgccccgca ggggatcgtc aaaatcgaac acccctcggg gatgattgcg
     1021 atcgagctcg atgtcgagct ggaaaaatcg ccgccggtca tgaagcgcgc cgccctgatc
     1081 cgcaccgccc gccgcatatt cgacgggcac gtccatatcc cagcggcgat ctggcgcggc
     1141 ccgcaagcgg cttccacccg agacgagcgc tcagccgctg ccagcgcagc gtgacgcgcc
     1201 gagggcggcg cgacgcgaac gggtgaacgc gcgccttccc cacatcgccg tgcttggcgg
     1261 cggctatgcc ggcatggcgg cggccgtcac tttggctcaa gcgggcgtcg ccgttaccgt
     1321 atacgaagcc gcctcgcacc tcggcggacg cgcgcggcgc gtcgattaca agggcgtcac
     1381 actcgacaac ggccttcacg tcctcatcgg cgcatgcagc gaagtgcaga agctggtgca
     1441 gctcgtaaac cgcgatgccg cgcgcatgct gctccggcta ccgctcgact ggcatatggt
     1501 ggagggcttt cgcctgcgcg tgcggccgct gcccgcccca tttcatctcg ccgttgcgct
     1561 cgccaccgcg cggggcctcg ggtggcgggc ccgctgggcc gcagcccgat tcatcggtgc
     1621 gttgcgcgct gcacgtttcc aactgaccga cgacgtgacg gtctccgcct tgcttgccgc
     1681 gcaccggcag cacgaatcgg cgatccgtta tctgtggcag ccgctctgtg cgagcgcact
     1741 caatacaccg atcgatgcag catccgcccg cgtgttcgta aacgtcctgc gcgatggact
     1801 cgcgaaggcg gccgcgcaga gcgatctcat cgtcgcccga gagaatctga cggcgctctt
     1861 tcccgagccc gcggcggaat acgtgcaagc gcatcgcggc gaggtccgta cgggccatac
     1921 cgtgtcggac gttgtacgcc gcgacggcat gtttgtcgtc acggcacacg ggagtgcgca
     1981 agcctttacg cacgtcgtct gcgctctgtc cccgcagcgc ctggaagctg ctatcggccg
     2041 catcgtcgag ctgcgcccgg ccctcgaaaa agtggcgcag tttcgttacc tgccgatcca
     2101 taccatctct cttcaatacg ccaaccgcgt gcagatgccc gcgccgatga tgggcatcgg
     2161 cgcaggtctt gcgcagtggg tattcgatcg cgaggcgatc tgcggccagc gcggcctgct
     2221 cgccgcggta atcagcgcag gcggtgcgca tgcgaggctc gagcaggaag agcttgcgcg
     2281 gcgcgtggac acggagttgc ggcgacactt cggtccgctg cccccgctcg catggcatcg
     2341 cgtgattgcg gagaagcgtg cgacctttgc gtgcaccgcg ggcatcgaac ggccgtcagc
     2401 gcgcacggcg ctcgaagcgt tccatctcgc cggggattac gtcgaaagcg actatccggc
     2461 cacgcttgaa ggcgccgtga ggagcggcat cagcgccgcc cgcgcggttc tccggacact
     2521 cggttgagct gttcccggat cgattccggc agcggcgcag acatgccgag cgcgtggtcc
     2581 acgtacacga tcaccgcttc ac
//
LOCUS       DASURG010000525         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1110736, whole genome shotgun sequence.
ACCESSION   DASURG010000525 DASURG010000000
VERSION     DASURG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110736"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFY80_09365"
     CDS             complement(<1..192)
                     /locus_tag="VFY80_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008160.1"
                     /translation="MFALRLLAAVLAVLGVAPLWRLLRDPATGLAGAATAAQASAQSA
                     VLWSGLLLCLLPGIVAAALV"
     gene            complement(194..1006)
                     /locus_tag="VFY80_09370"
     CDS             complement(194..1006)
                     /locus_tag="VFY80_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6008161.1"
                     /translation="MSNIRLSVVMPVYNERNSVAASIQRVRNVPLDIEIICIDDGSQD
                     GTRDILQQLKGQGLIDQLILQPQNRGKGAAVRTGIQAATGDVIVIQDADLEYDPAEFP
                     DLLGPIADGRADAVFGSRFLGGPHRVLYFWHSVGNKFLTLISNMFSDLNLTDMETCYK
                     MVRADLMKSLPLTSNRFGIEPELTARLSQAQARIWEVPISYSGRTYAEGKKIGWRDGV
                     AAFWHIFKFNFFGPRTPVYGPPLTTSGTVLTPPAPAARDHTAVRTARSSSGN"
     gene            complement(1088..2452)
                     /locus_tag="VFY80_09375"
     CDS             complement(1088..2452)
                     /locus_tag="VFY80_09375"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6008162.1"
                     /translation="MAKVLIVDDERAILDTVEILLRGEGFDATVAQSGREAIERFDDI
                     SPDVVLTDIRMPGVTGLDLLEAVRQRDPEVPVILMTAQASLQSAVKAVNQGAFYYLQK
                     PFSNAELVALCKRAAQVRELSRENKTLKKELRRRDSSQEIKPVGKNRSFIEVLKLAET
                     VSPTDSTILITGESGTGKEVIARYIHQLSDREGGPFISINCGALPENLLESELFGHVK
                     GSFTGAVRDKEGLLVAAKGGTFFLDEIGEMSPALQVKLLRALQEREVVPVGATEHIEI
                     DVRIIAATNRDLEQEIRRGTFRSDLYYRLNVITLHLPPLRERKDDVQILAEYFLQRMA
                     AEQNGAGKQLRMDEEGLAALQRYDWPGNVRELENALERAAILTHDGLITVQHLPARIV
                     EPAPAPLISERPPANPTLELVERAYILWVLQAEGGNKARAAEVLGIDPSTLYRKLNRY
                     GVAD"
     gene            complement(2452..>3360)
                     /locus_tag="VFY80_09380"
     CDS             complement(2452..>3360)
                     /locus_tag="VFY80_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6008163.1"
                     /translation="GLGGVIRKTLSTSQPVRRYEIRMRNGAEERVLGLRTTLLERPGA
                     PWVTAVFQDITDSRQIEDLLRRTERLQAVAELGASLAHEIKNPLASIRSAVEQLDKSQ
                     LNDRDQSILRRLVVTESDRVSRLLSEFMEFSRVEVRRRSEVDVARIAADAIRLVEQHP
                     DAKGGASIEFAAPPHELIVDGDPDLLHRAVFNLVLNAVQHSGDNGVVRVEVSPVSKRE
                     IPPTVPAASVKLQVRDSGPGIPEEEVPRIFDPFFTTREGGTGLGLAMVHRAVEAHEGT
                     ILVDGHEGHGAQFTVYLPKQARRRNT"
BASE COUNT          615 a         1101 c         1022 g          622 t
ORIGIN      
        1 caccagtgcg gcagccacaa tgccgggcag caggcaaagc agcagaccgc tccacaacac
       61 cgcactctgc gccgacgcct gggccgcagt cgcggcacca gccagaccgg tagccgggtc
      121 gcgcagcaaa cgccaaagcg gcgcgactcc gagcacggcc agcacagcgg ccagtaagcg
      181 aagtgcgaac aactcagtta ccgcttgatg atcgggccgt gcgcaccgcg gtatgatcgc
      241 gggccgccgg tgcaggcggc gtgagcaccg taccggacgt ggtcagaggc gggccgtaaa
      301 cgggcgtgcg cggtccgaaa aagttgaact tgaagatgtg ccagaaggcc gcgacgccat
      361 ccctccagcc gatcttcttc ccttccgcgt aggtgcggcc ggaataggag atgggcactt
      421 cccagatgcg cgcctgcgcc tgcgacagcc gcgccgtgag ctcgggctcg atgccgaacc
      481 gattggatgt gagcggcagg ctcttcatca gatccgcacg caccatcttg taacacgtct
      541 ccatgtccgt gaggttgaga tcggagaaca tgttggaaat cagcgtcagg aacttgttgc
      601 ccacgctgtg ccagaaatag agcacgcgat gcggtccgcc caggaagcga ctgccgaaca
      661 cggcatcggc gcgcccgtcc gcaatgggac cgagcagatc gggaaactcg gcaggatcgt
      721 actcgagatc cgcgtcctga atgacgatca catcccccgt agccgcctgg atgccagtgc
      781 gcacggccgc gcctttgccg cggttctgcg gctgcaggat gagctgatca atcagcccct
      841 gccctttcaa ctgctgcagg atgtcacgtg tgccgtcctg cgaaccatcg tcgatacaaa
      901 tgatctcgat atcgagcggc acattgcgca cgcgctgaat ggacgcggcc acgctgtttc
      961 gctcgttgta aaccggcatc acgacactca gtcgaatgtt gctcaatcgc tcatctcagt
     1021 gaagggccaa ctgcccacag ggctgttggc gtgtattgcc atgcagcgct caatcgctct
     1081 gcggcgctca gtccgcgact ccgtagcggt tcagttttcg gtacagggtc gacggatcga
     1141 tgcccaggac ttcagcggcg cgtgccttgt tgccgccttc cgcctgcaat acccacagga
     1201 tatacgcacg ctcgaccagc tccagcgttg gattcgcggg cggccgctcg gagatgagcg
     1261 gcgccggtgc gggctcaacg atgcgtgccg gcagatgctg gacggtgatc aggccgtcat
     1321 gcgtgagaat ggcggcccgc tcgagcgcgt tctccaattc gcgcacatta ccgggccaat
     1381 cgtagcgctg caatgccgcc agtccttctt cgtccatgcg caattgcttg ccggcgccat
     1441 tctgctccgc ggccatgcgc tgcagaaagt actcggccag gatttgtacg tcatccttgc
     1501 gttcgcgcaa cggcggcagg tgcagagtga tcacgttcaa acgatagtag agatcgctgc
     1561 ggaacgtacc gcgccggatc tcctgctcga gatcgcggtt ggtggctgcg atgatgcgca
     1621 cgtcgatctc aatgtgttcg gtcgcgccca ccggcaccac ctcgcgctcc tgcaatgcgc
     1681 gcagcagctt gacctgcagc gcgggcgaca tctcgccgat ttcgtccagg aaaaaggtgc
     1741 cccccttggc ggcaacgagc agaccttcct tgtcgcgcac cgcaccggtg aagctgcctt
     1801 tcacgtggcc gaacaactcc gattcgagca ggttctcggg cagcgcgccg cagttgatcg
     1861 agatgaaggg accgccctcc cggtccgaca gctgatgaat gtagcgcgcg atgacttcct
     1921 taccggtgcc gctttcgccc gtgatcagaa tggtcgaatc cgtgggcgaa accgtttcgg
     1981 cgagcttcaa gacctcgatg aacgagcggt tcttgccgac cggcttgatc tcctgcgatg
     2041 agtcgcgacg ccgcagctcc ttcttcagcg tcttgttctc gcgcgagagt tcccgcacct
     2101 gcgccgcgcg cttgcacagc gcgaccagct cggcgttgct gaacggcttc tgcaggtagt
     2161 agaacgcgcc ctggttgacg gccttcaccg ccgattgcag tgaagcctgc gcggtcatca
     2221 ggatgaccgg cacctcggga tcgcgctgac gtaccgcttc cagcaggtcg agaccggtca
     2281 cacccggcat acgaatgtcg gtgagcacga cgtcgggtga gatgtcatcg aagcgctcga
     2341 tcgcctcgcg tccgctttgt gcgaccgtgg cgtcgaaccc ctcgcctcgc agcagaattt
     2401 ctaccgtgtc gagaatcgca cgctcatcat cgacgatgag taccttggcc attacgtgtt
     2461 cctccgtctt gcctgcttcg gcagatacac ggtgaattgc gcaccgtgtc cttcgtgacc
     2521 atccaccagg atggttcctt catgcgcttc caccgcgcga tgcaccatgg ccaacccgag
     2581 accggtgcca ccctcgcgcg tagtaaagaa aggatcgaaa atgcgcggca cttcctcctc
     2641 gggaatgccg ggcccgctgt cgcgcacctg cagcttgacg gaagccgccg gcaccgtggg
     2701 cggaatctcg cgcttgctca cgggcgacac ttccacgcgc accacaccgt tgtcaccgga
     2761 gtgctgcacg gcattgagca ccaggttgaa taccgcgcga tgcagcagat cgggatcgcc
     2821 atccacgatc agctcgtgcg gcggcgcagc gaactcgatg ctggcgccgc ccttcgcatc
     2881 cgggtgctgt tccaccagac gaatcgcatc cgccgcaatc ctggcgacgt cgacctcgct
     2941 gcgacgtcgc acctccacgc ggctgaattc catgaactcg ctgagcagcc gactgacgcg
     3001 atccgattcg gtgacgacca gacggcgcag gatgctttga tcgcgatcgt tgagctgtga
     3061 tttgtcgagt tgttcgaccg cgctcctgat ggaggcgagc ggattcttga tttcgtgggc
     3121 gagcgatgcg cccagctcgg cgacggcctg cagccgctcg gtgcgacgca gcagatcctc
     3181 gatctggcgg ctgtcggtaa tgtcctggaa aaccgcggtc acccaggggg caccgggccg
     3241 ctccagcaag gtcgtgcgca gacccagcac ccgctcctcg gccccgttgc gcatccggat
     3301 ttcatagcgc cgcacgggtt gcgaagtgct cagagtcttg cgaatcacgc cgcccaagcc
//
LOCUS       DASURG010000526         5886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_295454, whole genome shotgun sequence.
ACCESSION   DASURG010000526 DASURG010000000
VERSION     DASURG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295454"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFY80_09385"
     CDS             <1..823
                     /locus_tag="VFY80_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6008164.1"
                     /translation="TPVSLLDNTPLVELVKRHLDFSAIARAIERGDLLAFGVTCSGYT
                     SGQSVTFFQGRPELQSWKRSRRIGIAMPITVEHLVASSALPFIFPPVHINREFFGDGS
                     MRQIAPVSPALHLGANRLLVIGVGRQLVARELERTLTETHPSLAQIAGHALNSIFLDT
                     LESDLERLQRINRTIELIPADVLERTGYPLHHVEFRLMSPSVALEQIAARYAAELPRT
                     IRLLLGAVGATRQGGSNLLSYLLFERSYCRALMQLGYQDTMARKADLLEFLIGQQ"
     gene            944..1450
                     /locus_tag="VFY80_09390"
     CDS             944..1450
                     /locus_tag="VFY80_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX6008165.1"
                     /translation="MSRAFVKESDEDLAAGELPERPLSAHPNYVTPAGIERLQARVRE
                     LHERRERLHAEALEDPMGKQRLREVERDLRYFTTQLERAIVVDPAGQPQDEVHFGAVV
                     RISDEDGRVHQFSIVGDDEADVTAGKISWASPLAKAMIGSRVGDVVVWHRPAGDTEVA
                     ITDILYPK"
     gene            1447..2349
                     /locus_tag="VFY80_09395"
     CDS             1447..2349
                     /locus_tag="VFY80_09395"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEX6008166.1"
                     /translation="MTFLALIAALLLEQVRPLRPGNIVHALYARYAASLEAKFYGGKD
                     EHGVVAWALAVVVPAMLALALSLLLYRVSPLLAWMWHVTVLYFTMGFRQFSHYYGEIL
                     QALQDGHLERARESLSAWQGTPVPESASSEIARVAIELGLVSAHRHVFGPIACFVVLG
                     PAGAVLYRAAAMLAARWAGRGDPEGASAGRFAERFFYWLDWLPSRITAASFAIVGNFE
                     DSVYCWRTQAQAWAMQTQGIILSSGAGAIGVRLGDAAARNEAGTGDEADSDYMQSAIG
                     LIWRALVLWMFLLLLVSVAYLLGR"
     gene            complement(2363..3265)
                     /gene="rsgA"
                     /locus_tag="VFY80_09400"
     CDS             complement(2363..3265)
                     /gene="rsgA"
                     /locus_tag="VFY80_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX6008167.1"
                     /translation="MPDRSAAAGESVSLKGLVVASYGRRYAVEVSGAGAFDCVTRGKR
                     SDLACGDRVVITAGGPGQGVIEAVEPRASLLYRSVAHRQKLIAANVTQAVIVVAAAPP
                     PHEELLARCLAAAEHAGIPAAIVINKSDLPQHARAREALEIYRSLGYPVLSLSAKRDV
                     APLHPMLEGKMNVLVGQSGVGKSTIINALAPQARARTSELSRALGAGRHTTTHARLYH
                     LDAQTSIIDSPGMHAFGLHHIDPQALAAAFVEFRAEFGRCRYRDCLHAGEPGCAIASA
                     VDEGRIAKRRLDLYRALARKRDRY"
     gene            complement(3273..3608)
                     /locus_tag="VFY80_09405"
     CDS             complement(3273..3608)
                     /locus_tag="VFY80_09405"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6008168.1"
                     /translation="MHVADLASRKCRPCEGGVSPLRREEIDNLLKQLDGWMHAEGMIS
                     KTYTFSNYYQTMAFVNAVAWLAHREDHHPDLVVGYRECRVRYTTHAIGGLSENDFICA
                     AKVDALLSP"
     gene            complement(3646..4899)
                     /locus_tag="VFY80_09410"
     CDS             complement(3646..4899)
                     /locus_tag="VFY80_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6008169.1"
                     /translation="MTLFGLVFLAALALTTALRLWLAWRHIRHVRLHRGAVPAEFSGE
                     IGLDAHQRAADYTCAKTRLAMASIGVEALVVLALTFGGGVQALHHQAASWLAEGVGRG
                     LLLIALVTLIMTAIEQPFSYYRTFRIEQRFGFNRMTPSLFYMDLAKSALLAALFGLPL
                     AACVLWLMGKMGAHWWLYAWLVWVAFNVFMLAIYPTVIAPLFNRFSPMQDAELRERVE
                     RLLRRCGFRVKGLMVMDGSRRSSHGNAYFTGFGKSKRIVFFDTLLSRLTPAEVEAVLA
                     HELGHFKLRHVVKRMAWISAASLGFLWLLGYLARQEWFYRGLNVDIQSDAVALILFFL
                     VIPSFTFLLQPLGALYSRSHEFEADRYAADNSSAGDLVTALVKLYRDNASTLTPDPAH
                     SAFYDSHPPAVARIARLKSAAPARA"
     gene            4965..5510
                     /gene="orn"
                     /locus_tag="VFY80_09415"
     CDS             4965..5510
                     /gene="orn"
                     /locus_tag="VFY80_09415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX6008170.1"
                     /translation="MSQDPNSLIWIDMEMSGLNPEVDRVLEIAIVITDSALNATAEAP
                     VLVVHQPDAVLEAMDDWNKSMHGKSGLVDRVRASALSEAGAEEQMLAFLERYIAPGTS
                     PMCGNSVHQDRRFLAKHMPRLEGYFHYRNLDVSTLKELARRWKPEIMSGLAKHGKHEA
                     LADIYESIEELRYYREHFIKP"
     gene            complement(5520..5726)
                     /locus_tag="VFY80_09420"
     CDS             complement(5520..5726)
                     /locus_tag="VFY80_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX6008171.1"
                     /translation="MNDETLNMSIRKFLKTVGVNSQLAIEKAVRKAIEEGKLSGTESF
                     PATMTLTVGKLALEVKFDGDIKLG"
     gene            complement(5748..>5886)
                     /locus_tag="VFY80_09425"
     CDS             complement(5748..>5886)
                     /locus_tag="VFY80_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008172.1"
                     /translation="FDQWGVELGKQLASRVLPELKHDAAVAAHDPSTNGLINHYKAQR
                     R"
BASE COUNT         1064 a         1830 c         1936 g         1056 t
ORIGIN      
        1 cacgccggtc tcgctgctcg acaacacgcc gctcgtcgag ctcgtgaagc gccatctcga
       61 cttttccgcg attgcacggg ccatcgagcg cggcgatctt cttgcatttg gcgtgacgtg
      121 ctcgggatat acatcgggcc agtccgtcac gttcttccag ggacgccccg agctccagtc
      181 gtggaagcgc tcgcggcgta ttggcatcgc aatgcccatc acggttgagc atctcgtcgc
      241 ttccagcgcc ctgccgttca tctttccgcc ggtgcacatc aaccgcgaat tcttcggtga
      301 tggctccatg cggcagatcg cgccggtgag tccggcgctg catctcggag cgaaccgact
      361 gctcgtcatc ggcgtcgggc gtcagctcgt ggcccgcgag ctggagcgca cgcttaccga
      421 gacgcatccc tcgctcgcgc agattgcggg gcacgcactg aacagcattt tcctggacac
      481 gctcgagtcg gatctcgagc ggttacagcg cattaaccgc accattgagc tgatcccagc
      541 ggacgtgctc gagcgcaccg gctacccgct gcaccacgtg gaattccggc tgatgtcccc
      601 cagtgtggcc ctcgaacaga tcgctgcgcg gtacgccgct gagctgccgc gtaccatacg
      661 gctgctcctc ggcgccgtcg gtgcaacgcg gcagggggga tcgaatctcc tctcgtacct
      721 gctcttcgaa aggtcgtact gccgtgcgct gatgcagctc gggtatcagg atacgatggc
      781 gcgcaaggcc gatctgctcg aattcctgat cggccagcag tagggtgctg agcgggcttc
      841 gcttcgcgcg ggaacttcag tcagccggcg gtgtccgggc cgagagttgc catgccgtgc
      901 ggctctcggt ataaagaacg ttccactttg cgatcggtat acgatgagcc gtgcgttcgt
      961 caaggagagc gatgaagatc tggcagcagg cgagctgccg gaacggccgc tcagcgccca
     1021 tccgaactac gtgacccccg cgggtatcga gcggctccag gcgcgggtgc gcgagctgca
     1081 cgagcgcagg gagcggctgc acgccgaggc tctcgaagat ccgatgggca agcagcgcct
     1141 gcgtgaggtg gagcgggatc tgaggtattt caccacccag ctcgagcgtg caatcgtcgt
     1201 cgacccggcg ggacagccgc aggacgaggt gcatttcgga gccgtggtac ggatctcgga
     1261 tgaggacggg cgcgtgcatc agttcagcat cgtcggcgat gacgaggcgg acgtcaccgc
     1321 gggcaagatc agctgggcat cgccgcttgc aaaggcgatg atcggctcgc gagtcggtga
     1381 cgttgtggtg tggcaccgtc cagctggcga taccgaagtc gcaatcaccg acattctcta
     1441 tcccaagtga ctttcctggc gctgatcgcg gctctgctcc tcgagcaggt gcgcccgctg
     1501 cggccgggca acatcgtcca cgcgctctac gcgcgctatg ccgcgagcct cgaagcgaag
     1561 ttctacggcg gaaaggacga gcacggcgtc gtggcgtggg cgcttgccgt cgtggtgccc
     1621 gcaatgcttg cgctcgccct ttcgctgctc ctctatcgtg tgagcccgct cctcgcctgg
     1681 atgtggcacg tcaccgtact gtacttcacg atggggtttc gccagttcag tcactactac
     1741 ggcgaaattc tgcaggcgct ccaggacggg cacctcgagc gcgcgcggga gagcctgtcc
     1801 gcatggcaag ggacgcccgt gcctgaatcg gcgagcagcg aaattgcgcg cgtcgcgatc
     1861 gagttggggc tcgtcagtgc acaccggcat gttttcggtc cgatcgcgtg tttcgtcgtg
     1921 ctggggccgg cgggtgcagt gctgtaccgg gcggcggcca tgcttgcggc ccgctgggcc
     1981 gggcgcgggg accccgaagg cgcctccgcc ggccgctttg ccgagcgttt tttctactgg
     2041 ctcgactggc tgccgtcacg cattacggca gcgagctttg cgatcgtggg caatttcgag
     2101 gattccgtct attgctggcg cacgcaggcg caggcgtggg ccatgcagac ccaaggcatc
     2161 atcctctcaa gcggcgccgg tgcgatcggc gttcggctcg gtgatgctgc tgcacgcaac
     2221 gaagccggga ctggagacga ggccgactcc gattacatgc agagtgccat aggcttgatc
     2281 tggcgcgcgc tcgtgctgtg gatgtttctc ctgctgctcg tgagcgtggc gtacttgctc
     2341 ggtcggtgat gcgcggcagc agtcagtagc gatcgcgctt gcgtgcgagg gcacgataga
     2401 gatccaggcg tcgcttcgca atccgaccct cgtccacggc ggaggcgatg gcacaaccgg
     2461 gctcgcccgc atgcaggcaa tcgcggtagc ggcacctccc gaattcggcg cgaaattcga
     2521 cgaatgcagc tgcgagcgcc tgcggatcaa tatgatgcag gccgaacgcg tgcatgcccg
     2581 gcgaatcgat gatgctggtc tgcgcgtcca gatggtacaa ccgcgcgtgc gtcgtggtgt
     2641 ggcggccggc gcctagggcg cgcgagagtt cgctcgtgcg ggcgcgcgcc tggggcgcca
     2701 gcgcattgat gatcgtagac ttgccgacgc cggactgtcc caccagaacg ttcattttgc
     2761 cttcgagcat cgggtgcagc ggcgcgacgt cgcgttttgc gctgagcgac agcacggggt
     2821 aaccgaggct gcggtagatc tcgagcgcct ctcgcgcccg cgcgtgctgc ggcagatcgg
     2881 acttgttgat tacgattgcc gcaggtatcc cggcatgctc tgccgcggcg aggcaacgtg
     2941 cgaggagttc ttcgtgcggc gggggcgcgg cggccacaac gatcacggcc tgcgtcacgt
     3001 tggccgcaat cagcttctgg cggtgggcga cgctgcggta gaggagggac gcgcgcggct
     3061 ccaccgcctc gatcacgccc tgtccgggtc ctccggccgt gataacgacc cggtcgccgc
     3121 acgcaagatc gctccgcttg ccgcgcgtga cgcagtcgaa cgcgcctgcc cccgacacct
     3181 caaccgcgta acgtcggcca tagctcgcga ccaccaggcc cttgagcgaa acggattcgc
     3241 cggcggcagc gctgcggtcc ggcatcaaag catcacggcg agagcagcgc gtcgaccttg
     3301 gcggcgcata tgaagtcgtt ctccgagagg ccgccgatcg cgtgcgtggt gtaccggacg
     3361 cggcactcgc ggtatcccac cacgagatcg gggtgatggt cctcgcggtg ggcgagccac
     3421 gcgacggcgt tcacgaaggc catcgtctga tagtaattgg agaaagtgta agtcttgctg
     3481 atcatgccct cggcgtgcat ccagccgtcg agctgcttca ggaggttatc gatttcctcc
     3541 cgcctgagtg gcgacacacc gccttcgcag ggacggcact ttctgcttgc gagatcagcc
     3601 acgtgcatgg tgttctccgg gaaatggcaa cagtaaacga tgcgctcacg cgcgcgcggg
     3661 cgccgctgat ttcagacgcg cgatacgggc gactgccggc ggatgcgaat catagaacgc
     3721 ggaatgcgcg ggatccgggg tcaacgtgga ggcgttgtcg cggtagagct tcacgagtgc
     3781 ggtgacgaga tcgcccgccg acgaattgtc tgcggcgtag cgatcggctt cgaactcgtg
     3841 gctgcgggaa tagagcgcac caagcggctg cagcagaaat gtgaagctcg gtatgacgag
     3901 gaagaagagg atgagcgcaa cggcatcgga ctggatgtcc acgttgagcc cccggtagaa
     3961 ccactcctgc cgcgcaaggt agcccagaag ccaaagaaag ccgaggctcg cggcggatat
     4021 ccacgccatg cgctttacga cgtgccgcaa cttgaagtgc cccaattcat gggccagcac
     4081 cgcctcgacc tcggcgggcg tcaggcgcga gagcagcgta tcgaaaaaga cgatgcgctt
     4141 cgatttgccg aatcccgtga agtaggcgtt gccgtggctg ctgcgccgcg aaccatccat
     4201 caccatgagg cccttgacgc ggaagccgca gcgcctgagg agccgctcga cgcgctcgcg
     4261 caactcagcg tcctgcatcg gcgaaaagcg gttgaagagc ggcgcaatga ccgtggggta
     4321 gatcgccagc atgaagacgt tgaaagcgac ccaaaccagc cacgcataga gccaccagtg
     4381 cgcgcccatt tttcccatga gccaaagcac gcatgcggcg agcggcagac cgaacagcgc
     4441 ggcgagcaga gccgatttgg cgagatccat gtaaaagagg ctcggggtca tgcggttgaa
     4501 gccgaagcgc tgctcgatgc gaaacgtacg gtagtagctg aacggctgct ctatcgccgt
     4561 catgatcagc gtgacgagcg cgataagcag cagcccgcgg ccgacccctt ccgccaacca
     4621 cgacgccgcc tgatgatgta gcgcctgcac gccgccgccg aaagtgagtg cgagaacgac
     4681 gagcgcttcg acgccgatgc tcgccatggc gagccgtgtc ttggcgcatg tgtagtcggc
     4741 ggcacgctga tgcgcgtcga gcccgatctc gcccgagaat tcagccggga cggcgccgcg
     4801 atgcaggcgc acgtgccgta tatggcgcca ggcgagccac agccgcaatg cggtggtgag
     4861 cgcaagcgcg gcgaggaaga caaggccgaa aagggtcatc gcggtttcaa acgaggcccg
     4921 gaaaactgtg acacaatatc ggcctccaca attccaataa tattatgtca caggacccga
     4981 acagcctgat ctggatcgac atggagatga gcgggctcaa tcccgaagtc gaccgagtcc
     5041 tggagattgc cattgtaata accgattcgg cgctcaacgc gaccgccgag gcgccagtcc
     5101 tggtggttca ccagccggac gccgtgctcg aagcgatgga cgactggaac aagtcgatgc
     5161 acggcaagtc cggcctggtc gaccgcgtgc gcgcctctgc gctgtctgaa gcgggcgccg
     5221 aagagcagat gcttgctttc ctcgagcgct acatcgcgcc cggcacctca cccatgtgcg
     5281 gaaactcggt gcatcaggat cggcgcttcc tagcgaagca catgccgcgg ctcgaaggct
     5341 actttcacta ccgcaatctc gacgtgagca cgctgaagga actcgcgcgg cgctggaagc
     5401 ccgaaatcat gtcgggactc gcgaagcacg gcaagcacga ggcgctagcc gacatctacg
     5461 agtcgatcga agagctgcgc tactaccgtg agcatttcat caagccctga ccggctgcct
     5521 tagccgagct tgatgtcgcc gtcgaacttc acctcgagcg cgagtttgcc cacggtaagc
     5581 gtcatcgtgg cgggaaagga ttcggtgccg gagagcttgc cctcctcaat ggccttgcgc
     5641 accgcctttt cgatggcgag ctgggaattg acgcccacgg tcttgaggaa cttgcggatg
     5701 ctcatgttga gcgtttcgtc gttcatctgc atctccttga atgaaggcta gcggcgctga
     5761 gccttgtagt ggttgatgag gccgttcgtc gacggatcat gcgccgcaac cgctgcatcg
     5821 tgtttgagct cgggcaaaac ccggctggcg agctgctttc ccagctcgac cccccactgg
     5881 tcgaag
//
LOCUS       DASURG010000527         7879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1213140, whole genome shotgun sequence.
ACCESSION   DASURG010000527 DASURG010000000
VERSION     DASURG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213140"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..515
                     /gene="fabZ"
                     /locus_tag="VFY80_09430"
     CDS             54..515
                     /gene="fabZ"
                     /locus_tag="VFY80_09430"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX6008173.1"
                     /translation="MDIHQILELLPHRYPFLLVDRVLECEPGKRLVAIKNVTINEPFF
                     GGHFPHHPVMPGVLILEALAQAAAILSFKTINMRGDDRSVYYFVGIDKARFKKPVTAG
                     DTLRLEVTLQRYVRGIWKFWTEAHVAGKVVTEAELMCTVRSLKDGPAAGEP"
     gene            512..1285
                     /gene="lpxA"
                     /locus_tag="VFY80_09435"
     CDS             512..1285
                     /gene="lpxA"
                     /locus_tag="VFY80_09435"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX6008174.1"
                     /translation="MRVHPTAIVDPGARLAADVEIGPYAVIGGDVEIGPGCRIGAHTV
                     VTGHTRLGQNNRIYAFVSLGEAPQDKKYAGEPTRLEIGDHNTVREFCTLNRGTAQDVG
                     VTRIGDHNWIMAYSHVAHDCQVGNHTVFANNAQLAGHVHVGDYAILGGFTSVHQFCSI
                     GAHTITGLGTVVLQDIPPYVRAAGNSARPYGINAEGLRRRGYTPETIAAIKSAYRTLY
                     RSGLGLSEATHELERQNAQCPEIQPLLDFLAGSRRGIIR"
     gene            1294..2445
                     /gene="lpxB"
                     /locus_tag="VFY80_09440"
     CDS             1294..2445
                     /gene="lpxB"
                     /locus_tag="VFY80_09440"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX6008175.1"
                     /translation="MSPVVGIVAGEASGDLLGAHLIQSLKPHLPDARFVGIGGPRMRA
                     SGMDALFPMEKLAVRGYVEVLRHFREILRIRRRLAAYFLEHPPACFIGIDAPDFNLNL
                     ERRLKAAGIPTVQYVSPQIWAWRSERLHRIGRAVDKMLTVFPFEAPLYEAARIPVAYV
                     GHPLADMLGNFPDCSAAREQLRLPSAARIVALLPGSRVTELEQHADLFVATAMEIAAV
                     DPDVLFLAPLVSSETRALFEAALYRHDTPALNLTMLIGHAHEAMAAADVVLVSSGTAT
                     LEAALLRRPMVITYRMPRLSWWIMKRRFHERYVGLPNILCEQFVVPEILQDDATPENL
                     SQAVLNLMADAGVRARIDARFTRLLRELKQDTAQRVAGALLPMLQRSPA"
     gene            2442..3047
                     /gene="rnhB"
                     /locus_tag="VFY80_09445"
     CDS             2442..3047
                     /gene="rnhB"
                     /locus_tag="VFY80_09445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6008176.1"
                     /translation="MTVSTGTCGVDEAGRGPLAGPVYAAAVILPRSGRIEGLADSKTL
                     SAAKREALAVEIKSRARAWAVASASVSEIDGLNILRASLLAMQRAVQALGVAPRRVVV
                     DGLHCPQLPCPARAVVNGDATVAAISAASILAKVARDAFMVDLHQRYPQYGFDMHKGY
                     ATEFHLAALKAYGACEVHRRSFMPVSLVVQSGNLFPDDAWS"
     gene            3061..3516
                     /locus_tag="VFY80_09450"
     CDS             3061..3516
                     /locus_tag="VFY80_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX6008177.1"
                     /translation="MKIALMLHLLGTVVWIGGMFFAYFALRPSAALLLEPPQRLPLWC
                     ATLGRFFGWVWLAVVLVLASGFYMFAAIADAGRVPAYIHAMLYIGIIMTLIFGYVFFA
                     PYAALKRAVAAGNWAQAGAELNLIRIAVAINLALGMANIVVATAGALVR"
     gene            complement(3491..4465)
                     /gene="senB"
                     /locus_tag="VFY80_09455"
     CDS             complement(3491..4465)
                     /gene="senB"
                     /locus_tag="VFY80_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HEX6008178.1"
                     /translation="MRIALITPAPAGSRHGNRHTAARWAAMLRAMGHRLTVEQSWSGA
                     PAGLMIALHARRSHDSARRFKLAYPERSLIVVLTGTDLYRDIRCDDSARASLDLADRL
                     VVLQDHGLKELSRTLRHKARVIYQSAPHTPRRPPLESCFEVMVSGHLRHEKDPFRAAL
                     ALRLVPPQSRIRLVHLGAAMSDELAREARDWMAREPRYRWIGERSHAEALRRLARSRL
                     MVISSRMEGGANVVCEALAAGVPILASRISGNIGMLGADYAGYFPPENERALARLLWR
                     AESEPALYRLLQRQCAARRAVVDPRREARALKHLVVEAGRLTARARRR"
     gene            4515..5825
                     /gene="senA"
                     /locus_tag="VFY80_09460"
     CDS             4515..5825
                     /gene="senA"
                     /locus_tag="VFY80_09460"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HEX6008179.1"
                     /translation="MSRPHIDATALRALLAAARKRTLVLSSDLAAERLLGPKLRIVNP
                     PLWEIGHVGWFQERWCLRHAARSALKVSILDNADALYDSAAVAHDTRWTLPLPDFEAT
                     LAYLERVFHRVEERIEREGASEHLAYFVQLAAFHEEMHCEAFTYTRQTLGYPAPPDMR
                     ASAVEEGGALRGDAEIPGGTLMLGAGTEDGFVFDNEKWAHPVPIAPFRPARAAVTNAE
                     FLAFVEDGGYHRNALWSGEGWSWRVACGAEAPVYWRKDGADWLCREYDQWQRLAPRAA
                     VIHVNWYEADAYCRWAGRRLPSEAEWEWAAANTHAGTGKRRYPWGDAPPATPAVANLY
                     GAAGRAVDVGAYPGGESTWGCRQMFGNVWEWTSDWFGPYPGFVEDPYKEYSAPWFGDH
                     KVLRGGCHATRGSLLRNTWRNFYTPDRRDVFAGFRTCATDGERS"
     gene            5822..6616
                     /locus_tag="VFY80_09465"
     CDS             5822..6616
                     /locus_tag="VFY80_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX6008180.1"
                     /translation="MKVVRSRANPLFRTLLRLKDSPRERRRSGKTLLEGPQLVAEYME
                     HGGPLLALAASESALDAPEVKSLLARAKDTEAVVFPAALFREASSVTTPSGLVAVVPI
                     PQSVPASPDVACCVLLEAIQDPGNVGSILRSAAAAGVRHVLLSQGCADAWSPRVLRAA
                     MGAHYRLAIWERAELARYAHAFAGQVVVSDAAARRSIYDLDLTIPTAFAFGNEGAGLS
                     AGLRNEADIVAAVPMARGVDSLNVGAAAAVFLFERARQLEARRAPQ"
     gene            complement(6621..7445)
                     /locus_tag="VFY80_09470"
     CDS             complement(6621..7445)
                     /locus_tag="VFY80_09470"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX6008181.1"
                     /translation="MPQKRTAFFISDRTGITAEMLGHSLLTQFESVAFEEVTLPFVDS
                     VEKARQVVKQIDAYADKDGARPIVISTLVNSDIAEVVRSANALFLDCFDIFISPLEKE
                     LDVRASHAIGRSHSVHDFVNYHHRIESVNYTLSHDDGVTTTDLSQADIILVGVSRCGK
                     TPTCLYLAMQYGVRAANYPLVPDDFAAKQLPAHLRPLRSRLYGLTIRPERLQKIRNER
                     RPGSKYASLANCEYEVHEAETLMKLEGLPILDVTTKSVEELATTILHEAKLLRRIY"
     gene            complement(7538..>7879)
                     /locus_tag="VFY80_09475"
     CDS             complement(7538..>7879)
                     /locus_tag="VFY80_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6008182.1"
                     /translation="LPSSIFLIVVPFMPTLPLALLFLFLRSALSQMDVPARTSYVMAV
                     VSPAERPAAASLTAVPRSLAAAVSPALAGYLLSITVFGWPLIVCGTLKIVYDLLLLMM
                     FRRVTPPEEGR"
BASE COUNT         1348 a         2419 c         2700 g         1412 t
ORIGIN      
        1 cgttcgctgg agaagcggtt cgccgccttg ggaaggagca agccttgaac ggcatggaca
       61 tccaccagat actggaactc cttccgcacc gctatccgtt tctgctggtc gaccgcgtgc
      121 tcgagtgcga gcccgggaag cggctcgtcg cgatcaagaa cgtcacgatc aatgagccgt
      181 ttttcggcgg gcactttccg catcatccgg tgatgccggg cgtcctcata ctcgaggcac
      241 tggcgcaggc ggcggcgata ctctcgttca agacgatcaa catgcgcggc gacgaccgct
      301 ctgtctatta tttcgtcggc atcgacaagg cgcgcttcaa gaaaccggtg accgcgggcg
      361 acactttgag gctcgaagtg acgctgcagc gctatgtgag aggcatctgg aagttctgga
      421 ccgaggccca cgtggcaggc aaggtggtga cggaggcaga gttgatgtgc accgtgcgct
      481 cgctcaagga cgggccggcg gcgggcgagc cgtgagggta cacccgactg caatcgtcga
      541 tcccggcgcg cgtctcgcgg cagacgtgga gatcggcccg tatgccgtca tcggtgggga
      601 cgtggagatc gggccgggat gtcgtatcgg tgcccatact gtcgtcaccg gccacacccg
      661 cctcggccag aacaatcgca tctacgcctt cgtgtcgctc ggcgaagcgc ctcaggacaa
      721 aaagtacgcc ggcgagccca cccgtctcga aatcggcgat cacaacacgg tacgcgagtt
      781 ctgcacgttg aaccgcggca ccgcccagga cgtgggcgtg acgcggatcg gggaccacaa
      841 ctggatcatg gcgtactcgc acgtcgcgca cgattgccag gtgggcaacc acactgtctt
      901 tgccaataac gcccagctcg cggggcacgt gcacgtcggc gattacgcga tcctcggcgg
      961 attcacgtcg gtccaccagt tctgcagcat cggggcccat accatcacgg ggctgggcac
     1021 ggtggtgctg caggatatcc ccccgtacgt gagggccgcg ggcaattccg cccggcccta
     1081 cggcatcaat gccgaaggct tgaggcgccg gggctacacg ccggagacca tcgctgcgat
     1141 caagagtgcc taccggacgc tctatcgttc gggactcgga ttgagcgaag cgacgcacga
     1201 gctcgaacgc cagaatgcgc agtgccccga gatccagccg ctgctcgatt ttctcgcggg
     1261 ctcgcggcgg ggcatcatcc gctaggcgcc cgcatgtcgc cggtggtcgg gatcgtggca
     1321 ggcgaggcat cgggcgatct gctcggggcc catctcatcc agtcgctcaa accgcatctg
     1381 ccggacgcgc gcttcgtcgg catcggcggt cctcgcatgc gggccagtgg catggacgcg
     1441 ctgtttccca tggaaaagct cgcggtgcgt ggctatgtgg aggtgctgcg gcattttcgg
     1501 gagatcctgc gcatccgcag gcgcctcgct gcatacttcc tcgagcatcc gcccgcctgc
     1561 ttcatcggga tcgatgcgcc cgatttcaat ctcaatctgg agagaaggct caaggcggcc
     1621 gggataccga ccgtccagta cgtgagcccg cagatctggg cctggcgcag cgagcggctg
     1681 cacaggatcg ggcgtgccgt ggacaaaatg ctgacagtct ttccgttcga ggcgccactt
     1741 tacgaggcgg cgcgtattcc agttgcatac gtgggccacc cgctcgccga catgctgggc
     1801 aattttccgg actgcagcgc ggcccgcgaa cagcttcgac tgccgtccgc ggcccgcatc
     1861 gtcgcgctgc tgccgggaag ccgcgtcacc gagctcgagc aacatgcgga tctctttgtc
     1921 gcgacggcca tggagatcgc cgccgtcgat ccggacgtcc tctttctcgc gcctctcgtc
     1981 agcagcgaga cgcgcgcgct gttcgaggcc gcgctctatc gccacgatac accggcactc
     2041 aacctcacga tgttgatcgg gcatgcgcac gaagcgatgg ctgctgcgga cgtggtgctc
     2101 gtttcctccg ggacagcaac gcttgaagcc gcgctcctga ggcgcccgat ggtgatcact
     2161 taccgcatgc cgcgtctttc gtggtggatc atgaagcggc ggtttcatga gcggtatgtc
     2221 ggtctgccga acattctgtg cgagcagttt gtcgtgcccg aaattttgca ggacgatgcg
     2281 actccggaga atctatcgca ggcagtgctc aacctgatgg cggatgccgg tgtgcgcgcg
     2341 cgcatcgatg cacgcttcac ccggctcctg cgcgagctca aacaggatac ggcgcagcgg
     2401 gttgcgggag cgctgctgcc gatgctgcaa aggagccccg cgtgacagtc tcaaccggga
     2461 cctgtggcgt cgatgaagcg ggaagggggc cgctggcggg tccggtatat gcggccgcgg
     2521 tcatcctgcc gcgcagtggg cgcatcgaag gcctcgccga ttccaagacg ctttccgccg
     2581 caaagcggga ggcgctcgca gtggagatca agagccgggc gcgtgcctgg gcggtggcga
     2641 gtgcgagcgt ttccgagatc gacgggctca acatcctgcg cgcgagtctg ctcgccatgc
     2701 aacgcgcagt gcaggcgctc ggcgtggcgc cgcggcgcgt cgtggtggac gggctgcatt
     2761 gcccgcaatt gccctgcccg gcgcgcgcgg ttgtcaatgg cgacgcgacc gtggcggcca
     2821 tcagtgcggc ttccatcctg gcgaaggtgg cgcgcgatgc cttcatggtg gacttgcacc
     2881 agcgctatcc acagtacggg tttgacatgc acaagggcta tgcgaccgaa tttcacctcg
     2941 cggcgttgaa ggcctacggt gcttgcgagg tgcatcggcg cagcttcatg cctgtgagcc
     3001 tggtggtgca gagcggaaac ctttttccgg acgacgcctg gtcgtgaagg agaacatgcg
     3061 atgaaaatcg cgctcatgtt gcacctgctt ggaacggtcg tctggatcgg cggcatgttc
     3121 ttcgcatact tcgcgctgcg accgtccgcg gcgttactgc tggagccgcc ccaacggctg
     3181 ccgctatggt gcgccacgct cggacggttc ttcgggtggg tatggctagc ggtcgtgctc
     3241 gtgctcgcaa gcgggttcta catgttcgcc gcgatcgcag atgcaggccg ggtgccggcc
     3301 tacatccacg cgatgctcta catcggcatc atcatgacgc tgatattcgg atacgttttc
     3361 tttgcgccgt acgcggccct caaacgggcc gtcgcggccg ggaactgggc gcaggcgggt
     3421 gcggagctga atctcatccg catcgctgtg gcgatcaatc ttgcgctcgg catggcgaac
     3481 atcgtcgtag ctaccgccgg cgcgctcgtg cggtaagccg gccggcttca acgacgaggt
     3541 gtttgagggc gcgcgcttcg cgtcgcggat cgaccactgc gcggcgtgcc gcgcactggc
     3601 gctggagcag gcggtagagc gcgggctcgg attcggcgcg ccacaggagg cgtgccagcg
     3661 ctcgctcgtt ttcgggcgga aaataaccgg cgtaatcggc gcccagcatg ccgatgttgc
     3721 ccgagatgcg agaggcgagg atcggcacgc cggcggcgag cgcctcgcac acgacattcg
     3781 caccgccttc catgcgcgag gaaatcacca tgaggcggct acgcgcgagc cgccgcagcg
     3841 cctcggcgtg agacctttca ccgatccatc gatagcgcgg ctcgcgcgcc atccaatccc
     3901 gtgcttcgcg agccagctca tcgctcatgg cggcaccgag atgcacgagg cgtattctgc
     3961 tctggggcgg tacgagccgc agagcaagcg cggcacgaaa cggatccttt tcatgacgca
     4021 ggtgcccgct caccatcact tcgaagcacg actcgagcgg cgggcggcgc ggcgtgtgcg
     4081 gcgcagactg atagatgacc cgtgccttgt gacgcaacgt ccgagacaat tccttaagcc
     4141 cgtggtcctg cagcaccacg aggcggtcgg cgagatcgag cgaggcgcga gcggagtcat
     4201 cgcagcggat atcgcggtaa aggtcggtgc cggtcagcac cacgatgagc gaccgttcgg
     4261 gatatgcgag cttgaagcgg cgcgcggagt cgtgactgcg tcgggcatgg agcgcgatca
     4321 tgagcccagc gggcgccccg ctccaggact gctcgacggt gaggcggtgg cccatcgcgc
     4381 gcagcatcgc ggcccaacgc gcggcggtgt ggcgattgcc gtggcggctg cctgcaggcg
     4441 cgggtgtgat gagcgcaatt ctcacgctgt gtgatccacg ctaaactgcg gcgactatag
     4501 cagagcagca gagaatgagc cgaccgcaca tcgatgccac tgcgcttcgc gcgctgcttg
     4561 cagcggctcg caagcgcacg ctcgtgctct cgagcgacct cgcggccgag cgcctgctgg
     4621 gaccgaagct gcgcatcgtc aacccgcctt tgtgggaaat cgggcatgtc ggctggttcc
     4681 aggagcgctg gtgcctgcgt cacgcggcgc ggagtgcgct caaggtgtcc attctggaca
     4741 atgccgatgc gctgtacgat tcggctgcag tcgcacacga tacgcggtgg acgctcccgc
     4801 tgcccgattt cgaggcgacg ctcgcctacc tggaacgggt tttccatcgt gttgaggaga
     4861 gaatcgaacg tgagggcgcg agcgagcatc tcgcatactt tgtgcagctc gccgccttcc
     4921 acgaggagat gcattgcgaa gcgttcacgt atacccggca gacgcttggc tatcccgcac
     4981 cccctgacat gcgcgcctcg gctgttgaag agggtggtgc gttgcgcggc gatgccgaaa
     5041 tccccggcgg gacgctcatg ctcggagccg gtaccgagga cggtttcgta ttcgacaacg
     5101 agaagtgggc gcatccggtg ccgatcgctc cctttcgccc ggcacgcgcg gccgtgacga
     5161 acgcggagtt cctcgctttc gtcgaagacg gcggatacca caggaacgcg ctttggagcg
     5221 gtgaaggctg gagctggcgc gttgcatgcg gcgccgaggc gccggtttac tggcgaaaag
     5281 acggcgcaga ctggctgtgc cgcgagtacg accagtggca gcgtctcgcg ccgcgcgcag
     5341 ccgtcattca cgtcaattgg tacgaagcgg acgcatattg ccgctgggcg gggcggcgcc
     5401 tgccgagtga agccgaatgg gagtgggcgg cagcgaacac gcacgcggga acgggcaagc
     5461 ggcgctatcc gtggggtgat gcgccacccg caacgccggc cgttgccaat ctgtacggcg
     5521 cagcgggccg tgccgtggat gtcggtgcct atcccggcgg cgagagcaca tggggttgcc
     5581 gccagatgtt cggcaacgtg tgggagtgga cctcggactg gttcggtccg tacccgggct
     5641 tcgtggagga tccgtacaag gagtattccg cgccgtggtt cggagatcac aaggtgttgc
     5701 ggggcggctg ccatgccaca cggggcagcc tgctgcgcaa cacatggcgg aatttctaca
     5761 cgcccgaccg acgcgacgtg ttcgccgggt ttcgcacctg cgcgacagac ggcgaacggt
     5821 cgtgaaagtc gtccgctcgc gcgccaatcc gctgttcagg acgcttctca ggctcaaaga
     5881 ttctccgcgc gaacgcagac gttcgggcaa gacgctgctc gaaggaccgc agctcgtcgc
     5941 ggaatacatg gagcacggcg gtccgctgct cgcgcttgcg gcaagtgaaa gcgcgctcga
     6001 tgcgccggaa gtcaagtcgc tgcttgctcg agcaaaggac acggaggcgg tcgtcttccc
     6061 ggccgccctg ttccgtgaag catcctccgt caccacgcct tccgggctgg tggcggtcgt
     6121 gccgattccg cagtccgtcc cggcgtcccc tgacgtcgcg tgctgtgtgc ttctggaagc
     6181 gatccaggat ccgggaaacg ttggctccat tctgcggagt gccgcggcgg ccggcgtgcg
     6241 tcatgtgctg ctgtcgcaag gttgcgcgga tgcctggtcc ccgcgcgtgt tgcgcgccgc
     6301 gatgggcgcg cattaccggc ttgccatctg ggaacgtgcg gagctcgcac gctacgctca
     6361 tgctttcgcg ggacaggtcg tggtgagtga cgcggccgca cggcgatcga tatacgatct
     6421 cgatttgact attccgaccg catttgcctt tggcaacgag ggcgcgggac tttcggccgg
     6481 actgagaaac gaggccgata tcgtggcggc agtgccgatg gcgcgcggcg ttgattcgtt
     6541 gaacgtcgga gcggcggcgg cggtgttcct gttcgagcgc gcgcggcagc tcgaagcccg
     6601 ccgagcaccg caataggcgg tcagtatatc cggcgcagca gtttggcttc gtgcaggatg
     6661 gtggtggcga gctcctcgac ggacttcgtg gtcacatcga ggatcggcag gccctcgagc
     6721 ttcatgagcg tctcggcttc gtgtacctcg tattcgcaat tcgcgaggct ggcatacttg
     6781 ctgcccggcc ggcgctcgtt gcggatcttc tgcagccgct ccggacggat ggtgagaccg
     6841 tagagccgcg atcgcaaggg acgcaggtgc gcgggcagct gctttgcggc gaaatcgtcc
     6901 ggcaccagcg ggtaattcgc cgcgcgcacg ccgtattgca tggcgagata gaggcaggtc
     6961 ggtgtcttgc cgcagcgcga gacgccgacg aggatgatgt cggcctgtga gagatcggtc
     7021 gtcgtgacgc cgtcgtcgtg ggacagcgtg tagtttaccg actcgatccg atgatggtag
     7081 ttgacgaagt cgtgcacgct gtgactgcgc ccgatggcgt gcgaggcgcg cacgtcgagc
     7141 tccttttcga gcggcgagat gaaaatgtcg aagcagtcga gaaaaagggc gttggccgag
     7201 cgtacgactt cagcgatgtc ggagttgacg agcgtgctga tgacgatcgg gcgcgcgcca
     7261 tccttgtcgg cataggcatc gatctgcttg accacttggc gcgccttctc gacggaatcg
     7321 acgaatggca gcgtgacttc ctcgaaggcg acgctctcga actgggtaag caggctgtgc
     7381 ccgagcattt ccgcagtaat gccggtgcgg tccgagatga agaaggcggt acgtttctga
     7441 ggcatgacgg gcgtttcggg ctgcggctgc cgtaacatag cgtgttttcg aacgcgggac
     7501 aaacgcgcgg caggctcccg cgccgctcgc cggcgtgtca tcgaccctcc tcgggcggcg
     7561 tcacccgacg gaacatcatg agcagcagca agtcgtacac gatcttcagc gtgccgcaca
     7621 cgataagcgg ccaaccgaag acagttatcg agagcagata tccggcgagc gccgggctta
     7681 ccgcggcggc aagactgcgc ggcacagcgg tgaggctggc cgctgcgggg cgttctgcag
     7741 gagacaccac tgccatgaca tacgaagtgc gagcggggac gtccatctgc gatagcgcgc
     7801 tccggagaaa aagaaagagg agggcgagcg gcagcgtcgg catgaacggt acgacgatca
     7861 gaaaaatgct ggacggcag
//
LOCUS       DASURG010000528         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_295630, whole genome shotgun sequence.
ACCESSION   DASURG010000528 DASURG010000000
VERSION     DASURG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295630"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2126)
                     /locus_tag="VFY80_09480"
     CDS             complement(<1..>2126)
                     /locus_tag="VFY80_09480"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6008183.1"
                     /translation="SQAILGAATVRHDALYKYPAARADSTLVYTNLVPTGAFRGFGNP
                     SADWAVEQAWDLAAEKLGIDIVELLRINAVEPGDVSPHNHRITSCELRQCIDTAAALI
                     GWEAKRKARKPNRGLGMGCSVHVNGRRSFGDWDGSSAIVRINEDGRATVITGEGEIGQ
                     GNLTVLRQIAAETIGLPYEDVDVTRPDTDVQPHALGALASRLTYVAGNAVKNAAAAAA
                     KQLLEAAAEQFKRPAEELTVIGGQIGPRHGAETEFKPVGAVVRANIYKRGGQPIVGVG
                     NFDNPSEFPDHSRYGNESGAYNFVAQAVEVEVDPDTGRVKLLEIASCVDCGTVIHPAA
                     AEGQVHGAVTQGIGLAMIEYFDWYNGSPTDPQLKDYPIPSAAMMPKLHVAFAESYEPS
                     GPFGAKGLGEIGLDAIPAAIANAIADAVGVRIHELPITSEKIHRALHPERYADEKPAP
                     AAAPKGTAWNRLSAGKPSGARPANPEFVFARTVDEAVSLIANGDAAIVCGGTSHALRR
                     ERGGYPFAKRLVALNRIQELHALAIDETGTLHMGAAVCQESLYENRALRDRWPAIEDA
                     LEAVGHTRIRQMITVGGSIGPLIGGFDLPIALLALDASVTVAGPRGRRTLTLQDAFAN
                     RFEKDEIVVSVEVPPQRANTGSSFLKYMPRGVLEIPTVNTAARVTSDGNGGCAGARVV
                     VGSVSWKPIVLDLEALSGSRLGIEAA"
BASE COUNT          349 a          724 c          657 g          396 t
ORIGIN      
        1 tgcggcttca attccgagtc ggctgcccga tagcgcttcc agatccagca cgatgggctt
       61 ccagctcacc gagcccacca ccacccgcgc acccgcgcag ccgccatttc cgtcgcttgt
      121 cacgcgcgct gcggtattca ccgtcggaat ctcgagcaca ccgcgcggca tgtacttgag
      181 aaagctcgag cccgtgttcg cccgctgggg cggcacttcg acgctcacga caatctcgtc
      241 tttttcgaag cggttcgcga atgcgtcctg cagcgtgagg gtacggcggc cccgcggacc
      301 ggcgacggtc acgctcgcgt cgagtgcgag gagcgcgatc ggcagatcga agccgccgat
      361 gagcgggccg atgctgccgc ccaccgtgat catctggcga atgcgcgtgt ggccgaccgc
      421 ttcgagcgca tcctcgatcg cgggccagcg gtcacgaagc gcccggtttt cgtaaaggct
      481 ttcctggcac acggctgcgc ccatgtgcag cgtgcctgtc tcatcgatcg cgagcgcatg
      541 gagctcttga atgcgattga gagcgacgag gcgcttggca aacggatagc cgccccgctc
      601 gcggcgcagc gcatgcgaag tcccgccgca cacgatggcg gcgtcgccgt tagcaataag
      661 ggaaaccgct tcatccaccg tccgcgcgaa gacgaattcc ggatttgccg gacgcgcgcc
      721 cgaaggcttg cctgcggaga gccggttcca ggccgtgccc ttgggcgctg cggcgggtgc
      781 gggcttctca tccgcataac gctcaggatg cagcgcacgg tggatttttt ccgaggtgat
      841 gggcagttca tggatccgaa cgcccactgc atccgcaatc gcgttcgcga tcgccgccgg
      901 gatcgcatcg agcccgatct cgcccagtcc ctttgcgccg aacggaccgg acggctcgta
      961 tgactcggca aacgcgacgt gcagcttcgg catcatcgcg gcactcggga tcggatagtc
     1021 cttgagctgg ggatcggtcg gcgatccgtt gtaccagtcg aaatactcga tcatcgcgag
     1081 cccgatgccc tgcgtgacgg cgccgtgcac ctgcccttcc gcggcggcgg gatggatcac
     1141 cgttccgcaa tcgacgcacg aagcgatctc gagaagcttc acccgccctg tgtctggatc
     1201 gacctcgacc tccactgctt gcgcgacgaa gttgtaggcc cccgattcgt tgccgtagcg
     1261 gctgtgatcg ggaaattcgg aaggattgtc gaaattcccc acgccgacga tcggctgtcc
     1321 gcctcgcttg tagatattcg cgcgcaccac tgcaccgacc ggcttgaatt ccgtctccgc
     1381 gccatgacgc gggccgatct ggccgccgat gacggtgagc tcctccgccg gacgcttgaa
     1441 ctgctcggct gccgcttcga gcaattgttt cgccgccgcc gccgctgcat tcttgacggc
     1501 gttgcccgcg acgtatgtga gccgactcgc gagcgcaccc agcgcatgcg gctgcacgtc
     1561 ggtgtcggga cgggtcacat ccacgtcctc gtacggcaaa ccgatcgtct cggcggcgat
     1621 ctgccttaac accgtgagat tgccttgccc gatctcgcct tcgccggtga tgacggtagc
     1681 gcgcccatcc tcgttgatgc gcacgatcgc ggaactgccg tcccagtcgc caaaacttcg
     1741 ccgcccgttc acatggacgc tgcagcccat gcccagtcca cgattgggct tgcgcgcttt
     1801 gcgcttcgcc tcccagccga tcagcgccgc cgccgtgtcg atacactggc gcagctcgca
     1861 gctcgtgatc ctgtggttgt gcggcgatac atcgccgggc tcgacggcat tgattctcag
     1921 aagctcgacg atgtcgatgc ccagcttctc cgcggcgaga tcccacgcct gctccacggc
     1981 ccagtccgcg gaaggattgc cgaatccgcg gaaagcgccg gtcggaacga gattcgtgta
     2041 gacaagcgtc gagtcggcgc gcgcagcagg atatttgtag agcgcatcat ggcgcacggt
     2101 cgccgcaccg agtatcgcct gcgact
//
LOCUS       DASURG010000529         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1111373, whole genome shotgun sequence.
ACCESSION   DASURG010000529 DASURG010000000
VERSION     DASURG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111373"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFY80_09485"
     CDS             <1..477
                     /locus_tag="VFY80_09485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX6008184.1"
                     /translation="RFTRTLVRSERIANSAGASYDGIGRGPALFYLDGVPAGGTSVAQ
                     LEQALRREVRKVAEDGVSEEELKRIRAQVVAAHVYQRDSMFFQARQMGSMEAAGLSYK
                     TPDVILEKLKQVTAAQVQEVAKKYFGDDELTIAYLDPQPLADRKPAAPPPGARHGR"
     gene            467..1807
                     /locus_tag="VFY80_09490"
     CDS             467..1807
                     /locus_tag="VFY80_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6008185.1"
                     /translation="MGARVLRWIVCALFAVAAVPARATLPIQHWQTAAGTRVYFVENH
                     DLPMIDLSVEFPAGAAYDSPDNSGAASMTQRLLRLGAEGMNEEEIARGIADVGARLSG
                     SFDTDRAGLSLRTLSSREERDRALGIFARVLQKPVFPEAVLEREKLRLVAALKEADTK
                     PDTIAGRAFQRMLYRDHPYALRTTGEVATVQKLTRQDLADFHRRHYLSSRAVVAIVGD
                     LTRKEAERIAEQVTSGLPQDGGANAEIPAVPPLRDAATRLIPHPATQSHIAIGGLGIS
                     RHDPDYFPLVVGNHILGGGGFVSRLTEEVRTKRGLAYSVYSYFSPLLVRGPFQIGMQT
                     QREQAQQALDIVRAVLREFVAEGPTQTELTAAKKNIIGGFPLRIDSNRDIHGYLAIIG
                     FYRLPLTYLDDYIRNVEKLTVKDIRDAFARRVDPERMVTVVVAGGKDGGDTPAN"
     gene            1818..2393
                     /gene="rsmD"
                     /locus_tag="VFY80_09495"
     CDS             1818..2393
                     /gene="rsmD"
                     /locus_tag="VFY80_09495"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010113859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX6008186.1"
                     /translation="MASLRGGPSRGANRVRIIGGAWRSRVIRFSGSDEVRPTPDRVRE
                     TLFNWLGQDLTGACCLDLYAGSGALGFEAASRGARRVVMVERDAAAFRALEQSCTVLG
                     ARSVELVRADALEFLRSDSGSYDVVFLDPPFRSAEWPHLLAAVTTRLNSGALVYCESA
                     APFEIPAGWAVRRKSRAGQVCYQLLERTEHE"
     gene            2386..2880
                     /gene="coaD"
                     /locus_tag="VFY80_09500"
     CDS             2386..2880
                     /gene="coaD"
                     /locus_tag="VFY80_09500"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX6008187.1"
                     /translation="MNNKAVYPGTFDPMTLGHEDLVRRAARLFDTMILAIADSRAKQP
                     FFTLDERVEMATEVLKDVRNVKVMGFSGLLMEFVQQHKARVVIRGLRAVSDFEYEFQL
                     AGMNRGLYPDVETLFLTPGEQFMFVSATIVREISILGGDVSKFVHPHVAKRLKSKIRQ
                     LGGK"
     gene            2894..3157
                     /locus_tag="VFY80_09505"
     CDS             2894..3157
                     /locus_tag="VFY80_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX6008188.1"
                     /translation="MALLITDECINCDVCEPECPNEAISQGEEIYVIDPQRCTECVGH
                     YDEPQCRKVCPVDCIPLNPEWVEGRDALYAKYLRLTGQEEKQT"
     gene            complement(3154..>3408)
                     /locus_tag="VFY80_09510"
     CDS             complement(3154..>3408)
                     /locus_tag="VFY80_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX6008189.1"
                     /translation="PAGRIGLGRYRVLVCKVRETLELAIAARGSSLRDYVGSDGMAGS
                     YQNNFMVYDREGEPCYRCATPIRAIRQGQRSTFYCPRCQR"
BASE COUNT          624 a         1062 c         1132 g          590 t
ORIGIN      
        1 cgtttcacgc gcacgctggt gcgcagcgag cgcattgcca actctgcggg ggcgagctac
       61 gacggaatcg gccgcggccc ggcgctgttc tatctagacg gcgtgccggc gggcggcacc
      121 agcgtcgccc agctcgagca agccctgcgc cgtgaagtgc gcaaggtggc cgaagacggc
      181 gtgagcgagg aggagctgaa gcgcattcgc gcacaggtgg tcgccgcgca cgtctatcaa
      241 cgcgattcga tgttcttcca ggcgcggcag atgggttcca tggaggcggc ggggctgtcg
      301 tacaagacgc ccgatgtgat cctggaaaag ttgaagcaag taaccgcggc ccaggtacag
      361 gaagtggcca aaaagtactt cggcgatgac gagcttacga tcgcctatct cgatccgcag
      421 ccgctcgcag acaggaaacc ggcggccccg ccaccgggcg cgcgtcatgg gcgctagagt
      481 gctgcgctgg atcgtgtgcg cgttgttcgc tgtcgcggcc gtgccggcgc gcgccacgct
      541 gcccatccag cactggcaaa ccgctgccgg cacccgcgtc tacttcgtcg agaatcacga
      601 cttgccgatg atcgacctca gcgtcgagtt tcccgccggt gccgcctacg acagcccgga
      661 caactctggt gcggcgagca tgacgcagcg tctcctgcgc ctgggcgccg agggcatgaa
      721 cgaagaagaa atcgcgcgcg gcatcgccga cgtcggcgcg cggctttcgg gaagcttcga
      781 taccgatcgg gcggggctct cgctccgcac gctttcgagc cgcgaggagc gcgatcgcgc
      841 gctcgggatc ttcgcccggg tgctgcaaaa gccggtgttc ccggaggcgg tgctcgagcg
      901 cgaaaagctg cggctcgttg cggcgctgaa agaagccgac accaagcccg ataccatcgc
      961 gggccgcgcg tttcagcgca tgctctatcg cgaccatcct tatgccctgc gcaccacagg
     1021 agaggtggcg acggtgcaga aactgacgcg gcaggatctg gcggactttc atcgccgcca
     1081 ttacctttca tctcgcgcag tcgtggcgat cgtcggcgat ctcacgcgga aggaggccga
     1141 gcgcattgcc gagcaggtga cctccggcct gccgcaggac ggcggcgcga atgccgaaat
     1201 tcccgcggtg ccgccgctgc gtgatgccgc gacgcgtctc attccgcacc ccgccactca
     1261 aagccacatc gcgatcggtg gactcggcat cagccgccac gatccggatt attttccgct
     1321 cgtggtgggt aaccacatcc tgggcggtgg gggattcgta tcgcgcctga ccgaggaggt
     1381 gcggacgaag agaggactcg catactcggt gtacagctat ttcagtccgc tgctcgtgcg
     1441 cggccctttc cagatcggca tgcagaccca acgcgagcag gcgcagcagg cgctcgatat
     1501 cgtgcgtgca gtgctgcgcg aattcgtggc ggagggtccg actcaaaccg aactgaccgc
     1561 cgcaaagaag aacatcatcg gcggttttcc gctgcgcatc gacagcaacc gcgacatcca
     1621 cggctatctt gccatcatcg gtttctatcg actgccgctc acgtatctcg acgattacat
     1681 caggaacgtg gagaagctca cggtgaaaga catacgggac gccttcgcgc ggagagtcga
     1741 tcccgagcgc atggtgaccg tcgtcgtggc cggtgggaag gatggcggcg acacgcccgc
     1801 caactgaacg gcgaagcatg gcgtctttac gcggcggtcc gtcgcgcggc gccaaccgcg
     1861 tgcgcatcat agggggcgca tggcggagcc gagtgatccg cttcagcggc tcggacgagg
     1921 ttcgccccac gccggatcgc gtgcgcgaga cgctcttcaa ttggctgggt caggatctga
     1981 ccggcgcctg ctgtctcgat ctttatgccg gcagcggcgc gctcggtttc gaggccgcct
     2041 cgcgcggcgc gcgtcgtgtg gtcatggtgg agcgcgatgc ggcggcattt cgcgcgctgg
     2101 agcagtcgtg caccgtgctg ggcgctcgct cggtggaact tgtgagagcg gatgcgctag
     2161 aattcctgcg gagcgacagc ggctcttatg acgtcgtctt cctcgatccg ccgttccggt
     2221 cagccgagtg gccgcacctg ctcgccgccg tcacgacgcg attgaattcc ggcgccctcg
     2281 tctattgcga gagcgcggcg ccattcgaaa ttcccgccgg ctgggccgtg cgcaggaaaa
     2341 gccgtgcggg acaggtatgc tatcaactgc tcgaacggac ggaacatgaa taacaaggcg
     2401 gtctacccgg gaacgttcga tccgatgacg ctgggccacg aggatctcgt gcgccgcgcg
     2461 gcacgtttgt tcgatacgat gatcctcgcc atcgcggaca gccgcgcaaa gcagccattc
     2521 tttaccctgg acgagcgcgt ggaaatggcg accgaggtgc tgaaggacgt gcgcaacgtg
     2581 aaagtgatgg ggttttccgg gctgctgatg gaattcgtgc agcagcacaa ggcgcgcgtc
     2641 gtcatccgcg ggctacgcgc ggtgtccgat ttcgagtacg aattccagct cgcgggcatg
     2701 aaccgcgggc tctatccgga tgtcgaaacg cttttcctga cacccggaga gcaattcatg
     2761 ttcgtttccg ccacgatcgt ccgcgagatt tcgatactgg gcggggacgt aagcaagttt
     2821 gtgcatccgc acgtggcgaa gcgactgaag tccaagatcc gtcagctcgg cgggaagtag
     2881 agggcacgac cgaatggcgt tactgatcac tgacgaatgc atcaactgcg acgtctgcga
     2941 gcccgagtgc ccgaacgagg cgatttcgca aggcgaggag atctacgtga tcgacccgca
     3001 gcgatgcacc gaatgcgtcg ggcattacga cgagccgcag tgccgcaaag tatgtcccgt
     3061 cgactgcatt ccgctcaatc ccgaatgggt ggagggccgg gacgcgctct atgcgaagta
     3121 tctgcgcctc accgggcaag aggaaaaaca gacctagcgc tggcaacgcg ggcagtagaa
     3181 ggtcgagcgc tggccctggc gtatcgcgcg gatcggggtg gcgcagcgat agcacggctc
     3241 tccctcgcga tcgtagacca tgaagttgtt ctgatacgag cctgccatgc cgtcgcttcc
     3301 gacatagtcg cgcaggctgc tgccgcgcgc cgcgatcgca agctcgagcg tttcgcgcac
     3361 cttgcagacg agcacacgat agcgcccgag cccgatgcgg ccagccgg
//
LOCUS       DASURG010000530         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_703353, whole genome shotgun sequence.
ACCESSION   DASURG010000530 DASURG010000000
VERSION     DASURG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703353"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFY80_09515"
     CDS             <1..307
                     /locus_tag="VFY80_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class I"
                     /protein_id="HEX6008190.1"
                     /translation="GPRGKDFNMRWVASMVADVYRIISRGGIFMYPRDAKHKEGRLRL
                     MYEANPMALIVEQAGGAAIEGRQRILDVQPKALHQRIGVILGSRREVERVGQYYRDE"
     gene            complement(320..940)
                     /gene="msrA"
                     /locus_tag="VFY80_09520"
     CDS             complement(320..940)
                     /gene="msrA"
                     /locus_tag="VFY80_09520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6008191.1"
                     /translation="MNSLRIALVTLALTLGFVGTPVARAQGSPPSKPAAVAKATFAGG
                     CFWCMEEAFDPVPGVIATVSGYMGGKTRNPTYEQVSTGRTGHAEVVQIEYDPKRVTYE
                     KLLEVFWRNIDPTQRDAQFCDHGSQYRSAIFYHDAEQKRLAEASRSALVKSKPFKGEI
                     VTEITQAAEFYPAEDYHQDYYRKNPVRYKFYKTGCGRAARLKELWG"
     gene            complement(999..1763)
                     /locus_tag="VFY80_09525"
     CDS             complement(999..1763)
                     /locus_tag="VFY80_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6008192.1"
                     /translation="MAGSAALVDVLKRELKSRGITYARLARGLALSEASVKRMFSRRN
                     FTLVRIDAICHIAGIDFAELARAVSREEARISQLTYEHEKQIVSNKTLFLVAVCALHH
                     VTFEQIVKAFDITRAECIALLARLDRLKFLELQPGNRIRLLVSRTFSWRPDGPIQRFF
                     NEQAHNEYFRSRFDRPGEFMLVVNGMLTEASSIGVVDKLKRIAREFSAMHSEDVGAPL
                     GERSPMSLLVALRHWELQAFADMRRKDRARRVTRSV"
BASE COUNT          377 a          632 c          637 g          356 t
ORIGIN      
        1 cggtccgcgt ggcaaagact tcaacatgcg ctgggttgcc tccatggtgg cggacgtgta
       61 tcggatcatt tcacgcggtg gcatcttcat gtatccgcgc gacgcgaagc acaaggaggg
      121 gaggctcagg ctcatgtacg aggcgaatcc gatggcgctt atcgtggagc aggcgggcgg
      181 cgcggcgatc gaggggcgcc agcgcattct cgacgttcag cccaaagcgc tccaccagag
      241 gatcggcgtc atcctgggct ccagacgaga agtcgagcgc gtcgggcagt actatcgcga
      301 cgagtgagga catccacgct catccccaca gttccttcag tcgcgccgca cggccgcagc
      361 cggtcttgta gaacttgtag cgaacggggt ttttcctgta gtagtcctgg tgatagtcct
      421 cggcgggata gaactccgcc gcctgagtga tctcggtgac gatctcccct ttgaacggct
      481 tcgatttcac gagggcggaa cgggaggctt ccgcaagccg cttctgctcc gcgtcgtgat
      541 agaaaatggc gctccggtat tgcgagccat gatcgcagaa ctgggcatcg cgctgcgtcg
      601 gatcgatgtt gcgccagaac acttccagca gcttctcata ggttacgcgc ttcgggtcat
      661 attcgatctg gaccacttcc gcgtgtccgg tgcggccggt tgagacctgc tcataggtcg
      721 gattccgggt cttcccgccc atgtaaccgg acaccgtggc aatcacgccg ggcacggggt
      781 cgaaagcttc ctccatgcac cagaagcagc cgcctgcgaa ggtggccttc gccacagccg
      841 caggcttgga ggggggcgag ccctgagccc gggcgaccgg tgtcccgacg aaaccaagcg
      901 tgagcgcgag tgtgacgagc gcaatcctga gtgaattcat gccacctccg tgaaaaacgc
      961 gagcgtaaca ggcgttggtc gcgggcgtcg accgggcatt acacggagcg ggtgacgcga
     1021 cgcgcccggt ctttgcgccg catgtcggca aacgcctgca actcccagtg gcggagcgcg
     1081 acgagcaggc tcatcggcga acgctcgccg agcggcgcgc cgacgtcctc gctatgcatc
     1141 gccgagaact ccctcgcaat gcgcttcagt ttgtcgacga cgccgatact cgatgcctcg
     1201 gtcagcatgc cgttaacgac cagcatgaac tcgccggggc ggtcgaagcg cgatcggaag
     1261 tactcattgt gcgcctgctc gttgaagaaa cgctggattg ggccgtcggg tcgccatgag
     1321 aacgtgcgcg acacgagcag tctgatgcgg tttcccggct gcagctcgag gaacttcagg
     1381 cgatcgaggc gcgcgagcaa cgcgatgcat tccgcccgcg tgatatcgaa cgccttgacg
     1441 atctgctcga aggtgacgtg gtggagggcg cagaccgcga cgagaaagag cgtcttgttg
     1501 gaaacgatct gcttttcatg ttcgtatgtc agctgcgaga tgcgcgcctc ctcgcggctc
     1561 acggcgcgcg caagctcagc gaagtcgatg ccggcaatgt gacagatcgc gtcgatgcgc
     1621 acgagcgtga aattccggcg cgaaaacatt ctcttcacgc tcgcttcgct cagcgcgaga
     1681 ccccgggcga gccgcgcata ggtgatgcca cgggacttaa gctcgcgctt cagcacatcg
     1741 accagcgccg cgcttcctgc catgtctttc ctccccgcgc ctcccccggc gcggtaaaaa
     1801 agtagcacct aaggctaccc gcgggcaaac cggcggcaac ctcccctcgc acgcttgccg
     1861 cgccgggcag cggtctccag aatgccgcca cgcgcccgga ggaggcgcat cccatccgca
     1921 ggaggagccc atgacggaag atccgagcga cgacctgggc ggccgtcggg attcaccatc
     1981 catccacaga cttctcacgc gg
//
LOCUS       DASURG010000531         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_296037, whole genome shotgun sequence.
ACCESSION   DASURG010000531 DASURG010000000
VERSION     DASURG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296037"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VFY80_09530"
     CDS             complement(<1..669)
                     /locus_tag="VFY80_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6008193.1"
                     /translation="MSAVELRASIGLAAIFGLRMLGMFAILPVFAIYAERLPGGNDLT
                     LVGIAIGAYGFTQAILQIPFGWWSDRYGRRPVIYTGLAIFAVGSFIAALGHHIYVVIL
                     GRILQGSGAISAAVMALAADLTREEHRTKAMAMIGSTVGATFALSLIAGPALDRAIGV
                     PGIFVMTGVLALAAIGVAYAVVPATQGEPATGREQDGVRFGVVLADPQLLRLNYGVFA
                     LHAAL"
     gene            831..>2437
                     /gene="uvrA"
                     /locus_tag="VFY80_09535"
     CDS             831..>2437
                     /gene="uvrA"
                     /locus_tag="VFY80_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6008194.1"
                     /translation="MRIRGARTHNLKNISLDLPRNRLVVITGVSGSGTSSLAFDTLYA
                     EGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKAASHNPRSTVGTITEIH
                     DYLRLLFARVGDPHCPDHRMTLTAQTVSQMVDHVLELPEDARLMILAPLIVGRKGEQA
                     ELFDELRAQGFVRVRIDGTVHEIDALPQLKKNVKHTVEVVVDRLRVRADAKQRLAESF
                     ETALRHADGRAVAVEMESGAEHLFSAKFACPVCSYSLPELEPRLFSFNNPMGACPRCD
                     GLGTVSFFDPKRVVAFPELSLASGAIKGWDRRNQFYFQMLQCLATHYGFDLEAAFNEL
                     PQNVQEAVLHGSGHDKIRFVYVGERGNKFVREHAFEGVIPNLERRYRETDSVVVREEL
                     AKYLNSQACPECNGTRLRREACNVTVAGRTIYEVSQLPLAQAVPFFETLELSGAKQAI
                     ADKIVREILNRLQFLNNVGLDYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVMYVLD
                     EPSIGLHQRDNVRLLDTLKRLRDIGNSV"
BASE COUNT          459 a          784 c          783 g          411 t
ORIGIN      
        1 caacgcggcg tgcagggcaa atacaccata gttgagtctc aggagctgcg gatcggccag
       61 cacaacgccg aatcggacac catcctgctc gcgcccggtc gccggctcgc cctgcgttgc
      121 cggaaccaca gcgtaggcga cgccgatcgc tgcgagcgcc agcacgccgg tcatcacgaa
      181 aattcccggc acgccgatcg cgcgatcgag cgcgggacca gcgatgagcg atagcgcgaa
      241 ggttgcgccc accgtcgagc cgatcatggc catcgccttg gtgcgatgct cctcgcgcgt
      301 gaggtcggcc gcgagcgcca tcaccgcggc ggaaatggcg ccggaaccct gcaggatccg
      361 accgagaatg acgacgtaga tgtgatgccc cagcgcggcg atgaagctcc ccacggcaaa
      421 aatcgcaaga cccgtataga tcacggggcg gcggccatag cgatccgacc accagccgaa
      481 cgggatctgc aggatcgcct gcgtgaaacc gtacgccccg atcgcgatgc cgacgagtgt
      541 cagatcgttg ccgccgggga gacgctcggc atagatggcg aataccggca ggatcgcgaa
      601 catgccgagc atgcgcagac cgaagattgc ggcaaggccg atgctcgcgc gcagctcgac
      661 tgcgctcatg cgctcgctgg tggacatggg cgggaggctg agcgggttgg gatttgcgcg
      721 caaagtccgg tatattaaca ggtttgacta cgccgacccg cttgaaaccg aatcctgatt
      781 acgccgtcgt cgcgctccac acgccggggg cggtgcgcgg cggagagttc atccgcatcc
      841 gcggcgcgcg cacccacaat ctcaagaaca tcagtctcga tctgccgcgg aaccgcctcg
      901 tcgtgatcac gggcgtttcc ggctcgggca cgtcctccct tgcgttcgac acgctctacg
      961 cagaaggcca gcgccgctat gtcgaatcgc tgtcggccta cgcccggcag ttcctgcagt
     1021 tgatggaaaa gcccgacgtc gatctcatcg aggggctttc gcccgcgatt tcgatcgagc
     1081 agaaggccgc aagccacaac ccacgctcga cggtgggcac gatcactgag attcacgatt
     1141 acctgcggct gctcttcgcg cgcgtgggcg acccgcattg ccccgatcac cggatgacgc
     1201 tcaccgcgca aaccgtctca cagatggtcg atcacgtgct cgagcttccc gaggatgcgc
     1261 ggctcatgat cctcgctccg ctcatcgtcg gacgcaaagg cgagcaggcg gagctcttcg
     1321 atgagttgcg cgcgcaggga ttcgtgcgcg tgcgcatcga tggtacggtg catgagatcg
     1381 atgcgcttcc gcagctcaag aagaacgtga agcacacggt ggaagtggtg gtggatcgcc
     1441 tgcgcgtgcg ggcggatgcg aagcagcggc tcgcggaatc gttcgagacg gcgctccgac
     1501 atgccgacgg ccgcgccgtc gcggtcgaaa tggagagcgg cgccgagcat ctcttctctg
     1561 cgaaattcgc ctgcccggtg tgcagctatt ccctgccgga gctcgagccg cggctcttct
     1621 cgttcaacaa tccgatgggc gcgtgcccgc gctgcgacgg cctcggcacg gtaagctttt
     1681 tcgatccgaa gcgggtcgtc gcgtttcccg agctctcgct cgcgtcgggc gcgatcaagg
     1741 gctgggatcg acgcaaccag ttctacttcc agatgctgca gtgtctcgcg acgcactacg
     1801 gtttcgatct agaggccgca ttcaacgagc tgccgcagaa tgtacaggaa gcggtattgc
     1861 acggctcggg gcacgacaag atccgtttcg tgtacgtcgg tgagcgcggc aacaagttcg
     1921 tgcgcgagca tgcgttcgaa ggcgtgatac cgaatttgga gcgccgctat cgcgagaccg
     1981 attcggtcgt ggtgcgcgag gaactcgcga agtatctcaa cagccaggca tgccccgagt
     2041 gcaacggaac gcggcttcgg cgcgaagcct gcaacgtgac ggtcgccggc cgcacgattt
     2101 acgaggtgag ccagctgccg ctcgcgcagg ccgtgccgtt cttcgagacg ctggaattga
     2161 gcggcgcgaa gcaggcgatc gccgacaaga tcgtgcgcga aatcctgaat cggctgcagt
     2221 tcctcaacaa cgtcgggctc gattacctct cgctcgaccg ctccgccgac acgctttcgg
     2281 gaggcgaggc gcagcgcatc cggctcgcaa gccagatcgg ctccgggctc acgggcgtaa
     2341 tgtacgtcct cgacgagcct tcgatcgggt tgcaccagcg cgacaacgtt cggctcctcg
     2401 acacgctcaa gcgtctgcgc gacatcggca actccgt
//
LOCUS       DASURG010000532         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_90460, whole genome shotgun sequence.
ACCESSION   DASURG010000532 DASURG010000000
VERSION     DASURG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90460"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFY80_09540"
     CDS             complement(<1..346)
                     /locus_tag="VFY80_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6008195.1"
                     /translation="MAKVEMTVNGARQAREVEDRTLLVNFLRDELGLTGTHVGCDTSQ
                     CGCCTVHVNGLAVKSCTMLAVQANGADVVTIEGMAKNGALHPIQQAFTECHGLQCGFC
                     TPGMIMAVAGLLK"
     gene            complement(349..1149)
                     /locus_tag="VFY80_09545"
     CDS             complement(349..1149)
                     /locus_tag="VFY80_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6008196.1"
                     /translation="MYSFNYVKAQSLKHAGELLAANPEAKLLAGGMTLIPTLKQRLAR
                     PTHLIDLGGLKELSGIESKDGKLLIGAGVRHREVASSPAVRAAIPALAELAGVIGDPQ
                     VRNLGTVGGSVANNDPAADYPAAVLGLGATVVTTRREIPADDFFQGMFATALDESEII
                     VRLVFPIPKRAAYSKFPHPASGYAMAGVFIADTGQGIRVAVTGAAPGVFRWAAAEKAL
                     GRKFDATALEGLAVDAQTLNEDFHATREYRANLVSVMAKRALQKISGK"
     gene            complement(1159..3471)
                     /locus_tag="VFY80_09550"
     CDS             complement(1159..3471)
                     /locus_tag="VFY80_09550"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6008197.1"
                     /translation="MEHHHHLLEVEGVHVSRREDLRLITGAGKYASDWNLPGQLYACF
                     VRADRAHAKILSLDTQAVARHPGVVRIYTGEDTVRAGYVKPMTMLTFKGKGGMELKLP
                     ERPALAHGRVRCIGEPVAMVIAETALIAQDAAELVEVEYEDLPVVIDPEDALKSGAPQ
                     LHENVPGNVPFEFEAGDAAATEAAFGKAAHVTKLRLEVTRVAPNPMEPRACLMAYDAK
                     DDRYTIHVHVQGVNMMRKQLAAYTHVPEEKIRVMARDVGGAFGQRSLAYPEYCAQMLA
                     AKELERPIKWIASRSEGFATDSHGRANFVYGELALDRDGKFLGMRLDWIAEMGAYLTP
                     TGPVSHLRNPSTCLTGVYRIPALYGRWRTVLTNTNPIAAYRGAGRPDIAYVVERLVSQ
                     AAAETGTDAAELRRRNFIPPEAFPYKTPTGSTYENADLPGVLEKALKLADWNGFPARR
                     KRSEQAGQLRGIGISTVIENTGAGLFPKDTVAMDVAGDGTITVYSVSHSQGQGHETAF
                     AQIVAETLGVEFERVVLRQGDESRPLFGNHTGGSRSTVGAGSACHLAAKKLIEEGKAL
                     AALELGVEPSQVEYAKGVFMSDHAQRKLTLADLAKAKTFSVQAEGSFSSTFPNGCHIA
                     EVQIDPATGVTDIVSYVTVDDCGRVINHTIVEGQMHGGVAQGAGQVFGEHVVYDRNSG
                     QLLTGSFADYYMPRAGLLPEIRMDEHPTPSKVSPLGVKGMGESGCTASLAALTNAVLD
                     ALRPLGIQHLDMPLTPPKIWHAIQAARAKQ"
     gene            3668..>4182
                     /locus_tag="VFY80_09555"
     CDS             3668..>4182
                     /locus_tag="VFY80_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX6008198.1"
                     /translation="MKKDTLLAHAGRNPEACSGMVNTPIYRTSTVLFSTLDDYEARDP
                     NDYKSVRYGRYGTPTTFALEEAVAQMEGGHLAVAVPSGLAAAVAAICAFVKGGDHLLV
                     ADSVYGPTRQFCERRLRPVGAEIEYYDPLIGSGIARLIRSNTRAIYCESPGSLSFEMQ
                     DLPAIAACAHE"
BASE COUNT          714 a         1404 c         1320 g          744 t
ORIGIN      
        1 ctttgaggag acccgcgacg gccatgatca tgccgggggt gcagaagccg cattgcagcc
       61 catggcactc ggtgaacgcc tgctggatcg gatgcagcgc accgttcttc gccatccctt
      121 cgatcgtgac cacatcggcg ccgttcgcct gcaccgcgag catggtgcac gacttcaccg
      181 cgagcccgtt cacgtgcacc gtgcagcagc cgcactggct cgtgtcgcag ccgacgtgcg
      241 tgccggtcag ccccagctcg tcgcgcagga agttgacgag cagcgtgcgg tcctcgactt
      301 cgcgcgcctg ccgcgcgccg ttcaccgtca tttcgacttt agccatgttt atttccccga
      361 aattttctga agcgcgcgct tcgccatgac cgacaccaga ttggcgcggt actcgcgggt
      421 ggcgtggaaa tcttcgttga gcgtctgggc atcgaccgca agaccctcga gcgccgtcgc
      481 atcgaatttc cgcccgagcg ccttctccgc cgccgcccag cggaatacgc ccggagccgc
      541 gccggtcacc gccacacgta ttccctgacc cgtatcggca atgaacacac cggccatggc
      601 atagcccgaa gcgggatggg gaaactttga gtacgcggcc cgcttcggga tcgggaacac
      661 gagacgcacg atgatctcgc tctcgtcgag cgccgtggcg aacatgccct ggaaaaagtc
      721 gtcggccgga atctcgcgcc gggtggtaac gacggtcgca cccagcccga gcaccgcggc
      781 gggatagtcg gcggccggat cgttgttcgc aaccgagccg ccgaccgtgc cgaggttccg
      841 gacctgcggg tcaccgatca cccccgcaag ctctgcgagc gcgggaatcg ccgcgcgaac
      901 ggcgggggag gacgcgactt cacggtgccg cacgccggcg ccgatcagaa gctttccgtc
      961 cttggactcg atgccggaaa gctccttcag cccgccgaga tcgatgagat gcgtcggccg
     1021 cgcgagccgc tgtttgaggg tcggaatgag cgtcatgccg cccgcaagaa gcttcgcctc
     1081 gggattcgcc gcaagcaatt cacccgcgtg cttgagcgac tgcgctttca cataattgaa
     1141 ggaatacacg cgaacctcct attgctttgc ccgggccgcc tgaatggcgt gccagatctt
     1201 gggcggcgta agcggcatgt cgagatgctg gatacccagc gggcgcagcg cgtcgagcac
     1261 ggcattggtg agcgcggcga gcgatgccgt acagcccgac tcgcccatgc ccttcacgcc
     1321 gagcgggctc accttcgacg gcgtgggatg ctcgtccatc cggatctccg gcagcagccc
     1381 cgcgcgcggc atgtagtaat cggcgaagct gccggtcaga agctgacccg agttgcggtc
     1441 gtacaccacg tgctcgccga acacttggcc cgcgccttgc gcgacgccgc cgtgcatctg
     1501 accctcgaca atggtgtggt tgatgacgcg tccgcagtcg tcgacggtta cgtacgagac
     1561 gatgtcggtg acgccggtcg cagggtcgat ctgcacctcg gcgatatgac agccgttcgg
     1621 gaaggtcgag ctgaagctgc cttcggcctg caccgaaaat gtcttcgcct tggcgagatc
     1681 ggcaagggtc aacttgcgtt gcgcgtgatc gctcatgaag acgcccttcg catactcgac
     1741 ctgcgagggc tcgacgccga gctccagcgc cgcgagtgcc ttgccctcct cgatcagctt
     1801 tttagccgcc aggtggcagg cgcttcccgc gccgaccgtc gaacgcgagc cccctgtgtg
     1861 gttgccgaaa agcggtcggc tctcgtcgcc ctggcgcaag accacacgct cgaactccac
     1921 gcccagcgtt tcggcgacga tctgcgcgaa cgcggtctca tgcccttggc cctgcgaatg
     1981 cgacaccgag tagaccgtaa tcgtgccgtc gccggcaacg tccatcgcga ccgtatcctt
     2041 cggaaaaagg cctgcgccgg tgttttcgat gacggtggaa atcccgatgc cgcggagctg
     2101 ccccgcctgc tccgagcgct ttctgcgcgc ggggaaaccg ttccagtcgg caagcttgag
     2161 cgccttctcc agcacacccg gcaaatcggc gttctcgtag gtcgagcccg tcggcgtctt
     2221 atagggaaac gcttcgggcg gaatgaagtt cctgcgcctc agctcagcgg catcggttcc
     2281 ggtctccgcg gccgcctggc tcacgagccg ctcgaccaca taggcgatgt cgggacggcc
     2341 ggcgccccgg taggcggcga tgggattggt gttcgtgagc accgtacgcc aacgcccgta
     2401 aagggctgga atccgataga cacccgtaag acaggtactc ggattgcgca ggtggctcac
     2461 cgggcccgtg ggtgtgaggt aggcgcccat ctccgcgatc cagtcgagcc gcatgccgag
     2521 gaacttgccg tcgcgatcga gcgcgagctc gccgtagacg aaattcgcgc gaccgtggga
     2581 atcggtggcg aacccttccg agcgcgaggc gatccacttg atgggccgct ccagttcttt
     2641 cgcggccagc atctgcgcgc agtactccgg atacgcgaga ctgcgctggc caaacgcgcc
     2701 accgacgtcg cgcgccatga cgcgaatctt ctcttccgga acatgggtgt acgccgcaag
     2761 ctgcttgcgc atcatgttca cgccctggac gtggacgtga atggtgtagc gatcgtcctt
     2821 cgcgtcgtag gccatgaggc aggcgcgcgg ctccatcgga ttcggtgcaa cgcgcgtcac
     2881 ctccagcctg agcttcgtga cgtgagccgc cttgccgaac gctgcttcgg ttgccgcggc
     2941 gtctcctgcc tcgaactcga acggcacatt gcccggcacg ttttcgtgga gctgcggcgc
     3001 gccggacttg agcgcatcct ccggatcgat cacgaccggg agatcctcgt attcgacctc
     3061 gaccagctca gcggcatcct gcgcgatcaa cgccgtttca gcgatcacca tggcgaccgg
     3121 ctcgccgatg cagcgcaccc ggccgtgcgc gagcgccggc cgttccggca gcttgagctc
     3181 catgccgccc ttgcccttga aggtcagcat ggtcatgggc ttcacgtatc ccgcccgcac
     3241 cgtgtcttcg ccggtataga tgcggacgac gccgggatgc cgcgccacag cctgcgtatc
     3301 gagcgacagg attttcgcgt gcgcacggtc ggcgcggacg aaacaggcgt agagttgccc
     3361 cggcaggttc caatcggacg cgtacttgcc ggcgccggtg atgagccgca gatcctcccg
     3421 ccgcgagaca tgcacgcctt ccacttcaag cagatgatga tggtgttcca tgggcttccc
     3481 aatcgattat gcggaccgtt acgcgcggcg cagcctgcca aggcacggcg caatccgctt
     3541 gcgccggagt gacgcgggat tatgggtcaa ggcgcgcacc cgggcaatgc ccggagtcca
     3601 tattttgcaa cggccggccg cggggagcga ctatactgga acagactcct gaccgccttt
     3661 cgatcccatg aaaaaagata ccctgctcgc ccatgccggg cgtaatcccg aagcgtgctc
     3721 cggcatggtc aacacgccga tctatcgcac ctcgacggtc ctcttttcga cgctcgatga
     3781 ctacgaagcg cgcgacccca acgactacaa gagcgtgcgc tacggccgct atgggacgcc
     3841 caccaccttc gcgctcgaag aggcggtcgc gcagatggaa gggggtcacc tcgcggtcgc
     3901 cgtgccctcg gggctcgcgg ccgccgtagc cgcaatctgc gcctttgtga aggggggcga
     3961 ccatctgctc gtcgccgatt cggtatacgg gcctacacgc cagttctgtg agcggcggct
     4021 gcggccggtc ggcgcggaaa tcgaatacta cgatccgctg atcggatccg gcattgcgcg
     4081 gctgatcagg tcgaacaccc gagcgatcta ctgcgaatcg cccgggtcgc tcagtttcga
     4141 gatgcaggat cttccggcga tcgccgcttg cgcccacgaa ag
//
LOCUS       DASURG010000533         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1111770, whole genome shotgun sequence.
ACCESSION   DASURG010000533 DASURG010000000
VERSION     DASURG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111770"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="VFY80_09560"
     CDS             complement(<1..2073)
                     /locus_tag="VFY80_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6008199.1"
                     /translation="MDQAAREPPRTGTRVLAPGRNCWRIERAQRFRVLVDGAEYFTAL
                     RRALTRARRTIFILGWDIDSRMRLVPHGANDGFPEGLCDFLNAVVTRSRGLRAYILSW
                     DFAMLFALEREWMPVYKLDWRTHRRLSFRLDAKHPVGASHHQKVVVIDDALAFVGGFD
                     LTQRRWDTNEHAAHSPWRVDPNGEPYGPFHDIQAMVDGDVARALGELARARWQRAVGR
                     RPRAVAPRTGSNPWPEDYRADVENVDVAIARTAPPLGGSPGIYEIRQLHVDAIVAARR
                     HLFMENQYFSSSVIGNCIAQRLGEANGPEAVAIVPRRESGWLEETTMGVLRARLHQRL
                     KAADRDGRYRMYCPHLPELMNGCLNVHSKLMVADDDFLTIGSANLSNRSMGFDTECNV
                     AIESGGDARVRAAIAGIRNRVLGEHLGVDSAQVGREIEGRGSLIAAIEALRGSGRTLQ
                     PMEPALERDIDALVPDEAVIDPERPVDPDELVAEFVPPEATRPAANRMVRIAVLILFF
                     AGLAAAWRWTPLREWLDLDRLAGVADGLQAMAPFTPLAVVGAYVVAGLIVMPVTVLIA
                     VTGIVFGPFFGAVYALAGAIASAVVTYGIGRRLGRDTVRRLAGSRLNRISQQLARRGI
                     LAVALVRMLPLAPYTVVNIVAGASHISLRDFLIGTAIGMLPGILATVVFVDRIVEAMR
                     NPGVGAYIS"
     gene            complement(2210..>3113)
                     /gene="aceE"
                     /locus_tag="VFY80_09565"
     CDS             complement(2210..>3113)
                     /gene="aceE"
                     /locus_tag="VFY80_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904172.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX6008200.1"
                     /translation="MFGFQRVGDFMWAAADSRARRFLIGATAGRTTLSGEGLQHQDGT
                     SHVVAATIPNCRSYDPCFGYELAVILQDGARRMLEAQEDVFYYITVMNENYAQPPLPS
                     ASAAIEGILRGMYLVRSSPHHSTGARDASGGGSARRVQLMGSGTILREVLAAAEMLER
                     DYDVAADVWSVTSFTELARDGMAVERWNRLHPLAPPRETWVERCLKDRAGPAIAATDY
                     VRSYAEQIRGLVKRPYVVLGTDGMGRSDTRSALRAFFEVDRRHVAVAALRALAEREVL
                     PPAAAAAALSKFGIDPEVAEPWRR"
BASE COUNT          538 a         1046 c         1043 g          486 t
ORIGIN      
        1 cgaaatgtat gcgcccacgc cgggatttct catcgcttcc acgatccgat ccacgaacac
       61 gaccgttgcg agtatgccgg gtaacatgcc gatcgcggtg ccgatcagaa aatcgcgcag
      121 gctgatgtgc gatgcgccgg cgacaatgtt gacgaccgtg tacggcgcga gcggcagcat
      181 ccgcaccagc gcaacggcca agatgccgcg gcgggcgagc tgctggctga tgcgattgag
      241 acgcgaacct gcgagccgcc gcaccgtgtc gcgtcccagc cggcgcccga tgccgtaggt
      301 gaccacggcg cttgcgattg ccccagcgag ggcataaacc gcgccgaaaa acggtccgaa
      361 cacgatcccg gtcacggcga tgagtacggt gaccggcatg acgatgagac cggcgacgac
      421 gtaggcaccg acgaccgcga gcggggtgaa tggcgccatg gcctgcagcc cgtccgcaac
      481 gcccgccaga cgatcgaggt cgagccattc gcgaagcggc gtccagcgcc atgcagccgc
      541 gagtcccgcg aaaaagagta tcagcacggc gatgcgcacc atgcggtttg ccgcgggccg
      601 cgtcgcttcg ggcggcacga actccgcgac gagctcgtcg ggatccaccg ggcgctcggg
      661 atcgatgacg gcctcgtccg gaaccagtgc atcgatgtcc cgctccaggg caggctccat
      721 cggctgcaaa gtgcggcccg acccacggag cgcttcaatc gcggcgatga ggcttccccg
      781 cccttcgatt tcccggccaa cctgggcaga gtcaaccccg aggtgctcgc cgagcaccct
      841 gttgcgaatg ccggcaatcg cggcgcgcac gcgcgcatcg cctccggatt cgatcgccac
      901 gttgcactcg gtatcgaatc ccatcgagcg gttgctcaag tttgccgagc cgatggtgag
      961 gaaatcgtca tcggccacca tgagcttgct gtggacgttg aggcagccgt tcatgagctc
     1021 gggaaggtgc gggcaataca tgcgatagcg cccgtcccgg tccgccgcct tgagccgctg
     1081 gtgcaggcgc gcgcgcagca cgcccatcgt ggtttcctcg agccatccgc tttcgcgtct
     1141 cggcacgatg gcgaccgcct ccggcccgtt tgcttcgccc aaccgctgcg caatgcagtt
     1201 gccgatgacg ctcgaactga agtactgatt ttccatgaag agatgccggc gcgccgcgac
     1261 gatcgcatcc acatggagct ggcgaatctc gtaaattccg ggactgcctc cgagaggcgg
     1321 tgcagtgcgt gcaatcgcga cgtcgacgtt ctccacatcg gcacggtaat cctcgggcca
     1381 cggattgctg ccggtgcgcg gggccacggc gcgggggcgt cggccgaccg cgcgctgcca
     1441 acgcgcgcgt gccagctcgc ccagagcgcg cgcgacatct ccgtccacca tcgcctggat
     1501 atcgtgaaac ggcccatagg gctcaccgtt ggggtcgaca cgccaaggcg aatgcgcggc
     1561 atgctcgttc gtgtcccagc gtcgctgcgt gagatcgaag ccgccgacga acgccagcgc
     1621 atcgtcgatg acgacgacct tctggtgatg cgatgcgccg acgggatgct tcgcgtcgag
     1681 ccggaaactc aggcgccggt gggtgcgcca gtccagcttg tacaccggca tccattcgcg
     1741 ctcgagcgcg aacagcatgg cgaagtccca ggaaagaatg taggcgcgca gcccgcgcga
     1801 gcgggtgacg accgcgttga ggaaatcaca cagcccttcg ggaaacccgt cgttcgcgcc
     1861 gtgcgggacg agccgcatgc gactgtcgat gtcccagccc aatatgaaga tggttcggcg
     1921 cgcgcgtgtg agcgcacggc gcagcgccgt gaaatattcc gctccgtcca ccagcacgcg
     1981 aaagcgctgc gcgcgctcga tgcgccagca gtttcgcccc ggcgcgagaa cgcgcgtccc
     2041 ggtgcgtgga ggctcacgcg ctgcctggtc catcgtagat tcctgcgagg tgacggccgg
     2101 gcgcgatccc gcgccgcggc ctgtttggag cccggtcccc gaagggaccg catggccgct
     2161 tactgagaca gcggtttacc gaattggtgc ggcgccgttc ggcgcacgct cagcggcgcc
     2221 acggctcggc gacttcgggg tcgatgccga acttcgagag ggcagcggcg gccgctgcag
     2281 gcggcagcac ctcgcgctcg gcgagcgctc ggagcgccgc cacggcgacg tgacgccgat
     2341 cgacctcgaa gaacgcacga agggcggagc gcgtgtcgct gcgccccatg ccgtcggttc
     2401 ccagcaccac gtacggccgc ttgacgagcc cgcggatctg ctcggcgtag ctgcgcacat
     2461 aatcggtcgc cgcgatggcg ggtccggcgc gatctttcag gcaacgctcg acccatgtct
     2521 cgcgcggcgg cgcgagcgga tgcagtcggt tccagcgttc gaccgccatc ccatcgcgcg
     2581 cgagctcggt aaagctcgtg acactccaca cgtcggcggc aacatcgtaa tcgcgctcga
     2641 gcatttcggc tgcggcgagc acttcgcgca ggatggtgcc gctgcccatg agctggacgc
     2701 gccgggcact gccgccaccg gaggcgtccc gcgcgccggt tgaatgatga ggtgaggaac
     2761 ggacgaggta catgccgcgc agaatcccct cgatcgcggc actggcgctc ggcagaggcg
     2821 gctgtgcgta gttctcgttc atcacggtga tgtaatagaa cacgtcttcc tgcgcttcca
     2881 gcatgcgccg ggccccgtcc tgcaggatga cggcgagctc gtatccgaag cagggatcgt
     2941 acgaacggca gttgggaatg gtggctgcga ccacgtggct ggtgccgtcc tggtgctgca
     3001 agccttcgcc ggagagcgtc gtgcggcccg cggtcgcgcc gatcaagaat ctgcgtgccc
     3061 gcgagtcggc ggccgcccac atgaaatcac cgacgcgctg gaagccgaac atc
//
LOCUS       DASURG010000534         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1214336, whole genome shotgun sequence.
ACCESSION   DASURG010000534 DASURG010000000
VERSION     DASURG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214336"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFY80_09570"
     CDS             <1..589
                     /locus_tag="VFY80_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6008201.1"
                     /translation="EAANNAASAGAMVPMLALGIPGSGTTAVMLGALIMFGLRPGPQL
                     FQQHADLVWAIIASMYIGNVILLILNLPLAPLFAVLLRVPYAVLYPVILALCIVGVYS
                     QSNSIDDLWIMVVFGVLGYFMKQYDFPAAPLILGLVLEPLFENALRQSLTLSHGSLAI
                     FVTRPVSATLLVVTAAFILTPYILRLVRSQRAAAA"
     gene            730..981
                     /locus_tag="VFY80_09575"
     CDS             730..981
                     /locus_tag="VFY80_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008202.1"
                     /translation="MARISYVDPATITDPQAREWLDEAIAAGRPGPENQAIRAHQPDV
                     MRSFTLTRKMLFDNGAGVLEHDLKELVRAYIAHTVECGY"
     gene            1027..1290
                     /locus_tag="VFY80_09580"
     CDS             1027..1290
                     /locus_tag="VFY80_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008203.1"
                     /translation="MSELMRYAKSERFTSREKLALRYADAIMYDPAQADDALWAELHA
                     EFSEPQLVELGYWIGFTFGGQRWLKTLDAKQGELAAFLRSEGR"
     gene            1510..2274
                     /locus_tag="VFY80_09585"
     CDS             1510..2274
                     /locus_tag="VFY80_09585"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX6008204.1"
                     /translation="MPELPARTHPFDPGYDPHTVLSHIEQSAHLMASLKISMACWLVG
                     GEDAYRRKIAFARRHGVTTVTGGGPFEIAVELDKLDHYLALCADMGIDRIEAGQGFTA
                     MPLAPAEVARRAADFGLGLQFELGKKHGGSFGSDTVGELVEQGRAWLDAGAVYLVIEG
                     RESAAGVGIFDDSGRLNCAAADRFAAAFGLDRVVFEAPNKASQFALMRHFGERVALSN
                     VRLEELLRVEIYRRGLHSDAFDADVFRRRMASAPHG"
     gene            2310..3539
                     /locus_tag="VFY80_09590"
     CDS             2310..3539
                     /locus_tag="VFY80_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008205.1"
                     /translation="MSEPLDTSVPVLVLKMHHGSLGIARSLGRLGIAVHGLAARRELW
                     AARSRYWRGCYQWDVENAPAQRSIDYLLALGKRLGRLPLLIPVSDRTAEFVAEHAEVL
                     RTAFRFPNLSHDLVTRLTSKKAMYFVARRCGIPTPDTLFPASRKDVIDSIDRIRFPVM
                     LKGIDGLRLHARTGHTMAIVRNASELLDHYDRMEDPAHPNLMLQEYIPGGEDSVWMFN
                     GYFDAESRCLIGFTGQKIRQTPVYTGSTSLGIVRNNDNVAATTCAFMKAIGYRGILDI
                     GYRYDARDGEYKVLDVNPRIGSTFRLFVGANGMDVVRALYRDLTGQSVPSSLSVEGRK
                     WIVEDQDIISSYRYWRDGNLGVIQWLASLRGIREGAWFAWDDRAPFLHMCRSLMKSGA
                     RWVTRKLMFTAPRKAAS"
     gene            3536..>3812
                     /locus_tag="VFY80_09595"
     CDS             3536..>3812
                     /locus_tag="VFY80_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008206.1"
                     /translation="MRASEATPPLRALKRALASFVITALPLALRQSGAAWIGRQPWIP
                     GRHWRAARLVADLAERDPNAYHRFLWSHHLAYAESYEPSLQYGRKHIK"
BASE COUNT          672 a         1210 c         1186 g          744 t
ORIGIN      
        1 ggaggcggcc aacaatgcgg catcggccgg cgccatggtc cctatgttag ccctcgggat
       61 cccgggatca ggcacgaccg ctgtcatgct gggcgcgctc atcatgttcg ggctgcgtcc
      121 cggccctcag ctttttcagc aacacgctga tctggtgtgg gcgatcatcg cgagcatgta
      181 tatcggtaac gtgatactcc tgatcctcaa cttgccgctt gcgccgctct tcgcggtatt
      241 gttgcgagtg ccgtatgcgg tgctgtaccc cgtgatcttg gccctatgca ttgtgggcgt
      301 ctacagtcag tcgaacagca tcgatgatct atggatcatg gtcgtcttcg gcgtgcttgg
      361 ttactttatg aaacagtacg atttcccggc cgcgccgctg attctcggcc tcgtcctcga
      421 gccgcttttt gagaatgcac ttaggcagtc ccttacgctc tcgcatggca gtctcgcaat
      481 ctttgtcacc cgccccgttt ccgccacgct actcgtcgtc acagctgcct ttatcctcac
      541 tccctatatt ctgcggctag ttcgatcgca gcgcgctgcg gctgcttaac cttgccgccc
      601 acttcgtttg acgccgcctt acgccggatt ttggcgttat atcgctaaaa tactctccga
      661 taagcaaaac gaaccgtcgg tgaaggccat gccgatcgat ggggcgagac attacgcgga
      721 gaaaacctga tggcacgcat aagctacgtc gatccggcaa cgatcaccga tccgcaggcg
      781 cgcgagtggc tcgacgaggc gatcgcggcg ggccgccccg ggcccgagaa ccaggcgatc
      841 cgcgcgcatc agccggacgt catgcgctcc ttcacgctca cgcgcaagat gctcttcgac
      901 aatggcgccg gcgtactcga gcacgatctt aaggagctcg tgcgggccta catcgctcac
      961 accgtcgaat gcggttactg aagcaaccag ggaaccgcgc ggtcggcgcg ggacagtgaa
     1021 agccgcatga gcgaacttat gcggtacgcg aagtccgagc gcttcacttc gcgggaaaag
     1081 ctagcgctgc gctacgccga cgccatcatg tatgaccccg cccaggccga cgacgcgctg
     1141 tgggccgagc tgcacgcgga attctccgaa cctcaactgg tcgagctcgg ctactggatt
     1201 ggcttcacgt tcggcggaca gcgctggctg aagaccctgg atgcgaagca gggcgagctc
     1261 gcagcgttcc tgcgcagcga gggccgataa ccgctgtcgc gccttgccta agccgctgat
     1321 tcggctcgga cccgcaaatg cagcaagaat caagccaata gcgctgcgcc gtgagaacca
     1381 cccatcccca cgaacgggta tgccgttgtg ctccaagaat gacggcatca cgggtggagg
     1441 tgtgccatca gcatcaaacc gctcaagaaa ccgacgtgga gcacggctgc cttcctgcgc
     1501 gagctggggg tgccggagct gcctgcgcgc acgcatccgt tcgaccccgg ctacgatccg
     1561 cacacggtcc tgagccacat cgagcaatcg gcgcacctca tggcgagctt gaagatctcc
     1621 atggcgtgct ggctggtcgg tggggaagac gcctatcgcc ggaagatcgc ctttgcgcgc
     1681 cggcacggcg tcaccacggt gaccggaggc ggcccgttcg agattgcggt cgaactcgac
     1741 aagctcgacc attacctcgc gctctgtgcc gacatgggca tcgaccgcat cgaggcgggg
     1801 cagggcttca ccgccatgcc gcttgcgcct gcggaagtgg cgcgtcgtgc cgcggacttc
     1861 ggcctcggcc tgcagttcga gctcggtaag aagcacggtg gcagcttcgg ttccgatacg
     1921 gtcggtgaac tcgtcgagca gggcagggcg tggcttgatg caggcgccgt ctatctcgtg
     1981 atcgagggac gtgaaagcgc ggcaggcgtc ggcatcttcg atgactcagg ccggttgaac
     2041 tgcgcggcgg ccgatcgttt cgctgcggcc ttcggtctcg atcgggtcgt cttcgaagcc
     2101 cccaacaagg cgagccagtt tgcgctgatg cggcatttcg gcgagcgcgt cgcattgtcc
     2161 aacgtgcggc tcgaggaact gctccgcgtg gagatctacc gcagaggctt gcattctgac
     2221 gcattcgacg ccgatgtgtt ccgcaggcgc atggcgtctg cgccgcacgg ttgatcgccc
     2281 gcctcgacat ccgcacctcg ggcggcacga tgagcgaacc tctcgatacg tccgttccgg
     2341 tgcttgtgct caagatgcat catggcagcc tggggattgc ccgcagcctg gggcggctcg
     2401 gcatcgcggt gcatgggctc gctgcgcgac gtgagctgtg ggcggcacgc tcgcgttact
     2461 ggaggggatg ctatcagtgg gacgtcgaga acgcgccggc acagcgctcg atcgactatt
     2521 tgctcgcgct cggtaagcgg ctggggcgcc tgccgctcct cattcccgta tcggaccgca
     2581 cggcggagtt cgtggcggaa catgctgaag tcctgcgcac cgcattccgc tttccaaacc
     2641 tctcgcacga tctcgtaaca aggctcacga gcaagaaggc gatgtacttt gtcgcgagac
     2701 ggtgcggtat tccgacgccc gacacactgt tccctgcatc acggaaggac gtcatcgatt
     2761 ccatcgaccg cattcgcttt cccgtgatgc tgaagggcat cgacggattg cggctgcatg
     2821 cgcgtaccgg tcacacgatg gcgatcgtaa gaaacgcgtc ggagcttctc gaccattacg
     2881 accgcatgga agacccggcc catcccaatc tgatgctgca ggagtacatc ccgggcgggg
     2941 aggacagcgt ctggatgttc aacggctatt tcgatgcgga aagccgctgt ctcatcggat
     3001 tcaccggaca gaagatccgg cagacacccg tctacacggg ctcgacatca ctgggcatcg
     3061 tgcgcaataa cgacaacgtt gcggcgacca cgtgcgcctt catgaaggcg attggatacc
     3121 gcggcatcct cgacatcggc taccgctacg atgcgcgcga cggcgagtac aaggtgctcg
     3181 acgtgaaccc tcgcatcgga tcgacgttcc ggcttttcgt cggcgcgaac gggatggatg
     3241 tcgtgcgcgc gctctaccgc gacctgaccg ggcagagcgt gccgtcgtct ctttcggtag
     3301 agggccgcaa atggatcgtc gaggatcagg acatcatctc gtcgtaccgc tattggcgcg
     3361 atgggaatct gggtgtgatc cagtggctcg cttcgctgcg cgggatacgc gagggcgcat
     3421 ggttcgcgtg ggatgaccgg gcgccctttc tccacatgtg ccgcagtctc atgaagagcg
     3481 gcgcacgctg ggtgacccgc aagctcatgt tcaccgctcc gcgcaaggcg gcgtcatgag
     3541 ggcttcggag gctactccgc cgcttcgcgc gctcaagcgg gcgctcgcct cgttcgtcat
     3601 cacggcgctc ccgcttgcgc ttcgccagtc tggagcagcg tggatcggac gtcagccctg
     3661 gattcccggc cggcactggc gcgcagcgcg cctcgtcgcc gatctcgccg agcgcgatcc
     3721 gaacgcctac caccgttttc tgtggtcgca tcacctggca tatgccgagt cgtacgagcc
     3781 ttcgctgcag tacgggcgca aacacatcaa gc
//
LOCUS       DASURG010000535         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_499337, whole genome shotgun sequence.
ACCESSION   DASURG010000535 DASURG010000000
VERSION     DASURG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499337"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFY80_09600"
     CDS             <1..485
                     /locus_tag="VFY80_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HEX6008207.1"
                     /translation="RVKPDLADRMGLTGLAGRASAVAWDLRVQFPPAPYDQFDVRMVT
                     HRNGDVAARVTVRFEEIAESLRLIDLILSGLPEGEITVPLAEAPEDAFGAGWVEGWRG
                     EVLIALETGRGSRIRRLHPHDPSWQNWPLLERAVLGNIVPDFPLINKSFNLSYSGQDL
                     "
     gene            493..1008
                     /locus_tag="VFY80_09605"
     CDS             493..1008
                     /locus_tag="VFY80_09605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX6008208.1"
                     /translation="MYQLLKQIVATGIRTEAPPEPDEALRVTGERLRTEVLERFAGSL
                     TIRHVDAGSCNGCELEINALNNPYYNLEGLGIRFVASPRHADLLLVTGPVSRNMQTAL
                     KRTYDATPEPKLVVALGDCGCTGGIFGESYASCGRVSNVIPVDVAVPGCPPPPLAIMQ
                     GILTAIATVRR"
     gene            complement(1034..1864)
                     /locus_tag="VFY80_09610"
     CDS             complement(1034..1864)
                     /locus_tag="VFY80_09610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6008209.1"
                     /translation="MLALATPSRAEYEVHWVSFPALKPFEGEAVELSALEFRPKSGGP
                     FPAIVMLHGCSGMFTPSGYITNSYRNWAELLAEDGYVALLVDSFNPRGHRSICEQQKR
                     AILESRERVEDAYAAAGWLQAQPYVANDRIGLIGWSNGGTGALYSMRPASRMEPAFRA
                     AVAFYPGCRTLSRAKTLYRPYAPLLILTGEADDWTPAEPCKQLAALAGKESGALEIVT
                     YPGAHHSFDRVGLPVRYRPNVRNLNKPDRLGATVGEHPQAREDAVRRTKAFFAQSLKN
                     "
     gene            complement(1985..>2969)
                     /locus_tag="VFY80_09615"
     CDS             complement(1985..>2969)
                     /locus_tag="VFY80_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6008210.1"
                     /translation="TSEVYNGDNREQGPEIVRLIENELAPLVKGMSVFEHERVWEKMF
                     QLTHTSRDRKVLLEAIACVDCALWDLVGKALGKSVCALLGGYRGALPIISIGGYYMPG
                     KTLADIGREMDMYREAGMAGCKFKVGGLTPEEDAKRVRAAREAAGAGFILMVDANRGW
                     NAQDAIRFARLVENCDIRWFEEPCHWYDDAALMAQVRQSISIPIAAGQSEITSHGVRR
                     LVDARAVDVVNYDASEGGGVSDWRRAAALCATAGIEMAHHEEPQIAQHLLAGVPHGTY
                     LECFADPDRDPAWQALWANRPPVRDGRVEVARGPGFGIELDAAMIRKYRVN"
BASE COUNT          483 a          987 c          986 g          513 t
ORIGIN      
        1 ggcgcgtgaa acccgacctc gccgaccgca tgggtctcac gggcctggcg ggacgggcga
       61 gcgcggtcgc atgggacctg cgcgtgcagt tcccgcccgc gccgtatgac cagttcgatg
      121 tccgcatggt gacgcatcgg aatggcgacg tggcggcccg ggtgacggtg cgcttcgagg
      181 aaatcgcgga gtcgctgcgc ctgatcgacc tcatcctctc aggcctgccc gagggggaaa
      241 tcaccgtgcc gctcgcggaa gcgccggagg atgccttcgg cgcggggtgg gtggaaggct
      301 ggcgcggcga ggtgctgatc gcgctcgaaa ccggtcgggg gagccgcatc cggcggcttc
      361 atcctcacga cccgtcatgg cagaactggc cgctcctcga gcgcgcggtg ctgggcaaca
      421 tcgttcctga cttcccgctc atcaacaaat ccttcaacct gtcctacagc gggcaggatt
      481 tatagtggcg ccatgtatca actgctgaaa cagatcgtcg caaccggcat ccgcaccgag
      541 gccccgcccg agcccgatga agcgttgcgt gtgaccggag agcggctgcg cacggaagtg
      601 ctcgagcgct ttgccggctc gctcacgatc cgccacgtgg atgccggctc gtgcaacggc
      661 tgcgagctgg agatcaacgc gctcaacaac ccctactaca atctcgaagg gctaggcatc
      721 cggttcgtcg cgagccctcg ccatgcggac ctgctgctcg tcacggggcc ggtatcgcgc
      781 aacatgcaaa cggcgcttaa gcgcacctat gacgcgacgc cggagcccaa gctcgtcgtc
      841 gcactcggcg attgcggctg taccggcggt atctttggcg agagctacgc aagctgcggg
      901 cgcgtttcca acgtgattcc ggtggatgtc gcggtgcctg ggtgtccgcc gccgccgctc
      961 gccatcatgc agggcattct caccgcgatc gcgaccgtca ggcgttgatc tgccggcgcg
     1021 cccggctgtg cggtcagttt ttcagtgatt gggcaaagaa cgccttggtg cgccgaacgg
     1081 catcttcgcg tgcctgcggg tgctcgccaa cggtcgctcc gagccggtcg ggcttgttga
     1141 ggttccttac gttggggcgg tagcgcacgg gcagccctac gcgatcgaag ctgtggtgcg
     1201 cgccaggata ggtgacgatc tcgagcgcgc cactttcctt ccccgcgagt gccgcgagct
     1261 gcttgcacgg ctccgccggc gtccagtcgt cggcctcgcc ggtcaggatg agcaggggcg
     1321 cgtaaggacg gtagagcgtt ttcgcgcgcg acagcgtccg gcacccggga tagaaggcaa
     1381 cggcggcgcg gaaggccggt tccatgcggc ttgcggggcg catgctatag agggccccgg
     1441 tgccgccgtt cgaccagccg atgagcccga tgcggtcgtt cgcgacgtac ggctgcgcct
     1501 gaagccagcc cgccgcggca taggcgtcct cgacccgctc gcggctctcg aggatcgcgc
     1561 gcttctgctg ctcgcagatc gagcggtgcc cgcgtggatt gaaactgtcc accagcaggg
     1621 cgacatagcc gtcttccgcg agcagttcgg cccagttccg gtatgagttc gtgatgtagc
     1681 ccgacggcgt gaacattccg ctgcagccgt gaagcatgac gatcgcaggg aagggaccgc
     1741 ctgacttcgg ccggaattcc agcgccgaca gctcgaccgc ttcgccttcg aagggcttca
     1801 gggccgggaa cgaaacccag tgcacctcgt actcggcgcg cgaaggcgtg gcgagcgcca
     1861 gcatcgacgc cacgatggtg gcggcacaga agcgaagaag cgcgtgccag agcgcacgtg
     1921 actggattgc agcttgcgcc ggaacgacgg tgccttgaac gggcggaatg acgatgcgct
     1981 cctcttagtt gacgcgatac ttgcgaatca tggcggcgtc gagctcgatg ccgaatcccg
     2041 gcccgcgcgc gacttcgacc ctcccgtcgc gcaccggcgg gcggttcgcc cacagcgcct
     2101 gccatgccgg atcgcgatcg ggatccgcaa agcactccag atacgttccg tggggcacgc
     2161 cggcgagcag atgctgagcg atctgcggct cttcgtggtg tgccatctcg attccggcag
     2221 ttgcgcacag cgccgcagcg cgacgccagt cgctgacacc tccgccttcg gacgcatcgt
     2281 agttgacgac gtccacggcg cgcgcgtcga caagccgtcg cacgccgtgg ctcgtgatct
     2341 cggactggcc ggcagcgatc ggaatggaga tcgactgcct gacctgcgcc atcaacgccg
     2401 cgtcgtcgta ccagtggcag ggttcctcga accagcgaat gtcgcagttc tccacgagac
     2461 gagcgaagcg aatggcatcc tgcgcgttcc agccgcggtt cgcgtccacc atgaggatga
     2521 agcccgcgcc ggcggcttcg cgggcagcac gcacgcgttt cgcgtcctcc tccggggtca
     2581 agccgccgac cttgaacttg cagcccgcca tgccggcttc gcggtacata tccatctcgc
     2641 ggccaatgtc ggcgagcgtc ttgcccggca tgtaataacc gccgatcgaa atgatcggca
     2701 gcgcgccgcg atagccgccg aggagtgcgc acacgctctt gccgagcgct ttcccgacga
     2761 gatcccacag cgcgcagtcg acgcaggcga tcgcttcaag gagcaccttg cggtcgcggc
     2821 tcgtgtgcgt caactgaaac atcttctccc agacgcgctc gtgctcgaag acgctcatgc
     2881 ccttcaccag tggcgcgagt tcgttctcga tcaggcgcac gatctccggg ccctgttcgc
     2941 ggttgtcgcc gttgtagacc tcgctcgtg
//
LOCUS       DASURG010000536         4864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_907810, whole genome shotgun sequence.
ACCESSION   DASURG010000536 DASURG010000000
VERSION     DASURG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907810"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VFY80_09620"
     CDS             <1..1448
                     /locus_tag="VFY80_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX6008211.1"
                     /translation="REATIATCEPHIREWEEPLKQVLRLDIKRVVANPLDIRSYLVEF
                     YNLARSVKGAASQDKGAYSEIANFEQLVQLGRSGKLDANDQHIVHICDWLFNYAFQER
                     ASDIHLEPRREVGNVRFRIDGMLHQVYQIPTPVMAAMTSRIKILGRMDVVEKRRPQDG
                     RLKTITPDGEEVELRLSTMPTAFGEKLVMRIFNPENLVKDYHELGFTETEEAQWRQMV
                     GQPHGIILVTGPTGSGKTTTLYSTLKQLAVPEVNVCTVEDPIEMVEPAFNQMQVQHGI
                     GVDFAAGIRTLMRQDPDIIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNDAPSAITR
                     LLDIGVPPYLLHGTILGVMAQRLVRTLCPHCKEKHELDDSAWDTLVSPWKAAKPRDTT
                     YIAKGCLECRMTGYLGRVGIYETMVMTGELRKLVTQNTDLDELREKAYKDGMKPLRIT
                     GAMKVAAGLTTIDEVIAVAPPPSGDRRQHPR"
     gene            1550..2443
                     /locus_tag="VFY80_09625"
     CDS             1550..2443
                     /locus_tag="VFY80_09625"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6008212.1"
                     /translation="MNNASFATDQTSRDFSRIARAVEFLQREAPRQPDLSAVARHVNV
                     SEFHLQRLFTHWAGVSPKRFLQYLTVEHAKGRLADSRSVVDVADRVGLSGPGRLHDLF
                     VTIEAMSPGEFRAAGAGITLSYGVHPSPFGACLIATTARGICTIHFMQDGEDATRFIR
                     DAWPRARCIWDPVATGAIAATLFEPLALRHAPLALLVKGTNFQLQVWRALLAVPPGRL
                     TSYSEIARRIGRPHSQRAVGNAVAANPIAWLIPCHRVIRETGRFGDYRWGVARKAAML
                     GWEAARDEAAGLSRTLASTAP"
     gene            2440..2871
                     /locus_tag="VFY80_09630"
     CDS             2440..2871
                     /locus_tag="VFY80_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6008213.1"
                     /translation="MKASLQPGLVYRFQFTIPPTKTVPHLYPESEMFREMPEVLATGY
                     LVGLMEWACIEALRPHLDWPREQSLGTQVNFTHLAPTPPGFTVTVNVRLENVDGRKLT
                     FALSAHDGVDLISEGTHERVVIDAARFKSKMEQKSETPSWR"
     gene            complement(2911..4092)
                     /locus_tag="VFY80_09635"
     CDS             complement(2911..4092)
                     /locus_tag="VFY80_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6008214.1"
                     /translation="MLIEFFLRLKEGGVPVSIREFLTLLAALEKRVIFGSFEDFYHLA
                     RTCLVKDEKYFDRYDRVFSAYFRGITDVESIAVVIPEEWLVKMAEKFLTEEEKKQIEA
                     LGGWDKLMETLRKRLEEQNGRHQGGGKWIGTAGTSPFGAYGYNPEGVRIGQDESRHRR
                     AVKVWDKREFRNLDDDVEIGTRNIKVALRRLRRFAREGNPEELDLDDTIRSTAKNAGW
                     LDLKMVPERHNAVKVLLFLDVGGSMDDHVVLCEQLFSAARSEFKRLEYFYFHNFLYER
                     VWRDNHRRATERISTWDVLHTYPHDYKVIFVGDATMSPYEIVYPGGSVEHNNEEPGAV
                     WMQRLLAVYPNAIWLNPQPESVWDYHESIRITRELLGERMYPLTLEGLDRGMRVLTKS
                     H"
     gene            4244..4516
                     /locus_tag="VFY80_09640"
     CDS             4244..4516
                     /locus_tag="VFY80_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008215.1"
                     /translation="MTKRLGLITAGAALAIASGSAMAGGDVSFGISIGVPVAPAPVYV
                     APPPVVYAPPPRVYYAPATVYYAPAYYAPVVRYKPKHWHKHHYRHW"
     gene            complement(4583..>4864)
                     /locus_tag="VFY80_09645"
     CDS             complement(4583..>4864)
                     /locus_tag="VFY80_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6008216.1"
                     /translation="IVEVHFPGIKQRLVAEALRLFYEVREVPGLKKKPSTSELLDWLK
                     LLMAEDIGPETLRERDPKKLIPPLHGALLKNEQDVHLFERLVFMSRRKL"
BASE COUNT          925 a         1586 c         1457 g          896 t
ORIGIN      
        1 cgcgcgaagc gaccatcgcc acctgcgagc cccacatccg cgagtgggaa gagccgttga
       61 agcaggtgct gcggctcgac atcaagcgcg tcgtcgccaa cccgcttgac atccgcagct
      121 atctcgtcga gttctacaac ctcgcccgtt ccgtgaaagg cgctgcatcg caggacaagg
      181 gcgcatacag cgagatcgcg aacttcgagc agctggtgca acttgggcgc agcggcaagc
      241 tcgacgccaa tgaccagcac atcgtccata tctgcgactg gctgttcaat tacgcgttcc
      301 aggagcgcgc gagcgatatt catctcgagc cgcggcgcga agtgggcaac gtccgtttcc
      361 gcatcgacgg gatgctgcat caggtctacc agattccgac gcccgtcatg gctgcgatga
      421 cgagtcgcat caaaatcctc ggacgcatgg acgtcgtgga aaaacgccga ccccaggacg
      481 ggcggctgaa gaccatcacg cccgacggcg aggaagtgga actgcggctc tccaccatgc
      541 cgacggcgtt cggtgagaag ctcgtcatgc ggatcttcaa ccccgaaaac cttgtgaagg
      601 actatcacga gctcgggttc acggagaccg aggaggcgca gtggcggcag atggtcggcc
      661 agccgcacgg catcatcctc gtcaccggtc cgaccggatc gggcaagacg accacgctct
      721 attcgacgct caagcagctc gccgtgccgg aagtgaacgt ctgcacggtc gaggatccga
      781 tcgaaatggt cgagccggcg ttcaaccaga tgcaggttca gcacggcatc ggcgtcgatt
      841 tcgcagccgg gatccgtacc ctcatgcgcc aggatccgga catcatcatg gtcggcgaga
      901 ttcgcgatct ggaaaccgcc gaaatggccg tccaggcggc gcttaccggg cacctcgtgc
      961 tctcgacgct gcacacgaac gatgcgccgt cggcgatcac acggctcctc gatattggcg
     1021 tgccaccgta tctcctgcat ggcacgatcc tcggcgtgat ggcccagcgc ttggtgcgca
     1081 ccctctgccc gcactgcaag gaaaagcacg agctggacga ctcggcgtgg gatacgctcg
     1141 tctccccctg gaaggccgcg aagccgcgcg atacgaccta catcgccaag gggtgcctcg
     1201 aatgccgcat gacgggctat ctcggccgcg tcggcatcta cgagacgatg gtgatgacgg
     1261 gggaactgcg caagctcgtc acccagaata cggacctcga cgaattgcgc gaaaaagcct
     1321 acaaggacgg gatgaaaccg ttgcgcatca ccggcgcaat gaaggttgcg gccggtctca
     1381 ccacgatcga cgaagtgatc gccgtcgccc cgccgcccag cggcgaccgc cgccagcatc
     1441 cgcgctagag aagtcgtcct cgcgcccggg atgatgcaag gcgtcgcgtc atcgaactca
     1501 gcaagaaacg ggtagcgcgt tcctcacccc cggcttatcc tgaatcgcca tgaataacgc
     1561 atcctttgcc acagaccaaa cctcacgcga cttcagccgc atcgcgcgcg ccgtcgagtt
     1621 cctgcagcgt gaagcgcctc gacagcccga cctttcggcc gtcgcccggc acgtcaacgt
     1681 gagcgagttt cacctgcaaa ggctctttac gcactgggcg ggcgtgagcc cgaagcgatt
     1741 tctgcaatac ctcaccgtgg agcacgctaa gggccggctt gccgattcgc gcagcgtcgt
     1801 cgatgttgcg gatcgggtgg ggctttccgg gccggggcgg ctgcatgatc tcttcgtcac
     1861 catcgaagcg atgtcaccgg gcgaattccg tgcggcgggc gccgggataa cgctaagtta
     1921 cggcgtgcat ccgtcgccat tcggcgcctg cctgatcgcg acgacggcac gcggcatttg
     1981 cacgatccat ttcatgcagg acggtgaaga tgcgactcgc ttcattcggg acgcgtggcc
     2041 gcgcgcacgg tgcatatggg atcccgtggc gacaggagcg atcgccgcga cgctcttcga
     2101 gccgcttgcg ctacggcacg caccgcttgc gttgctcgtc aagggcacca acttccagct
     2161 ccaggtgtgg cgcgcgctcc tcgccgtgcc gcctgggcgc ctcacgagct acagcgagat
     2221 cgcgcgacgc atcgggcgtc cccactcgca acgcgcggtc ggaaacgccg ttgcggcaaa
     2281 ccccatcgca tggctcattc cctgccaccg cgtcatccgc gagaccggcc ggttcggcga
     2341 ctaccgctgg ggcgttgcgc gcaaagcggc gatgctcggg tgggaagccg cgcgtgacga
     2401 agccgcgggg ctttcgcgca cgctcgcctc gactgcgcca tgaaagcatc cctgcaaccc
     2461 gggctcgttt accgcttcca gtttacgatc cccccgacca agaccgtgcc gcacctctac
     2521 ccggagtcgg agatgttccg cgagatgccg gaggtgctcg ccacgggata cctcgtcggg
     2581 ctcatggagt gggcgtgcat cgaagcgctc cgcccgcatc tcgactggcc ccgcgagcag
     2641 tcgctcggca cgcaggtgaa tttcacgcac ctcgccccca cgccgccagg atttaccgtg
     2701 acggtcaacg tccgcctcga gaacgtcgat ggccggaaac tcaccttcgc gctgtccgct
     2761 cacgacggag tcgacctcat ctccgaggga acacacgagc gcgtcgtgat cgacgccgcg
     2821 cgcttcaaat ccaaaatgga gcagaaatca gaaacgcctt cctggcgata aggcgcatgg
     2881 atttcggtgt tcgtcggaat gagaagctgc ctagtgcgac ttcgtgagca cacgcatgcc
     2941 gcggtcgagg ccctcgagcg tcagcggata catgcgctcg cccaggagct cgcgcgtgat
     3001 gcggatcgat tcgtgatagt cccagaccga ctcgggctgc ggattgagcc agatcgcgtt
     3061 gggataaacc gccagtagac gctgcatcca caccgcgccg ggctcctcgt tgttgtgctc
     3121 gacgctcccg ccggggtaaa cgatttcgta ggggctcatc gtcgcgtccc ccacgaagat
     3181 caccttgtag tcgtgcggat acgtgtgcaa cacgtcccag gtcgagatgc gctccgtcgc
     3241 gcggcggtga ttgtcgcgcc agacgcgctc gtagagaaaa ttgtggaaat agaagtactc
     3301 gaggcgcttg aattcgctgc gtgcggcgga gaaaagctgc tcacagagga cgacgtggtc
     3361 gtccattgat ccaccgacat cgagaaagag cagcaccttc accgcattgt gccgttcagg
     3421 caccattttt aaatcgagcc agccggcgtt ctttgcggtg gagcggatgg tatcgtctag
     3481 atcgagttct tccggattgc cttcgcgcgc gaagcgccgc agccgccgca aggcgacctt
     3541 gatgttgcgc gttccgatct ctacatcgtc atccagattg cggaattcgc gcttgtccca
     3601 gaccttcacc gcgcgccggt ggcgcgattc atcctgaccg atccgcacgc cttcggggtt
     3661 atatccgtac gccccgaacg gagacgtgcc ggccgttccg atccatttgc cgcccccctg
     3721 gtgccgtccg ttctgctctt ccaggcgctt gcgcagcgtc tccatcagct tgtcccagcc
     3781 gccgagcgcc tcgatctgct tcttctcttc ctccgtgagg aatttttccg ccatcttcac
     3841 tagccattcc tccggaatga ccactgcgat gctctcgacg tcggtaatgc ccctgaaata
     3901 ggcggagaac acccggtcgt agcggtcgaa gtatttttcg tcctttacga ggcaggtgcg
     3961 cgcgagatgg tagaagtcct cgaagctgcc gaagatcacg cgcttttcca gcgccgcgag
     4021 cagcgtcaga aactcgcgga tcgagaccgg cacgccccct tctttcagtc gcaggaagaa
     4081 ctcaatcagc atgggcttga acatttccct ttttgactca aagggataag cagcctcttt
     4141 gccatttaca agccgttaca atttctgaca gattttaaga gcacgttgcg tcatcatgca
     4201 gccgtgcggg tcgatgaccc ggaccagaac aaggaggcat gccatgacca aacgtctcgg
     4261 tctcattaca gcgggtgcag ccctcgcgat tgccagcggc agcgcaatgg cgggcggcga
     4321 tgtgagcttc ggcatatcga tcggtgtacc ggtcgcgcca gcccccgtgt acgtcgctcc
     4381 gccgccggtc gtgtacgcgc cgcctccccg cgtctactat gcgccggcca cggtgtacta
     4441 cgcgcccgcc tattacgcgc cggtcgtgag gtacaagccc aagcactggc acaagcatca
     4501 ttaccgccac tggtaaagcg ggaaattaga cgtcccggga aaagcgcgcc atcggcgcgc
     4561 ttttcttttg ggcgttggcg cgtcacagct tgcggcgcga catgaacacg agccgctcga
     4621 aaaggtgcac gtcctgctcg ttcttcaaga gcgcgccgtg cagcggcgga atgagcttct
     4681 tgggatcgcg ctcgcgcagg gtttccgggc cgatgtcttc ggccatcagc agcttgagcc
     4741 agtcgagcaa ttcggaggtc gagggctttt tcttcaatcc gggaacttcg cgaacctcgt
     4801 agaacagccg cagcgcctcc gccaccagcc gctgcttgat gcccggaaaa tgcacctcga
     4861 cgat
//
LOCUS       DASURG010000537         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_704027, whole genome shotgun sequence.
ACCESSION   DASURG010000537 DASURG010000000
VERSION     DASURG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704027"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFY80_09650"
     CDS             <1..578
                     /locus_tag="VFY80_09650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX6008217.1"
                     /translation="LREKYPNIPLYAVVEDICASGGYYVAVAADQIYVDKSSIIGSIG
                     VLMDGFGFTGLMDKLGVERRLLAAGENKGFLDPFSPIEESQKAHAQNMLNEIHDQFIN
                     VVRQGRGNRLKENPEMFSGLLWIGTKSVELGLADGLGSVEYVAREVIKAEDIVDFTPR
                     ENIAERVARRFGAAMAETLFKLAGLSAYALR"
     gene            complement(541..1287)
                     /locus_tag="VFY80_09655"
     CDS             complement(541..1287)
                     /locus_tag="VFY80_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6008218.1"
                     /translation="MQQPGTLYLVPTGLGGDPGDVLPPKTRERLLALTVFIVENAKSA
                     RQFLKAAGYPHALPTTEMHVLDEHTRASAMTQLLAPLLAGRDCGLLSEAGAPAVADPG
                     ALLVRLAHQTGIRVVPLVGPSAVLLALMASGLNGQRFDFHGYLPVRGEERATRLKELE
                     HASQRRDSTQIFIEAPYRNDSLMRAILESCRDDTLLCIAADLSLPSEFIRTQPIAAWR
                     KTPPHLDRRPAVFLLYCSLSAARRPTVRPA"
     gene            complement(1302..1880)
                     /locus_tag="VFY80_09660"
     CDS             complement(1302..1880)
                     /locus_tag="VFY80_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX6008219.1"
                     /translation="MHTRLVLASSSPYRRALLERLGLPFEVIVPSVDERELPGETAER
                     MALRLAESKARAVSPSNAALVIGSDQVAEVEGVKLGKPGDHENAVRQLRQVRGKRIVF
                     HTAICLANTASGRMQLADVTTAVQFRPYTDEQIERYLVRERPYDCTGSARIEGLGIAL
                     IETVSSDDPTALIGLPLMQLVTMLANEGINAL"
     gene            2008..2520
                     /locus_tag="VFY80_09665"
     CDS             2008..2520
                     /locus_tag="VFY80_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX6008220.1"
                     /translation="MSAQTVIDSLEFARAAQQLQGSVPVASLTRLQDCLSDNGGEVRF
                     RVYGARDEERRPVLEVEISGMLHLQCQRCLGPLDYPLHVRNTLLLVRPGENPGPDDDD
                     PEAPDYIEASAEQDVAALIEDEILLSLPFAPRHPESTCRGEWGAGERSGHKESAFARL
                     AELRNSGHKE"
     gene            2537..2716
                     /gene="rpmF"
                     /locus_tag="VFY80_09670"
     CDS             2537..2716
                     /gene="rpmF"
                     /locus_tag="VFY80_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX6008221.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDSLTNPPLAVEPTTGEVHLRHHISP
                     NGYYRGKKVIRNKGE"
     gene            2804..3820
                     /gene="plsX"
                     /locus_tag="VFY80_09675"
     CDS             2804..3820
                     /gene="plsX"
                     /locus_tag="VFY80_09675"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX6008222.1"
                     /translation="MDVTVAIDCMGGDHGAHVTVAAALDFLARDPLTSIILVGNQQAI
                     ESELRRLKAVAESRLRIRHAPEVVAMDESPALALRNKRDSSMRIAVDLVKDGEALACV
                     SAGNTGALMAISRFVLKTLPGIDRPAIASVLPTLKGHTYVLDLGANVDCSAEHLLQFG
                     IMGSELVASVEHRERPTIGLLNIGEEDIKGNEVVKRAAELLRQSGLNFYGNVEGDDIY
                     KGTTDVVVCDGFVGNVALKTSEGLAQMLATYLREEFSRNALTRIAGAIALPVLNAFKR
                     RVDHRRYNGAMLLGLRGIVVKSHGSADAFAFRFAIERAVGEARTGVLAHIADRLSLIQ
                     KSAA"
     gene            3817..>4425
                     /locus_tag="VFY80_09680"
     CDS             3817..>4425
                     /locus_tag="VFY80_09680"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX6008223.1"
                     /translation="MSTYSRIVGTGSYLPAKVLTNDDLERMVDTTDEWIYTRTGIRQR
                     HIAADNENASDLALAASRKAIDAAGIRPQDIGLIIVATTTSDMIFPSTACILQAKLGV
                     RNSPAFDVQAVCSGFVYGLATADHFMRGGQYGYALVVGTEAYSRILDWKDRSTCVLFG
                     DGAGAVVLRQDRTPGIMSSHLHADGSYADILSVPGTVSSGQVK"
BASE COUNT          865 a         1307 c         1448 g          805 t
ORIGIN      
        1 gtctgcgcga gaagtatccg aacattccgc tgtatgccgt cgtggaagac atctgtgcat
       61 ccggcggcta ctatgtcgcc gtggccgccg atcagatata cgtggacaag tcgagcatca
      121 tcggttcgat cggcgtgctg atggacggtt tcggcttcac ggggctcatg gacaagctgg
      181 gtgtcgagcg ccggcttctc gcggcggggg aaaacaaagg tttcctcgat ccgttctcgc
      241 ccatcgagga gtcgcagaag gcacatgcgc agaacatgct caacgagatc cacgaccagt
      301 tcatcaacgt ggtgcgccag gggcgcggca accggctgaa ggaaaaccct gaaatgttct
      361 ccgggcttct gtggatcggc accaagagcg tcgaactcgg actcgcggat gggctgggca
      421 gcgtcgagta cgtggcgcgc gaggtgatca aggccgaaga catagtcgat ttcaccccgc
      481 gggaaaatat tgccgagcgg gtcgcgcgcc gatttggtgc ggcgatggcc gaaacgctgt
      541 tcaagctggc cggactgtcg gcctacgcgc tgcgctgaga ctgcagtaga gcagaaatac
      601 tgcgggccgc cgatcgaggt ggggcggcgt cttgcgccac gcggcgatcg gttgtgtccg
      661 tatgaattca gagggcagcg aaagatcggc cgcgatgcac aacagcgtgt cgtcgcggca
      721 gctttccagg atggcgcgca tgaggctgtc gttgcgatac ggcgcttcga tgaagatctg
      781 cgtgctgtcc ctgcgctggg aggcgtgttc cagttcctta agtctcgtgg cgcgctcctc
      841 gccgcgcacg ggcagatagc cgtggaagtc gaagcgttgg ccgttcaggc ccgaagccat
      901 taatgcgagc aggaccgcgg acggaccgac aaggggaacg acccgtatgc ccgtttggtg
      961 cgcgagacga acaagcaacg cacccgggtc ggcgacagcc ggcgcgcccg cttccgacag
     1021 caggccacag tcgcgcccgg caaggagtgg agccagcagc tgggtcatcg ccgatgcgcg
     1081 cgtgtgctca tccagcacgt gcatctcggt cgtcggcaac gcatgagggt atccggccgc
     1141 tttcagaaac tgccgtgcgc ttttagcgtt ctcgacgatg aaaacggtga gggcaagaag
     1201 ccgctcgcga gtttttggcg gcagtacgtc gccgggatcg ccgccgagcc ccgtgggtac
     1261 aaggtaaagc gtaccgggct gctgcatttt cgcttccccg ttcagagggc gttgatgccc
     1321 tcgttcgcga gcatcgtcac gagctgcatg agcggaaggc cgatcaaggc ggtcgggtcg
     1381 tcgctcgaaa cggtttctat cagagcgata ccgagccctt cgatgcgcgc gctgccggtg
     1441 cagtcataag gacgttcgcg gacgagatag cgttcgatct gctcatcggt gtaaggccgg
     1501 aactgcacgg cagtggtcac atcggcgagc tgcatgcggc ccgaagccgt attggcgagg
     1561 caaattgcgg tgtggaatac aatccgcttg ccgcgcacct gccggagctg gcgcacggca
     1621 ttctcgtgat cacccggttt gcccagcttc acgccctcga cttccgcgac ctgatccgag
     1681 ccgattacca gagcggcgtt tgaagggctt acggcacggg ccttggattc ggcgagtcgc
     1741 agtgccatgc gttcggctgt ctctccgggc aactcccttt cgtccacgct tggaacgatg
     1801 acctcgaagg gcaatcccaa acgttcgagc agcgcacggc ggtacggtga actggaagcg
     1861 agaacgaggc gcgtgtgcat gaggattcag gcggtgcggc gatgccggaa acatcgcgag
     1921 aggacgaacc taagtgcctg ctgactttga ttttttccca gctttctctt tgacagcatg
     1981 gcgcaaaaat actatcatgc gcggcttatg tctgcacaga ctgtcatcga tagtctggaa
     2041 ttcgcgcgtg cggcacagca gttgcaaggc agtgtcccgg ttgcgagttt gacaaggctg
     2101 caggattgcc tgtcggacaa tggcggtgaa gtgagattca gggtgtacgg cgcccgcgat
     2161 gaggagcgcc ggccggtgct cgaggtggag atcagcggaa tgctccatct gcagtgccag
     2221 cgctgtcttg gcccgctcga ctatccgctg catgtccgaa acaccctgtt gctggttcgc
     2281 ccgggcgaga atcccggccc ggacgacgac gatccggaag cgcccgacta tatcgaggcg
     2341 agcgcggagc aggatgttgc ggcgctgatc gaggacgaga ttttgctcag cctgccgttt
     2401 gcgccgcgac atccggagag cacctgccgc ggcgaatggg gagcaggcga gcgaagcggg
     2461 cacaaggaat cggcattcgc caggctggcg gagctcagaa actcaggaca caaagagtaa
     2521 ctttcaggag tattaaatgg ccgtccagca gaacaagaag tcgccgtcga agcgcggcat
     2581 gcaccgctcg cacgactctc tcaccaatcc gccgctggcc gtggagccca cgaccggcga
     2641 agtgcatctg cggcaccaca tcagccccaa cggctactac cgcggaaaga aggtcatccg
     2701 gaacaagggc gaataggccc gcgtgcgccg cgaggcgtga gtccacaccg gcgtccgtgt
     2761 cccccactac aacgataagg gacgcggcaa ggagacttgc acaatggatg tgaccgttgc
     2821 catcgactgc atgggtggcg atcacggcgc gcacgttacg gtcgcggctg cactggactt
     2881 tcttgcgcga gatcctctca cttcgatcat cctggtcggg aaccagcagg cgatcgaatc
     2941 ggaattgagg cgactcaagg ctgtagcaga gtcgcgcctg cgcatacggc acgctcccga
     3001 ggtggtggcg atggacgaat cgcccgcgct tgcgctccgc aacaagcggg actcctcaat
     3061 gcgcatcgcc gtcgatctcg tgaaggatgg cgaagcgctc gcctgcgtga gtgccggaaa
     3121 caccggtgcg ctcatggcga tttcgcgctt cgtactgaaa acgctgccag gcatcgatcg
     3181 gccggccatt gcaagcgtat tgcccacgct caaaggccac acgtacgtgc tggatctggg
     3241 cgcaaacgtg gactgcagcg cggagcacct gctgcaattc ggcattatgg ggtcggagct
     3301 ggtcgcctca gtcgagcacc gcgagagacc gaccatcggg ctgctcaaca tcggcgagga
     3361 agatatcaag ggcaacgagg tcgtgaagcg tgcggcagag ctgctacgcc agagcggcct
     3421 caacttctac ggcaacgtcg aaggcgatga catctacaag gggacgacgg acgtcgtcgt
     3481 ctgcgacggc ttcgtcggta atgtcgcgct gaaaacgtcc gaggggctcg cccagatgct
     3541 cgccacgtac ctgcgcgagg agttttcgcg caatgcgttg acccggattg cgggtgcgat
     3601 cgcgctgccg gtgctgaacg cgttcaagcg gcgcgttgat catcgccgct acaacggggc
     3661 gatgctgctg gggttgcgcg gcatcgtggt gaaaagccac gggtcggcgg acgcctttgc
     3721 attccggttt gccatagagc gtgcggtggg cgaggcgcga accggcgtgc tcgcgcacat
     3781 cgccgaccgc ctatccctga tccagaagag cgctgcgtga gcacttattc ccgcattgtc
     3841 ggcaccggca gctacctgcc cgcaaaagtg ctgaccaacg atgatctcga gcgcatggtc
     3901 gacaccaccg acgagtggat ctacacccgc acgggcatcc ggcagcgcca cattgctgcc
     3961 gacaacgaaa atgcaagcga tcttgcgctc gccgcgagcc gcaaggccat cgatgcggcc
     4021 gggattcgtc cgcaggacat cgggctcatc atcgtcgcaa cgacgacatc cgatatgatc
     4081 ttccccagta ccgcgtgcat tttgcaggca aagcttggcg ttcggaacag cccggcgttc
     4141 gatgtgcagg cggtgtgcag cggcttcgtg tatgggctcg cgaccgccga tcacttcatg
     4201 cgcggcggtc agtatggata tgccctcgtc gttggaacgg aagcgtactc ccgcattctc
     4261 gactggaaag atcgatcgac ctgcgtactc ttcggcgatg gtgccggggc ggtcgtcctc
     4321 aggcaggacc ggacaccggg aatcatgtcc tcgcatctgc acgccgacgg cagctatgcc
     4381 gacatccttt ctgtcccggg cacggtttcc agcgggcagg tgaaa
//
LOCUS       DASURG010000538         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1522349, whole genome shotgun sequence.
ACCESSION   DASURG010000538 DASURG010000000
VERSION     DASURG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522349"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFY80_09685"
     CDS             <1..889
                     /locus_tag="VFY80_09685"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX6008224.1"
                     /translation="GVHLGHQAMLGELTRAARARELAACVLTFEPHPREFFAPDQAPV
                     RLTSLREKIELLAARGIDRLHVCRFNYELAQVSAPQFIERVLVAGLGVRWLLVGDDFR
                     FGARRAGNFVTLARAAPRFGFEVQAMDSVALDGERVSSTAVRNALAAGDLARAARLLG
                     RPYSISGRVVRGDGLGRKLGFPTANVQMKHNRPPLSGIFAVEVFGAEDRVLRGAASLG
                     VRPTVTERGRPVLEVYLLDYEGELYGRHLRVDFLHKFRDEEKYADLAALTRQIALDVE
                     NARAYFAGRDAGRARRVTR"
     gene            976..>2372
                     /locus_tag="VFY80_09690"
     CDS             976..>2372
                     /locus_tag="VFY80_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6008225.1"
                     /translation="MADYKKTLNLPDTPFPMRGDLAKREPRMLKRWQERKFYERIREK
                     SKGRPRFVLHDGPPYANGDIHIGHAVNKILKDIIVKSKSMAGFDAPYVPGWDCHGMPI
                     EVQIEKTHGKNLPTEETQRLCRAYAAEQIERQKKDFQRLGVLGDWDHPYLTMAYKNEA
                     DEIRTLGALLAKGYVYRGLKPVNWCFDCGSALAEAEVEYEDRTDPAIDVAFPFAEPDK
                     IARAFGLERLPDKPGFAVIWTTTPWTLPGNQALNVHPEYDYALVDTDRGMLIVANDIL
                     MHNEAEWAQSGDDLKIPPLLRKYHVKTWKVLALCKGKALELIPFKHPFYDREAPVYLG
                     DYVTLDTGTGIVHSAPAYGVEDFDSCRRYGMKDDEILTPVMGDGRFVSSLPLFGGMSI
                     WAANRKIVEHLAEKGVLFAKSDFVHSYMHCWRHKTPVILRATTQWFAGMDEVPGFRGA
                     KPEESLRATALRGVER"
BASE COUNT          441 a          756 c          773 g          402 t
ORIGIN      
        1 cggcgtccat cttgggcatc aggcgatgct cggggagctc acgcgcgccg cgcgcgcgcg
       61 cgaacttgcc gcctgcgtgc tgacattcga gccgcatccg cgcgagtttt ttgcgccgga
      121 ccaggcaccc gttcgcctca cctcgctgcg cgaaaagatc gagctactcg cggcacgcgg
      181 catcgaccga ctgcacgtct gccgcttcaa ttacgagctg gcgcaggtga gcgcgccgca
      241 gttcatcgag cgcgtgctgg tggcgggact cggcgtgcgt tggctgctgg tgggcgacga
      301 ctttcgcttc ggtgcgcgcc gggcggggaa tttcgtgacg cttgctcgcg ccgcgcccag
      361 gttcggtttc gaagtgcagg cgatggacag cgtggcgctc gacggcgagc gcgtctcgag
      421 caccgccgtt cgcaatgccc ttgcggcagg cgatctcgcg cgcgcggcac gcctcctcgg
      481 acggccgtac agcatcagcg gccgcgttgt gcggggggat ggcctcgggc gcaagctcgg
      541 cttcccgacg gccaacgtgc agatgaagca caaccggcct ccgctcagcg gcatttttgc
      601 cgtggaagta ttcggcgcgg aagaccgcgt actgcgcggc gcagcaagcc tgggcgtgcg
      661 gcccaccgtc accgagcgcg ggcggcccgt gctcgaagtg tatctcctcg actacgaggg
      721 tgagctctac gggcggcacc tgcgcgtcga tttcctccac aagttccgcg acgaagagaa
      781 gtatgcggat ctcgccgcac tcacccggca gattgcgctc gacgtggaga acgcgcgcgc
      841 gtacttcgcg gggcgcgacg ccggaagggc gaggagagtc acccgctgaa gcagcacgcc
      901 cgctgcttgc ggtaacatcg cagctttttg cgccgccgtt tcctgcgcga cacccagaga
      961 cagacgcact cccgaatggc tgactacaag aaaacgctga acctgcccga cacgccgttt
     1021 cccatgcgcg gtgacctcgc aaagcgagag ccccggatgc tgaaacgctg gcaggagcgc
     1081 aagttctatg agcgcatccg ggaaaagagc aaaggccgac cgcgcttcgt gctgcacgac
     1141 ggtccgccgt acgcaaacgg tgacattcac atcgggcacg cggtcaacaa gatcctgaag
     1201 gacatcatcg tcaagagcaa gtcgatggcg ggattcgatg cgccctacgt cccgggatgg
     1261 gactgccacg gcatgccgat cgaggtgcag atcgagaaga cgcacggcaa gaacctgccg
     1321 accgaagaaa cccagcgcct gtgccgtgcc tatgctgccg agcagatcga acgccagaag
     1381 aaggatttcc agcggctcgg cgtgctgggt gactgggatc acccctacct cacgatggcg
     1441 tacaagaacg aggccgatga gatccgcacg ctcggtgcgc tgctcgccaa agggtacgtc
     1501 taccgcgggt tgaagcccgt caactggtgc ttcgactgcg gctccgcgct tgcggaagcg
     1561 gaagtcgagt acgaagaccg caccgatccc gcgatcgatg tggcttttcc gttcgccgaa
     1621 ccggacaaga tcgcacgcgc tttcggactc gagcgccttc cggacaagcc cggcttcgcg
     1681 gtgatctgga cgacgacgcc gtggacgctc cccggaaatc aggcgctcaa cgtccatccc
     1741 gaatacgact atgcgctcgt cgacaccgat cgcggcatgc tgatcgtcgc gaacgacatc
     1801 ctgatgcaca acgaagcgga atgggcgcag tccggtgacg atctcaagat tccgccgctg
     1861 ctccgcaagt atcacgtcaa gacttggaag gtcctcgccc tgtgcaaagg gaaggcgctg
     1921 gaactcatcc ctttcaagca tcccttttac gaccgcgagg ctcccgtcta tctcggtgac
     1981 tacgtgacgc tcgataccgg aacgggcatc gtccattcgg caccggctta cggcgtcgag
     2041 gatttcgact cgtgccgccg atacggcatg aaggacgatg agatcctcac gccggtcatg
     2101 ggcgacggcc gattcgtgtc gagtctcccg ctattcggcg gcatgtcgat ctgggccgca
     2161 aaccggaaga tcgtcgagca cttggcggaa aagggcgtgc ttttcgcgaa gtccgacttc
     2221 gttcacagct acatgcactg ctggcggcac aagacgccgg tcatcctgcg cgcgacgaca
     2281 cagtggtttg cggggatgga tgaagtgccc ggtttccgcg gcgcgaagcc ggaagagagt
     2341 ttgcgcgcga cggcgttgcg gggcgtggag cg
//
LOCUS       DASURG010000539         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1010650, whole genome shotgun sequence.
ACCESSION   DASURG010000539 DASURG010000000
VERSION     DASURG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010650"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFY80_09695"
     CDS             complement(<1..695)
                     /locus_tag="VFY80_09695"
                     /inference="COORDINATES: protein motif:HMM:NF016532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HEX6008226.1"
                     /translation="MMGTRFFTELTVTWLDVLAFLFFVAAWVGYATFSTWRGRQVPSL
                     HSSMDGYRRLWMVRMIERDNRMVDVNIMRNLTRSSQFFASTTMLILGALVALTGYVQQ
                     ALDVVSGLPFTVKGTQRVIEIKILLLVVIFVYAFFKFSWGIRQLGFCSILVGAAPKPP
                     QGDPEQHAPHINRIARITSSAGVNFNNGLRAYYFALAALAWFLHPWLMVVATAWVVYV
                     LHRREFRSKTLRAL"
     gene            complement(725..1831)
                     /locus_tag="VFY80_09700"
     CDS             complement(725..1831)
                     /locus_tag="VFY80_09700"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEX6008227.1"
                     /translation="MPTFRYGHASAPDWRDAAKSCLEQLGGGSATLGFLYITDLLADH
                     AADIHAWFKVQTGVAHWTGAVGIGVCATGQEYLDEPAIAVMVADFDPGTFRVFSGISR
                     AEQVDRAAIKCGNVPANFAVIHGDPGNREVPDLVTKLAERVESGFLVGGLVSSRRQNI
                     QIADGVVENGISGVSFSDAVTVATRLTQGCSPIGPKHGITACQRNVIVSLDGEPALDV
                     FKEDIGETLARDLSRVGGHVFAGLPVPGSDTGDYLVRNLVGIDTTHKLVAIGDLIEPG
                     GSIMFCRRDNSTAREDMTRMLESIRQGLFTAPRGGVYFSCLGRGASLFGPDSRELKMI
                     RDGLGDFPLVGFFCNGEISHNRLYGYTGVLTLFV"
     gene            1963..2769
                     /locus_tag="VFY80_09705"
     CDS             1963..2769
                     /locus_tag="VFY80_09705"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX6008228.1"
                     /translation="MARALESAVSAVPFLQRRDESGVSTLTLSRPQQYNALSLNLIRE
                     LEAALAAIAHDESVRVVILAGAGRAFCAGHDLKELRTHGDPQFAREVFERCARLMVGL
                     TRLPQPIIARVHGFAFAAGCQLVAQCDLAVASTDAQFATSGIKFGLFCNTPGVGLARN
                     VSRKQAMEMLLTGEPIDAQTALARGLVNRVVAPDALDAEIGKLTRAVLDKTPVAVAAG
                     KRAFYQQIELGLEEAYQVATEAMICNLATEDAAEGIDAFSQKRPPQWKNR"
BASE COUNT          576 a          917 c          907 g          508 t
ORIGIN      
        1 agcgccctca gcgttttcga gcggaactcg cggcgatgga ggacgtagac cacccaggcc
       61 gtcgcgacga ccatgagcca tggatgcagg aaccacgcga gcgcggcgag cgcaaagtag
      121 taagcgcgca ggccattgtt gaaattgacc cctgccgacg aggtgatgcg cgcaatgcgg
      181 ttgatgtgcg gcgcgtgctg ctccggatcg ccctgcggcg gcttgggcgc ggcgccgacg
      241 agtatcgaac agaagccgag ctggcggatg ccccacgaaa acttgaagaa tgcgtagacg
      301 aagatcacga ccagcagcag gatcttgatc tcgatgacgc gctgcgtccc ctttaccgtg
      361 aagggcagcc ctgacaccac atcgagcgcc tgctgcacgt aacccgtcaa ggcgacgagc
      421 gcccccagta tcagcatggt ggtggaggca aaaaactgcg agctgcgagt gagattgcgc
      481 atgatattga catccaccat gcggttgtcg cgctcgatca tgcgcaccat ccagagccgg
      541 cgatagccgt ccatgctcga gtgcaggctt ggcacctgcc tgccgcgcca agtcgagaaa
      601 gtggcatacc cgacccacgc ggcgacgaag aacaggaacg ccagaacatc gagccaggtg
      661 accgtcagtt ccgtgaagaa ccgcgtgccc atcatgcgcg cctcccgcgc gtcgcgcttt
      721 tgttctagac gaagagcgtc aacacgcccg tatagccgta gagccggttg tgcgagatct
      781 ctccgttgca gaagaatccg accagcggaa aatcgccgag gccgtcgcgg atcatcttga
      841 gctcgcgcga atcgggaccg aagaggctcg ctccgcgccc gaggcaagaa aaatatacgc
      901 cgccacgcgg cgcggtaaac aggccctgcc ggatgctctc cagcatgcgg gtcatgtcct
      961 cgcgcgcggt cgaattgtcg cggcggcaga acatgatgct gccgccgggc tcgatgagat
     1021 cgccgatcgc gacgagcttg tgcgtcgtgt cgatgcccac cagattgcgc acgagataat
     1081 cgcccgtatc gcttccgggc acggggagtc cggcaaagac atggccgccc acgcggctca
     1141 agtcgcgcgc gagcgtctcc ccgatgtcct ccttgaagac gtcgagcgcc ggctcgccat
     1201 cgaggcttac gatcacgttg cgctggcacg ccgtgatgcc atgcttggga ccgatcggcg
     1261 agcatccttg cgtaaggcgc gttgccacgg ttacggcatc cgaaaagctc acccccgata
     1321 tgccgttttc cacgacgccg tcggcgatct ggatattctg ccgccgggag ctcacgagcc
     1381 cacccacgag aaaaccgctt tcgacgcgtt cggcgagctt ggtgaccaga tcgggcactt
     1441 cgcggttacc cggatcgccg tggatcaccg caaaattggc cggcacattg ccgcacttga
     1501 tcgcagcgcg gtctacctgc tcggcgcgcg agattcccga aaagacgcga aacgttccag
     1561 gatcgaagtc cgccaccatc accgcgatcg cgggttcgtc gaggtattcc tgccccgtcg
     1621 cgcacacgcc tatgccgact gcacctgtcc aatgcgcaac gcccgtctga accttgaacc
     1681 aggcgtggat gtccgcggca tggtcggcaa ggagatccgt gatgtaaaga aatccaagcg
     1741 tcgcgctgcc tccaccgagc tgctcgaggc acgacttcgc cgcatcccgc cagtcgggcg
     1801 cgcttgcgtg gccgtaacga aacgtgggca tggccgattc cgatggggat ggaggcaaat
     1861 cctagcatgt gtggcacgaa actcgccaac cgaacgtcgt gggggatccg cctggcggca
     1921 agtcgctata attcctctcc atctcttttc atggaggcag ccatggctcg cgcgctcgag
     1981 agcgccgttt ccgccgtccc cttccttcaa cgaagagacg agagcggtgt ctccacgctg
     2041 acgctcagtc ggccgcagca gtacaacgcc ctctcgctca acctcattcg cgaactggaa
     2101 gcggcattgg ccgcgatagc gcacgacgaa tcggtgcgtg tggtgattct cgcgggcgca
     2161 ggaagggcat tttgcgccgg ccacgatctc aaggaactgc gcacacacgg tgatcctcag
     2221 tttgcgcggg aggtgttcga gcggtgtgcg cggctgatgg tcggcctcac gcgcctgccc
     2281 cagccgatta tcgcgcgcgt gcacggattc gcgtttgctg ccggctgcca gctggtcgcg
     2341 cagtgtgatc tcgccgtcgc atcgacagat gctcagttcg cgacctcggg tatcaagttc
     2401 ggtctcttct gcaacacccc cggcgtcggt ctcgcgcgca atgtgtcgcg caagcaggcg
     2461 atggaaatgc tgcttacggg ggagccgatc gatgcccaga ccgctcttgc gcgcgggctg
     2521 gtgaatcggg tggtcgcgcc ggacgcgctc gatgccgaaa tcgggaagct cacgcgcgca
     2581 gtactcgaca agacgccagt cgccgtggcg gccggaaagc gcgctttcta ccagcagatc
     2641 gaattgggac tcgaagaggc gtatcaggtc gcgaccgagg cgatgatctg caatctcgcg
     2701 accgaggatg cagccgaggg aatcgatgcg ttctcgcaaa aacggccgcc tcaatggaag
     2761 aacaggtaaa cggagcccgt cagatgaatt caggcgcacg catttgcact tgtcattcct
     2821 gcggaagccg gaatccagaa aacttcgagc tcaattcggc ctggattccg gatcgcgctt
     2881 gtcgctgccg cgcgtccgga atgacgat
//
LOCUS       DASURG010000540         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1522651, whole genome shotgun sequence.
ACCESSION   DASURG010000540 DASURG010000000
VERSION     DASURG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522651"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFY80_09710"
     CDS             <1..397
                     /locus_tag="VFY80_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008229.1"
                     /translation="TLPFGVTWTYDRGYMVAGSDRASAERAIATRNGGSPLVWSQEFL
                     AQLPSSAGIHPSAFAWLNTKGAFELLGSFTQSQALKDLLAARDPALVVFDGTADRIHA
                     ASRARISGLIMDAMLLESLSGSSQQSGTQ"
     gene            402..1379
                     /locus_tag="VFY80_09715"
     CDS             402..1379
                     /locus_tag="VFY80_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6008230.1"
                     /translation="MSVPVVELENLKVRLGRREILHGITCRLGVSGTGKAVGLLGPNG
                     AGKSTLIQTLLGFLRPSDGRARILGLDCQHSAVEARSRIGYMPENDSFIGDMTAVAFL
                     RQMAELSGLPPEAALEKAHQVLFHVGLGEARYRELRTYSLGMKQMAKLAQAIVHGPQL
                     VVLDEPTNGLDPAARRRMLKLITEMKSEQGMNVLVCSHLLRDIEEVCDEAVIMKDGAI
                     VHHCNLEEERRSNRSFVELEVTGDDGNLRAALPEIGAEGVSEGHGRWRIVLPPGAGIE
                     AIWGLLGRQNLLVRKLSHRRDTLEEIFLKAMGHLAHTPGERAVADVSGT"
     gene            1460..2254
                     /locus_tag="VFY80_09720"
     CDS             1460..2254
                     /locus_tag="VFY80_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008231.1"
                     /translation="MVLPRYAWRRLYQQRLVLLLTVLSFLWPLLCAGFVYLSNHTELL
                     YGLDKEFIQFIQVDGRFFSIFMYVQSGFAVFLAALAGPGLIAPDLANNALPLYFSRPL
                     TRWSYALARLTVLVGMLSVVTWIPGLILFGLQVGLTGGGWFWANWSLGGGMVAGFLLW
                     LLVLSLVAMASSAYVKWRIIAAAVSLAFFFILSGVGEMIDTVLRVRWGHIIDPAWTVN
                     QVWSKLLGVPLLEGPGAAASALALTTLIVLLAAVIARKLRPVEIVS"
     gene            2251..>3136
                     /locus_tag="VFY80_09725"
     CDS             2251..>3136
                     /locus_tag="VFY80_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6008232.1"
                     /translation="MSAPAVVFEEVSKFYGEVLGVNRVTLNIPPGLTSLVGPNGSGKT
                     TIMNLMTGLIHPDNGRILMRGISPRDPETLMRITGYATQYDTAPRWATGFTFITTGLL
                     LFGYKRSEAEEKAWKALERVGLTEAANRKVAGYSKGMRQRVRLAQAIAHDPEVLVLDE
                     PLNGLDPLVRAETIALFRSWAAQGKHVIISSHVLQEVDLISDQVVLMANGTIVAEGTI
                     RTVREEIEEHPSQYLVRCRDASDVAARLFNEDHVTEVRLNRDRMGMLVMTSDREEFAR
                     ALGRIAQDGHVIEGVVPAD"
BASE COUNT          543 a          959 c         1051 g          583 t
ORIGIN      
        1 gactcttccg ttcggcgtca cctggacgta cgatcgcgga tacatggtcg cgggctcgga
       61 tcgcgcgtct gccgagcggg ccattgcaac caggaatggc ggttcgccgc tggtctggtc
      121 gcaggaattc ctggcgcagc tcccgtcgtc agccgggatt cacccgtcag ccttcgcgtg
      181 gctgaacacg aagggggcgt tcgaactcct ggggagcttc acgcagagcc aggcgctgaa
      241 ggatctgctc gccgcgcgcg atcccgcact ggtggtgttc gatggaacgg cggaccggat
      301 ccacgccgcc agccgcgctc gtatatcagg attgatcatg gacgccatgc tgctcgagag
      361 cctgagcggc tcgagccaac agtccggaac gcagtgagaa cttgagcgta ccagttgtag
      421 aactcgagaa cctgaaggtc aggttggggc ggcgggagat ccttcacggg atcacctgcc
      481 gtctcggcgt ttcaggcacc ggcaaagccg tgggacttct gggtccgaac ggcgcgggca
      541 agtccacgct cattcagacc ctgctcggct ttctgcgccc gagcgacggg cgcgcccgca
      601 tccttggact cgattgccag cacagcgcgg tcgaggcgcg atcgcgcatc ggctacatgc
      661 ccgagaacga ttcgttcatc ggcgacatga cggccgtagc cttcctccgc cagatggcgg
      721 agctctccgg actccctcct gaagcggcgt tggagaaagc ccaccaggtc ctctttcatg
      781 tgggactcgg cgaagcccgc taccgtgagc tccggacgta ctcgctcggc atgaagcaga
      841 tggcgaagct cgcccaggcg atcgtccacg gtccgcagct cgtcgtgctc gatgaaccga
      901 ccaacggcct ggatcccgcc gcgcgccggc gcatgctcaa gctgattacc gagatgaaga
      961 gcgagcaggg gatgaacgtg ctggtctgct cgcacctgct gcgcgacatc gaggaggtgt
     1021 gcgacgaggc cgtgatcatg aaggacggcg cgatcgtgca tcactgcaat ctcgaggaag
     1081 agcggcggtc gaaccgttcc ttcgtcgaac tcgaggtgac gggcgacgat ggaaacctgc
     1141 gagccgcgct gcccgagatc ggcgccgaag gtgtctccga agggcacgga cgctggcgca
     1201 tcgtcctgcc gccaggtgcc gggatcgagg caatctgggg actcctcgga cggcagaatc
     1261 tcctcgtgcg aaagctctct cacaggagag acacattgga ggaaatcttc ctcaaggcaa
     1321 tgggacatct ggcgcacacg ccgggagagc gtgcggttgc agacgtcagt ggaacctgac
     1381 ggcgaatcgg aaaatggctg tctacaaacg gggatatcaa cgatacgacg gaccgctcac
     1441 cgggccgttg accagattca tggtgctgcc tcgctacgcc tggcgacggc tctatcagca
     1501 gcgtctcgtg ctcctgctca cggtgctgtc gttcctgtgg ccgcttctct gtgccggatt
     1561 cgtctatctg tcgaaccata cggagctgct gtacggcctc gataaagagt tcattcagtt
     1621 catccaggtc gacgggcggt tcttctcgat cttcatgtac gtgcagtcag ggtttgccgt
     1681 gttcctggcg gcgctggccg ggccgggcct gattgcgccg gacctcgcca acaacgcgtt
     1741 gccgctctat ttcagtcggc cgctcacgcg ctggagctac gccctcgcgc ggctgacggt
     1801 gctcgtcgga atgctctcgg tcgtcacctg gatcccgggg ctgatcctgt tcgggttgca
     1861 ggtggggctg accggtggcg ggtggttctg ggcgaactgg tcgcttggcg gcggcatggt
     1921 cgccggcttt ctcctctggc tgctcgtgct gagcctcgtg gcgatggcga gttctgcgta
     1981 cgtgaagtgg cggatcattg ccgccgctgt cagcttggcc ttcttcttca ttctgagcgg
     2041 tgtgggcgag atgatcgata ccgttctcag ggtgaggtgg gggcacatca tcgatccggc
     2101 ctggacggtg aaccaggtgt ggtcgaagct gctcggcgtg ccgctgctcg aaggtccagg
     2161 tgccgccgcg tccgcgcttg cgctgaccac gctgatcgtg ctcctcgctg cggtgatcgc
     2221 gcgcaagctg cggccggtgg agatcgtgtc atgagtgcac cagctgtggt cttcgaggaa
     2281 gtgtcgaagt tctatggtga ggtgctcggc gtcaatcgcg tcacgctgaa catcccgccg
     2341 gggctcacga gcctggtcgg cccgaacggt tcgggcaaga ccacgatcat gaacctcatg
     2401 accggtctca tccatcccga taacggccgg atcctgatgc gcgggatctc gccccgcgat
     2461 cccgaaacgc tgatgcgcat caccgggtac gccacgcagt acgacaccgc gccgcgatgg
     2521 gcaaccgggt tcaccttcat cacgacaggg ctgctcctct tcggatacaa acgatcggaa
     2581 gccgaggaaa aagcctggaa ggcgctcgag cgcgtgggac tgacggaggc ggcgaatcgc
     2641 aaggtggcgg gatattccaa gggcatgcga cagcgcgtgc gactggcgca ggcgatcgcg
     2701 cacgatcccg aggtgctcgt gctcgatgag ccgctcaacg gcctcgaccc gctcgtgcgc
     2761 gccgagacga tcgcgctatt tcgctcgtgg gcggcccagg ggaagcacgt catcatctcc
     2821 agccacgttt tgcaggaagt ggacttgatc agcgatcagg tcgtgctgat ggccaacggg
     2881 acgatcgtcg ccgaagggac gatccgcacc gtgcgcgagg agatcgagga gcatccgagc
     2941 cagtacctgg ttcgatgccg cgacgcctcc gatgtggcgg cccggctgtt caatgaagac
     3001 cacgtgaccg aggtcaggct gaaccgcgac cggatgggca tgctggtgat gaccagcgac
     3061 cgtgaagagt tcgcgcgcgc cctcgggcgc atcgcgcagg acggccatgt catcgaaggg
     3121 gtcgtgccgg ccgacg
//
LOCUS       DASURG010000541         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1522764, whole genome shotgun sequence.
ACCESSION   DASURG010000541 DASURG010000000
VERSION     DASURG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522764"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFY80_09730"
     CDS             complement(<1..494)
                     /locus_tag="VFY80_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008233.1"
                     /translation="MKRTQLRSLQGPALVLLVVVLAACAGVYYTNGLLQHARQQLAQQ
                     ERLLKDARNQLYQSGEEREVIGRYLDRYTELQRIGFVGEEQRINWLDGLRIANQRAEL
                     FGVDYQISEQRAYSHARELNPGQLTMHQSVMKVRFGLLHEGDLMQFFDTLAQSGAGVF
                     HIDQ"
     gene            complement(491..2056)
                     /locus_tag="VFY80_09735"
     CDS             complement(491..2056)
                     /locus_tag="VFY80_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008234.1"
                     /translation="MAEKLLVCVAAEQVTAGRWRRGHLGECRVFTNDQLGFAAFEKYI
                     SQFSGVPAYLMVDAVEEDYRFETLPHAFGSDRSEMVRRKLRQHYRNLPYVNASFLGRD
                     AERRRDDRYLFSALTNPDLIVAWLQALLVRELPLAGVFMLPMVSAALVTSLRLKAPNV
                     LLVAQHASGLRLTFLRDRQFRLSRLTRGDAGRTGSPERVIAEEISNTRIYLHALRAAR
                     LDEPLAIVLLDHGDQLGGVAEIIARDNPSLDCMHLKRRALAGRLRVAEPLLAATSDAM
                     YLALLGKHAPSANHAPSDVTGGFGRYRQRRALYAAAGTAALFALGWSGVNLWQTALTH
                     ADREAAVRQTEQLRAAYQEITRQFPATPTAAANLKRAVDIAQQLRAGVRTPDRLMHAV
                     SRALEATPGIAIRRLTWKHGTGDIDAGASVPSGTQGVPGPSGTGGPFKRESALIEAEI
                     RPFRGDYRLAIEQIHSFANHLASHPEVAETRIVKLPLNIDPKLPLSGNTIDKGDSGEG
                     ADFRLAVVLKPPS"
     gene            complement(2102..>2591)
                     /locus_tag="VFY80_09740"
     CDS             complement(2102..>2591)
                     /locus_tag="VFY80_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6008235.1"
                     /translation="NTAAAYFWDYAKLRAPLTGPILQKIILARFASFFALMYESGITV
                     LDALKTCEAIVDNRVIADGVMRAGQQISSGENLSEAFRNLGIFPPLVVRMLHVGESTG
                     ALDTALNNINYFYSRDVRESVDKMLKFMEPALTLGLGVILALIMWSVLSPVYDILGKL
                     KF"
BASE COUNT          487 a          800 c          831 g          473 t
ORIGIN      
        1 cactggtcga tgtggaacac tccggccccg gactgagcca gggtgtcgaa gaactgcatg
       61 agatccccct cgtgcagcag gccgaatctc accttcatca ccgactgatg catcgtcaac
      121 tgtccggggt tgagctcgcg cgcgtgggaa taggcgcgtt gctcgctgat ctggtaatcg
      181 acaccgaaca actcggcgcg ctggttggcg atccgcaggc cgtcgagcca gttgatacgc
      241 tgctcctcgc cgacgaaacc gatgcgctgg agctcggtgt agcgatcgag gtaacggccg
      301 atgacttcac gttcctcgcc ggactggtag agctggttgc gcgcatcctt gagcagccgc
      361 tcctgttgag cgagctgctg ccgcgcgtgc tgaagcagac cgttcgtgta gtagacgccc
      421 gcgcaggcag ccagcacgac gacgagcaga acgagcgccg ggccttgcag tgacctcaat
      481 tgggttctct tcatgagggc ggtttcaaca ccaccgcaag ccggaaatcg gcgccttcac
      541 cggagtcgcc cttgtctatg gtattgccgc tcaatggcag cttgggatcg atgttgagcg
      601 gcaatttcac gatacgcgtt tcagccactt cggggtgcga cgcaaggtgg ttggcgaagg
      661 aatggatctg ctcgatcgcc agacgataat caccgcgaaa gggccggatt tccgcctcta
      721 tgagcgcgct ttcgcgcttg aacggcccgc ctgttccgga ggggccgggg acaccctgcg
      781 tgccggacgg cactgatgcc cccgcatcga tgtcgcccgt tccgtgcttc caggtaagcc
      841 ggcgaatagc gatgccaggc gtcgcctcga gcgcccggct caccgcgtgc atgagccggt
      901 ccggagttcg cacgccggcg cgcagttgct gtgcaatgtc cacggccctc ttgaggttgg
      961 cggccgcagt cggggtggcc gggaactggc gcgtgatctc ctggtaagcc gcgcgcagtt
     1021 gctcagtctg tcgcacggct gcttcgcgat ccgcatgcgt gagcgccgtt tgccacagat
     1081 ttacgccact ccagcccagg gcgaagagcg cggcggttcc ggcggcagcg taaagggcgc
     1141 gccgttgccg atagcgtccg aatcctccgg ttacgtccga tggcgcgtga ttggccgaag
     1201 gcgcgtgctt gccgaggagc gccagataca tcgcgtccga ggtcgccgcg agcagcggtt
     1261 ccgcgacacg cagtcggcct gcaagcgccc ggcgcttgag atgcatgcag tcgaggctcg
     1321 ggttatcccg cgcgatgatc tcagcgacgc cacccagctg gtcgccgtga tcgagcagga
     1381 cgatggcaag cggctcatcc agacgcgcgg cccgcaatgc gtggagataa atccgggtgt
     1441 tggagatctc ctccgcgatg acgcgttcag gactgcccgt gcggccggcg tcgccgcgcg
     1501 tgaggcgact caggcgaaac tgtcgatcgc gcagaaaagt gaggcggagt ccgctcgcgt
     1561 gctgcgcgac gagaagcaca ttcggggctt ttagccgcag cgacgtaacg agcgccgcac
     1621 tgaccatggg cagcatgaaa acgccggcga gaggcaattc tctcaccaag agcgcctgca
     1681 accatgccac gatcaggtcc gggttggtga gcgcggaaaa gagatagcga tcgtcccgac
     1741 gccgttcggc gtcgcgtccg agaaatgacg cgttgacgta cggcaggtta cggtaatgct
     1801 gccgcagctt ccgacgcacc atttcgctgc ggtccgagcc gaacgcgtgc ggcagcgttt
     1861 cgaagcgata gtcctcttct accgcgtcga ccatgaggta agccggaacg ccggagaact
     1921 gcgaaatgta cttttcgaac gctgcgaagc cgagctgatc gttggtgaag accctgcact
     1981 cccccaggtg cccgcggcgc caacgcccgg ccgtgacctg ttcggccgct acgcagacga
     2041 gaagcttttc agccatccct gcacaccgtc gccccttcaa gggtcattgt gaagccgggc
     2101 atcagaattt caacttgccc aaaatatcat acaccggcga cagtaccgac cacatgatga
     2161 gcgcaaggat gaccccgagc cccagggtga gcgcgggttc catgaacttg agcatcttgt
     2221 ctacggactc gcgcacgtcg cgactgtaga agtaattgat gttgttgagc gcagtatcga
     2281 gcgcgcccgt gctctccccc acgtgcagca tgcgcacaac aaggggcggg aagattccca
     2341 gattgcggaa cgcctcggac aggttttcac ccgaggagat ctgctggccg gcgcgcatga
     2401 ctccgtcggc gatgacgcgg ttgtccacga tcgcctcgca tgtcttgagt gcatcgagga
     2461 ccgttattcc ggactcgtac atgagcgcaa aaaaactcgc gaatcgcgcg aggatgattt
     2521 tctgcaggat cggcccggta agcggcgcac ggagcttcgc atagtcccag aagtaggccg
     2581 ctgccgtgtt t
//
LOCUS       DASURG010000542         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_91357, whole genome shotgun sequence.
ACCESSION   DASURG010000542 DASURG010000000
VERSION     DASURG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91357"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VFY80_09745"
     CDS             complement(<1..1558)
                     /locus_tag="VFY80_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6008236.1"
                     /translation="MTQSARLHPLEAPLEGVSLIEANAGTGKTWTITALYLRLILETE
                     RSVDSILVVTFTEIATAELRDRIRTRLAEARAAFERGARALESGSCRHDDPLLDGLLQ
                     RAADHDRAALQLERALADFDQAPVYTIHAFCQRVLADRAFESAMPFETRILPDESLLL
                     QEIVEDFWRRTLYGASPLFVRCVLESNLTPLKLREDLNGRLWPYLEVRRPVLSGDLDS
                     VESEYEQAYARARAIWRESHDAIAAELAGNRNLHGGRYRADRVREWLEEMDRCLSPER
                     PGITLFERFEKFTPAALAEATKRGANPPGHPFYAACEALRRAHAALEVAHQKRLAILK
                     AELLEYCNTELAARKERLRLQSYDDLLLNLAGALGAGHGSALAQAIRERYTAALIDEF
                     QDTDPVQYRIFRTVYGGSGLPVFLVGDPKQSIYSFRGADIFAYLDARRDAEHRHALDV
                     NWRSERPLLTAINALFARGAAPFVIPDIPFVPSEAAPGSRGSLVLEGESAPPFQFWLM
                     AGENGKARNKD"
     gene            complement(1555..>3510)
                     /locus_tag="VFY80_09750"
     CDS             complement(1555..>3510)
                     /locus_tag="VFY80_09750"
                     /inference="COORDINATES: protein motif:HMM:NF037683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008237.1"
                     /translation="ALLDLPGSRYEAGKVLEILEEPAVRRRFAIGAEDLDTVHDWVRE
                     SGIRWGIDAAHRATFSVPATAEHTWRFGLERLIVGYALPGRGERLFGDILPHDEVEGG
                     LGEILGRFASYAEAAIALNKLPAQCTVSQWAHTLRTILAQFFDPDEQREAEIEALRSA
                     IGALESNAAAGGFQAAIPLEVMKSALRSRLEVPGRAFLSGGVAFCAMVPMRSLPFEVV
                     CLLGMNDTAFPRMRRPHGFDLMTRDFRKGDRSRRDDDRYLFLESLISARRCFYVSYTG
                     QHIRDNSVIPPSVLVSELGDYLARNYAGPGGGDIREHVTTHHPLQPFSPRYFYGDPRL
                     FSYSHALCAAASQLKRGEAGGKPFMSQPLPEPEEGRRTVEMESLIRFLRNPARYFLRE
                     RLGIRLEEADEEMEAREPLTLHGLEDYALKQRLLGVRMHGKPVGSALSLARASGLLPH
                     AQVGALVFERTARAVDEFAGRLEQAWPKARLDPIAFDLESGGMMLRGALSGASPEGLV
                     GYRFAECKPKDLLDAWVRHLVLNALRPVGVATVTRWHLQDKTLQFRPISEARALIAAL
                     LELHWTGLKRPLHFFPASAYAYMRKERNLEAARRAWLSDRARGEDADPYFRLAFRGCD
                     PLDAEFEALAAAVFEPLLAVMTEEVAA"
BASE COUNT          610 a         1137 c         1163 g          600 t
ORIGIN      
        1 cgtccttgtt gcgcgctttt ccgttctcgc ccgccatcaa ccagaactgg aaaggcggcg
       61 cagattcccc ttcgagcacg agagatccac gactgccggg tgcggcctcg gacggaacaa
      121 acggaatgtc gggaatgacg aagggagccg caccgcgcgc aaagagcgcg ttgatcgcgg
      181 tgagcagcgg ccgttcggag cgccagttca cgtcgagcgc atggcggtgc tcggcatcgc
      241 gccgcgcatc gagataggcg aatatgtcgg caccgcggaa gctgtagatc gattgcttcg
      301 gatcgcccac cagaaatacc ggcagaccgc tgccgccgta cactgtgcgg aagatgcggt
      361 actgcaccgg atcggtgtcc tggaactcgt cgatcagtgc tgcggtgtag cgctcgcgta
      421 tggcttgcgc gagcgcgctg ccgtgccctg cgccgagcgc gcccgcgaga ttgagcagca
      481 gatcgtcgta cgactggagc cgcagccgct ccttgcgcgc cgcaagttcg gtgttgcagt
      541 actccagtaa ttcggccttc aggatcgcga ggcgcttttg gtgcgccact tcgagtgccg
      601 cgtgcgcgcg cctcagtgct tcacacgccg catagaacgg atggcccggc ggattcgcgc
      661 cgcgcttcgt cgcctcggcg agcgcggctg gcgtgaattt ctcgaatcgt tcgaaaagtg
      721 tgattccggg ccgctcgggg ctgaggcacc ggtccatctc ttcgagccac tccctgacgc
      781 gatcggcgcg gtacctgcca ccgtgaaggt tacggttccc cgcgagctcc gccgcgatcg
      841 catcgtgcga ctcgcgccat atcgcccggg cgcgcgcgta ggcttgctcg tattccgatt
      901 cgaccgagtc gaggtcgccc gagagcacgg gcctgcgcac ttcgaggtac ggccacagcc
      961 tgccgttcaa gtcctcgcgc agcttgagcg gtgtcaggtt gctctcgagg acgcagcgca
     1021 cgaagagggg tgaggcaccg tagagcgtcc ggcgccagaa atcttcgacg atttcctgca
     1081 gcagcaggct ttcgtccgga agaatgcggg tctcgaacgg catcgcactt tcgaaggcac
     1141 gatccgcaag cacgcgttgg cagaaggcgt ggatggtata gacgggcgcc tggtcgaaat
     1201 cggcaagcgc acgttcgagc tggagtgccg cgcgatcgtg atccgcagcg cgctgcagca
     1261 gcccgtctag cagcggatcg tcatggcggc aagagcccga ttccagtgcc cgcgctccgc
     1321 gctcgaaggc tgcacgcgcc tcggccagcc gcgtgcggat gcgatcgcgc agctcggctg
     1381 tcgcgatttc agtaaaggtg acgaccagta tcgaatcgac gcttcgttcg gtctcgagga
     1441 tgaggcgcag gtagagcgcg gtgatcgtcc aggtctttcc cgtgccggcg ttcgcctcga
     1501 tgagcgaaac gccctcgagc ggcgcctcga gcggatggag tcgggcggat tgcgtcacgc
     1561 cgcaacctcc tctgtcatga ccgcgagcaa cggttcgaat actgcggcgg cgagcgcctc
     1621 gaactccgcg tcgagaggat cgcaaccgcg aaatgcaaga cggaaatagg gatcggcgtc
     1681 ttcgccccga gcgcgatcgg agagccacgc cctgcgcgca gcttcgaggt tgcgttcttt
     1741 tctcatgtag gcgtacgcgc tggccggaaa gaagtggagc gggcgcttaa gtcccgtcca
     1801 gtggagctcg aggagcgctg cgatcagcgc acgcgcctcg gatatcgggc gaaattggag
     1861 cgtcttgtcc tggagatgcc agcgggtcac ggtcgcgaca ccgacgggtc gcagcgcgtt
     1921 cagcacgagg tgccggaccc atgcatcgag cagatccttc ggcttgcatt ccgcgaagcg
     1981 atacccgacg agcccttcgg ggctcgctcc gctcaacgcg ccgcgaagca tcatcccgcc
     2041 gctttcgaga tcgaacgcga tcgggtcgag ccgcgctttg ggccatgcct gctcgaggcg
     2101 cccggcaaac tcgtccaccg cgcgagccgt acgctcgaat acgagcgcgc cgacctgggc
     2161 atggggcagc aatccactcg cccgggcgag ggaaagcgcg ctgcccaccg gctttccgtg
     2221 catgcgcacc ccgagcagcc gctgcttgag cgcgtagtct tccagcccgt gaagcgtgag
     2281 gggctcgcgc gcttccatct cttcgtccgc ttcctcgagc cggatgccga gccgctcgcg
     2341 gagaaaatag cgtgcggggt tgcgcagaaa gcggatgagg gactccattt ccacggtgcg
     2401 ccgcccctcc tccggttccg gcagcggttg cgacatgaac ggctttccac ccgcctcacc
     2461 tcgtttgagc tggcttgccg ccgcgcacag cgcgtgcgag tagctgaaca gtcgggggtc
     2521 cccgtagaaa tagcgcgggc tgaagggctg cagcggatga tgcgtcgtca cgtgttcgcg
     2581 aatgtcgccg cccccgggtc cggcataatt ccgtgcgaga tagtcgccga gctcgctcac
     2641 cagcacggag ggcggaatca cgctgttgtc gcggatgtgc tgaccggtat agctcacgta
     2701 aaaacagcgt cgcgccgaga tcagcgattc gagaaacaga tagcggtcgt cgtcgcggcg
     2761 cgagcggtcg cctttacgaa aatcccgcgt catcagatcg aatccgtgcg ggcggcgcat
     2821 gcgcgggaaa gcagtgtcat tcatgccgag cagacagacc acttcgaacg ggaggctacg
     2881 catcggcacc attgcgcaga acgcaacgcc gcccgagagg aacgcgcggc ccggcacttc
     2941 caggcggctg cgcagcgcgc tcttcatcac ttcgagcggg atggccgctt ggaagccgcc
     3001 tgccgcggca ttcgattcga gcgcgccgat tgccgagcgc agcgcctcga tttccgcttc
     3061 tcgctgctcg tcggggtcga agaactgtgc gaggatcgtc cgcagcgtat gggcccactg
     3121 tgacacggtg cattgcgcgg gcagcttgtt caaggcgatc gccgcctccg catagctcgc
     3181 gaaacgcccg agaatctcgc cgagcccgcc ttccacttca tcgtgcggca ggatatcgcc
     3241 gaagaggcgt tcgccgcgcc cgggaagcgc gtaacccacg atgagccgct ccaggccgaa
     3301 gcgccatgtg tgctcggcgg tcgccggcac ggaaaacgtc gcgcgatgcg cggcatcgat
     3361 gccccaccgg atcccgctct cccgcaccca gtcgtgcacg gtatcgaggt cttcggcacc
     3421 gatcgcaaag cggcgccgga cagcaggctc ttcgaggatt tcgagcacct tgccggcttc
     3481 atagcggctg ccgggaagat cgaggagcgc
//
LOCUS       DASURG010000543         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1317690, whole genome shotgun sequence.
ACCESSION   DASURG010000543 DASURG010000000
VERSION     DASURG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317690"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFY80_09755"
     CDS             complement(<1..894)
                     /locus_tag="VFY80_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6008238.1"
                     /translation="MDASEQKRVKTLTGARALVDALVREGVDHVFGIPGTQNLAVLDA
                     LRDTPQVRFILTRHEQGAAFMAYGYARAAKRPAIVTATEGPGVTNLVTAIGAAYRGFV
                     PVISVCGVQESSMRERDATQDMDQVPFLKPITKWAYSIPNPQKVQESVRKAFRVALTD
                     PQGPTHIEAASEILLEPVAPEAIEPAAYRNTVLPACDSGQLDRAFDLLSRAERPVFVV
                     GRGVLNEAATAAMAALAERTGIPVAALQYSPDAFPTTHALALGPLGRNGYGSANRTVP
                     KADLIVAVGAHFDVFSTMYKYG"
     gene            complement(912..>2073)
                     /locus_tag="VFY80_09760"
     CDS             complement(912..>2073)
                     /locus_tag="VFY80_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008239.1"
                     /translation="NQSISNLRGASTRTAKSGRLLSFNTFKGELMGTTHKILIMGASY
                     GSLLAAKILPAGHTVKLICLPVEADLINAEGIRVRLPVKGREGLYEIDSRKLPGKLSA
                     DGPGVIRDGDYDLVALAMQEPQYRSPGVREMLDAVAKAKVPCMSIMNMPPLPYLKRIP
                     GLDTEPCRAAYTDPTVWDSFDPAYLTLCSPDPQAFRPPEEKVNVLQVRLPTNFKAARF
                     ESNAHTAILRQLETDIEEARFDDHGNRIELPVKLKVHDSIFVPLAKWPMLIAGNYRCV
                     TKGGMRTIREAVHSDIEASRAVYNWVVKLCTTLGAAEKDLVPFEKYANAALSLQSPSS
                     AARALFGGAPNIERVDRLVKIIGAQKGMKLGVLDETVALVDERLEMNRKKTS"
BASE COUNT          335 a          662 c          688 g          388 t
ORIGIN      
        1 gccgtacttg tacatcgtcg agaacacgtc gaaatgcgcg ccgaccgcga cgatgagatc
       61 ggccttgggc acggtgcggt tggcgctgcc gtagccgtta cgcccgagcg ggcccaacgc
      121 cagagcgtgg gtcgtgggaa aagcatcggg cgagtactgc aatgccgcga ccggaatgcc
      181 ggtgcgctcg gcaagcgcgg ccatcgcggc ggtcgctgct tcgttcagca ccccgcgccc
      241 gaccacgaac accggccgct ccgcacgcga gagcagatcg aacgcgcggt cgagctggcc
      301 gctgtcgcag gcgggaagaa ccgtgttgcg atacgccgcg ggctcgatcg cctcgggcgc
      361 gaccggctcg agcagaatct cgctggcggc ctcgatgtgg gtagggccct gcggatcggt
      421 gagcgccacg cgaaacgcct tccgcaccga ttcctgcacc ttctgcgggt tcgggatgct
      481 gtaggcccac ttggtgatgg gcttgaggaa cggcacctga tccatgtcct gcgtcgcgtc
      541 gcgctcgcgc atcgagcttt cctgcacgcc gcacacgctg atcacgggaa cgaagccgcg
      601 gtaggcggcg ccgatcgcgg tgacgaggtt tgtcacgccg ggcccttcgg tggcggtgac
      661 gatcgcaggg cgtttcgccg cgcgcgcata gccatacgcc atgaaggcgg caccctgctc
      721 gtgccgggtg agtatgaatc gaacctgcgg cgtgtcgcgc aaggcgtcga gcaccgcgag
      781 gttctgcgtt cccggtatgc cgaagacgtg gtccacgccc tctcgcacaa gcgcgtcgac
      841 gagcgcgcgc gcaccggtca gggttttcac ccgcttctgt tcgcttgcat ccattcgttc
      901 ttggccgccc gtcagctcgt cttcttgcgg ttcatctcga ggcgctcatc gacgagcgcc
      961 acggtttcgt ccagcacgcc gagcttcatg cccttctgcg cgccgatgat cttcaccagg
     1021 cggtccaccc gctcgatgtt cggcgccccg ccaaagaggg cgcgcgcggc cgacgacggg
     1081 ctttgcagcg acagcgcggc gttcgcatac ttctcgaacg gcaccaggtc cttctccgcg
     1141 gctccgagcg tggtgcagag cttgaccacc cagttgtaga ccgcgcgcga agcttcgatg
     1201 tccgagtgca ccgcctcgcg aatggtgcgc atgcctcctt tcgtgacgca gcgatagttg
     1261 cccgcgatca gcatcggcca cttcgcgagc gggacgaaga tcgaatcgtg caccttgagc
     1321 ttcaccggca gctcgatccg gttgccgtgg tcgtcgaagc gcgcttcctc gatatcggtt
     1381 tcgagttgac gcaggatggc ggtgtgcgcg ttggattcga agcgcgccgc cttgaaattg
     1441 gtcggcagcc tcacttgcag cacgttcacc ttttcttccg gcggccggaa cgcctgcggg
     1501 tcgggactgc acaaggtgag atacgcggga tcgaagctgt cccagacggt gggatcggta
     1561 tacgccgcgc ggcagggctc ggtatcgagg ccggggatgc gcttcaggta aggcaacggc
     1621 ggcatgttca tgatcgacat gcagggcacc ttcgccttgg cgaccgcgtc gagcatctcg
     1681 cgcacgccag gagagcggta ctgcggctcc tgcatcgcga gcgccacgag atcgtaatcg
     1741 ccgtccctga tgactccggg gccgtccgca gagagcttgc cgggaagttt cctcgagtcg
     1801 atttcgtaga gcccttcccg gcccttgacc gggagacgca cgcgtatgcc ttccgcgttg
     1861 atcaggtccg cctcgacggg caggcagatc agtttcacgg tatggccggc gggcagtatc
     1921 ttggctgcaa gcagcgagcc gtaagacgcg cccatgatca gaatcttgtg ggtagtgccc
     1981 atgagttcac ccttgaacgt gttgaatgaa aggagacgcc ccgatttcgc ggtgcgcgta
     2041 gaggcgccgc gtagatttga gatgctctga ttt
//
LOCUS       DASURG010000544         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_398474, whole genome shotgun sequence.
ACCESSION   DASURG010000544 DASURG010000000
VERSION     DASURG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398474"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /gene="thiC"
                     /locus_tag="VFY80_09765"
     CDS             <1..1843
                     /gene="thiC"
                     /locus_tag="VFY80_09765"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX6008240.1"
                     /translation="AARVDEAAIQPLPNSRKVYIEGSRPEIRVPMREIRQSDTPASFG
                     AEKNPPICVYDTSGPYTDPAATIDIRSGLPPLRATWIEERNDTEVLGGPTSRYGLERL
                     ADPKLASLRFNLKRKPRRARPGFNVTQMHYARRGIITPEMEFIAIRENQRREGLASLL
                     TKQHAGESFGAALRAQITPEFVRDEVARGRAIIPSNINHPESEPMVIGRNFLVKINAN
                     IGNSAVSSGIQEEVEKMTWATRWGADTVMDLSTGKHIHETREWIVRNSPVPIGTVPIY
                     QALEKVDGKAEELTWEIYRDTLIEQCEQGVDYFTVHAGVRLPYIPLTAKRMTGIVSRG
                     GSIMAKWCLAHHTESFLYTHFEEICEILKAYDVAFSLGDGLRPGSIYDANDEAQIAEL
                     KTLGELTDIAWKHDVQVMIEGPGHVPMQLIRENMELQLKYCKEAPFYTLGPLTTDIAP
                     GYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKEDVKDGIMAYKIAAHAADLAKG
                     HPGAQVRDNALSKARFEFRWEDQFNLGLDPDKARQFHDETLPQEGAKLAHFCSMCGPH
                     FCSMRITQDVRDYAAQQGLADEEALKKGLEEKSREFLEQGGEIYNKA"
     gene            1884..>2178
                     /locus_tag="VFY80_09770"
     CDS             1884..>2178
                     /locus_tag="VFY80_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008241.1"
                     /translation="MKFILAALALGCSAMSWASEPAHEGRHPDGVADSAIREGDVDLL
                     FSYLRSVIVAAAEGREREPPPPELEQRAAEIGAELKTRGTLAALLMLSTLERQA"
BASE COUNT          449 a          671 c          703 g          355 t
ORIGIN      
        1 cgccgcgcgt gtcgatgaag ctgcgatcca gccgctgccg aattcacgca aagtctatat
       61 cgagggctcg cggcccgaaa tccgggtgcc gatgcgcgag atccggcaat ccgatacgcc
      121 ggcctcgttc ggcgcggaga agaatccgcc gatctgcgtc tatgacactt ccggacccta
      181 cactgatccg gcggcaacga tcgacatccg ttccggattg cctccgctgc gcgcgacgtg
      241 gatcgaggag cgcaacgaca cggaagtgct gggggggccg acttcgcgct acggccttga
      301 gcggctcgcc gacccgaagc tcgcgagcct gcgctttaac ctgaagcgca agccgcggcg
      361 cgcacggccc ggcttcaacg tgacgcagat gcattacgca cgccgcggca tcatcacgcc
      421 ggaaatggag ttcatcgcta tccgcgaaaa tcagcggcgc gaagggctcg cctcactcct
      481 gacgaaacag cacgccggcg aatcgttcgg tgcggcgctc cgcgcgcaaa tcacgccgga
      541 attcgtgcgc gacgaagtgg cgcgcggacg ggccatcatt ccctcgaaca tcaaccaccc
      601 cgaaagcgag ccgatggtga ttggccgcaa cttcctcgtg aagatcaacg cgaatatcgg
      661 caattccgcg gtgagttccg ggatccagga ggaagtggag aagatgacct gggcgacgcg
      721 ctggggcgcg gacaccgtca tggatctctc caccggcaag cacatccatg agacgcgcga
      781 gtggatcgtg cgcaattcgc cggttccgat cggcacggtt ccgatctacc aggcgctcga
      841 gaaggtggac ggaaaagccg aggagctcac ctgggagatc taccgcgaca cgctgatcga
      901 gcagtgcgag cagggcgtcg attactttac ggtgcacgcc ggcgtgcgcc tgccttacat
      961 cccgctcacc gcgaaacgca tgactggcat cgtctcgcgc ggcgggtcga tcatggcgaa
     1021 gtggtgcctt gcgcatcaca cggagagctt cctctacacg catttcgagg aaatctgcga
     1081 gatcctcaag gcgtacgacg tcgcgttttc gctgggcgac ggattgcggc ccgggtccat
     1141 ctacgatgcg aatgacgaag cgcagatcgc cgagctgaag acattgggag agctcaccga
     1201 tatcgcgtgg aagcacgatg tccaggtgat gatcgaagga ccgggtcacg tgccgatgca
     1261 gctcatccgc gagaacatgg agctccagct caagtactgc aaggaagcgc cgttctacac
     1321 gctgggaccg ctcacgaccg atatcgcgcc cggctacgat cacatcacct cggcgatcgg
     1381 cgcggcgatg atcggatggt acggcaccgc tatgctgtgt tacgtgacgc ccaaggagca
     1441 tctgggcctg cccgacaagg aggatgtgaa agacggcatc atggcctaca agatcgccgc
     1501 ccatgccgcc gatctcgcca aggggcatcc gggcgcgcag gtccgcgaca atgcgctctc
     1561 gaaggcgcgc ttcgagttcc gctgggagga ccagttcaac ctgggtctcg atcccgacaa
     1621 ggcgcggcaa ttccacgacg agacgctgcc gcaggagggc gccaagctcg cgcacttctg
     1681 ctcgatgtgc gggccgcatt tctgctcgat gaggatcaca caggacgtgc gcgactatgc
     1741 ggcacagcag gggctcgctg acgaagaggc gctgaagaag gggctcgaag agaagtcgcg
     1801 cgagttcctc gaacagggcg gcgaaatcta taacaaggcg tagtcaacgc ttttctcaag
     1861 gtcgggaacg ctcgatgcgc catttgaaat tcattctcgc agcgctggca ttgggctgca
     1921 gcgcgatgtc atgggccagt gaaccggctc acgaggggcg ccacccggac ggcgtggctg
     1981 acagcgcgat tcgggaaggc gatgtagatc tgctcttcag ctacctgcgc tccgtgatcg
     2041 ttgcggcggc ggaaggacgg gagcgcgagc cgccaccccc ggaactagag cagcgagcgg
     2101 ctgagatcgg cgcggaactt aagacccgcg ggacgctcgc tgcgctcctc atgctttcga
     2161 cactggaaag gcaggcca
//
LOCUS       DASURG010000545         7669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_500340, whole genome shotgun sequence.
ACCESSION   DASURG010000545 DASURG010000000
VERSION     DASURG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500340"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /gene="murC"
                     /locus_tag="VFY80_09775"
     CDS             <1..1181
                     /gene="murC"
                     /locus_tag="VFY80_09775"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX6008242.1"
                     /translation="PEVLAARAKSVPVVPRALMLAELMRLKQGIAVAGTHGKTTTTSL
                     IASLLAEGGMDPTFVIGGRLEAAGSHAKLGQGEFIVAEADESDASFLYLQPTLAVVTN
                     IDADHMATYEHSLDRLKQAFVDFVQRLPFYGVVVLCCDDPNVREIMPRLTKPMVTYGF
                     SEDAEIRAVDVGAAGCRMKFRVVRKGNGRKPPDLLITLNLAGTHNVQNALAAIAVGME
                     LGIPDASLVKALGEFRGVGRRFERYGEIPLPAGGSFSLIDDYGHHPAEIAATLAAVRG
                     AFPGRRIVLAFQPHRYTRTRDCFEDFAKVLSRADAVVLTEVYSAGEAPIVAADGRALA
                     RAVRVLGRVEPIFVESVAELPQAILAAARDGDVVVTMGAGAIGGVPALLKRSKEENEK
                     "
     gene            1178..2200
                     /gene="murB"
                     /locus_tag="VFY80_09780"
     CDS             1178..2200
                     /gene="murB"
                     /locus_tag="VFY80_09780"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX6008243.1"
                     /translation="MRTDTITCAVRGSRSGSAHLVRLAAPRANGGEAFEMAARRELRG
                     KLHYNEPMSRHVSWRAGGIADRAYEPADLSDLQVFMQTMPAHEPVHFLGLGSNLLVRD
                     GGLRATVILTHWAMRAIRLENDGHRIYAEAGLPSPKVARFAALHGLVGAEFLAGIPGT
                     VGGALAMNAGCYGGETWDLVERVFTINRSGEVHERHPGEYQVAYRSVARRSVAEGEVD
                     AEWFVAAVFRLVPGNGEVSRQRIKELLARRIATQPLGEPNAGSVFRNPRGDFAARLIE
                     ACGLKGYTIGDAMISPKHANFIVNRGKASAADIEALIERAQGAVRERFGIDLEREVRI
                     IGEYAA"
     gene            2197..3108
                     /locus_tag="VFY80_09785"
     CDS             2197..3108
                     /locus_tag="VFY80_09785"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX6008244.1"
                     /translation="MSRFGKVAVLMGGRSAEREISLKSGGMVLAALNSKGIDAHPFDP
                     RDAGLEQLIAQKFDRVFIALHGRFGEDGTVQGALEYLGIPYTGSGVMASALAMDKWRT
                     KLLWQAAGVPTPPYELLTAASDFVGVAARIGLPLMVKPAREGSSIGMSKVESVEKIAP
                     AYALATHYDDVILAEHYIQGIELTAAILGDEALPLIRLETPRVFYDYQAKYFADDTRY
                     ICPCGLPQPQEQRIQADALRAFRLIGCSGWGRVDVMLDPSGQPYLLEVNTIPGMTDHS
                     LVPMAARARGIAFEDLVVRILESAHVG"
     gene            3098..3844
                     /locus_tag="VFY80_09790"
     CDS             3098..3844
                     /locus_tag="VFY80_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX6008245.1"
                     /translation="MWDRPDALNRSADLLLAVALLLALYGAFHVVVRLPLFPLREVRV
                     MHPLAYVKPGQIETLIRREVHGNFFTVDIAAVRAAFVKLPWVREASLRRAWPDRLEVT
                     LEEHVPLARWGESGLLNTQGEVFNAAYDGNLPVFAGPPESAKEMAIQYEHFRRSLETI
                     GKVPQQLRVTARRAWQVKLEDGMTLELGREEVEPRLARFIAAYRLTLAPLTRKVEVVD
                     LRYGNGFAVRIPELRHDKGVPKGRRSTSQG"
     gene            3849..5087
                     /gene="ftsA"
                     /locus_tag="VFY80_09795"
     CDS             3849..5087
                     /gene="ftsA"
                     /locus_tag="VFY80_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX6008246.1"
                     /translation="MGKSRENTRLIVGLDVGTSKIAAIAAEIKPEGGFEIIGMGSHPS
                     RGLKKGVVVNIETTVSAIQRALEEAELMADCKIREVYTGIAGSHIKSFNSQGMVAIKD
                     KEVAQMDIDRVIETAKAVQIPNDQQILHILNQEFIIDGQEDVREPLGMSGVRLEAKVH
                     IVTGAVSAAQNIIKCVRRCGLEVKDLILQPLASALAVITEDEKDLGVCLVDIGGGTTD
                     VAVFTHGAIRHTAVIPIAGDQITNDIAMALRTPTKDAEDIKQRFGCALSQLADPQEMV
                     EVPGVGDRGPRELSRKTLAEVIEPRVEELYSLVQAELRRSGYEELLSSGIVLTGGSCV
                     LQGMVELGEEIFHMPVRIGKPNYSGGLAEVVRHPRYSTGVGLLLAGVQQYRSRELARL
                     QVGPVQQAMERMRSWFSGNF"
     gene            5155..6315
                     /gene="ftsZ"
                     /locus_tag="VFY80_09800"
     CDS             5155..6315
                     /gene="ftsZ"
                     /locus_tag="VFY80_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX6008247.1"
                     /translation="MIELIDTQSQEAVIKVIGVGGCGGNAIDHMIARGVQGVEFICAN
                     TDAQALKRNQAKVQLQLGTGITKGLGAGANPEIGRQAALEDRERIAELITGADMLFLT
                     AGMGGGTGTGAAPIVAEVAKELGVLTVAVVTKPFAFEGKRQRIAQEGLDALAQHVDSL
                     IVIPNDKLMQVLGSQVTLDEAFQAANEVLHGAVAGIAEIISCPGMINVDFADVRTVMG
                     EMGMAMMGSAKADGPDRARVAAEQAVACPLLEDINISDARGVLVNISASKTTFQLQEM
                     YDVMETIKAFAADSATVIVGTVYDDALESNLRVTIVATGLGKPAARHQAKPLSVVAQK
                     TGTDNLGIDVDYSALEQPAVIRRRNRDATIEAMRQSGVEMLDIPAFLRKQAD"
     gene            6476..7387
                     /gene="lpxC"
                     /locus_tag="VFY80_09805"
     CDS             6476..7387
                     /gene="lpxC"
                     /locus_tag="VFY80_09805"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX6008248.1"
                     /translation="MIRQRTLKNTVRATGVGLHTGLKVALTLRPAAANTGIVFRRVDL
                     DAPVEIKANPYAVGDTRLSSCLEKDGVRVSTVEHLMSAFGGLGIDNAYVDVSAPEVPI
                     MDGSAGPLVFLLQSAGVEEQNVPKKFIRILQPVEVQEGDKWVRFEPHNGFKLELAIDF
                     DHPVFDRARQSVCVDFATTSYIKEVSRARTFGFMHDVESMRAQGLALGGSLDNAIVMD
                     DYRVLNTDGLRYSDEFVKHKVLDAIGDLYLLGHPLIGAFSGYKSGHALNNLLLRRLLE
                     TQRAWDYASFERTEDAPRFLGWQTQFA"
     gene            7482..7667
                     /locus_tag="VFY80_09810"
     CDS             7482..7667
                     /locus_tag="VFY80_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008249.1"
                     /translation="MWLLRLVGALALIAIGATLLLYLFTRNRRYLTVAWRVFQFALVF
                     AVVVMLFYVVERLLLVL"
BASE COUNT         1426 a         2333 c         2554 g         1356 t
ORIGIN      
        1 acccggaagt gctcgccgca cgagcaaaga gcgtgccggt ggttccccgc gcgctgatgc
       61 ttgccgaact catgcgactc aagcagggca ttgcggtcgc gggcacacac gggaaaacca
      121 cgacgaccag tctcatcgcg agcctgctgg cggaaggcgg catggatccc acgttcgtca
      181 tcggtggccg gctggaggcg gcgggttcgc acgccaagct cggccagggt gagttcatcg
      241 tggccgaggc ggacgagtcc gatgcgtcct ttctctacct gcaaccgacg ctcgcagtgg
      301 tcaccaacat cgatgccgat cacatggcga cctacgagca ctcgctcgac aggctgaagc
      361 aggcgttcgt cgacttcgtg cagcgcctgc cgttttacgg cgtggtcgtg ctgtgttgcg
      421 acgacccgaa cgtgcgcgag atcatgccgc ggctcacgaa gcccatggtg acctacggat
      481 tttccgagga cgccgagatc cgcgcggtgg atgtcggagc cgcggggtgc cggatgaagt
      541 ttcgcgttgt gcgcaagggg aacggtcgaa agccgcccga ccttctcatc acgctcaacc
      601 tcgccggaac gcacaacgtg cagaacgcac ttgcggcgat tgccgtggga atggagctcg
      661 ggattcccga tgcgagcctc gtcaaagccc tcggcgaatt ccggggtgtg ggtcggcgct
      721 tcgagcgcta cggcgaaatc ccgttgcccg ccggcggctc gttcagcctg atcgacgatt
      781 acggacacca tcccgccgaa atcgctgcga cgcttgcggc ggtccgcggc gcgtttcccg
      841 gccggcgcat cgtgctcgcg tttcagccgc atcgctacac ccgtacgcgc gattgcttcg
      901 aggatttcgc gaaagtgctc tccagggcgg atgcagtcgt gctgaccgag gtttattccg
      961 ctggcgaagc gcccatcgtc gccgccgatg gccgggcact tgcccgtgcc gtgcgcgtgc
     1021 tgggcagggt cgagccgata ttcgtcgagt ccgtcgccga attgccgcaa gccatattgg
     1081 cggcggcacg cgacggggac gtcgtcgtca ccatgggcgc gggcgccatc ggaggcgtgc
     1141 ccgcgctgct caagcgttcg aaagaagaga atgaaaaatg aggaccgata cgatcacctg
     1201 cgcggtgcga ggctcgcgaa gcggcagcgc tcacctcgtc cgcctggctg caccgcgtgc
     1261 gaatggaggc gaagcgttcg agatggcggc gcgacgtgag ctgagaggca agctccacta
     1321 caacgagccg atgtcccggc acgtgagctg gcgcgccggc ggcatcgccg atcgcgcgta
     1381 cgagcctgcg gacctttctg atctgcaggt tttcatgcag acgatgccgg cgcacgagcc
     1441 agtgcatttt ctcggcctcg gcagcaatct cctcgtgcgc gacggggggc ttcgcgccac
     1501 ggtcatcctc acccactggg ccatgcgtgc gatcaggctc gaaaacgacg gccatcgcat
     1561 ctacgccgag gcaggtctcc cgagccccaa agttgctcgc ttcgcagcgt tacacgggct
     1621 ggtcggcgcc gaattcctcg ccggcattcc gggcacggtc ggcggagcgc tcgccatgaa
     1681 tgccggctgc tatggcggcg aaacgtggga cctcgtggag cgcgtattca caatcaatcg
     1741 cagcggtgaa gttcacgagc ggcaccccgg tgaataccag gtggcgtacc gctctgtggc
     1801 gcggaggtcc gttgcagaag gcgaagtgga cgccgagtgg ttcgttgcag cggtgttccg
     1861 gttggtaccc ggaaacggcg aagtgtcccg acagcgcatc aaggagctgc tcgcgcggcg
     1921 catcgcgacc caaccgctcg gcgaaccgaa cgcaggctcg gtgttccgta atccgcgggg
     1981 cgacttcgcg gcacggttga tcgaggcgtg cggcctcaaa gggtacacga tcggcgacgc
     2041 gatgatttcg ccgaagcacg ccaacttcat cgtcaatcgc ggcaaggcga gcgcggccga
     2101 tatcgaagcg ctcatcgagc gcgcgcaggg ggccgtgcgc gagcgcttcg gcatcgatct
     2161 cgaacgcgag gtgcgcatca tcggggagta cgctgcatga gtcgcttcgg aaaagttgca
     2221 gtactaatgg gcggccggtc ggccgaacgg gaaatctcgc tcaagagcgg cggcatggtg
     2281 cttgcggcgc tgaacagcaa agggatcgat gcccacccct tcgacccgcg cgatgcgggg
     2341 cttgagcagc tcatcgcgca gaagttcgac cgggtgttca tcgcgctcca cgggcgcttc
     2401 ggagaggacg gcacggtgca gggagcgctg gaataccttg gcattccgta cacgggcagc
     2461 ggcgtcatgg cgagcgcact cgcgatggac aagtggcgca ccaagctgct gtggcaggcc
     2521 gcgggcgtgc ccacaccgcc ctacgagctg ctgacggcag cgagcgactt tgtgggtgtg
     2581 gcggcgcgga tcggcctgcc gctcatggtg aagcccgcgc gcgagggatc gagcatcggc
     2641 atgagcaaag tggaatcggt ggagaaaatc gcgccggcgt acgcgcttgc cacgcactat
     2701 gacgacgtga ttctcgccga gcactacata cagggcatcg agctcactgc tgcgatcctc
     2761 ggggacgaag cgctgccgct cattcgtctg gagaccccgc gcgtcttcta cgactaccag
     2821 gcgaagtact ttgctgacga cacccgttac atctgcccct gcggtctgcc gcagccgcag
     2881 gagcagcgca tacaggccga tgcgctgcgt gctttccgcc tcatcggatg cagcggctgg
     2941 ggccgcgtcg acgtgatgct ggatccttcc gggcagccgt atctcctcga agtcaacacg
     3001 attcccggaa tgacggatca cagcctcgtg ccgatggccg cccgcgctcg cggcatcgca
     3061 ttcgaagatc tggtcgtccg catattggag tccgcgcatg tgggataggc ccgacgcgct
     3121 caaccgctca gcggatctgc tgctcgctgt ggcgttgttg ctcgcgcttt acggcgcttt
     3181 ccacgtcgtg gtcagacttc ctctgtttcc gctgcgcgag gtgcgcgtca tgcaccctct
     3241 tgcatacgta aaacccgggc agatcgaaac gctgatccgt cgcgaagtgc acggcaattt
     3301 tttcaccgtc gatatcgcgg ccgtgcgtgc cgctttcgtg aagctcccat gggtgcggga
     3361 ggcgagcctg cgccgcgcct ggcctgaccg gctcgaggtg acgctggaag aacacgtgcc
     3421 gctcgcgcgc tggggcgaga gcgggctact caacacgcaa ggtgaagtgt tcaacgccgc
     3481 atacgacgga aacctgcccg tctttgccgg accgcccgaa tcggccaagg agatggcgat
     3541 ccaatacgag catttccggc gcagcctgga gacgatcgga aaggtgccgc agcagctccg
     3601 ggtgacggcg cggcgcgcct ggcaggtgaa actcgaggac ggcatgactc tggaactcgg
     3661 gcgcgaggag gtcgagccgc ggcttgcgcg tttcatcgcg gcatatcgcc ttacgctcgc
     3721 gccgctcacg cgcaaagtgg aagtcgtcga tctgcggtat ggcaacggct ttgcggtacg
     3781 cattccggaa ttgcggcatg acaaaggcgt tccgaaggga cgcagaagta cgtcacaggg
     3841 gtagggatat gggcaagtca agagagaaca ctcgcctcat cgtgggactg gatgtcggca
     3901 cgtccaagat cgcggcgatc gccgccgaga tcaagccgga aggcggattc gaaatcatcg
     3961 gcatgggaag ccatccatcg cgcggactca agaaaggggt cgtggtcaac atcgaaacga
     4021 ccgtgagcgc gattcagcgc gcgctcgaag aggcggagct catggccgac tgcaagatcc
     4081 gcgaagtcta taccggcatt gcgggcagcc acatcaaaag cttcaactcg caggggatgg
     4141 tggcgatcaa ggacaaggaa gtggcccaga tggatatcga tcgcgtgatc gagaccgcaa
     4201 aggccgtgca gattcccaac gaccagcaga tcctgcacat cctcaaccag gaattcatca
     4261 tcgacggcca ggaggacgtg cgcgagccgc tcggcatgtc gggcgtgcgg ctcgaagcaa
     4321 aggtgcacat cgtgacgggt gccgtttcgg ccgcgcagaa catcatcaag tgcgtgcgcc
     4381 gctgcgggct cgaggtgaag gatctcatcc tgcagccgct tgcctccgcg ctcgccgtca
     4441 tcacagagga cgagaaggac ctcggcgtat gcctcgtcga catcggcggc ggcacgaccg
     4501 atgtcgccgt attcacccat ggagcgatcc gccacaccgc ggtcatcccg attgcgggcg
     4561 atcagatcac gaacgatatc gcgatggcgc tcaggacgcc aaccaaggat gcggaggaca
     4621 tcaagcagcg ctttggctgc gcgctctccc agctcgccga tccgcaggaa atggtggaag
     4681 tgccgggcgt gggcgatcgc gggccgcggg agctttcgcg caagacgctc gccgaagtca
     4741 tcgagccgcg cgtcgaggag ctctattcgc tagtgcaggc ggaactgcgc cgcagcggct
     4801 acgaggagct gctctcctcg gggattgtgc tgaccggtgg ctcgtgcgtg ttgcagggca
     4861 tggtcgagct gggcgaggag atctttcaca tgccggtgcg catcggcaag ccgaattaca
     4921 gcgggggact cgcggaggtg gtgcgtcacc cgcgctattc aaccggcgtc gggctcttgc
     4981 tcgcgggcgt ccagcaatat cgcagccgcg agctggcacg cctgcaggtg gggcccgtcc
     5041 agcaggcgat ggaacgcatg cgcagctggt tttcgggaaa tttctgatgc gggcagtaga
     5101 ttgaagggcg atcggagtcg gatgcaccac gtcacatacc acaaggagaa ctgcatgata
     5161 gagctcatcg atacgcaatc ccaggaagcg gtcatcaagg tcatcggcgt cggcggctgt
     5221 ggcggcaatg ccatcgatca catgattgcg cgcggcgtgc agggggtgga gttcatctgc
     5281 gccaatacgg acgcgcaggc gctcaagcgc aatcaggcca aggtgcagct gcagctcggc
     5341 accggcatca cgaagggatt gggtgccggg gccaatcccg agatcggccg ccaggcggcg
     5401 ctcgaagacc gcgaacgcat agcggagctc atcactggcg cggacatgct gttcctcacg
     5461 gccggcatgg gcggcggcac cgggacgggt gctgcgccca ttgtcgcgga agtcgccaag
     5521 gagctcggcg tgctgaccgt cgccgtggtg accaagccgt tcgcgttcga aggcaagcgt
     5581 cagcgcatcg cgcaggaagg gctcgatgcg ctcgcccagc acgtcgattc gctcatcgtg
     5641 attcccaacg acaaactgat gcaggtcttg ggaagccagg tcacgctcga tgaggcgttt
     5701 caggcggcaa acgaagtact gcacggagcg gtggctggca tcgcggagat catcagctgt
     5761 ccggggatga tcaacgtcga tttcgcggac gtccgcacgg ttatggggga aatgggcatg
     5821 gcgatgatgg gctcggcgaa ggccgatggt cccgatcgcg cccgcgtggc ggcggagcag
     5881 gcggtcgcgt gcccgctgct cgaagacatc aacatatcgg acgcgcgcgg cgtactcgtc
     5941 aacatttccg cgagcaagac gacgttccag ctccaggaaa tgtacgatgt gatggaaacg
     6001 atcaaggcgt ttgccgcgga ctcggccaca gtgatcgtcg gcacggtgta tgacgatgcc
     6061 ctggaatcga acctgcgggt caccatcgtt gccacggggc tcggcaagcc tgcggcccgg
     6121 caccaggcga agccgctttc cgtggtcgcc cagaagacag gtacggacaa tttgggaatc
     6181 gacgtggact acagcgcgct cgagcagccc gccgtcatcc gccgccgcaa tcgcgatgcc
     6241 acgatagagg ccatgcgtca gtcaggcgtc gaaatgctcg acattccggc gtttctgcgc
     6301 aagcaggcgg actgatggtg acgccagatg gcaggggcga gcgggatcgc cccttggaag
     6361 cggaaacagc cgcgcgcgat atgacatgcg taacggactg taaaagtctt tgccgggcgc
     6421 gcggcatgct gttagaatcg caaggttaag gatctgatat ttcgaagact tttcgatgat
     6481 tcggcagcgc accttgaaga acaccgtccg ggcgaccggc gtcgggctgc acaccggcct
     6541 caaggtggca ctcaccctgc ggccggctgc tgccaacacc ggcatcgtgt tccgcagggt
     6601 ggatctcgac gcgccggtcg agatcaaggc gaatccctac gcagttggcg atacgcgttt
     6661 gtcatcgtgt ctcgaaaagg acggcgtgcg cgtatccacg gtagagcatt tgatgtcggc
     6721 gtttggcggt ctcggcatcg acaatgctta tgtcgatgtg tccgcccctg aagtgccgat
     6781 catggacggt agcgcgggtc cgctcgtgtt cctgctccag tcggccggcg tcgaagagca
     6841 gaacgtaccc aagaagttca tccgtatcct tcagccggtc gaagttcagg agggcgacaa
     6901 gtgggtccgt ttcgagcccc acaacggctt caagctcgag ctcgccatcg acttcgatca
     6961 ccccgtgttc gatcgcgccc ggcaatcggt atgcgttgat ttcgcgacga cctcctatat
     7021 caaagaggta agtcgtgcgc gcacgttcgg cttcatgcac gatgtggaaa gcatgcgggc
     7081 ccaggggctg gcgctggggg gcagcctcga caatgcgatc gtcatggatg actatcgggt
     7141 tctcaacacc gacgggctgc gctacagcga cgaattcgtg aagcacaagg tactggacgc
     7201 gatcggcgac ctctaccttc tcggtcatcc gctgatcggc gccttcagcg gctacaaatc
     7261 ggggcacgca ctcaacaatt tgctcctgag gcggctcctc gaaacgcaac gcgcatggga
     7321 ctacgcgagc ttcgagcgta ccgaggacgc gccccgtttc ctgggctggc agacgcagtt
     7381 cgcgtgatct cggcgctttt atggtcgacg atgggcgcgg ggcggcggcg gtgacgggac
     7441 ggtcgtcccc gcagtgaccg aactgccggc actgacgcta catgtggctc ctgcgactcg
     7501 tcggcgccct tgcgctcatc gcgatcggcg cgactctcct cctctatctt tttacccgta
     7561 accgccgtta cctgacagtg gcttggcgag tgtttcagtt cgccttggtc ttcgcggtcg
     7621 tcgtcatgct gttctacgtc gtcgagcgcc tgttacttgt tctctgagc
//
LOCUS       DASURG010000546         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1318319, whole genome shotgun sequence.
ACCESSION   DASURG010000546 DASURG010000000
VERSION     DASURG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318319"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFY80_09815"
     CDS             <1..411
                     /locus_tag="VFY80_09815"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX6008250.1"
                     /translation="VRNKSEQFEARFSLVEVVASASLFFEGMAGSRIPIAVAHGEGYA
                     EFRDAAALDAARPLIALRYVDNRGQPTEVYPANPNGSPRGITGLTTPDGRVTILMPHP
                     ERVFRSVQNSWHPDQWGEDGPWLRMFRNARKWCG"
     gene            complement(421..1203)
                     /locus_tag="VFY80_09820"
     CDS             complement(421..1203)
                     /locus_tag="VFY80_09820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6008251.1"
                     /translation="MRVSKMQQIAAALALGAVLVPAAAQGVKVNGKEIPQSRIDAGVK
                     SRIAQGQPDTPELRTNVKDALVNQEIIAQEAMKKGLHKKPDVAAAIDINRQDILVNAY
                     VQDYLEKNPVNDDTLKKEYERIKGQMGGKEYKARHILVEKEAEAKDIISQVKKGASFE
                     KLAAERSKDNGTKERGGDLDWSVPGNFVKPFGDALAKLKKGQMTDTPVQSNFGWHVIR
                     LDDERPFQAPPFDQVKQNLQRNMQQQMVQKMLGELRAKAKIE"
     gene            complement(1221..1493)
                     /locus_tag="VFY80_09825"
     CDS             complement(1221..1493)
                     /locus_tag="VFY80_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX6008252.1"
                     /translation="MRAPGTARAIEERLAALQPESLEVLDESARHVGHEGAKGGGGHY
                     TVTIVSPAFAGKPVQARHRMVYAALGPMMHKEIHALAIKAYAPDEI"
     gene            complement(1493..2032)
                     /locus_tag="VFY80_09830"
     CDS             complement(1493..2032)
                     /locus_tag="VFY80_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX6008253.1"
                     /translation="MKFLFDIFPVLLFFVAFKLYDIYVATAVAIGATFVQIGWVWLRH
                     RKVDTMLWVSLGVIVVFGGATLLLHDETFIKWKPTVLYWLFGAVLAVASLGFRKNLIR
                     ALMEKQMSLPDIAWSRLQTSWIGFFAVMGALNLFVAYNFSTDAWVNFKLFGGMGLMLV
                     FVVLQALMVGKYVEDRSGK"
     gene            complement(2153..2548)
                     /gene="msrB"
                     /locus_tag="VFY80_09835"
     CDS             complement(2153..2548)
                     /gene="msrB"
                     /locus_tag="VFY80_09835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6008254.1"
                     /translation="MTEKVVKSDAEWKAQLTPEQYQVTRRHGTEGAFSGAYWDNHEKG
                     VYRCVCCGVELFRSDHKFDSGTGWPSFWQPSAPQNVASEEDTSYFMRRTEVHCGRCQA
                     HLGHVFEDGPQPTGLRYCINSASLRFDKE"
     gene            complement(2567..4006)
                     /locus_tag="VFY80_09840"
     CDS             complement(2567..4006)
                     /locus_tag="VFY80_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6008255.1"
                     /translation="MPAHTLIERIAAIVGPQGLLTDAADVEPHVVDWRGIFRGATPAV
                     VRPANTEEVAAVVKLCAESRTPIVPQGGNTGMCGASVPHASGNEIILTLSRMSRVLEV
                     DALNNTMTVEAGCILANIQQAAADADRLFPLSLGAEGSCQIGGNLSTNAGGVNVLRYG
                     NTRDLVLGLEVVLPDGGIWNGLRGLRKDNTGYDLKQLFIGAEGTLGIITAAVLKLFPR
                     PAASATTWTAVPHPEAALEFLALLRRHCGDRVTAFEILSRNCLDLVMRHIPGARDPLP
                     KPHEWYVLAELSDSHSGERLRHELERALESAMESALVIDAVVAENEAQARALWRLRET
                     VPEAARGEPGMLYRHDISVAVSRIPAFIREAHAALGARFPGANVICFGHLGDGNLHYN
                     AFIPGRERSDAAAREAQDVTTFVYDIVHRYDGSFSAEHGIGLAKVNDMVRYKSAIELE
                     LMTAIKGTLDPHGIMNPGKVLELRSPVGL"
BASE COUNT          695 a         1334 c         1276 g          783 t
ORIGIN      
        1 gtgcgcaaca agtccgagca gttcgaagcg cgcttttcgc tggtcgaggt cgtggcgtcg
       61 gcgtcgctct tcttcgaggg catggccgga agcagaatcc cgatcgcggt ggcccacggc
      121 gagggttatg cggaatttcg cgatgccgca gcgctggacg ccgcgcggcc gctgatcgcg
      181 ctgcgctacg tggacaaccg cgggcaaccg accgaagtct atccggcgaa tccgaacggc
      241 tcaccgcgcg gcattaccgg gcttacgaca cccgacggac gggtgaccat actcatgccc
      301 catccggagc gcgtcttccg cagcgtccag aattcctggc atccggacca atggggcgaa
      361 gacgggccgt ggctcaggat gttccgcaac gcacggaagt ggtgcggctg atactcgagg
      421 ttactcgatc ttcgctttcg cgcgcagctc ccccagcatc ttctgaacca tctgctgttg
      481 catgttgcgt tgcagattct gcttcacttg atcgaacggc ggagcctgga acggccgctc
      541 gtcgtcgagt cggatcacgt gccagccgaa gttcgactgg accggcgtat cggtcatctg
      601 acctttcttg agtttcgcga gcgcgtcgcc gaagggcttc acgaaattgc ccggcacgct
      661 ccagtcgaga tcaccgcccc gctccttggt gccgttgtcc ttcgagcgct ccgcggcgag
      721 cttttcgaag ctcgcgccct ttttcacctg ggagatgatg tccttcgcct cggcttcctt
      781 ctcgacgagg atgtggcgcg ccttgtattc cttgccgccc atctgcccct tgatgcgctc
      841 atactccttc ttgagcgtat cgtcgtttac cgggttcttc tcgagatagt cctgcacata
      901 cgcgttgacg aggatgtcct ggcggttgat gtcgatcgcc gccgcgacgt cgggcttctt
      961 gtgaagaccc ttcttcatcg cctcctgcgc gatgatttcc tgattgacca gcgcgtcctt
     1021 cacgttggtg cgcagctccg gcgtatcggg ttgaccctgc gcgatacggc tcttcacgcc
     1081 cgcgtcgatg cgcgactgcg ggatctcctt gccgttcacc ttgacgccct gcgccgccgc
     1141 tggcacgagc acggcaccga gcgcaagcgc cgcggcgatc tgctgcattt tgctgaccct
     1201 catgtctttt cctttcatca tcatatttcg tccggcgcgt aggccttgat ggcgagcgcg
     1261 tggatctcct tgtgcatcat cggcccgagc gccgcgtata ccatgcgatg tcgggcctgt
     1321 accggcttgc ccgcgaacgc gggcgaaacg atcgtcaccg tgtaatgccc gccgccaccc
     1381 ttggcgcctt cgtgtccgac gtgcctggcg gattcgtcca gaacctccag cgattcgggc
     1441 tgcagcgccg ccagccgttc ctcgatcgct cgcgccgtac cgggtgccct catcacttgc
     1501 ccgagcggtc ttcgacgtac ttgccgacca tgagcgcctg tagcacgaca aacacgagca
     1561 tgagccccat gccgccgaag agcttgaagt tcacccacgc gtctgtggag aaattgtagg
     1621 cgacgaagag gttgagcgcg cccatgacgg cgaagaaccc gatccagctc gtctgcaatc
     1681 ggctccacgc aatgtccggg aggctcatct gcttttccat gagcgcacgg atcaggttct
     1741 tcctgaagcc cagcgacgcg acggcgagca cggcaccgaa gagccagtag agcacagtcg
     1801 gcttccactt gatgaacgtc tcgtcgtgga ggagcagcgt cgctccgccg aagacgacga
     1861 ttacgcccag actcacccac agcatcgtgt caaccttgcg gtggcgcagc cacacccacc
     1921 cgatctgcac gaaggtcgcc ccgatggcga ccgccgtcgc gacgtagatg tcgtagagct
     1981 tgaacgcgac gaaaaacagc aggaccggga agatgtcgaa gaggaacttc atggatggtg
     2041 cgcgtggaag agaggcgtgc ggaacgcgga acgggcgcag gacatggcgg caaatggcgc
     2101 attatcgccg tttgccggct tcacgtccat ggagggagac gagccccggc cgctattcct
     2161 tgtcgaaacg aagtgaagcc gagttgatgc agtagcgcaa tcccgtgggc tgcggaccgt
     2221 cttcgaagac gtgccccagg tgcgcttggc agcggccgca gtgcacctcg gtgcggcgca
     2281 tgaagtagct cgtgtcttct tcgctcgcga cgttttgcgg cgccgagggc tgccagaagc
     2341 tcggccagcc ggtgcccgaa tcgaatttgt gatcggagcg aaagagctcg acgccgcagc
     2401 acacgcagcg atagacgcct ttctcgtgat tgtcccagta cgcgccgctg aaggcgccct
     2461 ccgtgccgtg cctgcgcgtc acctggtact gctccggcgt caactgcgcc ttccactcgg
     2521 catcgctctt gacgactttc tccgtcatct tcgcctccac ttcgcttcag agaccgaccg
     2581 gcgatcgaag ttccagcacc ttgcccggat tcatgatgcc atgagggtcg agcgtgccct
     2641 tgatcgccgt catgagctcc agttcgatcg cgctcttgta gcggaccatg tcattcactt
     2701 tggcgaggcc gatgccgtgc tcggcgctga agctgccatc gtagcgatgc acgatatcgt
     2761 agacgaatgt cgtcacgtcc tgggcctccc gcgcggcggc gtcgctgcgc tcgcgccccg
     2821 ggatgaaggc gttgtagtgc aggttgccgt cgccgagatg accgaagcag atcacgttcg
     2881 cgcccggaaa gcgtgcgccc aatgccgcat gcgcttcgcg gatgaaagcg ggaatccggc
     2941 tgacggcgac cgagatgtcg tgacgataca gcatgccggg ctcgccgcgc gcggcctcgg
     3001 gcacggtttc gcgcagccgc cacagcgccc gcgcctgcgc ctcgttctcc gcgacgacgg
     3061 catcgatcac gagcgccgac tccattgcgg attcgagcgc gcgctcgagc tcgtggcgca
     3121 gccgctcgcc gctgtgggaa tcgctcagct cggcgagcac gtaccactcg tgcggcttcg
     3181 gaagcggatc gcgcgccccc ggaatatggc gcatgacgag atcgagacag ttgcgcgaca
     3241 ggatttcgaa cgcggtcacc cgatcgccgc aatgacggcg cagcagcgcg agaaattcga
     3301 gcgccgcttc gggatgcggc acggcggtcc acgtggtcgc gctcgcggcg ggccgcggaa
     3361 agagcttcag caccgccgcc gtgatgatgc caagggtgcc ctctgcaccg atgaagagct
     3421 gcttcaggtc gtagccggtg ttgtccttgc gcagaccgcg caagccattc cagatgccgc
     3481 cgtccggaag cacgacctcg agcccgagca cgagatcacg cgtattgccg taacgcagca
     3541 cgttcacgcc gccggcattg gtcgacagat tgccgccgat ctggcagctt ccctccgcgc
     3601 cgaggctcaa gggaaaaagc cgatcggcgt cggcggcggc ctgctggatg ttcgccaaga
     3661 tgcaccccgc ctcgaccgtc atcgtgttgt tgagcgcgtc cacttccagc acgcggctca
     3721 tgcgcgagag cgtgaggatg atctcgttgc cgctcgcatg cgggacggaa gcgccgcaca
     3781 tgccggtatt gccgccctgc ggtacgatcg gagtccggct ttccgcacag agcttgacga
     3841 ctgcggcgac ttcctcggta ttggccgggc gtacgacagc aggcgttgcg ccgcggaaga
     3901 tgccgcgcca gtctacgacg tgcggctcga catccgccgc gtccgtgagg agaccttgcg
     3961 gcccgacgat ggcggcgatc cgttcgatca gcgtatgcgc aggcatatcg cgcgcattat
     4021 agcgaggcac accgtgtgcc aaagaaacag gccggcatct tttgaatgcc ggcctgcgtg
     4081 gaaaggcg
//
LOCUS       DASURG010000547         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_92718, whole genome shotgun sequence.
ACCESSION   DASURG010000547 DASURG010000000
VERSION     DASURG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92718"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFY80_09845"
     CDS             <1..336
                     /locus_tag="VFY80_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6008256.1"
                     /translation="KGGIISFTKTLAAQYVDHHIRANAIAPGSVRTERQIRRYENREW
                     AESEKTGAAARERMIAQKLYPYSVSEPSDIAAIALFLASDDSRTLTGTTIAADGGRSS
                     YLRFNTGEP"
     gene            487..1539
                     /locus_tag="VFY80_09850"
     CDS             487..1539
                     /locus_tag="VFY80_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008257.1"
                     /translation="MEIELKSRRHTVEDPDAALELCYAKGWTDGLPVVPPTRGRIAAM
                     LDAAGLEPQRQLAFIENRQVSVTAEKVAINAVMAGCKPDYMPVIAAAVEALGDPLYGY
                     HGPATSTGGSAVFMVVNGPIARALDINYENNLFGPGWRANATIGRAVRLVMRNVIGTL
                     PGELDRSSLGHAGKYTFCIAENEAESPWPPFHTTRGFRADQNVVTIFAAYAPHQYSNR
                     LSATPEGVLDTCCAHMKISAGTARQPQYALVFSGEHSAIMKKAGWSREDVQRYCFEHS
                     QSSYAELKRANLLPGEVTSEDERKMYPLVERPQDFLVISAGGRAGVQSAFIPGWGGKN
                     GSQSVSREIRQRRDDA"
     gene            1798..>2049
                     /locus_tag="VFY80_09855"
     CDS             1798..>2049
                     /locus_tag="VFY80_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008258.1"
                     /translation="MALEIYDPTTEATGRRIDYAARPKSLAGLRIGLVDNTKHNSDQL
                     LLRIAAILEREHGAKSHVIRKKRSAGAAPSPEMIAEYKSN"
BASE COUNT          392 a          661 c          663 g          333 t
ORIGIN      
        1 aagggcggca tcatctcgtt cacgaagacc ctcgcggcgc agtatgtcga tcaccacatt
       61 cgcgccaacg ccatcgcgcc gggatcggtg cggaccgagc gtcagatcag gcgctacgag
      121 aaccgggaat gggccgaatc ggagaagacg ggcgcggctg cgcgcgagcg catgatcgcc
      181 cagaagctct atccctattc ggtgagcgag ccgtccgata tcgcggcgat cgcgcttttt
      241 ctcgcgtccg acgattcgcg cacactcacg ggcaccacca tcgccgccga tggcggacgt
      301 tcctcttacc tccgtttcaa caccggggag ccctgatcgg ccgcggcgcg ggcagcattg
      361 cggcggacct tctcgggttg gcccggcgct cgaggcgggc gcgcggtttg caggcggtgc
      421 cgtgcggtgc ataatgcacc ctcgctgcat ttcccggcgg tacatcaacg aaaagagagg
      481 tcgcggttgg agatcgaact gaagtcgcgg cgccacacgg tggaggatcc cgacgctgcc
      541 ttggagctgt gttacgccaa aggctggacc gacgggctgc cggtcgtgcc gccgacccgc
      601 gggcgcatcg cagccatgct ggacgccgcg gggctcgagc cgcagcgaca gctcgccttc
      661 atcgagaatc gccaggtctc cgtcaccgcg gagaaagtcg cgatcaatgc ggtgatggca
      721 ggttgcaagc ccgactacat gcccgtaatc gccgcggcgg tggaagcgct cggcgatccg
      781 ctctacgggt accacggtcc ggcgacgagc accggcggct ccgcggtgtt catggtcgtg
      841 aacggcccca tcgcccgagc gctcgacatc aactacgaaa acaacctgtt cgggccgggg
      901 tggcgcgcga acgcgaccat cggacgcgcc gtgcggctcg tcatgcgcaa cgtcatcggc
      961 acgttgcccg gcgagctgga ccgcagcagc ctgggccatg ccggcaagta cactttctgc
     1021 atcgccgaga acgaagcgga gagcccgtgg ccgccgtttc acacgacgcg cggctttcgc
     1081 gccgaccaga acgtcgtcac gattttcgcc gcctatgcgc cccatcagta ttcgaaccgc
     1141 ctgagcgcga cgcctgaggg cgtcctcgat acgtgttgcg cgcacatgaa gatctccgcg
     1201 gggacggcgc gccagccgca gtacgcgctg gtattctccg gggagcattc cgccatcatg
     1261 aagaaggccg gctggtcgcg cgaggacgtg cagcgttact gcttcgagca ctcgcaatcg
     1321 agctacgcgg aactcaagcg cgcgaatctt ctgcccggcg aggtaacttc cgaggacgag
     1381 cgcaagatgt atccgctggt cgagcgtccg caggattttc tcgttatttc cgcgggcggg
     1441 cgtgcgggcg tgcagtccgc gttcattccg ggctggggcg gcaagaacgg ctcgcagagc
     1501 gtgagccgcg aaatccggca gcgccgcgat gatgcgtaac tcgctttccg gcgaaaggtc
     1561 tttctcgttt tcccggcgtc gcgagcctgc cccggaaggg tgtgtaagcc cggagccggg
     1621 atccagaaca cccgcctgga ttccggtttt caccggaatg acgaaatagc ggaatgacga
     1681 aagcactttc acctacgaaa gcatttgcgc cgaatgacga aagcgctttc ccaggaatga
     1741 cgtgagtatc gtcatcccgg cgaaagccgg gatcaggatt cacagagagg agaaaccatg
     1801 gctcttgaaa tttacgaccc gaccactgaa gcgaccggcc ggcgcatcga ctatgcggcg
     1861 cgcccgaaat cactcgcggg gctacgcatc gggctggtgg acaacaccaa gcacaactcc
     1921 gaccagctgc tgctccgcat cgcggccatc ctcgagcgcg agcacggtgc gaaatcgcac
     1981 gtcatccgca agaagcgcag cgccggggcg gcaccatcgc cggaaatgat cgcggagtac
     2041 aagtcaaac
//
LOCUS       DASURG010000548         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1319316, whole genome shotgun sequence.
ACCESSION   DASURG010000548 DASURG010000000
VERSION     DASURG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319316"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFY80_09860"
     CDS             <1..576
                     /locus_tag="VFY80_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008259.1"
                     /translation="QVTVSREQEPRTIREAVCVFNRPEDLQDAIDELLSSGFHRAELS
                     LLASEHAVEEKLGHRYEKVNALADHPTVPRAAYVSTEAIGDAEGGLIGGLMYVGAVAG
                     AGAIVASGGTLAAIIAATAVAGGAGGLVGSILAKWLGNHHAHYLQEQLDQGGLLLWVR
                     TRTAEDERRAITVLRKHSGREVHVHALPSEA"
     gene            1037..1894
                     /locus_tag="VFY80_09865"
     CDS             1037..1894
                     /locus_tag="VFY80_09865"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX6008260.1"
                     /translation="MTKTDALIITSSDQVPRRTALKRFPIPRNFGVSPGSRIERGRTW
                     PAPAGASRVKAFEIYRYDPDSGDNPRLDTFEVDLDDCGPMVLDALFWIKNNIDSTLTF
                     RRSCREGICGSCAMNMDGTNWLACTRYIEETDAPARIYPLANMRIVKDLVPDLSHVFA
                     QYEMIEPWLQAKTPAPDRERLQAPEDRDKLNGYYECILCFCCTSGCPSHWWNGDRFLG
                     PAVLLQAWRWLTDSRDEATGERLDKLEDPFRLYRCHTILNCTRTCPKGLNPGKAIAEI
                     KKMIADRRG"
     gene            1905..2312
                     /gene="sdhC"
                     /locus_tag="VFY80_09870"
     CDS             1905..2312
                     /gene="sdhC"
                     /locus_tag="VFY80_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX6008261.1"
                     /translation="MERIPSRDRRPLSPNIQIYRPQLTSLLSIVHRITGVFLSACAVV
                     LGIWLIAAARGPEAYSRFHDLIGSWPGQIFLLAATFAFFLHLCGGIRHLVWDTVRGFE
                     LSQIYASGWLVVAASVVLTLAAWLASLLIAGQS"
     gene            2312..2698
                     /gene="sdhD"
                     /locus_tag="VFY80_09875"
     CDS             2312..2698
                     /gene="sdhD"
                     /locus_tag="VFY80_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX6008262.1"
                     /translation="MERYRFRSPLGRAVGLGSAKAGFGHWWTERVTAVALVPLSVWFA
                     ASLIAHSGSGYETFTGWLSSPVTAVLMILLLIALFWHAALGLQVVIEDYVHSGVKVWA
                     LLATRFLCFALAVAGILATLRVAVGV"
     gene            2835..3164
                     /locus_tag="VFY80_09880"
     CDS             2835..3164
                     /locus_tag="VFY80_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6008263.1"
                     /translation="MALHHAKPGEVVDLRPLGNRLKDEKTEAIIKAARFEAIRLVVMA
                     GNEIPSHEVSGNITLHCLEGRISLGLAQSSIELNAGEWVYLDGGEPHSVKGIEDSSLL
                     LTILFDK"
     gene            3867..>4210
                     /locus_tag="VFY80_09885"
     CDS             3867..>4210
                     /locus_tag="VFY80_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6008264.1"
                     /translation="MIVSTSALYGQGYYKGLDMLTLLKAQKIVNRALRRARELNINIS
                     VAVCDPNGRLIALNQMDGSVGWEEDRCSMGKAVAAAITGRPSDRLLEQLDQGVLRLSS
                     MHNVVPPRGQRGG"
BASE COUNT          812 a         1194 c         1310 g          894 t
ORIGIN      
        1 caagtcacag tgtcgcgcga acaggagcct cgaactatcc gcgaagcggt ttgcgttttc
       61 aaccgccccg aggacctgca agatgcaatc gacgaactgc tcagttctgg cttccatcgt
      121 gccgagttga gcctactcgc cagcgagcac gctgtggagg aaaagctcgg ccatcggtat
      181 gagaaggtca acgctctggc cgaccatccg acggtgccgc gagcagctta cgtgtcgacc
      241 gaggctatcg gagatgccga gggcgggctg atcggtgggt tgatgtatgt cggagcggtg
      301 gcaggggccg gtgcgatcgt cgcatctggc ggaacgcttg cggcaattat cgctgcaacg
      361 gcggtggcgg gcggggccgg aggtttggtc ggctcgattc tggccaaatg gctggggaat
      421 caccatgcgc attatctaca agagcagctc gatcagggcg gcttgctgtt gtgggtgcgg
      481 acacggactg ccgaggacga aaggcgcgcc atcacagttc tcagaaagca ttcgggccgc
      541 gaagttcacg ttcatgcctt gccgtcagag gcgtaaaacg cgacaatcga cgtgcgcccc
      601 ttatcggccg tacccggcag tagacgtcgt tcgtatgtcg ctttgacgtg tttgtcggac
      661 gacaggtaaa tttgaacaga tcgcggcggg aaaagtaacc ctcaagctgc cgattgcgtt
      721 cgcgctcgac ttcgcgtctc tcaattccaa cgtttgcttt ttctcggcat accagacctc
      781 gatttgtttc agccgatccg aattccgcat gtcctgcagc gggtgtctca ccgaaaagca
      841 ggaaatccgg gcagccgttt tgctttccgg ccgcaaggat aagacggttg cgcgtccccg
      901 caaccaacct ccttccatta tcgtcatggc cgcgtacggc catccgcgtc tttcccttat
      961 tgtctaaaga agcacgtgga taggaaccgc ccacggagga cgccgtttgc gagttcatat
     1021 aatactggaa atagaaatga cgaaaaccga cgcgctcatc atcacctcgt ccgatcaggt
     1081 tccccggcgc accgcgctta agcggttccc gatcccccgc aatttcggcg tgtcgcccgg
     1141 ttcgcgcatc gagcgtgggc gaacatggcc cgcgccggcg ggcgcgagtc gcgtgaaggc
     1201 gttcgagata taccgctacg atccggacag cggcgataac ccccgcctcg acacattcga
     1261 ggtcgatctt gacgattgcg gccccatggt cctcgacgcg ctcttctgga tcaagaacaa
     1321 catcgattcg acgctgacct tccggcgctc ctgccgggag ggcatctgcg gttcatgcgc
     1381 catgaacatg gacggcacga actggctggc ctgcacgaga tacattgagg aaacggatgc
     1441 gccggcaaga atctatccgc tggcgaacat gaggatcgtc aaggacctcg ttcccgatct
     1501 cagccatgtc ttcgcgcaat acgagatgat cgagccctgg ctccaggcga agacgcccgc
     1561 gccggaccgg gaacggctgc aagcgccgga ggaccgcgac aagctcaatg gctactacga
     1621 gtgcatcctt tgcttctgct gcacctccgg ctgcccgagt cactggtgga acggggaccg
     1681 tttcctgggt cccgccgtgc ttttgcaggc ttggcgctgg ctgaccgaca gccgcgacga
     1741 ggcgactggc gagcggctgg acaagctgga ggacccgttc cggctctatc ggtgccatac
     1801 cattctcaac tgcacccgga cctgtccaaa ggggctcaat ccgggcaagg ccatcgcgga
     1861 gatcaagaaa atgatcgccg accggcgggg ctgaggagat gggtatggaa cgtatcccaa
     1921 gtagggatcg ccggccgctt tcgccaaaca tccagatata caggccgcag cttacttccc
     1981 ttctgtccat cgtccaccgg attaccggcg tcttcttgag cgcctgcgcc gttgtactcg
     2041 gcatctggct gatcgcggcg gcgagagggc cggaggctta ttcccggttc catgatctaa
     2101 tcggatcgtg gcccggacag atttttctgt tggccgccac attcgcgttt tttttgcatc
     2161 tttgcggcgg tatccgccat cttgtttggg acacggtgcg cggctttgag ctgagccaga
     2221 tatacgcctc ggggtggctc gtcgtcgccg cgagcgtggt gctgacgctg gctgcttggc
     2281 tcgccagcct gctgatcgcg gggcagagct gatggagagg tatcgctttc gctccccgct
     2341 cggccgggcc gtgggcctcg gatccgccaa ggcggggttc ggacactggt ggacggagcg
     2401 ggtgacggcg gtggcgctcg tgcccttgag cgtatggttc gccgcctcgc tgatcgccca
     2461 tagcggcagt ggctacgaga cattcacggg ctggctgagc tcgcccgtga cagcggtcct
     2521 gatgatcttg ctgctgattg cgctcttctg gcacgcggcg cttggtctcc aggtggtgat
     2581 cgaggattac gttcattccg gtgtaaaggt ctgggcactg ctggcgacgc gcttcttgtg
     2641 cttcgcgctg gcggtggccg gcatcttggc gacgctgcgg gttgccgtcg gcgtgtaagc
     2701 ggccatctga gtgcaagaag ggctgggggg aggaacgaga atcgtaggga aggaagggag
     2761 cgtgggatat cgcggatgac ccgcgcgttc gatgagccgc gaaatgcaaa acaaatgaaa
     2821 ggaacattgc aataatggca ttacatcatg cgaaaccggg agaagtcgtt gacctgcgac
     2881 cgctggggaa caggctaaag gatgaaaaaa ctgaagcgat catcaaagcg gcgaggttcg
     2941 aggcgatccg tcttgtcgtt atggccggca acgaaattcc ttctcacgaa gtgtcgggca
     3001 atatcacgtt gcattgcctt gaaggacgca tatcgctggg cttggcgcaa tcctccatcg
     3061 agcttaacgc tggcgaatgg gtgtatcttg atggtggtga acctcattcc gtgaaaggca
     3121 tcgaggattc gtcccttctt ctcacgatcc tcttcgacaa gtagcgtgag gagtcccata
     3181 aacagcatct tggttggtct tcttgttttt taggactacg cgcctcggcg acatcgagca
     3241 ccggttccag tgcggaatcc agaagcagct tcaccaagtc gggccattgg ccgcgggagt
     3301 cattgatgcc ttgtttgttc tcctcgtagc gggcgagacg gtgattgccg ggcgcaactg
     3361 gcgcaaccag cgccgatttt cattctcctg atcttcctcg tcgacaacgt gagtttccat
     3421 attgaagatt agagcagtgg aacacgccga aggcggaagc cgtctcggca ttcccgccgc
     3481 tgtcgcactg acctcgccga tcgccggctg tatggtgccg cgcttcatgc caaactggct
     3541 ggccggggag aaccgggctg gctcccgggg ccgttcggcc agtttgactt ggtgacattg
     3601 gctatgcccg tcattgcact ggcatcttgg gtggcaagtc cagcgtggct gggcacagct
     3661 gcattgatgt tgttggcggc ggcggcgcaa gtgaggcctc tggcccattg ggcgggcgac
     3721 cgaacgatgc gcgaccggct gatcgccagc cttcacatcg cctaatgtcc ttaagtcgga
     3781 ggcccaggga gcattatatt ttttgactaa ttatatgcgt ttgtccgcag tcgtcgcaga
     3841 caccatatta caattgaagc caaggaatga tcgtatcgac ttcggcatta tacgggcagg
     3901 gctactacaa aggcttggat atgctcaccc ttttgaaagc gcaaaaaatc gtcaaccgag
     3961 cgctgcgacg ggccagggag cttaacatca acatcagcgt agcggtctgc gaccccaatg
     4021 gccgactcat cgccctgaat caaatggatg gttctgttgg ttgggaggag gaccgctgct
     4081 cgatgggaaa ggcggtcgct gctgccatta ccgggcgccc gagcgatcgg ctcttagagc
     4141 agctcgatca aggtgtcctg aggctatcct caatgcacaa tgtagtccct ccgcggggcc
     4201 agcgtggcgg
//
LOCUS       DASURG010000549         6947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1216886, whole genome shotgun sequence.
ACCESSION   DASURG010000549 DASURG010000000
VERSION     DASURG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216886"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFY80_09890"
     CDS             <1..554
                     /locus_tag="VFY80_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HEX6008265.1"
                     /translation="ADAAWTKNEMERASSAFLRLYDAPAHAHAATEWRMNRHAWRLIQ
                     RFGFRYASDTRGHCPFIPVCNAEIIACAQLPTTLPTLDEMMAQDARTPDDCVPKLLEA
                     TARAIATPHVFTLRAEREGGKLFPALQTLLDGWHKQGYLFCALEDCLADLNLARLPRH
                     SVSEVSLPGRLRPVSMQGEEFLA"
     gene            629..1087
                     /locus_tag="VFY80_09895"
     CDS             629..1087
                     /locus_tag="VFY80_09895"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6008266.1"
                     /translation="MVDQPAPDFALPATGGKTFRLSDAKGSTLVLYFYPKDNTPGCTR
                     EGEQFRDLHPDFRELRCEIYGVSRDSLKAHESFKSKMKFPFDLVSDTEETACRLFDVI
                     KLKNMYGRKVRGIERSTFVIDARGIVRRAWRGVKVPDHAREVLEFVKTLK"
     gene            1128..2555
                     /locus_tag="VFY80_09900"
     CDS             1128..2555
                     /locus_tag="VFY80_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6008267.1"
                     /translation="MTPRKSMSVVARFPATTKLFVLDTNVLMHDPTSLFRFEEHDLYI
                     PMATLEELDNNKKGLSEVARNARQASRYLDELISHAGPNIAEGIALEAHGDKSATGRL
                     FLQTEAIESDLPVRLASGKADNQIIGVVKHLQEHHPKRDVILVSKDINMRIKARALGM
                     PAEDYFNDKALEDTDLLYTGTRALPQDFWDKHGRDMESWQQAGHTYYRVRGPICSSLL
                     VNQFVYQEGEDKPFYAQVKEVSGKTAILQTLKDYAHQRHNVWGITARNREQNFALNVL
                     MNPEIDFVTLLGQAGTGKTLLTLAAGLVQTLESKTYSEIIMTRVTVPVGEDIGFLPGT
                     EEEKMNPWMGALEDNLDVLNKTDDEAGEWGRAATRDLIRSRIKVKSLNFMRGRTFINK
                     YLIIDEAQNLTPKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSGITYVVDRMKG
                     WAHSGHVTLARGERSRLADYAAEAL"
     gene            complement(2640..4040)
                     /gene="dnaB"
                     /locus_tag="VFY80_09905"
     CDS             complement(2640..4040)
                     /gene="dnaB"
                     /locus_tag="VFY80_09905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX6008268.1"
                     /translation="MQVVTPIARESLVEAARVPPQSIEAEQSVLGGLLLDNSAWDRVS
                     DVITEESFYRADHRTIFHHIVQLIENNRPADALTVAESLERSAKLEDIGGRAYLSALS
                     VNVPSAANIRRYAEIVRERAIMRDLAAVGTDIAESAFNPMGKDAAVLIDEAEARVFQI
                     AEAGTRSRQGFTKIDPLLTETVERIDMLYSRENKDDIIGLATGFIDLDRMTSGLHGGD
                     LIIIAARPSMGKTTLAMNIVEHVALNLRKAAAVFSMEMSGAQLTMRMIGSVGRVDQHK
                     LRTGTFSEEDWPKLVEAVGKLNEAHIYIDDTAGLNALEVRSRARRLHRQCAETGGLSL
                     IVVDYLQLMSASRGGREENRATEIAEISRSLKGLAKELKVPVVALSQLNRGVESRQDK
                     RPMMSDLRESGAIEQDADLIFFIYREEVYNPDTPNKGVAEIILAKQRNGPTGMKKLTF
                     LGAHTRFENFADTNRF"
     gene            complement(4328..4777)
                     /gene="rplI"
                     /locus_tag="VFY80_09910"
     CDS             complement(4328..4777)
                     /gene="rplI"
                     /locus_tag="VFY80_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6008269.1"
                     /translation="MQVILLEKVANLGQLGDVVKVRDGFARNYLIPHGKARRATPENL
                     AEFEQRRADLEKAQAEALAGAQEKAAQLEGLTLQIAQKAGVDGKLFGSVTNFDIAEAL
                     AKRGVEVSKAAIRMPQGPLKAVGDHPLKVALHTDVVVTITVSVVAEQ"
     gene            complement(4790..5086)
                     /gene="rpsR"
                     /locus_tag="VFY80_09915"
     CDS             complement(4790..5086)
                     /gene="rpsR"
                     /locus_tag="VFY80_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6008270.1"
                     /translation="MARLGPSRGKPKGKGKGKGERRGGRGQLFRRRKFCRFTAEGIKE
                     VDYKDINLLKDFINENGKIIPARITGTKARYQRQLSVAVKRARFLALLPYTDLH"
     gene            complement(5046..5372)
                     /gene="priB"
                     /locus_tag="VFY80_09920"
     CDS             complement(5046..5372)
                     /gene="priB"
                     /locus_tag="VFY80_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEX6008271.1"
                     /translation="MSGELMEIEPLRHTPAGIPLLNFRLAHRSQQMEAGFRRQVECEL
                     NGIAIGEAAVAMARMATGATVRVEGFLNRKNRMSVQLVLHVTQVRTLEDSRHGETRTE
                     QGQAQG"
     gene            complement(5393..5791)
                     /gene="rpsF"
                     /locus_tag="VFY80_09925"
     CDS             complement(5393..5791)
                     /gene="rpsF"
                     /locus_tag="VFY80_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6008272.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYRQMITNRGGRIHRLEDWGR
                     RQLAYLIEKVHKAHYVLMNIECDNETLTELEHAFKFNDAVLRHLTVKMSAPVTTPSPM
                     MKDEKSKSLATGSEEAPKAPAAPEQAPAAA"
     gene            complement(5920..6675)
                     /gene="rlmB"
                     /locus_tag="VFY80_09930"
     CDS             complement(5920..6675)
                     /gene="rlmB"
                     /locus_tag="VFY80_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX6008273.1"
                     /translation="MPDTRLIFGFHAVASRLRQNAGSVREIYFDNARHDRRAQDFLNL
                     AEQRGVRVMRVDAKRLDGMTSNARHQGVAARVEAVRLPVHIDEVLNNLHEPALLLVLD
                     GVTDPHNLGACLRVADAMGVHAVVAPKDRAVGINATVAKVASGAADTVPYVAVTNLAR
                     TLRDLKERGVWLYGADNSAERELFGTRLDGALAWVLGAEGEGLRRLTRETCDEIVRIP
                     MYGSVESLNVSVSAGICLAETRRQRLAPNVATR"
BASE COUNT         1277 a         2261 c         2090 g         1319 t
ORIGIN      
        1 aagcggatgc cgcctggacg aagaacgaaa tggagcgggc ctcttctgcg ttcctccggc
       61 tttacgatgc gccggcgcat gcgcatgctg ctaccgagtg gcgcatgaac cgccacgcat
      121 ggcgcctcat ccagcgcttc ggtttccgct acgcgagcga cacacgcggg cactgtccgt
      181 tcatcccggt gtgcaatgcc gagatcatcg cgtgcgcgca gctgcccact acgctgccca
      241 cgctcgatga aatgatggcg caggacgcgc gcacgccgga cgattgtgtt ccgaagctcc
      301 tcgaagcgac cgcgcgcgcc attgcgacgc ctcacgtctt cacgctgcgc gcggagcgcg
      361 aaggcggcaa gctctttcct gcactgcaaa cactcctcga tggctggcac aaacaaggct
      421 acctgttttg cgcactggaa gactgcctcg ccgatctcaa ccttgcccgc cttccgcgcc
      481 acagcgtgag tgaagtcagc cttccgggaa gactacgccc cgtcagcatg caaggcgaag
      541 aattcctcgc gtaacttgcg cgccgcgcgc gattcgttca acattcacaa caaagcgata
      601 cggccggaag gacaagcagc cgccccgaat ggtagaccag cctgcgccag atttcgcgct
      661 tcccgcgacc ggcggcaaga ccttccgcct ctcagacgcc aagggcagta cgctcgtcct
      721 ctacttctac cccaaagaca acacgccggg atgcacccgt gagggcgagc agttccgcga
      781 cctgcatccc gacttccgcg agctgcgctg cgagatctac ggcgtttcgc gggacagcct
      841 gaaggcgcac gaaagtttca agtcgaagat gaagtttccg ttcgatctcg tctcggacac
      901 cgaggagacg gcgtgccggc tcttcgacgt catcaagctc aagaacatgt acggcaggaa
      961 ggtgcgcggc atcgagcgca gcacctttgt gatcgatgcc cggggcatcg tgcgtcgcgc
     1021 gtggcgcggc gtgaaggtgc ccgaccacgc acgggaggtg ctcgaattcg tcaaaacgct
     1081 gaaataaacg cttgcccgat ccacccgcct gccgcacaag gagaaccatg accccgcgca
     1141 agtccatgtc cgtcgtcgcc cgctttcccg ccaccaccaa gctcttcgtg ctggacacca
     1201 acgtgctgat gcacgacccc acgagcctct tccgcttcga ggagcacgac ctctacatcc
     1261 cgatggcaac cctcgaagag ctcgacaaca acaagaaggg actgtccgag gtcgcgcgca
     1321 atgcgcgcca ggcgagccgc tatctcgatg aactcatcag ccatgccggt ccgaacatcg
     1381 ccgaaggcat cgcgctcgag gcccacggcg acaagagcgc gaccgggcgg ctgttcctcc
     1441 agaccgaggc gatcgagagc gatctgccgg tgcggcttgc gagcggcaag gcggacaacc
     1501 agatcatcgg cgtggtgaag catctgcagg agcaccatcc caaacgcgac gtgatcctgg
     1561 tgagcaagga catcaacatg cgcatcaagg cgcgcgcgct ggggatgccg gccgaggatt
     1621 acttcaacga caaggcgctc gaggacacgg acctcctcta taccggcacc cgggcactgc
     1681 cccaggactt ctgggacaag cacggtcgcg acatggagtc gtggcaacag gccggccaca
     1741 cctactatcg ggtgcgcggg ccgatctgct cgagcctgct cgtcaaccag ttcgtctacc
     1801 aggagggtga ggacaaaccg ttctacgcgc aggtaaagga ggtgagcggc aagactgcca
     1861 tcctgcaaac gctcaaggac tacgcgcacc agcggcacaa cgtgtggggt atcaccgccc
     1921 gcaaccggga gcagaatttt gcgctcaacg tcctcatgaa cccggagatc gacttcgtta
     1981 ccctcctcgg gcaggcggga acgggaaaga cgctgctgac gctcgcggca gggctcgtgc
     2041 agacgctgga aagcaagacg tactcggaga tcatcatgac gcgggtcacg gtgcccgtcg
     2101 gggaggatat tggctttctc cccggcaccg aagaagagaa gatgaaccct tggatgggcg
     2161 cgcttgagga caacctcgat gtgctcaaca agaccgacga tgaggccggc gaatggggtc
     2221 gagccgccac acgcgacctc atccgctccc gcatcaaggt gaagtcgctc aacttcatgc
     2281 gcgggcgcac cttcatcaac aagtacctca tcatcgatga agcgcagaac ctgacgccga
     2341 agcagatgaa gacgctcatc acccgcgcgg gtcccggcac gaaggtcgtg tgcttgggca
     2401 acatcgcgca gatcgacacc ccttacctca ccgaagggag ctccggcatc acctatgtcg
     2461 tcgatcggat gaagggctgg gcgcacagcg gccacgtgac gctcgcacgc ggcgagcgct
     2521 cgcggcttgc agattacgcg gcggaagcgc tgtagtccga gctgttcgcc cgcggcttgc
     2581 ggaagcgcga acgttgggcc gtgcgttcgt gttcccgcgc agtcaatacg acaagggcgc
     2641 tagaaccggt tcgtatcggc gaaattctcg aagcgcgtgt gcgcgccgag gaaggtgagt
     2701 ttcttcatcc cggtcggtcc gttccgctgc ttcgccagaa tgatctcagc cacccccttg
     2761 ttgggcgtat cggggttgta gacttcctcg cggtagatga agaagatgag gtccgcgtcc
     2821 tgctcgatcg cgcccgactc ccgtagatcg gacatcatcg gccgcttgtc ctggcgcgac
     2881 tcaacgccgc gattcaactg cgagagcgcg acgacgggga ccttcagttc cttggcgagg
     2941 cccttgagcg agcgcgaaat ctcagcgatc tcggtcgcgc ggttttcctc ccgccccccg
     3001 cggctcgccg acatgagctg cagatagtcg acgacgatca ggctcaaccc tccggtttcg
     3061 gcgcactgcc ggtgcagccg gcgcgcacgc gagcgcactt cgagcgcgtt cagccccgcc
     3121 gtatcgtcaa tatagatatg cgcctcgttg agcttgccca ccgcctccac gagcttgggc
     3181 cagtcttcct cggagaatgt gcccgtgcga agcttgtgct ggtctacgcg accgaccgag
     3241 ccgatcatgc gcatggtgag ctgcgcgccc gacatttcca tgctgaacac cgccgcggct
     3301 ttcctgagat tgagcgcgac gtgctccacg atgttcatcg cgagcgtcgt ctttcccatc
     3361 gaaggccggg cggcgatgat gatcaagtcc ccgccgtgga gccccgaagt catcctgtcg
     3421 agatcgatga agccggtggc aaggccgatg atgtcgtcct tgttttcccg gctgtagagc
     3481 atgtcgatgc gctcgaccgt ctcggtaagc agcggatcga tctttgtgaa gccctgccgt
     3541 gaccgtgtgc ccgcttctgc aatctgaaag acacgcgcct ccgcttcgtc gatgagcacg
     3601 gctgcgtcct tgcccatggg attgaaggcg gactccgcga tgtccgtgcc gaccgcggcg
     3661 agatcccgca tgatggcgcg ctcgcgcaca atctcggcat aacggcggat gttcgccgcg
     3721 ctcgggacgt tgactgaaag tgcgctcaag taggcgcgcc cgccgatgtc ctcaagcttg
     3781 gcgctgcgct cgaggctttc cgcgaccgtc agggcgtccg ctggccggtt gttttcgatc
     3841 agctggacga tatggtggaa gatcgtgcgg tggtccgccc ggtagaagct ctcctcggtg
     3901 atgacgtcgc tgacgcgatc ccaggcgctg ttgtcaagaa gaagcccgcc cagtacggat
     3961 tgttcggctt cgatggattg cggcggtacg cgcgcggcct cgaccagcga ttccctggcg
     4021 atcggcgtga cgacttgcat catgtcccgt ccctgtcgtt cttataggat tttcactgcg
     4081 attgtcatgc accgcgcatc gtcgcggagt gcatggactg cgcgatcaat gtagcgaaaa
     4141 aaaagggccg tcgccagccc tttttctgtg aagcgaaatt ctgttgtgaa atcgcaacga
     4201 gaaatttttt cgggcgcgcg cgcgaacgat gaagtgacgc ttcatgctcg ggcgatgtaa
     4261 agccgacaag catttagcat gccgacttcc gccgagcgag cgcggcaacc gaaactgtcg
     4321 aacgcgctta ctgctcggcg acgactgaaa cggtgatcgt caccacgaca tcggtgtgca
     4381 gcgccacctt gagcggatga tcacccacgg ccttgagcgg gccctgcggc atgcggatcg
     4441 ctgccttcga gacttccacg ccgcgcttgg cgagtgcttc ggcaatgtcg aaattcgtga
     4501 ccgagccgaa aagcttcccg tcgacgccgg ccttctgggc gatctgcagc gtcaggccct
     4561 cgagctgtgc ggccttttcc tgcgcacccg ccagcgcttc ggcctgcgcc ttctcgagat
     4621 ccgcgcgccg ctgctcgaac tccgcgaggt tctcgggcgt cgcacgtcga gccttgccgt
     4681 gcgggatcag gtaattcctc gcgaagccgt ccctgacctt cacgacatcc ccgagctggc
     4741 ccagattcgc gactttttcc agcagaatga cttgcatgag aactcccgct cagtggaggt
     4801 cggtgtaggg cagcagcgcg aggaaccgcg cgcgcttcac cgcaaccgac aattgccgct
     4861 ggtagcgcgc cttcgtgccg gtgatgcgcg ccggaatgat cttgccgttc tcgttgatga
     4921 agtccttcag gaggttgatg tctttgtaat ccacctcctt gatgccttct gcggtgaagc
     4981 ggcagaactt gcgacgccgg aaaagctgcc cgcgcccgcc tctgcgctca cccttaccct
     5041 tgcccttacc cttgggcttg cccctgctcg gtcctagtct cgccatgtcg gctatcctct
     5101 aacgttcgta cctgtgtcac gtgcaggacg agttgcacgc tcatcctgtt cttccgattc
     5161 agaaacccct ctacgcgcac cgtcgcgccg gttgccatgc gagccatcgc cacggccgcc
     5221 tctccgattg cgataccgtt gagttcgcac tccacctgcc ggcgaaagcc cgcttccatc
     5281 tgctgcgatc tgtgcgccag ccggaaattg agcagcggga tgccggcggg cgtatggcgc
     5341 agtggttcga tctccatcag ctcgccgctc atggcgatcg tgttgtgctc cactatgcgg
     5401 cagccggcgc ctgctcaggc gccgcgggcg ccttgggcgc ttcttcgctc cccgtcgcga
     5461 gcgacttcga cttctcgtcc ttcatcatcg gtgaaggggt cgtgaccggg gcgctcattt
     5521 tcaccgtgag atgacgcaga accgcgtcgt tgaacttgaa ggcgtgctcg agctcggtca
     5581 gcgtctcgtt gtcgcactcg atgttcatga gcacgtagtg cgccttgtgc accttctcga
     5641 tcaagtatgc gagttgccgg cggccccagt cctcgagccg gtggatcctg ccgccccggt
     5701 tcgtgatcat ctgccggtag cgctcgacca tggcgggcac ctgctcgctc tggtccggat
     5761 gtacgataaa aacgatttcg taatgtcgca tcgaatgggc tccttgtggg ttgaagcctc
     5821 ccgccatgcg aggagcggtg aggcaaggat gtcggctcaa ggcatgggtg ccgaaccgaa
     5881 ggcgcgcatt ataccgatct cgcgttcctt ttcaaagcat taacgggtcg cgacgttggg
     5941 cgcaagccgc tgacgccggg tttcggccag acagatcccg gccgacaccg acacgttcag
     6001 gctttccacg ctgccataca tcgggatgcg cacgatttcg tcgcaggttt cgcgcgtaag
     6061 ccggcgtagc ccttcaccct ccgcccccag gacccatgcg agcgcgccgt cgagccgggt
     6121 cccaaagagt tcgcgctcag ccgagttgtc ggcgccatag agccagaccc cgcgctcctt
     6181 cagatccctc aaggttcgcg cgaggttggt caccgcgacg taagggacgg tatcggctgc
     6241 gccgctcgca acctttgcga cagtcgcatt gatgcccacc gcccggtcct tgggcgccac
     6301 gacggcatgc acgcccatgg cgtcagcgac gcgcaggcag gcgccgagat tatgagggtc
     6361 cgtcacgccg tccagcacca gcagcagcgc cggctcgtgg agattgttga gcacttcgtc
     6421 gatatgcacc ggcaggcgca cggcttccac gcgcgcagcg acgccctggt ggcgggcatt
     6481 actcgtcatg ccgtcgaggc gtttggcatc gactcgcatc acccgaacgc cgcgctgttc
     6541 tgccagattc aggaaatcct gcgcgcgacg gtcgtgccgc gcgttatcga agtaaatttc
     6601 ccttactgag cccgcgttct ggcgcaggcg gcttgcgacc gcgtgaaagc cgaaaatgag
     6661 gcgggtgtca ggcattcgaa caagagcagc cctgtgtgtc ttccgacttt cctggggtca
     6721 cggctaccct ttgcccgtcc ttcgactccg catgcgctcc gctcagtgcg aacaggctga
     6781 aacagcgctc ggcacggcca ttcctcaatc gagaacgaag tcgatcttcg tcgtctcgag
     6841 atcgacccgc accactttta cgcgcacccg gtcgcccagc cgatagcgtc gtttagtgcg
     6901 ctcgcccatg aggtggtgct tcccggcgtc gaaatgataa taatcga
//
LOCUS       DASURG010000550         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_706456, whole genome shotgun sequence.
ACCESSION   DASURG010000550 DASURG010000000
VERSION     DASURG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706456"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..825)
                     /locus_tag="VFY80_09935"
     CDS             complement(112..825)
                     /locus_tag="VFY80_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6008274.1"
                     /translation="MPAPVVHIENARFRWQRGDAPCLDIAGFTIASGERVFLHGASGS
                     GKSTLLALIGGVLTPEAGHVKVLDADLTKMTGPQRDRFRAHHIGFIFQQFNLVPYLTV
                     IDNVLLACRFSKRRRERALARGATLHDEAVRLLDHLDIDAPLRARPVVQLSVGQQQRV
                     AAARALIGRPELVIADEPTSSLDADRQAAFLDLLVRECSEAGVTLIFVSHDMRLAANF
                     TRVIELASFNRPAAEREAA"
     gene            complement(835..1386)
                     /locus_tag="VFY80_09940"
     CDS             complement(835..1386)
                     /locus_tag="VFY80_09940"
                     /inference="COORDINATES: protein motif:HMM:NF022439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HEX6008275.1"
                     /translation="MKFRQSLVVAALANISSYVWAQGHTHVHGIATLQVAVEGAALTL
                     RLVTPLDNLVGFEHAPRTEQQKKALREAEEALRAATVLFVPSAGARCAASSVKVDSPF
                     AAKSDAKDRGEKAKSGKEVAEAHSEFTAEYAFRCEQSERLQGVEVKLFDRFRGVRRLD
                     VELAGPRGQKSLRLTPKQRIIAW"
     gene            complement(1386..>2997)
                     /gene="polA"
                     /locus_tag="VFY80_09945"
     CDS             complement(1386..>2997)
                     /gene="polA"
                     /locus_tag="VFY80_09945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6008276.1"
                     /translation="APDQLALALVLEKLKSWLENRDRAKLGQCIKYDMHVFANHGIKL
                     SGIAHDTLLQSYVLESHKPHDMDNLAERHLNMQTLSYDEVTGKGASRIAFEQVAIDRA
                     TEYAAEDADVTLQLHGCLYPQIERDDKLKRIYAEIEMPVTRVLQVMERNGVRLDTRLL
                     GELSREFGAKIMALEAKAHEIAGQPFNLGSPRQIQDILFEKQQLPVRKKTPSGQPSTD
                     EDVLAELAQDYPLPQIILEHRGLAKLKSTYTDKLAEMINAGTGRVHTNYAQAVAVTGR
                     LASNDPNLQNIPIRTAEGRRIREAFIADECCHIVSADYSQIELRIMAHISQDANLLRA
                     FAEGEDIHRATAAEIFGLQPDEVDAEQRRYAKVINFGLIYGMSAFGLSRQLGIERSAG
                     QQYMERYFARYPGVAQYMQRTREAARAQGYVETVFGRRLWLPEIRSSNAARRGNAERA
                     AINAPMQGTAADLIKLAMIAVQNWIEGETLRTRLIMQVHDELVLEVPDEELSRVRAEL
                     PRLMGSVAQLSVPLLVEVGIGLNWDEAH"
BASE COUNT          507 a          999 c          954 g          537 t
ORIGIN      
        1 ggcgctccac gccgagcagc agcgcgacgc tcaatgcaat ggcaatcacc gtcagcccga
       61 gcgtgaggcg ccggttccag gcgctgcgta cggcgagcgc aaggagatga ctcacgccgc
      121 ctcccgttcg gccgcgggac gattgaagga ggcaagctcg atcacgcgcg tgaagttcgc
      181 cgcaagccgc atatcgtggc tcacgaagat aagcgtgacg cccgcttcgc tgcactcgcg
      241 aacgagcaga tcgagaaacg ccgcctggcg atctgcgtcg agcgaagagg tcggttcgtc
      301 agcgatcacg agctcgggcc gcccgatgag cgcgcgcgct gcagccaccc gctgctgctg
      361 gccgacgctt aactgcacca ccggccgggc gcgcagcggc gcgtcgatgt cgagatgatc
      421 gagcaggcgc accgcctcgt cgtgcagcgt ggcgccacgc gcgagcgcac gctcgcgccg
      481 tcgcttcgaa aaccggcacg cgagaagcac gttgtcgatc acggtaagat atggcacgag
      541 gttgaactgc tggaagataa agccgatgtg atgggcgcgg aagcgatcgc gttgcggccc
      601 ggtcattttc gtgagatcag catccagcac cttcacgtgc ccagcctcgg gcgtgagcac
      661 cccgccgatc agcgcgagca gcgtgctctt gcccgagccg ctcgcgccgt gcaggaaaac
      721 ccgctctccg cttgcgatcg tgaagccggc gatgtcgagg cacggcgcgt cgccgcgctg
      781 ccagcggaag cgcgcattct cgatgtggac gacgggtgca ggcatcgtca gtccctacca
      841 ggcgatgatc cgctgcttcg gcgtcagccg gagcgacttc tgcccgcgcg ggccggcgag
      901 ttccacatcc aggcgccgca cgccgcgaaa gcggtcgaag agcttgacct ccacgccctg
      961 cagccgctcg gactgctcgc agcggaaggc gtactccgca gtaaactcgg aatgcgcctc
     1021 ggccacctcc ttgcccgatt tcgctttttc gccgcgatct ttcgcgtccg atttcgcggc
     1081 aaagggcgaa tcaaccttga cggaagaggc tgcgcaccgc gcaccggcgg agggaacgaa
     1141 gagcaccgtc gccgcacgca aggcttcctc cgcttcgcgc aacgcctttt tctgctgctc
     1201 ggtgcgtggc gcatgctcga atcccaccaa attatcgagc ggcgtcacga ggcgcagcgt
     1261 gagcgccgcg ccctccaccg ccacctgcag agtggcgatt ccgtgcacgt gggtgtgccc
     1321 ctgcgcccag acataggacg agatgttcgc caaggcggcg acgaccagac tctgtcggaa
     1381 cttcatcaat gcgcctcgtc ccagttgagc ccgatgccga cctcgaccag caacggcacg
     1441 gaaagctgcg caaccgaccc catgaggcgc ggcagctccg cccgcacgcg actgagctcc
     1501 tcgtccggca cttcgaggac gagctcatcg tgcacctgca tgatgagccg cgtccgcaat
     1561 gtctcgcctt cgatccagtt ctggacggcg atcatcgcga gcttgatcaa gtcggctgcg
     1621 gttccctgca tcggcgcgtt gatcgccgca cgctcggcgt tgccgcgtcg cgccgcgttg
     1681 gacgaacgga tctccggcag ccagaggcgg cggccgaaaa cggtttcgac gtaaccctgt
     1741 gcgcgtgccg cctcgcgcgt gcgctgcatg tactgcgcga cccccgggta gcgggcgaaa
     1801 taacgctcca tgtactgctg ccccgccgag cgttcgattc caagctggcg cgagagcccg
     1861 aacgccgaca tgccgtaaat caacccgaag ttgatgacct tcgcgtagcg ccgctgctcg
     1921 gcatcgacct cgtcgggttg caggccgaag atctccgccg ccgtcgcgcg gtggatatcc
     1981 tcgccctcgg caaaggccct gagcagattc gcgtcctgcg agatgtgcgc catgatccgc
     2041 agctcgatct gcgagtagtc cgccgacacg atgtgacagc attcgtcggc gatgaaggct
     2101 tcccggatgc ggcggccctc cgcagtgcgg atcgggatgt tctgcaggtt gggatcgttg
     2161 ctcgcgagcc ttcccgtcac ggccactgcc tgcgcatagt tggtatgcac gcggccggtg
     2221 ccggcattga tcatctccgc gagcttgtcg gtgtaagtgg acttgagttt cgcgagcccg
     2281 cggtgctcca gaatgatctg tggcagcgga tagtcctgcg cgagctcggc gagcacgtcc
     2341 tcgtcggttg aaggctgtcc cgaaggcgtt ttcttgcgca cggggagctg ctgcttctcg
     2401 aagagaatgt cctggatctg cctgggcgag ccgagattga acggctggcc cgcgatttcg
     2461 tgcgccttcg cctcgagcgc catgatcttc gcaccgaact cgcgagacag ctcgcccagt
     2521 agccgcgtgt cgagcctgac gccgtttcgc tccatgacct ggagcacgcg cgtgaccggc
     2581 atctcgatct cggcatagat gcgcttgagc ttgtcatcgc gctcgatctg cgggtacaga
     2641 caaccgtgca gctgcagggt cacgtcggcg tcctcggccg catactcagt cgcccgatcg
     2701 atcgccacct gctcgaacgc gatgcggctc gcgcccttgc cggtcacctc gtcgtaactc
     2761 agagtctgca tgttcaagtg ccgctcggcg aggttatcca tgtcgtgcgg cttgtggctt
     2821 tcaagcacgt acgactggag cagcgtgtcg tgcgcgatgc cggagagctt gatgccgtga
     2881 ttggcgaaga cgtgcatgtc gtacttgatg cactgtccca gcttcgctcg gtcccggttc
     2941 tcaagccagg atttcagctt ttcgagcacc aacgcgagcg caagctggtc cggcgcc
//
LOCUS       DASURG010000551         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1114976, whole genome shotgun sequence.
ACCESSION   DASURG010000551 DASURG010000000
VERSION     DASURG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114976"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFY80_09950"
     CDS             <1..622
                     /locus_tag="VFY80_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6008277.1"
                     /translation="NYVSVTTGVRGEGDLEGPEHFHVILLDAGRTRLVGTDLEPMLRC
                     IRCGACMNHCPVYQSVGGHVYGWVYPGPMGSVLTPSYVGLENARDLPNAATFCNQCGV
                     VCPVKIPLPDLMRKLREREFEERLKPWSERIALAAWSWLAQRPALYGWTAGIGARVLA
                     AMGGKSKLIHNLPVGRGWTRGRDFPAPEGRTFRELYASREKGRRPG"
     gene            658..>2056
                     /locus_tag="VFY80_09955"
     CDS             658..>2056
                     /locus_tag="VFY80_09955"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX6008278.1"
                     /translation="MWVNGAKQAAKSTRYGEVTNPATGVVTKRVPFCARSDIRTAVAA
                     AQAAFPKWRDTPPLRRARVLMRYRELLEAHREELARLITEEHGKTLSDAAGSVQRGIE
                     VVEFAMGIPHLVKGEHSEEVGTGVDCHSLRQPLGVCVGITPFNFPVMVPLWLYPVAIA
                     CGNTFVLKPSEKVPSASMRMAELFTEAGLPDGVFNVVHGDKEAVDALLEHPDVKAVSF
                     VGSTPIAKYIYETCAKNGKRVQALGGAKNHAVVLPDADLDFAADALIGAAYGSAGERC
                     MAISAVVAVGDIGEALVGRLRDKAARLKIGPGTDKDVDMGPLVTRAHRDRVAGYVDTG
                     VAEGAKLVLDGRAFEAAGREGFFLGASLFDHVTPDMTIYKDEIFGPVLSVLRAKTLDE
                     SIHLINANPYANGTAVFTRSGAAARKFEREIEVGMVGINVPIPVPIAFYSFGGWRSSL
                     FGDQHVHGLEGVRFYT"
BASE COUNT          348 a          626 c          709 g          373 t
ORIGIN      
        1 caactacgtt tcagtgacaa cgggggtaag gggcgagggc gatctcgaag gcccggagca
       61 cttccatgtg atcctcctcg atgccggccg cacgcgcctt gtcggcaccg atcttgagcc
      121 gatgctgcgc tgcatccgct gcggcgcctg catgaatcat tgccccgtct atcagagcgt
      181 cggcgggcat gtctacggct gggtgtatcc tggtcccatg ggttcggtgc tgacgccgag
      241 ctatgtgggg ctcgagaacg cgcgcgatct gccgaacgcc gcgaccttct gcaatcaatg
      301 cggcgtcgtg tgcccggtga agattccgct gccggatctc atgcggaaac ttcgcgagcg
      361 tgagttcgaa gagcgcttga agccgtggag cgagcgcatc gcgctcgccg catggagctg
      421 gctcgcgcag cgcccggcgc tctatgggtg gacggcaggg atcggtgctc gcgtgctcgc
      481 cgcgatgggc ggaaaatcga aattgataca caatctgccc gtgggccgcg gatggacgcg
      541 cgggcgtgac tttcccgcgc ccgaggggcg gacttttcgc gagctgtatg cttccagaga
      601 gaaaggcagg agaccaggat gaacaaaccg actgacagcg ccgtcgccac tgtcccgatg
      661 tgggtgaacg gcgcgaaaca ggcggcgaag agcacgcgct acggtgaggt gaccaatccc
      721 gcgacaggcg tcgtgacgaa gcgcgtgccc ttctgcgcgc gaagcgatat ccgcactgcg
      781 gtcgccgcgg cccaggccgc ttttcccaag tggcgtgata cgccgccgct caggcgcgcg
      841 cgcgttctca tgcgctatcg cgagctgctc gaggcccatc gcgaagaact ggcacgcctc
      901 atcaccgaag agcacggcaa gacgttgagc gacgcggcag ggtcggttca gcgcggcatc
      961 gaggtcgtcg agttcgcaat gggcatcccg catctcgtga aaggggagca cagcgaagaa
     1021 gtcggcaccg gcgtcgattg tcattcgctg cgccagccgc tcggcgtgtg cgtcggcatc
     1081 acgccgttca acttcccggt gatggtgccg ctctggttgt acccggtggc gattgcgtgc
     1141 ggcaacactt tcgtgctcaa gccctcggaa aaggtgccct cagcttcgat gcgcatggcg
     1201 gagctcttca cggaggccgg cctgccggac ggtgtcttca atgtggttca cggcgacaag
     1261 gaagcggtcg atgcgctgct cgagcatccc gacgtaaagg cggtgtcgtt cgtcggctcg
     1321 acgccgatcg caaagtacat ctatgagacg tgcgcaaaga acggcaagcg ggtgcaggcg
     1381 ctcggcggcg cgaaaaacca cgccgtcgtg ctgccggatg ccgatctcga tttcgcggcc
     1441 gatgcgctga tcggtgcggc gtacggctca gcgggtgagc gctgcatggc gatttccgcg
     1501 gtcgtcgcgg tgggcgacat cggcgaggcg ctcgttggca ggctgcgcga caaggcggcg
     1561 cggctcaaga tcggtcccgg aaccgacaag gatgtggaca tgggaccgct tgtcacccgc
     1621 gcgcaccgcg atcgcgtggc gggttacgtg gacacgggcg tcgccgaagg cgcgaagctc
     1681 gtgctcgacg ggcgcgcttt cgaggctgcg ggacgcgagg gttttttcct cggcgcgtcg
     1741 cttttcgatc acgtcacgcc cgacatgacg atttacaagg acgagatctt cggccccgtc
     1801 ctttccgtgt tgcgcgcgaa gacgctcgac gagtcgatcc acctcatcaa tgcgaatccc
     1861 tatgcgaacg gcacggcggt atttacgcgc tccggcgcgg ccgcgcgcaa gttcgagcgg
     1921 gaaatcgagg tcgggatggt ggggatcaac gtgccgattc cggttccgat cgcgttctat
     1981 tccttcggcg gctggcgctc gtctcttttc ggcgaccagc acgtgcacgg gctggaaggc
     2041 gtgcgcttct atacgc
//
LOCUS       DASURG010000552         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_299548, whole genome shotgun sequence.
ACCESSION   DASURG010000552 DASURG010000000
VERSION     DASURG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299548"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1635
                     /locus_tag="VFY80_09960"
     CDS             4..1635
                     /locus_tag="VFY80_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6008279.1"
                     /translation="MRESQDASCAILNRRGEVVAQHVVLPLHMGAFPACAAGVLKNYT
                     PDEVQEGDAFITNHPYLGGSPHAPDMAVLSPIFYQGEWVGFAANLAHKSDIGGPVPGR
                     CWSQAREVYQEGLHVPPIKYVQKYKTSKDIEALLGCNSRTPELVVGDLRGQVGAARLG
                     EKRFQEMMGRYGKQTILDSFEELFALTEAKVRAEIASWPDGVGEGERFVDSDGVELDK
                     PVRLHVRIEKKGDRLLFDLTGSADQTKGPANIRPTIVRAACCYLLTCLVDPALAINYG
                     LAKCIEIKVREGSVVNPRFPAPVNTYNPTVHALVESLLEASSQITPHKKVGDGCSSRS
                     IIIGGRSNKTGKGYVQYEIFGGGSGGRSGKDGVSGTNVNQSNAKIAPIEIIESEFAAR
                     VRRFELIRDSGGAGQFRGGLGFVREYEVLDNDARFSLRSTKHTVAPKGIDGGMDGKTG
                     LCTLNPGKPEERVIPSRYSDHVLHPGEAFSLETPGGGGLGDPLKRDPQRVLNDVRNGY
                     VSPRRAREIYRVSIEPVDGDFVLNEAETRALRSQA"
     gene            1757..>2430
                     /locus_tag="VFY80_09965"
     CDS             1757..>2430
                     /locus_tag="VFY80_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6008280.1"
                     /translation="MTIDPITLQVIQARLAGIVQEMQNSLFRTGYSTIIRESQDASCA
                     VLNTRGEVVAQHVVLPLHMGAFPACAAGILKSFAPDEIEDGDAFIINHPYLGGSPHAP
                     DMGVLTPIFYQSEWVGFAANMAHKSDIGGTVPGSGSGNAREIYQEGLHLPAVKYMSRR
                     QPVKEIDAIIASNSRTAAVVIGDLRGQVGAARLGERRIGELMDRYGKSTLLECTELLS
                     NYTEDRV"
BASE COUNT          478 a          770 c          755 g          427 t
ORIGIN      
        1 atcatccgcg agtcgcagga cgccagctgc gcgattttga atcgccgggg ggaagtcgtc
       61 gcgcagcacg tcgtgttgcc gctccacatg ggcgcctttc cggcttgtgc cgcgggcgtg
      121 ctcaagaact acacccctga cgaggtgcaa gaaggcgatg cgttcatcac caatcaccca
      181 tatctcggcg gcagcccgca cgcgccggac atggccgtgc tctcgcctat tttctatcag
      241 ggcgagtggg tcggtttcgc cgccaacctg gcgcacaaga gcgacatcgg cggtccggtg
      301 ccgggacgtt gttggagcca ggcacgcgag gtttatcagg aaggcttgca tgtgccgccg
      361 atcaaatacg tgcagaagta caagacgagc aaagacatcg aggcgctgct cggctgcaac
      421 agccgcacgc ccgagttggt tgtcggcgac ctgcgcggcc aggtcggcgc ggcgcgcctg
      481 ggcgagaagc gctttcagga gatgatgggt cgctacggca agcagaccat tctcgactca
      541 ttcgaagagt tgttcgcctt gacggaggcg aaggtgcggg ccgagatcgc gtcctggccg
      601 gacggcgtcg gcgaaggcga acgattcgtc gacagcgacg gtgtcgagct cgacaaaccg
      661 gtgcggcttc acgtgcgcat cgagaaaaag ggcgatcgcc tgctcttcga tctcaccggt
      721 tccgccgatc aaaccaaggg cccggcaaac atccgcccga cgatcgtgcg cgccgcctgc
      781 tgttacttgc tcacctgcct ggtcgatccc gcgctggcca tcaactacgg cctggcgaag
      841 tgcatcgaga tcaaggtacg cgaaggcagc gtggtgaacc cgcgcttccc cgcgccggtc
      901 aacacctata atccgacggt tcatgcgctg gtcgaatcgt tgctcgaagc ctccagccag
      961 atcacgccgc acaaaaaagt gggcgacggc tgcagcagcc gctccatcat tatcggcggc
     1021 cgcagcaaca agaccggcaa aggctacgtg cagtatgaaa tcttcggcgg cggctccggc
     1081 ggccggagcg gcaaagacgg cgtgtcggga accaacgtga accagtccaa tgccaagatc
     1141 gcgccgatcg aaatcatcga gtccgagttc gccgcccgcg tacgccgctt cgagctgatt
     1201 cgtgattccg gtggtgctgg ccaattccgc ggcgggctcg gtttcgtacg cgagtacgaa
     1261 gtcctcgaca atgacgcgcg cttctctctg cgctcgacca agcacacggt cgcccccaag
     1321 ggtatcgacg gcggcatgga cggcaaaacc gggctgtgca cgctcaatcc cggcaagccg
     1381 gaggagcgag tgattccatc gcgctattcc gatcacgtgc ttcaccccgg cgaagcgttc
     1441 agcctagaaa cgccgggcgg cggcggcttg ggtgatccgc tcaagcgcga tccgcaaaga
     1501 gtgctcaacg acgtgcgcaa cggttacgtc agcccgaggc gcgcgcgcga aatctaccgt
     1561 gtatccattg agccggtcga cggcgacttt gtgctaaatg aggcggagac ccgcgcgctg
     1621 cgctcgcagg cgtgaggcat gaggcttaag gcatgagtaa gggatccaag gctcatcctg
     1681 ccgacttgct cctacactgt tgcctcgtag ttgccgtatc tgctcacgcc tcgcgcgtca
     1741 cccctcacgc ctcacgatga ccatcgatcc catcaccctt caagtcatcc aggcgcgcct
     1801 ggccggcatt gtgcaggaaa tgcagaactc gctgttccgc accggctact cgacgatcat
     1861 ccgtgaatcc caggatgcca gctgcgcggt tttgaatacc cgaggcgagg tggtggcgca
     1921 gcatgtcgtg ctgccgctcc acatgggcgc gttccccgcc tgcgccgccg gcatcctgaa
     1981 aagctttgcg cccgatgaaa tcgaggacgg cgatgctttc atcatcaatc atccctactt
     2041 gggcggcagc ccgcacgcac ccgacatggg tgtgctcacg ccgatcttct accagagtga
     2101 atgggtcggc tttgccgcca acatggcgca caaaagcgat atcggcggta ccgtgccggg
     2161 cagcggatcg ggcaacgccc gggagattta tcaggaaggc ctccacttgc cggcggtcaa
     2221 gtacatgtcg cgccggcaac cggtaaaaga aatcgacgcc atcatcgcgt ccaacagcag
     2281 gacagcggcc gttgtcatcg gtgatctacg cgggcaggtg ggcgccgcac ggctgggcga
     2341 gcggcggatc ggcgaattga tggaccgcta cggcaagtcc acccttctcg aatgcaccga
     2401 gcttttgtcc aactatacgg aagaccgcgt
//
LOCUS       DASURG010000553         7200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1320069, whole genome shotgun sequence.
ACCESSION   DASURG010000553 DASURG010000000
VERSION     DASURG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320069"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..2263)
                     /locus_tag="VFY80_09970"
     CDS             complement(140..2263)
                     /locus_tag="VFY80_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6008281.1"
                     /translation="MGALPEQHQEKLVSTARTLARVLLDVLPDPAWVKDTAGRYIAVN
                     TAFRKVLQPLTQRNGEAIGCTDFDLHARDYAERAQREENEIMATRGAVRVQQCLQDAG
                     GRLRYFEVHRIALLDDAGNIEGTIGFAREVADKRGTGDGGHAHERELAALVENLPGMA
                     YRCYDEAGWTMDFVSEGCRALTGHAPQDFIANRARSWNAMIVLEDLERVWEERREQLR
                     ATGTFSVEYRIQHADGSLRWVWERAKRSPVGAGEPTAITGFITDITDTRHYLDELVYR
                     ATHDTLTGLANRTLLLDHLRHALAYGERYQRMVAVLVLNIDNFKYVNESLGHGAGDEL
                     LKEIAGRLRTTLREHDTISRIGADSFAMVLMDQDHLGSTSQVMSRLLNIVRKPMMLQS
                     QEVFVTCSVGCAMYPADAADPETLLRRADTAMHRARAQGRNCFYFYSADIDRKTEERL
                     HLEANLRRAAGRGELFLQYQPQLRLADNVPIGIEALVRWKHPELGVVSPARFIPLAEE
                     TDLIVSIGEWVLQEACTRTKSLLDAGLDLGHVAVNLSARQFRDRRLVARMRDILERTG
                     LAPGRLELEITESLVMHDVESVIGKLKELKALGILLAIDDFGTGYSSLSYLRRFPIDR
                     IKIDQSFTREVDSSSDAAAIARAVIQLGHALGLIVIAEGVESEGQLRFLKENGCDEIQ
                     GYIYARPLDPDKLTEVLRAPPQLPV"
     gene            2393..3067
                     /locus_tag="VFY80_09975"
     CDS             2393..3067
                     /locus_tag="VFY80_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6008282.1"
                     /translation="MKLYYHPVSTTCRPIMLFAAESTIALEFQLVDLFKGEHLGEAYC
                     SINPNGQVPTLEDGDFRLTESATILRYLAEKVESPAYPRDIRERARVNEMMDWLNTGL
                     YRDLGYGLIYPQIFPHLKRADAKVQAATIAWGRDRARKWLALLDRHFLGARPFVCGET
                     VTLADYLGSAFLTVGEVIRLDYTPWPNVQRWLERMKARPYWAKVNEGFYNHLVAPYAN
                     TVFEGL"
     gene            complement(3076..4749)
                     /gene="ettA"
                     /locus_tag="VFY80_09980"
     CDS             complement(3076..4749)
                     /gene="ettA"
                     /locus_tag="VFY80_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX6008283.1"
                     /translation="MAQYVYTMRRVGKVVPPKRVILEDISLSFFPGAKIGVLGLNGAG
                     KSSLLRIMAGVDRDYIGEATPMPGLRVGYLPQEPQLDASKDVRGNVEEGVQDTLGLIN
                     RFNAISERFAEPLEDEEMSRLLEEQGELQTKIEAAGGWELDRKLEVAADALRLPPWDA
                     DVSRISGGERRRVALCRLLLSEPDMLLLDEPTNHLDALSVQWLEQFLERYPGTVIAVT
                     HDRYFLDNVAGWILELDRGHGIPWQGNYSSWLEQKEKRLEIEARQESARIKAIQVELE
                     WVRANPKARQAKSKARLARFEELASAEVQKRNEVNEIYIPPGPHLGNLVIEAEALAKS
                     FGDRLLFDGLSFGLPPGGIVGVIGPNGAGKTTLFRILIGEERPDAGTLRIGDTVVMAY
                     VDQSRDALDPQKTVWEEISGGQDVIQVGNYASASRGYVARFNFKGADQQKRVSELSGG
                     ERNRLHLAKLLKSGGNVLLLDEPTNDLDVETLRALEQGLLDFPGCAVVISHDRWFLDR
                     VATHILAFEDDGRVVWFEGNYADYLEDRRRRLGAEADQPHRVRYRPLLA"
     gene            4764..5411
                     /locus_tag="VFY80_09985"
     CDS             4764..5411
                     /locus_tag="VFY80_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6008284.1"
                     /translation="MTEDSRSQPTLSRAGALCFRIFCGARALKWQPFFPESSMSPPSS
                     PGRRLCPAAIATLVLVLAACEQKPSTVTVKRADLQQTAAPEAPGRQSATTPDRSDVRA
                     LVAQARAPEPDKPRATPSESPPPRAAPPKTPTAAGKPAATRSVTDSELVARVKSAVLA
                     QPLSALMFNVSVANGVVTLSGTADSAETRDKAARAAADVAGVKSVENRINVLSGS"
     gene            5419..6159
                     /locus_tag="VFY80_09990"
     CDS             5419..6159
                     /locus_tag="VFY80_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX6008285.1"
                     /translation="MSNGAPAQAHFRFHEELAPFLAPSRRQCAFSHTCARAATLKHAI
                     ESLGVPHTEVGAIIVNGEPATLARIVRDGDTIEVGPPRAHPDPAGPVAFIADAHLGGL
                     ARLLRMLGLDTLYDNTVTDETILERAAEERRIILTRDRELLKRRAVLRGAFVHARKPD
                     AQLQEVSHRFALQQHVQPFSRCLHCNLRLQPVETAWAAARVPQRVAAHYRDFTRCPGC
                     ERIFWRGSHWARMRDMLDTAFSAPEIQA"
     gene            6263..>7200
                     /locus_tag="VFY80_09995"
     CDS             6263..>7200
                     /locus_tag="VFY80_09995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX6008286.1"
                     /translation="MADRSFAIDIQTMLVVMPVSAMLMASILWFAYAGRFRDGLARWT
                     TSLWTQALAWLLLATHDALLPDIAAKVMASSLLVAAAALQLAAIAEFRRQRVARQLLW
                     IAPVAALLPAVLTLDATPWQGIAGDVLYGACNFLIALAVLREPPHDARRARRLLALCY
                     ALATVALVVKAALAAEVGWVNIDNGPFYFGPYALIIAGSFAFLLMHKERADEESSRLA
                     TTDPLTGVFNRRTFIELAEQELARAHRAGTPLTIMMLDLDHFKQVNDTCGHLVGDEVL
                     VSFTRLIRDCIRRGDLVVRYGGEEFCVLLPSTTLAAA"
BASE COUNT         1232 a         2357 c         2242 g         1369 t
ORIGIN      
        1 cgccttgggt acgctcggtg gtgccgcggc tggaggattt atcggtcacc agtatggcga
       61 cgacattcgc cgtagagttg actagccgaa cagggcaaac agggaagacc gggagcaatc
      121 ccggtctttt ttgttcgtgt cacaccggca gctgcggcgg cgccctcagg acttcagtta
      181 gcttgtcggg gtccagcgga cgcgcataga tgtatccctg gatttcatcg cagccgttct
      241 ctttgaggaa tcgcagctgg ccttcgcttt cgacgccttc cgcgatgacg atgagaccca
      301 atgcatggcc gagctggatc acggcacgtg cgatggcggc cgcatccgaa gagctgtcca
      361 cttcgcgcgt gaacgactgg tcgatcttga tgcgatcgat gggaaagcgg cgcaggtagc
      421 tcaagctgga atagccggtc ccgaaatcgt cgatcgcgag cagtatcccg agggccttca
      481 gttccttcag ctttccgatc accgattcga cgtcgtgcat gaccaggctc tcggtgatct
      541 cgagctcgag ccgcccggga gcgagaccgg tgcgctcgag gatgtcccgc atgcgagcga
      601 cgagccggcg gtcacgaaac tgacgcgccg acagattgac tgcgacgtgg cccaggtcga
      661 gtccggcatc gagcagcgac ttggtgcggg tgcaggcttc ctgcagcacc cattccccga
      721 tgctcacgat gaggtcggtt tcctccgcaa gcggaatgaa acgcgcgggt gatacgacac
      781 ccagctcggg atgcttccag cgcaccagtg cctcgatgcc gatcgggacg ttgtcggcca
      841 ggcgcagttg cggctgatat tgcaggaaga gctcgccccg tcctgccgcc cggcgaagat
      901 tggcttcgag gtgcagacgt tcctcggtct tgcgatcgat gtccgccgag tagaaataga
      961 agcagttacg tccctgcgcg cgtgcgcggt gcatggcggt atcggcgcgg cgcagcaacg
     1021 tttccggatc cgccgcatca gcgggataca tcgcgcaccc gacgctgcag gtcacgaaca
     1081 cctcctggga ctgcagcatc atgggcttcc gtacgatgtt gagcagccgg ctcatcacct
     1141 gggacgtgga gcccaggtga tcctgatcca tgagcaccat ggcgaatgaa tcggcgccga
     1201 tgcgcgagat cgtatcgtgc tcgcgcaatg ttgtgcgcag ccggcccgca atctccttca
     1261 gaagctcgtc tccggcgccg tgccccaggc tttcgttgac gtacttgaaa ttgtcgatgt
     1321 tgagcaccag cacggctacc atgcgctggt agcgctcgcc gtacgcgagc gcgtgtcgca
     1381 ggtgatcgag cagcagcgtg cggttcgcga gcccggtgag cgtatcgtgc gttgctcgat
     1441 acacgagctc gtcgagatag tgccgcgtat cggtgatgtc ggtgatgaaa cccgtgattg
     1501 cggtgggctc gccggcaccc accggagagc gcttcgcgcg ctcccacacc cagcgcaggc
     1561 tcccatcggc atgctggatg cggtattcaa cggaaaacgt gccggtggcg cgaagctgtt
     1621 cgcgccgctc ctcccacacc cgctccagat cctcgagcac gatcatggca ttccacgatc
     1681 gtgcacggtt cgcgatgaag tcctgcggcg catgaccggt cagcgcgcgg catccttcgc
     1741 tcacgaaatc catcgtccat cctgcttcgt cgtaacagcg gtacgccatg ccgggaaggt
     1801 tctcgacgag cgcggccagt tcgcgctcgt gcgcgtgccc tccgtctccc gtgccacgct
     1861 tgtcggccac ttcacgcgca aacccgatgg tgccttcgat gttgccggca tcgtccagca
     1921 aggcgattct gtgcacttca aagtagcgca gcctcccgcc cgcatcctgc agacactgct
     1981 gaacccgcac cgcgccgcgc gtagccatga tctcgttttc ctcgcgctgc gcccgctcgg
     2041 cgtagtcgcg cgcatggaga tcgaagtccg tgcagccgat cgcctcgccg ttgcgctgag
     2101 tgagcggttg caagaccttt ctgaaggcgg tattgacggc gatgtagcgt cccgccgtgt
     2161 ctttcaccca ggcaggatcc ggcaggacat cgagtaggac acgcgcaagg gtgcgcgctg
     2221 tgctgacgag cttttcctgg tgctgctccg gcagtgcgcc cattcacctc ccctggttgg
     2281 cgtcgccggc ggttattatg cctcgtgaac gctcatcgcg atagtcggtg tattgccgac
     2341 agcagcgccg ttgcctaaga tggatcgcca tggctctcgc ccggggaaca tcatgaagct
     2401 ttattaccat ccggtctcaa ccacctgccg tcccatcatg ctgttcgccg ccgaaagcac
     2461 gatcgcgctc gagtttcagc tcgtcgatct tttcaagggc gagcatctgg gagaggcgta
     2521 ttgcagcatc aatcccaatg gtcaggtgcc gacactcgaa gacggcgact tccggttgac
     2581 cgaaagcgcc acgatattga ggtatcttgc agagaaggtg gaatcgccgg cctatccccg
     2641 agacattcgc gagcgggcgc gggtaaacga gatgatggac tggctcaata ccgggctcta
     2701 tcgcgacttg ggctacggcc tcatctatcc acagatcttt ccgcatctca agcgcgctga
     2761 cgcgaaggtt caggcggcca ccatcgcatg gggacgcgac cgggcgcgca agtggctcgc
     2821 gctcctcgac cgccattttc tcggtgcccg gcctttcgtc tgcggcgaaa ccgtgacgct
     2881 cgcggattat ctcggatcgg cctttctcac ggtcggcgag gtcatccggc tcgattacac
     2941 gccgtggccc aatgtgcagc gttggctcga acgcatgaag gcgcgaccgt actgggccaa
     3001 ggtgaacgag ggcttttaca atcatctcgt cgcgccctac gcgaataccg tgttcgaggg
     3061 cctctgaccg cagcgtcagg cgaggagcgg ccgatagcgc acgcgatgcg gctgatcggc
     3121 ttccgccccc aggcgcctgc ggcggtcctc gaggtagtcg gcgtagtttc cctcgaacca
     3181 caccacgcgg ccatcgtcct cgaaagcaag gatatgggtc gcgacgcggt cgaggaacca
     3241 gcggtcgtgg gagatcacga ccgcgcatcc cggaaaatcg agcagcccct gctccagcgc
     3301 ccgcagcgtc tcgacgtcga gatcgttggt cggctcgtcg agcagcagga cgtttccgcc
     3361 gcttttcaat agttttgcga ggtgcaggcg attgcgctcg ccgccggaaa gctcacttac
     3421 gcgcttttgc tggtcggcgc ccttgaagtt gaagcgtgcc acatagcccc ggctggcgct
     3481 ggcgtaattg cccacctgga tcacgtcctg gccacccgag atttcctccc agaccgtttt
     3541 ctgcggatcg agcgcgtccc ggctttgatc gacgtacgcc atgacgactg tgtcgccgat
     3601 gcgcaaagtg cccgcgtcgg gccgctcctc gccgatcaga atgcggaaga gcgtcgtctt
     3661 ccccgccccg ttcggtccga tcacgcccac gatgccgccg ggtggcaacc cgaaagagag
     3721 cccgtcgaat agcagccggt caccgaacga tttcgccagg gcttccgcct cgatcaccag
     3781 gttcccgaga tgggggccgg gcgggatgta gatctcgttc acctcgttcc gcttctggac
     3841 ttcggcggat gcgagctcct cgaagcgggc aagccgtgcc ttgctcttcg cctgcctggc
     3901 cttcgggttg gcacgcaccc attcgagttc gacctggatt gccttgatgc gcgccgattc
     3961 ctggcgcgcc tcgatttcga ggcgcttctc cttctgctcg agccacgacg aatagttgcc
     4021 ctgccacgga atgccgtgcc cgcgatcgag ctcgagtatc catcccgcca cgttgtccag
     4081 gaaatagcgg tcgtgcgtga cggcgatgac cgtgcccggg tagcgctcaa gaaactgctc
     4141 cagccattgc accgagagcg cgtccaggtg attggtcggc tcgtcgagaa gcagcatgtc
     4201 gggctccgag agcaatagcc gacacagtgc cacgcgccgc cgctcgccgc ccgaaatgcg
     4261 gctgacatcg gcatcccagg ggggaagccg caatgcgtcg gccgcgactt cgagcttgcg
     4321 gtcgagctcc cagccaccgg ccgcttcgat cttcgtctgt agctccccct gctcttccag
     4381 aagccgggac atctcctcgt cctcaagcgg ctcggcaaag cgttcgctga tggcattgaa
     4441 gcggttgatg agcccgagcg tgtcctggac accctcttcc acattgccgc gcacgtcctt
     4501 cgatgcgtcg agctgcggct cctgcgggag gtagccgacg cgcagccccg gcatgggggt
     4561 cgcttcaccg atgtaatccc gatcaacacc ggccatgatg cggagaaggc tcgacttgcc
     4621 cgcgccgttc agtccaagca cgccgatttt cgcgccggga aagaacgaga gcgagatgtc
     4681 ctcgaggatg acgcgcttgg gcggaacgac cttgcccacg cggcgcatgg tgtagacgta
     4741 ctgagccatg atgtcggagc aaagtgacag aggatagccg aagccagccg acgttgtcgc
     4801 gcgccggagc gctctgcttt cgaatttttt gcggcgccag ggctctaaag tggcaaccct
     4861 tcttcccgga gtcttccatg agccccccat cttctcccgg ccgccgcctg tgcccggcgg
     4921 cgattgcgac gctcgtcctc gtactggccg cgtgcgagca aaagccttcc accgtcacgg
     4981 tgaagcgcgc cgatttgcag cagacagcgg caccggaagc gccggggcgc caaagcgcca
     5041 caactccgga tcgcagcgac gtgcgcgcgc tcgtcgcaca ggcgcgcgct ccagaacccg
     5101 ataagccgcg cgccacgccg agcgaatcgc cgcctccgag ggctgcgccg ccaaaaacgc
     5161 ccaccgccgc aggcaagccg gcggcgaccc gtagcgtgac cgactctgag cttgttgcgc
     5221 gcgtcaaatc cgccgtactc gcccagccgc tgtcggccct gatgttcaac gtgagcgtcg
     5281 caaacggtgt ggtgacgctc tcgggtacgg cggattctgc ggagacgcgc gacaaggccg
     5341 cacgcgcggc agcagatgtg gccggcgtga agtcggttga aaaccggatc aacgttctga
     5401 gcggttcata atgccgcaat gagtaacggt gcgccggcgc aggcacactt ccggtttcat
     5461 gaggagctgg cgcctttcct ggcgccgtca cggcggcagt gcgcgttctc acatacgtgt
     5521 gcacgcgcag cgacgctgaa gcacgccatc gagtcgctgg gcgttccaca taccgaggtg
     5581 ggcgcgatca tcgtgaacgg ggagcccgca acgctcgccc gcatcgtgcg cgacggggac
     5641 acgatagagg tgggcccgcc ccgtgctcac cctgatccgg ccgggccggt cgcgttcatc
     5701 gccgacgccc atctcggcgg cctggcgcgg ctcctgcgca tgctgggtct tgatacgctc
     5761 tatgacaaca cagtcacaga cgagacgatc ctggaacggg ccgccgaaga gaggcgcatc
     5821 atcctgacac gcgatcgcga actgctgaag cgccgagcag tcctgcgtgg tgcgttcgtg
     5881 catgcacgca agcccgatgc gcaattacag gaggtctcgc accgcttcgc tctgcagcag
     5941 cacgtacagc cgttcagccg ttgcctgcac tgcaatttgc ggctgcagcc ggtggaaaca
     6001 gcgtgggccg cagcgcgcgt tccccagcgc gtagccgcgc actaccgcga tttcacccgc
     6061 tgcccgggat gcgagcgcat tttctggcga ggctcgcatt gggcccgcat gcgcgacatg
     6121 ctcgacaccg cgttctccgc gccggagatc caggcatgac gttgccatag cgcaacgtgc
     6181 gcatgccctg cgtataattt ctcagcccac cgggaatttc atcgcatcgg gatgggaata
     6241 cgcgtccagg ggatagcgct gcatggccga ccgttcgttc gcaatcgaca tacagacgat
     6301 gctcgtcgtc atgccggtga gcgcgatgct gatggcctcg attctgtggt tcgcctacgc
     6361 gggtcgcttc cgggacggac tcgcgcggtg gaccacttcg ctctggacgc aggcgctggc
     6421 gtggctgctg cttgcgactc acgacgcgct gcttccggat attgccgcga aggtcatggc
     6481 gagcagcctg ctggtcgcag cagcagcgct tcagctggcg gcgatcgccg aatttcgccg
     6541 gcagcgagtc gcgcgtcagc tactgtggat tgcgcccgtg gcagcgctgc ttcccgccgt
     6601 tttgacgctg gacgcgacgc cctggcaggg catcgcaggg gatgtcctgt acggggcgtg
     6661 caattttctg atcgcgctgg ccgtcctgcg cgagccccca catgacgcac gtcgcgcccg
     6721 caggctgctt gcgctgtgct atgcacttgc gaccgttgcg ctcgtcgtga aagcggcgct
     6781 cgctgccgaa gtcggctggg tcaatatcga caacggccct ttctatttcg gtccctatgc
     6841 gctcatcatc gcaggctcct ttgccttcct gctcatgcac aaggaacgtg ccgacgagga
     6901 atcgagccgg ctcgcgacca ccgaccctct gaccggcgtc ttcaaccggc gcacgttcat
     6961 cgagctggcg gaacaggagc tcgcgcgcgc tcaccgcgcc ggtacaccgc tcaccatcat
     7021 gatgctcgat ctcgaccact tcaagcaggt aaatgacacc tgcgggcatc tcgtcggaga
     7081 tgaagtgctg gtctcgttta cccgcctcat ccgcgactgt atccgccgcg gcgaccttgt
     7141 ggtccgctac gggggtgagg aattctgcgt attgctgcct tccaccaccc tcgccgccgc
//
LOCUS       DASURG010000554         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_400671, whole genome shotgun sequence.
ACCESSION   DASURG010000554 DASURG010000000
VERSION     DASURG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400671"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFY80_10000"
     CDS             <1..411
                     /locus_tag="VFY80_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6008287.1"
                     /translation="NAHYRDMRDLLAQSDFVVLQVPYSQETHHLIGTAELAMMKPTAV
                     LINSTRGGVVDDAALIAALKAGTIRGAGLDVFENEPKLNPRFLELRNVVLAPHIGSST
                     EATRRAMAMTAAANLVAALRGEAPPNLVNPEAGK"
     gene            483..1442
                     /locus_tag="VFY80_10005"
     CDS             483..1442
                     /locus_tag="VFY80_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX6008288.1"
                     /translation="MSYFIQLFNLMIEVMLPLTLLVAVGGLWASFFPDTPVEGARTLL
                     NRMVMYLFLPCILFSVASATPISADLLSVPLLVGIGSLTCGAVLYGLMYLTPVGRGIP
                     DATRAILVVGGMFGNTFNVGVPVLVFFFGSGATRYAVFNDMLMTMPLVWSVGVWICTR
                     LGAHRSAERPAVWRVMLSMPPIWAFILGTGSQQLGLAYAPLTAATGFIGQATIPVTLF
                     VLGMTIPWRNLTPRPEILAAAAVKLIAAPVIVWFAAKLMYSPMGEAQHAAVVEGTTPA
                     MVTTLLLADRFRLDSRAAALLIGWTTISFWLTLPLIMALGLIR"
     gene            1465..2346
                     /locus_tag="VFY80_10010"
     CDS             1465..2346
                     /locus_tag="VFY80_10010"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6008289.1"
                     /translation="MKLGFIGLGAMGRPMALNLMKRGHEMTVFARRPASAQALVAAGA
                     RLCPTPTEVATRAEVVFTMVTHSSDVEEVVLGKDGAIHGAASGSVVVDMETIAPGVAR
                     SVAQALEDKGVDMLDAPVSGGPMGAEEATLSIMVGGKQAVFERVKPLFDCLGKTVVHM
                     GDHGAGQVTKACNQLALLVTAQASAEALTLARACGLDPARVREVMMGGIAASRVLEFF
                     GKRMADRDFEAGIESRLYHKDLNIVLALAHERGIALPAAALTMQHVNALVGRGEGRND
                     LSALIRIVEGMGARRPS"
     gene            2343..>2926
                     /locus_tag="VFY80_10015"
     CDS             2343..>2926
                     /locus_tag="VFY80_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX6008290.1"
                     /translation="MSFTSIEQAPARLQRSELAVPGSMAALFDKAAKSAADVVFLDLE
                     DAVAPDDKVEARRNVVAALNDIDWGERTLSVRINGLDTPYMYQDVIDLVENCPRLDLL
                     LIPKVGVPADVYAVDVLVTQIEQAKRRTKHVGLEVLIETALGWSNVEAIARASRRLEA
                     MCFGSGDFAASMRMRTTTIGGLNPEYGVLTDAAS"
BASE COUNT          509 a          903 c          970 g          544 t
ORIGIN      
        1 aacgcacact accgcgacat gcgggatctg cttgcgcagt cggatttcgt cgtgctgcag
       61 gtgccgtatt cgcaggagac gcatcacctg atcggcactg cggaactcgc gatgatgaag
      121 cccaccgcgg tcctgatcaa ctcgacacgc ggcggcgtgg tggacgatgc ggcattgatt
      181 gcggcgctga aggccggaac gatacgcggg gcaggactcg acgtgttcga aaacgagccc
      241 aagctcaacc cgcgattcct ggagctcagg aacgtcgtgc tggctccgca tatcggcagc
      301 tccacggagg cgacgcgacg cgcgatggca atgaccgctg cggccaatct ggtggctgcg
      361 ctgcgcggcg aagcgccccc gaacctggtg aatcccgaag caggaaaata gatcgcgcaa
      421 ccgcgtgtgt tgatctaaag ctcgcgggca cgctcgccct gcggtacggt tgttccactg
      481 ccatgagcta cttcattcaa ctcttcaacc tcatgatcga ggtgatgttg ccgctgacgc
      541 tgctcgttgc ggtgggcggg ctgtgggcaa gcttctttcc cgacacgccg gtcgagggag
      601 cgcgcaccct gctaaaccgc atggtgatgt acctctttct gccctgcata ctgttctccg
      661 tcgcctccgc cactccaatc tccgcggacc tgttgtcggt gccgctactg gtcggcatag
      721 gctcgctcac gtgcggcgcg gttctctatg ggctcatgta tctcacgccg gtcgggcgcg
      781 gaatccctga cgcgacgcgc gcaatcctgg tcgtaggcgg catgttcggc aatacgttca
      841 acgtcggggt cccggtgctc gtgttttttt tcggcagcgg tgcaacgcgc tatgcggtct
      901 tcaacgacat gctgatgaca atgccgctcg tgtggagcgt cggcgtctgg atctgcacgc
      961 ggctcggcgc gcaccgcagc gccgagcgtc ccgccgtatg gcgcgtcatg ctctcgatgc
     1021 cgccgatctg ggcgttcatt ctcggcacgg gatcgcagca gctggggctt gcctatgcac
     1081 cgcttacggc ggcgaccggc ttcatcggtc aggcgaccat accggtcacg ctgtttgtgc
     1141 tcggcatgac cattccatgg cggaacctga cgccgcgacc cgagatcctc gcggcggcag
     1201 cggtgaagct gatcgcggcg ccggtcatcg tctggtttgc ggccaaactg atgtacagtc
     1261 cgatgggcga ggcgcaacat gcggccgtgg tggaagggac cacccccgcc atggtgacga
     1321 cgttgctgct cgcggatcgc ttccggctcg actcccgggc agcagcgctg ctgatcggct
     1381 ggacgacaat ttcgttctgg ctcacgctgc cgctcatcat ggcgctcggg ttgattcgtt
     1441 agtgcaaacg caaaaaggaa tgacatgaaa cttgggttca tcggtcttgg cgccatgggt
     1501 cgacccatgg cgctcaacct catgaagcgt ggacacgaga tgaccgtatt cgcccggcgc
     1561 ccggcctctg cgcaagcgct cgtcgccgcg ggtgcgcggt tgtgccccac gcccaccgag
     1621 gttgcgacgc gtgccgaagt cgtcttcacc atggtgacgc actcgagcga cgtggaagag
     1681 gtagtgctgg ggaaagacgg tgcgattcac ggcgccgcat ccgggagcgt cgtcgtcgac
     1741 atggagacca tcgcccccgg ggtcgcgcgc agtgtcgcgc aggcgctcga agataagggc
     1801 gtcgacatgc tcgacgcccc ggtctccggc gggccgatgg gcgcggagga ggcgacgctg
     1861 tcgatcatgg tcggcggaaa gcaagcggtc ttcgagcgtg tgaagccgct tttcgactgt
     1921 ctcggcaaga ccgttgtcca catgggggat cacggcgcgg gccaggtgac aaaggcgtgc
     1981 aatcagctcg cgttgctcgt cacagcgcaa gcaagcgccg aagcgctcac gctcgctcgg
     2041 gcgtgcggcc tcgatcccgc ccgggtgcga gaagtgatga tgggtggcat tgcagcaagc
     2101 cgcgtgctcg aattcttcgg caagcgcatg gccgaccgcg acttcgaagc gggcatcgaa
     2161 tcgcgcctct atcacaagga tctgaacatc gtgctggcgc tcgcgcacga gcggggtatt
     2221 gcgctcccgg cggctgcgct taccatgcag catgtcaacg cgctcgttgg gcggggcgaa
     2281 ggaagaaacg atctctccgc gctgatccgg attgtcgagg gcatgggtgc gaggaggccg
     2341 tcatgagctt caccagcatt gagcaggcgc cggcaagact tcagcgctcc gagctcgccg
     2401 ttcccggttc catggcggca ctcttcgaca aggcggcgaa atcggccgcc gacgtggtct
     2461 ttctcgatct cgaggacgca gtggcgcccg acgacaaagt cgaggcccgc aggaacgtcg
     2521 tcgccgcgct caacgacatc gactggggcg aaagaacgct ttcggtgcgg atcaatgggc
     2581 tcgatacgcc ctacatgtat caggatgtca tcgatctggt cgaaaactgc ccgcgtctcg
     2641 acctgttgct cattccgaaa gtgggcgtgc cggcggacgt gtacgcggtc gatgtgctcg
     2701 tgacccagat cgagcaggcg aaacggcgca cgaagcacgt cggcctcgag gtgctgatcg
     2761 aaaccgcgct cggctggagc aacgtggaag ccattgcacg agcaagccgg cggctggaag
     2821 cgatgtgctt cgggtccggc gacttcgcgg cctcgatgcg catgcgaacg acgacgatcg
     2881 gcggcctcaa ccctgaatac ggtgtgctga ccgatgcggc gtcgga
//
LOCUS       DASURG010000555         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1013547, whole genome shotgun sequence.
ACCESSION   DASURG010000555 DASURG010000000
VERSION     DASURG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013547"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFY80_10020"
     CDS             <1..656
                     /locus_tag="VFY80_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6008291.1"
                     /translation="FGGVEIARRGIHVLAIDGPGQGEALRLQGIPSRYDYEVPARAAL
                     DYMTSRPDVDPQRIAVMGFSMGGYYAPRAAAMEPRFAACVAWGGHFDYHEAWVRRRKI
                     MESGGTKISAPAFQLPWVLGMPDMEACMKKLENYRLAGVAEKIRCPFYCIHGENDNIV
                     PLEFAQRLYEAVGSKDKTLKVLTAHDGGSDHCQEDNRQVGANLVADWLAQRLRGQLA"
     gene            complement(743..1576)
                     /locus_tag="VFY80_10025"
     CDS             complement(743..1576)
                     /locus_tag="VFY80_10025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6008292.1"
                     /translation="MHPMLNIAVKAARRAGNIINRATRNLDVVSVREKVANDYVSEVD
                     HEAEQAIIRTLHEAYPDHSILAEESGASGNSEYQWIVDPLDGTTNFLHGFPQYAVSIA
                     LMHRGIVTQAVIYDPSRNDLFTSTRGRGAFLNETRIRVSKRIQMKPSLIGTGFPFRQL
                     EHVDAYLAMLRDVMHNSAGVRRAGAAALDLAYVAAGRLDAFWEIGLSKWDMAAGALLI
                     IEAGGLVGDLEGNPGYLDSGNILAGNPKIFGQMVQLLARHLTPPLRSRTLVIPVKTGI
                     Q"
     gene            1595..2329
                     /locus_tag="VFY80_10030"
     CDS             1595..2329
                     /locus_tag="VFY80_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX6008293.1"
                     /translation="MNKEQALKNIRIVLVGTTHPGNIGASARALKTMGLARLYLVAPE
                     RYPDPEAQWRASRATDVLASAKVCATLDEALAGVTYAVACSARSREIAVPETTAREAA
                     KEVIDIAQTQEAALVFGNETFGLTTDEVNKCRLLATIPAAADYSSLNLAAAVQVFAYE
                     LRMAAFAESAAAPAAKPRDFATHEEVEGFYAHLEHAMVEVGFLDPQHPKKLMPRIRRL
                     FARAQLEKEEVNILRGILKSFRNRER"
BASE COUNT          455 a          738 c          788 g          440 t
ORIGIN      
        1 tcttcggcgg cgtcgagatc gcgcggcgcg gcattcacgt gctggcgatc gacgggccgg
       61 ggcagggcga agcgctgcgg ttgcaaggca ttccgagccg ctacgattac gaggtgccgg
      121 cgcgtgcggc gctcgattac atgacgagcc gtccggacgt ggatccgcag cgcatcgccg
      181 tgatgggctt cagcatgggc ggctactacg cgccgcgtgc ggcggcaatg gagccgcgct
      241 tcgctgcgtg cgtggcgtgg ggcgggcact tcgattatca cgaggcgtgg gtgcggcggc
      301 ggaagatcat ggagtccggc ggcacgaaga tctcggcgcc tgcgttccaa ttgccatggg
      361 tactcggcat gccggacatg gaggcgtgca tgaagaagct ggagaactac cggctcgccg
      421 gcgtggcgga gaagatccgg tgcccgttct attgcatcca cggcgagaac gacaacatcg
      481 tgccgctcga attcgcgcag aggctttacg aagcggtggg ctctaaggac aagacgctga
      541 aggtgctcac tgcgcacgac ggcggcagcg atcactgtca ggaagacaat cggcaggtcg
      601 gcgctaatct ggtcgccgat tggctcgccc aacgcctgcg cggccaactc gcctaatacg
      661 ccgtagatcg tcttcccggc gaaagtcccg aataggagat cgtcttcccg gcgcaagccg
      721 ggatccagac gctttgctta cttcactgga ttccggtttt taccggaatg acgagcgtcc
      781 gcgagcgcag cggaggagta agatggcgcg cgaggagctg caccatctgc ccgaagatct
      841 tcggattgcc ggcgagaatg ttgccgctat cgagatatcc cggattgccc tcgagatcgc
      901 cgaccagccc gcccgcctcg atgatcagca gcgcgccggc cgccatgtcc cacttgctta
      961 ggccgatttc ccagaacgcg tcgagccgtc ccgccgcgac gtacgcgaga tcgagcgccg
     1021 ccgcgcccgc gcgccgcacg ccagcactgt tgtgcatcac gtcgcgtagc atcgcgagat
     1081 acgcatcgac gtgctcgagc tgccgaaaag gaaaaccggt gccgatcagg ctcggcttca
     1141 tctggatgcg cttgctcacg cggatgcgcg tttcgtttaa gaacgcaccg cgcccgcgag
     1201 tggacgtgaa gagatcattg cggctcggat cgtagatcac cgcctgggtg acgatgccac
     1261 ggtgcatgag cgcgatcgaa accgcatact gcggaaagcc gtgcagaaag ttggtcgtgc
     1321 cgtcgagcgg atcgacgatc cactgatact ccgaattccc agaggcgccg ctctcctctg
     1381 ctagaatcga gtggtcggga tacgcctcgt gcagcgtccg gatgatcgct tgctccgctt
     1441 cgtgatcgac ttccgacaca taatcgttcg cgaccttctc gcgcaccgac acgacgtcga
     1501 gattgcgcgt ggcgcgattg atgatgttgc ccgcgcggcg cgccgctttc acggcgatat
     1561 tgagcatcgg atgcatatca gggcggcatt gtaagtgaat aaggaacagg cgctcaaaaa
     1621 tatccggatc gtgctggtcg gtacgacgca tcccggcaac atcggggcga gtgcgcgcgc
     1681 gctgaagacg atgggactcg cgcggctcta tctggttgca ccggagcgct atcccgatcc
     1741 cgaagcgcag tggcgcgcct cgcgcgcgac ggacgtgctt gcgagtgcga aggtgtgcgc
     1801 gacgctcgat gaggcactgg ccggcgtcac atatgcggtc gcgtgttccg cacgaagccg
     1861 cgaaatcgcg gtgccggaga cgacggcccg tgaagcggcg aaggaagtga tcgacatcgc
     1921 gcaaacccag gaggctgccc tcgtcttcgg caacgaaacc ttcggcctca cgaccgatga
     1981 agtgaacaaa tgccggctct tggcgacgat tcccgcggcg gcagattact cctcgctcaa
     2041 cctcgccgcg gccgtgcagg tcttcgccta tgagctgcgc atggcggcgt tcgctgagag
     2101 cgctgctgct cctgctgcga agccgcgcga tttcgcgacg cacgaggaag tcgagggctt
     2161 ttacgcgcat ctcgagcatg cgatggtgga agtcggcttt cttgatcctc agcacccgaa
     2221 gaaactgatg ccgcggatac gccggctttt tgcgcgcgcg caactcgaaa aggaagaggt
     2281 caacatcctg cgcggcatcc tcaagtcctt tcgcaaccgc gagaggtagc ctcgtccaag
     2341 cagggcatct tcccggcgaa cgccgggatc cagacgtttt gctttacttc actggattcc
     2401 ggttttcacc ggaatgacga g
//
LOCUS       DASURG010000556         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_196417, whole genome shotgun sequence.
ACCESSION   DASURG010000556 DASURG010000000
VERSION     DASURG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196417"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFY80_10035"
     CDS             <1..936
                     /locus_tag="VFY80_10035"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX6008294.1"
                     /translation="RPLIAKHLVEIARRTGADAISHGATGKGNDQVRFELGAYALMPD
                     VRIIAPWREWDLTSREKLLAYAKVHRIPVDYGRKKGAAPYSMDANLLHISYEGGILED
                     PRYEPEESMWRITVSPEKAPGRPEYVELAYSNGDIVAVNGRKMTPGQILAKLNQLGGK
                     HGIGRLDLVENRYVGMKSRGCYETPGGTIMLKAHRAMESITLDREVLALKDDLMPRYA
                     RLIYNGYWFSPERRLLQTLIDASQQPVNGIVRVRLYKGTVMTVGRESKTHSLYDPAIA
                     TFEDDKGAYDQADAAGFIRLNALRMRIAAKLNKRK"
     gene            939..1517
                     /locus_tag="VFY80_10040"
     CDS             939..1517
                     /locus_tag="VFY80_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008295.1"
                     /translation="MTTIAVVRKNGYAAIAADTMTKWGTGKETAEYIINHSKILKVGN
                     TYIGVTGSATFKTILQDYFGRSRTYARFDTPFAIFKTWHKLHAALKQEYYLVTGHSEE
                     DVLESSRMDALIVNARGIFGVAAHRTVQEFSKFYAFGSGGDFAMGSMHSTYAEAGRSA
                     ESVARAAIEAAAEFDDSTGVPVDCFTVKLARR"
     gene            1534..2025
                     /locus_tag="VFY80_10045"
     CDS             1534..2025
                     /locus_tag="VFY80_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX6008296.1"
                     /translation="MPSFDIVSEVNQVEVQNAVDQCNKEIANRFDFKGSDARVEINEK
                     EKKLSAYADDDFKLGQLRDVLTGKLAKRGVDVRCLELAKIEKVSGNRVKQDITVRVGV
                     DQDLGKKIVKLIKDSKLKVQSSIQGDAVRVSGSKKDVLQETIALVRKSITDFPLQFKN
                     FRE"
     gene            complement(2075..>2589)
                     /locus_tag="VFY80_10050"
     CDS             complement(2075..>2589)
                     /locus_tag="VFY80_10050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX6008297.1"
                     /translation="ALALIRRAVAEATPVIGHCLGGQLMAKAMGGAVARNRVKEIGWG
                     AVEVLPGALAAEWFGGSVTAFNAFHWHGEAFSVPPGATRLASSAYCENQMFVLGPHLA
                     MQCHVEMTKEMIVTWCKDWEKEVEALAARVPSVQTPADMTSRLPARLPALHAIADRLY
                     ERWITRLRAD"
BASE COUNT          550 a          810 c          800 g          429 t
ORIGIN      
        1 cgccccctca tcgccaagca tctcgtggag atcgcccgcc gcactggcgc ggatgccata
       61 tcgcatggcg ccacaggcaa gggcaacgat caagtcaggt tcgaactcgg cgcctacgct
      121 ttgatgcccg atgtgaggat cattgcgccg tggcgcgaat gggatctcac ctcgcgcgag
      181 aaactgctgg cgtatgcgaa ggtacaccgc atcccggtgg actacggcag gaagaaaggt
      241 gcggcgccct attcgatgga tgccaacctc ctgcacatct cctatgaagg cggcatactc
      301 gaggatccgc gctacgagcc ggaggaatcg atgtggcgca tcacggtgtc gccggaaaag
      361 gcgcccggtc ggcctgaata cgtggagctc gcctattcga acggcgacat cgtggcggtc
      421 aacggccgga aaatgacacc ggggcagata ttggcgaagc tcaatcagct cgggggcaag
      481 cacggcatcg gccggctcga cctcgtcgaa aaccgctacg tcggcatgaa atcgcgcggc
      541 tgttacgaga cgcccggagg cacgatcatg ctgaaagccc accgtgcgat ggaatcgatc
      601 acgctcgacc gcgaggtgct cgccctcaag gacgatctca tgccgcgcta tgcgcgcctc
      661 atctacaatg gctactggtt cagtcccgag cgtcggctgc tgcagacgct cattgacgcc
      721 tcgcagcagc cggtcaacgg catcgtgcgg gtaaggctgt acaagggtac tgtcatgacg
      781 gtcggccgcg agtccaagac ccattctctg tacgatccgg ccattgccac cttcgaggac
      841 gacaagggcg cctacgacca ggccgacgct gcgggcttca tccggctcaa tgcgctgcgc
      901 atgcgcattg ccgccaaact caacaagcgg aaatagcgat gacgacgatt gcggtggtca
      961 ggaagaacgg ttacgcggcg atcgccgcgg acacgatgac caagtggggc accggcaaag
     1021 agaccgcaga gtacatcatc aaccactcga agatcctcaa ggtcgggaac acctacatcg
     1081 gtgtgacggg aagcgccacg ttcaagacca tcctgcagga ctatttcggc cgctcgcgca
     1141 cctacgcccg attcgacacg ccgttcgcga tcttcaagac ctggcacaag cttcatgccg
     1201 cactcaagca ggaatactat ctcgtcaccg ggcacagcga ggaagacgtg cttgaatcga
     1261 gccgcatgga cgcgctcatc gtcaacgcac gcggcatctt cggcgtcgcc gcgcaccgca
     1321 ccgtgcagga gttttcgaag ttctacgcgt tcggcagcgg cggcgacttc gcaatgggct
     1381 cgatgcattc cacctacgcc gaggccggcc ggtccgcgga aagcgtcgcg cgcgcggcga
     1441 tcgaggccgc ggccgaattc gacgacagca ccggcgtgcc ggtggactgc tttaccgtaa
     1501 aattggcgcg ccggtaatca caggaggcga agcatgccgt ccttcgacat cgtttccgaa
     1561 gtcaatcagg tcgaggtgca gaacgccgtc gatcagtgca acaaggagat cgccaaccgt
     1621 ttcgatttca aggggtcgga cgcgcgcgtg gaaatcaacg agaaagaaaa gaagctctcg
     1681 gcctacgccg acgacgactt caagctcgga cagcttcggg acgtgcttac gggaaagctc
     1741 gcgaagcgcg gggtcgacgt gcgctgcctg gagctcgcca agatcgaaaa agtgagcggc
     1801 aacagggtga agcaggacat cacggtgcgc gtcggggtcg atcaggacct ggggaagaaa
     1861 atcgtcaagc tcatcaagga cagcaagctc aaggtccagt cgagcattca gggggacgcc
     1921 gtgcgcgtct ccgggtccaa gaaggacgtg ctgcaggaaa cgattgcgct cgtgcgcaag
     1981 tccatcaccg atttcccgct tcagttcaag aattttcgcg agtgaccgcg tctaacgacg
     2041 tgcaccgttc gtgcggttca tcgaagggcg gcgatcagtc ggcgcggagg cgagtgatcc
     2101 agcgctcgta gagccggtct gcgatcgcgt gcagggccgg cagtcgcgcc gggagccgcg
     2161 atgtcatgtc tgctggcgtt tgcacactcg ggacgcgggc ggcgagcgcc tccacttcct
     2221 tttcccagtc cttgcaccaa gtgacgatca tctccttcgt catctcgacg tggcactgca
     2281 tggcgagatg aggcccgagc acgaacatct ggttctcgca gtacgcgctc gaagcaagcc
     2341 gagtcgcccc gggcgggacc gaaaacgctt cgccgtgcca gtgaaaggca ttgaaagccg
     2401 taactgatcc gccgaaccac tccgcagcca gcgcacccgg gagcacctcg actgcgcccc
     2461 acccgatctc ctttacgcgg ttgcgcgcga ccgccccgcc catcgccttc gccataagct
     2521 gtccgccaag gcagtgaccg atcacgggag tcgcttcagc caccgcccgg cgaatcagtg
     2581 cgagagctg
//
LOCUS       DASURG010000557         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1013604, whole genome shotgun sequence.
ACCESSION   DASURG010000557 DASURG010000000
VERSION     DASURG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013604"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1062
                     /locus_tag="VFY80_10055"
     CDS             109..1062
                     /locus_tag="VFY80_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6008298.1"
                     /translation="MGIEKRKVGRAALEVSAIGLGCNNFGLRIDLEQTRAVVDRALAL
                     GVTLFDTADRYGNPPGTSETYLGQVLGERRQSIVLASKFGNAMDDSGRMQGASRRYIK
                     IAVEASLQRLKTDWIDLYQMHRPDPATPIEETLRALEDLVREGKIRYAGCSQFSAEQI
                     REACATAKSAGVTGLVSAQNEYNLLERRAEREFVPALRDCGIGFIPFYPLAGGLLSGK
                     YKDRVVPEGTRYSKPRAQEHRMLNDERWRTLDALHRFCDSRGKSMLEVAVNWLLARPG
                     VASVIAGATRPEQIEQNVNAAGWKPSEGDLAELDRITLPPS"
     gene            complement(1084..2169)
                     /gene="arsB"
                     /locus_tag="VFY80_10060"
     CDS             complement(1084..2169)
                     /gene="arsB"
                     /locus_tag="VFY80_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX6008299.1"
                     /translation="MSAQYELAAGATPRSAISFFERYLTVWVALCIVVGIVLGQVLPQ
                     AFEAISRMEVARVNLPVGLLIWVMIVPMLVKIDFGALHQVTSHWRGIGVTLFVNWAVK
                     PFSMALLGWLFIRHVFAPYLPAEQLDSYIAGLILLAAAPCTAMVFVWSRLSHGDPYFT
                     LSQVALNDTIMIFAFAPIVGLLLGIASITVPWDTLITSVVLYIVIPVVLAQAWRRLLL
                     RRGQAAFEATVSVMGRWSIGALLVTLVLLFAFQGEAIIDQPFVIAMLAVPILLQVFFN
                     STLAYWLNRVVGEKHSVACPSALIGASNFFELAVAAAISLFGLNSGAALATVVGVLIE
                     VPVMLLVVKVVNSTRDWYEGQNTRSRR"
     gene            complement(2201..2668)
                     /locus_tag="VFY80_10065"
     CDS             complement(2201..2668)
                     /locus_tag="VFY80_10065"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX6008300.1"
                     /translation="MKRFHVHVAVEDLDASIRFYAKIFGAEPTVRKPDYAKWMIEDPR
                     VNFAISQRGAKPGIDHLGFQVDTDEELATLRRQVAEAGIAAQDQPNATCCYARSDKYW
                     MTDPQGIAWETYHTLDDAQIYGADAAHSQHIAACCAPRSESVEAGGRMAEKCC"
     gene            complement(2665..3039)
                     /locus_tag="VFY80_10070"
     CDS             complement(2665..3039)
                     /locus_tag="VFY80_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6008301.1"
                     /translation="MDTKTAVTALAALAQDTRLSVFRLLVEAGHEGLPAGTIAETLSL
                     PGSTLSFHLKELGHAGLIVARQEGRSIIYAADFGRMAALMSFLTQNCCRGMPEECVSV
                     MEIALNQCCKPKSQRKVVRSRS"
     gene            complement(3225..>3413)
                     /locus_tag="VFY80_10075"
     CDS             complement(3225..>3413)
                     /locus_tag="VFY80_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008302.1"
                     /translation="AMSDLQDRLVVLSGRSNREAWLQLQRYSDHPNFDQLLRHLERVE
                     GALREQDIEHAVRLYVRT"
BASE COUNT          690 a         1053 c         1060 g          610 t
ORIGIN      
        1 ttcgtcattc cggcgtacgc cggaatccag gggagactgg atcctggctt acgcgggaag
       61 acgaggaacc tcgcatcaat cacgtgacct atgggacacg acatcagaat gggcatcgaa
      121 aaacgaaagg tcggccgcgc ggcactggaa gtctccgcaa tcgggctggg ctgcaacaac
      181 tttggcctcc gcatcgatct cgagcagacc cgcgcggtgg tcgatcgtgc gctcgcgctc
      241 ggtgtcactc tgttcgacac cgccgaccgt tacggcaatc cgcctgggac atcggagact
      301 tacctcggac aggtgctcgg tgaacgacgc caaagcatcg tgctggccag caagttcggc
      361 aatgcgatgg acgacagcgg caggatgcag ggcgcctcgc ggcgctacat caagattgcg
      421 gtcgaggcga gtctgcaaag gttgaagacc gactggatcg atctctatca gatgcaccgt
      481 cccgatcccg cgacgccgat agaggagacg ttgcgcgccc tcgaggacct cgtgcgcgag
      541 ggcaagatcc gctacgcggg ctgttcgcag ttttccgcgg agcagatccg ggaagcctgc
      601 gcaacggcca aatcggcagg cgtgactggt ctcgtctccg cgcagaacga gtacaacttg
      661 cttgagagac gcgccgagcg cgaattcgtg cccgcgcttc gcgactgcgg catcggattc
      721 attccgttct atccgcttgc cggcggactg ctgagcggca agtataagga ccgcgtggtg
      781 cccgagggca cgcgctactc gaagccgcgt gcgcaggagc accgcatgct gaacgacgag
      841 cgatggagga ctctggatgc gctccaccga ttctgcgatt caaggggaaa gtcgatgctc
      901 gaagtcgccg tgaactggct gctcgcacgt cccggcgtcg cgagcgtgat cgccggcgcg
      961 acgcggcccg agcagatcga gcagaacgtc aacgctgcgg gatggaagcc gagcgagggc
     1021 gatctcgccg aactcgatcg tattactttg ccgccgtcct gatcgcgcgc aaagctgttt
     1081 ccctcatcgg cgcgaacgag tgttctgccc ctcataccag tcgcgcgtgc tgttgacgac
     1141 cttcaccacg aggagcatca ccgggacctc gatcagcacg cctacgacag tcgcgagcgc
     1201 cgccccggag ttaaggccaa agagactgat ggccgcggcg accgcgagct cgaagaagtt
     1261 cgatgcgccg atgagtgccg aagggcacgc gacgctgtgc ttttcgccca ccacgcggtt
     1321 tagccaatag gcgagtgtcg aattgaaaaa tacttgcagc aggatcggca ccgcgagcat
     1381 cgcgatgacg aatggctgat cgatgatcgc ctcgccctgg aaggcgaaaa gcagcaccag
     1441 cgtcacgagg agcgcgccaa tcgaccagcg gcccatcacg gagacggtcg cttcgaacgc
     1501 ggcctgccca cggcgcagca gaaggcggcg ccacgcctgc gccagcacca cggggatcac
     1561 gatgtagagc acgaccgacg tgatgagggt gtcccacggt accgtaatgg acgcgatgcc
     1621 cagcagcagg cctacgatcg gcgcgaaggc gaagatcatg atcgtatcgt tcagcgccac
     1681 ctgcgagagc gtgaagtagg gatcgccatg gctcaaccgg ctccagacga acaccatcgc
     1741 cgtgcacggt gccgctgcaa gcagaatcag gccggcaatg tagctgtcga gttgctcggc
     1801 cggcaggtag ggcgcaaaga catgacggat gaagagccaa cccaggagcg ccatcgaaaa
     1861 gggcttgaca gcccagttca cgaagagcgt cacgccgata ccgcgccagt gactcgtcac
     1921 ctgatgcagc gccccgaaat cgatcttgac gagcatcgga acgatcatca cccagatcag
     1981 caggccgacg ggcagattca cccgggcgac ctccatgcgg ctgatcgctt cgaatgcctg
     2041 cggcaatacc tggcccagca cgatgccgac cacaatgcac aacgctaccc agaccgtgag
     2101 atagcgctcg aagaacgaga tcgcgcttcg cggcgtggct cctgcggcga gttcgtattg
     2161 cgcactcatg aagatgcttt tccgggactg tgctcgttcg ctagcagcac ttctcggcca
     2221 tccgaccgcc agcttcgacg ctttctgacc ggggagcaca gcaggcggcg atgtgttgcg
     2281 agtgcgcggc gtccgccccg tatatctgcg cgtcatcgag cgtgtggtag gtctcccagg
     2341 cgatgccctg cgggtcggtc atccagtatt tgtccgaccg cgcataacag caggtcgcat
     2401 tgggctgatc ctgagcggcg atgcccgctt cagcgacctg gcggcgcagc gtggccaatt
     2461 cttcgtcagt gtccacctga aagccgaggt gatcgatgcc gggcttcgcg ccgcgttggg
     2521 agatcgcgaa gttgaccctt gggtcctcga tcatccattt tgcgtaatcg ggctttcgca
     2581 ccgtcggctc ggcgccgaaa atcttcgcgt aaaagcggat gctggcatcg aggtcttcta
     2641 ctgcaacatg cacgtgaaag cgtttcatga tcgactcctg acgactttgc gttgcgattt
     2701 aggcttgcag cattggttga gcgcaatctc catcacggac acgcactctt caggcatccc
     2761 ccggcagcag ttctgcgtca ggaagctcat gagagccgcc atgcgtccga aatcggccgc
     2821 gtagatgatg gagcgtcctt cctggcgcgc gactatcagg cccgcgtgtc ccagctcctt
     2881 cagatggaac gacaacgtcg aaccgggcag ggacaaggtc tcggcaatcg ttcctgccgg
     2941 aagtccttcg tggcccgcct caaccagcag ccggaacacg ctcaaccggg tgtcctgtgc
     3001 gagcgccgcc agtgccgtga ctgccgtctt agtatccatg tttccagtat catggaaata
     3061 ttgagccggt gtcaacaccg agggacggac ccggcgttga accggccgtt gcattgaggg
     3121 tctgacaaga gccaccgtgt ggaaccctta cgggtctggt ggtcggggat gtcgcgcgca
     3181 gcaccgtgcg gcatccccgc tttgtattcc tgagtcaggc tgccctacgt tcgaacgtag
     3241 agcctgacgg catgctcgat atcctgttcc cgcagcgcac cttcgacccg ctcgagatgg
     3301 cgcagaagct ggtcgaaatt cgggtgatcg ctgtaacgct ggagttgcag ccacgcctcg
     3361 cgattcgacc ggccgctcag tacgaccaat ctgtcttgaa gatctgacat ggc
//
LOCUS       DASURG010000558         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_707103, whole genome shotgun sequence.
ACCESSION   DASURG010000558 DASURG010000000
VERSION     DASURG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707103"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1669)
                     /gene="tcuA"
                     /locus_tag="VFY80_10080"
     CDS             complement(164..1669)
                     /gene="tcuA"
                     /locus_tag="VFY80_10080"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6008303.1"
                     /translation="MTQQSYDVVVIGGGNAGLCAALSARERGARVLLLERSPADQRGG
                     NTAYTGGGMRMIHHGVETVKSVVPDLSEGEIANTDFGQYTAEEYLEDLGRVTQWYCDP
                     DLAETLVRNSTQTVQWLHGHGVRFVPRFGRYAFRHEGKFKFFGGTVVEAAGGGRGLVQ
                     FEYTAAEKAGIDIRYGTQAVALIRTRVGVTGVRVNVDGVEEDTIAATAVVLAAGGFEA
                     NREWRTRYLGPGWDMAKVRGTRYNTGDGIRIALDIGAQPYGQWSGCHSVSWERYALDF
                     GELDRPITASRNGYPFGIMVNARGKRFVDEGVDFRNYTYAKYGRIVLEQPGSYAWQVF
                     DAQVEHLLHDEYRSKGTTKVQADTLEELVARMEDVEPEQFLKTVQQYNAAITREVPFN
                     PNVKDGRRTVGLEVNKSNWALVLEKPPFVAYAVTCGITFTFGGLKIDTTAQVLDMADR
                     PIPGLYAAGELVGGLFYYNYPGSSGLMAGAVFGRIAGREAAAHALENRCAA"
     gene            1758..>2565
                     /locus_tag="VFY80_10085"
     CDS             1758..>2565
                     /locus_tag="VFY80_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6008304.1"
                     /translation="MNTPTKGKRLRELIHRKEKVLVVLHPPTATHARIMERAGCEVGF
                     VGTGGVVGGYTGLADVGTLTMMECVQIAGWIAQTVNFPIIIDGDTGHGGVMAVRRLVR
                     ECIRAGIAGARIDDQPLEGKRRTQSAGMEVVPLEQAIARYRAAVDMKNELDPEFVIMA
                     QCYARDAWNGGIDDAITRLTAYREQGGVDWVQLESPHSIDEIKRARAAVEGPFSFMKG
                     KLPRYLGLDEHLALGVNIAWYPGFTHHVTWAALWDFMQDFQSRGIEAWEAF"
BASE COUNT          473 a          800 c          806 g          486 t
ORIGIN      
        1 gcacctggag tgccccgcgt tcggagtaca cccgctctgc ctgggcaaaa cggaggtcgg
       61 cgccagcgag cgtggctttg gccaacgtgc aaaacgcttc caccggctcg tgcaccgggc
      121 ccacatcgcc gcatccaagc aaagtgatgg tgccgttcat cgcttacgcg gcgcaccggt
      181 tttcaagggc gtgtgcggcc gcctcgcggc cggcaatgcg cccgaatacc gccccggcca
      241 tgagtcccga gctgccgggg tagttgtagt agaagagacc gccgacgagc tcgcccgccg
      301 catacaggcc cggaatgggc cgatcggcca tgtcgagcac ctgcgcggtc gtgtcgatct
      361 tgagtccgcc gaacgtgaat gtgatgccgc aggtgaccgc atacgcgacg aacggcggct
      421 tttcgagaac cagggcccag ttcgacttgt tgacttcgag tccgaccgtg cgacggccgt
      481 ccttcacatt gggattgaaa ggcacctcgc gcgtgatggc ggcgttgtat tgttgcaccg
      541 tcttcagaaa ctgctccggc tcgacgtcct ccatgcgggc gacaagctcc tcgagcgtgt
      601 ccgcctgaac cttggtggtg cctttcgaac gatactcgtc gtgaagcaga tgctcgacct
      661 gcgcgtcgaa cacttgccac gcgtagctgc ccggctgctc gagcacgatg cgtccgtact
      721 tggcatacgt gtagttgcga aaatccacgc cttcgtcgac gaaccgcttg ccgcgcgcgt
      781 tcaccatgat tccgaacgga tagccgttgc gactcgcggt gatcggacgg tcgagctcgc
      841 cgaaatcgag ggcatagcgc tcccacgaga ccgagtgaca acctgaccat tgaccgtacg
      901 gctgcgcgcc gatatcgagc gcgatccgga tgccgtcgcc tgtgttatag cgcgtgccgc
      961 gcaccttggc catgtcccag cccgggccga gatagcgcgt gcgccattca cggttcgcct
     1021 cgaaaccgcc tgcggcgagc accacggcag tggcggcaat ggtgtcttct tcgacaccgt
     1081 cgacattgac gcgcacgccg gtcacgccaa cgcgggtacg gatcaacgcg acggcctgcg
     1141 tgccatagcg gatgtcgatg ccggcttttt ccgcggccgt gtattcgaat tgcacgagcc
     1201 ctcggccacc gccggccgct tctacgaccg tgcccccaaa aaacttgaac ttgccttcgt
     1261 gcctgaaggc gtagcggccg aagcgtggca cgaaccgcac gccgtggccg tgcagccatt
     1321 gcacggtctg cgtgctgttg cgcacaagcg tttcggcgag atccgggtcg caataccact
     1381 gtgtcacgcg cccgagatct tcgagatatt cctccgcggt gtactgaccg aagtcggtgt
     1441 ttgcgatctc gccttcggac agatccggca cgacgctctt caccgtctcc actccgtgat
     1501 gaatcatgcg catgccgccc ccggtgtagg cggtgttgcc gccacgctga tctgcgggcg
     1561 agcgctccag aagcaggacg cgggcgccac gctcgcgtgc cgaaagcgca gcacacaagc
     1621 ctgcgttacc gccgccgatc accactacgt cgtaactctg ctgggtcacg tcttcacttt
     1681 ttagagcata cgtccgcatg catatgtata catgagaata cgttatactc gccgaggccg
     1741 tcaacgaaac tctgtttatg aacacgccaa ccaaaggcaa acgactgcgc gagctcatcc
     1801 accgtaagga gaaagtgctg gtggtgctcc acccgcccac tgcgactcac gcacgcatca
     1861 tggagcgcgc cggatgcgaa gtgggtttcg tcggtaccgg tggcgtggtc ggcgggtata
     1921 cggggcttgc cgacgtgggc accctcacga tgatggaatg cgttcagatc gccggctgga
     1981 ttgcgcagac agtcaacttc ccgatcatca ttgacggcga caccggtcat ggcggggtga
     2041 tggcggtgcg acgtctcgtg cgtgaatgca tccgcgctgg gatcgcgggg gcccgcatcg
     2101 acgaccaacc gctcgaaggc aagcggcgca cgcagagcgc ggggatggaa gtcgtgccgc
     2161 tggagcaggc gatcgcgcga taccgcgccg cggtagacat gaagaatgag ctcgatcccg
     2221 agttcgtcat catggcgcag tgctacgcgc gtgacgcgtg gaatggcgga atcgatgatg
     2281 cgatcacacg gctcacggcc tatcgggagc aaggaggggt cgactgggtg cagctcgagt
     2341 cgccgcattc gattgatgag atcaagcggg cgcgtgcagc ggtcgagggt cccttctcgt
     2401 tcatgaaagg taaactgcct cgttacctcg gattggatga gcatctcgcg ctaggcgtta
     2461 atatcgcttg gtatcccggc tttacgcacc acgtgacctg ggcggcgctg tgggatttca
     2521 tgcaggattt tcagagccgc gggatagaag cttgggaagc cttcg
//
LOCUS       DASURG010000559         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_196543, whole genome shotgun sequence.
ACCESSION   DASURG010000559 DASURG010000000
VERSION     DASURG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196543"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFY80_10090"
     CDS             complement(<1..623)
                     /locus_tag="VFY80_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6008305.1"
                     /translation="MGNLVRCAVAAIALSLFAAGVQAQSKYPTRPIRLLTPFAPGGGS
                     DLLARLIGPQLTEVLGQPIVVDNRPGGGGTLGAGIAARAEPDGYTLILVSGSYGANEA
                     THNLPFDTVKGITPIILIGTTPLVLTIHPKTPIKSIKEFIDYAKANPGKLNIASAGVG
                     GLDHLAGELLKLQTGINYTTVPYKGSGPAMTALVSGQVNGSMTTLVT"
     gene            complement(710..1153)
                     /locus_tag="VFY80_10095"
     CDS             complement(710..1153)
                     /locus_tag="VFY80_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6008306.1"
                     /translation="MPSTVRLHRVLRATPERVYRAFLDPDAMAKWLPPYGFTCKVHQL
                     DAKAGGTYRMSFTNFHTEKGHAFGGRYLEMKPNELIRYTDKFEDPNLPGEMLTTVTLK
                     KVSCGTELNIVQEGLPDVIPAEACYLGWQESLAQLAHLVDPEIPD"
     gene            complement(1369..2583)
                     /locus_tag="VFY80_10100"
     CDS             complement(1369..2583)
                     /locus_tag="VFY80_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6008307.1"
                     /translation="MLDIRLSQEQREFRDLARKFAQEEIKPKAMALDREPEWEKRIPW
                     ELLKKGSQLGFRTFVLSEENGGSGLSDHVTSCLVAEELAAGEIGTAYYFMLTARRARD
                     WFELRMTPEQRAYFLPKFLDDDLYFTTVAIHEPETDFGFDYFTETPDNVRLVTRAVEQ
                     PDGSWIINGAKAYQTVGYLAKLIVVRAQTPAGVQSFLVEGDSHGLTRRPLSKLGRRVG
                     DNAAEMFFDNVRVPKGRIMPPAPKGRTDMGTHPTIAALTLGLGRAAFEETLKYAQERV
                     SGGKHIIKHQAVGIAIADMAMNLEAARRLIWTAAWVKDHPEAIEDGSVENLPYEHYAT
                     AFTGAAVQRLTEQALELFGGAGVIQGMPIEKYVRDAHVQKHVSFPYPTRFKITEALVG
                     FRRKIPPFHGGD"
BASE COUNT          450 a          815 c          833 g          530 t
ORIGIN      
        1 ctggtcacga gcgtcgtcat gctgccgttg acctggcccg agaccaacgc cgtcatggcg
       61 ggaccgctgc ctttatacgg gacagtggtg taattgatgc cggtttgcag cttgagcagt
      121 tcgcccgcca ggtgatcgag cccgcccaca cccgccgatg cgatattgag ctttccggga
      181 ttcgccttgg cgtaatcgat gaattccttg atgctcttga tcggcgtctt gggatgtatc
      241 gtgagcacga gcggcgtggt cccgatgagg atgatcggcg taataccctt taccgtatcg
      301 aacggcaggt tgtgcgtcgc ttcgttggcg ccgtagcttc cggaaacgag gatgagcgtg
      361 tagccgtccg gctcggcgcg cgccgcgatg cctgcgccga gcgtgccgcc gccgcccggc
      421 cggttgtcca cgacgatggg ctgcccgagc acctcggtca actgcgggcc gatcagccgc
      481 gcgagcaagt ctgatccgcc gcccggcgca aaaggggtca ggagccggat cggtcgggtc
      541 ggatacttgg attgagcctg aacgcctgca gcaaagagcg agagcgcaat tgcagcgacg
      601 gcgcaacgca cgagattgcc catgagtctt cctcccacac ggaaatcccg gtcagtgtaa
      661 cgtgtccgcc tcggcgttgc tacgcgcgcc gctaaccctc cctctttttt cagtcgggga
      721 tttcgggatc gacgaggtgt gcgagctgcg cgagcgattc ctgccatccg aggtagcacg
      781 cctccgcggg aatgacatcg ggcagccctt cctgcacgat gttgagctcc gtcccgcagg
      841 acactttctt gagagtgacg gttgtcagca tttcgcccgg cagattggga tcttcgaatt
      901 tgtcggtata gcggatgagt tcgttcggct tcatctcgag atagcgcccg ccgaaggcgt
      961 ggcccttctc cgtgtggaaa ttggtgaacg acattctgta cgtgccgcct gccttggcat
     1021 cgagctgatg aaccttgcag gtgaagccgt aaggcggaag ccacttggcc atggcatccg
     1081 ggtcgagaaa cgcccgatag acgcgctcgg gcgtggcgcg aagtacgcgg tgaaggcgga
     1141 cggtgcttgg catggttcgc tcctttcgtt gtgatcatcc cattgtcgat tgaacctgtg
     1201 agaaatcgac atgtgcggag ttcgcccgga ggccggcctc cggacggctc atgtccggag
     1261 gctcgttctc tggacaccgg ctttcgccgg tgtgacgaaa cgtgccggtg tgacgcgata
     1321 tgccggcgtg acgaaatcgg tgcgacgagt gaatagccag tgcgggggct aatcgccgcc
     1381 gtggaagggt ggaatcttgc gcctgaagcc gacgagcgcc tcggtgatct tgaaccgcgt
     1441 gggatacgga aacgagacgt gcttctgcac gtgcgcgtcg cgcacatact tctcaatcgg
     1501 catgccttgg atcacgcccg ccccgccgaa aagctcgagc gcctgctcgg taagccgctg
     1561 aacggccgcg cccgtgaacg cggttgcgta gtgctcgtag ggcagattct ccacgctgcc
     1621 gtcctcgatc gcttcgggat gatccttgac ccacgcagcg gtccagatga ggcgccgcgc
     1681 cgcttccaga ttcatcgcca tgtcggcgat cgcgatgccc accgcctgat gcttgatgat
     1741 gtgcttgccg cccgagacgc gttcctgtgc gtacttgagt gtctcctcaa aggcggcacg
     1801 tcccaatccc agcgtaagcg cggcgatggt cggatgcgtg cccatgtcgg tgcggccctt
     1861 cggcgcgggc ggcatgatgc gtcccttcgg tacccgcacg ttgtcgaaga acatctcggc
     1921 cgcgttgtcg cccacgcgcc gcccgagctt agagagcgga cgcctggtca gcccatgtga
     1981 atcgccctcg accagaaacg actgcacgcc cgctggtgtc tgcgcccgca cgacgatcag
     2041 cttcgcgagg tagccgacgg tctggtaggc cttcgcgccg ttgatgatcc atgagccgtc
     2101 cggctgctcg accgcgcgtg tgacgagccg cacgttgtcc ggcgtttcgg tgaagtagtc
     2161 gaaaccgaag tctgtctccg gctcgtgtat cgcaaccgtt gtgaaataga gatcatcgtc
     2221 gaggaacttc ggcaggaaat aggcccgctg ctccggcgtc atgcgcagtt caaaccagtc
     2281 gcgcgcgcgt cgcgccgtca gcatgaagta atacgcggta ccgatctcac ccgccgcgag
     2341 ctcctccgcg acgagacacg acgtgacgtg atccgacagg ccggagccgc cgttctcttc
     2401 cgacagcaca aaggtccgga agccgagttg cgagcctttc ttgagcagct cccacggaat
     2461 gcgcttctcc cactcgggct cgcgatcgag ggccatggcc ttgggcttga tctcttcctg
     2521 cgcgaacttg cgcgccaggt cgcgaaattc gcgctgctcc tgcgaaagac ggatatccag
     2581 catggctctc ctctatggcc tcaagacgat cttccccatg gtgcgccg
//
LOCUS       DASURG010000560         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1525860, whole genome shotgun sequence.
ACCESSION   DASURG010000560 DASURG010000000
VERSION     DASURG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525860"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFY80_10105"
     CDS             complement(<1..858)
                     /locus_tag="VFY80_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX6008308.1"
                     /translation="MARITVDPITRIEGHLRIDVEVDGGAVRNSWSSGQMWRGIELIL
                     KGRDPRDAWLFTQRICGVCTTVHAIASVRAVEDALDLEIPLNAQYIRNLVLIAHALHD
                     HIVHFYHLSALDWVDVTGVLKADPKKTTALAESLSPWTNNGVKEMTAVKERVATLVSG
                     GQLGIFTNGYWGHPAMKLSPEVNLLAVSHYLQALEYQRKANKIVTMLGGKTPNIQNLA
                     VGGVANPINLDNQSALNMEKLYHIKQLFTEVDAFIKQVYLPDVTAIGAMYPDWLGYGA
                     GVTNYLAVPD"
     gene            complement(876..2123)
                     /gene="hybB"
                     /locus_tag="VFY80_10110"
     CDS             complement(876..2123)
                     /gene="hybB"
                     /locus_tag="VFY80_10110"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEX6008309.1"
                     /translation="MTHVAAPVGGRILTKPMKVLLAIVAIAVVMLIWRFAVGLRMTAM
                     SDRYPWGIWIAYDVVTGTALACGGYAVAILVYILNKGRYHPLVRSAILTSALGYTLGG
                     MSVVIDLGRWWNVWQVPILFWDWNLNSILLEVALCIMLYTLVLWIELSPAFLESWKKS
                     GNTRLSNFANATLPPLEKALPWIIALGILLPTMHQSSLGSLMMLAGQKVHPLWQTPLL
                     PLLFLISVVAMGYGAVVIESTLSSRFFKRPDESKILYRLARVVAPITLVYVGLRVIDL
                     VLRGKAGLIFTSGGFSLLFLIEMALFIAPVVMVYGKTAKRAGTLFRVAMLVLLAGALY
                     RFSTFLVAFRPAEGWVYFPSIVEILVTMGVIAGEIAAYIFIVKKFPILSAPAPARPKV
                     RPVPESVLKNIGISPAASPGGAD"
     gene            complement(2120..2998)
                     /gene="hybA"
                     /locus_tag="VFY80_10115"
     CDS             complement(2120..2998)
                     /gene="hybA"
                     /locus_tag="VFY80_10115"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008134.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HEX6008310.1"
                     /translation="MDRRDFIRGVLAAGTVTAAAANAKPMRHREPAPDAVGMLYDSTL
                     CTGCKACVVACRKENGFEPETKDGLHDMQTELNGNTKNVIKLYQEPNDKSVFAFVKQQ
                     CMHCADPGCVSVCMLGALHKGEKGIVEYNPDRCIGCRYCQIGCPFNVPAFEWQSNTPQ
                     IVKCELCKERLAEGKEPACVEVCPRDAVIFGNVNDLLKDAHARIEAEPDRYENKVYGE
                     TDGGGTQVLYLTSKQVSFASLGLPDLGEKSVPSLPEKIQHTIYKGFFAPVALYAVLGA
                     VVFRNRKRGQEEEVES"
     gene            complement(3001..>3492)
                     /locus_tag="VFY80_10120"
     CDS             complement(3001..>3492)
                     /locus_tag="VFY80_10120"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466348.1"
                     /note="involved in hydrogen uptake; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HEX6008311.1"
                     /translation="LPALDDKGRPLFAYGRTIHEHCPRRAHFDAGRFAEKFGDENHRQ
                     GYCLYKLGCKGPSTYANCSLQHFAEVDGAWPIGVGHPCVGCTEQSLLFRAPIHDTVDI
                     ARATPPDSYPPIEVQHAGVDPVATGVGGAVLGGIIGAASLGAMKISKSSKTTPTEPPN
                     KEG"
BASE COUNT          660 a         1106 c         1089 g          637 t
ORIGIN      
        1 gtccggcacc gcgaggtagt tggtgacgcc cgcgccgtag cccagccagt ccggatacat
       61 cgcgccgatc gcagtgacat ccggcagata gacctgcttg atgaaggcgt cgacttccgt
      121 gaagagctgc ttgatgtggt acagcttctc catgttcagc gcggactggt tgtccaggtt
      181 gatgggattg gcgacaccgc ccacggccag gttctggatg ttgggcgtct tgccgcccag
      241 catggtcacg atcttgttgg ctttgcgctg atactcgagc gcctgcaggt aatgcgagac
      301 cgccagcagg ttcacttccg gcgagagctt catggccggg tgaccccagt agccgttggt
      361 gaagatgccg agctggccgc cgctgaccag cgtggccacg cgctccttca ccgcggtcat
      421 ttccttcacg ccgttgttcg tccacggcga cagactttcg gccagtgccg tcgtcttctt
      481 cgggtccgcc ttgagcacac ccgtcacatc cacccagtcg agtgccgaca ggtgatagaa
      541 atgcacgatg tgatcgtgca gcgcgtgtgc gatcaacacc aggtttctga tgtattgcgc
      601 attgagcggg atctccaggt cgagtgcgtc ctctacggca cgcactgatg cgatcgcatg
      661 cacggtcgtg cacacgccgc aaatgcgctg cgtgaacagc catgcgtcac gagggtcgcg
      721 tcccttcaga atcagctcga tgccgcgcca catctggccc gacgaccagg agttccgcac
      781 ggcgccgcca tccacctcga cgtcgatacg cagatggcct tcaatgcggg taatgggatc
      841 aacagtgata cgtgccatgt tctctcctaa gtcgatcagt cagcgccgcc gggcgacgcg
      901 gcgggtgaaa taccaatgtt ctttaatacg ctttcgggaa cgggccgcac cttcggtcgt
      961 gcgggcgcgg gtgcactcag gatcgggaat ttcttcacga tgaagatgta cgccgcgatt
     1021 tcacccgcga ttacgcccat ggtaaccagg atctcgacta tggacgggaa gtagacccaa
     1081 ccctccgccg gccggaacgc caccaggaac gtggagaagc ggtagagtgc gcccgcgagc
     1141 agaaccagca tcgcgacgcg gaacagtgtt ccggcgcgct tggcggtctt gccgtaaacc
     1201 atcaccacgg gcgcaatgaa gagcgccatc tcgatgagga acagcagact gaacccaccc
     1261 gatgtaaaga tcagcccggc cttgccgcgc aggaccaggt cgatcacgcg caagccgaca
     1321 tacaccagcg tgatgggcgc aaccacgcgc gccaggcgat acagaatttt gctttcatcc
     1381 ggacgcttga agaaacggct ggacagcgtc gattcaatga cgaccgcgcc gtatcccatg
     1441 gcgaccacgc taatcaggaa aaggagcggc aacagcggcg tttgccacaa cggatgcacc
     1501 ttttggccag ccagcatcat caggctgccg agggaggact ggtgcatggt gggcaggaga
     1561 atgccgagtg cgatgatcca gggcagcgcc ttctccagcg gcggcagtgt cgcattggcg
     1621 aagttgctca acctggtgtt gccgctcttc ttccacgatt ccaggaacgc cggtgacagc
     1681 tcgatccaca gcaccagcgt atacagcatg atgcagagcg cgacttccag cagaatggaa
     1741 ttgagattcc agtcccagaa caggattggc acctgccaga cgttccacca gcggccgaga
     1801 tcgatgacca cgctcatgcc gcccagcgtg taaccgagcg cgctggtcag aatcgcagag
     1861 cgcaccagcg gatggtagcg tcccttattc aggatgtaga cgagaatcgc gacggcgtaa
     1921 ccgccgcacg cgagcgcggt gccggtcacg acgtcgtacg cgatccagat cccccaggga
     1981 tagcgatcgc tcatggccgt catgcgcaga ccaaccgcga aacgccagat cagcatgacc
     2041 accgcgatcg ccacgatggc caacagcact ttcatcggct tggtcagaat gcgaccgccg
     2101 acgggagccg cgacgtgggt catgactcca cctcctcttc ctgaccgcgc ttgcggttgc
     2161 ggaataccac cgcgcccagc accgcataca gcgcgaccgg ggcgaagaag cctttgtaaa
     2221 tggtgtgctg aatcttctcg ggcagggacg gcaccgactt ctcaccgagg tcgggcaagc
     2281 cgagactggc gaaggacacc tgcttcgacg tcagatagag cacctgcgtg ccgccgccat
     2341 cggtttcgcc gtacaccttg ttctcatacc tgtccggctc tgcttcgatg cgtgcgtgcg
     2401 catccttcaa caaatcgttg acgttgccga aaatgaccgc gtcccgcggg cagacctcca
     2461 cgcaggccgg ctccttgcct tcagcgagtc gctccttgca cagctcgcac ttgacgatct
     2521 gtggtgtgtt cgattgccac tcgaaggcag gcacgttgaa gggacagccg atctggcaat
     2581 aacggcagcc gatgcagcgg tccggattgt attcgacgat gcccttctca cccttgtgca
     2641 gcgcgcccag catgcagacg ctgacgcacc ccgggtccgc gcagtgcatg cactgctgct
     2701 tcacgaacgc gaacacagac ttgtcgttcg gctcctggta gagcttgatg acgttcttgg
     2761 tgttcccgtt gagctcggtc tgcatgtcgt gcaggccgtc cttcgtctca ggctcgaagc
     2821 cgttctcctt gcggcaggcg acgacgcacg ccttgcaacc ggtgcagagc gtggagtcgt
     2881 agagcatgcc caccgcgtcg ggcgccggct cgcgatgccg catgggcttc gcgttggcgg
     2941 cggccgccgt cacggttccc gcggcgagca cccctcggat gaagtctcgg cggtccatgg
     3001 ctacccttcc ttgttcgggg gctccgtggg cgtggtcttc gacgacttcg agatcttcat
     3061 cgcgccgagc gaagcggcgc cgatgatgcc gccgagcacc gcgccgccca cgccggtggc
     3121 gaccgggtcg acgcccgcgt gctgcacctc gatgggcggg tacgaatccg gcggcgtcgc
     3181 gcgggcgatg tcgaccgtgt cgtggatggg cgcgcggaag agcagcgact gctccgtgca
     3241 gcccacgcag gggtggccga cgccgatggg ccaggcgccg tcgacctcag cgaagtgctg
     3301 gagcgagcag ttcgcgtagg tcgagggacc cttgcagccg agcttgtaga ggcagtagcc
     3361 ctgacggtga ttctcgtcgc cgaacttctc ggcgaagcgg ccggcatcga agtgcgcccg
     3421 gcgcgggcag tgctcatgga tcgtgcgacc atacgcgaac agcggacgac ccttgtcgtc
     3481 gagcgcgggc ag
//
LOCUS       DASURG010000561         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_707578, whole genome shotgun sequence.
ACCESSION   DASURG010000561 DASURG010000000
VERSION     DASURG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707578"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFY80_10125"
     CDS             <1..567
                     /locus_tag="VFY80_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX6008312.1"
                     /translation="KGQSPEPFDLLYWNADSTNLPGAMYVYYIRNMYLENNLRKPGRL
                     TMCGVPVDLGKLDMPAYVLATREDHIVPWKTAHASARLLRGTTDFVLGASGHVAGVVN
                     PAWKNRRSYWSNSTLEDDADRWLANAETHPGSWWTHWSEWLKQHGGDNVPAREKLGGN
                     GGTEVEPAPGRYVREKAHPPRAAAGTAR"
     gene            604..1344
                     /gene="phbB"
                     /locus_tag="VFY80_10130"
     CDS             604..1344
                     /gene="phbB"
                     /locus_tag="VFY80_10130"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX6008313.1"
                     /translation="MARVTVVTGGMGGLGEAICTKLAKIGYRVVATYSPGNKKWGDWL
                     AEMKRQGYDFHAAEVDVADYESCQRAVATIQAEVGLIDVLVNNAGITRDTTFRKMEKA
                     AWDQVLRTNLDSVFNMTKPVIEGMAERGWGRIINVSSVNGQKGAFGQTNYSAAKAGMH
                     GFTKALALEYARKGVTVNTISPGYIGTKMVLAIPQEILESKIIPQIPIGRLGKPEEVA
                     GLVAYLCTEEAAFVTGANIAINGGQHMY"
     gene            1488..2093
                     /gene="phaR"
                     /locus_tag="VFY80_10135"
     CDS             1488..2093
                     /gene="phaR"
                     /locus_tag="VFY80_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX6008314.1"
                     /translation="MSAQLRIIKKYPNRRLYDTETSSYITLADVKKLVLENIEFRVED
                     AKSKEDLTRSILLQIILEEETAGAPMFSCDMLSQIIRFYGNAMQGMMGNYLEKNIQTF
                     IEIQKRLQDQSRQVYGQNPMLNSETWSEFVKMQGPAIQGLMGHYLEQSANAFLEMQQQ
                     LQQQTRNLFTNFPFPNFGTTGQKPESPSGGTDKGDKGEGGR"
     gene            2129..>2731
                     /locus_tag="VFY80_10140"
     CDS             2129..>2731
                     /locus_tag="VFY80_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6008315.1"
                     /translation="MRRRIPKVGFVSLGCPKALVDSERILTQLRAEGYAIAPRYSNAD
                     LVIVNTCGFIDAAIDESLEAIGEALAENGKVIVTGCLGAQGDLVRKTHPRVLAVTGPH
                     ATEEVMQAVHTHLPQLHDPYTSLIPPQGIRLTPQHYAYLKISEGCNHRCTFCIIPSMR
                     GDLVSRPVGEVLQEAENLVRAGVKELLVISQDTSAYGVEMK"
BASE COUNT          603 a          815 c          845 g          468 t
ORIGIN      
        1 aagggacagt cgcccgaacc cttcgatctc ctctactgga atgccgacag cacgaacctg
       61 ccgggggcaa tgtacgtcta ttacatacgc aacatgtatc tcgagaacaa tctgcgcaaa
      121 ccgggccgcc tgacgatgtg cggtgtacca gtggatctcg gcaagctcga catgccggcg
      181 tatgtgctcg ccacccgcga agatcacatc gtgccgtgga aaaccgcgca cgcgagcgcg
      241 cggctgctgc gcggcacaac ggatttcgtg ctgggcgcga gcggacacgt ggcaggcgtg
      301 gtgaatccgg cgtggaagaa ccgccgcagc tattggtcca acagcacgct cgaagacgac
      361 gcggaccggt ggcttgcgaa tgccgaaaca catccgggca gctggtggac gcactggagt
      421 gaatggctga agcagcacgg cggcgacaat gtcccggcac gggaaaagct cggcggcaac
      481 ggcggtaccg aggtcgagcc tgcgcccggg cgctacgtca gggaaaaagc acacccgcca
      541 cgcgcggcgg cgggcaccgc gcgataggcg acaacgagcg ttttttctgc gaggagaatc
      601 aacatggcac gagtgacggt ggtgaccggc ggtatgggcg gactcggcga ggcaatctgc
      661 accaagctcg ccaagatcgg ctatcgggtc gtggcaacct attcgccggg caacaagaaa
      721 tggggcgact ggctcgccga aatgaagagg cagggatacg acttccatgc cgccgaagtc
      781 gatgtggcgg attacgagtc gtgccagcgc gcagtggcca cgatccaggc ggaggtcggc
      841 ctgatcgacg tactcgtcaa caatgccgga atcacgcggg atactacttt ccgcaagatg
      901 gaaaaggccg cttgggacca ggtgctccgc accaatctcg acagcgtctt caacatgacc
      961 aagcccgtga tcgagggcat ggccgagcgc ggctggggcc gcatcatcaa cgtgtcctcg
     1021 gtgaacggac agaagggcgc cttcggacag accaactact ccgcggcaaa agccggcatg
     1081 cacgggttca cgaaggcgct cgccctcgag tacgcgcgca agggggtgac cgtcaacacg
     1141 atttcccccg ggtacatcgg gaccaagatg gtgttggcga taccgcagga aatcctggaa
     1201 tcgaagatta ttccgcagat cccgatcggg cgactgggca agcctgagga agtggcgggt
     1261 ctggtcgctt atctctgcac cgaggaagcc gctttcgtga ccggggccaa tatcgcgata
     1321 aacggcggcc agcatatgta ctgacgctgc ctgcgacggc cgatggcact gcaacacgct
     1381 gcgcacgagc gttatgaatc tgatttagct gagaatttct gggttctcgg tttgcaatgc
     1441 cgcaccatct ctgaaataat cggtcattcg catagcgggg gccttccatg tcagcacaac
     1501 tcagaattat taaaaagtac cccaatcgcc ggctttacga tacggaaaca agcagttaca
     1561 tcacactcgc ggacgtcaag aagctggtgc tcgagaatat tgaattccgg gtcgaggatg
     1621 cgaagagcaa ggaggacttg acgcgcagca ttctgctgca gatcattctc gaagaggaaa
     1681 ccgcgggcgc cccgatgttt tcatgcgaca tgctctcgca gatcattcgc ttctacggga
     1741 atgcgatgca gggcatgatg ggcaactacc tggaaaaaaa catccagacc ttcatcgaga
     1801 tccagaagcg cctgcaggat cagtcgcgcc aggtgtacgg ccagaacccg atgctcaact
     1861 ccgagacgtg gagcgagttc gtcaagatgc agggtcccgc gatccagggg ctgatgggcc
     1921 actacctgga gcaaagcgcg aacgcctttc tcgaaatgca gcagcagctg cagcagcaga
     1981 cccgtaatct cttcaccaat tttcccttcc ccaacttcgg gactaccggg cagaaaccgg
     2041 aatcgccttc gggcggtaca gacaagggcg acaaaggcga aggcggccgt tgagtcggac
     2101 tgcggttgca ggtcattccg tccggccggt gcgccggcgc atccccaaag tggggtttgt
     2161 atcgctcggc tgtcccaagg cgctcgtcga ttccgagcgc atcctcacgc agcttcgcgc
     2221 cgaagggtat gcgattgcgc cgcgctattc caatgccgac ctcgtcatcg tcaacacctg
     2281 cggcttcatc gacgcggcga tcgacgagtc gctggaggcc atcggcgagg cgctcgccga
     2341 aaacggcaag gtgatcgtca cgggttgtct gggcgcgcag ggcgacctcg tgcgcaagac
     2401 tcacccgcgc gtcctggccg tcaccggtcc gcacgccacc gaagaggtca tgcaggcggt
     2461 ccacactcac ctgccccagc tgcacgatcc gtatacgagc ctgattccgc cgcaaggcat
     2521 caggcttacg ccccaacatt acgcctatct caagatttcg gaaggctgca atcaccgttg
     2581 caccttctgc atcattcctt ccatgcgcgg cgacctcgtg agccggccgg tcggggaggt
     2641 gctgcaggag gccgagaacc tcgtgcgcgc cggggtgaag gagctgctcg tcatctccca
     2701 ggacacgagc gcgtatggcg tcgaaatgaa a
//
LOCUS       DASURG010000562         5542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_94746, whole genome shotgun sequence.
ACCESSION   DASURG010000562 DASURG010000000
VERSION     DASURG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94746"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VFY80_10145"
     CDS             <1..142
                     /locus_tag="VFY80_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796483.1"
                     /note="catalyzes the transfer of electrons from NADPH to
                     thioredoxin; FAD/NAD(P) binding; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX6008316.1"
                     /translation="TATSVPGVFAAGDVQDHVYRQAVTSAGSGCMAALDAQKYLEGKG
                     LV"
     gene            173..724
                     /locus_tag="VFY80_10150"
     CDS             173..724
                     /locus_tag="VFY80_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX6008317.1"
                     /translation="MKKDRSKPMAEELEALRALLPDVTPIPRANRASVAPPPPAPVAL
                     QRLRDERAALADSLNPRDPWESGLETGDELSFLRPGLAPITLRKLRRGHWVIQDEIDL
                     HGLTTPQAHELLAGFLGASLRRGLRCVRVIHGKGLRSKNREPVLKNKVARWLMQRQEV
                     LAYCQARRTEGGGGAVVVLLKAR"
     gene            complement(721..1059)
                     /locus_tag="VFY80_10155"
     CDS             complement(721..1059)
                     /locus_tag="VFY80_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6008318.1"
                     /translation="MKKIEAIFKPFKLDEVREALSEIGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVPDKLLDGAIDAIVKAARTGKIGDGKIFVTGVDQVIRI
                     RTGETDEAAI"
     gene            complement(1167..2849)
                     /locus_tag="VFY80_10160"
     CDS             complement(1167..2849)
                     /locus_tag="VFY80_10160"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX6008319.1"
                     /translation="MKIAIAQMNVTVGDMPGNVERIVDFAARARAAGASLMVTPELAL
                     CGYPPEDLLLRPDFIESCARALEDLAKRARGIALVVGHPRVAAERRYNSASIVRDGRI
                     AGVYDKHNLPNYTVFDEARYFESGSAPCVFAVNGVQFGVNICEDTWGAAAGVHRVVAD
                     EVAIGVNICADSWRAEAPRAAREAGAGVLLVLNASPYHLGKQHVRYDVMRERVAETGV
                     PLIYANLVGGQDELVFDGASFVLDRQGNLTHQLPWFEEALGIVTLTDGEPQAGERAPV
                     LSTEAEVYRALTMGVHDYVVKNGFPGVLLGLSGGIDSALTLAVAADALGPERVRAVMM
                     PSQYTASMSREDARAQAEALNVRYTEIAIRPIFDAFRAALAAEFKGLPEDTTEENLQS
                     RIRGTLLMALSNKSGAIVLTTGNKSEMGTGYATLYGDMAGGFGVLKDINKMLVYRLAH
                     YRNSIAPVIPKRVIERAPSAELKPDQTDQDSLPPYETLDAILEAYVENDQNPQEIVAQ
                     GFAAADVKRVVNLVRVSEYKRRQAPIGIRITNRGFGKDWRYPITNKYRHRFD"
     gene            2882..4018
                     /locus_tag="VFY80_10165"
     CDS             2882..4018
                     /locus_tag="VFY80_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6008320.1"
                     /translation="MTIEVATSLAGVAPEEWNRLAGDNPFLRHEFLSALHETGCADPA
                     SGWAPHHLLLRREGALAAAMPLYLKDHSYGEYVFDWAWADAYHRHGLDYYPKLVCAVP
                     FTPVTGMRALAAAPRDRARLITAALALARESGVSSLHCLFPTSEEAQHMAEHGMMLRH
                     GVQFHWMNRNYQSFDEFLADMNHDKRKKIRQERRKVREAGIEFRWVEGQEIGRDEWMF
                     FNRCYRRTYREHHSTPYLNLDFFLRIGQTMPQHLVLIMAYRNGRPLAASLNVRNATRL
                     FGRYWGTLEYHAGLHFETCYYQGIEYCIARGLAVLEGGSRGEHKLARGLMPAATVSAH
                     WLAHPQFSAAVEAFLAREAGSMAHYVDELNERQPFKHQLQADAP"
     gene            complement(4039..5001)
                     /locus_tag="VFY80_10170"
     CDS             complement(4039..5001)
                     /locus_tag="VFY80_10170"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX6008321.1"
                     /translation="MWRVPAIFSSALALAVLAPLALAALAPGDYEVPITHKALSRSYL
                     LHVPPHATSGTALPVVINFHGGGSNARTQKWYSRMDAASDRDGYIAVYPNGSGGFGTR
                     FLTWNAGNCCGIAALNGADDVGFTLAVVDDLAARTPIDRARVYATGLSNGSMMAYRLA
                     ADASERIAAVAGVAGAMTLPDFRPKLPVPVMHIHSLDDERALYAGGLGPAFPQTDTRV
                     FHPPVEDMLKKWIEHNGCPREGRVADLVQGAPGFDDRHTANRIVHAPCRDGVEVVLWK
                     LAGPGHVWPGGRRNFLPVLLGPSSAVIDANREMWAFFSRFRRAR"
     gene            complement(5003..>5542)
                     /locus_tag="VFY80_10175"
     CDS             complement(5003..>5542)
                     /locus_tag="VFY80_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6008322.1"
                     /translation="MWELYAFWAFVPFVLTAHLGSNASGPLNVSLWSFFVIAAGFAGC
                     AVGGLLSLKVGSARVAFAQLLASGLCCVISPFLFHAPTPLFLAFLLLWGIVVVGDSPQ
                     FSALNAQNASPGLVGSALTIANSIGFAISILSIQLLNVLSARIPVEYLFVPLAIGPIL
                     GLLALRPLLRPMTALASID"
BASE COUNT         1019 a         1838 c         1743 g          942 t
ORIGIN      
        1 gacggcaacg agcgtgccgg gcgtcttcgc cgcaggagat gtgcaggatc acgtctatcg
       61 gcaggcggtg acgagcgccg gcagtggctg catggcggcg ctcgacgcgc agaagtacct
      121 ggaaggcaag ggcctcgttt agccgcccgg ctcgttcact gcatggccag ctatgaaaaa
      181 ggatcgatcc aagccgatgg cggaagaact cgaggcactg cgtgcgctcc tgccggacgt
      241 gacgccgatc ccgcgcgcga accgcgcgag cgttgcgccg cccccgcctg cgccggtcgc
      301 cttgcagcgg ctgcgcgatg agcgcgccgc cctagccgat agcctcaacc cccgcgatcc
      361 gtgggaaagc ggcctcgaga cgggagatga gctcagcttt ctgcgcccgg gccttgcgcc
      421 gatcacgctg cgcaagctgc gccgcgggca ctgggtgatc caggacgaaa tcgatctgca
      481 cgggctcacc acgccgcagg cgcacgaact gctcgcaggc tttctcggcg caagcctgcg
      541 ccgcggcctg cgatgcgtgc gtgtgatcca cggcaagggg ctgcgctcga aaaaccgcga
      601 gccggtcctc aagaacaagg tcgcgcgctg gctgatgcag cggcaggagg tgctggctta
      661 ctgtcaggca cgccggaccg agggcggcgg cggcgcggtg gtggtgctgc tcaaggcgcg
      721 ctagatcgcc gcctcgtccg tctctccggt acggatacgt atcacctggt cgacaccggt
      781 cacgaagatt ttcccgtccc cgattttgcc ggtgcgcgcg gccttgacga tggcgtcgat
      841 cgccccgtcg agcagcttgt ccgggacgac gacttcaact ttcactttcg gcaggaagtc
      901 gaccacgtat tcggcgcccc gatagagttc ggtatggccc ttctgccgcc cgaaaccctt
      961 cacctcggtg acggtgagcc cgctcacccc gatctcggac agcgcctccc gcacctcatc
     1021 gagtttgaac ggtttgaata tcgcctcgat cttcttcatc tgctcctccg caatgcgggc
     1081 aaagctcggc aggcgcaatg ccgctcgcgc gacccgtatt tccgctggca agcctgtaag
     1141 gatatcacag ccggcggagc agccgctcag tcgaaacgat ggcggtactt gttggtaatg
     1201 ggatagcgcc agtccttgcc gaagccgcgg ttcgtgatgc ggatcccgat cggcgcctgg
     1261 cggcgcttgt actcgctcac acgcaccaga ttcacgacgc gcttcacatc cgccgccgca
     1321 aaaccctgcg cgacgatttc ctggggattc tggtcgttct cgacatacgc ttcgaggatg
     1381 gcgtcgagcg tttcatacgg tggcaggctg tcctggtccg tctgatccgg tttcagttcg
     1441 gcggaaggcg cgcgttcgat gacgcgcttc gggatgaccg gcgcgatgct gttccgataa
     1501 tgcgcaagcc ggtacaccag catcttgttg atgtccttca gcacgccgaa gccacccgcc
     1561 atgtcgccgt aaagcgtcgc gtatccggtg cccatttcgc tcttgttgcc ggtcgtgagc
     1621 acgatggcgc cgctcttgtt ggagagcgcc atcaacagcg tgccgcggat ccgggactgc
     1681 agattctctt cggtcgtgtc ttcggggagc cccttgaact ctgcggcaag cgcggcgcga
     1741 aaggcatcga agatcggcct gatcgcgatt tcggtatagc gcacgttgag cgcctcggcc
     1801 tgcgcgcgcg cgtcctcgcg gctcatgctc gctgtgtatt gcgagggcat catcaccgcg
     1861 cgcacgcgct caggtcccag cgcgtcggcc gccaccgcaa gcgtgagcgc ggaatcgatg
     1921 ccgcccgaga ggcccagcag cacgccggga aagccgttct tcacaacgta gtcgtgcaca
     1981 cccatggtaa gcgcccggta gacttccgcc tcggtggaca ggactggcgc ccgttcgccg
     2041 gcctgcggtt cgccatcggt cagcgtgacg atgccgagcg cttcctcgaa ccagggcagt
     2101 tgatgcgtga ggttgccctg gcgatcgagc acgaacgatg cgccatcgaa caccagttcg
     2161 tcctgcccac ccacgagatt ggcatagatg agcggcacgc cggtctccgc gacgcgttcc
     2221 cgcatcacgt cgtagcgcac gtgctgcttg cccaggtgat agggcgacgc attcagcacc
     2281 agcaatacgc ctgcgcccgc ctcgcgcgct gcgcgcggcg cctccgcacg ccatgaatcg
     2341 gcgcagatgt tgactccgat cgcgacctcg tccgccacta cgcggtgcac gccggcggcc
     2401 gctccccagg tgtcctcgca gatattgacg ccgaactgca cgccatttac cgcgaacacg
     2461 catggcgccg aaccggattc gaaatagcgc gcttcgtcga acaccgtata gttcggcaga
     2521 ttgtgcttgt cgtagacgcc cgcgatgcgg ccatcgcgaa cgatcgaggc ggagttgtag
     2581 cggcgctccg ccgcgacgcg cggatgaccg acgaccagcg cgatgccgcg cgcccgcttc
     2641 gcgaggtcct ccagcgcgcg cgcacacgat tcgatgaagt ctgggcgaag cagtaggtct
     2701 tcgggcggat aaccgcagag cgcgagttca ggggtcacca tgaggcttgc acccgccgca
     2761 cgcgcgcgcg cggcgaagtc gacgatgcgc tccacgtttc ccggcatgtc gccgactgtg
     2821 acattcatct gcgcgatcgc tatcttcatg cgccgaatga tatcatcgcg acgcactcac
     2881 cgtgaccatc gaagtcgcaa cctccctcgc gggcgtcgcg cccgaagaat ggaatcgcct
     2941 ggcaggcgac aatcctttcc tgcgccacga atttctatcg gccttgcacg agaccgggtg
     3001 cgccgaccct gcgagcggct gggcgccgca ccatcttctt ctgcggcgcg agggtgcact
     3061 cgccgccgcc atgccgctct atctcaagga tcattcgtac ggggagtatg ttttcgactg
     3121 ggcgtgggcc gatgcctatc atcggcacgg gctcgactac taccccaagc tcgtgtgcgc
     3181 cgtcccgttc acaccggtga caggtatgcg cgcgctcgca gcggcaccgc gcgatcgcgc
     3241 acggctcatc accgctgcgc tcgcgctcgc gcgcgagtca ggcgtgtcct cgctgcactg
     3301 cctgttcccc acatccgaag aggcgcagca catggcggag cacggcatga tgctgcgcca
     3361 tggcgttcag tttcattgga tgaaccgcaa ctaccagagt ttcgacgaat tccttgccga
     3421 catgaaccac gacaagcgca agaagatccg ccaggagcgt cgcaaggtgc gcgaagcggg
     3481 catcgagttt cgctgggtgg agggacaaga gatcggcagg gacgagtgga tgtttttcaa
     3541 ccgctgctat cgtcgaacct accgcgaaca ccattccacg ccctacctca atctcgactt
     3601 ctttctccgc atcgggcaga cgatgccgca gcatctcgtg ctgatcatgg cctatcgcaa
     3661 cggccgcccg ctcgccgcct cgctcaacgt gcggaatgcg acgcggctat tcggacgcta
     3721 ctggggaacg ctcgagtatc atgcggggct tcatttcgag acctgctact accagggcat
     3781 cgaatactgc atcgcccgcg gactcgcagt gctcgaaggc ggctcgcggg gagagcacaa
     3841 gctcgcccgc ggtctcatgc ccgccgccac ggtctccgct cattggctcg cgcacccgca
     3901 gttcagcgcc gcggtcgaag cctttcttgc acgtgaggca ggaagcatgg ctcattacgt
     3961 cgatgagctg aatgaacgcc agccgttcaa gcatcagctc caggcggacg ccccctaacc
     4021 tagaacactc cgaccgatct accgggcacg gcgaaaacgc gagaaaaacg cccacatctc
     4081 gcggtttgcg tcgatcaccg cactcgaggg gccgagcagc accggcagga aattccggcg
     4141 gccgcccggc cagacgtgcc ccggacctgc gagcttccaa agcacgactt cgacgccatc
     4201 gcggcaaggc gcgtgaacga tccggtttgc ggtgtgccgg tcgtcgaacc cgggcgcgcc
     4261 ctggacgaga tcggccaccc taccctcacg cgggcatccg ttgtgctcga tccacttttt
     4321 cagcatgtct tcgacgggtg gatgaaaaac gcgcgtatcc gtctgcggaa acgcaggacc
     4381 gagtcccccc gcatataagg cgcgctcgtc gtcgaggctg tggatatgca tcaccggcac
     4441 gggaagcttc ggtcggaaat cgggcaacgt catcgcgccc gccaccccgg ctacggccgc
     4501 gattcgctct gacgcgtccg ccgcgaggcg atacgccatc atcgacccgt tggaaagtcc
     4561 ggtcgcgtac acgcgtgccc ggtcaatggg cgtcctcgct gcaagatcgt cgactacagc
     4621 gagcgtgaac cccacgtcat cggctccgtt cagtgcggcg atcccgcagc agttgcctgc
     4681 gttccaggtg agaaaccgtg tgccaaagcc gccgctgccg ttcgggtaga ccgcgatgta
     4741 gccgtcgcgg tcggacgcgg catccattcg gctgtaccac ttttgcgtgc gggcgttgct
     4801 gccgccaccg tgaaagttga tcaccaccgg aagtgcggta cccgaggtcg cgtgcggcgg
     4861 cacgtgcagt agatagcttc gcgagagcgc tttgtgtgtg atcggaacct cgtaatcgcc
     4921 gggcgcgagc gcggccagcg cgagtggtgc gagcacggcg agcgcgaggg cgctggaaaa
     4981 aatcgccgga acgcgccaca tctcagtcta tcgacgccaa cgcggtcatc ggcctcagca
     5041 gcggccgcaa cgcgagaaga cccaagatag gcccgatcgc cagtggcacg aagagatact
     5101 ccacggggat gcgcgctgaa agcacattca ggagctgtat gctcagaatc gagatcgcaa
     5161 agccgatgct gttggcgatg gtgagcgcgg atcccaccag cccgggagag gcgttctgcg
     5221 cgttgagcgc agagaattgc ggagaatcgc cgacgaccac gatcccccac aacagcagaa
     5281 aggcaaggaa gagcggcgtg ggcgcatgga acagaaacgg cgaaatcaca cagcagagcc
     5341 ccgaggccag caactgcgca aacgcgacgc gagcgctgcc gaccttgagt gacaggaggc
     5401 cgcccacggc gcagcccgca aagcccgcgg cgatgacgaa gaacgaccag agcgaaacgt
     5461 tgagcgggcc ggaggcgttc gaaccgagat gcgcggtcag cacgaacgga acgaatgccc
     5521 agaacgcgta gagctcccac at
//
LOCUS       DASURG010000563         5153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1320994, whole genome shotgun sequence.
ACCESSION   DASURG010000563 DASURG010000000
VERSION     DASURG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320994"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VFY80_10180"
     CDS             complement(<1..1225)
                     /locus_tag="VFY80_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX6008323.1"
                     /translation="MLAKLEQSAAALTAADASRARHLLFVLPPAKERRALAGVPFADA
                     LEAALRRRRKKLDELAKSPVAVDLPHGALASWVALEGAQTPFEQYSHMRKALQPLLAE
                     KPTEIVVAVFGEGPARRLAAERAVYAAWVNGAPLPERKKKPEAVPLERIRLHGHRDPA
                     RFALLHAQAMGNTLCRELTVLPPNELNPGAYRQRIARLAREHGWARDEYDVKRLRAMG
                     AGAFVAVAQGSATQDAAIVRLRYRARQAKKTLALVGKGICFDTGGHNLKTARYMHGMH
                     EDMNGSAVVLGILLAATEAQLPVNLDCWLAIAQNHLSPQAYKQNEVITALNGTTIEIV
                     HTDAEGRLVLADALTLAARAKPDAMVDFATLTGSMHVALGDRYSGFFATSDELAARAL
                     AAGVASGERVWRFPLD"
     gene            1342..2136
                     /locus_tag="VFY80_10185"
     CDS             1342..2136
                     /locus_tag="VFY80_10185"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX6008324.1"
                     /translation="MRQYLDLMQHVLEKGVRKSDRTGTGTLSVFGAQLRFDLAAGFPL
                     LTTKKVHLRSIIHELLWFLKGDTNVKYLRDNGVTIWDEWANESGDLGPVYGYQWRSWP
                     AADGRHIDQMSQVLDALRRNPDSRRLIVSAWNVGDLERMALMPCHAFFQFYVAGGRLS
                     CQMYQRSADLFLGVPFNIASYALLTLMVAQVCGFKPGEFIHTFGDTHLYLNHLDQARE
                     QLARTPRRLPVMRLNPAVNDLFGFRYEDFTLEGYDPHPVIKAPIAV"
     gene            2162..2665
                     /locus_tag="VFY80_10190"
     CDS             2162..2665
                     /locus_tag="VFY80_10190"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX6008325.1"
                     /translation="MTGKSTARISLVVAMARNRVIGAGGRIPWRLPDELRLFRDITMG
                     HHMIMGRKTYESIGRLLPGRTTVIVTRQPGYTVPGAIIAHSLDEAVVACGSDDEIFVI
                     GGAELYRAALPRADRIYLTTVDAEPVGDTFMPEVDLAAWLEISSDAFPADAKHAHPWR
                     FAIYDRK"
     gene            complement(2711..3898)
                     /locus_tag="VFY80_10195"
     CDS             complement(2711..3898)
                     /locus_tag="VFY80_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX6008326.1"
                     /translation="MVVRSVALKKQQPAFENDPQLFDTHPEVRLDFDRVREALQQGYT
                     RPFLLVDTEIVRTKARRFKAAMPRVHPHYAVKANPDPRVLKALIEEGVGFEIASIAEL
                     DLLLGLGVPAAEVYYSNPMKSRAYIEYAAEKGVEWYVLDSVEELRKIVSVKPDAKLYV
                     RIEAPNVGSDWPLAGKFGIKSTEVDGIIAEAVTLKADLAGVTFHVGSQCRNPENWRVG
                     IQHAKRVFRRMRLAGLTPRLLNIGGGYPVRHVKPIPSIERIADVVNHGIRDIPQTVRI
                     MAEPGRFLVSDAGYFVCRVVGTATRGGKRWMYWDAGVFGGVIETTEGLRYDIHTDRTG
                     KLIPWCIAGPTCDSVDVCMRDELFPEDMQEGDFVYIANAGAYTTAYASNFNGFPLPEV
                     RVI"
     gene            complement(4213..4785)
                     /gene="dcd"
                     /locus_tag="VFY80_10200"
     CDS             complement(4213..4785)
                     /gene="dcd"
                     /locus_tag="VFY80_10200"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX6008327.1"
                     /translation="MTIKSDRWIRRMAERHRMIDPFEPSQVKYADGRRLVSYGTSSYG
                     YDIRCSNEFKIFTNINTTIVDPKNFDERSFVDFRGDVCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPLEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVIFL
                     ESAADDICQTSYKDRGGKYQGQTGVTLPKI"
     gene            complement(5021..>5153)
                     /locus_tag="VFY80_10205"
     CDS             complement(5021..>5153)
                     /locus_tag="VFY80_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX6008328.1"
                     /translation="VVSDPDGRIAQIYKQIARRVAIQIAERQHDHSAVFPKIVVQNT"
BASE COUNT          907 a         1656 c         1633 g          957 t
ORIGIN      
        1 cgtcgagcgg aaagcgccat acccgctcac cggatgccac gcccgccgcg agcgcgcgcg
       61 cggcaagctc gtcgctcgtg gcgaagaagc cgctgtagcg atcgcccaag gcaacatgca
      121 tgctgccggt gagcgtcgcg aaatcgacca tcgcgtccgg cttcgcgcgc gccgcgagcg
      181 tgagcgcatc ggcaagcacg agccgccctt ccgcatcggt gtgcacgatt tcgatcgtgg
      241 tgccgttgag cgcggtgata acttcgtttt gcttgtaggc ctgcgggctc aaatggttct
      301 gcgcgatcgc gagccagcaa tcaagattga ccggcaattg tgcttcggtt gcggcgagca
      361 ggatgccgag cacgacggcc gagccgttca tgtcctcgtg catgccgtgc atgtagcgcg
      421 cggtcttcag attgtggccg ccggtgtcga agcagatccc tttgccgacg agggcgaggg
      481 tcttcttcgc ctgccgggcg cggtagcgca gccgcacgat ggcggcatcc tgcgttgcgc
      541 tgccctgcgc gacggcgacg aatgcgcccg cgcccatcgc gcgcagccgc ttgacgtcgt
      601 actcgtcccg tgcccagccg tgctcgcgtg cgagccgcgc gatgcgctgc cggtacgcgc
      661 cgggattgag ctcgttcggc ggcagcacgg taagctcgcg gcagagcgtg ttgcccatcg
      721 cctgggcgtg gagcaacgcg aaacgggcgg gatcgcggtg cccgtgcagt cgtatgcgct
      781 ccagcgggac cgcctcgggc ttcttcttcc gctcgggcaa cggcgcgccg ttcacccagg
      841 ccgcatatac tgcgcgttcg gcggcgagcc ttctcgcggg accctcgcca aataccgcga
      901 cgacgatctc cgtcggtttc tcggccagaa gcggctgcag cgccttgcgc atgtgcgagt
      961 actgctcgaa tggtgtctga gcgccctcca acgcgaccca agacgcgagc gcgccatgag
     1021 gaagatccac cgccaccggc gattttgcga gctcatcgag ctttttccgc cggcgccgaa
     1081 gcgcagcctc gagcgcgtcg gcaaagggca cgcccgcgag cgcccgccgc tcctttgcgg
     1141 gcggcagaac gaagagcagg tggcgcgcgc ggctcgcgtc ggcggcggtc aaggcggcgg
     1201 cggactgttc gagtttcgcg agcatggaat acagtcggtc gaatcccggg cgaccggcgg
     1261 attataaccg cgccccgcgc cacgcctgcc ggtccaattc gattagaatc agcacgactc
     1321 gcaaaccccg cgccatccat catgcgtcaa tatctcgacc tcatgcagca cgtgctcgag
     1381 aagggcgtgc gcaagtccga ccgcaccggc accggcacac tcagtgtttt cggggcacaa
     1441 ctgagattcg atctcgccgc cgggtttccg ctgctcacca ccaagaaggt gcatctcagg
     1501 tcgatcattc acgaactgct gtggttcctg aagggcgata ccaacgtcaa atacctccgg
     1561 gacaacggcg tgaccatctg ggacgagtgg gccaatgaga gcggcgatct aggaccggtc
     1621 tacggctacc agtggcgctc atggccggcg gcggacgggc ggcacatcga ccagatgagc
     1681 caggtcctcg acgcgctcag aaggaatccg gattcgcgcc ggctcatcgt ttccgcgtgg
     1741 aacgtcggag acctcgagcg catggcgctc atgccttgcc acgcgttctt ccagttctat
     1801 gtcgccggcg gcaggctttc gtgccagatg taccagagaa gcgccgacct tttcctcggc
     1861 gtgccgttca acatcgcctc ttatgcgctg cttacgctga tggtcgcgca ggtgtgcggc
     1921 ttcaagccgg gcgaattcat ccataccttc ggcgacacgc acctctactt gaatcacctc
     1981 gaccaggcgc gggagcagct cgcccgcacg ccgcgccgcc tgccggtcat gcggctcaat
     2041 cccgccgtga acgacctctt cggcttccgc tacgaagact tcacgctcga gggctacgat
     2101 ccgcacccgg tgatcaaggc gccgattgcg gtctgagcgg cccgcatccg gaacggctcg
     2161 ggtgacggga aaatcgaccg cgcgtatctc ccttgtcgta gctatggcgc gcaatcgcgt
     2221 gatcggcgcg ggcgggcgca ttccctggcg cctgcctgat gaactccggc tcttcagaga
     2281 catcacgatg gggcatcaca tgatcatggg gcggaagacc tacgaatcga tcggccggct
     2341 gcttcccgga cgcacgacgg tgatcgtgac ccgccagccg ggctacaccg ttcccggcgc
     2401 gatcatcgcc cactcgctcg acgaagccgt tgtggcatgc ggctcggacg atgagatctt
     2461 cgtgatcggt ggcgcagagt tgtatcgcgc ggcgctcccc cgggcggatc gcatctacct
     2521 cacgaccgtc gatgccgagc cggtaggcga cacgttcatg cccgaagtcg atttggcggc
     2581 ctggctcgag atttcgagcg acgcctttcc tgccgatgcg aagcacgccc acccctggcg
     2641 ctttgcgatc tacgaccgca aataaaaagg gccaggttac aagcctggcc cttgctggag
     2701 cgcacgcgcc ctagatcacg cgcacttccg gcagcggaaa gccgttgaaa ttgctggcgt
     2761 acgcagtcgt gtaggcgccc gcattggcga tatagacgaa gtcgccctcc tgcatgtcct
     2821 cggggaacag ctcgtcgcgc atgcacacgt ccaccgagtc gcacgtcggc ccggcgatgc
     2881 accacggaat gagcttgccg gtccgatccg tgtggatgtc ataccgcaac ccttcggtgg
     2941 tctcgatcac gccgccgaac acgcccgcat cccagtacat ccagcgcttc ccgccacggg
     3001 tcgcagtgcc gacgacgcgg cagacgaaat agccggcgtc ggagaccagg aagcgaccgg
     3061 gctcggccat gatccttacc gtctgcggga tgtcgcggat gccgtggttg acgacgtcgg
     3121 cgatccgctc gatcgagggg atcggcttca cgtggcgcac gggatagccg ccgcctatat
     3181 taagaaggcg cggcgtcagt ccggcgagcc gcatcctgcg gaacacgcgc ttggcgtgct
     3241 ggatcccgac gcgccagttc tccgggttgc ggcattgcga gcccacgtga aaggtcacgc
     3301 cggcgaggtc tgccttgagc gtcaccgcct ccgcaatgat gccgtccact tccgtgctct
     3361 tgatgccgaa cttgccggca agcggccaat cgctgccgac gttcggcgcc tcgattctca
     3421 cgtagagctt ggcgtccggc ttcacgctca cgatcttgcg caactcttcc acgctgtcga
     3481 gcacgtacca ctccacgccc ttctccgccg cgtactcgat gtacgcccgc gatttcatgg
     3541 ggttcgagta gtagacttcc gccgccggga cacccaggcc gagcaggagg tcgagctcgg
     3601 cgatcgacgc gatctcgaag ccgacgcctt cctcgatcaa cgccttcagc acgcgcgggt
     3661 cggggttcgc cttgaccgcg tagtgcggat gcacgcgcgg catggcggcc ttgaagcggc
     3721 gggccttggt tcgaacgatt tcggtatcga ccagaaggaa gggcctggtg tagccctgct
     3781 ggagcgcttc ccggacgcgg tcaaagtcca ggcgtacttc ggggtgggtg tcgaagagct
     3841 gcgggtcgtt ctcgaaagcg ggttgctgtt tcttgagggc gacagagcgc acgaccatca
     3901 aagttctccc tgggacctaa gattcgggta tcaaaaaagt cacagcctca accgtcctgc
     3961 actctttcat tacgcctctc atgatgacct ccttagcgcc agtgtcgctt gcggggcaga
     4021 gccaaatttt cgcgaattat aggagctatt tcccaggaat caagcgattt ggacggcctt
     4081 tcggaaaaaa aattcagtgc gccgaaacgc cggtatccgg tgatggcgaa agctcgccgg
     4141 gaaagcgctc cgcgccgcta ctcgcgtgtt gcgagcgctt ctcagttgaa gggaagcgaa
     4201 ccgccgtgca ggtcagatct tgggcagtgt gacgcccgtt tggccctgat atttcccgcc
     4261 gcggtctttg taggaggtct ggcagatgtc gtccgcagcc gattcgagga agatcacctg
     4321 cgcgacgccc tcgttggcgt aaatcttggc gggaagcggc gtggtgttgg agaactccag
     4381 cgtaacgtag ccttcccact ccggctcgag cggcgtcacg ttcacgatga tgccgcagcg
     4441 ggcgtaggtg ctcttgccca agcagatcgt gagcacgttc cgcggaatcc ggaaatattc
     4501 gacagtacgg gccagcgcga aggaattggg cgggatgatg cacacatctc cgcggaagtc
     4561 gacgaaggag cgttcgtcga agttcttggg atcgacgatg gtcgtgttga tgttggtgaa
     4621 gatcttgaat tcgttcgagc agcggatgtc gtagccgtag cttgacgtgc cgtaggagac
     4681 gaggcgtcgc ccgtccgcat acttgacctg cgagggctca aacggatcga tcatgcgatg
     4741 gcgctccgcc atgcggcgga tccaccggtc ggatttgatg gtcattgttg taatggcgag
     4801 ggcagaacgc gttgggcgat tataaggcca tcccgccaca cccagcgcta cgaccgcttc
     4861 tgccaaccac tcatcccccc ggcgaaacgc gcttcagcca cccagtcgtc tccaggcgaa
     4921 gcgggcttgt gccaaccgtt cgtctccccg gcgaaagccg gggtccagaa acggactgga
     4981 tttcgccttt tccctgagcg acgaaccacg aggccgggtg ctaggtgttc tgcaccacaa
     5041 ttttggggaa taccgccgaa tgatcgtgct gccgctcggc gatctggatc gcgacgcggc
     5101 gcgcgatctg cttgtagatc tgcgcgatgc ggccgtccgg atccgagacc aca
//
LOCUS       DASURG010000564         4902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_911988, whole genome shotgun sequence.
ACCESSION   DASURG010000564 DASURG010000000
VERSION     DASURG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911988"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /gene="gcvT"
                     /locus_tag="VFY80_10210"
     CDS             <1..886
                     /gene="gcvT"
                     /locus_tag="VFY80_10210"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX6008329.1"
                     /translation="SMLANDVAKLAARGKALYSCMLDAHGGVLDDLIVYFLDSTHYRI
                     VVNAGTAGKDLAWLREHAARATQPVELAPRHDLALIAVQGPNAREKTWRALPQIRADT
                     EALAVFQGAETDNMLIARTGYTGEDGFELMLDAARAEEVWHSLQAQAVPPAGLGARDT
                     LRLEAGMNLYGQDMDETVTPFEAALAWTVDLNQGRDFIGRSALVSRPARWQLLGLVLL
                     ERGVMRAHQRVRTVTGDGVVTSGSFSPTLGRSIALARLPTDLSAGDGVEISVRDRWIP
                     ARVVKPPFVRYGTIRVTG"
     gene            906..1295
                     /gene="gcvH"
                     /locus_tag="VFY80_10215"
     CDS             906..1295
                     /gene="gcvH"
                     /locus_tag="VFY80_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX6008330.1"
                     /translation="MTTPNDLKYTASHEWARQEPDGTVTIGITFHAQEMLGDVVFIEN
                     PAVGRKLKQGEECGVIESVKAAADIYAPIAGEVIASNTELNDAPEQVNKDPYGAWMFR
                     LKPEDPSQVATLLDAAAYRKIAESEKR"
     gene            1305..2636
                     /gene="gcvPA"
                     /locus_tag="VFY80_10220"
                     /pseudo
     CDS             1305..2636
                     /gene="gcvPA"
                     /locus_tag="VFY80_10220"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
     gene            2718..3218
                     /gene="tpx"
                     /locus_tag="VFY80_10225"
     CDS             2718..3218
                     /gene="tpx"
                     /locus_tag="VFY80_10225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX6008331.1"
                     /translation="MANVTLGGNPITVAGKLPAKGELAPDFTLTSKDLKDIGLKDLAG
                     KRKVLNIVPSLDTPVCAKSTRIFNERASGMANAVVLVISADLPFAMNRFCGAEGIANV
                     VTLSTVRNRDFHAKYGVDIADGPLKGLTARAVVVLDEGNRVLYSQLVPEIKSEPDYDA
                     ALAALK"
     gene            3266..4729
                     /gene="gcvPB"
                     /locus_tag="VFY80_10230"
     CDS             3266..4729
                     /gene="gcvPB"
                     /locus_tag="VFY80_10230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6008332.1"
                     /translation="MLIFEHSRPGRENASQAPRRADLRRAIPDRFLRAGTPLLPEVSE
                     LDAVRHYTALSQKNYAIDTHFYPLGSCTMKYNPRACNALAMLAQFLERHPYAPDDTGQ
                     GFLACMLELQEILKEVTGMAAVSLAPMAGAQGEFAGVAMIRAYHDARGDGDRREILVP
                     DAAHGTNPATAVMCGYAVREIPTDAAGNVDLGALGAAVGPHTAGLMLTNPSTLGVFEK
                     SILDIATIVHDAGGLLYYDGANLNAILGRAKPADMGFDAIHINLHKTFSTPHGGGGPG
                     AGPVGVTSRLAPFLPLPVVARRDGACRLLGENDMPLSIGRLGAHLGNAGVLLRAYVYA
                     RLLGAEGMRRVADFATLNANYLMVELGRAGFEVAYPTRRASHEFIVTLRRLKEKTGVT
                     AMDVAKRLLDKGFHAPTSYFPLLVPECLLIEPTETESKQTLDAFVAAMKEILVEAHEN
                     PQLVKTAPHTTPVRRLDDVRAARELDLAWRPGGAASA"
     gene            complement(4719..>4902)
                     /locus_tag="VFY80_10235"
     CDS             complement(4719..>4902)
                     /locus_tag="VFY80_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEX6008333.1"
                     /translation="FNYGIFINNVISFIIVAFAVFLLIRAINRLRRREEEKPAAPPAP
                     PEEVTLLREIRDALKR"
BASE COUNT          860 a         1564 c         1622 g          856 t
ORIGIN      
        1 atcgatgctc gccaacgatg tggcgaagct tgcagcgcgt ggcaaggcgc tctattcatg
       61 catgctcgat gcgcacgggg gcgtgctgga cgacctcatc gtctattttc tcgatagcac
      121 ccattaccgg attgtcgtca acgccgggac ggctgggaaa gacctcgcct ggttgcgcga
      181 gcacgctgcg cgcgcgacac agccggtgga gctcgccccg cgtcacgacc tcgcgctcat
      241 tgccgtgcag ggacctaacg cgcgggagaa aacgtggcgg gcgctgccgc agattcgagc
      301 ggacacggag gcgctcgctg tatttcaggg agccgaaacc gacaacatgc tgatcgcgcg
      361 caccggctat accggggaag acgggttcga gctgatgctc gacgcggcgc gcgccgagga
      421 agtgtggcat tcactgcagg cgcaagcggt gccgcccgcc gggctcggtg cgcgcgatac
      481 cctgcggctc gaggccggaa tgaaccttta cgggcaggac atggacgaaa ccgtcacacc
      541 gttcgaagcg gcgctcgcat ggacggtgga tctcaaccag gggcgggatt tcatcggcag
      601 atcggcgctc gtctcacggc cggcgcgttg gcagcttctg gggctcgtgc ttctggagcg
      661 cggcgtcatg cgcgcgcacc agagggtgcg aactgtgaca ggggacgggg ttgtgacgag
      721 cggcagcttc tctcccacgc tcgggcgctc catcgcgctt gcgcggctgc cgacggacct
      781 ctcggcggga gatggcgtcg aaatcagcgt gcgcgacagg tggatcccgg cgcgagtcgt
      841 gaagccgcct ttcgtgcgct atggaacaat tcgtgtaacc ggttaagctc gacaaagggg
      901 gaaggatgac cacaccgaat gacctcaagt acacggcgag ccacgagtgg gcgcgccagg
      961 aacccgatgg aacggtgacg atcggcatca cctttcacgc ccaggaaatg ctcggtgacg
     1021 tcgtcttcat cgagaatccc gccgtcgggc gcaagttgaa gcagggtgag gagtgcggcg
     1081 tgatcgagtc ggtgaaggcg gcagccgata tctacgcgcc catcgcgggc gaagtgatcg
     1141 cctcgaatac cgaacttaac gatgcgccgg agcaagtgaa caaggatcca tacggtgcgt
     1201 ggatgttcag gctgaaaccg gaggacccct cgcaggtggc gacgctgctt gatgcggcgg
     1261 cctatcggaa gatcgctgaa tccgaaaagc gctgacgctg caccatgccg ttcgtcccgc
     1321 acaccgacga cgatgtccgc gccatgctgg cggcgatcgg agtcgcatcg atcgatgaac
     1381 tgttcgacga aattccgccg gcgctccgcg ctgcccggcc gctcgacctg caggtcgcag
     1441 cgagcgaaat ggaactcgcg cggttgatgc gcgaacgggc ggagcacgat ggcacgtacc
     1501 tcaatttcat cggtgccggt gcctacgagc accacattcc ggcggcggtg tggcagatcg
     1561 ccacgcgcgg tgagttctat tcatcgtaca cgccctatca ggcggaggcg agtcagggca
     1621 ccttgcaggt gctctacgag taccagacga tgatggcttc cctcctggcg ctcgatgcga
     1681 cgaacgcgag cctctacgat ggcgcctccg cactggcgga agccgtgttg atggcggtgc
     1741 gcgcgcataa gcgcgcgagg cgcgtgctcg ttccccgtac ggtccatccc aggtggcgca
     1801 aggtgacgcg gaccctcacg cgcaatcagg ggatcgagct cgtcgaactg gattacgatc
     1861 gggcgtcggg gcaggtggcc gttgccgcgc tccagcgctt caacggcagg gattttgcgg
     1921 cgctcgtcat tccgcagccc aatttcttcg gcgtgctgga gcctgtggac gaaatggtct
     1981 cgtgggcgca cgcgcacggc atgcttgccg tcgcagtggt gaaccccact gcgcttgcca
     2041 tgctcaaacc gccgggcgag tgggcggcgg gcggtgcaga catcgcagtc ggggaaggcc
     2101 agccgttcgg cgtgccgctc tccggcggtg gaccgtactt cggcttcatg gcgtgccgcc
     2161 aggcgctcgt gcgccagatg cccgggcgtc tcgtgggcgc gacgcgagac gtcgaaggtc
     2221 ggcgcggcta cacgctcacg ctgcaggcgc gcgagcagca cattcgccgt tccaaggcga
     2281 cgtccaacat ctgcacgaac cagggtctca tggtgaccgc tgcgacgctt tacctggcgc
     2341 tcctgggacc atcgggcctt gcgcaggtcg cgcgcacctg tcacagcaac gcgctcgatc
     2401 tcatcgagct cctcactgcg acagcgggcg tggcacgctc gttccaggcg ccgttttttc
     2461 acgagttcgt gatcagcctc gacactccgg ccgcgcgcgt gttgcatgcg ctcgaggcgc
     2521 acggcatact gggcgggctc gacctcgcga gcgattatcc cgagctcggt tcgtctcttc
     2581 tcgtctgtgc gaccgaaacg aagaccgggc aagacctgaa gcgtttttcc gacaccatgg
     2641 cgcgcgttct ctttagggcg tatcggcccg tgccatgtgc gatcaacact ccaccacatc
     2701 tcatggagga tggcccgatg gcaaacgtca cgctcggcgg taacccgatc accgtcgccg
     2761 gaaaacttcc cgcgaagggc gaactcgcgc ccgatttcac tctgaccagc aaggatctga
     2821 aggatatcgg gctcaaggac ttagccggca agcgcaaagt cctcaatatc gtgccgagcc
     2881 tggacacgcc ggtgtgcgcg aagtcgacgc gcatcttcaa cgagcgggcg agcggcatgg
     2941 cgaacgccgt cgtgctcgtc atctcggctg atctcccgtt cgccatgaac cgcttctgcg
     3001 gggccgaggg cattgccaac gtggtgaccc tttccaccgt gcgcaatcgg gattttcacg
     3061 caaagtatgg cgtcgacatc gccgatgggc cgctcaaggg gctcaccgct cgcgcggtgg
     3121 tcgtgctcga tgaggggaac cgtgtccttt attcgcagct cgtcccggag atcaagagtg
     3181 agcccgacta cgatgccgcg ctcgcagcgc tcaagtaagc tgcggcttcg gcattgattc
     3241 gcgctcctgc gacgggcggg ctgccgtgct gatcttcgag cattcgcgtc ccggacgtga
     3301 gaatgcgagc caggcgccgc gacgcgccga cttgaggcgc gccatcccgg accgtttcct
     3361 gcgtgcgggc acgccgcttc tcccggaagt ctcggagctg gatgcagtcc ggcattacac
     3421 ggcgctttcg cagaagaact acgccatcga cacgcatttc tatccgctcg gatcgtgcac
     3481 catgaagtac aacccgcggg cgtgcaacgc gctcgccatg ctggcgcaat tcctcgagcg
     3541 ccatccgtat gcgccagacg ataccgggca gggttttctt gcatgcatgt tggagctgca
     3601 ggagatactg aaggaagtga ccggtatggc ggccgtaagc cttgctccca tggccggcgc
     3661 gcagggtgaa ttcgctggcg tggctatgat ccgcgcctat cacgacgcgc ggggcgacgg
     3721 cgaccggcgc gaaattctcg ttccggacgc cgcgcacgga accaacccgg cgaccgcggt
     3781 gatgtgcggc tatgcggtgc gcgagatccc gacggatgcg gcaggcaatg tcgatctcgg
     3841 cgcgctcggc gctgcggtcg gccctcatac cgcggggctc atgctcacca acccgtccac
     3901 actcggtgtg ttcgaaaaga gcatcctcga catcgccacg atcgtgcacg acgcgggagg
     3961 gcttctgtac tacgacggcg cgaatctcaa cgccatcctc gggcgggcga agccggccga
     4021 catggggttt gacgcgatcc acatcaatct gcacaagacg ttttccacac cgcatggcgg
     4081 gggcggtccc ggcgccggcc ccgtgggcgt gacatcgcgc ctcgcgccgt tcctgcccct
     4141 cccggtggtg gcacggcgcg acggcgcctg ccgactgctc ggcgagaacg acatgccgct
     4201 ttccatcggc cgcctcggcg cgcatctcgg caatgccggc gtcctcctgc gcgcctacgt
     4261 ttacgcccgt ctcctcggcg cggaaggcat gcggcgcgtg gcggattttg cgaccctcaa
     4321 cgcgaattac ctcatggtcg agcttgggcg cgcgggcttt gaagtggcat acccgacacg
     4381 gcgggcgagc cacgagttca tcgtgacgct gcgccggctg aaggagaaga ccggggtcac
     4441 ggcgatggac gtcgcgaagc gcctgctcga caagggcttt catgccccga cgagctactt
     4501 tccgttgctc gtgcccgagt gcctgctgat tgaacccact gaaacggagt cgaagcagac
     4561 gctcgacgcc ttcgtggcgg cgatgaaaga gatcctcgtc gaagcgcacg agaatccgca
     4621 gctcgtcaag acggctccgc acaccacgcc cgtgcgccgg ctcgacgatg tgagagccgc
     4681 gcgcgagctc gacctcgcgt ggcgacccgg gggtgcagct agcgcttgag cgcgtcccgt
     4741 atttcgcgca gcaaggtgac ctcttccgga ggcgcgggcg gcgcggccgg cttttcctct
     4801 tccctgcgcc gcaaccggtt gatcgcgcga atcaggagga acaccgcaaa cgcaacgatg
     4861 atgaagctga tgacgttgtt gatgaagatg ccgtagttga ac
//
LOCUS       DASURG010000565         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1014483, whole genome shotgun sequence.
ACCESSION   DASURG010000565 DASURG010000000
VERSION     DASURG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014483"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1563
                     /locus_tag="VFY80_10240"
     CDS             256..1563
                     /locus_tag="VFY80_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX6008334.1"
                     /translation="MAFKKDTAKVLSIDGREVRVTNPDKPYFSKQIKLSKLDLVRYYL
                     SVAPGALAGIRDRPLVLKRFVDGADGEAFYQKRAPEKRPDWLRTVTLSFPSGRTAEEI
                     VVDDAAGLSWVVNLGCIELHPHPVRSGDLEHPDELRVDLDPGPGVAWDDVRRVALEVK
                     AFLEEMGLRGWPKTSGSRGMHVNVRIEPRWTFVEVRRAALALSRAVERRLPALASSKW
                     WKEERHGVFLDYNQNAKDRTTCSAYSVRPLPDARVSTPLHWHEVPDCDPADFTVLTVP
                     KRLAEIGDPHAEMDSALGSLEKLLDLASQDEAAGLGDAPWPPHFRKMEGEAPRVQPSR
                     ARSNRNKSGTKKPRTRMPLIVVANSPNRQAALAGLERWKAKHPEAAAHLAVDDVLTDS
                     MRGRSSTWTRIRVNLRHVPEELRPPQETPDPDDDPTREWRERS"
     gene            complement(1724..2821)
                     /locus_tag="VFY80_10245"
     CDS             complement(1724..2821)
                     /locus_tag="VFY80_10245"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6008335.1"
                     /translation="MNGPEIMNLPVNPPILPMLAKRAGELPKGENWIFEPKWDGFRAV
                     VFRARDEVLLQSRDEKSLNRYFPELLDPLRAQLPARCVLDGEIVIAQNGALDFDALQL
                     RIHPAASRVTLLSRQTPASIVFFDLLSEGSRDLRSVPFHERRGMLETLLASSTPPLHL
                     TPATSDSSIASDWFRRFEGAGLDGVVAKQAMGIYEPNKRVMVKVKHERDCDCVVAGFR
                     WHKRGERSAVGSLLLGLYDDSGTLQHVGVCASFTDKKRRELVDFLAPYRKNALARHPW
                     KHWAEQGDDGEHRMPGAQSRWSGGKDLSWEPLRPELVVEVAYDHMQGDRFRHTAQFRR
                     WRTDKKPRDCTYDQLEVVPPEELSTIFAGGR"
BASE COUNT          557 a          928 c          903 g          527 t
ORIGIN      
        1 gcaagcgtcg cgttgcggga gacgagcggg gccctgagcc aggggatggg gcggtgattc
       61 accattcaac cggggaagcc gacttcgacg gtctaccggg acatgttccg cctccgatca
      121 aagaccctaa tcgcgatctg ccgtatcctc ataagaaagc cgccgattag ctgttcgtga
      181 tcgtgttcgt gctacgtgtt cgtgctttcg gaatagcacg gcgacgcttc acgatcacga
      241 tcacggttcc gacgcatggc gttcaagaag gacacggcca aggtcctttc catcgacggg
      301 cgcgaagtcc gggtgaccaa ccccgacaag ccgtacttct ccaagcagat aaaactctcg
      361 aagctcgatc tcgttcgcta ttatctttcg gtcgcgccgg gcgctctcgc cggcatccgc
      421 gaccgcccgc tggtgctgaa gcgtttcgtc gacggcgccg atggcgaggc attttatcag
      481 aagcgcgcgc ccgagaagcg gcccgattgg ctgcgcactg tgacgctgtc gttcccatcg
      541 ggccggaccg cggaggagat cgtcgtcgat gatgcagccg ggctctcgtg ggttgtgaac
      601 ctgggctgca tcgagctcca tccgcatccc gttcggtccg gcgatctcga acacccggac
      661 gagctgcgtg tcgatctcga tcccggtccc ggtgtcgcat gggatgatgt gcgccgtgtg
      721 gcgctcgagg taaaagcatt tctcgaggag atggggcttc gcggctggcc caagacgagc
      781 ggctcacgcg gcatgcacgt gaacgtgcgc atcgaaccgc gctggacgtt tgtcgaagtg
      841 cgccgcgccg cgctcgcgct gtcgcgcgcg gtcgagcgcc gtctgccggc gctggcgagc
      901 tcgaagtggt ggaaagagga gcgccacggc gtgttcctcg attacaacca gaatgccaag
      961 gaccgcacga cctgctccgc atactccgtg cgcccgctcc ccgacgcgcg cgtatccacg
     1021 ccgcttcatt ggcacgaagt tcccgactgc gaccccgccg acttcaccgt gctcacggtt
     1081 ccaaagcgct tggcggaaat cggtgatccg cacgcggaga tggattccgc tctggggtcg
     1141 ctcgaaaaac tgctggatct cgcgtcgcaa gacgaggcgg caggtctcgg cgacgcgccc
     1201 tggccgccgc attttcgcaa gatggaaggc gaagcgccgc gagtgcaacc ttcgcgcgcc
     1261 cggtcgaata ggaataaatc cggcaccaag aaaccgcgaa ccaggatgcc actgatcgtg
     1321 gtcgcgaatt cgccaaaccg gcaagcggcg ctcgccggtt tggaaaggtg gaaagctaaa
     1381 catcccgaag cggccgcgca cctcgccgtc gacgatgtgc tgaccgattc catgcgcggc
     1441 cggtcatcga catggacgcg catccgagtg aacctgcgcc atgtgcccga agagctgcgc
     1501 ccgccgcaag aaacgcccga tccggacgac gatcccacgc gcgagtggcg cgagcgaagt
     1561 tgagagtgca gggttgcacg agttgctggt tcaaaacgtt caagccgttc aatcgctgcg
     1621 ctccgttcaa gccccctcct tcatcctccc ccacgtccgt gggggaggaa aagaggtggg
     1681 gggaactttg aacacttaac cttgaaccgc ccgcagaagc gggctagcgg ccgccggcga
     1741 aaatcgtcga cagctcttcc ggcgggacga cttcgagctg gtcataggtg cagtctctgg
     1801 gtttcttgtc ggtgcgccag cgtcgaaatt gcgcggtgtg acgaaagcgg tcgccctgca
     1861 tgtgatcgta ggcgacttca acgaccagtt cgggtcgcag cggctcccac gacaaatcct
     1921 tgccgccgct ccagcggctc tgtgcgcccg gcatccgatg ctcgccatcg tccccctgct
     1981 cggcccaatg tttccacggg tggcgcgcca gagcgttctt gcgatacggc gcgaggaagt
     2041 ccacgagctc gcggcgcttc ttgtccgtga agctcgcgca cacgccgacg tgctgcaaag
     2101 ttccggaatc gtcgtaaagg ccgagcagaa gcgagccgac ggcgctgcgc tcccctctct
     2161 tatgccagcg aaaaccggcc acgacacaat cgcagtcgcg ctcgtgcttg accttgacca
     2221 tcacgcgctt gttgggctcg tagatgccca tcgcctgctt cgcaaccacg ccatccaggc
     2281 ctgcgccttc aaagcggcga aaccaatccg acgctatgct tgaatcgcta gtcgcgggtg
     2341 tcagatgaag cggcggcgtc gaactggcca gcagcgtctc gagcatcccc cggcgttcgt
     2401 ggaacggcac actgcgcaga tctcgacttc cttcgctcag cagatcgaaa aacacgatcg
     2461 acgccggagt ttgtcgtgag agaagcgtga ctctggacgc agcaggatga atgcgcagct
     2521 gcagcgcatc gaagtcgagg gcgccgttct gcgcgatgac gatttcgccg tcgagcacgc
     2581 agcgggccgg caactgggcg cggagcgggt cgagcagttc cggaaaatag cggttcagcg
     2641 atttctcgtc gcggctttgg agcaggacct catcccgagc gcgaaacacc accgcgcgga
     2701 atccatccca tttcggctcg aaaatccagt tctcgccctt cggcaattcg ccggctcgct
     2761 tggcgagcat gggcaagatc ggaggattga cggggaggtt catgatttcg ggcccgttca
     2821 agtcgttcga gtcgtctaag cgccccctcg caagcaacac aatcggttcg cgcaaagatc
     2881 gccaaatcaa ggatccaaac cacacccgga ctttg
//
LOCUS       DASURG010000566         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_197315, whole genome shotgun sequence.
ACCESSION   DASURG010000566 DASURG010000000
VERSION     DASURG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197315"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFY80_10250"
     CDS             complement(<1..223)
                     /locus_tag="VFY80_10250"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881548.1"
                     /note="catalyzes the hydrolytic cleavage of imides that
                     range from linear to heterocyclic and that include
                     hydantoins, dihydropyrimidines, and phthalimides; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX6008336.1"
                     /translation="MSDFDIVIRNGTVATDKDVFESDIGIRGERIAAVGGSLGRGRRE
                     IDARGKLVLPGGVDSHTHIEQISSFGVMCA"
     gene            638..2080
                     /locus_tag="VFY80_10255"
     CDS             638..2080
                     /locus_tag="VFY80_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HEX6008337.1"
                     /translation="MERKQGCDCHQHGPLGTYAEGPTACAPHPKVIASIKKQIARARV
                     LSLADARVAALSGMLGTPLENRTGFNDGVIYPPDVTDTPTSSVLSAARRAQPRISRVH
                     ALATGPQKLNCLVLLVDFSDNRGTKPASHYEKLLFDAANPNSMASFYRELSYGQLTVT
                     GKVVPWIRAPRPYSYYTAGASGTGDYPRNTPGLLEEVLKKWARISGNSFKPFDANRDG
                     YLDGLFLIHAGPGAETLSSPQARKDAIWSHKWTLNTPYKKNGIRAYAYFTAPEDGKLG
                     VFSHEFGHFLGLPDLYDTSYRSHGIGDWCLMAGGSWGGGGSQPTRMSAWCLERLGWVK
                     PRKLTAARKLALETLESSKTQCYRIEAGSVARNEYFLLENRQKKGRDAALPGHGLAVW
                     HIDDTQSSNDNPLAYKVALVQADGRRDLELLRNSGDPADLFPGSKAVTSVSGSSANHP
                     HLRANSGVATSVSLTGIGESRGRLTLKAKP"
     gene            complement(2077..2358)
                     /locus_tag="VFY80_10260"
     CDS             complement(2077..2358)
                     /locus_tag="VFY80_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008338.1"
                     /translation="MLLRPNKSFIEGKVKRVQRSADGVGANVVVDVLECKPAGRFDDF
                     IAAKPGAELTLFIAEPDAIEAGKSYRLTARVLGGPNGERMVIERAERKR"
     gene            complement(2589..3563)
                     /locus_tag="VFY80_10265"
     CDS             complement(2589..3563)
                     /locus_tag="VFY80_10265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008339.1"
                     /translation="MKKEWLALGLCCAAAVSAAEKSDPASAFPAKPIRWVVPFTPGAS
                     TDLIARAVGQRLTEIWGIQIIVDNRPGAGGGIGGEIVARAVADGYTVLLSTSGPNIGN
                     TLLAKKPPYRVEDFAYVAIVADNPMVLVVHPSFPAKSPNEFVEYVKANPGKVNWASAG
                     VNSTPHIAMAVLAGATGIKLTHVPYKGGAPAMIDVVSGQVAGILTSVATAEAAIRQGR
                     VRVIGVAGPKRQPAIPDVPTFAEAGIRNADTPNFYGMAAPAGVPAAIVRKLNAGVNDA
                     LAQPGVRKRLGDLGMEIVGGSPEDATQYVMSQVARVRGLIKAGVLTPE"
     gene            3821..4687
                     /locus_tag="VFY80_10270"
     CDS             3821..4687
                     /locus_tag="VFY80_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008340.1"
                     /translation="MAQVGRKSTFEAGNVRFAVQHELWDGNLDDHSDQGVAILVAGTV
                     GGKEVTMLRFNCFDLERSYVYGPEGVNKLCRMDPTVDGNPIGWSVKQIRTRLPDMLRR
                     AKYEEIAQGIDAQAVGRALPEVQSTARALFASGRNTVKHNRGTEIFEAGNFRFGLELR
                     KQVGGDGGLAIHVLADLAGTPGRTYMEETELLAFDCFREAPHYHYGPRNKNHRIFWDK
                     TLVPDPLEWTLEQFKSGKLRDMIQRAGYPGVAADYDADLVSAVLPALEKRAREIQAQP
                     LPAEPTPNLVPR"
BASE COUNT          932 a         1546 c         1535 g          906 t
ORIGIN      
        1 cggcgcacat cacgccgaac gaggaaatct gctcgatatg cgtgtggctg tcgacgccgc
       61 caggaaggac gagcttcccg cgcgcatcga tctcgcgtcg accccgaccg agcgatcctc
      121 cgaccgccgc gatgcgctca ccacggatgc cgatgtcgct ttcgaacacg tccttgtcgg
      181 ttgcgaccgt gccgttgcga atcacgatgt cgaaatcact catgagcttc ggttcaccag
      241 ggtagattat tgagccgctt gctcgagtat acgaggcatt gcgacgtccg gcaggactgg
      301 ctaggggagg gcgcccccac aatcgactat caggtcgaga gagctttcac gggcgccacg
      361 cgcaatgact cccccggttt ccacgccgag gcgcgagaag ccggcggatc cgcctcgtat
      421 tgccaccgag ctccgagcca attgcggcgg acctccggcg ctcgcctctg agcccttgat
      481 gcagcgacgg tccggatttc ttgctatcgc cttccaacta ccattacgat gccatataag
      541 ctaaagtcat atagcaatga gagcagtgtc gaggaaggtt ggatcagggc ggatattgga
      601 tctcatagac gagatctctg cgcttatggg gggagaaatg gaacgcaagc aaggctgcga
      661 ttgccaccag cacggaccgt tgggaaccta tgccgagggg ccgaccgcct gtgcgccgca
      721 cccgaaagtc atcgcgagca tcaagaagca gatcgcccgg gcgagggttc tttcgctcgc
      781 cgatgcccgg gttgcggcgc tttccgggat gcttgggacg ccgctggaaa accggactgg
      841 cttcaacgac ggcgtcatct atccacctga cgtgacggac acaccgacga gctccgtgct
      901 ctcggctgcg aggagggcgc agccgcgcat ctcgcgcgtc catgccctgg cgacaggtcc
      961 acagaagctc aactgtctgg ttctcctcgt cgacttctcc gacaataggg gaacgaagcc
     1021 ggctagccac tacgagaaat tgctgttcga cgcagccaat ccgaattcga tggcttcgtt
     1081 ctatcgtgag ctttcatatg gtcagcttac ggttaccggc aaggtcgtac cttggatccg
     1141 cgccccgcgg ccgtatagct actacacggc tggcgctagt ggcacgggcg attacccgag
     1201 aaacacgccc ggtctgctcg aggaagttct caagaagtgg gcgaggatct ccggcaatag
     1261 cttcaagccg ttcgacgcta atcgggatgg ctatctcgat ggtctgtttc tcatccacgc
     1321 cggtcccggc gcagaaacgc tttcctctcc gcaagcccgg aaggatgcaa tctggtccca
     1381 taagtggacc ttgaatactc cctacaagaa gaacggaatc agggcatatg cctatttcac
     1441 tgccccggaa gacggaaagc ttggtgtatt cagccacgag ttcggccatt ttctcggcct
     1501 gcctgatctc tacgacacga gctaccggag ccatggcatc ggcgactggt gcctcatggc
     1561 tggtgggtcc tggggcggcg gcgggtcgca accgacccgc atgtcggcgt ggtgcctgga
     1621 gcggcttggc tgggtgaagc ccaggaagct gacggctgcg cgaaagctcg cactcgagac
     1681 gctcgagtcc tccaagacgc aatgctatcg gatcgaggcc ggcagcgtcg cgaggaacga
     1741 atacttcctc ctggaaaacc ggcagaagaa gggccgcgac gcagctctac cgggtcacgg
     1801 cctcgctgtc tggcacatcg acgacacgca atcgagcaac gataacccgc tcgcctacaa
     1861 agttgcgctg gtacaggccg atggacgccg ggatctggag ctcctgagaa attccggcga
     1921 tccggcagac ctctttccgg gatcaaaggc tgtcaccagc gtcagcgggt caagtgccaa
     1981 ccatccgcac ctgcgcgcaa atagcggcgt ggcgacatcc gtctcgctga cgggtatcgg
     2041 cgagagccgc ggccggttaa ctctgaaggc aaaaccctag cgcttgcgct cggcgcgctc
     2101 gatgaccata cgctcgccat tcggcccgcc gagcacccgt gcggtcagcc gataggactt
     2161 gccggcctcg atcgcatccg gctcggcgat gaagagtgtc agctcggcgc cgggcttggc
     2221 agcgatgaaa tcatcgaatc tgccagcagg tttgcattcc agcacatcga ccacgacgtt
     2281 cgcgcccacg ccatcggcgg aacgctgaac gcgcttcacc ttgccttcga taaagctctt
     2341 gttcgggcgg agtaacatgt tttgcatcgc tagccaactg ttccgaccat tgtccgccga
     2401 aaaagtttgg tgtgcgaact cacactccag cgcaggcgtc cggaacctcc gccggagtgc
     2461 gactcgcata ggcatggcgg gagggcgaat ctggcgtatc gatcgggacg cgacgcatac
     2521 ggagcgattc cgaactggaa gccattccct cacctgccgg gcggcgatct ccgcaaaacg
     2581 agcgcgtgct actctggcgt cagcacgccg gctttgatga ggccccgcac gcgcgcgacc
     2641 tgactcatca cgtactgcgt cgcgtcttcg ggcgagccgc cgacgatttc catgccgaga
     2701 tcgccgagac gcttgcgcac gcctggctgc gcgagtgcgt cgttcacgcc tgcgttcagc
     2761 ttgcgcacga tcgccgcagg cacgcctgcc ggcgccgcca tgccgtagaa attcggcgtg
     2821 tccgcgttcc tgatgccggc ttccgcgaat gtcggcacgt ccgggatcgc tggctgacgc
     2881 ttcggtcccg ccacaccgat cacccgcacg cgcccctgcc tgatcgccgc ctcggcggtc
     2941 gcgaccgagg tgaggatgcc ggcgacctgg ccgctcacga cgtcgatcat cgcgggcgcg
     3001 ccgcccttgt agggcacgtg cgtcaacttg atcccggtgg ctccggcgag gactgccatg
     3061 gcgatatgtg gcgtgctgtt cacgcccgcg gaagcccagt tcaccttgcc gggattcgcc
     3121 ttcacatatt ccacgaattc gttcgggctc ttcgccggaa acgacggatg cacgacgagg
     3181 accatcgggt tgtcggccac gatggcgacg taggcgaaat cctcgacacg gtagggcggc
     3241 ttcttcgcga ggagcgtatt gccgatgttc ggcccgctgg tcgagagcag cacggtgtag
     3301 ccgtccgcca ccgcacgcgc gacgatctcg ccgccgatcc cgccgccggc tccggggcgg
     3361 ttgtcgacga tgatctgtat gccccagatc tcggtgagac gctggccgac cgcgcgcgcg
     3421 atgagatcgg tcgatgcgcc gggcgtgaag gggacgaccc agcggatggg cttggcaggg
     3481 aacgcggacg ccgggtccga tttctcggcc gcggagaccg ccgctgcgca gcacaagccc
     3541 aatgcgagcc attccttttt catggtggct attgtgccac gcgcacgggc aatcgcgtcc
     3601 ggcgcgcgcg gaccaagagc gatacgtccg tgcacgaagc cgcttgcaaa gacgccggcg
     3661 gattaccccc acccgtgccc gccgggtaga tcgtgccgtg ctctgtcttt cccttgaaat
     3721 cgcaaggtaa tcctcttcgt gatatggtct tgcttcgttc tggcgcttgg aatagattgc
     3781 ccgggatcga tttttcttga tcgaattggg agatttcgtc atggcgcaag tcggtcgtaa
     3841 gtccacgttt gaagcgggca acgttcgttt cgccgtccag cacgagctgt gggacggaaa
     3901 tctcgacgac cactccgacc aaggtgtggc catcctcgtc gccggaacgg ttggagggaa
     3961 agaggtgaca atgctgcggt tcaactgttt cgatctggag cgcagctacg tgtatggtcc
     4021 cgaaggcgtg aacaagctct gccgcatgga tcctacggtg gacggcaacc cgatcggctg
     4081 gtccgtaaag cagatcagga cccggttgcc ggacatgctc cggagggcga agtacgagga
     4141 gatcgcgcaa gggatcgatg cgcaggcggt cgggcgcgcg ttgcccgagg tgcaatccac
     4201 ggctcgagca ctgttcgcga gcggaagaaa caccgtcaag cacaaccggg gaaccgagat
     4261 cttcgaggct ggcaactttc ggttcggcct ggagctgcgc aagcaggtcg gaggcgacgg
     4321 cggtctggcg atccacgtgc tggccgatct ggcgggcacg ccaggtcgta cctacatgga
     4381 agaaaccgaa ttactcgcct tcgactgctt ccgcgaagcc ccgcactatc actatggtcc
     4441 tcggaacaag aaccaccgca tcttctggga caagacgctg gtgcctgacc cattggagtg
     4501 gacgctggaa caattcaaga gcggcaagct gcgcgacatg atccaacgcg ccggctaccc
     4561 cggcgtggcg gcggactatg acgcggacct ggtgtctgct gtcttgccgg cactggaaaa
     4621 gcgggcgcgt gaaatacaag cccaaccgct ccctgccgaa cccaccccca acctggtgcc
     4681 gcgttaatcg gcgcaggaga cctgaaccgg cgcctggttc tgcgggccgc aggggtcacg
     4741 aaagctctgg aggagtcgct cggcgcaaat tgtgcgggcg gatgcggcgt cgactgcctg
     4801 gcacagcttc ttctcgaggt caccacctcc tcggcgcgtg caccccgcca cagattgtcg
     4861 gcgcgaatct ttgaccaatc gtttcatgcg ggcaggcccg attgcatcgg accggaggc
//
LOCUS       DASURG010000567         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1014633, whole genome shotgun sequence.
ACCESSION   DASURG010000567 DASURG010000000
VERSION     DASURG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014633"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VFY80_10275"
     CDS             <1..1673
                     /locus_tag="VFY80_10275"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6008341.1"
                     /translation="RVHRGFKRSQAKQEAVRLLDLVGIPDAARRVSDFPHQFSGGMRQ
                     RVMIAIALSCNPKLIIADEPTTALDVTIQAQILKLMKDLSRELGIALVVITHNLGIVA
                     RYADRVNVMYAARMAEQGSAAAVFAKPLHPYTAGLLRSVPRLDQPRGRKLETIEGLPP
                     NLLDPPPGCRFAPRCPARLAECVAAPPPLVAVEAHRYSACIRAKEMAEVGATGLGLQS
                     ANPQPPERKAVDTAKALLDVRELRTYFEVTAGLGLFKRSRAEVRAVDGLSFQVFRGET
                     VGLVGESGCGKTTVGRTLLRLEEATSGEMVFAGESVTRAAGEALKRYRRQIQVIFQDP
                     YSSLNPRMTVGDIIAEPMLVYRLASGRRAAHARAVELLTQVGLFEYMAERYPHELSGG
                     QRQRVGIARALAMQPSFIVCDEPVSALDVSIQAQIINLLEELQQKFGLTYLFIAHDLA
                     VVRHISDRVIVMYLGKVMEIADRDTLYADPQHPYTQALLDAVPIPDPALEAKRVSRVL
                     GGEVPSPLNPPRGCVFHTRCPRAIEACKMEVPRLREVRPGHFAACIRL"
     gene            1715..3292
                     /locus_tag="VFY80_10280"
     CDS             1715..3292
                     /locus_tag="VFY80_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6008342.1"
                     /translation="MKSNALLCAALALFAGLACAAQTPKPGGVLKFAVSAEPPNYDCH
                     AQTSFAFIHPVRPHYSTLLEFDTAKYPAIKGDLAESWTVSQDGLTYTFKLRKGVKFHD
                     GSTLTSEDIKATYERLRNPSPGVRSVRQASYADIASIETPDPQTVVFKLKKRNASMLA
                     NFASPWDCVYSAAKLKQDPRFPERNILGTGPFTFAGHVAGSHWTGRKFDDYFRKGQPY
                     LNGYVAIFISGAPMVNALSAGEVLVEFRGQSPADRDRIVKALGDKAVVQEIPWVCSLV
                     VTFNTKKKPFDDARVRRALSLAIDRWGGAQALSKVALVRHVGGILRPGYDLAASEKEL
                     LQMPGFAKDINASRAEARKLLKEAGVPNLTFTLTNRNVAMPYTPVGVFLIDQWRQVGV
                     NVKHEQLETRLYLAAQQRDNPTFDAALDFNCDFMDEPNLQLQKYLSHDRSAINYAQQT
                     DRTLDELYDKQSGELDKKKRYTILREFEKRALEQSYTIPTIWWHRIIVQHRPLKGWHI
                     TPSHYVGQDLAEVWLDQ"
     gene            complement(3322..>3503)
                     /locus_tag="VFY80_10285"
     CDS             complement(3322..>3503)
                     /locus_tag="VFY80_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008343.1"
                     /translation="DLSLRREDERRHSKALPTVDWRREHLHSTALPPTECKPNIVIPA
                     YAGIQSQFGVLAFPE"
BASE COUNT          641 a         1140 c         1110 g          612 t
ORIGIN      
        1 tccgggtgca ccggggcttc aaacggtccc aggccaagca ggaggccgtc cggctgctcg
       61 acctggtcgg gatcccggac gcggcgcggc gggtcagcga cttcccgcac cagttctccg
      121 gcggcatgcg ccagcgcgtc atgatcgcga tcgcgctctc ctgcaatccg aagctcatca
      181 ttgccgatga gcccacaacc gccctcgatg tcacgatcca ggcgcagatc ctcaagctca
      241 tgaaggatct ctcgcgcgag ctcggcatcg cgctcgtggt gatcacgcac aacctcggca
      301 tcgtcgcccg ctatgccgac cgcgtgaatg tgatgtatgc ggcgcgcatg gcggagcagg
      361 gcagcgccgc agccgtattt gcgaagccgc tccaccccta caccgcggga ctactccgtt
      421 cggtgccgcg cctcgatcag ccgcgcggac gcaagctcga aacgatcgaa ggtcttcctc
      481 cgaacctgct cgacccgccg cccggctgcc gttttgcccc gcgttgtccc gcgcggctcg
      541 cggagtgcgt cgccgcaccg ccaccgctcg tcgccgtcga ggcccatcgc tactccgcgt
      601 gcatacgcgc gaaggaaatg gcggaggtcg gcgcaaccgg gcttgggctc cagagcgcca
      661 atccccagcc gcccgagcga aaggcggtcg ataccgcgaa ggcgctgctc gatgtgcggg
      721 agctgcgcac ttatttcgag gtgaccgcgg ggctcggact gttcaagcgc tcccgcgccg
      781 aggtgcgcgc ggtggacggc ctctcgttcc aggtattccg aggcgagacc gtgggactgg
      841 tgggcgaatc gggctgcggc aagacgactg tgggccgcac gctgctgcgc ctcgaagaag
      901 cgacctcggg cgaaatggtt tttgcgggcg aaagcgtgac gcgcgcggcg ggtgaagcgc
      961 tgaagcgtta ccgccggcag atccaggtga tcttccagga cccgtacagc tcgctcaacc
     1021 cgcgcatgac ggtgggggac atcatcgccg agccgatgct cgtataccgg ctcgcgagcg
     1081 gccgcagggc ggcgcacgcc cgggcggtcg agctgttgac gcaggtcggt cttttcgaat
     1141 acatggcgga gcgttatccg cacgagcttt ccggcgggca gcgccaacgc gtgggcatcg
     1201 cgcgcgcgct cgcgatgcag ccctctttca tcgtctgcga cgagccggtg tccgcgctcg
     1261 acgtgtcgat ccaggcgcag atcatcaatc tcctggaaga gttgcagcag aagttcggcc
     1321 tcacgtatct tttcatcgcg catgacctcg ccgtggtgcg gcacatctcg gatcgcgtga
     1381 tcgtgatgta tctcggcaag gtgatggaga tcgccgatcg cgatacgctc tacgccgatc
     1441 cgcagcatcc ctacactcag gcgctgctcg acgcggtgcc cattccggat cccgcgctcg
     1501 aggcgaagcg tgtgtcgcgg gtgctcggcg gagaagtgcc gagcccgctc aacccgccgc
     1561 gcggctgcgt cttccacacg cgctgcccgc gggcgatcga agcgtgtaag atggaggttc
     1621 cccgattgcg ggaagtgcgg cccgggcatt tcgcggcctg catccggctc tagttccgaa
     1681 gtgacaagtc cagaaaacag agagaggagg gttcatgaaa tcgaatgcat tgttgtgcgc
     1741 cgcgttggcg ctttttgccg gccttgcgtg tgccgcgcag acgcccaagc cggggggagt
     1801 gctcaagttt gccgtaagcg ccgagccgcc caactacgac tgccatgcgc agacatcgtt
     1861 tgccttcatc cacccggtcc gtcctcacta ctccacgctg ctcgagttcg acaccgcgaa
     1921 atatccggcg ataaaaggcg acctcgccga atcatggacc gtctcgcagg acgggctcac
     1981 ctacactttc aagctgcgca agggcgtcaa gtttcatgac ggctcgacgc tcacctccga
     2041 ggatatcaag gcgacctacg agcgcctgag gaatccgtcc cccggcgtgc gttcggtgcg
     2101 ccaggcgagc tacgcggaca tcgcgtcaat cgagactccc gacccgcaga cggtcgtgtt
     2161 caagctcaag aagcgcaacg cctcgatgct cgccaacttc gcgagtccgt gggactgcgt
     2221 gtacagcgcg gcgaagctga agcaggatcc gcgctttccc gagcgcaaca ttctcggcac
     2281 cgggccgttc acgtttgcgg gccacgttgc gggctcacac tggacgggac gcaagttcga
     2341 cgactacttc agaaagggcc aaccgtatct caacggctat gtcgcgatct tcatcagcgg
     2401 cgcgcccatg gtgaacgcgc tttccgcggg cgaggtcctg gtcgagtttc gcgggcagtc
     2461 gccggcggat cgcgaccgca tcgtgaaggc cctgggtgac aaggcggtgg tgcaggagat
     2521 accgtgggtc tgctcgctgg tcgtcacctt caataccaag aagaagccgt tcgatgatgc
     2581 gcgcgtgcgc cgcgcgctgt cgctcgcgat cgaccgctgg ggcggcgcgc aggcgctgtc
     2641 caaggtcgcg ctggtgcgcc atgtgggggg catcctgcgt cccgggtacg accttgcggc
     2701 atccgagaag gaactccttc agatgccggg cttcgccaag gacatcaacg cttcccgcgc
     2761 cgaagcgcgc aagctcctga aggaagccgg tgtcccgaat ctcacgttca cgctcaccaa
     2821 ccgcaacgtc gcgatgccct atacaccggt cggcgtattc ctgatcgacc agtggcgaca
     2881 ggtcggcgtc aacgtgaagc atgagcagct cgaaacgcgg ctctatctcg ccgcgcagca
     2941 gcgcgacaat ccgacgttcg atgccgcgct cgatttcaat tgcgacttca tggacgagcc
     3001 caaccttcag ctccagaagt atctgtcgca cgaccgctcg gcgatcaact acgcgcagca
     3061 gaccgatcgc acgctggacg agctctacga caagcagtcg ggcgaactgg acaagaaaaa
     3121 gcgctatacc atcctgcgcg agttcgagaa gcgcgcgctc gagcagtcgt acaccatacc
     3181 caccatctgg tggcaccgga tcatcgtgca gcaccggccg ctgaaaggct ggcacatcac
     3241 gccgagccac tacgtgggtc aggatctggc cgaggtttgg ctcgatcagt aaggtgacgc
     3301 caaaagaacg caagaaatgc gtcattccgg gaaagccaga accccgaact gtgactggat
     3361 tccggcgtac gccggaatga cgatgtttgg tttgcattct gtcggcggca aggcggtgga
     3421 atgaagatgt tcgcgtctcc aatcgactgt cggcaacgcc ttggaatgac gacgttcgtc
     3481 ttcccggcgc aagctgagat cca
//
LOCUS       DASURG010000568         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_503927, whole genome shotgun sequence.
ACCESSION   DASURG010000568 DASURG010000000
VERSION     DASURG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503927"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1046)
                     /locus_tag="VFY80_10290"
     CDS             complement(63..1046)
                     /locus_tag="VFY80_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6008344.1"
                     /translation="MKPIEISFTPVDNQRLANLCGALDENLRQIETALDVSIARRGER
                     FALSGKPQQTKVAARVLQDFYRKADQTLTLEDVQLGLIETANPRQARTASDETPALIT
                     RRQDLHGRTPRQVQYLQNILTHDVTFSIGPAGTGKTYLAVACAVDAFERDSVRRIVLV
                     RPAVEAGERLGFLPGDMAQKVDPYLRPLYDALYDLMGFDKVARMFERGTIEIAPLAYM
                     RGRTLNHSFVILDEAQNTTPEQMKMFLTRIGFGSKAVVTGDITQIDLARGQKSGLVEA
                     RDVLNRVRGIAFTYFESEDVVRHPLVQRIVAAYESYKPRATPRVSARDEKA"
     gene            complement(1043..2377)
                     /gene="miaB"
                     /locus_tag="VFY80_10295"
     CDS             complement(1043..2377)
                     /gene="miaB"
                     /locus_tag="VFY80_10295"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX6008345.1"
                     /translation="MSRKFYIKTFGCQMNEYDSAKLADVLFETEGMEPTDDPASADLL
                     LFNTCSVREKAQEKVFHDLGRVKHFKRARPDVLIGVGGCVASQEGAAIVARAPYVDLV
                     FGPQTLHRLPQMMEARRASGKPQVDISFPEIEKFDALPPARVEGPTAFVSIMEGCSKY
                     CTFCVVPYTRGEEVSRPLDDVLTEVAELAAQGVKEITLLGQNVNAYRGQMEGGEHADF
                     ALLLEYVAEVPGIQRLRYTTSHPREFTQRLIDVYAALPKLVSHVHLPVQSGSDRVLAA
                     MKRGYTVLEYKSIVRRLRERRPDISISSDFIVGFPGETEADFAATMKLIDEVGFDASF
                     SFIYSPRPGTPAAGLPDATPHQEKLRRLQTLQRRIEEHAQSISQAMVGTRQQVLVDGL
                     AKKNATELSGRTANNRIVNFRGPRECIGRLVEVAVTAALPHSLRGEACARAH"
     gene            complement(2396..2725)
                     /locus_tag="VFY80_10300"
     CDS             complement(2396..2725)
                     /locus_tag="VFY80_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008346.1"
                     /translation="MHRLIAAVALAFAMMATPAAAQLPRPLPANGKLGHLAGQLQYPL
                     VEIDRKTLRLAPGGVIVDQNNRFIVHGALPANADVLYVLDGRGDISRIILLTSPELAR
                     IQAAQKR"
     gene            complement(2817..2892)
                     /locus_tag="VFY80_10305"
     tRNA            complement(2817..2892)
                     /locus_tag="VFY80_10305"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2857..2859),aa:Met,seq:cat)
     gene            2995..>3559
                     /locus_tag="VFY80_10310"
     CDS             2995..>3559
                     /locus_tag="VFY80_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6008347.1"
                     /translation="MAAPYDAVIVGAGLVGASLALALERARMRVAVVEPQAPSDPSVD
                     GSWDARVYAISPGNAAFLENCGAWLRLPPERISRIEAMRVYGDAPGAQLEFSAYDAGL
                     RELAFIVENHRLQHALWACLGSAPHVTLLAPARCARLAWEPDVVTLTLEDGTALRSTL
                     IIGADGADSWVRREAGIAAKPVSYGELG"
BASE COUNT          612 a         1094 c         1162 g          691 t
ORIGIN      
        1 ccgcgccgag acggcgtact gcacggtcaa ttggaggcgt ggcgcacgcc ttctgcgccg
       61 cttcatgctt tctcgtcccg ggcgctaaca cgcggtgtgg cacgcggctt gtagctctca
      121 taggcggcga cgatgcgctg caccagcgga tggcgcacga catcctcgct ttcaaaataa
      181 gtgaacgcaa tgccgcgcac ccgattgagc acgtcccgcg cctcgacgag tccgctcttc
      241 tgcccgcggg ccagatcgat ctgcgtgata tcgcccgtga cgacggcctt gctgccgaag
      301 ccgatgcgcg tcagaaacat tttcatctgc tcgggtgtcg tattttgcgc ttcgtccagg
      361 atcacgaacg agtggttgag cgtgcgcccg cgcatgtagg cgagcggggc aatttcgatc
      421 gtgccgcgct cgaacatccg cgcaaccttg tcaaaaccca tgaggtcgta gagcgcgtcg
      481 tagagcgggc gcaggtaggg gtccaccttt tgcgccatgt cgcccgggag gaagcccagc
      541 cgctcgccgg cctcgactgc agggcgaacg aggacgatgc gcctcacgct gtcacgctcg
      601 aatgcgtcca ccgcacaggc gacggcgaga tacgtctttc ccgtgccggc cggaccgata
      661 ctgaaggtca cgtcgtgcgt gaggatgttc tgcagatact gcacttgccg cggggtgcgg
      721 ccgtgcaggt cctgacggcg agtgatgagc gccggcgttt catcggacgc cgtgcgcgct
      781 tgcctgggat tcgcagtctc gatcagccca agctggacgt cttccagagt gagcgtctgg
      841 tcggccttac ggtaaaagtc ctgcagcacg cgcgcggcga ctttcgtctg ctgcggcttg
      901 ccgctcagcg caaagcgctc gccgcgacgt gcgatgctca cgtcgagcgc cgtttcgatc
      961 tgacgcaggt tttcatcgag cgcgccgcac agattggcga gccgctggtt gtccaccggc
     1021 gtgaaggaaa tctcgatggg tttcaatggg cgcgcgcgca cgcctccccg cgcagcgaat
     1081 gcgggagcgc tgcggtgact gcgacttcga cgagccggcc gatgcattcc cgcgggccgc
     1141 gaaaattgac gatccggttg ttcgcggtgc ggccggaaag ctcggtggcg ttcttcttcg
     1201 cgaggccgtc gacgagcacc tgctggcgcg tgcccaccat ggcttggctg atcgactgcg
     1261 cgtgctcttc gatccggcgc tggagcgtct gtagccgccg gagcttttcc tggtgcggcg
     1321 tggcgtccgg cagaccggca gccggcgtgc cgggccgcgg gctgtagatg aagctgaagc
     1381 tcgcatcgaa tccgacctcg tcgatcagtt tcatcgtggc tgcaaagtca gcctcggtct
     1441 cgcccgggaa gccgacgatg aaatcggacg agatcgaaat atcgggccgc cgctcgcgca
     1501 atcgccgcac gatcgacttg tactcaagca ccgtataacc gcgtttcatc gctgcgagca
     1561 cacggtcgga gcccgactgc accgggagat gaacgtgact cacgagcttg ggaagcgcgg
     1621 cataaacatc gatcaggcgc tgtgtgaatt cgcgcgggtg cgatgtcgta taccgcaggc
     1681 gctggatccc cggaacttcc gctacgtact ccagaagaag agcgaaatcc gcgtgctcgc
     1741 ccccttccat ttgaccgcgg tatgcattca cgttctggcc gaggagcgtg atttccttga
     1801 cgccctgcgc cgcgagctct gcgacttccg tcagcacgtc atcgagggga cgggagactt
     1861 cttctccgcg cgtatacggc acgacgcaga aggtgcagta cttgctgcag ccttccatga
     1921 tcgacacaaa agccgtcggg ccttcgacgc gggcgggcgg aagggcgtcg aacttctcga
     1981 tttcggggaa cgatatatcg acttgcggct tgccgctcgc gcgccgcgcc tccatcatct
     2041 gcggcaggcg gtgcagcgtc tgcggaccga agaccaagtc gacgtatggg gcgcgcgcga
     2101 cgatcgcggc cccttcctgg ctcgcgacgc aaccgcctac cccgatcagc acgtcggggc
     2161 gggcgcgctt gaaatgcttc acgcggccga gatcgtggaa cactttctcc tgggctttct
     2221 cgcgcaccga gcacgtgttg aagagaagga gatcggcgct tgccggatca tcagtgggct
     2281 ccatgccctc tgtttcgaac agcacgtcgg cgagcttcgc cgagtcgtac tcgttcatct
     2341 ggcagccgaa cgtcttgatg tagaacttcc tggacacggc cttgcggacg ccgtctcagc
     2401 gtttctgcgc ggcttggata cgcgcgagtt ccggcgaggt gaggagaatg atgcgtgaga
     2461 tgtcgccacg gccgtccaga acgtagagca cgtccgcgtt cgcgggcagc gcgccatgca
     2521 cgatgaaccg gttgttctga tcgacgatca cgccgccagg cgcaaggcgc agcgtcttgc
     2581 ggtcgatttc caccagagga tactgaagct gtcccgcgag atgcccgagc ttgccgtttg
     2641 ccggcagcgg tcgcggcagt tgggcagcgg caggggtcgc catcatcgcg aacgcgagcg
     2701 caaccgccgc gatcagtctg tgcatggaat cacgatatca caacgtaatc aattacttca
     2761 accaagcgtt cccgatactg agttcgattg gcgcatcgtg atcgttcaat gtggattggt
     2821 ggcgagtcag ggatttgaac cccggaccaa cggattatga ttccgctgct ctaaccgctg
     2881 agctaactcg ccacggaaag cggcggagtc taaagacggg gcctcgcatt gtcaagccaa
     2941 agcaggcgat aattcattgt tatttcagtc acattgatgc ttgcaggagg gtgcgtggcg
     3001 gcgccatacg acgcggtgat cgtgggagcg ggcttggtgg gtgcgagcct cgctctcgcg
     3061 ctggagcggg cgcggatgcg cgtagcggtt gtcgaaccgc aagcgccgtc tgacccgtct
     3121 gtggacggga gctgggatgc gcgcgtctac gccatcagcc cgggcaacgc cgcatttctc
     3181 gagaactgcg gcgcgtggtt gcgcctgccc cccgagcgca tatcccgtat cgaagccatg
     3241 cgggtttacg gtgatgcgcc cggtgctcag ctcgagttca gtgcatacga tgcgggcttg
     3301 cgcgagcttg cattcatcgt agagaaccac cgtctgcagc acgcgctgtg ggcttgcctc
     3361 ggttccgctc cgcacgtgac gctccttgca cccgcgcgct gtgcgcgcct cgcgtgggaa
     3421 ccggatgtcg tcacgttgac gctggaggac ggcacggcgc tcagaagcac tcttatcatt
     3481 ggcgcggatg gcgctgactc ctgggtaaga cgggaggctg gaattgcggc gaagcctgtt
     3541 tcgtacggcg agcttggcg
//
LOCUS       DASURG010000569         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_606753, whole genome shotgun sequence.
ACCESSION   DASURG010000569 DASURG010000000
VERSION     DASURG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606753"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFY80_10315"
     CDS             complement(<1..186)
                     /locus_tag="VFY80_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6008348.1"
                     /translation="MGMHSFKASDGLGLAYALDDYTDPWRAADTVILLHAAMGSSRRF
                     YAWVPHLAGEFRVVRLDL"
     gene            complement(198..1025)
                     /locus_tag="VFY80_10320"
     CDS             complement(198..1025)
                     /locus_tag="VFY80_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6008349.1"
                     /translation="MPLSSETHYVTASDGLELAYAIDDFSDPWKPREALILIHAAMGS
                     SRRLYKWVPILAREFCVVRPDMRGHGATGMPGPGQLSLERLTRDVIELADHLQLQRFH
                     IAGSSAGAIVAMRTTLDFPERIVSLTNFASTPGLKNSLIDMDKWVSHIRARGMRGFLE
                     DTIAERFPNVADKGFLAWFIEESARTDPELFARFGPMMKAVDFTDRLHEIKCPMLNVV
                     PGHDPLGAPGQYEVYKTHVPHCEFIVYEGLPHNITDSVPERCANDLRDFLRRHRQVR"
     gene            1203..2027
                     /locus_tag="VFY80_10325"
     CDS             1203..2027
                     /locus_tag="VFY80_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6008350.1"
                     /translation="MHSRNARRVATESLAAHRAPCAWKSTAITASSILTGVAIWALAS
                     HGLHSPLFPSPLAIVRAGGGMLLDGSLVKDAAVSLQRIGIGFGIGCAVGIPLGLAMGL
                     YRPVRVFFDPYVQFLRFVPPIAWLIPAIMWFGIGEVSKVLIIFDMTVFLVLLNTIAGV
                     AAVRRNHVRSALCFGAGRWQIFAWVIFPAAMPYIVAGARIAMGNSFAAVVGAELIAAN
                     AGLGYLIVDSGTWLAMDNMFAAMLTLALLGIAADRLFRSLTGRLLRPYLAHVTDIR"
     gene            2008..2856
                     /locus_tag="VFY80_10330"
     CDS             2008..2856
                     /locus_tag="VFY80_10330"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6008351.1"
                     /translation="MSPISGNSADALRSTSADAGAGRVELAGVDFRFAGSERAVISGL
                     DMTIASGEFFVLLGPSGCGKSTVLNLVAGFEYPTRGEVRAGGRAVRGAGRDRVVIFQD
                     SDSLYHWLSVLDNVAFPLRVQGMRPAERRERALDALKLVGLQDHAHKYPSELSGGMKQ
                     RTQLARALVLESPILLMDEPFAALDAQTRAILQDELVQLGMRVRRTIFFITHDIGEAI
                     LLGDRVAIMRAGPESTIKETIEVGLPRPRRRSGAEFGELYERISRSISDEVRSALRDE
                     ASRPRS"
     gene            2853..3623
                     /locus_tag="VFY80_10335"
     CDS             2853..3623
                     /locus_tag="VFY80_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6008352.1"
                     /translation="MSTRASRRGLAVLGYVASFATLLALWQYFSAKAIIPLLFPSPAS
                     TYRVALKLFEEGRLVADVVASLRRIAIGFTLGSLVGIGLGVLMGSFRTVRLVLEPYLH
                     FFRFLPALAWIPAAMLWLGTGEESKIALLVYTTVFIVALNTMIGVGSVPRDQIRAALC
                     FGASPWQVFRMVRFPATVSHMLTGMRLAMGTCFSTIVAAELLAPEAGLGYLIFSARTW
                     MQTEVAFVGIVLLGLLGFAADRLFQLATTRLFARYRPR"
     gene            complement(3651..4586)
                     /locus_tag="VFY80_10340"
     CDS             complement(3651..4586)
                     /locus_tag="VFY80_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6008353.1"
                     /translation="MAAAAPAASTPATVTVGVAPSPDMSLLIIAVKKDFLKNQGLDAK
                     LEVFESSPSALQGVVAGRADITANTEPPQLAARARGGKIVQVMTGYMSGRQNGLVVNG
                     KLIGKPADFAGKAIGVQRGSGGNYHMVWFLERNKIPLDKVEPKYMAAPDQIAALARGD
                     IAAFFSWEPFLSKAASDVPGAKIFSRSMDDGLSFAGNVVMREDLAKNNKPVAVKIVKG
                     LIAAADWMTANVAEAAKVANEVLRAPSDELVATQLQNLQWPGTLKKSMVEQEIRIAEW
                     GAGIKLFPTSDPRKLVQDFVYPALIKEAAPGRTDL"
     gene            complement(4685..>5116)
                     /locus_tag="VFY80_10345"
     CDS             complement(4685..>5116)
                     /locus_tag="VFY80_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008354.1"
                     /translation="DEGVPQSLDMVAYEMKWPYVDYIERWGDKVGERIRKLIARAEEL
                     TPSDYERLLENRARIRAQVRDVARKADAFVALAASSVAPLGHEFTGSRTYLAYWSWTG
                     FPAFSLPLMRVDGLPLGLQLMGVGDEDGALAAAANWLEQAR"
BASE COUNT          790 a         1723 c         1680 g          923 t
ORIGIN      
        1 tagatcgagg cgcaccacgc gaaattcgcc ggcgaggtgt ggcacccagg cgtagaagcg
       61 cctggagctg cccatcgcgg cgtggagcag gatgaccgta tcggccgcgc gccacggatc
      121 ggtgtagtcg tcgagcgcat aggcgagccc gagaccgtcg gaagccttga aactgtgcat
      181 gcccatcgcg cgcccgctca gcgcacctgg cggtgcctgc gcaggaagtc gcgcaggtcg
      241 ttcgcacacc gctcgggcac cgaatcggtg atgttgtggg gaaggccctc gtagacgatg
      301 aactcgcagt ggggcacgtg cgtcttgtac acctcgtact gccccggcgc accgagcgga
      361 tcgtgcccgg gaacgacgtt cagcatcgga catttgatct cgtgcagccg gtcggtgaaa
      421 tcgaccgcct tcatcatcgg gccgaagcgc gcgaacagct ccgggtccgt gcgcgcggat
      481 tcttcgatga accaggcgag gaaccccttg tcggcaacgt tgggaaagcg ctccgcgatc
      541 gtatcctcga gaaagccgcg catgccgcgg gcgcggatat ggctcaccca tttgtccatg
      601 tcgatgagcg aattcttgag ccccggcgtg gacgcgaagt tggtgagcga gacgatgcgc
      661 tccggaaagt cgagcgtggt gcgcatggcg acgatcgcac cggcggacga gccggcgatg
      721 tggaaccgct gcagttgcag gtgatccgca agctcgatga cgtcgcgcgt gaggcgctcg
      781 agcgagagct gacccggacc cggcatcccg gtggcgccgt ggccgcgcat gtcggggcgc
      841 acgacacaga attcgcgcgc gaggatcggc acccacttgt acaaccgccg cgagctgccc
      901 atcgcggcat ggatcagaat cagcgcctcc cgcggcttcc agggatcgct gaaatcgtcg
      961 atggcatagg cgagctcgag cccgtcgctc gcagtcacgt agtgcgtttc cgagctgagc
     1021 ggcatgatga cgatcctccc tcgctttctg tcgatgctat gctccctggc attatgttgt
     1081 ggcaggcgac agggagcaag gcgatgtctg cagccggatg gaagaccctc gtcctcgaag
     1141 cgagcgttgc gaggcgggca gcgctggaca gggccggcag catccgcggt tgaggcaacg
     1201 cgatgcacag tcgaaatgcg cgccgggtcg cgacggagag cctcgccgcg caccgggcgc
     1261 cctgcgcgtg gaagagcacg gcgatcaccg cgagctcgat cctgaccggc gtcgcgatct
     1321 gggcgctcgc cagccacggc ctgcattccc cgctgtttcc atcgccgctc gccatcgtgc
     1381 gagccggcgg cgggatgctg ctcgacggct cgctcgtgaa ggatgccgcg gtgagcctgc
     1441 aacgcattgg catcggcttc gggatcggct gcgccgtggg tatccccctc gggctcgcga
     1501 tgggtctcta ccgacccgtg cgcgtcttct tcgatcccta cgtgcagttc ctacgcttcg
     1561 tgccgccgat tgcctggctc attccggcca tcatgtggtt cggcataggg gaagtctcca
     1621 aggtcctgat catcttcgac atgaccgtct tcctcgtgct tctgaacacc atcgccggtg
     1681 tcgccgcggt gcggcgcaat cacgtccgca gcgctctatg cttcggcgcc ggccggtggc
     1741 agatcttcgc ctgggtcatc tttccggcgg cgatgcctta tatcgtcgcg ggcgcccgca
     1801 tcgccatggg caactcgttc gccgccgtcg tcggggccga gctgattgcg gccaacgcgg
     1861 gcctgggcta tctcatcgtc gattccggaa cgtggctcgc gatggacaac atgttcgccg
     1921 cgatgctgac cctggccctg ctggggatcg cggccgatcg cctgtttcgc agcctgaccg
     1981 ggcggctgct gcgtccttac ctcgctcatg tcaccgatat ccggtaatag cgccgacgcg
     2041 ttgcgttcca cgagcgccga cgccggcgcc ggcagggtcg agcttgccgg ggtcgacttc
     2101 cgcttcgccg gcagcgagcg agccgtcatc tcgggtctgg acatgacgat cgcgtcgggc
     2161 gagttcttcg tcctgctcgg accctcgggc tgcggcaagt cgaccgtgct caacctcgtg
     2221 gcgggcttcg aatatccgac acgcggtgaa gtgcgcgcag gcgggcgcgc ggtccgaggc
     2281 gccgggcgcg accgggtcgt gatcttccag gacagcgatt cgctctatca ctggctcagt
     2341 gtcctcgaca acgtggcatt tccgctgcgt gtgcagggga tgcgtcccgc cgagcgccgc
     2401 gagcgcgccc tggacgccct gaagctggta gggctgcagg atcacgcgca caagtatccg
     2461 agcgagctct cgggcggcat gaagcagcgc acgcagctcg cccgcgcgct ggtgctcgag
     2521 tccccgatcc tcctcatgga cgagccgttc gccgcactgg atgcgcagac gcgcgccatc
     2581 ctgcaggacg agctggtcca gctcgggatg cgcgtgcggc gcacgatctt cttcatcacg
     2641 cacgacatcg gcgaagcgat cctcctcggc gaccgggtcg cgatcatgcg ggccggtccg
     2701 gaatccacga tcaaggaaac catcgaggtc ggattgccgc gcccgcggcg acgcagtggc
     2761 gccgagttcg gcgagctcta cgaacgcatc agccgcagca tctccgacga ggtgcgcagc
     2821 gcgttgcggg acgaggcgag ccggccgcgg tcatgagcac acgcgcttcg cgccgcggcc
     2881 tggcggtgct cggctacgtc gcctcgttcg cgacgctgct cgccctgtgg cagtacttca
     2941 gcgcaaaggc gatcattccc ttgctcttcc cctccccggc gagcacctac cgcgtggcgc
     3001 tgaagctgtt cgaggagggc cggctggtcg cggatgtcgt cgcaagcctg cgccggatcg
     3061 cgatcggctt cacgctgggg agcctcgtgg gcatcgggct cggcgttctg atgggatcgt
     3121 tccgcaccgt gcgcctcgtc ctggagccct atctgcactt cttccgcttc ctgcccgcgc
     3181 tcgcgtggat cccggccgcg atgctgtggc tgggaaccgg tgaggaatcg aagatcgcgc
     3241 ttctcgtcta taccacggta ttcatcgtgg ccctgaacac catgatcggc gtcggcagcg
     3301 tcccccggga ccagatacgc gccgccctgt gcttcggggc gagcccatgg caggtgttcc
     3361 gcatggttcg attccccgcg acggtgagcc atatgctgac tgggatgcgg ctcgcgatgg
     3421 gcacctgctt ttccaccatc gtcgcggccg agctgcttgc gccggaggcc ggcctgggct
     3481 acctgatctt cagcgcgcgc acctggatgc agaccgaagt cgcattcgtc ggcatcgtgc
     3541 tgctggggct gctcggattc gcggccgacc ggctctttca gcttgcgacc acgcggctct
     3601 tcgcccgcta tcgcccgcgc tgaagcaggc attgcattcg ggtcgtcctg ctagagatcc
     3661 gtgcgcccgg gtgctgcctc cttgatgagc gccggataga cgaagtcctg caccagcttc
     3721 ctgggatcgc tggtcggaaa gagcttgatc ccggcccccc actcggcgat gcggatctcc
     3781 tgctccacca tgctcttctt gagcgtgcct ggccattgca ggttttgcag ctgcgtcgcc
     3841 acgagctcgt ccgagggcgc gcgcaggact tcgttcgcca ccttcgccgc ctccgcgaca
     3901 ttggccgtca tccagtcggc ggcggcaatc aggcccttga cgattttgac ggctaccggc
     3961 ttgttgttct tcgccagatc ctcgcgcatc acgacattgc cggcgaacga gagtccatcg
     4021 tccatcgagc ggctgaatat tttcgcgccg gggacgtcgc tcgccgcctt gctcaggaaa
     4081 ggctcccagg agaagaaggc ggcaatgtcg ccgcgggcga gcgcagcgat ctgatccggc
     4141 gccgccatgt acttgggctc caccttgtcg agcgggatct tgttgcgctc gaggaaccag
     4201 accatgtgat agttgccgcc gctgccgcgc tgcacgccga tcgccttgcc ggcgaaatcg
     4261 gcgggcttgc cgatgagctt gccgttgacg accagcccat tctgacggcc gctcatatag
     4321 ccggtcatga cctgcacgat cttgccgccg cgagcgcggg cagcaagctg cggcggctcg
     4381 gtgttcgccg tgatgtccgc gcgccctgcc acgaccccct gcagcgcgga aggcgacgac
     4441 tcgaacacct cgagcttcgc gtccaggccc tggttcttca ggaaatcctt tttcacggcg
     4501 atgatgagaa gggacatgtc cgggcttggc gccacgccca cggtgaccgt ggcgggcgtg
     4561 ctggcggcgg gcgccgctgc ggccattgcg aacgcgacaa caacacaagc catcctcgca
     4621 atccacccga acatgctgcg ctcccggaaa gttgtcgtga agtcgcggca gcgcgccgaa
     4681 cgcctcagcg cgcctgctcc agccagttgg cggccgcggc cagcgcgccg tcctcgtcgc
     4741 cgacacccat gagctgcaag cccaacggca ggccgtccac gcgcatcagg ggcagcgaga
     4801 aagccggaaa acctgtccac gaccagtatg cgaggtaggt gcggctgccg gtgaactcgt
     4861 gccccagcgg agcgacgctc gatgccgcaa gcgcgacgaa cgcatccgcc ttgcgcgcaa
     4921 cgtcgcgcac ctgcgcgcgg atgcgcgccc ggttctccag cagccgctcg taatccgaag
     4981 gcgtaagctc ctccgcgcgc gcgatcagtt tgcgaatgcg ctcgcccacc ttgtcgcccc
     5041 agcgctcgat gtagtcgaca tacggccatt tcatctcgta agcgaccata tcgagcgatt
     5101 gcggcacgcc ttcgtc
//
LOCUS       DASURG010000570         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_912792, whole genome shotgun sequence.
ACCESSION   DASURG010000570 DASURG010000000
VERSION     DASURG010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912792"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1168
                     /locus_tag="VFY80_10350"
     CDS             257..1168
                     /locus_tag="VFY80_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008355.1"
                     /translation="MSNLDARIAETETAAAQFEPLPEEERAEPRIAAPRRILWRDHLR
                     IGLGSRWLGIAGYRRGLRPALDYKEVITFEQGEVESPPWQAAVDALRVALAGTDLRRP
                     AVTVVLSNHFARYAVLPWNAALRSEVEWLALARHRFSSVHGTAAEQWQIRIAPAGRER
                     PRVACAIDAVLLEALKNAVNGHGSLISAQPYLMAAYNRLQPVIGQSSCWLVIEEPGRL
                     TLALIHDGTWRAIRSRRKDEAWRMTLPRLLDRESALIGLDVPCTEVAVCTHVPFDEEM
                     HGAYRLRDLTFPPGAAAADRELAMALE"
     gene            1174..1701
                     /locus_tag="VFY80_10355"
     CDS             1174..1701
                     /locus_tag="VFY80_10355"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX6008356.1"
                     /translation="MSKLDLDFMRMRPASPRLRWALFAIALAFAADLGISFYSVRHRI
                     AQNEENLAELKRGADGAANAALAMRAPSPEEIRVARDTVQRLAMPWDSLFRALESTAS
                     DKVALLAIDPEAKSGTVVISGEATDYGAALDYVARLGRSGALDRPHLVRHEIARDEAQ
                     NPVKFSISANWSEAK"
     gene            1698..2237
                     /locus_tag="VFY80_10360"
     CDS             1698..2237
                     /locus_tag="VFY80_10360"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEX6008357.1"
                     /translation="MSAAGFLRRVCHELGTMGLASIAIIAGAILFLFTMLKPLEARNV
                     ELQHEIALNARQNASTDANLVRTSTPAAKMAAFYHFLSTDRPMTEWLGRLYAAGQAAG
                     VELQSADYRMQKTGTRIERYQIRIPLRGNYAQIRGFLDNALVDIPVLSLDEVKFKRER
                     ASDAQIEAELLLSLHLVNP"
BASE COUNT          400 a          744 c          731 g          396 t
ORIGIN      
        1 caaccgcgtg acgttcgtca cgtaggagaa cgatatcgct ttgacggcaa ctgaatcgga
       61 gaagtcacaa tgaagcagtc cctgcgtgca gcgactgcgc ttgccgcttt tgcgcggcgc
      121 agcgcaagcc gccctgcgcc atccccttat ggcgttttcg agcggatgta tcgcttcagc
      181 ttcgcttccc tgcggctgcc gcgcgggcgc cacgctcgcg cgaaagcatc gcttgacacc
      241 tcccacgggg ctgaacatga gtaatctcga cgcaagaatc gccgaaacgg agaccgcggc
      301 agctcaattc gagcctctgc ccgaagaaga acgcgccgag ccccggatag cagcgccgcg
      361 gcgcattctg tggcgcgatc acctgcgcat cgggctcggc tccaggtggc tgggaatcgc
      421 cggctatcgc aggggtttgc gtcccgcgct cgattacaag gaagtgatta cgttcgagca
      481 gggcgaggtg gaaagcccgc cgtggcaggc ggcagtggac gcccttcgcg tggctctcgc
      541 cgggacggac ctgagacggc cggcggtgac cgttgtactg tccaaccatt tcgcccgcta
      601 cgcggtcctg ccctggaacg ccgcccttcg aagcgaggtc gagtggctcg cgcttgcgcg
      661 ccatcgcttc agcagtgtgc acggcaccgc ggcggagcag tggcagatcc gaatcgcccc
      721 tgccggccgc gaacgcccgc gcgtggcctg tgcaatcgat gctgtgctgc tcgaagctct
      781 gaagaacgcc gtcaatgggc acggctcgct catctcggcg cagccgtacc tcatggccgc
      841 atacaaccgg ttgcaaccgg tgatcgggca gagctcctgc tggctggtga tcgaagagcc
      901 gggccgcctc accctggcgc tgatccacga tgggacgtgg cgcgccatcc gcagccggcg
      961 caaggacgaa gcgtggcgca tgacgttgcc ccgtctcctc gaccgcgaaa gcgcgctgat
     1021 cgggctggac gttccgtgca cggaagtcgc cgtctgcacg catgttcctt ttgacgagga
     1081 gatgcacggc gcataccggc ttcgcgatct cacctttcct cccggagcgg cggcagcgga
     1141 ccgggaactc gccatggcac tggagtgagc gcaatgtcca aactcgatct cgatttcatg
     1201 aggatgcgcc cggcctcgcc gcggctgcgc tgggcgctct tcgccatcgc ccttgctttt
     1261 gcggcggacc tcggcatcag cttctacagc gtccgtcatc ggatcgcgca aaacgaggaa
     1321 aacctggccg agctgaagcg cggtgcggac ggtgcggcga atgcggcgct cgccatgcgc
     1381 gcaccctcac cggaggagat tcgcgttgcg cgcgacacgg tgcagcggct tgcaatgccg
     1441 tgggacagcc tgtttcgcgc attggaatcc acggcaagcg acaaggtcgc cctgctcgcg
     1501 atcgatcccg aagcgaagtc cgggacggtg gtcatatcgg gcgaggcgac cgactacggg
     1561 gccgcgctcg actacgtcgc ccggcttggc cggtcggggg cgcttgaccg cccgcatctc
     1621 gtgcggcacg aaattgcgcg ggacgaagcg cagaacccgg tgaagttttc gatttccgcg
     1681 aactggagcg aagccaaatg agcgccgccg gctttctgag acgcgtgtgt cacgagctcg
     1741 ggacaatggg ccttgcctcc atcgcgatca tcgcgggcgc gatcctcttt ctcttcacca
     1801 tgctcaagcc gctcgaggcg cgaaatgtgg agctgcagca cgagatcgcg ctgaacgcac
     1861 gtcagaatgc aagcaccgat gccaatcttg tgcggacctc cacgccggcc gcgaagatgg
     1921 ccgccttcta tcattttctg tccaccgacc gcccgatgac cgagtggctg ggacgcctct
     1981 atgcggccgg ccaggccgcg ggggtggagc tgcagtcggc cgactaccgg atgcagaaga
     2041 ccggcacgcg catcgaacgc taccagatca ggattccgct ccgcggcaat tacgctcaga
     2101 tacgcggctt cctcgacaac gccttggtgg acattccggt gctctcgctg gacgaggtga
     2161 agttcaagag ggagcgcgcg agcgatgcgc agatcgaggc cgaactgctg ctttccctgc
     2221 atctcgtcaa cccttgaacg gacgttgcgc acatgaccat gatcgccttc c
//
LOCUS       DASURG010000571         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_402371, whole genome shotgun sequence.
ACCESSION   DASURG010000571 DASURG010000000
VERSION     DASURG010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402371"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /gene="acsA"
                     /locus_tag="VFY80_10365"
     CDS             <1..1688
                     /gene="acsA"
                     /locus_tag="VFY80_10365"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6008358.1"
                     /translation="ACTSFTWQDARGELSGLPGGGLNIAHEAVDRHAATRGDHHALRW
                     IGKGGTRRDFTYAALSALTSRFANVLNGLGCRSGSRVFVLAGRIPELYIAVLGTLKHR
                     SVACTLFSAFGPEPIHTRLTLGAAEILVTTEALYRRKIAEMRSSLPHLRHVLLAGEAG
                     EKTAVADTLDLATLIQGASADYAIPPTSADDPALLHFTSGTTGKPKGALHVHGAVVAH
                     HVTGRLALDLHPQDIFWCTADPGWVTGTSYGIISPLTNGVTSIVDEADFDAERWYSIL
                     GAERVNVWYTAPTAVRMMMKGGAEAARRHEFPDLRFIASVGEPLNPRAVTWGIDAFGL
                     PIHDNWWQTETGGIMIANYAAMDIKPGSMGRPLPGVEAAIVKRVGETGVAVVTEAGVE
                     GELALRAGWPSMFRAYIGEEARYRKCFADGWYLTGDLSKRDRDGYFWFVGRADDVIKS
                     SGHLIGPFEVESALMEHPAVAEAGVIGKPDPMQFETVKAFVSLKAGYEADEPLRRELL
                     GFARKRLGAAVAPKEIDFVATLPRTRSGKIMRRLLKARELGLPEGDTSTLEGA"
     gene            1685..>2502
                     /gene="pdhA"
                     /locus_tag="VFY80_10370"
     CDS             1685..>2502
                     /gene="pdhA"
                     /locus_tag="VFY80_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX6008359.1"
                     /translation="MTIDRARALKLLREMLRIRRFEEKCAELYSAGKIRGFLHLYIGE
                     EAIAAGTMQALAPEDAVLATYREHGHALARGIAPERVMAEMYGKYEGCSRGRGGSMHI
                     FDANTRFYGGSAIVGGGLPLAVGFALADRMMHRPRVTACFFGEGAVAEGEFHESMNLA
                     ALWQVPALFLCENNLYAMGTALERSEAETDLCAKARSYKIAARSVDGMDVLAVMHATT
                     EAVASIHAQDGPQFIELRTYRFRAHSMFDAELYRSKAEVEDWKRRCPILNFTNRL"
BASE COUNT          441 a          772 c          847 g          442 t
ORIGIN      
        1 aggcctgcac ttccttcacg tggcaggatg caaggggcga actgtcgggc ctgccggggg
       61 gcggcctgaa tatcgctcac gaagcggtcg atcgccacgc ggcgacgcgc ggcgatcatc
      121 atgcgctgcg ctggatcggc aagggcggca cgcgccgcga cttcacctac gctgcactga
      181 gcgcgcttac gagccggttc gcaaacgtgc tgaacgggct tggttgccgc agcggatcgc
      241 gggttttcgt gctcgccggc cgaattcccg agctgtacat cgccgtgctc ggcacgctca
      301 agcatcgcag cgtcgcgtgc acattgtttt ccgcattcgg gccggagccg atccataccc
      361 ggcttacgct cggcgcagcg gaaatcctcg tcaccaccga ggcgctgtac cgccgcaaaa
      421 tcgccgaaat gcgcagcagt ctgccgcatc tcaggcacgt tctgctcgca ggtgaagcgg
      481 gggagaagac cgctgttgcc gatacgctgg atctcgcgac gttgatccaa ggcgcgagcg
      541 ccgactacgc cattcccccg accagtgccg atgatcctgc gcttctgcac ttcacgagcg
      601 gtacgactgg caagcccaaa ggggcgctgc acgtgcacgg cgcggtagtc gcccatcacg
      661 tcacggggcg gctcgcgctt gacctgcatc cccaagatat cttttggtgc accgcagatc
      721 ccggctgggt gaccggcaca tcgtacggca tcatctcccc gctcaccaac ggcgtgacga
      781 gcatcgtgga cgaggcggac ttcgatgccg agcgctggta ctcgatcctt ggagccgagc
      841 gcgtgaacgt gtggtacacc gctccaaccg cggtgcgcat gatgatgaag ggcggcgcgg
      901 aggcagcgcg ccgacacgaa tttcccgatc tgcgcttcat cgcgagcgtg ggcgagccgc
      961 tcaacccgcg agccgttacg tggggaatcg acgcgttcgg gctgcccata cacgacaact
     1021 ggtggcagac ggagacgggc ggcatcatga tcgcgaacta cgccgcgatg gacatcaagc
     1081 ccggctcgat gggccgtccg ctccccggcg tggaagccgc gatcgtgaag cgtgtgggcg
     1141 agaccggtgt cgcggtcgtg accgaggcag gcgtcgaggg tgagcttgcg ctgcgtgcgg
     1201 gttggccttc gatgtttcgc gcctatatcg gcgaggaagc gcgctaccgc aagtgctttg
     1261 cggacggctg gtacctgacc ggcgatctct cgaaaaggga ccgcgatggg tatttctggt
     1321 tcgtgggccg cgccgatgac gtcatcaagt cttcggggca tctcatcgga ccgttcgaag
     1381 tggaaagcgc gttgatggag catccggcgg ttgcggaagc gggcgtcatc ggcaagcctg
     1441 acccgatgca gttcgagacg gtgaaggcgt tcgtgtcgct gaaggcagga tacgaagcag
     1501 acgagccgct gcgccgggaa ctgctcggct ttgcgcgcaa gcgcctcggc gcggcggtcg
     1561 cgccgaagga gatcgatttt gtcgcgacgc tgccgcgcac gcgcagcggc aagatcatgc
     1621 ggcgattgct caaggcccgc gagctggggc ttccggaagg cgatacctcc accctggagg
     1681 gtgcatgacg atcgatcgcg cccgagcgct gaagctgctc cgcgagatgc tccgcatccg
     1741 gcgtttcgag gagaagtgcg cggagctcta cagcgccggc aagatccgcg gttttctcca
     1801 tctctacatc ggcgaggaag cgatcgcggc cgggacgatg caggcgctcg cacccgaaga
     1861 cgcggtgctc gccacctatc gtgagcacgg tcacgcgctc gcgcgtggca ttgcgccgga
     1921 acgcgtcatg gcggaaatgt atggcaagta cgaaggctgc tcgcgcggac gcggcggctc
     1981 gatgcatatc tttgacgcga atacgcgctt ttacggcggc agtgccatcg tcggaggcgg
     2041 cctgccgctc gcggtgggct tcgcgctcgc cgacagaatg atgcatcgcc cgcgcgtgac
     2101 cgcatgcttt ttcggcgaag gtgccgtggc ggaaggcgag tttcacgagt cgatgaacct
     2161 cgcggcgctg tggcaggtgc cggcgctttt tctgtgcgag aacaatctct acgccatggg
     2221 cacggcgctc gagcgctcgg aagcggaaac ggacctctgt gccaaggcgc gaagctacaa
     2281 aattgccgcg cgatcggtgg atggcatgga cgtcctcgcc gtcatgcatg ccaccacgga
     2341 agcagtcgcg tcgattcatg cccaagacgg gcctcagttt atcgagttgc gcacctatcg
     2401 cttccgcgcg cattcgatgt tcgatgccga gctgtatcgc tcgaaagcgg aggtcgagga
     2461 ctggaagaga cgctgcccga tactgaattt cacgaatcgg ct
//
LOCUS       DASURG010000572         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_810596, whole genome shotgun sequence.
ACCESSION   DASURG010000572 DASURG010000000
VERSION     DASURG010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810596"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFY80_10375"
     CDS             complement(<1..1036)
                     /locus_tag="VFY80_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6008360.1"
                     /translation="MNDSASSRPTAAPDDPAWYKDAVIYQLHVKAFFDANDDGIGDFR
                     GLASKLDYVRDLGVNTIWLLPFYPSPLKDDGYDVADYHNIHPQYGTRPDFRHMMREAD
                     RRGLRVITELVVNHTSDQHPWFQAARRAPPGSVKRDYYVWSDDPNKYAGTRIIFTDTE
                     ESNWAWDEVAQAYYWHRFFSHQPDLNFDNPRVLKAVFRTMRFWLDMGVDGFRLDAIPY
                     LCEREGTNNENLPETHAVIKQIRRMLDDHYSNRLLLAEANQWPEDVREYFGDGDECHM
                     AYHFPLMPRIYMAIAQEDRHPVVDIMQQTPDIPDSCQWAIFLRNHDELTLEMVTSKER
                     DYMYQMYAADA"
     gene            complement(1038..>2759)
                     /locus_tag="VFY80_10380"
     CDS             complement(1038..>2759)
                     /locus_tag="VFY80_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX6008361.1"
                     /translation="TVTAWVDSFLSWRQDFAKREEETDIELALRSAGRLVEDAAARAK
                     GNEARELTELARELTGGQGFAARRTLALGERLIDLMSRHPDRSLASVHAVEFEIVVDR
                     PLARSSAWYEMFPRSCAASPGCHGTFADCEKRLDYVVEMGFDILYLPPVHPIGYVKRK
                     GRNNTLEARPDDPGSPWAIGSKEGGHKAVHPALGTLEDFRRLVAAARARGLEIALDIA
                     FQCAPDHPYVSEHPEWFQKRPDGSIQYAENPPKKYQDIYPFHFESEAWHALWVELKSV
                     FEFWIEQGVTIFRVDNPHTKPFAFWAWAIAELKREHPEAIFLSEAFTRPRIMHRLAKL
                     GFTQSYTYFPWRNTKAELTEYFSELAHSPAREYFRPNHWPNTPDILTAVLQNGGRPVF
                     MARVVLAATLGANYGIYGPAFEHCENVPREPGSEEYLNSEKYEVRHWNLARPDTLAPL
                     IGRLNRARHENPALQQDWNLIFVPADNPELICYAKFNDARSNVIVTAVSLDPYHKRSA
                     FIEMPLAELGIDADHPYQVEDLLSGESYLWQGARNYVELDPRICPAHVFRVRQQTRSE
                     KDFEYFA"
BASE COUNT          485 a          845 c          890 g          539 t
ORIGIN      
        1 gggcatccgc cgcatacatc tggtacatgt agtcgcgctc cttgctcgtt accatctcga
       61 gcgtgagctc gtcgtggttg cggaggaaga tcgcccactg gcacgagtcc gggatatcgg
      121 gcgtctgctg catgatgtcg acgacaggat ggcggtcctc ctgtgcgatc gccatgtaga
      181 tccgcggcat gagcggaaag tggtacgcca tgtggcactc atcaccatcg ccgaaatatt
      241 cacgcacgtc ttccggccac tgattcgcct ctgcgagcag caggcgattg ctatagtggt
      301 catccagcat gcgccggatc tgcttgatga cggcatgcgt ctccggcaga ttctcgttgt
      361 tcgtcccttc gcgctcgcag aggtagggaa tcgcgtcgag gcggaacccg tccacgccca
      421 tgtcgagcca gaagcgcatg gtgcggaaca ccgccttcag cacgcgcgga ttgtcgaagt
      481 tgagatcggg ctggtggctg aagaaacggt gccagtaata ggcctgcgcg acctcgtccc
      541 acgcccaatt ggactcttcc gtgtcggtga agatgatgcg cgtaccggcg tacttgttcg
      601 gatcgtcgct ccacacgtag tagtcgcgct tcaccgaccc gggcggagcc cgccgcgccg
      661 cctggaacca tggatgctgg tcggacgtgt gattcaccac gagctccgtg atcacacgca
      721 gccctcggcg gtccgcctca cgcatcatgt ggcggaaatc ggggcgggtg ccgtactgcg
      781 gatggatgtt gtggtaatcg gcgacgtcgt agccgtcgtc cttgagtggc gagggataga
      841 acggcagcag ccagatcgtg ttgacgccga gatcgcgcac gtaatcaagc ttggaagcga
      901 gcccgcggaa atcccctatg ccgtcgtcgt tggcgtcgaa aaacgccttg acgtgaagct
      961 gataaatgac agcgtccttg taccaggccg ggtcgtccgg cgccgcggtc gggcggctcg
     1021 aggcggaatc gttcacgtca cgcgaagtat tcgaagtctt tttcggagcg cgtctgctgc
     1081 cgcacgcgga agacgtgcgc gggacagatc cgcggatcga gctcgacgta gttgcgcgcc
     1141 ccctgccaga ggtagctctc cccgctcagg agatcctcga cctggtaggg atggtcggca
     1201 tcgatgccga gctcggcgag cggcatctcg atgaacgccg agcgcttgtg gtagggatcg
     1261 aggcttaccg ccgtcacgat gacattggat cgtgcatcat tgaatttcgc atagcatatc
     1321 agctcgggat tgtcggccgg cacgaagatg agattccaat cctgctgcag ggcgggattc
     1381 tcgtggcgcg cccggttgag ccgcccgatg agcggcgcaa gagtgtcggg acgcgcgaga
     1441 ttccagtgcc gcacctcgta tttttccgag ttgaggtatt cctcgcttcc cggttcgcgc
     1501 gggacgtttt cgcagtgctc gaacgcgggg ccgtagatgc cgtagttcgc gccgagcgtc
     1561 gcggcgagca cgacgcgcgc catgaacacc ggccgcccgc cgttttgcag gacggctgtc
     1621 aggatgtccg gcgtattggg ccagtggttg ggccggaagt actcgcgggc agggctgtgc
     1681 gcgagttcgc tgaagtactc cgtgagctcc gctttggtat tgcgccaggg aaagtaggtg
     1741 taggactggg tgaagcccaa cttcgcgagc cggtgcatga tcctgggtct cgtaaaagct
     1801 tcggagagaa agatcgcctc cggatgctcg cgcttgagct ccgcgatcgc ccaggcccag
     1861 aacgcaaacg gcttggtgtg cggattatcc acgcgaaaga tggtgacacc ctgctcgatc
     1921 cagaattcga agacgctctt caattccacc cacagcgcgt gccacgcttc gctctcgaaa
     1981 tggaagggat agatgtcctg gtacttcttc ggcggattct cggcgtattg gatcgagccg
     2041 tccggccgct tctgaaacca ctccggatgc tcgctcacgt agggatgatc gggcgcgcac
     2101 tggaacgcga tgtcgagcgc gatctccagt ccccgtgcgc gcgcggcggc gaccagacgg
     2161 cgaaaatcct cgagcgtccc gagcgccgga tggaccgcct tgtgcccgcc ttctttcgag
     2221 ccgatcgccc acgggctgcc cgggtcgtcc gggcgtgcct ccaaagtgtt gttgcggcct
     2281 ttgcgtttga cgtagccgat cgggtgtacc ggcggcagat agaggatgtc gaagcccatc
     2341 tccacaacat agtcgagccg cttctcgcag tcggcgaagg tgccgtggca tcccggcgaa
     2401 gcggcgcacg agcgcgggaa catctcgtac cacgcgctcg agcgcgcaag cggccggtct
     2461 accactatct cgaactccac tgcatgaacg cttgcaagcg agcggtccgg atggcgcgac
     2521 atgagatcaa tcaagcgttc gcccagcgcg agtgtccgcc gcgccgcgaa tccctgaccg
     2581 ccggtaagct cgcgcgcgag ttccgtcaat tcccgcgcct cgttgccctt cgcgcgcgcg
     2641 gccgcgtcct cgaccagccg cccagcgctg cgcaacgcga gttcgatatc cgtttcttcc
     2701 tcgcgcttgg cgaagtcctg gcgccaggat aggaaactgt ccacccaggc cgtgacggt
//
LOCUS       DASURG010000573         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1322490, whole genome shotgun sequence.
ACCESSION   DASURG010000573 DASURG010000000
VERSION     DASURG010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322490"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..819)
                     /locus_tag="VFY80_10385"
     CDS             complement(304..819)
                     /locus_tag="VFY80_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008362.1"
                     /translation="MIYGDMETSGNLPNAEYRRFEGMREYEELIDRMIPQTQRTIRIF
                     DRSLSRSYNTPERFELLRQFLLASRVNRLMIALHDAARIERECPRILALMQQFSHAVT
                     IRETLRPAKQVYDPFVIFDASHYVHRFHYEHLRAAQGTNDVLGAQQLIDRFSEIWEAC
                     GPAHSAHVSGL"
     gene            complement(816..1934)
                     /locus_tag="VFY80_10390"
     CDS             complement(816..1934)
                     /locus_tag="VFY80_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6008363.1"
                     /translation="MQRKMLGGMDPAAFLRRHWQKRLLFIARALPQYAHFVTREALFA
                     LAACEAVQSRIVIRGGRRWTVAHGPFSRRTFARLPHRGWTLLVQGVNEVLPRAHELLR
                     AFAFIPYARLDDVMVSYAPAGGGVGPHFDSYDVFLLQIAGTRRWRVSQQRDLELVEGA
                     PLKLLKRFRAEREWKLAPGDMLYLPPQVAHDGIAVDECITASIGFRAPAAGEIASRFL
                     EHLQEEIALDGAYRDPDLKPAKRPAQIGSDMLRKVSRTVDRIDWSAKDVLVFLGRYLT
                     EPKPHVVFARPQRPLPAAAFGREVKRRGIALDLKTQMLFRGRTIFINGERHELGAGAA
                     RLLSRLADRRHLPPRLALDAEAAGCLYQWYRDGYIALA"
     gene            2056..>2458
                     /locus_tag="VFY80_10395"
     CDS             2056..>2458
                     /locus_tag="VFY80_10395"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6008364.1"
                     /translation="MNKTIMLQPGAPAPTFDLPDADMNMVSLESFRNHHNVVLYFYPK
                     DGTPGCTMEAIDFSDLDDEFKSLKTVVLGVSMDDCLSHGSFRDKHGLCVQLLADVEGE
                     VCRKYGVLQEKENDGKRRLGILRSTFIIDRKG"
BASE COUNT          474 a          757 c          795 g          432 t
ORIGIN      
        1 ccggggccgg cgttggagca ggggccggcg tcggggccgc ttcaggtttc ggggcgggcg
       61 ccgggggcgg tgccggctcc gatttcttcg gcggcggggg tggcgcgggc ttgtcgccgc
      121 atccactcaa acccagtcca cccgccagca taaatgccat gatcattgcg cgcttcatgt
      181 tcttttcctc aaagattaaa gagttagacc gactggacct gcggtcggat ggccgcagaa
      241 agttgcaccg tacctatgac atcccaccac acagaaaatt cgatcaattg tgggcgaata
      301 agcttacagc ccgcttacat gcgcggaatg ggcagggccg cacgcttccc agatctcgct
      361 gaagcggtcg atcaactgct gcgcgccgag cacgtcgttg gtgccctgcg ccgcgcgtag
      421 atgctcgtaa tggaagcgat gcacatagtg ggaggcgtcg aaaatgacga acgggtcgta
      481 cacctgcttt gcgggacgaa gcgtctcgcg aatggtgacg gcatggctga actgctgcat
      541 gagtgcgagt atccgcgggc actcgcgctc gatgcgcgca gcgtcatgga gcgcgatcat
      601 gaggcgattc acccgggatg cgagcaggaa ttgacggagt agctcgaagc gctccggcgt
      661 gttgtagctt cggctgagcg aacgatcgaa gatccggatc gtgcgctggg tctgcgggat
      721 catgcggtcg atcaactctt cgtattcccg catgccctcg aagcgacgat attccgcgtt
      781 cggaagattg cccgacgttt ccatgtcacc gtagatcatg ccagagcaat atatccgtca
      841 cgataccact ggtaaaggca cccggccgct tcggcgtcga gcgcaagccg cggcggaagg
      901 tgccgccggt ctgcaagccg cgagagcagg cgcgcggcgc ccgcgcccag ttcgtgccgc
      961 tcgccgttga tgaagatcgt gcgcccgcga aagagcatct gcgtcttgag gtcgagcgcg
     1021 atgccgcggc gcttcacttc gcgtccgaac gccgcagccg ggagcggccg ttgcgggcgc
     1081 gcgaatacga cgtgcggctt gggttcggtg agataccgtc cgaggaagac gagaacatcc
     1141 ttcgcgctcc agtcgatccg atccaccgtg cgggatactt tgcgcagcat gtcgctcccg
     1201 atctgcgccg gccgctttgc gggcttcaag tccgggtcgc gataggcgcc gtcgagcgcg
     1261 atttcttcct gcagatgctc gagaaagcgc gacgcgatct cgcccgccgc aggcgcgcga
     1321 aagccgatcg atgccgtgat gcactcatcg actgcgatgc cgtcgtgggc gacctgcggc
     1381 ggcaggtaga gcatgtcgcc cggagcgagc ttccactcgc gttcggcgcg gaaccgcttc
     1441 agaagcttga gcggcgcacc ttcgacgagc tcgagatcac gctgctggct tacccgccag
     1501 cgccgcgtgc ccgcgatctg cagcagaaac acgtcgtagc tgtcgaagtg ggggcccacc
     1561 ccacccccgg cgggcgcgta gctcaccatg acgtcgtcga gccgcgcgta cggaatgaaa
     1621 gcgaaagccc gaagcaattc gtgcgcccgc ggcagcactt cattcacgcc ctgcacgagc
     1681 agcgtccagc cgcggtgcgg caatcttgcg aaggtgcgcc gcgaaaacgg cccgtgcgcg
     1741 acggtccagc gccggccgcc acggatcacg atacgcgatt gaacggcctc gcacgctgcg
     1801 agcgcaaaga gcgcctcgcg cgtcacgaag tgcgcgtact gcggaagcgc ccgggcgatg
     1861 aacagcaggc gcttctgcca gtgacggcgc agaaacgccg ccgggtccat ccccccgagc
     1921 atcttgcgtt gcatcgtgcg cggattataa gaggggcctg acatggtgaa ccacgtgcgc
     1981 gtttcgcgca tccgtacgga ggcttgaccg gctgacgccg cgctttacaa aattcggata
     2041 gaatcgattc gaggagtgaa caaaaccatc atgctgcaac ccggtgcacc ggcgccgacc
     2101 ttcgatctgc ccgacgccga catgaatatg gtgagccttg agagcttcag gaatcaccat
     2161 aatgtcgtgc tctattttta tcccaaggac ggcacacccg gctgcaccat ggaggccatc
     2221 gacttcagcg atctcgatga tgaattcaag agcctgaaga ccgtggtgct cggcgttagc
     2281 atggacgact gcctgagcca tggatcgttt cgcgacaaac acggtctgtg cgtacagctg
     2341 ctggcggacg tggaaggcga ggtctgccgc aagtacggtg tgctgcagga aaaggaaaac
     2401 gacggcaagc ggcggctcgg cattctgcgc tcgaccttca tcatcgaccg caagggca
//
LOCUS       DASURG010000574         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_709406, whole genome shotgun sequence.
ACCESSION   DASURG010000574 DASURG010000000
VERSION     DASURG010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709406"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VFY80_10400"
     CDS             <1..1030
                     /locus_tag="VFY80_10400"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX6008365.1"
                     /translation="HLLTYIEAILRVYNRYGRRDNLWKARIKILVKDRGAEAFRDEVE
                     QEWGYVKDGPSTLTVEEVDRVAQRFTEPAYETLPARDSGFERRRREDRAFARWVERNV
                     AEHRVPGYAAVTLTLKSTGTPPGDVTSEQMEGVADLADRYSFGELRVTHEQNLLLPYV
                     RRRDLHAVWQRARALKLATPNLGLLTNIISCPGGDFCSLANAKSIPVAEAIQRRFEDL
                     DYVYDIGELDLNISGCMNSCGHHHAGHIGILGVDKQGAEFYQISVGGNQGNDASIGKV
                     IGPSVAQEDVPDVIECLIRAYLSLRDSDHERFIDTVRRVGVEPFKEAVYGSVDQEPRA
                     RSRQLAAA"
     gene            975..1505
                     /locus_tag="VFY80_10405"
     CDS             975..1505
                     /locus_tag="VFY80_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX6008366.1"
                     /translation="MAALIRNRELVQDSWQRLEVAADGGLPAVPQEGDVLVPLALWQR
                     ERPMLLERRGRTGVWLKSSEGPESLAPDIGRLPVIAIDFPKVTDGRGYSTARLLRGRY
                     GYKGELRAIGDVQRDQLLFLSRCGFDAFSLKDGQNAEAALSAFREFSEAYQAGVDRPW
                     PLFRRRGSAPAEVEWS"
     gene            1510..>2294
                     /gene="cobA"
                     /locus_tag="VFY80_10410"
     CDS             1510..>2294
                     /gene="cobA"
                     /locus_tag="VFY80_10410"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX6008367.1"
                     /translation="MSGVAGKVYLVGAGPGAPDLITVRGARVLAEADIVFHDALVHPD
                     TITLALRAKKVAVGKRCGGLATAQRFINKRLVDAARRYRTIVRLKGGDPMLFGRAQEE
                     ISALSRAGIEYEVIPGVTAALGAAAEAGISLTQRGIARSVAVLTPRVGEGQSQSDWVK
                     CAIAADTAVLYMAAGQASEIADALIAHGRSPDTPLVVVENATLPGARRFFIRLSQLPS
                     IATFGIEGPALIMLGAVFAQGVGAFDESLQAFPIENETRYNSS"
BASE COUNT          412 a          725 c          754 g          403 t
ORIGIN      
        1 gcacctgctc acctatatcg aagccatatt gcgcgtctac aaccgctacg gccgccgcga
       61 caacctgtgg aaggcgcgca tcaagatcct ggtcaaggat cgcggcgctg aggctttccg
      121 ggacgaggtc gagcaggaat ggggctatgt caaagatggc ccttcgacgc tgacagtgga
      181 ggaggtggac cgtgtggcgc agcgattcac cgagcctgcc tacgagacgc ttcccgcgcg
      241 cgacagcggg ttcgagcgca ggcgccgcga agaccgggcc tttgcgcgct gggtcgagcg
      301 caacgtggca gaacatcgcg ttcccggcta cgccgccgtg acgctcacgc tgaagagtac
      361 cggcacgccg cccggagacg tcacatccga gcagatggaa ggcgtcgccg atctcgcaga
      421 ccgctacagt tttggcgagc tgcgggtgac gcacgaacag aacctgctgc tgccgtacgt
      481 gcgccgacgc gatctccacg cggtgtggca gcgtgcgcgc gcgctcaagc tcgccacgcc
      541 caacctggga cttctgacca acatcatctc gtgccccggc ggggatttct gctcgcttgc
      601 caacgcgaaa tcgattccgg tggccgaagc gatacaaagg cgcttcgagg atctcgatta
      661 cgtttacgac atcggcgagc tcgacctcaa catttcgggc tgcatgaatt catgtggcca
      721 ccatcacgcc ggtcatatcg gcatcctggg cgtggacaag cagggagcgg agttctatca
      781 gatttcggtc ggcggcaatc agggcaacga cgcgtcgatc ggcaaggtca tcggtccctc
      841 cgtggcgcag gaagacgtcc ccgacgtgat cgaatgcctg attcgagcct atctcagcct
      901 ccgcgacagc gaccacgagc gcttcatcga taccgtgcgt cgcgtcggcg tggagccctt
      961 caaggaagcg gtctatggca gcgttgatca ggaaccgaga gctcgttcaa gacagctggc
     1021 agcggcttga agtcgcggcc gacggcgggc tgccggcggt cccgcaagag ggcgatgtcc
     1081 tcgttccgct tgcgttgtgg cagcgcgagc ggccaatgtt gctcgagcga cgcggccgca
     1141 ccggcgtgtg gctgaagagc agtgaaggcc cggaatcgct cgcgccggat atcgggcggc
     1201 ttccggtcat cgcgatcgac tttccgaagg tcaccgacgg acgcggctat tcgaccgcgc
     1261 gcctgctgcg cgggcgctac ggctacaaag gggagctgcg cgcgatcggc gacgtgcagc
     1321 gcgaccagtt gctcttcctc tcccgttgcg ggttcgacgc cttctcgctc aaagacgggc
     1381 agaacgccga ggcggcgctc tctgcattcc gcgaattcag cgaagcgtat caggctggag
     1441 tcgatcgccc gtggccgctt ttccgccgac ggggctcggc tcccgccgag gtcgaatggt
     1501 cttgaacgca tgagcggcgt agccggaaaa gtctatctcg tcggcgcggg accgggcgcg
     1561 ccggatctca tcaccgtgcg cggcgcgcgc gtgcttgctg aagccgacat cgtgtttcac
     1621 gatgcgctgg tgcatcccga tacgattacg ctggcgctgc gcgcaaaaaa agttgcggtg
     1681 ggcaagcgtt gcggcgggct tgcgacggca cagcgtttca tcaacaagcg cctggtcgat
     1741 gccgcgcgcc gctaccggac gatcgtgcgg ctgaaaggcg gcgatcccat gctcttcggc
     1801 cgtgcgcagg aggaaatctc ggcattgagt cgagccggta tcgaatacga ggtgatcccc
     1861 ggcgtcaccg ctgcactcgg ggccgcggcg gaagcgggca tttcgctcac gcagcgcggg
     1921 atcgcccgca gcgttgcggt gctcacgccg cgcgtggggg aaggacaatc gcaaagcgac
     1981 tgggtgaagt gcgcgatcgc agccgacacc gctgtactct acatggccgc gggccaggca
     2041 tcggagatcg cggacgcgct cattgcgcat ggccgttcgc ccgatacacc gctcgtcgtg
     2101 gtggaaaacg cgacgcttcc cggtgcccgg cggtttttca tccgactttc gcaactgccg
     2161 tcgatcgcaa ccttcggcat cgagggaccg gcgctgatca tgctcggcgc cgtcttcgca
     2221 caaggtgtcg gcgctttcga cgaatccctt caggctttcc caatcgaaaa cgaaacacgc
     2281 tacaattcaa gctt
//
LOCUS       DASURG010000575         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_302699, whole genome shotgun sequence.
ACCESSION   DASURG010000575 DASURG010000000
VERSION     DASURG010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302699"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VFY80_10415"
     CDS             <1..1635
                     /locus_tag="VFY80_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX6008368.1"
                     /translation="VARVLGVMGLVSIGFLTFMLFTSNPFDRQIPAAPDGRDLNPLLQ
                     DPGMVVHPPMLYMGYVGFSVSFAFAIAALLHGRLDAAWARWSRPWTTAAWCFLTLGIM
                     LGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGAFKAWTVLLAIL
                     AFALSLLGTFLVRSGVLTSVHAFATDPKRGIFILGFLTIVIGGSLLLFAWRAPRVGIG
                     GAFAPVSREGSLLLNNVLLVVAAGSVLLGTLYPLALDAFGLGKISVGPPYFEAVFVPL
                     MAPVIFLMGVGPVMRWKSMPIPELAKLLRWAFGVSLVSGAVLPVVFGHWSFLLALGFL
                     LAVWVVASCAVSLWRRLRGERSAAGLWTRLRGSSRSYYGMLLAHFGIAIFVFGVTLVK
                     GYETKTDVRMAPGDTATLGAYTFRFDGTREVRGPNYAAARATIHVTRDGRAVTVLYPE
                     KRLYTVQQQQMTEAAIHTRPARDLYVSLGDPLPGTTDTWLVHIQYKPFIVWLWIGCVV
                     MALGGALAASDRRYRVGARAPDVSPAGGKPRVAAAG"
     gene            1645..2196
                     /locus_tag="VFY80_10420"
     CDS             1645..2196
                     /locus_tag="VFY80_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX6008369.1"
                     /translation="MSEATAPALLRYGLPLGVFLLLVALLAIGLNLNPREVPSPLIDK
                     PAPAFELPQLHAQDRSFSPASMKGQVWILNVWASWCAACLEEHPVFLDFAKRRVVPLV
                     GLNYKDRREEALKWLKRHGDPYTLSAVDLNGRVGIDYGVYGVPETYVIDKQGVIRYKR
                     IGPVTPRILETKILPLVSELNRG"
BASE COUNT          305 a          700 c          729 g          487 t
ORIGIN      
        1 gtcgcgcgtg tgctgggcgt catggggctc gtaagcatcg gctttctcac cttcatgctc
       61 ttcacgtcca atccgttcga ccgtcagatt cctgcggcgc cggacgggcg cgatttgaat
      121 ccgctcttgc aggatcctgg catggtggtt catccgccca tgctttacat gggttatgtg
      181 ggattttctg tgtcgttcgc cttcgcgatc gccgcccttc tgcacggtcg cctggatgcc
      241 gcctgggcgc gctggtcacg cccgtggacg accgcggcgt ggtgctttct caccctcggt
      301 atcatgctcg gcagcgcatg ggcttattac gaattgggct ggggcggctg gtggttctgg
      361 gacccggtcg agaacgcttc cttcatgccg tggctcgtcg gcacggcgct gatccactcg
      421 ctcgcggtta ccgaaaagcg cggcgccttc aaggcatgga cggtgcttct tgccattctg
      481 gcgtttgcgc tgagcctgct cggcactttc ctcgtgcgct cgggggtctt gacttcggtg
      541 cacgccttcg cgacggatcc caagcgcggc atttttattc tgggctttct caccatcgtg
      601 atcggcggct ccttgctgct cttcgcctgg cgtgcgccgc gggttggcat cggcggcgcc
      661 ttcgcgccgg tatcgcggga aggctcgctc ctcctcaaca acgtgctgct cgtggtggcc
      721 gcgggctcgg tgctgctcgg cacgctctat ccgctcgcgc tcgatgcgtt cgggctcgga
      781 aagatttccg tcggcccgcc ctatttcgaa gccgtgttcg tgccgctcat ggcgccggtt
      841 atttttctca tgggagtggg tccggtgatg cgctggaaga gcatgcccat tcccgaactg
      901 gcgaaactcc tccgctgggc gttcggggtg agcctcgtga gcggggccgt gctgccggtc
      961 gtcttcgggc actggtcgtt cctgctggcg ctgggattcc tgctcgccgt ctgggtggtc
     1021 gcgagctgcg cagtgagtct gtggcggcgc ctgcgcggcg aacgcagcgc ggccggtctc
     1081 tggacgcgac tgcgcgggag ttcgcgcagt tactacggga tgctgcttgc gcacttcggt
     1141 atcgcgatat tcgttttcgg cgtcacgctg gtgaagggct acgagacgaa gaccgatgtg
     1201 cgcatggcgc ccggggacac tgcgacgcta ggtgcctaca ccttccgctt cgatggaacg
     1261 cgcgaagtcc gcgggcccaa ttatgctgcg gcgcgcgcta ccattcacgt tacgcgcgac
     1321 ggccgtgcgg tcaccgtgct ctatcccgag aaaaggctct acacggtgca gcagcagcag
     1381 atgaccgagg cggcgatcca cacgcggccc gcccgcgacc tctacgtctc gctcggcgat
     1441 ccgcttcccg gaacgaccga cacatggctc gtgcacatcc agtacaagcc cttcattgtg
     1501 tggctgtgga ttggatgcgt ggtgatggcg ctcggtggcg cgctcgctgc gtcggatcgg
     1561 cgctatcgtg tcggcgcacg ggcgccggac gtgagtcccg caggcgggaa gccgcgcgtc
     1621 gccgccgccg gctgatggcg agagatgtct gaggccaccg ctcccgcgct cctgcgctac
     1681 ggactgccgc tcggcgtttt cctgctgctc gtggcgctgc tcgcgatcgg gctcaatctc
     1741 aacccgcgcg aggtaccttc acccctgatt gacaagcctg cccctgcctt cgagctgccg
     1801 caactgcatg cccaggatcg gagtttttcg cctgcttcca tgaaagggca ggtgtggatc
     1861 ctgaacgtgt gggcatcgtg gtgcgcagcc tgcctcgagg agcatccggt ctttctcgat
     1921 ttcgcgaagc ggcgcgtggt gccgctcgtg gggctcaatt acaaggatcg gcgcgaggag
     1981 gcattgaaat ggctgaagcg ccacggcgat ccgtacacgc tctccgccgt cgatctcaac
     2041 ggccgcgtgg gcatcgatta cggcgtgtat ggcgtaccgg agacgtacgt gatcgacaag
     2101 cagggagtga tacgctacaa gcgcatcggc ccggttacgc ccagaatact cgagaccaaa
     2161 atcctgcctc tcgtgagcga gcttaatcgt gggtaagtgc agcatctttc ctggactccg
     2221 g
//
LOCUS       DASURG010000576         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_608850, whole genome shotgun sequence.
ACCESSION   DASURG010000576 DASURG010000000
VERSION     DASURG010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608850"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFY80_10425"
     CDS             complement(<1..803)
                     /locus_tag="VFY80_10425"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX6008370.1"
                     /translation="MNAETKKVVIAGGGTAGWMAAAALSQQFQGLVEVTLIESDEIGT
                     VGVGESTIPPIRTFHRLLGIDEQEFMRATAATFKLGISFEDWKRPGERYIHSFGMNGK
                     STWVCEFHHFWLHSLTRGIQSELGDYCLELQAARREKFATSAQSDINFAYHLDASVYA
                     KFLRKFSEGHGARRVEGKIRGVRQNAESGFIEALVLESGQVIEGDLFIDCTGFRGVLI
                     EQALHTKFQDWTHDLPCDRAVAVQTESVGPAVPYTRAIAHEAGWRWRIPL"
     gene            complement(805..1827)
                     /locus_tag="VFY80_10430"
     CDS             complement(805..1827)
                     /locus_tag="VFY80_10430"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX6008371.1"
                     /translation="MLQVAGKIRVLEGYRPENLPLHELLARGEPAVLKGLVDEWELVR
                     AGRNSDEDAMHCLRSHYNGRPVRYSYGEPQIAGRPFYSEDFTQLNCIVRQAGLDQILD
                     EIHRHLHDERPPTYYVASLLVDGHLPGFRARNDLDFAAHGLNAPPSIWIGNRTIASCH
                     YDAPNNIACCAIGRRRFTLFPPEQIFNLYPGPLDPSPGGQAVSVVDFANPDFGKYPRF
                     RAAMAAGQMAEVEPGDAVFIPAMWWHHVEGLSAFNALVNYWWSSVPDFIPSPMNALYE
                     LIWSLRDRPEREKLAWKNVIEYYVLGPAGRAGEHLPEPARGVLGPLDETMARQMRAML
                     LNRLNR"
     gene            complement(1831..2580)
                     /locus_tag="VFY80_10435"
     CDS             complement(1831..2580)
                     /locus_tag="VFY80_10435"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX6008372.1"
                     /translation="MARHVMLDNVTHRDLRVITRYGPQFGDDVGTVLTFPTEYADIQR
                     EYPIFFRKDPASGEYHSVALLGFGKNENLFLEQDRWLADYVPGIVVRGPFLIGFQERD
                     VGGELRREPMIHVDLDHPRVSHTEGEPVFLPKGGNSPYLDHIALVLRGIHAGLEMSKA
                     MFAAFAALDLIEPVSLDVKLSAEEGYSVRGLHTVDQRKLAALDGASLERLNRAGFLQG
                     AFLVIASMGNVKKLMAMKQRKRAAQVSATAR"
     gene            complement(2681..>2987)
                     /locus_tag="VFY80_10440"
     CDS             complement(2681..>2987)
                     /locus_tag="VFY80_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX6008373.1"
                     /translation="TANAVLIYEKYGITARLAYNWRDEFLAATNQNGSNTNPFFVEAY
                     DQIDLSVGYQFNDSLSFQVEAVNLTGEDVRWHARSEKQVVRLEDQQPRYAVGVRYKF"
BASE COUNT          542 a          967 c          931 g          547 t
ORIGIN      
        1 agcggaatgc gccagcgcca gcccgcctcg tgcgcgatcg cgcgcgtgta gggcactgcc
       61 ggtccgaccg attccgtttg caccgctacg gcgcggtcgc acggcagatc gtgcgtccag
      121 tcctgaaact tcgtgtgcag cgcctgttcg atcagcacac cgcgaaagcc ggtgcagtcg
      181 atgaagagat ctccttcgat gacctggccc gattcgagca ccagcgcctc gatgaagccg
      241 gattcggcat tctgtcggac gcccctgatc ttgccctcga cccgcctggc accatggccc
      301 tcggagaact tgcgcaggaa ctttgcgtaa accgaggcat cgaggtgata cgcgaagttg
      361 atgtccgact gggcggacgt cgcgaatttc tcgcggcggg ccgcctgcag ctccaggcag
      421 taatcgccca gttccgactg gatgccccgg gtgaggctgt gcagccagaa atggtgaaac
      481 tcacaaaccc aggtcgactt cccgttcatg ccgaaggaat ggatgtagcg ctcgccggga
      541 cgcttccagt cctcgaacga gataccaagc ttgaaggtcg ccgcggtagc tcgcatgaac
      601 tcctgctcgt cgatgccgag cagtcgatgg aacgtccgaa tcggtggaat cgtggattcg
      661 cccaccccga cggtgccgat ctcatcggac tcgatcagcg taacctccac gaggccctgg
      721 aactgctgtg acagcgctgc tgcagccatc catccggctg tcccgccgcc cgcgatcacg
      781 accttctttg tttcggcgtt catgtcagcg attgagcctg ttgagcagca tggcccgcat
      841 ctgccgcgcc atcgtttcgt cgagggggcc aagcacgccg cgcgcaggct ccggcaggtg
      901 ttcgccggcg cgccccgccg gacccagcac gtaatattcg atgacgttct tccaggccag
      961 cttctcccgc tcgggccggt cccgcagcga ccagatcaat tcgtagagcg cgttcatcgg
     1021 cgagggaatg aagtccggca ctgagctcca ccagtagttc acgagcgcat tgaacgcact
     1081 caatccttcc acgtggtgcc accacatggc ggggatgaac acggcgtcgc cgggctccac
     1141 ctcggccatc tgccccgcgg ccatcgctgc gcgaaaacgc ggatacttcc cgaagtcggg
     1201 attcgcgaaa tcgacgacgc tgacggcctg cccgcccggc gaggggtcga gcggcccggg
     1261 atagaggttg aagatctgct ccggcggaaa cagcgtgaag cggcgtctgc caattgcgca
     1321 gcacgcaatg ttgttcggcg catcgtagtg gcaggaggcg atcgtgcgat tgccgatcca
     1381 gatgctcggc ggtgcattca ggccatgcgc cgcgaaatcc agatcgttcc tcgctctgaa
     1441 accgggcagg tgaccatcga ccaacaatga agcgacgtag taggtcggcg ggcgctcatc
     1501 gtgcaggtgg cggtggatct cgtcgagaat ctgatccagg cccgcctggc gcacgatgca
     1561 gttcaactgc gtgaaatctt cgctgtagaa ggggcgaccc gcgatctgcg gctcaccgta
     1621 ggagtagcgg accggcctgc cgttgtagtg cgagcgcagg cagtgcatgg cgtcctcgtc
     1681 ggagttgcgc cccgcgcgca cgagctccca ctcgtcgacg agtcccttca gcacggccgg
     1741 ctcgccgcgg gccaacaatt cgtgcaacgg caggttttcc ggccgatagc cttccagtac
     1801 gcgaattttt ccggcgacct ggagcatggg tcagcgagcc gtcgcggaca cctgcgctgc
     1861 ccgcttgcgc tgcttcatgg ccatcagctt cttcacattg cccatcgatg cgatcacgag
     1921 gaaggcaccc tggagaaatc cggctctgtt cagccgctcg agcgacgccc cgtcgagcgc
     1981 cgccagcttg cgctgatcga cggtatgcag accacggacg ctgtatccct cctcggcgct
     2041 caacttgacg tcgagcgaga cgggttcgat gaggtccagc gccgcgaacg cggcgaacat
     2101 ggccttgctc atctccaggc ctgcatggat gccgcgcaac accagcgcga tgtggtccag
     2161 gtacgggctg ttgccgccct tgggcaggaa taccggctcg ccctcggtgt ggctgacgcg
     2221 cggatgatcc agatccacgt ggatcatcgg ctcccgtcgc agctcaccgc cgacgtcgcg
     2281 ctcctgaaag ccgatgagga agggaccgcg cacgacgatg cccgggacat agtccgcgag
     2341 ccagcgatcc tgctccagaa acaggttctc gttcttcccg aagccgagca gcgcgaccga
     2401 atgatactcg ccgctcgcgg gatcctttct gaagaagatc ggatactcgc gctggatgtc
     2461 ggcgtactcg gttggaaagg tgagtaccgt gcccacgtcg tcgccgaatt gcgggccata
     2521 gcgcgtgatc acccgcagat cccggtgggt gacgttgtcg agcatgacgt gcctggccat
     2581 acggtagtca caagactaaa cggaaaaggc cgctgagagt cagcggccct tccctcgagc
     2641 gcgcatcgag cgtggggggg cgccctatgc acgctcggcg tcagaacttg tagcgaacgc
     2701 cgacagcata gcgcggctgc tgatcctcca gcctgaccac ctgcttctcc gatcgggcgt
     2761 gccagcggac gtcctcaccc gtcaggttga ccgcttccac ctggaacgac aggctgtcgt
     2821 tgaactgata cccgacgctc aggtcgatct ggtcgtacgc ctcgacgaag aaaggattcg
     2881 tgttggatcc gttctgattc gtcgccgcca ggaactcgtc gcgccagttg taggccaggc
     2941 gcgcggtgat gccgtacttc tcgtagatca gcaccgcgtt ggcggtg
//
LOCUS       DASURG010000577         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_404299, whole genome shotgun sequence.
ACCESSION   DASURG010000577 DASURG010000000
VERSION     DASURG010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404299"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /gene="tsaE"
                     /locus_tag="VFY80_10445"
     CDS             <1..315
                     /gene="tsaE"
                     /locus_tag="VFY80_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6008374.1"
                     /translation="GGLGHAGKVRSPTFTLVELYKFSKLYLYHFDFYRFEDPRELDDA
                     GFREYFNAASVCLVEWPEKAAGHLPAADVTVALRNEGDGRRVEMAGHTEAGRRCIAQL
                     GH"
     gene            291..1622
                     /locus_tag="VFY80_10450"
     CDS             291..1622
                     /locus_tag="VFY80_10450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6008375.1"
                     /translation="MHRATRTLNVERLAAPLRQPCFVWYLVCAIVSVLSLIPTVHAEG
                     ITISAARVWPAADYTRLTLESHAAIPHNLFGVKDPERLVLDLEGVEITAALTEIADRI
                     SRDDPYIKAVRIGRYKPGVVRLVLDLKTEVKPQAFILPPVGEYGHRLVLDLYPLVPPD
                     PLLAFLESRRAPESDPGVEPATPSPEPASVEAAPPRRAKRGEPRSKRLIIVAVDAGHG
                     GEDPGALGRSGTHEKDVTLAIARRLKARIDREKNMRAVLIRDGDYYIPLHMRVQKARR
                     VNADVFVSIHADAFIRPHARGSSVFALSERGATSVAANWLAKRENDADLIGGVNLDVP
                     DPYLKRTLLDLSQTATINDSLKLAKAVLSELGEINTLHKPHVEQAGFAVLKAPDIPSI
                     LVETAFISNPQEERKLRDPAYQNRMADAILTGLKRYFAQNPPLAREQLARN"
     gene            1788..2231
                     /locus_tag="VFY80_10455"
     CDS             1788..2231
                     /locus_tag="VFY80_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX6008376.1"
                     /translation="MVGVQDGDTLTVLIDRRQVRVRLVEIDAPELGQPFGTRAKQSLS
                     ELCYGRRAELDIRGRDRYGRTLAQVSCGGKDANAEQVVRGLAWTYVRYVTPDSPLHAL
                     EREARAARRGLWGDPQPIPPWNWRRNGRHSTVDPEASRLTTAPAL"
BASE COUNT          442 a          806 c          749 g          426 t
ORIGIN      
        1 ggtggcctcg ggcatgccgg aaaagtgcgc agtccgacct ttaccctggt tgaactttat
       61 aaattttcta agttatactt gtatcacttt gatttttatc ggttcgagga tccacgagaa
      121 ctcgacgacg caggctttcg cgaatacttc aacgcggcgt cggtatgtct cgtggaatgg
      181 ccggagaaag cggcgggcca tctgccggct gctgacgtaa ccgtcgccct caggaacgag
      241 ggcgatgggc ggcgggtcga gatggcggga catacggagg cgggcaggcg ttgcatcgcg
      301 caactcggac actgaatgtg gaaaggctgg cggccccact gcgccagccg tgcttcgttt
      361 ggtatctggt gtgcgcgatc gtcagtgtct tgtccctcat cccgaccgtc catgccgaag
      421 gcataacgat ctccgcggcg cgggtatggc ccgccgctga ttacacccgg ctcacgctcg
      481 aaagccacgc agcaattccg cacaatctct tcggcgtcaa ggaccccgaa cgccttgtac
      541 tcgatctcga gggcgtcgag atcaccgctg cactgaccga gattgcggac cgcatatcca
      601 gggacgatcc atacatcaaa gcggtgcgca tcggtcgcta caagccgggc gtggtacgcc
      661 ttgtgctcga tttgaagacg gaggtgaagc cgcaggcgtt cattctgccg ccggtcgggg
      721 agtacggcca tcgcctcgtg ctcgatctgt acccgctcgt cccgccggat ccgctgctcg
      781 cctttctcga atcacgccgt gcgcccgaaa gcgatcccgg cgtggaaccg gcgacacctt
      841 ccccggagcc ggcatcagtc gaagccgcgc cgccgcgtcg ggcgaaacgc ggtgagccgc
      901 gctcgaagcg cctcatcatc gtggccgtgg acgcggggca tggcggcgag gatcccggcg
      961 ccctgggacg cagcgggacc cacgagaaag acgtgacgct cgccatcgca cgccgactca
     1021 aggcacgcat cgaccgcgag aaaaacatgc gggcggtgct gatccgcgac ggcgactact
     1081 acattccgct gcacatgcgg gtgcagaagg cgcgccgggt aaacgccgat gtcttcgtct
     1141 cgatccacgc cgatgcgttc atccggccgc acgcgcgcgg gtcgtcggtg ttcgccctgt
     1201 ccgagcgcgg cgcgacatcg gtcgccgcga actggctcgc gaagcgcgaa aacgacgcgg
     1261 acctgatcgg cggggtcaac ctcgatgtgc cggatcccta tctcaagcgc accctgctcg
     1321 acctctcgca gaccgcgacc atcaacgata gcctgaagct cgcgaaagca gtgctttccg
     1381 agctgggaga aatcaacacc ctccacaagc cgcatgtcga gcaggcaggc tttgcggtgc
     1441 ttaaagcgcc ggacattcct tcgatcctcg tggaaacggc gttcatcagc aacccgcagg
     1501 aagaacgcaa gctccgcgat cccgcctacc agaaccgcat ggcagacgcc atcctgaccg
     1561 gcctcaagcg ctatttcgcg cagaatccgc cgctcgcgcg cgagcagctc gcccgaaatt
     1621 gatccgtatg gaacggttct tcgcctgcat tgcacacccg ccacgatgtc gggtatcgca
     1681 cacggggcat tgaattgacg ccccgtccaa ttcctacccg cttacttctc tcgctctttc
     1741 ttccccttct taccgcactt cccgctcagg ccgcgccgca ggcaccggtg gtaggcgtgc
     1801 aggacgggga taccctgacg gtgttgatcg atcgccggca agtgcgcgtg cggctcgtcg
     1861 aaatcgatgc accggaactc gggcagcctt tcgggacgcg cgcgaagcaa tcgctgagcg
     1921 agctgtgcta cggccgccga gcggagctgg acatccgcgg tcgcgaccgc tacggccgca
     1981 cgctcgccca ggtgagctgc ggcggcaaag atgccaacgc cgagcaggtc gtccgcggat
     2041 tggcgtggac gtacgtgcgc tacgttacac cggattcgcc tctacatgcc ctcgagcgcg
     2101 aggcgcgcgc tgcccgccgc gggctgtggg gtgatcctca acccatccca ccgtggaact
     2161 ggcggcgcaa tggccggcac tcgacggtgg accccgaggc gtcgcgactt accaccgcgc
     2221 ccgcgctgta aaccgcgcgc gccttgaatt tcacgcaacg tgggtggatg cgatctatcg
     2281 cagaggtgtc atgaggctca ggctgcctaa ggcggccgcg accgagccgc gcagcatcgc
     2341 aaaccgctga gcgacgctcg acgcgccgcc caccggcgct actccagcga tttgacgatg
     2401 tcttcgacga ctttctttgc gtc
//
LOCUS       DASURG010000578         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_914957, whole genome shotgun sequence.
ACCESSION   DASURG010000578 DASURG010000000
VERSION     DASURG010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914957"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFY80_10460"
     CDS             <1..465
                     /locus_tag="VFY80_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX6008377.1"
                     /translation="GTLHFSEAEVERVREYLLKGGFIWVDDSWGSRAIESWEREIGRV
                     LPPNEYPIVNIAPDHPVFRMMFEVTRLPQIPSISHWRRSGGGTSERGPDSDTPMIRGI
                     SDAHGNLLVLMTHNTDISDAWEREGEDPEFFYSFSPEGYAVGLNIVLYAMTY"
     gene            complement(489..1130)
                     /locus_tag="VFY80_10465"
     CDS             complement(489..1130)
                     /locus_tag="VFY80_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX6008378.1"
                     /translation="MLPATLPLFPLPNVVLFPNVFLPLHIFEPRYRQMVSDALAGERM
                     IGMVLLQPGYEPNYEGTPAVYEIGCAGLITHVERLGDGRFNIVLRGLERFRIVGEEPP
                     NANVLYRRAMVAPMSEVNPPNRSDLLKDARQRLEALLAPLFTGTLAARGLPQQMPDDE
                     LINALAQYLELEPIEKQALLERDGALQRCQSLVELLEMKALMEKGLGKAGQRH"
     gene            complement(1204..>2052)
                     /locus_tag="VFY80_10470"
     CDS             complement(1204..>2052)
                     /locus_tag="VFY80_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6008379.1"
                     /translation="LDFLGYKALRDLLGSIGHSSAGRHDTRDLATGIDASGAPKPYEF
                     GDTMNLDATATILNAVKRRAHDGRSSGTLDITYQDLMVAQGEYQSSCATVLMLDCSHS
                     MILYGEDRFTPAKRVAMALSHLIRTQYPGDTLRAVLFHDSAEEIPLRELGRVKVGPYY
                     TNTREGLRLARRILEKQRKDMRQIVMITDGKPSALTQPDGRIYKNAFGLDPFVIAETF
                     AEVSACARAGIMINTFMLARDYDLVAFVRRVAAMCRGKAYFTTPHTLGEYVLTDYMEK
                     KTRTIH"
BASE COUNT          354 a          645 c          666 g          387 t
ORIGIN      
        1 ggcacgctcc acttctccga ggcggaagtc gagcgcgttc gcgagtatct gctcaagggc
       61 gggttcatct gggtcgacga ctcgtggggc tcgcgcgcca tcgagtcgtg ggagcgcgag
      121 atcgggcgtg tgctgccgcc caacgagtac ccgattgtga acattgctcc tgatcacccg
      181 gtgttccgga tgatgttcga agtcacgcgg ctgccgcaga tcccgtcaat ctcgcattgg
      241 cggcgtagcg gcggtggcac gtcggaacga ggtccggaca gcgacacccc gatgattcgc
      301 ggcatcagcg acgcgcacgg caacctgctc gtgttgatga cgcacaacac cgacatctcg
      361 gatgcctggg agcgcgaagg cgaggatccg gagttcttct acagcttctc gccagagggg
      421 tatgcggtgg ggttgaacat cgtgctctac gcgatgacgt actgaggatc gggaactttc
      481 gccgatcgtc agtgccgttg ccccgctttc ccgagccctt tttccatcag cgccttcatc
      541 tcgagcagct ccaccaatga ctggcatcgc tgcagcgcgc catcgcgctc gagcagtgcc
      601 tgcttctcga tcggctcgag ctcgagatac tgcgccagcg cgttgatgag ttcgtcatcg
      661 ggcatctgtt gcggcagacc gcgcgccgcc agcgtcccgg tgaacagcgg cgcgagcaac
      721 gcctcgagac gctgcctcgc gtctttgagc aggtcggagc gattgggcgg attgacttcg
      781 ctcatcggcg ccaccatcgc ccgccggtag aggacgttcg cattcggcgg ctcctcgccg
      841 acgatgcgga agcgctcgag gccgcggagg acgatgttga agcggccatc gccgagacgt
      901 tcgacgtgcg tgatcaagcc cgcgcagccg atttcgtaca ccgccggcgt gccctcgtag
      961 ttcggctcgt aaccgggctg cagcagcacc atgccgatca tccgctcgcc ggccagcgcg
     1021 tcgctcacca tctgccggta acgcggctcg aagatgtgca gcggcaggaa cacattcgga
     1081 aacagcacga cgttcggcag cgggaagagg ggcagggtcg caggcagcat gggcgttgtc
     1141 attggaggcg attggtcatc gctcgatcgc gtgcaccgcg acgcacgaac caaccactcg
     1201 ctctcaatgt attgtgcgcg tctttttctc catgtagtcc gtgagcacgt actcgccgag
     1261 ggtgtgcggc gtcgtgaagt acgccttgcc gcggcacatc gcggccacac ggcgtacgaa
     1321 cgccaccaga tcgtagtcgc gcgccagcat gaaggtgttg atcatgatcc cggcacgagc
     1381 gcacgcagag acttcggcaa acgtttcggc gatgacgaac ggatcgaggc cgaatgcgtt
     1441 cttgtagata cgcccgtccg gctgcgtcaa tgccgagggt ttgccgtcgg tgatcatcac
     1501 gatctgccgc atgtccttgc gctgcttctc gaggatccgg cgcgccagac gcagcccctc
     1561 tcgcgtgttg gtgtagtacg gccccacctt gacgcgtccg agctcgcgca acgggatctc
     1621 ttccgcggaa tcatgaaaca gcacggcgcg aagcgtgtcg ccggggtact gcgtccggat
     1681 caagtgtgac agggccatcg ccacgcgctt cgccggcgtg aagcgatcct cgccgtagag
     1741 gatcatgctg tgactgcaat cgagcatcag gaccgtcgcg cacgagctct gatactcgcc
     1801 ctgggccacc atcaggtcct gatacgtgat gtcgagggtg cccgacgaac ggccgtcgtg
     1861 agcgcggcgc ttcaccgcgt tgagaatggt ggcggtggcg tccagattca tcgtgtcgcc
     1921 aaactcgtac ggcttgggtg cgccgctcgc gtcgatgccg gttgccaaat cgcgcgtgtc
     1981 gtgccgcccg gcactgctgt ggccgatcga gccgagcaga tcgcgcagtg ccttgtagcc
     2041 gagaaagtcg ag
//
LOCUS       DASURG010000579         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_98071, whole genome shotgun sequence.
ACCESSION   DASURG010000579 DASURG010000000
VERSION     DASURG010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98071"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFY80_10475"
     CDS             <1..1117
                     /locus_tag="VFY80_10475"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6008380.1"
                     /translation="RCQKYIPCEVVKNAPWQAVTLEGDEVDLAKLPIPLHFSVDAAPY
                     ITAGQIVARDPVTGVDTTGFHRLMVKGRNRLGVSLHSRRRMHEFHRRAAERGQNLPAA
                     ITLGIHPLHYMGAMVYAYPPHVRKYEIIGGLFGEPYQLASTGFNDLEVPLGAEIVIEG
                     EILADVHEPEGPFSEFTGYASYRSTENVFVVKRIRMRRDAMYHSIVSGMSQDHILVSC
                     VTREGEILNTLRRNLPNVQAVYVPHRTCGAFLAIIKIKKTAQGEPQQAIMAALGTEFY
                     TKYVIVVDEDVDIFDMSDVMWAISTRVRAEQDIVFIPGCKGAILDPTSDPETFTVTKM
                     GIDATRPVGKDFAERLAISDAQRARVRGILEASGVRL"
     gene            1221..2216
                     /locus_tag="VFY80_10480"
     CDS             1221..2216
                     /locus_tag="VFY80_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6008381.1"
                     /translation="MKIARIVMVMAAAGIGLGTLPAYAQQKVSLRFGTVGLGSAWYNY
                     GAGIADMVKPQLPAGSSIDVLPRAGGVGNIKLIQSNEMELGISFAVTSAEGCGGFGTF
                     KEKQNKVRALVGGLDIYYFGTFVTKKSGVTSWEDIAAAKNKFHLLTTRAGGTGEQGVR
                     QVLSLLGSSKEDIAKKGGQVEATERSGTAETIRDGQAHGWAHVVTKGHPAATQIVTVN
                     EMLVLPLPDKVIKGMVEKYGWAPAEIPANTFKGQTTPVKTVKAVSNILVSSDVPDAVA
                     YTITKTIIENADKLPKIHAALADFDPKKAAEPALNGNCPLHPGAAKYYKEARLLK"
     gene            2253..>2685
                     /locus_tag="VFY80_10485"
     CDS             2253..>2685
                     /locus_tag="VFY80_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008382.1"
                     /translation="MVALSPAHARLFLGFIAIALTAVEFTPLIRGRALYEITFLGIHV
                     GATTAITFLIIGFRARRKEDDSVPLYDVVLTLLALACAVYFAFQGYRVSERIEGVDEV
                     FFWDQVFGTLLVLLLLEACRRVAGIVLMVLAALFIAYVFLGP"
BASE COUNT          517 a          853 c          838 g          477 t
ORIGIN      
        1 gcgctgccag aaatacattc cctgcgaagt cgtcaagaac gcgccgtggc aggcggtgac
       61 gctcgaaggc gatgaggtgg acctcgccaa gctgccgatc ccgctccact tttcggtgga
      121 tgcggcgccc tacatcacag cgggccagat cgtcgcgcgc gatccggtga ccggcgtcga
      181 taccacggga tttcaccggc tgatggtcaa gggccggaac cggctcggcg tgtcgctgca
      241 ctcgcgccga cgcatgcacg agtttcaccg ccgggcggcg gagcgcgggc agaacctgcc
      301 ggcggcgatc acactcggca tccatcccct gcactacatg ggcgcgatgg tctacgccta
      361 cccgccacac gtgcgcaagt acgaaatcat cggcggtctc ttcggcgaac cgtaccagct
      421 cgccagcacc ggcttcaacg atctcgaggt gccgctcggc gcggagatcg tgatcgaggg
      481 cgaaatactg gccgacgtgc acgagccgga aggcccgttc agcgagttta ccggctacgc
      541 gtcctaccgc agcaccgaga acgtgttcgt cgtgaagcgc atcaggatgc gccgggatgc
      601 gatgtaccac tccatcgtct cgggcatgtc gcaggaccac attctggtgt cgtgcgtgac
      661 ccgcgagggt gaaatcctca acacgctgcg ccgcaatctt ccgaacgtgc aggccgtgta
      721 cgtgccgcat cgcacctgcg gcgcctttct cgccattatc aaaatcaaga agacggcgca
      781 gggcgagccg cagcaggcga tcatggcggc gcttggcacc gagttctaca ccaagtacgt
      841 gatcgtggtc gacgaggacg tcgacatctt cgacatgagc gacgtcatgt gggcgatctc
      901 gacacgggtg cgcgccgagc aggacatcgt gttcattccg ggctgcaagg gtgcgatcct
      961 ggatcccacc tcggaccccg aaaccttcac tgtcacaaaa atgggcatcg atgcgacgcg
     1021 cccggtcggc aaggacttcg cagagcggct cgcgatctcc gatgcgcagc gcgcccgcgt
     1081 tcgcggcata ctggaagcat ccggagtgag gctctaacgc aataccgtag tcaatcgaag
     1141 tcaatctttg tcatcccggc ggaatccaga atcctgagat gccggttttc accggcatga
     1201 cgatcgacaa ggaggattct atgaagatcg ctcgtatcgt gatggtgatg gccgcggccg
     1261 gcatcgggct gggtacgctg cctgcttacg cgcagcagaa ggtgagcctt cgattcggaa
     1321 ccgtcgggct gggatccgcg tggtacaact acggcgcggg catcgccgac atggtgaagc
     1381 cacagcttcc tgccggctcc tccatcgacg tgctgccgcg tgcgggcggt gtcggcaata
     1441 tcaagctcat ccagtccaat gaaatggaac tcggcatcag ctttgccgtg acttctgccg
     1501 aaggatgcgg cggcttcggc accttcaagg aaaagcagaa caaggtgcgg gcgctggtgg
     1561 gcgggctcga catctattac ttcggcacct tcgtcacgaa gaaatcgggt gtcacctcat
     1621 gggaagacat tgcggcggca aagaacaagt tccacctgct caccacgcgt gccggcggca
     1681 ccggtgagca gggagtacgg caggtgctgt cgctgctcgg ctcgagcaag gaagatatcg
     1741 ccaagaaagg cggacaggtc gaggccacgg agcgttccgg taccgccgag acgattcgcg
     1801 acgggcaggc gcacggctgg gcgcacgtgg tgacgaaagg ccatcccgcc gcgacgcaga
     1861 tcgtgacggt gaacgagatg ctcgtcctgc cactgcccga caaagtcatc aagggcatgg
     1921 tggagaagta cggctgggcg ccggccgaga tacccgccaa cacgttcaag ggccagacca
     1981 cgcctgtgaa aaccgtgaaa gcggtctcca acatcctggt cagctccgac gtgccggacg
     2041 cggtcgccta caccatcacc aagaccatca tcgagaacgc cgacaagctg ccgaaaatcc
     2101 atgccgcact cgccgatttc gatccgaaga aagcggccga gccggcgctg aacggcaact
     2161 gcccgctgca tccgggcgcc gcgaagtact ataaggaagc ccgtctcctc aagtaacgcc
     2221 gcagaagtct agaagccggg gagtacaggc tggtggtggc gctgtcgccc gcccacgctc
     2281 gcctgttcct cggcttcatc gcgatcgccc tcacggcggt cgagttcacg ccgctcattc
     2341 gcgggcgggc gctgtacgaa atcacttttc tcggcatcca cgtcggcgcc acaacggcga
     2401 tcacgttcct gatcatcggc tttcgcgcgc ggcgcaagga agacgactcc gtcccgctgt
     2461 acgacgtcgt gctgacgttg ctcgcgctcg cctgcgccgt ctatttcgct ttccagggct
     2521 accgtgtttc cgagcgcatc gagggcgtgg acgaagtctt cttctgggat caggtgttcg
     2581 gcaccctgct ggtcctgctg ctgctcgaag cctgccgccg ggtcgccggc atcgtgctca
     2641 tggtccttgc cgcgcttttc atcgcctacg tgttcctcgg gccgt
//
LOCUS       DASURG010000580         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_200488, whole genome shotgun sequence.
ACCESSION   DASURG010000580 DASURG010000000
VERSION     DASURG010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200488"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /gene="fusA"
                     /locus_tag="VFY80_10490"
     CDS             <1..1464
                     /gene="fusA"
                     /locus_tag="VFY80_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6008383.1"
                     /translation="QVVEVDEALMEKYLDQGEIEAGELHAPFEKALREGHLIPICFVS
                     ARNGAGVAELLDIFVRLMPNPTEGNPPQMLKGEGAGAQPIRAEPDPSKHVIAHVFKVV
                     IDPFVGKVGAFRVHQGTITRDSQLFVGDGRKPFKVGHLFLLQGKEFIETDALVPGDIG
                     AVAKVDEIHFDAVLHDSHDEDQIHLVPLEFPTPMHSLAIEPKRRGDEQRISEALHKLA
                     AEDPTFRVEHHSASNETVISGLGDLHMRYILDRMASQYHVEVTTKPPRIPYRETITAK
                     AEGHHRHKKQTGGAGQFGEVFLRIEPLKRGSGFEFVDQVKGGTIPTQFIPAVEKGVRQ
                     VLDSGPVAGFPVQDVRVIVYDGKHHPVDSKEVAFIAAGKRAFMDAIGKAKPIILEPIV
                     NIDINAPEGNMGDIAGDISAKRGQISGTDSTQAGMLSIRGQVPLAELNNYQARLKSVT
                     AGQGSFSIELSHYEPVPPNIQKDLAVQHAKAAHAEEE"
     gene            1469..1639
                     /locus_tag="VFY80_10495"
     CDS             1469..1639
                     /locus_tag="VFY80_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008384.1"
                     /translation="MRDLPAPRGVTAWGEHVDEIALRGMAKWPNVPARRELVSPGPVC
                     GKRRMARRLGKV"
     gene            1643..>2192
                     /locus_tag="VFY80_10500"
     CDS             1643..>2192
                     /locus_tag="VFY80_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX6008385.1"
                     /translation="MDEIVLRGMAKWPNVPAVYGWLSLDRRGNWRLKGEPISNPALNA
                     FIGRNYARDETGCWYFQNGPQRVFVTLEYTPYVYRVVSRPDAPLAFESHTGAPATSLR
                     GAWLDEAGSLLLETEHGPGLVHDRDLDRMFPLLVDANGAALDETVLENLMDALARGED
                     VPLWLQVRETSVRVAPISSDEVP"
BASE COUNT          433 a          687 c          716 g          356 t
ORIGIN      
        1 caggtcgtgg aggtggacga ggcgttgatg gagaaatacc tcgaccaggg tgagatcgag
       61 gccggggagt tgcatgcccc gttcgagaaa gcgctgcgcg agggacacct catccccatc
      121 tgcttcgtat ctgcgcgtaa cggagccggc gtcgctgagc tgctcgacat tttcgtgcga
      181 ctgatgccca acccgaccga aggcaatccg ccacagatgc tgaaagggga aggcgcgggg
      241 gcgcaaccga tccgggccga accggaccct tccaagcacg tcatcgcgca cgtcttcaag
      301 gtcgtgatcg acccctttgt cggcaaggta ggcgcctttc gcgtgcacca gggcaccatc
      361 acccgcgaca gccagctttt cgttggagac gggagaaagc cgttcaaggt cggacatctc
      421 tttcttcttc agggcaagga attcatcgag accgatgcgc tcgtcccggg cgacatcgga
      481 gcggtcgcga aggtggacga gatccacttc gatgccgtcc tgcacgactc gcacgacgag
      541 gaccagatcc atttagtgcc gctcgagttc ccgacgccga tgcacagcct cgcgatcgag
      601 ccgaagcgcc gcggcgacga gcagaggatc tcggaagctc tgcacaagct cgccgcggag
      661 gacccgacat tccgcgtcga gcatcacagc gcttccaacg agacggtgat cagcgggctc
      721 ggtgacctgc acatgcgcta catcctcgat cgcatggcga gccagtacca tgtcgaagtg
      781 acgaccaagc cgccgcgcat cccctaccgc gagacgatca ccgccaaggc ggaaggacat
      841 caccgccaca aaaagcagac cggcggcgca ggccagttcg gagaagtgtt cctgcgcata
      901 gagccgctta agcgcggctc cggtttcgaa ttcgtggatc aggtaaaggg cggcaccatc
      961 cccacccagt tcattccggc ggtggaaaag ggcgtgcgtc aggtgctcga ctccgggccc
     1021 gtcgccggat ttccggtgca ggacgtgcgt gtcatcgtat acgacggcaa acaccacccg
     1081 gtcgattcga aggaggtcgc gttcatcgct gcgggaaaac gagcgttcat ggacgcgatt
     1141 ggcaaggcga agccgatcat tctcgaaccg atcgtcaaca tcgacatcaa cgcgccggaa
     1201 gggaatatgg gcgacatcgc cggcgacatc tcggcgaagc gcgggcagat cagcggaacc
     1261 gattcaacgc aggcgggcat gctttcgatc aggggacagg tgccgctggc cgagctcaac
     1321 aattaccagg cgcggctcaa gtccgtgacg gcgggacagg gctccttctc catcgagctc
     1381 tcgcattacg agccggtgcc gccgaacatt cagaaggacc tcgccgtgca gcatgccaag
     1441 gccgctcacg ccgaagaaga atagcggtgt gcgcgatctt cctgcgccgc gtggggtgac
     1501 cgcgtggggt gagcacgtgg atgagatcgc gctgcgcggg atggcgaagt ggcccaacgt
     1561 gccggcgcgc cgcgaactcg ttagccctgg gcccgtttgc gggaagcggc gcatggcccg
     1621 ccggctcggg aaggtgtgag ccgtggatga aatcgtgctg cgcgggatgg cgaagtggcc
     1681 gaacgtgccg gcggtctacg gatggctctc gctcgaccgc cgcggaaact ggcggctcaa
     1741 aggcgagccc atcagcaatc cggccttgaa cgctttcatc ggccgcaact acgcacgcga
     1801 cgagaccggg tgctggtact ttcaaaacgg accccagcgc gtcttcgtca ccctggaata
     1861 cactccgtac gtctaccgcg tcgtgagccg cccggatgca ccgctcgcat tcgagtccca
     1921 taccggcgcg ccggcgacga gcctgcgggg cgcatggctg gacgaagcgg gcagcctgct
     1981 cctcgagacg gaacacggcc cgggcttggt gcatgaccgc gatctcgacc gtatgtttcc
     2041 tcttcttgtc gacgccaacg gcgccgcact cgatgaaacg gtgctcgaga atctgatgga
     2101 cgcgcttgcg cggggcgaag acgttccgct gtggctccag gtgcgcgaga cgagcgtgcg
     2161 ggtcgcgccg atcagctctg acgaagtgcc gc
//
LOCUS       DASURG010000581         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1324761, whole genome shotgun sequence.
ACCESSION   DASURG010000581 DASURG010000000
VERSION     DASURG010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324761"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFY80_10505"
     CDS             <1..321
                     /locus_tag="VFY80_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008386.1"
                     /translation="HCLNNGRRHERRAGIVEMDATRFSRGCICTPLGEERMHGLGMVR
                     KSRALCKGLRTATCKQRLAQGDAEPTVRRAAEFASASQVAIRGSPFAFVGQSLKHMAH
                     EFHG"
     gene            305..1297
                     /locus_tag="VFY80_10510"
     CDS             305..1297
                     /locus_tag="VFY80_10510"
                     /inference="COORDINATES: protein motif:HMM:NF008218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX6008387.1"
                     /translation="MSFTAEPFRAPWWLHNPHAQTIGARLLRRRRPNGIRRERVDTPD
                     GDFLDLDFSGDFSNPRAVVLLQHGLEGSALRGYALNLYHELRRYGIAAVGLNFRSCSG
                     ELNRVQRLYHSGETEDTRFVVQLLHERFPQARLGAIGFSLGGNALLKYLGEEGTATRL
                     AGAVAVSVPYDLGAGADYLEQSVMGRFYTRIFVKALVQKSRDKAEMIASACDFERGLR
                     ARTFREFDDAITAPLHGFAHADDYYERSSSAQFLPHIRIPTLLLHAEDDPFLPATSIP
                     HGNIAGNAQLQAIISKTGGHVGFIEGTPLRPRFWAEETAARFLAACLRPDAHQQ"
     gene            complement(1218..2147)
                     /locus_tag="VFY80_10515"
     CDS             complement(1218..2147)
                     /locus_tag="VFY80_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6008388.1"
                     /translation="MSVRGGVWVAFGAVYLIWGSTYLAIGKTVEDVPPVFLVAVRGLI
                     AGGVLYAWARWRGAVAVKARELVAMVPTATLLFGGGYVLVAWAEQEVPSGAAALLNAT
                     TPGWVVLFEWLTHKRARPTLRFAFAVGLGLGGVALLVGGRAQGAVPLLPALSIVLASV
                     AWGAGTLRARLTAHGDPVRNASVQLLTGGLLLLPLSLMLGEGTDIARGFATSSLLALA
                     YLTVFGSIVAYTAYVWLLHHVSAAKVSSHAYVNPLFAVVIGALLANEPIYATTLIAAA
                     FILISVFFIVGERPAASKPRETVQQSPRPRIAA"
     gene            2350..>2828
                     /locus_tag="VFY80_10520"
     CDS             2350..>2828
                     /locus_tag="VFY80_10520"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX6008389.1"
                     /translation="MVTFEMIGGRTCLDFANTASQRREGPFKERLESYEDLLSWAVQA
                     EQLSEAEAADLRRLAGARPSDAEATLGRARALREAIYRVFTARASDMELPAEDLKLIS
                     TEYGRAAINRVLTPSNVGVCSFEWQTHESLDRPLWPVAVSATNLVASEDALRVKECAT
                     "
BASE COUNT          548 a          929 c          885 g          466 t
ORIGIN      
        1 cactgcttga acaacgggcg tcggcatgag cgccgcgccg gcattgttga gatggatgcg
       61 acccgcttca gccgcgggtg tatctgcacg ccattggggg aggagaggat gcatggtctt
      121 gggatggtga ggaaaagccg ggctttgtgc aagggactgc gcactgcgac gtgcaagcag
      181 cgacttgcac aaggcgacgc tgaaccgacc gtcaggcggg ccgccgaatt cgcttcagcg
      241 tcgcaggtcg caattcgcgg gtcgccgttt gcttttgtcg gacaaagctt gaagcatatg
      301 gcccatgagt ttcacggctg agcccttccg tgcgccctgg tggctgcaca atccgcacgc
      361 acaaacgatc ggtgcccggc tcctgcgccg acgccgaccg aacggcatcc ggcgcgagcg
      421 cgtggacacg ccggacggcg atttcctgga tctcgacttc agtggcgatt tctcgaaccc
      481 gcgtgcggtg gtgttgctgc aacacggatt ggaaggctcc gcgctgcgcg gctatgcact
      541 caacctctat cacgagctga ggcgctacgg cattgccgcc gtcggtttga actttcgctc
      601 ctgtagtggt gagctgaacc gcgtacagcg gctgtatcac tccggcgaga ccgaggatac
      661 gcgcttcgtc gtacagctgc tgcatgagcg ctttccgcaa gcgcgcctgg gcgcgatcgg
      721 cttttcgctg ggtgggaacg cgctgctcaa gtatctcggc gaggaaggca cagcgacccg
      781 gctcgcgggc gccgtcgcgg tctcggtgcc ctacgacctg ggcgcgggtg cggattacct
      841 cgagcagtcg gttatgggac ggttctacac gcgcatcttc gtcaaggcgc tggtgcagaa
      901 aagtcgcgac aaggcggaga tgatagcgag tgcctgtgac ttcgagagag ggttacgcgc
      961 acgaacgttt cgcgagttcg atgatgcgat taccgcaccg ctgcacggct tcgcgcacgc
     1021 agacgattat tacgaacgct ccagctccgc gcagttcctg ccgcacatcc gcatcccgac
     1081 gctgctgctg catgccgagg atgatccgtt tctgccggcg acatcgattc cccatggcaa
     1141 tatcgccggc aatgcgcagt tacaggccat catctcgaaa acgggtggac acgtcggctt
     1201 catcgagggc acacccctca ggccgcgatt ctgggccgag gagactgctg cacggtttct
     1261 cgcggcttgc ttgcggccgg acgctcacca acaatgaaga agacgctgat cagaatgaac
     1321 gcggccgcga taagcgtcgt cgcataaatc ggttcgttcg cgagcagcgc gccaatcact
     1381 acggcaaaga gcggattcac gtacgcatgg ctcgagacct tggctgcact gacgtgatgc
     1441 agcagccaca cataagccgt gtaagccacg atcgagccga acaccgtcag gtacgcgagc
     1501 gcaagcagag acgacgtcgc aaatccacgc gcgatgtccg taccttcacc cagcatgagg
     1561 ctgagcggca gcaacagcag accgcccgtc agcaactgca cactcgcgtt gcgcaccgga
     1621 tcaccgtgcg ccgtcaggcg agcgcgtagc gtgcccgcac cccaggcaac gctggcgagc
     1681 acgatgctca gcgccggcaa cagcggcact gcgccttgtg cgcggccacc gaccagcagc
     1741 gccaccccgc ccagaccgag cccgaccgca aaggcaaaac gcagcgtggg tcgcgcacgc
     1801 ttgtgcgtca gccactcgaa cagtacaacc cagccaggcg tggtcgcatt cagcagcgct
     1861 gcggcccccg acggcacttc ctgctcggcc cacgccacca gcacgtaacc gccgccgaaa
     1921 agcagggtag ccgtcggcac catggccacc agttccctcg cctttaccgc caccgccccg
     1981 cgccaacggg cccaggcgta gagcacgcct cccgcaatca gcccgcgaac cgccaccagg
     2041 aagaccggcg gcacgtcctc cacggtcttg ccgatcgcca ggtaggtcga gccccagatg
     2101 aggtagaccg ccccgaaggc aacccacacc ccgcctcgta cgctcatgcc cgcttctccc
     2161 acggccgcgc cccgaccagg tcttctgccc gaccgccagc cacggccgcg aacctctgcc
     2221 agccattcaa ccaccaccga aacaccgact tcactgtagc cacaccccac ctccattgat
     2281 tttaccagca aaccgcgtta tggtggttac acagtaaatg gaggaatcta acctgtcaag
     2341 agtcttttga tggttacttt tgagatgatc ggcgggcgga cgtgcctgga tttcgcgaac
     2401 acggcgagcc agcggcgcga ggggccgttc aaggagaggc tcgagtcgta tgaggacctg
     2461 ctgagctggg ccgtgcaggc cgagcagctg agtgaggcgg aagcggcgga tctacgccgg
     2521 ctggccggcg cacgcccctc ggacgccgag gcgacgctgg ggcgagcgcg cgcgctgcgg
     2581 gaagcgattt atcgggtctt cacggcgcgc gcgtcggaca tggaattgcc cgccgaagat
     2641 ctgaaactga tcagcacgga gtacggccgg gccgcgatca atcgggtact caccccatcg
     2701 aacgtgggtg tgtgcagctt cgagtggcag acgcacgaat cgctggaccg tccgctctgg
     2761 ccggtcgcgg tctcggcgac caacctggtt gcatcggaag acgcgctgcg ggtaaaggag
     2821 tgcgcgac
//
LOCUS       DASURG010000582         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1120141, whole genome shotgun sequence.
ACCESSION   DASURG010000582 DASURG010000000
VERSION     DASURG010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120141"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFY80_10525"
     CDS             <1..352
                     /locus_tag="VFY80_10525"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6008390.1"
                     /translation="CHCAAVRYRIETELETATECNCSICTRKGVINHRVAPERLSILQ
                     GQEALGLYQFGTHTAKHWYCKHCGIHVFSNPRRAPDQYAVNLRTLDDFHRLRGGIEIR
                     HFDGQHWEQAVLRG"
     gene            complement(438..818)
                     /locus_tag="VFY80_10530"
     CDS             complement(438..818)
                     /locus_tag="VFY80_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6008391.1"
                     /translation="MSQSQVRTYAAQARSTDTFGRVLCTARNHHFVVDGPVQNGCPGE
                     AVNPGELFLAGVAACGVELVQVIAKERQVTPSAIAVDIEGMMDRSRPVRPDVSLFNSV
                     ALRFRLSGVTQAQAEDLIDRFRAR"
     gene            complement(854..1897)
                     /locus_tag="VFY80_10535"
     CDS             complement(854..1897)
                     /locus_tag="VFY80_10535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX6008392.1"
                     /translation="MRHTLALLGDINLMKVSDATVPFRRVREELAAADCRFANLECCL
                     YEQATQHELRDEGFFAPPPIGQALKLLGMDAIGNANNVNYGAEAIRSSCATLQALGIP
                     STGAGENREAAYAPAVVERKGVRYGFLQRSSVYWPTNHEAGESSPGIAVLQGHTAYQP
                     MLYKIRPELPPANRPGVPPAIITWADPRSLARYKEDVAALRARCDILTVSHHWGLFEE
                     VLDYQIEIAHAAIETGADAVIGHGPHYSLAMEMYKGKPVFYGLGNFSFHTGHGGRKHG
                     DWVGEAVRLTYEDKTLKRVAFKLVRHTEANETFFSQPKDEVQAIARLQSFCDRFGTRL
                     TVEGEELVVWKTA"
BASE COUNT          326 a          678 c          640 g          364 t
ORIGIN      
        1 ttgtcattgt gcggcggtgc gctatcggat cgaaaccgag ctcgagaccg cgaccgagtg
       61 caattgctcg atctgcacca ggaagggcgt gatcaaccat cgcgtcgccc cggagcggct
      121 gagcatcctg cagggccagg aagcgctggg cctttaccag ttcggaacgc acaccgccaa
      181 gcactggtac tgcaagcact gcggcattca cgtcttcagc aatccgcgcc gtgcgcccga
      241 ccagtatgcc gtcaacctgc gtacgctgga cgattttcac cgcctgcgcg gcggcatcga
      301 gatccgccac ttcgacggcc agcattggga acaggcggtg ctgcgcggct gagcgcgcgc
      361 agtcgcaaac ccgatcaagc aacgctcacg tcgaccttca catccggcgt cgcaactgcg
      421 acgctgccgt agagcggtca cctcgccctg aagcggtcga tcagatcctc cgcctgcgct
      481 tgggtcaccc cgctcagccg gaagcgcaat gcgaccgaat tgaacaagct gacgtccggc
      541 cgtaccggcc tggagcgatc catcatgcct tcaatgtcga ccgcgatcgc gctcggcgtg
      601 acctgccgct ccttggcgat gacctgcacg agctccacgc cgcaggcggc cacaccggcg
      661 aggaagagct cgcccggatt gaccgcctcg ccgggacagc cgttctgcac cggaccgtcg
      721 acgacgaagt gatgattgcg tgcggtgcag agcacccggc cgaaggtgtc ggtcgagcgc
      781 gcctgcgcag cataggtgcg cacctggctt tgactcatcg ttgcacccgt ttcgctggtt
      841 gccaaagaat cgttcaggcc gtcttccaga cgaccagctc ctcgccctcc accgtgagcc
      901 tggtgccgaa ccgatcgcag aacgactgca gccgcgcgat cgcctgcacc tcgtccttcg
      961 gctgcgagaa gaacgtttcg ttcgcttcgg tgtgccgcac cagcttgaac gcgacgcgct
     1021 tgagggtctt gtcttcgtag gtgagccgca ctgcctcgcc cacccagtcg ccgtgcttgc
     1081 ggccgccgtg accggtgtgg aacgagaagt tgcccagccc gtagaacacg ggcttgccct
     1141 tgtacatctc catggcgagc gaatagtgcg gtccgtggcc gatcacggcg tcggccccgg
     1201 tttcgatcgc cgcgtgcgcg atctcgatct ggtagtcgag cacttcctcg aacagtcccc
     1261 agtggtgcga gacggtgagg atgtcgcagc gcgcgcgcag cgcggcgacg tcctccttgt
     1321 agcgcgcgag cgacctggga tcggcccagg tgatgatcgc gggcgggaca ccgggacggt
     1381 tcgcgggcgg aagctccggc cggatcttgt acagcatcgg ctggtacgcg gtatggcctt
     1441 gcagcaccgc gatccccggc gacgattcgc ccgcctcgtg gttggtgggc cagtagacgg
     1501 agctccgctg caggaacccg tagcgcacgc ccttgcgctc gaccaccgcc ggcgcatagg
     1561 ccgcttcccg gttctcgccc gcgccggtgc tcggaatgcc gagcgcctgc agcgttgcgc
     1621 acgaggagcg gatcgcttcc gctccgtagt tcacgttgtt ggcgttgccg atcgcatcca
     1681 tgcccaggag cttgagcgcc tggccgatcg gcggcggggc gaagaagcct tcgtcgcgca
     1741 gctcgtgctg ggtggcctgc tcgtagaggc agcattccag gttggcgaag cggcagtccg
     1801 cagcggccag ctcttcgcgc acgcgccgga acggcacggt ggcgtcgctc accttcatca
     1861 ggttgatgtc gcccagcagg gccaatgtgt gtctcacgct tgctctccca cgctattgag
     1921 gattcgcaaa atggctgtcc tccattctgc gctcgacgct ccccgcttcc ccgggagagc
     1981 aaccgtgttc aaggtcaagt ggtttttg
//
LOCUS       DASURG010000583         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_813642, whole genome shotgun sequence.
ACCESSION   DASURG010000583 DASURG010000000
VERSION     DASURG010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813642"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFY80_10540"
     CDS             <1..608
                     /locus_tag="VFY80_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX6008393.1"
                     /translation="ALFDAYARANTILVNLDRDIWGYISLGYFRQKVKPGEIGSSTMP
                     HKVNPIDFENSEGNLGVANALFRHMSEKLPISRWQRDLTDSTVLRNMGVALGHTLLGY
                     DSCSRGIAKLEANPDRLREDLDANWEVLAEPIQTVMRRHGIKDAYEQLKALTRGKAGI
                     TRESLHEFIQRLPIPEKERQRLLKLTPAGYTGKAAQLARRI"
     gene            complement(626..>2275)
                     /locus_tag="VFY80_10545"
     CDS             complement(626..>2275)
                     /locus_tag="VFY80_10545"
                     /inference="COORDINATES: protein motif:HMM:NF003714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX6008394.1"
                     /translation="FSARFGTPILLIFLVLGMLAGEDGPGGIEFNDFQLAYLFSTLAL
                     AVILLDGGMRTNAETFRVALKPAVSLATVGVVVSAAITGAFAALVFDLNWLHGLLVGA
                     IVGSTDAAAVFALLHAHGMELKQRVGATLEVESGSNDPMAVFLVVVLIEAIAAGQTML
                     SWTVLFEFAKQMTLGAVAGVAGGRLLVWANNRLSLETGLYPLLAIAGGVTIYGGVTVA
                     GGSGFLAIYLVGIVLGNAALQAKQNILRVNDGMAWLSQIMMFLMLGLLVTPSQIPPIA
                     LPAIGVGLALILVARPVAVWVSLLPFRFPWREQVFIAWVGLRGAVPIILGTFPLLAGV
                     ENAMSYFNITFIAVLISLVLQGWTLAPVARLLNLEVPPKARPLSYLDLDIPGQFNHDL
                     LGYQLETGSLAAGKHPSQLPLPQEVELAAVIRGTHVLKLSEIGALQAGDYVYVLAIPE
                     HAEALNRMFGVAHGPDRLEEHRFFGDFMLDGGARVADVEAVYSLGLDFRDPDETLAAF
                     LSRQFRGNPVVGDRLRAHGVELVVRAVERGIITRVGLKLHD"
BASE COUNT          490 a          717 c          713 g          355 t
ORIGIN      
        1 cggcgctctt cgatgcatac gcccgggcaa ataccatctt ggtcaacctc gaccgcgaca
       61 tctggggcta catctcgctc ggctacttca ggcagaaggt gaaaccgggc gagatcggct
      121 cgtccaccat gccgcacaag gtgaacccga tcgatttcga aaattccgaa ggcaatctcg
      181 gcgtggcgaa tgcgctgttc cggcacatga gcgagaagct gcccatctcg cgctggcagc
      241 gcgatctcac ggactccacg gtgctgcgga acatgggcgt cgcactgggc cacacgctac
      301 tcggctacga ctcctgctcg cggggaattg ccaagctcga agcgaacccg gacaggctgc
      361 gtgaggatct ggacgccaac tgggaagtgc tcgccgagcc gatccagacc gtgatgcggc
      421 gccacgggat caaggatgct tacgagcagc tgaaggcctt gacccggggc aaggcaggta
      481 tcacgcggga aagcctgcac gaatttatcc agcgtttgcc gattcccgag aaggaaagac
      541 agcgcctgct caaacttacg cctgcaggct ataccgggaa agcagcgcag ctcgcgcgca
      601 gaatctgaac gccttaaagc cggcgtcagt cgtgcagctt gaggccgact cgggtgatga
      661 tgcctctttc caccgcgcgc accacgagtt caacgccatg cgcgcgcagc cggtcaccta
      721 ccaccggatt gccgcggaac tgacgggaca ggaaggcggc gagcgtctcg tccggatcgc
      781 ggaagtcgag cccgagactg tagaccgctt cgacatccgc gacgcgggcg ccgccgtcca
      841 gcatgaaatc gccgaagaaa cggtgttctt cgaggcggtc cggcccgtgg gcgacgccga
      901 acattcgatt cagcgcctcg gcatgctccg gaatggcgag cacatagacg taatctcccg
      961 cctgcagcgc gccgatttcg ctcaacttca acacgtgcgt gccgcgtatg acagccgcaa
     1021 gctcgacttc ctgcggcaac ggaagctggg aaggatgctt gccggcggcg agactgcccg
     1081 tttccaactg gtagccgaga agatcgtgat tgaattgtcc ggggatatcg agatcgaggt
     1141 agctgagcgg cctcgccttg ggcggcactt cgagattcag cagccgggcg acgggcgcga
     1201 gcgtccatcc ctgaagcacg agcgatatca gcaccgcgat gaacgtgatg ttgaagtaag
     1261 acatcgcgtt ctcaacaccg gcaagcagag gaaaggtccc gaggatgatg ggtacggctc
     1321 cccggaggcc cacccaggcg atgaagacct gctcgcgcca cggaaagcgg aagggaagca
     1381 ggcttaccca caccgcgacc gggcgcgcga ccaggatcag cgcgagtccg accccgatag
     1441 cggggagcgc gatgggcggg atctgcgatg gggtaacgag gagccccagc atcaggaaca
     1501 tcatgatctg cgagagccag gccatcccgt cgttcacccg caagatattc tgtttcgctt
     1561 gcaacgcggc gttgcccagc acgatgccga cgaggtagat cgccagaaag ccgctgcctc
     1621 cggccacggt gacgccgcca tagatggtga cgccgcctgc aattgcgaga agcggataaa
     1681 ggccggtctc gaggctcaac cggttgttcg cccacaccag cagccgcccg cccgccacgc
     1741 cagccaccgc accgagcgtc atctgctttg cgaactcgaa gagcaccgtc cacgaaagca
     1801 tcgtctgtcc cgctgcgatc gcttcgatca gcacgacgac caagaagacc gccatcggat
     1861 cgttgctgcc cgactccact tcaagtgtcg cgccgacgcg ctgtttcagt tccatgccgt
     1921 gtgcgtggag caatgcaaat acggcggcgg catcggtgga tcccacgatg gcgccgacga
     1981 gtaacccgtg cagccagttc agatcaaaga ccaacgcggc gaacgcaccg gtgatcgcgg
     2041 cggacaccac tacacccacg gtggcgagcg atacggcggg tttcagcgct acgcgaaagg
     2101 tctcggcgtt cgttcgcatg ccaccgtcga gcagaatcac cgcaagcgcc agcgtgctga
     2161 agagatacgc cagctggaag tcgttgaact cgattccacc gggtccgtcc tcgccggcga
     2221 gcatgccgag cacgagaaag atgaggagga tcggggtgcc gaagcgcgcg gagaa
//
LOCUS       DASURG010000584         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1325586, whole genome shotgun sequence.
ACCESSION   DASURG010000584 DASURG010000000
VERSION     DASURG010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325586"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFY80_10550"
     CDS             complement(<1..1135)
                     /locus_tag="VFY80_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX6008395.1"
                     /translation="MPHRTTRTEHDLLGERSVPGAAYYGIHTLRALENFPITGTPISI
                     YPELVNALACVKQAAALANNELKLIDNDRTRAIVQACQEIRDGALLEEFVVDVIQGGA
                     GTSTNMNANEVIANRGLEILGRRKGEYRHLHPVDHVNMSQSTNDVYPTAVKVALHFAI
                     KRLTAAMAELRAAFLGKAAAFADVLKVGRTQLQDAVPMTLGQEFSTYAVMLEEDEQRL
                     KEAALLIHEINLGATAIGTGITTHPQYAALACRRLADITGIELVTASNLVEATQDAGA
                     FVQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGLYEINLPPVQAGSSIMPGKVNPVIPE
                     VVNQIAFEVIGNDVTVTFAAEAGQLQLNAFEPVIAHSLFKSLKH"
     gene            1304..2224
                     /locus_tag="VFY80_10555"
     CDS             1304..2224
                     /locus_tag="VFY80_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6008396.1"
                     /translation="MKRRALVLTAVLVACGFAASAAAAELTGTLKKIKDTGVVKIGHR
                     DASIPFSYLDDRQKPIGYGVDICMKVVDAIKQELKVKDLKVEFVPVTSQTRIPILTGG
                     NIDLECGSTTNSVERQKQVAFAYTYFFTGTKLLVKTNPGIKSYDELKGKTVVVTQGTT
                     NERAIKAYNDQKKLGMNFIHAKDHGESFLAVQTGRAVAFPMDDILLYGLRANAKNPAE
                     FAVVGEFLSEDPYGIMLRKDDAPFKKVVDSTLAQLYKSGEINKIYAKWFQSKIPPKGI
                     NLNLPMSDKLKELVKNPSDQGVGQCGMMKC"
     gene            2313..>2791
                     /locus_tag="VFY80_10560"
     CDS             2313..>2791
                     /locus_tag="VFY80_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6008397.1"
                     /translation="MDFSIFLQPAFSGGGTYLDWVVSGVKWTFWVSILAWIIAFSVGS
                     VLGVLRTMPLPLLRGFATGYVELFRNVPLLVQMFIWYFVMPELVPQQLGQWMKTGMPM
                     PEFWSAVLCLGFYTASRVAEQVRSGIQSIPRGQTYAALATGLTLPQTYRHVLLPMAYR
                     "
BASE COUNT          503 a          895 c          865 g          528 t
ORIGIN      
        1 gatgcttcag gctcttgaac aggctgtgcg cgatcaccgg ctcgaaggca ttgagctgca
       61 gctgcccggc ttcagcggca aaggtgaccg taacgtcgtt gccgatgact tcgaacgcga
      121 tctggttcac cacttccggt atgaccggat tcaccttccc cggcatgatg ctggagcccg
      181 cctgcaccgg cgggagattg atctcgtaga gccccgcgcg cggccctgac gagagcaggc
      241 gcaggtcgtt gcaggtcttg gagagcttga ccgcgacgcg cttcagcacg ccggagagct
      301 gcacgaaggc gcccgcgtcc tgcgtcgcct ccaccaggtt gctcgcggtg acgagctcga
      361 tgccggtgat atcggcaagg cgccggcatg cgagcgcggc atactgcgga tgagtggtga
      421 tgccggtgcc gatcgcagtc gcgccgagat tgatctcgtg gatcaggagc gccgcctcct
      481 tgagccgctg ctcgtcctcc tcgagcatga ccgcgtaggt ggagaattcc tgcccgagcg
      541 tcatcggcac ggcgtcctgg agctgcgtgc gccccacctt gagcacgtcg gcaaacgccg
      601 ccgcctttcc gagaaaagcc gcgcgcaatt ccgccatcgc cgcggtcagg cgcttgatcg
      661 cgaagtgcag cgcgaccttc accgcggtgg gatagacgtc gttcgtgctc tggctcatgt
      721 tgacatggtc gaccggatgc agatggcggt attcgccttt cctgcgcccc aggatctcga
      781 ggccgcggtt cgcgatcacc tcgttcgcgt tcatgttggt ggacgtgccg gcgccgccct
      841 ggattacatc gaccacgaac tcttcgagca gcgcgccgtc gcggatttcc tggcatgcct
      901 gcacgatggc gcgcgtcctg tcgttgtcga tgagcttcag ctcgttgttc gcaagcgccg
      961 ccgcctgctt gacgcaggca agcgcattca cgagctcggg atagatcgag atcggcgtcc
     1021 cggtgatggg aaagttctcc agcgcgcgca gcgtgtggat gccgtagtag gcggccccag
     1081 gcacgctgcg ctcgccgagg agatcgtgtt ccgttcgcgt cgtccggtgt ggcatggctc
     1141 gtctcgcggt tgactgagtc gagactatac caacacgcgg caatcgattg attagccaca
     1201 cgatgcggct ggacaaagcc atgcggttcg gctagattcc cgcacagcaa cgcagtcacc
     1261 gcggcccgtc gttcgagcgc cgcatcagag agaggagact tccatgaaac gccgcgcact
     1321 cgtcctgacg gccgtgctcg tcgcctgcgg ctttgctgct tccgccgccg ctgccgagct
     1381 gaccggcacc ctcaagaaga tcaaggatac cggggtcgtg aagatcggcc atcgcgacgc
     1441 ctcgatcccg ttctcgtatc tcgacgaccg gcagaagccg atcggatacg gagtcgatat
     1501 ctgcatgaag gtcgtggatg cgatcaagca ggaactcaag gtgaaggacc tcaaggtcga
     1561 gttcgtcccg gtcacctcgc aaacgcgcat ccccattctc accggcggaa acatcgatct
     1621 cgaatgcggc tccaccacca acagcgtgga gcgccagaag caggtcgcct tcgcctatac
     1681 ctatttcttc accggcacga aactcctcgt caagacgaac cccggcatca agagctacga
     1741 cgaactcaag ggcaagaccg tggtcgtcac gcagggcacg acgaacgagc gcgccatcaa
     1801 ggcgtataac gaccagaaga agctcggcat gaacttcatc cacgcgaagg atcacggcga
     1861 gtcgttcctg gccgtgcaga ccggccgcgc cgtggcattc ccgatggacg acatcctgct
     1921 ctacggcctg cgcgcgaatg caaagaaccc ggcggaattc gccgtcgtgg gggaattcct
     1981 ctcggaggat ccgtacggca tcatgctgcg caaggacgat gcgcccttca agaaagtcgt
     2041 tgattcgacc ctggcgcagc tctacaagag cggggagatc aacaagatct acgccaagtg
     2101 gtttcagtcc aagatcccgc ccaagggcat caacctgaac ctcccgatgt ccgacaagct
     2161 gaaggaactg gtcaagaacc cgagcgacca gggcgtcggg cagtgcggca tgatgaaatg
     2221 ctgacggcgt gaggcgcgcg cacgcatacg caatcgggga accgcggttc cccgattttt
     2281 ttggcgcccg cgctccgcgc taaacttccg cgatggattt cagcatcttc ctgcagcccg
     2341 ccttctcggg cggcggcacg tatctcgact gggtcgtctc tggagtcaag tggaccttct
     2401 gggtgtcgat cctcgcgtgg attatcgcgt tcagcgtggg ctcggtgctc ggcgtgctgc
     2461 gcaccatgcc cctcccgctg ctgcgcggct ttgccaccgg ctacgtcgag ctctttcgca
     2521 acgttccgct gctggtgcag atgttcatct ggtacttcgt catgccggag ctcgtgccgc
     2581 agcagcttgg ccagtggatg aagaccggga tgccgatgcc ggaattctgg agcgccgtgc
     2641 tgtgcctggg gttctatacc gcctcgcgcg ttgccgagca ggtgcgctcc ggcatccagt
     2701 cgatcccgcg cggccagacc tatgcggcgc tggcgaccgg cctcacgctg ccgcagacat
     2761 atcgccacgt tctcctgcca atggcctatc g
//
LOCUS       DASURG010000585         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_814129, whole genome shotgun sequence.
ACCESSION   DASURG010000585 DASURG010000000
VERSION     DASURG010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814129"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..417)
                     /locus_tag="VFY80_10565"
     CDS             complement(127..417)
                     /locus_tag="VFY80_10565"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008398.1"
                     /translation="MRSSLVESGTRQADRLIAGLTDMAEKAQQLVREAGAHVDDTVDH
                     TRSKLVNAGAAVSESAQYAARATDAYVRDNPWKVLGFAAAVGAIVAILLTRR"
     gene            complement(556..810)
                     /locus_tag="VFY80_10570"
     CDS             complement(556..810)
                     /locus_tag="VFY80_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008399.1"
                     /translation="MTTQKEGELEQRRAEELKREFRYWWGTLSRDEILKLAAKRDELL
                     LLLHEKYGYAHHEAEEEIETALRSLDTRVHRDTPGDARGR"
     gene            complement(807..1895)
                     /locus_tag="VFY80_10575"
     CDS             complement(807..1895)
                     /locus_tag="VFY80_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6008400.1"
                     /translation="MNGPQDFPAWLDVLARVMNVGMLLIDPGGRPQFANRLALDLLGC
                     ASEPELHVRWPELQQGLGIDRETAIGRPPQRLTVDLPTRTGTRHLRLEIHARNEPECR
                     GCLILLRDRHAADLLETDLLLASRMRSLVHVYRVLAHDLKAPLNAMQLTLELLADSSA
                     YEAPDGEARRRRHIEVLREELSRLNRILQTMLDQKEPLDAVARLFDLREVIREIIVLL
                     APQARAQRVELMIDLPQAPTCIQGFRDRLKQALLNVAINGLEAMPGGGSLGIRLDASD
                     ADAVVRVSDTGPGISQEVQEEIDQIYFTTKGSGTSIGLYVARLVVESHGGEIAVESGS
                     GKGTEFTVRLPLRNETTASANVAAGGYT"
     gene            2219..2575
                     /locus_tag="VFY80_10580"
     CDS             2219..2575
                     /locus_tag="VFY80_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6008401.1"
                     /translation="MDDDMHALEILSEALRRAGAVVWQAVSAREALEQLARQLPDIVL
                     SDIGMPDEDGCVMLSGIRALPTAAGGALPAIAVTGYARPEDLAAARSAGYQAVVTKPV
                     EFGELSHVILELTGRA"
     gene            complement(2581..2787)
                     /locus_tag="VFY80_10585"
     CDS             complement(2581..2787)
                     /locus_tag="VFY80_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6008402.1"
                     /translation="MNWDRIQGNWKQFKGKAKEQWGKITDDDLDRIAGQREQLAGKIQ
                     EYYGIGKDEAERQLNDWERTLDRP"
     gene            complement(2810..>3362)
                     /locus_tag="VFY80_10590"
     CDS             complement(2810..>3362)
                     /locus_tag="VFY80_10590"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX6008403.1"
                     /translation="FPAWHELCSVRCMEFWSVDWGQLFVPSGPLLEIVVRGSVLYMAL
                     FFLLRILPRRQIGSLGVSDLLVVVVIADAAQNGMSGEYRSITEGLALVCTILFWNWVI
                     DTLDYRFPQLSINGTAPRLVVSKGRMLRQNMEKEELSEEQLMSHLRQQGVEDLRLVSK
                     AYVEGDGRISVIKASRPRAQSSS"
BASE COUNT          596 a         1107 c         1006 g          653 t
ORIGIN      
        1 catggctact tcgtcgccga cctgcgggac gcacgtccgg agcgccccgt gttcaaccgc
       61 gcggtctgcc tgcgcgattc atgggcgccg gcgcgcaagt agcgctcgcg cgccgagcaa
      121 acggcttcag cgacgcgtga gcagaatcgc gacgatggca ccgaccgccg cggcaaaacc
      181 cagcactttc cacggattgt ccctcacgta ggcgtccgtc gcacgggcag cgtattgcgc
      241 ggattcggac accgccgctc cggcgttcac gagtttggac cttgtgtggt ccaccgtgtc
      301 gtccacatgc gcccctgctt cgcgcacgag ctgctgcgct ttttccgcca tgtcagtcaa
      361 gccggcgatc agcctgtccg cttgccgcgt acccgattca acgagactac ttctcatagc
      421 cgactcctaa agagtgaatg caacacgagg ttcagcaagt ttcatgccgc caccgcgagt
      481 agggcttccc atgcgaaacg catgttgtct gcgcggagac cctgcgttgc aggaattgcg
      541 cagcgcgtgt aagagttacc ggccacgcgc gtcgcccggt gtgtcacggt gaacccgggt
      601 atcgagcgag cgcagggcag tttcgatttc ctcctcggct tcgtgatgcg catacccgta
      661 cttctcgtgc agcagcagaa gcagttcgtc gcgcttcgcc gcaagcttca ggatctcgtc
      721 gcggctgagc gtcccccacc aataacggaa ttcgcgcttg agctcctctg cgcggcgctg
      781 ctccagttcg ccttccttct gcgtcgtcac gtatagcctc ctgccgcgac attggcgctc
      841 gcggtcgtct cgtttctgag gggcaagcgc actgtgaatt ccgtgccctt gccgcttcca
      901 ctttccaccg cgatctcgcc accgtgcgac tcgaccacca gtcgcgcaac gtagagcccg
      961 atgctggtcc cgctaccttt ggtcgtgaaa taaatctggt cgatctcctc ttgcacttcc
     1021 tgagatatcc cgggtcccgt atcgcttacg cgcacaaccg cgtccgcatc cgaagcgtcg
     1081 aggcggattc cgagagagcc tccgcccggc atcgcctcca gcccgttgat cgcaacgttg
     1141 agcaacgcct gcttgaggcg atctcgaaag ccctgaatgc atgtgggcgc ttgaggcaaa
     1201 tcgatcatga gctcgacgcg ctgcgcgcgc gcctgtggag cgagcagcac gatgatttca
     1261 cggatcacct cacgcaggtc gaagagacgc gccacggcat ccagcggctc cttctgatcg
     1321 agcatggtct gcagaatgcg gttgagcctc gacaactcct cgcgcagcac ttcgatgtgg
     1381 cgccggcggc gggcctcgcc gtcgggcgct tcgtaggcgc tcgagtccgc cagcaactcg
     1441 agcgtcagct gcatcgcgtt gagcggtgcc ttgagatcgt gagccagcac acggtagaca
     1501 tgaacaagtg aacgcatgcg gctagcaaga aggagatcgg tctcgaggag gtccgctgca
     1561 tggcgatcgc gcagcaggat caggcagcct cggcactcgg gctcgtttcg ggcatggatt
     1621 tcgagccgca gatgacgcgt cccggtccgc gtgggcaggt caacggtgag gcgttgaggt
     1681 ggacgcccga ttgcggtctc tcgatcgatt cccaggcctt gctgcaattc cggccatcgc
     1741 acgtgcagtt ccggctcgct cgcgcaaccc agtagatcca gcgcgagacg gttggcaaac
     1801 tgaggccgcc caccagggtc gatcagcagc atgccgacgt tcatcacgcg cgccaagacg
     1861 tcgagccatg cgggaaaatc ctggggaccg ttcatgcttg ccgcatccaa attgtcctcg
     1921 gcaacccacc ttattccgac agtcggacat gtctcttaga tcatgggaag cgccgaggtt
     1981 ccgcgcggca gtctcgtgcg ccgctcgcca ttggcacgct tgctgcttcc cgcgccacga
     2041 aacccagcca gaaaaagcgc tgtggtgcta tttaaagtga acgcatatgc aacgcatgcc
     2101 gactatgcgc tcgagcgagc gatccggacc ccgcatagat gaccgcctag aaaagacagt
     2161 gcggcggcag cggcccgagt ctaagcccgc tcccctcggc ggactctcga tactcgcggt
     2221 ggacgacgat atgcacgcgc tggaaatcct gagcgaggcg ctccgccgcg cgggcgcggt
     2281 ggtgtggcag gcggtttcgg cgcgggaggc gctcgagcag ttggcgcggc agcttcccga
     2341 tatcgtgctc tcggacatcg gcatgccgga cgaagacggc tgtgtcatgc tgtcgggtat
     2401 ccgcgccctc ccgacagcag cgggcggcgc gctccccgcc atcgcggtga cagggtatgc
     2461 acgccccgag gacctcgctg cggcccgctc agcgggttac caggccgttg tgacgaagcc
     2521 cgtggagttc ggcgaacttt cgcatgtgat cctcgaactc acgggccgcg cctgaaggcc
     2581 ttacggccgg tcgagggtgc gttcccaatc gttcagctga cgttccgcct cgtccttgcc
     2641 gatgccgtag tactcctgga tcttcccggc cagctgctca cgctggcccg caatgcggtc
     2701 aagatcatcg tccgtgatct tgccccactg ctccttcgcc ttgcccttga actgcttcca
     2761 gttgccttgt atgcgatccc agttcatgtc gactgctcct ttcggttggt taagaagacg
     2821 actgcgcccg cgggcgggag gctttgatga cgctgatgcg cccgtctccc tccacgtacg
     2881 ctttgctcac cagccgcaag tcctcgacgc cctgctggcg cagatgactc atcagctgtt
     2941 cttcggaaag ctcctccttc tccatgttct gccgcagcat gcgccctttg ctgacgacga
     3001 ggcgcggcgc cgtgccgttg atgctcaatt gtgggaaccg gtagtcgagg gtatcgatca
     3061 cccagttcca gaaaagaatg gtgcagacaa gcgccaagcc ttccgtgatg gagcggtact
     3121 cgcccgacat tccgttttga gccgcgtcgg ctatcaccac gacgaccagc agatcggata
     3181 cgccgagcga tccgatctgg cgccgcggta ggatacggag caggaaaaag agcgccatgt
     3241 agagcacgct tcctcgcacc acgatttcga gcaggggacc gctcggcacg aaaagctgtc
     3301 cccaatctac gctccaaaac tccatgcacc ggacgctgca aagttcatgc catgccggga
     3361 ag
//
LOCUS       DASURG010000586         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_406486, whole genome shotgun sequence.
ACCESSION   DASURG010000586 DASURG010000000
VERSION     DASURG010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406486"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /gene="rplF"
                     /locus_tag="VFY80_10595"
     CDS             <1..127
                     /gene="rplF"
                     /locus_tag="VFY80_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX6008404.1"
                     /translation="QKVGQVAAELREFRPPEPYKGKGIRYQGEQVRRKAGKTAGK"
     gene            127..510
                     /gene="rplR"
                     /locus_tag="VFY80_10600"
     CDS             127..510
                     /gene="rplR"
                     /locus_tag="VFY80_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX6008405.1"
                     /translation="MALRTKSKDREVLRTRRHARVRRKVAGTPERPRLVVHRSSKNMS
                     AQIADDIAGKVLLGVSTLAPELKDAREKDGATKKELAKAAGKLLAEKAKAAGITKVVF
                     DRGGYLYHGRIAAFAEGAREGGLEF"
     gene            683..1183
                     /gene="rpsE"
                     /locus_tag="VFY80_10605"
                     /pseudo
     CDS             683..1183
                     /gene="rpsE"
                     /locus_tag="VFY80_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            1173..1394
                     /gene="rpmD"
                     /locus_tag="VFY80_10610"
     CDS             1173..1394
                     /gene="rpmD"
                     /locus_tag="VFY80_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX6008406.1"
                     /translation="MASKKKAAGTPRRLKITQIRSANGRPETHRRTLRALGLKHQHSV
                     IHDENEAIKGMLFQVRHLVEVSEVEGEEK"
     gene            1395..1847
                     /gene="rplO"
                     /locus_tag="VFY80_10615"
     CDS             1395..1847
                     /gene="rplO"
                     /locus_tag="VFY80_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX6008407.1"
                     /translation="MSDLHNLRRPEGANRGKKRLGRGPGSGKGKTSGKGHKGQKARTG
                     HHGIPSWFEGGQMPLQRRVPKRGFKNPFRTVFEVVNVGQLNKLSDDTITPETMHSNRL
                     VDIGKGNPVKVLGDGEVTRKFTVRAHAISDSARAKIEAAGGTVELLEA"
     gene            1851..>2650
                     /locus_tag="VFY80_10620"
     CDS             1851..>2650
                     /locus_tag="VFY80_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6008408.1"
                     /translation="MANPIPNLFKIPELKEKILFTLLTLLIYRVGAHITAPGIDVGAL
                     REQFGQLQSSLFGVYDMFVGGALSRATIFGLGIMPYISASIMFQLLAAVVPAIEKMQK
                     EGEDGRKKLTQWTRYTTVGLALLQGYGYAVFVQSIPGAVRNPGFFFLLSTALILTIGA
                     VFVMWLGEQITERGIGNGMSLLIFFSIIESFPTQVANTWEQVATDVISPFAVILMVII
                     MVAVVAGVVAMTMAARKIPVQIPRKVMGRGRVREGQKSFIPLRINSSGV"
BASE COUNT          548 a          758 c          892 g          452 t
ORIGIN      
        1 gcagaaggtc ggccaggtcg cagccgagct gcgcgagttc cgcccacccg agccgtacaa
       61 gggcaagggt atccgttatc agggcgagca ggtccggcgt aaggccggca agacggcagg
      121 taagtaatgg cactgagaac aaaatcaaaa gaccgcgaag tactgcgcac gcgccgccac
      181 gcccgtgtcc gccgcaaagt ggccggcaca ccggagcgcc cgcgtctggt cgtacatcgc
      241 tcttccaaga acatgtcggc acaaatcgcc gacgatatcg ccggaaaagt actgcttggc
      301 gtctccaccc tcgcgcccga actgaaggat gcgcgcgaga aggacggagc aaccaagaag
      361 gaactggcca aggcagcggg caagctgctg gcggaaaaag ctaaagcggc cggcatcacg
      421 aaggtggtgt tcgaccgcgg tggttacctg tatcacggcc gtatcgccgc gttcgccgaa
      481 ggcgcacgcg agggcgggct ggagttctaa tccgattatg gaaaatagaa atgcaagagg
      541 cccgcagcgc gggcgtggtg gtccgggtcg cggcggcagc gaaggcgggg ccaatcccgg
      601 cggcgaccgt ggcggtgatc gcggacgcgg tggccgcgac ggcggcgata agcggcgtga
      661 acgccaggat agcgatcagg tagaaaacgt gatcttcgtc aaccgcgtcg ccaaagtagt
      721 aaagggcgga cgtcgcttct ctttcaccgc gctggtcgcg gtgggcgatc gcaaaggcaa
      781 tgtcggcatc gcgctgggca aggcgaacga ggtttccgaa gcgattcgca agggctttga
      841 acaggcgcgt cgcgcgatgg tgtcggtgcc agtcgtcaat ggctccgttc cgcacgaggc
      901 gctgggcaag catggcgccg cgcgcgtact gctcaagccg gcgggcgccg gtaccggcgt
      961 tatcgcaggt ggaccggtcc gcgccgtgct cgaagccgcc ggcattacgg acatcctgac
     1021 caagagcctg ggctcgaaca atccgatcaa cgtggcgcgc gccacgatgg aaggactcga
     1081 gcagctggtt acgccggagc gtgtagccca ggagcgcggt atcacagtgg aagagctcgg
     1141 cttgccgcgc cggagaacag cgcaggtgcg tcgtggcgtc taagaagaag gcggcgggca
     1201 cgccgcgtcg gttgaagatc acacagatcc gcagcgcgaa tggtcgtccg gaaacgcatc
     1261 gcaggacgtt gcgtgcgctc gggttgaagc accagcactc tgtgattcac gatgaaaacg
     1321 aagccatcaa aggcatgctg ttccaggttc gtcatctcgt cgaggtgagc gaagtggaag
     1381 gtgaggaaaa gtaaatgtcg gacttgcata acctccgtcg ccctgagggc gcgaaccggg
     1441 gaaagaagcg tctgggacgt ggacccggct cgggcaaggg caagacgtcg gggaaaggtc
     1501 acaagggtca gaaggcacgt accggccatc acggcattcc gtcgtggttc gaaggcggcc
     1561 agatgccttt gcagcggcgt gttccgaagc gtggcttcaa gaacccgttc cgcaccgttt
     1621 ttgaagtggt gaacgtgggc cagctcaaca agctgtccga cgacaccatt acgccggaaa
     1681 caatgcattc caatcgcctg gtcgacatcg gtaagggaaa tccggtgaag gtgctgggcg
     1741 atggtgaggt cacgcgcaaa ttcacggtgc gtgcgcacgc gatcagcgac agtgcccgcg
     1801 ccaaaattga agcggctggc ggcacggtcg agctgctgga ggcctaaacc atggccaatc
     1861 cgatcccgaa tctgttcaag attccagagc tgaaagagaa aatcctcttt acgctcctga
     1921 cgttgctcat ctaccgggtc ggtgcacaca tcacggcgcc gggcatcgac gtcggcgccc
     1981 tgcgcgagca attcggtcag ttgcagagct cgctgttcgg cgtctacgac atgttcgtgg
     2041 gcggtgcgct ctcccgcgcc accatcttcg gcctcggcat catgccctat atctcggcga
     2101 gcatcatgtt ccagctgctc gccgcggtgg tgccggccat cgagaagatg cagaaagaag
     2161 gcgaggacgg tcgtaagaag ctgacgcagt ggacgcgcta taccacggtc gggctggcgc
     2221 tgctgcaggg ttacggctac gccgtgttcg tgcagtcgat cccgggtgca gtgcgcaatc
     2281 cgggcttctt cttcctgctg tcaaccgcgc tcattctgac catcggcgcc gtgttcgtga
     2341 tgtggctggg tgagcagatc accgaacgcg gcatcggcaa cggtatgtcg ctgctaatct
     2401 tcttctcgat catcgagagc ttccccacgc aggtcgccaa cacgtgggag caggtggcga
     2461 cggatgtcat atctccgttc gcggtcattc tcatggtcat catcatggtc gcggtcgtgg
     2521 cgggtgtagt ggcaatgacc atggcggcgc gcaaaatacc ggtgcagata ccgcgcaaag
     2581 tcatggggcg cgggcgggtg cgcgaaggcc agaagagttt cattccgctg cgcatcaact
     2641 cgtcgggcgt
//
LOCUS       DASURG010000587         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1121082, whole genome shotgun sequence.
ACCESSION   DASURG010000587 DASURG010000000
VERSION     DASURG010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121082"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFY80_10625"
     CDS             <1..797
                     /locus_tag="VFY80_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6008409.1"
                     /translation="KGEILGCFGLTEPDHGSDPGGMKARARRVDSGYIVSGTKLWITH
                     APIADIMVIWAKDDGGVVLGFILERGMKGLATRKIEGKFSVRISPTGEVSMDDVFVPD
                     ENVLPNVSGLKGPFGCLNNARFGICWGVFGAAEACWHTARQYVLDRKQFGRPLAANQL
                     IQKKLADMQTGIALGLLACLHVSRLREQGKATPEMVSLLKRHSAGTALDIARVARDML
                     GGNGISDEYPVIRHMCNLETVNTLEGTHDIHALVLGRAQTGISAFS"
     gene            complement(794..1450)
                     /locus_tag="VFY80_10630"
     CDS             complement(794..1450)
                     /locus_tag="VFY80_10630"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX6008410.1"
                     /translation="MLRKSARRISRIFLRGLVVILPIVITLALVYWLVVSTEALLGGI
                     VKLVLPDKYYTSGLGVLLGLILIFVAGLTINVWVTRMLIGWAEALMERIPIVKSIYGA
                     VRDLAEFLSGARSGQRFHKVVVVTIGENMRLMGFVTREDFSDQPAHIGATSDTIAVYL
                     PMSYQIGGFTVFLPRSSVQPIDMTIEDAMRYTLTAGMSGPKAENSAAPTGLAGKAASL
                     "
     gene            1715..2989
                     /locus_tag="VFY80_10635"
     CDS             1715..2989
                     /locus_tag="VFY80_10635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6008411.1"
                     /translation="MKPLNTRGRIAVLVMLAAVPALLLSLYLGVAQRWDSQSHAHSAL
                     KLRAQAAAAQLAGAAESGGLTVEKIDDAAAKFLPHDGTLAVLDQAGRVFARHPTTDRT
                     LSGTVLESINGKSASMFEAIDDKGISHIYALENIRTAPDGRGTLNVLLGVPRSVLYAE
                     AGRRTFGALAGFIIAAGLLLFAAWYGPEIAFLRRFRALLAVTNHIRAGDLTARTRFPQ
                     SDEELCHLGHALDDMAQALQKRDADLKHALRELSEQVITDPLTRLNNRRYLWDFLGRD
                     LIRARRAVLPVAAILFDIDHFKRFNDTWGHEAGDLVLKSVADVIRQNVRGSDIACRYG
                     GEEFIVVLPEATRNVAVERADQIRRDIERMELMLGERKLDRVTASFGVALYPTHADDA
                     EALVRVADDALYQAKEAGRNRVHVFETAPSLL"
     gene            complement(3002..3298)
                     /locus_tag="VFY80_10640"
     CDS             complement(3002..3298)
                     /locus_tag="VFY80_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008412.1"
                     /translation="MQDSMWRIGILLIGISGGAAVAADPASVSIQDLKPECKERHAAV
                     PPEHCLIDDRTSRLDAVRRAGSGDVVIIQSLPPPAAGRERPAASPTENVRPPVR"
     gene            3390..>4030
                     /locus_tag="VFY80_10645"
     CDS             3390..>4030
                     /locus_tag="VFY80_10645"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6008413.1"
                     /translation="MNRRIRLALAYVAIALTLAISPSAVGIELVNPYPAVGPREISGS
                     TPANRVLRTMQHHAVPPVTDILSQALQQVLSHALDIPVSVVRRPRAGGLDALRHVVEA
                     ARDSRTLLLGGSDTVVVQSLVKLDTRYDAVRDLIPIALVARMPLVLIAAADVSSVVHF
                     LDRSRNAGRSHLGSSGDLSTSQLAGALFKRTTQTPQVQVAFNGAHSAIRAVVA"
BASE COUNT          784 a         1244 c         1236 g          766 t
ORIGIN      
        1 cgaaagggga aatcctcggc tgttttggct tgacggagcc cgaccacggg tcggatccgg
       61 ggggcatgaa agcgcgcgca agacgcgtcg acagcggata tatcgtgagc ggcacgaaac
      121 tctggatcac gcacgcgccc attgccgaca tcatggtgat ctgggccaag gatgacgggg
      181 gtgtcgtgct tggtttcatc ctcgagcgcg gcatgaaagg gctcgccacg cggaaaatcg
      241 aaggcaagtt ttcggtgcgc atctctccca ccggcgaagt ctccatggac gacgtcttcg
      301 tgccagacga aaacgtgttg cccaatgttt cgggtctcaa agggcccttc ggctgcctca
      361 acaatgcgcg cttcggcatc tgctgggggg tgttcggcgc agccgaagca tgctggcata
      421 ccgcgcgcca gtacgtgctg gaccgcaagc agttcggacg accgctcgcg gcaaatcagc
      481 tgatccagaa gaagctcgca gacatgcaga ccgggatcgc gctgggcctc ctcgcgtgcc
      541 tgcacgtgag ccgcctgcgg gagcagggca aggccacacc ggaaatggtg tcactgctga
      601 agcgccatag cgccggcact gcgctcgata ttgcgcgggt ggcgcgagat atgctcggcg
      661 gaaacggcat atcggatgaa tatcccgtca tccggcatat gtgcaacctc gaaacggtca
      721 atacgctcga aggcacacac gacatccatg cgctcgtcct cggccgtgcg cagaccggaa
      781 tctcggcctt cagctagagg gatgccgctt ttcccgcgag accggtcggc gccgcgctgt
      841 tttccgcttt cggccccgac atgcctgctg tcagggtata acgcatcgcg tcctcgatcg
      901 tcatgtcgat cggctgtaca ctcgatcgcg gcagaaagac cgtaaagcct ccgatctggt
      961 agctcatcgg cagatagacg gctatggtgt cggacgttgc accgatatgg gccggctgat
     1021 cgctgaaatc ctcgcgcgtc acgaatccca tgaggcgcat gttttcgcct atggtcacca
     1081 cgaccacctt atggaagcgc tgcccggatc gcgcgccgga gagaaattca gcgagatccc
     1141 gaacggcgcc atatatgctc ttgacgatcg ggatgcgctc catcaaagcc tccgcccagc
     1201 cgatcagcat gcgcgtcacc cagacattga tcgtcagtcc ggcgacgaag atcaatatca
     1261 ggccgagcag cactccgagc ccgctggtgt agtacttgtc cggaaggacg agcttgacga
     1321 tgccgcccag caacgcctcg gtgctgacga ccagccagta aaccagcgcg agcgtgatga
     1381 cgatgggaag gatgacgacg agcccgcgca gaaatatcct ggatatgcgc ctcgccgact
     1441 ttcggagcat ggtgttcaag gtccgcctcc acttcaaaca ggatctgcgc gtagttcgac
     1501 acgtccgctc atgcttggag catggctccc accgaaagtg cctgcggctg agcatggcgg
     1561 ccgaggcatg cgattcaggg ccgaggatgc acttccacac tctgcgcttg taggatagca
     1621 ttccgagaca accgcgatgc cgtatgatcg caggcgaagc atcacggcgt cgaagaggct
     1681 ggacaattag aagagaaaga gagtaccgcc catcgtgaag ccccttaata cgcgcggccg
     1741 cattgcagtc cttgtgatgc tcgccgcggt tcctgcgctg ctgctttcgt tgtatctggg
     1801 tgtcgctcag cgctgggatt cacagtctca cgcccatagc gcccttaagc tcagggcgca
     1861 ggcggcggct gcacagctcg cgggcgctgc ggaatccggc ggcctcaccg ttgagaagat
     1921 agacgatgcc gcagcaaagt tcttgcccca tgacggcacg ctcgccgtac tcgaccaggc
     1981 gggccgcgtt ttcgcacgcc atccgaccac ggataggacg ctttcaggaa ccgtgctcga
     2041 gtcgataaac ggcaaaagcg cttccatgtt cgaggcgatc gatgataaag gcatatcgca
     2101 tatctacgcg ctcgagaaca tccggaccgc gccggatggt cgcggtacgt tgaacgtgct
     2161 gctgggcgtc ccacgctcgg tgctttatgc cgaagctgga agacgaacgt tcggggccct
     2221 ggcggggttc atcattgccg caggtcttct gctgttcgcc gcctggtacg gcccggagat
     2281 cgccttcctc agaaggttcc gtgcgttact ggcggtaacc aatcacatcc gagcgggcga
     2341 tctgaccgcg cggacccggt ttcctcagag cgatgaggag ctgtgccacc tcggacacgc
     2401 actggacgac atggctcagg ccttgcaaaa gcgcgacgcc gacctcaaac acgcactgcg
     2461 agagctgagc gagcaggtga ttaccgatcc gctgacccgg ctcaacaaca ggcgctatct
     2521 gtgggacttt ctcggccgtg acctcatacg cgcccgcagg gcggtattgc cggtggccgc
     2581 gatcctcttc gatatcgacc atttcaagcg cttcaacgac acatgggggc acgaggccgg
     2641 ggatctcgtc ctcaaatccg tagccgatgt cattcggcag aatgtaaggg gctcggacat
     2701 cgcgtgtcgt tacggcgggg aggagttcat cgtcgtcctg ccggaggcga cacggaatgt
     2761 ggctgttgag cgtgccgacc agatcaggcg ggatatcgag cgaatggagc tgatgctcgg
     2821 cgaacgcaaa ctcgaccgcg tcaccgcttc gttcggggtt gctctgtatc cgacgcacgc
     2881 ggacgacgcg gaagcgctcg tgcgcgttgc ggacgatgcg ctctaccagg cgaaagaggc
     2941 cggccgcaat cgggtacacg ttttcgagac ggcaccctcg ctgctctgaa aactgcaagc
     3001 ctcagcgcac cggcggcctg acgttctccg tgggcgacgc agccggacgc tctctgccgg
     3061 cagcgggagg cggaagtgac tggatgatca ccacatcgcc cgaacctgct cgccggacgg
     3121 catccagccg tgacgtacgg tcgtcgatca agcaatgttc cggcggcacg gcggcgtgtc
     3181 gttccttgca ttccggcttg aggtcctgaa tcgaaacgct cgcggggtct gccgcgactg
     3241 cggccccacc cgaaatgccg atcagcagta tgccgatccg ccacatggag tcctgcatgg
     3301 gctcgtcctc tgctccgagc ggagcacaag tatcttgact gttcgactac aggagtaccg
     3361 aacggttccg gctatacaat tccgtcacga tgaaccgccg cattagactt gcgctggcgt
     3421 atgtcgccat tgccctgacg ctcgctattt cgcccagtgc tgtgggaatc gagcttgtga
     3481 atccgtatcc cgctgtcggc cctcgtgaaa tcagcggcag cacgccggca aaccgggtgc
     3541 tgcgcacaat gcagcatcac gcagtgccgc ctgtcaccga catcctgtcc caagcccttc
     3601 agcaggtact ctcgcatgcg ttggacattc cagtatccgt ggtgcgtcgt ccacgtgctg
     3661 gcggactgga cgccctgcgg catgtggtgg aagcggctcg cgacagccgc accctgctgc
     3721 tcggaggaag tgatacggta gtcgtccaga gccttgtcaa gctcgacacg cgatatgatg
     3781 ccgttcggga tctgataccg attgcacttg tcgcgcgcat gccgcttgtg ctgatagctg
     3841 cggccgacgt ttccagcgtt gttcattttc tcgatcggtc acgcaacgcg gggcgttctc
     3901 acctcggatc ttcgggcgat ctcagcacct cccaactcgc cggtgcgctg ttcaagcgaa
     3961 cgacacaaac gccccaggtg caagtagcgt tcaatggcgc gcactccgcg atacgcgctg
     4021 tggtcgcgga
//
LOCUS       DASURG010000588         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1019450, whole genome shotgun sequence.
ACCESSION   DASURG010000588 DASURG010000000
VERSION     DASURG010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019450"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFY80_10650"
     CDS             complement(<1..582)
                     /locus_tag="VFY80_10650"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6008414.1"
                     /translation="MITRTLSQFVHDLSFDALPGEVRARVPALALDLTGIMVRARHEA
                     ESTPAMIAAARRLGLTPGACTVVGDASGYTPAGAAVVNGTLAHSLDFDDTHAPGSLHP
                     SAPIVPAAFAAAEMTGADGRAVLAAIVAGYEVQIRLSLALDPAAHYDRGFHPTATCGA
                     FGATAAAGRLLGLDAAGIASAFGIVLSMAAGSMQ"
     gene            complement(715..1848)
                     /locus_tag="VFY80_10655"
     CDS             complement(715..1848)
                     /locus_tag="VFY80_10655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6008415.1"
                     /translation="MAIPLTLALTFFNFVGANAARVVLTLYALELGAPASSVGVLGGL
                     LFLFPLLLSWPIGALADRTGARGILLFASCCGAASLVLPFFVRAVPAFYAAAALNGLA
                     LAFFHVTLQNLVGTLSHPEARARNFSNFSLTGAVTNFAGPLIAGFSIDHFGHAAACLV
                     VASQSVVAAVLVLAWGRLYPRGKKEAPIDSGTARALLNREVWYMLFVSGLVQLGTDLF
                     QFYLPIYGHSIGLSASAIGGVLATLAVAAFVVRLFLAWLVKRVAGEKLLMWVFLMGAI
                     GFALVPFSDSALVLGAIAFLFGLGMGIGIPLTVILMFSRSAEGRSGQTLGIRLTANNF
                     VRVTGPVVFGAIGTALGLAAVFWIVAAVMAAGALVSRRGTHVC"
     gene            complement(1855..2619)
                     /locus_tag="VFY80_10660"
     CDS             complement(1855..2619)
                     /locus_tag="VFY80_10660"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6008416.1"
                     /translation="MAAGRLAGSVAIVTAAGTGIGEAVARTFAREGASVVLVDIDEAA
                     VKRVADEIGASGGRAQAMRADMTRSQSVNDMTGEVVKRFGTVDILVNAAGGFHRLAPI
                     TGISDEEWDRVIDLNLKSVFYGSRAVASIMMEKRKGRIVNIASGAGIAPNPFAPSYLP
                     YGSAKAGVILFTKTLARDLGEYGITVNAVSPGTTRTPRVVKVRDAASLERIAAQNPMK
                     TLVEPIDSAEAVLFLASAEARYITGVNLNVNAGNLM"
     gene            2718..>3365
                     /locus_tag="VFY80_10665"
     CDS             2718..>3365
                     /locus_tag="VFY80_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008417.1"
                     /translation="MPETDLSSQYGAEAVAAGRSLQPRTFERRLAQRDSLDPHYTKLW
                     VDFAIEGLGRRPALDARTRLLVLIGQYTMARSEGALEDAIHAALEAKVAPREILEIIL
                     QCTVYGGHTVVEPAIECFHRIARDEGLLDALRASALPLDGNDRKRDYEVERATWHPDD
                     VRDPRCEPLMKKHGWLAVGRGLTLRPRHHLNILAWLDAIDSEFAGLWVKFCYGGMY"
BASE COUNT          634 a         1134 c         1104 g          493 t
ORIGIN      
        1 ctgcatggac cccgcggcca tgctgagcac gatgccaaag gcgctcgcga tgcctgccgc
       61 atcgaggccc agaagacgtc cagcagcggc agtggcaccg aatgcgccgc aagtcgccgt
      121 tggatggaac ccgcggtcat agtgggccgc cggatcgagc gcgaggctca aacgaatctg
      181 cacttcgtat cccgccacga tcgccgcgag gacggcacgg ccgtccgccc ctgtcatctc
      241 cgcggccgca aaagccgccg gtacgatcgg tgcactggga tgcagcgacc cgggcgcatg
      301 cgtgtcgtcg aaatcgaggg aatgcgcaag cgtgccgttc accaccgctg cccccgcggg
      361 cgtatacccg ctcgcatcgc cgacgacggt gcacgcgccg ggagtgaggc ccagacgccg
      421 cgcggcggca atcatggcag gcgtcgattc cgcctcgtgg cgcgcacgca ccatgatgcc
      481 ggtgagatcg agcgccagcg cgggcacgcg cgcgcgcact tccccgggca atgcgtcgaa
      541 gctgagatcg tgcacgaatt gtgaaagagt gcgggtgatc atcgatgtct cctggtcccg
      601 cgactcggaa tgcgcgcgag gcggacggcg tgctgcgtct ggaaattctg cacgacgcgc
      661 tccccggccg tcaacgcgcg cgggtgccgc ggcgtgaaac gagggcgccg agcgtcaaca
      721 cacgtgggtg ccgcggcgtg aaacgagggc gccggccgcc atgacggccg cgacgatcca
      781 gaacaccgcg gcgagtccga gcgccgttcc gatggcgccg aacacgactg gcccggtcac
      841 gcgcacgaag ttgttcgcgg tgagcctgat cccaagcgtc tggcccgagc ggccttccgc
      901 agagcggctg aacatcagga tcacggtcag cggaatgccg atgcccatgc cgaggccgaa
      961 gaggaaggcg attgcgccca ggaccaaagc gctgtcggaa aaaggaacga gcgcgaagcc
     1021 gatcgcaccc atgagaaaca cccacatgag gagcttctcg cccgcaacgc gcttcacgag
     1081 ccacgctagg aagagccgca cgacgaacgc cgcgaccgcc agtgtggcga ggacgccgcc
     1141 gatcgccgag gcggaaagcc cgatggaatg gccgtagatc ggcagataga actggaagag
     1201 atcggtgccg agctgcacga gtccgctcac gaagagcatg taccagacct cgcggttgag
     1261 cagcgcacgt gccgttcccg aatcgattgg cgcctctttc ttgccgcgcg ggtagagccg
     1321 gccccaggcg agcacgagca cggccgcaac caccgattgc gatgcgacca cgagacaggc
     1381 cgcggcgtgc ccgaaatgat cgatcgaaaa gccggcgatg agcggcccgg cgaagttcgt
     1441 gaccgcgccc gtcaggctga agttgctgaa attgcgtgcc cgtgcttcgg gatggctcag
     1501 cgtcccgacc aggttctgca gcgtcacgtg gaaaaaggcg agcgcgagcc cgttcagcgc
     1561 cgctgcggcg tagaacgccg ggaccgcgcg cacgaagaac ggcagcacga gcgaggcggc
     1621 gccgcagcag gaggcgaaaa ggagaatccc gcgtgcaccg gtgcggtcgg cgagcgcgcc
     1681 gatcggccac gaaagcagca gcggaaagag aaagagaagc ccgcccagca cgccgacgct
     1741 ggatgcaggt gcaccgagct ccagcgcata gagcgtcagc acgacgcgcg cggcattcgc
     1801 gccgacgaag ttgaagaacg tcagcgcgag cgttaaaggg atcgccacgc ggcgttacat
     1861 caggtttccg gcattgacgt tcagattgac gccagtgata tagcgcgcct ccgcggaagc
     1921 gaggaagagc acggcttccg cgctgtcgat cggctcgacc agcgtcttca tcgggttctg
     1981 cgccgcgatg cgctcgaggc tcgccgcatc gcgcaccttc accacgcgcg gcgtgcgggt
     2041 cgtgccaggc gatacggcgt tcaccgtgat gccgtactcg cccagatcgc gcgcaagcgt
     2101 cttggtgaag agaatgacgc ccgccttggc cgacccgtac ggcagatacg acggggcgaa
     2161 cggattgggt gcgatgccgg cgcccgaggc gatattgacg atgcgccctt ttcttttctc
     2221 catcatgatc gacgccacag cgcgcgaacc gtagaacacg cttttcaggt tgagatcgat
     2281 cacgcggtcc cattcctcat cgctgatgcc cgtgatcggc gcgaggcggt gaaaaccgcc
     2341 cgcagcgttg acgagtatgt ccaccgtccc gaaccgcttt accacctcgc ccgtcatgtc
     2401 gttcaccgat tgtgagcgcg tcatgtcggc gcgcatggcc tgcgcgcgcc caccgctcgc
     2461 gccgatctca tcggcgacgc gcttcaccgc cgcttcatcg atatcgacga gcacgacgct
     2521 cgcgccttcg cgcgcgaacg tacgcgcgac cgcctcaccg atgcccgtgc ctgcggcggt
     2581 gacgatcgcg accgatcctg cgagtcttcc tgctgccatc gctcaaatcc tccctggaat
     2641 agcccctata ataccaagcg cgcgagcgcc cattcgcgtg ttcgttccat ccctttcgac
     2701 acccagccgg agtagccatg cccgagaccg atctttcctc ccagtacggc gccgaggccg
     2761 ttgcagccgg tcgcagcctc cagccgcgca cctttgaacg ccggctcgcg cagcgcgaca
     2821 gcctcgaccc gcactacacg aaactgtggg tcgatttcgc gatcgaggga ttgggcaggc
     2881 ggccagcgct cgatgcgcgt acccggctcc tcgtgctgat cggtcagtac accatggcgc
     2941 gaagcgaggg cgcgctggag gacgccatcc atgccgcgct cgaggcgaag gtggcgccgc
     3001 gcgaaatcct cgaaatcatc cttcaatgca cggtttacgg cgggcatacg gtcgtcgagc
     3061 ccgcgatcga atgcttccat cgcatcgcgc gcgacgaggg tttgctcgac gcgctccgcg
     3121 cgagcgcgct gccgctcgac ggcaacgacc gcaagcgcga ctacgaggtg gagcgggcga
     3181 cctggcaccc cgacgatgtc agggacccgc gctgcgaacc gctcatgaag aagcacggct
     3241 ggctcgccgt cgggcgaggc cttacgctgc ggccccggca tcacctcaac atcctcgcct
     3301 ggctcgacgc gatcgacagc gaattcgccg gtctgtgggt caagttctgt tacggcggga
     3361 tgtac
//
LOCUS       DASURG010000589         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_508536, whole genome shotgun sequence.
ACCESSION   DASURG010000589 DASURG010000000
VERSION     DASURG010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508536"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1538
                     /locus_tag="VFY80_10670"
     CDS             150..1538
                     /locus_tag="VFY80_10670"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6008418.1"
                     /translation="MPLASGERRGQVRLYGSAELLAAMDDKVLEQITNVARLPGLAGP
                     AMTMPDAHWGYGFPIGGVAAFDPELGGIISAGGVGFDISCGIRCLRTNLDLGDIAPRM
                     RVLADRLYRDIPAGVGEEGQLSLTPDELDEVLVGGARWAVENGYGVADELEYIEERGT
                     VEGAVPTNVSNLAKKRQRGEMGTLGSGNHYLEVQVVDRIYDADIAAAFGLHANQIIVS
                     IHCGSRGLGHQIGTDYLVSLAKAAGRLGIELPDRELASAPIQSPEGQQYVGAMNAAIN
                     CALANRQILAHLTRHTFARDFPHAALETLFDVSHNTCKAERHIVHGKSRLLYVHRKGA
                     TRAFGPGHAAVPVRYRAHGQPVIIGGSMGTGSYILCGMQEGEGRALSSASHGAGRAMS
                     RHQALKQWRGRELIDELAGRGILVRTRSMRGAAEEAPGAYKDVDLVAESTEHAGLARR
                     VAFLRPKICVKG"
     gene            1663..2112
                     /locus_tag="VFY80_10675"
     CDS             1663..2112
                     /locus_tag="VFY80_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6008419.1"
                     /translation="MTIGDICNREVVYAPRDATVYDGAQLMRHRHVGTLVIVDEFAGK
                     RVPVGIVTDRDIVVEVNAVDLDPKTITVGDIMDPSLVTVPEAANLPEAVELMRAKGVR
                     RLPVVGREGELVGIVSIDDVLEVLSDELSDVARIIAREQAHEARARR"
     gene            2116..2586
                     /locus_tag="VFY80_10680"
     CDS             2116..2586
                     /locus_tag="VFY80_10680"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX6008420.1"
                     /translation="MKQTCLIVADAGRARLFVVAPEEGPRRRPRLLEQAALADGEVKT
                     RSRTGSGAVSSERNTNRKAGPMHPIAAQRERHRLEHERRFGAEIVRHAVELTADWSEG
                     VMVVIAEPRLLGLMRERLREALPPRIRLEALAKDYSGLSKSALERILISQRLIE"
BASE COUNT          486 a          839 c          917 g          437 t
ORIGIN      
        1 taccatgctc tgcgtcaggc aggtgaacgg ctcctgggaa gcacgctgcg tggtggacgt
       61 gtagcaggct tccgcgtact cgacgcgaat ctcgtcccag cgccatggag ctctcccgtc
      121 taactcagat cgacccgtgg tgctggaccg tgccgcttgc gtcgggtgag cgacgcgggc
      181 aagtgcgcct ctatggcagc gccgaattgc ttgcggcgat ggacgacaag gtgctcgagc
      241 aaatcacgaa tgtcgcgcgt ttgcccgggc tcgcggggcc cgcgatgacc atgcccgatg
      301 cccactgggg ctacgggttt ccgatcggcg gcgtcgcggc gttcgatccc gagctggggg
      361 ggatcatctc cgcgggcggt gtcggattcg atatctcatg cggcatccgg tgcctgcgta
      421 ccaatctcga ccttggcgac atcgccccgc gcatgcgcgt gctcgcggac cggctctatc
      481 gcgacatacc ggcgggcgtg ggagaggaag ggcagctcag cctgacgccg gacgagctcg
      541 atgaagtgct cgtcggcggc gcgcggtggg cggtagagaa cggctacgga gtcgccgacg
      601 agctggaata cattgaggaa cgcggcactg tggaaggggc ggtgccgaca aacgtctcga
      661 acctcgcgaa aaaacgacag cgcggcgaga tggggacgct cggctccggc aaccactatc
      721 tcgaggtgca ggtggtggac cgcatctacg acgcggacat tgcggcggca ttcggcctgc
      781 acgcgaacca gatcatcgta tcgatccatt gtggatcgcg cgggctgggc catcagatcg
      841 ggaccgatta cctcgtaagt ctcgccaaag cggcaggtcg cctcggaatc gagctgccag
      901 accgggagct cgcctccgcg ccgattcagt cccctgaagg gcagcagtac gtcggcgcca
      961 tgaatgcagc gatcaattgt gcgctcgcca atcgccagat tctcgcgcac ctcacgcgcc
     1021 acacattcgc gcgggacttc ccgcacgccg cgctcgagac gctcttcgat gtgtcgcaca
     1081 acacgtgcaa agccgaacgg cacatcgtgc acggcaagtc gcgcctcctg tacgtgcatc
     1141 ggaagggcgc aacacgcgca ttcggcccgg gccatgccgc cgttccggtg cgctatcgcg
     1201 cgcatggcca gccggtcatc atcggcggca gcatgggcac cggctcgtac atcctgtgcg
     1261 ggatgcagga gggcgaaggg cgcgcgctga gttccgccag ccacggggcg ggccgggcga
     1321 tgagccgcca ccaggcgctc aagcaatggc gtgggcggga actgatcgac gagcttgcgg
     1381 ggcgcggcat cctcgttcgc acgcgatcga tgcgcggcgc ggctgaagaa gccccgggcg
     1441 catacaagga cgtcgatctc gttgccgaat ccaccgagca cgcgggcctg gcgcggcgcg
     1501 tggcgtttct ccggccgaag atctgcgtca agggctagcc cgttacgccc ggcgcgcgag
     1561 gtgcggtctg cccttgatct ggatcaatgg gggtctcgcg cgcgtaatta acatgatttt
     1621 tgaaccgccc tgaagcgctg tgttccagca aggagaattg caatgacgat cggcgacatc
     1681 tgcaaccgcg aggtggtgta tgccccgcgc gacgcgaccg tttacgacgg cgcacaactc
     1741 atgcgccatc gccatgtcgg gacgctcgtc atcgtggacg aattcgcggg caagcgcgtt
     1801 ccggtcggca tcgtgaccga ccgcgacatc gtcgtggaag tgaatgcggt cgatctcgat
     1861 ccgaagacca tcaccgtggg cgacatcatg gacccgtcgc tcgtcaccgt ccccgaggcc
     1921 gcgaatctgc cggaagcggt cgagctgatg cgggccaagg gcgtgcgcag gctccccgtc
     1981 gtcggacggg aaggcgagct tgtcggcatc gtttctatcg acgacgtgct ggaagtgctc
     2041 tctgacgagc tttccgacgt ggcacgcatc atcgcgcgcg agcaggcgca cgaagcccgg
     2101 gcacgacgct gaatcttgaa gcagacgtgc cttatcgttg cggacgcagg gcgggcgcgc
     2161 ctttttgtcg tcgcgccgga agagggaccg cgccgcaggc cgcgtctgct cgagcaggcg
     2221 gccctggctg atggcgaagt gaagacgcgc agcagaacag gaagcggtgc ggtgagcagc
     2281 gagcgcaaca cgaatcgcaa ggcggggccc atgcatccca tcgccgcgca gcgcgagcgt
     2341 catcggctcg agcacgagcg tcggttcggc gcggagatcg tgcggcatgc cgtggagctg
     2401 acggcggact ggagcgaagg tgtcatggtg gtcattgccg aaccgcgcct gctcggactt
     2461 atgcgcgaaa gattgcgcga ggcgctgccg ccccgcatcc ggctggaagc gctggccaag
     2521 gattacagcg gactcagcaa gtccgcactc gagcgcatcc tgatctcaca acgtctcatt
     2581 gaataagtcg atgagacgcg ccggctgcac gtaacgtgat accgcgagtc atccggaaac
     2641 acacgcgggc aggtcgaaag cccaacctgt cggactacg
//
LOCUS       DASURG010000590         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_814790, whole genome shotgun sequence.
ACCESSION   DASURG010000590 DASURG010000000
VERSION     DASURG010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814790"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="ilvN"
                     /locus_tag="VFY80_10685"
     CDS             complement(<1..341)
                     /gene="ilvN"
                     /locus_tag="VFY80_10685"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX6008421.1"
                     /translation="MRHIISVLLENEAGALSRVSGLFSARGYNIESLTVAPTEDETLS
                     RMTVVTSGSDEVIEQITKQLNKLIDVVKVVDLSEGDHIERELMLVKVRAIGKDREEMK
                     RMADIFRGRII"
     gene            complement(338..2035)
                     /locus_tag="VFY80_10690"
     CDS             complement(338..2035)
                     /locus_tag="VFY80_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEX6008422.1"
                     /translation="MELTGAEIVVRCLQEEGVEYVFGYPGGAVLFIYDELFKQNNVKH
                     ILVRHEQGAVHAADGYARSTEKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIVTGQV
                     PTHAIGQDAFQECDTVGITRPCVKHNFLVKDVRDIAATMKKAFYIASTGRPGPVVVDI
                     PKDVTVQKAAFAYPETTVMRSYNPVVKGHSGQIKKAIQVLMGAQRPMIYTGGGVILGN
                     ASAELAQLVRLLGFPCTNTLMGLGGYPATDRQFVGMLGMHGTYEANMAMQHCDVLLAI
                     GARFDDRVIGNPKHFFEESRTIIHIDIDPSSISKRVKVDVPIVGNVREVLRELIRQLE
                     ASHARPDQEALKTWWTQIELWRARDCLKYDRASPLIKPQYVIEKLYEITGGDAFVTSD
                     VGQHQMWAAQFYKFDRPRRWINSGGLGTMGFGLPAAMGAQLAHPEAEVACVTGEASIQ
                     MCIQELSTCKQYRLPIKIVNLNNKYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAES
                     YGHVGMRVEKPEDVEPVLREAFSRKDDLVFMDFVTDQTENVFPMVPGGKGLSEMILV"
BASE COUNT          387 a          708 c          695 g          427 t
ORIGIN      
        1 tcgatgatgc gcccgcggaa gatgtccgcc atgcgcttca tctcctcgcg gtccttgccg
       61 atcgcgcgca ctttcaccag catgagttcg cgctcgatgt ggtcgccttc ggacagatcg
      121 accaccttta cgacgtcgat cagcttgttg agctgcttcg tgatctgctc gatcacttca
      181 tcggaaccgc tggtgacgac cgtcatgcgc gaaagggtct cgtcctcggt gggcgcgacg
      241 gtgagcgatt cgatgttgta gccgcgcgcg gagaaaagac ccgacacgcg cgagagcgcg
      301 cccgcctcgt tttcgagcag cacggaaatg atgtgcctca tacgaggatc atctcggaga
      361 ggcccttgcc ccccggcacc atcgggaaca cgttctcggt ctggtcggtc acgaaatcca
      421 tgaagaccag atcgtccttg cgcgagaagg cttcgcgcag caccggctcc acgtcctcgg
      481 gcttctccac ccgcatgccg acgtgaccgt aagattctgc gagcttgacg aaatcgggca
      541 gcgcgtccat gtacgactcg gcgtagcggt tgccgtggaa gaattcctgc cactgccgca
      601 ccatgcccat gtacttgttg ttgaggttca cgatcttgat cggcaggcgg tactgcttgc
      661 aggtcgagag ttcctggatg cacatctgga tgctcgcctc gcccgtgacg cacgcaacct
      721 ccgcctcggg atgcgcaagc tgcgcgccca tcgccgccgg cagcccgaac cccatggtgc
      781 ccagcccgcc cgaattgatc cagcgccgcg gccggtcgaa cttgtagaac tgcgcggccc
      841 acatctggtg ctggccgacg tcggaggtga cgaaggcgtc gcctccggta atctcgtaga
      901 gcttctcgat cacgtactgt ggcttgatca gcgggctcgc gcggtcgtac ttgaggcagt
      961 cgcgcgcgcg ccacaactcg atctgcgtcc accaggtctt cagcgcttcc tggtccggtc
     1021 gtgcatggga cgcttcgagc tggcggatca gttcgcgcag cacctcgcgc acgttgccga
     1081 cgatcggcac gtccaccttg acgcgcttgg agatcgacga gggatcgatg tcgatgtgga
     1141 tgatggtgcg cgactcctcg aagaagtgct tcgggttgcc gatcacgcgg tcgtcgaagc
     1201 gcgctccgat cgcgagcagc acgtcgcagt gctgcatcgc catgttggcc tcgtaggtgc
     1261 cgtgcatgcc gagcatgccg acgaactggc ggtcggtcgc gggataaccg cccagcccca
     1321 tcagcgtatt ggtgcagggg aacccgagca ggcgcacgag ctgcgccagc tctgcggagg
     1381 cgttgccgag gatcaccccg cccccggtgt agatcatcgg acgctgcgcg cccatcagca
     1441 cctggatcgc cttcttgatc tgccccgagt ggcccttgac gaccgggttg taggagcgca
     1501 tcaccgtcgt ctcgggatac gcgaacgcgg ctttctggac ggtcacgtcc ttcggaatgt
     1561 ccaccacgac cgggccgggg cgaccggtgg aggcgatgta gaaggccttt ttcatcgtcg
     1621 ccgcgatgtc ccgcacgtcc ttcaccagaa aattatgctt cacgcacggg cgcgtgatgc
     1681 ccacggtgtc gcattcctgg aacgcgtcct ggccgatggc gtgcgtgggc acctgaccgg
     1741 tgacgatgac gagcgggatc gaatccatgt aggcggtcgc gatgccggtc acggcattgg
     1801 tgacgccggg tccggaagtg acgagcgcca ccccgacctt ctcggtggag cgggcgtagc
     1861 cgtcggcggc gtggaccgcg ccctgctcat ggcgcaccag gatgtgcttg acgttgtttt
     1921 gcttgaacag ctcgtcgtag atgaacagca cggcgccgcc ggggtagccg aagacgtact
     1981 cgacaccctc ctcctgcagg catcgcacga cgatttcagc gccggtgagt tccataagcc
     2041 cgattcgcga ccaagtagcg gaaaacttag gacattaccg ggtggccgcg cgttggtcaa
     2101 gcaaaatatc cctgtaaaca gcatgataca cgccacgctg cgagcgcttt cccacgcggc
     2161 ttgcgccgct gaaacgtttt gctaagatgc cctgcgaccc ggtacgaaag cgggaac
//
LOCUS       DASURG010000591         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_713316, whole genome shotgun sequence.
ACCESSION   DASURG010000591 DASURG010000000
VERSION     DASURG010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713316"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..573)
                     /locus_tag="VFY80_10695"
     CDS             complement(298..573)
                     /locus_tag="VFY80_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX6008423.1"
                     /translation="MKTKDAVHNPIAINPETCVRCDLCDWICPGDIIYKEEDNRETLP
                     VIAYPDECWYCGLCESICPTDAITVVFPEQMVHNKTDVVSLLGRVVD"
     gene            complement(587..2302)
                     /locus_tag="VFY80_10700"
     CDS             complement(587..2302)
                     /locus_tag="VFY80_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6008424.1"
                     /translation="MAVQDGWTTLDTDVLVVGGGVAGSMAAIPALEAGLQVVICEKGK
                     VLDRCGSIGCGVDHYLTVMDSGPEWDTPDFLIKHVPELTDGIVDMAVAGRVIREMPRI
                     FRKLEALGVDFKDRKTGSYYRLRSFGLPGTYHINFDGTDFKKHIGERVRKGKATVLMR
                     TMVLQLLTHENRAYGALAFNFRTGEWYAIRAKAVVLAAGDVNRISKNASGLAFDSWHF
                     PYDTGDAHAMGYRSGAMLANMESVEATLTPKGFSCQGLNALVSLGAYFVNRHGERFMF
                     KYDKKGENARRAVIADAAINEYLLGNGPVYLDCRHLPQEMLDRMEYTLQVDRYTLPAF
                     YRQKGVNFREELVEISVSELSIRRSGVYFRGSGLAVDVNGETSVEALFAAGDCATVSG
                     GIAGAAVLGHIAGEGTVRRIAATPGTLPPVDMDAASSLRAEAQAVLARSDGMSWKALE
                     ERTRQTVTDYVGVRRNEKGLRLALEALKALARHEPTLRADDLHGLMRVHEAKNIRLNA
                     ELMATASLARNETRTGSSHCRLDYPQTDDVNWRKFVIVSRSEDGPAVRTLPTERPLAD
                     AFARA"
     gene            complement(2428..3336)
                     /locus_tag="VFY80_10705"
     CDS             complement(2428..3336)
                     /locus_tag="VFY80_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6008425.1"
                     /translation="MSRAIRTYTLSERVTGPDFGIRDERSVTRIGEAHRHEYFQIQLN
                     LAGATEHHIGATVRPFEPGSVSFVLPYRVHRVPHPPGSRFYVLSFNQRMLRPELEVDP
                     LDLEDVPLERAPELAPFRFQEFLDFRLDGADLAFAEAACVRMAQENAHRRFCSIELIR
                     GNLLLLIGTVCRRYERALLDLASAQAQRRSRRDALFRVVRYVRENLARRMSLADAAAA
                     ADLSPNYLAHLLKKETGKTFTDLVTERRMEKAQELLAHTTLRIAEVAEAVGFEDEAYF
                     ARRFRQCFKVAPREYRNRHYSGTDHR"
BASE COUNT          618 a         1164 c         1136 g          675 t
ORIGIN      
        1 tcgaagatcg cgatctcgtt gccctgcggc tcgtagtagg gcgcgacgtc gcctgcgacc
       61 ggcgcctgca cgatgtcgcg ttcgacgccg cgcaggcgat tcactgctgc tgttttcatt
      121 tccaccccga aatccagttt caccgcccag gctgccggaa aagaaacccg cgcgcatgag
      181 cgccgcgggt ttgtctgatg ttaatacagc gtgcccggcg cacaagttcc cgccgcaccg
      241 gcccgccaat gcattgtagc aaaccgccct aagcggctga aaatggagtg ggttcggtca
      301 gtcgaccacc ctgccgagca gggagaccac gtcggtcttg ttgtggacca tctgctccgg
      361 gaagacgacg gtgatggcgt cggtggggca gatcgactcg cagaggccgc agtaccagca
      421 ttcgtcggga taggcgatta ccggcagggt ctcgcgattg tcctcctcct tgtaaatgat
      481 gtcgccggga cagatccagt cgcacagatc acaacgcacg caggtttcgg ggttgatcgc
      541 gatggggttg tgcacggcat ccttggtctt cacggccaca ttcctttcag gcacgcgcaa
      601 aggcatccgc gagcggccgc tccgtgggca gcgtgcgcac ggccggtccg tcttcgctgc
      661 gggagacgat cacgaatttg cgccagttga cgtcgtccgt ctgcgggtag tcgagccggc
      721 agtgcgacga gccagtgcgc gtctcgttcc gcgcaagcga agcggtcgcc atcagctcgg
      781 cgttcagccg gatgttcttc gcctcgtgca cccgcatgag cccgtgcagg tcgtccgctc
      841 tcaaggtcgg ctcgtgccgc gccagcgcct tcagcgcttc aagagcgagc cgcaggccct
      901 tctcgttgcg acgaacgccg acgtaatcgg tcacggtctg ccgcgtgcgc tcttcgagcg
      961 ctttccaact catgccgtcg ctccgcgcga gcacggcctg cgcttcagcc cgcaggctgc
     1021 tcgcggcatc catgtccacc ggcggcaggg tgcccggcgt agcggcgatg cgccgcaccg
     1081 ttccttcgcc cgcgatatgg ccgagcacgg ccgcgccggc aatgccgccg ctcaccgtcg
     1141 cgcaatcgcc ggcggcgaag agcgcttcaa cggacgtctc cccgttcacg tccaccgcga
     1201 gcccgctacc gcggaaatag acgccgcttc tccggatcga aagctccgag acggaaatct
     1261 ccaccagctc ttcgcgaaaa ttgaccccct tctgcctgta gaacgcgggc agggtgtagc
     1321 ggtccacctg caacgtgtat tccatccggt cgagcatttc ctgtggcagg tgacggcaat
     1381 cgaggtacac cgggccgttg ccgaggaggt attcgttgat cgccgcatcc gcgatgacgg
     1441 cgcgccgggc gttctcgcct ttcttgtcgt acttgaacat gaagcgctcg ccgtggcggt
     1501 tcacgaaata ggcgcccagg ctcacgagcg cattgagacc ctggcatgag aatcctttgg
     1561 gcgtgagcgt ggcctcaacc gactccatgt tcgcgagcat tgcgcccgag cggtagccca
     1621 tcgcgtgcgc atccccggtg tcgtagggga aatgccagct gtcgaaggcg aggccggatg
     1681 cgttcttcga tatgcgattc acgtcgccgg ctgcaagcac caccgctttc gcgcggatcg
     1741 cataccactc gccagtgcgg aaattgaacg cgagcgcgcc gtatgcgcga ttctcgtgcg
     1801 tgagaagctg cagcaccatg gtgcgcatga gcacggtcgc cttgcccttg cgtacgcgtt
     1861 cgccgatgtg cttcttgaag tccgtgccgt cgaagttgat gtgataggtg ccgggtagcc
     1921 cgaacgagcg cagccggtaa tagctgccgg tcttcctgtc cttgaaatcc acgccgagcg
     1981 cttcgagctt gcggaagatg cgcggcattt cgcggatgac acggcctgcg acggccatgt
     2041 cgacgatgcc atcggtcagc tccggcacgt gcttgatcag aaaatccggc gtgtcccact
     2101 cggggccgga atccatcacc gtgaggtagt ggtccacccc gcagccgatg ctgccgcagc
     2161 ggtcgagcac cttgcccttc tcgcagatca cgacctgcag gccggcttcg agcgccggga
     2221 ttgcggccat gctgccggca acaccgccgc ccaccaccag cacgtccgta tcgagcgtcg
     2281 tccagccgtc ctgtaccgcc acgtcgagcc ccgccggttt atcaagaccc tgaaggatag
     2341 aagctttgcc cggcggcggc cagagacggt attggctttt ttctggactt ttgtgacctc
     2401 ggggacagac cctgcagtgg aaacacctca gcggtggtcg gtcccgctgt aatgtcggtt
     2461 gcgatactcg cgtggggcta ctttgaagca ctgccggaac cgccgcgcga agtaggcttc
     2521 gtcctcgaac ccgaccgcct cggcgacttc cgcgatacgc agcgtagtgt gcgcgagaag
     2581 ctcctgcgcc ttttccatgc gccgctcggt gacgagatcc gtgaaggtct tgcccgtctc
     2641 cttcttgagg agatgcgcga ggtagttcgg cgagaggtct gcagcagccg cggcatcggc
     2701 aagcgacatg cgacgcgcga ggttctcgcg tacatagcga acgacccgga agagcgcatc
     2761 gcgacgactg cggcgctgcg cctgggccga tgcgagatcg agcagcgcgc gctcgtagcg
     2821 gcggcatacg gtgccgatga gaagcagcag gttcccgcgg atcagttcga tcgagcagaa
     2881 gcgccgatgg gcgttctcct gcgccatgcg gacacacgcc gcctcggcga acgcaagatc
     2941 ggcgccatcg agtcggaagt cgagaaattc ctggaaacgg aacggagcga gctccggtgc
     3001 gcgttcgagc ggcacgtctt cgagatcgag cgggtccact tcgagttccg gccgcagcat
     3061 gcgctggttg aagctgagca cgtagaaccg cgatcccggc gggtgcggca cgcggtgcac
     3121 ccgatacggc agcacgaagc tcacgctccc cggctcgaac ggccgcaccg tcgccccgat
     3181 gtggtgctcg gtcgcgccgg cgagattgag ctggatctgg aaatactcgt gccggtgcgc
     3241 ttcgccgatg cgcgtcacgc tgcgctcgtc gcgaatgccg aagtcggggc cggtcacgcg
     3301 ctccgacaag gtgtacgtcc tgatcgcccg gctcatggcg cgccgactat atgcgcccac
     3361 ggtctacgcc gcaacaatcg cgaacgaaaa aacaaaaagg gcgaaccgca gttcgccctt
     3421 tgcataaaag cggggaactg tcccgttcac tgaatggctt tgaccgcttc cttgaggatg
     3481 ggcgtcaggc tcttgccttc cttgaagatg ttcgcgacga ctttctgctg ctgcgcgccg
     3541 tccatgtagc tcacgggacg gttgcccttc ttggtctctg cgatgaattc ctg
//
LOCUS       DASURG010000592         4626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_305966, whole genome shotgun sequence.
ACCESSION   DASURG010000592 DASURG010000000
VERSION     DASURG010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305966"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFY80_10710"
     CDS             complement(<1..772)
                     /locus_tag="VFY80_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6008426.1"
                     /translation="MGKLEGKVAFLTGAGAGIAKASALLFAREGAKVAIVEINPETGR
                     ATETEVRKAGGEALFIETDVTQDESVKRAIEGSVAKLGRLDVLMNCAGGSLQEDVPVH
                     EMDLAIWQRTIALNLLHPFLVCRHGIPHMMRAGGGSIINVTSHLGLMGSIKPAYAATK
                     GGIISFTKTLAAQYVDHHIRANAIAPGSVRTERQIRRYENREWVESEKTGAAARERMI
                     AQKLYPYSVSEPSDIAAIALFLASDDSRTLTGTTIAADG"
     gene            complement(785..1762)
                     /locus_tag="VFY80_10715"
     CDS             complement(785..1762)
                     /locus_tag="VFY80_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008427.1"
                     /translation="MRAITRKVASLGLVLSFAGGALPSLAQTPYPARPIRFLVGVVPG
                     GAADLLARAIGQRLGERLGTQVIVDNRPGANQTIAAHLTATATPDGHTILIVPSGHAI
                     SPSMYALKYDSVNDFSAIGLVAMVPNVLVVHPAIPARSVKDFIAYARTKPGELNMGSS
                     GTGSASHLAGELFQMMTQTRLTHVPFKGQGAAMVDLVAGRLQTGFPSVPASLPHVKTG
                     RMHALGVTPRNRSSAMPDVPTLEEAGVTGYEVAGWYGVLGPARIPKRIVGRLNEEIAL
                     MMRDPTMRETLTRAGADPLSSTPEEFRRIIADDIGKWAKVVKAAGVKMQ"
     gene            complement(1785..2759)
                     /locus_tag="VFY80_10720"
     CDS             complement(1785..2759)
                     /locus_tag="VFY80_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008428.1"
                     /translation="MRIGKCIVAVLFGAMGAAAGAQGWTPQKNVELVVPNPPGGSNDK
                     TARTVERILKANNLVPSTLTVVNKPGGGGSIAYTYVQQHAGDPHYLVVAGPALLTNHI
                     TGASKLRHSDLTPIAALFDDYTAFAVAAGTPLRSGKDLIDRLQKDPKSVTIGFSPLLG
                     SHNHIAAGLLMKTIGGNARDLKVVAYKGSADAIPNLMGGHIDLVTTAVGNVAGHVTNG
                     KLNIVAITATQRFTGALAHVPTWKEQGVDHVFGAWRAIFAPKGITPEQAAYWAGALRK
                     ATEAPEWKQDLERNYWVNVFTAGDAFAKELERDYDAMKAVLVDIGLAK"
     gene            complement(2781..3866)
                     /locus_tag="VFY80_10725"
     CDS             complement(2781..3866)
                     /locus_tag="VFY80_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008429.1"
                     /translation="MGEILGLGITHYPNLSAKRNMSWRMQQCLADPALDERLRSPQNW
                     HPTMREQWGGDEGQAHSDRHRRDLVERFRWARAELDAFKPDFVLMWGDDQYENFREDC
                     VPAFAILAYDRVSFQPWKNYRRGPNAWDEPEDQTFTVAGHRAGGKYLATALLRVGFDV
                     AYAYQPLHRPLGHAFSNSILYLDYDRRGFPYALVPFAINAYGRWLIAGRGAPMRPSEA
                     AEQLAEGESALDPPAPQPWRCFELGAAVARALKHSPWRVALIASSSWSHSFLVQKHSH
                     MFPDVEADRSYYEALKRGDWQYWRNTTLEEVEDRGHHELLNWFCLAGAMHELGRREPD
                     ESVFLESWITNSDKVFAVFRPHAARAA"
     gene            4076..>4626
                     /locus_tag="VFY80_10730"
     CDS             4076..>4626
                     /locus_tag="VFY80_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008430.1"
                     /translation="MANGLKPIRRVVTGNDERGRSKVVWDGPAPNSHEASMGSGRGHT
                     DLWVWNDTPAPLSGASDDGNLPYTFCGPQNGGHFRIVQARPRTADYSPAKDKEAVAPH
                     PPKLRPGGRGTWDRGGNNSYSSAMHKTETVDYGIVMAGERGLILDDCELTMKPGDIVI
                     QVGAWHQWSMPKGAMMAFDMIGAR"
BASE COUNT          811 a         1512 c         1477 g          826 t
ORIGIN      
        1 cgccatcggc ggcgatagtg gtgcccgtaa gtgtgcgcga atcgtcggat gcgagaaaaa
       61 gcgcaatcgc cgcaatatcg gacggctcgc tcaccgaata tggatagagc ttctgggcga
      121 tcatgcgctc gcgcgccgcc gcgcccgtct tctccgattc gacccattcc cggttctcgt
      181 agcgcctgat ctggcgctcg gtccgcaccg atcccggcgc gatggcgttg gcgcgaatgt
      241 ggtgatcgac atactgcgcc gcgagggtct tcgtgaacga gatgatgccg cccttggtcg
      301 cggcgtatgc gggcttgatc gagcccatga gcccgagatg ggacgtgacg ttgatgatcg
      361 agccgccgcc cgcgcgcatc atgtgcggaa tgccgtgacg gcagacgagg aacgggtgca
      421 ggagattcag cgcgatggtc cgctgccaga tcgctaggtc catctcgtgc accggcacgt
      481 cttcctgcag cgagccgccg gcgcagttca tgaggacatc gaggcgcccc aatttcgcga
      541 ccgacccctc gatcgcgcgt ttgacgctct cgtcctgcgt gacatcggtc tcgatgaaga
      601 gcgcttcgcc gcccgccttg cgcacttccg tctcagtggc gcggccggtt tccggattga
      661 tctccacgat cgccaccttg gccccctcgc gcgcgaaaag aagcgcgctc gccttcgcga
      721 tgccggcccc cgcaccggtg agaaaggcca ccttgccttc gagcttgccc attgtttgtc
      781 cttgctactg cattttcacg ccggccgcct tcaccacctt cgcccacttg ccgatgtcgt
      841 cggcgatgat tctgcggaat tcttccggcg tgctcgacag cgggtcggcg ccggcgcgcg
      901 tgagcgtctc gcgcatcgtc ggatcgcgca tcataagtgc gatctcttca ttcagccgcc
      961 cgacaatcct cttcgggatg cgggcgggtc cgaggacgcc gtaccagcct gcaacttcgt
     1021 atcccgtgac gccggcttcc tccagcgtcg gcacatcggg catcgccgac gagcgattcc
     1081 gcggcgtgac gccaagcgcg tgcatccttc ccgtcttcac gtgcggcagc gatgccggca
     1141 ccgacggaaa accggtctgc agccgacccg caacgagatc caccatcgcc gcgccctggc
     1201 ctttgaaagg cacgtgggta agccgcgtct gcgtcatcat ttgaaaaagt tcgcccgcga
     1261 ggtggctcgc cgaaccggtg ccggaagacc ccatgttgag ttcgcccggt ttggtgcgag
     1321 cgtaagcgat gaagtctttg accgagcgcg cagggatagc gggatgcacg acgagcacat
     1381 tcggcaccat cgccacaagc ccgatcgcgg agaagtcgtt caccgaatcg tatttcaacg
     1441 cgtacatcga tggactgatg gcgtgcccgg acggcacgat caggatcgta tggccgtccg
     1501 gtgtggcggt tgcagtaaga tgcgcggcaa tcgtctggtt cgcgccgggg cggttgtcca
     1561 cgatgacttg cgtgccgagc cgctcgccca ggcgctggcc gatggcgcgc gcgaggagat
     1621 ccgccgcacc gccgggcacc acgcctacga gaaagcggat cggcctcgcc gggtaaggcg
     1681 tttgcgccag cgacggaagc gcaccgcccg caaagctcaa taccaagcca agcgaggcta
     1741 ccttacgcgt gatcgctcgc atcgtcccct cccggcattc gcgcctattt tgcgagcccg
     1801 atgtccacca gcaccgcttt catcgcgtca tagtcgcgct cgagctcctt cgcaaaggca
     1861 tcgccggcag tgaacacgtt tacccagtag ttgcgctcga gatcctgttt ccactccggc
     1921 gcctccgtgg ccttgcgcag ggcgccggcc cagtacgccg cctgctcggg tgtgatgcct
     1981 ttcggcgcga aaatcgcgcg ccaggcgccg aacacgtgat ccacgccctg ctctttccat
     2041 gtgggaacgt gcgcgagcgc gcctgtgaaa cgctgagtcg ccgtaatggc cacgatattg
     2101 agcttaccgt tcgtcacgtg gcccgcaacg ttgccgaccg cagtcgtcac gagatcgata
     2161 tggccgccca tcaggttggg gatcgcatcg gcggacccct tgtaggccac cactttgaga
     2221 tcgcgcgcgt taccgccgat cgttttcatc aagaggccgg ccgcaatgtg gttgtggctg
     2281 ccgaggagcg gcgaaaagcc gatggtgacc gacttggggt ccttctgcag acgatcgatg
     2341 aggtccttgc cggatcggag cggcgtgcca gccgcgaccg cgaacgcagt gtagtcgtcg
     2401 aacagcgcgg cgatcggcgt gagatcactg tggcggagct tgctcgcgcc cgtgatgtga
     2461 ttggtgagga gcgccggccc ggcgaccacg aggtagtgcg gatcgccggc gtgctgctgc
     2521 acgtaagtgt aggcgatgct gccgccgccg cccggcttgt tgacgaccgt gagcgtggac
     2581 ggcacgagat tgttcgcctt cagaatgcgc tctacggtgc gcgcagtctt gtcgttgctg
     2641 ccgccgggcg gattgggcac gaccagctcg acgttcttct gcggcgtcca cccctgcgca
     2701 ccggccgcag cgcccatcgc cccgaacaac accgcaacga tacacttccc gattctcatg
     2761 ctcgctcctc tgattttcgc ttatgcggcg cgcgccgcat ggggacggaa taccgcaaac
     2821 actttatcgg agttggtaat ccacgactcc agaaaaaccg attcgtcggg ctctctgcgg
     2881 ccgagctcgt gcatcgcgcc cgcgagacag aaccagttga gcagctcgtg gtgtccgcgg
     2941 tcctcgactt cttcgagcgt cgtgttacgc cagtactgcc agtcgccgcg cttcagcgcc
     3001 tcgtaatagc tgcgatcggc ttcgacatcg gggaacatgt gcgaatgctt ttgcacgagg
     3061 aaggagtggc tccagctcga ggaggcgatc aaggcaacgc gccacgggct gtgtttcagc
     3121 gcacgcgcga ccgcggcacc gagctcgaag cagcgccacg gctgcggcgc aggcggatcg
     3181 agcgcgcttt ctccctccgc gagctgctca gccgcttccg agggacgcat cggcgccccg
     3241 cggccagcga tcagccagcg cccgtaggcg ttgatggcga acggcaccaa ggcatatgga
     3301 aatcccctgc ggtcgtagtc gaggtagagg atcgaattcg agaacgcgtg cccgagcggc
     3361 cgatggaggg gctggtaggc atacgcgaca tcaaatccga cgcggagcag cgcggtggcg
     3421 agatactttc cgccggcccg gtgtcccgcc accgtgaacg tctgatcttc cggctcatcc
     3481 cacgcgttcg gtccgcgccg gtaattcttc cacggctgga acgacacgcg atcgtaggca
     3541 aggatggcaa acgccggcac gcaatcctcg cggaaattct cgtactgatc gtcaccccac
     3601 atcagcacga aatcgggctt gaacgcatcc aattccgccc gcgcccagcg aaaccgctcg
     3661 accaggtcgc gccggtgacg gtccgaatgc gcctgtcctt cgtcgccgcc ccattgctcg
     3721 cgcatggtcg gatgccagtt ctgcggcgag cgcaatcgct cgtccagcgc agggtcggca
     3781 aggcactgct gcatgcgcca gctcatgttc cgttttgccg aaagattggg atagtgcgtg
     3841 atgcccagac cgaggatctc gcccatgtgt gctcctccgc gtgcccggac tgttggaaca
     3901 cccgccgggg atgagcattc tacgcgcctg gcatcggctc ggtctgccgc cggcacggcg
     3961 aaaaggtaaa ctcgcgccta aggtcgctgc gcaacggaag agctggacgg caaactgcgc
     4021 ggagtgctgg gcgatctcgg gctcctcaag tagcagggtt tgaacggaga taatgatggc
     4081 caacggactg aaaccgatac ggcgcgtggt caccggcaac gacgagcgcg gccgatcgaa
     4141 ggtcgtgtgg gacgggcccg cgcccaattc gcacgaagct tccatgggaa gcgggcgcgg
     4201 ccacaccgac ctttgggtgt ggaacgatac gcccgcgccg ctctccgggg cgagcgacga
     4261 cggaaatctg ccctacacct tctgcggccc gcagaacggc ggtcacttcc gcatcgtaca
     4321 ggcgcggccg cggaccgcgg actacagccc tgcgaaggac aaggaagcag tggcaccgca
     4381 tccgccgaag ctgcggcccg gcgggcgggg cacctgggat cgcggcggaa acaattcgta
     4441 ctcgtccgcg atgcacaaga cggaaaccgt cgactacggc atcgtcatgg cgggcgagcg
     4501 tggcctcata ctcgacgact gcgaactgac gatgaaaccg ggcgacatcg tgatccaggt
     4561 cggagcctgg catcagtggt ccatgccgaa gggcgcgatg atggccttcg acatgatcgg
     4621 cgcgcg
//
LOCUS       DASURG010000593         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_100609, whole genome shotgun sequence.
ACCESSION   DASURG010000593 DASURG010000000
VERSION     DASURG010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100609"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..789
                     /gene="mraZ"
                     /locus_tag="VFY80_10735"
     CDS             340..789
                     /gene="mraZ"
                     /locus_tag="VFY80_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6008431.1"
                     /translation="MQAGRGLGKFLGSYLYQLDDKGRVALPAAFRREAADQRFVLLQP
                     YPPALALYPEGEWLQVEERVRDLLKHQPEARMWVLRMMASAVEVTPDSQGRILVPARL
                     KEAAQLETQALLVGAIDKIEIWNPTLFESAATEEAEKFTQFAPKIFR"
     gene            849..1760
                     /gene="rsmH"
                     /locus_tag="VFY80_10740"
     CDS             849..1760
                     /gene="rsmH"
                     /locus_tag="VFY80_10740"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6008432.1"
                     /translation="MTEVYHVPVMVREVLTFLAPGRGGVYFDGTLGGGGHTAAILDAG
                     EAARVIAVDQDPEALAVAQARLAEYGDRVRFVRANFADAAEDDAEPLAGALLDLGISS
                     RHIDSPERGFTFRTGTPLDMRMSGRGRTAADLLNTLSEAELADIFYHFGEERRSRRLA
                     RDVVRTRKAQPFRTSDDLLAAMERALGRLEPQDKARIFQALRIAVNDELEALDRALPA
                     LRDRLENDGVLVVLSYHSLEDRRVKDAFREWSRSCICPPEVLVCQCRGRALGQTLTKK
                     PMPASDDEVAINSRARSAKLRAWKRVA"
     gene            1760..2104
                     /locus_tag="VFY80_10745"
     CDS             1760..2104
                     /locus_tag="VFY80_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008433.1"
                     /translation="MRSHAGTIRLALCFALLLCALTLVIWRQSDALATLRELDRVRAA
                     RALAEAERSDLLRDIEHLESRAVVVTAAGDRLGMRVPSVHEIVILPLQPKAAPQSPTS
                     SRANIALTMGRP"
     gene            2101..>3418
                     /locus_tag="VFY80_10750"
     CDS             2101..>3418
                     /locus_tag="VFY80_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6008434.1"
                     /translation="MSRKSPQATTRSAALRRRRWLLLGILIAAFGLTARAVKLQLVDA
                     AEWQARAEDQHAKQSVIPAPRGTIYDRDGVPLASSSEAFRVNIAPRDVKDRKQLAKRL
                     RTLTSLDQKTINRALNPERRWVVLPGRYDAAVRQALDGVAGVHFDRVQMRFYPRGTIA
                     RDILGAVNSEGEALSGVELEFDSLLAGEPGVTIVRRDPRGRALPGVMVQARPPEPGHD
                     IYLTIDSDLQEIAAEALRNAVRKTGARGGDLLLADPRTGEILASASVREKTADNWRAA
                     TEPYEPGSTLKPFLIATLLAEKQARLTDSVFAENGSYTNEGRTLRDVHAIGWTSVAGA
                     LHHSSNIALAKLSQRLEPEIQYQYLRSFGFGAPTAVTYPSESSGLLRRPANWSRFSQA
                     SLAIGYEISVTPMQMVLAYGALANGGLLMEPRLVREVRSRDARVTKK"
BASE COUNT          615 a         1032 c         1156 g          615 t
ORIGIN      
        1 tgtgcgggcg gcgcgcacgg cagatcgaag ttcgtgggtg acggctgcgt cggatgtttt
       61 ggcgatcagg gcaattctca ccggctctcc ggacgtcggt ttacttctga acatttgacg
      121 catacgcaca tgcagtgctc agccggcttc cccggggaaa ccgttgagct caaatcggcg
      181 cgtatgcttg gctgcgtcaa aagttcagaa gtaaaccgac gtccgaatgc cgctcttgac
      241 tcgctgtggg cccgaccggt actattgtgg cgccgagtgg ggaatagtgg ggaagattgg
      301 tggaaggtcc ctcggcttga cctcgaccgg tggcagcaca tgcaggcagg cagggggctg
      361 gggaagttcc tcgggagcta tctctatcag ttggacgata aggggcgtgt cgccctgccc
      421 gcggcgtttc gccgcgaggc ggcggatcag cgcttcgtcc tgctgcagcc gtacccgccc
      481 gcgctggccc tctacccgga aggcgagtgg ttgcaggtgg aggagagggt ccgcgacctg
      541 ctcaagcatc agccggaagc gcgcatgtgg gtgctgcgca tgatggccag tgcagtggaa
      601 gtgacgccgg acagccaggg ccgaatcctg gtgccggcgc gattgaagga agcggcccaa
      661 ctcgagacac aagcgctgct ggtcggagcg atcgacaaga tcgagatctg gaatccgacg
      721 ttgttcgagt cggcggcgac agaggaagcg gagaagttca cacagttcgc accgaagatt
      781 ttcagatagc acggcagagt tgggcgcggg cgagtactcg tgcccggact tgaaaacgaa
      841 agagttctgt gaccgaagtt taccatgtcc ccgtcatggt gagagaggtc ctcacctttt
      901 tggcaccagg ccgaggtggc gtctatttcg acggcacttt aggtggcggc ggacatacag
      961 cagcgattct ggacgcaggt gaagcggccc gcgtcatcgc ggtcgaccag gacccggaag
     1021 ccctcgcggt ggcgcaggcg cgcctcgcgg aatacggcga ccgggtccgg ttcgtccggg
     1081 cgaatttcgc cgatgcggcg gaggatgatg cggaaccgct ggccggcgcg ctgctcgatc
     1141 taggtatttc atcccgtcat atcgattccc cggagcgagg tttcaccttt cgcacgggta
     1201 cgcccttgga tatgcgcatg agcggtcgcg gtcggaccgc ggcagatctg ctgaacacgc
     1261 tctccgaagc cgagctggcc gacatcttct accattttgg tgaagagcgt cgctcgcggc
     1321 gtttagcgcg cgacgtcgtc cgcacgcgca aagcgcagcc cttccgtacc agcgacgatc
     1381 tgctggcggc gatggagcga gcgctcggtc ggctcgagcc gcaggacaaa gcgcgcatct
     1441 tccaggcact gcggattgcg gtcaatgacg agctggaagc gctcgaccgg gccttgccgg
     1501 ctctgcgcga tcgcctggag aacgacggtg tgctggttgt gctgtcgtat cactccctgg
     1561 aagatcgccg cgtcaaggac gcgttccgcg agtggagccg cagctgcatt tgtccgccgg
     1621 aagtccttgt ctgtcagtgc cgcgggcggg cgctcgggca aactctcacg aaaaagccga
     1681 tgccggcgag cgacgacgag gtcgcaatca actcgcgagc ccggagcgcg aagctgcgcg
     1741 cgtggaagag agtcgcctga tgcgttcgca cgccggcacc atccgactgg cactttgctt
     1801 cgcgctgctg ttgtgcgcgc tgacgctcgt catctggcgc cagagtgacg cgctggcgac
     1861 gctgcgtgag ctggatcgcg tgcgcgctgc gcgtgcgctg gcggaggcgg agcgctcgga
     1921 cctgctgcgg gacatcgaac acctggaaag ccgggccgtg gtcgtaaccg cggcgggtga
     1981 tcggctgggc atgcgcgttc caagtgtgca tgaaatcgtg atactgccgc tgcaacccaa
     2041 agcggcaccg caaagcccaa cctcgagtcg cgccaacatc gcgcttacca tggggcggcc
     2101 atgagcagga aatcgcccca ggccaccacg cgctcggccg ccctgcgacg tcgtcgttgg
     2161 ctattactcg gcattctgat tgccgctttc gggctgactg cgcgagccgt caagcttcag
     2221 ctggtggatg cggctgaatg gcaggcgcgc gccgaagatc aacacgccaa gcagtcggtc
     2281 attccggcac cgcgcggtac catctatgat cgggatggcg tgcctctggc atcgagcagc
     2341 gaagccttcc gcgtcaacat agcgccgcgt gacgtcaaag atcgcaaaca gctcgcgaag
     2401 cggctgcgta cactgacgtc gctggatcag aaaacgatca accgcgcgct caatcctgag
     2461 cgcaggtggg tcgtgctgcc gggccgctac gatgcagccg ttcggcaggc gctggatggc
     2521 gtcgcaggtg tccacttcga tcgcgtgcag atgcgtttct atccacgcgg caccatcgct
     2581 cgcgacatcc tcggtgcggt caactccgaa ggcgaagcac tcagcggcgt cgagttggag
     2641 ttcgattcgc tgctggccgg cgagcccggc gtgacgatcg tgcgacggga cccgcgcggc
     2701 cgcgcgcttc cgggtgtcat ggtgcaggcc cgtccgccgg agcccggtca cgacatctat
     2761 ttgaccatcg attcggatct gcaggagatc gcggccgagg cgctgcgcaa cgcggtgcgt
     2821 aagaccgggg cgcgcggtgg tgatctgttg ctggccgatc cacgcaccgg cgagatcctc
     2881 gcttcggcaa gtgtgcgtga aaaaacggca gacaactggc gtgcggcgac ggagccgtac
     2941 gagcccggct ccacgctcaa accgttcctg attgcaacgc tgctggcgga aaagcaggcg
     3001 cggctgaccg acagcgtatt tgccgaaaac ggcagctata cgaatgaagg gcgcacgctg
     3061 cgcgatgtgc atgccatcgg ctggacctcg gtcgcgggcg cgctgcacca ctcctcgaac
     3121 attgcgctgg ccaagctctc ccagcgcctc gaacctgaga tccagtacca gtaccttcga
     3181 tcgttcgggt tcggcgcgcc cacggccgtg acttatccct cggaatcgag cggactgttg
     3241 cggaggccgg ccaactggtc gcgtttttcg caagcgagcc ttgccattgg ttacgaaatt
     3301 tccgtcacac ccatgcaaat ggtgttggct tacggcgcgc tggcgaacgg cggcctgctc
     3361 atggagccac ggctggtacg agaggtgcgc tcacgggacg cacgggtaac gaagaaat
//
LOCUS       DASURG010000594         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_713916, whole genome shotgun sequence.
ACCESSION   DASURG010000594 DASURG010000000
VERSION     DASURG010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713916"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VFY80_10755"
     CDS             complement(<1..1317)
                     /locus_tag="VFY80_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6008435.1"
                     /translation="MTTSAQTLSEFVVRLHYEDIPAEVIERAKDCVIDTVAACVFGAE
                     LPWTKTVIAYAERNSAPGRCSVLGTPIKVRAPFACLSNGASAHAFELDALCEPSVGIH
                     PSAALAVPGLAVSQGRRKSGREFLAAVVAGYELLYRIGDAARHSIEKVGFHSPGVVGV
                     YGTAAVVGRLFDIDARQMANAFGIAGSMSSGLMEFSRSGGMVKRLHLGRAAEGGFMAA
                     ILARDGYTGPSEVFEGKFGLLNVYCREADPSRLTKALGTAWHVMKTKIKRYACHATAQ
                     VPVTLALELKAKHGLSGEDVAHIAIAANEKTVTQHAINEPRDMTMMQYSVPFAVAVAL
                     YRDPTDPRVFSEATLNDAKIRALAKTAEVRSLEASGARMSSTARVTLRLRNGQSVSAE
                     GHDFKGTPTMPLTRDELSEKFLKLTAHRDRAKAEHLFSQLAEAEKLS"
     gene            complement(1314..2294)
                     /locus_tag="VFY80_10760"
     CDS             complement(1314..2294)
                     /locus_tag="VFY80_10760"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008436.1"
                     /translation="MDRCLAIISCAVLFFSVSAAHGAPPAWKPVKNIEIIIPGGVGGG
                     QDRTARILQKIMQDGIVPVSVTVVNRPGGGGNIAYTYLNQFTGDGHHLATATATLLTN
                     NILGVSPLNYTHFTPLSVLYGEYIGFATQADGPLKTGRDIVERVRKAPESVSFAFGTS
                     RGNANHIGIALAMKAAGIDPATLKIVVYKASIEATTALMGGHVDVVATPMSTYLPVLG
                     SGKIRIVAIAAPQRVGGRFADVPTWKEQGFDAVMPSYRMFIAARGLDAAQRRYWDGVF
                     ARIAKSPEWQKELAANEWQSAYMNGADSVKYLEARYAEYRRILTELKLAK"
BASE COUNT          413 a          788 c          743 g          461 t
ORIGIN      
        1 ggagagcttc tccgcctccg cgagctgcga gaaaaggtgc tcggccttcg cgcgatcgcg
       61 atgtgcggtc aatttgagaa atttctcgct cagctcatcg cgagtgagcg gcatcgtcgg
      121 tgtgcccttg aagtcgtgtc cctccgccga aaccgattgg ccgttcctga gccgtagagt
      181 aacgcgcgcg gtggacgaca tgcgagcacc gctcgcttcg agcgaccgca cttcggctgt
      241 tttcgcgagc gcacggatct tggcatcatt cagggtcgct tccgagaata cccgggggtc
      301 ggtcggatcc cggtagagtg cgacggcgac cgcgaacggc acgctgtatt gcatcatcgt
      361 catgtcccgc ggctcgttaa tggcgtgctg ggtcacggtc ttttcattcg ccgcaatggc
      421 aatatgtgcg acgtcttctc cggacaggcc atgcttcgcc ttcagctcga gcgcaagcgt
      481 caccggcacc tgcgccgtgg catggcatgc gtagcgcttg atcttggtct tcatgacgtg
      541 ccatgcagtg ccgagcgcct ttgtgagccg cgatggatcg gcttcacgac agtagacgtt
      601 gagcaagcca aacttgcctt cgaacacctc gcttggcccc gtgtagccgt cgcgcgcaag
      661 gatggccgcc atgaagcccc cctcggccgc acgtccgaga tggaggcgct tgaccatccc
      721 cccggagcgc gaaaattcca tgagtccaga gctcatcgag cccgcgatgc cgaacgcgtt
      781 cgccatttgc cgggcatcga tgtcgaagag cctgcccacc accgcggcag tcccgtagac
      841 gcccaccacg ccgggcgagt ggaaaccgac tttttcgatc gaatgacgcg cagcgtcccc
      901 gatgcggtag agcagctcgt atccggcgac gaccgccgcc agaaattccc ggccgctctt
      961 ccggcggcct tgcgatacgg cgagtcccgg caccgcgagc gccgcgctgg ggtggatgcc
     1021 gacgctcggc tcgcacagcg catcgagctc gaacgcgtgc gccgaggcgc cattcgacag
     1081 gcacgcgaac ggcgcccgca ccttgatcgg cgtgccgagc acgctgcacc gccccggggc
     1141 gctgttcctc tcggcatatg cgatgacggt tttcgtccag ggcaattccg cgccgaacac
     1201 gcacgcggcc accgtgtcga tgacgcagtc tttcgcgcgc tcgatcacct cggcgggaat
     1261 gtcctcgtaa tgcagtcgaa cgacaaattc ggaaagggtc tgcgcagagg tggtcatttt
     1321 gcaagcttga gttcggtcag tatccgtctg tattccgcgt agcgcgcttc gaggtacttc
     1381 acgctgtccg cgccgttcat atacgcgctt tgccattcgt tcgccgccag ctccttctgc
     1441 cattccgggc tttttgcgat gcgcgcgaac acgccatccc agtagcgacg ctgagcggca
     1501 tcgagtccgc gcgccgcaat gaacatccgg tagctcggca tcaccgcatc gaagccctgc
     1561 tccttccacg tcgggacgtc ggcaaagcgc ccgccgaccc gctgcggcgc cgcgatcgcg
     1621 acgatgcgga tcttgcccga gccgagcacc gggagatagg tggacatcgg cgtcgcgacc
     1681 acatcgacgt ggccgcccat gagcgcagta gtggcctcga tggacgcctt gtagacgacg
     1741 atcttgaggg tcgcggggtc gatgccggcc gccttcatcg caagcgcgat gccgatgtga
     1801 tttgcgttgc cgcggcttgt cccgaacgcg aaggagaccg attcgggcgc cttgcgcacg
     1861 cgctccacaa tgtcccgacc ggtcttcagt ggtccatcgg cctgcgtcgc aaaaccgatg
     1921 tactcgccgt agagcacgct caagggcgtg aagtgcgtgt agttgagcgg actcacgccg
     1981 agaatgttgt ttgtgagcag cgtcgccgtc gccgtcgcga gatggtgtcc atcgcctgtg
     2041 aactggttga ggtaggtgta cgcaatattc ccgccgccgc ccggacggtt caccaccgtg
     2101 acgctgaccg gcacgatgcc gtcctgcatg atcttctgga gaatgcgcgc ggtgcggtcc
     2161 tggccgccgc ccacgccgcc cggaatgatg atttcgatgt tcttgactgg cttccacgcc
     2221 ggtggcgcac catgagcagc gctcacggaa aagaacagca ccgcgcatga gatgatcgcc
     2281 agacatcggt ccatacgggc ctcgccttcc tctcgccagt ggacggcatt ctaggctcaa
     2341 ttccggagcg cgcacaatga agcacgcgtc ggttcgcggc gcccatgtgc taaaattcgc
     2401 ctgca
//
LOCUS       DASURG010000595         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_407784, whole genome shotgun sequence.
ACCESSION   DASURG010000595 DASURG010000000
VERSION     DASURG010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407784"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VFY80_10765"
     CDS             complement(<1..96)
                     /locus_tag="VFY80_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX6008437.1"
                     /translation="MTQELWLRDPTAETTPLRRPRTTPPKSLEGLT"
     gene            complement(93..1175)
                     /locus_tag="VFY80_10770"
     CDS             complement(93..1175)
                     /locus_tag="VFY80_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008438.1"
                     /translation="MAEKLEAKYAGGTALASRKVSYGEHDDPMEVCFARGWTDGLPVT
                     PPTDERIIRMLKGTSRVPGEVIGRIPPYLAECTIEKVAINAVLAGCRPEYMPVLLAAL
                     EAALEPVFTLHGVLATTYFSSPIVIVNGPIAKRIGMNSGLNALGQGNRANATIGRVLN
                     LIVQNVGGGRPGEADRSTLGAPSKFTCCFAEDESDAEWQPLSVARGFPRGTSTVTLFQ
                     GHGVEAFVDQKSRTPEALTRSLAMSLVKIGHPKLVQSARAILVLSPEHYAIYREGGWD
                     RARIERALFEATIRPGKEIVAGADGVGEGIPASRAGEQVPKFHEDGLMVVRAGGSAGL
                     FSAILPGWLAGRKREELQPVTKEVRA"
     gene            complement(1200..1532)
                     /locus_tag="VFY80_10775"
     CDS             complement(1200..1532)
                     /locus_tag="VFY80_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX6008439.1"
                     /translation="MITDGVVAFVKRACPTCTLIEAEMRELAGRMTEFSVVSQDDPGF
                     PGGVTRVVDDRELEHSWRNQIEATPTLIRFSGGRETDRVVGWDREGWRRLTGVADLGA
                     SLPAFRPG"
     gene            1608..2564
                     /locus_tag="VFY80_10780"
     CDS             1608..2564
                     /locus_tag="VFY80_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6008440.1"
                     /translation="MSIRDDSNAGRSLAPPRRRTLKLLAGGFLAMSQPLSSVAAKPGV
                     LLTRPIPRSGEALPIVGLGTWQTFDVGPNAPERAELKEVLRLLAAQGASVVDSSPMYG
                     QAERVVGDLAAELALHQKLFLATKVWTSGRDAGIRQMENSFRLLRTQRIDLMQVHNLL
                     DLTTHIKTLREWKKAGRIRYIGITHYHEGAHRDLERLVKTRDYDFVQFNYSMTEREAE
                     ERLLPACQDSGTAVLVNRPFAQASLFGRVKGKPLPAWAAEFDCASWAQFFLKYLLGHP
                     AVTCVIPGTRRTTHLRDNLAAGMGRLPDVAMRKRMVAHLEQL"
     gene            2640..3533
                     /locus_tag="VFY80_10785"
     CDS             2640..3533
                     /locus_tag="VFY80_10785"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEX6008441.1"
                     /translation="MTRFCTLAALRWAVLAISLVVLPGCSMMRLGYGYLDTYAAWKAN
                     EYFDLESHQKDDFQRRFDRLHEWHRYQELPDYAAFLAKARQRLDKSIEPQDVDWFVDG
                     VKERYARIVSRATDDAVVLLYTLTPAQLEVLKQRWDKDNRRFVREYRLNGSPDDIKRA
                     RARRTLHQIREWVGPLSHEQEHQVIAATDAMPMTEKLRYEDRVRRQREFLRLMAERSE
                     DREAFAQKLRAWLIDWNKGRDPEYERLARRVYAERVKLVIELERTITPHQRALALGRL
                     QDYIDDFTRLAQRPHGRTAAN"
     gene            3511..>3735
                     /locus_tag="VFY80_10790"
     CDS             3511..>3735
                     /locus_tag="VFY80_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008442.1"
                     /translation="MAGLRRTEVRLRGKAALLQYRRALVTRAPMPAYSRPIASKLPQV
                     GTTIFTVMSRLASEHNAINLSQGFPDFDCAA"
BASE COUNT          685 a         1257 c         1153 g          640 t
ORIGIN      
        1 ggtaagtcct tcgagcgatt tcggcggcgt cgtccgcggc cgcctgagcg gcgtcgtttc
       61 ggctgtcgga tcgcgcagcc acaattcttg cgtcatgctc gcacctcctt ggtcacgggt
      121 tgcagttcct cgcgcttgcg cccggcgagc catcccggaa ggatggcgga gaagagtccc
      181 gccgatccgc cggcgcgcac gaccatcaat ccatcttcgt ggaacttggg cacctgctcg
      241 cccgcacggc tcgcgggtat gccttcgccg accccatccg cgccggccac gatctccttc
      301 ccggggcgga tcgtcgcctc aaaaagcgcg cgctcgatgc gcgcgcggtc ccatccgcct
      361 tcgcggtaga tcgcataatg ctccggcgag agcacaagta ttgcacgggc ggactgcacg
      421 agcttgggat gtccgatctt gacaagagac attgcgagcg agcgcgtgag cgcctcgggc
      481 gtgcgcgact tctggtcaac gaacgcctcc acgccgtgtc cctgaaacag cgtgacggtc
      541 gacgttccgc gcggaaagcc acgcgcaacc gacaacggct gccactccgc atcggattcg
      601 tcctcggcga agcagcacgt gaacttgctc ggcgcaccga gcgtcgagcg atccgcctcg
      661 ccgggacgcc ctccgcctac gttctgcacg atgagattga gcacgcgtcc gatggtcgca
      721 ttcgcgcggt tgccctgccc cagcgcgttc aatcccgaat tcatgccaat ccttttcgcg
      781 atcgggccgt tcacgatgac gatcggcgac gagaagtagg tcgtggccag tacgccatgc
      841 aacgtgaata ccggctccag cgccgcttcc agcgcggcga ggagtaccgg catgtattcc
      901 ggccggcagc cggcgagcac cgcgttgatc gctacctttt cgatcgtgca ttcggcgagg
      961 tacggcggga tgcgcccgat cacctcgccc ggtacgcggc tcgtaccctt cagcatgcgg
     1021 atgatgcgct cgtcggtcgg cggcgtgacc ggcagtccgt cggtccaacc gcgggcgaaa
     1081 cagacttcca tcggatcgtc gtgctcgccg tacgagacct tgcgcgaggc gagcgccgtg
     1141 ccgccggcat acttcgcttc cagcttctcg gccaccccgg gctcgagcgt gagcgatcct
     1201 caccccgggc gaaacgccgg caggctcgcg ccgagatctg caacgcccgt cagccggcgc
     1261 cagccctcgc ggtcccaacc gacgacgcga tcggtctcgc gtccgccaga aaagcggatg
     1321 agcgtgggcg tcgcctcgat ttggtttcgc cacgaatgct cgagttcgcg atcgtccacg
     1381 acccgcgtca cgccgcctgg aaaccccggg tcgtcctgcg acacgacgct gaattccgtc
     1441 atcctgccgg cgagctcgcg catctccgct tcgatcaggg tgcaggtggg acacgcccgc
     1501 tttacgaatg caactacgcc atcggtgatc atgcggctta cccgagcgat tgagacaacg
     1561 ctttcgattc taacgcctct tcaggcagcg tgataagctt cgggcacatg agcatccgcg
     1621 acgattcgaa tgctggtcgc agtctcgcgc ctccccgccg gcgcacgctc aagctcctcg
     1681 caggaggatt tcttgccatg tcgcagccgc tttcatcggt cgccgcgaag ccaggcgtcc
     1741 tgttgacgcg ccccattcca cgaagcggcg aagcgctgcc catcgtgggg ctcggcacgt
     1801 ggcaaacctt cgacgtcggg ccgaatgcac ccgagcgagc agaactgaag gaagtgctgc
     1861 gccttctcgc cgcacagggg gcaagcgtcg tggactcctc gccgatgtac ggacaggccg
     1921 agcgcgtcgt gggcgatctt gctgccgagc tcgcgctgca tcagaagctc ttcctcgcga
     1981 cgaaagtatg gaccagcggg cgcgacgccg gcatccgcca gatggagaat tcgttccggc
     2041 tgctgcgcac gcagcgcatc gatctcatgc aggtccacaa cctgctcgac ctcaccacgc
     2101 acatcaagac gctgcgcgag tggaaaaagg ccgggcgcat ccggtatatc ggcatcactc
     2161 actaccatga gggcgcgcac cgcgacctcg aacggctggt aaagacgcgc gactacgact
     2221 tcgtgcagtt caattattcg atgaccgagc gcgaggccga ggaaaggctt ctgccggcct
     2281 gccaggacag cggaacggca gtgctcgtga accggccatt tgcacaggcg agccttttcg
     2341 ggcgggtcaa aggaaaaccg cttccggcat gggcggcgga gttcgattgc gcgagctggg
     2401 cgcagttctt cctcaagtac ctgctgggac accccgcggt cacctgcgtg ataccgggaa
     2461 cgcggcgcac gacgcacctc agggacaacc ttgcggccgg catgggccgg cttcccgacg
     2521 tggcgatgcg caagcgcatg gtggcgcacc tcgagcagct atgactcacc ggtcgtaaga
     2581 gcccgatccc gtatgcgctc ggttcgaaac cttggcgggc ggcgggttac aaagacacta
     2641 tgacgcgatt ctgtactctc gccgcactgc gttgggcagt gctcgccata agcctggtcg
     2701 tgctccccgg ctgcagcatg atgcgcctgg gatacggcta tctcgacacc tacgctgcgt
     2761 ggaaggcgaa cgaatatttc gatctcgaat cccaccagaa agacgacttc cagcgccgct
     2821 tcgaccggct ccatgaatgg caccgctacc aggagcttcc cgattacgca gctttcctcg
     2881 caaaggcgcg ccagcggctg gacaagtcca tcgagccgca agacgtggac tggttcgtcg
     2941 acggcgtcaa ggagcgttac gcgcggattg tctcccgtgc aaccgacgat gcggtggtgc
     3001 tgctctatac cctcacgccg gcgcagctcg aagtgctcaa gcagcgatgg gacaaagaca
     3061 accgccggtt cgtgcgcgaa tatcggctga acggcagccc ggatgacatc aagcgcgcac
     3121 gggcgcgacg cactctgcac cagatccgcg aatgggtggg tccactgagc cacgagcagg
     3181 agcaccaggt tatcgccgca actgatgcca tgccgatgac cgagaaactg cgctatgaag
     3241 accgcgtgcg ccgtcagcgc gaattcctgc gcctcatggc cgagcgcagc gaggaccgcg
     3301 aagcattcgc gcagaaacta cgcgcgtggc tcatcgattg gaacaagggc cgcgacccgg
     3361 aatacgagcg gctggcgcgc agagtgtatg ccgagcgcgt gaaactggtg attgaactcg
     3421 agcgcacaat cacgccccac cagcgcgcgc tcgcgctggg gcgcctccag gattacatcg
     3481 acgacttcac acggctcgcg cagcggccgc atggcaggac tgcggcgaac tgaagtgcgc
     3541 ctgcgcggga aggccgcgct gctacaatac cggcgcgccc tcgtgacccg tgcacccatg
     3601 ccggcctact cgcgacccat cgcatcgaaa ctgccgcagg tcggaacgac gatcttcact
     3661 gtgatgtctc gtcttgcgag cgagcataac gcgatcaacc tttctcaagg ttttcccgac
     3721 ttcgattgcg ccgcc
//
LOCUS       DASURG010000596         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_918286, whole genome shotgun sequence.
ACCESSION   DASURG010000596 DASURG010000000
VERSION     DASURG010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918286"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFY80_10795"
     CDS             complement(<1..242)
                     /locus_tag="VFY80_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008443.1"
                     /translation="MELFSALVDIVLHLDRHLQWLVANYGVWIYAILFLIIYCETGLV
                     VTPFLPGDSLLFVAGTLAATGDMYIHGLFGLLVLAS"
     gene            complement(253..1191)
                     /gene="secF"
                     /locus_tag="VFY80_10800"
     CDS             complement(253..1191)
                     /gene="secF"
                     /locus_tag="VFY80_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX6008444.1"
                     /translation="MELFRIKRDIPFMRHALIFNVISIVTFLIAVGSLATRGLNLGVD
                     FTGGTLMEVSYAQPADLQKIRETAAKLGFSEASVQNFGTASDVLIRLPVKKDLTSAKL
                     SELIMAELRKDDPSVQMRRVEFVGPQVGSELLYNGSLALLFVSLGIVGYLAFRFEWKF
                     GVATIVANLHDIVIILGFFSLFQWEFSLPVLAAVLAILGYSVNESVVVFDRVREVFRN
                     RRWRSRTVPEVLDHAITATMSRTIITHFSTQLMVCSMLVFGGETLFYFALALTIGILF
                     GIYSSVLVAAPIVMWLGVSREDLARPERKAEPEAVV"
     gene            complement(1218..>2483)
                     /gene="secD"
                     /locus_tag="VFY80_10805"
     CDS             complement(1218..>2483)
                     /gene="secD"
                     /locus_tag="VFY80_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6008445.1"
                     /translation="SEFRVVGTLKPEAQRQAEGFALEQNITTLRNRVNELGVAEPIIQ
                     QQGADRIVVQLPGVQDTAKAKDILGRTATLEVRMVEAHSSDPGVRDYDPQKIQQALAG
                     QVPFGTELFFLQRDGGKEPLLLSRQVVITGDRLTDASPGFDQTDNRPIVSVTLDGQGA
                     RIFQQVTREAVKRRMAILLVDRGKPEVLTAPVIQSELGARFQITGFRSTEETKDLALL
                     LRAGALAAPMEIVEERTVGPSLGAENIRIGFYSALYGFMAISVFMIVYYHMFGVFSVL
                     ALAANVLFLVALLSMLQATLTLPGMAGIALTIGMAIDANVLINERIREELRNGNTPQA
                     AIHAGYDRAWATILDSNLTTLIAGIFLFLLGSGPVKGFAVVLCLGILTSQFSAVVVAR
                     SMSNFYYGRRRKLDRISIGQVWRPATDKR"
BASE COUNT          499 a          786 c          780 g          418 t
ORIGIN      
        1 aaggatgcca gcacgagcag cccgaaaaga ccgtgtatgt acatgtcgcc ggtggccgcg
       61 agcgttccgg cgacgaaaag cagcgaatcg ccggggagga acggcgtgac cacgaggccg
      121 gtctcgcagt agatgatcag aaacaggatc gcgtagatcc agacgccgta gttcgccacc
      181 agccactgca ggtggcgatc gagatggagg acgatatcga ctaaggcgct gaaaagttcc
      241 acccgaagcc gcctacacga ccgcctcggg ttctgccttg cgctcgggcc tggcgaggtc
      301 ctcgcgcgag acgccgagcc acatcacgat cggggcagcg accagcacgg acgaatagat
      361 gccgaacagg atgccgatcg tgagcgccag cgcgaaatag aacagcgtct cgccgccgaa
      421 cacgagcatg gagcacacca tcaactgggt ggaaaaatgg gtgatgatgg tgcgcgacat
      481 ggtggcggtg atggcgtgat cgagtacctc cggcacggtg cggctgcgcc acctgcggtt
      541 gcggaatacc tcgcgcacgc ggtcgaacac cacgaccgac tcgttgacgg agtagccgag
      601 tatcgcgagg accgccgcga gcaccggcag cgagaactcc cactggaaca gtgaaaagaa
      661 acccaggatg atcacgatgt cgtgcaggtt ggcgacgatg gtcgccaccc cgaatttcca
      721 ctcgaaccgg aacgccagat acccgacgat cccgagcgac acgaagagaa gcgccaggga
      781 gccgttatag agaagctcgc tcccgacctg cggccccacg aactcgacgc gtcgcatctg
      841 aaccgacggg tcgtccttcc tgagttccgc catgatcagc tccgaaagct tggcgctggt
      901 caggtctttc ttcacgggga gccggatcag cacgtcgctc gcagtgccga agttctgcac
      961 gctcgcttcc gaaaagccga gtttcgccgc cgtttcgcga atcttctgaa gatcagccgg
     1021 ctgcgcgtaa gacacctcca tcagggtgcc gcccgtgaag tcgacgccga ggttcaggcc
     1081 gcgggtggcg agcgacccta ccgcgatcag gaacgtgacg atggagatca cgttgaagat
     1141 cagcgcatgg cgcatgaacg ggatgtcgcg tttgatgcgg aataactcca taaactatcg
     1201 gcctctgccc gctgtgttca tcgtttgtcc gtggccgggc gccagacctg gccgatcgag
     1261 atccgatcga gcttgcgacg gcgcccgtag tagaagttcg acattgaccg cgcgaccacg
     1321 accgcgctga actgggacgt caatatcccg agacacagca cgaccgcgaa tccctttacc
     1381 ggccccgacc ccagcagaaa caggaatatc ccggcaatca gggtcgtcag gttcgagtcc
     1441 agaatcgtcg cccaggcccg gtcgtacccc gcgtgaatcg cggcctgcgg tgtattgccg
     1501 tttctcaatt cctcccggat gcgctcgtta atgagcacgt tggcatcgat cgccatgccg
     1561 atggtgagcg cgatgcccgc catgccaggc agcgtgagcg tcgcctgaag catggagagc
     1621 agcgcgacga gaaagagcac gttcgctgcg agcgcgagga cggagaacac tccgaacatg
     1681 tggtagtaca cgatcatgaa gaccgatatc gccatgaatc cgtagagcgc cgagtagaag
     1741 ccgatgcgga tattctcagc cccgagggac gggccgaccg tgcgctcctc gacgatctcc
     1801 atcggcgcag cgagcgcgcc cgcgcgcagc agcaatgcga gatccttcgt ttcctcggtg
     1861 ctgcggaagc cggtgatctg gaagcgcgcc ccgagctcgg actggattac aggtgcggtc
     1921 agcacttccg gcttgccgcg atcgacgagc aggatcgcca tgcgccgctt gaccgcctcg
     1981 cgcgtcacct gctggaagat gcgcgcgccc tgcccgtcga gcgtcacgct cacgatcggc
     2041 cgattgtcgg tctggtcgaa acccggcgag gcatcggtca ggcgatcccc ggtgatgacc
     2101 acctgccggc tgagcaagag cggctccttg ccgccatcgc gctgcaggaa gaagagctcc
     2161 gtcccgaacg gcacctgccc ggccagggcc tgctgaatct tctgtggatc gtagtccctc
     2221 acgccagggt cgctcgagtg cgcttccacc atccgcactt cgagcgtcgc ggtgcggccg
     2281 aggatgtctt tcgctttcgc ggtgtcctgc acgccgggca actggacgac gatgcggtcg
     2341 gctccctgct gctggatgat cggctcggca acgcccagtt cattcacgcg gttcctgagc
     2401 gttgtgatgt tctgctccag cgcgaaccct tcggcctgcc gttgcgcttc gggcttcagc
     2461 gtgccaacga cgcggaattc gct
//
LOCUS       DASURG010000597         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_816046, whole genome shotgun sequence.
ACCESSION   DASURG010000597 DASURG010000000
VERSION     DASURG010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816046"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFY80_10810"
     CDS             complement(<1..620)
                     /locus_tag="VFY80_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6008446.1"
                     /translation="MSGILSGLPTTKYSLPGDAGYEKARTVWNAMIDRRPACIVHCAS
                     ADDVAGALRLARQHELQISVRGAGHNIAGTAVCEGGLMIDLSPMKAVRIDAEKKRAWV
                     APGVTLAEFDAAAQQHALATPVGINSTTGISGLTLGGGFGWLTRKHGLTIDNLLSAQM
                     VTARGEKLTLSESENPDLFWAIRGGGGNFGVVTEYEYRLHSVGPEIL"
     gene            909..>2003
                     /locus_tag="VFY80_10815"
     CDS             909..>2003
                     /locus_tag="VFY80_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6008447.1"
                     /translation="MYDRQSDTEILEGAVDVTLSEEHKRWRAVAQEFAEKVIKPDAPR
                     RDRLPTAAERIPWDWIREADRLGIRTLGVPKKYGGAGADILTLCLVGEELAVGDLGFA
                     VIMDQCWKMAHILSDAMTPEQQERFIPAFVKDPEATFAIGFTETGAGSDHQGYYDSPD
                     IAFHTTAVRDGDSYVINGAKRYVSNGCMAKLYFVVARTDTKKSLREGGSVFIVPSDTP
                     GFRAGSFHEKSSQRLATNGTFHFENCRVPASHLLGGEGLMSRLRADYMWGSKAEAAAT
                     ALGVGRAAYEYALAYAKERVQGGKPIVELAPVAMMLAQMAMKIDAARTQIWRAAWLAE
                     RRQPEARTLGLLAKVNASETAFEACKLACEI"
BASE COUNT          369 a          638 c          654 g          342 t
ORIGIN      
        1 agaatttccg gtccgacact gtgaagacgg tattcgtact cggtgacgac gccgaagttg
       61 ccgccgccgc cgcggatcgc ccagaagaga tccgggttct cgctctcgct caacgtcagc
      121 ttctcgccgc gcgcagtcac catctgagcg gagaggagat tgtcgatggt caggccatgc
      181 ttgcgcgtga gccagccgaa gccgcccccc agcgtgagcc ccgagatgcc ggtcgtggaa
      241 ttgatgccga ccggtgtggc gagcgcgtgc tgctgcgccg ccgcgtcgaa ctcggcgagc
      301 gttacaccgg gtgcgaccca cgcgcgtttt ttctccgcat cgatgcgcac cgctttcatc
      361 ggcgaaaggt cgatcatcag cccgccttcg cataccgcag tgccggcgat gttgtggcct
      421 gcgccgcgca cggaaatctg aagctcgtgc tgccgcgcga gccgcaaggc gccggccacg
      481 tcatcggcgc tggcgcaatg aacgatgcac gcaggacgcc ggtcgatcat cgcattccag
      541 acggtgcgcg ccttttcgta tcccgcatct ccgggaagcg agtatttcgt tgtcggaagt
      601 ccactcagga taccgctcat gtccttctgc actatcgatg ccatgactct cctccttgaa
      661 gtaagtgacg cgacaccaga acggtcaccc atcctagcgc gccacgcata aacgcatctc
      721 gtccaaacgg ctcatgcaat tccacactgg ccgcccacgc cgccgcgctg aagacggctc
      781 gaactgcgcg acggagagta tcgcgaacgt gcatcgtgcc tgtgtcggcg cggtttcgga
      841 ttgttgcggc tttgtatcat cgatcgagcg ccggcgccgg atttgcagag cgaatcagcg
      901 agttggctgt gtatgatagg caatccgata ccgagatcct ggagggcgca gtggatgtca
      961 ccctgtccga agaacacaaa cgctggcgcg cggtcgcgca ggaattcgcg gagaaggtga
     1021 tcaagcccga cgcgccgcgg cgcgaccggc tgccgaccgc ggcggaacgc ataccgtggg
     1081 actggatacg cgaggcggac aggctcggca tccgcacgct cggcgtgcca aagaaatacg
     1141 gcggcgcggg cgccgacatc ctgaccctgt gcctggtcgg cgaggagctc gcggtcggcg
     1201 atctcggatt cgcggtcatc atggaccagt gctggaagat ggcgcacatc ctgagcgacg
     1261 cgatgacgcc cgaacagcag gagcggttca ttccggcgtt cgtcaaagat ccggaggcga
     1321 cgttcgccat cgggttcacg gagacaggcg ccggatcgga ccaccagggt tattacgact
     1381 cgcccgatat cgcattccac accaccgcgg tgcgcgacgg cgattcgtac gtgatcaacg
     1441 gcgccaagcg ttatgtctcg aacggctgca tggcgaagct ctatttcgtc gtcgcgcgca
     1501 ccgacacgaa gaaaagcctg cgcgagggcg gcagcgtctt catcgtgccg tccgacacgc
     1561 cgggctttcg cgccggctcc tttcacgaga agagcagcca gcggctcgcg accaatggca
     1621 cgttccactt cgagaactgc cgcgtgccgg cgagccatct tctgggcgga gaagggctca
     1681 tgtcgcggtt gcgcgccgat tacatgtggg gcagcaaggc ggaagcggcg gcgaccgcgc
     1741 tcggtgtcgg gcgcgcggcc tacgagtacg cgctcgcgta tgcgaaagag cgcgtgcagg
     1801 gcggcaagcc tatcgtcgaa ctcgcgcccg tggcgatgat gctcgcgcag atggcgatga
     1861 agatcgatgc ggcgcgcacc cagatctggc gcgcggcgtg gcttgccgag cgcaggcagc
     1921 ctgaagcccg cacgctcggc ctgctcgcca aggtgaacgc gtccgaaacc gcgttcgaag
     1981 cgtgcaagct ggcgtgcgaa att
//
LOCUS       DASURG010000598         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_1122980, whole genome shotgun sequence.
ACCESSION   DASURG010000598 DASURG010000000
VERSION     DASURG010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122980"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="VFY80_10820"
     CDS             complement(<1..1928)
                     /locus_tag="VFY80_10820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX6008448.1"
                     /translation="MTNPSLTALNAEQRAAAVFGASGAKGWTAGPLLIIAGAGTGKTN
                     TLAHRVAHLVLHDVAPERILLLTFSRRAAEEMIRRVRRIVAATATQSAVDGSRVVARL
                     AWAGTFHAIANRLLRQYAVPLALQPGFSVMDRGDSADMMDVARQELGFAVKDKRFPRK
                     DTCLAIYSHCINTRAPLERVLATAFPWCASWHDELKALFRRYAATKLEQQALDYDDLL
                     LYWHALMEVEVLAREVSARYDHVLVDEYQDTNMLQAEILYALKPCGEGLCVVGDDAQS
                     IYSFRAASVENILDFPVRFAPPACVVTLEQNYRSTQPILDAANALIAEADRQYRKRLR
                     AQASSGARPRHVTVVDDQAQAGYVVARILEAREQGVLLRRQAVLFRSAHHSDVLELEL
                     VRREIPYVKYGGLKFLEAAHVKDLLAVLRWADNPRNRMAGFRVMKLVPGVGPGYAERA
                     YVAFEAAAHRWSALGSYAVPTGARAEWTALAELLEAIAAGAWEGQVGRVRHWYEPQLA
                     RLYDAAEARAGDLEQLERIATQFPTRERFLTELALDPPQATGDWSGPPLLDEDYLVLS
                     TVHSAKGQEWDRVYVLNVTDGAFPSEFATGRAEMVEEERRLLYVAMTRARHELDLIAP
                     LKFYLTQQPRMADGHVYGV"
BASE COUNT          367 a          690 c          658 g          341 t
ORIGIN      
        1 acgccgtaga catgcccgtc cgccatgcgc ggctgttgcg tgaggtagaa cttgagcggc
       61 gcaatgaggt caagctcgtg ccgcgcgcgc gtcatcgcca cgtacagcag ccggcgctct
      121 tcctccacca tctcggcgcg gccggtcgca aactcagagg gaaatgcgcc atccgtcacg
      181 ttcagcacat acacccggtc ccactcctga cctttcgccg aatgcaccgt ggacagcacg
      241 agatagtctt catcgagaag cggcgggccg ctccaatccc cggttgcctg cggcggatcg
      301 agcgcaagct cggtgaggaa gcgttcgcgc gtcggaaatt gcgtcgcgat gcgctcgagc
      361 tgctcgaggt cgcccgcccg cgcttctgcg gcatcgtaaa ggcgcgcgag ctgaggctcg
      421 taccaatgcc gtacgcgccc aacctgtcct tcccaggcgc cggcagcgat ggcctcgagc
      481 aattcggcga gcgcggtcca ttccgctcgg gcgcccgtcg gtaccgcata ggatccgagc
      541 gcgctccatc gatgggcggc ggcctcgaac gcgacgtacg cccgctccgc atacccggga
      601 ccgacgccgg gcacgagctt cattacgcga aagcccgcca tgcgatttct cggattgtcg
      661 gcccagcgca gcaccgcgag gagatccttg acatgcgcgg cttccagaaa cttgagcccg
      721 ccatatttca catacgggat ctcgcggcgc acgagttcga gctcgagtac atcgctgtga
      781 tgcgcactgc ggaagagcac cgcctgccgg cgcagcagca cgccctgctc gcgtgcttcc
      841 aggatgcggg caaccacgta cccggcctgc gcctgatcgt ccacgaccgt gacgtggcgc
      901 gggcgcgcac ccgaagacgc ctgcgcacgc aagcgcttgc gatattgacg gtccgcctcg
      961 gcgatgagcg cgttcgccgc atcgagaatc ggctgcgtcg aacggtagtt ctgctccagc
     1021 gtgacgacgc acgcaggcgg cgcgaatcgc accgggaaat cgaggatgtt ctcgacactc
     1081 gccgcgcgaa aagagtagat tgactgcgca tcgtcaccca cgacgcacaa cccttcacca
     1141 cacggcttga gcgcgtagag aatttccgct tgcagcatat tggtgtcctg atactcatcg
     1201 accagcacgt ggtcgtagcg agcggagact tcgcgagcca gcacctccac ttccatcagc
     1261 gcatgccagt agagcagaag gtcgtcgtag tcgagcgcct gctgctcgag cttcgtcgcg
     1321 gcgtagcggc ggaagagcgc cttcagctca tcgtgccatg acgcgcacca cgggaaggct
     1381 gtcgcgagta cgcgctcgag cggcgcgcgc gtattgatgc agtgcgagta gatggcgagg
     1441 caggtatcct tgcgcgggaa acgcttgtcc tttacggcaa accccagctc ctggcgtgcc
     1501 acgtccatca tgtcggccga gtcgccccgg tccatcacgc tgaatcccgg ctgcagcgcg
     1561 agcggcacgg cgtactgtcg cagaaggcgg ttcgcgatgg cgtgaaaggt gcccgcccag
     1621 gcgaggcgcg ccaccacccg cgagccgtcc acggcgcttt gcgtggcggt ggccgcaacg
     1681 atgcggcgca cgcggcgaat catctcctcg gccgcacgcc gcgaaaacgt gagcagcaga
     1741 atgcgctcgg gcgccacgtc atgaagcacg agatgtgcca cacggtgcgc gagcgtgttg
     1801 gtcttgccgg tccccgcccc ggcgatgatg aggagcgggc cggccgtcca gcccttcgcg
     1861 ccggacgcac cgaaaacggc cgccgcacgc tgttccgcgt tgagcgctgt gagcgatggg
     1921 ttcgtcatgc cgccgattat ggcgcagacc tgtatggaca tccagtatcc cggcggggga
     1981 cgttacggaa ctgcatgcgg catcgatgat cgtaccgtct ttactgctcg aactggaggc
     2041 atcatgaggg cgctct
//
LOCUS       DASURG010000599         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_510191, whole genome shotgun sequence.
ACCESSION   DASURG010000599 DASURG010000000
VERSION     DASURG010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510191"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFY80_10825"
     CDS             <1..917
                     /locus_tag="VFY80_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6008449.1"
                     /translation="RHPLMEDPVFRYFFGDPLGSQTQKSTNLGSGVIVSERGFILTNS
                     HVVEAAEEIEVMLADNRRARARVVGSEPETDIAVLKVDLAGLPAITFGHPEEARVGDV
                     VLAIGNPYGFGSTVTLGIVSALGRSNLPINVPFQNYIQTDAAVNPGNSGGALVDISGS
                     LIGINSSIYSSTGSSVGIGFAIPASVAKDVLEQIVASGDVTRGWIGVGAQDMTSEIAR
                     SFDLPDAKGALISEVVNGSPADKAGIKPGDILVAVNGKPVGGSNAMLELIAALKPGQD
                     ARLRVVRERGEKELIATVGRRPRPAQQK"
     gene            complement(928..1734)
                     /gene="tatC"
                     /locus_tag="VFY80_10830"
     CDS             complement(928..1734)
                     /gene="tatC"
                     /locus_tag="VFY80_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6008450.1"
                     /translation="MSEDTFVSHLVELRDRLIRALFAVGLVTAILSIWPGIGGVYDLL
                     ARPMTEALPEGTRMIATGVVSPVFVPLKVTVLAAFLIALPYVLYQAWAFVAPGLYAHE
                     KRVALPLVVSSTALFFLGIAYCYFIVFHLIFHAIQSFAPAAITPAPDIEAYLSFVITM
                     FLAFGITFEIPLLQLVLVRTGVVTVEKLKEIRGYVIVGAFVIAAVVTPPDVLSQLLLA
                     IPMCVLYQVGIYAAGFVRRREEDDPDPYRPLSDAEMESELDKIEADEKSR"
     gene            complement(1926..2153)
                     /gene="tatB"
                     /locus_tag="VFY80_10835"
                     /pseudo
     CDS             complement(1926..2153)
                     /gene="tatB"
                     /locus_tag="VFY80_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
BASE COUNT          415 a          696 c          703 g          371 t
ORIGIN      
        1 cgcggcatcc gttgatggag gacccggtgt tccgctactt cttcggcgat ccgctcggga
       61 gccagacgca gaagagcacc aatctaggct ccggcgtcat cgtgagcgaa agaggcttca
      121 ttctcaccaa cagccatgtc gtggaggcgg cggaggaaat cgaagtgatg ctcgcggaca
      181 accgccgcgc tcgcgcacgc gtcgtcggtt ccgagcccga aaccgacata gcggtgctca
      241 aggtcgatct cgccgggctt cccgcaatca cgtttggaca tccggaagag gcacgcgtgg
      301 gagacgtcgt gctcgccatt ggcaatccct acggcttcgg ctccaccgtc acgctcggca
      361 tcgtgagcgc actcgggcgc tcgaatctgc cgatcaatgt cccgttccag aattacatac
      421 agaccgacgc cgcggtcaat ccggggaatt cgggcggcgc cctcgtcgat atcagcggca
      481 gcctgatcgg catcaactcc tcgatctatt ccagcaccgg gagcagcgtc ggcatcgggt
      541 ttgcgattcc ggcgagcgtt gcgaaggacg tgctcgagca gatcgtcgca agcggcgacg
      601 ttacgcgcgg ctggattgga gtcggcgcgc aggacatgac gagcgagatc gcccggtcct
      661 tcgatcttcc cgacgccaaa ggtgcgctta tttccgaagt ggtgaacggc agccctgcgg
      721 acaaggccgg catcaaaccg ggggacattc tggtcgccgt gaatggcaag ccggtcgggg
      781 gctccaacgc catgctcgag ctcattgccg cgctcaagcc cgggcaggac gcgcgtctca
      841 gggtggtgcg cgaacggggc gaaaaagaac tcatcgcgac cgtgggacgc cggccgcggc
      901 cggcgcagca gaagtagtcc tgcgctctca gcgactcttt tcgtccgctt cgatcttgtc
      961 cagctcgctt tccatttctg cgtccgacag cggtcgatac gggtcggggt cgtcttcttc
     1021 gcgccggcgc acgaagcctg cagcgtagat tccgacctga tagagcacgc acatcggtat
     1081 cgcaagcagc agctgcgaca gcacgtcggg cggcgtcacg acagcggcaa tcacgaaagc
     1141 gccgacgatc acgtaacccc ggatctcctt cagcttctcc acagtcacga cgccggtgcg
     1201 caccagcacg agttgcagca acggtatctc gaacgtgatg ccgaaggcga ggaacatcgt
     1261 gatgacgaag ctcaaatagg cttcgatatc cggcgccggc gtgatcgccg cgggcgcgaa
     1321 cgactgtatc gcatgaaaga tgaggtggaa tacgatgaag tagcagtagg cgatgccgag
     1381 aaaaaacagc gccgtgctgc tcacgacgag aggcagcgcc acgcgctttt cgtgcgcgta
     1441 gaggcccggt gccacgaacg cccacgcctg gtagagcaca tagggaagcg cgatcagaaa
     1501 cgccgcaaga accgtgacct tgagcgggac gaagaccggc gacaccacgc cggttgcaat
     1561 catgcgcgtg ccttcgggca gcgcctcggt catgggccgg gcgaggagat cgtacacacc
     1621 tcctatcccc ggccagatgg agaggatcgc ggtcaccagc cccaccgcga acagcgcgcg
     1681 gatgaggcgg tcacgcaact ccaccaggtg cgaaacgaag gtgtcttcgc tcatgcggcc
     1741 gccgcggccg gcgatcagcc cggcctgcgt tccggcgcct gcgccgggac gtctgtcgcg
     1801 cgcacaggtt cctgcggtgc gggcgactgc gcggtttccg ccggctgcga tgcgcgtgcg
     1861 ctggcggcct cgtcctcagt gattttcctc aattcggttt ccgtttcgtt gacttcttgg
     1921 gataccgagc gctccagcga gcgcgccgcg tcctgcatcg acgcctggag tttcctcagc
     1981 tcgtcgagct ccatctcccg gctgatatcc gccttgacgt cgttgacgta gcgctgcatg
     2041 cgcccgaaga ggtggcccaa ggtgcgcgcc gctttaggaa gccgttccgg gccgatgacc
     2101 agcaacgcca ccacggcgat gacgaggagc tcggagaaac cgatatcgaa catgccagtg
     2161 cgccgggcgc gagggtctgg cgcta
//
LOCUS       DASURG010000600         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_510298, whole genome shotgun sequence.
ACCESSION   DASURG010000600 DASURG010000000
VERSION     DASURG010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510298"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFY80_10840"
     CDS             <1..522
                     /locus_tag="VFY80_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6008451.1"
                     /translation="ALAYVDTHERLYFYAAAATAPAAFLFDVLDGRIARWRQQHSPLG
                     RELDSLADVVSFGVAPATLGFAAGLRGGWDWVVLIYFVCCGVSRLARFNVTAEQFAAE
                     SGKVAYFEGTPIPTSVVLTGVLAFAAAQDRLGESLYWGIWHVGPWVLHPLALLFALSG
                     TLMISKTLRIPKF"
     gene            complement(553..1623)
                     /locus_tag="VFY80_10845"
     CDS             complement(553..1623)
                     /locus_tag="VFY80_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6008452.1"
                     /translation="MPTDRKYVKDADIIFTGCSVTQAAGEPATGGRRRQVYVGGKRVK
                     TIDVHCHCVIPETLAMMGKTPSANEMRGPGITEVGMRRIREMDEQGIDVEAISINPFW
                     YTATRDVAEKLIKTQNDKLTELCAAHPDRFVGFASVALQFPDLAIQQLEYAIKKLGMR
                     GVAVGASVAGEEFADQKFHPFWAKCEELGILIFIHPQSTPDLAKRFKGNGWLANTIGN
                     PLDTTIALSHLIFEGTLDKFPGLKICSAHGGGFLPSYAPRSDNALRVAPDMDTGVKLK
                     KKPTEYLRTMYYDTLVFTSEALRHLAAEVGVSQLVIGTDHPIPWQDKSVDHILNTPGF
                     TDEERRAMLGETAAKLLGIPLA"
     gene            1772..4021
                     /locus_tag="VFY80_10850"
     CDS             1772..4021
                     /locus_tag="VFY80_10850"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6008453.1"
                     /translation="MRLITGAGTYAADWNAAGQLYAYFVRSDRAHAHIVRIDDARART
                     APGVKGVFTGADAVAAGYVRAPHSLNFPGRNGMQARAPDRPALAHGQVRYVGEAVALV
                     VADSPAQAQDAAELIEVEYRDLPCVVDAEEALGPKALQIHDDVPGNLVFEFETGKAQD
                     VEAAFARAVHITRLKVAVSRVAPNPLEPRACLVSYDPATANYSFRTCMQGTTTLRKQL
                     STWTRVPEAHLKFEARDVGGGFGQRTLAYPEYCALMIAAKACGRPVKWISTRMEGFLA
                     DSHGRSNVIAGALALDGDGKFLAMRLDWINDKGAYLSPGAIGHIRNTTTCMTGVYRIP
                     ALYGTYRVALTNCTPIAAYRGAGRPDIAYVVERLVNQAAAELNLDAAELRRRNFIPPD
                     AFPYRTPTGSVYEIADFSGLLGKALELADWRGFPARRARSAATGKLRGIGISTVIENT
                     GAGNAPKDEIEIRVDAKGAITVYTVSKAQGHSHETTLAMIVADALGVPSNRVRIVQCE
                     YDTPLEGNHTGGSRTTVGAGSVCFLAAQKLIEQGKALAALELKLEPSQVAYAHGQFSS
                     SESGRALGFGDLAKEKTLSVRAEGKFGSTFPNGCHIAEVEIDPETGATTIVSYCAVDD
                     CGKVIHHGVVEGQLHGGVAQGVGQIFGEQIAYQSETGQPLAASFMDYAMPRAGWLPAI
                     RGAEHPTPSKVSPLGVKGMGESGCTASLPVLANAVMDALRQRGIEHLDMPFTPSKVWH
                     ALHAAKKRP"
BASE COUNT          700 a         1341 c         1323 g          714 t
ORIGIN      
        1 gcgctggcgt acgtcgatac ccacgagcgc ctctatttct acgccgccgc tgccacggcg
       61 cccgcggcct tcctcttcga tgtcctcgac ggccgcatcg cgcgctggcg ccagcaacac
      121 tcgccgctcg gccgcgagct cgattcgctc gccgatgtcg tttcgtttgg cgtcgcgccc
      181 gcgacgctcg gcttcgcggc gggattgcgc ggcggatggg actgggtcgt gctgatctac
      241 ttcgtctgct gcggtgtgag ccggcttgcg cgattcaacg tgacggcgga gcagttcgcc
      301 gccgaaagcg gcaaagtggc ctacttcgag ggcacgccga tcccgaccag cgtcgtgttg
      361 acgggcgtgc tcgccttcgc cgccgcgcag gaccgtctcg gcgagagcct ctactggggt
      421 atctggcacg tggggccgtg ggtgctgcac ccgctcgcgc tgctcttcgc cctctccggc
      481 acgctgatga tcagcaagac gctgcgcatt cccaagttct gacgccggca cagcatccca
      541 gctgtagacc catcacgcga gcggaatgcc cagcagcttg gccgcggttt cgcccagcat
      601 ggcccggcgc tcctcgtcgg taaagccggg cgtgttgagg atgtgatcca ccgacttgtc
      661 ctgccagggt atcggatgat cggtgccgat gacgagctgg ctcacgccga cctcagcggc
      721 gagatgccgc agcgcctccg aagtgaatac cagcgtgtcg tagtacatcg tgcgcagata
      781 ctcggtgggc tttttcttga gcttgacacc ggtatccatg tcgggcgcga cccgcagcgc
      841 gttgtccgag cgcggcgcgt acgaaggcag gaaaccaccg ccgtgcgcag agcagatctt
      901 cagccccggg aatttatcca gggtgccttc gaagatgaga tgcgagagcg cgatagtcgt
      961 gtcgagcggg ttaccaatgg tgttcgcgag ccagccgttg cccttgaagc gcttcgcgag
     1021 atccggcgtg ctctggggat ggatgaagat gaggatgccc agctcctcgc acttcgccca
     1081 gaacggatga aatttctgat cggcaaattc ctcgccggcg acgctggcgc ctaccgcgac
     1141 accgcgcatg ccgagcttct tgatggcgta ttcgagctgc tggatcgcga ggtcgggaaa
     1201 ctgcagcgcc accgaggcaa agccgacgaa gcggtccgga tgcgcggcgc agagctccgt
     1261 cagcttgtcg ttctgggtct tgatcagctt ctccgccaca tcgcgcgtcg cggtgtacca
     1321 gaacgggttg atgctgatcg cctcgacgtc gatcccctgc tcgtccatct cgcggatgcg
     1381 gcgcataccc acttccgtga tccccggccc gcgcatctcg ttcgcgctcg gcgtcttgcc
     1441 catcatggcg agcgtctccg gtatgacgca atggcagtgc acgtcgatcg tcttgacacg
     1501 cttgccgccg acgtacacct gacgccgccg gccgccggtc gcgggctcac ccgcggcttg
     1561 cgtcacgctg caaccggtga agatgatatc ggcatccttg acgtacttac ggtcagtggg
     1621 catcttgctc tcctgattgg aatccggatt cggggacagg ccccgggcgg ggtctctcac
     1681 cggatgaacg atcgcggcag attatcgcat agggctagac atcatggaca cgcatgcttc
     1741 gcatcacgga catggagaac gcgtagagga tgtgcgtctc atcaccggcg ccggcacata
     1801 cgcagcggac tggaacgcgg cagggcagct ctacgcctat ttcgtccgct ccgaccgggc
     1861 acatgcgcat atcgtgcgca tcgatgacgc acgcgcgcgc accgcgcccg gcgtgaaagg
     1921 tgtcttcacc ggcgcggatg cggtcgcggc ggggtacgtg cgcgcgcccc actcgctcaa
     1981 ttttcccggc cgcaatggca tgcaggcccg cgcaccggac cggcccgcac ttgcgcacgg
     2041 ccaggtacgc tacgtgggtg aagcggtcgc gctcgtcgtt gcggattcgc cggcacaggc
     2101 acaggacgcg gccgagctga tcgaggtgga ataccgggac ctaccgtgcg tcgtggacgc
     2161 ggaagaggcg ctcggtccga aggcactgca gatccacgac gacgtgccgg gcaatctcgt
     2221 cttcgagttc gagaccggga aggcgcagga cgtggaagcg gcatttgccc gtgccgtgca
     2281 cattacgcgg ctcaaggtcg cggtgagccg ggtggcaccg aaccccctgg agccgcgcgc
     2341 ctgcctcgtc agctacgatc ccgcaacggc gaactattcc ttcaggacgt gcatgcaggg
     2401 caccactacg ctgcgcaaac agctctcgac gtggacccgc gtgcccgaag cgcatctcaa
     2461 gttcgaagcg cgcgacgtcg gcggcggctt cggccagcgc acgcttgctt atcccgagta
     2521 ctgcgcgctc atgatcgcgg cgaaggcatg cgggcgaccg gtgaaatgga tttccacgcg
     2581 gatggagggc tttctcgccg acagccacgg ccgctccaac gtcattgccg gcgcgctcgc
     2641 gcttgatggg gacgggaaat tcttggcgat gcggctcgac tggatcaacg acaaaggcgc
     2701 ctacctttca cccggcgcga tcggccacat tcgcaatacg acgacgtgca tgacgggggt
     2761 ctaccgcatc cccgcgctct acggcacgta tcgtgtcgcg ctgacgaatt gcacgccgat
     2821 cgcggcgtat cgcggcgcgg gacggcccga tatcgcttac gtcgtcgaaa ggctcgtcaa
     2881 tcaggcggcg gcggagctca acctggatgc cgctgagttg cggcggcgca atttcattcc
     2941 accggatgcg tttccctatc gcaccccgac cggctcggtc tacgagatcg ccgacttctc
     3001 cggcctgctc gggaaggcgc tcgagctcgc ggactggcgg ggctttccag cgcgtcgcgc
     3061 gcggtcggcg gcgaccggga aactgcgcgg catcggcatc tctaccgtga tcgagaatac
     3121 cggcgccggc aacgcgccca aggacgagat cgagatccgc gtcgatgcga agggcgcgat
     3181 cacggtctac accgtgtcca aggcgcaggg gcacagccac gagacgacgc ttgccatgat
     3241 cgttgccgac gcgctcggcg tgccgagcaa tcgagtgcgc atcgtgcaat gcgagtacga
     3301 cacgcccctc gaaggcaacc acaccggcgg ctcgcgcacg acggtcggtg ccggcagtgt
     3361 gtgttttctt gcggcgcaga agctcatcga acagggcaag gcgctcgccg cgctcgaact
     3421 caagctcgag ccctcacagg tggcgtacgc gcacggccag ttctcgtcgt cggaatcggg
     3481 acgggcgctc ggtttcgggg acctcgcaaa agaaaaaacg ctcagcgtca gggcagaagg
     3541 caagttcggc tcgacgtttc cgaacggctg ccacatcgct gaagtcgaaa tcgatccgga
     3601 aaccggcgca acgaccatcg tttcctattg cgccgtcgac gactgcggca aggtcatcca
     3661 ccacggcgtg gtggagggcc agctccacgg cggcgtcgcg caaggcgtgg gacagatctt
     3721 cggcgagcag atcgcctatc aatcggaaac cgggcagccg ctcgccgcga gcttcatgga
     3781 ttacgcgatg ccccgcgcgg gatggctgcc cgccattcgc ggggcggaac accctacgcc
     3841 gagcaaggtg agtccgctcg gcgtcaaagg catgggcgaa tccggctgca cggcttcact
     3901 tccggtgctc gccaatgcgg tgatggatgc gctgcgccag cgcggcatcg agcacctcga
     3961 catgccgttc acgccgtcca aggtctggca cgcacttcac gccgccaaga aacgtccctg
     4021 attatcgtca ttcgggtgca cgccgcctga cctgtcactg cgcgcagtcg agaaaacc
//
LOCUS       DASURG010000601         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_102012, whole genome shotgun sequence.
ACCESSION   DASURG010000601 DASURG010000000
VERSION     DASURG010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102012"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFY80_10855"
     CDS             <1..952
                     /locus_tag="VFY80_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6008454.1"
                     /translation="FLCAFFVFAAPAMALAAASHYPARPVRIVVPVSAGGGVDSLARL
                     IAQHYNAVWGQPFIVDNRTGAGGATGVEIAAKAAPDGHTLLVSSSSLVTNAAVREVRY
                     DPVHDFQPVTKLTANPYILLVTPALAVSSVKQLIALAQAHPGKLNYASSGTGGVLHLG
                     AELLCALSGVRMTHVPYKGVADGYPAVISGEVSWMLGSPISALPLMKAGRLRGIAVTS
                     AARSKALPDLPTIAESGAPGYDVTAWFALFAPARIPPDIVSKLHAEARKALLEPNVLR
                     RMASEATDVVVNAPAEFAPEVRKEYDTWRRLVQQAGLKAQ"
     gene            1011..1292
                     /locus_tag="VFY80_10860"
     CDS             1011..1292
                     /locus_tag="VFY80_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008455.1"
                     /translation="MVLLYDPTAPRAAASSTASRTLDTLHGKVIGFIDNAKPNFDQFV
                     DALAELLTARYGVARVVRHRKRSASIPAEAEALDQLAAECDLVVTGSGD"
     gene            1552..2607
                     /locus_tag="VFY80_10865"
     CDS             1552..2607
                     /locus_tag="VFY80_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6008456.1"
                     /translation="MTSAQAPDRFEAPDDLDAINALYRERRWSDGLPVVPPTVERVER
                     TLEHAGRSRAEIIARVAPAYGAATVEHVAVNAVLAGCAPEFLPVVIAALEAAADPAFN
                     LQGIQATTNPVAVWIIVNGPAAARLGINSTYNCLGHGAWANATIGRALRLCLQNIGGA
                     LPGEMDRATHGQPGKYTFCCAENETRNPWTPLHVERGYARGSSTVTVVGAEGTMNMNT
                     HCKDAADLLRVVAETMVHPASNEYCHGGEPWIVLAPEHADILHQAGYTKSQVKQQLWD
                     QSRMPAGRMSAKDLLRVQASRTDELGEIRPDTLVPIAPRAEDIGLVVAGGPGTHSVYV
                     PSFGNTRAVTREIRFAR"
     gene            complement(2659..>3288)
                     /locus_tag="VFY80_10870"
     CDS             complement(2659..>3288)
                     /locus_tag="VFY80_10870"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6008457.1"
                     /translation="DFTAETTQGTIRFHEWIGEGWAILFSHPKDFTPVCTTELGYMAK
                     LKPEFEKRNCKIIGLSIDPVSDHKRWSDDIRDATGHSPNYPIIGDADLKVAQLYDMIH
                     PNAAAKKEGRTAADNQTVRSVFVIGPDKKVKLILTYPMSTGRNFDEVLRVLDSIQLTA
                     KHKVATPVNWKHGEDVIIVPAVSDDEARQRFPGGWKAPKPYLRIVPQPK"
BASE COUNT          566 a         1078 c         1065 g          579 t
ORIGIN      
        1 ttttctgtgt gcttttttcg tgttcgctgc gcctgccatg gcgcttgccg ccgcgtcgca
       61 ctatccggcc cgtcccgtgc gcatcgtggt accggtgagc gcgggcggcg gtgtggacag
      121 cctcgcacga ctgatcgcgc agcactacaa cgccgtatgg ggccagccct tcatcgtgga
      181 caaccgcacc ggcgcgggcg gggcgaccgg tgtcgagatt gcggcgaagg cggcgccgga
      241 cggccatacg ctgctcgtga gcagcagcag tctcgtcacc aatgccgccg tccgggaggt
      301 gcgctacgac ccggtgcacg attttcagcc ggtcacgaaa ctcactgcca atccgtacat
      361 cctgctcgtc acccctgcac tcgccgtctc ttcggtgaag cagttgatcg cgctcgcgca
      421 agcgcacccg ggcaagctca attacgcgtc ttccggaacc ggcggcgtgc tgcatctcgg
      481 cgcggagtta ctgtgcgcgc tgagcggcgt ccgcatgacg cacgtgccgt acaaaggcgt
      541 cgccgacggc taccccgcgg tcatttcagg agaagtgagc tggatgctcg gaagcccgat
      601 ctccgcgctg ccgctcatga aggcgggacg cctgcgcgga atcgccgtta cttcagccgc
      661 acgcagcaag gcgctgcccg atctgccgac catcgccgag agtggcgctc cgggttacga
      721 tgtgaccgca tggttcgcat tgttcgcgcc ggcgcgcatc ccgcccgaca tcgtctcgaa
      781 gctccacgcg gaggcgcgca aggcgttgct cgagccgaac gtcttgcggc gcatggcgag
      841 cgaagcaacg gacgtcgtgg tcaatgcgcc cgccgaattt gcaccggaag tgcgaaaaga
      901 atacgacacg tggcgccgac tcgtgcagca ggcagggctg aaagcccagt gacgaatcgc
      961 ttgaagcgcg gagcggttct gcggatactc tttttttcta gaggcaggac atggtactcc
     1021 tttacgatcc caccgcgccg cgagcggcgg ctagcagcac cgcatcccgg accctcgaca
     1081 cgctgcatgg caaagtcatc ggtttcatcg acaacgcaaa gcccaacttc gatcagttcg
     1141 tggatgcgct cgcagagctg ctcacggcgc gatatggcgt cgcgcgtgtc gtgcggcatc
     1201 gcaagcgctc ggcttcgatt ccggccgaag cagaagccct cgaccaactt gccgcggagt
     1261 gcgatctcgt tgtcacgggg tctggcgact gaggctcatg cgcgtcgtgg agtgtccacg
     1321 acagtgtcga agcgatcaag cgggggcggg ccgccatcgc agtctgttcg agtgcgttcg
     1381 tcacgcttgg ccgcgcgcag gctgccgcac tcggccaacc ggcgctcccg atcgctgtga
     1441 tcccgcatcc cttcggaaac cgtacgccgg acgaagtgcg ggccatcgcg gcgcagtgcc
     1501 tgcccgacat cgtggcgttc gcatgtcaag ccgtgcccgt cgagtcttag ggtgacctcc
     1561 gctcaggccc cggaccggtt cgaggcgccc gacgatctcg acgccatcaa cgcgctctat
     1621 cgcgagcgcc gctggagcga cggcttgccg gtcgtccccc cgaccgtcga gcgtgtcgag
     1681 cgcacgctag agcatgccgg gcgatcccgc gcagagatca tcgcccgcgt ggcgcccgct
     1741 tacggcgccg caacggtaga gcacgtggcg gtaaacgccg tactcgccgg ttgcgcgccg
     1801 gaatttctgc cggtcgtgat cgcggcgctg gaggctgcgg ccgaccccgc gttcaatctg
     1861 cagggcatac aggctacgac caatcccgtc gcggtgtgga tcatcgtcaa cggtccggcc
     1921 gccgcgcgac tcggcatcaa ctcgacctac aactgcctgg ggcacggcgc gtgggcaaat
     1981 gcgacgatcg ggcgcgcgct gcggctctgc ctgcagaaca tcggtggcgc gctgccggga
     2041 gaaatggatc gcgcaacgca cggacagcct ggaaagtaca cgttctgctg cgcggaaaac
     2101 gaaacgcgga acccgtggac gccgctgcac gtggaacgcg gttatgcacg cggatcgagc
     2161 accgtcaccg tcgtcggcgc ggagggcacg atgaatatga acacccactg caaggacgcg
     2221 gccgatctgc tgcgtgtcgt cgcggagacc atggtgcatc ccgcgagcaa cgaatactgt
     2281 cacggcggtg aaccgtggat cgttctcgcg cccgagcacg cggacatcct gcaccaggcc
     2341 ggctacacca agtcgcaggt gaagcagcag ttgtgggacc agagccgcat gccggccggg
     2401 cgcatgtcgg caaaggatct cctgcgggtt caggcatcgc gcacggacga actgggcgag
     2461 atccggcccg acacgctcgt gcccatcgca ccgcgagccg aggatatcgg actcgtcgtc
     2521 gccggcggac ccggcacgca ctccgtgtac gtaccctcct tcggcaacac gcgcgcggtc
     2581 acccgcgaga ttcgcttcgc aaggtagccg catcacaaag cccggccgca agaccggact
     2641 cggtacagcg cggccggctt acttcggttg cggcacgatg cgcaagtagg gcttcggcgc
     2701 cttccagccg ccagggaagc gctgccgcgc ctcatcgtcg gagaccgcgg gaacgatgat
     2761 gacgtcttca ccatgcttcc agttcaccgg agtggccact ttgtgcttgg cggtgagctg
     2821 gatcgaatcg agcacgcgca atacctcgtc aaaattgcgg ccggtgctca tggggtaggt
     2881 gaggatcagc ttcaccttct tgtcgggacc gatcacgaag accgagcgca cggtctggtt
     2941 gtcggccgcg gtcctgccct ctttcttggc ggccgcgttg gggtggatca tgtcatagag
     3001 ctgcgccacc ttgagatccg catcgccgat gatcggataa ttgggtgaat gtcctgtggc
     3061 gtcccgtata tcgtcggacc agcgcttgtg gtcgctgacc gggtcgatgc tgaggccgat
     3121 gatcttgcaa ttgcgcttct cgaactccgg cttgagcttg gccatgtagc cgagctcggt
     3181 cgtgcagacc ggcgtgaaat ccttggggtg ggaaaacaat atcgcccagc cctctccgat
     3241 ccactcgtga aaacgtatcg tgccctgcgt cgtctccgcc gtgaaatc
//
LOCUS       DASURG010000602         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1701
            k141_816682, whole genome shotgun sequence.
ACCESSION   DASURG010000602 DASURG010000000
VERSION     DASURG010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961837
            Sequence Read Archive: SRR25158508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:38:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,162
            Genes (coding)                    :: 2,151
            CDSs (with protein)               :: 2,151
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816682"
                     /isolate="SMAG_U1701"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.83166667 N 104.6163889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFY80_10875"
     CDS             complement(<1..921)
                     /locus_tag="VFY80_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6008458.1"
                     /translation="MRLRSLLPPDTRPDAAYILWSRALRAFGDGYMSLLLPFYLPLLG
                     YSALEVGVLVTATLLGSGAMTLAVGFIAHRFPGRTLLQAASLLMAVTGAAFVLATEFW
                     PLLLIAFVGTLNPSSGDVSVFLPLEQALLAHTVTAKSRTALFARYSLIGALVGAIGAQ
                     AAGIPALLEASFQVELKLALQAMFLLYGALGVVSLLLYRSIAPTFEADQQARPSPLGK
                     SRPTVLKLAALFSLDAFAGGFAVQSLLALWLFERFELSVATAGTVFFWVGILSALSQL
                     ASARIAAQFGLVNTMVFTHLPSSIFLIVVPF"
     gene            1045..1740
                     /locus_tag="VFY80_10880"
     CDS             1045..1740
                     /locus_tag="VFY80_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX6008459.1"
                     /translation="MLEVYSWPTPNGHKVHIMLEETGLPYRVHGINIRQGDQFKPEFL
                     KISPNNRIPAIVDPDGPNGEPLSLFESGAILLYLASKTGRLLPIDMHARWTCVQWLMW
                     QMGGIGPMMGQANHFRRYAKEKIQYAIDRYTNEANRLTHVLDRRLKEAQYLAGDEYSI
                     ADIAVFPWMRGAENRGVDIAEYPNVERWFNAINARPAVQRGVQVLATGHETQPHDDKS
                     WEIMFGKVQFQRR"
     gene            1919..>3345
                     /locus_tag="VFY80_10885"
     CDS             1919..>3345
                     /locus_tag="VFY80_10885"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX6008460.1"
                     /translation="MNQPAVAAHVAHSADIPLYVHHARLAQWVRDIAQLTKPDRVYWC
                     DGSQEEYDRLCTEMVASGMLIKLNQEKRPGCFLARSDPDDVARMEERTFVCSRNREDA
                     GPNNNWVAPAEMRATLGELFDGCMRGRTMYVIPYSMGPLGSHIAHIGVELTDSPYVVA
                     SMRIMTRIGRQVLDVLGRDGYFVPCVHSVGMPLAAEQKDVPWPSNKEHKYIVHYPETK
                     EIWSFGSGYGGNALLGKKCFALRIASIMAREQGWLAEHMLILGIQPPKGPKHYIAAAF
                     PSACGKTNLAMLIPPKALKGWKVTTVGEDIAWIKPGKDGRLYAINPEAGAFGVAPGTS
                     DKTNRNCMAAVTHNTIFTNVAMTPDGDVWWEGMTKKPPRKLIDWQGREWTPKSGTKAA
                     HPNARFTVHSSQIPSLDPEWENPDGVPISAFLFGGRRSTTVPLITQAFNWVHGVYLAA
                     TMASETTAAIVGKVGVVRRDPFAMLP"
BASE COUNT          709 a          991 c         1098 g          547 t
ORIGIN      
        1 gaacggtacg acgatcaaaa aaatgctgga gggcagatgc gtgaaaacca tggtattgac
       61 gagcccgaac tgggcggcga tccgtgctga ggcaagttgc gagagcgcgc tcaggatgcc
      121 gacccagaaa aatacggtgc ccgccgttgc gacggaaagc tcgaatcgtt cgaaaagcca
      181 cagggcgagc agcgactgca cggcgaagcc gcccgcgaag gcatcgaggc tgaagagcgc
      241 ggcgagcttg agcaccgtgg ggcgcgattt gccgagaggc gaagggcgcg cctgctgatc
      301 ggcctcgaac gtcggcgcaa tgctgcggta cagaaggagg ctcacgaccc cgagcgcacc
      361 gtagagcagg aacattgcct gcagtgcgag cttgagctcg acttgaaacg acgcctcgag
      421 cagcgccggg atgccggctg cctgcgcgcc gattgccccg accagcgcgc cgatcaggct
      481 gtaacgcgcg aaaagcgctg tgcgcgattt ggccgtgacg gtgtgcgcaa gcagcgcctg
      541 ctccaaaggc agaaaaacgc tcacgtcccc gctggaggga ttgagtgtgc cgacgaaggc
      601 gatgagaagg agcggccaga attcggtcgc cagcacgaat gctgcgccgg ttaccgccat
      661 gagaagcgag gccgcctgca gcagcgtgcg cccggggaag cgatgcgcaa tgaatccgac
      721 cgccagtgtc atcgctcccg agccgagcag ggttgcggta acgagcacgc ccacctcaag
      781 agcgctgtag ccgagcagcg ggagatagaa tggcagcagc aggctcatgt aaccgtcccc
      841 gaaggcgcgc agggcacgcg accaaaggat gtaggccgcg tccggtcggg tgtccggagg
      901 aagcaggctt cgaaggcgca tcggtatcct cgcgcgtggg ttcatgcgag caagccttgg
      961 gcagcacgcg ctcgtgcctg cctcaggaag gcggaaaccg tgctggagta tactgcgcgc
     1021 tttcctctac gccaggagaa aaatatgctt gaggtataca gttggcccac gcccaacggg
     1081 cacaaagtgc acatcatgct ggaggagacc gggcttccgt atcgcgtgca cggcatcaac
     1141 atccgccagg gcgatcagtt caaaccggaa ttcctcaaga tcagcccgaa caaccgcata
     1201 cccgcgatcg tcgatccgga cggtccgaac ggagagccac tctcgctgtt cgagtccggc
     1261 gcgattctgc tctatctcgc atccaagacc ggcaggcttc ttccgataga catgcatgcg
     1321 cgctggacct gcgtgcaatg gctcatgtgg cagatgggcg gcatcgggcc gatgatgggc
     1381 caggcgaatc acttccgccg ctacgcgaag gagaagattc agtacgccat cgatcgctat
     1441 accaatgaag cgaaccggct cacccacgtg ctcgacaggc gtcttaagga agcgcagtac
     1501 ttggccgggg acgagtactc gattgcagac attgcggttt ttccatggat gcgcggggcg
     1561 gagaaccgcg gtgtcgacat cgcggagtat ccgaacgtcg agcgctggtt caacgcgatc
     1621 aacgcgcgtc cggcggtgca gcgcggcgtg caggtgctcg cgacgggaca cgaaacgcaa
     1681 ccgcacgacg acaagtcgtg ggaaatcatg ttcggaaaag ttcagttcca gcgtcgttga
     1741 aactgctcgc ttaatttggg gtccatccag agcagccgtt ttgccgcagt gcagaatggc
     1801 gagtgcaagc ttttgcggta gaatcggacc gtccccggca gagcggctcg cgacgagcgc
     1861 tttgccgttg ttatttaacc aatagatgcg tcaggcatcg ttcgggaagg acgaccaaat
     1921 gaatcaaccc gcagtcgcgg cacacgtcgc gcattccgcg gacattcccc tgtatgtcca
     1981 tcacgccagg ctcgcgcaat gggtgcgcga cattgcgcag ctcaccaagc cggatcgcgt
     2041 gtactggtgc gacgggtcgc aggaggagta cgaccggttg tgcaccgaga tggtggcgtc
     2101 gggcatgctg atcaagctga atcaggaaaa gcggcccggg tgctttctcg cgcgctcgga
     2161 cccggatgac gttgcgcgca tggaggaacg cactttcgtg tgcagccgca acagggaaga
     2221 cgcgggtccg aacaacaact gggttgcacc ggctgaaatg cgtgcgacgc tcggtgagct
     2281 gttcgatggc tgcatgcgcg gccggacgat gtacgtgatt ccctacagca tggggcccct
     2341 cggctcgcac attgcccata taggcgtgga attgaccgat tcgccatacg tcgtggcgag
     2401 catgcgcatc atgacgcgca tcggccgcca ggtgctcgac gtgctggggc gggacggcta
     2461 cttcgtgccg tgcgtgcatt ctgtcggcat gccgctcgca gcggagcaga aggatgtacc
     2521 gtggcccagc aacaaggagc acaagtacat cgtgcattac cccgagacca aggagatctg
     2581 gtcgttcgga agcggctacg gcggcaatgc gctgctcggc aagaagtgct ttgcgctgcg
     2641 catcgcctcc atcatggcgc gcgagcaggg ctggctcgcc gaacacatgc tgattctggg
     2701 catccagccg ccgaaggggc cgaagcatta catcgccgca gcattcccga gcgcgtgcgg
     2761 taagaccaat cttgcgatgc tgattccgcc caaggcgctc aagggctgga aggtgacgac
     2821 ggtcggtgaa gacatcgcct ggatcaagcc aggaaaagac ggacggctct atgcgatcaa
     2881 tccggaagcg ggtgctttcg gcgtcgcgcc cggcacctcg gacaaaacca accgcaactg
     2941 catggcggct gtcacgcaca acaccatttt caccaatgtc gcgatgaccc cggacggcga
     3001 cgtgtggtgg gaagggatga cgaagaagcc gccaaggaaa ctcatcgact ggcaggggcg
     3061 cgagtggacg cccaagagcg gcaccaaggc cgcgcatccc aatgcgcgct ttaccgtcca
     3121 ctcgagccag attccatcgc tcgacccgga gtgggagaac ccggacggcg tgccgatcag
     3181 cgcgtttctg ttcggcggac ggcggagcac cacggtcccg ctcatcacgc aggcgttcaa
     3241 ctgggtgcat ggcgtgtatc tcgccgcgac catggcttcc gagacgaccg ccgccatcgt
     3301 aggcaaggtc ggcgtcgtgc ggcgcgatcc gtttgccatg ctgcc
//
